LOCUS       DASPWO010000001         5576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_424410, whole genome shotgun sequence.
ACCESSION   DASPWO010000001 DASPWO010000000
VERSION     DASPWO010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424410"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VEO00_00005"
     CDS             complement(<1..745)
                     /locus_tag="VEO00_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56417.1"
                     /translation="MKLVANPECRRVHYHAPTTLPDGRLGFLKDCDSFQVNERSAFWL
                     IGSEADGSDAMPLMDGPLGYSLSSFTWNPQITEGLFSIGSGICSGIARMTRGGVEDFP
                     ITVGDGNHRFRVDADIRDPSDSDCWNEGQAGSPIWSPDGSRIAFLASPLAVGVEGFNR
                     AYLPWNLYLMDAEGSQARAALSDISSAGGIAWRPQGDWIALIGDPPGYGDGLWLYSLG
                     QDTLRRVTDDPPIGFAWSSDGTEIVGIHVV"
     gene            complement(1052..2803)
                     /locus_tag="VEO00_00010"
     CDS             complement(1052..2803)
                     /locus_tag="VEO00_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYU56418.1"
                     /translation="MIIPVEERIDTLLTANSIHEGLETAATNVDATAHFYLEDGPVSF
                     TISELLSTCGTGRALLADRGVGKGDRVGILGSNDHHWARWAFSVWSAGAVAVPLPVPI
                     RLRDADAISEQVRSLATASGCKAVAAHPMLAWTIPQELLLPWEMPAIADSAEPASGIT
                     RDDIAVIQLTSGSTAFPKGAVLSHHAVLTGCWGGLTSYEASDDMVVVGWLPFFHDMGL
                     FATLVSPFIAGLNVHLLPPERFARAPGEWFRLASRVGATMTIAPPTAWAAAVRAVDRD
                     PGGVEDLSLFTTAALAAESIEPEVVERVLEVGGRFGLPREAVGGAYGLAETTLGVTVT
                     YPGEGPAFDTIDLEILAERGEAIPPRGGGPEKRLTSCGRTMPGVEMRVVGPDGSPLPE
                     RHAGEIHVQSPTLMEGYMDVPEADQPFTDDGWLRTGDIGYVADERLYVTGREKDLLIV
                     LGRNYVPEDIEWAAARVEGVRAGRVVAFGRPGGGDADVIVAVEPTRQADPEKLPLLVR
                     RMISDSVGIRPREVLVLAPGTIEKTTSGKLRRRALRDSYERGALEPLATGPGGVDQRQ
                     FRQSFRGDRRGRLIAIL"
     gene            complement(2800..3048)
                     /locus_tag="VEO00_00015"
     CDS             complement(2800..3048)
                     /locus_tag="VEO00_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYU56419.1"
                     /translation="MRTRSKIEKFVGRELLEGASVDGDPLAMGLLDSLAVEQLVVFLE
                     KEFNVTFTDDELVEENFESINAVVNLVEAKRRGQTVSR"
     gene            complement(3101..3187)
                     /locus_tag="VEO00_00020"
     tRNA            complement(3101..3187)
                     /locus_tag="VEO00_00020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3151..3153),aa:Leu,seq:cag)
     gene            3312..4040
                     /locus_tag="VEO00_00025"
     CDS             3312..4040
                     /locus_tag="VEO00_00025"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HYU56420.1"
                     /translation="MANLRDFERRLGDLVEGFFGKAFRSGLQPVELGRRILREMEAGR
                     TVGVQNVWVPNHYTFHVAAEDRDHFSQVEQAIGKELEGVVLENARERGWGLLGPPEVV
                     FETDPDLKKGTFHCEARLVQAEDVRTGEHAAYLPAAGAGAPAAAGPAALVLIAGGSES
                     GRFPLDRDVVRIGRMADCDVVLEDSGVSRHHAEVRREDGHFVIADLGSTNGTMVNEAQ
                     VSERELEEGDRITIGRSVLEFRRG"
     gene            4045..4503
                     /locus_tag="VEO00_00030"
     CDS             4045..4503
                     /locus_tag="VEO00_00030"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYU56421.1"
                     /translation="MPPFVLTVLKFAFLALLYYFVYRAIRVAVVAVGERRPGRKKGAP
                     PAPRPKARRGGREPGAVVLLAPDGKKQRSFRLTGTVQIGRAEACQIRLDDTYVSQFHA
                     RLFSRNGAWFVEDLGSTNGTYLNQQKVTAPTPVSLGDRLKVGKAVLELRR"
     gene            4500..>5576
                     /locus_tag="VEO00_00035"
     CDS             4500..>5576
                     /locus_tag="VEO00_00035"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HYU56422.1"
                     /translation="MKLSVGAQSDVGRVRERNEDALLLHDPLYAVADGMGGHRAGEIA
                     ARTAVDTLRGEPLGDGAHALAERVRQANRAVLDTAAGDRALEGMGTTLTAVLLAGDEL
                     RIAHVGDSRAYLLRGDAFTQITEDHTLVQRMVRQGRLTPAEAEVHPQRSILTQALGVD
                     PAIEVDELSVPVLPGDRVLLCTDGLTGMLSQEEIEGTLRETPDPQAAADALVAAAVDA
                     GGIDNVTVLVIDLLDDSGGRPALPPAPEEADEAEEAEEAEPEGETGDEPDDTASVERV
                     ARPARAPARPRRWRRAVVAAAAVALLVTGAVAAKALYVDRQWYVGESNGMVAIFRGLP
                     AEPLGLDLSTLVEETGLPAAEVERL"
BASE COUNT          830 a         1992 c         1963 g          791 t
ORIGIN      
        1 cgaccacgtg tatccccaca atctcggttc cgtcagatga ccaggcgaaa ccgatcggag
       61 gatcatcggt gacgcggcgc aacgtatctt ggccgagcga gtacagccaa agtccgtctc
      121 cgtaccctgg cggatcgccg atgagggcga tccagtctcc ctgaggcctc caggcgatcc
      181 ccccggcact gctgatgtcg ctgagcgccg cgcgcgcctg gctcccttcc gcatccatca
      241 ggtagaggtt ccagggaaga taggctcgat tgaacccttc cacccctacc gcaagtggcg
      301 acgcgaggaa ggcaatccgg ctcccgtccg gagaccagat cggagagcct gcctgcccct
      361 cattccagca gtcactgtcg gatggatcgc gtatgtccgc gtcgactctg aacctatggt
      421 taccgtcgcc taccgtgatg ggaaagtcct cgaccccgcc ccgggtcatc cgggcgatgc
      481 cgctgcagat cccgctgccg atgctgaaca gcccctcggt gatttgtgga ttccaggtga
      541 atgagctgag gctgtacccg agcggtccat ccatcaaggg catagcatcg cttccgtcgg
      601 cttccgaccc gatcagccag aaggcggacc gctcgttgac ctggaagctg tcgcagtcct
      661 tgaggaaccc gaggcggccg tcgggcaggg tagtgggcgc atgatagtgg acccttcggc
      721 actcgggatt ggcgacgagc ttcactgggc cgaattcact cccatccggt cgaagacgcc
      781 acagtcgctc cagtgcggtg atccgctcac ttcccggccg ccagttcacg acgatccatc
      841 cgctcgggag ccatgcaacg gctgcgtagt cggcaggttc aaccgggatg cgacgcgagg
      901 caaccccgtc ggcgctaagc ccggatgggc ccaccgagca cccagctgcc accatgagca
      961 caaccagacc gatggccaac cgagggagcc tcatgtcttg cctagaatcc cgcactccga
     1021 taccaatgat ggtgcagcca ctgagtcccg cctagaggat cgcgatcaag cggccacggc
     1081 gatcgccccg aaacgattgc cggaactgtc tctgatccac tccccccggg ccggtggcga
     1141 ggggttccag cgctccgcgt tcgtacgagt cgcgtaacgc ccggcggcgg agcttgccgc
     1201 tcgtcgtctt ctcgatcgtg ccgggggcga ggaccaggac ctcccggggg cggatgccga
     1261 cggagtcgga gatcatgcgc cgcacgagca gcgggagctt ctccgggtcc gcctggcggg
     1321 tcggctcgac ggccacgatc acgtcggcgt cgccgcctcc cggccgcccg aacgcgacga
     1381 cccggcccgc gcgcacgccc tcgacgcgcg cggccgccca ctcgatgtcc tcgggcacgt
     1441 agttccggcc gagcacgatc agcaggtcct tctcgcggcc ggtgacgtac agccgctcgt
     1501 cggcgacgta gccgatgtcg ccggtccgca gccacccgtc gtcggtgaac ggctggtccg
     1561 cctccgggac gtccatgtag ccctccatca gcgtcgggct ctgcacatgg atctcgccgg
     1621 cgtggcgctc ggggagcggc gagccgtcgg gcccgaccac gcgcatctcg acccccggca
     1681 tcgttcgccc gcacgaggtc agccgcttct ccgggccgcc gccccgaggc gggatcgcct
     1741 cgccgcgctc ggcgaggatc tcgaggtcga tcgtgtcgaa ggccgggccc tcgcccgggt
     1801 aggtcaccgt gacgccgagc gtcgtctcgg ccaggccgta ggcgccaccg accgcttcgc
     1861 gcggcaggcc gaagcgcccg ccgacctcga gcacgcgctc gaccacctcc ggctcgatcg
     1921 actcggccgc cagcgccgcc gtcgtgaaga gggagaggtc ctccaccccg ccgggatcgc
     1981 ggtcgacggc gcggaccgcc gcggcccagg ctgtgggcgg ggcgatggtc atcgtcgccc
     2041 cgacgcggct cgccaggcgg aaccattccc ccggcgcccg cgcgaaacgc tccgggggca
     2101 gcaggtgcac gttcaggccg gcgatgaacg gtgagacgag cgtggcgaac aggcccatgt
     2161 cgtggaagaa cggcagccag ccgacgacga ccatgtcgtc gctcgcctcg tacgacgtca
     2221 ggccacccca gcagcccgtc agcaccgcgt ggtgcgagag cacagcgccc ttcgggaacg
     2281 ccgtgctccc ggaggtcagc tggatcaccg cgatgtcgtc gcgggtgatc cccgacgccg
     2341 gctccgcgct gtcggcgatg gccggcatct cccacggcag cagcagctcc tgggggatcg
     2401 tccaggccag catcgggtgc gccgccaccg ctttgcatcc cgaggccgtg gccaacgacc
     2461 gaacctgttc ggagatggcg tcggcgtcgc gcaggcggat cggtaccggg agcggcacgg
     2521 ccaccgcgcc ggccgaccat acggagaagg cccagcgggc ccagtgatgg tcgttcgacc
     2581 cgaggatccc gacccggtcg cccttaccga ccccgcggtc ggcgagcagg gcgcgcccgg
     2641 tgccgcaggt cgagagcagc tcggagatcg tgaaggagac cggcccatcc tccaggtaga
     2701 agtgggccgt cgcgtcgacg ttcgtcgcgg cggtctccag gccctcgtgg atggagttgg
     2761 ccgtgaggag ggtgtcgatc cgctcttcga ccgggatgat caccgtgaca cggtctggcc
     2821 gcggcgtttg gcctcgacga ggttcacgac tgcgttgatc gactcgaagt tctcctcgac
     2881 gagctcgtcg tcggtgaacg tgacgttgaa ctccttctcc aggaacacca cgagctgctc
     2941 gaccgccagc gagtcgagga gtcccatcgc cagcggatcc ccgtcgaccg aggcgccctc
     3001 gaggagctcc cggccgacga acttctcgat cttcgagcgc gtcctcatcc gtcctcgttc
     3061 ctcctgccgc ggtcgtcacc gatggggcgc cgccacccgt tggtgggcga ggggggactt
     3121 gaacccccat gaggttgccc tcacaggatc ctgaatcctg cgcgtctacc aattccgcca
     3181 cccgcccagg atcgaccatt gtacagatgc gggcgctcgc cctcccgccc ctccgcgttt
     3241 gggcctgaac tgccgttggt atgatccccg agcgccctcc cgagggcagg cgcgcacgat
     3301 cgcccgagcg tatggccaac ctccgcgact tcgagcgacg cctgggagac ctggtcgagg
     3361 gcttcttcgg caaggccttc cgctccggcc tgcagcccgt cgagctcggg cggcggatcc
     3421 tccgggagat ggaggccggg cgcacggtcg gcgtccagaa cgtctgggtg ccgaaccact
     3481 acacgttcca cgtcgccgcc gaggaccgcg accacttctc ccaggtcgag caggccatcg
     3541 gcaaggagct cgagggcgtg gtgctggaga acgcccgtga gcgaggctgg ggcctgctcg
     3601 gcccgcccga agtggtgttc gagaccgacc cggacctgaa gaagggcacc ttccactgcg
     3661 aggcgcgcct ggtgcaggcc gaggacgtgc gcaccggcga gcacgcggcg tacctgcccg
     3721 cggcaggggc tggggctccg gcggcggcgg gcccggccgc cctcgtgctc atcgccggcg
     3781 gttccgagag cgggaggttc ccgctcgacc gcgacgtggt acgtatcggt cggatggcag
     3841 actgcgacgt cgtgctggag gactccgggg tcagccgcca ccacgccgag gtccggcggg
     3901 aggacgggca cttcgtcatc gccgacctcg gctccacgaa cgggacgatg gtgaacgagg
     3961 cgcaggtgtc ggagcgcgag ctcgaggagg gcgaccgcat caccatcggg cgttcggtgc
     4021 tcgagttccg ccgggggtag gcccgtgccg cccttcgtcc tgacggtcct gaagttcgcg
     4081 ttcctcgcgc tcctctacta cttcgtctac cgcgcgatcc gcgtggcggt ggtcgcggtc
     4141 ggggagcggc gtcccgggcg caagaagggc gcgcctcccg cgccgcgacc gaaggcccgg
     4201 cgcggcgggc gggagccggg cgccgtcgtc ctgctggcgc cggacgggaa gaagcagcgg
     4261 tcattccggc tgaccgggac ggtgcagatc ggacgggccg aggcctgcca gatccggctg
     4321 gacgacacgt acgtctcaca gttccacgcc cggctgttca gccgcaacgg cgcgtggttc
     4381 gtcgaggacc tcggctcgac gaacgggacg tacctcaacc agcagaaggt gaccgcgccg
     4441 accccggtgt ccctcggtga ccggctcaag gtgggcaagg ccgtcctgga gctccgaagg
     4501 tgaagctctc cgtcggggcg cagagcgacg tgggccgtgt ccgggagcgc aacgaggacg
     4561 cgctgctgct gcacgacccg ctctacgcgg tggccgacgg gatgggcggc caccgcgccg
     4621 gggagatcgc ggcgcgcacg gcggtcgaca ccctccgcgg tgagccgctc ggcgacggcg
     4681 cgcacgcgct ggcggagcgg gtgcgccagg cgaaccgggc ggtcctcgac accgccgccg
     4741 gcgaccgggc gctggagggc atggggacga ccctcaccgc cgtcctgctc gccggcgacg
     4801 agctgcgcat cgcgcacgtc ggcgactccc gcgcctacct gctgcgcggg gacgcgttca
     4861 cccagatcac cgaggaccac acgctcgtgc agcggatggt ccggcagggc cggctgacgc
     4921 cggcggaggc cgaggtccat ccgcagcgca gcatcctgac ccaggccctc ggcgtagacc
     4981 ctgctatcga ggtggacgag ctgtcggtcc cggtcctgcc cggcgaccgg gtgctgctct
     5041 gcaccgacgg cctgaccggg atgctctccc aggaggagat cgaggggacg ctgcgcgaga
     5101 cccccgaccc gcaggcggcc gccgacgcgc tggtggcggc cgccgtggac gccggcggga
     5161 tcgacaacgt cacggtgctg gtgatcgacc tcctggacga ctccggaggc cggccggcgc
     5221 tgccgcccgc gcccgaggag gccgacgagg ccgaggaggc cgaggaggcc gaaccggagg
     5281 gcgagaccgg ggacgagccg gacgacaccg cgtcggtcga gcgggtcgcc cgcccggccc
     5341 gggcccccgc ccggccgcgg cggtggcggc gggcggtcgt ggcggccgcg gcggtggcgc
     5401 tgctggtgac cggcgccgtc gccgccaagg cgctctacgt ggaccgccag tggtacgtcg
     5461 gggagagcaa cgggatggtc gccatcttcc gggggctgcc cgccgaaccg ctcgggctcg
     5521 acctgtccac cctcgtcgag gagaccggcc tcccggccgc ggaggtcgag cggctg
//
LOCUS       DASPWO010000002         4715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_85147, whole genome shotgun sequence.
ACCESSION   DASPWO010000002 DASPWO010000000
VERSION     DASPWO010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85147"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1751
                     /locus_tag="VEO00_00040"
     CDS             <1..1751
                     /locus_tag="VEO00_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYU56423.1"
                     /translation="RDYTRRSDIPRLFFTERHGYTVVASPRSARFAHAREAAWRGAMD
                     AGVFEGAPTYDSAAAAATVEYAVAIRAPRDSEPVGVFQATLALDKLAGLLAVADLGDS
                     AYLQVVDGGGRLLVTPDPAQLLQPLAEGEAIPRREQPVDAVVATARGPELVVSAPANA
                     GKWWVLLRQPASLAYAPADRTERTIRIGAGAALLATIVFLGLLAVWLDRRVTGPVKAA
                     GAIASRVAGGDLSVTVATQRADSAEVGELLSAVHSMVAALRRLVGAIRAAADEAAAMA
                     TEISASTEEMSASTQEMSATCQDLTRRAAEQTELVGAATADAGKILEMATILAAGAQD
                     SVRRNTAVADVARTNREVLDHSTAQLAKLAEEVERGAQEAGALTKAAAEIQKFVTQTK
                     AIATQTNMLALNAAIEAARAGPQGRGFAVVADEVRKLASVAAAAAGDTADTVRGVLGR
                     IEATRERLAQLAKGGAAARDAAQTAAQGLATVVAEAQASDIWSREIAKMAEEMQALVE
                     EISTRLGSVAQGTESLLASAQEIAASSQQQSASTQEIAASANQLAEASDRLTAAVKSF
                     RLLAEEQATPARQAAD"
     gene            complement(1748..3109)
                     /locus_tag="VEO00_00045"
     CDS             complement(1748..3109)
                     /locus_tag="VEO00_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYU56424.1"
                     /translation="MIKVACVLAPCLAAGLAAQQPFAIPDAVRANVRARVDGGWAPGI
                     VVGVVDSSGTRYFAYGRTAVNGGQPVDERTVYEIGSITKAFTGVALADMAVKGEVALD
                     DPVRRYLPDSVRVPGTDSQPISLRLLSAQRSGLPRMPTNFSPRDSSNPYADYDAARLY
                     AFLNGYALTRAPGAAYEYSNLGFGLLGLALARRAGTTYEDLVRRRILAPLGMTSTMIR
                     LTDAARARLAQGSADGRPAANWDLDAFAGAGALRSTAEDLTKFLAAALGLRGTSLDSA
                     FRLAAEPQFDAGPGGGMRIGLGWHVREGAGGARIVWHNGGTGGYHSWAGYDPARRVGV
                     VVLTNSAENIDDIGLHLLDPPHPLAPVRTAVALPAAALDEYLGRYPLLPTFVITVTRD
                     GDHLMAQATGQPAFRIWASAPDEFFLKVVDAQLGFTRGADGKVNGLILHQNGRDQRAE
                     RAP"
     gene            complement(3106..>4715)
                     /locus_tag="VEO00_00050"
     CDS             complement(3106..>4715)
                     /locus_tag="VEO00_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56425.1"
                     /translation="DGRQLFVTAMVERREEILRRPFDQLRMEVIAGAGQGEQGTGNSR
                     PFVSPDGRWIAYAKQGKLRKVPVDGGPAIDLAVADWAGGSWGKNGRLVYTRAYNTGLW
                     IVSEGGGDARVLTTPDTAQGELGHWWPQILPDGEHVLFTAYRTPIERATIEVLSISSG
                     RRDVLVTGGVFGFYVPTGHVLYAVGETIRAVPFDLGRLAVTGSALPVVDSVAMNPTDG
                     AAAFDVSENGTLAYLPVSSYVTETEVVLVDRQGKESRALPATDRYNHPRLSPEGSRIA
                     VDIRSANSLGDVWMFQVGRSGGTRVTAEGGRDFGAEWTPDGRELIYSSEHPFFDLYRR
                     AADASRPAEPLLTGTYDRYTGSVSQDGRLFAFVLSVPGGAELWTVQLRGPPAPARYFA
                     NGFNLAHPTLSPDGRWMAYDSDESGRVEVYAQSFPNPSLRRWKVSPANGSEPMWTRVG
                     RELVYRKGDSVMAVTVDLENGRSGPPRALFGGRYPDSPGWTRPRSYDVSRDGERFLMT
                     KLPAEQPRPRIAVVLNWFAELRAKVPP"
BASE COUNT          698 a         1652 c         1748 g          617 t
ORIGIN      
        1 tgcgcgacta cacacggcgc tccgatatcc cccggctgtt tttcacggag cgccacggct
       61 acacggtggt ggcgtcgccc cgctcggcgc gcttcgcgca cgcgcgcgaa gcggcctggc
      121 ggggggcgat ggacgcgggc gtgttcgagg gggcgccgac gtacgactcc gcggcggccg
      181 ccgcgaccgt cgagtacgcc gtggccatcc gggcgccgcg cgactccgag ccggtcgggg
      241 tgttccaggc gacgctggcg ctcgacaagc tcgcggggct gctcgcggtc gccgacctgg
      301 gggacagcgc ctacctccag gtcgtggacg gcggcggccg cctcctggtg acgccggacc
      361 cggcgcaact gctccagccg ctcgcggagg gcgaggcgat cccccggcgg gagcagccgg
      421 tcgacgccgt cgtggcgacg gcgcgcgggc cggagctcgt cgtgtccgcc ccggccaacg
      481 cgggcaagtg gtgggtgctg ctccggcagc cggcgtctct ggcctatgca ccggcggacc
      541 gcaccgagcg cacgatccga atcggcgcgg gggcggcgct cctcgccacc atcgttttcc
      601 tggggctcct ggccgtgtgg ctcgaccggc gggtgaccgg accggtgaag gccgcggggg
      661 cgatcgcgag ccgggtggcg gggggcgatc tgtcggtcac agtggccacg cagcgggccg
      721 actcggccga agtcggagag ctgctttccg ccgtgcattc gatggtcgcg gcgctgcgcc
      781 ggctggtggg ggcgatccgc gccgcggcgg acgaggcggc ggcgatggcg accgagatct
      841 cggcctccac cgaggagatg agcgcttcga cccaggagat gtcggccacc tgccaggatc
      901 tcaccaggcg cgcggcggag cagaccgagc tggtgggcgc cgccacggcc gacgcgggga
      961 agatcctgga gatggcgacg atcctcgccg cgggcgccca ggactcggtg cgccgcaaca
     1021 ccgcggtggc ggatgtcgcg cgaaccaaca gagaagtgct ggaccacagc acggcccagc
     1081 tcgcgaagct cgccgaggaa gtggagcgcg gcgcgcagga ggcgggcgcg ctcaccaagg
     1141 ccgcggccga gattcagaaa ttcgtcaccc agaccaaggc gatcgcgacc cagaccaaca
     1201 tgctggcgtt gaacgccgcg atcgaggcgg cgcgggcggg cccgcagggc cggggcttcg
     1261 ccgtggtggc ggacgaggtg cgcaagctcg cctcggtggc ggctgcggcg gccggcgaca
     1321 cggcggacac cgtgcgcggc gtgctcgggc ggatcgaggc gacgcgggag cggctggcgc
     1381 agctcgcgaa gggcggcgcg gcggcgcgcg atgcggccca gacggcggcg cagggcctcg
     1441 cgaccgtcgt cgccgaggcc caggcgagcg acatctggag ccgcgagatc gcgaagatgg
     1501 ccgaggagat gcaggcgctc gtggaggaga tcagcacccg gcttggctcc gtggcccagg
     1561 gcaccgagag cctgctggcc tccgcccagg agatcgcggc gtccagccag cagcagagcg
     1621 cctccaccca ggagatcgct gcgtcggcca accagctggc cgaagcgtcg gaccggctca
     1681 cggccgcggt gaagtcgttc cggctactgg cggaagaaca agcgacaccc gcccggcagg
     1741 cagcggacta aggcgcccgc tcggcgcgct ggtcgcggcc gttctggtgc aggatcagtc
     1801 cgttcacctt gccgtcggcg ccgcgcgtga agccgagctg cgcgtccacc accttcagga
     1861 agaactcatc gggagcgctg gcccagatcc ggaaggcggg ctgtccggtg gcctgcgcca
     1921 tcaagtgatc gccgtcgcgc gtcacggtga tgacgaacgt cggcagcagc gggtatcgcc
     1981 ccaggtactc gtcgagggca gccgccggca gggcgaccgc ggtgcgcacc ggcgcgagcg
     2041 ggtgcggggg atcgaggagg tgcagcccga tgtcgtcgat gttctcagca gagttcgtga
     2101 gcaccacgac gccgacgcgg cgggccggat cgtaccccgc ccagctgtgg tagccgccgg
     2161 tgccgccatt gtgccagacg atgcgcgccc ctccggcgcc ctcgcgcacg tgccagccca
     2221 ggccgatgcg catgccgcct cccggtcccg cgtcgaactg cggctcggcc gccagccgga
     2281 acgccgaatc gagcgacgtg ccgcgcaggc ccagcgcggc ggcgaggaac ttcgtcagat
     2341 cctcggcggt ggaacggagc gcgccggcgc cggcaaaggc gtccaggtcc caattggccg
     2401 cgggccggcc gtcggcggag ccctgggcga ggcgggcgcg cgccgcgtcg gtgagcctga
     2461 tcatcgtgct cgtcatcccg agcggggcca ggatgcggcg gcggaccagg tcttcgtagg
     2521 tggtgccggc gcggcgcgcc agcgccaggc ccagcaggcc gaatccgagg ttcgagtact
     2581 cataggcggc gccgggtgcg cgcgtcagcg cgtagccgtt caggaacgcg tacagccgcg
     2641 ccgcgtcgta gtcggcatag ggattgctgg agtcgcgcgg cgagaaattg gtcggcatac
     2701 gcggcaggcc cgagcgctgg gccgagagca accgcaggct gatgggctgc gagtcggtcc
     2761 ccggcacgcg taccgagtcg gggaggtagc gacggacggg atcgtcgagc gcgacctcgc
     2821 ccttcacggc catgtcggcc agggccacgc cggtgaacgc cttggtgatc gagccgatct
     2881 cgtagacggt gcgttcgtcg acgggctggc cgccgttcac ggccgtcctg ccgtaggcga
     2941 agtagcgggt gccgctcgag tccacgacgc ccacgacgat gcctggcgcc cagcccccgt
     3001 ccacacgcgc gcgcacgttg gcgcgcacgg cgtcggggat ggcgaagggt tgttgggcgg
     3061 ccaggccggc ggcgaggcac ggcgcgagga cgcaggcaac cttaatcaag gcggcacctt
     3121 cgcccggagc tcagcgaacc agttcagcac caccgcgatc cgcggccggg gctgctcggc
     3181 cggcaacttg gtcatcagga accgctcgcc gtcgcgcgag acgtcgtagc tccgaggccg
     3241 ggtccacccg gggctgtcgg gataccgccc gccaaacagc gccctgggcg ggccgctccg
     3301 gccgttctcc aggtcgacgg tcaccgccat cacgctgtca cccttccggt acaccagctc
     3361 ccgcccgacc cgggtccaca tcggctccga cccgttggcc ggcgagacct tccatcgcct
     3421 caggctcggg ttgggaaacg actgggcgta cacctcgacg cgacccgatt catcggaatc
     3481 gtacgccatc caccggccat ccggcgacag ggtgggatgc gcgaggttga agccgttggc
     3541 gaaatagcgc gccggcgccg gcgggccacg aagctgaacg gtccacagct ccgcgccacc
     3601 cgggacgctg agcacgaagg caaacagtcg cccatcctgg gagacggaac cggtgtagcg
     3661 gtcgtacgtg ccggtgagca gcggttcggc gggccggctc gcatcggccg cccggcgata
     3721 gaggtcgaag aacgggtgct cgctcgagta aatcagctcc cgaccgtcgg gcgtccactc
     3781 ggcgccgaaa tcccggccgc cttccgctgt gactctcgta ccgcccgagc gcccgacctg
     3841 gaacatccag acgtccccca gggagttcgc cgagcggatg tccacggcga tccggctccc
     3901 ctctggtgag aggcgcgggt ggttgtagcg atcggtcgcg ggaagggccc gcgactcctt
     3961 gccctgccgg tccaccagca ccacttcggt ctcggtgacg taggagctga ccggcagata
     4021 cgccagggtc ccattctccg acacgtcgaa ggccgccgct ccgtccgtgg ggttcatcgc
     4081 cacgctatcc acgacgggga gggcggagcc ggtcaccgcg aggcggccaa ggtcgaacgg
     4141 gacggctcgg atggtctcac ccaccgcata gagcacgtgt cccgtgggca cgtagaagcc
     4201 gaagacgccc ccggtgacca gcacgtcccg cctgccggag ctgatcgaga ggacctcgat
     4261 ggtggctcgc tcgatcggcg tgcggtacgc ggtgaacagg acgtgctcgc cgtcgggcag
     4321 gatctgcggc caccagtgac ccagctcgcc ctgcgccgtg tccggagtcg tgagcacgcg
     4381 cgcgtcgccc ccgccctcac tcacgatcca gagaccggtg ttgtaggccc gggtatacac
     4441 gagccggccg ttcttgcccc aactgccgcc ggcccagtcg gccacggcga ggtcgatcgc
     4501 gggcccgcca tccaccggga cctttcggag cttcccctgt ttggcgtagg cgatccaccg
     4561 tccgtccggc gaaacgaacg gccggctgtt gccggtcccc tgctctcctt ggccggcacc
     4621 cgcgatcact tccatccgca gctggtcgaa cggacgacgc agaatctctt cccgccgctc
     4681 gaccatggcg gtgacgaaga gctgtcggcc gtcgg
//
LOCUS       DASPWO010000003         4078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_594756, whole genome shotgun sequence.
ACCESSION   DASPWO010000003 DASPWO010000000
VERSION     DASPWO010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594756"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VEO00_00055"
     CDS             complement(<1..295)
                     /locus_tag="VEO00_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56426.1"
                     /translation="MSRSRIVLATVLCLLAAGIFVGIGAVPASAGAPSYCEEEFTGTS
                     GDDVIVGDDGDNHICGRAGNDKLFGLEGDDTLEGQDGNDQLFSGDDWDWDVLLG"
     gene            complement(468..1127)
                     /locus_tag="VEO00_00060"
     CDS             complement(468..1127)
                     /locus_tag="VEO00_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56427.1"
                     /translation="MPASAGVGPDPTQPCDPEFTGTSGDDVIFGSDWDDVICGRAGND
                     KLFGGDGEDVLKGQDGNDQLFGGDDSDSLVGGIGGDRLVAGFGVDLLNGGPGKDTADY
                     SLTCGVIGSLNTGFIDNCADDDYVTFANGVSTVENLIGSPVFNDELEGDGQANEIRGN
                     GGNDFISGRGGEDRLFGEAGDDLIRGDGGDDFINGGDQSTIGDVCSGGAGTNTIINCF
                     P"
     gene            complement(1372..2112)
                     /locus_tag="VEO00_00065"
     CDS             complement(1372..2112)
                     /locus_tag="VEO00_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56428.1"
                     /translation="MRGRIALAAVLCLLAAGIFVGMGALPASAGGPPDPTQPCDPEFT
                     GTSGDDVIFGDDGDNVLCGRAGNDKLFAGEGFDVLKGQDGNDQLFSGDDWAWDYLLGG
                     RGADRLVPVDDTNTLNGGPGHDEADYSFNDCAIDAFLFDGVIYKGKCGTDSLDFGGGL
                     STVEDVTGTVWFDSIEGDVSANVLMGGASDDEIHGRGGEDSLYGQLGDDALFGEGGND
                     YLNGGDQSTVGDVCFGGAGTNTIVNCFP"
     gene            complement(2153..2887)
                     /locus_tag="VEO00_00070"
     CDS             complement(2153..2887)
                     /locus_tag="VEO00_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56429.1"
                     /translation="MRGRIALAAVLCLAAGLIVGIGALPASAGANPTCDPEFTGTSGD
                     DVIVGDGYDNHICGRAGNDKLFGLEGEDVLEGQDGNDQLFAGDDYDYDYLWGGRGRDR
                     LVPGWDYNELNGGPGHDVADYSIGTEGCNVQAYLWDAYIDKCDGYYYDSIVFANDLST
                     VENVTGTPWEDYFEGDVQANRLVGGASGDEAHGRGGEDSLYGQLGDDLLLGEGGNDYL
                     NGGDQSTVGDVCFGGAGTNTIVNCFP"
     gene            complement(3014..3232)
                     /locus_tag="VEO00_00075"
     CDS             complement(3014..3232)
                     /locus_tag="VEO00_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HYU56430.1"
                     /translation="MDYLEAAVAVLRDAGEPLHWTVIQDRALKAGYLDPFTQRDIRRN
                     LLAALAAAVREGIVEKRETGVYTLAEGA"
     gene            complement(3232..>4078)
                     /locus_tag="VEO00_00080"
     CDS             complement(3232..>4078)
                     /locus_tag="VEO00_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYU56431.1"
                     /translation="QDALGPYGRDELDAITVRYTPRVTDQMPSAPADAHLGGAAWSFY
                     VTFDHRRTVTADRSFRLALAHAVDRLALAEALPANLILATGGVVPPALQGHTPDIVPR
                     FDPDRAREYLRRSGFDGTIELLDVEERASLSRIVVEGWRDTLGIDLRARVLSLPEVMA
                     LRDPWSVAPLVVSGWLPGYPDPEYFLRLLLHSESKTNEGGFADPAFDALIERARQERD
                     DRARLELFHQADRMAVAEQVALLPLAYGRSMAFVKPWVDGWWEFGKSSASFADLTIDP
                     SSPRA"
BASE COUNT          578 a         1513 c         1345 g          642 t
ORIGIN      
        1 cacccagcag gacgtcccag tcccagtcgt cgccggagaa cagctggtcg ttcccgtcct
       61 ggccctcgag ggtgtcatcg ccctccaggc cgaacagctt gtcgttcccg gcccggccgc
      121 agatgtggtt gtcgccgtcg tcgccgacga tgacgtcgtc cccgctggtg ccggtgaact
      181 cctcctcgca gtatgacggc gcgccggccg aagccggcac cgcgccgatc ccgacgaaga
      241 tccccgccgc cagcagacag agcacggtgg cgaggacgat gcgcgatcga ctcactagtt
      301 gccccctttc ccggtgtcgg ctcccgccgc cgaaactcgc ggccgccgac gctggcggga
      361 gcatcgcgcc tgtccgtgct tccgtcaaga ccggacgcag gagggccgga ggaatcctcc
      421 ggccctcgac tcgttggtcc cggttccctt cgtccgggtg cgcccgccta cgggaagcag
      481 ttgatgatcg tgttcgtgcc cgccccgccg gagcagacgt cgccgatggt cgactggtcg
      541 ccaccgttga tgaagtcgtc gccgccgtct ccccggatca ggtcgtctcc ggcctctccg
      601 aacagccggt cctcgccgcc gcgcccactg atgaagtcgt taccgccgtt gccccggatc
      661 tcgttggcct gcccgtcacc ctccagttcg tcgttgaaga ccggggagcc gatcaggttc
      721 tccaccgtgc tgacgccgtt ggcaaacgtg acgtagtcgt cgtcggcgca gttgtcgatg
      781 aatcccgtgt tcagcgatcc gatcacgccg caggtgaggc tgtagtccgc cgtgtccttg
      841 cccggacccc cgttcaggag atcgacgccg aagccggcca ccaggcggtc gcccccgatc
      901 ccgcccacca gcgagtcgct gtcgtcgccg ccgaagagct ggtcgttgcc gtcctgtccc
      961 ttcaggacgt cctccccgtc accgccgaac agcttgtcgt tcccggcccg gccgcagatg
     1021 acgtcatccc agtcgctgcc gaagatcacg tcgtcgccgc tcgttccggt gaactccggg
     1081 tcacagggct gggtgggatc cggtccgacc ccggccgagg cgggcaaagc cgtcaaccca
     1141 acgagcatgc ccgccgtcag aaggcacagc gctgccgcaa gaatgatccg agatcgaccc
     1201 acgaggcttc ccctttcccg gcttggccat cgaggcggcc ggcacctccg gtcgccccct
     1261 cagctgcagc atcgtgtgcg gcgacggccg agtcaagagg cccgaacgcc cgagggccgg
     1321 ggggtccctc ccggccctcg cagcgcgtct cggagaccgc gacccgtcgc tctacgggaa
     1381 gcagttgacg atcgtgttgg tcccggcgcc gccgaagcag acgtcgccga ctgtcgactg
     1441 atcgcccccg ttcaggtagt cgttcccgcc ctcgccgaag agcgcgtcgt cgccgagctg
     1501 cccgtagagc gagtcctcgc caccgcggcc gtggatctcg tcgtcggagg cgccgcccat
     1561 caggacattc gcggagacgt ccccctcgat ggagtcgaac cagaccgtgc cggtcacgtc
     1621 ctccaccgtg ctcagcccgc ccccgaagtc gagggagtcc gtgccacact tgcccttgta
     1681 gatgaccccg tcgaacagga aggcgtcgat ggcgcagtcg ttgaacgagt agtcggcctc
     1741 gtcgtggccg ggaccgccgt tcagggtgtt cgtgtcatcg acggggacca gccggtccgc
     1801 gcccctgcca cccagcaggt agtcccaggc ccagtcgtcg cccgagaaga gctggtcgtt
     1861 gccgtcctgg cccttcagga cgtcgaagcc ctccccggcg aacagcttgt cgttcccggc
     1921 gcggccacag aggacgttgt caccgtcgtc gccgaagatg acgtcgtctc cgctcgtgcc
     1981 ggtgaactcc gggtcacaag gctgggtggg gtccgggggt ccgccggcgg acgccggaag
     2041 cgcccccatc ccgacgaaga tccccgccgc cagcaggcag agcaccgccg ccagcgcgat
     2101 gcgccctctc atgtgcatct tcctctctcc cgggcttcgg cccggctcgt tcctacggga
     2161 agcagttgac gatcgtgttg gtcccggcgc cgccgaagca gacgtcgccg accgtcgact
     2221 ggtcgccgcc gttcaggtag tcgttcccgc cctcgcccag caggaggtcg tccccgagct
     2281 ggccgtacag cgagtcctcg ccgccgcgcc catgggcctc gtcgccggac gccccgccca
     2341 cgaggcggtt ggcctgcacg tccccctcga agtagtcctc ccagggagtc ccggtgacgt
     2401 tctcgaccgt gctcaggtcg ttggcaaaga cgatcgagtc gtagtagtag ccgtcgcact
     2461 tgtcgatgta cgcgtcccac aggtaggcct ggacgttgca cccctcggtg ccgatgctgt
     2521 agtcggccac gtcgtgcccc ggaccgccgt tcagctcgtt gtagtcccag cccggaacca
     2581 gccggtcgcg cccgcgcccg ccccagaggt agtcgtagtc gtagtcgtct ccggcgaaca
     2641 gctggtcgtt gccgtcctgg ccttccagga cgtcctcgcc ctccaggccg aacagcttgt
     2701 cgttcccggc ccggccgcag atgtggttgt cgtacccgtc gccgacgatg acgtcgtcac
     2761 cgctcgtgcc ggtgaactcc gggtcgcaag tcggattggc ccccgccgag gccggcaacg
     2821 cgccgatccc cacgatgaga cccgccgcca ggcacagcac ggccgcgagc gcgatccgcc
     2881 ctctcatccg catcctcctc tctcccgggc ttccggcccg gttcgttccc tacgggtgcg
     2941 cttctgtttc cgcgttcgcc ggacagcggg gagcatggac gaaacggccg atagggtcaa
     3001 tggcccgccg tgactaggcg ccctcggcca gcgtgtagac cccggtctcg cgcttctcga
     3061 cgatcccctc ccggaccgcc gcggcgagcg cggcgagcag gttccggcgg atgtcgcgct
     3121 gggtgaacgg gtcgaggtag ccggccttca gcgcgcggtc ctggatgacc gtccagtgca
     3181 ggggctcgcc ggcgtcgcgc agcaccgcca ccgcggcctc gaggtagtcc atcaggcccg
     3241 cggcgacgac gggtcgatgg tcaggtcggc gaacgacgcg gaggacttcc cgaactccca
     3301 ccagccgtcg acccacggct tgacgaaggc catgctccgc ccgtaggcca gcgggagcag
     3361 cgcgacctgc tcggcgacgg ccatccggtc ggcctgatgg aacagctcca ggcgggcgcg
     3421 gtcgtcgcgc tcttggcgcg cgcgctcgat cagggcgtcg aaggccgggt cggcgaaccc
     3481 cccctcgttc gtcttgctct cggagtggag cagcaggcgc aggaagtact ccgggtccgg
     3541 gtagcccggc agccaaccgg acaccacgag cggcgccacc gaccacgggt cccgcagcgc
     3601 catgacctcg ggcagggaga gcacccgcgc ccgcaggtcg atcccgagcg tgtcccgcca
     3661 cccctcgacc acgatccggc tgagcgaggc gcgctcctcc acgtcgagca gctcgatcgt
     3721 cccatcgaag ccgctgcggc gcaggtactc gcgcgcgcgg tccggatcga agcgcgggac
     3781 gatgtcggga gtgtggccct gcagcgccgg cgggaccacg ccgccggtgg cgaggatcag
     3841 gttggccggc agcgcctcgg ccagcgctag gcgatcgacg gcatgggcca gcgccaggcg
     3901 gaacgagcgg tcggcggtga ccgtccgccg gtgatcgaac gtgacgtaga agctccacgc
     3961 cgcccccccg aggtgggcgt ccgccggggc cgagggcatc tggtcggtga cccggggcgt
     4021 gtagcggacc gtgatcgcgt cgagctcatc gcgcccgtag ggcccgagag cgtcctgc
//
LOCUS       DASPWO010000004         2887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1188769, whole genome shotgun sequence.
ACCESSION   DASPWO010000004 DASPWO010000000
VERSION     DASPWO010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188769"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /locus_tag="VEO00_00085"
     CDS             <1..1891
                     /locus_tag="VEO00_00085"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HYU56432.1"
                     /translation="YRLPPSGFVQGTVKDANDNLPVAGATVRARQGANVVRSTTTDAS
                     GFYRLQLRLDSYTIEATATNYSTGSAAVVLDTEDEVVTQNFTLRTARGVVSPTSLQFI
                     VPPNQTRSKMLTLRNTGSLDMTWQIRETGGAAPQQTPSPGEAISGEKNKGYDPNARTT
                     EGLYVNGTPAGWPASAPGDVIRSWPPTGLSLAWGVGYTGNVWLSDVPTNNRNHEFTVL
                     GAATGRNWPAPWAGAWPGDMAYDAGRGIMCQVNVGGDNGIYCWNPNTGAVVDSITGAF
                     PWTTISQRGLAYRPDDDTFYIGGWNQFILYHVKGLSYPDKGAVISQCNPPDGNISGLA
                     WNPAFNIVWEATNTPTDTIYELNPDTCAVLATLPHPNPGFSGAGLEMDEAGNLWMIDQ
                     SPNTVYLIESGVPAFVDVPWLSESPTSGTLAPGGTQNVQVTVNTTGLTPGVYNATLFI
                     QTNSGRQPTLRVAVNLIVPAYRQGVDVAAVGGYIDGNSDTWAPDQAYTAGSWGYIYRG
                     NEASTRHAIAGTDDDALYQTARRGQTEYRFDGLPSGVYQVELRFAEILNRKPNQRLFD
                     VLIEGNLVLYAFDIAGEVGQDTADDKSFFVPVTDGQMNIRFITRQADKEPLFNAIRVT
                     HRPDR"
     gene            complement(2036..>2887)
                     /locus_tag="VEO00_00090"
     CDS             complement(2036..>2887)
                     /locus_tag="VEO00_00090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU56433.1"
                     /translation="PAGLVFRDMTPEDVPAVLRLCRLAGWNQRTEDWQPLLANGFFRV
                     AVRDATVVGSGGAVVYGRRLAWVAMILVDPDARRQGIATRLMRDALARLKAVEVVGLE
                     ATPQGAPVYGGLGFTDRGGFARMERPADAEPVLAEQGPTVPRPLTAPDLVGLAARDGA
                     VFGADRSAVLAWAREQAPEYAWCIGAREKSEGYVLGRHGHRTEHLGPLVADDEETAAR
                     LLTAILAAVPGRAFTIDIPDRPAWRERLRILGFREQRPFRRMILGDAPSPGDPRQRFA
                     ILGPEFG"
BASE COUNT          499 a         1068 c          963 g          357 t
ORIGIN      
        1 ctaccggctg ccgcccagcg gcttcgtgca gggcacggta aaggacgcca acgacaacct
       61 accggtggcc ggggcaaccg tccgcgctcg ccagggcgcc aacgtcgtcc gctcgacgac
      121 caccgacgcc tccgggttct accggctgca gctccgcctc gacagctaca cgatcgaggc
      181 caccgccacg aactacagca ccgggtccgc cgcggtggtc ctggacaccg aggacgaggt
      241 cgtgacccag aacttcacgc tccgcaccgc gcggggcgtg gtgagcccga cgtcactcca
      301 gttcatcgtc ccgccgaacc agacgcggag caagatgctg acgctgcgca acaccggcag
      361 cctcgacatg acgtggcaga tacgggagac tggcggcgcc gccccgcagc agacgccgtc
      421 ccctggcgag gcgatctccg gcgagaagaa caagggctac gaccccaacg ccaggaccac
      481 ggagggactg tacgtcaacg gcacgccggc cggatggccg gcgtcggctc cgggcgacgt
      541 catccgctcg tggccaccga cggggctgtc gctggcctgg ggtgtcggct acacgggcaa
      601 cgtctggctc tcggacgtcc cgaccaacaa ccggaaccac gagttcaccg ttctgggcgc
      661 ggcgaccggc cgcaactggc cggcgccatg ggcgggggcc tggccgggcg acatggcgta
      721 cgacgccgga cgcggcatca tgtgccaggt caacgtgggc ggagacaacg ggatctactg
      781 ctggaacccg aacaccggcg ccgtcgtcga ctccatcacg ggagccttcc cgtggacgac
      841 catctcccag cgtggcctgg cctaccggcc cgacgacgac acgttctaca tcggcggctg
      901 gaaccagttc atcctgtacc acgtgaaggg gctctcctat cccgacaagg gagccgtgat
      961 cagccagtgc aacccgccgg acggcaacat ctccggcctc gcctggaacc ccgccttcaa
     1021 catcgtctgg gaggcgacga acaccccgac cgacaccatc tacgagctca acccggacac
     1081 ctgcgcggtg ctcgcgaccc tgccgcaccc gaacccgggc ttcagcggcg caggtctcga
     1141 gatggacgag gccgggaacc tctggatgat cgaccagtcg ccgaacacgg tctacctgat
     1201 cgagagtggc gtgccggcct tcgtggacgt gccgtggctg tccgagagtc ccacgagcgg
     1261 cacgcttgct ccgggcggaa cgcagaacgt ccaggtgacg gtcaacacca ccgggctcac
     1321 acccggcgtc tacaacgcga cgctgttcat ccagacgaac agcggccggc agccgacact
     1381 gagggtggcc gtcaacctca tcgtcccggc ctaccggcag ggcgtcgacg tcgcggccgt
     1441 tggcggctac atcgacggga acagcgacac ctgggcgccc gaccaggcgt acaccgcggg
     1501 atcctggggc tacatctatc gcggcaacga ggccagcacg cgccacgcga tcgcgggcac
     1561 cgatgacgac gcgctctacc agacggcgcg ccggggccag accgagtacc gcttcgacgg
     1621 gctgccgtca ggcgtgtacc aggtcgagct ccggttcgcg gagatcctga accggaagcc
     1681 gaaccagcgg ctcttcgacg ttctcatcga gggcaacctc gtcctgtacg cgttcgacat
     1741 cgcaggcgag gtcgggcagg acaccgcgga cgacaagagc ttcttcgtcc cggtgacgga
     1801 cggccagatg aacatccgct tcatcacgcg gcaggccgac aaggagccgc tcttcaacgc
     1861 catccgcgtg actcaccgcc cggaccgcta gccgccgacc gggctccacc aaggacggcg
     1921 gccccgcgaa agcggggccg tcgtccctca ttcccgaaaa tgcccctgac ggtgagggga
     1981 caagccccct ccgagcgggc gttcctgtgt ccgagacccc tccgggaggg tctgatcagc
     2041 cgaactcggg gccgaggatg gcaaaacgct gccgggggtc gcccgggctc ggcgcatccc
     2101 ccaggatcat ccggcggaac ggccgctgct cgcggaagcc gaggatgcga aggcgctccc
     2161 gccacgcggg gcggtcgggg atgtcgatgg tgaaggcacg gccgggaacc gccgcgagga
     2221 tcgcggtcag caggcgcgcc gccgtctcct cgtcgtcggc caccagcgga ccgaggtgct
     2281 cggtgcggtg cccgtgacgc cccaggacgt aaccctcgga tttctcccgc gcgccgatgc
     2341 accaagcgta ttcgggagcc tgctcgcgcg cccaggccag gaccgccgaa cgatcggcgc
     2401 cgaacacggc cccgtcccgg gcggccaacc caacgaggtc gggagccgtg agcgggcgcg
     2461 gcacggtcgg cccctgctcc gcgaggaccg gctctgcgtc cgcgggccgc tccattcggg
     2521 cgaagccgcc gcggtccgtg aagccgaggc cgccgtacac gggcgcaccc tggggcgtcg
     2581 cctcgaggcc cacgacctcg accgccttca gccgcgccaa cgcgtcccgc atgagacgcg
     2641 tcgcgatccc ctgccggcgc gcgtcggggt cgaccaggat catggctacc caggcgagcc
     2701 gccgaccgta cacgaccgcg ccgcccgagc cgaccaccgt ggcgtcgcgc acggccacgc
     2761 ggaagaagcc gttcgcgagc aagggctgcc agtcctccgt ccgctggttc cagcccgcga
     2821 gccggcaaag gcgcaggacc gccggcacgt cctccggcgt catgtcgcgg aagacgagcc
     2881 ccgccgg
//
LOCUS       DASPWO010000005         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_340342, whole genome shotgun sequence.
ACCESSION   DASPWO010000005 DASPWO010000000
VERSION     DASPWO010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340342"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VEO00_00095"
     CDS             complement(<1..305)
                     /locus_tag="VEO00_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HYU56434.1"
                     /translation="MVSIHEVRELALSLPRSSEALVRDRVKFRVGRIVYLAFSRDETL
                     MGFGFPKEERDALVRSEPDKFLMPHPRDLRYHWAVVRLAAIDRAEMREIVLDAWRMVV
                     "
     gene            339..1919
                     /locus_tag="VEO00_00100"
     CDS             339..1919
                     /locus_tag="VEO00_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYU56435.1"
                     /translation="MAGVGVSMSGGGHRASLFGLGVLLYLVDAGKNEQVTSISSVSGG
                     SLTNAFVGQALDLQATTPEEFDRAVAPFATRIARRGTFLGRGLTGRYPLVLLLTLFAT
                     AALWFLDGSRWFRFGLHLALVAVWALVVLSPMFGTLLGKLFGLAMAVTLVAALVAPWV
                     ANPPGWFGTPFGRFVLFVVALMLWVSLVFARRGAVCAHAYRRTLFTRDGRADRLASVQ
                     RGVDHVLCATELQSAEPVYFSSSFVYGYRFGLGDPSDLRLSVAAQASANLPFAFPARW
                     LRTAPFRFRYPKDAVRPAERGPGWRDCPPPEDRPEHKRFMVLSDGGVYDNMADQWPQG
                     FASRAGCWPDLGREHHEPDELIVANASAGLEWEPFKRSAIPAIGGLLSLLKVKDVLYD
                     QTTAQRRAGLVGRFDRAALAAREGHPQGMRGALVHIPQHALHVPRAFRGSRLWPERAG
                     RAERILPLLEPDAEAWIALAGRSTAVGTVLSALGDATAVDLLKHGYVLAMVNLHVILN
                     YPWLGLPPDERFTRLLAG"
     gene            complement(1929..>2197)
                     /locus_tag="VEO00_00105"
     CDS             complement(1929..>2197)
                     /locus_tag="VEO00_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56436.1"
                     /translation="ATPRTLYDLWSDAANADHPMTVLMSTKPGVHGTHAETAVPRRTL
                     VEALERRAGEVYSTSALRRRVAWWEAVADLSRGTSFTVTQGSPD"
BASE COUNT          277 a          801 c          780 g          339 t
ORIGIN      
        1 acgaccatcc gccaggcgtc gaggacgatc tcgcgcatct cggcgcggtc gatcgcggcg
       61 aggcggacga ccgcccagtg gtaccggagg tctcgaggat ggggcatcag gaacttgtcg
      121 ggctccgacc tcaccagcgc gtcgcgctct tctttcggga acccgaaccc catcagcgtc
      181 tcgtcacgcg agaacgccag gtagacgatc cgcccgaccc ggaacttgac ccgatcccga
      241 accagcgcct cggagctccg cggcagcgag agcgccagtt cgcgcacctc gtggatcgac
      301 accatcccgg cgacgacgat acggctacca tcggcggcat ggcaggggtc ggggtctcga
      361 tgtcgggggg cggccatcgg gccagcctgt tcgggctcgg cgtgctgctg tacctggtgg
      421 acgccggcaa gaacgagcag gtcacctcga tctcgtcggt ctcgggcggc tcgctgacga
      481 acgcgttcgt cgggcaggcg ctcgatctgc aggcgaccac gcccgaggag ttcgatcggg
      541 ccgtcgcgcc gttcgcgacg cggatcgcac gccgggggac gttcctgggc cgcgggctca
      601 ccgggcgata cccgctcgtg ctgctcctga cgctcttcgc caccgccgcc ctctggttcc
      661 tcgacggctc gcgctggttc cggttcggcc tgcacctggc gctcgtcgcc gtctgggcgc
      721 tggtggtgct gagcccgatg ttcggcacgc tgctcggaaa gctcttcggg ctggccatgg
      781 cggtcacgct ggtcgccgcc ctggtcgccc cgtgggtcgc gaacccgccc ggctggttcg
      841 gcacgccgtt cggccgcttc gtgctgttcg tggtcgcgct gatgctgtgg gtgtcactcg
      901 tcttcgcccg ccgcggggcc gtctgcgcgc acgcgtaccg gcggaccctg ttcacgcggg
      961 acggccgggc cgaccggctg gcgtcggtgc agcgcggcgt cgaccacgtg ctctgcgcca
     1021 ccgagctgca gagcgccgag cccgtgtact tctcgtcatc gttcgtctac ggctaccggt
     1081 tcggcctggg tgaccccagc gacctgcgac tgtcggtcgc ggcacaggcc tccgcgaacc
     1141 tgccgttcgc gtttccggcg cggtggctgc gcaccgcgcc gttccgattc cgctacccga
     1201 aggacgccgt gcgccctgcc gagcgcggcc cgggctggcg ggactgccca ccgccggagg
     1261 accggcccga acacaagcgg ttcatggtcc tctccgacgg cggcgtgtac gacaacatgg
     1321 ccgaccagtg gccgcagggg ttcgccagcc gcgccggctg ctggcccgac ctggggcggg
     1381 agcaccacga gccggacgag ctgatcgtgg cgaacgcgtc tgccggactg gagtgggagc
     1441 ccttcaagcg ctcggcgatc ccggcgatcg gcggtctgct ctcgttgctg aaggtcaagg
     1501 acgtgctcta cgaccagacc accgcccagc gccgggccgg cctggtcgga cggttcgacc
     1561 gagccgccct cgccgcccgc gagggccacc cgcaggggat gcgcggcgcg ctggtacaca
     1621 tcccacagca cgccctccac gtcccgcgcg ccttccgcgg aagccggctg tggccggaac
     1681 gggcggggcg ggccgagcgg atcctccccc tgctcgagcc cgacgcggag gcttggatcg
     1741 cgctcgccgg gcgcagcacg gcggtcggaa ccgtgctctc ggcgctggga gacgccaccg
     1801 ccgtcgacct gctgaagcac ggatacgtcc tggcgatggt gaacctgcac gtcatcctga
     1861 actacccgtg gctcggcctg ccgccggacg agcggttcac ccggctcctc gctggctgag
     1921 cccggcgctc agtccggaga tccctgcgtc acggtgaacg acgtcccccg ggacaggtcg
     1981 gccaccgcct cccaccaggc gaccctccga cgcagggcac tcgtcgagta gacctcaccg
     2041 gctcggcgtt cgagggcctc gaccagcgtc cggcgcggca cggccgtctc ggcgtgcgtg
     2101 ccgtgcacgc cgggcttcgt ggacatcagg accgtcatcg ggtggtcggc gttcgcggcg
     2161 tcggaccaca ggtcgtagag ggtgcggggg gtggcgt
//
LOCUS       DASPWO010000006         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1063, whole genome shotgun sequence.
ACCESSION   DASPWO010000006 DASPWO010000000
VERSION     DASPWO010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VEO00_00110"
     CDS             complement(<1..942)
                     /locus_tag="VEO00_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007354930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU56437.1"
                     /translation="MDATATALERGPLGRLARIGTLETDGPEEALRKETLVLSASTIT
                     ALAVVWVVTYWALGLHLSAAIPFAYQVISVVNLSLFARTKRYRFFRASELGLSLLLPF
                     LLQLSLGGFVPSSGVILWSFTAPLGALLFGGRREAVRWFAAFVGVIALAAALDPLLAN
                     RTAQIPRGLQIAFFALNVLGVTGTGYFLLHYFVHERDRAAAMAAAERERSERLLLNVL
                     PEPIAERLKAGEAVIADGIGEVGVLFADIAGFTPLSESMDPDDVVRLLDELFSEFDHL
                     ADRYRLEKIKTIGDAYMVASGLLEPDPRHAEHLAEMAL"
     gene            1027..2391
                     /locus_tag="VEO00_00115"
     CDS             1027..2391
                     /locus_tag="VEO00_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HYU56438.1"
                     /translation="MSGASGAILLLDGAVPSLDAYVEGGGGRGLAAALERAPEDVIEE
                     VRRAGLRGRGGAGFPTGMKWRTVREDPSATKYVVCNAAEGEPGTFKDRWLLRMNPYQL
                     LEGLAIAGHAVGAERAYIGIKAGFEREIERLRGAMEEMSAHDLLGPVPVELIGGPDEY
                     LFGEEKALLEVIEGNDPLPRVIPPWMEGLFREPGSPNPTAVNNVETLSNVPHILREGA
                     EWFRSFGTEDSPGTMVFTLCGDVRHPGMYELPMGLTLRDLIEGVGGGPPGGRALRAVF
                     PGASNTILSAEQLDTPLGFDPMRAVGSGLGSGGFVVYDDSACIVRATLAFSRFLYVES
                     CAQCPACKHGTGAITGLLERIDRGEGGEIDVETILARALTVTDAQRCALPTGETLIAQ
                     SAVHVFRSEFAEHFGSGCPRPRDIALPKIVDFNERAGAFRYDERYALKRPDWTYADDP
                     DPEH"
     gene            2418..2768
                     /locus_tag="VEO00_00120"
     CDS             2418..2768
                     /locus_tag="VEO00_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HYU56439.1"
                     /translation="MGEVRVGPVSSLPPGKVTGAGRWAVGNAAGDRFAVTRRCRHLYA
                     DLAGGTIDRNGCLVCPWHGSRYDVKTGRMVRGPQGIFAKIPGLDAFFIALTRVLPLGR
                     GKVVERSGTLYVRS"
     gene            2840..>3136
                     /locus_tag="VEO00_00125"
     CDS             2840..>3136
                     /locus_tag="VEO00_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004921168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU56440.1"
                     /translation="MSDNAILVENLQKRFKDVVALAGIDFEVPTGTVFGLLGPNGAGK
                     TTAVRILATILRPDGGRAEVMGHDVVHHAPAVRASIGLAGQNAAVDPNLTGRENL"
BASE COUNT          466 a         1099 c         1152 g          419 t
ORIGIN      
        1 cagagccatc tcggcgaggt gctcggcgtg gcgcgggtcc ggctccagca gccccgacgc
       61 gaccatgtag gcgtccccaa tcgtcttgat cttctccagc cggtagcggt ccgccaggtg
      121 gtcgaactcg ctgaacaact cgtcgagcag ccggacgacg tcgtccgggt ccatcgactc
      181 cgagagcggg gtgaacccgg cgatgtcggc gaacagcacg ccgacctcgc cgatcccgtc
      241 ggcgatcacg gcctcgccgg ccttcagccg ctcggcgatc ggctccggca ggacgttgag
      301 caggagacgc tcggatcgct cgcgctcggc cgcagccatg gcggcggccc ggtcgcgctc
      361 gtggacgaag tagtgcagca ggaagtaccc ggtgccggtc accccgagga cgttcagcgc
      421 gaagaaggcg atctggaggc cccgcgggat ctgggcggtc cggttggcga gcaaggggtc
      481 gagcgcggcg gccagcgcga tcaccccgac gaaggcggcg aaccaccgca cggcctcccg
      541 gcgcccgccg aacagcaggg cgcccagcgg cgccgtgaac gaccacagga tcaccccgct
      601 cgacgggacg aagccgccga gggaaagctg cagcaggaac ggcagcagca ggctgagccc
      661 cagctcgctc gcgcggaaga accggtaccg cttggtccgc gcgaacaggc tcaggttgac
      721 gacggagatc acctggtacg cgaacgggat ggcggccgac aggtggagcc ccagcgccca
      781 gtaggtgacg acccacacca cggccagcgc ggtgatcgtg ctggccgaga ggacgagcgt
      841 ctccttgcgc agcgcctcct cgggaccgtc ggtctcgagg gtcccgatcc tcgccaggcg
      901 cccgagcggt ccccgttcga gcgcggtggc ggtggcgtcc atcgcgcata gctaagcctg
      961 cgatcccgtc ccgccgcaat ctcccggcgt ttccggggac gggaagccgg gcatgggacc
     1021 ggcagcgtga gcggagcgtc cggagcgatc ctgctgctcg acggcgcggt gccgtccctc
     1081 gacgcctacg tcgagggcgg cgggggccgc ggcctggccg ccgcgctgga gcgggcgccc
     1141 gaggacgtca tcgaggaggt ccgccgcgcc gggctccgcg ggcggggcgg tgccggtttc
     1201 ccgaccggga tgaagtggcg gacggtccgc gaggatccga gcgccacgaa gtacgtggtg
     1261 tgcaacgccg ccgaggggga gccggggacc ttcaaggacc gctggctcct gcgcatgaac
     1321 ccgtaccagc tgctggaggg cctcgcgatc gcgggccacg cggtgggcgc ggagcgcgcc
     1381 tacatcggga tcaaggccgg cttcgagcgg gagatcgagc ggcttcgtgg cgccatggag
     1441 gagatgtcgg cccacgacct gctcggcccc gtgcccgtcg agctgatcgg cgggcccgac
     1501 gagtacctgt tcggcgagga gaaggcgttg ctcgaagtga tcgaggggaa cgaccccctg
     1561 ccgcgggtga tccccccgtg gatggagggg ctgttccggg agcccggctc gccgaaccca
     1621 acggccgtca acaacgtcga gaccctctcg aacgtgcccc acatcctccg ggagggcgcc
     1681 gagtggttcc gctcattcgg gaccgaggac tcgccgggga cgatggtctt caccttgtgc
     1741 ggcgacgtcc gtcacccggg gatgtacgag ctgccgatgg gcctcacgct tcgcgacctg
     1801 atcgagggcg tcggcggcgg cccgccgggc ggccgcgccc tgagggcggt cttccccggc
     1861 gcctcgaata cgatcctctc cgccgagcag ctcgacacgc cgctcgggtt cgacccgatg
     1921 cgggcggtcg ggtccgggct cggctccggg gggttcgtcg tctacgacga ctcggcgtgc
     1981 atcgtccgcg ccaccctggc cttctcccgc ttcctgtacg tcgaatcgtg cgcccagtgc
     2041 ccggcgtgca agcacgggac cggggcgatc accggcctcc tggagcggat cgaccgcggc
     2101 gagggcggag agatcgacgt ggagaccatc ctcgcccggg ccctgaccgt gaccgacgcc
     2161 cagcgatgcg cgctcccgac cggcgaaacc ctgatcgccc agagcgccgt gcatgtgttc
     2221 cggtccgagt tcgccgagca cttcgggagt ggatgcccgc gcccgcgcga catcgccctg
     2281 ccgaagatcg tcgacttcaa cgagcgtgcc ggggccttcc gctacgacga gcggtacgcc
     2341 ctgaagcggc ccgactggac gtacgcggac gaccccgatc cggaacacta gagtccggca
     2401 ggagattggg aggatccatg ggcgaggtgc gggtcgggcc ggtgtcgagc ctgccgccgg
     2461 ggaaggtgac cggggccggg cgctgggccg tcgggaacgc ggccggcgac cgcttcgcgg
     2521 tgacccggcg ctgccgccac ctctatgccg acctggccgg agggacgatc gaccggaacg
     2581 gctgcctggt ctgcccctgg cacggctcgc gctacgacgt gaagaccggt cggatggtgc
     2641 gcgggccgca ggggatcttc gcgaagatcc ccggcctcga cgcgttcttc atcgcgctga
     2701 cccgggtgct cccgctcggc cgcgggaagg tggtcgagcg cagcgggacc ctctacgtcc
     2761 ggtcgtagcg gccatttctc ccgcccgcgc taggctgccg gaaagtcccg tccggctcga
     2821 agcggaggac gcggcacccg tgagcgacaa cgccatcctg gtcgagaacc tccagaagcg
     2881 gttcaaggac gtcgtcgccc tggccggcat cgacttcgag gtgccgaccg ggaccgtctt
     2941 cggcctgctc ggcccgaacg gggccggcaa gaccacggcg gtgcggatcc tggcgacgat
     3001 cctgcgtccc gatggcggcc gggccgaggt catggggcac gacgtggtcc accatgcccc
     3061 cgccgtccgc gccagcatcg ggctcgccgg gcagaacgcg gcggtggacc cgaacctgac
     3121 cggccgtgag aacctc
//
LOCUS       DASPWO010000007         4340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1104231, whole genome shotgun sequence.
ACCESSION   DASPWO010000007 DASPWO010000000
VERSION     DASPWO010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104231"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VEO00_00130"
     CDS             <1..538
                     /locus_tag="VEO00_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HYU56441.1"
                     /translation="IMEMTIARDRWQEHLKSRGRHYHDSAAGLEALHGSRPEAGADAA
                     ALAAHALAELLERCRAARLTRNQHVLLRLGELIASVECAGSLARRADRALRGALGPKA
                     DHRFDPATLATLSRVFGREAAFRVAHEGLRLVLAADPAAAQADVAAALRLPEIDRLQQ
                     GLMEDMDRVADALYERAP"
     gene            535..>4340
                     /locus_tag="VEO00_00135"
     CDS             535..>4340
                     /locus_tag="VEO00_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYU56442.1"
                     /translation="MSRGAATEANESEVDMETRDERIAIVGVGAILPDAPTAAAFWEN
                     VRRGRYSITETPPDRWDPELYYDPDPRAPDKTYSKIGGWVRDFEWDPLGWRLPIPPLV
                     ADAMDDGQKWALACTREALFDYGYPERPLDEERTAVILGNAMAGEKHYLTAFRIMFPE
                     LAEELERAPSFMALTNDVQAAIVSELRTGIGKRFPEITEDTMPGELANILAGRIANLN
                     NFRGPSFTTDAACASGLAAISAAVHGLRAGDFDSVITGGVDRNMGIAAFVKFCKIGAL
                     SATGTRPYAEGADGFVMGEGAAVFLLKRLSDAERDGDRVHAVLVSLAGSSDGRGKGIT
                     APNPIGQRLAVERAWRAAGVAPSTATLVEGHGTSTAVGDLVEMESLQAAFGDQDLVPG
                     SIPLGSVKSNIGHLKSAAGAAGLLKTMFALRDGVLPPSLNFARPNHGIDWAGSPFRVN
                     TELTEWERPPGGVRRAGVSAFGFGGTNFHAVLEEHVPGARRGGGRAVVAVPETAALPK
                     VVPTTTSPGGSPKPPLRGAVVIGAETAEALRARLATVAGEAGAGRAPAPGVPEAADLR
                     APERLAIDYGDAADLATKAGAALKALAGDDPATWRALKGRGIFRGSGPAPKVAFLYTG
                     QGSQYANMLRSLRDAEPIVAETFAEADRVMAPLLDRPLSEHVFVDPDDPAAMGAAELD
                     LMRTEITQPAVLATDLALTRLLAAYGVRPDMVMGHSLGEYGALVAAGALTFEHALEAV
                     SARGREMASLAIEDNGKMAAIVAPLPEIERIVGAVDGYIEIANLNSNGQAVIGGASDA
                     VDRAVEAFTEAGFQAIPLPVSHAFHTAIVAPASRPLREALVRLELRPPALPIVANVSG
                     EFYPTEGDVTAQMLDILAAQVASPVQFVKGLLSLYEAGARAFVEVGPKKALSVFAEDV
                     LGERGDALTLFTNHPKVGDLASFNQALCGLYAAGLGAPAEAAPTTTEGPASIAREPAA
                     SPATPVTAPPDALVALFDEFRQRARDLVDRPAGDGGPERAPEVVVTGAALGLPGTEHV
                     FDDGNVGRILRGEQLIGPVPEGLRRAMLDKHVTRLVKRENGDPTFETIDDAADVIKVA
                     GRAGAFDLEAEFGVDADRIAALDRATELAIAAGFDALRDAGIPLVPKYRVTTKGTKLP
                     EGWTLPEEIGDDTGVIFASAFPGYDAFVDEVNRFHEDRGRRTELRDLERVRERIAERD
                     GHAGPALEELDRRIGELRMEIDAHPYRFDRRFLFKALPMGHSQFAEIVGARGPNTQIN
                     SACASTT"
BASE COUNT          603 a         1550 c         1640 g          547 t
ORIGIN      
        1 gatcatggag atgacgatcg cccgcgaccg ctggcaggag cacctcaaga gccgcgggcg
       61 ccactaccac gactccgccg cggggctgga ggcgctgcac gggtcccggc cggaagccgg
      121 cgccgacgcc gccgcgctcg ccgcgcacgc gctggccgag ctcctggagc ggtgccgggc
      181 ggcgcggctg acccgcaacc agcacgtcct gctgcgactc ggcgagctga tcgcctccgt
      241 ggagtgcgcc ggctcgctgg cccgacgggc cgaccgcgcc ctgcgcgggg cgctcgggcc
      301 gaaggcggac caccgcttcg atccggcgac gctcgcgacg ctctcgcgcg tcttcgggcg
      361 ggaggcggcg ttccgcgtgg cgcacgaggg cctgcgcctg gtgctggcgg ccgacccggc
      421 cgccgcccag gccgacgtcg ccgccgccct gcggctgccc gagatcgacc ggttgcaaca
      481 gggactgatg gaggacatgg accgagtggc cgatgcgctc tacgagcgtg ccccgtgagc
      541 aggggcgcgg ccaccgaagc gaacgagagc gaggttgaca tggagaccag ggacgagcgc
      601 atcgcgatcg tcggcgtggg cgcgatcttg ccggacgccc cgacggcggc cgccttctgg
      661 gagaacgtcc gccggggccg ctacagcatc accgagactc cgcccgaccg gtgggacccg
      721 gagctgtact acgacccgga cccgcgggcg cccgacaaga cctactccaa gatcggcggg
      781 tgggtgcggg acttcgagtg ggacccgctc ggctggcgcc tgccgatccc gcccctcgtc
      841 gccgacgcga tggacgacgg gcagaaatgg gccctggcct gcacccgcga ggcgctcttc
      901 gactacggct atccggagcg gccgctggat gaggagcgga ccgcggtgat cctcggcaac
      961 gcgatggcgg gcgagaagca ctacctcacg gcgttccgca tcatgttccc ggagctggcc
     1021 gaggagctcg agcgcgcccc gagcttcatg gcgctgacca acgacgtcca ggcggccatc
     1081 gtctcggagc tgcgcaccgg catcggcaag cgcttcccgg agatcaccga ggacaccatg
     1141 cccggcgagc tcgccaacat cctggccggg aggatcgcga acctcaacaa cttccgcggg
     1201 ccgagcttca ccaccgacgc cgcctgcgcc tcgggcctgg ccgcgatcag cgcggccgtc
     1261 cacgggttgc gggccggcga cttcgactcg gtgatcactg gcggcgtgga ccgcaacatg
     1321 ggcatcgcgg cgttcgtgaa gttctgcaag atcggcgccc tgtcggccac gggcacacgg
     1381 ccgtacgcgg agggggccga cgggttcgtc atgggcgagg gcgccgcggt cttcctgctg
     1441 aagcggctgt ccgacgccga gcgcgacggc gaccgcgtcc acgcggtcct ggtctctctg
     1501 gcggggtcga gcgacggcag gggcaagggc atcaccgccc cgaacccgat cgggcagcgc
     1561 ttggcggtcg agcgcgcctg gcgcgccgcc ggcgtggcgc cgtcgaccgc gacgctcgtc
     1621 gaagggcacg gcacctccac cgcggtcggc gacctggtgg agatggagag cctgcaggcc
     1681 gcgttcggcg atcaggacct cgttcccggg tcgatcccgc tcggctcggt gaagtcgaac
     1741 atcggccacc tgaagtcggc cgccggcgcg gcggggctgc tgaagactat gttcgcgctg
     1801 cgcgacggcg tcttgccgcc gtcgctcaac ttcgcgaggc cgaaccacgg gatcgattgg
     1861 gcgggctccc cgttccgcgt caacacggag ctcaccgagt gggagcggcc gccgggaggc
     1921 gtgcgccgcg ccggcgtcag cgcgttcggg ttcggcggga cgaacttcca cgccgtgctc
     1981 gaggagcacg tccccggcgc gaggaggggc ggcgggcgcg cggtcgttgc ggtgcccgag
     2041 acggcggccc tgccgaaggt tgtgcccacc acgaccagcc ctggtgggtc gccgaagccg
     2101 ccgctgcgcg gggcggttgt gatcggggcc gagaccgcgg aggcgctgcg tgcccggctg
     2161 gcgaccgtcg ccggggaggc cggcgcgggc cgcgccccag cgcccggggt gcctgaggcc
     2221 gcggacctcc gcgcgcccga gcgcctggcc atcgactacg gcgacgcggc cgacctggcc
     2281 accaaggcgg gcgccgccct gaaggcgctg gcgggggacg atccggcgac gtggagagcg
     2341 ctgaagggcc gggggatctt ccgcggaagc gggccggccc cgaaggtggc cttcctctac
     2401 accgggcagg ggtcgcagta cgcgaacatg ctgcggtcgc tgcgagacgc cgagccgatc
     2461 gtggccgaga ccttcgcgga ggccgaccgg gtgatggcgc cgctgctcga ccggcctctc
     2521 tccgagcacg tcttcgtcga cccggacgat cccgccgcga tgggcgccgc cgagctcgac
     2581 ctgatgcgga ccgagatcac ccagccggcg gtgctcgcca ccgacctggc cctgacccgc
     2641 ctgctcgccg cgtacggggt ccggcccgac atggtgatgg ggcacagcct gggcgagtac
     2701 ggcgcgctgg tcgcggccgg cgcgctgacg ttcgagcacg ccctcgaggc cgtcagcgcc
     2761 cgcgggcggg agatggccag cctggcgatc gaggacaacg ggaagatggc ggcgatcgtc
     2821 gcgccgctcc ccgagatcga gcggatcgtc ggcgcggtgg acggctacat cgagatcgcc
     2881 aacctcaact cgaacggcca ggccgtgatc ggtggcgcca gcgacgcggt cgatcgggcg
     2941 gtcgaggcct tcaccgaggc cggcttccag gccataccgc tcccggtgag ccacgctttc
     3001 cacaccgcga tcgtggcgcc ggccagccgg ccgctgcgcg aggcgctggt ccggctggag
     3061 ctgcgcccgc cggcgctgcc gatcgtggcg aacgtcagcg gcgagttcta cccgacggaa
     3121 ggcgacgtga cggcccagat gctcgacatc ctggccgcgc aggtggcctc accggtgcag
     3181 ttcgtgaagg ggctgctctc gctctacgag gccggcgcgc gcgccttcgt ggaagtcggc
     3241 ccgaagaagg cgctgtcggt gttcgccgag gacgtcctcg gcgagcgggg cgacgcgctg
     3301 acgctcttca ccaaccaccc caaggtgggt gacctggcct cgttcaacca ggcgctgtgc
     3361 gggctgtacg cggccggctt gggggctccg gccgaggccg cgcccaccac gaccgagggg
     3421 ccggcttcga tcgctcggga gccggccgcc tcgcccgcga cgcccgtgac ggctccgccg
     3481 gacgcgctgg tggccctgtt cgacgagttc cgccagcggg cccgcgacct cgtcgatcgg
     3541 ccggccggcg acggcgggcc ggagcgcgcc cccgaggtcg tggtcaccgg cgcggcgctc
     3601 ggtctgcccg gcaccgagca cgtcttcgac gacgggaacg tcggccggat cctgcgcggc
     3661 gagcagctga tcggcccggt cccggagggc ctgcgccggg cgatgctcga caaacacgtc
     3721 acgcggctcg tcaagcgcga gaacggcgac ccgaccttcg agacgatcga cgacgccgcc
     3781 gacgtgatca aggtcgccgg gcgcgctggc gcgttcgacc tcgaggccga gttcggcgtg
     3841 gacgcggacc ggatcgccgc gctcgaccgg gccacggagc tcgcgattgc ggccggcttc
     3901 gacgcgctgc gggacgcggg gatccccctg gtgccgaagt accgggtgac gaccaagggc
     3961 acgaagctgc cagagggctg gacgctcccg gaggagatcg gggacgacac cggcgtgatc
     4021 ttcgcgtcgg ccttccccgg ctacgacgcc ttcgtcgacg aggtgaaccg gttccacgag
     4081 gaccgcggac gccggaccga gcttcgggat ctcgagcggg ttcgcgagcg gatcgcggaa
     4141 cgggacggac acgccggacc ggcgctggag gagctcgacc ggcggatcgg ggagttgcgg
     4201 atggagatcg atgcccaccc gtaccggttc gaccgccgct tcctgttcaa ggcgctgccg
     4261 atggggcact cgcagttcgc cgagatcgtc ggggcgcggg gcccgaacac ccagatcaac
     4321 tcggcgtgcg ccagcaccac
//
LOCUS       DASPWO010000008         7962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_171181, whole genome shotgun sequence.
ACCESSION   DASPWO010000008 DASPWO010000000
VERSION     DASPWO010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7962
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171181"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VEO00_00140"
     CDS             complement(<1..394)
                     /locus_tag="VEO00_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYU56443.1"
                     /translation="MRPEPRTARISSGEMAFLDAGEGPAVVLLHGFPTSSHLWREFLP
                     LLASRFRTIAPDLIGYGASTKAPGTDLSIRAQAGYVRELLAGLGIEGGIAAVGHDIGG
                     GVAQLLAFEGVAACLVLIDAVTLDSWPIE"
     gene            complement(402..1244)
                     /gene="folP"
                     /locus_tag="VEO00_00145"
     CDS             complement(402..1244)
                     /gene="folP"
                     /locus_tag="VEO00_00145"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HYU56444.1"
                     /translation="MDDDFVWRAGPYRLDCGARTHVMGIINATPDSFSDGGRFLDRAA
                     AVKRGIEMVTEGADLLDVGGESTRPGSDPVSVEGERERVLPVIRGLAAEVDVPISIDT
                     RKHEVAGAALEAGATIVNDVTAGSDPRMFGLVRDAGAGMVLMHMRGEPKTMQQDTRYD
                     DVVAEVRDYLAARVTAAVEAGVGWDRLCVDPGLGFGKDVEGNLRLVKEVDAFLDLRRP
                     VLVGPSRKSFVGNVTGTEVAERLEGTAAAVAWMAGRGAHVVRVHDVKEMVRVVRMVDA
                     IRRA"
     gene            1330..1848
                     /locus_tag="VEO00_00150"
     CDS             1330..1848
                     /locus_tag="VEO00_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HYU56445.1"
                     /translation="MIGAVEVAERGRTEGGRLAELYRRHAPDAVRLAYLLTGDRVLAE
                     DLVQDAFVKLAGRFRDLRDPDAFAAYLRRTVVNLTNSHWRRTRLERAHAEREGRRLRD
                     APVAAAGTEERDELWTALRRLPRRQRAAIVLRYYEDLSEARIAEVLGVRPGTVKSLVS
                     RGMDALRAEVTR"
     gene            1854..2876
                     /locus_tag="VEO00_00155"
     CDS             1854..2876
                     /locus_tag="VEO00_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56446.1"
                     /translation="MHENDVRELLQRKADEVRLDAVIPQRMLTRARRRRMTTVAASAL
                     SLVVLVAGGLAGARILAGGDRPGPADTSPAPSPSPSARPTPSPSVVPTPVKDFVNAFL
                     AARIAGSGAEDAFLTEDSAAMYATHQGGLYLYDGDDPDGQPEVNYHSASIDSVSARGD
                     GSYDVVVVIEAGEFEGVTGAFVEELWIGQGLRLDGSRGWVVRQAQRVRAVGGETVKHC
                     FEGSATPVRDCARAFMQTRLDGAGAEFYLTEDARALYDDPSNDLHLYGQPYGDSGGLD
                     FQSWLVQAIAKRADTFLVDVELEACPADGGSCFTIFETLTLGPGRNQNGELQDFIVRS
                     AEISPA"
     gene            2975..3181
                     /locus_tag="VEO00_00160"
     CDS             2975..3181
                     /locus_tag="VEO00_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56447.1"
                     /translation="MATKLEVAGLFQRLQEALGGRLSAEPAPADDEAYTAAEDEIRGL
                     THPADISVEDEPARPLDALRVQRR"
     gene            3232..3792
                     /locus_tag="VEO00_00165"
     CDS             3232..3792
                     /locus_tag="VEO00_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU56448.1"
                     /translation="MKQDGPSLRLLLVETAAPSRAAETGALDPFESFFDATYERLLRA
                     LYLVTGDRHEAEDLAQEAFVRAYERWDRVSRTGNPAGYLYRTALNAYRSRLRRIAVAA
                     RRAMRRIERDPIDESDDRDEIRRALAALPEGQREALVLVEWLGLTDEEAGAALGVKAV
                     TVRVRMTRARQALRERSHEPEEGTER"
     gene            3789..4817
                     /locus_tag="VEO00_00170"
     CDS             3789..4817
                     /locus_tag="VEO00_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56449.1"
                     /translation="MNDLRELLDREAGRVRADVGALERMVRRADHRRRNRRITSGVVA
                     LAVAAAGFVGVVSALGGGGTRPAAGADWRAVWPQQSRADAETAQERANRGDGNLAWQQ
                     DAEAFLWQFGNDGLQWVNTTIPAVGPAEAAGNGPLTVTIDGYPYERCDDTAAWVCPEE
                     ALKHARITIERLLQPDPTGIWFVTDVVVDGEHPAVPDSPLPPVGVPQPSDTAQPSDTA
                     GSGATVQPTVVGPPDEVADFVNAFMDSRVSGTDFARDRLSDEAATAYHSGEGGLALYG
                     YTREANAVWTLGSWSGVPDTDRWRFVVQIRGAGDAFPDSGIIQETLLVGSPGDGAPPV
                     VLTAERNE"
     gene            complement(4825..5043)
                     /locus_tag="VEO00_00175"
     CDS             complement(4825..5043)
                     /locus_tag="VEO00_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HYU56450.1"
                     /translation="MSVRVRIDRTRCIGAETCIQLAPTAFKWRDEELGKADLLDPASV
                     EDDLLREAAAACPTLAIILTEEDDPAQV"
     gene            complement(5040..>7962)
                     /locus_tag="VEO00_00180"
     CDS             complement(5040..>7962)
                     /locus_tag="VEO00_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HYU56451.1"
                     /translation="LARLMLSSPAVALAADAASRRGLKAAEGALQGPAEPEMASLSAE
                     DRMVRAFDRAAVALRAVAGRKPLFLALDDLQWADEDSLRLIRYLVRTEPNVPLLLVLA
                     FRREAARGASALLGLSVDAERLGVGRRVMLERLTAPEATELLQHLLGGPASSACAQAL
                     YGQSEGVPFILEELVRTFRENGTVRSIEGTWQIAGSVRVVPSSVQSLIERKAAALPEA
                     TREVLGDAAAIGRRFSLQDLAAVTRALRGDGGPGPADLATMLTPAVHSGLLAEVPEGG
                     RADYTFTHDSARAVLAEAQGRPRRRLAHRAVLDLIADGGEPPRHRLAEAAHHAILGGE
                     RERGVRYALQAARDALTAGAGDEALRIVAGAMTATDAADDQLELLRLQDDALEMLGRP
                     VDRGRVLERFARVLEGRRDEGLEFELMRRRASAARGSGDVELAADLARLTREQAAAAG
                     NRRAELEACLELGQALMRAPLGEAYNPPPTETDLDAAEEAFQRALALADEAGEERLTA
                     ACLRELGAIQNGRAKGMAVEQITPSVLETGRFHLPADDDPRWGPIDERHTTGKAYLER
                     ALEIFERLDDRAGVMSTVIALSYAHYHSGVERGVAGVIEQIRRLRSRLKSMTTESERQ
                     RDEAEMLFSVHAYARFYHQADLALLRGIEAHRAARSFGIRWLEFASAGGVALTHLELG
                     EPDEAEEWLDRARAAAMVQLGPLRARQLDLWRGLARAASGDVDGAVERLERARTLATE
                     QRNRPGQTEAMTALALQGALLAAERDDAELARRAEPWAEAALALAGAQVGHHPWRAQA
                     RAAIAQLALLRGDPSAAADQVLLAVAEMDARLLEPVLFEVLLVAARVARAADRTDRDQ
                     VLGRIRWDVADVAERIVDDDVRARWLAAPVQAAIVDIAGRPEPGAPRPASGRAARLLT
                     DHERRILAGVARGMTNREISAELDLDVRSVGKDLAGVFTKLGVRSRGAATTVALREGL
                     A"
BASE COUNT         1054 a         2991 c         2906 g         1011 t
ORIGIN      
        1 cctcgatcgg ccacgagtcg agcgtcaccg cgtcgatcag gaccaggcac gcggcgacac
       61 cctcgaaggc cagcagctgc gccaccccgc ccccgatgtc gtggccgacc gccgcgatgc
      121 cgccctcgat ccccagtccg gcgagcagct cccggacgta cccggcctgc gcccggatcg
      181 acaggtcggt gccgggcgcc tttgtcgagg cgccgtagcc gatcaggtcg ggagcgatgg
      241 tccggaaccg agatgcgagc agggggagga actcgcgcca gaggtgggac gaggtcggga
      301 acccgtgcag caggacgacc gccgggccct cgcccgcatc gaggaacgcc atctccccgc
      361 tcgagatgcg cgcggtcctc ggctccgggc gcacggccgc gtcaggcccg cctgatggcg
      421 tcgaccatcc gaaccacgcg caccatctcc ttgacgtcgt ggacgcggac gacatgcgcg
      481 ccgcgcccag ccatccaggc caccgccgcg gccgtgccct ccaggcgctc ggccacctcg
      541 gtgccggtga cgttcccgac gaacgacttg cgggacgggc cgaccagcac gggccggcgc
      601 aggtcgagga atgcgtcgac ctctttcacc agccgcaggt tgccctcgac gtccttcccg
      661 aacccgagcc cggggtcgac gcacagccgg tcccacccca caccggcctc gaccgcggcc
      721 gtcacgcggg cagcgaggta gtcccgcacc tcggcgacca cgtcgtcgta gcgcgtgtcc
      781 tgctgcatgg tcttcggctc gccacgcatg tgcatcagca ccatcccggc gcccgcgtcg
      841 cgcaccaggc cgaacatgcg cgggtcggac ccggcggtga catcgttgac gatcgtcgcg
      901 ccggcctcga gggccgcccc ggccacctcg tgcttgcgcg tgtcgatcga gatcggcacg
      961 tcgacctcgg cggccaggcc ccggatcacc ggcaggacgc gttcgcgctc cccctcgacg
     1021 gagacgggat ccgagcccgg gcgggtcgac tcgccgccga cgtcgaggag gtccgcgccc
     1081 tccgtgacca tctcgatccc gcgcttcacc gccgccgcgc ggtcgaggaa ccggccgccg
     1141 tcggagaacg agtcaggcgt cgcgttgatg atccccatca cgtgggttcg cgctccacag
     1201 tcgaggcggt aggggcccgc ccgccagacg aaatcgtcgt ccacgaccgg atcgtagcga
     1261 aggccggggg accgccccgc gggcccggcc gcggaaccgg atctcgcccc cgtgcgtcca
     1321 ccattcgtca tgatcggcgc ggtcgaggtg gcggagcgag gacggaccga gggcgggcgg
     1381 ctggccgagc tgtaccggcg gcacgcgccg gacgcggtgc ggctcgccta cctgctgacc
     1441 ggcgaccgtg tcctcgccga ggacctggtg caggacgcgt tcgtcaagct ggccgggcga
     1501 ttcagggacc tgcgtgaccc cgacgccttc gcggcgtacc tgcgccggac cgtggtgaac
     1561 ctgacgaact cgcactggcg ccgcacgcgc ctggagcggg cgcacgccga gcgcgagggc
     1621 cggcgcctcc gcgacgcgcc ggtcgccgcg gccgggaccg aggagcgcga cgagctgtgg
     1681 acggcgctcc gccgcctgcc ccggcggcag cgggccgcga tcgtgctgcg ctactacgag
     1741 gacctgtccg aggcgcggat cgccgaggtg ctcggcgtgc ggcccggcac cgtgaaatcc
     1801 ctcgtctcca ggggcatgga tgccctgcgg gccgaggtca cgaggtgagc gagatgcacg
     1861 agaacgatgt gcgcgagctg ctgcagcgca aggccgatga ggttcgcctc gacgcggtga
     1921 tccctcagcg catgctgacc cgggcgcggc ggcggcggat gacgaccgtg gcggcgtcgg
     1981 ccctctcgct ggtggtcctg gtcgccggcg gactggccgg cgcccgcatc ctggccggcg
     2041 gcgaccgccc gggcccagcc gacacctctc cggccccgag cccgtccccg agcgcgcggc
     2101 ccaccccttc ccccagcgtg gtccccacgc cggtcaagga cttcgtgaac gccttcctgg
     2161 ccgcgcggat cgccggctcc ggcgccgagg acgccttcct cacggaggac tccgcggcga
     2221 tgtacgccac ccaccagggc ggcctctatc tctacgacgg tgacgacccc gacggtcagc
     2281 ccgaggtcaa ctaccactcc gcgtccatcg actccgtgtc ggcccgtggc gacggaagct
     2341 acgacgtcgt ggtcgtcatc gaggcgggcg agttcgaggg ggtcaccggc gcgttcgtgg
     2401 aggagctctg gatcgggcag ggcctgcgac tcgacgggtc acgcggatgg gtggtccggc
     2461 aggcgcagcg cgtccgcgcc gtcggtggcg agaccgtcaa gcactgcttc gaggggagcg
     2521 cgacgccggt gcgcgactgc gcgcgggcgt tcatgcagac ccggctggac ggcgccggcg
     2581 cggagttcta cctgaccgaa gacgcccgcg cgctgtacga cgacccgtcg aacgacctgc
     2641 acctgtacgg ccagccctat ggggactccg gcgggctcga cttccagtcc tggctggtcc
     2701 aggcgatcgc caagcgagcg gacaccttcc tggtggatgt cgagctcgag gcgtgccccg
     2761 cggacggcgg gtcctgcttc accatcttcg agacgctgac gctgggcccg ggccggaacc
     2821 agaacggcga gctgcaggac ttcatcgtcc gctccgccga gatctcgccg gcctgagcct
     2881 cgtaaccgat cgaccgggcg gacggtcgaa gcgttcgact ccttccgtgc cgggcgcgtt
     2941 cgtcctaggg tggggggaac cggggggtgg gccgatggca acgaagctcg aggtcgcggg
     3001 attgttccag cgcctgcagg aggcgctggg gggccgcctc tctgccgagc ccgcacccgc
     3061 cgacgacgag gcctacacgg ccgccgagga cgagatcagg ggcctgacgc acccggccga
     3121 catctcagtc gaggacgagc ccgcgaggcc gctcgacgca cttcgcgtgc agcgccggta
     3181 ggcgcccccc ttgctcgggc gcgccggtac gctgcgcccg atgatggcgc cgtgaaacag
     3241 gatggtccct ccctgcgtct ccttctcgtg gagacggcag cgccgtcacg agcggcggag
     3301 accggcgccc tggacccgtt cgagtccttc ttcgacgcca cgtacgagcg cctgttgcgg
     3361 gccctctacc tcgtcaccgg ggaccggcac gaggcggagg acctggcgca ggaggccttc
     3421 gtgcgggcct acgagcgctg ggaccgggtg tcgaggaccg gcaacccggc cggctacctg
     3481 taccgcaccg cgctgaacgc gtaccgcagc cggctgcggc ggatcgcggt ggcggcgcgg
     3541 cgcgccatgc gccgcatcga acgcgacccg atcgacgagt cggacgaccg cgacgagatc
     3601 cgccgcgcgc tcgccgcgct ccccgagggg cagcgcgagg cgctcgtcct agtcgagtgg
     3661 ctcggcctga ccgacgagga ggccggcgcg gcgctggggg tcaaggcggt gacggtgcgc
     3721 gtgcggatga cgcgagcgcg gcaggcgctg cgcgagcgaa gccatgaacc cgaggagggc
     3781 acggagcgat gaacgacctg cgtgagctgc ttgaccggga ggcaggccgc gtgcgcgccg
     3841 acgtgggcgc cctggagcgc atggtgcgcc gcgccgatca ccgccggcgc aatcgccgca
     3901 tcacctccgg cgtcgtcgcc ctggcggtcg ccgccgcggg cttcgtggga gtcgtctccg
     3961 ccctcggcgg cggcgggacc cgccccgcgg ccggcgcgga ctggcgggct gtctggccgc
     4021 agcagtcccg cgcggacgcc gagacggcgc aggagcgcgc gaaccggggc gatggcaacc
     4081 tggcctggca gcaggacgcc gaggccttcc tgtggcagtt cggcaacgac gggctgcagt
     4141 gggtgaacac gacgatcccg gcggtggggc ccgccgaggc ggccggcaac gggccgctca
     4201 cggtcaccat cgacgggtat ccgtacgaac gctgcgacga caccgctgcc tgggtctgcc
     4261 ctgaggaggc gctgaagcac gcccggatca cgatcgagcg gctccttcag cctgacccga
     4321 cggggatctg gttcgtgacc gacgtcgtgg tcgacggcga gcaccccgcg gtgccggatt
     4381 ccccccttcc ccctgtgggc gtgccgcagc cgagcgacac ggcgcagccg agcgacacgg
     4441 cggggtcggg agctacggtg caaccgaccg tcgtggggcc cccggacgag gtcgcggatt
     4501 tcgtgaacgc gttcatggac tcccgggtct ccggcacgga tttcgcccgg gaccggctga
     4561 gcgacgaagc cgcgaccgcc taccacagcg gcgagggcgg gctcgcgctg tacggctaca
     4621 cgcgggaagc gaacgcggtc tggacgctcg gcagctggag cggggtcccg gacaccgacc
     4681 gctggcggtt cgtggtgcag atccgcggcg ccggcgacgc gttccccgac tcggggatca
     4741 tccaggagac gctcctggtc ggatcgcccg gcgatggtgc ccccccggtc gtcctcaccg
     4801 ccgagcgcaa cgagtagccg ccggtcagac ctgggccggg tcgtcctcct cggtgaggat
     4861 gatcgccagg gtcgggcagg ccgcagcggc ttcccgcagg aggtcgtcct cgacgctcgc
     4921 cgggtcgagc aggtccgcct tgcccagctc ctcgtcgcgc cacttgaacg ccgtcggcgc
     4981 gagctggatg caggtctcgg cgccgatgca acgggttcgg tcgatccgca cccggacgct
     5041 catgccaagc cctcccggag cgcgaccgtg gtggcggccc cccgcgagcg caccccgagc
     5101 ttggtgaaca cgcccgcgag gtccttcccc accgagcgga cgtcgaggtc gagctccgcg
     5161 gagatctcgc ggttggtcat cccgcgggcg acccccgcca ggatccggcg ctcgtggtcg
     5221 gtcagcaggc gcgcggctcg tccggaggcg ggccgggggg ctcccggctc cgggcgaccg
     5281 gcgatgtcga cgatcgcggc ctggacgggc gcggcgagcc agcgcgcccg aacgtcgtcg
     5341 tcgacgatgc gttccgccac gtccgccacg tcccagcgga tccggccgag gacctggtcc
     5401 cgatcggtcc ggtcggcggc ccgggcgacg cgcgccgcca ccagcagcac ctcgaagagg
     5461 acgggttcca ggagccgggc gtccatctcg gccaccgcga gcaggacctg atccgcggcc
     5521 gccgaggggt cgccgcgcag cagcgcgagt tgggcgatcg cggcgcgcgc ctgggcccgc
     5581 cacgggtgat ggccgacctg cgcaccggcc agagcgagcg ctgcctcggc ccatggttcc
     5641 gctcgccggg ccagctcggc gtcgtcccgc tccgcggcga gcagcgcccc ctgcagcgcc
     5701 agggcggtca tcgcctcggt ctgcccgggg cggttgcgct gctcggtggc cagggtccgg
     5761 gcacgctcga ggcgttcgac ggccccgtcg acgtcccccg aggccgctcg cgccagcccg
     5821 cgccagaggt cgagctgccg ggcgcgcagc ggcccgagct ggaccatcgc ggccgcccgc
     5881 gcccgatcga gccattcctc ggcctcgtcc ggttcgccga gctcgagatg ggtcagcgcg
     5941 acgccgcccg ccgaagcgaa ctccagccag cggatgccga aggaccgcgc cgcgcggtgg
     6001 gcctcgatgc cgcgcagcag cgcgaggtcc gcctggtggt agaaccgcgc gtaggcgtgc
     6061 accgagaaca gcatctcggc ctcgtcgcgc tggcgctcgc tctccgtggt catggacttc
     6121 agccgcgacc gcagcctgcg gatctgctcg atcaccccgg cgaccccgcg ctcgaccccc
     6181 gagtggtagt gcgcgtacga aagcgcgatc accgtgctca tcacgcccgc tcgatcgtcc
     6241 aggcgctcga agatctcgag ggcacgttcg agataggcct tgccggtcgt gtgccgttcg
     6301 tcgatcggtc cccagcgcgg gtcgtcgtca gcggggagat ggaaccgccc ggtctccagc
     6361 accgaggggg ttatctgttc caccgccatg cccttggccc ggccgttctg gatcgcgccg
     6421 agctcgcgca ggcacgccgc ggtcaggcgc tcctccccag cctcgtcggc gagggccagc
     6481 gcgcgctgga aggcctcctc cgctgcgtcg agatcggtct cggtcggtgg cgggttgtag
     6541 gcctcgccga gcggagcgcg catgagggcc tggccgagct ccaggcatgc ttcgagctcc
     6601 gcacggcggt tgccggcggc ggccgcctgc tctcgggtca accgggcgag gtcggcggcg
     6661 agctccacgt cgcccgaccc acgcgccgcc gacgcccggc ggcgcatcag ctcgaactcg
     6721 aggccctcgt cacggcggcc ctccagcacc cgggcgaacc gctccaggac ccgaccccgg
     6781 tccacgggcc ggccgagcat ctcgagcgcg tcgtcctgca ggcgcagcag ctcgagctgg
     6841 tcgtccgcgg cgtccgttgc cgtcatcgcg ccggcgacga ttcgcaacgc ctcgtcgccc
     6901 gcccccgccg tcagggcgtc ccgggcggcc tggagcgcgt agcgcacgcc gcgctcgcgc
     6961 tcgccgccga ggatcgcgtg atgggcggcc tccgccagcc ggtgacgcgg cggctcgccg
     7021 ccgtccgcga tcaggtcgag gacggcgcgg tgggccagcc tccgccgggg ccggccctgc
     7081 gcctccgcca ggactgcccg cgccgaatcg tgggtgaatg tgtagtcggc ccgcccgccc
     7141 tccggcacct cggcgagcag gccgctgtgg acggccggcg tgagcatcgt cgccaggtcg
     7201 gcgggtccgg gacccccgtc gccgcggagc gcgcgcgtga cggcggcgag gtcctgcagc
     7261 gagaatcggc ggccgatcgc cgcggcgtcc cccagcacct cacgggtggc ctccggcagc
     7321 gccgccgcct tccgctcgat cagcgactgc accgacgacg ggaccacgcg cacagatccc
     7381 gcgatctgcc acgtcccctc gatcgagcgg acggtgccgt tctcgcggaa cgtccggacg
     7441 agctcctcga ggatgaaggg aacaccctcg ctctggccgt acagcgcctg ggcgcacgcc
     7501 gagctggccg gcccgccgag caggtgctgc agcagctcgg tggcctccgg cgcggtcaga
     7561 cgctccagca tgacgcgccg ccccacgccg agccgctccg cgtcgaccga gagcccgagc
     7621 agcgcgctgg caccgcgcgc ggcctcccgc cggaaggcga gcaccaggag cagcgggacg
     7681 ttcggctcgg tgcggaccag gtaccggatc aggcgcaggc tgtcctcgtc cgcccactgc
     7741 aggtcgtcga gcgcgaggaa gagcggcttg cgccccgcga cggcgcgcag cgccaccgcc
     7801 gcccggtcga acgcgcggac catcctgtcc tcggcggaga ggctcgccat ctcgggctcc
     7861 gccgggccct gcagcgcgcc ctcggccgcc ttcagcccgc gccgcgacgc cgcgtccgcg
     7921 gccagggcga cggccggtga ggacagcatc aggcgcgcca gg
//
LOCUS       DASPWO010000009         2798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_86095, whole genome shotgun sequence.
ACCESSION   DASPWO010000009 DASPWO010000000
VERSION     DASPWO010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86095"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..858
                     /locus_tag="VEO00_00185"
     CDS             256..858
                     /locus_tag="VEO00_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU56452.1"
                     /translation="MEAERRALSQDREDRPTFQDLAREHLPRLYGLARRLAGDDAEDL
                     VQEALMRGFRAFASLERAESAGAWLSAILLNSHRDRLRKEARSVREISMEEVDDFSLY
                     RHIAEEDPYPYSDTLHADFLGLFDADDVHRVLDTLPPLYRAPLVLRYIEEYATKQIAR
                     VLDAPLGTILARLHRGRKLFERALWDYAEVTGLLEKEEVR"
     gene            855..1088
                     /locus_tag="VEO00_00190"
     CDS             855..1088
                     /locus_tag="VEO00_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYU56453.1"
                     /translation="MIACADAVKQLWDYLDRTLPEIDRVRVQEHLEVCRTCCGELEFA
                     QELQAFLAAQRDDEIPPDARARLEALLADLKES"
     gene            1088..1666
                     /locus_tag="VEO00_00195"
     CDS             1088..1666
                     /locus_tag="VEO00_00195"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYU56454.1"
                     /translation="MAHDLLHQEEIRDVVRRAYGALPSGGGRAVAERLYSDEELAAVP
                     QGAIDWALGVGNPVRDAGLRPGETVLDVGSGGGIDTILAARDVGSSGRVIGLDLLEEM
                     CERARAHARQAGVRGWTEFRRGEMEAIPLPDETVDVVISNGVINLSARKSRALAEIHR
                     VLRPGGRLCVTDLVVDDDLPQEVLTSDSAWAG"
     gene            1928..2137
                     /locus_tag="VEO00_00200"
     CDS             1928..2137
                     /locus_tag="VEO00_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HYU56455.1"
                     /translation="MKRLQISIDEDLDEALAIEAARRRTSKAALIRQYVRQRLGGRAI
                     RDPMAALIGDIDDEAGDIDEAGYGT"
     gene            2134..2535
                     /locus_tag="VEO00_00205"
     CDS             2134..2535
                     /locus_tag="VEO00_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYU56456.1"
                     /translation="MRFVDTSFWIGLRFRRDRRHPEARAIWESGPGPLLTTSLVVGET
                     WTFLARRAGHRRAVQFLDAASRLPNLTVRHVDEDAEQEAWRWLRRHDERTYSFVDATS
                     FVLMKRLRLREALAFDDDFASAGFVEVRPPT"
     gene            complement(2540..>2798)
                     /locus_tag="VEO00_00210"
     CDS             complement(2540..>2798)
                     /locus_tag="VEO00_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005456474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HYU56457.1"
                     /translation="QAMAEVGVDLSREFPKPLTDGVVRAADVVVTMGCGDACPVYPGR
                     RYLDWDLPDPAGKDVAEVRPIRDEIDRRVRGLFAELIPAAS"
BASE COUNT          429 a          973 c          989 g          407 t
ORIGIN      
        1 ccggatgaag aagatcaccg cgtacccgat gaacccgacc gcagccagac ccatgagcca
       61 cgatccccat cgcagcgccg agtcggcggc gtgctcactc ggtcgtgcca tcgctgcagt
      121 tgccatgccg gcctcccttc ggatgtcccc acccgtagag acgagggggc aggacccagt
      181 attgcgcgag ccaccggatg caataagttc gtcccctcgc tcgtcagtac ggacgtgagc
      241 cgagggagcc ggaccatgga ggccgaacga agggccctgt cacaggaccg ggaggaccgc
      301 ccgacgttcc aggaccttgc ccgggagcat ctcccccgcc tctacggcct ggcacgacgg
      361 ctggccggcg acgacgccga ggacctggtg caggaggccc tgatgcgcgg gtttcgcgcg
      421 ttcgcgtcac tggaacgcgc ggagtcggcg ggagcctggc tttccgccat cctgctgaac
      481 agccaccgcg accggctccg gaaggaggca cggtcggtgc gggagatcag catggaggag
      541 gtggacgact tctcgctgta ccgccacatc gccgaggagg acccgtaccc ctactcggac
      601 acgctgcacg cggacttcct cggcctgttc gacgccgacg acgtccaccg ggtcctcgac
      661 accctgcccc cgctctatcg cgcgccactc gtgctgcgat acatcgagga gtacgccacc
      721 aaacagatcg cccgggtcct ggacgcgccc ctggggacga tcctcgcccg cctgcatcgc
      781 gggcggaagc tcttcgagag ggccctgtgg gactacgcgg aggtcaccgg cctgctcgag
      841 aaggaggagg ttcggtgatc gcctgcgccg acgccgtgaa gcaactgtgg gactacctgg
      901 atcgcacgct gcccgagatc gatcgggtgc gcgtccagga gcatctggag gtctgccgga
      961 cgtgctgcgg cgagctcgag ttcgcccagg agctgcaggc cttcctggcc gcccagcgcg
     1021 acgacgagat cccaccggac gcgcgcgccc gcctggaggc gctgctggcc gacctgaagg
     1081 agtcgtgatg gcgcacgacc tgctgcacca ggaagagatc agggacgtcg tccgcagggc
     1141 ctacggcgcg ctcccctccg gcggcggccg tgcggtcgcc gagcggctgt attcggacga
     1201 ggagctcgcc gcggttccgc agggcgcgat cgactgggcc ctcggcgtcg gcaaccccgt
     1261 gcgcgatgcc gggctccggc cgggcgagac cgtcctggac gtgggttccg ggggagggat
     1321 cgacacgatc ctggccgccc gggacgtggg ctcatccggc cgggtcatcg gcctggatct
     1381 gctcgaggag atgtgcgagc gagcccgcgc tcacgcgcgc caggcggggg tccgcggctg
     1441 gaccgagttc cgccgcggcg agatggaagc gatcccgctc cccgacgaga ccgtggacgt
     1501 cgtcatctcg aacggggtga tcaacctgtc cgcgcggaag agccgcgccc tggcggagat
     1561 ccaccgggtc ctgaggcccg gcgggcggct ctgcgtcacc gacctggtgg tggacgacga
     1621 cctgccacag gaggtcctga ccagcgactc cgcgtgggcc gggtgactgt cgggcgccct
     1681 ggcggagcgt gtcttcgccg cgaagctgga gaaggcgggg ttcacccgcg ccgagttctc
     1741 ctcgcgcggg gcgttcagca tcgacgacgc cgccgccttc ccgctgttca cacccgagtt
     1801 gatcgggctg atgcgcaggc tggtcccgcc ggagcgccag acgcgcgtcg ccacctcggt
     1861 cctcgtgcgc gcccgccggc catgaacgat ggtgtaacta tctcggctgt gatgtaacct
     1921 cacccccgtg aaacgactgc agatcagcat cgacgaggac ctggacgagg cgctggcgat
     1981 cgaggccgct cgccgccgga cctccaaggc cgccctgatc cgtcagtacg tccggcagcg
     2041 cctaggggga cgagcgatcc gggacccgat ggcggcgctg atcggggaca tcgacgacga
     2101 ggcaggtgac atcgacgagg cgggctacgg gacgtgaggt tcgtcgacac gtcgttctgg
     2161 atcggcctcc gtttccgccg ggaccgtcgc caccccgagg ctcgggcgat ctgggagtcc
     2221 ggccccgggc ccttgttgac gaccagcctg gtcgtcggtg agacctggac gttcctcgcc
     2281 cggcgggccg ggcaccggcg cgccgtgcag ttcctggacg ccgcatcacg tctccccaac
     2341 ctgaccgtgc gccacgtcga cgaggacgcc gagcaggaag catggcgatg gctgcgcaga
     2401 cacgacgagc gcacctactc gttcgtggac gcgacgagct tcgtcctgat gaaacggcta
     2461 cgcctccgag aggccctggc cttcgatgac gactttgcgt ccgctggctt cgtggaggtt
     2521 cgtccgccga cctgaggcct caggacgcgg ccggaatcag ttcggcgaag aggccgcgga
     2581 cgcggcggtc gatctcgtcg cggatcgggc ggacctctgc gacgtccttg ccggcggggt
     2641 cgggcaggtc ccagtcgagg tagcgcctcc cggggtacac ggggcaggcg tcaccgcagc
     2701 ccatggtgac caccacgtcg gcggcccgga ccaccccgtc ggtcagcggc ttcgggaact
     2761 cgcgcgagag gtccacgccg acctcggcca tggcctga
//
LOCUS       DASPWO010000010         6269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_425574, whole genome shotgun sequence.
ACCESSION   DASPWO010000010 DASPWO010000000
VERSION     DASPWO010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425574"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VEO00_00215"
     CDS             complement(<1..475)
                     /locus_tag="VEO00_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HYU56458.1"
                     /translation="MATLFDRIDDVVIEEEIQSSFIDYAMSVIVSRALPDARDGLKPV
                     HRRILFSMMEGGLSPDRPRRKSAAAVGDVIKKYHPHGDQPVYDAMVRMAQDFSLRYPL
                     IDPQGNFGNVDGDPPAAARYTELRLSPLAMELLRDIDKETVDFVPNYDGYEQEPVV"
     gene            complement(501..2420)
                     /gene="gyrB"
                     /locus_tag="VEO00_00220"
     CDS             complement(501..2420)
                     /gene="gyrB"
                     /locus_tag="VEO00_00220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016906164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HYU56459.1"
                     /translation="MVRKPAEETYGSKDITVLEGLEAVRRRPGMYIGTTGSRGLHHLV
                     YEVVDNAIDEALTGFCSKILVRLRADGGVTVVDDGRGIPVKPIPKIKDRRPAVEVVLT
                     TLHAGGKFGGGGYQVSGGLHGVGVSVVNALSARLEVLVARDGYQWSQAYERGQRKHAL
                     RKGKAARKTGTSVTFWPDPEVFEETAFKAEVLTERLREMSFLVRGVEIGLVDEREEPP
                     AKTVYRATGGIADFVKFLNQGKDVLHAKPIHFEGTKEGAEVEVAMQWNSGYAESIHTF
                     ANTINTHEGGMHEEGFKKALTNVVNRYARTKGLLKEKEENLIGEDIREGLTAIVSVKL
                     REPQFEGQTKTKLGNTEMRSFVETTVNQHFATWLEEHPGDAKRVVLKSSQAARARLAA
                     RQARDLTRRKGLLGSSSLPGKLADCQSTDTETTELFIVEGDSAGGSAKQARERAFQAI
                     LPIRGKILNVEKARLHKILENVEIQALISAIGTGIGEDFDVSKARYGKVAILADADVD
                     GAHIRTLLLTFLFRHMQALIDAGRVYVAQPPLYRVKQDGRYVYILDEKQLERVRTENG
                     HKRIEISRFKGLGEMNAEQLWESTMNPETRVLKRVTLEDAAAADRMFSILMGEDVEAR
                     RAFIQANAKEAFIDA"
     gene            complement(2550..2903)
                     /locus_tag="VEO00_00225"
     CDS             complement(2550..2903)
                     /locus_tag="VEO00_00225"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HYU56460.1"
                     /translation="MSRPPDPRLHRRERSPEAHRLGEILDGLLRERPLVRGLPLGRLA
                     RHWEAVVGPKLAEVTAPVRLEEGVLWVVAETQGWAAQVRFLAGDVARRANEALGSDRI
                     AKVQVSVGKSGREAR"
     gene            complement(2887..3987)
                     /locus_tag="VEO00_00230"
     CDS             complement(2887..3987)
                     /locus_tag="VEO00_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HYU56461.1"
                     /translation="MRLSWVELRDFRNHRETRLEVPPGVSAVVGDNGQGKTNLLEGVY
                     YLFSLESPRASADLPLIRAGASSAFVRGEVETGTGRVLVEVEVRPSGANRVQVDRSPV
                     RRKRDVRQKVRAVFFMPDDIAVVQGEPDERRRLMDAVVVSLWPARDAGRRAYDKALRQ
                     RNRLLKDWEGGGTPAGMAAWDAELVEAGAALTRDRARAVERIREAASDAFAELSGGDR
                     LDVAYRPSVEPSADPEDLDALAARFRERLAARREDELVRRTTLVGPHRDELDLAVRTL
                     AARSFASHGEALVGALCLRLAQARAVRDEVGEPPVLFLDDPFSGLDPVRRRRLAGTVS
                     GLGQVLLSVPDAGQVPAGARTFRVEEGRVAAA"
     gene            complement(4001..5110)
                     /gene="dnaN"
                     /locus_tag="VEO00_00235"
     CDS             complement(4001..5110)
                     /gene="dnaN"
                     /locus_tag="VEO00_00235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HYU56462.1"
                     /translation="MKFRCDRDVLADALQTVQRGVATRPGIPALTGVLMRVEGGSLNL
                     STTDLEVSVEFRVEVRGDEEGDALVPARLVADIVRKLDPALVEFTARGGQATISCAAY
                     EGQIRCLQAEDFPARREPGGTEVLVAAAEFAEAVKQVGRAASKDEARPILTGVLLEAN
                     REGLNLVATDSYRLAIREVTAKGAGETRALVPERALTEAGRAAEGDEKGEVRILLEDS
                     QATFAVGALTLTSRLIEGEFPNYRQLTPEGYESHLVVPRAQLLDAVSRVGLLARENSP
                     IRLEFNALGVRLSSSSPDLGGAVEVVEAQYQGEEMTIAFNPGYLMDGLQAATGEKATL
                     EVKDGIKPVVVRGEGSGFLYLVMPVRLPESVRAVP"
     gene            complement(5344..>6269)
                     /gene="dnaA"
                     /locus_tag="VEO00_00240"
     CDS             complement(5344..>6269)
                     /gene="dnaA"
                     /locus_tag="VEO00_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HYU56463.1"
                     /translation="NPLFIYGGVGLGKTHLLHAIAGHMVRLHPGMRVKYVTSESFVTE
                     FIRAVRERRGYQFQRTYRDVDVLLVDDIQFLARAEETQTEFFHTINELHVAGHQICIA
                     SDRAPQELSALEERLRSRFRAGLCVDVQPPDLETRIAILQMKAIRDGIEVPDSVTHFI
                     ASRIEHNIRELEGALLRVAAFASLQSTQIDLALAERALEDLLPESEQEISPALIVDET
                     AAYFGLSREDLLSKSRSRQLTIARHMAMYLQREFTGLSLIKIGEWFNRDHTTALYGID
                     KIAKLMSARGSVYRQVQELTKKIRARSRGGD"
BASE COUNT          875 a         2331 c         2096 g          967 t
ORIGIN      
        1 ggaccacggg ctcctgctcg tacccgtcgt agttgggaac gaagtcgacg gtctccttgt
       61 cgatgtcgcg cagcagctcc atcgccagcg gcgacagccg cagctccgtg tagcgcgcgg
      121 ccgcgggcgg gtcgccgtcg acgttgccga agttgccctg cgggtcgatc agcgggtagc
      181 ggagcgagaa gtcctgcgcc atgcgtacca tggcgtcgta gacgggctgg tcgccgtgcg
      241 ggtggtactt cttgatcacg tcgcccaccg ccgcggcgga cttgcggcgt ggccggtcgg
      301 ggctcagccc cccctccatc atcgagaaga ggatccggcg gtggaccggc ttcagcccgt
      361 cccgggcgtc gggcagcgcc cgcgacacga tcaccgacat cgcgtagtcg atgaaggagg
      421 actggatctc ctcctcgatg acgacgtcgt cgatgcggtc gaacagcgtg gccatctacg
      481 ggctcatcct gcctcggcca tcaggcgtcg atgaacgctt ccttggcgtt cgcctggatg
      541 aacgcgcggc gagcctcgac gtcctcgccc atcaggatgc tgaacatgcg atcggccgcc
      601 gcggcgtcct cgagggtgac ccgcttcagg acgcgcgtct ccgggttcat ggtggactcc
      661 cacagctgct cggcgttcat ctcgccgagc cccttgaacc ggctgatctc gatccgcttg
      721 tgcccgttct ccgtccgcac ccgctcgagc tgcttctcgt cgaggatgta gacgtacctg
      781 ccgtcctgct tcacgcggta cagcggcggc tgggcgacgt acaccctgcc ggcgtcgatc
      841 agcgcctgca tgtgccggaa caggaacgtc agaagcagcg tccggatgtg cgccccgtcg
      901 acgtcggcgt cggcgaggat cgccaccttg ccgtagcgcg ccttcgacac gtcgaagtcc
      961 tcgccgatcc cggtgccgat cgcggagatc agcgcctgga tctcgacgtt ctccaggatc
     1021 ttgtgcagcc gcgccttctc gacgttcagg atcttgccgc ggatcggaag gatggcctgg
     1081 aacgcgcgct cgcgggcctg cttcgccgag ccgccggccg agtcgccctc gacgatgaac
     1141 agctcggtgg tctcggtgtc ggtggactgg cagtcggcga gcttgcccgg gagcgaggag
     1201 gaccccagca ggcccttgcg ccgcgtgagg tcgcgggcct gccgcgccgc caggcgcgcc
     1261 ctcgccgcct ggctcgactt cagcacgacc cgcttcgcgt cgccagggtg ctcctcgagc
     1321 cacgtggcga agtgctggtt gaccgtggtc tcgacgaacg agcgcatctc ggtgttcccg
     1381 agcttcgtct tcgtctgtcc ctcgaactgc ggctcgcgca gcttgaccga cacgatggcc
     1441 gtcaggccct cccggatgtc ctcgccgatc aggttctctt ccttctcctt cagcaggccc
     1501 ttggtccgcg cgtagcggtt cacgacgttg gtcagggcct tcttgaagcc ctcttcgtgc
     1561 atcccgccct catgcgtgtt gatcgtgttg gcgaacgtgt ggatcgactc cgcgtagccg
     1621 ctgttccact gcatcgccac ctcgacttcg gcgccctcct tcgtcccctc gaagtggatc
     1681 ggcttggcgt ggaggacatc cttgccctgg ttcaggaact tgacgaagtc ggcgatgccg
     1741 ccggtggcgc ggtagacggt cttcgccggc ggctcctccc gctcgtcgac caggccgatc
     1801 tcgacgccgc ggaccaggaa cgacatctcg cgcaggcgct cggtgaggac ctcggccttg
     1861 aacgccgtct cctcgaagac ctcggggtcg ggccagaacg tgacgctcgt gccggtcttc
     1921 ctggccgcct tgcccttgcg cagggcgtgc ttgcgctgcc cgcgctcgta ggcctgcgac
     1981 cactgatagc cgtcgcgcgc cacgaggacc tcgagccggg ccgacagcgc gttcaccacg
     2041 gacacgccga cgccgtgcag gccgccggac acctggtagc ccccgccgcc gaacttgccg
     2101 ccggcgtgca gggtcgtcag cacgacctcg accgccgggc gacggtcctt gatcttcggg
     2161 atcggcttga ccgggatgcc gcggccgtcg tcgacgaccg tcacgccgcc gtccgcccgc
     2221 agccggacca ggatcttgct gcagaagccg gtcagggcct cgtcgatggc gttgtcgacc
     2281 acctcataga cgaggtgatg gaggccgcgg gagcccgtgg tcccgatgta catcccgggc
     2341 cggcggcgga cggcctcgag cccctcgagg acggtgatgt ccttggaccc ataggtctct
     2401 tccgccggct ttcggaccac gctacctccg tccgggcccg gcgcatgcca cccatgcggc
     2461 ggcccgggaa cgctgcacct tcgaggggga ttccgggggg gaacctgtac ccgaagtata
     2521 ccagccggga ccgacggacc ggggcattct caccgagctt cgcggcccga tttacccacg
     2581 ctgacctgca cttttgcgat ccggtcgctc ccgagcgcct cgttcgcgcg gcgcgccacg
     2641 tcccccgcca ggaagcgcac ctgcgcggcc catccctggg tctcggcgac cacccaaagc
     2701 acgccctcct ccagccggac cggggccgtc acctcggcca gcttcgggcc gacgacggcc
     2761 tcccagtgcc gcgccagacg cccgaggggc agcccgcgaa cgaggggccg ctcacggagc
     2821 aggccgtcca ggatctcccc gagccggtgc gcctcggggc tgcgctcccg gcggtgcagc
     2881 cgggggtcag gcggccgcga cacggccctc ctccacgcgg aacgtgcgcg cccccgccgg
     2941 cacctggccg gcgtcgggca ccgagagcag cacctgtccc aggccggaca cggttcccgc
     3001 caggcgccgg cggcgcaccg ggtcgaggcc ggagaacggg tcgtcgagga acagcaccgg
     3061 cggctcgccg acctcgtcgc gcaccgcgcg ggcctgggcc aggcgcaggc agagcgcgcc
     3121 gaccagcgcc tccccgtgcg aggcgaacga gcgggccgca agggttcgca cggccaggtc
     3181 cagttcgtct cgatgcggtc cgaccagcgt ggtgcggcgg accagctcgt cctcccgccg
     3241 ggcggccagg cgctcccgga agcgcgccgc cagcgcgtcg aggtcctcgg ggtccgccga
     3301 cggctcgacg gaggggcggt aggcgacatc cagccggtcg ccgccggaca gctcggcgaa
     3361 ggcgtccgag gcggcctccc ggatccgctc caccgcccgg gcccggtccc gggtcagcgc
     3421 ggccccggcc tccacgagct ccgcgtccca cgccgccatc ccggccggcg ttccgccccc
     3481 ctcccagtcc ttcagcagcc ggttgcgctg gcgcagcgcc ttgtcgtacg cgcggcgccc
     3541 ggcatcacgg gcgggccaga gcgacacgac caccgcgtcc atcaatcgcc gccgctcgtc
     3601 cggctcgccc tgcaccaccg cgatgtcgtc gggcatgaag aacaccgccc ggaccttctg
     3661 ccgcacgtcc cgcttgcggc gcaccggcga ccggtccacc tgcacccggt tcgcccccga
     3721 gggccggacc tccacctcga ccagcacccg gccggtgccg gtctccacct cgccgcggac
     3781 gaacgccgac gacgcgcccg cgcggatcag cggcaggtcg gcggaggcgc gcggcgactc
     3841 gagcgagaac aggtagtaca ccccctccag caggttcgtc ttcccctggc cgttgtcgcc
     3901 gaccacggcc gagacgcccg gcgggacctc caggcgggtc tcgcggtggt tgcggaagtc
     3961 gcgcaactcg acccaggaga gccgcacgac accgccggcc ctatgggacg gcccggacgc
     4021 tctccggcag ccgcaccggc atgacgaggt acaggaatcc gctcccctcg ccccgcacca
     4081 cgaccggctt gatgccgtcc ttcacctcga gggtggcctt ctcgccggtg gcggcctgca
     4141 gcccatccat caggtacccg gggttgaagg cgatcgtcat ctcctcgccc tggtactggg
     4201 cctcgaccac ctcgaccgcg ccgccgaggt cgggcgagga cgacgacagc cggaccccga
     4261 gcgcgttgaa ctccagccgg atcggcgagt tctcgcgcgc cagcaggccg acacgggaca
     4321 ccgcgtcgag gagctgcgcc cgcggcacca ccaggtggct ctcgtagccc tcgggcgtca
     4381 gctgccggta gttcgggaac tcgccctcga tcagccggga cgtgagcgtc agcgcgccga
     4441 cggcgaacgt ggcctggctg tcctcgagca ggatccggac ctcgcccttc tcgtccccct
     4501 ccgcggcgcg ccccgcctcg gtcagcgccc gctccgggac cagggcccgc gtctcgccgg
     4561 cgcccttcgc ggtgacctcg cggatcgcca ggcggtacga gtcggtcgcc acgaggttca
     4621 gcccctcgcg gttcgcctcg agcagcaccc cggtcaggat cgggcgagcc tcgtccttcg
     4681 acgccgcacg gccgacctgc ttcaccgcct cggcgaactc ggcggccgcc accaggacct
     4741 cggtcccgcc cggctcacgc cgggccggga agtcctcggc ctgcaggcag cggatctggc
     4801 cctcgtaggc agcgcaggag atcgtggcct gcccgccgcg tgccgtgaac tccacgagcg
     4861 ccgggtcgag cttgcgcacg atgtcggcga ccagccgcgc tgggaccaag gcgtccccct
     4921 cctcatcgcc ccgcacctcg acgcggaact ccaccgatac ctcgagatcg gtggtcgaca
     4981 ggttcagcga ccccccctcc acccgcatca gcactccggt cagcgcgggg atgcccgggc
     5041 gggtcgccac ccctcgctgc acggtctgca gggcatcggc cagaacgtct cggtcgcacc
     5101 ggaacttcac aggtcacctc caacgatccg atggatgtat gaaccagcag cagtagtagg
     5161 gagggtgagt gtgtggaaac gccccatccg agcagcgtag cggcgaggta ggacacgctc
     5221 ggatttccac accccgcacg ggcgctgtgg atgttcagcc cccggacggg gccggttcac
     5281 cggcccgaca ccatacccca ccgattcccc caatcggatt cccccatgcc tccacgcgtg
     5341 cgctcagtcc ccgccccggg acctcgcccg gatcttcttc gtcagctcct ggacctggcg
     5401 gtagacgctc cctcgggcgg acatcagctt cgcgatcttg tcgattccgt agagcgccgt
     5461 cgtgtggtcc cggttgaacc actccccgat cttgatcagc gacagcccgg tgaactcgcg
     5521 ctgcaagtac atcgccatgt gccgggcgat ggtcagctgc cggctgcggc tcttcgacag
     5581 caggtcctcg cgggagaggc cgaagtacgc ggcggtctcg tcgacgatca gcgccggcga
     5641 gatctcctgc tcgctctcgg gcagcaggtc ctccagcgct cgctcggcca gggcgaggtc
     5701 gatctgggtg ctctgcagcg acgcgaacgc cgcaacgcgc agcagcgcac cctcgagctc
     5761 gcggatgttg tgctcgatgc gggaggcgat gaagtgggtc acggagtcgg gcacctcgat
     5821 cccgtcgcgg atcgccttca tctgcaggat ggcgatgcgg gtctcgaggt ccgggggctg
     5881 cacgtccacg cagaggccgg cgcggaagcg gctgcgtagc cgctcctcga gcgccgagag
     5941 ctcctgcggc gcccggtccg aggcgatgca gatctggtgc ccggcgacgt gcagctcgtt
     6001 gatcgtgtgg aagaactcgg tctgggtctc ctcggcgcgc gccaggaact ggatgtcgtc
     6061 cacgagcagc acgtccacgt cgcggtacgt gcgctggaac tggtagccgc ggcgctcgcg
     6121 gacggcgcgg atgaactcgg tgacgaagga ctctgaggtc acgtacttca cgcgcatccc
     6181 ggggtgcagg cggaccatgt gcccggcaat ggcatgtaga aggtgggtct tccccagacc
     6241 gacgccgccg tagatgaaca gcgggttgt
//
LOCUS       DASPWO010000011         3403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_256111, whole genome shotgun sequence.
ACCESSION   DASPWO010000011 DASPWO010000000
VERSION     DASPWO010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256111"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..859)
                     /locus_tag="VEO00_00245"
     CDS             complement(59..859)
                     /locus_tag="VEO00_00245"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HYU56464.1"
                     /translation="MPESTRALSEQDLRDAVRAAGIDVPPSFHEVTGSTNADALELAR
                     AGAPEWTVVAAGFQEAGRGRLGRTWSSPPGTSILLSVLLRPALPPGSAPLLSLLVSRA
                     MADACAAAAGVAARLKWPNDLVVGERKLAGVLPESTVAAGRLEHVVVGVGLNVAQRPE
                     DFPPEVGESATSLALEGGGGDQPAIVEAFLRAAREAYRPAEAGFAETVVAAARERSAT
                     LGRRVRAVTVDGRRVEGVAADLGPDGALLIDADGRREAVGFGEVAHLE"
     gene            925..2685
                     /locus_tag="VEO00_00250"
     CDS             925..2685
                     /locus_tag="VEO00_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HYU56465.1"
                     /translation="MAEKVLIANRGEIAVRVMRTCRELGIPTVAVYSDADRDALFVEQ
                     ADEAHRLGPAPPSESYLNVPAILAVAKRSKATLVHPGYGFLAEDPDFARTVAGAGMRF
                     VGPPPEAMEGMGDKISARRTARRIGVPVVPGTDEPVSPEEALRIAEGVGFPLAVKAAY
                     GGGGRGMHIVRAPADLREAVERSAREATAYFGRPEVYLERFVEHGHHVEAQVVADLHG
                     AAFFLGERDCTLQRRYQKLVEESPSPIVDPGLRARIEESALALVKEVGYANAGTVEFL
                     VDEERNPYFLEVNARLQVEHPVTEMVTGLDLVACQIFAALGERLHLAPQPRGHAIECR
                     INAEDPYRDFLPGAGRIARFEPPGGPFVRVDAGFGEDRTIPGDYDSLLAKLVVWGEDR
                     ERARSRMLRALGEFHVEGVPTTIPFHRWVLQTTQFVDATADTRWVEHALAEAPLPAPK
                     GGPGETSPGPGPGPSVLITAEVKGRRVPVRLWGDGVRTPPAPPPSLAQRGAAGAGSGV
                     VSAPMQGTILQVLVEEGQAIAAGEVVCILEAMKMENHIAAAADGTVTELAVKAGQVVE
                     SGQTLAIVQANATPAGSSGS"
     gene            complement(2822..>3403)
                     /locus_tag="VEO00_00255"
     CDS             complement(2822..>3403)
                     /locus_tag="VEO00_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYU56466.1"
                     /translation="HSFGGFVALQNAVDFPGAAAGTIVSGGVPSMKFLDGIDRALRTF
                     EPEALRGQVTAAWERERTAESHDDVASLLHDQLPFHFADPLDPRIAEYEARAAGARYT
                     PAVLRAFGEGPSGAIEVEDRLDGIAQPVLVIAGRHDRVCPVEAAEVMAHLIPDAELAV
                     MERSGHMPFVEQQDRYVEAVRSFLDRRVATAPR"
BASE COUNT          451 a         1267 c         1288 g          397 t
ORIGIN      
        1 gagggaacgg catggccggg acgctaccgc gcgcgccgcc gggcggcaac gcgccccgct
       61 actcgaggtg ggccacctcg ccgaagccga ccgcctcccg tctcccatcg gcgtcgatca
      121 gcagcgcgcc gtcgggaccg aggtcggcgg cgacgccctc gaccctgcgg ccgtcgacgg
      181 tcaccgcccg tacccgccgc ccgagggtgg ccgagcgttc gcgtgccgcc gccacgaccg
      241 tctcggcgaa cccggcctcg gccggccggt acgcctcgcg cgccgcgcgc aggaaggcct
      301 cgacgatcgc cggctgatcc cccccgccgc cctcgagcgc caggctcgtg gcgctctcgc
      361 cgacctccgg cgggaagtcc tccgggcgct gggcgacgtt cagccccacg cccaccacga
      421 cgtgctcgag cctcccggcg gcgacggtgc tctccggcag gacgccggcc agcttgcgct
      481 ccccgaccac caggtcgttg ggccacttca ggcgggccgc gacgcccgcg gccgccgcgc
      541 acgcgtcggc catcgcgcgg gagaccagca gggagaggag cggcgccgag ccggggggga
      601 gtgccggccg gagcagcacc gagagcagga tcgaggtccc cggaggcgac gaccaggtcc
      661 gcccgaggcg cccgcggccg gcttcctgga agccggcggc gaccacggtc cactccgggg
      721 cgccggcccg cgccagctcg agggcgtcgg cgttcgtgga cccggtgacc tcgtggaacg
      781 acggcgggac gtcgatcccg gcggcgcgca cggcatcccg caggtcctgc tcgctcagcg
      841 cccgcgtaga ctccggcatc ggaggagcag tatcgcaagc gagagcgagc gcgggcgagc
      901 gggcagggag ggtggcggga tcggatggcg gagaaggtcc tgatcgccaa ccggggcgag
      961 atcgcggtgc gcgtcatgcg gacgtgccgc gagctcggga tccccactgt ggccgtgtac
     1021 tccgacgccg accgtgacgc gctgttcgtc gagcaggccg acgaggccca ccgcctcggc
     1081 ccggccccgc cgtccgagtc ttacctgaac gtcccggcca tcctcgccgt ggcgaagcga
     1141 tcgaaggcca ccctggtcca cccggggtac ggcttcctgg cggaggaccc cgacttcgcc
     1201 cggaccgtgg cgggcgccgg gatgcgcttc gtcgggccgc cgccggaggc gatggaaggg
     1261 atgggcgaca agatcagcgc ccggcgcacg gcgcggcgga tcggggtgcc ggtggtcccc
     1321 ggcaccgacg agccggtctc ccccgaggag gccctccgga tcgcggaggg cgtcggcttc
     1381 ccgctcgcgg tcaaggccgc ctacggtggc ggggggcgcg ggatgcacat cgtgcgggcc
     1441 cccgcggacc tgcgcgaggc cgtggagcgg tcggcacgcg aggcgaccgc gtacttcggc
     1501 cggccggagg tgtacctgga gcgcttcgtc gagcacggcc accacgtcga ggcccaggtg
     1561 gtcgccgacc tccacggcgc cgcgttcttc ctcggcgagc gcgactgcac gctgcagcgc
     1621 cgctaccaga agctggtcga ggagagcccg tccccgatcg tcgacccggg cctgcgcgcg
     1681 cggatcgagg agtccgccct ggcgctggtg aaggaagtcg gctacgcgaa tgccgggacc
     1741 gtcgagttcc tggtggacga ggagcgcaac ccctacttcc tggaggtgaa cgcccggctc
     1801 caggtcgaac acccggtgac cgagatggtc accgggctcg acctggtggc ctgccagatc
     1861 ttcgcggcgc tcggcgaacg gctccacctg gctccgcagc cgcgcggcca cgccatcgag
     1921 tgccggatca acgccgagga cccgtaccgc gacttcctgc ccggagcggg gcggatcgcc
     1981 cgcttcgagc cgcccggcgg gcccttcgtc cgcgtggacg ccggcttcgg cgaggaccgg
     2041 acgatccccg gcgactacga ctcgctgttg gcgaagctcg tggtctgggg cgaggaccgc
     2101 gagcgagccc ggtcccggat gctccgcgcc ctcggcgagt tccacgtcga gggcgtcccg
     2161 accacgatcc cgttccaccg ctgggtgctg cagaccacgc agttcgtgga cgcgaccgcc
     2221 gacacgcggt gggtggagca cgcgctggcc gaggccccgc tccccgcgcc caagggcggc
     2281 cccggggaga ccagcccggg gccgggtccc ggcccgtccg tgctgatcac cgccgaggtg
     2341 aaggggcggc gcgtccccgt gcggctgtgg ggcgacgggg tacgcacccc gccggcgccc
     2401 ccgccctcgc tcgcccagcg cggggccgcc ggcgccggga gcggcgtggt gtcggcgccg
     2461 atgcagggca cgatcctgca ggtcctggtg gaggagggcc aggcgatcgc ggccggcgag
     2521 gtggtctgca tcctcgaggc gatgaagatg gagaaccaca tcgcggcggc ggcggacggc
     2581 acggtcaccg agctcgccgt gaaggcgggc caggtcgtcg agagcggaca gaccctggcg
     2641 atcgtccagg cgaacgccac cccggccgga tcgtccggtt cctgacggag tgcccgggac
     2701 cgggccgaca tcctcccccg ttcgtgcagg gggggccggg gcgagctgaa cggcggggcg
     2761 aacaccgaca cctgcaacgg cgaggtcgag tccggcgaca ccgccaccga gtgcgaggcg
     2821 gtcaccgggg tgccgtggcc acccggcgat cgaggaacga gcggaccgcc tccacgtagc
     2881 ggtcctgctg ctcgacgaac ggcatgtgtc cgctgcgctc catgaccgcg agctcggcgt
     2941 cggggatcag gtgggccatc acctcggcgg cctcgaccgg gcagacccgg tcgtgacggc
     3001 cggcgatcac gagcaccggc tgggcgatcc cgtccagccg gtcctcgacc tcgatcgctc
     3061 ccgacggtcc ctccccgaac gcgcgcagca ccgccggcgt gtagcgggcg ccggcggcgc
     3121 gcgcctcgta ctcggcgatg cgcgggtcga gcggatcggc gaagtggaac ggcagctggt
     3181 cgtgcagcag ggacgcgacg tcgtcgtggc tctccgcggt gcgctcccgc tcccaggccg
     3241 cggtgacctg gccgcggagc gcctccggct cgaacgtccg cagcgcccgg tcgatcccgt
     3301 ccaggaactt catcgagggg acgccgccgc tgacgatcgt cccggcggcg gcgcccggga
     3361 agtccaccgc gttctgcagg gcgacgaacc cgccgaacga gtg
//
LOCUS       DASPWO010000012         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1189864, whole genome shotgun sequence.
ACCESSION   DASPWO010000012 DASPWO010000000
VERSION     DASPWO010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189864"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VEO00_00260"
     CDS             <1..556
                     /locus_tag="VEO00_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56467.1"
                     /translation="LQSYCIRDKGFKTSKRPVAVPEDAPETIRRMGEVAALVGVGPMF
                     TFPGALAEHVGRTLARSLPEVTVVNGGDTYAVTRRPSKLTVYRREGQALSVVIRPEQG
                     PAGVYTTEGSLLTAADSADAVVVVADSCVLADAVAAAVLAILSHPRSFAQALRLLRTT
                     AGVRGGIVVRDERIGLAGHVELAA"
     gene            570..2708
                     /gene="ligA"
                     /locus_tag="VEO00_00265"
     CDS             570..2708
                     /gene="ligA"
                     /locus_tag="VEO00_00265"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HYU56468.1"
                     /translation="MTPRGVRIGPPPGGRRRTAARARGGGEQRARQVDAAQERDPALE
                     RRLSAARVRVEELRELINHHRYRYHVLDDPEVSDAEYDELIRELETLEAEFPELVTPD
                     SPTQLVGAPPAELFRPVRHRARMLSLDNAFSPEELAAWVRRVEREVGRDVGYVCELKI
                     DGVAIALKYENGRFVEGSTRGDGEVGEDVTPNLRTIEEIPNRLKGSGHPRVLEVRGEV
                     YLPVKAFERINEELLADGGRTFANPRNAAAGTLRQKDPAVTASRPLEVWCHGVLFAEG
                     ARFASHSEVLAKLQSWGLRVNPDTRPAKDLDAVLAYCAHWQEHRHSVDYEIDGVVVKV
                     DSIGQQEELGATSKAPRWAIAYKFPPEERTTVLRKIDVHVGRTGAVTPFAFLEPVRVG
                     GVTVGLATLHNEDEVHRKDVRNGDTVIVRRAGDVIPEVVGPVPAKRPRGARVWHMPKN
                     CPSCGTPLVRPEGEAVWRCPNKRGCPSQAIESLFHFAGRGAMDVEGLGYKTVMALIDK
                     GFLSDFADVYALSPEKLSELPGFKDKAITNLMRAVEASKDRPLWRLLVGLSIRHVGGH
                     VAQVLAREFGTLDRLQAAELDELGAVEGIGPEIARSVYEWFRDPENLRLLVRLRTAGV
                     RTDEPVTEAAEGPLAGTTIVLTGGLQSMSRDEATAAAEAAGARVASSVSAKTSFVVGG
                     ENPGTKYDKAVSLGVEIVDEREFLKRLGRG"
     gene            2713..3675
                     /gene="ligD"
                     /locus_tag="VEO00_00270"
     CDS             2713..3675
                     /gene="ligD"
                     /locus_tag="VEO00_00270"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HYU56469.1"
                     /translation="MAESIAGVPLTNLDQPLFEGAGATKRDLVEYLDAVRDRIIPALR
                     DRLLSVIRAVRGQQPFMQKNVPAYAPDFVKTERLWADAPKRYVAYAICNDRRTLLWFA
                     NQRAVEFHPSLVRADRIDRVTHLVLDLDPPSADGFDQAVRVAHLVRRALADAGLEGAA
                     KTSGAKGVHVFVPIDRRASMEDAAAATRAIARRAEALDPELATTAFMKEDRQGKVFVD
                     STRAGGATVVSAYSPRARAGVPVSFPVDWGDLDRVAPSDFTIHTVPKLVAERDPWIEL
                     MPKAQRLPRALIEEGHEIPLGRVEAMHEGRRRARARRRGDKPER"
     gene            3754..>3938
                     /gene="gatC"
                     /locus_tag="VEO00_00275"
     CDS             3754..>3938
                     /gene="gatC"
                     /locus_tag="VEO00_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006037440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HYU56470.1"
                     /translation="MPVEIDIDHVARLARLALTDEERERFHGQLGAILEHAERVTKVA
                     VEDVPPTAHPLPRANVFR"
BASE COUNT          564 a         1362 c         1474 g          538 t
ORIGIN      
        1 gctgcagtcc tactgcatcc gcgacaaggg cttcaagacc agcaagcgtc cggtggccgt
       61 gccggaggac gcgccggaga cgatccggcg gatgggtgag gtggccgcgc tggtcggcgt
      121 ggggccgatg ttcaccttcc ccggcgccct ggccgagcat gtcggccgga cgctggcccg
      181 ctcgctcccc gaggtgaccg tggtgaacgg cggggacacg tacgcggtga cccgacggcc
      241 gtcgaagctc accgtctacc gccgtgaggg gcaggcgctg tcggtcgtca tccggccgga
      301 gcagggtccg gccggcgtct ataccaccga ggggagcctg ctcacggcgg cggactcagc
      361 ggacgccgtc gtggtggtgg ccgactcctg cgtcctggcc gacgcggtgg ccgcggcggt
      421 cctggccata ctcagccatc cccgctcgtt cgcccaggcg ctgcggctgt tgcggaccac
      481 ggccggcgtg cgcggcggga tcgtcgtccg ggacgagcgg atcggcctgg ccggtcacgt
      541 ggagcttgca gcgtgatcga aggatcagca tgactccgcg tggtgttcgg atcgggccac
      601 cgcccggcgg ccggcggcga acggctgccc gggcgcgcgg cggcggggag cagcgggccc
      661 gccaggtcga cgccgcgcag gagcgcgatc cggcgctcga gcggcggctg tcggcggcgc
      721 gggtccgggt ggaggagctc cgcgagttga tcaaccacca ccggtaccgc taccacgtcc
      781 tggacgaccc cgaggtctcc gacgccgagt acgacgagct gatccgggag ctcgagacgc
      841 tggaggccga gtttccggag ctggtcacgc cggattcgcc cacccagctc gtcggcgcgc
      901 cgccggcgga gctgttccgg cccgtgcgcc atcgcgcacg gatgctgtcg ctcgacaacg
      961 cgttctcccc ggaggagctc gctgcctggg tgcgtcgcgt cgagcgcgag gtgggaagag
     1021 acgtcggcta cgtctgcgag ctgaagatcg atggggtggc catcgcgctg aagtacgaga
     1081 acggccgctt cgtcgagggc tccacgcggg gcgacggcga ggtcggcgag gacgtcacgc
     1141 ccaacctgcg cacgatcgag gagatcccga accggctgaa gggctcgggc cacccccggg
     1201 tcctggaggt ccgcggcgag gtctacctgc cggtgaaggc cttcgagcgg atcaacgagg
     1261 agctcctggc ggacggtgga cggacgttcg ccaacccacg gaacgcggcg gccgggaccc
     1321 tgcgccagaa ggaccccgcc gtcaccgcct cccggccgct ggaggtgtgg tgccacggcg
     1381 tgctcttcgc cgagggcgcc aggttcgcga gccactccga ggtgctggcg aagctccagt
     1441 cgtggggcct gcgcgtcaac cccgacacca ggccggcgaa ggaccttgac gcggtgctcg
     1501 cctactgcgc gcactggcag gagcaccggc actcggtcga ctacgagatc gacggcgtgg
     1561 tggtgaaggt cgactcgatc ggacagcagg aggagcttgg ggcgacgagc aaggcgccgc
     1621 gctgggcgat cgcctacaag ttcccgcccg aggagcggac cacggtgctg cgcaagatcg
     1681 acgtccacgt cggtcgcacc ggcgccgtga ccccgttcgc ctttctcgag ccggtgcgcg
     1741 tcggcggcgt cacggtcggg ctcgcgacgc tgcacaacga ggacgaggtg caccgcaagg
     1801 acgtccggaa cggcgacacg gtgatcgtcc gccgggccgg cgacgtgatc ccggaggtcg
     1861 tcggccccgt cccggccaag cgcccgaggg gggcgcgcgt ctggcacatg ccgaagaact
     1921 gtccctcctg cgggacgccg ctcgtccgcc cggagggcga ggcggtgtgg cgctgtccga
     1981 acaagcgcgg ctgtccatcc caagcgatcg aatcgctgtt ccacttcgcc gggcgcgggg
     2041 cgatggacgt ggaggggctc gggtacaaga ccgtcatggc cctgatcgac aaggggttcc
     2101 tgagcgattt cgcggacgtc tacgcgttgt cgccggagaa gctgtcggag ctgccaggct
     2161 tcaaggacaa ggcgatcacg aatctgatgc gcgccgtcga ggcgtcgaag gaccggccgc
     2221 tctggcgctt gctggtcggc ttgtcgatcc gtcacgtcgg cgggcacgtg gcgcaggttc
     2281 tggcccgaga gttcggcacg ctcgatcggc tgcaggccgc cgagctcgac gagctcggag
     2341 ccgtcgaggg gatcggcccc gagatcgccc gcagcgtgta cgagtggttc cgcgacccgg
     2401 agaacctccg gctgctcgtc cgcctgcgca cggccggcgt gcgcaccgac gagccggtga
     2461 cggaggcggc ggagggcccg ctcgccggga ccacgatcgt cctgaccggt gggctccagt
     2521 cgatgtcccg cgacgaggcc accgccgccg ccgaggcggc cggcgcccgc gtcgcgtcga
     2581 gcgtcagcgc gaagacctcc ttcgtggtcg gcggggagaa cccgggcacg aagtacgaca
     2641 aggccgtctc gctcggcgtc gagatcgtcg acgagcgcga gttcctgaag cgcctggggc
     2701 gcgggtagcc ctgtggccga gtcgatcgcc ggcgtgccgc tgacgaacct cgatcaaccg
     2761 ctgttcgagg gtgccggcgc gacgaagcgc gacctggtcg agtacctcga cgcggtgcgc
     2821 gaccggatca tccccgcgct gcgggaccgg ctgctgtcgg tgatccgggc ggtgcgaggc
     2881 cagcagccgt tcatgcagaa gaatgtgccg gcgtacgcgc ccgacttcgt gaagaccgag
     2941 cgtctgtggg cggacgcgcc caagcggtat gtggcctacg cgatctgcaa cgaccgccgc
     3001 acgctcctct ggttcgccaa ccagcgagcg gtcgagtttc atccctcgct cgtccgtgcc
     3061 gaccggatcg accgcgtcac ccatctggtc ctcgacctcg acccaccctc ggccgacggc
     3121 ttcgaccagg ccgtgcgcgt ggcgcacctg gtgcgccggg cgctggccga cgcgggcctc
     3181 gagggggccg cgaagacgag cggcgcgaag ggggtgcacg tcttcgtccc gatcgaccgg
     3241 cgggcctcga tggaggatgc cgccgcggcc acccgcgcca tcgcccggcg cgccgaggcg
     3301 ctcgacccgg agctcgccac gaccgcgttc atgaaggagg accggcaggg gaaggtcttc
     3361 gtcgattcga cgcgcgcggg gggtgcgacg gtcgtttcgg cctacagccc gcgtgcccgc
     3421 gccggcgtgc cggtgtcgtt cccggtcgac tggggtgacc tcgaccgggt cgcgccatcc
     3481 gacttcacga tccacacggt cccgaagctc gtggccgagc gcgacccgtg gatcgagctc
     3541 atgccgaagg cgcagcgcct cccgcgggcg ctgatcgagg agggccacga gatcccgctg
     3601 gggcgcgtcg aggcgatgca cgagggccgg cgccgcgccc gggcccgccg ccgcggcgac
     3661 aagcccgagc gctgatgctc cgcagttccc atcaggtagt gatccctacg ggcgcgaaga
     3721 gccgggggcg gggcagcaac tagactcggt cgtatgccgg tcgagatcga catcgaccac
     3781 gtcgcgcggc tcgcgcggct cgcgctgacc gacgaggagc gcgagcggtt ccacggccag
     3841 ctcggcgcga tcctcgagca cgccgaacgg gtcacgaagg tcgcagtgga ggacgtgccc
     3901 cccaccgccc acccgctgcc ccgcgcgaac gtcttccg
//
LOCUS       DASPWO010000013         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1801, whole genome shotgun sequence.
ACCESSION   DASPWO010000013 DASPWO010000000
VERSION     DASPWO010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VEO00_00280"
     CDS             complement(<1..837)
                     /locus_tag="VEO00_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56471.1"
                     /translation="MITAASLSDLRLRRPARRVLIVLTCLASAVTYAAMRASVTDVIL
                     SSYARTCARTGLRADEWLCGTTRAQVLAAFVVGSLLVGAGIALPCVVLALSGRRAGAF
                     APLAVALVTTLFSLRQTSYLPTQLFGIPEGWPATLGRDPLPSFWQAHPGLGFVADLVI
                     VSLPVLAFLQWGGRSSVRRERPSRRGATFASLIALVAIGVIGWISGWLVEMWGPGLVM
                     ATFAALLGTDRRWWPWVLPPVAVLLSLGPAMAVISTITQFTTLAWFRSVVPLVAIGLA
                     ASS"
     gene            complement(892..>2013)
                     /locus_tag="VEO00_00285"
     CDS             complement(892..>2013)
                     /locus_tag="VEO00_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HYU56472.1"
                     /translation="RLMPDPSALKVLAAQPGWAWAPVDKHTQEGEIYPGCQRSFAIRM
                     ARRAADAGIELRMAFELEWFQADADSDIDNPVHTGPAYGFPVMATVSDFARDLITTLE
                     DEGVGVEQFHPEYAMGQLEISVAPGDPVGAADTAVLVRQTIRAVAATYGMRPSFAPVV
                     IAGHVGNGGHVHFSLWRDGTNLFAGGDGPHGMTETGEAFVAGVLAELPALVAVGSPSV
                     PSYLRLIPSHWAGAFACWGRENREAAVRFITGMVGTRESAANTEVKCFDQSANPYLVV
                     GALIAAGLAGVEKGLRLPDEVTVDPGGLTDHELATRGIQRLPRSLEEALTYLEKSDLL
                     RDSLGPMLFGPFAAVRRAEAEAFADQEPDEIVAAHRWRY"
BASE COUNT          336 a          698 c          729 g          250 t
ORIGIN      
        1 ggacgacgcg gcgagcccga tggcgaccag gggcaccacg gaacggaacc acgcgagggt
       61 ggtgaactgc gtgatcgtgc tgatcacggc catcgccggc ccgagggaga gcagcaccgc
      121 gaccgggggc agcacccacg gccaccagcg gcggtcggtt ccgaggagag ccgcgaacgt
      181 cgccatgacg aggcccggcc cccacatctc gaccagccat ccggagatcc atccgatgac
      241 gccgatcgcg acgagggcga tgagcgaggc gaacgtggct ccccggcgcg acggacgctc
      301 ccggcggacg gagctccgac cgccccactg caagaaggcg agcaccggaa gggacacgat
      361 cacgaggtcc gcgacgaacc cgagtccggg atgggcctgc cagaacgagg gcaacgggtc
      421 tctgccgagg gtggccggcc acccctccgg gatcccgaag agttgggtgg ggagatacga
      481 agtctgtctc agcgagaaga gggtcgtgac gagggcgacc gcaagcggcg cgaacgcgcc
      541 ggcgcgacgt ccggaaagag cgagcaccac acaggggagg gcgatccccg cgccgaccag
      601 gagcgatccg acgacgaacg cggcgagcac ctgtgcgcgt gtcgtgccgc acaaccactc
      661 atcggcccgc aggccggttc gggcgcaggt tctggcgtag gacgagagga tcacgtccgt
      721 gaccgatgcg cgcatggcgg cgtacgtcac ggccgaggcg aggcacgtga gcacgatcag
      781 cacccggcgc gccggtcgcc ggagcctgag gtcggagagc gatgcggccg tgatcaccac
      841 ccagtgggta tcggtttccg tgcgaagcga ctgaacggcg ggccgctccg ctcagtagcg
      901 ccaccggtgg gcggcaacga tctcgtccgg ctcctgatcg gcgaaggcct ccgcctccgc
      961 tcggcggacg gccgcgaacg gcccgaacaa catcggcccc aaggagtcgc gcagcaggtc
     1021 ggacttctcc aggtacgtca aggcttcctc gagggatcga gggagccgtt ggatgcccct
     1081 cgtcgcgagc tcgtggtccg tcaggccgcc tgggtcgacg gtgacctcgt cgggcaggcg
     1141 caggcccttc tccacgccgg ccagccccgc cgcgatgagg gctccgacga ccaggtaggg
     1201 gttcgcgctc tgatcgaagc acttcacctc cgtgttggcc gcggactcac gtgtcccgac
     1261 catcccggtg atgaaccgaa cggcggcttc ccggttctcc cgtccccagc aggcgaaggc
     1321 gccggcccag tgcgacggga tcagccgaag gtagctgggg acgctgggcg agcccacggc
     1381 gacgagggcc ggaagctcgg cgagcacgcc ggcgacgaag gcctcgcccg tctcggtcat
     1441 gccgtgcggc ccgtcgccgc cggcgaacag gttcgtcccg tctcgccaca ggctgaagtg
     1501 gacgtgcccc ccgttcccga cgtgtccggc gatcaccacg ggtgcgaacg acgggcgcat
     1561 cccgtaggtc gcggccacgg cccggatcgt ctggcggaca agcacggcgg tgtcggcggc
     1621 gccgaccgga tcccccggcg cgacggagat ctcgagctgc cccatcgcgt actcgggatg
     1681 gaactgctcg acccccacgc cctcgtcctc gagcgtggtg atgaggtccc gggcgaagtc
     1741 cgagaccgtg gccatgacgg ggaacccgta ggccgggccg gtgtggacgg gattgtcgat
     1801 gtccgagtcg gcgtcggcct ggaaccactc gagctcgaag gccatccgga gctcgatccc
     1861 ggcgtcggcg gcccgcctcg ccatccggat cgcgaagctc cgctggcagc ccggatagat
     1921 ctcgccctcc tgggtgtgct tgtcgaccgg ggcccacgcc catccgggct gggcggcgag
     1981 caccttcagc gccgagggat ccggcatcag tcg
//
LOCUS       DASPWO010000014         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_680557, whole genome shotgun sequence.
ACCESSION   DASPWO010000014 DASPWO010000000
VERSION     DASPWO010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680557"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VEO00_00290"
     CDS             complement(<1..883)
                     /locus_tag="VEO00_00290"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYU56473.1"
                     /translation="MTEKSVVQTIHDTLFEEMQADDRVLVLGEDVGARGGVFRVTQGF
                     LDAFGEQRVVDTPLAESMIVGVAIGMALHGLLPVAEIQFADFIHPAFDQIVSEAARIR
                     YRSNGDFGCPLVIRAPWGGGVHGALYHSQSIEAFYGHVPGLKVVLPSTPHDAKGLLRA
                     AIRDPDPVLFLEHKKTYRLIKGEVPDEDYEVPIGVADVKREGEDLTCIAYGLMLHHCL
                     EAAQKLQEEDGVSVEVVDVRTIAPLDKETILHSVRKTGKAMVVYEDNRTYGAGAEIIA
                     TIAEEAMFDLDGPLVRFG"
     gene            complement(880..1923)
                     /locus_tag="VEO00_00295"
     CDS             complement(880..1923)
                     /locus_tag="VEO00_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HYU56474.1"
                     /translation="MTETGQQQSRHAALGLTDEDLRGMYRVMLTARLCDEAQFRLNRQ
                     GKAPFVVPVSGHEGAQVGSAWPFKPGTDIFVPYYRDMTICLVAGMTPRDVFLGVFGKR
                     DDPSSGGRQMPAHWGSRRLGIITGSSPIATQVLHAAGVAYAAKYRGEEAVVGCWFGDG
                     ATSEGDWHEALNFSGIHRLPVVWVCENNKYAISVPLSKQMAVKDVAMRAEGYGFEGVV
                     VDGNDVLGTYEAMARAVEKARGGGGPTLVEAKTYRFYPHTSDDDDRTYRSREEVEEAR
                     RNDPILLFGRYLVEQGLLPEDGPDAVRAEIKAQVDAAADEAWKAPDPEPDSALRHVFA
                     EPASESPEEADAR"
     gene            2136..2642
                     /locus_tag="VEO00_00300"
     CDS             2136..2642
                     /locus_tag="VEO00_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020605891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU56475.1"
                     /translation="MAEIREADLPRDLDAVERLWLEYLTWGNDGLQSRYGFRLPVREA
                     VEHDLATIAKFQPPDGRLLLAFERDVAVGTACMRRIGPDTAEIKRMYIQPSHRRAGVG
                     RALQDRLIAAARNAGYERVRLDSPDFMTAAHDLYRSSGFVEIGPYPESEIPDEYKPHW
                     LFMERRLT"
     gene            2727..>3154
                     /locus_tag="VEO00_00305"
     CDS             2727..>3154
                     /locus_tag="VEO00_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYU56476.1"
                     /translation="MAKLAARVTDGAEAGPDEPLAALGIDSLGMAELALAVEDEFGVR
                     LAAAPPERLRTVRDVARAVARLQGRAGGPRLPEGIGRLQDTARRAVGPVLTGWYRFHA
                     EGVEQIPRTGAAVIAANHGSLLDVPFLALACPRDIWFMAK"
BASE COUNT          455 a         1098 c         1114 g          487 t
ORIGIN      
        1 cgccgaaccg aacgagcggg ccgtccaggt cgaacatcgc ctcctcggcg atcgtggcga
       61 tgatctcggc ccccgcgccg taggtcctgt tgtcctcgta gacgaccatc gccttgccgg
      121 tcttgcgcac cgagtgaagg atcgtctcct tgtcgagcgg ggcgatcgtg cgcacgtcca
      181 ccacctcgac ggagaccccg tcctcctcct gcagcttctg ggcggcctcc aggcagtggt
      241 gcagcatcag cccgtaggcg atgcaggtca ggtcctcgcc ctcgcgcttg acgtcggcga
      301 ccccgatcgg gacctcgtag tcctcgtccg ggacctcgcc cttgatcagg cggtacgtct
      361 tcttgtgctc gaggaacagc acggggtccg ggtcgcggat cgcggcgcgg agcaggccct
      421 tcgcgtcgtg gggcgtggag gggagcacga ccttcaaccc ggggacgtgg ccgtagaacg
      481 cctcgatcga ctgcgaatgg tacagcgcgc cgtgcacgcc tccgccccac ggcgcccgga
      541 tcacgagcgg gcatccgaag tcgccgttcg accggtagcg gatgcgcgcc gcctcggaca
      601 cgatctggtc gaacgccggg tggatgaagt cggcgaactg gatctcggcc accggcagca
      661 ggccgtgcag cgccatcccg atggccacgc cgacgatcat cgactcggcg agcggcgtgt
      721 cgaccacccg ctgctcaccg aacgcgtcga ggaacccctg cgtcacgcgg aacaccccgc
      781 cgcgcgcccc gacgtcctcg ccgaggacca gcacgcggtc gtcggcctgc atctcctcga
      841 acagcgtgtc gtggatggtc tggacgacgc tcttctccgt catcgcgcgt ctgcttcctc
      901 gggggattcg ctcgccggct cggcgaagac gtggcgcaac gccgaatccg gctcggggtc
      961 gggggccttc caggcctcgt ccgcggcggc gtcgacctgc gccttgatct cggcgcggac
     1021 cgcgtcgggc ccatcctcgg ggaggagtcc ctgctccacc aggtagcgcc cgaacagcag
     1081 gatggggtcg ttccggcgcg cctcctcgac ctcctcccgc gaccggtagg tgcggtcgtc
     1141 gtcgtcggag gtgtgcgggt agaagcggta ggtcttggct tcgacgagcg tcggcccgcc
     1201 gccgccgcgg gccttctcca cggcccgggc catcgcctcg taggtgccga gaacgtcgtt
     1261 gccgtccacg acgacgccct cgaacccgta gccctccgcc cgcatcgcga cgtccttgac
     1321 ggccatctgc ttcgagagcg gcacggatat cgcgtacttg ttgttctcgc agacccacac
     1381 cacgggcagg cgatggatgc cggagaagtt cagtgcctcg tgccagtcgc cctcgctcgt
     1441 ggctccgtca ccgaaccagc acccgacgac cgcctcctcg ccgcggtact tcgcggcgta
     1501 ggcgacgccg gccgcgtgca gcacctgcgt ggcgatcggg gaggacccgg tgatgatccc
     1561 cagcctccgc gacccccaat gtgccggcat ctggcgcccg ccagaagagg ggtcgtcgcg
     1621 cttgccgaac acgccgagga acacgtcgcg cggcgtcatg ccggccacca ggcagatggt
     1681 catgtcgcgg tagtagggga cgaagatgtc ggtgccgggc ttgaagggcc aggccgaccc
     1741 gacctgagcg ccctcgtggc ccgaaaccgg caccacgaac ggggccttcc cctggcggtt
     1801 gagacggaac tgggcctcgt cgcacagccg cgccgtgagc atgacgcggt acatgccgcg
     1861 caggtcctcg tcggtgagcc cgagcgccgc gtggcggctc tgttgctgcc cggtctccgt
     1921 catgcgcgtc gccaggcgac cgcctcctcg cgagtgggga ccatcgcgat catagcgata
     1981 gagtatccga acggttaacg gcacgttgac ctggggtttt gggcgtccga aggatgcggt
     2041 gcgggggagt cattccgggg acgaatgtcg ccggggagtt cggggcggtg tacgcccgca
     2101 gatgagcggg cgtgcggtta gcctgaggcc ggccaatggc ggagatccga gaggccgatc
     2161 tgccgcggga cctggatgcc gtagagcgcc tgtggctcga gtacctcacg tgggggaacg
     2221 acggactgca gtcgaggtac ggcttccgac tcccggtccg ggaggcggtg gagcatgacc
     2281 tcgcaaccat cgccaagttc cagccgcccg acggtcgcct cctcttggca ttcgagcgcg
     2341 acgtggccgt cggcacggcc tgcatgcggc ggatcggtcc cgacaccgcc gagatcaagc
     2401 gcatgtacat ccaaccctcg catcgccgtg ccggagtggg tcgcgcgttg caggaccggt
     2461 tgatcgcagc cgcgcggaac gccggatacg agcgcgtgag gctcgacagc cccgacttca
     2521 tgacggccgc gcacgacctg tatcgctcca gcgggttcgt tgagatcggc ccataccccg
     2581 agagcgagat ccccgacgag tacaagcccc actggctctt catggagcgg aggctcacct
     2641 gaaggctcgg gagcctcgtg caggtgttgg gcttggtagc gatacagtac tgcgccgtgc
     2701 aggcgggacc ggatgcgatc gatcgggtgg cgaagctcgc ggcgcgggtg accgacggcg
     2761 cggaggccgg gccggacgag ccgctcgccg cgctggggat cgactcgctg ggcatggccg
     2821 aactggctct ggccgtcgag gacgagttcg gcgtgcggct ggcggcggcc ccgccggagc
     2881 ggctgcgcac ggtgcgcgac gtggcccggg cggtggcgcg gctgcagggc cgggccgggg
     2941 gcccgcgcct cccggagggg atcgggcgcc tccaggacac cgcccggcga gcggttggac
     3001 cggtcctgac cgggtggtac cgcttccacg cggagggcgt cgagcagatc ccccggaccg
     3061 gcgccgccgt gatcgcggcg aaccacggca gcctgctcga cgtccccttc ctcgccctcg
     3121 cctgcccgcg cgacatctgg ttcatggcca agat
//
LOCUS       DASPWO010000015         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1020727, whole genome shotgun sequence.
ACCESSION   DASPWO010000015 DASPWO010000000
VERSION     DASPWO010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020727"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VEO00_00310"
     CDS             complement(<1..636)
                     /locus_tag="VEO00_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HYU56477.1"
                     /translation="MAEAGRNPLAGRSVLLGVSGGIAAYKAAVLARLLVRAGAAVQAV
                     LTPTATRFVGPATFAALTGRPARSDVMEEPERVVHVRMAREADLAIVAPATANVLARL
                     ALGLADDLLTSTLLEARCPLVLAPAMHSGMLEHPATRAHLDTLAARGAVVVGPVTGPL
                     AAGDEGPGRMSEPEEILAAAEAVLGGPARGDLASRTVLVTSGPTHEPIDPVR"
     gene            complement(674..922)
                     /gene="rpoZ"
                     /locus_tag="VEO00_00315"
     CDS             complement(674..922)
                     /gene="rpoZ"
                     /locus_tag="VEO00_00315"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HYU56478.1"
                     /translation="MIEPKIDDLLDRVDSKYSLVILAAKRAREINSYYNQLGEGRGEF
                     VPPLVESGGLRTKPLAIALQEIAEGKVSYERPETPEPA"
     gene            complement(978..1514)
                     /gene="gmk"
                     /locus_tag="VEO00_00320"
     CDS             complement(978..1514)
                     /gene="gmk"
                     /locus_tag="VEO00_00320"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HYU56479.1"
                     /translation="MVSGPSGVGKGTVGQALRERLPDLEYSVSLTTRPPRAVEEHGRD
                     YHFISPEDFQTLILEDAFLEWADVYGHRYGTLAGPVEEARAAGRDVLLELDVQGAAAI
                     RARVPDAVLIFLVPRSIEELEARLRRRGTEEAAALDERLSQARREMEDADRFDHVVVN
                     DDLETAVSEVAAIITGGD"
     gene            complement(1552..1866)
                     /gene="mihF"
                     /locus_tag="VEO00_00325"
     CDS             complement(1552..1866)
                     /gene="mihF"
                     /locus_tag="VEO00_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HYU56480.1"
                     /translation="MPLPTLTEEQRKQALEKAAEARKARAALKGKLKAEQMTLRDVLA
                     KPGDEIVGKMRVSAVLESLPGVGKVRARKIMERLEISPSRRVKGLGTKQREALLAEFG
                     KK"
     gene            complement(2033..>2216)
                     /locus_tag="VEO00_00330"
     CDS             complement(2033..>2216)
                     /locus_tag="VEO00_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HYU56481.1"
                     /translation="LARAAGAKPLGVAALVDRTNVDPGFELRALVRIDARTYDPNACP
                     LCRAGVPLTERGSRSL"
BASE COUNT          282 a          810 c          798 g          326 t
ORIGIN      
        1 gcgcaccggg tcgatcggct cgtgggtcgg gcccgacgtg acgagcacgg tgcggctggc
       61 caggtcgcca cgggccggcc ctcccagaac ggcctcggcg gcggccagga tctcctctgg
      121 ctccgacatc cgccccgggc cctcgtcgcc ggcggcgagc ggccccgtga ccgggccgac
      181 gacgaccgcc ccacgggcgg ccagggtgtc gaggtgggcg cgcgtggccg ggtgctcgag
      241 catcccggag tgcatcgccg gggcgagcac gagcgggcac cgcgcctcga gcagtgtgga
      301 ggtgagcagg tcgtccgcga gacccagggc gagccgtgcg agcacgttcg cggtggcggg
      361 agcgacgatg gccaggtcgg cctcgcgtgc catccggacg tggacgacgc gctcgggctc
      421 ctccatcacg tcgctgcgcg ccgggcggcc ggtcagcgcg gcaaaggtgg ccggcccgac
      481 gaagcgggtc gccgtggggg tcagcacggc ctggacggcg gccccggccc tgaccagcag
      541 ccgggccagc acggcggcct tgtaggcggc gatgccgccg ctcacgccga gcaggaccga
      601 ccgaccggcg agggggttcc gtccggcttc ggccatgccg tccctcctgc tggcgcggcc
      661 gtgcggttcg ggcctacgcc ggctcggggg tctccgggcg ctcgtaggag accttgccct
      721 cggcgatctc ctgcagggcg atcgccagcg gcttggtgcg caggccgccg ctctccacga
      781 gcgggggcac gaactcgccc cggccctcgc cgagctggtt gtagtaggag ttgatctccc
      841 gcgcccgctt ggcggcgagg atgaccaggc tgtacttgga atcgaccctg tccagcaggt
      901 cgtcgatctt cggctcgatc acgcgttctc ctgcggtcgg tggcggggcg gtcggaaggc
      961 ccgaggccca cgctccgcta gtccccgccc gtaattatag cggccacctc cgacacggcg
     1021 gtctccaggt cgtcgttcac cacgacgtgg tcgaagcggt cggcatcctc catctcccgg
     1081 cgggcctgcg acaggcgctc atcgagggcc gccgcctcct cggtcccccg ccggcgtagg
     1141 cgcgcctcga gctcctcgat cgagcggggg accaggaaga tcagcacggc gtcggggacc
     1201 cgggcccgga tggcagccgc cccctggacg tcgagctcga gcagcacgtc gcggcccgcg
     1261 gcccgggcct cctcgaccgg cccggcgagc gtcccgtagc gatggccgta cacgtcggcc
     1321 cactcgagga acgcgtcctc gaggatcagc gtttggaagt cctcggggct gatgaagtga
     1381 tagtcgcggc cgtgctcctc caccgctcgc ggcgggcgcg tggtgagcga aaccgagtac
     1441 tcgaggtcgg ggagccgctc acgcagcgcc tgcccgacgg ttcccttgcc gacgcccgag
     1501 gggccggaca ccacgaacag ccggccgcgg ggttcggcgt cgatggcggc gctacttctt
     1561 gccgaactcg gcgaggagcg cctcccgctg cttcgttccg agccccttca cccggcggct
     1621 gggggagatc tcgagacgct ccatgatctt gcgggcgcgc accttgccca cgccgggcag
     1681 cgactcgagc accgccgaga ccctcatctt gccgacgatc tcgtcgcccg gcttcgccag
     1741 gacgtcgcgc agggtcatct gctccgcctt gagctttccc ttcagcgcgg ctcgcgcctt
     1801 ccgggcttcc gccgccttct cgagtgcctg cttgcgctgc tcctcggtga gggtgggcaa
     1861 gggcatgagc atcttcccct ttcgattccg tgccgggatg cggcgcggat tctacccccg
     1921 ccccgagcac ggcgacaatc cgatgccgcg cgcgacctgc actttcgtgt cacggcctcg
     1981 acctccacgc gagggttaca ggagacgacc ttcgtcgccg tcgctcgact tgctagagcg
     2041 agcgagaacc tcgctcggtg agcggtacgc cggcccggca caacggacac gcgttggggt
     2101 cgtacgttcg ggcgtcgatg cgcaccaacg cgcgcaactc gaagccgggg tcgacgttcg
     2161 tccggtcgac gagcgcggcg acgccgagcg gtttagcgcc cgccgcgcgc gccaga
//
LOCUS       DASPWO010000016         6113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_935417, whole genome shotgun sequence.
ACCESSION   DASPWO010000016 DASPWO010000000
VERSION     DASPWO010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935417"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VEO00_00335"
     CDS             complement(<1..493)
                     /locus_tag="VEO00_00335"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HYU56482.1"
                     /translation="MEDRVRDRIADWVAALGRRQLIALAIGGVALLAGAGLWYVRSLP
                     RPVEIAAQPARASPTPSPALLVVHVAGWVVHPGVYELHQGERVIDAIELAGGARKGAD
                     LSALNLAALLQDAQQVLVPRAAPSGGAAGAAGAAPGAPGALMNLNVATAEQLETLPGI
                     GPVL"
     gene            complement(607..3042)
                     /gene="leuS"
                     /locus_tag="VEO00_00340"
     CDS             complement(607..3042)
                     /gene="leuS"
                     /locus_tag="VEO00_00340"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HYU56483.1"
                     /translation="MRPYDPAEIEPKWRARWEAEELYRAGGPDDPRPRFYALDMFPYP
                     SGDLHMGHAEAFAIGDAVARYAWMRGFDVLHPIGWDAFGLPAENAAIKRGIHPKEWTY
                     ANIEQQRDSFKAYGISFDWTRQFNTCDPEYYRWTQWLFLQLHARGLAYRKQAPANWCP
                     NDRTVLANEQVIGGRCERCGTVVVRKDLTQWFFRITDYAERLLDDMRDLEGAWPERVL
                     TMQRNWIGRSEGAEVDFEVAGETVTVFTTRPDTLWGVTFFVFAPEHPLVARLAHGSGV
                     ADEVAAMVEAARSTPLAEREEAAAVAASKEGIRLGVDAVNPVNGERAPCFVAPYVLME
                     YGTGAVMGVPAHDQRDFEFARAHGLPIRVVVEPPGERRDPETMTEAYDHEGGMVNSGP
                     LDGTPSPEGIATVIAWLEERGAGRKAVQYRLRDWLISRQRYWGCPIPIVHCPEHGEVP
                     IPDSDLPVLLPDDVDFAPHGDSPLAMHEGFVNTTCPIGGEPARRETDTMDTFVDSSWY
                     FLRYLSPHDAREAFDPDEVRRWMPVEQYTGGIEHAILHLLYARFFIKALQDAGYLGFT
                     EPFLRLQNQGQVIMGGEAMSKTTGNLVEFAAERAKYGADGVRLTVLSANAPGDDIDWA
                     DVNPSGVRAFLGRAWRAVHDAVESTAAEPEELRKLTHRTIKLVGEDLDAMQTHTAVSK
                     LMVLTREMRRALDAGAGAREAAEALARMLAPVAPFIAEELWRETLGHSDSVHRERWPE
                     HDEALARLERVTLVVQVDGRVRDRIEVEPDISEEEAVRLALASENARRFLDGGEPARI
                     VPRPPRLVNLVTR"
     gene            3251..4387
                     /locus_tag="VEO00_00345"
     CDS             3251..4387
                     /locus_tag="VEO00_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56484.1"
                     /translation="MTDTRPGPASATPAAAGAREPVSHARLVASKIAHFAAALFLFVL
                     AIQLMKKGAQALAPRLESSGFFDNGVSTLGLGWMGAYFVLSGSPIAATALTFRAANGI
                     TELQTFTMLSGSRLGASFIVLLVGFLYAVRSPNRRESLGMGVLALSMTALVYVPGMVL
                     GYGILRSGLLDGMHWTASAQLQGAIDAGWGWIVQLTTDHLPHALLFPIGLGVLLVSFK
                     LLDRVLPSLDGERHADSRGHWLKKPWPMFFLGCLAALLTLSVSVALTVLVPLASKGYV
                     RREEAIPYIAGANITTLADTLVAAMLLGSTSGVHVVLAEAIAVALITIVLLAFAYRHV
                     QRGVMALDEWVVGTSLRLWLFVGALFVVPVALMLAGQMIGPIGR"
     gene            4453..5598
                     /locus_tag="VEO00_00350"
     CDS             4453..5598
                     /locus_tag="VEO00_00350"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU56485.1"
                     /translation="MARIEVLRPVVDGRFRPGDAFRARMVQALLDSGFTAEQVEGAVA
                     GGMLDLDHVDRFPLVQPGRRSDRTFEEFLRRAGPRAARVLPAVYAVMGLPQPDPAAPI
                     REDEEALLAQFLEAWSLAGDEALIRAARLLGEGTRMAAFGWPDLFSEQVAAAARERWL
                     QGEVERYPPEVIRTGTLLASLLPRLMTWLNQRFLEQRIVAGIVDNFEDVLAARGLAPS
                     PEPSAPPAVVFADLSDYTRATEERGDETAVHLAATLQERAERVAAAREGRLVKLLGDG
                     AMLHFRDAARGVEGALELVAAVTEALGLPTHAGVHAGPVIERDRDLFGRTVNLASRIA
                     GRAGPGEVLVSELAARAAAGSGISLEDLGTVAVKGVAEPLRLYRATR"
     gene            5756..6112
                     /locus_tag="VEO00_00355"
     CDS             5756..6112
                     /locus_tag="VEO00_00355"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYU56486.1"
                     /translation="MRSIARDTSILVRKEVELARQELTEALKARLKAVAALAAAGVLA
                     LLALAFLGAAAAEALEGPLSPWGARLAVAGGFLIVALGSLLFARARMRRPSLAPEETV
                     RTVKEDVEWARAQLKR"
BASE COUNT          799 a         2227 c         2202 g          885 t
ORIGIN      
        1 ccagcaccgg gccgatgccc ggcagcgtct cgagctgctc ggccgtcgca acgttcaggt
       61 tcatcagggc gcccggcgcg ccgggagcgg cgccggccgc ccccgccgcc ccgccggacg
      121 gcgcggcccg cgggacgagc acctgctggg cgtcctgcag cagggcggcc agattcagcg
      181 ccgagaggtc ggcgcccttg cgcgcaccgc ccgcgagctc gatggcgtcg atcacccgct
      241 cgccctggtg cagctcgtag acgccggggt ggacgaccca gccggcgacg tgcaccacca
      301 gcagcgccgg ggagggcgtg ggggacgccc gggcgggctg cgcggcgatc tcgacgggcc
      361 gcggaagcga gcgcacgtac cacagtcccg cgccggccag gagggccacc ccgccgattg
      421 ccagcgcgat cagctgccgg cgcccgagcg ccgccaccca gtccgcgatc cgatcgcgga
      481 ctcgatcctc cacgccggaa cctccttggc tcgcgggggg aggaggggga cggcggcatg
      541 ctagcgcccg ccactgacac gtttccgcgg gcgggtcgcg ggcgagggcc gctgggcggc
      601 cggtcgctac cgggtgacca ggttgaccag gcgcgggggg cggggaacga tgcgcgccgg
      661 ctcgcccccg tccaggaagc ggcgggcgtt ctcggaggcc agggccaggc gcacggcctc
      721 ctcctcggag atgtccggtt ccacctcgat ccggtcccgg acccggccgt ccacctgaac
      781 gaccagcgtg acgcgctcca gccgcgcgag cgcctcgtcg tgctccggcc aacgctcgcg
      841 gtggacgctg tcggagtggc caagcgtctc gcgccacagt tcctcggcga tgaacggggc
      901 gaccggggcc agcatccgtg ccagcgcctc ggcggcctcc cgggcgcctg ccccggcgtc
      961 cagcgcgcgg cgcatctcgc gggtcaggac catcagcttc gagaccgcgg tgtgggtctg
     1021 catggcgtcc agatcctcgc cgaccagctt gatcgtgcgg tgggtcagct tccgcagctc
     1081 ctccggctcc gcggccgtgg actccacggc atcatggacc gcccgccacg cccgtcccag
     1141 gaacgcgcgc acgccggacg gattgacgtc ggcccagtcg atgtcgtcgc cgggagcgtt
     1201 cgccgacagc acggtcaggc gcacgccgtc cgccccgtac ttcgctcgct cggcggcgaa
     1261 ctcgaccagg ttgcccgtgg tcttgctcat cgcctcaccg cccatgatca cctgcccctg
     1321 gttctgcagg cgcaggaagg gctccgtgaa ccccaggtac ccggcgtcct gcagtgcctt
     1381 gatgaagaac cgcgcgtaca gcaggtgcag gatggcgtgc tcgatgccgc cggtgtactg
     1441 ctcgaccggc atccagcggc gcacctcgtc gggatcgaac gcctcccgcg cgtcgtgggg
     1501 cgacaggtag cgcaggaagt accaggacga gtcgacgaac gtgtccatcg tgtcggtctc
     1561 gcggcgggcg ggctccccgc cgatcgggca ggtcgtgttc acgaagccct cgtgcatcgc
     1621 gagcggcgag tcaccgtgcg gcgcgaagtc cacgtcgtcg gggagcagga ccggcaggtc
     1681 ggaatcaggg atggggacct cgccgtgctc gggacagtgc acgatcggga tcgggcagcc
     1741 ccagtagcgc tgtcgagaga tcagccagtc ccgcaggcgg tactggacgg ccttccgtcc
     1801 ggcgccgcgc tcctccagcc acgcgatcac cgtcgcgatg ccctcgggag aaggcgtccc
     1861 gtccagcggg ccggagttca ccatcccgcc ctcgtggtcg taggcctcgg tcatggtctc
     1921 ggggtcgcgg cgctctcccg ggggctcgac cacgacgcgg atcggaaggc cgtgggcccg
     1981 ggcgaactcg aagtcgcgct ggtcgtgcgc cggaacgccc atcaccgcgc cggtgccgta
     2041 ctccatcagc acgtagggcg cgacgaagca gggcgcgcgc tcaccgttca ccgggttgac
     2101 cgcgtcgacg cccagccgga tgccctcctt cgacgcggcg acggcggccg cctcctcgcg
     2161 ctcggccagc ggggtggagc gcgcggcctc gaccatggcg gccacctcgt ccgcgacgcc
     2221 ggagccatgc gccagccggg ccacgagggg gtgctccggc gcgaagacga agaacgtcac
     2281 gccccacagc gtgtccggac gggtggtgaa gaccgtcacg gtctcccccg ccacctcgaa
     2341 gtcgacctcg gcgccctcgg agcggccgat ccagttgcgc tgcatcgtca ggacgcgctc
     2401 gggccaggcg ccctccaggt cgcgcatgtc gtccagcagg cgctccgcgt agtcggtgat
     2461 ccggaagaac cactgcgtca ggtccttgcg caccaccacg gttccgcacc gctcgcaacg
     2521 gccaccgatc acctgctcgt tcgccaggac cgtccggtcg ttggggcacc agttggcggg
     2581 ggcctgcttg cggtaggcga ggccgcgcgc gtgcagctgc aggaacagcc actgcgtcca
     2641 tcggtagtac tccggatcgc acgtgttgaa ctgccgcgtc cagtcgaagc tgatgccata
     2701 cgccttgaac gagtcgcgct gctgctcgat gttggcgtac gtccattcct tcgggtggat
     2761 gccgcgcttg atcgcggcgt tctcggccgg caggccgaac gcgtcccacc cgatcgggtg
     2821 cagaacatcg aacccgcgca tccaggcgta ccgggcgacc gcgtcaccga tggcgaaggc
     2881 ctcggcgtgc cccatgtgca ggtcgccgga cgggtacggg aacatgtcca gcgcgtagaa
     2941 ccgcggccgg ggatcgtccg ggccgccggc ccggtacagc tcctcggcct cccaccgagc
     3001 ccgccacttg ggctcgatct cggctggatc gtagggtcgc accgccgcct tcctcttcga
     3061 attccgggaa cgaacgcgcc ccgggccagc gactggggca acccccgcgc tacgaacccg
     3121 gacggtggat tgctatccta ccgcgcgagg ttccggccga agggccgatc gccgccgaac
     3181 aggtacagag aggaactccc tgaacccccc cgagcattcc ggcacggccg tcgatgccga
     3241 ggccgacccg gtgaccgaca cgcgcccggg acccgcttcg gccacgccgg ccgcggcggg
     3301 cgcgcgcgag cccgtctccc acgcccggct ggtggcctcg aagatcgcgc acttcgccgc
     3361 ggccctgttc ctgttcgtcc tggcgatcca gctgatgaag aagggggcgc aggcgctcgc
     3421 gccgcgtctg gagagctccg ggttcttcga caacggcgtg tccacgctgg ggctcggctg
     3481 gatgggggcc tacttcgtgc tctccggctc gccgatcgcg gccaccgcgc tgacgttccg
     3541 ggcggcaaat ggcatcaccg agctgcagac cttcaccatg ctctccgggt cgcgcctcgg
     3601 agcgtccttc atcgtgctgc tggtcggatt cctctacgcg gtccgcagcc cgaaccgccg
     3661 ggagtcgctc gggatgggcg tgctggcact ctcgatgacg gcgctcgtct acgtgccggg
     3721 gatggtgctc ggctacggca tcctgcggtc ggggctcctg gacggcatgc actggaccgc
     3781 ctccgcgcag ctgcagggcg cgatcgacgc cggctggggg tggatcgtcc agctgaccac
     3841 cgaccatctc cctcacgccc tgctgttccc gatcggtctc ggcgtcctgc tggtctcgtt
     3901 caagctgctc gaccgggtcc tcccgtcgct cgacggcgag cgccacgccg actcccgcgg
     3961 gcactggctg aagaagccgt ggccgatgtt cttcctcggc tgcctggccg ccctgctgac
     4021 gctctcggtc tcggtcgcgc tgaccgtcct cgtcccgctg gcgagcaagg gctacgtccg
     4081 tcgcgaggag gccatcccct acatcgccgg cgcgaacatc accacgctgg cggacacgct
     4141 ggtcgccgcg atgctgctcg gcagcacgag cggcgtgcac gtggtgctgg ccgaggcgat
     4201 cgccgtggcc ctgatcacga tcgtcctgct ggccttcgcc taccgccacg tgcagcgggg
     4261 ggtgatggcc ctcgacgagt gggtggtcgg cacctcgctg cgcctgtggc tgttcgtcgg
     4321 ggcgctgttc gtggtcccgg tggccctgat gctggccggc cagatgatcg ggccgatcgg
     4381 ccggtaggat cgaccatcac cttcgaagac ctcgccgccg agaccggcat cccgctcgac
     4441 cgtctggatt ggatggcccg gatcgaggtc ctgaggccgg tcgtggacgg ccgcttccgc
     4501 cccggggacg ccttccgcgc gcggatggtg caggccctgc tcgacagcgg cttcaccgcc
     4561 gagcaggtgg agggcgccgt ggcgggcggc atgctcgacc tcgatcacgt ggatcggttc
     4621 ccgctggtcc agcccggccg gcggtctgac cggacgttcg aagagttcct gcggcgggcc
     4681 gggccgcgcg cggcacgcgt gctgcccgcc gtctacgcgg tgatggggtt gccgcagcct
     4741 gaccccgccg cgccgatccg ggaggacgag gaggcgctcc tggcgcagtt cctggaggcc
     4801 tggtcgctcg ccggcgacga ggcgctgatc cgggcggccc gcctgctggg cgagggcacc
     4861 cggatggccg cgttcggctg gccggacctc ttctcggagc aggtggccgc ggcggcccga
     4921 gaacgctggc tgcagggcga ggtcgagcgg tacccgccgg aggtgatccg taccggcacg
     4981 ctgctggcct cgctccttcc gcggctgatg acctggctga accagcgctt cctggagcag
     5041 cggatcgtcg ccggcatcgt cgacaacttc gaggacgtgc tcgccgcccg tgggctggcg
     5101 ccgtcgccgg agcccagcgc cccgccggcc gtggtcttcg ccgacctctc cgactacaca
     5161 cgggcgaccg aggaacgggg cgacgagacg gcggtgcacc tggccgccac gctgcaggag
     5221 cgtgccgagc gcgtggcggc cgcccgcgag ggacgcctgg tgaagctgct cggcgacggc
     5281 gcgatgctcc atttccgcga cgccgcccgg ggggtggagg gcgccctcga gctggtggcg
     5341 gcggtgaccg aggccctcgg cctcccgacg cacgccgggg tgcacgccgg gccggtgatc
     5401 gagcgcgacc gcgacctgtt cgggcgaacg gtgaacctgg cctcgcggat cgccgggcgc
     5461 gccgggccgg gcgaggtcct ggtcagcgag ctcgccgccc gcgcggccgc gggctccggc
     5521 atctccctgg aggacctcgg cacggtcgcg gtgaagggcg tcgccgagcc cctccgcctc
     5581 taccgcgcca cgcggtagag gcagggttat gcacgccggt acagggaatg cctgaggcgt
     5641 gcgccccgcg tcgcgcctga ggaaccggat ggacaccctg gagcgaacga atcgtgtctc
     5701 ggcgactctc gccggcgccg agcctggacc gcgggaccgt tcgaccgccg atctgttgag
     5761 gtcgatcgcc cgtgacacct ccatcctcgt tcgcaaggag gtcgagctcg cccggcagga
     5821 gctgaccgag gcgctcaaag cgcggctgaa ggccgtcgcc gccctcgccg cggcaggggt
     5881 gctggcactc ctcgcgctgg cattcctcgg agccgcggcc gcggaggcgc tcgagggccc
     5941 cctctccccg tggggggcgc gactggcggt ggcgggtggg ttcctgatcg tggcgctcgg
     6001 cagcctcctg ttcgcccgcg ccaggatgcg acgaccttcg ctggcacccg aggagacagt
     6061 ccgcaccgtg aaggaggacg tcgagtgggc gagagcgcag ctgaaacggt aag
//
LOCUS       DASPWO010000017         4277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_341487, whole genome shotgun sequence.
ACCESSION   DASPWO010000017 DASPWO010000000
VERSION     DASPWO010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341487"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..941)
                     /locus_tag="VEO00_00360"
     CDS             complement(192..941)
                     /locus_tag="VEO00_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYU56487.1"
                     /translation="MSGHSKWSQIKRKKGVADQQRGKLFAKLLRYVEVAAREGGGSVE
                     GNATLAAAVQKARDASVPVDTIERAIKRGTGELEGARYEEATYEGFAPGGVALLIEVL
                     TDNRNRSSADIRHAMTRFGGSLGDPGSVAWMFERRGVVIVDRAAAPDEERLLEVVSEA
                     GAEDLNDLGDQWEVVTPPQSLRAVREALEAAGIAVASADLTMQPQTLVPVARDQAAGV
                     LRLLEALEDLEDVQAVHANFDIPEEVMAEVG"
     gene            complement(945..1502)
                     /gene="pdxT"
                     /locus_tag="VEO00_00365"
     CDS             complement(945..1502)
                     /gene="pdxT"
                     /locus_tag="VEO00_00365"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HYU56488.1"
                     /translation="MKAGVLALQGDFREHGRVLADLGATPVEVRTPEELAGVACLAIP
                     GGESTTISLLARARGLVEPIRERAAAGMPVLGTCAGLIVMAARVEGGDPLLSLLDATV
                     RRNAYGRQVESFEADVDLAGEPEPVRGVFIRAPVIEAVGDGVSVIATLDGAPVAVEQD
                     NLIGVAFHPELAGDQRLHRRLLERA"
     gene            complement(1585..2472)
                     /gene="pdxS"
                     /locus_tag="VEO00_00370"
     CDS             complement(1585..2472)
                     /gene="pdxS"
                     /locus_tag="VEO00_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HYU56489.1"
                     /translation="MAESTGTFRVKSGLAEMLKGGVIMDVTTADQARIAEEAGACAVM
                     ALERVPADIRKEGGVARMADPRRVEEIMAAVSIPVMAKCRIGHFVEAQILQSLGVDYV
                     DESEVLTPADEGHHINKWVFAVPFVCGARDLGEALRRLAEGAAMIRTKGEAGTGNVVE
                     AVRHVRAITGELSRLHGMRDDELVSAAKDLGAPLELVRQVAATGTLPVVNFAAGGLAT
                     PADAALMMQLGVDGVFVGSGIFKSEDPAMRARAIVEATTSFNDPDVLAKVSRGLGEPM
                     RGLDIAEIGEEHLLQTRGW"
     gene            complement(2545..3585)
                     /locus_tag="VEO00_00375"
     CDS             complement(2545..3585)
                     /locus_tag="VEO00_00375"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HYU56490.1"
                     /translation="MAFTTRGKVVTVLLTLVLVAGGVYVWLVLTKKIEGPISLLNPPD
                     SSADVPDNCPLTGVNPDGDVPDRPALAIKVENADGARPQAGMERADVTYEERVEGGIT
                     RFIVIYQCQDSERIGPVRSARPVDPDVLLQLGTETVFGYSGAADEVLKIVARSGLVDV
                     NFGKFVEAYRRDPNREQPHNLYTTTEDLYAAAKDPTGVPKTLWQYDPAEPEQAARPAS
                     SIHVPFLEADVVWTWDAKRGLWFRAQDGGPHLMEDGTQLLATNVIVQEVQTFCSGLYD
                     VNNVCVDEVSVVGTGNAIVFRNGKAIRGTWVREFTEDVTKFLDRRGREIRLDPGTTWV
                     ELVPTDIKATFQ"
     gene            complement(3719..>4277)
                     /locus_tag="VEO00_00380"
     CDS             complement(3719..>4277)
                     /locus_tag="VEO00_00380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYU56491.1"
                     /translation="PEHSSVAVTQGLLDMMNRLELEGVLAHELSHVKNRDILVGTIAA
                     TLAGAVVLLADIMLRTLFWGGGRRGRDRDGGGAFAVVALLGIALAILAPLFAQIIRLA
                     VSRRREYLADADGAMLTRYPPGLASALRKIAADHTPLRVANNATAHLWLAPATRTPGP
                     KLTLWERLFDTHPPIEERIRILEEM"
BASE COUNT          588 a         1596 c         1455 g          638 t
ORIGIN      
        1 ccttgcggta gtgctcggca acctcgcgca ggaacgcctg gacctctccc ctgtcgtagc
       61 cgcgcacgga gacgaggaac tccttgcctt cgatgtcctc ggggagcacg ggcctcacca
      121 tccttcggat cgggacagga ccccggagta tcccacgggc gtccagccgg tacccctccc
      181 ccgggaggcc atcagccgac ctcggccatg acctcctcgg ggatatcgaa gttcgcgtgc
      241 acggcctgga cgtcctcgag gtcctcgagc gcctccagca ggcgcagcac gccggcggcc
      301 tggtctcggg ccacggggac cagggtctgc ggctgcatcg tcaggtcggc ggaggccacg
      361 gcgatcccgg ccgcctccag cgcctcgcgc accgcgcgca gtgactgcgg cggcgtgacg
      421 acctcccact ggtcgccgag gtcgttcagg tcctcggcgc ctgcctcgct gaccacctcc
      481 agcagccgct cctcgtcggg cgcggcggct cggtcgacga tcaccacgcc gcgccgctcg
      541 aacatccagg ccacgctccc ggggtcgccc aggctcccac cgaacctcgt catcgcgtgc
      601 cggatgtcgg cggaggagcg gttgcggttg tcggtcagca cctcgatcag cagcgcgacc
      661 ccgccggggg cgaagccctc gtaggtggcc tcctcgtagc gcgcgccttc gagctcgccg
      721 gtgccgcgct tgatggcccg ctcgatggtg tccaccggga ccgaggcgtc ccgcgccttc
      781 tgcaccgcgg cggcgagcgt ggcgttcccc tcgacgctcc cgcccccttc gcgggcggcc
      841 acctccacgt accgcaggag cttggcgaag agcttgccgc gctgctggtc ggcgacgccc
      901 ttcttgcgct tgatctggct ccacttcgag tgcccggaca tcgctcaggc cctctcgagg
      961 agccggcgat ggagccgctg gtcgccggcg agctccgggt ggaaggccac cccgatcagg
     1021 ttgtcctgtt caaccgccac cggcgctccg tccagcgtcg cgatcaccga gacgccgtcc
     1081 ccgaccgcct cgatcaccgg agcccggatg aacaccccgc gcaccggctc gggctcgccg
     1141 gccaggtcga cgtccgcctc gaacgactcc acctggcgcc cgtaggcgtt gcgccgcacg
     1201 gtggcgtcga ggagtgacag gagcggatcg cccccctcga cccgcgccgc catcacgatc
     1261 aggccggcgc aggtcccgag gaccggcatg ccggccgcgg cccgctcgcg gatcggctcg
     1321 accaggcctc gcgcgcgcgc caggagcgag atcgtcgtgg actcgccgcc ggggatggcc
     1381 aggcaggcca ctccggccag ctcctccggc gtgcggacct cgaccggcgt cgcgcccaga
     1441 tcggccagca cccgcccgtg ctcccggaag tcgccctgca gggccagcac tcccgccttc
     1501 atgtcaccct gggggttcgc tcgccgaacg tggtcgggcc cctcgcacgt cccagcgcga
     1561 gctccccccc gaacccccca cgtcctacca accccgggtt tgcaggaggt gctcctcgcc
     1621 gatctcggcg atgtccaggc cgcgcatcgg ctcgccgagg ccgcgagaca ccttggccag
     1681 cacgtccggg tcgttgaagg acgtggtcgc ctcgacgatc gcccgggcgc gcatggccgg
     1741 gtcctcggac ttgaagatgc cggagccgac gaacactccg tccacgccga gctgcatcat
     1801 cagcgcggcg tcggccgggg tggccaggcc gcccgccgcg aagttcacca cggggagcgt
     1861 cccggtggcg gcgacctgcc ggacgagctc gagcggcgcg ccgaggtcct tcgccgccga
     1921 gaccagctcg tcgtcgcgca tcccgtgcag gcgggaaagc tcgccggtga tcgcccggac
     1981 gtggcggacg gcctcgacca cgttccccgt cccggcctcg cccttcgtcc ggatcatcgc
     2041 ggcgccctcg gcgagccggc ggagcgcctc gccgaggtcg cgggcgccgc acacgaacgg
     2101 gaccgcgaag acccacttgt tgatgtggtg gccctcgtcg gccggcgtca ggacctcgct
     2161 ctcatccacg tagtcgacac cgagggactg caggatctgc gcttcgacga agtggccgat
     2221 gcggcacttg gccatcaccg ggatcgagac ggcggccatg atctcctcga ccctcctcgg
     2281 gtcggccatc cgcgccacgc cgccctcctt gcggatgtcg gcggggaccc gctcgagggc
     2341 catcacggcg caggccccgg cctcctcggc gatcctggcc tggtcggcgg tggtgacgtc
     2401 catgatcacc ccgcccttca gcatctcggc caggccggac ttcacccgga acgtgcccgt
     2461 gctctctgcc atcgcccctc ctcgaggaac cgatgaggaa ccgctctcct caaggatacg
     2521 cgcgccgggg gtgcgccagc ggggttactg gaaggtggcc ttgatgtcgg ttgggaccag
     2581 ctcgacccag gtggtgccgg ggtcgagccg gatctcccgg ccccggcggt cgaggaactt
     2641 cgtgacgtcc tcggtgaact cccggaccca ggtgccgcgg atcgcctttc cgttgcggaa
     2701 cacgatcgcg ttgccggtcc cgaccaccga gacctcgtcc acgcagacgt tgttcacgtc
     2761 gtagaggccg ctgcagaagg tctgcacctc ctggacgatc acgttcgtcg ccaacagctg
     2821 cgtgccgtcc tccatcaggt gcggcccgcc gtcctgggcg cggaaccaca ggccgcgctt
     2881 ggcgtcccag gtccagacca cgtcggcctc caggaacggg acgtggatcg acgacgccgg
     2941 ccgcgccgcc tgctcgggct cggccgggtc gtactgccag agtgtcttcg gcacgccggt
     3001 cgggtccttc gccgccgcgt agaggtcctc ggtggtggtg tagaggttgt gcggctgctc
     3061 gcggttcggg tcgcggcggt aggcctcgac gaacttgccg aagttcacgt ccacaagccc
     3121 ggaccgcgcg acgatcttca gcacctcgtc ggccgcgccg gagtacccga agacggtctc
     3181 ggtgccgagc tgcagcagga cgtcggggtc caccggccgc gcgcttcgca ccgggccgat
     3241 gcgttcggag tcctggcact gatagatgac gatgaaccgg gtgatcccac cctcgacgcg
     3301 ctcctcgtag gtcacgtctg ccctctccat gcccgcctgc ggccgcgccc cgtcggcgtt
     3361 ctccaccttg atcgccagcg caggccggtc cgggacgtcc ccatcggggt tcacgccggt
     3421 gagcggacag ttgtcgggga catcggccga ggagtcgggg gggttgagga ggctgatggg
     3481 cccctcgatc ttcttcgtca gcaccagcca cacgtagacg ccgccggcca cgagcaccag
     3541 cgtcagcagc acggtaacga ctttcccgcg cgtggtgaat gccacggtga atgaacctcc
     3601 ttggagtccg gggggagtcg aagaaacgtc gcccgatgct acgacgggac ctgggcgttc
     3661 acaaccaggg tggggtgttc ggggggggga acacgggggg gctgagcccc cccgtgcatc
     3721 acatctcctc caggatgcgg atgcgctcct cgatcggagg atgggtgtcg aacaggcgct
     3781 cccacagggt gagcttcggc cccggcgtcc gcgttgccgg cgcgagccag aggtgggcgg
     3841 tggcgttgtt cgccactcgc agcggggtgt ggtcggcggc gatcttgcgc agcgccgagg
     3901 ccaaccccgg cgggtagcgc gtcagcatcg cgccatctgc gtcggcgagg tactcgcgcc
     3961 ggcgcgacac cgccaggcgg atgatctgcg cgaacagcgg cgcgaggatc gccagcgcga
     4021 tgccgaggag cgccaccacc gcgaacgcgc cgcccccgtc ccgatcgcga ccgcgccgcc
     4081 cgccgcccca gaacagcgtc cgcagcatga tgtcggcgag cagcaccacc gccccggcga
     4141 gggtcgccgc gatcgtccca accaggatgt cgcggttctt gacgtgggac agctcgtgcg
     4201 ccagaacgcc ttcgagctcc agccggttca tcatgtcgag cagtccctgc gtcacggcga
     4261 ccgacgagtg ctcgggg
//
LOCUS       DASPWO010000018         7133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_256624, whole genome shotgun sequence.
ACCESSION   DASPWO010000018 DASPWO010000000
VERSION     DASPWO010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256624"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VEO00_00385"
     CDS             <1..378
                     /locus_tag="VEO00_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HYU56492.1"
                     /translation="AAAGVERVPRTAGGNGLPELEDGRVLEVANVIWCTGFRSDFGWI
                     ELPILGEDGHPVHDRGVVGSEPGLYFVGLPFLYSIPSVLIGGVGRDAEYIAAYIASRA
                     AGDRSSSDGAATSTHLRAERPNR"
     gene            534..1031
                     /locus_tag="VEO00_00390"
     CDS             534..1031
                     /locus_tag="VEO00_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYU56493.1"
                     /translation="MGDTLEVAIGTGLNLPHYPRNVRLTGVDLSPDMLARAVTRAREL
                     GRTIRFTEGDAERLPFADRSFDTVVCTYALCSVRDDAGAISEMHRVLRPGGRLILVDH
                     IRSSVPPIFWFQWLHEFIPRRTKGEYSTRRPSVHVMAGDFRIHARDRLRAGIIERLVA
                     VKTST"
     gene            complement(1509..1808)
                     /locus_tag="VEO00_00395"
     CDS             complement(1509..1808)
                     /locus_tag="VEO00_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HYU56494.1"
                     /translation="MAVMVTVRGRLRDTPEAIRKIHDEVTAATKDMARQAGDISHRVY
                     LNPQDPRDFLGIDEWETAEAVQAFSANPQIQQFFGQMFEALPETTIWEDSGWNQW"
     gene            complement(1825..1980)
                     /locus_tag="VEO00_00400"
     CDS             complement(1825..1980)
                     /locus_tag="VEO00_00400"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HYU56495.1"
                     /translation="MGEGEWHVRCDCGWEARAPKGKLVGLIQQHARDVHDMEITVDQA
                     LAQARPA"
     gene            2034..2804
                     /locus_tag="VEO00_00405"
     CDS             2034..2804
                     /locus_tag="VEO00_00405"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HYU56496.1"
                     /translation="MRCWVELRIFLAGRLAIASGTRLVDEHRFPGLQGRVVFAMLAAE
                     RERPVSREELADELWGDGLPPAWETSIRAIASKLRGMLGEAGVDGGRVLTGAAGLYQL
                     RLPGTWVDIDAAADAIHRAEPLLRRGRVDEACGWALAARAIARRPLLPGAEGPWVTHR
                     RDRLRDLHLRALEALAEIWLRRGVAALAVADAEEALRLEPFRESAHRALMRAHAAAGN
                     RAEALRAYEECRRLLGTDLGVDPSPETEGVYLEVLRSA"
     gene            complement(2992..3216)
                     /locus_tag="VEO00_00410"
     CDS             complement(2992..3216)
                     /locus_tag="VEO00_00410"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56497.1"
                     /translation="MRGRTLGSDEDNVSGVTTFEWLPGGFFLQQRVELVFAGYEINGL
                     EVIGYDPSIGTTQAWWGSLFPITTTCRGTA"
     gene            complement(3453..4463)
                     /locus_tag="VEO00_00415"
     CDS             complement(3453..4463)
                     /locus_tag="VEO00_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HYU56498.1"
                     /translation="MKAWQVREPGPIDSGPLELVDRPVPEPGPREVRLRVSTCGVCRT
                     DLHVAEGDLPVHRGHVVPGHEVVGRVEALGEGSSRFALGDRVGIAWLRFTCGRCRFCR
                     AGRENLCIAPRFTGYDEDGGYAEHAVVQEGYAYRLPDAFDDEHAAPLLCSGIIGYRAL
                     RRANPPPGGRLGVYGFGGSAHLATQVAVAEGHRVHVLTRSRRARELALSLGAASAGDA
                     ADPPPEPLDAAILFAPAGDLVPVALAALDRGGRLAIAGIHLTDVPSLRYADHLFQERE
                     IVTVTANTRRDGEEFLDIAARILVQVSTTSFPLEEAGVALRALAHGELTGAAVLNVAA
                     AT"
     gene            complement(4525..5550)
                     /locus_tag="VEO00_00420"
     CDS             complement(4525..5550)
                     /locus_tag="VEO00_00420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYU56499.1"
                     /translation="MPVRAVGYLGIATNRAHEARLRKDLRAAETMGLPGWHWLDAKAA
                     RERIDSPRVTAAVLEEACAILNPAALAHGLRRVALERGVRLYERTPVTGSEARGDGVR
                     FRTPFGDVRAARGVLAVNPWVKDFAPFRRLQVPVYTYAMASEPLTDEQISRIGWASRE
                     GVEDKRNYIHYFRLTKENRIVWAGSDAIYYYGSRIGPDLDRSAATFARLERSFRWYFP
                     HLAEIRFPYRWGGPVAITADFLPYIGTVRGRPLHYAFECNGHGVAPAHTWGQILRDLC
                     FERETDLTDLLFVGRRQPAFRPEPLRYLGAEATRRALLRQDRAMEAGKDAGDMDPWIL
                     RVLNKLG"
     gene            complement(6113..>7133)
                     /locus_tag="VEO00_00425"
     CDS             complement(6113..>7133)
                     /locus_tag="VEO00_00425"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYU56500.1"
                     /translation="VCFGNFVGGPSAGSNAVRLTLARSTDGGDTWSNVIVEPNTNSAS
                     GNWALAAGATGCTIRTDSAGNVYLFWHGFNQQTKAEAIFLSRSFDGGATFEMRRKLFI
                     VHPTGVFDPVIGRNTMDGIAGARSDLSTSPSVDIANGSPSGTGATDQIVLNWIDGQTL
                     NDEHVMFSTSTDGGDTWAAPVSIETNAGDRGYYTATAISPDGEDAWIAYNAFTAPYQT
                     TTANPRPLLAVVAHADVVGGAVGAFSEQHRSTPGDARSSSQNDLTAEFLGDYVYASAR
                     NGYAAFVWNDVRTGADCPAIDAWRAALRTKSTKDDPPRPEPNNDCAANFGDSSIFGAA
                     ILTPP"
BASE COUNT         1019 a         2495 c         2523 g         1096 t
ORIGIN      
        1 gccgccgcag gcgtcgagcg cgtgcccagg acggcaggcg ggaacggcct tcccgagctc
       61 gaagacggac gagtccttga agttgcgaac gtgatctggt gcacagggtt caggtcggac
      121 ttcggctgga tcgagcttcc gatcctgggg gaagatggtc acccggtgca cgaccgcggc
      181 gtcgtgggat cggagccggg cctgtacttc gtgggactcc cctttctgta ttcgatcccc
      241 tcggtgctga tcggtggggt gggcagggac gctgagtaca tcgccgcgta catcgcgtcc
      301 cgggctgccg gggatcgatc ctcatcggat ggggcagcga cctcgacaca tttgcgagcg
      361 gagcgcccga accgatgacc aggggtcccg agactctggg ggagagagct cgggcgatcg
      421 aagtcaagcg gcgcctctac gggaggaagg ctccgacgta tgaccgggag gcggacttca
      481 ccgaacgatg gctcttcggc accggacacc gaggctgggc gtgttcgaag gcgatgggtg
      541 acaccctcga ggtcgccatc ggcaccggtc tcaacctgcc ccactatccc cggaacgtgc
      601 gcctcaccgg agtcgacctc agtccggaca tgctcgcccg ggccgtgacg cgagccaggg
      661 agctggggcg cacgatccgg ttcaccgagg gcgacgctga gcgcctcccc ttcgcagacc
      721 gatcattcga caccgtcgtg tgcacgtacg ccctgtgcag cgttcgggat gatgccggcg
      781 ccatctcgga gatgcaccgc gtcctcaggc cgggcgggcg cctgatcctc gtggatcaca
      841 tccgcagctc cgtcccgccg atcttctggt tccagtggct gcatgagttc atccctcgtc
      901 gcaccaaggg tgagtactcg actcgacgac cgtcggtcca cgtgatggct ggtgacttcc
      961 gcatacacgc gcgtgatcgt ctgcgagcgg gcatcatcga gcgtctggtc gcggtgaaga
     1021 catccactta gggtgcggag gcggatggga atcgacccgc cagtgcgttt gcacgcgctt
     1081 cctggctttg aagaccgcgg tccgtctctg gtgaaggctg cgggctgatc ttggcgcgca
     1141 gttgggacct ggattcgtgc taacgcggtg ctaacgcacg tggggacaca ggctggtacc
     1201 aaatgacatg accggctcgg cgacccgcgc caaaatccct ggctagggga actgaggtgc
     1261 accagatgcc accagatgcc acgaccacgg tccgagttcg aatcccaccc cgggcacgtg
     1321 ctccgctgaa gaatcgcaaa cggtaggcgg gtctgctgtt cgcccgaaga ctgggcggcg
     1381 gcaagcgcca ggaaggccgg ctcgccgccg gcgaactcga acatcgaggg ccgagccacg
     1441 atccggagtc tcgcccgcag acctcggcgc gtccagcgtg gttcgaaggg ctcaggctct
     1501 cctgacgcct accactggtt ccagccgctg tcctcccaga tcgtggtctc aggcagcgcc
     1561 tcgaacatct gcccgaagaa ctgctggatc tgggggttgg ccgagaaggc ctgcaccgcc
     1621 tccgccgtct cccactcgtc gatcccgagg aagtcccgcg ggtcctgggg gttcaggtac
     1681 acgcgatgcg agatgtcccc cgcttgccga gccatgtcct tcgtcgccgc ggtgacctcg
     1741 tcgtgaatct tcctgatcgc ctccggggtg tctcgcagcc gcccgcggac cgtgaccatg
     1801 acagccatgc gtgcctcctt accctcaggc cggccgggct tgcgccagcg cctgatccac
     1861 ggtgatctcc atgtcgtgaa cgtcgcgagc gtgctgctgg atcagcccca cgagctttcc
     1921 cttcggcgct cgggcttccc agccgcagtc gcagcggaca tgccattcgc cctctcccac
     1981 ggggcctcac ctccgcggtg tgatactagg caagcgccgt tgggcgaacg ttggtgaggt
     2041 gttgggtgga gcttcggatc ttcctcgccg ggcgcctggc gatcgcctcg ggcaccaggc
     2101 tggtggacga gcaccggttc ccggggctcc aggggcgcgt ggtcttcgcc atgctcgccg
     2161 ccgagcgtga gcgcccggtc tcccgtgagg agctggccga cgagctctgg ggagacggcc
     2221 tgccgccggc ctgggagacg agcatccggg ccatcgccag caagctgcgc ggcatgctcg
     2281 gggaggcggg agtcgatggc gggcgggtcc tgacgggggc cgcgggcctg taccagctcc
     2341 ggctgcccgg gacgtgggtc gacatcgacg ccgcggccga tgccatccat cgcgccgagc
     2401 cgctcctgcg gcgcggacgg gtcgacgaag cctgcggctg ggccctggcc gctcgggcca
     2461 tcgcccggcg tcccctcctg cccggagccg aggggccgtg ggtcacccac cggcgggatc
     2521 ggctgcgcga cctccacctc cgggcgctgg aggctctggc cgagatctgg ctgcggcggg
     2581 gcgtcgcggc cctcgcggta gcggacgcgg aggaggccct ccggttggag ccgttccgcg
     2641 agtcggccca ccgggccctg atgcgggccc acgccgcagc cggaaaccgg gcggaggcgc
     2701 tgcgagccta cgaggagtgc cgcaggctgc tgggcacgga tctcggagtg gacccctcgc
     2761 cggagaccga aggggtctac ctggaggtcc tgcgctcggc ctgagtgaag tggatccgat
     2821 tggaacggcc ctcgatcgcg cgcgtgaccg tcgtacgatg accccggttc agctcgcgct
     2881 cccgggcgcg ccagtcgaag gactcggccc aatcgtcggg cctccagccc cccgagtacg
     2941 agttgacgcg gcagtgaaac gtcgcccctc cgccgaattc cgtccggatt gtcacgccgt
     3001 tcccctgcac gtcgtagtga tagggaagag ggatccccac catgcttgag tagtcccgat
     3061 ggacgggtcg tagccgatga cctccagacc attgatctcg tagccggcga agacgagttc
     3121 gacgcgctgt tgcaggaaga accccccggg cagccactcg aacgtcgtca ctcctgacac
     3181 gttgtcctcg tccgagccga gcgttcggcc ccgcatggtc cacctgccaa cgaaggattt
     3241 cagccgcttg agagccgggt cgggctctgg cggcccctgc cgtggcgcgg cgtcatctgt
     3301 catgcagtcc ctcctctcgg aagacgcgaa gacgtcgatc ccctgttccg cttgaacgtg
     3361 ccgcgtaccc ctcagcgagt tccgttcgtt gcggggcgac cggcgggagc tgccgacgtc
     3421 gtggtgatgg ttgcccgggg atcccgcgag ggtcacgtgg ccgcggcgac gttcagcacc
     3481 gcggcgccgg tcagctcccc gtgcgcgagc gcgcggagcg cgacgccggc ctcctcgagg
     3541 gggaacgacg tggtcgacac ctgcaccagg atccgggccg cgatgtcgag gaactcctca
     3601 ccgtcccgcc gcgtgttcgc ggtcacggtg acgatctccc gctcctggaa caggtgatcg
     3661 gcgtagcgca acgacggcac gtcggtgagg tggatccccg cgatcgccag acgcccgccc
     3721 cggtccagcg ctgccagggc cactggcacc agatcgcccg ccggcgcgaa caggatggcc
     3781 gcgtcgagcg gttccggcgg cgggtcggcc gcgtcgcccg cggaggcggc gccgagcgac
     3841 agggccagct ctcgagcccg gcgggaccgg gtcaggacgt ggacgcgatg gccctcggcc
     3901 accgcgacct gcgtggccag gtgcgccgag ccgccgaatc cgtagacgcc gagccgcccg
     3961 ccgggaggcg ggttcgcgcg ccgcagcgcc cggtacccga tgatccccga gcacagcagc
     4021 ggggcggcgt gctcgtcgtc gaacgcgtcg ggaaggcggt aggcgtagcc ctcctggacc
     4081 accgcgtgct ccgcgtaccc gccgtcctcg tcgtagcccg tgaaccgtgg cgcgatgcac
     4141 aggttctccc gcccggcgcg gcagaaccgg caccggccgc acgtgaagcg gagccaggcg
     4201 atcccgaccc ggtcgccgag ggcgaaccgc gacgatccct cgccgagcgc ctccacgcgc
     4261 ccgaccacct cgtggccggg gacgacgtgt ccccggtgga ccgggaggtc gccctcggcc
     4321 acgtgcaggt cggtccggca cacgccgcac gtgcttacgc ggagacgaac ctcgcgcggc
     4381 ccgggctcgg ggaccggccg gtcgaccagc tcgagcggtc cggagtcgat cggccccggt
     4441 tcccgaacct gccacgcctt cacgccacgg attctcccgc cgatgcgcgt ggcgcggacc
     4501 ggcctcgccc ggcggcgggc gccgctagcc gagcttgttc aggacccgca ggatccaggg
     4561 atccatgtcg ccggcgtcct tgcccgcctc catcgcccgg tcctggcgga gcagcgccct
     4621 ccgcgtggcc tcggcaccca ggtagcgcag tggctcggga cggaacgcgg gctgcctgcg
     4681 cccgacgaac agcaggtcgg tcaggtcggt ctcccgctcg aagcacaggt cgcgcaggat
     4741 ctggccccag gtgtgcgccg gcgccacacc gtggccgttg cactcgaacg cgtagtgcag
     4801 cggcctgcct cggacggtcc cgatgtaggg aaggaagtcg gccgtgatcg cgaccggccc
     4861 gccccagcgg tacgggaacc ggatctcggc gaggtggggg aagtaccagc ggaagctccg
     4921 ctcgagacgc gcgaacgtgg ctgcgctccg gtcgaggtcc gggccgatcc gcgagccgta
     4981 gtagtagatc gcgtcgctcc ccgcccacac gatgcgattc tccttggtca gccggaagta
     5041 gtggatgtag ttccgcttgt cctccacgcc ctcccgcgag gcccagccga tcctcgagat
     5101 ctgctcgtcc gtgaggggct cggaggccat ggcgtacgtg tagaccggga cctggaggcg
     5161 gcggaacggc gcgaagtcct tcacccaggg attgacggcg agcactcccc gagcggcccg
     5221 cacgtcgccg aacggcgtgc ggaagcggac gccgtccccc cgcgcctcgg agccggtgac
     5281 gggggtgcgc tcgtagaggc gaaccccgcg ttccagggcg acccggcgca gcccgtgggc
     5341 gagcgcggcc ggattgagaa tcgcacaggc ctcctcgagg accgccgcgg tgacccgcgg
     5401 ggagtcgatg cgctcccgcg ccgccttcgc gtcgagccag tgccagccgg ggaggcccat
     5461 cgtctcggcc gcccggaggt ccttccgcag tcgcgcctcg tgggcccggt tcgtggcgat
     5521 cccgaggtac ccgaccgctc gtactggcag tcgatcccgt tcttggtggc gaactcgccg
     5581 atctcgtcga ccgagcgggc caccgcgagg tgcgcggccc tggcccgggc cgcgcctggt
     5641 ggaccagcag gtggtggagg ctcatgtcga gcagggtcat cgcgaacccg ccgttgcgcc
     5701 cggagcctcc gaagccggtc acttccttct cgaggagcac gacgtcgagc gcgggatcgg
     5761 cctccttcag gaacaacgcc gtccacagac ccgtgtagcc gcccccggcg atgacaacgt
     5821 cggcctccgt ggacgcttcc agcggcgcca ccggcgcgta gtcgtaggtg gcgagccaga
     5881 aggacctcga cttccagctc ccctcgtcca gccccggcgc catggcgagc ctccaagatc
     5941 ggtgaaccgc cgatcaccct agccgcgagt gcgggcccgc ggcagggcca ccggccccct
     6001 cttccccggg ccatcgcggc cgggtggctg ccgtggaagt tgggtgagaa gccgacggga
     6061 ggagggaggc ctggatgggc ctccccccgt ccgtcctgac gacccgcgag cgctacgggg
     6121 gagtgaggat cgccgctccg aagatgctgg agtcgccgaa gttcgccgcg cagtcgttgt
     6181 tcggctccgg cctcggcgga tcgtccttgg tgctcttggt ccgcagggcc gcccgccacg
     6241 cgtcgatggc cgggcagtcc gcgcccgtcc ggacgtcgtt ccagacgaag gcggcgtagc
     6301 cgttcctcgc ggaggcgtac acgtagtcgc cgaggaactc ggcggtcagg tcgttctggc
     6361 tcgaggagcg ggcgtcgccc ggggtgctgc ggtgctgctc gctgaacgcc cccacggccc
     6421 cgccgaccac gtcggcgtgc gccacgacgg ccagcagcgg ccgcgggttc gcggtcgtcg
     6481 tctggtacgg cgcggtgaac gcgttgtagg cgatccaggc gtcctcgccg tccggcgaga
     6541 tggccgtggc cgtgtagtag ccgcggtcac ccgcgttcgt ctcgatcgag acgggcgccg
     6601 cccatgtgtc gccgccgtcg gtcgacgtcg agaacatcac gtgctcgtcg ttcagtgtct
     6661 ggccgtcgat ccagttcagc acgatctggt cggtggcgcc ggtcccgctc ggcgagccgt
     6721 tggcgatgtc cacgctcgga gaggtcgaca ggtcgctccg cgctccggcg atgccgtcca
     6781 tcgtgttgcg gccgatcacg gggtcgaaca ccccggtcgg gtggacgatg aacagcttcc
     6841 ggcgcatctc gaacgtcgcc ccgccgtcga aggagcggga caggaagatc gcctcggcct
     6901 tggtctgctg gttgaagccg tgccagaaca gatagacgtt ccccgcgctg tcggtccgga
     6961 tcgtgcaccc ggtcgccccg gcggccaggg cccaattccc ggaggcggag ttcgtgttgg
     7021 gctcgacgat cacgttcgac caggtgtcgc cgccgtcggt ggaacgcgcc agggtcaggc
     7081 gcaccgcgtt cgagcccgcg ctggggccgc cgacgaagtt gccgaagcag acg
//
LOCUS       DASPWO010000019         2826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_172249, whole genome shotgun sequence.
ACCESSION   DASPWO010000019 DASPWO010000000
VERSION     DASPWO010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172249"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VEO00_00430"
     CDS             <1..539
                     /locus_tag="VEO00_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HYU56501.1"
                     /translation="TIGILTTSWGEVLRDVLKRAEDEPLLADRLPAGWHRDPEGFAAE
                     ARGRIDELAAWLGKADTEELAARRTRRFLSTRRPLLAGGLEAIVALESVDDETILRRR
                     PGSVCEVRLDGDRLSVLLGDRELRMPARLEDAMRAVAGRESFRPADLATWLDPAGRLI
                     LARRLIREGLLLSAADGE"
     gene            529..1434
                     /locus_tag="VEO00_00435"
     CDS             529..1434
                     /locus_tag="VEO00_00435"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HYU56502.1"
                     /translation="MGSSAPDRCSSVSLALDEPLLGTASTVTSWMLVEQPGPWGEYAL
                     ERNRLPPDAAAAIRLRCRQHRIRAILIRRWGGFGPEGRRVYLAHTGEREPWLEAAVLP
                     DPADLSALLDADLAALGRGGRPGLGEPMDRPLYLVCTNGGRDPCCAERGRPLFRALEP
                     ALADRIWECSHIGGDRFAGNLVCMPSGVYFGRVRPEEALSIARAHDEGRLDLDHYRGR
                     TCYPFAVQAAESFLRRELGLEAIAAVTLRSAAGDDGALTAGFDTPAGERTVRVRVEPS
                     PDPRRLTCHGQPDQHPPRYELLAIG"
     gene            1484..1723
                     /locus_tag="VEO00_00440"
     CDS             1484..1723
                     /locus_tag="VEO00_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56503.1"
                     /translation="MSKMIQVRNVPDRLHRELVRRAKAGGRSLTDYIQDILERELARP
                     PAGEVFERVSRRPAVRLGRPAAELIAEERAGREAS"
     gene            1720..2133
                     /locus_tag="VEO00_00445"
     CDS             1720..2133
                     /locus_tag="VEO00_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYU56504.1"
                     /translation="MRLVVADASALVEYLLRTERSEPVRMAVEAPDTDLHVPALCDVE
                     VTAAVRRALLGGVLDERRAIDAVEDYLDLSLTTHGHTALLHRIIALRRNLSVYDAAYV
                     ALAEALGAALLTADRSLERAVRQQQLVEVVASDGR"
     gene            2332..>2826
                     /locus_tag="VEO00_00450"
     CDS             2332..>2826
                     /locus_tag="VEO00_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYU56505.1"
                     /translation="MSRPTSRLIDEIARDYRYLFAFMQESAWARRQGDPGICDFVVGN
                     PHEMPVPEFAQALARAAVPRDKDWFAYKLSEPPAVEAVRATLRRSHGLDVEHHDVLMT
                     SGAFAGLAAVLRAVVDPGDEVVFTVPTWFFYDPIIRSMGAMPVRVAARPEDWDLDVDA
                     IAAAI"
BASE COUNT          357 a         1034 c         1033 g          402 t
ORIGIN      
        1 tcaccatcgg gatcctgacg accagctggg gcgaggtcct gcgcgacgtc ctgaagcgag
       61 ccgaagacga gcccctgctc gccgaccgcc tgccggcggg ctggcaccgc gacccggagg
      121 ggtttgcggc ggaggcccgc gggcggatcg acgagctggc ggcgtggctc ggcaaggccg
      181 acaccgagga gctcgccgcg cggaggacgc gacggttcct ctccacccgg cggccgttgc
      241 tggccggcgg gctggaggcc atcgtcgccc tcgagtcggt cgacgacgag acgatcctgc
      301 gccggcggcc cggttccgtg tgcgaggtgc ggctcgacgg ggaccggctg tcggtcctgc
      361 tcggtgaccg cgagctgcgg atgccggccc gcctcgagga cgcgatgcgg gcggtggccg
      421 gccgggagtc gttccgcccg gccgacctcg ccacctggct cgaccccgcc ggccgcctga
      481 tcctcgcccg gcgcctgatc cgcgagggcc tgctgctctc cgccgccgat ggggagtagc
      541 gcgccggacc gctgttcctc ggtctcgctc gctctcgacg aaccactgct cgggacggcg
      601 tcgaccgtca cgtcgtggat gctggtggag cagccggggc cgtggggcga gtacgcgctc
      661 gagcggaacc gcctgccgcc cgatgcggcc gcggcgatcc gcctgcggtg ccgccagcac
      721 cggatccggg cgatcctgat tcggcgctgg ggcggcttcg gcccggaggg ccgccgcgtc
      781 tatctcgccc acaccggcga gcgggaaccc tggctcgagg ccgcggtgct gcccgacccg
      841 gccgacctct cggcgctgct cgacgccgac ctcgccgccc tcgggcgcgg agggcggccg
      901 gggctcggcg agccgatgga ccggccgctc tacctggtgt gcacgaacgg agggcgcgac
      961 ccctgctgcg ccgagcgcgg ccggccgctg ttccgcgcgc tggagccggc actggccgac
     1021 cggatctggg agtgctcgca catcgggggc gaccgtttcg ccggcaacct ggtctgcatg
     1081 ccgagcggcg tctacttcgg ccgcgtgcgg cccgaggagg ccctgtccat cgcccgcgcc
     1141 cacgacgagg ggcgcctgga cctcgaccac tatcgcggcc ggacctgcta cccgttcgcg
     1201 gtccaggccg ccgagtcgtt cctgcgccgc gagctgggcc tcgaggcgat cgccgccgtc
     1261 acgctccgct cggccgccgg ggacgacggc gcgctgaccg cgggcttcga caccccggcg
     1321 ggcgagcgca cggtgcgcgt gcgcgtcgag ccctcacccg acccccgccg cctgacctgc
     1381 cacggccaac ccgaccagca cccaccgcgc tacgagctgc tggcgatcgg ttgactgact
     1441 gattcccatt gaacatgtga tacgcatgtg acatgctatg cgcatgtcga agatgatcca
     1501 ggtcaggaac gtccccgatc ggctccatcg cgagctcgtc cgcagagcga aggccggtgg
     1561 tcggtcgctg accgactaca tccaggacat cctggagcgc gagctggcgc ggccccccgc
     1621 cggagaggtg ttcgagcggg tgagcaggcg cccggccgtg cggctcgggc gcccggcggc
     1681 cgagctgatc gccgaggaac gcgccgggcg ggaggcttcc tgaggctcgt cgtcgcggat
     1741 gcctcggccc tggtcgagta cctcctcagg acagagcggt ccgagcccgt caggatggcg
     1801 gtcgaggctc ccgacaccga cctgcatgtt cccgccctct gcgacgtcga ggtgaccgcc
     1861 gccgtgcgcc gtgcgctcct cggtggcgtc ctcgacgaac gccgtgccat cgatgccgtc
     1921 gaggactacc tcgacctctc cctgacgacc cacggccaca cggccctgct ccaccggatc
     1981 atcgccctgc gccggaacct ctcggtctac gacgccgcgt acgtggcgct cgcggaagca
     2041 ttgggcgcag ccctcctcac tgccgaccga tcgctggagc gagccgtccg acagcagcaa
     2101 ctcgtcgagg tcgtggcgtc ggacggtcgc tgacaacgct gcgcgcagcg gcgaggcgtt
     2161 acggggcgat gcagcggacg gcttcctgcc ggccgcaggg gatctcggga ccgaggctga
     2221 caccagtcag ggggaccatc gggagcagga tgcggctggg atgcgccgcg gcgtgctcgg
     2281 attccttccg ggggtggccg cccttcgccg atccggggga caatcggccg tgtgagccgg
     2341 ccaacgtcgc ggctgatcga cgagatcgct cgggactacc gctacctgtt cgcattcatg
     2401 caggagtccg cgtgggcgcg ccgccagggc gatccgggga tctgtgactt cgtcgtcggg
     2461 aacccgcacg agatgccggt cccggagttc gcgcaggccc tggcgcgggc cgccgtcccc
     2521 cgggacaagg actggttcgc ctacaagctc tccgaaccgc cggcggtcga ggccgtccgg
     2581 gccacgctgc gccggagcca cgggctggat gtcgagcacc acgacgtgct gatgacgagc
     2641 ggcgcgttcg ccggtctggc cgcagtcctc cgcgcggtcg tcgacccggg ggacgaggtc
     2701 gtgttcacgg tgccgacctg gttcttctac gacccgatca tccggtccat gggcgctatg
     2761 cccgtgcggg tggcggcccg gccggaggac tgggatctcg acgtggacgc gatcgccgcc
     2821 gcgatc
//
LOCUS       DASPWO010000020         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_681223, whole genome shotgun sequence.
ACCESSION   DASPWO010000020 DASPWO010000000
VERSION     DASPWO010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681223"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1532
                     /locus_tag="VEO00_00455"
     CDS             153..1532
                     /locus_tag="VEO00_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HYU56506.1"
                     /translation="MGKRKRRADRSGRAPLRSPGRPPVARREERQRFWVGIAEGLTSE
                     DAAVRAGVSPAVGARWFRQSGGMPTTSQAPLSGRYLSFAEREEIALWRARGCGVRAIA
                     RRLGRSPSTISRELRRNAATRGGGLDYRATTAQWHADRRARRPKTATLAANPALRRYV
                     QDRLAGAVTAADGTRVAGPAVRWIGRRHGRRQDRRWAAAWSPEQIAHRLAVDFPDDAS
                     MRISHEAIYQALYVQGRGALRRELTACLRTGRALRVPRARTRGTAKGFVSDEVLIRER
                     PAEADDRAVPGHWEGDLILGLGSSAIGTLVERTTRFTLLLHLPRLDGHGGPRGKNGPA
                     LAGHGAAAVRDAIASAVTTLPAQLRRSLTWDQGAEMAQHAQLRIDTDLRVSFCDPHSP
                     RQRGTNENTNGLLRQYFPKGTDLSRHGPDDLAAVAAALNGRPRKTLAWKTPAETLDDL
                     LVSGMSAWG"
     gene            1924..>2357
                     /locus_tag="VEO00_00460"
     CDS             1924..>2357
                     /locus_tag="VEO00_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56507.1"
                     /translation="MTLTRRLAAVALLLVAGAAVAFGFGGARPAAAEGTKSSGDYVVI
                     DVPDHLIDDLVAAQRGTSRSGGGWADLVLDLSYKFCGTYQCLCIDLDPDECYPDADQV
                     GDDRISLVAWLRTPAVVDLVLREKLCSTQQWLIEGCQVVGPAR"
BASE COUNT          311 a          888 c          839 g          319 t
ORIGIN      
        1 gagcggtggg gccgcgtgtc cgagcagacc ctgcgccagg ttgaggatcg cttgcggatc
       61 gtgctcgacc tggcttgaga tcatgacgct tgtcggggcg gactacccgg gttcaagcgg
      121 tcgtcgcaac accctgacgg aagggagttg cgatggggaa gcgcaagcga cgggcggatc
      181 ggtccgggcg cgcgcccctg cggtcgccgg gtcgtcctcc ggtggcccga cgggaggagc
      241 ggcaacgctt ctgggtgggc atcgccgagg gcttgacgag cgaggacgcc gcggtgcggg
      301 cgggggtgtc gccggcggtc ggggcgcggt ggttccggca gagtgggggc atgccgacga
      361 ccagtcaggc cccgctgtcg ggccgctacc tctccttcgc cgagcgcgag gagatcgcgc
      421 tctggcgcgc ccgcggctgc ggcgtccgcg cgatcgctcg gcgcctcggc cgctcgccat
      481 cgacgatctc gcgcgaactg cgacggaacg ccgcgacccg cggcggcggc ctggactacc
      541 gcgccacgac ggcccagtgg catgccgatc ggcgggcgcg gcgcccgaag acggccacgc
      601 tggccgccaa tccggcgctg cggcgctacg tgcaggaccg gctcgcgggc gccgtgacgg
      661 ccgcggacgg gacgcgcgtg gccggtccgg cggtgcgctg gatcggtcga cgccacgggc
      721 gccgccagga tcggcgctgg gcggcggcgt ggagccccga gcagatcgct catcggctcg
      781 cggtcgactt ccccgatgat gcgtcgatgc ggatctcgca cgaggcgatc taccaggcgc
      841 tgtacgtcca gggccgcggg gccctgcgcc gcgagctgac cgcctgcctg cgcaccgggc
      901 gggccctccg cgtcccgcgg gcccgcaccc gcgggaccgc caagggcttc gtcagcgacg
      961 aagtcctgat ccgcgagcga ccggccgagg ccgacgaccg ggccgtcccc gggcactggg
     1021 agggcgacct catcctcggc ctcggcagct ccgccatcgg caccctcgtc gagcggacca
     1081 cccggttcac cctgctcctg cacctgcccc gtctggacgg ccacggcggg cccagaggca
     1141 agaacggtcc ggcgctggcc ggccacggcg ccgcggccgt ccgcgacgcg atcgcctccg
     1201 ccgtcacgac gctgcccgcg cagctgcgcc gatcgctgac ctgggaccag ggcgccgaga
     1261 tggcccagca cgcccagttg cgcatcgaca ccgacctgcg ggtgtccttc tgcgaccccc
     1321 acagcccccg gcagcgcggc acgaacgaga acaccaacgg cctgctccgc cagtacttcc
     1381 cgaaaggaac ggacctcagc cgacacggcc cggacgacct cgccgccgtc gccgccgcgc
     1441 tcaacggccg tcctcgtaag acgctcgcat ggaagacgcc agcggagacc ctggacgatc
     1501 tcctcgttag cggcatgtct gcgtggggct gagacggccc gtttcggcag cgttcttcgc
     1561 ggctggcgtc gccgccggcc ggggtgtgcg cccgtcccca ggatggaaga ggaggatcgc
     1621 cgagcgatcc tcctccgtcc ggcacccagc gggggaacgc gtcaccgccg cagcggccgc
     1681 agcgcgcgca gcgagcggag gacccccggc agggggttga cgcgtggggg ccgcaggccc
     1741 ccctcctgag gtcgcccccc tgctccggct ccaccgcggc gccgctcacc gtcatcggca
     1801 tgcccacgga gttctgccga accatccccg tccaatcggg ccgcatgggg tgcgaactat
     1861 tcggcccgta acactgctac gctcatttca tcagggcgtt ccgacgaccc cttgaatccg
     1921 cccatgacac tgacccgtcg tctcgccgcc gtcgccctgc ttctcgtcgc gggcgcggcg
     1981 gttgccttcg gcttcggcgg cgcccggccg gccgccgccg aggggacgaa atcctccggc
     2041 gactacgtcg tcatcgacgt cccggaccac ctcatcgacg acctcgtcgc ggctcagcgc
     2101 ggcacctcac ggtcgggcgg cggctgggct gacctcgtgc tggacctctc ctacaagttc
     2161 tgcggcacct accagtgcct ctgcatcgac ctcgaccccg acgagtgtta cccggacgcc
     2221 gaccaggtcg gcgacgacag gatctcgctc gtggcctggc ttcggacgcc ggccgtcgtc
     2281 gacctggtcc ttcgggagaa gctgtgcagc acccagcagt ggctcatcga gggctgccag
     2341 gtagtgggtc ccgcccg
//
LOCUS       DASPWO010000021         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_2637, whole genome shotgun sequence.
ACCESSION   DASPWO010000021 DASPWO010000000
VERSION     DASPWO010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2637"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VEO00_00465"
     CDS             <1..294
                     /locus_tag="VEO00_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HYU56508.1"
                     /translation="ELPQLFNVLRGEMSIVGPRPERPHFVELFSDRYPQYYLRHRIPP
                     GITGWSQIHGLRGDTSIEERAVFDNHYIENWSFSRDVKIILRTVGTLVRKEGR"
     gene            297..587
                     /locus_tag="VEO00_00470"
     CDS             297..587
                     /locus_tag="VEO00_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HYU56509.1"
                     /translation="MRRAHAHPLAPIVRSIVAITVLVLMLLVAVPAHAQCDPYSGNCP
                     STEPPSDPGQGGVRARGGLAFTGADVTLFLVAGIAALGLGTILVRRSREPSA"
     gene            743..1048
                     /locus_tag="VEO00_00475"
     CDS             743..1048
                     /locus_tag="VEO00_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56510.1"
                     /translation="MTTERANPPLRRRLTALASLVVVLVAMLLLLAAPARAQCDPYSG
                     NCPSTGAPTDPGTGVRDRDGLAFTGADVTLFVLTGLLAIGTGAALVRRHRPNPDDAS"
     gene            complement(1080..2945)
                     /locus_tag="VEO00_00480"
     CDS             complement(1080..2945)
                     /locus_tag="VEO00_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HYU56511.1"
                     /translation="MRRRVLLAVAGAVVVVAALAALVAARTGTELAAARRLLDASVED
                     VGAADLREAGVHLRNARNRLHGPAALLLRAVPVLGTNLRALDAAAAAAIPALDTALEV
                     RGAVDRIQAEGLVTGGTVNLGAIAGLRAPLGREVEALAGLESEAADRKSGWLLPPVWD
                     ALDGLQRRAHDLRRSTEGLGRLLELAPRMLGQDTPRRYLVVLINNAELRGGGGLLAGV
                     GTVIARDGRLRVGRLYQAEELQTVPPETVPAPTEYERRFGPYQANTTLWKNTTFSPDV
                     PDDALVAARLFEHVTGTATEGAIVVDPRGLAALLPPDAALPIPGLDHPLGRDEFADFV
                     YSEAYRRFTDERERRASILAAGGAAFEAILGGGLLSEEGLGAAGVALAGGHLRIVSFE
                     AAEADVLEALGVSGRLESPEGDSLLVTVQNFGGGGREGSKMDFWARRQVEQACSLAPG
                     EDARCATTVRLRNVAPSGLPRYVAGTPYGLLRSYLETYVPEDAEVIGVVLDGEPAAHQ
                     VEPQAGRVAIGVYVEVPPGGTAEVEVRYVLPGSGQTYSLVALPQPLVRDADLLLALRL
                     PRDWEVIGPGAWDGDVWRYRGGFAGPVGILAGPDQRSGIPRIWDALTDFWRGPLF"
     gene            2968..>3741
                     /locus_tag="VEO00_00485"
     CDS             2968..>3741
                     /locus_tag="VEO00_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU56512.1"
                     /translation="MRVLHVSQPVEGGVGRWVAALVAHQRALGWDVVIAAPGAGELSA
                     RAAAAGAAVRSWEASRSPGASVPGEVRRLRRIVRAAAPDLVHLHSSKAGLAGRLALRG
                     RVPTVFQPHGWSFYAVTGATRRAAVVWERAGARWADRILCVSAGERARGSAARIRARW
                     AVIPNGTDVEAIALPTERARAALRPRLGVGPGPLVVFVGRIDRAKGPDVLLDAWPAVT
                     AAVPEARLMVVGEGPLLPALAARVPAGAGLAGHRDDPEAW"
BASE COUNT          453 a         1441 c         1387 g          460 t
ORIGIN      
        1 gagctcccgc agctcttcaa cgtgctgcgg ggggagatgt cgatcgtcgg tccacgcccc
       61 gagcggccgc acttcgtgga gctgttctcg gaccggtacc cgcagtacta cctgcgccac
      121 cgcatcccgc cgggcatcac cggctggagc cagatccacg gtctgcgcgg cgacacctcg
      181 atcgaggagc gcgccgtgtt cgacaaccac tacatcgaga actggtcgtt ctccagggac
      241 gtgaagatca tcctcagaac cgtcggaacc ctggtccgaa aggaaggacg gtagccatgc
      301 gccgcgccca cgcccacccg ctcgccccca tcgttcggtc gatcgtcgcg atcaccgtgc
      361 tcgtgctgat gctgctcgtg gccgtcccgg cccacgcgca gtgcgacccc tacagcggga
      421 actgcccctc gaccgaaccg ccctccgacc ccggccaggg cggcgtgcgg gcccgcggcg
      481 gcctcgcgtt caccggcgcg gacgtcacgt tgttcctggt cgcggggatc gcggccctcg
      541 gcctggggac catcctggtc cgccggtcgc gcgagccctc cgcctaggct ccggaggctg
      601 cgcgatggcc cgggatgtgt cccgccccgc caccatcggg tcgggcgccc tcgccccaaa
      661 cgcatggcgt ctcggctcaa cccggccgcc ccggctaccg aacgtaccga cgggggcacc
      721 ttccccgggg gagtgaggac cgatgaccac cgaacgagcg aacccaccgc tccgccgccg
      781 gctcacggcg ctggcgtcgc tcgtcgtcgt gctcgtggcg atgctgctgc tcctggccgc
      841 gcccgcccgg gcgcagtgcg acccgtacag cgggaactgc ccttcgaccg gcgcgccgac
      901 ggacccgggc accggggtgc gcgatcgcga cgggctggca ttcaccggcg ccgacgtgac
      961 gctgttcgtc ctgaccggcc tgctggccat cgggacgggt gccgccctcg tgcgccgtca
     1021 ccgcccgaac cccgacgacg cgtcgtagcg tccggctgaa ggccgggtcc cggcgtggtt
     1081 cagaagaggg ggccgcgcca gaagtcggtc agcgcgtccc agatcctcgg gatgcccgag
     1141 cgctggtcgg gcccggccag gatgccgacc ggcccggcga atccgccgcg gtagcgccag
     1201 acgtcgccgt cccacgcccc cggcccgatc acctcccagt cccgcggcag gcggagcgcc
     1261 aggaggagat ctgcgtcgcg cacgaggggc tgggggagcg cgacgagcga gtacgtctgg
     1321 ccgctcccgg gaaggacgta gcgcacctcg acctcggccg tgccgcccgg cgggacctcg
     1381 acgtagacgc cgatcgccac gcggccggcc tggggctcga cctgatgcgc cgccggctcg
     1441 ccgtcgagca cgaccccgat gacctcggcg tcctcgggaa cgtaggtctc gaggtagctc
     1501 cgcagcagtc cgtagggggt gccggcgacg taccgcggca ggccggacgg cgcgacgttc
     1561 cgcagccgaa ccgtcgtggc gcagcgcgca tcctcgcccg gcgccagcga gcaggcctgc
     1621 tcgacctgcc ggcgggccca gaagtccatc ttcgatccct ctcgcccgcc gccgccgaag
     1681 ttctgcaccg tgacgagcag ggagtctccc tcgggcgact ccagacggcc gctgaccccg
     1741 agcgcctcca gcacgtcggc ctcggccgcc tcgaacgaga cgatgcgcag gtgtccgccg
     1801 gcgagcgcca cccccgccgc ccccaggccc tcctcggaga gcaacccgcc gccgaggatc
     1861 gcctcgaagg ccgcgccgcc cgccgcgagg atggaggccc gccgctcccg ctcgtcggtg
     1921 aagcgccggt acgcctcgga gtagacgaag tcggcgaact cgtctcggcc gagcgggtgg
     1981 tcgagcccgg ggatcgggag ggcggcgtcg ggagggagga gcgccgccag cccgcgcggg
     2041 tcgacgacga tcgcaccctc cgtggccgtg ccggtgacgt gctcgaacag ccgcgccgcg
     2101 acgagggcgt cgtcggggac gtcgggcgag aacgtcgtgt tcttccacag cgtggtgttc
     2161 gcctgatagg gaccgaagcg gcgctcgtac tcggtcgggg ccggtaccgt ctcgggcggg
     2221 accgtctgga gctcctcggc ctggtagagg cgcccgaccc gcaggcggcc gtcccgggcg
     2281 atcacggtcc caaccccggc gagcaaccct ccacccccgc gcaattccgc gttgttgatc
     2341 agcaccacca ggtagcggcg gggcgtgtcc tgtccgagca tccgcggggc gagctccagc
     2401 aggcgcccga gcccttcggt ggaccggcgc aggtcatgag cccggcgctg caggccgtcg
     2461 agcgcgtccc acaccggcgg gaggagccac ccgctcttgc ggtccgccgc ctcggactcc
     2521 agcccggcca gcgcctccac ctcccgcccc agcggcgcgc gcaggccggc gatcgcgccg
     2581 agattgaccg tgccgccggt gacgagcccc tcggcctgga tgcgatcgac ggctccccgg
     2641 acctcgaggg ccgtatcgag ggccgggatc gccgccgccg cggccgcgtc caaggcccgc
     2701 aggttcgtcc cgagcaccgg gacggcgcgc aggaggagcg ccgccgggcc gtgcaggcga
     2761 ttgcgggcgt tccgcaggtg gacgcccgcc tcccgcaggt cggccgcccc gacatcttcg
     2821 acggaggcgt ccagcaggcg ccgggccgcg gccagctccg tcccggtgcg cgcggcgacc
     2881 agcgcggcga gggcggcgac gacgacgacg gcgccggcca cggcgaggag cacacggcgg
     2941 cgcacgacca tcgatactct acggcgggtg cgcgtgctcc acgtgtcgca gccggtggag
     3001 ggcggcgtgg gccgctgggt cgcggcgctg gtcgcccacc agcgcgcgct cggatgggac
     3061 gtggtgatcg ccgccccggg ggcgggtgag ctctccgctc gcgccgcggc cgccggcgcc
     3121 gcggtgcgct cctgggaggc gtcccggtcg ccgggggcct cggtgccggg cgaggttcgc
     3181 cgcctgcggc ggatcgtccg ggcggccgcc ccggacctcg tccacctgca ctcgtcgaag
     3241 gccgggctgg ccggccgcct ggcgctgcgc gggcgggtcc cgaccgtctt ccagccgcac
     3301 ggctggtcgt tctacgccgt caccggggcc acccggcgcg cggcggtcgt ctgggagcgg
     3361 gcgggggcgc ggtgggcgga ccgcatcctc tgcgtcagcg cgggggagcg ggcccgcgga
     3421 tccgccgctc gcatccgtgc gcgctgggcg gtgatcccga acggcaccga cgtcgaggcg
     3481 atcgcgctcc ccacggagag ggcgcgcgcc gcgctgcgcc cccggctggg ggtcgggccc
     3541 ggcccgctcg tcgtcttcgt cggacgcatc gatcgggcca agggccccga cgtgctgctc
     3601 gacgcctggc cggcggtcac ggccgcggtc ccggaggccc ggctgatggt cgtcggggag
     3661 gggccgctcc tcccggcgct cgccgcgcgg gtcccggcgg gcgccgggct ggcgggccac
     3721 cgggacgacc cggaggcgtg g
//
LOCUS       DASPWO010000022         5546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_2714, whole genome shotgun sequence.
ACCESSION   DASPWO010000022 DASPWO010000000
VERSION     DASPWO010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2714"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VEO00_00490"
     CDS             complement(<1..352)
                     /locus_tag="VEO00_00490"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HYU56513.1"
                     /translation="MWFSDLEPERLDADALVLLAPSAYVKSWLSSHYGELLSTVVARA
                     AGEPLRVDLDVATAGRRTGGAPADAPASADQAAEEPGPARGETELGTPFATRYTFDSF
                     VPGPSNKFAHAAAMA"
     gene            701..835
                     /gene="rpmH"
                     /locus_tag="VEO00_00495"
     CDS             701..835
                     /gene="rpmH"
                     /locus_tag="VEO00_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007054596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HYU56514.1"
                     /translation="MKRTFQPNNRRRHKKHGFRVRMRTRAGRAILARRRAKGRTRLSA
                     "
     gene            854..1180
                     /gene="rnpA"
                     /locus_tag="VEO00_00500"
     CDS             854..1180
                     /gene="rnpA"
                     /locus_tag="VEO00_00500"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HYU56515.1"
                     /translation="MPATLRSSADYRRVLGVGTATHGTLVVLYVALSEGPSRAGFVAG
                     RRVGGAVTRNRARRLLREAWRVAGPRVREGHDIVLVARAAIREAKAQDVIAETQDLLA
                     RAGMLR"
     gene            1177..1509
                     /gene="yidD"
                     /locus_tag="VEO00_00505"
     CDS             1177..1509
                     /gene="yidD"
                     /locus_tag="VEO00_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003783774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HYU56516.1"
                     /translation="MSHAALFLRRALWVAGWPARVTLLALIGTYRALISPFLGSRCRF
                     HPSCSAYAEEAVRVHGAVKGSALAAWRVLRCSPLTAGGLDPVPARGGSARRGTPQYDD
                     VIQKAGSS"
     gene            1506..2555
                     /locus_tag="VEO00_00510"
     CDS             1506..2555
                     /locus_tag="VEO00_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HYU56517.1"
                     /translation="MIAVAWWQSILSAFGSVLSFLYEIVANYGLAIVLLTLLVRVILL
                     PLTIKQTRSMQEMQRVQPLVKELQKKYKGDRQKLNEEMMKLYREHRVNPLGGCLPLLL
                     QFPVFIALYSVLRGDPKLAAAKFAGRFTGQILPSIAHLPVGSSLATAIQNGKAGFLGM
                     NLSCAPLQAGKGSFAIVQGGAKVLNCGTTWPAAIPFFVMVALMVLTTWYQQRQMQAMS
                     GQSNPQMRLMGRIMPVFLGFISLQIPAGVLLYWVTTNFWQVGQQTVMLRMRAREGGAP
                     LPARRDGRADGRGDGKPGGPSGKPAGRPAPKAAPGRPKQPPSPTPLPPGELSRRSSPG
                     VTRSRSGSRKKRRKR"
     gene            2524..3189
                     /gene="jag"
                     /locus_tag="VEO00_00515"
     CDS             2524..3189
                     /gene="jag"
                     /locus_tag="VEO00_00515"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HYU56518.1"
                     /translation="MEVERSAASVEEALEAALSELGVTEQEAEIEILQEPRSGFLGLK
                     QQDAIVRVRARRAALPELTEEELEEQADIAADFLEELFDMMELDADVETNLVDGVMYV
                     DVWALEDEDEMGILIGKHGATLDAVQELVRGVVNRETEQRCRVMVDVEDYRKRRRSQI
                     AERAANVAHRVKRSGRPEALEPMNAWERKVVHDAVAKLGGGLETASEGEDPERRVVIR
                     RSG"
     gene            3271..3888
                     /locus_tag="VEO00_00520"
     CDS             3271..3888
                     /locus_tag="VEO00_00520"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HYU56519.1"
                     /translation="MKRRDLVAEAASTLGLALTDAQVGALLGFADRLAGRGIEIGAVA
                     RGDRDRILERHVLDSLRATPFVRPEDRIAYDFGSGGGLPGVPLAVACPWTTWVLVESR
                     HWRAAFLELVADELGLNVRVERSRVEDMAVPADLATSRAFAPLGRAWELARPRLKPGG
                     RLVFFAGARMTDPEADLSGRGVHGSVDVIPDVLDRGGALVIIERP"
     gene            4367..5116
                     /locus_tag="VEO00_00525"
                     /pseudo
     CDS             4367..5116
                     /locus_tag="VEO00_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017531359.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            5305..>5546
                     /locus_tag="VEO00_00530"
     CDS             5305..>5546
                     /locus_tag="VEO00_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HYU56520.1"
                     /translation="MTAKRGGLGRGLSALIPGATEESGLLEVPVHAVAPNPRQPREGF
                     DEEALAALARSIQEVGVLQPIVVRLRDGGYELVAGER"
BASE COUNT          797 a         1951 c         2006 g          792 t
ORIGIN      
        1 ccgccatcgc cgccgcgtgc gcgaacttgt tgctcgggcc ggggacgaag gagtcgaacg
       61 tgtagcgggt ggcgaacggg gtcccgagtt ccgtctcgcc ccgtgccggt ccgggctcct
      121 cggccgcctg gtcggccgac gcgggtgcat ccgccggcgc gccgccggtg cgccgcccgg
      181 ctgtggccac gtcgaggtcg acgcgcagcg gctcgccggc cgcgcgggcc acgacggtgg
      241 acagcagctc gccgtagtgc gaggacagcc aggacttcac gtaggcgctc ggggcgagca
      301 gcaccagcgc gtcggcatcg agccgctccg gctccagatc ggagaaccac atctcgaacg
      361 agctgtcgga gagctcctgg cgggccaggt cgagcgcacg ggaccagacc ccggttgcgg
      421 aacgttcaga cgtgcttgag gactgctttt ccacaggcac tttccacagt tgtggactcg
      481 tttcgcgcgg cgggggaacg tcctgcgaag cggccgggcc tggggaagga actcggcgca
      541 gtgtagcacc ggcccccacc cccctcgacc gcccttctcg cgttttggcg aacgtttttc
      601 cccactccgc cgttgccggc gccacttggg agcgcagccg tccagcccct atccagcccc
      661 tatactgacc gcccgatcgt catcgccgag ggatcgccac gtgaagcgca ccttccagcc
      721 gaacaaccgc agacgtcaca agaagcacgg gttccgcgtg cggatgcgca cgcgagccgg
      781 ccgcgccatc ctggcgcggc gccgggccaa gggacggacc cggctgtcgg cctaggcgcc
      841 accaccgcac ctgttgccag cgaccctgcg aagctcggcg gactaccgcc gcgtgctcgg
      901 cgtcggtacc gccacccacg ggaccctggt ggtactgtac gtggcgctct cggaggggcc
      961 ttcgcgcgcc ggcttcgtcg ccggtcgccg cgtgggcggg gcggtcaccc gcaaccgcgc
     1021 ccggcggctg ctgcgtgagg cgtggcgggt cgccgggccg agggtacggg aaggacatga
     1081 catcgtgctc gtggcccgag cggccatccg tgaagccaag gcccaggacg tgatcgccga
     1141 aacccaggac ctcctcgcgc gcgccgggat gctgcggtga gccacgccgc cctgttcctt
     1201 cgccgggccc tgtgggtcgc ggggtggccg gcccgcgtca cgctgctggc gctgatcggg
     1261 acctaccgcg cactgatctc cccgttcctc ggctcgcggt gccgcttcca cccctcgtgc
     1321 tcggcctacg cggaggaggc ggtccgcgtt cacggcgcgg tgaaggggtc ggcgctggcc
     1381 gcctggcgcg tcctgcgttg ctcgccgctc accgcgggcg gcctcgaccc ggtccctgcg
     1441 cggggcggat cggcccgccg gggaacacct cagtatgatg acgtcataca gaaggccggc
     1501 tcttcgtgat cgccgtcgcc tggtggcagt cgatcctgtc cgcgttcggg agcgtgctgt
     1561 cgttcctgta cgagatcgtg gcgaactacg gcctcgccat cgttctgctg acgctgttgg
     1621 tacgggtcat cctgctgccg ctgacgatca agcagacccg atcgatgcag gagatgcagc
     1681 gggtccagcc cctggtgaag gaactgcaga agaagtacaa gggcgaccgc cagaagctca
     1741 acgaagagat gatgaagctg taccgggagc accgggtcaa tccgctcggt gggtgcctcc
     1801 cactgctgct gcagttcccg gtgttcatcg ccctgtactc ggtcctgagg ggcgatccca
     1861 agctggccgc cgccaagttc gccgggaggt tcaccgggca gatcctcccc tcgatcgccc
     1921 acctcccggt gggcagctcg ctcgctaccg ccatccagaa cggcaaggcc ggcttcctgg
     1981 ggatgaacct ctcctgcgcg ccgctgcagg cgggcaaggg gagcttcgcc atcgtccagg
     2041 gcggggccaa ggtcctgaac tgcggcacca cctggcccgc cgccatcccg ttcttcgtca
     2101 tggtcgcgct gatggtgctg accacctggt accagcagcg ccagatgcag gcgatgtccg
     2161 ggcagagcaa cccgcagatg cggctgatgg gccggatcat gccggtgttc ctggggttca
     2221 tctcgctgca gatcccggcc ggcgtgctgc tgtactgggt gacgacgaac ttctggcagg
     2281 tcgggcagca gaccgtgatg ctgcggatgc gcgctcgcga gggcggcgcc ccgctccccg
     2341 cgcgtcgaga cgggcgggcg gacggccgtg gcgacgggaa gcccggcggg ccgtccggca
     2401 agcccgcggg aaggcccgcc ccgaaggcgg ccccaggccg gccgaagcag cccccctcac
     2461 ccactcccct cccacctggc gagctatcgc gccggtccag ccccggcgtg acccggagtc
     2521 gaagtggaag tcgaaagaag cgccgcaagc gttgaggagg cgctcgaggc ggccctttcc
     2581 gagctcggcg tcaccgagca ggaggccgag atcgagatcc tccaggagcc ccgcagcggc
     2641 ttcctcggcc tgaagcagca ggacgcgatc gtccgcgtgc gtgcccgccg cgcggctctc
     2701 ccggagctga ccgaggagga gctggaggag caggccgaca tcgcggccga cttcctggag
     2761 gagctcttcg acatgatgga gctcgacgcc gacgtcgaga ccaacctggt ggacggggtg
     2821 atgtacgtcg acgtctgggc cctcgaggac gaggacgaga tggggatcct gatcggcaag
     2881 cacggggcca cgctcgacgc ggttcaggag ctcgttcggg gcgtcgtcaa ccgcgagacc
     2941 gagcagcgtt gccgggtcat ggtggacgtc gaggactacc gcaagcgccg ccgctcccag
     3001 atcgccgagc gcgcggcgaa cgtggcccac cgcgtcaagc gctcgggccg gccggaggcg
     3061 ctggagccca tgaacgcctg ggagcgcaag gtggtccacg acgcggtggc gaagctgggc
     3121 gggggactgg agaccgccag cgagggggag gatcccgagc gccgcgtcgt gatccgccgc
     3181 agcgggtgac cacccaccgt ttcacgtgaa acatgcgcca gtgctcgtga cgggactccc
     3241 tggcgagtcc aacgctgcgc tccgtttcac gtgaaacggc gggacctggt cgccgaggca
     3301 gcttccacgc tgggcctggc actgaccgac gcgcaggtcg gggcgctgct cggctttgcc
     3361 gaccgcctcg ccgggcgcgg gatcgagatc ggagccgtcg cccgcggcga ccgggacagg
     3421 atcctcgagc gccacgtcct ggactcgttg cgggcgacgc cgttcgtccg gccggaggat
     3481 cgcatcgcct acgacttcgg ctccggcggg ggacttcccg gcgtgccgct cgcggtggcc
     3541 tgcccatgga ccacgtgggt cctggtcgag tccaggcact ggcgtgccgc tttcctcgag
     3601 ctggtggcag acgagctggg gctgaacgtc cgggtggagc gttcccgggt cgaggacatg
     3661 gccgtcccgg ccgacctggc aacctcgcgc gccttcgccc cgctgggcag ggcatgggag
     3721 ctggcacgcc cgcggctgaa gccgggtggg cgcctggtct tcttcgctgg agctcggatg
     3781 acggaccccg aagctgatct gtccggccgc ggcgtgcacg gatcggtcga cgtgatcccg
     3841 gacgtgcttg atcgcggcgg cgcactcgtt atcattgagc gtccctgact tccccgaaca
     3901 ggtccgggag gaccgagcca tccagtaccc cgccgccgtc tcctccggcg acaacgccgg
     3961 cgaccgtccc gacggtcccc atccctcgac gggcacgtcc gctgcgggcg gttccgcccg
     4021 accggacggt cgggatgcgg cctggtccga ggcagaccct gcgggcaggg cgctcgagca
     4081 ggcggacgag cccgcaaccg ccgagcccgc cgccgccgag cgggccacgg gccacccggc
     4141 cgtccgcgag cccttcgccg gccacccctt ggtcggcgat ccagtcggcg ggcccccggt
     4201 cgccccgggc gctccactgc ctccgtcccc ggcgactccc gccgagcagg tgccggcgcc
     4261 gaccaccgcg ggtgcagacg atctgccgga agcggcgggg gcggcatccg gcgagaaggc
     4321 ggagttcgac gaggaggact ttcgacctca ggtcaaggct tcacgcgtga tcgcgatcgc
     4381 caaccagaag ggtggcgtcg ggaagagcac gacggcggtc aacctcggcg ccgccctcgc
     4441 ggagctcggg cagcgggtcc tggtggtgga cctcgaccct caggggaacg cgtccaccgg
     4501 gctcgggatc gacgtggccg cgcgggaggt cagcacctac caggtcctga cccagggcgt
     4561 cccggcggag gacgcggtgg tggacaccgc gatcgagaac ctgtcggcgc tgccgtcgac
     4621 catcgatctg gccggcgcgg agatcgagct ggtcagccag ttctcccggg agatgcgcct
     4681 gtccaaggcc ctcgacccga tcgtcgccgc gtacgacatc gtcatcctcg actgcccgcc
     4741 gtcgctcggc ctgctgacgg tgaacgccct cgccgccgcc gacgagctga tcgtcccgat
     4801 ccagtgtgag tactacgcgc tcgagggact cggacagctg ctgcggaacg tgcgcctggt
     4861 gcagcagaac gtgaacccga acctcgtact cgcgggcatc gtgctgacga tgttcgactc
     4921 ccggaccaag ctggccgaac aggtcgtcgc cgaggtccgg aagtacttcg gcgggcgcgt
     4981 gtatgataat atcataccac gcaccgtgcg cctgtccgag gcccccggct acgggcagcc
     5041 gatcaccacc tacgacccct cgtccaaggg ggcggcggcc tatcgaggcc tggcgcgcga
     5101 ggtcctggcc ggcgaggggg agctcggcga tctcccgccc gaggattcca tgccggccgt
     5161 gatcgtgccg gagcgcacgc ccgaggacca gcaggcccac cgccgcgacg gggagcggcc
     5221 gttccgtagc cggcaccgcc gccgccggct gttcgggcgc aggggcgacg acgaggagca
     5281 ctcacccagg gaaggaggcg cggcgtgacg gcgaagcgtg gagggctggg gcgcggcctg
     5341 tcggcgctca tccctggggc gaccgaggag agcgggctcc tggaggtccc ggtgcacgcg
     5401 gtcgccccga accctcgaca gccgcgtgag ggtttcgacg aggaggccct ggcggcgctg
     5461 gcgcgctcga tccaggaggt cggggtgttg cagccgatcg tcgtccggct gcgcgacggc
     5521 ggctacgagc tggtggcggg ggagcg
//
LOCUS       DASPWO010000023         4865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_767132, whole genome shotgun sequence.
ACCESSION   DASPWO010000023 DASPWO010000000
VERSION     DASPWO010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767132"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..716
                     /locus_tag="VEO00_00535"
     CDS             60..716
                     /locus_tag="VEO00_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU56521.1"
                     /translation="MSLRVLLLDDHRMFADALEVLLAGESDIEVMGIATTEAEAIEVC
                     LRDCPDVVLVDLDPSGTDGFEATARIREACGHTQAVIVTGAYEPAMMARAVEAGATGY
                     VPKTGTARDLIDAVRRVASGELALPSADVNSVLRALQASHRTRTAGDLLAGHLTEREI
                     QILQGFADGLTTDELAGKLFVTTSTVQTHTRNILAKVGARSKLEAVIFALRHGVIRVR
                     "
     gene            893..1915
                     /locus_tag="VEO00_00540"
     CDS             893..1915
                     /locus_tag="VEO00_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56522.1"
                     /translation="MKRVALLASLALGVGLLSGTASANTTVTTPNGTWVAHPGQSSVS
                     QTSVQQPMNADGSSNFKHNSKAVIPVKFGLSTGTGPFVFESIWSDNPATTANDYSFLT
                     WIPDQPLTFAEVAELSAGYAFTDGDCAGGSLRWTVYLHDGGTTRNLDVHYQPGADGLS
                     QQICAAGTSGQNLIGSTDTIYVTQEFNGGYSFPSAYNNTYADAVSQLGGLTVEGMSLI
                     VDSGWGANGDQVVDLTNATVGVNNASPYTETFTPQPASALSPTCNLPQASIKVTKLSP
                     TPSGAVNDATSIQPFDDNELFRVVDCKYMYNLATSSLLGAGRYRVEAVINGTPATGAA
                     EFDLRA"
     gene            2133..2351
                     /gene="infA"
                     /locus_tag="VEO00_00545"
     CDS             2133..2351
                     /gene="infA"
                     /locus_tag="VEO00_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HYU56523.1"
                     /translation="MAKKDAIEVEGTVVEPLPNAMFRVELENGHRVLTHISGKMRMHY
                     IRILPGDRVLVELSPYDLTRGRIIYRYR"
     gene            2360..2473
                     /gene="rpmJ"
                     /locus_tag="VEO00_00550"
     CDS             2360..2473
                     /gene="rpmJ"
                     /locus_tag="VEO00_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HYU56524.1"
                     /translation="MKVRPSVKRMCEKCKVVRRRGRVVVICSNPRHKQRQG"
     gene            2477..2854
                     /gene="rpsM"
                     /locus_tag="VEO00_00555"
     CDS             2477..2854
                     /gene="rpsM"
                     /locus_tag="VEO00_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HYU56525.1"
                     /translation="MARIAGIDLPREKGVDVALRYIYGIGPTRAADTVSGANITEGTK
                     VRDLSEEEVVRIREWIDRNYKVEGDLRRDVAQDIKRKIEIGSYQGIRHRRSLPVRGQR
                     THTNARTRKGPKKTVGVRRKKGK"
     gene            2859..3251
                     /gene="rpsK"
                     /locus_tag="VEO00_00560"
     CDS             2859..3251
                     /gene="rpsK"
                     /locus_tag="VEO00_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011207365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HYU56526.1"
                     /translation="MAKPKQKKARKREKKNISHGQAHIKSTFNNTIVTITDLEGNTLS
                     WASAGNVGFKGSRKSTPFAAQLAAEQAARRAQEHGLQRVDVFVKGPGSGRETAIRSLA
                     AAGLEVIGIQDVTPVPHNGCRPRKRRRV"
     gene            3252..3878
                     /gene="rpsD"
                     /locus_tag="VEO00_00565"
     CDS             3252..3878
                     /gene="rpsD"
                     /locus_tag="VEO00_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HYU56527.1"
                     /translation="MARYTGPDCRLCRREREKLYLKGRRCESPKCAIEKRPYPPGEHG
                     RTRIKESEYLMQLREKQKARRIYGVLERQFRGYYQAAASQPGVTGENLLRLLETRLDN
                     VVYRAGLATSRDQARQLVRHKHFLVNGRSVNVPSFRIRPGDRVEVRQRSRNLLIVHDA
                     VEASESRVIPPWLDVDRGALAVTVTDLPSRTAIDTKLREQLIVELYSK"
     gene            3952..>4865
                     /locus_tag="VEO00_00570"
     CDS             3952..>4865
                     /locus_tag="VEO00_00570"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HYU56528.1"
                     /translation="MLIVARPHIEEDVVSPTRSRFTVEPLEPGFGYTLGNALRRTLLS
                     SIPGAAIASVRIDSVLHEFSTIPKVTEDVTDIILNLKQLVIRSEVDEPVTVYLRAKGP
                     GAVTAGDINPPAGVEILNPDLHIATLARGADLEMEMVVERGVGYVPAERNKKPRDPIG
                     VIPVDSIFSPVRRVTYSVENTRVEQMTDRDRLILDVQTDGSITPREAVASAGATLSEL
                     VALFSELTESQGISLGPAEEEALSGDLALTIEELNLSVRSYNCLKREGINTVGELVQK
                     SEAELMDIRNFGQKSIDEVKAKLEELGLS"
BASE COUNT          848 a         1711 c         1643 g          663 t
ORIGIN      
        1 acccttgacc gcgggtccac cgattgggga tcattcgtcc gtccgacccc aacacgccca
       61 tgagcctgcg cgtcctcctc ctcgatgacc accggatgtt cgccgacgcc ctcgaggtcc
      121 tcctcgccgg cgagtcggac atcgaggtca tgggcatcgc aaccacggag gcggaggcga
      181 tcgaggtctg cctccgcgac tgcccggacg tcgtcctcgt cgacctcgat ccgtcgggga
      241 cggacggctt cgaggccacc gcgcggatcc gggaagcctg cgggcacacg caggccgtga
      301 tcgtcaccgg cgcctacgag ccggcgatga tggcccgagc cgtggaggcc ggcgccaccg
      361 ggtacgtgcc gaagacgggg accgcgcgcg acctcatcga cgcggtgcgg cgagtggcgt
      421 cgggggagct ggccttgccg tcggccgacg tgaactcggt gcttcgcgca ctgcaagcgt
      481 cgcaccggac gcggaccgct ggcgatctgc tggccgggca cctcaccgag cgggagatcc
      541 agatcctgca ggggttcgcc gacggcctga ccacggacga gctggccggg aagctgttcg
      601 tcaccacgag cacggttcag acgcacaccc ggaacatcct ggccaaggtg ggggctcgct
      661 cgaagctcga agcggtgatc ttcgccctcc gccacggcgt gatccgcgtc cggtagccca
      721 gccgcccgcc cgcccgggcc cctgtcggtc ccagtgacta gtcagggcga atcagcgaaa
      781 accgcccgat cggcccaccg ctcgtaccgc gttcgtacga tgccgcgccg ccggcggaac
      841 cggacaatct ccatgcatcg acccgcgttg cacggagaaa gggaaaccct caatgaagag
      901 agtcgcgctt ctcgcgtcgc tcgcactcgg cgtaggcctg ctgtcgggaa cggcatcggc
      961 caacacgacc gtcacgacgc ccaacggcac gtgggtggcc cacccgggcc agtcctccgt
     1021 ctcgcagacg tccgtccagc agccgatgaa cgcggacggc agcagcaact tcaagcacaa
     1081 cagcaaggcc gtcatcccgg tgaagttcgg gctgtcgacc ggtaccggcc cgttcgtgtt
     1141 cgagtcgatc tggtcggaca acccggccac gacggccaac gactactcgt tcctgacctg
     1201 gatccccgac cagcctctga cgttcgcgga ggtggccgag ctgagcgccg gctacgcgtt
     1261 cacggatggt gattgcgccg gtggctccct ccgctggacc gtgtacctcc acgatggggg
     1321 aacgacccgg aacctggacg tccattacca gcccggggcg gacggtctct cgcagcagat
     1381 ctgcgcggcc ggcaccagcg ggcagaacct gatcgggtcc acggacacga tctacgtcac
     1441 gcaggagttc aacgggggct acagcttccc ctccgcctac aacaacacct acgcggacgc
     1501 ggtcagccag ctcggcggcc tgacggtcga aggcatgtcg ctgatcgtcg acagcgggtg
     1561 gggcgccaac ggcgaccagg tggtcgacct gacgaacgcc accgtcggcg tgaacaacgc
     1621 aagcccctac acggagacgt tcacgccgca gccggcctcg gcgctgtcgc cgacgtgcaa
     1681 cctcccgcag gcgtcgatca aggtcacgaa gctctcgccg actccatcgg gggccgtcaa
     1741 cgacgcgacg agcatccagc ccttcgacga caacgagctg ttccgcgtgg tcgactgcaa
     1801 gtacatgtac aacctcgcga cgagctcgct gctcggggcg gggagatacc gcgtggaggc
     1861 cgtgatcaac ggcaccccgg ccaccggcgc ggcggagttc gacctccgcg cctagggctc
     1921 gctcgagcgc cccacgcccg cggagagagg gccggccttc gggccggccc tctctcgttt
     1981 ccgctgctag actgcgtctt cgtcccgccc gaggtccggt ggacgctccg agcacgggcc
     2041 cgtatactct cctgttgggc ccggggcacg ccccggcgct ttcttctgac cgtgatcgac
     2101 gagaccccga ggcgaggagc tgaactcggc gcgtggccaa gaaggacgcg atcgaagtcg
     2161 aaggcactgt ggttgagccg ttgcccaacg ccatgttccg ggtggagctg gagaacggac
     2221 accgggtcct cacccacatc tcggggaaga tgcggatgca ctacatcagg atcctgccgg
     2281 gggaccgggt gctcgtggag ctctcgccct acgacctgac ccgcggccgg atcatctacc
     2341 ggtaccgctg aggcgcgcga tgaaggtccg accgtccgtc aagaggatgt gcgagaagtg
     2401 caaggtggtc cgccgtcgcg ggcgggtggt cgtgatctgc tcgaaccccc ggcacaagca
     2461 gaggcaggga tagcggatgg cacggatcgc cggcatcgac ctcccccgtg agaagggggt
     2521 ggacgtcgcg ctgcgctaca tctacgggat cggcccgacg cgcgccgccg acacggtgtc
     2581 cggcgccaac atcaccgagg ggacgaaggt ccgcgacctc tccgaggagg aggtcgtccg
     2641 gatccgcgag tggatcgacc gcaactacaa ggtcgagggc gacctgcgcc gcgacgtcgc
     2701 gcaggacatc aagcgcaaga tcgagatcgg ctcctaccag gggatccgcc accgccggag
     2761 cctcccggtg cgcggccagc gcacgcacac gaacgcccgc acgcgcaagg ggccgaagaa
     2821 gaccgtcggc gtgcgccgga agaagggcaa gtaaccgcat ggcgaagccg aagcagaaga
     2881 aggcccggaa gcgggagaag aagaacatct cccacggcca ggcgcacatc aagtcgacgt
     2941 tcaacaacac gatcgtcacg atcaccgacc tcgaggggaa caccctgtcg tgggcgagcg
     3001 ccggcaacgt cgggttcaag ggctcccgca agtccacccc gttcgccgcg cagctcgccg
     3061 ccgagcaggc cgcccgtcgt gcccaggagc acggcctgca gcgggtggac gtcttcgtca
     3121 agggcccggg gtccgggcgg gagaccgcga tccggtcgct cgccgcggcc gggctcgagg
     3181 tgatcggcat tcaggacgtc acgcccgtcc cgcacaacgg gtgccggccc cggaagcggc
     3241 ggcgagtgta gatggcgcgc tacacgggtc cggactgcag gctctgccgc cgcgagcggg
     3301 agaagctcta cctgaagggc cgccgctgcg agtcgccgaa gtgcgccatc gagaagcggc
     3361 cctacccgcc cggcgagcac ggccgcaccc ggatcaagga gtccgagtac ctgatgcagc
     3421 tgcgcgagaa gcagaaggcg cgccgcatct acggggtgct ggagcggcag ttccgggggt
     3481 actaccaggc ggccgccagt cagcccgggg tcaccggcga gaacctgctg cggctgctcg
     3541 agacgcgcct ggacaacgtc gtctaccgag ccggcctggc cacctcccgc gaccaggccc
     3601 gccagctcgt ccgccacaag cacttcctgg tcaacggccg ctccgtgaac gtcccctcgt
     3661 tccggatccg gccgggggac cgggtcgagg tccggcagcg ctcgcggaac ctcctgatcg
     3721 tccacgacgc cgtcgaggcg tccgagtcgc gggtcatccc accctggctc gacgtggacc
     3781 ggggggcgct cgccgtcacg gtgaccgacc tgcccagccg gaccgccatc gacacgaagc
     3841 tgcgggagca gctgatcgtc gagctctact cgaagtagcc ccgtgcccgc tgggggcgcg
     3901 ggaggaaccg acaccgggag aaacggatga tccccggcag gaggtagcac cgtgctgatc
     3961 gtcgccaggc cccacatcga ggaagacgtc gtctcgccca cccgctcccg gttcaccgtc
     4021 gagccgctcg agcccggctt cgggtacacg ctcgggaacg ccctgcgccg gacgctcctg
     4081 tcgagcattc ccggcgcggc catcgcgtcg gtgcgcatcg acagcgtgct gcacgagttc
     4141 tcgacgatcc cgaaggtcac cgaggacgtc accgacatca tcctgaacct gaagcagctg
     4201 gtgattcgct ccgaggtcga cgagccggtg acggtctacc tgcgggcgaa gggccccggg
     4261 gcggtcaccg ccggagacat caacccgccg gccggcgtcg agatcctgaa cccggacctg
     4321 cacatcgcca cgctggcgcg cggcgccgac ctggagatgg agatggtggt cgagcgcggc
     4381 gtgggctacg tcccggccga acggaacaag aagccgcgcg acccgatcgg agtgatcccg
     4441 gtcgactcga tcttctcgcc ggtccgccgc gtgacgtact cggtcgagaa cacccgggtc
     4501 gagcagatga ccgaccgcga ccgcctgatc ctcgacgttc agaccgatgg ctcgatcacg
     4561 ccgcgcgagg ccgtggcctc cgccggcgcg acgctctccg agctcgtggc gctgttctcc
     4621 gagctcaccg agagccaggg catctcgctc ggcccggccg aggaggaggc gctctccggc
     4681 gacctggcgc tgacaatcga ggagctgaac ctgtcggtcc gctcctacaa ctgcctgaag
     4741 cgcgagggga tcaacaccgt cggcgagctg gtccagaagt ccgaggccga gctgatggac
     4801 atccgcaact tcgggcagaa gtccatcgac gaggtgaagg ccaagctcga ggagctcggc
     4861 ctgtc
//
LOCUS       DASPWO010000024         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1022296, whole genome shotgun sequence.
ACCESSION   DASPWO010000024 DASPWO010000000
VERSION     DASPWO010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022296"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1555
                     /locus_tag="VEO00_00575"
     CDS             <1..1555
                     /locus_tag="VEO00_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYU56529.1"
                     /translation="IAGLRGDGGAATRLAAATDPAVAELLEAHPPRSRTRSPRLRIRV
                     DRPRALYGAWYELFPRSEGATETSSGTLRQAAERLPAIAGMGFDVVYLPPIHPIGFTN
                     RKGRNNSLAPRRDDPGSPWAIGGEEGGHTAVHPDLGTLEDFRRLVARARELGLDIALD
                     FAIQCSPDHPWLKEHPEWFHHRPDGTLKYAENPPKRYQDIYNVNWETEDRQGLWEAIR
                     DVVFHWMSHGVTVFRVDNPHTKPFAFWEWVIDDLKRRAPETIFLSEAFTRPRVLYRLA
                     KLGFTQSYTYFAWRRDRAELTEYFTELTASEVREFCRPNLWPNTPDILTEELQQGGRA
                     AFVARLVLAATLGASYGIYGPVYELCEARAVRPGSEEYLDSEKYQLRHWDLDRPDSLA
                     DLIARVNRIRRQNAALQADWSLRFHGTDNDRLLCYSKATADGSNAILVVVNLDHQHPQ
                     SGWTALWMEALGLEEGAEFQVHDLISEGRYRWRGPHNFVRLDPAVMPAHVFRIRRRTR
                     TERDFEYFM"
     gene            1564..>2841
                     /gene="treS"
                     /locus_tag="VEO00_00580"
     CDS             1564..>2841
                     /gene="treS"
                     /locus_tag="VEO00_00580"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HYU56530.1"
                     /translation="MRRATPPSLWFKDAIVYQTHVRAFSDSNADGIGDFRGLTARLDY
                     LADLGVTAIWLLPFYPSPLRDDGYDIADYTGVHHTYGTLRDFKAFLRAAHERDLRVIT
                     ELIINHTSDRHPWFQRARRARPGTPERDFYVWSDTPDRYPEARIIFKDFESSNWAWDR
                     VAGAYYWHRFYTHQPDLNFDSPAVRRAVARVVDFWLDLGVDGLRLDAVPYLFEREGTT
                     CENLEETHAFLRDLRRHVDANFADRMLLAEANQWPEDAAAYFGNGDECHMAFHFPLMP
                     RMFMAIRMEDRFPVIDILEQTPSIPETAQWALFLRNHDELTLEMVTDEERDYMYRAYA
                     SDPQARINLGIRRRLAPLLGNNRRRIELMNGLLFALPGTPVLYYGDEIGMGDNVFLGD
                     RNGVRTPMQWSPDRNAGFSRADPQQLYLPPIMDP"
BASE COUNT          458 a         1050 c          922 g          411 t
ORIGIN      
        1 gatcgccggc ctccgcggcg acggcggcgc cgcgacccgg ctggccgcgg ccaccgatcc
       61 cgccgtggcc gagctgctgg aggcgcaccc gcctcgctcc cgcacccgct cgcctcgcct
      121 gcggatccgg gtggaccggc cgcgggcgct ctacggcgcc tggtacgagc tgttcccccg
      181 ctccgagggg gcgaccgaga cctccagtgg cacgctgcgg caggcggccg agcggctgcc
      241 agcgatcgcc ggcatggggt tcgacgtggt gtacctgccg ccgatccacc cgatcggctt
      301 caccaaccgc aagggccgca acaacagcct cgccccgcga cgggacgacc cgggcagccc
      361 gtgggcgatc ggcggcgagg agggcggtca caccgccgtc catcccgacc tcggcacgct
      421 cgaggacttc cggcggctgg tggcgcgggc acgcgagctc ggcctcgaca tcgcgctcga
      481 cttcgcgatc cagtgctcgc ccgaccaccc gtggctgaag gagcacccgg agtggttcca
      541 ccaccgtccg gacgggacgc tcaagtacgc cgagaacccg cccaagcgct accaggacat
      601 ctacaacgtc aactgggaga ccgaggacag gcaagggctc tgggaggcga tccgcgacgt
      661 cgtcttccac tggatgagcc acggtgtcac ggtcttccgg gtcgacaacc cgcacacgaa
      721 gccgttcgcc ttctgggagt gggtcatcga cgacctgaag cgccgggcac cggagacgat
      781 cttcctgtcc gaggcgttca cccggccgcg cgtgctctac cggctggcca agctcgggtt
      841 cacgcagtcc tacacctact tcgcctggcg ccgcgaccgt gcggagctga ccgagtactt
      901 caccgagctc accgcgtcgg aggtgcggga gttctgccgc ccgaacctgt ggccgaacac
      961 ccccgacatc ctgaccgagg agctgcagca gggtggccgg gcggcgttcg tcgcgcggct
     1021 ggtcctggcc gcgaccctcg gcgcgagcta cggcatctac gggcccgtgt acgagctgtg
     1081 cgaggcccgc gccgtcaggc ccggatccga ggagtacctc gactcggaga agtaccagct
     1141 ccgccactgg gacctcgacc ggcccgactc gcttgccgac ctgatcgcgc gcgtgaaccg
     1201 gatccgccgc cagaacgccg cgctgcaggc cgactggagc ctccgcttcc acggcaccga
     1261 caacgaccgg ctgctctgct actcgaaggc gaccgcggac ggctcgaacg ccatcctggt
     1321 cgtggtcaac ctcgaccacc agcacccgca gtccggctgg accgcactct ggatggaagc
     1381 cctcggcctg gaggaggggg ccgagttcca ggtgcacgac ctgatcagcg aggggcggta
     1441 ccggtggcgc ggcccgcaca acttcgtccg gctggacccc gccgtgatgc cggcgcacgt
     1501 cttccggatc cgtcgccgta cccgcaccga acgagacttc gagtacttca tgtgaggacg
     1561 cccatgcggc gagcaacccc cccgtccctc tggttcaagg acgcgatcgt ctaccagacg
     1621 cacgtccggg ccttcagcga ctcgaacgcg gacgggatcg gggatttccg gggcctgacg
     1681 gcccggctgg actacctggc cgacctgggc gtcaccgcga tctggctgct gccgttctac
     1741 ccgtcgccgc tgcgcgacga cggatacgac atcgccgact acacgggcgt ccatcacacc
     1801 tacggcaccc tgcgagattt caaggctttc ctgcgggccg cccacgagcg ggaccttcgg
     1861 gtgatcaccg agctgatcat caaccacacg tccgatcgac acccctggtt ccagcgggcg
     1921 cgccgggccc gcccggggac gccggagcgc gacttctacg tctggagcga cacgcccgac
     1981 cgctaccccg aggcgcggat catcttcaag gacttcgaat cctcgaactg ggcgtgggat
     2041 cgcgtggccg gcgcgtacta ctggcaccgc ttctacaccc accagcccga cctgaacttc
     2101 gacagcccgg ccgttcgcag ggccgtcgcc agggtcgtcg acttctggct cgacctcggc
     2161 gtggacggcc tgcggctcga cgcggtcccg tacctgttcg aacgggaggg gaccacctgc
     2221 gagaacctcg aggagaccca cgcgttcctg cgcgacctgc ggcgccacgt cgacgccaac
     2281 ttcgccgacc gcatgctcct cgcggaggcc aaccagtggc cggaggacgc cgccgcctac
     2341 ttcggcaacg gcgacgagtg ccacatggcg ttccacttcc cgctgatgcc acggatgttc
     2401 atggcgatcc ggatggagga ccgcttcccg gtgatcgaca tcctggagca gacgccgtcg
     2461 atcccggaga cggcgcagtg ggcgctgttc ctgcgcaacc acgacgaact gaccctggag
     2521 atggtgaccg acgaggaacg cgactacatg taccgggcgt atgccagcga cccgcaggcc
     2581 cgcatcaacc tggggatcag gcgtcgcctc gcgccgctgc tcggcaacaa ccggcgtcgc
     2641 atcgagctga tgaacggcct cctgttcgcc ctgcccggta cgccggtcct gtactacggc
     2701 gatgagatcg ggatgggcga caacgtcttc ctgggcgatc gcaacggcgt gcgcacgccc
     2761 atgcaatgga gcccggaccg caacgcaggc ttttcgcgcg cagatccgca gcaactctac
     2821 ctcccgccga tcatggatcc g
//
LOCUS       DASPWO010000025         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_597265, whole genome shotgun sequence.
ACCESSION   DASPWO010000025 DASPWO010000000
VERSION     DASPWO010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597265"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VEO00_00585"
     CDS             <1..197
                     /locus_tag="VEO00_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56531.1"
                     /translation="APEPESPEAAAPETAEPEAPATETVAPETPAPEAPAPETVTPET
                     PAPETATSEGPAGETDEEKA"
     gene            198..617
                     /gene="rplP"
                     /locus_tag="VEO00_00590"
     CDS             198..617
                     /gene="rplP"
                     /locus_tag="VEO00_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HYU56532.1"
                     /translation="MLQPKKVKHRKQHRGRMRGQAKGGTDVHFGDFGLVALEPGWITN
                     RQIEAARVAMTRHIRRGGKVWINVFPDKPVTKKPAETRMGSGKGNPEWWVAVVKPGRV
                     MFELAGVTEPIAKEAMRRAAQKLPVRTRFVTRLGAVH"
     gene            617..811
                     /gene="rpmC"
                     /locus_tag="VEO00_00595"
                     /pseudo
     CDS             617..811
                     /gene="rpmC"
                     /locus_tag="VEO00_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041323024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
     gene            1069..1341
                     /gene="rpsQ"
                     /locus_tag="VEO00_00600"
     CDS             1069..1341
                     /gene="rpsQ"
                     /locus_tag="VEO00_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007469125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HYU56533.1"
                     /translation="MPTDEGGARQRRKVRVGVVVSDRMDRTVVVRVSDAVTHPMYGKT
                     VRHTSKLAADDPQNEAHVGDTVRVMETRPLSKTKRWRVTEIVERAK"
     gene            1356..1724
                     /gene="rplN"
                     /locus_tag="VEO00_00605"
     CDS             1356..1724
                     /gene="rplN"
                     /locus_tag="VEO00_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HYU56534.1"
                     /translation="MIQQETRCKVADNTGAKEVLCIRVLGGSSRRYAGVGDVIVATVK
                     DALPGGGVKKGDVVRAVVVRTAKERRRGDGSYIRFDDNAVVLINDQRNPRGTRIFGPV
                     ARELREKRFMKIISLAPEVL"
     gene            1727..2041
                     /gene="rplX"
                     /locus_tag="VEO00_00610"
     CDS             1727..2041
                     /gene="rplX"
                     /locus_tag="VEO00_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HYU56535.1"
                     /translation="MATVSIKKGDEIMVMAGKDRGRSGRVVNVLPARGRIMVEGVARA
                     KKATRPSRKRQQGGIIDIEQFIDISNVQLLCPSCHKPTRVGYRFEGTDKIRICRKCGA
                     DI"
     gene            2041..>2329
                     /gene="rplE"
                     /locus_tag="VEO00_00615"
     CDS             2041..>2329
                     /gene="rplE"
                     /locus_tag="VEO00_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HYU56536.1"
                     /translation="MAQAPAYPAPRLKERYRGEIRPQLRSEHGYRGLMEVPRLEKIVV
                     NMGVGDAVRDAKLLDAAVLDLATVTGQKPMITRARKSIAGFKLREGMAIGAK"
BASE COUNT          403 a          775 c          882 g          269 t
ORIGIN      
        1 ccgcgcccga gcccgagtcg cccgaagcag ccgcgcccga gacggccgag cccgaggcgc
       61 ccgcgaccga aacggtcgcc cccgagacgc ccgcgcccga ggcgcccgcg cccgaaacgg
      121 tcacccccga gacgcccgcg cccgagacgg ccacgtccga gggcccggcg ggcgagacgg
      181 acgaggagaa ggcataagtg ctccagccga agaaggtcaa gcaccgcaag cagcatcgcg
      241 gtcgcatgcg cggacaggcg aagggcggga ccgatgttca cttcggcgac ttcggcctgg
      301 tcgccctgga gcccggttgg atcacgaacc gccagatcga ggcggcccgg gtggccatga
      361 cccgtcacat ccgccggggc gggaaggtgt ggatcaacgt cttccccgac aagccggtga
      421 ccaagaagcc cgccgagacc cggatgggtt ccgggaaggg caatccggag tggtgggtgg
      481 cggtggtcaa gcccggccgg gtgatgttcg agctggccgg cgtgaccgag ccgatcgcca
      541 aggaggccat gcgccgcgcg gcccagaagc tgccggtgcg cacccggttc gtcacccgac
      601 tgggggcggt gcactgatgg cgcggcgcgt gacggtcgag ctccgggagc tgccggacga
      661 cgagctgctc acccggctgg agggcggcaa ggaggagctg ttcaacctgc ggttccagct
      721 ggccaccggg cagctcgaca acccgatgcg gatcaagcag gtccgccacg agctcgcccg
      781 gatcctgacc atcctgcgcg agcgccacct ggaggcagag ctcgagcatc agctggccct
      841 ggccgagtcc gaggccctgc agcgcgggcg cgaggaacag gctcgcggcg agcgcaaggg
      901 cacgccgctc gccgaggcgg tcggcgagat gcccgagggc gacgagccgg cgatggccat
      961 gggccgcgac gcggcggagg gcgaggcctc gagcgagccc cccgaggaga ccgagaggcc
     1021 tcgccggcgg ttccgccgcc gccagaagga ggacgacgag tgagcgagct gcccacggac
     1081 gagggtggag cgcggcagcg gcgcaaggtg cgggtcggcg tcgtggtgtc cgaccgcatg
     1141 gaccgcacgg tggtcgtgcg ggtctcggac gccgtgaccc acccgatgta cggcaagacg
     1201 gtccgccaca cgtcgaagct cgccgccgac gacccgcaga acgaggcgca tgtgggagac
     1261 accgttcggg tcatggagac ccggccgctg tcgaagacca agcgctggcg cgtcaccgag
     1321 atcgtcgaac gcgccaagta aggacggagc ggaacgtgat ccagcaggag acccgctgca
     1381 aggtggcgga caacacgggg gccaaggagg tcctctgcat ccgcgtcctc ggcgggtcgt
     1441 ctcggcgcta cgccggcgtc ggcgacgtca tcgtggcgac ggtgaaggac gccctccccg
     1501 gcggcggcgt caagaagggc gacgtcgtcc gggccgtggt cgtgcgcacc gcgaaggagc
     1561 gccgccgcgg cgatgggtcc tatatccgct tcgacgacaa cgcggtggtg ctgatcaacg
     1621 accagcgaaa cccgcgcggg acgcgcatct tcggcccggt ggcgcgtgag ctccgggaga
     1681 agcggttcat gaagatcatc tctctcgcgc cggaggtgct gtagcggtgg cgacggtatc
     1741 gatcaagaag ggcgacgaga tcatggtgat ggccggcaag gaccgcggcc ggtccgggcg
     1801 ggtcgtcaac gtgctgccgg ccaggggccg gatcatggtc gagggcgtgg cgcgcgccaa
     1861 gaaggccacg cgcccgtcgc gcaagcgcca gcagggcggg atcatcgaca tcgagcagtt
     1921 catcgacatc tcgaacgtgc agctcctgtg tccctcgtgc cacaagccga cgcgggtcgg
     1981 gtaccggttc gagggcaccg acaagatccg gatctgccgc aagtgcgggg cggacatctg
     2041 atggcgcagg cacccgccta cccggccccg cggctcaagg agcgctaccg gggtgagatc
     2101 cggccgcagc tgcgctcgga gcacggctat cgcggcctga tggaggtccc gcgcctcgag
     2161 aagatcgtgg tgaacatggg cgtcggtgac gccgtgcgcg acgcgaagct gctcgacgcg
     2221 gccgtcctcg acctggccac cgtgaccggc cagaagccga tgatcacccg ggcccgcaag
     2281 tcgatcgccg gcttcaagct ccgggagggg atggcgatcg gcgcgaagg
//
LOCUS       DASPWO010000026         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1106302, whole genome shotgun sequence.
ACCESSION   DASPWO010000026 DASPWO010000000
VERSION     DASPWO010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106302"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..664
                     /locus_tag="VEO00_00620"
     CDS             338..664
                     /locus_tag="VEO00_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HYU56537.1"
                     /translation="MSRCRWSASGAATEWGATAKVIVGMTMSIDGFVADRSGDVGRLY
                     PDLAALRGTPYMDAAVAETGAVLMGRRTFEMGDPDSYVGTYEFQVPIFVVTTGRHRSP
                     RSRTST"
     gene            700..1137
                     /locus_tag="VEO00_00625"
     CDS             700..1137
                     /locus_tag="VEO00_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HYU56538.1"
                     /translation="MAQATAAAGDGAVQVVGGPTLIRQLLDAGLADELRIDVMPVLLG
                     SSLRLFDDGGRERLEVEKSGGRGDRAEDEPQVPPPLNLPLDAGSSVRYFTDQLFNWEV
                     EWRRRPINWTGRSRRWRIPRGGRSWPAWRGARPRSRSSPSPST"
     gene            1029..1370
                     /locus_tag="VEO00_00630"
     CDS             1029..1370
                     /locus_tag="VEO00_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYU56539.1"
                     /translation="MDRTFAALADPTRRAILARLARGEASVTELAEPFDMTLPAVSKH
                     LKVLERAGLVARGRERQWRPARLQAEPLKEVADWAERYRRFWEESYDRLDEYLEKIQA
                     GGKEDGDGRRR"
     gene            1354..1848
                     /locus_tag="VEO00_00635"
     CDS             1354..1848
                     /locus_tag="VEO00_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYU56540.1"
                     /translation="MAGDGSKLTVTTPSDREIVMTRVFDAPRDLVFEAHTSCEHMRHW
                     WGPRRYEIADCEIDFREGGAWRVVHRAPGEEHVFSGEFREIVRPERITWTFEWGGAPG
                     HGSVDTLTLEEHDGKTTLTTTSVFSAVEDRDGMLESGASEGAAETWDRLAEYLEVLKK
                     GAPG"
     gene            complement(1876..>2208)
                     /locus_tag="VEO00_00640"
     CDS             complement(1876..>2208)
                     /locus_tag="VEO00_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56541.1"
                     /translation="LDLLGLPPMATDGVSRLPELWSGRPIPLHDVVAVDGNATRVAIT
                     RTPYKLVRTRAWAVLDGSFEPPGTSRLYRTDRPDEAVTEDPIAAELDRAVDAWMRAHG
                     LPATMGLP"
BASE COUNT          332 a          724 c          837 g          315 t
ORIGIN      
        1 ctaggcgggg cgatccactc ggtgggattc ggccctgctc gactctgatg gtgcgatcga
       61 ccccggcttc cggcggcctc tgaagcggct ccatccccag gcgcccaggg cgagaagcac
      121 gacggcgacg atggcgacga acacccagta gagcggggag aagccaccac ctccacacct
      181 ccgccctggt ctccgccgcc gtactgggcg acgatcgtcc acagcgctcc tacggtcatt
      241 ggtgacacct cctccgcaag tctccccacc ggccactggc ccaacccgca acggcggagg
      301 cgcggatgga cgcgtcgggt gaatagggtg ggtcgcggtg agtaggtgcc ggtggtcggc
      361 gtccggcgcc gcgaccgaat ggggcgccac ggcgaaggtg atcgtcggga tgacgatgtc
      421 gatcgacggg ttcgtcgccg atcggagcgg cgacgtgggc cgtctctatc ccgacctcgc
      481 cgccctgcgg ggcaccccgt acatggacgc ggcggtcgcg gagaccgggg cggtgctcat
      541 ggggcggcgg accttcgaga tgggggatcc cgactcctac gtcgggacct acgagttcca
      601 ggtgccgatc ttcgtggtga ccaccggccg ccatcggtcg ccccgaagca ggacgagcac
      661 ttgacgttca cgttcgttac cgagggtgtc caggcggcgg tggcgcaggc aaccgcggcc
      721 gccggggacg gggccgtcca ggtcgtcggc ggccccacgt tgatccgcca gctcctcgac
      781 gccggactgg ccgacgagct tcggatcgac gtgatgccgg tgctgctcgg ctccagcctg
      841 cgcctgttcg acgacggcgg gagggaacgt ctcgaggtgg agaagtccgg cggtcgagga
      901 gatcgggccg aggacgagcc tcaggttccg cctccgctga acttgcccct tgacgccggt
      961 tcgtcggtgc ggtattttac cgatcagtta tttaactggg aggttgaatg gcgacggcga
     1021 ccgatcaact ggaccggacg ttcgcggcgc tggcggatcc cacgcggcgg gcgatcctgg
     1081 cccgcctggc gcggggcgag gcctcggtca cggagctcgc cgagcccttc gacatgacgc
     1141 tgcccgcggt gtcgaagcat ctgaaggtgc tcgagcgggc cggactcgtc gcgcgagggc
     1201 gggaacggca gtggcggccg gcccggctgc aggccgagcc gctgaaggag gtcgccgact
     1261 gggccgagcg gtaccgccgg ttctgggagg agagctacga ccgtctggac gagtacctgg
     1321 agaagatcca ggccggaggg aaggaggacg gcgatggccg gagacggtag caagctcacg
     1381 gtgacgaccc cgtcggaccg ggagatcgtc atgacccgcg tgttcgacgc gccccgcgac
     1441 ctcgtgttcg aggctcacac ctcgtgcgag cacatgcggc actggtgggg gccgcgaagg
     1501 tacgagatcg ccgactgcga gatcgacttc cgcgagggcg gcgcctggcg ggtcgtgcat
     1561 cgggctcccg gcgaggagca cgtcttcagc ggtgagttcc gcgagatcgt ccggccggag
     1621 cggatcactt ggacgttcga gtggggcggc gcgccgggcc acgggtcggt cgacacgctc
     1681 accctcgagg agcacgacgg gaagacgacg ctcacgacca cgtccgtgtt cagcgcggtg
     1741 gaggatcgcg acgggatgct cgagtccggc gcctcggagg gcgccgccga gacctgggac
     1801 cgcctcgccg agtacctcga ggtcctcaag aagggcgcgc cgggctgacc gagagccgcc
     1861 cgcaagtcgc gagggctacg ggagtcccat cgtcgccggg aggccgtggg cgcgcatcca
     1921 ggcgtcgacc gcgcggtcga gctcggcggc gatgggatcc tccgtcaccg cctcatccgg
     1981 gcggtcggtc cggtagagcc ggctggtgcc cggcggctcg aaagagccgt cgaggaccgc
     2041 ccaggcccgc gtcctgacca gcttgtaggg cgtccgggtg atggcgaccc gggtggcgtt
     2101 cccgtcgacg gcgaccacgt cgtggagcgg gatgggccgg ccactccaga gctccgggag
     2161 ccggctcacg ccgtccgtcg ccatcggggg caggccgagc aggtcgag
//
LOCUS       DASPWO010000027         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_3434, whole genome shotgun sequence.
ACCESSION   DASPWO010000027 DASPWO010000000
VERSION     DASPWO010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3434"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VEO00_00645"
     CDS             <1..423
                     /locus_tag="VEO00_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYU56542.1"
                     /translation="PRAVFVLAAGLAFALSAQTAALAATVDVTVADFAFSPKGVKVPQ
                     GTVVRWTNDGPHSHTSTSDSTNPDGSKGIALWNSGVIVVGGYPYSRTFTAAGGFPYHC
                     NIHPTMNGSVTVPVKGPKGGVVGQSFTVGGACSRRRSR"
     gene            629..1537
                     /locus_tag="VEO00_00650"
     CDS             629..1537
                     /locus_tag="VEO00_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HYU56543.1"
                     /translation="MEADRPSGRDTALIERFLEHLADGRHLSPNTVAAYRGDLEGLAT
                     FLARGGRSIDDADHPILRRWLAHLATRGYARASMARKAAAVRSLYRYLVRQGLVEADP
                     AAALVTPKLGSRLPVVLKPEEAGRVVEAPAGLEPWVFRDRAILELLYGCGLRVAELCG
                     LDVDDVDLDRGRVRVLGKGGKERELPLGDQAADALEAYLREARGAMGMPGERALLFNR
                     RAKRLSTRDARSVVARYRHAIGADRQMSPHTLRHSFATHLLEGGADIRAVQELLGHAS
                     LATTQRYTHVSRGRLFGAYRLSHPRA"
     gene            1537..2334
                     /gene="whiG"
                     /locus_tag="VEO00_00655"
     CDS             1537..2334
                     /gene="whiG"
                     /locus_tag="VEO00_00655"
                     /inference="COORDINATES: protein motif:HMM:NF004935.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HYU56544.1"
                     /translation="MASSTSAETPTLLDSLWDEFKRTGSATAREQLILHYAPLVKYVA
                     GRIATGLPSSVEQADLASYGMFGLIDALDKFDLARSIKFETYAIPRIKGAILDGMRSV
                     DWVPRSVRFKAREVEKAIADLEASLKRSPTDAEIAVHLRISRGELHDVMSQISFVSVM
                     ALDEAVGGTGDRGEGISLIDTLPDRSSLDAMGAIEGQEGRGMLAAAINSLSEREKIVV
                     TLYYFEGLTLNEIGQVLGVTESRICQIHTKAVGSLRRQMIEVDDGAV"
     gene            2419..>2716
                     /locus_tag="VEO00_00660"
     CDS             2419..>2716
                     /locus_tag="VEO00_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HYU56545.1"
                     /translation="MRAPNRTIFLVLLVAAWLLTPWPGSAGAAPVPGSYAWPVRGPVI
                     RPFEAPASPYGPGHRGIDIAATPDTVVGAAEGGVVAFAGVVAGARYVSIDHPDGV"
BASE COUNT          363 a          965 c          992 g          396 t
ORIGIN      
        1 cctcgcgccg tgttcgtgct cgctgccggc ctggccttcg cgctctccgc gcagaccgcg
       61 gcgctggccg ccacggtcga cgtgacggtg gccgacttcg cgttcagccc gaagggcgtc
      121 aaggtgccgc aggggacggt cgtccgctgg acgaacgacg ggccgcactc gcacacctcg
      181 acgagcgaca gcacgaaccc ggacggctcc aaggggatcg ccctgtggaa ctccggcgtg
      241 atcgtcgtgg gcggctaccc gtactcgcgc accttcacgg cggcgggcgg gttcccctac
      301 cactgcaaca tccacccgac catgaatggg tccgtcacgg tgccggtcaa gggaccgaag
      361 ggcggcgtgg tcgggcagtc gttcacggtg ggcggagcct gttctcgccg acgaagtcgg
      421 tgatggtcgc cgcctgaagg gcgtcgcggg ccgtgcggcg agcggcaact cgcggcgcgg
      481 cccgttccgc cgaccccttg gggcgcggga ggtccggtgg ggccggtgac gcctccctcc
      541 cgatgggggc cgatccgagt gcttgcaccg cgggcgccgg tgctccaagg ttgctccgca
      601 cttccccgac cccctgagga gccgagccgt ggaggccgac cgcccgtccg ggcgtgatac
      661 cgccctgatc gagcggttct tggagcacct ggccgacggg cgccacctgt cgccgaacac
      721 ggtggcggcc taccgcggcg acctcgaggg cctggccacg ttcctggccc ggggcggccg
      781 atcgatcgac gacgccgacc accccatcct gcgccgctgg ctcgcccacc tcgccacccg
      841 cgggtacgcc cgcgcctcga tggcgcgcaa ggctgcggcc gttcgatccc tctaccggta
      901 cctggtccgt cagggcctgg tcgaggccga cccggccgcc gcgctcgtca ccccgaagct
      961 cgggtcgcgg ctgcccgtcg tgctgaagcc ggaggaggcc gggcgcgtgg tcgaggcgcc
     1021 ggccggcctg gagccgtggg tgttccggga ccgggcgatc ctcgagctgc tgtacggctg
     1081 cgggctgcgc gtcgcggagt tgtgcgggct cgatgtcgac gacgtggacc tcgaccgtgg
     1141 ccgggtccgg gttctcggca agggcggcaa ggagcgcgag ctgccgctcg gcgaccaggc
     1201 cgccgacgcg ctggaggcat acctgcgtga ggcccgcggg gcgatgggaa tgcccgggga
     1261 acgggcgctg ctcttcaatc gccgcgcgaa gcggctgtcc acgcgcgacg cgcggagcgt
     1321 ggtggcaaga taccggcacg ccatcggcgc cgatcgccag atgagtccgc acacgcttcg
     1381 ccactcgttc gccacgcacc tgctcgaggg aggggccgac atccgagccg tccaggagct
     1441 cctgggacac gccagcctcg ccaccaccca gcggtacacg cacgtctcgc gcggcaggct
     1501 gttcggggcg taccgcctga gccacccgag ggcctgatgg cgtcgtcgac ctcggcggag
     1561 accccgacgc tcctcgactc gctgtgggac gagttcaagc gaacggggtc cgcgacggcg
     1621 cgagagcagc tgatcctgca ctacgccccg ctcgtgaagt acgtcgccgg ccggatcgcc
     1681 accgggctgc cgtcttcggt ggagcaggcc gacctggcga gctacgggat gttcggcctg
     1741 atcgacgcgc tggacaagtt cgacctcgcc cgctcgatca agttcgagac ctacgccatc
     1801 ccgcggatca agggcgccat cctcgacggg atgcgctcgg tggactgggt cccgcgctcg
     1861 gtgcgcttca aggcccgcga ggtcgagaag gcgatcgccg acctggaggc cagtctgaag
     1921 cggtcaccga ccgacgcgga gatcgccgtg cacctgcgga tcagccgggg ggaactgcac
     1981 gacgtgatga gccagatctc gttcgtctcg gtgatggcgc tcgacgaggc cgtcggcggc
     2041 accggcgacc ggggcgaggg aatctccctg atcgacaccc tgccagaccg ttcgtcgctc
     2101 gacgccatgg gggcgatcga ggggcaggag ggccgcggca tgctggccgc cgcgatcaac
     2161 tcgctgtcgg agcgggagaa gatcgtcgtc acgctctact acttcgaggg tctgaccttg
     2221 aacgagatcg gccaggtgct cggcgtgacc gagtcgcgga tctgccagat ccacacgaag
     2281 gccgtcggct cgcttcgcag gcagatgatc gaggtcgacg acggcgccgt ttgacggctg
     2341 cggtgtgaac gcggtcactg acaccggcag ccggttcggg taacgtcctc ggcgcctccg
     2401 gggtgcgagc gaggtgacgt gcgcgcaccc aaccggacca tcttcctcgt gctgctcgtc
     2461 gcggcgtggc tgctgacgcc gtggccgggg tccgccggcg cggcgccggt ccccggttcc
     2521 tatgcctggc cggttcgcgg tccggtgatc cggccgttcg aggcgccggc ctccccctac
     2581 ggccccgggc accgcgggat cgacatcgcc gccacgcccg acaccgtcgt cggggccgcg
     2641 gagggcggcg tggtggcctt cgccggcgtc gtggctggcg cgcgctacgt ctcgatcgac
     2701 caccccgacg gcgtgc
//
LOCUS       DASPWO010000028         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_597732, whole genome shotgun sequence.
ACCESSION   DASPWO010000028 DASPWO010000000
VERSION     DASPWO010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597732"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VEO00_00665"
     CDS             complement(<1..516)
                     /locus_tag="VEO00_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HYU56546.1"
                     /translation="MPRPTRRHRLAAFVAAVATLLVLTVVLAPGPPPGPRRDLAAVGS
                     RYHVKVLRDTWGVPHVFGQSDADTAYGLAWAHAEDDFATIQRQLLGARGRLASVLGRE
                     GAASDYVVALLRVRATVDARYATDLSRETRAVVEAYADGINHYAATHRREALPGLYPV
                     EGKDVVAGFVAG"
     gene            593..1801
                     /locus_tag="VEO00_00670"
     CDS             593..1801
                     /locus_tag="VEO00_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU56547.1"
                     /translation="MDFAIRPEIQELREKVLRFVERSLYPKEAAWLRWEEEHFPACIE
                     DHEEIRALQREARAMGVWAGHLPKEAGGMGLSVADYGLLNEIIGRAYHAPRVFGSNAP
                     DSGNAEILWHFGTPAQKERFFWPLQRAEVRSFFGMTEPEVSGADPTSLQTTAVRDGGH
                     WVVNGHKWYSSNANGAAFGIVMAATEPGAPAHRRYSQIIVPADTPGLELVRPISVMGH
                     RTGPGHWEVRLRDARVPVENVLGEPGQGFAIAQARLGPGRIHHCMRWLGQAQRGFELM
                     CTYARRRQAFGGPLSDKQTVQNWIADSAAEIQAARLMTLHAAWTIDREGAKAARVDIS
                     LIKFFGAKVLLDVLDRAIQVHGGLGVSDDTPLAFMWRQARSARIYDGPDEVHKMLVAR
                     EVLKAFPEKA"
     gene            1803..>2393
                     /locus_tag="VEO00_00675"
     CDS             1803..>2393
                     /locus_tag="VEO00_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HYU56548.1"
                     /translation="MPPDLPGDTIEVRADERLDEARLADYLRGRLPGAEGPLTVRQFG
                     GGAANLTYLLQYGPSEYVLRRPPLGPVAPSSHDMGREYRVLSVLHRAFPPAPRAFHLC
                     EDASVIGAPFFVMERRLGVVVRREVPAAFAAIPAAPRRMGLALVDALADLHAVDYAGL
                     GLETLGKPAGFLERQIEGWLGRWEKATLVDVPDMTWV"
BASE COUNT          335 a          864 c          876 g          318 t
ORIGIN      
        1 gccggccacg aaccccgcca ccacgtcctt gccctccacg ggatagaggc cgggcagcgc
       61 ctcccgccga tgcgtggctg cgtagtggtt gatcccgtcg gcgtaggcct cgacaaccgc
      121 ccgcgtttcc cgggagagat cggtcgcgta gcgggcgtcg accgtcgccc ggactcgcag
      181 cagggccacg acgtaatccg aggccgctcc ctcgcggccc agcaccgagg ccagcctgcc
      241 gcgagcgccg aggagctgcc gctggatggt ggcgaaatcg tcctccgcgt gggcccaggc
      301 gaggccgtaa gccgtgtcgg cgtcgctctg accgaagacg tgcggcacgc cccaggtgtc
      361 gcgcagcacc ttcacgtggt agcgggagcc caccgcggcg aggtcccggc gaggaccggg
      421 cggcgggccg ggagcgagca cgacggtgag gacgagcagg gtggcgacgg ccgccacgaa
      481 agcggccagg cggtggcggc gggtgggacg aggcattcgg cgggggagtt tactccagcc
      541 gatccgctcg gcgatactcg acgggccgcg ccgactcgtg ggaggagccc ccgtggactt
      601 cgcgatccgc ccggagatcc aggagctcag ggagaaggtc ctgcgcttcg tcgaaaggag
      661 cctctacccg aaggaggccg cgtggctgcg ctgggaggag gagcacttcc cggcctgcat
      721 cgaggaccac gaggagatcc gcgccctcca gcgcgaggcg cgggccatgg gcgtctgggc
      781 gggccacctc ccgaaggagg ccggggggat gggcctgtcg gtggcggatt acgggctgct
      841 caacgagatc atcggccgcg cctaccacgc gccgcgcgtc ttcggctcga acgcccccga
      901 cagcgggaac gccgagatcc tgtggcactt cggcaccccg gcccagaagg agcgcttctt
      961 ctggccgctc cagcgggccg aggtgcggtc gttcttcggg atgaccgagc ccgaggtgag
     1021 cggagccgac cccacgtcgc tgcagaccac cgccgtgcgg gacggcgggc actgggtggt
     1081 caacggccac aagtggtaca gctcgaacgc caacggcgcg gccttcggaa tcgtcatggc
     1141 cgccaccgag cccggcgcgc ccgcgcatcg ccgctacagc cagatcatcg tccccgccga
     1201 cactcccggc ctcgagctcg tgcggccgat ctcggtgatg ggccaccgca ccggccccgg
     1261 ccactgggag gtgcgcttgc gggacgcccg ggtgccggtc gagaacgtgc tcggcgagcc
     1321 cgggcagggc ttcgcgatcg cccaggcccg cctcggtccc ggccgcatcc accactgcat
     1381 gcgctggctc ggccaggccc agcgcggctt cgagctgatg tgcacctacg cgcggcggcg
     1441 ccaggccttc gggggcccgc tcagcgacaa gcagaccgtg cagaactgga tcgccgacag
     1501 cgcggccgag atccaggccg cccggctcat gaccctgcac gcggcgtgga ccatcgaccg
     1561 ggagggcgcc aaggcggccc gggtcgacat ctcgctgatc aagttcttcg gggcgaaggt
     1621 gctcctggac gtgctcgacc gggccatcca ggtccacggc gggctcgggg tctccgacga
     1681 caccccgctc gccttcatgt ggcgccaggc ccggagcgcc cgcatctacg acggccccga
     1741 cgaggtccac aagatgctgg tcgcgcggga ggtgctgaag gccttcccgg agaaggcctg
     1801 aaatgccgcc ggacctgccg ggcgacacca tcgaggtgcg cgccgacgag cgtctcgacg
     1861 aggcacgcct cgccgactac ctgcgcggcc ggctcccggg ggccgagggc ccgctcacgg
     1921 tccgccagtt cgggggcggg gcggccaacc tgacctatct tcttcaatac ggcccgagcg
     1981 agtacgtgct gcgccggccg cccctggggc cggtcgcacc ctcctcccac gacatgggcc
     2041 gcgagtaccg cgtgctgtcc gtcctccacc gggccttccc gcccgctccg cgcgccttcc
     2101 atctctgcga ggacgcgagc gtgatcgggg cgccgttctt cgtgatggag cggcgtctcg
     2161 gcgtcgtggt ccggcgggag gttccggccg cgttcgccgc catcccggcc gcgccgcggc
     2221 ggatgggcct cgctctcgtg gacgccctgg ccgacctgca cgcggtcgac tatgcgggcc
     2281 tcggcctgga gacgctcggc aagccggccg gcttcctgga gcggcagatc gagggctggc
     2341 tcggccgttg ggagaaggcg acgctcgtgg acgtccccga tatgacgtgg gtt
//
LOCUS       DASPWO010000029         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_514603, whole genome shotgun sequence.
ACCESSION   DASPWO010000029 DASPWO010000000
VERSION     DASPWO010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514603"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..440)
                     /locus_tag="VEO00_00680"
     CDS             complement(156..440)
                     /locus_tag="VEO00_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56549.1"
                     /translation="MSVLRIEHPVRDFEAWKQAFDRDPVGRERSGVRRYRVLRPVDDP
                     NYVLVDLEFDTAREAEALLTAMRAVWDRVQGTIMSEPQARIVEAVETGEY"
     gene            526..1197
                     /locus_tag="VEO00_00685"
     CDS             526..1197
                     /locus_tag="VEO00_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYU56550.1"
                     /translation="MTRAVKRRRSYDSPRRREQARATRRAILGTARELFVERGYVATT
                     IEAIAARASVSPETVYAAFGNKRAVLSELIDVSIVGDDAPVPVLDRSWVLDMRDEREP
                     RRRVAILARNGRLILERLAPIYEVLRSAADADPPIAALWKRYKVQRHAGQHELVRIVA
                     VGGALRPGLTLKSADDILFAVGSPETYGLLVHDCGWSPRRFERWYADTLARLLLGLAV
                     STTPR"
     gene            1216..1521
                     /locus_tag="VEO00_00690"
     CDS             1216..1521
                     /locus_tag="VEO00_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HYU56551.1"
                     /translation="MSHQPERAAVPGYYANKVDLQARLKRIEGQVRGVERLIDEDRYC
                     IDILTQISAINAGLRKVAVKLLEDHIGHCIAETIAAGGDPRDKVLEASAAIDRILKA"
     gene            1611..>2056
                     /locus_tag="VEO00_00695"
     CDS             1611..>2056
                     /locus_tag="VEO00_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HYU56552.1"
                     /translation="MEHGAMHGREPTQHPEATDHAGHGGGHHDGGHGHGGHGDHAAMF
                     RDRFWLTLALTIPVVLYSEMFQRLLDYTAPRFPGSGWVSPVLGSVIFLYGGWPFLAGA
                     VDELRRRQPGMMLLIGLAITVAFAASWATEAGAVDLDFWWELAALIA"
BASE COUNT          334 a          713 c          716 g          293 t
ORIGIN      
        1 ggtcgagaat ccgcacctcc acgagctggt cgatccgctc ggccccccgc tcctggacga
       61 cctgctcggc cggctgcgtc acggatctcc cgaagcgcaa gccggccgga gggaaccaca
      121 ggaaccgtcg aaggcggccc cgaatcggga gcgcgtcagt actcgccggt ctcgacggcc
      181 tcgacgatcc gcgcctgcgg ctcggacatg atcgtgccct gcacccggtc ccagacggcg
      241 cgcatggcgg tgagcagcgc ctcggcctcc cgggccgtgt cgaactcgag gtcgaccagg
      301 acgtagttcg ggtcatccac gggccgcagg acgcggtagc ggcggacgcc ggaccgctcc
      361 cggccgacgg gatcgcggtc gaaggcctgc ttccaggcct cgaagtcgcg gacgggatgc
      421 tcgatccgca ggacggacat cggctcctcc tctctcggac ggcttcgccg ccacgaccat
      481 ttcatcgcag taggactgta tccgatacag tggtactgta gcagaatgac gagggccgtc
      541 aagcggcggc gttcctacga ctcaccccgg cgccgggagc aggctcgcgc cacccggagg
      601 gcgatcctcg ggaccgcccg ggagctgttc gtcgagcgcg gctacgtcgc gacgacgatc
      661 gaggcgatcg ctgcccgcgc gagcgtctcg cccgagacgg tgtacgcggc gttcgggaac
      721 aagcgagcgg tgctctcgga gctcatcgac gtgtcgatcg tgggggacga tgcgccggtg
      781 ccggttctcg atcgctcctg ggtcctcgac atgcgggacg agcgggagcc gcggcgccgg
      841 gtggccatcc tcgccaggaa cgggaggctg atcctcgagc ggcttgcccc gatctacgag
      901 gtgcttcgca gcgccgctga cgccgacccg ccaatcgccg cgctgtggaa gcggtacaag
      961 gtgcagcgcc acgccggcca gcacgagctg gtccggatcg tggccgtagg cggagccctc
     1021 cgccccgggc tcacgctgaa gtcggccgac gacatcctct tcgcggtcgg gagcccggag
     1081 acctacgggc tgctcgtcca cgattgtggc tggtcaccgc gtcgcttcga gcgctggtac
     1141 gcggacaccc tcgcccggct gctgctggga cttgcggtga gtaccacacc ccggtagggt
     1201 atagcctaaa gaaaaatgag ccaccagcca gagcgagctg cggtacccgg gtactatgcg
     1261 aacaaggtcg acctccaggc gcgcctgaag cggatcgagg gacaggtccg cggcgtcgag
     1321 cgtctgatcg acgaggatcg gtactgtatc gacatcctca cgcagatctc ggcaatcaac
     1381 gcagggctcc ggaaggtcgc cgtcaagctc ctcgaggacc acatcggcca ctgcatcgcg
     1441 gagacgatcg ccgcgggcgg tgatccccgc gacaaggtcc tcgaggcgag cgccgcgatc
     1501 gaccgcatcc tgaaggcgtg aggaaggacg tgtccatgga cccgcacgag caccacggag
     1561 cccatcgcgc cgtgaaggag acgggagccc ccgggccgca cgacaccacg atggagcacg
     1621 gtgcgatgca cggccgcgaa ccgacgcagc atccggaggc gaccgatcac gccggccacg
     1681 ggggcggcca tcacgacggc ggccacggcc acgggggaca cggcgaccac gccgcgatgt
     1741 tccgcgaccg cttctggctg acgctcgcgc tcacgatccc ggtcgtcctg tacagcgaga
     1801 tgttccaacg gctcctggac tacacggcgc cgagattccc cgggagcggc tgggtgtcgc
     1861 cggtgctggg aagcgtgatc ttcctctatg gcggctggcc cttcctggcg ggcgccgtcg
     1921 acgagctccg ccgccggcag cccgggatga tgctcctgat cggcctcgcc atcacggtgg
     1981 cgttcgcggc gagctgggca accgaggccg gagcggtcga cctcgacttc tggtgggagc
     2041 tcgccgccct gatcgc
//
LOCUS       DASPWO010000030         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_597752, whole genome shotgun sequence.
ACCESSION   DASPWO010000030 DASPWO010000000
VERSION     DASPWO010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597752"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..896)
                     /locus_tag="VEO00_00700"
     CDS             complement(162..896)
                     /locus_tag="VEO00_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYU56553.1"
                     /translation="MSTHPVLVNTGHGLIGAIITEPDGNPRRAAAILFEGREGRRSGV
                     NQVWTLTARELAGMGVTVLRLDYPGWGDSWAVPPGIPPGLPMREVIAWFRERVGDVDL
                     LFIGACFGSRLAAAVATEVPNVVGLALVNPYVRIPPSKKFTARARRAVTRLAGRSPRE
                     RFDRSLKDVFRGAFRTVPSLAIVADGDGRTPAFMTMQRTLREEGVPLEVQTFSGAGNR
                     TLGGQREGRERVVAWARSRLHELSPR"
     gene            complement(893..1669)
                     /locus_tag="VEO00_00705"
     CDS             complement(893..1669)
                     /locus_tag="VEO00_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56554.1"
                     /translation="MQEWASASTWTGDGLRREVTFFPSGRDEIYASLYAAEPPRLPVG
                     VVVCPSWGHDVMEQLELTHRLAHAIAARGGAGLAYHPPGHADSGGRFEEATFERLAEA
                     ARDALALAVSSVPEVAWEFGGVDLGAAVAVLAAARNGRILPLVEPALDPAAHLGVLER
                     AARRAALGRVDPEPTVFGYPLTRRIRSSAAALDVAGALESFGGRVAAFRHRLPERPPL
                     PERVETHVVPGEWRRPVHATNVEALVTGFGHWVAAAVAAA"
     gene            1784..3283
                     /locus_tag="VEO00_00710"
     CDS             1784..3283
                     /locus_tag="VEO00_00710"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF009005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HYU56555.1"
                     /translation="MPDVVVRDLLTPDPVTVRPADPIGVAANAMRARGVGSALVTDDG
                     RLVGIITERDMVLSTAGGARPDTSTVAAWMTPDPVTVSAGTSADVALDLLSQKGFRHL
                     PVVDDGKLAGIVSLRDLVAGLRVTRVDPTRPSTAKGLENVVVGETRKSFIDGERGRLV
                     YVGRDAVELALNHPFEAVWFLLMEERLPTATELEGFTEDLARRRALGDDEARLAATAG
                     VGAPMSGLRSAASALAARWGFAPWLEADPDRPRQDALTLAAVFPAIAAAVSGHAETIP
                     SLGEDGHVAAYVKAVTGGGADERQVTAMNRYFSLTADHGMNASTFTARVIASTGADVG
                     GAVAGAIGALSGPLHGGAPSLVLDMLDEIGSVDRARDWLAERVRAGHRLMGFGHRVYR
                     TDDPRALALRRTAQELGGGRTELALAVEDAAHEVLRELKPGRPIYTNVEFYSAVVFEL
                     AGIPRPMATVTFACSRVVGWIAHVLEQIADNRLIRPTATYLGEMPDYRD"
BASE COUNT          425 a         1261 c         1254 g          415 t
ORIGIN      
        1 tccatgccga actcgccctc gcagaacgtg atcagctgct cgatctcgag cgagtcgagc
       61 aggccggcgg cgaggggatc gaggtcggat tcctccgcct gcgcatccgg ccgggcggcg
      121 aggtaggcgt ggatccggtc actggccttc atccgatcag ctcatcgcgg ggacagctcg
      181 tgcaggcggg agcgcgccca ggccacgacg cgctcccgcc cctcgcgctg tcccccgagc
      241 gtgcggttcc cggcgccgct gaaggtctgc acctcgagcg ggaccccctc ctcgcgcagg
      301 gtccgctgca tggtcatgaa ggcgggggtg cggccatcgc cgtcggcgac gatcgccagg
      361 gaggggacgg tccggaacgc gccccggaag acgtccttca gggagcggtc gaaccgttcg
      421 cgcggcgacc ggccggccag gcgggtcact gcgcggcgag cgcgggccgt gaacttcttg
      481 ctcggcggga tgcgcacgta ggggttgacg agcgccaggc cgaccacgtt cgggacctcg
      541 gtggccaccg ccgccgccag gcgcgacccg aagcaggccc cgatgaacag caggtcgacg
      601 tcgccgacgc gctcgcggaa ccaggcgatg acctcgcgca tcggcaggcc gggggggatc
      661 cccggcggaa cggcccagct gtcgccccag cccgggtaat cgaggcgcag caccgtgacg
      721 cccatccccg ccagctcgcg ggcggtcagc gtccagacct ggttgacgcc ggagcgccga
      781 ccctcgcgcc cctcgaacag gatcgcggcg gcgcggcgtg gattgccgtc gggctcggtg
      841 atgatcgcgc cgatcagccc gtggcctgtg ttgacgagga ccgggtgcgt gctcatgcgg
      901 ccgcaaccgc cgcggccacc caatgaccga accccgtcac cagggcctcg acgttcgtgg
      961 cgtgcaccgg ccggcgccat tcgccgggca cgacgtgggt ctcgacgcgc tccgggagcg
     1021 gtggacgctc cggcagccgg tgccggaacg cggccacccg gccgccgaac gactccaggg
     1081 cgccagcaac gtccagcgcg gcggcgctcg accggatgcg ccgggtgagc gggtacccga
     1141 acacggtcgg ctcggggtcc acccgcccga gggcggcgcg gcgcgcggcc cgctcgagca
     1201 cgccgaggtg cgccgccggg tcgagggccg gctcgacgag cgggaggatg cgcccgttcc
     1261 tcgccgcggc gagcacggcc accgcggcgc cgaggtcgac cccgccgaac tcccaggcga
     1321 cctccggcac gctgctcacg gcgagcgcca gcgcgtcgcg ggcggcctcc gccagccgct
     1381 cgaacgtggc ctcctcgaac cggccgccgc tgtcggcgtg ccccggcggg tggtacgcca
     1441 ggccggcgcc gccgcgggcg gcgatcgcgt gcgccagccg gtgggtgagc tcgagctgct
     1501 ccatgacgtc gtgcccccac gacgggcaca cgacgacccc caccggcagc cgtggcggtt
     1561 cggcggcgta gagcgatgcg tagatctcgt cccgaccgga ggggaagaag gtgacctcgc
     1621 gcctgaggcc gtcccccgtc cacgtcgagg cggacgccca ttcctgcatg cggtcccgaa
     1681 tcttagtgga ccggcgggcg gtcacggtcc gccactcggc ggccgcgccg gaggtccggt
     1741 cgcgggtcgc gcgacggagc gggcggggct acgatcgcga cggatgcccg acgtcgtcgt
     1801 gcgcgacctg ctgacccccg atccggtcac cgtccgcccc gcggacccga tcggggtcgc
     1861 cgcgaacgcg atgcgcgccc ggggggtcgg ctcggcgctc gtcaccgatg acggccggct
     1921 ggtcgggatc atcaccgagc gcgacatggt cctctccacc gctggcggcg cgcggcccga
     1981 cacctccacg gtggcggcct ggatgacgcc cgacccggtc accgtctcgg ccggaacctc
     2041 cgccgacgtg gctctcgacc tgctctccca gaaggggttc cgtcacctgc cggtggtgga
     2101 cgacggcaag ctggcgggga tcgtctcgct gcgcgacctg gtggcggggc tgcgtgtgac
     2161 ccgcgtcgac ccgacccggc cctcgaccgc caagggcctg gagaacgtgg tggtcggcga
     2221 gacgcgcaag agcttcatcg acggggagcg gggccggctg gtctacgtcg gccgcgacgc
     2281 cgtcgagctg gcgctgaacc acccgttcga ggcggtctgg ttcctgctca tggaggagcg
     2341 actgccgacg gccaccgagc tggaggggtt caccgaggac ctggcgcggc ggcgcgcgct
     2401 cggcgacgac gaggcgcgcc tggcggcgac cgccggggtc ggcgccccga tgtccgggct
     2461 gcgctccgcg gcctcggcgc tcgccgcgcg ctgggggttc gcgccctggc tggaggccga
     2521 ccccgaccgc ccgcgccagg acgccctgac cctggcggcg gtattcccgg cgatcgccgc
     2581 ggcggtgtcc ggccacgcgg agacgatccc ctcgctcggc gaggacggcc acgttgccgc
     2641 ctacgtcaag gccgtcaccg gaggaggcgc cgacgagcgg caggtgacgg cgatgaaccg
     2701 ctacttctcg ctgaccgccg accacggcat gaacgcgtcc acgttcaccg cccgggtgat
     2761 cgcctcgacc ggcgcggacg tcggcggcgc cgtggccggc gccatcggcg cgctgtccgg
     2821 gccgctgcac ggcggggcgc cctcgctcgt cctcgacatg ctcgacgaga tcggttcggt
     2881 cgaccgggcg cgcgactggc tggcggagcg cgtccgcgcc gggcaccggc tgatgggctt
     2941 cggccaccgc gtctaccgca ccgacgatcc gcgcgcgctg gcgctgcgcc ggacggccca
     3001 ggagcttggc ggtgggcgga ccgagctggc gctcgcggtc gaggacgccg cccacgaggt
     3061 cctgcgcgag ctgaagcccg gccggccgat ctacacgaac gtcgagttct actcggccgt
     3121 cgtcttcgag ctggccggca tcccgcgccc gatggccacc gtgacgttcg cctgcagccg
     3181 ggtggtgggg tggatcgcgc acgtcctgga gcagatcgcc gacaaccggc tgatccgccc
     3241 caccgcgacc tacctcggcg agatgcccga ctatcgcgac tgaggcgccg tgcccaccgg
     3301 catccccgcc tcggcccagc gcgccagctc cccggtcgcg taggcgtcgc gcacg
//
LOCUS       DASPWO010000031         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_4380, whole genome shotgun sequence.
ACCESSION   DASPWO010000031 DASPWO010000000
VERSION     DASPWO010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4380"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VEO00_00715"
     CDS             complement(<1..581)
                     /locus_tag="VEO00_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56556.1"
                     /translation="MGAPTSLIDAPERPRAGLRRRLAGILPAPSSPRPWYERVEAWSG
                     VAVLAACCLFLFFQQQPHLLLRDTTTAGGDTGAHVWWPAYLRDHLLPHWRLAGWTKDF
                     YAGFPAGQFYFPLPALLIVVLDVVLPYNVAFKLVTALGPIILPIGAYVFGRGLRFPRP
                     APAAMAVAATAFLFFKGAPGSSARATSQAFNQR"
     gene            complement(646..819)
                     /locus_tag="VEO00_00720"
     CDS             complement(646..819)
                     /locus_tag="VEO00_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56557.1"
                     /translation="MRCTTCSKGALVEIRMRIGGSDVTFRRCGRCESQAWETVEGPVA
                     LTEVLDLARATAT"
     gene            complement(902..1621)
                     /locus_tag="VEO00_00725"
     CDS             complement(902..1621)
                     /locus_tag="VEO00_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HYU56558.1"
                     /translation="MDASAAATRVRWGIPDAALAWFLGLLAATVAGGVAAGIIDVPSD
                     ELADHLGYLLVVIAAQGAATIAFLALVARRKGRGSLARDFGLVVRLRDVGWVGVGSLL
                     ELAAVIALQPIVEVYGREEDQGVVDQLKDARGVGLALFVVAVLVLAPVAEELLFRGVL
                     LRALLRRTSPGWAMFVSAAVFALVHPLGDPEIGSVIAVPAVLTLGLVSGYQAIRTGNL
                     SRSICLHAGFNLLTVLVVLTD"
     gene            1933..>2582
                     /locus_tag="VEO00_00730"
     CDS             1933..>2582
                     /locus_tag="VEO00_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HYU56559.1"
                     /translation="MPWILIAVLVGGFLLLNVAGGGSDKKELTYSQVRAAVRDDKVGT
                     IEYDPSNGKITGKFTKEQDGKEEFSSSGPNDNFTEEDLALFREHDVDVKYKREGDSIL
                     GQVLFWVLPLGLLVLLFVWISRRQAGQMGAVMNIGRSRAKVYSTEKPKTTFADVAGYG
                     PVKAEIAEVVDFLKFPQKFKQIGARIPKGVLLVGPPGTGKTLIARAVAGEAGVPFVS"
BASE COUNT          448 a          862 c          964 g          308 t
ORIGIN      
        1 atgcgctggt tgaacgcctg ggaggtcgcc ctcgcgctgc tgccgggcgc acccttgaag
       61 aagaggaagg cggtcgccgc aaccgccatc gccgccggcg ccggccgggg gaaccgcaga
      121 ccgcgtccga agacgtaggc cccgatcggg aggatgatcg gcccgagcgc ggtgacgagc
      181 ttgaacgcga cgttgtatgg gaggacgacg tcgaggacga cgatcaggag cgcgggcaac
      241 gggaaataga actgaccggc ggggaagccg gcgtagaagt ccttcgtcca acccgccagc
      301 cgccagtgcg ggaggaggtg gtcgcgcagg tacgcgggcc accacacgtg ggcgccggtg
      361 tcgccgccgg cggtggtcgt gtcgcggagc agcagatggg gttgctgctg gaagaacagg
      421 aagaggcagc aggcggcgag gacggcgacg ccggaccagg cctcgacccg ctcgtaccag
      481 ggtcggggtg agctcggagc ggggaggatg cccgccaggc gtcggcgcag gccggctcgg
      541 gggcgctcgg gcgcgtcgat cagcgaggtc ggcgcgccca tgggtgccag cttggcaccc
      601 gggtcgggcg gggcccgatc aaccgcccgt gcggagcgac gcgagttagg tggcggtggc
      661 gcgggccagg tcgaggacct cggtcagcgc gaccggacct tcgaccgtct cccacgcctg
      721 gctctcgcag cggccacagc gtcggaacgt gacgtccgag ccgccgatcc tcatccggat
      781 ctcgacgagg gctcccttgg aacaggtggt gcaacgcatg tccagcctcc ctttgcaacc
      841 tcagccgtcg agagcaacgg cagatcggcc ggaaaacttg agccggaatc gcgcacatgg
      901 actagtccgt gagcacgacc agcacggtca gcaggttgaa gccggcgtgg aggcagatcg
      961 agcgggacag gttgccggtc cggatcgcct ggtaccccga gacgagcccg agggtgagga
     1021 cggccgggac cgcgatcacc gatccgatct cggggtcgcc cagcgggtgg acaagggcga
     1081 agacggcggc cgagacgaac atcgcccagc ccggactcgt ccgacggagc agcgcccgca
     1141 ggagcacccc ccggaacagg agctcctcgg cgacgggggc gagcaccagc accgcgacga
     1201 cgaacagggc gagcccgacg ccgcgggcgt ccttgagctg gtcgacgacc ccctggtcct
     1261 cctcccggcc gtacacctcg acgatcggct ggagcgcgat cacggccgcg agctcgagca
     1321 ggctgccgac gccgacccag ccgacatccc tgagccgcac gacgagcccg aagtcccggg
     1381 cgagcgagcc ccggcccttc cgcctcgcga ccagcgcgag gaaggcgatc gtcgccgcgc
     1441 cctgcgccgc gatgacgacg aggagatacc cgaggtggtc ggcgagctcg tcggacggca
     1501 cgtcgatgat cccggccgcg acgccaccgg cgacggtggc ggccaacagg ccgaggaacc
     1561 acgcgagcgc ggcgtcggga atgccccaac ggacgcgggt ggcggcggcc gacgcgtcca
     1621 tcgagctcaa ggtaccggag cgcggtgcgc cgatcgggga gggcgccaaa ccgaaacagc
     1681 cggaaataga aatgcccccc gaagtgactg accttgcggc ccgccccttc ggaggacgca
     1741 gggaacgtac cacgggcctg cggggcggtc aagggtgcga ttcgggcccg ggacggcggc
     1801 accggccgct ggccgtacga tggcacgatg agtcggcccc cctccgagat gccgccgggg
     1861 cgtccccaac ggcccggtcc cccgcggccg ggcccgggcg ggccgccgac accgtcgccc
     1921 tggacccgct gggtgccgtg gatcctcatc gccgtgctcg tgggcgggtt cctcctcctg
     1981 aacgtggccg ggggtggctc cgacaagaag gagctcacgt actcccaggt gcgggcggcg
     2041 gtacgggacg acaaggtcgg gaccatcgag tacgacccgt caaacgggaa gatcaccggc
     2101 aagttcacga aggaacagga cggcaaggag gagttctcga gctccggtcc caacgacaac
     2161 ttcaccgagg aggacctcgc gctcttccgg gagcacgacg tcgacgtgaa gtacaagcgc
     2221 gagggcgaca gcatcctcgg ccaggtcctg ttctgggtgc tgccgctcgg cctgctggtg
     2281 ttgctgttcg tctggatcag ccggcgccag gcgggccaga tgggcgcggt catgaacatc
     2341 gggcgcagcc gggcgaaggt gtactcgacc gagaagccga agacgacgtt cgccgacgtc
     2401 gccgggtacg ggccggtcaa ggcagagatc gccgaggtcg tcgacttcct gaagttcccg
     2461 cagaagttca agcagatcgg cgcgcgcatc cccaaggggg tcctcctcgt cggaccgccg
     2521 ggtaccggca agacgctcat cgctcgcgcc gtcgcgggcg aggctggcgt gccgttcgtg
     2581 tc
//
LOCUS       DASPWO010000032         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_174771, whole genome shotgun sequence.
ACCESSION   DASPWO010000032 DASPWO010000000
VERSION     DASPWO010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174771"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VEO00_00735"
     CDS             complement(<1..402)
                     /locus_tag="VEO00_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018769708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56560.1"
                     /translation="MSAIDELRVSFRTAKDPVAFLRKRSGLPGPRGNLELLHAFVEEA
                     PPALIRAFAALGPDAAPENTPDGFVACCGVAGLGRLAAEGDRRALPELRRHASDPRWR
                     VREGVAMALQRLGETDPRRLVREMRRWAGGTA"
     gene            complement(424..597)
                     /locus_tag="VEO00_00740"
     CDS             complement(424..597)
                     /locus_tag="VEO00_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56561.1"
                     /translation="MATVPRSRIPTFRDGDAHVAIASNAGEERDRVLNLRADPRATLQ
                     VGRPVILLEPEGS"
     gene            complement(798..1208)
                     /locus_tag="VEO00_00745"
     CDS             complement(798..1208)
                     /locus_tag="VEO00_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU56562.1"
                     /translation="MSSEFPAEGMELTHILVVRDPARSRDFYRDVLGAELYRQYGTSV
                     VLRFLGGWLLLVTGGGPTEDKPTVTFAAPDEIDRVDHAFTIRVPDCRAAHATLAARGA
                     PFLTPPVESAWEVRAFFRDPDGHLWEISEARSDG"
     gene            complement(1342..2178)
                     /locus_tag="VEO00_00750"
     CDS             complement(1342..2178)
                     /locus_tag="VEO00_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56563.1"
                     /translation="MAGLDDRIDELYAMPPEEFIAARNALATELKAAGDERAAEVKAL
                     RRPSVAAWAVNSLAREEPTRLNELVEAGRAMRTAQGKLLKGRGAEDLEEAAGRRRRLV
                     VELARRAGALLRDAGRPSSATLLDAVEATLLATATDEVAAKRVLHGRLEKELPTPAGF
                     GGLIAPGPADEPPAAALAKKTRKATVAAAPSARERRERERARGRADEAGVRAEEAEAE
                     AGRLREAATAAERAAAEAAREADRARRAADRARAAAERAANRAADLRERADRARAAVE
                     KE"
     gene            2283..2771
                     /locus_tag="VEO00_00755"
     CDS             2283..2771
                     /locus_tag="VEO00_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56564.1"
                     /translation="MPVQISQGGNRFALGQTESAYAIWPSTGGKPVQKWPKNDEGWAA
                     AYREFQRLEGESGDHYAPDVSLPASSPIVVVQGGSGLGTAALVLGIVGAAFGLIPLFA
                     IIALICGAIAVVFGAIGLKRPGRGRAIAGLVLGLVALALGIWGAFIVNEAVNELEQQL
                     GS"
     gene            complement(2813..>3235)
                     /locus_tag="VEO00_00760"
     CDS             complement(2813..>3235)
                     /locus_tag="VEO00_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012751003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HYU56565.1"
                     /translation="TRLRAKGIDAAALSGDMTQSARERSLKRFEQGRVHALVATDVAA
                     RGLDLTRVGHVVNFDPPEDDKGYVHRVGRTARAGRSGVGVTFVLPDQVSDVSRMAARL
                     QLADEFRAEGMQVAPPRVVYQSRKGRRSLLAGRPRRTS"
BASE COUNT          413 a         1204 c         1169 g          449 t
ORIGIN      
        1 cgcggtgccg ccggcccagc ggcgcatctc ccgcaccagg cgccgcgggt cggtctcgcc
       61 gagccgctgc agcgccatcg ccacgccctc gcggacgcgc cagcgcggat cggaggcatg
      121 acggcgcaac tccggaaggg cgcggcggtc gccctcggcc gccagccgtc ccagcccggc
      181 gaccccgcag cacgccacga acccgtccgg cgtgttctcc ggggcggcgt cgggccccag
      241 cgcggcgaac gcccggatca gcgcgggcgg agcctcctcg acgaacgcgt ggagcagctc
      301 caggttgccc cgcggccccg gcagtccgga acgcttccgc aggaacgcca ctggatcctt
      361 cgccgtccgg aacgacaccc gcagctcgtc gatcgcgctc acggaccagc gccgccggcg
      421 gcgtcaggag ccttccggct cgagcaggat caccgggcgg ccgacctgca gcgtggctcg
      481 cggatcggcc cgcaggttca acacccgatc acgttcctcg ccggcgttcg acgcgatcgc
      541 cacgtgcgcg tcgccgtcgc ggaaggtcgg gatccggctc ctgggcaccg tggccatcgc
      601 tgtgccgaag cctacgtgcc ggcgaaccgc tcgggttgcc gcccggggtc gggtctgacg
      661 gcggtcggcg agcgtcttcg gcaaaaaacg gaaccgccaa agcccgaagg ctccagcggt
      721 tccaaaaccg acgatagggg acgccgcccg cacacgcaac ccggggcccg ggttttgtca
      781 ctctttctcg cggcggctta gccgtccgag cgggcctcgc tgatctccca gagatgtccg
      841 tcgggatcgc ggaagaacgc gcgcacctcc cacgccgact cgaccggcgg cgtcaggaac
      901 ggggcgccgc gcgcggcgag cgtggcgtgg gcggcccggc aatcggggac gcggatcgtg
      961 aacgcgtggt ccactcggtc gatctcgtcg ggcgcggcga acgtgaccgt cggcttgtcc
     1021 tcggtcggac cgccgccggt gaccagcagc agccagccgc ccaggaagcg caacacgacc
     1081 gaggttccgt actggcggta gagctcggcg cccagcacgt ctcgatagaa gtcacgcgat
     1141 cgagcggggt cgcgaacgac gaggatgtgc gtcagctcca tcccctcggc cgggaactcg
     1201 ctgctcaccc acccgccttc cgcgcccgtg gccggcgctc gggcccgagg tgaaaccgcc
     1261 ggagcccgaa ggctccagcg gtctcgaggc cggacggtac gggatcgtgg cccggccgcg
     1321 cacgtcgcgg agcggaggtc gtcactcttt ctccactgcg gcgcgcgccc ggtcggcgcg
     1381 ttcgcgaagg tcggccgcgc ggttggcggc ccgctcggcc gcggcccggg ctcggtcggc
     1441 ggcccggcgg gcccggtcgg cctcccgggc cgcctctgcc gcggcgcgct cggcggcggt
     1501 ggcggcctct cgcagccggc cggcctcggc ttcggcctcc tccgcccgta cgcccgcctc
     1561 gtccgcgcgc ccccgggccc gttctcgttc ccggcgttcg cgtgccgacg gcgccgcggc
     1621 caccgtcgcc ttccgcgtct tcttcgcgag ggcggcggcc ggcggctcgt ccgcagggcc
     1681 gggcgcgatc agccccccga agccggcggg ggtgggcagc tccttctcca gccggccgtg
     1741 caggacgcgc ttcgccgcga cctcgtcggt ggcggtggcc aggagggtgg cctcgaccgc
     1801 gtcgaggagc gtggccgacg agggacggcc ggcgtcgcgc agcagcgctc cggcccgccg
     1861 ggccagctcg acgaccagac ggcgccgtcg cccggccgcc tcctcgaggt cctcggcgcc
     1921 ccggcctttc agcagcttcc cctgagccgt ccgcatcgcc cggccggcct cgacgagctc
     1981 gttcagccgc gtcggctcct cccgggccag gctattcacg gcccacgctg cgacgctcgg
     2041 ccggcgcagg gccttcacct cggccgcccg ctcgtctccg gccgccttca gctcggtggc
     2101 cagcgcgttg cgcgccgcga tgaactcctc cggcggcatc gcgtacagct cgtcgatccg
     2161 gtcgtccagc ccggccacga ccagcagtgt tccccctgcg gcgccgtttc accagccggg
     2221 cgagacgtgc cgattgacgg gggccgatgc gaggttgcac gatcacattc gggggatgcc
     2281 cgatgccggt tcagatcagc cagggaggca atcgattcgc gctcggacag actgagagcg
     2341 cgtacgcgat atggccgtcg accggtggta agccggttca gaagtggccg aagaacgatg
     2401 agggatgggc cgccgcgtat cgggaattcc agaggctcga aggcgagagc ggtgaccact
     2461 acgctccaga cgtgagcctc ccagcgagct cgccgatcgt ggtcgtgcaa ggtggcagcg
     2521 gtcttgggac agcggcgctc gtcctcggga tcgtcggggc tgccttcggc ttgatcccgc
     2581 tcttcgcgat catcgcgttg atctgcggcg ccatcgcggt ggtcttcggt gcgattggtc
     2641 tgaagaggcc gggcagaggc cgagcgatcg caggtctcgt cctaggactc gtggccctgg
     2701 cactcgggat ctggggtgcc tttatcgtca atgaggcggt caacgagctc gagcaacagc
     2761 tcgggtcctg aaggaactct ccggctagct agcacccgga cgggcgactg cgtcagctgg
     2821 tgcggcgggg gcggccggcc aggagggagc ggcgcccctt gcgggactgg tagacgactc
     2881 gcggcggggc gacctgcatc ccctccgccc tgaactcgtc ggccagctgc aggcgggcgg
     2941 ccatgcggct gacgtcggac acctgatcgg ggaggacgaa cgtgacgccg acgccggagc
     3001 gcccggcgcg ggccgtgcgg ccgacgcggt gcacgtagcc cttgtcgtcc tcgggcgggt
     3061 cgaagttcac cacgtgcccc acgcgggtca ggtcgagccc gcgcgccgcc acgtcggtgg
     3121 cgacgagcgc gtggaccctg ccctgctcga atcgcttcag agaccgctct cgcgccgact
     3181 gcgtcatgtc gcccgacagc gcggccgcgt cgatgccctt cgcccgcagc cgggt
//
LOCUS       DASPWO010000033         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_683833, whole genome shotgun sequence.
ACCESSION   DASPWO010000033 DASPWO010000000
VERSION     DASPWO010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683833"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VEO00_00765"
     CDS             complement(<1..865)
                     /locus_tag="VEO00_00765"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYU56566.1"
                     /translation="MRVNTDCGRAGQQEEWVAVNPTDPPNVIVSQTGAVTGVHYSLQG
                     GASGSFGDSSVFMDLVPCDPANPLCGPGGYWSYDLFTDPTHAFDPEGNLYFAAIGFDF
                     LRDPLNGTYVWKGNACGKGQALGNPSDDRCFPIDGFAAVIHDNFAHPMDQDATPWLSS
                     GWLPGDPGKSQVAAVWTTSQFGCGPGGSDLCSQPGFISRSTDGGVTWTRPMEVTGQAP
                     FCVGGDAFTGRPTDAHACNLDEWIHHVIAPDGSIYVVWLNYNTPTLVNQLLFRRSTDS
                     GATWSRVYRVAD"
     gene            complement(1237..1310)
                     /locus_tag="VEO00_00770"
     tRNA            complement(1237..1310)
                     /locus_tag="VEO00_00770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1274..1276),aa:Arg,seq:tct)
     gene            1453..2466
                     /gene="gnd"
                     /locus_tag="VEO00_00775"
     CDS             1453..2466
                     /gene="gnd"
                     /locus_tag="VEO00_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HYU56567.1"
                     /translation="MQLGMVGLGRMGSNLVRRLMRGGHECVVYDVSHDAVRALQSEGA
                     LGATSLDDFAAKLTPPRAAWVMVPAAFAGQTVLALAERMEEGDIVIDGGNSYYRDDIE
                     RGRDLAARGMHFVDVGTSGGVFGLDRGFCLMIGGEADVVRRIDPIFATLAPGVDAAPR
                     TEGRRREGTAERGYLHCGPTGAGHFVKMVHNGIEYGIMAAYAEGLNILRKANIGSKPA
                     AADAETAPLEHPEFYRYDIDTADVAEVWRRGSVVASWLLDLTASALAKDPDLAGFSGR
                     VSDSGEGRWTVGAAVDEAVPAYVLTAALYERFSSRGEADFANRLLSAMREEFGGHAER
                     VDG"
     gene            complement(2473..>2911)
                     /locus_tag="VEO00_00780"
     CDS             complement(2473..>2911)
                     /locus_tag="VEO00_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYU56568.1"
                     /translation="VIAKRADSPYESRRSTAWLKFKCVNEQEFVIGGYTDPKGSRTGL
                     GALLLGYYEGGTLVYAGKVGTGFDTRLLQELAAKLALLERPDPPFERGKLPTKGVHWV
                     EPKLVGQIGFSEWTVDGQLRHPRFLGLREDKPPREVVRERPTA"
BASE COUNT          461 a         1002 c          997 g          451 t
ORIGIN      
        1 catctgccac ccggtacacc ctcgaccagg tcgctccgct atcggtggat cgacgaaaca
       61 gcagctggtt cacgagcgtc ggcgtgttgt agttcagcca caccacatag atcgagccgt
      121 ccggggcgat cacgtggtgg atccattcgt ccaggttgca cgcgtgcgca tccgtggggc
      181 ggccggtgaa cgcgtccccg ccgacgcaga acggagcctg gcctgtcacc tccatcggcc
      241 gggtccaggt caccccgccg tccgtggatc gcgagatgaa tccgggctgc gaacagaggt
      301 cggacccgcc cgggccgcag ccgaactgcg acgtggtcca gaccgccgcg acctggctct
      361 tccccgggtc acccggcagc cagccggagc tcagccacgg cgtggcgtcc tggtccatcg
      421 ggtgggcgaa gttgtcgtgg atgaccgctg cgaacccgtc gatggggaaa caccggtcgt
      481 cggaagggtt tccaagcgcc tggcccttcc cgcaggcgtt gcccttccac acgtaggtgc
      541 cgttgagcgg gtcgcgaagg aagtcgaatc cgatggccgc gaagtacaga ttcccctccg
      601 ggtcgaaggc gtgagtcggg tcggtgaaca ggtcgtacga ccaatatcca ccgggaccgc
      661 agagtggatt ggcaggatca cacggcacca gatccatgaa cacgctggag tccccgaagg
      721 agccggaagc gccgccctgc agcgagtaat ggacgccggt gaccgcaccc gtctggctga
      781 cgatcacgtt cggcgggtcc gtgggattga cggccaccca ctcctcctgc tgcccggcgc
      841 gtccgcagtc cgtgtttact cgcacattgt ccggaaaccc cgagcggcga aaggtcttcg
      901 tacatgccgc cagggcgccg cgtcgatggt cagcgaggga ggcggctccc ctcgcctggc
      961 gccgaagcga gagcagccct ccgctgggca ggcgcaccac atcagctgat ccggttcctc
     1021 ctcggatggc tgccaggcgg tgatccgatc ctcccgtggc ccgaccgatg ccacccgtcg
     1081 tcagaacggc gagcaacccg gcgatgacga cgatcatctg tgtggtcgga cgtctcattc
     1141 gagcccccgg cgggagtgtg gaccgtcggc gaggcgaagg caagaagctg tcgtcgtagc
     1201 atccacggcg cctactggaa ccaacaacca ggtcagtgcg ccgggaggga ctcgaacccc
     1261 cgacccgctg cttagaaggc agccgctctg tccacctgag ctaccggcgc gtccgctacc
     1321 gattctagct gcggggatac ggccttctcg gtggtcccgg cccgacggtt gaggctccac
     1381 ggcggacgga ccgcgtctgc ccctgatgct agcctgccct gacgctcacg cgccttgagg
     1441 aggcgatcgc ggatgcaact cggcatggtc gggcttggtc ggatggggtc gaacctcgtg
     1501 cgccggctca tgcgcggcgg gcacgagtgt gtcgtctatg acgtcagcca cgacgccgtc
     1561 cgcgccctgc aatccgaagg ggcgctgggc gcgacctccc tcgacgactt cgcggcgaag
     1621 ctcacccctc ctcgcgccgc gtgggtgatg gtccccgccg cgttcgccgg ccagaccgtc
     1681 ctggccctcg cggagcggat ggaggagggc gacatcgtga tcgacggtgg caactcctac
     1741 taccgtgacg acatcgaacg ggggagggac ctcgccgccc gcgggatgca cttcgtcgac
     1801 gtggggacca gcgggggcgt gttcggtctg gaccgcgggt tctgcctgat gatcggggga
     1861 gaggccgacg tggtccgccg catcgacccg atcttcgcca cgctggcgcc cggcgtggac
     1921 gcggcccccc ggaccgaggg ccgccggcgc gaagggaccg ccgagcgggg ctacctccat
     1981 tgcgggccca ccggcgccgg gcacttcgtg aagatggtcc acaacgggat cgagtacggg
     2041 atcatggccg cctacgcgga gggtctgaac atccttcgga aggctaacat cggctcgaaa
     2101 ccggccgccg ccgacgcgga aaccgccccg ctcgagcacc ccgagttcta ccggtacgac
     2161 atcgacaccg ccgacgtggc ggaggtgtgg cggcggggga gcgtggtcgc ctcgtggctg
     2221 ctggacctca cggcatcggc cctggcgaaa gaccccgatc tggcggggtt ctctggacgg
     2281 gtctcggact ccggcgaggg ccggtggacc gtcggcgcgg ccgtcgacga ggcggtgccc
     2341 gcgtacgtcc tgaccgccgc gctctacgag cggttcagct cacgcggtga ggccgacttc
     2401 gccaaccgcc tgctctccgc gatgcgcgag gaattcggcg gccacgccga gagggtcgac
     2461 ggatagcgac cgttacgcgg tcgggcgctc ccggaccact tcgcgcggag gcttgtcctc
     2521 ccgcaatccg aggaaccggg gatggcgaag ctggccgtcg acggtccact cgctgaagcc
     2581 gatctgcccc acgagcttcg gctcgaccca gtgcacgccc ttcgtcggaa gcttgccccg
     2641 ttcgaagggc gggtcggggc gctccagcaa ggccagcttc gcggcgagtt cctgaagcag
     2701 ccgggtatcg aatcccgtcc ccaccttgcc cgcatagacg agagtcccgc cctcgtagta
     2761 cccgagcagc agcgcgccca ggccggtccg gctgccctta gggtcggtgt agccgccgat
     2821 cacgaactcc tgctcgttca cgcacttgaa cttgagccac gccgtcgacc gccgggattc
     2881 gtagggcgag tccgcgcgct tggcgatgac g
//
LOCUS       DASPWO010000034         3994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_258933, whole genome shotgun sequence.
ACCESSION   DASPWO010000034 DASPWO010000000
VERSION     DASPWO010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258933"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VEO00_00785"
     CDS             <1..932
                     /locus_tag="VEO00_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HYU56569.1"
                     /translation="TNSIRLLVGLPGGDSGVRDLARDMVITRLGQAVDRTGRLDPDAL
                     ARTLAVLERYARRARALGARRMRVGMTSAVRDAANRDDLVREVRRIAGSDPEILSGEQ
                     EAALSYLGAVRTVDAEGPFLVIDIGGGSTELVTGGERAQAAISLDVGSVRLTERHVRH
                     DPPEPAELAAMTADLDRRLEAVLGAIPLEGARTFVAVAGTATTVAALALDLPGYDPDR
                     IHGSTLTLEDARTVRDRLASMTTLQRAALPVMAPGREDVIVAGAAILVRAMERLGFAQ
                     AVVSEHDILDGLAIEVLEGTPGSGHPFEGGESR"
     gene            944..1735
                     /locus_tag="VEO00_00790"
     CDS             944..1735
                     /locus_tag="VEO00_00790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYU56570.1"
                     /translation="MARNRSRRRQLQKAAERRAAERRRQRRQRILTIAVGVAVAAAGG
                     TVAFFALTGGEGENPRSGPTTASPTPEVVACGGQVPAAAGEEKPVYDRPPEMQIDRSK
                     TYTATMVTTCGTIRLRLDAKGSPQTVNSLVFLARKRFFDGLKFHRIVKNFVIQGGDPK
                     GDGTGGPGYSTVDTPPKGLKYEVGDLAMAKTQAEAPGTAGSQFFIVTGDPAPLNASPV
                     YALVGTVVEGLDVAQTIEQLPAVGGATDGVPADAVYIESVTIEER"
     gene            1831..2979
                     /locus_tag="VEO00_00795"
     CDS             1831..2979
                     /locus_tag="VEO00_00795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU56571.1"
                     /translation="MGRALYVLPRAQRRGAAMTSTRLAAQLRGEWEPLVVTLFTGGDP
                     SVLGAAVPIRQLEVRAGLGRRVLGVSPAAVRALRAAALEFRPDLLVAWGGEPLLHAAP
                     VARRAGARLLYVKISPTQPKLVRGLRRIVFRRALARADWLVAVTVELAEELRRDLGVR
                     RPVAVAHNFRLPTDRPSPGDRASFRAELGVGDDVVLAAFAGSLSRDKGPDQALEALAL
                     ATGNVHLAVAGAGPLEGALRERAARPDLAGRVHLLGSRGDVPRILAGADLVLLSSREE
                     GRPGVLLEAALAGRPAVAFAEGGVAALVDDGVEGVLVAPGDVAALGRALSALAADPAR
                     RQAMGSAAARRGDGYLAPAALPAWREAFRAATAGRRSSAPRSSEPGPG"
     gene            complement(2924..>3994)
                     /locus_tag="VEO00_00800"
     CDS             complement(2924..>3994)
                     /locus_tag="VEO00_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HYU56572.1"
                     /translation="GLLAGADVTVICVPTPLADHEPDLSFVRAAATKVAKHLRPGTLV
                     VLESTTYPGTTEEELRPILETSGLVAGVDFALAYAPERIDPGRETAHVVTTPRVVGGI
                     GPRSTEVAAAFYRTLVNEVITVRSPREAEMAKIIENTFRHVNIGLMNELAMLSRDLGV
                     DIWEAIGAAATKPFGYMAFWPGPGVGGHCIAIDPSYLSWRVGQRLGHRPRFIEHAQEV
                     NAKMPAYVAGRIGEALNAHGKALRKAKVLVLGLTYKADVNDIRESPAVVVVERLLKSG
                     ATISYHDPFVPDIRIGTRKLSSVKLTPAALERADCVVILTPHSAVDYADVVARSQLVF
                     DARGVTRGLAPKSAAPKNVVLL"
BASE COUNT          492 a         1444 c         1538 g          520 t
ORIGIN      
        1 ggacgaactc gatccgcctg ctggtcgggc tcccgggcgg cgatagcggg gtgcgcgacc
       61 tggcccgcga catggtcatc acccggctgg ggcaggccgt cgaccgcacc ggccgcctcg
      121 accccgacgc cctggcccgc acgctcgccg tcttggagcg ctacgcgcgg cgggcgcgtg
      181 ccctcggcgc ccggcggatg cgggtcggga tgacctcggc cgtgcgcgac gccgcgaacc
      241 gcgacgacct ggtgcgcgag gtccgccgca tcgccggctc ggacccggag atcctgagcg
      301 gcgagcagga ggcggccctg tcctacctgg gcgcggttcg gacggtcgac gcggaggggc
      361 ctttcctggt gatcgacatc ggcggcggga gcaccgagct cgtcaccggc ggggagcggg
      421 cccaggcggc gatcagcctc gacgtcggaa gcgtccggct caccgagcgc catgtccggc
      481 acgacccgcc cgagccggcg gagctcgcgg cgatgaccgc cgatctcgac cgccggctgg
      541 aggcggtcct cggagccatc ccactggagg gggcgcgcac gttcgtcgcc gtggccggga
      601 cggcgacgac ggtcgccgcg ctggccctgg acctccccgg ctatgacccg gaccggatcc
      661 acggttcgac gctcaccctc gaggacgccc gcaccgtgcg cgaccgcctg gcttcgatga
      721 ccaccctcca gcgcgcggcg ctcccggtga tggcccccgg gcgagaggac gtgatcgtcg
      781 ccggcgccgc catcctcgtc cgggcaatgg agcgcctcgg tttcgcccag gcggtggtct
      841 ccgagcacga catcctggac ggcctcgcca tcgaggtcct ggagggaacg ccgggatcgg
      901 gccacccctt cgagggcggg gaaagtcgct aaccttcccg cccgtggcac gcaaccggtc
      961 ccgccggcgc cagctgcaga aggccgcgga gcgccgcgcc gctgagcgcc gccggcagcg
     1021 ccgccagcgc atcctgacga tcgcggtcgg ggtggccgtg gccgccgcgg gcggcacggt
     1081 ggcgttcttc gcgctcaccg gcggcgaggg cgagaacccg cggtccggcc ccacgacggc
     1141 gtcgcccaca cccgaggtcg tggcgtgcgg agggcaggtc ccggccgccg ccggagagga
     1201 gaagccggtg tacgacaggc ccccggagat gcagatcgac cgatcgaaga cctacaccgc
     1261 caccatggtc accacctgcg ggacgatcag gctccgcctc gacgcgaaag gaagcccgca
     1321 gaccgtcaac agcctggtgt tcctggcccg gaagcgcttc ttcgacggcc tgaagtttca
     1381 ccgcatcgtc aagaacttcg tgatccaggg tggggacccc aagggcgacg ggaccggcgg
     1441 acccggctac tccaccgtcg acacgccgcc gaagggcctg aagtacgagg tgggcgacct
     1501 ggccatggcg aagacgcagg ccgaggcgcc ggggacggcg gggagccagt tcttcatcgt
     1561 cacgggcgac ccggcgccgc tgaacgcctc gccggtgtac gccctggtcg gcacggtggt
     1621 ggaggggctt gacgtggcgc agacgatcga gcagctgccc gcggtcggcg gggccaccga
     1681 tggcgtcccc gccgacgccg tgtacatcga gtcggtcacg atcgaggaac gttgagcgcg
     1741 gggggtctgg gggggtagct cgccctggga gcgacaccgc ccgagcgcgt ccggcgagcg
     1801 aaccccccag ggggaacgcc gtctgcgcgg atggggcgcg cgctgtacgt gctgccgcgg
     1861 gcgcagcggc gcggggcggc gatgacctcc acccgcctcg cggcgcagct tcgcggggaa
     1921 tgggagccgc tcgtcgtcac gctcttcacc ggtggcgacc cctccgtcct gggcgcggcg
     1981 gtaccgatcc gccagctcga ggttcgcgcc gggctggggc ggcgggtgct cggcgtgtcg
     2041 ccggcggcgg tccgcgcgct gcgcgcggcc gccctcgagt tccgcccgga cctgctggtc
     2101 gcctggggcg gggagccgct gctgcacgcg gcgccggttg cgcgccgggc cggagcgcgt
     2161 ctgctctacg tgaagatctc cccgacccag ccgaagctgg tgcgcggcct acgccggatc
     2221 gtgttccgcc gcgcgctggc ccgcgccgat tggctggtgg ccgtgacggt ggagctcgcg
     2281 gaggagctgc gccgcgacct cggcgtgcgc cgcccggtgg ccgtggctca caacttccgc
     2341 ctgccgaccg accggccgtc gccgggtgac cgggcgtcgt tccgcgcgga gctcggcgtg
     2401 ggcgacgacg tggtcctggc ggcgttcgcc gggagcctgt cccgcgacaa ggggcccgac
     2461 caggcgctcg aggcgcttgc tctggcgacg gggaacgtgc acctcgccgt ggccggggcg
     2521 ggaccgctcg agggagcgct gcgcgagcgc gccgcgcgcc cggacctggc cgggcgggtg
     2581 cacctgctcg ggtcgcgcgg cgacgtcccg cgcatcctgg ccggcgccga cctggtcctc
     2641 ctctcgtcgc gggaggaggg ccggcccggg gtgctgctcg aggccgcgct ggccggccgg
     2701 ccggcggtcg ccttcgccga agggggtgtg gcggctctgg tggacgacgg cgtggagggc
     2761 gtgctggtgg cgcccgggga cgtcgcggcg ctcgggcggg cgctgtcggc gctggcggcg
     2821 gacccggcgc ggaggcaggc gatgggatcg gcggccgcgc gccgcggcga cgggtacctg
     2881 gcgccggcgg ccctccccgc gtggcgcgag gcgttccgcg ccgctacagc aggacgacgt
     2941 tcttcggcgc cgcgctcttc ggagccaggc cccgggtgac cccccgcgcg tcgaacacca
     3001 gctgggatcg cgccaccacg tcggcgtagt ccaccgcgct gtgcggcgtc aggatcacga
     3061 cgcagtccgc gcgttccagg gccgccggcg tgagcttcac ggacgagagc ttgcgggtcc
     3121 cgatccggat gtccggcacg aacgggtcgt ggtaggagat cgtggcgccc gacttcagca
     3181 gccgctcgac gaccaccacc gccggcgact cgcggatgtc gttgacgtcg gctttgtagg
     3241 tcagcccgag gacgaggacc ttcgccttgc gcagggcctt gccgtgcgcg ttgagcgcct
     3301 cgccgatccg gccggccacg taggccggca tcttcgcgtt gacttcctgg gcgtgctcga
     3361 tgaaccgcgg ccggtgcccc agccgctgcc cgacccgcca cgacaggtag gagggatcga
     3421 tcgcgatgca gtgtccgccg acgcccgggc cgggccagaa cgccatgtac ccgaacggct
     3481 tggttgcggc cgcgccgatc gcctcccaga tgtcgacgcc gaggtcgcgc gacagcatcg
     3541 ccagctcgtt catcagcccg atgttcacgt ggcggaaggt gttctcgatg atcttggcca
     3601 tctcggcctc gcggggggag cgcaccgtga tcacctcgtt caccagcgtc cggtagaagg
     3661 cggccgcgac ctcggtcgag cgcggcccga tgccccccac cacgcgcggg gtggtgacca
     3721 cgtgggcggt ctcgcggccc ggatcgatcc gctccggcgc gtacgccagc gcgaagtcga
     3781 cccccgccac caggccggag gtctcgagga tcgggcgcag ctcctcctcg gtggtgccgg
     3841 gataggtggt cgactcgagc acgacgaggg tgccgggccg gaggtgcttc gcgaccttcg
     3901 tcgcggcggc gcggacgaac gagaggtccg gctcgtggtc ggcgagcggc gtggggacgc
     3961 agatgaccgt cacgtccgcc ccggcgagca ggcc
//
LOCUS       DASPWO010000035         8361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_516313, whole genome shotgun sequence.
ACCESSION   DASPWO010000035 DASPWO010000000
VERSION     DASPWO010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516313"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VEO00_00805"
     CDS             <1..517
                     /locus_tag="VEO00_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HYU56573.1"
                     /translation="IRGLVADGTTVLLTTQYLEEADQLADEIAVIDRGRVVATGTSNE
                     LKAQVGGQILQVRPVREADRFTVARVVADLTGVEPEVENGVVTAAVPDPAVPPAVFRQ
                     LTEAGVLVAEFSLRRPSLDEVFFALTGHHASTEDAGDDTRGDTGGGGHTADDNHTDDT
                     DDDNDSERSAA"
     gene            514..1332
                     /locus_tag="VEO00_00810"
     CDS             514..1332
                     /locus_tag="VEO00_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU56574.1"
                     /translation="MSAIVIAARPEAEAISSGVGPGQALRNVLTIAWRSLVAIKHSPM
                     ELVDLSVQPLMFVVLFAYVFGGAVAGSPQAYLEFGLAGIIVQNAMFLTLNTAMGLNND
                     LSKGVFDRFRSLPIARSAPLAGRIAADVVRQLWGISILLGVGMLLGFRIHTGVPEVLL
                     AVGLVLVFVLAFSWLPVLVGVLVNSPEKVQVFGFVLIFPLTFASGAFVQVDTMPGWVQ
                     AWVKANPVTLMSDALRGLLVGGGYGRPALQALAWAAAIAAVFAPLAARALRRRA"
     gene            complement(1568..4786)
                     /locus_tag="VEO00_00815"
                     /pseudo
     CDS             complement(1568..4786)
                     /locus_tag="VEO00_00815"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
     gene            4814..5890
                     /locus_tag="VEO00_00820"
     CDS             4814..5890
                     /locus_tag="VEO00_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HYU56575.1"
                     /translation="MLRRAFSAVPAICLLALAAIAPAAAGAANAYHVTKLVSDQPGVA
                     AHMDPNLVNAWGLVAGPSTPWWVADNETDLSTLYTGDGDPLPLVVNVPGGPTGIVFNG
                     GSGFVVNDGTNSGAARFVFDTEAGTIRGWSPGVPPPPLSTQTEVGADRSGVGAIYKGL
                     AIASTDDGDRLYAADFHNARVDVFDGDFGLVTMPGAFVDPKLPPRFAPFGIQTIGGKI
                     FVAFAKQDPDAEDEVAGRGLGFVDAFDTDGTLLRRVATRGPLNAPWGLAMAPAGFGRF
                     GGDLLVGNFGNGQINAYVERPNGSFEHDGRLRVPNGRPLAIDGLWALQFGNDSAAGSA
                     DSLFFTAGPDDETHGLFGEVDPAP"
     gene            complement(5896..7398)
                     /locus_tag="VEO00_00825"
     CDS             complement(5896..7398)
                     /locus_tag="VEO00_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56576.1"
                     /translation="MRGGLLGPVHGVRGDRAGRIAPTCSFDAGTHVLSVTSAGFDVLF
                     AKDGTNLSVSGTDCAALNNVDTVNVDMQSNDRQVGFNLQNGLLEPGFTNEGDASSEIE
                     FDLTNVSTLGEVFVTGGSGPDRFTAGDRKIGQQTVLAINLNGNHDGFSPDEDVIVHTA
                     LVALGLDGGNGDDFLSSHGTGATGSSPAVAWTTFSDGPGADEVAGGDGNDRFFPQVTA
                     DEGDSFSGGDGFDWLILGSRSEQMSLTEDGVRNDGIQCPGLGCEDDFVAADSDRISGG
                     SGPDVIVGGAGHQVLDGGMGNNSISGGPGNDLLTAGTDTDVFHGGAGFDTVSYEHHFS
                     SGVNVFLNGNADDGIPGEHDNVMPDVEGIYGSGKSDLLVGDAARNILDGGPGDDVLNG
                     KSGNDTLVDATSPPVDGGFSIFTGGDLFIGGPGKDTVDERGHNGNLTLTIDGNSNDQV
                     QGDASQGVDNIQTDVENLIADDGDDTLNGGPGTDTCKQGPGTGPKTGCEH"
     gene            complement(7554..8045)
                     /locus_tag="VEO00_00830"
     CDS             complement(7554..8045)
                     /locus_tag="VEO00_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56577.1"
                     /translation="MRKMVIVVTALALVVGVAWGVLASTGSGTTPISCMDSQWQTTAQ
                     STSSTEWTNVPGFTDNPSQVFPIAINVSAQLSGAPVLFRIRATNVGEQTFTSHPGPTR
                     FVPGTNGPNSFAYQWIDRGNAASEHVLTVRLQWRSPTGDAVHMQRGDMSLLYATDGCQ
                     GAP"
BASE COUNT         1114 a         3094 c         2950 g         1203 t
ORIGIN      
        1 catccgtggc ctggtggcgg acggcacgac ggtcctcctc accacgcagt acctggagga
       61 ggccgaccag ctggccgacg agatcgcggt gatcgaccgg ggccgcgtgg tggccaccgg
      121 cacctcgaac gagctcaagg cgcaggtcgg cgggcagatc cttcaggtga gaccggtccg
      181 ggaggccgat cggttcacgg tggcgcgggt ggtcgccgac ctgaccggag tggaaccgga
      241 ggtcgagaac ggcgtcgtga cggcggcggt ccccgacccg gcggtgcccc cggcggtctt
      301 tcggcaactg accgaggccg gcgtgttggt ggcggagttc tccctgcgga ggccgagcct
      361 ggatgaggtc ttcttcgccc tcaccgggca ccacgcgagc acggaagacg cgggggacga
      421 cacccgcggc gacacgggcg gcggcggcca cacggcagac gacaaccaca ccgacgacac
      481 cgacgacgac aacgacagcg aaaggagcgc ggcatgagcg ccatcgtcat cgccgcccgc
      541 cctgaggcgg aggccatcag cagcggggtc ggccccggcc aggccctccg caacgtcctc
      601 acgatcgcgt ggagaagcct ggtcgcgatc aagcacagcc ccatggagct ggtcgacctg
      661 agcgtccagc cgctgatgtt cgtggtgctg ttcgcctacg tgttcggcgg ggccgtggcc
      721 ggctcgcccc aggcgtacct cgagttcggg ctcgccggga tcatcgtgca gaacgcgatg
      781 ttcctcacgt tgaacaccgc gatggggctg aacaacgacc tcagcaaggg cgtgttcgac
      841 cggttccgga gcctgccgat cgctcgctcc gcgccgctcg ccgggcggat cgccgccgac
      901 gtggtccgcc agctgtgggg gatctccatc ctgctgggcg tggggatgct cctcgggttc
      961 cgcatccaca cgggcgtccc cgaggtgctg ctcgcggtgg gcctggtcct ggtgttcgtc
     1021 ctcgcgttct cctggctccc ggtgctcgtc ggagtcctgg tgaactcccc cgagaaggtc
     1081 caggtgttcg ggttcgtcct gatcttcccg ctgaccttcg cgagcggggc gttcgtccag
     1141 gtcgacacca tgcccggctg ggtgcaggcg tgggtgaagg cgaacccggt cacgctgatg
     1201 tcggacgcgc tccggggtct gctggtgggc ggcggctacg gccggccggc gcttcaggcg
     1261 ctcgcgtggg ccgcggcgat cgcggcggtg ttcgccccgc tcgctgcccg ggccctccgc
     1321 cggcgggcgt aggggcggct gctggagggg cctcggcgcc ggcctcggcc cgaggcctct
     1381 cggcctgtgg ggcctgagga tcctcggcct ctgctccgag cgcctcgagc acctccgcgc
     1441 gcgaccgcgc ggcgccgcgc cggtaggccc ggtcgaatcc cgggtcaccg agcgcggccc
     1501 gcgcgccggc gcggaccctc cggacctcgg ggtcggcctg gtcggccacc cctcgcacca
     1561 cctcggccgt cccgaggagc gtagccgcaa gccccgcgtc cccggccgcg agcgcgacgt
     1621 ccgcgcggag ctccacggtg cgggcgacga tcggatagtc gcgggttccg agcgccaccc
     1681 gcagcgccca ccggtaccag gcgacggcct cctccaactc gcccgcggtc acctccaccc
     1741 gcccccgcgt caccagcagc atcgcgtgga gctggtcgat gggcttgccg aacccctgga
     1801 agtcggcgat cgcgccgtcc agcatctgcc tcgcgtcgtc caggcggccc gacgtcctgg
     1861 cggcctcggc ccgggcctgc cggatccaca gcttctggtc ctccgccccg atctcctcgg
     1921 cgatccgctc ggccgcgtcc agctcgtcga gggcacgttc gaactccccc gcctgcgccc
     1981 gctgggccgc ctcccgaatc atcagctggg cgatgtcctc gcggtcgtcg agcaggcgga
     2041 acacctcctg ggcctcccgg agcgctgcgg cggcggcctc ctggtcgccc cgggtcgcgc
     2101 gaaggtcggc gaacgccacc agcgagttcc cgagccccca gtggtccccc agcttccgga
     2161 actgcgcgac cgccgtgtcg atctcgtcgc tcgcctgttc gacctcgccc atgttgatga
     2221 gcagctgccc cgcgaccagg tggcccagcc cgcggaccca caggtctggc gagtcgaact
     2281 gggcacgcgc gagcgcgagc gcgcgctcgt cctcgccgcg gaacatcgcc atcagcgtct
     2341 ccccgaactc gagcgtcggg aaccgcacga gttcctcggg cgggagcgtc cggatgatgg
     2401 tcaccgcctc gtcccccagg cggagcgcaa gctcgaactc cgacccgccg gtgacgccga
     2461 ggagggagcg gatgagcagg cccccggcgc ggatcgtggg cggcacgtcg ccgggcaggg
     2521 ccagcgcgcg gtccgcccac tcgagcgcct ccgccctgga gccccgcatg aaccagtacc
     2581 acccgagcag ggcgaccagc cgcatcgcca gggctgcgtc ccccgcatcg accgcaaacc
     2641 ggagggccgc gtggatgttg tcgcgttcgg cccacagccg cgccagccac ttcacctgtt
     2701 ccggcccgcg gaggtggggg tcggccgccg ccccgaagtc ggccaggaac tcggcgtgcg
     2761 cccgccggag aacctcgtcc tcgccggcct cgcgccgccg ttcgtcggcg taggcccgga
     2821 tggtgtccag catccggtat cggacctcgt tcgtcccgtc gtcccggacc gagaccacca
     2881 atgacttctc caccagggag gcgaggaggt acagcacctc ctcccggggc ggcccatcca
     2941 gctcgccgcc tgcgcagacc gcctcggccg cctcgaggtt ggcgccggcc gggaacaccg
     3001 ccagcctccg aaggagcatg cgctccggct ccgtgaggag gtcccagctc cagtcgacga
     3061 cggcccgcag cgtcttgtgc ctggggaggg ccgccccgcc tccgactccc agcaggcgga
     3121 agcggtcgtc caggcggtcc gcgatctggc cggtggtgag gctccggagc cgggccgcgg
     3181 ccagctcgat cgcgagcggc atgccgtcca gcgcgcggca gatcgcgacc acgtcgcgca
     3241 cggtgctctc atcgacctcg aatgccggct gcaccgactc cgcccggtcg gcgaacaggc
     3301 ggaccgcggc ggagcgcttc gctgtggcgg gcgcgatccc ggcgccgtcc ttcggcatcg
     3361 ccaggggctc caccggaacg agcacctcgc cggtgatccc cagcggctcg cggctggtcg
     3421 cgaggacgtg gagtgacggg caccgtccga gcaggtggtc ggtgagggcg gccacggccg
     3481 agatgaggtg ctcgcagttg tcgacgagga gtaggagatg ccggtcgccg atggcctcgg
     3541 tcagccgttc gcgtgggctc acggcgccgg cgccggcgcg ggccagcagg ccggtctccc
     3601 tggcgccgaa caccgacagg aaggcctggg gcagctcgcc ggggtcaccg acgggagcga
     3661 gctcgatcat ccacaccccg cccgggaccc ggtccaccag gcccgccccc gcctcgctgg
     3721 ccaggcgagt cttgcctgac ccgccgggcc cgaccagcgt caccagtcgc gagtcggcca
     3781 ataccgacgc gatccgctcg acgtcgcgct cgcggccgac gaagctcgtg atctgggacc
     3841 ggaggttggt ccgggcggcc ggcgcccgct cgcgctgttc ccccccggct ggtcccgagg
     3901 gagccggcgc accctccgga cgttcggact cgccacgaag caccgcgagc tggatcgctc
     3961 ggagctcctc cgaggggtcg atgccgagct cgtcggccag ccgcgctcgc agccgttcga
     4021 acgcggcgag ggcgtcggcc tgccgcccgg tcgcggcgag cgcccggacg agctgcccgt
     4081 tcaggcgttc gcgcaggggg tggccggcga ccagcgcctc gagctcggcg accaccgtgc
     4141 cgtcgccccc ggcggcgagg tcggcctcga tgcggtcctc gagggcgccg agccgcagct
     4201 cctccagccg ggccgccgcc gccacagcga acccagcgtc agccgcgtcc gccagcgcgg
     4261 gcccccgcca cagcgccagc gcctcgcgaa ggtccgacgc cgcccccgcg ggatcatcgc
     4321 ccagccgccg gcgcccgccc gcggcgagtg cctcgaaccg aaccgcgtcg acggcgtcgt
     4381 ccggcacgac cagccggtag ccggtcggat gcgtctcgat gagcccgggc agcgcgcggc
     4441 gcaccctcgc caccagcgac tggagtgcgt tggtggctcc gtcgggcggc tcctcgcccc
     4501 agaccgcgtc gatgagctgg gacgtcagca ccacgcgccc cggatcgagc gccaggcgga
     4561 tcaggagcgt ccgcagccgc acgccggcga ggggcgcagc ttcgcggccg agccgcacct
     4621 cgagcggccc cagcacccgg atctccacgc tcccagccac cggaccattg tggcggcgct
     4681 cgcggacagc gtcgccccgg ccggattcgt tcgagccggg gcgggggctc ggccgtagta
     4741 cctgggaagg ccccactggc cgcgacggcc gccgcgccgt cgccacatcc cctgcgcccg
     4801 aaggaggtca tccatgctca gacgagcgtt ctcagccgtg cccgcgatct gtctgctggc
     4861 actcgccgcg atcgcccccg ccgcggcggg ggcggcgaac gcctaccacg tgacgaagct
     4921 cgtctccgac cagccggggg tggccgcgca catggacccg aacctggtca acgcgtgggg
     4981 gctcgtggcg ggcccgtcca cgccatggtg ggtcgccgac aacgagactg acctctccac
     5041 cctgtacacc ggtgacgggg atcccctacc actcgtagtg aacgtgcccg gtggaccgac
     5101 cggaatcgtg ttcaacggcg gctccgggtt cgtggtgaac gacgggacga actccggcgc
     5161 ggcgcggttc gtgttcgaca ccgaggccgg caccatccgc ggatggagtc cgggcgtgcc
     5221 tcccccgccg ctctcgactc agaccgaggt cggcgccgac cgttccggcg tcggtgccat
     5281 ctacaaggga ctggccatcg cctcgaccga cgacggcgac cgtctctacg cggccgactt
     5341 ccacaacgcc cgggtcgacg tgttcgacgg cgatttcggg ctcgtcacca tgcccggggc
     5401 cttcgtcgac cccaagctcc cgccgcggtt cgcgccgttc gggatccaga ccatcggcgg
     5461 caagatcttc gtggccttcg ccaagcagga ccccgacgcg gaggacgagg ttgccggccg
     5521 cgggcttggc ttcgtggacg cgttcgacac ggacggcacg ctcctccggc gcgtggctac
     5581 ccgggggccg ctgaacgccc cgtggggcct ggccatggcc ccggctggct tcggccggtt
     5641 cggtggcgac ctgctcgtcg ggaacttcgg caacggccag atcaacgcgt acgtcgaacg
     5701 cccgaacggc tcgttcgagc acgacgggag gctgcgggtg ccgaacggcc ggccgctcgc
     5761 gatcgacggc ctgtgggcgc tccagttcgg caacgacagc gccgccgggt cggcggactc
     5821 gctgttcttc acggcgggac cggatgacga gacgcacggc ctgttcgggg aggtcgaccc
     5881 ggccccgtaa gcggctcagt gctcgcagcc ggtcttcggg ccggtgccgg ggccctgttt
     5941 gcaggtgtcg gtgccggggc cgccgttgag ggtgtcgtcg ccgtcgtccg cgatgaggtt
     6001 ttcgacgtcg gtctggatgt tgtccacgcc ctgggaggcg tcgccctgga cctggtcgtt
     6061 cgagttgccg tcgatggtga gcgtgaggtt gccgttgtgc ccgcgctcgt ccacggtgtc
     6121 cttgcccggc ccaccgatga acaggtcgcc gccggtgaag atgctgaagc ccccgtcgac
     6181 cggtgggctc gtcgcgtcga ccagcgtgtc gttgccgctc ttgccgttca ggacgtcgtc
     6241 gccgggaccg ccgtccagga tgttccgggc tgcgtcgccg accagcaggt cgctcttccc
     6301 cgagccgtag attccctcga cgtccggcat cacgttgtcg tgctcaccgg ggatgccatc
     6361 gtcggcgttg ccgttgagga agacgttgac gccgctcgag aagtgatgct cgtacgagac
     6421 cgtgtcgaac ccggctccac cgtggaacac gtcggtgtcc gtgccggctg tgagcaggtc
     6481 gttgcccggc cccccgctga tcgagttgtt gcccatgccc ccgtcgagga cctggtgccc
     6541 ggcacccccg acgatgacgt ccggccccga ccccccggag atcctgtcgc tgtcggcggc
     6601 cacgaagtcg tcctcgcatc caaggccggg gcactggatc ccgtcgttgc gcaccccgtc
     6661 ctccgtgagc gacatctgct ccgagcgcga gccgaggatc agccagtcga acccgtcccc
     6721 gcccgagaac gagtccccct catccgccgt gacctgcggg aagaagcggt cgttgccatc
     6781 gccgccggcc acctcgtccg ccccggggcc gtcgctgaag gtcgtccagg ccacggccgg
     6841 cgacgagccc gtggcaccgg ttccgtggga gctcaggaag tcgtctccgt tgcccccatc
     6901 gaggccgagg gccaccaggg ccgtgtgcac gatcacgtcc tcgtccgggc tgaagccgtc
     6961 gtggttcccg ttgaggttga tggccagaac ggtctgctgc ccgatcttgc ggtcgcccgc
     7021 cgtgaacctg tccgggccgg atcccccggt cacgaacact tcgccgagcg tggacacgtt
     7081 cgtgaggtcg aactcgatct cactcgacgc gtccccctcg ttcgtgaatc cgggctcgag
     7141 cagcccgttc tggaggttga accctacctg ccggtcgttg ctctgcatgt cgacgttcac
     7201 ggtgtccacg ttgttcaggg cggcgcaatc ggtgccgctc accgacaggt tcgtcccgtc
     7261 cttcgcgaac agcacgtcga agccggccga cgtgacgctg aggacgtgcg tgcccgcgtc
     7321 gaacgagcac gtcggggcga tgcggccagc gcggtcccca cgaacaccgt ggacaggacc
     7381 caggaggccg ccgcgcacgc cagtagcaac cgcgggatgg cgggcttcga ggctctgaag
     7441 ctggggcgca gcatggaggg ccatagagac cccgagacac cggccccgtc aagtccactg
     7501 ttcggcggct gggcgccggg cggggaaacg gcgtcccggc cggcgccgcg accctatggc
     7561 gcgccctggc agccgtccgt cgcgtagagc agcgacatgt cgccgcgctg catgtgcacg
     7621 gcgtcgccgg tggggctccg ccactgcagc cggacggtga ggacgtgctc cgaggcggcg
     7681 ttcccgcggt cgatccactg gtaggcgaac gagttggggc cgttcgtgcc gggaacgaac
     7741 cgggtgggac cgggatggct cgtgaaggtc tgctcgccga cgttcgtggc gcggatccgg
     7801 aacaggacgg gcgctcccga gagctgcgca ctcacgttga tcgcgatcgg gaagacctgc
     7861 gacggattgt cggtgaaccc cgggacgttc gtccactcgg tggaggacgt cgactgggcc
     7921 gtcgtctgcc actgactgtc catgcagctg atcggggtcg ttccgctccc cgtgctcgcc
     7981 aggacccccc aggcgacccc gaccacgagg gccagggccg tcaccacgat caccattttc
     8041 cgcatggcgg cctccttcca gaggactcgg tccgccggac gctatgaccg gtgtccggat
     8101 tccgcaaacg gcgggccgat cggcccgtcg gagaacaagg acgcgggggt ttggcgggag
     8161 gttgcaacgg cggatcggcg aggtatcggc cgtgagccgg gtgaagctcg tggccacgcc
     8221 cgaaccgggc cttcacgatc gccgtcgacc tcgacgtcac ggtgctccca gccggcgcag
     8281 cccgcccgcg aaccgcgcca cacggcaacg acctcctcga cggcggggga tcgctgacct
     8341 tcgatccccg ggcagtcgac g
//
LOCUS       DASPWO010000036         8098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_89242, whole genome shotgun sequence.
ACCESSION   DASPWO010000036 DASPWO010000000
VERSION     DASPWO010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89242"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1429)
                     /locus_tag="VEO00_00835"
     CDS             complement(128..1429)
                     /locus_tag="VEO00_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HYU56578.1"
                     /translation="MTHSATERTLRTPALARVEGEGAMVLRLRDGGVEEVQLNIYEPP
                     RFFEAFLVGRRYTEPPDITARICGICPVAYQMSACAAIEDACAVEVTGPLRDLRRLIY
                     CGEWIESHALHVYLLHAPDFLGYPSALELAADHRAVVERGLQLKKAGNEVIELIGGRA
                     IHPINVRVGGFYRAPTAAELRPLRERMERARDAALETVRWVAGFDFPDFDQDYEFVAI
                     HGDGEYPMLGDRVASSGGLEFGVRDFEEHVVEEHVPHSTALHARLDGARPYLTGPLAR
                     YALNRELLSPAADAAAREAGLGDEVRNPFRSIVVRAVEIVHACEEAIRIIDGYEPPDH
                     PFVDAPPRAATGFGCSEAPRGVLFHRYAIDGDGLIASARIVPPTSQNQGQIEDDLRRF
                     VEPRVELPVEELTAQCEQAVRNHDPCISCATHFLDVTVEQA"
     gene            complement(1419..2189)
                     /locus_tag="VEO00_00840"
     CDS             complement(1419..2189)
                     /locus_tag="VEO00_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HYU56579.1"
                     /translation="MGRPTVAVWKFASCDGCQLSLLDCEDELLQVAEGVRIAYFLEAS
                     KATVEGPYDLSLVEGSVTTAHDAERIREVRAASKALVTIGACATAGGIQALRNFADVE
                     AFTNLVYARPEYISTLATSTAIADNVKVDFELRGCPIDRGQLLEVISAFLHGRKPGVP
                     SHSVCVECKLRGTVCVMVAKGTPCLGPVTHAGCGALCPAYDRGCYGCFGPMETPNTPS
                     LAGWLGGLGMGEGDLVRVFRTFNANAPGFREESEAHDA"
     gene            complement(2190..2996)
                     /locus_tag="VEO00_00845"
     CDS             complement(2190..2996)
                     /locus_tag="VEO00_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HYU56580.1"
                     /translation="MVPRRYRVAARTVETADTATLTLEPAGEPIGPAAPGQFNMLYAF
                     GIGEVPISFSGLDGHGGPPVHTVRAVGAVSRALWGAEVGATLGVRGPYGTRWDLEAAA
                     GGDVVLMAGGIGLAPLRPVVHRLLAERDRYGRVSVLVGARSPDLLLFPAELVAWSGKV
                     RVATTVDHGDASWRGNVGVVTEVLREAEFDPARTAAFVCGPEVMMRFSARALADAGVD
                     PARIQVSLERNMKCAIVHCGHCQLGPEFICGGGPVFPFKRAERLMSVREL"
     gene            complement(3016..3495)
                     /locus_tag="VEO00_00850"
     CDS             complement(3016..3495)
                     /locus_tag="VEO00_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYU56581.1"
                     /translation="MNIQSIDELLAHHPFFAGLDPKDLLFMRVAGCGRNVHFPEGMYI
                     LREGEPADRFYIVREGRVALEIAAPSRGPIVLDTLGGGAVVGASWLFPPYRWRFDVRA
                     VEPTSAVALDAVCLREKCEEDPRLGYELMKRMSGVLEERLQSARMRLIDLYSHAGTG"
     gene            complement(3492..4637)
                     /locus_tag="VEO00_00855"
     CDS             complement(3492..4637)
                     /locus_tag="VEO00_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYU56582.1"
                     /translation="MDESAVIDLSELDALIATLRAREYRVIGPVARDGAIVYDEVASA
                     AELPVGWTDEQDGGTYRLRPRGDEARFGYAVGPHSWKKFLFPPRSILWRAHRTDAGFE
                     VEEPPDEAPPFAFLGVRSCELHAIAIQDKVFLETKHPDPTYAGRRERAFIVAVNCGTP
                     AGTCFCVSMNTGPRASGGYDLALTEVLGNGSPEYVIEVGSDRGAEVLAELPRRPATAG
                     DANAAVAVTDEAARNMGRQLDTTGIHGLLQSNPEHSRWDDVASRCLACGNCTLVCPTC
                     FCNTVEDTTDLTGEHAERSRRWDSCYTLDFSHLGEGSVRTSTRSRYRQWLTHKVDTWF
                     DQFGVSGCVGCGRCITWCPVGIDLTEEIPAIRAPDGRAGPEHEGGRP"
     gene            complement(4746..5252)
                     /locus_tag="VEO00_00860"
     CDS             complement(4746..5252)
                     /locus_tag="VEO00_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYU56583.1"
                     /translation="MTIERNPHEEVDRLLGTVDEVMERTVLVLAPGATLDEAAHEMER
                     RGVSGAPVVEGGRVVGIVSLSDLFEIAGISAAQARTASPWRRYERRLSESGKTVRSAM
                     TSAVYTLGPGASVAHAAELMWEHGVNRVPIVGTDGQVEGIVAREDVVAAVARAGLGLR
                     VRPPVLQP"
     gene            5413..5703
                     /locus_tag="VEO00_00865"
     CDS             5413..5703
                     /locus_tag="VEO00_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56584.1"
                     /translation="MDRPVRALIVSRSLAVRGAWARWLRTAGIDPALCHGPAPGRPCP
                     RANGDTCPLREWADVAIVDPDGTAAAFGLHSLADLGEICTRVPDDGTTLVLP"
     gene            complement(5752..6273)
                     /locus_tag="VEO00_00870"
     CDS             complement(5752..6273)
                     /locus_tag="VEO00_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HYU56585.1"
                     /translation="MVQASGLLDPAVAEEEQNDKLVIIAWSGELDKMWPTLILATTSA
                     AMGQETTVFFTFWGLFPLVKNDVRITGENWMQKMMSFMNRGGTDHLKLSKFHFAGAGK
                     AMMMKLARDHKVASPTELMEMARDMGVHLVPCQMTMDLLGISREDLIDGVEEPIGATT
                     ALLEAKGATTFFI"
     gene            complement(6279..6512)
                     /locus_tag="VEO00_00875"
     CDS             complement(6279..6512)
                     /locus_tag="VEO00_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HYU56586.1"
                     /translation="MSQTITTVDARGLSCPLPVVKAAQALKLLGPGETFEVLATDPGS
                     QSDIPAWCRATGTELVEHGEDGGVYRYLLKKKG"
     gene            complement(6516..7700)
                     /locus_tag="VEO00_00880"
     CDS             complement(6516..7700)
                     /locus_tag="VEO00_00880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HYU56587.1"
                     /translation="MPARIVVLGGGVGGTLAANLLHKELGRNATVTVVDPTGMHVYQP
                     GFLYVALGQANGRWLSRDERTLLYPGVELAIEGAARVHPDRGEVELQRGGSLPYDYLV
                     MATGARLVPEQVPGMAEGAQHFYSLEGALRLREELDRFDGGKVVVGVAGIPYKCPPAP
                     VEFVFMLEEYLRDRGIRGRSEVTLLSPLNRAFTIESASKVVQPIMESRGIGLSTFFNV
                     ETVEPAAGVVTSLEGEKAEYDLAVIVPPHRGQSVVEASGLGDPGGWIPTDRTTLQVEG
                     QERIFAMGDATNLPISKSGSTAHFEAPVIASRMASLIRGTAPKEGYEGRVMCFLETGN
                     RRTTVLRFDYEHPPIPPKPSLHWHVAKWLFNRIYWATVPKGRVPDIGTRPKAVPEKKK
                     EG"
     gene            complement(7705..7944)
                     /locus_tag="VEO00_00885"
     CDS             complement(7705..7944)
                     /locus_tag="VEO00_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HYU56588.1"
                     /translation="MTTIDTTQVIDARGMACPGPLMSLIGAIREGAVGDVIEVLSSDE
                     GSKTDIPAWVAKAGHELVEVAPEDGFARFVIRKAR"
BASE COUNT         1124 a         3060 c         2764 g         1150 t
ORIGIN      
        1 tcaccgtcgt gctcgtgcac cgtgacgccg gccggggcca ccgcggccac gcgccgggcg
       61 acctcgagcc cggccgcgtc gtcgccgcgg taggggttcc cgacgccgat cacggtcacc
      121 gccgccctca cgcttgctcc acggtcacgt ccaggaagtg ggtggcgcac gagatgcagg
      181 ggtcgtggtt tcgcaccgcc tgctcgcact gcgcggtcaa ttcctcgacg ggaagctcga
      241 cccgcggctc gacgaaccgg cgcaggtcgt cctcgatctg cccctggttc tgcgacgtcg
      301 gcggcacgat ccgtgcggag gcgatcagcc cgtcgccatc gatcgcgtag cggtggaaca
      361 ggaccccgcg cggggcctcg ctgcagccga agccggtggc ggcccgcggg ggcgcgtcga
      421 cgaacgggtg atccggcggc tcgtacccgt cgatgatccg gatggcctcc tcgcaggcgt
      481 gcacgatctc cacggcgcgg accacgatgc tccggaacgg gttgcgaacc tcgtcgccga
      541 gccccgcctc ccgcgccgcg gcgtctgccg ccggcgagag cagttcccgg ttcagcgcgt
      601 accgcgccag cggcccggtc agatacgggc gggccccgtc caggcgcgcg tgcagcgcgg
      661 tcgaatgtgg gacgtgctcc tcgacgacgt gctcctcgaa gtcccgcacg ccgaactcga
      721 gcccgccgct cgacgccacg cggtcgccga gcatcgggta ctcaccgtcg ccgtggatcg
      781 ccacgaactc gtagtcctgg tcgaagtcgg ggaagtcgaa gccggccacc cagcgcacgg
      841 tctcgagggc ggcgtcgcgc gcccgttcca tgcgctcgcg gagcgggcgc agctccgccg
      901 ccgtgggcgc ccggtagaag ccgccgaccc ggacgttgat cgggtggatc gctcgcccgc
      961 cgatcagctc gatgacctcg ttcccggcct tcttcagctg caggccgcgc tcgacgaccg
     1021 cgcgatggtc ggcggccagc tccagcgcac tcgggtaccc caggaagtcg ggcgcgtgca
     1081 gcaggtagac gtgcagcgcg tggctctcga tccactcccc gcagtagatc agccggcgaa
     1141 ggtcgcgcag cggcccggtc acctccacgg cgcaggcgtc ctcgatcgcc gcgcacgcgc
     1201 tcatctggta ggcgaccgga cagatcccgc agatccgcgc ggtgatgtcg ggcggctccg
     1261 tgtagcgccg gccgaccagg aaggcctcga agaaccgcgg cggctcgtag atgttgagct
     1321 gcacctcctc gaccccgccg tcgcgcaggc gcaggaccat cgccccctcg ccctccaccc
     1381 gggccagggc gggcgtgcgc agcgtccgtt cggtggcgct atgcgtcatg ggcctcgctc
     1441 tcctcacgaa agcccggggc gttcgcgttg aacgtccgga acacgcgcac caggtcgccc
     1501 tcccccatcc ccaggcctcc cagccagccg gccagcgacg gcgtgttcgg cgtctccatc
     1561 ggcccgaagc agccgtagca gccgcggtcg taggcggggc agagcgcgcc gcagccggcg
     1621 tgcgtcaccg ggccgaggca cggcgtcccc ttcgccacca tcacgcacac cgtgccgcgg
     1681 agcttgcact ccacgcagac gctgtgcgac gggacgcccg gcttgcggcc gtgcaggaac
     1741 gccgagatca cctccagcag ctggccgcgg tcgatcgggc agccccgcag ctcgaagtcg
     1801 accttgacgt tgtcggcgat ggcggtggag gtggcgagcg tcgagatgta ctcgggccgg
     1861 gcgtagacca ggttggtgaa cgcctcgacg tcggcgaagt tccgcagtgc ctggatcccg
     1921 ccggccgtcg cgcacgcccc gatcgtgacg agcgccttcg aggcggcgcg gacctcgcgg
     1981 atccgctccg cgtcgtgggc cgtggtgacc gacccctcga ccagcgacag gtcgtagggg
     2041 ccctcgaccg tggccttcga ggcctccagg aagtaggcga tccgcacgcc ctcggcgacc
     2101 tgcaacagct cgtcctcgca gtcgagcagg ctgagctggc atccgtcgca cgaggcgaac
     2161 ttccacacgg cgaccgtcgg ccggcccatc tacagctccc gcaccgacat cagccgttcc
     2221 gcccgcttga aggggaagac cggcccgccc ccgcagatga actcgggccc cagctggcag
     2281 tgcccgcagt ggacgatggc gcacttcatg ttccgctcca gcgacacctg gatccgggcg
     2341 ggatcgacgc ccgcatcggc cagcgcgcgg gccgagaacc gcatcatcac ctccggcccg
     2401 cacacgaacg ccgcggtccg cgccgggtcg aactccgcct cgcgcaggac ctcggtgacc
     2461 accccgacgt tgccgcgcca cgacgcgtcg ccgtggtcga ccgtcgtcgc cacccggacc
     2521 ttgccggacc aggccaccag ctccgccggg aacagcagga ggtccggcga gcgcgccccg
     2581 acgagcaccg agacgcggcc gtagcggtcg cgctcggcga gcagacggtg caccaccggg
     2641 cgcagcgggg cgagcccgat cccaccggcc atcagcacca cgtccccgcc ggccgcggcc
     2701 tccaggtccc accgggtacc gtacgggccg cgcacgccga gcgtggcgcc cacctcggcc
     2761 ccccacagcg cccggctgac ggccccgacc gcgcgcacgg tgtgcacggg cggcccgcca
     2821 tggccgtcca gcccggagaa cgagatcggc acctcgccga tcccgaacgc gtagagcatg
     2881 ttgaactggc cgggcgcggc ggggccgatc ggctcgccgg ccggctcgag cgtcagcgtg
     2941 gcggtgtcgg cggtctcgac cgtccgcgcc gccacgcggt agcgccgcgg gaccatcggg
     3001 ccgggtcggg aggcgtcagc cggtgccggc atggctgtag aggtcgatca gccgcatccg
     3061 ggccgactgc aggcgttcct cgaggacgcc cgacatccgc ttcatcagct cgtagccgag
     3121 gcgagggtcc tcctcgcact tctcgcgcag gcacacggcg tccagggcga ccgcgctcgt
     3181 gggctcgacg gcgcgcacgt cgaagcgcca gcggtagggg gggaagagcc acgaggcgcc
     3241 gaccaccgcg ccaccgccga gcgtgtcgag cacgatcggc ccgcgcgacg gggccgcgat
     3301 ctccagggcc acgcggccct cgcgcacgat gtagaaccgg tcggcgggct cgccttcgcg
     3361 aaggatgtac atgccctcgg ggaagtgcac gttgcgcccg cacccggcga cccgcatgaa
     3421 caacaggtcc ttggggtcga gccccgcgaa gaacgggtga tgggcgagca gctcgtcgat
     3481 gctctggatg ttcacggccg acctccctcg tgctcgggcc cagcccgccc gtccggcgcc
     3541 cggatggccg ggatctcctc ggtcaggtcg atcccgaccg gacaccacgt gatgcaccgg
     3601 ccgcagccta cgcagccgga caccccgaac tggtcgaacc acgtgtcgac cttgtgcgtc
     3661 agccactggc ggtagcggga tcgcgtggac gtgcgcacgc tcccctcgcc gaggtgggag
     3721 aagtcgagcg tgtagcacga gtcccagcgc cgcgatcgct cggcgtgctc gccggtcagg
     3781 tcggtggtgt cctcgaccgt gttgcagaag cacgtcgggc acaccagcgt gcagttcccg
     3841 caggccaggc agcgactggc cacgtcgtcc cagcgggagt gctccgggtt cgactggagc
     3901 agcccgtgga ttcccgttgt gtcgagctgc cgtcccatgt tccgcgcggc ctcgtcggtc
     3961 accgccaccg cggcgttcgc gtcgccggcc gtcgccggcc gccgcggcag ctcggccagc
     4021 acctcggcgc cgcggtcgct gcccacctcg atgacgtact ccggcgaccc gttcccgagg
     4081 acctcggtca gcgccaggtc gtaccccccc gacgcgcgcg ggccggtgtt catcgagacg
     4141 cagaagcagg tgccggccgg ggtcccgcag ttcaccgcga cgatgaaggc gcgctcccgc
     4201 cgcccggcgt aggtcgggtc cgggtgcttc gtctcgagga acaccttgtc ctggatggcg
     4261 atcgcgtgga gctcgcacga gcgcacgccg aggaacgcga agggaggcgc ctcgtccggc
     4321 ggttcctcga cctcgaaccc ggcgtcggtg cggtgggcgc gccagaggat cgaccgcggt
     4381 gggaacagga acttcttcca ggagtggggc ccgaccgcgt agccgaaccg cgcctcgtcg
     4441 ccccgggggc gcaggcggta ggtgccgccg tcctgctcgt cggtccagcc gacggggagc
     4501 tcggcggcgg aggccacctc gtcgtagacg atcgcgccgt cccgggccac cggcccgatc
     4561 acccggtact cgcgggccct cagtgtggcg atcagggcgt cgagttccga gagatcgatg
     4621 actgccgact cgtcgatctc cgccacgctc ccgctcctcc ccccggcagt ctccccagag
     4681 gcgggcgccg acaacacccc acgggggtct ccgccccccg tgtgcccccc gttcgccacc
     4741 ggaacctacg gttggagcac cggcggccgg acccgcaggc cgaggccggc gcgggccacc
     4801 gcggcgacga cgtcctcacg ggcgacgatg ccctcgacct gcccgtccgt cccgacgatc
     4861 gggacgcggt tcaccccgtg ctcccacatc agctcggcgg cgtgcgccac cgaggcgccg
     4921 ggcccgaggg tgtacacggc cgaggtcatc gccgagcgaa cggtcttgcc gctctcggac
     4981 aggcgccgct cgtacctccg ccacgggctg gccgtccggg cctgcgcggc cgagatgccg
     5041 gcgatctcga acaggtcgga caggctgacg atcccgacca cccggccgcc ctccaccacg
     5101 ggagcgccgg acacgccgcg gcgctccatc tcatgggccg cctcgtcgag ggtggcgccg
     5161 ggggcgagca ccagcacggt ccgctccatc acctcgtcca cggtcccgag cagccggtcg
     5221 acctcctcgt gggggttccg ttcgatcgtc acgtcgctcc ctcctcgccc ccagcttccc
     5281 cgaccggcgg cggggccggg aggggcgacc gtcccggctc ggggggtccg ttcgggccgt
     5341 gcagcgcggg acctccgtcc ctgtcctccg tcccgtgcgc gggtagcgtg aggctcgcgg
     5401 gaagggggtt ggatggaccg gccggtgcga gcactgatcg tcagcaggtc gctggccgtc
     5461 cgcggcgcct gggcccgctg gctccgcacg gcggggatcg atcccgcgct gtgccatggt
     5521 cccgcgccgg gccggccctg cccccgagcg aacggcgaca cgtgccccct ccgcgagtgg
     5581 gccgacgtgg ccatcgtcga cccggacggc accgcggccg cgttcggcct gcacagcctg
     5641 gcggacctcg gggagatctg cacccgggtc cccgacgacg gcacgacgct cgtcctgccg
     5701 taggtcgctc ccgcccgcgc ggcgtcccgc gcgaccggcc gttctcgcgc tctagatgaa
     5761 gaaggtggtg gcgcccttgg cctcgagcag ggcggtcgtg gcgccgatcg gctcctcgac
     5821 gccgtcgatc aggtcctccc tcgagattcc caggaggtcc atcgtcatct ggcagggaac
     5881 caggtgcacg cccatgtccc gggccatctc catcagctcg gtggggctgg ccaccttgtg
     5941 gtcgcgcgcc agcttcatca tcatcgcctt gccggctccc gcgaagtgga acttcgacag
     6001 cttcaggtgg tcggtcccgc cccggttcat gaacgacatc atcttctgca tccagttctc
     6061 gccggtgatc cgcacgtcgt tcttgaccag cgggaacagc ccccagaacg tgaagaagac
     6121 ggtggtctcc tgtcccatcg ccgccgacgt ggtggcgagg atcagggtcg gccacatctt
     6181 gtccagctcg ccgctccagg cgatgatcac gagcttgtcg ttctgctcct cctcggcgac
     6241 cgccggatcg agcagcccag aagcctgaac catcccactc accccttctt cttcagcagg
     6301 tagcggtaca ccccgccgtc ctcgccgtgc tcgaccagct cggtcccggt cgcccggcac
     6361 caggccggga tgtccgactg ggaaccgggg tcggtggcca ggacctcgaa cgtctcgccc
     6421 ggcccgagca gcttcagcgc ctgcgccgcc ttgaccaccg gcagcgggca cgacagcccg
     6481 cgggcgtcga ccgtggtgat cgtctgagac atcgctcagc cctccttctt cttctcgggt
     6541 accgccttcg gcctggtccc gatgtccggg acccggccct tcggcacggt cgcccagtag
     6601 atccggttga acagccactt cgccacgtgc cagtgcagcg acggcttcgg cgggatcggg
     6661 gggtgctcgt agtcgaaccg gagcaccgtg gtcctccggt tgccggtctc caggaagcac
     6721 atcacgcggc cctcgtagcc ctccttcggc gccgtccccc ggatcagcga cgccatccgg
     6781 ctggcgatca ccggcgcctc gaagtgtgcg gtcgacccgc tcttcgagat cggcaggttg
     6841 gtggcgtcgc ccatggcgaa gatccgctcc tgcccttcga cctgcagcgt ggtccggtcg
     6901 gtcgggatcc agcctcccgg gtcgccgagg ccggaggcct cgaccaccga ttgcccgcgg
     6961 tgcggcggaa cgatgacggc caggtcgtac tcggccttct cgccctcgag cgaggtgacc
     7021 acgccggccg ccggctccac ggtctcgacg ttgaagaacg tcgagagccc gatgccgcgc
     7081 gactccatga tcggctggac gaccttcgag gccgactcga tcgtgaacgc ccggttcagc
     7141 ggcgagagca gcgtcacctc gctgcggccc cggatgccac gatcgcgcag gtactcctcg
     7201 agcatgaaca cgaactcgac cggcgccggc gggcacttgt aggggatccc ggccacgccg
     7261 acgacgacct tgccgccgtc gaagcggtcg agctcctcgc gcagccggag cgccccctcc
     7321 agcgagtaga agtgctgggc gccctccgcc atcccgggga cctgctccgg caccaggcgg
     7381 gctccggtgg ccatcaccag gtagtcgtaa ggcagggatc cgccacgctg gagctccact
     7441 tcgccgcggt cggggtgcac ccgagcggcg ccctcgatcg cgagctccac gcccgggtag
     7501 agcagcgtgc gctcgtcgcg ggacagccac cggccgttcg cctggcccag cgcgacgtac
     7561 aggaaccccg gctggtagac gtgcatgccg gtggggtcga ccaccgtgac ggtggcgttc
     7621 cgcccgagct ccttgtgcag caggttcgcc gccagcgtgc cgccgacgcc accacccagg
     7681 acgacgatcc gcgcgggcat cgggtcaccg agccttgcgg atgacgaacc gggcgaagcc
     7741 gtcctccgga gcgacctcga ccaactcgtg gccggccttg gcgacccagg ccgggatgtc
     7801 ggtcttcgat ccctcgtcgc tcgacaggac ctcgatgacg tcgcccacgg cgccctcgcg
     7861 gatcgcgccg atcagggaca tcagcggtcc ggggcatgcc atcccgcgcg cgtcgatcac
     7921 ttgagtggta tcgatcgtcg tcatgattgt ctcctcctca gatgaatgcc atcgcgccag
     7981 ttgcatcgcc catgaacgct gcgacgcctt cgacgtcgtc cacgagcggg tcgagatcgt
     8041 ccttcgtgat tccgaacatc tccatcgaca acgcgcaggc gtggatggcg acgtcgcc
//
LOCUS       DASPWO010000037         6406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_685570, whole genome shotgun sequence.
ACCESSION   DASPWO010000037 DASPWO010000000
VERSION     DASPWO010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685570"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1946)
                     /locus_tag="VEO00_00890"
     CDS             complement(<1..1946)
                     /locus_tag="VEO00_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56589.1"
                     /translation="MTGPPARSRARPFLLLAATALLVAGFFLGVYFAFGYRVPIGFDV
                     ARYLDQTNAVARYGLVETAALRLPAPGLAFDSRVGFPVLVLSLSRVTGTSTFVWAAAL
                     PAAAAAATALAAGALLTSAARGRPWEAAAAAALVGVSPGVARMMAGTYTDNLLAAALV
                     VAAIAMALVAVRGAGGIVAPTALLAAAGIAHPAFFAVGLTVLAVTALVALPASWRAWR
                     RGTRPWRTPAARLALIVGLAGALAAVVVFVLLRTSVDRPVIQTPRLFRARLRQDLPLY
                     LLPVTVPAALAGAWWLARRERRANDRAPRAPLTVACAAWAALTLLAAATLLSRVRSVP
                     GQQVITVIRALPAHRFLAFLVPLPLLAGAALVGAGRALSGRARGGRALAAVAVAAGLA
                     GAVALGGVILYRTLADRGLVWMDPDKVAGAARAGAYLDAAGVPAGRPVIFVVDDRGPD
                     PGSLVSLMAHTIRAVQGTDRLPAVNVFVGRPDDLQAGRPTRIEEDDRGYNKVSNRQFR
                     SVRRVLDEDPVILMLEAFHPDYPAAAAADPGAVVAPGVLSLAGGGRASAAPPDVPSVP
                     TGALPLGALGAGSLVLLGLAGAGWAFAGGGLRAFEAAALAPAFGLAAVLFTGIVLDVA
                     GLRLGRAGGAIAVPVAALLGLAVW"
     gene            complement(1977..3596)
                     /gene="groL"
                     /locus_tag="VEO00_00895"
     CDS             complement(1977..3596)
                     /gene="groL"
                     /locus_tag="VEO00_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007384131.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HYU56590.1"
                     /translation="MPAKLLKFDEDARRALEAGIDKLADAVSITLGPKGRNVVLDKKW
                     GAPTITNDGVTIAKEVELEDPWENMGAQLAKEVATKTNDVAGDGTTTATVLAQAMVKR
                     GMKNVAAGANPMGLKRGIEKAVAAVVEALGAAAKDVESKDEIAQVAAISAADSSIGEV
                     IAEALDKVGKDGVVTVEESQTFGMELEFVEGMQFDKGFISPYFVTDPERQEAVFEDPY
                     ILLVNKKLSAVKDLLPVLEKVIQSGKPLVAVAEDVEGEALATLVVNKIRGTFPSVAVK
                     APGFGERRKAMLEDMAILTGSQVISEEVGLKLENATLDMLGRARKVVVTKDDTTIVEG
                     QGDAGEIQGRINQIKGEIENTDSDWDREKLQERLAKLSGGVALIKVGAATEVELKEKK
                     HRIEDALSATRAAVEEGIVAGGGVALIRTEGTLDALTLEGDEATGAAIVRQALAEPAR
                     LIAENAGFAGAVIVERIRAEGDGRGFNALTGTWGDLMKEGVVDPAKVTRSALQNAASI
                     AGLVITTESAVVEKPEEEEPTPGGHGGHDHY"
     gene            complement(3657..3959)
                     /gene="groES"
                     /locus_tag="VEO00_00900"
     CDS             complement(3657..3959)
                     /gene="groES"
                     /locus_tag="VEO00_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HYU56591.1"
                     /translation="MAKGFSLEPLEDRIVVKPGEEEETTVSGIVIPDTAKEKPQEGEV
                     MAVGPGRFEDGARVPMDVKVGDTVVYSKYGGTEVKVEGEEYLVLSARDVLAIVRKK"
     gene            4148..4843
                     /locus_tag="VEO00_00905"
     CDS             4148..4843
                     /locus_tag="VEO00_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56592.1"
                     /translation="MATETVQRRGGFAAAGRQVARERFGGVDAPATLTGMLVALSVEL
                     LLGGLVAAAVGAFGFQLGFTQGATELSLGGAIAGFAVMFVAFLFGGWAAGRMARYDGA
                     RNGMMVAVWALLLAAILVALGLWLGDRYDVLARARLPQWATGGRVTAGAAIGGLVAVG
                     VMLIGGALGGIWGASYHRRADRMVAEGVIAETIASETVPAATETDVVATETEPVPTET
                     VEPTGKKTIRRPI"
     gene            complement(4863..5312)
                     /locus_tag="VEO00_00910"
     CDS             complement(4863..5312)
                     /locus_tag="VEO00_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HYU56593.1"
                     /translation="MRSQTTEIRVLPERRLDIVDLTDDLRRAIKDGGITDGVAIAFCR
                     HTTAVLVVNEWEDGALQDLRARVEALVPADVYYAHDDLERRTQNLDEAHERANGRAHV
                     AAMLLGGTSQVIPIAGGEPVLGTWQRLLLIELDQPKDRVVSLQILGE"
     gene            complement(5335..6360)
                     /locus_tag="VEO00_00915"
     CDS             complement(5335..6360)
                     /locus_tag="VEO00_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU56594.1"
                     /translation="MTRPIRPLDSLSSIRTKLGVVILAAVGVVVLVQALGYRAGLPFL
                     LVGAASGALALAMVQILARGMTSPLREMAQATKAMARGDYSRRVTATSRDEVGELARA
                     FNSMAAELAEVDRMRRDLVANVSHELRTPISALQAVLENLVDGVEEPDPGTLGVMLGQ
                     VERLGRLVSQLLDLSRLESGVVPLDHKPFEVRPLLERAVREQELTARQAPAGVHLRLD
                     VEPAGLRLDGDPERVHQVMTNLLENAVRHSPPGDEVTVRARSNGAGVLIEVTDHGPGI
                     PEADASRVFERFYRADPARSSTDGGTGLGLAIARWIVDLHGGDIRAERNEPQGCRMVV
                     TLPRPAA"
BASE COUNT          857 a         2364 c         2329 g          856 t
ORIGIN      
        1 aaccacaccg cgagccccag gagggcggcg accgggacgg cgatcgcgcc ccccgcccgg
       61 cccaggcgca gcccggccac gtcgagcacg atgccggtga acaggaccgc ggcgagcccg
      121 aacgccggcg ccagggccgc ggcctcgaac gcgcggaggc cgccgccggc gaacgcccac
      181 ccggcgcccg ccaggccgag gagcacgagg gaaccggccc ccagggcccc cagcggaagg
      241 gcgccggtcg ggaccgacgg cacgtccggc ggcgccgccg acgctcgccc gccgccggcg
      301 agcgagagga cgcccggcgc gaccaccgcc ccggggtctg ccgcggccgc ggcggggtag
      361 tccgggtgga aggcctcgag catcaggatc accggatcct cgtccaggac gcggcggacg
      421 ctgcggaact gccggttgga gaccttgttg tagccgcgat cgtcttcctc gatccgcgtc
      481 ggacgcccgg cctgcagatc gtcgggccga ccgacgaaca cgttgaccgc cgggaggcgg
      541 tcggtgccct gcaccgcgcg gatcgtgtgc gccatcaggg agaccagcga gccgggatcg
      601 gggccgcggt cgtcgacgac gaagatcacc ggccgccctg ccgggacgcc ggccgcgtcc
      661 agataggccc ccgctcgcgc cgctccggcc accttgtccg gatccatcca caccaggccg
      721 cggtcggcga gcgtccggta gaggatcacc cctccgagcg ccaccgcgcc ggccaggccc
      781 gcagcgacgg cgacggcggc cagggccctg ccgcccctcg cccggccgga cagcgcccgg
      841 ccggctccca cgagcgcggc gccggccagg agcggcaagg ggacgaggaa ggccaggaag
      901 cggtgcgccg ggagggcccg gatcacggtg atcacctgct gcccgggaac ggaccggacg
      961 cgggagagga gggtcgccgc cgcgaggagc gtcagcgcgg cccaggccgc gcaggcgacg
     1021 gtcagcggcg cgcggggggc tcggtcgttc gcccgccgct cgcggcgggc gagccaccag
     1081 gcgcccgcga gcgccgccgg cacggtgacc gggaggaggt acagggggag gtcctggcgc
     1141 agccgcgccc ggaacagccg cggggtctgg atcaccgggc ggtcgaccga cgtgcgcagg
     1201 aggacgaaca ccacgaccgc cgccagggcc ccggcgagcc ccacgatcag ggcgaggcgg
     1261 gcggcggggg ttcgccaggg acgggtccct cgtcgccagg cccgccagct tgccggcagc
     1321 gcgacgagcg cggtcacggc gaggactgtc aggccgacgg cgaagaatgc cgggtgggcg
     1381 atccccgccg ccgcgagcag tgccgtgggc gcgacgatcc cgccggcgcc gcgcacggcc
     1441 accagggcca tggcgatcgc ggcgaccacg agggccgccg ccagcaggtt gtccgtgtag
     1501 gtccccgcca tcatccgggc gacgcccggc gagaccccga cgagcgcggc ggcggcggcc
     1561 gcctcccacg gccggccgcg cgccgcggag gtcagcagcg cgccggcggc cagcgcggtc
     1621 gcggcggctg cggcggccgg cagcgcggcg gcccagacga acgtcgacgt gccggtgacg
     1681 cgcgagaggg agagcaccag gaccgggaac ccgacccggc tgtcgaaggc cagcccgggg
     1741 gcgggcaggc ggagcgcggc ggtctcgacc agcccgtagc gggccaccgc gttcgtctgg
     1801 tccaggtagc gagcgacgtc gaacccgatc gggacgcggt agccgaaggc gaagtagacg
     1861 ccgaggaaga accccgcgac gagcagcgca gtcgcggcga gcaggaggaa gggacgggcc
     1921 cgggaacgag cggggggacc cgtcaacggg tcccccctgg aggtttccgg cacgtcctag
     1981 tagtggtcgt gccccccgtg gccgcccggc gtcggctcct cctcctcggg cttctccacc
     2041 acggcgctct ccgtggtgat caccagcccg gcgatggagg cggcgttctg cagcgcggag
     2101 cgggtcacct tggccgggtc gaccacgccc tccttcatca ggtcgcccca ggttccggtc
     2161 agcgcgttga agccacgccc gtcgccctcg gcccggatcc gctcgacgat caccgcgccg
     2221 gcgaagcccg cattctcggc gatcagccgc gcgggctcgg ccagcgcctg gcggacgatg
     2281 gccgccccgg tggcctcgtc gccctcgagt gtcagcgcgt cgagcgtgcc ctcggtccgg
     2341 atcagcgcca cgccaccgcc ggcgacgatc ccctcctcca ccgcggcgcg ggtggccgag
     2401 agcgcgtcct cgatgcggtg cttcttctcc ttcagctcca cctcggtggc cgcgcccacc
     2461 ttgatcagcg ccacgccgcc ggagagcttc gccagccgct cctgaagctt ctcgcgatcc
     2521 cagtccgagt cggtgttctc gatctcgccc ttgatctggt tgatccggcc ctggatctct
     2581 ccggcgtcgc cctggccctc gacgatcgtg gtgtcgtcct tggtgacgac caccttgcgc
     2641 gcccggccga gcatgtcgag cgtggcgttc tccagcttca gtccgacctc ctcggagatc
     2701 acctggctgc cggtcaggat cgccatgtct tcgagcatcg ccttgcgccg ctcgccgaag
     2761 ccgggagcct tcaccgcgac cgacgggaac gtgccgcgta tcttgttgac gacgagcgtg
     2821 gcgagcgcct cgccctcgac gtcctcggcg acggccacga gcggcttccc cgactggatc
     2881 accttctcga gcaccggcag caggtccttc accgcgctga gcttcttgtt caccagcagg
     2941 atgtaggggt cctcgaacac cgcctcctgc cgctcggggt cggtgacgaa gtacggggag
     3001 atgaagccct tgtcgaactg catgccctcg acgaactcga gctccatccc gaacgtctgg
     3061 gactcctcga ccgtgaccac gccgtccttg cccaccttgt ccagcgcctc ggcgatgacc
     3121 tcgccgatgg aggagtcggc ggcggagatc gccgcgacct gggcgatctc gtccttcgac
     3181 tcgacgtcct tggcggccgc gccgagggcc tcgaccacgg cggcgaccgc cttctcgatg
     3241 ccccgcttca gccccatcgg gttcgcgccg gcggccacgt tcttcatccc gcgcttgacc
     3301 atcgcctggg cgagcaccgt ggcagtggtc gtcccgtcgc cggcgacgtc gttcgtcttg
     3361 gtggcgacct ccttggccag ctgggccccc atgttctccc acgggtcctc cagctcgacc
     3421 tccttggcga tggtcacgcc gtcgttcgtg atagtgggcg ctccccactt cttgtccagc
     3481 acgacgttgc gccccttcgg cccgagcgtg atcgacaccg cgtcggccag cttgtcgatg
     3541 ccggcctcga gcgcccggcg ggcgtcctcg tcgaacttca gcagcttcgc aggcatcgaa
     3601 tgcctcctgc cggccccgtg gggccctcga tgggatggtg agcggccggt tgccgcttac
     3661 ttcttgcgga cgatcgccag cacgtcgcgg gccgacagga ccaggtactc ctcgccctcg
     3721 accttgacct cggtgccgcc gtacttggag tagacgacgg tgtcgcccac cttgacgtcc
     3781 atcggcaccc gcgccccgtc ctcgaagcgt ccggggccga cggccatcac ctcgccctcc
     3841 tggggcttct ccttggcggt gtcggggatg acgatgccgg agaccgtggt ctcctcctcc
     3901 tcgcccggct tgaccacgat gcggtcctcg agcggctcca ggctgaaccc cttggccatg
     3961 tctctcctcc tcggacgcga acggttggca ctctcgcgtt gagagtgcta acagtctagc
     4021 gacccgcccg ccgaacggtc aacgcccgcc ccgaccacgt ccccggacag cgcacccccc
     4081 gaggagggga agcccgcgtt tccgctcgct ccggggggga agttccttcg tggaggaagg
     4141 gggagccatg gcgacggaga cggttcagcg gcgaggcggg ttcgcggccg cggggcggca
     4201 agtggcgcgg gagcgcttcg ggggggtcga cgccccggcc accctgaccg ggatgctggt
     4261 ggcgctctcg gtcgagctgc tcctgggagg cctggtagcg gccgcggtcg gggccttcgg
     4321 gttccaactg ggcttcacgc agggtgccac cgagctgtcg ctcggcgggg ccatcgccgg
     4381 tttcgcggtg atgttcgtgg cgttcctgtt cggcggctgg gccgccgggc gcatggcgcg
     4441 ctacgacggc gcacgcaacg ggatgatggt ggcggtgtgg gcgctgctcc tcgccgccat
     4501 cctggtcgcg ctcgggctgt ggctcggcga ccgctacgac gtgctggcgc gggcccggct
     4561 gccgcagtgg gccaccgggg gacgggtgac ggccggcgcc gcgatcgggg ggctggtcgc
     4621 ggtgggcgtc atgctgatcg gcggagcgct cggcgggatc tggggcgcct cgtaccaccg
     4681 ccgggccgac aggatggtcg ccgagggagt gatcgccgag acgatcgcct cggagacggt
     4741 gccggcggcc accgagaccg acgtggtcgc caccgagacg gagccggtgc ccacggagac
     4801 ggtggaaccg accgggaaga agaccatccg ccgtccgatc tgaggcacgg ccggagcgcc
     4861 ggctactcgc cgaggatctg cagggagacg acccggtcct tcggctggtc gagctcgatc
     4921 agcagcaggc gctgccacgt gccgaggacc ggctcgccgc ccgcgatcgg gatcacctgg
     4981 gaggtccctc cgaggagcat cgccgccacg tgggcgcggc cgttggcccg ctcgtgcgcc
     5041 tcgtcgaggt tctgcgttcg gcgctcgagg tcgtcgtggg cgtagtagac gtccgcgggg
     5101 acgagcgcct ccacgcgggc ccggagatcc tgcagcgcgc cgtcctccca ctcgttcacc
     5161 acgagcaccg cggtcgtgtg ccggcagaag gcgatcgcca cgccgtcggt gatcccgccg
     5221 tccttgatcg cccgccgcag gtcgtcggtg aggtcgacga tgtcgagccg tcgctcgggc
     5281 agcacccgga tctcggtcgt ctgtgacctc atgagcccct cagattgaac cccatcatgc
     5341 tgccggccgg ggaagggtga cgaccatccg gcagccctgc ggctcgttgc gctcggcgcg
     5401 gatgtcgccg ccgtgcaggt cgacgatcca gcgcgcgatc gccaggccga ggccggtgcc
     5461 gccgtcggtc gaggagcgcg ccgggtcggc ccggtagaac cgttcgaaga cccgcgacgc
     5521 gtcggcctcg gggatcccag gcccgtggtc tgtgacctcg atcagcacgc cggcgccgtt
     5581 cgaccgggcc cgaaccgtga cctcgtcgcc cggcggcgag tggcgaaccg cgttctccag
     5641 caggttcgtc atcacctggt gcacccgctc ggggtcgccg tccaggcgca ggccggccgg
     5701 ctcgacgtcc aggcgcaggt ggacgcccgc aggggcctgc cgcgcggtga gctcctgctc
     5761 gcgcaccgcg cgctcgagga gcggccggac ctcgaacggc ttgtggtcga gcgggacgac
     5821 gcccgactcc agccgggaca ggtcgagcaa ctgcgagacc agccggccga ggcgctcgac
     5881 ctggccgagc atcacaccca gggtccccgg atcgggttct tcgaccccgt ccaccaggtt
     5941 ctcgagcacg gcctgcagcg cgctgatcgg ggtgcgcagc tcgtgggaca cgttcgccac
     6001 cagatcgcgg cgcatgcggt ccacctcggc cagctcggcg gccatcgagt tgaaggcccg
     6061 ggcgagctcc ccgacctcgt cgcgcgacgt ggccgtgacg cggcgcgagt agtcgccgcg
     6121 ggccatcgcc ttcgtggcct gcgccatctc acgcagcggc gaggtcatgc ctcgcgccag
     6181 gatctgcacc atcgccagcg ccagcgcgcc ggaggcggca ccgacgagca ggaacgggag
     6241 gccggcccgg tacccgagcg cctggacgag cacgacgacg cccacggcgg ccaggatcac
     6301 caccccgagc ttggtgcgga tcgaggagag ggagtcgagc gggcggatcg ggcgcgtcat
     6361 ctggggacct ccatggcata cccgaccccg tgaaccgtgc ggatca
//
LOCUS       DASPWO010000038         5526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_177093, whole genome shotgun sequence.
ACCESSION   DASPWO010000038 DASPWO010000000
VERSION     DASPWO010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177093"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VEO00_00920"
     CDS             <1..519
                     /locus_tag="VEO00_00920"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HYU56595.1"
                     /translation="GRAGTMTHDYKRNGTLDLFAALNVGSGEVLHDTRRSHTGRDVLA
                     FFRWIDMHVDPDLELHVILANLSAHKSQPVRDWLAHKKRRRWHLHFTPTSSSWLNLVE
                     CWFSVLSRKALTNTSFTSVAELETRIDCWVSHWNDNPEPFVWTKPADEILDTVARGQA
                     TLDRITKSATHH"
     gene            639..935
                     /locus_tag="VEO00_00925"
     CDS             639..935
                     /locus_tag="VEO00_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HYU56596.1"
                     /translation="MEANNTSGFNCGEATGEPVWSEHAYGRAVDVNPVQNPFIDGGEV
                     LPEAGHVYLDRTDVRPGMIVAGDPVVRAFAAIGWTWGGDYNSVKDYQHFSATGR"
     gene            1034..2431
                     /locus_tag="VEO00_00930"
     CDS             1034..2431
                     /locus_tag="VEO00_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HYU56597.1"
                     /translation="MEARSWAEPLVPRDAAFLYLEGQASPYHVAALMVFGGPPPARDE
                     VLEWIGARTGLVPRLRQRLALAPLTGRPFWVDDPHFDLEYHVRFEELPAPGDDRALAA
                     LTGELVARHLSRFRPLWEIWVLEPTAAGGFAVLMKSHHSAVDGVAGIRDLTALFAPLP
                     PDAVPLLPPRPVPSELGLSVRSALHAAETPIALLRAARAAAGAPARTLRRVAGTAVGV
                     ARVGLTALHPAPHVPLNVLIGPNRSVEFVRASLQDFKDVKAALGGTVNDVVLAVTAGG
                     LRRWMEARDLPIGPDLVALVPISLRGGGSGRGSTGAGNEVTGMLAPLPVGLENPLTRL
                     ARVRRATALAKVSGQSAGASALLNSLPLLPAPVTAALAGRLIRGQRSFNVSMPNLRGP
                     EEPLQLAGRQAVDVFALPPLSANAAFILAAMSHAGAMTFGLLADRDAVPDLGVIAEGI
                     EKSIVELVQAAREVG"
     gene            2433..3464
                     /locus_tag="VEO00_00935"
     CDS             2433..3464
                     /locus_tag="VEO00_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HYU56598.1"
                     /translation="MRLTTHPVRSRDDRRAFVRLPHRLYARDPLWVAPIWSDLRARLD
                     LDRAPFFAHGRAELFLARRGGEPVGRISAHVDDAYTRLHSKPAHPDRAGFFGFFECDD
                     DGAAAEALIDAAAEWLADRGCDRMIGPASFTLNDEAGLLIDGFDRPPMFLMAYNPPYY
                     EPLLEKSGLAGVQDLFAYRLDASSDPPPDVERFARTAEAEFTFRTIDLRRFDEEMRRF
                     LEVYNAAWEANWGFAPFSESEIREHAKRLRPIVDPNLVFVVECGSEPVAVGLTLPDLN
                     EALISVHGRLGPVAAARVLWRARRRRWSACRVVALGVKKEFRRTGIGAHLYIDTLSAA
                     AAAATAGAR"
     gene            complement(3562..4842)
                     /locus_tag="VEO00_00940"
     CDS             complement(3562..4842)
                     /locus_tag="VEO00_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56599.1"
                     /translation="MVRQVRRALPFVLAAALILGPAIPAHAAAGDPDPGFGAGGEAFA
                     GFPGRDEAGHAVAVAANGKVIVAGTSESGAFDVEVMRFTTGGVLDNTFDGDGIRKTSF
                     GGTDHGRAVAIQPDGKVVVAGYSLNPAPNSSAIVARYTTTGAFDASFSGDGKKVVNWP
                     GVTQSGAYGVAIQADGKIVLAGFALPGDFAVARLNPNGTYDNTFDGDGRVTIDIGPND
                     TAFAVAIQDDQTIVVAGNAIADDASAVRFGVARLNTDGTLDGSFSGDGVLRTPFSGED
                     AYALGVAVQGDGKIVAAGLVTSATATDFALVRYNANGALDTSFAVGGKRSTSFDQNDS
                     ANGVAIQGDGKIVAGGATYDGSDTDFALARYTTGGNLDAGFGVGGKVTTGFGAPDFAD
                     GVAISPVDGKIVLAGGTLAGPDPSNFAVVRYLVA"
     gene            complement(4957..>5526)
                     /locus_tag="VEO00_00945"
     CDS             complement(4957..>5526)
                     /locus_tag="VEO00_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HYU56600.1"
                     /translation="FAYSDNGAISRFGVARLTTVGQPDLTFSGDGELRTGFTGGAATA
                     QAVVVQPNGRIVVAGQVESTTTQDFALVRYTVAGALDSSFGNGGRRTTSFGQSDAATG
                     VAIQADGRIVAAGYASGSTADFALARYGPGGNLDGSFGNGGRVVTDISGEDTGSDVAI
                     SPLDGKILVAGTTYGGATVDIAIVRYLAA"
BASE COUNT          742 a         2035 c         1939 g          810 t
ORIGIN      
        1 gggcgggccg ggacgatgac ccacgactac aagagaaacg gcaccctgga cctgttcgcc
       61 gcgctgaacg tgggctccgg ggaggtgctg cacgacaccc gccgctccca caccggccgt
      121 gacgtgctgg cgttcttccg ctggatcgac atgcacgtcg accccgacct ggagctgcac
      181 gtcatcttgg ccaacctgtc ggcccacaag agccagcctg tccgtgactg gctggcgcac
      241 aagaaacgcc gccgctggca cctgcacttc accccgacca gctcgtcatg gctcaacctc
      301 gtcgagtgct ggttctccgt gctcagccgc aaagccttga ccaacaccag cttcacctcc
      361 gtcgccgaac tcgaaacccg gatcgactgc tgggtctccc actggaacga caaccccgag
      421 cccttcgtct ggaccaaacc cgccgacgag atcctcgaca cagtcgcccg cggacaagcc
      481 accctcgacc ggatcaccaa atccgcgaca caccactagt cctccacgcc gacgcggctg
      541 cggacatcct ccgcgtcttc cgcactctgt tcgaggcgcg cttaccgatc gagcggatcg
      601 agctggtcga cgcgtacggc ggcgacgacg tgctgtcgat ggaggccaac aacacatccg
      661 ggttcaactg cggggaggcc acgggggagc ccgtgtggtc cgagcacgcg tacggacgtg
      721 cggtcgacgt gaacccggtt cagaacccgt tcatcgacgg cggcgaggtc cttccggagg
      781 ccggtcacgt gtacctggac cgcaccgacg ttcggcccgg gatgatcgtc gctggcgatc
      841 cggtcgtccg cgccttcgcc gccatcgggt ggacctgggg cggcgactac aactcggtca
      901 aggactacca gcacttctcc gccaccggcc ggtaggcgcc ggcacgccgg aggtcccggc
      961 ggacgggggc ccaacggcag gagccgggga gcggcgcgcc gcgacaggat cggggcggcg
     1021 gaggtggtgg aacgtggagg cccggagctg ggcagagccg ctggtgccgc gcgacgcggc
     1081 gttcctgtat ctggaggggc aggcgtcgcc gtaccatgtc gccgccctga tggtcttcgg
     1141 cgggccgccg ccggcgcgcg acgaggttct cgagtggatc ggcgcgcgca ccggtctggt
     1201 gccgcgcctg cgccagcgcc tcgccctcgc gccgctcacc ggccggccgt tctgggtgga
     1261 tgacccgcac ttcgacctcg agtaccacgt ccggttcgag gagctcccgg cgccggggga
     1321 cgatcgcgcc ctcgccgcgc tgaccggaga gctcgtcgcc cgtcacctca gccggttccg
     1381 gccgctgtgg gagatctggg tcctcgagcc aacggcggcc ggcggcttcg cggtgctgat
     1441 gaagtcacat cacagcgcgg tggacggcgt ggccggcatc cgcgacctga ccgctctctt
     1501 cgcgccgctg cccccggacg ccgtcccgct cctcccgccg cgtccggtcc cgtccgagct
     1561 cggcctgtcc gtgcgctcgg ccctgcacgc cgcggaaacg ccgatcgccc ttctccgcgc
     1621 ggcccgggcg gccgccggtg ccccagcccg cacgctccgg cgggtcgccg gcaccgcggt
     1681 gggcgtcgcg cgggtcgggc tcacggcgct ccaccccgcc ccgcacgtgc cgctgaacgt
     1741 actgatcggc ccgaaccgct ccgtcgagtt cgtgcgcgcc tcgttgcagg acttcaagga
     1801 cgtcaaggcc gcgctcggcg ggaccgtcaa cgacgtggtc ctggcggtga cggccggtgg
     1861 cctgcgccgc tggatggagg cccgcgacct cccgatcgga ccggacctgg tggccctggt
     1921 gccgatctcg ctccggggag gcggctccgg acgtggctcc accggcgccg ggaacgaggt
     1981 caccgggatg ctggccccgc tcccggtcgg tctggagaat cccctgaccc gcctggcgcg
     2041 ggtgcggcgg gcgaccgccc tggcaaaggt ctccggccag tcggccgggg cgagcgcgtt
     2101 gctgaactcc cttccgctgc tcccggcgcc ggtcaccgcg gcgctcgccg gccgactgat
     2161 ccggggccag cgctcgttca acgtgtcgat gccgaacctg cgcggcccgg aggagccgct
     2221 gcagctcgcg gggcggcagg cggtggacgt cttcgcgctg ccaccgctct cggcgaacgc
     2281 ggcgttcatc ctggcggcca tgtcgcacgc cggcgcgatg acgttcggcc tgctcgccga
     2341 ccgcgacgcg gtgcccgacc tcggcgtgat cgccgagggc atcgagaagt cgatcgtgga
     2401 actggtccag gcggccagag aggtgggctg acgtgcggtt gacaacccat cccgtgcggt
     2461 cccgcgacga ccggcgagcc ttcgtccggc tgccgcaccg gctgtatgcg cgagacccgc
     2521 tctgggtcgc gccgatctgg tcggacctcc gtgcacgcct ggacctggac cgcgccccct
     2581 tcttcgcgca cgggcgtgcg gagctgttcc tggcccgtcg cggcggggaa ccggtcggcc
     2641 gcatatcggc gcacgtcgac gacgcctaca cgcgcctgca ctcgaagccc gctcacccgg
     2701 accgcgccgg cttcttcggc ttcttcgagt gcgacgacga cggcgcggcg gccgaggctc
     2761 tcatcgatgc cgcggcggag tggctcgccg accgcgggtg tgaccggatg atcgggccgg
     2821 cgtcgttcac gctgaacgac gaggccgggc tgctgatcga cgggttcgac cggccgccga
     2881 tgttcctgat ggcgtacaac ccgccgtact acgagccgct gctggagaag agcgggctcg
     2941 ccggcgtgca ggacctgttc gcctaccggc tggacgcctc cagcgatccg ccacccgatg
     3001 tcgagcggtt cgcgcgcacg gcggaggccg agttcacgtt ccggacgatc gacctgcggc
     3061 ggttcgacga ggagatgcgg cggttcctgg aggtctacaa cgccgcctgg gaggcgaact
     3121 ggggcttcgc gccgttctcc gagagcgaga tccgcgagca cgccaagcgg ctccgcccga
     3181 tcgtcgaccc gaacctggtg ttcgtcgtcg agtgtgggtc ggagccggtg gccgtcgggc
     3241 tcacccttcc cgacctgaac gaggccctga tcagcgttca cggtcgcctc ggcccggtcg
     3301 ccgccgcgcg cgtgctgtgg cgggctcgcc ggcgccgctg gagcgcctgc cgcgtggtcg
     3361 ccctcggcgt caagaaggag ttccggcgca ccgggatcgg cgcccatctc tacatcgaca
     3421 cgctcagcgc cgccgccgcg gcggctaccg ctggggcgag atgagctgga tcctggagtc
     3481 gaaccacgcc atgaaccggg cgatccggca catgggcggg gaggtctaca agacgtatcg
     3541 catgtacgag cgcccgatcg gtcaggccac caggtagcgg accaccgcga agttcgacgg
     3601 atccggaccg gccagcgtcc ccccggccag cacgatcttg ccgtcgaccg gcgagatcgc
     3661 caccccgtcc gcgaagtcgg gcgccccgaa cccggtggtc accttccctc ccacgccgaa
     3721 cccggcgtcc aggttcccac cggtcgtgta ccgggcgagc gcgaagtccg tgtcgctgcc
     3781 gtcgtaggtc gctccgccgg ccacgatctt cccgtcgccc tggatcgcca ccccgttcgc
     3841 gctgtcgttc tggtcgaagc tcgtcgaccg cttgccgccg acggcgaacg aggtgtcgag
     3901 cgccccgttc gcgttgtagc ggactagggc gaagtcggtg gccgtcgccg acgtcacgag
     3961 gccggcggcg acgatcttgc cgtcgccctg cacggcgacg cccagcgcgt aggcgtcctc
     4021 gccggagaac ggggtccgca ggacgccgtc cccgctgaac gacccgtcga gcgtgccgtc
     4081 ggtgttcagg cgggccaccc cgaaccggac ggccgaggcg tcgtcggcga tggcgttccc
     4141 ggccacgacg atcgtctggt cgtcctggat ggcgacggcg aacgccgtgt cgttcggccc
     4201 gatgtcgatc gtgacccggc cgtcgccgtc gaaggtgttg tcgtacgtgc cgttcgggtt
     4261 gagacgggcg acggcgaaat cgcccggcaa ggcgaacccg gccaggacga tcttcccgtc
     4321 ggcctggatc gccaccccgt aggcgccgga ctgcgtgacg ccgggccagt tgacgacctt
     4381 cttgccgtcg ccgctgaacg acgcgtcgaa cgcgccggtc gtcgtgtagc gggccacgat
     4441 cgcggagctg ttcggcgccg ggttcagcga gtagcccgcc accacgacct tgccgtcggg
     4501 ctggatcgcc accgcccgcc cgtggtccgt gcccccgaag ctagtcttgc ggatcccgtc
     4561 gccgtcgaac gtgttgtcga ggacgccgcc cgtcgtgaag cgcatcacct cgacgtcgaa
     4621 cgccccgctt tcgctggtgc cggcgacgat gaccttcccg ttggccgcca cggccacggc
     4681 gtgcccggcc tcgtcgcgcc cagggaagcc ggcgaaggcc tccccgccgg ccccgaaccc
     4741 ggggtcgggg tcgcccgccg cggcatgggc cgggatggcc ggtccgagga tcagggcggc
     4801 cgccaggacg aagggaaggg cacggcgaac ctgccggacc atgacggctc ctttgctggg
     4861 gggtgccggc acgcacggta cgcccggctt cgcgccgcgg tcaatgctcg accggcggga
     4921 gactggcggg ggccgtcgag ctcgaacccc ggccgttcag gccgcgaggt agcggacgat
     4981 ggcgatgtcg accgtggcgc cgccgtaggt cgtgccggcc accaggatct tgccgtcgag
     5041 cggcgagatc gccacgtcgg agcccgtgtc ctccccggag atgtcggtga ccacccggcc
     5101 gccgttgccg aagctgccgt ccaggttccc gcccggcccg tagcgcgcga gggcgaagtc
     5161 ggcggtcgag cccgaggcat acccggcggc gacgatcctc ccgtcggcct ggatcgccac
     5221 cccggtcgcg gcgtcggact gcccgaagct cgtcgtccgg cgcccgccgt tgccgaacga
     5281 actgtccagg gcgcccgcga ccgtgtagcg caccagtgcg aagtcctggg tcgtggtcga
     5341 ctccacctgg ccggcgacca cgatcctccc attgggctgg acgaccacgg cctgggcggt
     5401 ggcggctccg ccggtgaagc cggttcgcag ctccccgtcc ccactgaacg tcaggtcggg
     5461 ctgcccgacg gtcgtcagcc gggcaacgcc gaaccgcgag attgcgccgt tgtcggaata
     5521 cgcgaa
//
LOCUS       DASPWO010000039         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_773865, whole genome shotgun sequence.
ACCESSION   DASPWO010000039 DASPWO010000000
VERSION     DASPWO010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773865"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VEO00_00950"
     CDS             <1..283
                     /locus_tag="VEO00_00950"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HYU56601.1"
                     /translation="MEQVALVHPRVVVTLGNFATRVILDRPVSISRVRGEAFTWNGRT
                     VIPTFHPAAVLHGGGEAGRQMELIRRDFATLKELLAQPAPIVEEQLGLF"
     gene            288..773
                     /gene="tsaE"
                     /locus_tag="VEO00_00955"
     CDS             288..773
                     /gene="tsaE"
                     /locus_tag="VEO00_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HYU56602.1"
                     /translation="MAPTLQVVTASPSETRAVGAALAPLLAPRDLVSLGGDLGAGKTT
                     FVQGAAAGLGVAEPVTSPAFVLVREYRGAIPVVHVDVYRLNRMQEVLDLGFEEYLDSG
                     AVMFVEWGDVIEALFPQTFLAIEFTTLAGDERRLSFHPGGPAWAGRWERLEQALDGWA
                     A"
     gene            761..1480
                     /gene="tsaB"
                     /locus_tag="VEO00_00960"
     CDS             761..1480
                     /gene="tsaB"
                     /locus_tag="VEO00_00960"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HYU56603.1"
                     /translation="MGGLMLVLGIETSTPQTSVALGTEQGIVASTVLTRGRPGHEVAV
                     PAVSHLLRWSEVPPGRIGGIAIGLGPGLFTGMRVGIATGKTLAQVLRVPIVGMASLDV
                     LAFDVRYSRRPIVAVIDARRKEVFYAFYRPVPGGVARETGFQVGSPEELAAEIGSRRE
                     EMLLVGNGALLYRRTLEEELGSQVELASMAHAYPEAAALVELAVPRFVREEAGRPEDL
                     QPYYVRKSDAEIAWDKRARTA"
     gene            1497..2012
                     /gene="rimI"
                     /locus_tag="VEO00_00965"
     CDS             1497..2012
                     /gene="rimI"
                     /locus_tag="VEO00_00965"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HYU56604.1"
                     /translation="MRRRHLKGVLAIEREVYPRPWSPNLFLSEMAEPRNRAYLVARLG
                     RDVVGYAGLMSYGEEAHVTTIAVDPRHQRRKIGTRLLYELISRAREMGAEAVSLEVRM
                     SNWGAQRMYGRFGFRPVGVRKGYYQETDEDALVMWADGVNTDEYATQLERIRRAAGFP
                     AEAAPRSEAES"
     gene            2009..>2560
                     /gene="tsaD"
                     /locus_tag="VEO00_00970"
     CDS             2009..>2560
                     /gene="tsaD"
                     /locus_tag="VEO00_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HYU56605.1"
                     /translation="MRVLGIETSCDETAVAVVDDGEKVLASLIAGQAALHGRFGGVVP
                     ELASRAHLETINPLLDAALAEAQTDLHELDGVAVTVGPGLVGALLVGMATAKAVALAL
                     DLPFIGVNHLEGHIYANVLEHGHPDHPYVAFIVSGGHTMLVHVPEEHRYELLGQTLDD
                     AAGEAFDKVARLLGLGFPGGPALD"
BASE COUNT          331 a          882 c          976 g          371 t
ORIGIN      
        1 gatggagcag gtcgcgctgg tccacccccg ggtcgtggtc acgctgggga acttcgccac
       61 gcgggtgatc ctcgaccggc cggtgtcgat ctcgcgggtg cgcggcgagg cgttcacctg
      121 gaacggccgc acggtgatcc ccacgttcca cccggccgcc gtcctgcacg gcggggggga
      181 ggccggccgc cagatggagc tgatccgccg ggacttcgcg accctgaagg agctcctggc
      241 ccagcccgca ccgatcgtcg aggaacagct ggggctgttc tgaggggatg gccccgaccc
      301 tgcaggtggt gacggcgtcg ccgtcggaga cccgggccgt cggcgcggcc ctggcgccgc
      361 ttctcgcgcc ccgcgacctg gtcagcctgg gcggcgacct cggcgccggg aagaccacgt
      421 tcgtgcaggg cgcggcggcc gggctcggcg tggccgagcc cgtcacgagc ccggcgttcg
      481 tgctggtccg tgagtaccgc ggcgcgatcc ccgtggtgca cgtcgacgtc tatcgcctga
      541 accggatgca ggaggtcctg gacctcgggt tcgaggagta cctggactcg ggggcggtga
      601 tgttcgtcga gtggggcgac gtgatcgagg cgctcttccc gcagaccttc ctggcgatcg
      661 agttcaccac gctggccggc gacgagcgcc gtctgtcgtt ccaccccggc gggccggcct
      721 gggcgggtcg ctgggaacga ctggagcagg ccctcgacgg gtgggcggcc tgatgctcgt
      781 cctcgggatc gagacgtcca cgccgcagac ctcggttgcg ctcgggaccg agcaggggat
      841 cgtcgcctcc acggtgctca cgcggggccg gcccgggcac gaggtggccg tcccggcggt
      901 ctcccacctc ctgcgctggt ccgaggtgcc tcccgggcgg atcggcggga tcgcgatcgg
      961 gctcgggccg ggcctgttca ccggcatgcg ggtggggatc gccacgggca agacgctggc
     1021 ccaggtcctg cgggtgccga tcgtcgggat ggcctcgctc gacgtgctgg cgttcgacgt
     1081 gcgctactcg cgccgcccga tcgtcgcggt gatcgatgcg cgccgcaagg aggtgttcta
     1141 cgccttctac cggccggtgc cgggcggggt ggcgcgggag acggggttcc aggtcgggtc
     1201 gccggaggag ctggccgccg agatcgggtc ccgccgtgag gagatgctcc tcgtcggaaa
     1261 cggcgcgctg ctctatcgtc ggacgctgga ggaggagctg ggcagccagg tggagctggc
     1321 ctcgatggcg cacgcgtacc cggaggctgc ggccctagtc gagctcgccg tcccgcgctt
     1381 cgtgcgggag gaggcgggcc ggccggagga tctgcagccc tactacgtga ggaagtcgga
     1441 tgcggagatc gcctgggaca agcgagctcg gaccgcctga actcgagatc acgcggatgc
     1501 gccggcgcca cctgaagggc gtgctggcca tcgagcgcga ggtctacccc cgcccgtgga
     1561 gcccgaacct gttcctctcc gagatggcgg agccgcgcaa ccgcgcctac ctcgtcgccc
     1621 ggctgggccg cgacgtcgtc ggctacgccg gcctgatgtc ctacggggag gaggcgcacg
     1681 tcaccacgat cgccgtcgac ccgcggcacc agcgccgcaa gatcggcacg cgcctgctct
     1741 acgagctgat ctcccgcgct cgcgagatgg gcgcggaggc cgtctcgctc gaggtgcgca
     1801 tgagcaactg gggcgcgcag cggatgtacg ggcgcttcgg gttccgtccg gtcggggtgc
     1861 gcaagggcta ctaccaggag acagacgagg acgcgctcgt gatgtgggcc gacggcgtga
     1921 acacggacga gtacgccacc cagctcgagc ggatccgacg ggcggcgggg ttccccgccg
     1981 aggccgcccc gcgctcggag gccgagtcgt gagggtgctc ggcatcgaga cctcctgcga
     2041 cgagaccgcc gtcgcggtgg tcgacgacgg cgagaaggtc ctggcgagcc tcatcgccgg
     2101 ccaggcggcc ctgcacgggc gcttcggcgg cgtggtgccg gagctcgcgt cccgcgcgca
     2161 cctcgagacg atcaacccgc tcctcgacgc ggcgctcgcc gaggcgcaga ccgatctgca
     2221 cgaactggac ggggtggccg tcacggtcgg gcccggcctg gtcggtgcgc tgctcgtcgg
     2281 gatggccacg gcgaaggcgg tggcgctggc cctcgacctc cccttcatcg gcgtgaacca
     2341 cctcgagggc cacatctacg cgaacgtgct ggaacacggg cacccggacc acccctacgt
     2401 cgcgttcatc gtctccggcg gccacacgat gctcgtgcac gtgcccgagg agcaccgcta
     2461 cgagctgctc gggcagacgc tcgacgacgc ggccggcgag gccttcgaca aggtggcccg
     2521 gctgctgggg ttgggcttcc cgggcggtcc ggctctcgac
//
LOCUS       DASPWO010000040         5994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1193620, whole genome shotgun sequence.
ACCESSION   DASPWO010000040 DASPWO010000000
VERSION     DASPWO010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193620"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VEO00_00975"
     CDS             complement(<1..321)
                     /locus_tag="VEO00_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56606.1"
                     /translation="MEGDSATRPLGRALLGLLSVASVLVLVAGAQLFVLTDHTDRWFA
                     WTIGAPLTAATLGGFYWASFVTVRLAMRERTWAAARASLLGVLAFTILTMAATLIHLG
                     TFHLS"
     gene            630..1184
                     /locus_tag="VEO00_00980"
     CDS             630..1184
                     /locus_tag="VEO00_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HYU56607.1"
                     /translation="MTRKIIAGEFMALDGVLESPDKWSMPYFNDEVGAIIGTNMERSD
                     AMLMGRRTYEEWAAYWPGKTAEDDEFAPYINSVAKFVVSTTLTSVEWEGTTLLNGDFR
                     EDITKLKHQPGKDIAVSGSITLVSSLLREGLLDELSLLVSPIVVGAGKRLFEDPSGPV
                     GLKLIESRTLSNGVVALTYGLAEA"
     gene            1615..2661
                     /locus_tag="VEO00_00985"
     CDS             1615..2661
                     /locus_tag="VEO00_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004926498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU56608.1"
                     /translation="MSSNETIRTEGLVKRYGKVTALDGIDLAVPQGTVLGLLGPNGAG
                     KTTAVRILTTLLEPDEGRAVVAGLDVVADADKVRERIGLSGQNAAVDEHLTGFENLDM
                     VGRLYHLGKQRSRERARELLERFDLEDAGDRPTKTYSGGMRRRLDLAAALVAKPEVLF
                     LDEPTTGLDPRSRVQMWGTIHELVHGGSTVLLTTQYMEEADRLADDIVVIDHGRVIAH
                     GTANTLKAQVGGERVEVVLSEGADLPKARVLLGGLCHAEPQVDEGIRMLTAAVVSDGA
                     DVLKRVLEKFAEANVQIADVGLRRPTLDDVFLSLTGHVAEEEPPGEDGSADGDGSGGG
                     RGRRSEDRVKEAVQ"
     gene            2658..3461
                     /locus_tag="VEO00_00990"
     CDS             2658..3461
                     /locus_tag="VEO00_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU56609.1"
                     /translation="MSAVVRVFADGAVVAKRNLIKIKRVPDILVWTTMSPIMFVLLFG
                     LVFGSSIHIPGVSYREYLIAGIFAQTVIFGSTFTGVGLADDMTKGVIDRFRSLPMARS
                     AVLIGRTASDLVYNSISILVMSVTGLIVGWRIRTGVLPAIRGFLLLLVFAYAISWIMA
                     WVGLKVRAVEVVQNASFMFIFPVTFVANTFVRSELLPGPLQTFAEWNPVSAVAQGVRE
                     AFGNTGTTPKPTVWSLAHPIAYSLIWVAIILAIFVPLSIRQFRKAASRQ"
     gene            3508..3762
                     /locus_tag="VEO00_00995"
     CDS             3508..3762
                     /locus_tag="VEO00_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYU56610.1"
                     /translation="MADVPARDLRNDVSAVLRRVEGRERLRVTVSGRPVAELVPLAGR
                     PGSIRWDAFLENAERWRADAGLAKDLAALLPETTDDVPSP"
     gene            3759..4166
                     /locus_tag="VEO00_01000"
     CDS             3759..4166
                     /locus_tag="VEO00_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYU56611.1"
                     /translation="MTGRRPPRAELGLADTSLFIAFEQKRHLAGSPPESIAISVITVG
                     ELRLGVLAADDGPTRARRLETLSRAEALEPLPVDALVAHAWATLRLALRDAGRRMPLN
                     DSWIAATAIAHGIPVVSQDGDYDGVPGVEVIRV"
     gene            4214..5299
                     /locus_tag="VEO00_01005"
     CDS             4214..5299
                     /locus_tag="VEO00_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HYU56612.1"
                     /translation="MLLRRRRLGALEAVERLVAVQAQVPRDPYVALWSRLEGFRPSVL
                     SAALEGRRAVRMTLLRATLHLVTARDAVALRPLFQPVVERMFFSQRAFRRAAEGVDLD
                     EVVRLFRELLEERPGSRVALARAVAERWPDRDASSLGYAMYFLPTVQVTPRGLWGKSG
                     RAAVTTLEHWLGSGPLGPGAAPSELVARYLAVFGPATPADFSSWSGLAGVREVFEEFR
                     PRLRTFRDEQGRELFDIPRAPLPDPETPAPVRLLPEYDNALLGHADRSRVIPEGIPQW
                     TDVGWGTVLVDGFMAARWKLDREQVGATLRVEPFRRLTRAERAQVTEEGVRLAEFLEP
                     TGRPGVQLAGQIEPSAAERRPNSPRNR"
     gene            5454..5890
                     /locus_tag="VEO00_01010"
                     /pseudo
     CDS             5454..5890
                     /locus_tag="VEO00_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
BASE COUNT          900 a         2042 c         2192 g          860 t
ORIGIN      
        1 cgagaggtgg aaggtcccca ggtggatcag ggtcgcggcc atggtgagga tcgtgaaggc
       61 cagcaccccc aacagcgagg cccgggccgc ggcccaggtc cgctcgcgca tggccagccg
      121 caccgtcacg aagctcgccc agtagaaccc gcccagcgtg gccgcggtga gcggcgcccc
      181 gatggtccac gcgaaccacc gatcggtgtg atcggtcagg acgaacagct gtgcccccgc
      241 gacgagcacc agcaccgacg ccaccgagag cagcccgaga agcgcgcgac cgagcggccg
      301 tgtcgccgag tccccctcca tgtccggagc atgatgggtc atcggctcgt gccgcaatcc
      361 caaccttccc cgaaggagga tcggtcagtg gaaccggatc gagcggcagg tccttcagcg
      421 ccgcgacccg ccgaggagag cgagcagatg cgctccgccc tcgccttcga cggcgccggc
      481 cccgccgcat ggcgtcgacc ctgccggcga actcacgcgc ctcgacgatc gggatccccc
      541 tccacgggga gaggtcgcgg ccgatgagtt cggcgccggc gcggggtctg catcacggac
      601 gaacaggaac gaaccggaag gaggaggtcg tgacgaggaa gatcatcgcg ggcgagttca
      661 tggccctcga cggggtgctc gagtcgcccg acaagtggag catgccctac ttcaacgacg
      721 aggtgggcgc gatcatcggg acgaacatgg agcgctccga cgcaatgctg atggggcggc
      781 gcacgtacga ggagtgggcg gcgtactggc ccggcaagac cgccgaggac gacgagttcg
      841 cgccgtacat caacagcgtg gcgaagttcg tggtctccac caccctgacg tcggtggagt
      901 gggagggcac cacgctgctg aacggcgact tccgggagga catcacgaag ctgaagcacc
      961 agccgggcaa ggacatcgcg gtctcgggaa gcatcacgct ggtgagctcg ctgctccgcg
     1021 agggcctgct cgacgagctc agcctgctgg tctcgccgat cgtggtcggc gccggcaagc
     1081 gcttgttcga ggacccatcg ggcccggtgg gcctgaagct gatcgaatcc cgaacgctca
     1141 gcaacggcgt ggtcgcgctg acctacggcc tggcggaggc gtaagccgcg cagtcacccc
     1201 gtgtcgtgtg acgccgtgcg atcggcgacc aactgctgga cgcgctggtg cgaaaggccc
     1261 atgagaacgg ccgcgtcgcg cagcgagagc cccaatgtcg tcaccaggtc acccgccacg
     1321 atggcggtcc gctccttcgc ctcctcctag gcccgcgcgg ctcggtctct cgactcccgg
     1381 gcgcgccgga gctcggcgac gtgagtatca tcgccgcccc gaccgatgag ttccgccccc
     1441 cggggcggtc tgaaggagca ggtccgacga cgatgagaag agatcaggcc atgagcaggc
     1501 cgacgggaat acgaaggtct cgctccgcct tgtggacagc cagacgacca cgaagggcgt
     1561 cgtgatcctg acctacgagc cggcggaggc ggacgctcag ggaggcgagg gcagttgagc
     1621 tcgaacgaga cgatccggac cgaggggctg gtcaagcgct acggcaaggt gaccgcgctc
     1681 gacggcatcg acctggccgt cccgcagggg accgtgttgg ggctcctcgg gccgaacggc
     1741 gcggggaaga cgacggcggt tcgcatcctc acgactctgc tggagcccga cgagggccgg
     1801 gcggtcgtcg ccgggctcga cgtcgtggcc gacgccgaca aggtgcggga gcgcatcggg
     1861 ctctccgggc agaacgcggc cgtcgacgag cacctgaccg ggttcgagaa cctcgacatg
     1921 gtgggccggc tgtaccacct ggggaagcag cggtcccgcg aacgcgcacg ggagctgctc
     1981 gaacggttcg acctggagga cgccggcgac cggccgacga agacctactc cggcggcatg
     2041 cggcggcgcc tcgacctggc cgcggcgctg gtggcgaagc ccgaggtcct gttcctcgac
     2101 gagccgacca cggggctcga tccccggagc cgcgtccaga tgtggggcac catccacgag
     2161 ctggtccacg gcgggtcgac cgtgctgctg acgacgcagt acatggagga ggcggaccgg
     2221 ctggccgacg acatcgtcgt catcgaccac gggcgggtga tcgcccacgg gacggcgaac
     2281 acgctgaagg cccaggtggg cggggaacgg gtcgaggtgg tcctctcgga gggcgccgac
     2341 ctcccgaagg cccgggtgct cctgggcggc ctctgccacg ccgagcccca ggtcgacgaa
     2401 gggatccgca tgctgaccgc ggcggtggtg agcgacggcg cggacgtgct gaagcgggtc
     2461 ctggagaagt tcgccgaggc gaacgtgcag atcgcggacg tcgggctccg ccggccgacg
     2521 ctggacgacg tcttcctgtc gctcacgggc cacgtcgcgg aggaggagcc tcccggcgag
     2581 gacggctccg ccgacggcga cgggtccggc ggcggcaggg gccgacgaag cgaggaccgg
     2641 gtgaaggagg cggtccagtg agtgccgtgg ttcgcgtctt cgccgacggc gccgtggtgg
     2701 ccaagcgcaa cctgatcaag atcaagcgcg tcccggacat cctggtgtgg accacgatgt
     2761 cgccgatcat gttcgtgctg ctgttcggcc tggtgttcgg cagctcgatc cacatcccgg
     2821 gcgtgagcta ccgcgagtac ctgatcgcgg ggatcttcgc ccagacggtg atcttcgggt
     2881 cgaccttcac cggcgtcggg ctcgcggacg acatgacgaa gggcgtcatc gaccggttcc
     2941 ggtcgctccc gatggcccgc tcggcggtgc tcatcggccg gaccgcgagc gacctcgtct
     3001 acaactcgat ctcgatcctg gtcatgtccg tgaccggcct gatcgtgggc tggcgcatcc
     3061 ggaccggggt gctccccgcc atccgcgggt tcctgctgct gctggtcttc gcgtacgcga
     3121 tcagctggat catggcctgg gtcgggctaa aggtgagagc cgtggaggtg gtccagaacg
     3181 catcgttcat gttcatcttc ccggtgacgt tcgtggcgaa cacgttcgtc cggagcgaac
     3241 tgctgcccgg cccgctccag acgttcgccg aatggaaccc cgtctcggcg gtcgcccagg
     3301 gggtgcggga ggcgttcggg aacacgggca ccacgccgaa gccgaccgtc tggtcgctgg
     3361 cccacccgat cgcgtactcg ctgatctggg tggcgatcat cctggcgatc ttcgtgccac
     3421 tctcgatccg ccagttcagg aaggccgcat ccaggcagtg agggccaggg ctgggggccc
     3481 gcgaagcaga actgccacac tgccggcatg gctgacgttc cggctcgtga cctgcggaac
     3541 gacgtgagcg cggtgctccg ccgggtggag gggcgagagc gcctccgggt caccgtcagc
     3601 gggcgtccgg tcgccgagct ggttcccctg gcagggaggc ccgggtcgat ccgatgggac
     3661 gcctttctcg agaacgccga gcggtggcgg gcggatgcgg ggctcgcgaa ggatctcgcg
     3721 gcgctccttc ccgaaaccac cgacgacgtt ccctcgccgt gaccgggcgg cgcccacctc
     3781 gggccgagct cggcctcgcg gacacgtccc tgttcatcgc attcgaacag aaacgccacc
     3841 tcgccggctc cccgcctgag agcatcgcga tctcggtgat cacggtcggc gagctgcggc
     3901 tgggcgtgct ggccgcagac gatgggccga cgcgggcccg ccggctggag accctgtccc
     3961 gggccgaggc actcgagccc ctgcccgtcg acgcgctcgt ggcgcacgcg tgggcaacac
     4021 tgcgtctcgc gctccgcgac gcgggtcggc ggatgccgct caacgactcg tggatcgccg
     4081 cgacggccat cgcccacggt atcccggtcg tttcgcaaga cggcgactac gacggggtcc
     4141 ccggggtgga ggtcatccga gtctgaacgt cctcggcctt cgcgcgctga accgggcgct
     4201 gctggggcgc cagatgctgc tccggcggcg ccggctgggg gcgctggagg ccgtcgagcg
     4261 gctggtcgcg gtgcaggcgc aggttccccg ggatccgtac gtcgcgctgt ggtcgcggct
     4321 cgaggggttc cgcccctcgg tgctgtccgc ggcgctggaa ggccgccggg ccgtccggat
     4381 gaccctcctt cgggccacgc tccacctcgt gaccgcccgg gacgcggtgg ccctgcgccc
     4441 gctgttccag ccggtcgtgg agcggatgtt cttcagccag cgggcgttcc gccgcgctgc
     4501 cgagggagtc gacctcgacg aggtggtccg gctgttccgg gagctcctgg aggaacggcc
     4561 gggctcgagg gtcgctctcg cgcgagccgt ggccgagcgg tggcccgacc gcgacgcctc
     4621 gtcgctcggg tacgcgatgt acttcctgcc caccgtccag gtcacgccgc gaggcctgtg
     4681 ggggaagagc ggccgagccg cggtgacgac gctcgagcac tggctgggct cgggtccgct
     4741 cggaccgggc gcggcaccga gcgagctggt cgcgaggtac ctggctgtct tcggccccgc
     4801 gacgccggcc gacttctcga gctggtccgg cctggcgggc gtccgcgagg tgttcgagga
     4861 attccgcccg cgcctccgga cgttccgcga cgaacagggc cgagagctgt tcgacatccc
     4921 gcgcgcacca ctccccgatc ccgagacgcc cgccccggtc cggctcctgc ccgagtacga
     4981 caacgcgctg ctcggccacg ccgaccgcag cagggtcatc cccgagggga tcccgcagtg
     5041 gacggacgtc ggctggggca ccgtgctggt cgacggcttc atggcggcca gatggaagct
     5101 cgaccgggag caggtaggag cgacgttgcg ggtcgagccc tttcgccgcc tcacccgggc
     5161 cgagagggcc caggtcaccg aggagggcgt tcggctcgcc gagttcctcg aacccaccgg
     5221 acggcccggc gtgcaactcg ccgggcagat cgagccgtcc gcggccgagc gccgcccgaa
     5281 ttcgccgcgc aaccgatgag cgcctccatg tcgcggagtc gtttgcacga ccatcggggg
     5341 cggcgcagca tcgcggtcgt gggttcggtc gagtcaccgg tcagccggga acggcacgag
     5401 gtgccggatg gcgcctgcgg actcgtgacg gcgtcgagct cgacgcggac ctcgtcgtgg
     5461 tggcgaccgg cttcagccac gacccgttca cgccggacgt gcccggcagg gagacgttca
     5521 ccggccacca ggcgcactcg gccgactacc ggaatgcggg ccctaccggg gcaagaacgt
     5581 cctggtcgtg gggaccggga actccggcgc ggagatcgcg gtggacctgg tggagggcgg
     5641 cgccgcgaag gtggcgatct cggtccggac cccgcccaac atcctgcgcc gcgacctcgg
     5701 ggggattccg ttgcaggtcc ttgcggtgct catccgcagg ctgccggtcc gcgtggtcga
     5761 tcggatcgcc ctgatcctcc agcgcctcac cgtcggtgac ctctcccgct acggcatgcc
     5821 caatcccacg ctgggcctgt acacgcgggt caaccaggac gcgatcccga tcctggacgt
     5881 cggcctgatc gggatcgcgt cctggttgac ccgcgtgtac aggcccagcg tgggattggg
     5941 catgccgtag cgggagaggt caccgacggt gaggcgctgg aggatcaggg cgat
//
LOCUS       DASPWO010000041         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1109373, whole genome shotgun sequence.
ACCESSION   DASPWO010000041 DASPWO010000000
VERSION     DASPWO010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109373"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VEO00_01015"
     CDS             <1..302
                     /locus_tag="VEO00_01015"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421049.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HYU56613.1"
                     /translation="ESFERIHRSNLVGMGVLPLQFEEGQSVDALGLTGRELFAIRGLA
                     DGIEPGQRVAVEAVREDGTTVTLGATARIDGPADLEQYVAGGVLPMVARRLVAAR"
     gene            complement(315..866)
                     /locus_tag="VEO00_01020"
     CDS             complement(315..866)
                     /locus_tag="VEO00_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56614.1"
                     /translation="MDFGRGSRSEVARELPGDGLLPKARVTTRAITIRARPEHVWPWI
                     VQLGYGRAGWYSHDWIDNDGRPSADRIVPEAQQLEVGDRILLAPDMGPPVLEIVPNRF
                     LLAGDRYGGTFCFALFPHDEGTRMLSRTRTHWKPSLATLFWIAVVDPGAFVMERRMLL
                     GLKARAERRPAAPARPPRKPARD"
     gene            1088..2500
                     /locus_tag="VEO00_01025"
     CDS             1088..2500
                     /locus_tag="VEO00_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HYU56615.1"
                     /translation="MSSVATYPVHVEARLDPGLSRWLWLVKWFLAIPHYVVLAFLWIA
                     FVVLSVVAFFAILFTGRYPRAIFDFNVGVLRWSWRVSYYTFGAAGTDRYPPFTLREVP
                     DYPADLRIDYPNRLSRGLVLVKWWLLAIPHYLIVGLFVGGGAVVAYRSGEWVWAAGPG
                     LLGLLVLIALIGLAVTGRYLQSLFDLVLGLNRWALRVAGYAGLMTDEYPPFRLDLGGS
                     EPAGTLTVPKPPAPAGEPALPPAPGGRSGWTGARVTAVVIGALLALASLGLFAGGGVA
                     LWADLALRDGAGYVTSDAAAFSTETYALTSGDIALQAAPADVSVLRRVVGTVRVRVTP
                     DDPAAEVFVGIARSADVAGYLAGVSRAEIVDVPRGEARVTDGDAVPAPPAAQSFWAAR
                     SQGTGIRALTWASEAGRWTVVVMNADASAGLAIEADVGATAPGLLWIALGALGAGLIV
                     AIAAVFLLAVPIRRAGRVRP"
     gene            2497..3231
                     /locus_tag="VEO00_01030"
     CDS             2497..3231
                     /locus_tag="VEO00_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HYU56616.1"
                     /translation="MTRGSRLLEAIETREIDGADGEVLVPPLAVHARGREVNDVPTLT
                     RIDPREALGELLKPPRTPVLVTVPEMTFLMVDGTGDPNGEAFGEAVGALYGMSYTVKF
                     AVRGDEGIDVPVMPLEALWWMADGPALDPARRGDWRWTAMIAQPTLATPAMVEAARDR
                     VARKRPCPALEAVRLETFDEGRCAQVMHVGPYADEPATIAALHAFIAEQGLRPYGRHH
                     EIYLGDPRRTRPDRLRTILRQPVEPV"
     gene            3260..3559
                     /locus_tag="VEO00_01035"
     CDS             3260..3559
                     /locus_tag="VEO00_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HYU56617.1"
                     /translation="MNETLTEGTAAAAGTDVPDLRERAMVRLRKRREFAANVLAYVMV
                     NAFLVVIWAVTGAGYFWPVFPMAGWLIGLVFHGWDTFGSEPSEERIRREMERLGR"
BASE COUNT          453 a         1284 c         1420 g          526 t
ORIGIN      
        1 ccgagagctt cgagcggatc caccggagca acctggttgg catgggcgtg ctcccgctgc
       61 agttcgagga gggccagtcg gtggacgcgc tcgggctgac cgggcgcgag ctgttcgcga
      121 tccggggcct ggccgacggg atcgagccgg ggcagcgggt cgcggtggag gcggtccgcg
      181 aggacggcac gacggtgacc ctcggcgcga cggcccggat cgacgggccg gccgacctcg
      241 agcagtacgt cgccggaggc gtcctgccga tggtggctcg ccgcctggtc gcggcgcggt
      301 agcgtcgcgg cccgctagtc gcgcgccggc ttccggggag gccgggccgg cgccgccggt
      361 cgccgctccg cccgcgcctt cagcccgagg agcatccggc gctccatgac gaaggcgccg
      421 gggtcgacca cggcgatcca gaacagcgtg gcgagcgacg gcttccagtg cgtccgggtc
      481 cggctgagca ttcgcgtccc ctcgtcgtgc gggaagaggg cgaagcagaa ggtgccgccg
      541 tagcggtccc cggcgagcag gaaccggttc gggacgatct cgagcaccgg cgggcccatg
      601 tcgggcgcca gcaggatccg gtcgccgacc tcgagctgct gcgcctccgg cacgatccgg
      661 tcggcgctcg gccgaccgtc gttgtcgatc cagtcgtggc tgtaccagcc ggcccgcccg
      721 tacccgagct ggacgatcca cggccagacg tgctcgggcc gcgcacggat cgtgatcgcc
      781 cgcgtggtga ccctggcctt cgggagcagt ccgtctcccg gcagctcgcg cgcgacctca
      841 gaccgcgagc cccggccaaa gtccaagcca cgcggccagc gcgacggcga cggtggcgac
      901 ggcggcgacc acggccgcca cgagcagcat gatcgtcatc cgcgtccgca tccgccctcc
      961 tcggccggat cgcagcgtcg catcccgacg tggccagggc acggtccgga ggtcccgcga
     1021 accacgggac gtgaggcact cgggacgacc ggccggcgcc tctagcgttg gcggcgaggt
     1081 gatgacggtg tcgtctgtcg caacgtatcc ggttcacgtg gaggcccggc tcgaccccgg
     1141 gctgtcccgc tggctgtggt tggtgaagtg gttcctggcg atcccgcact acgtcgtgct
     1201 ggcgttcctg tggatcgcgt tcgtggtcct gtcggtggtc gcgttcttcg cgatcctgtt
     1261 caccgggcgc tatccgcgcg cgatcttcga cttcaacgtc ggggtcctcc gctggagttg
     1321 gcgcgtctcc tactacacgt tcggcgcggc cgggaccgac cgctatccgc cgttcacgct
     1381 tcgcgaggtc ccggactatc cggccgacct gcggatcgac tacccgaacc gcctgtcgcg
     1441 cggcctggtg ctggtgaagt ggtggctcct ggcgatcccc cactacctga tcgtcggcct
     1501 gttcgtcggc gggggcgccg tggtggcgta ccggtcgggg gagtgggtgt gggccgcggg
     1561 ccctggcctg ctcggcctgc tggtcctgat cgcgttgatc ggcctcgcgg tcaccggccg
     1621 ctacctgcag tcgctgttcg acctggtgct cgggctgaac cgatgggcgc tgcgggtggc
     1681 cgggtacgcc ggcctgatga ccgacgagta cccgccgttc cggctggacc tcggtgggtc
     1741 cgagccggcg gggacgctga cggtcccgaa gccgccggca cctgccggcg agccggcgct
     1801 gccgcccgcc ccgggcggcc gctcggggtg gaccggtgcg cgggtgaccg cggtggtgat
     1861 cggcgcgctg ctggcgctcg cgtcgctcgg cctgttcgcc ggcggcggcg tggccctgtg
     1921 ggccgacctc gccctgcgcg acggcgccgg atacgtcacg agcgacgcgg cggcgttctc
     1981 cacggaaacg tacgcgctga cctccggcga catcgcgctg caggcggccc cggccgacgt
     2041 gtcggtgctg cgcagggtcg tcggcacggt gcgcgtgcgc gtgacgcccg acgacccggc
     2101 ggcggaggtg ttcgtcggca tcgcccggag cgccgacgtc gccggctacc tggccggcgt
     2161 ctcccgggca gagatcgtcg acgtcccgag gggcgaggcg cgagtgacgg atggcgacgc
     2221 ggttccggcg ccgccggccg cgcagtcctt ctgggccgcg cggagccagg gcacgggcat
     2281 ccgcgccctg acctgggcca gcgaggccgg acgctggacg gtcgtggtca tgaacgccga
     2341 cgcgtcggcc gggctggcga tcgaggccga cgtgggcgcc acggcgcccg gcctgctctg
     2401 gatcgcactc ggcgcgctcg gggccgggct gatcgtggcg atcgcggcgg tgttcctgct
     2461 ggccgtcccg atccgccggg cggggagggt tcggccatga cgcgcggcag ccggcttctg
     2521 gaagcgatcg agacgcggga gatcgacggg gcggacgggg aggtcctcgt cccgccactg
     2581 gcggtccacg cacgaggacg ggaggtgaac gacgtgccta cgttgacgcg catcgacccg
     2641 cgggaggcgc tcggcgagct gttgaagccg ccgcggacgc ccgtgctggt gacggtcccc
     2701 gagatgacgt tcctgatggt ggacggaacc ggcgatccga acggcgaggc cttcggggag
     2761 gccgtcgggg cgctctacgg gatgtcctac accgtgaagt tcgcagtgcg cggtgacgag
     2821 ggcatcgacg tcccggtcat gccgctcgag gcgctgtggt ggatggccga cggcccggcc
     2881 ctggaccccg cccgccgggg cgactggcgc tggaccgcga tgatcgccca gccgacgctc
     2941 gcgaccccgg ccatggtgga ggccgcccgc gaccgcgtgg cccgcaagcg cccgtgcccg
     3001 gccctggagg cggtccgcct ggagacattc gacgaggggc ggtgcgccca ggtgatgcac
     3061 gtcggcccgt acgcggacga gccggcgacg atcgcggcgc tgcacgcgtt catcgccgag
     3121 caggggctgc gcccgtacgg gcgccaccac gagatctacc tcggcgaccc gcgacggacc
     3181 cgtcccgacc gcctgcgcac gatcctgcgc cagccggtcg aacccgtgta agcgttcgga
     3241 aaggaaggga gtgatcggga tgaacgagac cctgaccgag ggaacggccg ccgccgccgg
     3301 cacggatgtg ccggacctcc gggagcgggc gatggtgcgg ctgcgcaagc gccgcgagtt
     3361 cgccgccaac gtgctggcct acgtgatggt gaacgcgttc ctggtcgtga tctgggccgt
     3421 gaccggcgcc ggctacttct ggccggtgtt cccgatggcg ggctggctga tcggcctggt
     3481 cttccacggc tgggacacgt tcgggtcgga gccgagcgag gagcgcatcc gccgggagat
     3541 ggagcgcttg ggccgctgaa gggaccgcgc ggactacgcg gagaggtcgc ggcgctcgaa
     3601 cgcggcgagc cccactacca ccgccaccgc ggcgatcgcc accagcacca tgacgtgcag
     3661 cgggtcgagc ccgttcagga tcg
//
LOCUS       DASPWO010000042         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_939588, whole genome shotgun sequence.
ACCESSION   DASPWO010000042 DASPWO010000000
VERSION     DASPWO010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939588"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /gene="trpB"
                     /locus_tag="VEO00_01040"
     CDS             complement(<1..985)
                     /gene="trpB"
                     /locus_tag="VEO00_01040"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HYU56618.1"
                     /translation="MSAVLTGRPDARGRFGAFGGRYVPEVLIPALDELTGAWETLRDD
                     PAFRGELLELLRDYVGRPTALTHAGRLSDELGYRIWLKREDLAHTGAHKINNTVGQVL
                     LARRLGKARIIAETGAGQHGVATATACALFGLPCVVYMGEEDTRRQAPNVARMRLLGA
                     EVVTVTSGSRTLKDAISEALRDWAASVRDTHYVVGSVVGPHPFPALVRDLQRVIGDEA
                     RSQFRAATGGDPDVVAACVGGGSNAIGTFTAFVGSGADLVGIEAGGRGDRRGEHCASL
                     SLGRPGVLHGALSYLLQDEHGQVEATHSISAGLDYPGVGPEHAWLKEQRLAR"
     gene            complement(996..1784)
                     /locus_tag="VEO00_01045"
     CDS             complement(996..1784)
                     /locus_tag="VEO00_01045"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HYU56619.1"
                     /translation="MGFLTRLVDDLRHSLAERPMPEGLLMARARAMPPPRPFAAALST
                     PGLAVIAEVKRASPSAGAIAEVDAGAQAERYGRAGAAAVSVLTEPRHFDGSLADLRLA
                     RRRTSLPLLRKDFLVHPAQVAESRVEGADAILLIAAALPGSELADMLEAAEDTEVEAL
                     VEVHDEDEVARAIEAGARVIGVNARDLETLRVDAERALDVARAVPPDRTVVLESAIAT
                     GEQARRAEEAGADAILVGEALMRAADPAALLAELVGRPVRAAAR"
     gene            complement(1853..2281)
                     /locus_tag="VEO00_01050"
     CDS             complement(1853..2281)
                     /locus_tag="VEO00_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56620.1"
                     /translation="MQRSSGPGVVTGLGGALAVIGAFLAWAKVTAAPGVSVSFAGTDE
                     AIDPISGWYALIAGVIVLLVAVAMFAQGARPWHGIVALLGGAAILLIAIIDIVNVEEG
                     VGIGIGLWITIVGGALAVVGGIWGLRGGMRAGPAMRPPAA"
     gene            complement(2335..>2901)
                     /locus_tag="VEO00_01055"
     CDS             complement(2335..>2901)
                     /locus_tag="VEO00_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56621.1"
                     /translation="GRGADRCRRRRDGPVPRNVLDTPVRWGGALAAGGGIVAALSVLL
                     LPWATVSIPSRGLHQTVFVRSGSDGVVVLALAGLLIAVGGLLLIGRAKRAAAVVAVAA
                     GLLLAAWGIGEVLLARDSVLDAYAEDVAAELHSPVQEVRSTLETVEIAPSAGVFGSVL
                     AGAVGLMGGFAGILAGRKDRDPSRSGRH"
BASE COUNT          425 a         1145 c          999 g          332 t
ORIGIN      
        1 agcgcgccag ccgttgctcc ttcagccagg cgtgctccgg cccgaccccc gggtagtcga
       61 gcccggcgga gatcgagtgg gtcgcctcga cctgtccgtg ctcgtcctgc agcaggtagg
      121 acagcgcgcc gtgcaggact cccggccggc cgagcgagag cgaggcgcag tgctcgccgc
      181 ggcgatcgcc ccggccgccg gcctcgatcc cgaccagatc ggcgcccgag ccgacgaacg
      241 cggtgaaggt cccgatggcg ttcgacccgc caccgacgca ggcggccacg acgtccggat
      301 cgccgccggt ggccgcgcgg aactgcgaac gggcttcgtc gccgatcacg cgctgaagat
      361 cgcggacgag cgcggggaac gggtgcggcc cgaccaccga ccccacgacg tagtgggtgt
      421 cgcgcaccga cgcggcccag tcgcgcagcg cctcgctgat cgcgtccttc agcgtgcgcg
      481 agccggacgt gacggtcacc acctcggcgc cgagcaggcg catcctggcg acgttcggtg
      541 cctgccgccg cgtgtcctcc tcgcccatgt acacaacaca cggcaggccg aacagcgcgc
      601 aggccgtcgc ggtggccacc ccgtgctggc cggcgccggt ctcggcgatg atccgcgcct
      661 tccccaggcg gcgggcgagc agcacctgcc cgaccgtgtt gttgatcttg tgcgccccgg
      721 tgtgagcgag gtcctcacgc ttcagccaga tccggtagcc gagctcgtcg gacaggcgac
      781 cggcgtgggt cagcgccgtg gggcgaccga cgtagtcgcg cagcaactcc aggagttccc
      841 cgcggaacgc cgggtcgtcg cgcagcgtct cccaggcccc cgtcagctcg tcgagggcgg
      901 ggatcaggac ctcggggacg tagcggccgc cgaaggcccc gaaccggccc cgcgcgtccg
      961 gccgcccggt caggacggcg ctcacccggt cggcctcacc gcgcggcggc ccgcaccggc
     1021 cgcccgacca gctccgccag cagggcggcg gggtcggccg cccgcatcag ggcctcgccg
     1081 acgaggatcg cgtccgcccc ggcctcctct gcccgccgcg cctgctcgcc ggtggcgatc
     1141 gcgctctcga gcacgacggt ccggtccggc gggacggcgc gggcgacatc gagggcccgc
     1201 tccgcgtcca ctcgcagcgt ctccaggtcc cgcgcgttca cgccgatcac gcgcgccccg
     1261 gcctcgatcg cgcgggccac ctcgtcctca tcgtgcacct ccaccagcgc ctcaacctcc
     1321 gtgtcctcgg ccgcctccag catgtcggcg agctcggagc cggggagcgc cgccgcgatc
     1381 agtaggatcg cgtcggctcc ctcaacccgc gactcggcca cctgcgccgg gtggaccagg
     1441 aagtccttgc gcaacagggg caacgacgtc cggcgcctgg ccaggcggag gtccgcgagc
     1501 gaaccgtcga aatggcgcgg ctcggtcagc accgacaccg cggccgcccc ggcgcgcccg
     1561 taacgctcgg cctgggcacc ggcgtcgacc tcggcgatcg ccccggccga cggcgaggcg
     1621 cgcttcacct cggcgatcac ggccaggccc ggagtcgaga gcgccgccgc gaacggtcga
     1681 ggtgggggca tggcgcgcgc gcgggccatc aggaggccct cgggcatcgg gcgctcggcc
     1741 aggctgtggc gcaggtcgtc gacgaggcgg gtcaggaacc ccaagggacg ccgagtatag
     1801 gtccggcccg gcggtggccg ggccggacct ccgtgcctcg ttcgcctgct gcttacgccg
     1861 cgggcggccg catggcgggc cccgccctca tgccgccgcg gaggccccag atcccgccga
     1921 cgacggccag agcaccgccg acgatcgtga tccagagtcc gatgccgatc ccgaccccct
     1981 cctccacgtt gacgatgtcg atgatcgcga tgagcaggat ggccgcacct ccgagcaggg
     2041 cgacgatgcc gtgccagggc cttgcgccct gcgcgaacat cgcgaccgcc accagcagga
     2101 cgatgacgcc cgcgatgagc gcgtaccacc cggagatcgg atctatcgct tcatcggtgc
     2161 cagcgaagct gacactcacc cccggcgccg cggtgacctt cgcccacgcc aggaacgcgc
     2221 cgatcaccgc gagcgcccca ccgagaccgg tcaccactcc cggaccactc gatctctgca
     2281 ctgcccctcc ccccttccag gacggctgca ttctcggcgc gagaacgagc ggagtcaatg
     2341 acggccagag cgcgaaggat cacggtcctt ccggccggca aggatcccgg cgaagccgcc
     2401 catgaggccg accgcgcccg ccaagaccga cccgaagacg cccgcggagg gtgcgatctc
     2461 cacagtctcg agcgtggacc ggacctcctg caccggggaa tggagctccg ccgcgacgtc
     2521 ctccgcgtag gcgtccagga cgctgtcacg cgccagcagc acctcgccga tcccccaggc
     2581 ggcgaggagg agtccggcag ccacggcgac gaccgcggcg gcccgcttcg cccggccgat
     2641 cagcaggagc cctccgaccg cgatcaagag gccggccaga gcgaggacga cgacgccatc
     2701 ggagcccgac cgtacgaaca ccgtctggtg cagtccccgc gacgggatgg ataccgtcgc
     2761 ccacggcagc agcagcactg acagcgcggc gacgatgccg ccgcccgccg cgagagcccc
     2821 tccccacctc accggggtgt cgaggacgtt ccgcggaaca ggcccatcgc gccgccggcg
     2881 acaccgatca gcgccccggc c
//
LOCUS       DASPWO010000043         3971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1029213, whole genome shotgun sequence.
ACCESSION   DASPWO010000043 DASPWO010000000
VERSION     DASPWO010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029213"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VEO00_01060"
     CDS             complement(<1..772)
                     /locus_tag="VEO00_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU56622.1"
                     /translation="MICPNCGSSIPDGARFCPACGTPLPTAVGIEERKIVTVLFADLV
                     DSTGLAQRLDPERAREVISAFYDLASQELASLRGRAEKFIGDAVMAVFGLPVVHEDDA
                     VRAVRAALQIRERTQRLGEELGLQLETHAGLASGDVAVGKGPAGQLLVTGSTVNLAAR
                     LQEEAASGETLLAETTHLLTAESVEVAGRRAVGVGGFDGTVAAYPVVRIVPRRTRRTI
                     PLVGRRHEVNLLRDTFDRAVQSKRPHLFTLLGEPGIGKT"
     gene            836..1324
                     /locus_tag="VEO00_01065"
     CDS             836..1324
                     /locus_tag="VEO00_01065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU56623.1"
                     /translation="MATSAGGLTIRRARPEEAEALSELAAWSKAVWGYDEAFLEEARQ
                     LLRITPEYVARAEVHVAEDGGRLVGFYGLERAGGAEAEVGFLFVDPDALGTGVGRALW
                     DHAVGTARALGLTSFLIEADPHAEGFYRTMGAEWIGTRPTGVGGRPLPLFRFSIGEGG
                     RR"
     gene            1321..1716
                     /locus_tag="VEO00_01070"
     CDS             1321..1716
                     /locus_tag="VEO00_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYU56624.1"
                     /translation="MTEPGEVVRGVWERFEARDWDEAADLLAEDLVIDWPNTGERFRG
                     RDNFIAMNRNHPAADWHIQVRRIVASGPDVAAEVLVPHADGLDACLGFYEVADGRITT
                     GREYWVEIRPQSIPDWRAAWTEPIPTNGP"
     gene            complement(1721..1906)
                     /locus_tag="VEO00_01075"
     CDS             complement(1721..1906)
                     /locus_tag="VEO00_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56625.1"
                     /translation="MDEATAAEEAVPPLPAPVKAAVWAAMLFPAAAALTVALWKLRRR
                     LREQLDRSEKKRRAASA"
     gene            complement(1906..2820)
                     /locus_tag="VEO00_01080"
     CDS             complement(1906..2820)
                     /locus_tag="VEO00_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HYU56626.1"
                     /translation="MTVLSLPERVTIREVGPRDGLQAEKPLPVGDRVRLIDALSATGV
                     RKIEAVSFVSPRAVPAMADAERVWAGISRAPGVAYSALVPNRIGAEKASEAGVDALKA
                     FLAASDDYNRKNVNRSVEESIADVAEVVEVGRAAGIPVEVAISAAFGDPYEGDVPPER
                     VARVAARLVEVGCVGISLGDTTGMATPVRLVQVLDHLRERVPGVPLNLHLHDTRGTAM
                     ANALAGLQAGITDFDSSVGGMGGSPFAPGANGNVATEDLVYMLEDMGVATGVDLAAMI
                     ATAKLAEDLLGHALPGQIHVAGPRPGGE"
     gene            complement(2825..3601)
                     /locus_tag="VEO00_01085"
     CDS             complement(2825..3601)
                     /locus_tag="VEO00_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYU56627.1"
                     /translation="MSTVERTRRGRVAIVTLSRPEALNAISGRVGDDLRDAFLAVAAD
                     REAWVMVLAAAGEKAFCVGADLKERSAFSKDDLQRNRHHIVDMFAALRAVPQPTIAAV
                     FGFALGGGFELALSCDLIVAADDARLGLTEVRVGLVPGGGGTQMLARIAGPARAKDLI
                     FTGRQVDAAEALAMGIVARVVPRAELEAATIALAEQIAGNAPIAVREAKRAVDRGLEV
                     PVEQGIAVEDVSWRRSVATADRAEGIAAFVEKRPPAWKGE"
     gene            complement(3776..>3971)
                     /locus_tag="VEO00_01090"
     CDS             complement(3776..>3971)
                     /locus_tag="VEO00_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56628.1"
                     /translation="GACRAHAANKYFMSASDADSLRAHPGCNCRVLKQPITSARFTEL
                     FQGSNGQLRTTFDARATLGG"
BASE COUNT          552 a         1496 c         1396 g          527 t
ORIGIN      
        1 tggtcttccc gatgccgggc tcaccgagga gcgtgaacag gtgggggcgc ttcgactgca
       61 cggcccggtc gaacgtgtcg cgcaggaggt tcacctcgtg ccgccgcccg acgagcggga
      121 tggtgcgccg ggtgcgccgc ggcacgatgc gcaccaccgg gtaggcggcc accgtcccgt
      181 cgaacccgcc gacgcccacc gcgcggcgtc cggccacctc caccgactcg gcggtcagca
      241 ggtgggtggt ctcggcgagc agggtctcgc cggacgcggc ctcctcctgc aggcgcgcgg
      301 cgaggttgac cgtcgagccg gtgaccagca gctgcccggc cgggcccttc ccgacggcca
      361 catcccccga ggccagcccg gcgtgggttt cgagctggag cccgagctcc tcgccgagcc
      421 gctgcgtgcg ctctcggatc tggagcgccg cccgcactgc ccgaaccgcg tcgtcctcgt
      481 ggacgaccgg cagaccgaag acggccatca ccgcgtcccc gatgaacttc tcggcccgcc
      541 cgcgaagcga ggcgagctcc tgtgaggcga ggtcgtagaa ggccgagatc acctcgcggg
      601 cgcgctccgg gtccagccgc tgcgcgagcc cggtcgaatc caccaggtcg gcgaacagga
      661 ccgtgacgat cttgcgctcc tcgatcccga cggccgtggg caacggcgtc ccgcacgccg
      721 gacagaagcg cgcaccgtcc gggatcgacg agccgcaatt ggggcagatc acgcgacgat
      781 tatcccagcg cggggtgaca cctcagcccc ccggcgacgg tggcaggatg tgaggatggc
      841 cacatccgcg ggagggctga cgatccgccg ggcgcggccc gaggaggccg aggcgctgtc
      901 ggagctcgcc gcgtggtcga aggcggtctg gggctacgac gaggcgttcc tcgaggaagc
      961 gcgccagctg ctgcggatca ctccagagta cgtcgcgcgg gccgaggtcc acgtggctga
     1021 ggacgggggc cggctggtcg gcttctacgg cctggaacgc gccggcggcg ccgaggccga
     1081 ggtggggttc ctgttcgtcg accccgatgc gctgggcacc ggcgtgggcc gcgcgctgtg
     1141 ggaccacgcg gtcgggaccg cccgggccct ggggctcacg tcgttcctga tcgaggcgga
     1201 cccgcacgcc gagggcttct accgaacgat gggcgccgaa tggatcggca cgcggccgac
     1261 cggggtcggc ggccgtccgc tgccgctctt ccgattctcg atcggcgagg ggggtcggcg
     1321 atgaccgaac cgggcgaggt ggtccgaggc gtctgggagc ggttcgaggc ccgcgactgg
     1381 gacgaagccg ccgatctgct ggccgaggac ctggtcatcg actggccgaa caccggggag
     1441 cgcttccgcg gccgcgacaa cttcatcgcc atgaaccgca accaccccgc ggccgactgg
     1501 cacatccagg tccgtcggat cgtggcgagc ggccccgacg tggccgccga ggtgctcgtc
     1561 ccccacgccg atggtctcga cgcctgcctc ggcttctacg aggtcgccga cggccggatc
     1621 accactggcc gtgagtactg ggtcgagatt cggccccaat ccatccccga ctggcgagcg
     1681 gcctggaccg agccgatccc gaccaacggc ccttgagccg tcatgcggac gcggcccgac
     1741 gcttcttctc ggaccggtcc agctgctcgc gcaagcggcg gcggagcttc cacagggcga
     1801 ccgtcagggc cgcagccgcg ggaaacagca tggccgccca caccgccgcc ttcaccggcg
     1861 ccggaagagg ggggacggcc tcctccgccg ccgtcgcctc gtccatcact ctcccccggg
     1921 gcgggggccg gcgacgtgga tctggccggg cagggcgtga ccgagcagat cctccgcgag
     1981 cttcgccgtg gcgatcatcg ctgcgaggtc caccccggtc gccacgccca tgtcctccag
     2041 catgtagacc aggtcctcgg tggcaacgtt gccgttcgcc ccgggggcga acggcgagcc
     2101 gcccatcccg ccgacgctcg agtcgaagtc cgtgataccg gcctgcaggc ccgcgagggc
     2161 gttcgccatc gcggtgccgc gcgtgtcgtg caggtgcagg ttgagcggca cgcccggcac
     2221 ccgttcccgc agatggtcaa ggacctgtac caatcgaacc ggggtggcca tcccggtcgt
     2281 gtcgccgagc gagatgccga cgcacccgac ctccaccagc cgcgcggcga cccgcgccac
     2341 ccgttccggc ggcacgtcgc cctcgtacgg gtcgccgaac gccgccgaga tcgcgacctc
     2401 caccgggatg ccagcggcgc gccccacctc cacgacctcc gccacgtccg cgatcgactc
     2461 ctcgacggag cggttcacgt tcttgcggtt gtagtcgtcg gacgcggcga ggaacgcctt
     2521 cagagcgtcc acgcctgcct ccgacgcctt ctcggccccg atccggttgg ggacgagcgc
     2581 ggagtaggcg acgcccggcg ccctgctgat ccccgcccac acccgttcgg cgtcggccat
     2641 cgccggcacc gcccgcggcg agacgaacga gaccgcctcg atcttgcgca ccccggtcgc
     2701 ggacagcgcg tcgatcaggc ggacgcggtc cccgaccggc agcggcttct ccgcctgcag
     2761 cccgtcccgc ggcccgacct cacggatcgt cacgcgctcc ggaagggaga ggaccgtcac
     2821 gccgttactc gcccttccag gccggggggc gcttctcgac gaaggccgcg atgccctcgg
     2881 cgcggtcggc cgtggcgacc gagcggcgcc aggacacgtc ctcgaccgcg atcccctgct
     2941 ccacgggcac ctcgagcccg cggtcgacgg cgcgcttcgc ttcccgcacg gcgatcggcg
     3001 cgttgccggc gatctgctcg gcgagcgcga tcgtggcggc ctcgagctcg gcccggggaa
     3061 cgacccgggc gacgatcccc atcgccagcg cctcggcggc gtccacctgg cggccggtga
     3121 agatcaggtc cttcgcccgc gccggtcccg cgatccgcgc gagcatctgc gtcccgcccc
     3181 cgcccggcac caggccgacg cgcacctcgg tcagccccag gcgagcgtcg tcggcggcga
     3241 cgatcaggtc gcacgacagc gccagctcga agccgccccc cagcgcgaac ccgaacacgg
     3301 ccgcgatggt gggctgcggc acggcgcgca gcgccgcgaa catgtcgacg atgtggtggc
     3361 ggttgcgctg caagtcgtcc ttggagaacg cggaacgttc cttcaggtcg gcaccgacgc
     3421 agaacgcctt ctcgcccgcg gccgccagta ccatcaccca ggcctcgcgg tcggcggcca
     3481 ccgccaggaa cgcgtcccgc aggtcgtcgc ccacccgtcc ggagatggcg ttcagcgcct
     3541 ccgggcgcga cagcgtgacg atcgccacgc ggcctcgccg ggtccgctcg acggtgctca
     3601 cggcgcggag tctacggtgg catcgcgagc ggacgacgtg cgaaggcccc gaggaacggg
     3661 agagccctct ggaggggagg gagactcagg acgaaacccg gccaccccgg tctgcgcgca
     3721 acagcggctg acctcccttt cgggcccctt ccggaaggct ctcggcgctc ctctcctatc
     3781 cgcccagcgt ggcccgggcg tcgaacgtgg ttcgcaactg accgttcgat ccctggaaca
     3841 gctccgtgaa tcgcgcactc gtgatcggct gcttcagcac ccggcagttg cagccggggt
     3901 gggctcgcag gctgtccgca tcggacgcgc tcatgaagta cttgttcgcg gcgtgcgccc
     3961 ggcaggctcc g
//
LOCUS       DASPWO010000044         8016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_686633, whole genome shotgun sequence.
ACCESSION   DASPWO010000044 DASPWO010000000
VERSION     DASPWO010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686633"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="VEO00_01095"
     CDS             complement(<1..1377)
                     /locus_tag="VEO00_01095"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HYU56629.1"
                     /translation="MFDSSGPSFVHLDVRSYFSLKDGAFSPEELAARAAELSMPAVAL
                     TDRDGLYGAPRFAAACARTGVRPIFGTTLTIRGPGPAPARPADTSVVLLARDARGYAN
                     LCRLITDAHLGGERGDPHAFVAQILAHADGLACLLGPRSAPGLLAAAGRAEEAAAAAR
                     PYREAFGRWAFVSVEHRLEEDAHREIRGMLRLAGDAGLRALATNGVRYLVPEDAFLAD
                     ALECMRELVPLASHHVSRRNAEGYLKDARRMRALFAERPGLCDAAVALAEACDVDLGI
                     GTVHFPDFPTPRGKSAGSVLAERCRRGMEDRGVRPTREVVDRLNHELSMIQTMGYAAY
                     FLTVADIVTDVRAMGIRIACRGSAAGSLVCYLTRISDVDPVRHGLLFERFINPGRDDL
                     PDIDVDVESARREDVYDMILSRYGEDRCACVNMVETYRARAALREVGKALGYPEVEID
                     VVAKAFPHI"
     gene            1459..1836
                     /locus_tag="VEO00_01100"
     CDS             1459..1836
                     /locus_tag="VEO00_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYU56630.1"
                     /translation="MKVATVGWEVARQRNRAGLTQAELASRMGTTQSAVSKIESGRVI
                     PTLPWLERFARAIDRPFDLRIQPSKAPSRAMKRARVRQVLGEYRFNPWARDPSPAEAR
                     TLLADGLTRERFERSHAAPAGKR"
     gene            1790..2386
                     /locus_tag="VEO00_01105"
     CDS             1790..2386
                     /locus_tag="VEO00_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56631.1"
                     /translation="MSDSNARTLLQQASAERARLARHLLVAAALREILSTDPIIVGGT
                     AEEYWTEDEYHETDLDLCAPITRTDEASLGRLGFSKEGRHWVHPRINVAVEFPGSRID
                     GDASRTVEVPTSDAHARVIGVDDLYLDRLRQATIHERVEGIEFKSALAVTSACYDQID
                     WEYVEDRLAAIIREEGDVGASMKRIDARIRRRVRRQIR"
     gene            complement(2407..2667)
                     /locus_tag="VEO00_01110"
     CDS             complement(2407..2667)
                     /locus_tag="VEO00_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56632.1"
                     /translation="MRTGTRIAVGAGAAGALYLIVRRAVRDARRPGRGYRGNAVRVGL
                     AMAALYAAAWVWLHLAAVLLVVVSLAGLVLASRGRRRPAGRH"
     gene            complement(2807..3052)
                     /locus_tag="VEO00_01115"
     CDS             complement(2807..3052)
                     /locus_tag="VEO00_01115"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HYU56633.1"
                     /translation="MSKIYDELVTVERDAGMPAAFAWRGRRYEVANVIGRWRIEGRWW
                     EDGRDREYWRVEAKGGAVCDLYHDRRAGRWHLERIWD"
     gene            complement(3157..4128)
                     /locus_tag="VEO00_01120"
     CDS             complement(3157..4128)
                     /locus_tag="VEO00_01120"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HYU56634.1"
                     /translation="MTPLAAAAIALAVAAAAGFGARWLGLATGALDFPTGRLKPHPQP
                     VSYLGGLAVAAGIAAGLAARAWPLPWFLTVALFGIALVGLADDVLGFPPVVRLGAQVA
                     LGALVAANGLDLPPLGAVLGWLAAMVFFAGAVNAVNMVDGMDGLAGSAAALSGIGLAV
                     VAALDDRGTLLPLLLAGASLGFLFHNRPPARLFLGNSGSYLAGATLAVAVLLLAGGPA
                     LMVGAGTCLGLFALDLLLSVARRVAGRSALLEPDREHLYDQLLGRGFSVVECLVICAV
                     VHALLVGAGVGISRMPSGLAVASAAGLWVVALGALIGLGFVGRRARY"
     gene            complement(4125..5324)
                     /locus_tag="VEO00_01125"
     CDS             complement(4125..5324)
                     /locus_tag="VEO00_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYU56635.1"
                     /translation="MGTGRPVRLLHLTTVDLSLAALLLNQLERYGKEGYAVTAVSAPG
                     PHVRELEEHGIRHIPIPSLTRSWTPLQDLRALLALRRLFRRERPDIVHTHNPKTGVLG
                     RIAARLAGVPIVVNTVHGSYAPADLGTVKRSLVRLADRVSSRVSDFELFQSAEDHRDA
                     VARGVVPARRAAWLGNGVDTARFGPDAVLMERVSALRSKWERAAGPETSRRFVVGMVG
                     RLVREKGYVEFFRAADVVRRARPDALFVAVGMEDPAKTDRLSPEVLARVRAGESVVLA
                     GEAAGADMPAVYRAFDLFALPSYREGVPRSAIEAMASARAVVATDVRGCREVVADGET
                     GLLVPARDHGALARAILRLVEDDVLRTEMEAAGRRRVALRFDEERVIERTLAVYERLL
                     RDRGRRG"
     gene            complement(5324..6151)
                     /locus_tag="VEO00_01130"
     CDS             complement(5324..6151)
                     /locus_tag="VEO00_01130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYU56636.1"
                     /translation="MGVRFALVSVAKRAVKLALLPAAYLRREREPGLFVLIYHRVGAR
                     MGEEMDLPEARFTEQMRLLRERYTVVPLRAGLERVEASAVDRDLVAVTFDDGYQDVFA
                     RAWPVLSAFEIPATLFLATGFLDGTTPAPLSHPRGRGEPPRPLAWDAIHQMQQTGLVE
                     VGSHSVTHPDFDRIDGARAERELAESKRVIAERLGTEPVVFAYPRAVVGHQDLVTRHY
                     RWAVGGDRGKNVPGGVDRGRVQRTPVRRSDGTFFLRRRLDGIAPLEDRLYARMKWTR"
     gene            complement(6106..7512)
                     /locus_tag="VEO00_01135"
     CDS             complement(6106..7512)
                     /locus_tag="VEO00_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56637.1"
                     /translation="MATASPTFTGSNRALSRMVWTLIAGLVVLAAWVMGSMPLEIQSY
                     VAFAGLTLVAGSLILPVAWRKDRTISLKVLFLALAVFIIGALIRFLVTQMFYGGVSDA
                     RGYFTNGVSLAEDFRVGNYSALIPPYTDTNAINYAAGFLYTLIPPSMLAGFVVFAGMA
                     FIGSWHFYKAFRVSFPDGDRRLYAIFIFFLPSFWYWTTSLGKDAPMLMGLGVATYGFA
                     LMFRKLSAKAVAYVVVGLGVVSMVRSPLAAALAVAGVAAFALRPTRARTSHVQALAYL
                     LLIPILALGALTLIAKGRQFVGATEGTSIQSVVAQAQQQDFDSDEGSNFSPASPFTPQ
                     GLALALITANFRPFPWEAGGLLPALQSLEGVGILMLFLLKFRAIGRVLRAWRRNAMTI
                     MVLLSTVFISIVLTSLANFGLLARQRTQVLPFLLMIPCMMVVQSLRPGPKPEEEQADR
                     PVPAWASGSRSSLSRSAR"
     gene            complement(7525..>8016)
                     /locus_tag="VEO00_01140"
     CDS             complement(7525..>8016)
                     /locus_tag="VEO00_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU56638.1"
                     /translation="SALRPAAEALQGAVAERVLARAVRPRHALATPRTPAYLRWRYGA
                     GPIPYHALLDGDALVILRVRERGPLAEAVVDEVLAPDAAAARRGLSRAVRASGAHHAV
                     AHFGQEWPARVALARAGFAPLPGQGMIFVVRPVGAAGGRGSGPDPLRAASWSLSLGDL
                     EVF"
BASE COUNT         1267 a         2963 c         2809 g          977 t
ORIGIN      
        1 gatgtgcggg aacgccttcg ccaccacgtc gatctcgacc tcggggtagc cgagggcctt
       61 cccgacctcg cgcagcgccg cgcgcgcccg gtaggtctcg accatgttca cgcaggcgca
      121 gcggtcctcg ccgtagcgcg acaggatcat gtcgtagacg tcctcgcgcc gggccgactc
      181 gacgtcgacg tcgatgtcgg gcaggtcatc gcgtcccggg ttgatgaatc gctcgaacag
      241 caggccgtgg cggaccgggt cgacgtccga gatgcgggtc aggtagcaga cgagcgaccc
      301 tgcggcggac ccgcggcagg cgatgcggat gcccatcgcc cggacgtcgg tcacgatgtc
      361 ggcgaccgtc aggaagtacg ccgcgtagcc catcgtctgg atcatcgaga gctcgtggtt
      421 cagccggtcg acgacttccc gcgtcggtcg caccccgcgg tcctccatcc cccgccggca
      481 gcgctcggcg aggaccgagc cggcgctctt cccgcgcggg gtcgggaagt ccggaaagtg
      541 gacggtcccg atgccgaggt cgacgtcgca cgcctcggcc agggcgaccg cggcgtcgca
      601 gagcccgggg cgctcggcga acagcgcgcg catccgccgg gcgtccttca ggtagccctc
      661 ggcgttccgc cgcgagacgt ggtgcgacgc gagcgggacg agctcgcgca tgcactcgag
      721 cgcgtcggcc aggaacgcgt cctccggcac caggtagcgc acgccgttcg tcgccagcgc
      781 ccgcaggccg gcgtcgccgg cgaggcggag catcccccgg atctcgcggt gagcgtcctc
      841 ttccaggcgg tgctccaccg agacgaacgc ccaccgtccg aacgcctcgc ggtagggccg
      901 cgccgccgcg gcggcctcct ccgcgcgccc ggccgcggcc aagaggccgg gggccgaccg
      961 gggaccgagc aggcaggcca gcccgtcggc gtgcgccagg atctgggcga cgaacgcgtg
     1021 cgggtccccg cgctcgccac ccaggtgggc gtcggtgatc agccggcaga ggttcgcgta
     1081 ccccctggcg tcccgcgcca gcagcaccac gctcgtgtcg gccggccgcg ccggcgccgg
     1141 tccgggtccg cggatcgtca gggtggtccc gaagatcggt cgcacgccgg tcctggcgca
     1201 ggcggcggcg aagcggggcg ccccgtacag cccgtcgcgg tcggtcagcg cgaccgccgg
     1261 catcgatagc tccgccgccc gcgccgccag ctcctccggc gagaacgccc cgtccttcag
     1321 cgagaagtac gaccgaacgt cgaggtgcac gaaggatggc ccagaggagt cgaacacatg
     1381 ttcgatccta ggcgagccgg gttcggtctg tccagaggga tgctgatatt gctaacttgc
     1441 tatatactcc cccaaagcat gaaggtcgca acggtcggat gggaagtcgc ccgccagcgg
     1501 aatcgagctg gcctcaccca ggccgagctc gcttcacgga tgggcaccac gcaatcagcc
     1561 gtctcaaaga tcgagtcggg acgcgtgatc cctacccttc cctggctcga gcggtttgcc
     1621 cgtgccatcg atcgaccttt cgacctgcgc atacagccgt ccaaggcacc gagtcgggcg
     1681 atgaagcgtg ctcgggttcg tcaggtcctt ggcgagtatc gattcaatcc ctgggctcgg
     1741 gatccgagtc ctgctgaggc tcgcaccctc cttgcggatg ggttgacccg tgagcgattc
     1801 gaacgctcgc acgctgctcc agcaggcaag cgctgagcgg gccagactcg ctcgacatct
     1861 cctcgttgcc gccgcgcttc gggagatcct gtccacggat ccgatcatcg tcggcggtac
     1921 cgccgaggag tactggacgg aggacgagta ccacgagacc gatctcgatc tgtgtgcccc
     1981 gatcacccgc accgacgaag cctccctggg acgactggga ttctcgaagg agggacggca
     2041 ttgggtccat ccccggatca acgtcgccgt cgagttcccc ggctctcgta tcgacggcga
     2101 tgcgagcaga accgtcgaag tgccaacgag cgatgctcac gctcgggtga tcggggtcga
     2161 tgacctctat ctcgatcggc tacggcaggc cacgatccat gaacgggtgg agggcatcga
     2221 gttcaagtcc gcactggcgg tgacgtccgc ctgctatgac cagatcgact gggagtacgt
     2281 cgaggatcga ctcgcagcca tcattcgcga ggagggggat gtcggagcgt cgatgaaacg
     2341 gatcgatgca cggatacggc gccgggtcag acgacagatt cgataggtga accaacaggc
     2401 tcgacgtcag tgccgaccgg cgggccggcg gcgcccgcgg gacgcgagga ccagcccggc
     2461 caggctgacc accaccagca gcacggccgc gaggtgcagc cacacccagg ccgcggcgta
     2521 cagggccgcc atcgccaggc ccacccggac ggcgttgccc cggtacccgc gtccggggcg
     2581 gcgcgcgtcc cggacggcgc ggcgcacgat caggtacagc gctccggcgg cgccggcacc
     2641 aaccgcgatc ctcgtgccgg tcctcatcgg cgcatccctc cccgtcgggt cacctcttcc
     2701 tgccacgacc acggtagcca ccgggtacga cacgattccg tgtcgtaccc gccgggcgag
     2761 gctcgatccg gaggacggcg attacgtgcc tctgccccgc tgaccatcag tcccagatcc
     2821 gctcgaggtg ccagcggcct gcccggcggt cgtgatagag gtcgcacacc gcgccgccct
     2881 tcgcctcgac ccgccagtac tctcggtccc ggccgtcctc ccaccagcgg ccctcgatcc
     2941 gccagcggcc gatcacgttc gccacctcgt agcgccgtcc ccgccaggcg aacgccgccg
     3001 gcattccggc gtcgcgctcc accgtcacca gctcgtcgta gatcttcgac accccgcccc
     3061 acccgttcct cagcggaatc gaacgtatgt tcgagtggaa cggttaccgt acgcccagcg
     3121 ccgagccgct gtcaaggcca acagccactg gcccactcag tagcgggcgc ggcggccgac
     3181 gaagcccagg ccgatcagcg cgccgagcgc caccacccac agccccgcgg cggaggcgac
     3241 ggcgagaccg gagggcatcc gcgagatgcc gactcccgcg ccgaccagca gcgcgtgcac
     3301 caccgcgcag atcaccaggc actcgaccac cgagaacccg cgcccgagca gctggtcgta
     3361 gaggtgctcg cgatcgggtt cgagcagcgc cgagcggccg gccacccggc gcgccaccga
     3421 cagcagtagg tcgagcgcga acaggccgag gcacgtcccg gccccgacca tcagcgccgg
     3481 tccgccggcc agcaggagca ccgccaccgc gagcgtcgcc cccgccaggt acgaaccgct
     3541 gttgccgagg aacagccgcg ccggcgggcg gttgtggaac aggaacccga gggacgcccc
     3601 ggccaggagc agcggcagca gcgtcccccg gtcgtcgagc gcggcgacga ccgccagccc
     3661 gatccccgac agcgccgccg ccgagccggc cagcccgtcc atcccgtcca ccatgttcac
     3721 cgcgttgacg gcgccggcga agaacaccat cgccgcgagc cagccgagca ccgccccgag
     3781 cgggggcagg tccaggccgt tcgcggcgac gagcgcgccg agcgctacct gcgcgccgag
     3841 gcgaacgacc ggcgggaacc cgagcacgtc gtcggccagg ccgacgagcg cgatcccgaa
     3901 cagggcgacg gtcaggaacc agggcagcgg ccacgcgcgg gccgccagcc cggcggcgat
     3961 cccggcggcg acggccaggc cgccgaggta ggacaccggc tgcggatgcg gcttcaggcg
     4021 tccggtcggg aagtccaggg caccggtggc gagcccgagc caccgcgcgc cgaacccggc
     4081 cgccgccgcc acggccagag cgatggcggc cgcggcgagc ggggtcaccc gcgccggccc
     4141 cgatcgcgca ggagccgctc gtagacggcg agcgtgcgct cgatcacccg ctcctcgtcg
     4201 aagcgcaggg cgacccgccg ccggccggcg gcctccatct ccgtgcgcag gacgtcgtcc
     4261 tccacgagcc gcaggatcgc ccgggccagt gcgccgtgat cgcgcgccgg gaccagcagc
     4321 ccggtctcgc cgtcggccac cacctcccgg cagccccgga cgtccgtggc caccaccgcg
     4381 cgcgcactgg ccatcgcctc gatcgccgat cgggggacgc cctcccggta cgagggcagc
     4441 gcgaacaggt cgaacgcccg gtagacggcc ggcatgtccg cgccggccgc ctcgccggcg
     4501 aggaccacgc tctcccccgc ccgcacgcgc gccaggacct cgggcgacag gcgatccgtc
     4561 ttcgcggggt cctccatgcc caccgcgacg aacagcgcgt cggggcgcgc ccggcgcacc
     4621 acgtcggcgg cgcggaagaa ctccacgtac cccttctcgc ggaccagccg cccgaccatg
     4681 ccgaccacga accgcctgct cgtctcgggc ccggccgccc gttcccattt ggagcgcagc
     4741 gcgctgaccc gctccatcag gaccgcgtcg ggcccgaacc gggccgtgtc gaccccgttt
     4801 ccgagccatg ccgcccgccg cgccggcacc acgccgcggg ccaccgcgtc gcgatggtcc
     4861 tcggcgctct ggaacagctc gaagtcggag acgcgggacg agacccgatc cgccaggcgg
     4921 accaggctgc gcttcacggt cccgagatcg gccggcgcat acgagccgtg cacggtgttc
     4981 acgacgatcg ggaccccggc cagtcgcgcc gcgatccttc ccaggacgcc ggtcttcggg
     5041 ttgtgcgtgt ggacgatgtc gggccgctcg cggcggaaga gccgccgcag cgccaggagc
     5101 gcgcggaggt cctgcagcgg cgtccaggag cgcgtcagcg acgggatcgg gatgtggcgg
     5161 atgccgtgtt cctccagctc gcgaacgtgg gggcccggcg ccgagaccgc ggtcaccgcg
     5221 tagccctcct tgccgtagcg ctccagctgg ttcagcagga gcgcggcgag cgacagatcc
     5281 accgtggtca ggtgcagcaa cctcaccggg cgtcccgttc ccatcaccgc gtccatttca
     5341 tccgcgcgta cagccggtcc tcgagcgggg cgatgccgtc caggcgccgc cgcaggaaga
     5401 acgtaccgtc gctgcggcgg accggtgtgc gctggacccg gccgcggtcg accccgccgg
     5461 ggacgttctt cccgcggtcg ccgccgaccg cccaccggta gtgacgggtg acgagatcct
     5521 ggtggccgac gaccgcccgc gggtaggcga acaccaccgg ctcggtcccg agccgttcgg
     5581 cgatcacgcg cttcgactcg gccagctccc gctccgcccg tgccccgtcg atccggtcga
     5641 agtcgggatg ggtgaccgag tgcgacccga cctcgaccag gccggtctgc tgcatctggt
     5701 ggatggcatc ccacgccagc ggccggggcg gctcgccccg cccgcgcggg tgcgagagcg
     5761 gcgcgggggt ggtcccgtcg aggaagccgg tcgccaggaa cagcgtggcg gggatctcga
     5821 aggccgacag gaccggccag gcccgggcga agacgtcctg atacccgtcg tcgaacgtca
     5881 ccgcgaccag gtcgcggtcg accgcgctcg cctccacccg ctccagcccc gcgcgcagcg
     5941 ggaccaccgt gtagcgctcg cggagcagcc gcatctgctc ggtgaagcgg gcctcgggaa
     6001 ggtccatctc ctcccccatc cgcgcgccga cccggtggta gatcaggacg aagagcccgg
     6061 gttcacgctc gcgccgcagg taggcggccg gcaggagcgc gagctttacc gcgcgcttcg
     6121 cgacagagac gagcgcgaac cggacgccca cgccgggact ggtcggtcgg cctgctcctc
     6181 ctcaggcttc ggtccggggc ggagggactg cacaaccatc atgcagggga tcatcagcag
     6241 gaacggcagc acctgcgtcc gttgtcgcgc cagcaggccg aaattcgcca gcgatgtcag
     6301 gacgatcgag atgaacaccg tggagagcag caccatgatg gtcatcgcgt tccggcgcca
     6361 ggcgcgaagc accctgccga tcgcgcggaa cttgagcagg aacagcatca ggatgcccac
     6421 gccctccagg gattgcagcg ccggaagcag gccgcccgcc tcccacggga acgggcggaa
     6481 gttcgccgtg atcagcgcca gcgccaaccc ctgcggggtg aacgggctcg ccgggctgaa
     6541 gttcgagccc tcgtcggagt cgaagtcctg ctgctgggcc tgcgccacga cgctctggat
     6601 ggaggtgccc tccgtggcgc cgacgaactg gcgacccttg gcgatcagcg tcagggcccc
     6661 gagggccagg atcgggatca gcagcaggta ggcgagggct tggacgtgcg aggtccgcgc
     6721 gcgcgtcggg cgcagcgcga acgccgcgac gccggccacc gccagcgccg ccgcgagcgg
     6781 ggaccgcacc atgctgacca cgccgagccc gacgaccacg tacgccacgg ccttcgccga
     6841 cagcttccgg aacatcaggg cgaacccgta ggtggccacc ccgagcccca tcaacatggg
     6901 ggcgtccttc ccgagcgacg tggtccagta ccagaacgag gggaggaaga agatgaagat
     6961 ggcgtagagc cggcggtcgc cgtcggggaa cgacacccgg aaggccttgt agaagtgcca
     7021 cgacccgatg aaggccatcc cggcgaagac cacgaacccg gcgagcatcg acggggggat
     7081 cagcgtgtag aggaagccgg cggcgtagtt gatcgcgttc gtgtccgtgt acggcgggat
     7141 gagcgcggag tagttcccga cccggaagtc ctcggccagg ctgacgccgt tcgtgaagta
     7201 gccgcgggcg tcggagacgc ctccgtagaa catctgcgtg accaggaacc ggatcagcgc
     7261 tccgatgatg aagacggcca gtgccaggaa caggaccttc aggctgatcg tgcggtcctt
     7321 gcgccaggcg accggcagga tcagcgatcc ggccaccagg gtgaggccgg cgaacgccac
     7381 gtaggactgg atctcgagcg gcatcgagcc catcacccat gcggcgagta ccaccaggcc
     7441 ggcgatgagc gtccagacca tccgcgagag cgcgcggttg gacccggtga aggtggggga
     7501 ggcggtggcc atgcgcgccc gattctagaa gacctcgagg tcgcccaggg agagcgacca
     7561 ggaagcggcc cgcagcgggt cgggcccgct cccccttccc ccggcggcgc cgaccgggcg
     7621 gacgacgaag atcatcccct ggccggggag cggcgcgaac ccggcgcggg ccagggcgac
     7681 gcgcgcgggc cactcctggc cgaagtgggc cacggcgtgg tgcgcgccgc tcgcccgcac
     7741 ggcccgcgag agcccgcggc gcgcggccgc ggcgtccggg gccagcacct cgtcgaccac
     7801 ggcctccgcc agcggtccgc gctcccggac ccgcaggatc accagggcgt cgccgtccag
     7861 gagggcgtgg tacgggatcg gcccggcccc gtagcgccag cgcaggtagg ccggcgttcg
     7921 cggcgtcgcc agcgcgtgcc gcggacgaac ggcgcgggcc aggacccgtt ccgccacggc
     7981 cccctgcagc gcctcggcag cggggcgcag ggcgga
//
LOCUS       DASPWO010000045         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_939745, whole genome shotgun sequence.
ACCESSION   DASPWO010000045 DASPWO010000000
VERSION     DASPWO010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939745"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /gene="map"
                     /locus_tag="VEO00_01145"
     CDS             complement(<1..531)
                     /gene="map"
                     /locus_tag="VEO00_01145"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HYU56639.1"
                     /translation="MILYKSPSEIEKMRRAGRITAAAIERLVPEVRPGITTRDLDRVA
                     EAFIREKGATPSFLGYRGFTGSICTSINEQVVHGIPGSRVLKAGDLLSLDVGAIWEGY
                     HGDSAVTVFVGEPLSNEAEKLVRVTEESLEAGISQIRAGGRLSDIGHAVQQVVEGAGF
                     SVVREYVGHGIGRALHE"
     gene            complement(545..1204)
                     /locus_tag="VEO00_01150"
     CDS             complement(545..1204)
                     /locus_tag="VEO00_01150"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HYU56640.1"
                     /translation="MRVVLLGPPGAGKGTQAHRLADRFGLALISTGDIFRANLRDRTA
                     LGMEAKGYMDAGDLVPDDVVVRMVLARLSEPDAEPGFVLDGFPRTLGQAWSLEDALAE
                     AGGPLDAVLKFTIPDDVAVHRLLGRFTCPSCQRTYNVESNPPRTAGVCDVCGGRLMHR
                     DDDAEATVRHRIEVYHRDTEPLERLYDERGLLHPVEAVGDVDDVTERAVEAMKAGAAP
                     A"
     gene            complement(1215..2483)
                     /gene="secY"
                     /locus_tag="VEO00_01155"
     CDS             complement(1215..2483)
                     /gene="secY"
                     /locus_tag="VEO00_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HYU56641.1"
                     /translation="MLRAFVNAFKIPDLRKKILFTVFIIFIYRLGSHVPVPNVDVSAL
                     QGQLDASNQSVAQFLNLFSGGALTRFAVFALGIMPYITSSIIMQLLTVVIPKLEQWQK
                     QGEVGVKKITQWTRYLTVVLALLQSTGLVFLFNNQFNIIPAGKFTAPRIALIVMTLTA
                     GTAMIMWLGELITQRGIGNGMSILIFTSVISRLPAEGSAILEQGGPGKFGFILALAVA
                     IIFAVVFMEQGQRRIPVQYAKRVVGRRMTQGGSTYIPLKVNQAGVIPIIFASSVLYFP
                     ALLQNVVHWKPFQNFVNNVLVDSSHPVYMVFYGLLVVFFAYFYTAIAFNPVETADNIR
                     KYGGFVPGIRPGRPTAEYLDHILTRITLPGSLFLAVVALLPSVFLAIWAIQQFPFGGT
                     SILITVGVGLETMKQIESQLLMRHYEGFLR"
     gene            complement(2522..2956)
                     /gene="rplO"
                     /locus_tag="VEO00_01160"
     CDS             complement(2522..2956)
                     /gene="rplO"
                     /locus_tag="VEO00_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HYU56642.1"
                     /translation="MKVHHLRPAPGSKRPRKRVGRGIAAGQGKTAGRGTKGYLARHGS
                     KPGFEGGQMPLARRVPKWRGFTNPNREEWAVVNVERLQAFDAGATVTVEDLRSRGMVP
                     KKLPVKVLGRGDLDRKLTVRVHAFSASARAKIEGAGGSAEVL"
     gene            complement(2953..3138)
                     /gene="rpmD"
                     /locus_tag="VEO00_01165"
     CDS             complement(2953..3138)
                     /gene="rpmD"
                     /locus_tag="VEO00_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HYU56643.1"
                     /translation="MAKLRITQVRSVIDRPRPQKATIRALGLKRMHHTVVQEDRPEIR
                     GMVAKVRHLVTVEEVDE"
     gene            complement(3138..>3315)
                     /locus_tag="VEO00_01170"
     CDS             complement(3138..>3315)
                     /locus_tag="VEO00_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HYU56644.1"
                     /translation="PINIVHATVAGLRGLRTPEDVARLRGLDVNAVAPRPMLEAIAEA
                     EVRLDAARAEQGSA"
BASE COUNT          509 a         1180 c         1113 g          513 t
ORIGIN      
        1 ctcgtgcagg gcccggccga tcccgtggcc gacgtactcc cgaaccaccg agaaccccgc
       61 accctcgacg acctgctgca ccgcgtggcc gatgtcggag agccggccgc cggcgcggat
      121 ctgcgagatc cccgcctcga gcgattcctc cgtgacccgg accagcttct cggcctcgtt
      181 cgagaggggc tcgccgacga agaccgtgac cgccgagtcg ccgtggtagc cctcccagat
      241 cgcgccgacg tcgagcgaca gcaggtcgcc cgccttcagc acgcgcgagc ccgggatgcc
      301 gtggacgacc tgctcgttga tcgacgtgca gatcgagccg gtgaatcccc ggtagcccag
      361 gaaggagggg gtggcgccct tctcgcggat gaacgcctcc gcgacgcggt cgaggtccct
      421 cgtggtgatc ccgggacgca cctccggcac cagccgctcg atggcggctg ccgtgatccg
      481 cccggcacgg cgcatcttct cgatctcgga cggcgacttg tagaggatca cgctcgcgct
      541 ccgcctaggc cggtgcggct ccggccttca tggcctccac ggcgcgctcg gtgacgtcgt
      601 cgacgtcccc gaccgcctcc accgggtgca gcagtcctcg ctcgtcgtag agccgctcga
      661 gcggttccgt gtcgcgatgg tagacctcga tccggtggcg cacggtggcc tccgcgtcgt
      721 cgtcgcggtg catcagcctc ccgccacaca cgtcgcacac cccggccgtg cgcggcgggt
      781 tggactccac gttgtaggtc cgctggcacg aggggcaggt aaaccgccca agcagccggt
      841 ggacggccac gtcgtcgggg atggtgaact tcagcacggc gtcgagcggg ccgccggcct
      901 ccgccagcgc gtcctccagc gaccacgcct gccccagcgt tcgcgggaac ccgtccagga
      961 cgaatcccgg ctccgcgtcc ggctcgctca gccgcgcgag caccatccgc accaccacgt
     1021 cgtcggggac caggtctccg gcatccatgt accccttggc ctccatgccg agtgccgtgc
     1081 ggtcgcgcag gttcgcccgg aagatgtctc cggtcgagat cagggccaga ccgaatcgat
     1141 cggccagccg gtgtgcctgc gttcccttcc ccgcgccggg agggccgagc aggacgaccc
     1201 tcactccatc tccctcaacg caggaagccc tcgtagtggc gcatcagcag ctgggactcg
     1261 atctgcttca tggtctccag gccgacgccc accgtgatca ggatcgaggt gcccccgaag
     1321 gggaactgct ggatcgccca gatcgccagg aagaccgacg gcagcagcgc caccaccgcc
     1381 aggaacagcg agccgggcag cgtgatccgg gtcaggatgt ggtcgaggta ctcggccgtc
     1441 ggccgcccgg ggcggatgcc ggggacgaag cccccgtact tgcggatgtt gtccgcggtc
     1501 tccaccgggt tgaaggcgat cgccgtgtag aagtaggcga agaacaccac cagcagcccg
     1561 tagaacacca tgtagacggg gtgactcgag tccaccagca cgttgttcac gaagttctgg
     1621 aacggcttcc agtggacgac gttctgcagc agcgccggga agtacaggac cgacgaggcg
     1681 aagatgatcg ggatcacgcc ggcctggttg accttcagcg ggatgtaggt cgagccgccc
     1741 tgcgtcatcc gccggccgac cacccgcttg gcgtactgca ccgggatgcg gcgctggccc
     1801 tgctccatga acaccaccgc gaagatgatc gccacggcca gggcgaggat gaagccgaac
     1861 ttgccggggc cgccctgctc gaggatcgcg ctgccctcgg cgggcaggcg gctgatcacg
     1921 ctggtgaaga tgaggatcga catgccgttg ccgatgccgc gctgggtgat cagctcgccg
     1981 agccacatga tcatggcggt ccctgcggtg agcgtcatca cgatcagggc gatgcgcggc
     2041 gcggtgaact tgccggccgg gatgatgttg aactggttgt tgaacaggaa gaccagcccg
     2101 gtggactgca gcagggccag gacgacggtc aggtagcggg tccactgggt gatcttcttg
     2161 acccccacct cgccctgctt ctgccactgt tcgagcttgg ggatcacgac ggtcagcagc
     2221 tgcatgatga tcgagctcgt gatgtagggc atgatcccga gggcgaacac ggcgaaccgg
     2281 gtcaacgcgc cgccggagaa caggttcagg aactgcgcga cgctctggtt cgaggcgtcc
     2341 agctgcccct gcagcgcgga cacgtccacg ttcggcaccg gcacgtgcga gccgagccgg
     2401 tagatgaaga tgatgaagac cgtgaacagg atcttcttgc gaaggtccgg gatcttgaac
     2461 gcgttgacga acgccctgag catctcgtcc ttccctcggg cccgctagtc taccgaaccc
     2521 gctagaggac ctcggcgctg ccgccggccc cctcgatctt cgccctcgcc gatgccgaga
     2581 acgcgtggac ccggacggtc agcttccggt cgaggtcccc gcggccgagc accttcaccg
     2641 gcagcttctt cgggaccatg ccccgggagc ggaggtcctc gaccgtgacg gtggcgccgg
     2701 cgtcgaaggc ctgcaggcgc tcgacgttca ccacagccca ctcctcgcgg ttcgggttgg
     2761 tgaagccgcg ccacttcgga accctgcggg cgagcggcat ctgcccgccc tcgaagccgg
     2821 gcttcgagcc gtggcgggcc aggtagccct tcgtcccccg gccggcggtc ttgccctggc
     2881 cggccgcgat gccgcggccg acccgcttgc gcggacgctt cgacccggga gccgggcgca
     2941 ggtggtgcac cttcactcgt ccacctcctc caccgtgacc aggtggcgca ccttggcgac
     3001 catcccccgg atctcgggac ggtcctcctg caccacggtg tggtgcatgc gcttcagccc
     3061 gagcgcccgg atcgtcgcct tctgcggccg gggccggtcg atcacgctgc gcacctgcgt
     3121 gatccggagc ttcgccatca ggcgctcccc tgttccgcgc gggcagcgtc caggcgcacc
     3181 tcggcctcgg cgatcgcctc gagcatcggc cgcggcgcga ccgcgttcac gtcgaggccg
     3241 cgcagccggg ccacgtcctc gggcgtgcgc aggccgcgaa gcccggccac cgtggcgtgc
     3301 acgatgttga tcggg
//
LOCUS       DASPWO010000046         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_686789, whole genome shotgun sequence.
ACCESSION   DASPWO010000046 DASPWO010000000
VERSION     DASPWO010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686789"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VEO00_01175"
     CDS             complement(<1..210)
                     /locus_tag="VEO00_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HYU56645.1"
                     /translation="MLTALLIMLREGFEAALVVAIVYAYILRIGRRDLAGAMWQGVLG
                     AAALSVVMGVGIHLTVENLEGRARLL"
     gene            complement(313..>2074)
                     /locus_tag="VEO00_01180"
     CDS             complement(313..>2074)
                     /locus_tag="VEO00_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HYU56646.1"
                     /translation="MYTLDLGALVAGSRYRGDFEERLKKVLKEIRTRGDIVLFIDELH
                     TLVGAGAAEGAIDAASILKPMLARGELQTIGATTLEEYRKHLEKDAALERRFQPIQVQ
                     EPTVAHTIDILKGLRDRYEAHHRVTITDDALVASANLADRYISDRFLPDKAIDLIDEA
                     GSRMRIRRMTAPPDLREFDARIAEVRREKESAIDGQNFEKAASLRDSEKNLLNDKARR
                     EREWKSGDMDVVAEVDEEQIAEVLASWTGIPVFKLTEEETAKLLRMEDELHKRVVGQD
                     DAIRAVSQAIRRTRAGLKDPKRPSGSFIFLGPSGVGKTELSRTLAEFLFGDEDALIAL
                     DMSEYMEKHTVSRLIGSPPGYIGYEEGGQLTEAVRRKPFSVVLFDEIEKAHPDVFNTL
                     LQILEDGRLTDAQGRTVDFKNTVLIMTSNLGTRDIAKGTTLGFTARPDAKVDYERMRD
                     KVMDELKRHFRPEFLNRIDEVIVFHQLTRAQVKDIVDLLMRRVVEQLKSKDVAMELTD
                     AAKELLAEKGYDPALGARPLRRTIQRMVEDPLSEKLLWKEFNAGQLVVVDAEDGNIVF
                     RSLDQPADIDVPPAELAGKE"
BASE COUNT          286 a          699 c          728 g          361 t
ORIGIN      
        1 cagcaggcgg gcgcgccctt cgaggttctc cacggtcagg tggatcccga cgcccatgac
       61 caccgacagc gccgccgccc caagcacgcc ctgccacatg gcgccggcca ggtcgcgccg
      121 gccgatccgc aggatgtagg cgtagacgat ggcgacgacc agggccgcct cgaagccctc
      181 gcggagcatg atcagcagtg cggtcagcat cgttaggcaa gcctaacaga gcgcgcgcat
      241 gactgcgcaa cggcaaagcc cccggggatc ccgggggctt cgcctatccc tggggtggga
      301 caggtgggac gtctactcct tgccggcgag ctccgccgga gggacgtcga tgtcggccgg
      361 ctggtcgagc gagcggaaca cgatgttgcc gtcctcggcg tcgaccacca ccagctggcc
      421 ggcgttgaac tccttccaca ggagcttctc ggacagcggg tcctcgacca tccgctggat
      481 ggtgcggcgc agcgggcggg cgccgagcgc cgggtcgtag cccttctcgg cgagcagctc
      541 cttggccgcg tcggtcagct ccatggcgac gtccttggac ttgagctgct ccaccacccg
      601 ccgcatcagc aggtcgacga tgtccttcac ctgcgcccgc gtgagctggt ggaagacgat
      661 cacctcgtcg atccggttga ggaactccgg ccggaagtgc cgcttcagct cgtccatcac
      721 cttgtcgcgc atgcgctcgt agtcgacctt ggcgtcgggc cgggccgtga agccgagcgt
      781 ggtgcccttg gcgatgtcgc gggtgcccag gttggaggtc atgatcagca cggtgttctt
      841 gaagtcgacc gtgcggccct gggcgtcggt caggcgcccg tcctccagga tctgcagcag
      901 ggtgttgaag acgtccgggt gggccttctc gatctcgtcg aacagcacca cgctgaacgg
      961 cttgcgccgc accgcctcgg tgagctggcc gccctcctcg tagccgatgt agcccggcgg
     1021 ggagccgatc agccgcgaga cggtgtgctt ttccatgtac tcggacatgt cgagcgcgat
     1081 cagcgcgtcc tcgtccccga acaggaactc ggccagggtg cgggacagct cggtcttccc
     1141 gaccccggac gggccgagga agatgaacga gccggagggt cgcttggggt ccttgaggcc
     1201 ggccctggtg cgccggatgg cctgggagac cgcccggatg gcgtcgtcct ggccgacgac
     1261 ccgcttgtgc agctcgtcct ccatccgcag cagcttggcc gtctcctcct cggtgagctt
     1321 gaacaccggg atgccggtcc aggaggcgag cacctcggcg atctgctcct cgtcgacctc
     1381 ggcgaccacg tccatgtcgc cggacttcca ctcccgctcg cggcgcgcct tgtcgttcag
     1441 gaggttcttc tcgctgtcgc gcagcgaggc ggccttctcg aagttctggc cgtcgatcgc
     1501 cgattccttc tcgcgccgga cctcggcgat ccgcgcgtcg aactcgcgca ggtcgggcgg
     1561 ggccgtcatg cgacggatgc gcatacggct gccggcctcg tcgatcaggt cgatcgcctt
     1621 gtcgggcagg aagcggtcgg agatgtagcg gtcggccagg ttggcggagg ccaccagggc
     1681 gtcgtcggtg atggtgaccc ggtggtgggc ctcgtagcgg tcgcgcaggc ccttgaggat
     1741 gtcgatggtg tgggcgacgg tcggctcctg cacctggatc ggctggaagc ggcgctcgag
     1801 ggcggcgtcc ttctccaggt gcttgcggta ctcctcgagg gtggtggcgc cgatcgtctg
     1861 cagctcgccc cttgccagca tcggcttgag gatcgaggcg gcgtcgatcg cgccctcggc
     1921 cgcgccggcc cccaccaggg tgtgcagctc gtcgatgaac agcacgatgt cgccgcgggt
     1981 gcggatctcc ttgaggacct tcttgaggcg ctcctcgaag tcgccgcggt agcgcgagcc
     2041 ggccaccagc gcgcccaggt cgagggtgta catc
//
LOCUS       DASPWO010000047         6704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_347120, whole genome shotgun sequence.
ACCESSION   DASPWO010000047 DASPWO010000000
VERSION     DASPWO010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347120"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="VEO00_01185"
     CDS             <1..1606
                     /locus_tag="VEO00_01185"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HYU56647.1"
                     /translation="YDPQADGYFVHEKRTAGDVTAAREAGISFAAYRILLWRYAKAAS
                     LQETFDELVATMRSLCYRIDYTATEGGDPGALGNRIAAAVIDYGRRDGALEGRRYLDT
                     SYAPVNAPLVVAEDGTTMRDPNRWQPLALAQIVAQNGVPIPGNVQRAVGPHWGHVKGF
                     ALPPSAQGTPIDPGPPPHLDDPLTDSAFKRGAIDVIRDSSALDPSDPATIDIGPGALG
                     DNPLGTNDGDGRERNPVTGEPYAPNLVRRGDFSRALTEFWADGPQSETPPGHWNTIAN
                     EVSDAPGFAHRIGGQGPEVDRLEWDVKLYSALNGAVHDAAIAAWGLKGRYDSARPISM
                     IRYLGGKGQSSAPTGPSYDPDGLPIVPGLVEVITRASSRPGGRHEGMAGHVGEIAIRA
                     WRGPPADPADTSGVGWILATEWMPYQRPTFVTPAFPGFVSGHSTFSRAAAEVMTAFTG
                     SSYFPGGLSEWRIPAGSLTVEAGPSDDVTLQWATYYDAADQAGISRIFGGIHIPADDF
                     EGRRAGSQCGRDAWALASRYFEGTAL"
     gene            complement(2225..3211)
                     /locus_tag="VEO00_01190"
     CDS             complement(2225..3211)
                     /locus_tag="VEO00_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYU56648.1"
                     /translation="MSALRWGVLGAAKIAVEKVVPATMRADRCAVTAIASRDPDRART
                     VAGRLGIPTAYGSYEDLLGDPGVDAVYIPLPNHLHAEWTVAAARAGKHVLCEKPLAMN
                     AAEAERMVRSCADAGVLLMEAFMYRLHPSWEAVREIVGSGRIGPLRAVQSWFSYFNDD
                     PSNIRNIAAYGGGALMDIGCYCVNLSRMLFGAEPGRVEASVVRDPATGVDVLTSAILG
                     FDDGVATFTCSTRAEPDQRVHVYGTEGRLTLAIPFNIPPDRPAEVVVTAGGDPPVAPH
                     SEAVAFGPADPYAIQAERFAAAVLDGAPLPIPPEDAIGTMRAIDEIFRSGAR"
     gene            complement(3208..4275)
                     /locus_tag="VEO00_01195"
     CDS             complement(3208..4275)
                     /locus_tag="VEO00_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006605390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYU56649.1"
                     /translation="MARDDPLRVAVIGYGLGGAVFHAPLVASAPGMAVAAIVTGNPQR
                     QAAARAAFPDAAVHPTAEDLWRDAGALDLVVITTPNRFHVPLALAALEAGLPVVVDKP
                     LAATAAEARTLLDAARARGLPLTVFQNRRWDGDFLTVRRLIEEGALGQVIRFESRFER
                     WRPQLDPGAWRESAAPQDAGGLLYDLGSHLVDQAVVLFGRPTRVYAELERRREGAEVD
                     DDTFVALQQPGGVRSHLWMSATAASLGPRFRVLGMAGAYEKRGLDVQEEALAAGARPG
                     SPGWGREAPERWGTLSAGDDGRRVETLQGTYEEFYASVGRALREGTPPAVDPEDSIAG
                     LRVLEAAARSAAEGRVVELAW"
     gene            complement(4286..5323)
                     /locus_tag="VEO00_01200"
     CDS             complement(4286..5323)
                     /locus_tag="VEO00_01200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYU56650.1"
                     /translation="MRRQLALLEEACAAEGRDPASLARALLTGIGPERPLQSVEAFRD
                     LAGRCAELGITDLLLHCPQREGRFAGDLDVLEQIAAGGLGGWLALGSARLDHLRRANP
                     PRGKDGEVVTRLDGRVALVTGASRGIGAAVARALHGEGVRLGLASRSGDGLGIADAVA
                     RPCDVRRPADLEALVAEVVQRHGGLDIVVVNAGVGAYGPFLEMPADDVEEIVDVNVKG
                     ALNTLRAALPHVTRSDAGEVVMIASEAGRRGLPNEAVYCASKFAQVGLTRALDHELRP
                     LGVRCTNVCPGGVATDFAMGHGRTPDMPELAGMMSPEDVAQAVLFVLTRPRTHRILEM
                     AFRPVTETSWG"
     gene            5511..5933
                     /locus_tag="VEO00_01205"
     CDS             5511..5933
                     /locus_tag="VEO00_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015655223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56651.1"
                     /translation="MPSEERLSQSDTVVYIDRSDVLEGTFEELKAGIRELVGFVETHE
                     PQLIAYGFYVDEQAGQMTVVAVHPDSASLEFHIDVAGSGFSKLADLIKLRAIEVYGRL
                     SDRALELLQQKAAMLGDDGSVVVHERYAGFSRLPSATT"
     gene            5987..6433
                     /locus_tag="VEO00_01210"
     CDS             5987..6433
                     /locus_tag="VEO00_01210"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYU56652.1"
                     /translation="MTDADAATDPLTWDVVQRWLASSKYYWVVTSRPDGRPHTRAVWG
                     VWLEPRFHFTTSPETVMARNVVGNGYALMHPESAVDVVIVEGTVHRAAGTSLASVADA
                     YERKYGWRMDPEDPGMPFYSLEAQTALSWKARDPRGSAVRWRFDPA"
     gene            complement(6438..>6704)
                     /locus_tag="VEO00_01215"
     CDS             complement(6438..>6704)
                     /locus_tag="VEO00_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56653.1"
                     /translation="EAFARWADGLAATDPGEAAEAFRDAAGRFEEIGRPVERALILAD
                     PAAGEARAGRNGRAALEEARDILRRTGARLFLPDVEAALAAVTA"
BASE COUNT          991 a         2515 c         2299 g          899 t
ORIGIN      
        1 gtacgacccg caggccgacg ggtacttcgt ccacgagaag cggacggccg gcgacgtgac
       61 ggctgcccgg gaggccggga taagcttcgc cgcctaccgg atcctgctct ggcgctacgc
      121 gaaggccgcc agcctgcagg agaccttcga cgagctggtc gccacgatgc gctcgctctg
      181 ctaccgcatc gactacacgg cgaccgaggg cggcgacccc ggggcgctcg ggaaccgcat
      241 cgccgcggcc gtgatcgact acgggcggcg cgacggggcc ctggagggac ggcgctacct
      301 cgacacgagc tacgcgccgg tcaacgcgcc gctcgtggtc gccgaggacg ggaccacgat
      361 gcgcgacccg aaccgctggc agcccctcgc gctggcgcag atcgtggccc agaacggcgt
      421 accgatcccc ggcaacgtgc agcgcgccgt cggcccgcac tggggccacg tgaagggctt
      481 cgcgctccca ccatcggcgc agggcacgcc gatcgacccc ggcccgccgc cgcacctcga
      541 cgatcccctg accgactcgg cgttcaagcg gggggcgatc gacgtgatcc gggacagcag
      601 cgccctcgac ccgtccgacc cggcgacgat cgacatcggg cccggcgccc tcggcgacaa
      661 cccgctcggc acgaacgacg gcgacggtcg cgaacggaac ccggtgaccg gcgagccgta
      721 cgccccgaac ctcgtccgcc gcggcgactt cagccgcgcg ctgaccgagt tctgggccga
      781 cggaccgcag tcggagaccc cgccggggca ctggaacacg atcgccaacg aggtgtccga
      841 cgcgccgggg ttcgcgcatc ggatcggcgg ccagggcccg gaggtcgacc ggctggagtg
      901 ggacgtcaag ctgtactccg cgctgaacgg ggcggtgcac gacgcggcga tcgccgcctg
      961 ggggctgaag ggccgctacg actcggcgcg gccgatctcg atgatccggt acctgggcgg
     1021 caagggccag tcctccgctc cgaccggccc ttcgtacgat cccgacggcc tcccgatcgt
     1081 tcccggactg gtcgaggtga tcacccgcgc gtcgtcgcgg cccggcgggc ggcacgaggg
     1141 catggccggc cacgtcggcg agatcgcgat ccgcgcgtgg cgcggcccgc ccgccgatcc
     1201 ggccgacacg agcggcgtcg gatggatcct cgccacggag tggatgccgt accagcgccc
     1261 gacgttcgtc acgccggcct tccccggctt cgtctccggc cacagcacct tcagccgcgc
     1321 ggcggccgag gtgatgaccg cgttcaccgg cagctcctac ttcccgggcg ggctctccga
     1381 gtggcgcatc ccggcggggt cgctgaccgt cgaggccggg ccgagcgacg acgtcaccct
     1441 gcagtgggcg acctactacg acgcggcgga ccaggcgggg atctcacgca tcttcggggg
     1501 catccacatc cccgccgacg acttcgaggg acgccgggcc ggctcgcagt gcggcaggga
     1561 cgcctgggcg ctggcgagcc gctacttcga gggcaccgcc ctctgattcc actctggccc
     1621 gtcaactcct gtgtgcccgc cgcagtacga cctggccacc aacgccggca tggccatgct
     1681 gcataagaag aaggctccaa gtatcagctc ttgaccgaac cgacaccggc catgcttcgc
     1741 atgccgtcgg cccatccgat acggatggaa ggaggccctg gtctacttcg agagccccct
     1801 tgagttctgg tggctggatg aggattacca gggtccgtac acattgacca gtggccacta
     1861 tctcacttcc ccgacgagga tcccccgccg tggctgccgg aggataacaa tccgacctcc
     1921 ctgagacccc gtccccgaga cgacgacact cgatatccgg ctctgagcga atcgtcgagc
     1981 gatcgaggat cacgccctct cgggccgcgc aagaggctcg aggagttcgc acatcggcgt
     2041 cgggatccgt gagctggggt gcttcgtcag atgcgcgcca caccgtacag gcccgccgca
     2101 cggactccga ggcatcgttg cgctcgatca acgccccgtc cccagtgcgg cgtaccggca
     2161 ggagcaggtc ggctgggttc gtaccgacgg tcttgtgtcg cgcaaggttc aggagctcgt
     2221 tccgtcaacg ggctcccgac ctgaagatct cgtcgatcgc gcgcatggtg ccgatcgcgt
     2281 cctcgggtgg gatcgggagc ggcgcgccgt cgagcacggc ggcggcgaac cgctcggcct
     2341 ggatcgcgta gggatcggcg ggtccgaacg ccacggcctc cgagtgcggc gcgacggggg
     2401 ggtcgccgcc cgccgtcacg accacctcgg ccgggcggtc gggcgggatg ttgaacggga
     2461 tggccagggt cagtcgcccc tcggtcccgt agacgtggac ccgctggtca ggctcggcgc
     2521 gtgtcgagca cgtgaacgtg gcgacgccgt cgtcgaaccc caggatcgcg gacgtcagga
     2581 catccacgcc ggtggccggg tcacggacaa cgctagcctc cacccggccg ggctcggcgc
     2641 cgaacagcat ccgtgacagg ttgacgcagt agcagccgat gtccatcagc gcgccgccgc
     2701 cgtacgccgc gatgttgcgg atgttcgacg ggtcgtcgtt gaagtacgag aaccagctct
     2761 gcacggcccg cagcgggccg atccggccgg agccgacgat ctcgcgtacg gcttcccacg
     2821 acgggtgcag ccggtacatg aacgcctcca tcagcagcac gccggcgtcg gcgcaggacc
     2881 gcaccatccg ttcggcctcg gccgcgttca tcgccagcgg cttctcgcac aggacgtgct
     2941 tcccggcgcg ggcggccgcc acggtccact cggcgtggag gtggttcggc agcgggatgt
     3001 agacggcgtc gacgcccgga tcccccagca ggtcctcgta ggaaccgtac gccgtcggga
     3061 tgcccagccg gccggccacg gtccgagctc gatccggatc ccgcgacgcg atggccgtca
     3121 ccgcgcagcg atcggcgcgc atcgtggcgg ggacgacctt ctccaccgcg atcttcgccg
     3181 ccccgagcac cccccaccgc agcgcgctca ccacgccagc tccacgaccc tgccttccgc
     3241 ggcgctgcgc gcggccgcct ccagcacgcg cagcccggcg atcgagtcct ccgggtcgac
     3301 ggccggcggc gttccctccc gcagggcccg gccgacgctg gcatagaact cctcgtacgt
     3361 gccctgcagc gtctcgacgc gccgcccgtc atctccggcc gagagtgtcc cccaccgctc
     3421 gggcgcctcc cgcccccacc ccggcgaccc gggacgggcc ccggccgcca gggcctcctc
     3481 ctggacgtcg aggccgcgct tctcgtaggc cccggccatc cccaggacgc ggaaccgcgg
     3541 gccgagggag gccgcagtcg cgctcatcca gaggtggctc cgcaccccac cgggctgctg
     3601 cagggcgacg aaggtgtcgt cgtccacctc ggcgccctcc cgtcgccgct ccagctccgc
     3661 gtagacccgc gtcgggcggc cgaacagcac cacggcctgg tccaccagat ggctcccgag
     3721 gtcgtagagc agcccgccgg cgtcctgtgg ggcggcgctc tcgcgccatg ctccagggtc
     3781 gagctgcggg cgccagcgct cgaagcgcga ctcgaagcgg atcacctgcc cgagcgcgcc
     3841 ctcctcgatc agccggcgca ccgtcaggaa gtcgccgtcc caccgccggt tctggaagac
     3901 agtgagcggg aggccgcggg ctcgggcggc gtcgaggagt gtccgggcct ccgcggccgt
     3961 cgctgcgagc ggcttgtcca ccaccaccgg gaggccggcc tcgagggccg cgagcgccag
     4021 cgggacgtgg aagcggttcg gcgtggtgat caccaccagg tcgagcgcgc cggcgtcgcg
     4081 ccacaggtcc tccgcggtcg ggtggacggc ggcatcgggg aaggcggcgc gggccgccgc
     4141 ctgccgctgc gggttgccgg tgacgatcgc cgccaccgcc atccccggcg ccgaggcgac
     4201 cagcggcgcg tggaacaccg cgccgccgag gccgtagccg atcacggcca cacgaagagg
     4261 gtcgtcccgc gccatcctcg gctccctatc cccaggaggt ctccgtcacc ggacggaacg
     4321 ccatctccag gatccggtgg gtccgcggcc gggtcaggac gaacagcacc gcctgcgcca
     4381 cgtcctcggg cgacatcatg ccggcgagct ccggcatgtc cggcgtgcgc ccgtgcccca
     4441 tcgcgaagtc ggtggccacg ccgccgggac agacgttcgt gcagcgcacg ccgagcggcc
     4501 gcagctcgtg gtccagcgca cgggtcagcc cgacctgggc gaacttcgac gcgcagtaca
     4561 ccgcctcgtt cggcaggccg cggcggccgg cctcggaggc gatcatcacc acctccccgg
     4621 catcgctgcg cgtgacatgg ggcagcgccg cgcgcagcgt gttcagcgcg cccttcacgt
     4681 tgacgtcgac gatctcctca acgtcgtccg ccggcatctc caggaacggg ccgtacgcgc
     4741 ccaccccggc gttcaccacg acgatgtcca gcccgccgtg ccgctgcacg acctccgcca
     4801 ccagcgcctc caggtcggcc ggccggcgca cgtcgcaggg cctcgccacc gcgtccgcga
     4861 tccccaggcc gtcgccgctg cggctggcca gccccagacg caccccctcg ccgtgaagcg
     4921 cccgggccac cgcggccccg atcccccggc tcgcccccgt caccagcgcc accctcccat
     4981 ccagtctggt cacgacctca ccatcctttc cccttggggg gttcgctcgc cggaggtggt
     5041 cgagacgagc gctccccagg gcgagccatc ctcccaaacc accggccgcg atctgctcga
     5101 gcacgtccag gtcaccggcg aaccggccct cccgctgcgg gcagtgcagc aggaggtcgg
     5161 tgatcccgag ttcggcgcac cggccggcca ggtcccggaa tgcctccacc gactggagcg
     5221 gacgctccgg cccgatcccc gtcaggagcg cccgcgccag gctcgccggg tcgcgcccct
     5281 cggcggcgca cgcctcctcc aggagcgcca actggcgccg caccgcagcg agcgcaggcg
     5341 atgaccaccc cgacccgcac tcccagagcg taatgagtcc catcgccagc ggccgagtcg
     5401 tggaccttga ctcgtggacc agatgagacc cgccgcatga gaccttgact ccaccggacc
     5461 atgttgcgag ggtcgagcag gaaccgagct ggggatcggg gggatggggc atgccgagcg
     5521 aggagagact cagccagtcg gataccgtcg tctacatcga ccgctccgat gtcctcgagg
     5581 ggacgttcga ggaactcaag gccgggatcc gcgaactggt ggggttcgtc gagacgcatg
     5641 aaccgcagtt gatcgcgtac ggcttctacg tggatgagca agccggccag atgaccgtgg
     5701 tcgcggtcca ccctgattcg gcttcgctgg agttccacat cgacgtcgcc ggctccggat
     5761 tcagcaagct cgcggacctg atcaagctgc gagcgatcga ggtctacggc cggctcagcg
     5821 acagggcgct cgagctcctg caacagaagg cggcgatgtt gggagatgac ggcagcgttg
     5881 tcgtgcacga acgatacgcg gggttctccc gcctcccctc cgccacgacc tgagaccatg
     5941 cccacgccgt cgaggccgat cttccctcac cgcgccgagt acggtgatga ccgacgcaga
     6001 cgcggccacg gatccgttga cgtgggatgt ggtgcagcgc tggctggcat cctcgaagta
     6061 ctactgggtc gtcacgtccc gtccggatgg ccgcccccat acccgggccg tgtggggtgt
     6121 ctggctcgag cctcggtttc atttcacgac gagccccgag acggtcatgg cccgcaatgt
     6181 ggtcggaaac gggtacgccc tgatgcatcc ggagagcgcc gtggacgtgg tcatcgtcga
     6241 agggacggtc caccgggcag cgggcacgtc gctggccagc gtggccgacg cctacgaacg
     6301 gaagtacgga tggcggatgg accctgagga ccccggcatg ccgttctatt cactggaagc
     6361 tcagaccgcg ctcagctgga aggcccggga ccctcgcggt agcgcggtcc gctggcggtt
     6421 cgatccggcg taggcgctca tgcggtaacc gcggccagcg cggcctcgac atccgggagg
     6481 aacaatcgtg cgcccgtccg ccggaggatg tcccgtgcct cctccagggc ggcgcggccg
     6541 ttccggccgg cacgcgcctc gcccgcggcg gggtcggcca ggatcagggc gcgctccaca
     6601 gggcggccga tctcctcgaa ccgcccggcg gcgtcccgga aggcctcggc cgcctccccg
     6661 ggatcggtgg cggcgagccc gtcggcccac cgggcgaagg cctc
//
LOCUS       DASPWO010000048         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1110236, whole genome shotgun sequence.
ACCESSION   DASPWO010000048 DASPWO010000000
VERSION     DASPWO010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110236"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VEO00_01220"
     CDS             complement(<1..438)
                     /locus_tag="VEO00_01220"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HYU56654.1"
                     /translation="MLPSEDLRAGGAFERTRRPVDAQVRAEARAILSRVRAGGDAALL
                     ALTRELDGADLTHLRLPSEQLEQDAGAIPSGLAGAIERAAERLRALAAAQLPSPWQAE
                     SEGVRYGEVVTPLARVGCYVPGGRAAYPSTVLMTAVPAAVAGVE"
     gene            534..2057
                     /locus_tag="VEO00_01225"
     CDS             534..2057
                     /locus_tag="VEO00_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYU56655.1"
                     /translation="MKTLYRASRVRTQSWPEEGDWILVDDRHVERVGLGDPPEAHRVV
                     ELPGTTIIPGFVDAHVHLTGTGLVTAGLDLSGSRSAFDLVRLAREWVEGLDPSRRDRP
                     VVGQGWDESGWDVPGLPDAGTLDTIPAPALLIRADGHTSLANAALSEAAELGGLPGFG
                     RDDVGDRTGQLTAEANAAAQKWLFDGLTDAEVELAQLDACGLAAARGVTCVHELSIPD
                     KRGRRDFEVLMRQRERLPAHVVPYFGEVDIPYAMDFGLTRLGGDLFLDGSLGARTAAL
                     TDPYADGGGTGLLYHEGDELAELFHNAHLAGMQVGVHAIGDAAIEQAIGVWERVYRAL
                     DSRLRRHFRARRHRIEHFELPRGDQIERAAALGLAISVQPNFDAAWGAPGGMYERRLG
                     ETRAAGMNPLRSILERGLEVGAGSDSPVTPLDPMATLQAAEAHHDPAQRLSRAEAIRL
                     CTVGAARLAHLDDKKGRLEPGMQADFAAYDRDPFVEPSPEGMRPVLTVSQGRDVYAR"
     gene            2177..>2470
                     /locus_tag="VEO00_01230"
     CDS             2177..>2470
                     /locus_tag="VEO00_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56656.1"
                     /translation="MRKGAHWFTVLVAVGTLALMSLPTGAQAGSTQRYLVLYKSTQVP
                     ASAASDISAAGGTLVYSYDAIGVALAESSSASFADSMGASNKVEGVGATGGLGI"
BASE COUNT          329 a          883 c          935 g          323 t
ORIGIN      
        1 ctcgaccccg gcgacggccg ccggcacggc cgtcatcaac acggtcgagg ggtaggcggc
       61 ccgccccccg gggacgtagc agccgactcg cgccaggggg gtgacgacct cgccgtagcg
      121 cacgccttcg gactccgcct gccacggaga ggggagctgg gcggccgcca gcgcgcggag
      181 gcgttccgcc gcgcgctcga tcgcgccggc caggccggat gggatcgctc cggcgtcctg
      241 ctcgagctgc tcggagggga gccggaggtg ggtgaggtcg gctccgtcga gctcgcgggt
      301 cagcgccagg agcgcggcgt cgccgcccgc gcggacgcgc gacaggatcg cccgggcctc
      361 cgcccggacc tgcgcgtcaa ccgggcggcg tgttcgttcg aacgcgcccc cggccctgag
      421 gtcctcgctc gggagcatct gatcaagtcg atcgctgagc cgattgtagg ggggaaggaa
      481 cgggcggcgt ggccgcgggg acggcaccgg cgcggaggta tgctgcggcg gccgtgaaga
      541 ccctctaccg cgcgtcgcgc gtgcgcaccc agtcgtggcc ggaagagggg gactggatcc
      601 tcgtcgacga ccgccacgtt gagcgagtcg ggctcggcga tccgccggag gcgcaccggg
      661 tcgtcgagct gcccgggacc acgatcatcc ccggcttcgt cgacgcccat gtccacctga
      721 ccgggacggg cctggtgacc gcgggcctcg acctgtcggg ctcgcgctcg gcgttcgacc
      781 tggtgcgcct ggcacgggag tgggttgagg gcctcgaccc gtcgcggcgg gaccgcccgg
      841 tggtcgggca gggctgggac gagtccggct gggacgttcc cggccttccg gatgcgggca
      901 ccctcgacac gatcccggcg ccggcgctcc tgatccgcgc cgacggccac acgtccctgg
      961 cgaacgcggc actgtccgag gcggccgagc tgggggggct gcccggcttc ggccgcgacg
     1021 acgtcgggga ccggaccggg cagctcaccg ccgaggccaa cgccgcggcg cagaagtggc
     1081 tgttcgacgg gctgaccgac gccgaggtgg agctggcgca gctcgacgcc tgcggcctgg
     1141 ccgccgcgcg gggcgtgacc tgcgtccacg agctgtcgat ccccgacaag cgcgggcggc
     1201 gcgacttcga ggtgctgatg cgccagcgcg aacgactgcc tgcccacgtc gtcccgtact
     1261 tcggcgaggt ggacatcccg tacgccatgg acttcgggtt gacccggctg ggcggcgacc
     1321 tgttcctcga cgggtcgctc ggcgcgcgta ccgccgccct gaccgacccg tacgcggacg
     1381 gcggcggcac cggcctcctg taccacgagg gcgacgagct ggccgagctg ttccacaacg
     1441 cgcacctcgc cgggatgcag gtcggcgtcc acgccatcgg ggacgcggcc atcgaacagg
     1501 ccatcggggt ctgggagcgc gtctaccgcg cgctcgactc gcgactgcgc cggcacttcc
     1561 gtgctcggcg acaccggatc gagcacttcg agctgcctcg cggggaccag atcgagcgtg
     1621 cggcggcgtt ggggctcgcc atctcggtgc agccgaactt cgatgcggca tggggagcac
     1681 ccggggggat gtacgagcgc cggctggggg agacgcgcgc cgcggggatg aacccgctgc
     1741 gctcgatcct ggagcgcggc ctggaggtcg gcgccgggtc ggacagcccg gtcacgccgc
     1801 tcgacccgat ggccaccctg caggccgccg aggcacacca cgaccccgcg caacggctct
     1861 cgcgggcgga ggccatccgc ctctgcacgg tgggggccgc acgactggcc cacctggacg
     1921 acaagaaggg ccgcctggaa cccgggatgc aggcggactt cgccgcgtac gaccgcgacc
     1981 cgttcgtcga gccctcgccc gagggcatgc gcccggtcct gacggtcagc cagggccgcg
     2041 acgtctacgc ccggtagccg ggacgtccca ccctcccggc aggttccgtc ccctccgtgt
     2101 ccggttgaac ggcccatggc attcacacta gtctgcggct ggtctgtcaa tcagcgccct
     2161 gaagggggga gtgcggatgc gcaagggagc gcattggttc acggtgctcg tggcggtggg
     2221 gacgctcgcg ctgatgtccc tgccgaccgg cgcccaggcc ggcagcaccc agcgatacct
     2281 ggtgctgtac aagtcgacgc aggtaccggc cagcgccgcg agcgacatca gcgcagccgg
     2341 ggggaccctg gtctattcgt acgacgccat cggtgtggcg ctcgccgagt catccagcgc
     2401 ttcgttcgcc gacagcatgg gggcgagcaa caaggtcgag ggcgtcggcg ccaccggcgg
     2461 cctcgggatc
//
LOCUS       DASPWO010000049         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_262962, whole genome shotgun sequence.
ACCESSION   DASPWO010000049 DASPWO010000000
VERSION     DASPWO010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262962"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1797
                     /locus_tag="VEO00_01235"
     CDS             <1..1797
                     /locus_tag="VEO00_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HYU56657.1"
                     /translation="YLDATPEQRRRVHGRLAGVVADAPERARHRALAAEHPDAEVAAE
                     LDVVAGAVRSSAPSTAAELLELARALTPADLDEEAHGRALAAAAGHHEAGNTVKARQL
                     LEHTLDAVAVGPARAEALARLSRIRADTDDLGAALELGEAALRDPDTSPGLRVEVSAN
                     LAAAALLHGDVGRASAVAAAAVDDAEELGLDELLAELLGVLARGDVLTGRDGWPSLVD
                     LGVRLEEQGTGVDAGRSAGYARAQLQMWSGDLSGARQALRALRRYASDEGREQSLPLV
                     LCTLVQVECQAGNWSDAAGHAEEAHRASLLSAGEPQRALALSARALVEAHLGKADACR
                     ASAHEGLEAAVRAGAGAAAREIRATLGFLELSLGNHAGARRQLRPVLDETARLGITEP
                     GALRFVPDAVEATIAAGQPETAATLLAPFGERSRALGRPGGIGAATRGRALVAAARSD
                     FTAALSALAESGDALEGAGMPLELARTLLAQGRVGRRARQWSPARESLGRALAIFDSL
                     GARLWAEAARDELARVGGRAGSTGAELTVGERRVAELVGRGLKNREVADALFMSLKTV
                     EATLTRVYRKLGVHSRAELRSAVGDAGSPQVP"
     gene            complement(1785..>2572)
                     /locus_tag="VEO00_01240"
     CDS             complement(1785..>2572)
                     /locus_tag="VEO00_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HYU56658.1"
                     /translation="VTGEGDPTDGARRTELTEAATDYDMETARQLSETTEQLGQAQTE
                     LRFRKAELDDLLVRLEREKADFDAKVATAEKALWRAEVVGAARARGTTTIIGPSALAP
                     EEIAGWFRATGGRTTVAGISIEELAAIYVEEGAAAGVRGDIAFAQSIVETGSFNAIGP
                     NNFAGLGACDSCSDMTHFPTPRDGVRAQVQHLRNYADPNSRSDQLGNPPSPFWYGPGD
                     FDSFFAKGWCSTWEEMGAGNWATDPGYSAKVLAVYDRMLDYGT"
BASE COUNT          312 a          922 c         1003 g          335 t
ORIGIN      
        1 tacctcgacg ccacccccga gcagcgccgg cgggtccatg ggcggctggc cggggtcgtc
       61 gccgacgcgc ccgagcgagc ccggcaccgg gcgctggccg ccgagcaccc ggatgccgag
      121 gtcgccgccg agctcgacgt cgtcgccggc gcggttcgga gctcggcgcc gtcgaccgcg
      181 gcggagctcc tggagctggc acgcgcgctc accccggccg acctcgacga ggaggcccat
      241 ggccgcgccc tcgcggccgc ggccgggcac cacgaggccg gcaacacggt gaaggcgcgc
      301 cagctgctcg agcacacgct ggacgcggtc gccgtgggcc ccgcccgggc ggaggcgctc
      361 gcccggctga gccgcatccg tgccgacacc gacgatctcg gtgccgcgct cgagctcggg
      421 gaggcggcgt tgcgtgaccc ggacacctcg cccggtctac gggtcgaggt cagcgccaac
      481 ctggccgcgg ccgcgctgct ccacggggac gtcgggcggg cgagtgcggt cgcggccgcc
      541 gcggtcgacg acgccgaaga gcttggcctc gacgaactgc tggccgagct gttgggcgtg
      601 ctggcgcgcg gcgatgtgct caccggccgg gacggctggc cgtcgctggt cgacctcggc
      661 gtacgcctgg aagagcaggg gaccggcgtc gacgccggcc gcagcgccgg ctacgcccgg
      721 gcccagctcc agatgtggtc cggcgacctg tcgggagcgc gccaggcgct ccgcgccctg
      781 cgcaggtacg ccagcgacga gggacgtgag cagtcgcttc ccctggtgct gtgcaccctc
      841 gtgcaggtcg agtgccaggc gggcaactgg tcggacgcgg ccggccacgc cgaggaggcg
      901 catcgcgcca gcctgctgag cgcgggcgaa ccgcagcggg cgctggcgct gagcgcccgg
      961 gcgctcgtcg aggcccacct cggaaaggcg gacgcctgcc gcgccagcgc gcacgagggg
     1021 ctggaggcgg cggtgcgtgc gggcgcgggg gcggccgcac gcgagatccg ggcgacgttg
     1081 ggatttctgg agctctctct cgggaaccac gccggcgccc gccggcaact gcgcccggtg
     1141 ctcgacgaga ccgcccgcct cggtatcacc gagcccggcg cgctgcggtt cgtccctgac
     1201 gcggtcgagg cgacgatcgc cgccggccaa cccgagacgg ccgccacgct gctggcgccg
     1261 ttcggggagc ggagccgcgc gctgggacgt ccggggggca tcggcgcggc cacgcggggg
     1321 cgggcgctgg tggcggcggc acggagtgac ttcactgccg cgctcagcgc gctcgccgag
     1381 agcggggacg cgctcgaggg cgcgggcatg ccgctcgagc tggcccggac cctgctcgcg
     1441 cagggcaggg tcgggcggcg agcccggcag tggtcgccgg cgcgcgaatc gctcggacgc
     1501 gccctggcca tcttcgactc gctcggcgcc cgcctgtggg ccgaggccgc tcgggacgag
     1561 ctcgcccgcg tcggtggccg cgccggcagc accggagccg aactgacggt cggcgagcgg
     1621 cgcgtggccg agctggtcgg tcggggcctg aagaaccgcg aggtggccga tgccctcttc
     1681 atgagcctca agaccgtgga ggccaccctc acgcgcgtgt accgcaagct cggcgtgcac
     1741 tcgcgcgcgg aactgcgctc cgcggtcggc gacgcaggtt cgcctcaggt gccgtagtcg
     1801 agcatgcggt cgtagaccgc gagcaccttg gccgagtagc cggggtcggt ggcccagttc
     1861 ccggccccca tctcctccca ggtcgagcac cagcccttgg cgaagaagct gtcgaagtcc
     1921 ccggggccgt accagaacgg cgacggcggg ttgccgagtt ggtcggaacg cgagttggga
     1981 tcggcgtagt tacgaagatg ctggacctgg gcccgaacgc cgtcacgcgg ggtggggaag
     2041 tgggtcatgt cggagcacga gtcacaggcg ccgagtccgg cgaagttgtt cgggccgatt
     2101 gcgttgaacg atccggtctc gacgatggac tgggcgaacg cgatgtcgcc tcggaccccg
     2161 gcggccgcgc cctcttcgac gtagatcgcg gccagctcct cgatgctgat gccggcgacg
     2221 gtggtccgtc cgccggtggc gcggaaccag ccggcgatct cctcgggagc gagggcggac
     2281 ggaccgatga tcgtcgtcgt gcctctcgcc cgcgccgctc ctacgacctc ggcacgccac
     2341 aacgccttct ccgcggtcgc gaccttggcg tcgaagtccg ccttctcgcg ctcgagtcgg
     2401 acgaggaggt cgtcgagctc ggccttgcgg aaccgcagct cggtctgggc ctggcccagc
     2461 tgttcggtgg tctcgctgag ctgacgggcg gtctccatgt cgtagtcggt cgcggcctcg
     2521 gtgagctcgg tgcgccgggc gccgtcggtg gggtcgccct ccccggtgac ct
//
LOCUS       DASPWO010000050         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1110997, whole genome shotgun sequence.
ACCESSION   DASPWO010000050 DASPWO010000000
VERSION     DASPWO010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110997"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1807)
                     /locus_tag="VEO00_01245"
     CDS             complement(284..1807)
                     /locus_tag="VEO00_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYU56659.1"
                     /translation="MRLHRFVPFAAAALVASGCGGNSGKKTCFETGCGSGLVCETVVG
                     GAPACFDQIYVSGRVYDLAIADTSTNGLADARVVGQDVNGAPVSSVAISGAGGHYEFS
                     VAAPRNSDGSPASGSVTLRVDRAGFEPFPFGLRTALPIALTGATHGSGRWTVQGSLTD
                     VGLDAIASAPAGEITGTVQLPSSGGVLVVAECGGVGYTAVPGSDGTYAIFNVPDGTCE
                     VAAYAKGVNYTPVNVTVAGAGTNPVTADLARSAVQTATVSGSVQFVSSTFWDYTSVLL
                     VVDSTYDAARVRGIAPPGLKASGVTKGSNWTISGIPDGHYRVLAAFETDYVVRDPSDI
                     GGTAVLEFQVVNGVPLLMDGTTAAASLQGFKITGAVRLTAPIADGTGVCSTSSAPYAL
                     PADPGTLPVGGCTTTSATPSFAWMSYASTDVYEVTVIDELGATVWQAQVDKGASGVTY
                     GATASPVSNTLVDAQALATGATYQVRVKAQTTDPQSGAVDRTLSTSEDLLGVFTVVP"
     gene            complement(2283..>2396)
                     /locus_tag="VEO00_01250"
     CDS             complement(2283..>2396)
                     /locus_tag="VEO00_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYU56660.1"
                     /translation="EWLQVCRATVYRLVAEGQLPAVRVSSGAIRVRDSEGK"
BASE COUNT          368 a          806 c          892 g          330 t
ORIGIN      
        1 cgatcctccc ggcagcggcc ccgaacgctc gccgggttcg atgcggtcgg cttcggagcc
       61 ggcagaaatc gatcgcgtag cggttcggga gatcggacat ccgcctctcc gcagagctca
      121 gaaggcagcg gtcagggcca ccgatgaggg tgaccgggcc gctgagctgg ttgcggggcg
      181 tgcctcacgg cccgcccact acggcgccca tgaagaaacc cccggcaact cggagagtga
      241 ccgggggcta cgactgtcgg gagcgaggcg ggaggcgcct ggtctacggg acgacggtga
      301 agacgccgag gaggtcctcc gaggtcgaca gcgtccggtc cacggcgccg gactggggat
      361 cggtcgtctg cgccttgacg cggacctggt aggtggcgcc ggtcgcgagc gcctgggcgt
      421 cgaccagcgt gttcgagacg gggctcgcgg tcgcgccgta cgtcaccccg ctcgccccct
      481 tgtcgacctg cgcctgccag accgtcgcgc cgagctcgtc gatgaccgtc acctcgtaga
      541 cgtctgtgga ggcgtagctc atccacgcga acgagggggt cgcgctcgtg gtcgtgcatc
      601 cgcccacggg gagcgtgccg gggtcggccg gaagcgcata gggcgcggag gaggtggagc
      661 agacgcccgt gccatcggcg atcggcgcgg tgagccgcac ggctccggtg atcttgaagc
      721 cctggagcga cgccgccgcg gtggtgccgt ccatgaggag cggtacgccg ttcacgacct
      781 ggaactccag caccgccgtc ccgccgatgt cggagggatc gcggacgacg tagtcggtct
      841 cgaaggcggc gaggacgcgg tagtggccgt ccgggatccc cgagatggtc cagttgctgc
      901 ccttcgtgac gccgctcgcc ttgagcccag ggggcgcgat gccgcgcacc cgggccgcgt
      961 cgtaggtgga gtcgacgacg aggaggacgc tggtgtagtc ccagaacgtg gacgagacga
     1021 actggacgct gccgctcacc gttgcggtct gcaccgccga ccgcgcgagg tcggcggtga
     1081 ccgggttggt gccggcgcct gcgacggtca cgttgacggg ggtgtagttc acccccttcg
     1141 cgtaggcggc gacctcgcag gtgccatccg ggacgttgaa gatggcgtag gtaccgtcgc
     1201 tgccggggac cgccgtgtag ccgacgccgc cgcactccgc gacgacgagg acgccgccgg
     1261 aggacgggag ctgcacggtg ccagtgatct cgccggccgg ggcgctcgcg atggcgtcga
     1321 ggccgacgtc ggtgagggag ccctggaccg tccagcggcc ggagccgtgg gtcgcgcccg
     1381 tgagggcgat cggcagcgcg gtgcgcagcc cgaaggggaa gggctcgaag ccggcgcgat
     1441 ccacccggag cgtcacgctc cccgacgccg gcgagccgtc ggagttccga ggcgcggcga
     1501 cgctgaactc gtagtgcccg ccggcacccg agatggccac cgacgagacg ggggctccgt
     1561 tcacgtcctg cccgacgacg cgcgcgtcgg cgaggccgtt ggttgacgtg tcggcaatgg
     1621 cgaggtcata gacgcgcccg ctcacgtaga tctggtcgaa gcaggccggc gcgcccccga
     1681 cgacggtctc gcaaaccagg ccggagccgc agccggtctc gaagcaggtc ttcttccccg
     1741 agttgccgcc gcagccggag gcgacgagcg cggcagcggc gaaggggaca aaccgatgaa
     1801 gtcgcatgac gatcctttgg atgggagacg catcggccgg tggccggcga gtgccgggat
     1861 cggcgctggg aatgacatac catcaacgag gtacatcgat acggagcagt tccactcgcc
     1921 ggcggctcgc tctcccacgg gtgggcgccg cactcacctt cgtggcccgc ccgcgcgcca
     1981 cgcctacgcc ggctccgcgg gtgcgcgccc gccccgctgg accacgcgga ggctatcgcc
     2041 gatgagcagc cgaagtggtg cgagttgctc catgcacacg aggacgcggt gggaaccggc
     2101 ggcggctcgc cttccgatcg ctgagcttcc cgtgggtggc ggcgaaattc ggcccgtcgc
     2161 ggatggccga acgcgaccag cgttaccttc cggagcgcag ggtcgagacg aacgtgagca
     2221 ggtccgcttt cgaccagatc ccgcccgcga tgtgaacgtg cgactcgcca gcgtccaggc
     2281 cgctacttcc cttcggaatc gcgcacgcgg atggctccgg acgaaacacg cacggcagga
     2341 agctgcccct cggcaaccag tcggtacacg gtcgccctgc agacctgaag ccactc
//
LOCUS       DASPWO010000051         6587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_940506, whole genome shotgun sequence.
ACCESSION   DASPWO010000051 DASPWO010000000
VERSION     DASPWO010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940506"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VEO00_01255"
     CDS             <1..1039
                     /locus_tag="VEO00_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU56661.1"
                     /translation="SHLSLDVPAGEICVLVGPSGCGKTTTMKMINRLIEPTSGEIRVN
                     GTDVLHQDPVELRRGMGYVIQTIGLMPHRTVAQNIATVPHLVAWEEARIERRVDELLD
                     LFQLDREFRDRYPSELSGGQRQRVGVARALAADPPVMLMDEPFAAVDPIVRARLQDEF
                     LEIQRRLRKTIVFVTHDVDEAIKMADRIAILNLGGILEQHDTPEAILRAPANAFVERF
                     VGPERGLKRLALIPISEVGVEEGPVVAPGASPEEAVAAMDRFGLDWTSVVDGGELLGW
                     VDRDALAGASRAADGRPRRFSAYVTAKDSLRKALDSIVTSHTNVAVVVSEGQRYLGVL
                     TLERISREIVS"
     gene            1068..1772
                     /locus_tag="VEO00_01260"
     CDS             1068..1772
                     /locus_tag="VEO00_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU56662.1"
                     /translation="MIAQADEPLVRWDWVADHLTRFCRNCIPTVLAQHLQLTGIAVGV
                     GILIALPLAVLAFRHRRLYPPITWVTGVLYTIPSLALFMLLIPITHISTLTAEIGLVS
                     YTLLILIRNTVAGLDGVPEDTKEAALAMGFTRRQLLWRVELPLALPAIIAGIRIATVT
                     TVGLVTVTALIGMGGLGRLIRDGLQTFFPTPTVVGATLSVALAVAADVLLLGVQRGLS
                     PWTRRRLRRAGVRAEG"
     gene            1772..2539
                     /locus_tag="VEO00_01265"
     CDS             1772..2539
                     /locus_tag="VEO00_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU56663.1"
                     /translation="MGAFEGLVDWLSDPANWSGPEGVPARVGQHVQISGVSVAIATAI
                     ALPIGLVIGHTRRFEFLGVSIANLGRAIPSFALLSIAFQIVANTWTGLAFSIVPTVVA
                     MVLLGLPPILTNTYVGVQQVDRDTVEAARGMGMRGRQILGRLELPLAAPLIIAGVRTA
                     AVQIIATATLAAYVGQEGLGRYIRDGFAQSSEGQPMVLAGAILVALLAIVTDLLFGIV
                     ERVATPRTSAAGPRRTRRLRPDVENPETALTGTPANP"
     gene            2638..3582
                     /locus_tag="VEO00_01270"
     CDS             2638..3582
                     /locus_tag="VEO00_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYU56664.1"
                     /translation="MRYHRRHALGASLLLALALVAAACGGSSGGGGASPSGSSPAIER
                     GTIVVGVSGAFAENQLVAEMYAQVLEDAGYTVERQLDLDSRDTGNAALASGDIDVKPE
                     YLAFELPDLDPDADTAGTAENVFPRVQAAAEANDLTALEFSPANSTNVFVVTPDTANE
                     LGVATMSDLAPVAGDLVLGAPPDCDTNFPCEEGLKEVYGIEFKEIKKLDFGGPQTVAA
                     IDSGAVDVGELFSLDPTITAKGYVVLEDDKVLQPAGNFFALARTEILNGEVEDLLNGV
                     TTSLQTDEMVDLVGRVQVDHEDVADVAREYLESKGLLG"
     gene            complement(3586..4275)
                     /locus_tag="VEO00_01275"
     CDS             complement(3586..4275)
                     /locus_tag="VEO00_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HYU56665.1"
                     /translation="MDEPTGLLDLLDWKRRVFALYADVRAAGGSADAWERWRAGRDEL
                     FRSHPQSPLPPPERAGFPGLPYFAHDPGARVVGRLEPAEPERYEIASSVEGTYEFTRF
                     GTVRFALHGQDLALEAYWLAGYGGGLFLPFRDATAGTETYGAGRYLYDTVKGADLGTE
                     AGGLVLDFNFAYNPSCSYDPAWDCPLAPAPNWLDVPVRAGERFARPPDAPPPDTRGAR
                     PEGRALADAPV"
     gene            complement(4277..5980)
                     /locus_tag="VEO00_01280"
     CDS             complement(4277..5980)
                     /locus_tag="VEO00_01280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU56666.1"
                     /translation="MSSPATETAAMSPTTRRWTLVAVILGSGIVFLDTSVVNLALKAI
                     GEELPRSFVGRIEGQAYITGGYFVTLSALLILAGALNDFFGRRRVFALGLAGFLATSL
                     LCGLAPNTELLILFRILQGAAGAFVVPGSLAIITASFEGEAQGRAFGIWAGASAATSI
                     LGPFVGGVLVNSISWRAAFFVNVPFLLVALWATRAHVPESRDETASGHFDWLGSVVIV
                     LAVGGLAFGTIRGQQDQWQSSAAFISLAIGGLAAIAFPILMVKRSNPLAPPSLFRSRN
                     FTVTNLSTFVIYGALYVFLTFQGLFLIGTLGYNEPAAGIAGLPATLLLALFSTRFGKL
                     AARYGPRLFMTAGPAIMAVGTLWLVRIPAGSAAWVFGTGPGQRILPPGDYFLDLLPAF
                     LVFGAGLMVMVAPLTTALMNSVPRRNSGVASAINNAISRVGSPLVTAVIFVAIVSSFY
                     SSIQRQVPGVDTSSSDFRNEVAALNPPPEDADPRLANAIRTASTDAFHLAVVVGAALL
                     LLGAAINAAGIRNPDPRTPPWADASVRAEGEAEPLSAPAPAPSHAKRGARERHEHPPP
                     H"
     gene            complement(6009..6383)
                     /locus_tag="VEO00_01285"
     CDS             complement(6009..6383)
                     /locus_tag="VEO00_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYU56667.1"
                     /translation="MSARTDTAARLFELIGARKFEEAKALLTPDFVQEWPQSGERIRG
                     PDNAMAVNENYPGLPTFALRRIVEGGDLVVAETTLSYGGTIVNGVHVFEFQGDRVARE
                     TDYFSEPFHAPDWRARWVERME"
     gene            complement(6396..>6587)
                     /locus_tag="VEO00_01290"
     CDS             complement(6396..>6587)
                     /locus_tag="VEO00_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYU56668.1"
                     /translation="RILGRDDLWIAEMDLDYGSGAPYKGVFILEFDADGKVVRETDYF
                     AEPFEPAEWRTAWVSVDRD"
BASE COUNT          966 a         2350 c         2374 g          897 t
ORIGIN      
        1 cagccacctc tcactcgacg tccccgccgg ggagatctgc gtcctggtcg ggccgtccgg
       61 gtgcggcaag accaccacga tgaagatgat caaccggctg atcgagccga ccagcggcga
      121 gatccgggtg aacggcactg acgtcctgca ccaggacccc gtcgagctgc gccgggggat
      181 gggctacgtg atccagacga tcggcctgat gccccaccgc accgtggcgc agaacatcgc
      241 gacggttccg cacctcgttg cctgggagga ggcccggatc gaacgccgcg tcgacgagct
      301 gctcgacctg ttccagctgg accgggagtt ccgagaccgc tacccgagcg agctgtccgg
      361 cgggcagcgc cagcgcgtcg gcgtggcgcg ggcgctcgcc gccgacccgc cggtgatgct
      421 gatggacgag ccgttcgcgg cggtcgaccc gatcgtccgc gcgcgcctgc aggacgagtt
      481 cctcgagatc cagcgacgcc tgcgcaagac gatcgtgttc gtcacgcacg acgtcgacga
      541 ggcgatcaag atggccgacc gcatcgcgat cctgaacctc ggcggcatcc tcgagcagca
      601 cgacaccccg gaggcgatcc tgcgcgcgcc ggcgaacgcg ttcgtcgagc gcttcgtcgg
      661 ccccgagcgc gggttgaagc ggctggcact gatcccgatc tcggaggtcg gcgtggagga
      721 gggccccgtc gtggcccccg gcgcgagccc ggaggaggcc gtcgcggcga tggatcgctt
      781 cggtctggac tggaccagcg tggtcgacgg cggcgagctc ctcggctggg tcgaccgcga
      841 cgccctggcc ggggcgtcgc gggcggcgga cggccggccg cggaggttca gcgcctacgt
      901 cacggcgaag gactcgctgc gcaaggcgct cgattccatc gtcacctccc acaccaacgt
      961 cgccgtcgtg gtcagcgagg gacagcgcta tctcggcgtc ctgacgctcg agcgcatctc
     1021 gcgggagatc gtgtcgtgag cgggggcttc taccccccca aggaagcatg atcgcgcagg
     1081 ccgacgagcc cctggtccgc tgggactggg tggccgatca cctgacgcgc ttctgccgca
     1141 actgcatccc gaccgtgctg gcccagcacc tccagctcac ggggatcgcc gtcggcgtgg
     1201 gcatcctgat cgccctgccg ctggcggtcc tcgccttccg gcatcgccgc ctgtacccgc
     1261 cgatcacctg ggtgaccggc gtgctctaca cgatccccag cctggccctg ttcatgctgc
     1321 tcatcccgat cacccacatc tccacgttga ccgcggagat cggcctggtg agctacacgt
     1381 tgctgatcct gatccggaac acggtggccg gcctggacgg cgtccccgag gacacgaagg
     1441 aggcggcgct ggcgatgggc ttcacgcgcc ggcagctgtt gtggagggtg gagctgccgc
     1501 tggccctccc ggcgatcatc gccgggatcc ggatcgccac cgtgacgacg gtcgggctgg
     1561 tcacggtgac cgcgctgatc gggatgggcg gtctcgggcg gctcatccgc gacggcctgc
     1621 agacgttctt cccgaccccc acggtggtcg gggcgaccct ctcggtcgcg ctcgcggtcg
     1681 ccgccgacgt cctgcttctc ggcgtgcagc gcgggctgtc cccgtggacg cgccgcaggc
     1741 tgcgacgcgc gggtgttcgg gcggagggtt gatgggcgcg ttcgagggcc tggtcgactg
     1801 gctgagcgat ccggcgaact ggtcgggccc ggagggggtc cccgcccgcg tcgggcagca
     1861 cgtgcagatc tcgggcgtgt cggtggcgat cgccacggcc atcgctctgc cgatcggcct
     1921 ggtcatcggg cacacgcgcc ggttcgagtt cctcggcgtg agcatcgcga acctgggccg
     1981 ggcgatcccg tcgttcgcgc tcctgtcgat cgccttccag atcgtggcca acacgtggac
     2041 cgggctcgcg ttcagcatcg tcccgaccgt cgtcgccatg gtcctgctcg ggctgccgcc
     2101 gatcctgacc aacacgtacg tcggcgtgca gcaggtcgac cgcgacaccg tggaggccgc
     2161 ccgcgggatg gggatgcgcg ggcggcagat cctggggcgc ctggagctgc cgctcgccgc
     2221 gccgctgatc atcgccggcg tccgcacggc ggcggtgcag atcatcgcca cggccaccct
     2281 ggccgcgtac gtcggccagg agggtctcgg gcgctacatc cgcgacggct tcgcgcagtc
     2341 ctcggagggg cagccgatgg tgctggccgg ggcgatcctc gtcgccctgc tggcgatcgt
     2401 gaccgacctg ctgttcggca tcgtggagcg cgtcgccacg ccccgcacgt ccgcggccgg
     2461 accccgccgt acccgccgcc tgcggccgga cgtggagaac ccggagacgg ccctgaccgg
     2521 cacccctgcg aacccctgag gccaccatca cgggctcgct ttgcgagggc gctgataggg
     2581 tcggtgtgcc cggattccag ggggtgggca cccccacgac aggatgggag gagacccatg
     2641 cggtatcacc gcaggcacgc cctcggggcg agcctcctcc tcgcgctcgc gctcgttgcc
     2701 gcggcgtgcg gggggagctc gggtgggggc ggcgcgagcc cgagcgggag ctcgccggcc
     2761 atcgagcgcg gcacgatcgt cgtcggcgtc tcgggagcct tcgccgagaa ccagctcgtg
     2821 gccgagatgt acgcgcaggt gctggaggat gccggctaca cggtggagcg ccagctcgac
     2881 ctggacagcc gcgacaccgg caacgccgcc ctcgcgagcg gcgacatcga cgtgaagccc
     2941 gagtacctgg cgttcgagct cccggatctc gacccggacg cggacaccgc cggcaccgcc
     3001 gagaacgtct tcccgcgagt gcaggccgcc gcggaggcca acgacctgac cgcgctcgag
     3061 ttctccccgg cgaacagcac gaacgtcttc gtcgtcaccc cggacacggc gaacgagctg
     3121 ggggtcgcca cgatgagcga cctggcgccg gtcgccgggg acctcgtgct cggggcgccg
     3181 ccggactgcg acacgaactt cccctgcgag gagggcctga aggaggtcta cgggatcgag
     3241 ttcaaggaga tcaagaagct cgacttcggc ggcccgcaga ccgtcgccgc gatcgacagc
     3301 ggcgcggtgg acgtcggcga gctgttctcg ctcgacccga cgatcaccgc caagggctac
     3361 gtcgtcctgg aggacgacaa ggtcctgcag ccggccggca acttcttcgc cctggcccgg
     3421 accgagatcc tgaacggcga ggtcgaggac ctgctgaacg gcgtcacgac gtcgctgcag
     3481 accgacgaga tggtcgacct ggtcggccgc gttcaggtcg accacgagga cgtggccgac
     3541 gtcgcacggg agtacctgga gagcaagggg ctgcttgggt agcggtcaga ccggagcgtc
     3601 cgcgagggcc cgtccttcgg ggcgggcccc tcgcgtgtcc gggggcggcg cgtcgggcgg
     3661 gcgagcgaag cgctcgccgg cgcgcaccgg gacgtccagc cagttcggcg cgggggccag
     3721 cgggcagtcc cacgccgggt cgtacgagca ggacgggttg taggcgaagt tgaagtcgag
     3781 caccaggccg ccggcctcgg tcccgaggtc ggcccccttc acggtgtcgt acaggtagcg
     3841 gccggcgccg taggtctcgg tgccggcggt ggcgtcgcgg aacggcagga agagcccgcc
     3901 gccgtagccc gccagccagt aggcctcgag cgccaggtcc tggccgtgca gcgcgaagcg
     3961 caccgtgccg aaccgggtga actcgtacgt gccctcgacg ctcgatgcga tctcgtaccg
     4021 ctcgggctcg gccggctcga ggcggcccac cactcgcgcg cccgggtcgt gggcgaagta
     4081 cggcaggccg gggaaccccg cccgctcggg cggcggcagc ggggactgcg ggtgcgagcg
     4141 gaacagctcg tcgcgccccg cccgccagcg ctcccaggcg tccgccgagc cgccggccgc
     4201 ccggacgtcc gcgtacagcg cgaacacgcg ccgcttccag tcgagcaggt cgagcaggcc
     4261 cgtcggctcg tccacgtcag tgtggcggtg ggtgctcgtg ccgctcgcgc gcgccgcgtt
     4321 tcgcatggga cggcgccggc gccggcgccg atagcggctc cgcctcgccc tcggcgcgca
     4381 ccgaggcgtc ggcccacggc ggggtgcgtg ggtcggggtt gcggatgccg gccgcgttga
     4441 tcgccgcccc gagcagcagc agcgccgcgc cgaccaccac cgccaggtgg aaggcgtcgg
     4501 tcgaggccgt gcggatcgcg ttcgccaggc gggggtcggc gtcctccggg ggcgggttca
     4561 gcgcggcgac ctcgttgcgg aagtcggacg acgatgtgtc cacgccgggg acctggcgct
     4621 ggatcgagct gtagaagctc gacacgatgg ccacgaagat gacggcggtg acgagcggcg
     4681 acccgacgcg cgagatcgcg ttgttgatcg ccgaggccac gcccgagttg cggcggggaa
     4741 ccgagttcat cagcgccgtc gtcagcggcg cgaccatcac catcagtccg gccccgaaga
     4801 cgaggaacgc cggcagcagg tcgaggaagt agtcgccggg cgggaggatc cgctgccccg
     4861 gcccggtccc gaacacccat gccgcgctcc cggccgggat acgcaccagc cacagcgttc
     4921 cgacggccat gatcgccggg ccggccgtca tgaacaggcg cggcccgtag cgcgcggcga
     4981 gcttcccgaa gcgcgtcgag aacagcgcca gcagcagggt ggcggggagc ccggcgatcc
     5041 cggcggcagg ttcgttgtag ccgagcgtgc cgatcaggaa cagtccctgg aacgtcagga
     5101 agacgtacag cgcgccgtag atcacgaacg tcgagaggtt cgtgacggtg aagttccgcg
     5161 agcggaacag ggagggcggc gccagcgggt tcgaccgttt caccatcagg atcgggaacg
     5221 cgatcgccgc gagaccgccg atcgccaggc tgatgaacgc ggccgaggac tgccactggt
     5281 cctgctgtcc gcggatcgtc ccgaacgcca gtccgcccac ggcgagcacg atcaccaccg
     5341 accccagcca gtcgaagtgg ccgctggcgg tctcgtcgcg cgactcgggc acgtgcgcgc
     5401 gcgtggccca cagcgccacg agcaggaacg ggacgttgac gaagaacgcg gcgcgccagg
     5461 agatcgagtt caccagcacg ccgccgacga acgggccgag gatgctcgtc gccgcggagg
     5521 ccccggccca gatcccgaac gcgcgtccct gcgcctcgcc ctcgaagctc gcggtgatga
     5581 tcgccagcga ccctgggacg acgaacgctc cggccgcgcc ctgcaggatc cggaacagga
     5641 tcagcaactc ggtgttgggc gccagcccgc agagcaggga ggtggccagg aacccggcca
     5701 gtcccagcgc gaacacgcgg cggcgcccga agaagtcgtt cagagcgccg gccaggatca
     5761 gcagggccga gagcgtcacg aagtagccgc cggtgatgta ggcctgcccc tcgatccgcc
     5821 ccacgaacga gcgggggagc tcctcaccga tcgccttcag cgcgaggttc accaccgagg
     5881 tgtcgaggaa gacgatcccc gagccgagga tcaccgcgac gagcgtccag cgccgcgtgg
     5941 tcgggctcat cgcggcggtc tccgtcgccg gactgctcat gcccgcgcca acgtgggcgg
     6001 caccgcccct attccatccg ctcgacccag cgcgcccgcc agtccggggc gtggaagggc
     6061 tccgagaagt agtcggtctc gcgcgcgacg cggtcgccct ggaactcgaa gacgtggacg
     6121 ccgttgacga tcgtcccgcc gtagctgagc gtcgtctcgg ccaccaccag gtcgccgccc
     6181 tcgacgatcc gtcgcagggc gaacgtcggc aggcccgggt agttctcgtt gaccgccatc
     6241 gcgttgtcgg gcccacggat gcgctcgccg gactgcggcc actcctggac gaagtccggc
     6301 gtcaggagcg ccttcgcctc ctcgaacttg cgcgccccga tcagctcgaa gagccgggcc
     6361 gccgtgtcgg tgcgtgcgct caccgatccc ctccctcagt cgcgatccac gctcacccac
     6421 gccgtgcgcc actccgccgg ctcgaagggc tccgcgaagt aatcggtctc ccgcaccacc
     6481 ttgccgtccg cgtcgaactc gaggatgaac acacccttgt acggcgcgcc gctgccgtag
     6541 tcgaggtcca tctccgcgat ccacaggtcg tcccggccga ggatccg
//
LOCUS       DASPWO010000052         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_687740, whole genome shotgun sequence.
ACCESSION   DASPWO010000052 DASPWO010000000
VERSION     DASPWO010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687740"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VEO00_01295"
     CDS             complement(<1..587)
                     /locus_tag="VEO00_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56669.1"
                     /translation="MARVTVFGAGAAGTALAMHLARAENDTVLWASDFDARVLPFLDG
                     ERKHPNLPAYLPESLRVMGPQELADAAKGAEVAVMGANSGGARSLAAIVVDQLEDDLT
                     VLSLAKGLEPDSRKRMSEVYREVIGHGRVVSMGGPCLAQEVAEELPSAVVFACGLPEI
                     AEGLRPVFETRAFRVTVTDDVLGLEYCTVLKNVAA"
     gene            632..1531
                     /locus_tag="VEO00_01300"
     CDS             632..1531
                     /locus_tag="VEO00_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYU56670.1"
                     /translation="MSARNLGLLVLGVAAVSVSSILIRLAEDAPPLVIAFYRNAFAAA
                     LLLPFALTRRRAELRALTRRQWGACAASGAFLAAHFALWIPSLSFTTVAASTVLVTTQ
                     PIWVGAAGHWWLGERLTRAAVVGIAVALAGAVVVSGSDFGLSDRGLLGDLLALGGAWA
                     AAGYFLIGRRVRQGLSLVTYAAIVYSTCAALLAVVVAVSGQPFLGHAPNVWLLFLLMA
                     LVPQILGHTVFNFLLGDVEAAVIAIAVMGEPVGATVLALVILGESPPLATVLGGALIL
                     AGVYLAIAAQARRRAAPIPVPVE"
     gene            1567..1878
                     /locus_tag="VEO00_01305"
     CDS             1567..1878
                     /locus_tag="VEO00_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYU56671.1"
                     /translation="MDEETRGELIERYRRGFTLVEEGDDENAFAARLHYADRPIEAAL
                     AAFRAARETTSQLFETMTGDDWAREGTHTESGRYTAEDWLRATAAHAYDHGAQIRAAL
                     G"
     gene            1889..>2313
                     /locus_tag="VEO00_01310"
     CDS             1889..>2313
                     /locus_tag="VEO00_01310"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HYU56672.1"
                     /translation="MAKPAATVDVRDAQVIKHDRAFFLSDEFGDVPADNRAALGLYYR
                     DTRFLSSLELAVNDLRPILLHSSTERNYSQIVEMAFPQVVADPAGFEVKENVGVSRQR
                     VLSDSLLERIRISNFGRRPQSVRLTMDFAADFLDIFEVRG"
BASE COUNT          289 a          869 c          808 g          347 t
ORIGIN      
        1 atcgcggcca cgttcttcag cacggtgcag tactccaggc ccagcacgtc gtcggtcacc
       61 gtgacccgga acgcacgggt ctcgaacacc ggccgaaggc cttcggcgat ctccggcagc
      121 ccgcacgcga acaccaccgc cgagggcagc tcctcggcga cctcctgcgc gaggcacggc
      181 ccgcccatcg acaccacccg cccgtgcccg atcacctcgc gatacacctc cgacatccgc
      241 ttgcggctgt cgggctccag gcccttggcc aggctgagca cggtcaggtc gtcctccagc
      301 tggtcgacga cgatggcggc cagcgaccgc gcgccgccgg agttggcccc catcaccgcg
      361 acctccgcgc ccttcgccgc gtcggcgagc tcctggggcc ccatcacccg caggctctcg
      421 gggagatatg cgggcaggtt ggggtgcttg cgctcgccgt ccaggaacgg caggacgcgg
      481 gcgtcgaagt cgctcgccca gagcacggtg tcgttctcgg cccgagcgag gtgcatcgcc
      541 agggccgtgc cggccgcccc ggccccgaac acggtgaccc gcgccatgtc cgcaatctag
      601 ccgaacggcg ggctcccgga taatgggccg cgtgtcggcg cggaacctcg gcctcctggt
      661 gctcggcgtg gccgcggtgt cggtctcctc gatcctgatc cgtctggccg aggacgcgcc
      721 accgctcgtc atcgccttct atcgcaacgc gttcgccgcg gcgctcctgc tgccgttcgc
      781 gctcacccgg cgccgcgccg agctccgtgc gctcacccgg cgccagtggg gggcgtgcgc
      841 ggcctccggc gccttcctgg cggcccactt cgcgctgtgg atcccgtcgc tgtccttcac
      901 cacggtcgcg gccagcacgg tcctggtcac cacccagccg atctgggtcg gggcggccgg
      961 ccactggtgg ctgggggagc ggctgacgcg ggccgccgtg gtcggcatcg ccgtggccct
     1021 ggccggggcc gtggtcgtct cgggcagcga cttcgggctg tcggaccgcg ggctgctcgg
     1081 cgacctcctg gcgctcggcg gcgcctgggc cgcagccggg tacttcctga tcgggcggcg
     1141 ggtacgccag ggcctctcgc tcgtcaccta cgccgcgatc gtctactcga cctgcgcggc
     1201 gctcctcgcg gtcgtggtgg ccgtctccgg ccagccgttc ctcggacacg cgccgaacgt
     1261 gtggctgctg ttcctgctga tggcgctcgt gccgcagatc ctcgggcaca cggtcttcaa
     1321 cttcttgctc ggcgacgtgg aagccgcggt gatcgccatc gcggtgatgg gcgaaccggt
     1381 cggcgccacg gtgctggccc tggtgatcct cggcgagtcc ccgccgctcg ccaccgtcct
     1441 cggcggcgcg ctgatcctcg ccggggtgta cctggcgatc gccgcgcagg cccgccgccg
     1501 cgcggcgccc atcccggtcc cggtcgagta gcctccgcct ggacggccgg cgcggaggag
     1561 gagcccgtgg acgaggagac gagaggggag ctgatcgagc ggtaccggcg ggggttcacc
     1621 ctcgtcgagg agggggacga cgagaacgcc ttcgcggcac ggctgcacta cgcggaccgg
     1681 ccgatcgagg cggcgctcgc cgcgttccgc gcggcccggg agaccacgtc gcagctcttc
     1741 gagacgatga ccggggacga ctgggctcgc gaggggacgc acacggagag cggacgctac
     1801 acggccgagg actggctacg cgccaccgct gcgcacgcct acgatcacgg cgcccagatc
     1861 cgcgcggcgc tcggctgacg agggtcccgt ggcgaaaccc gcggccaccg tcgacgtccg
     1921 cgacgcccag gtcatcaagc acgaccgggc attcttcctg tccgacgagt tcggcgacgt
     1981 cccggcggac aaccgcgccg cgctcggcct ctactaccgg gacacgcgct tcctgtcctc
     2041 actcgagctg gcggtgaacg acctgcggcc gatcctgctg cactcatcga ccgagcggaa
     2101 ctacagccag atcgtggaga tggcgttccc gcaggtcgtg gccgacccgg cggggttcga
     2161 ggtcaaggag aacgtcggcg tctcccgcca gcgggtgctg tccgactcgc tcctggagcg
     2221 gatccggatc tcgaacttcg gccgccggcc gcagtcggtg cgcctgacga tggacttcgc
     2281 cgccgacttc ctcgacatct tcgaggttcg cgg
//
LOCUS       DASPWO010000053         7363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1030452, whole genome shotgun sequence.
ACCESSION   DASPWO010000053 DASPWO010000000
VERSION     DASPWO010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030452"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VEO00_01315"
     CDS             <1..796
                     /locus_tag="VEO00_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYU56673.1"
                     /translation="GLVWVRRMGFPRGRELAGAALFGVLSFGAFFSFVYLGLEGLPAG
                     AASVVMASVPLVTFVAAVLHRLERFRWRTLGGALVAIGGIAVMFGGGTSGELPLLRIG
                     SLLAAAICSAEAAVVAKRYRSVPPVVMNGVGMAVGALMLLGLTLAFREPLVAPSSRGT
                     WVALLYLVFLGSIATFITYLFVLRRWTATGASYAFVMAPIAAVVLAAWIEHEPVTSSL
                     VAGGALVLVGVYVGALSHGRRPAMAARERQTRPALAGLPADCQRCP"
     gene            complement(807..2414)
                     /gene="serA"
                     /locus_tag="VEO00_01320"
     CDS             complement(807..2414)
                     /gene="serA"
                     /locus_tag="VEO00_01320"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HYU56674.1"
                     /translation="MAEPLRVLITEPLSERGLDLLREEFAVDVRLDLAGTGLAEAIGP
                     YDALVVRSQTRVDAAVLEAGTNLKVVARAGIGLDNVDVETATRRGVMVVNAPQSNILS
                     AAEHTMALLLAQARNIPQANAALKAGRWERERFTGIELAGKTLGIVGVGRVGAMVAQR
                     ALAFGMRLIAFDPYISKERARQMGVELMPNLEALLVQADLVTVHLPLSSETRGLIGER
                     ELSLMKEGARIVNTARGGIVDEDALAKALKDGRLGGAALDVFSREPMTEHPLFEFDEV
                     VVTPHLGASTREAQDKAGTAIAEMVRLALRGEFVPYAVNVSAAAEVPGEVRPFLGLAE
                     RLGSILTGLAEGPMQAIEAEYLGRIAEHDTRVLTLAALKGCLSGVVHEPVSFVNAPVI
                     ARERGLAVGEKKSSVSRDYVNLITLRADTEAGEVAVAGTLVGKRDAERLVMVYDFDVD
                     MSPATHMAFFLYEDRPGVIGAVGTRLGDAAVNIASMEVARREQGGLALMGLTVDSPIP
                     DDVLDAIVASVGMERARSIVVAGPPSA"
     gene            complement(2423..3577)
                     /locus_tag="VEO00_01325"
     CDS             complement(2423..3577)
                     /locus_tag="VEO00_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HYU56675.1"
                     /translation="MTERPEILMTPGPTPVPPEVLLAQGSPLVYHRGPGFGALLREVM
                     DGLRGILRTSGDVLVFASSGTGGLESAVQNLLSPGDRVVVPVAGYFGERFANIAAAYG
                     AEVRRIDYEWGRATRPEDVAAALAEAPTRAVLMQHSETSTGVIHDVESVGGLTRDTDT
                     LLVVDVISSLGAVPYEGDAWGVDVAVGGAQKALMATPGLAFVSVNDRAWEARRTAKNP
                     RFYFDWATYEASASLDDPETPYTPAISLVAGLAAAIRLYLEEGMEAVLERHRVLSEAT
                     KEGVRALGLDLDGEFPERAWAVTAVRAPEGIDGNELVARVRARHRIILAPGQGPLKGK
                     VFRIGHLGYYDRFDIVRCLAALEITLEEMGYPVKRGAAVAAAESVFAANP"
     gene            4070..5554
                     /locus_tag="VEO00_01330"
     CDS             4070..5554
                     /locus_tag="VEO00_01330"
                     /inference="COORDINATES: protein motif:HMM:NF044948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU56676.1"
                     /translation="MDSPLPLTAHQPVEQARSRKRSTAVRAYVLAIVLPVVGFLVFSA
                     VTELDLRQELRSGAPWIIALFLVELFPLPVWREIRLSLGYPVIMLLAVLYTPAAVALF
                     VLLGAGDPKEFNGDWDLLRTAFNRSQVALAAFTASTVFHAFAPDRRLGPAVLAVGAVA
                     SATHLAVNMLSVGVAAKLDYRVPFREVFRRMLIGGSWAFEVAYAGLGVLGIVVALVYL
                     QVGTWAVVGLLPPLISMRYMLKAVGELQRAHATLERDAALQDVADQLAAERHQERQRL
                     AATLHDDLNPLLVSAGLRLDTVLARLDAVDHAAAEQIRTALAADRAAQATVRSLIGEL
                     LQSPTGPNGVSEGLRPLLSLLASRHGLAIRHYLEPIEAPATVNELLFGMAREAVRNAV
                     THGRAHLVEVSLREIEDVAVLTVADDGRGFEAERLGDDGQHYGLRILRERAEACGGRF
                     TVVSEPGSGTVVIGEIPLTPEATLQDTPPLDLARGPDGKVHLAL"
     gene            complement(6082..6435)
                     /locus_tag="VEO00_01335"
     CDS             complement(6082..6435)
                     /locus_tag="VEO00_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HYU56677.1"
                     /translation="MGTAIDSRQPSPGALIHSDHGTQFTSWTFTRRALDSGLIPSMGS
                     IGDCFDIAVVESFWARMQLELLDRRRWRTRVELANANFEYLEIFHTRRRRPSSLGMLT
                     PVEFERMHRERSEVA"
BASE COUNT          987 a         2610 c         2517 g         1249 t
ORIGIN      
        1 cggtctggtc tgggtccggc gcatgggctt cccgcgtgga cgcgagctcg ctggcgccgc
       61 gttgttcggc gtgctctcct tcggagcctt cttctcgttc gtctacctgg gcctggaggg
      121 cctgccggcc ggagcggcct ccgtcgtcat ggcatcggtg ccgctcgtca cgttcgtcgc
      181 ggcggtcctg caccggctgg agcggttccg ctggcggacg ctcggcggag cgctcgtcgc
      241 gatcggcggg atcgcggtga tgttcggcgg tgggaccagc ggcgagcttc cgctgcttcg
      301 gatcggctcg ctcctcgcgg cggccatctg ctccgccgag gcggccgtgg tggcgaaacg
      361 ctaccgctcg gttccgccgg tggtgatgaa cggcgtcggg atggcggtcg gcgcgctcat
      421 gctcctcggc ctcacgctgg cgttccgaga gccgctggtc gcgccctcct ccagggggac
      481 gtgggtggcc ctgctctatc tggtcttcct cggctcgatc gccaccttca tcacctacct
      541 cttcgtcctg cgccgctgga ccgcgacggg tgcctcctat gcgttcgtga tggcgccgat
      601 cgcagcggtc gtgctcgccg cctggatcga gcacgagccg gtcacctcca gcctggtcgc
      661 cgggggcgcg ctcgtgctgg taggcgtcta cgtcggggcg ctcagccacg ggcggcgccc
      721 ggccatggcg gcccgggagc ggcagacccg gcccgcgctg gccggcctgc cggccgattg
      781 ccagcgctgc ccctgagagt cgttccctac gccgacggcg ggccggcgac cacgatcgag
      841 cgggcgcgct ccatgccgac cgacgcgacg atcgcgtcga gcacgtcatc ggggatcggg
      901 ctgtccaccg tgagccccat cagcgccagc ccgccctgct cccggcgggc cacctccatg
      961 ctggcgatgt tcacggcggc gtcgccgagc cgcgtcccga ccgccccgat cacccccggc
     1021 cggtcctcgt acaggaagaa ggccatgtgg gtggccgggc tcatgtccac gtcgaagtcg
     1081 tagaccatca ccaggcgctc ggcgtcgcgc ttgccgacga gcgttccggc gacggccacc
     1141 tcgcccgcct ccgtgtcggc tcgcaacgtg atcaggttga cgtagtcgcg ggagacggag
     1201 gacttcttct cgccgaccgc caggccgcgc tcgcgcgcga tcaccggcgc gttgacgaac
     1261 gagaccggct cgtgcaccac gccggacagg cagcccttca gcgcggcgag cgtcaggacg
     1321 cgggtgtcgt gctcggcgat gcgcccgagg tactcggcct cgatcgcctg catcggcccc
     1381 tcggccagcc cggtcaggat cgagccgagc cgctcggcca gcccgaggaa cggccgcacc
     1441 tcgccgggca cctcggcggc ggccgacacg ttcaccgcgt agggaacgaa ctccccgcgc
     1501 agggccaggc ggaccatctc ggcgatggcg gtcccggctt tgtcctgcgc ctcccgcgtg
     1561 gacgcgccga ggtgcggcgt gacgacgacc tcgtcgaact cgaacagcgg gtgctcggtc
     1621 atcggctcgc ggctgaacac gtcgagcgcc gcgccgccga gccgcccgtc cttcagcgcc
     1681 ttggccaggg cgtcctcgtc cacgatcccg ccgcgcgcgg tgttcacgat ccgcgcgccc
     1741 tccttcatca gggacagctc ccgctcgccg atcagcccgc gcgtctcgga cgagagcggc
     1801 aggtggaccg tgaccaggtc cgcctgtacc agcagcgcct cgaggttcgg catcagctcc
     1861 acgcccatct gccgagcgcg ctccttcgag atgtaggggt cgaaggcgat caggcgcatc
     1921 ccgaacgcca gggcgcgctg cgccaccatc gccccgaccc ggccgacccc gacgatgccg
     1981 agcgtcttgc cggccagctc gatcccggtg aagcgctcgc gctcccaccg cccggccttc
     2041 agggccgcgt tcgcctgcgg gatgttgcgc gcctgggcca gcagcagcgc catggtgtgc
     2101 tcggccgcgg acaggatgtt cgactggggg gcgttcacca ccatcacgcc gcggcgggtg
     2161 gccgtctcga cgtccacgtt gtccaggccg atgccggccc gggcgacgac cttgaggttc
     2221 gtcccggcct cgagcaccgc ggcgtccacc cgggtctgcg accggacgac cagcgcgtcg
     2281 tacgggccga tcgcctcggc gaggccggtg ccggccaggt cgagtcgcac gtccacggcg
     2341 aactcctcgc gcaacaggtc gagcccccgc tcggaaaggg gctcggtgat caggacgcgg
     2401 agcggctcgg ccacggccag gcctacgggt ttgccgcgaa gaccgactcc gcggcggcga
     2461 cggcggcccc gcgcttcacc gggtagccca tctcctcgag cgtgatctcg agggcggcca
     2521 ggcagcggac gatgtcgaac cggtcgtagt agccgaggtg cccgatccgg aacaccttcc
     2581 ccttcagcgg accctggccg ggcgccagga tgatgcggtg ccgggcccgg acccgggcca
     2641 ccagctcgtt cccgtcgatc ccctcggggg cccgcaccgc ggtgaccgcc caggcgcgct
     2701 cggggaactc gccgtcgagg tccagcccca gcgcccggac gccctccttc gtcgcctcgg
     2761 acagcacgcg gtgccgctcg agcacggcct ccatcccctc ttccaggtac aggcggatgg
     2821 ccgcggcgag cccggcgacc agcgagatgg ccggcgtgta cggggtctcg gggtcgtcca
     2881 gtgaggcgct cgcctcatag gtcgcccagt cgaagtagaa gcgcgggttc ttcgccgtcc
     2941 gtctggcctc ccaagcccgg tcgttcacgc tgacgaaggc cagccccggg gtggccatca
     3001 gcgccttctg ggcgccgccg accgccacgt ccacccccca cgcgtctccc tcgtagggca
     3061 ccgcgccgag cgaggagatg acgtccacga ccagcagcgt gtcggtgtcg cgggtcaggc
     3121 cgccgaccga ctcgacgtcg tggatcacgc cggtcgaggt ctcgctgtgc tgcatcagca
     3181 ccgcgcgggt gggcgcctcg gccagggcgg cggccacgtc ctcgggccgg gtggccctcc
     3241 cccactcgta gtcgatgcgg cgcacctcgg cgccgtaggc ggcggcgatg ttcgcgaagc
     3301 gctcgccgaa gtagccggcc accgggacca ccacgcggtc gccgggggag agcaggttct
     3361 gcacggcaga ctccaggccg ccggtcccgg aggaggcgaa cacgagcacg tcgccagacg
     3421 tgcggaggat cccgcgcagg ccgtccatca cctcacgcag cagggccccg aaccccggcc
     3481 cgcggtggta gacgagcggg gacccctgcg ccaggagcac ttcgggcggg accggggtcg
     3541 gcccgggggt catcaggatc tcgggtctct cggtcaccgg ttcggggagc gtagcaccga
     3601 tctccgcgag caccgttccc tccgagggcg tcttcggccg ctcatccccc attcggccga
     3661 tcccttcgtg tggaccgttc gggcgattgc ggtgactagt tacctgtcct accttcaacc
     3721 gacgtccgga aggaggtgtg tggtgaaggg actgattcgc ttcgcgccct ggatgcggat
     3781 gctgccgatc gccctgctga tcgtcgcgat cctcgtcgct ggccgggcca actacaagtg
     3841 gtaggcaccg cctgcggccc ccctcgcgcc tgacgccctg gaaggaggtc tcccggtgag
     3901 gaagctcgcg cgcttcgcgc cccttctacg gatgctcccg atcgcgtcgc tcgtggtcgg
     3961 tatccttgta gcgggccagg cgaactacaa gtggtagggc gctagccgga ccctgccggc
     4021 cagcgcccca gaccacctcc caccgcccgc aaggaggatc acccggcacg tggattcgcc
     4081 acttcctctc accgcgcacc agccggtcga gcaggcacgc tcccggaagc gatcgaccgc
     4141 ggtccgggcc tacgtcctgg cgatcgtcct cccggtcgtc gggttcctgg tcttctccgc
     4201 ggtcaccgag ctcgacctcc gccaggagct ccgctccgga gcgccctgga tcattgccct
     4261 cttcctggtc gagctcttcc cgctcccggt gtggcgcgag atccgtctct ccctcggcta
     4321 cccggtgatc atgcttctgg cggtcctcta caccccggcc gccgtggcgc tgttcgtgct
     4381 cctgggcgcc ggtgacccga aggagttcaa cggcgactgg gacctgctgc ggacggcctt
     4441 caaccgctcg caggtcgccc tggcggcctt caccgcgtcg accgtgttcc acgcgttcgc
     4501 gccggacagg cgtctcgggc ctgccgtgct cgccgtcggc gcggtggcga gcgccaccca
     4561 cctggcggtg aacatgctgt ccgtcggcgt cgccgccaag ctcgactacc gggtgccgtt
     4621 ccgcgaagtg tttcggcgga tgctgatcgg cggctcctgg gcgttcgagg tggcctacgc
     4681 cgggctgggt gtgctcggga tcgtcgtcgc tctcgtctac ctgcaggtcg ggacgtgggc
     4741 cgtcgtcgga ctgctgccgc cgctcatctc gatgcgctac atgctgaagg cggtcggcga
     4801 gctccagcgg gcccacgcca cgctcgagcg cgacgccgcg ctccaggacg tcgccgatca
     4861 gctggccgcg gagcgccacc aggaacgcca gcgcctggcg gcgacgctgc atgacgacct
     4921 caacccgctc ctcgtgtcgg ccgggctccg cctggacacc gtcctggcga ggctcgacgc
     4981 cgtggaccac gcggccgctg agcagatccg caccgcgctg gcggccgacc gcgcggccca
     5041 ggcgacggtg cgctcgctga tcggcgagct gctccagtcg ccgaccgggc cgaacggcgt
     5101 gtccgaggga ctccgtccac tgctctccct gctcgcctcg cgacacggcc tggcgatccg
     5161 gcactacctc gagccgatcg aggccccggc aaccgtgaac gagctgctgt tcgggatggc
     5221 ccgcgaggcc gtgcgcaacg cggtcaccca cggccgggcc cacctggtcg aggtcagcct
     5281 ccgcgagatc gaggacgtgg ccgtcctgac cgtggccgac gacggccgtg ggttcgaggc
     5341 cgagcgcctc ggcgatgacg gccagcatta cggcctgcgg atccttcgcg agcgcgccga
     5401 ggcctgcggc ggccgcttca ccgtggtctc cgagcccggc agcgggaccg tggtcatcgg
     5461 tgagatcccg ctgacgcccg aggccaccct ccaggacacg cccccgctcg acctggcgcg
     5521 cgggccggac ggcaaggtcc acctggcgct gtagggcacc tccacgagag attccatccg
     5581 cgtgcgaaac gtagatcgca cggggcctgc cacgttcgtc ggcatcatgg tccctcgtac
     5641 gttgtcggca cgattccggg tggacagccg gctgctccag tcgccttttg ggttgggggt
     5701 gcacttggat gtgccgccgg agtcgtccat tccctcaatt cctgagatgt gagtggctat
     5761 gtttgaaccg ggggtattcg gaaggttggt gcgcatcgcg atcgctcttg gagtggtgtg
     5821 ggtgcttggc tatctcctga cggggagcac cttctgggct tcgttgtacg ttgggattgt
     5881 tgtactcacc ggaggtggat tcatcgtctg gctgctcatc agcaggaacc gccgaacgag
     5941 gtgattgagc tggaggggct cgcgacgctc tacgacgcgg ccgggcaccg cggtgcacca
     6001 cgaccggccc ggactgaacc gccccgggtt tcgtggagtg actggtgttc cccgggtttc
     6061 gtggagttag ctcgcttgag ttcatgccac ctctgaccgt tctctgtgca tcctctcgaa
     6121 ctcgaccggg gtgagcatcc cgagcgagct gggccgccgc ctgcgggtgt ggaagatctc
     6181 caggtactcg aagttcgcgt tcgccagctc gactcgggtc cgccaccgcc ggcggtcgag
     6241 cagttccagc tgcatcctgg cccagaacga ctcgaccacg gcgatatcga agcagtcgcc
     6301 gatcgacccc atggacggaa tgaggccgga atcgagcgcc cggcgggtga acgtccacga
     6361 cgtgaactgc gtcccgtggt ccgagtggat gagcgccccc ggcgagggct gccggctgtc
     6421 gatggccgtc cccaaggcgt tcgtatcgag cgaggacgtc tacatcgagt ggtcgtacgt
     6481 tgacgaagtg tgtgtttccg gagtgcccac tgggccacca ccccctcgga acgtcgaccc
     6541 agtggcccgg tactatcctc cccctcggca gattccctcg gcatgcgaag cgtagacctt
     6601 atatggtttg gtgcggttat gggtgaaatg gcgtcgtctg tgtacatcgg actcgccagt
     6661 cacgacaaac ggttccagct catgggatcc gctgggttgg caatcttggc ggtagcagtt
     6721 gctgtcgatg cgttgatgaa gtctagcgtg ggttactgga acggcttgct cgccctgatc
     6781 tccattccac cggcagccgt cctgatctca aagcgtggta aggagagctt ccgggcgatg
     6841 tcactacatt ctcaccgagt cctcggactg atcttcctat ccgcgatgct gttctcgctg
     6901 ctactgggcg tcgcgcttag aggagattga gttgagtcat ggagcggaat cgtaccccca
     6961 aggacaatga gcggcgacct ttggctgcgc tcagcatgtt tctggtcgtt gtcgctgcag
     7021 tcctcgtgct tcagagcgac tttccggtaa cttggaagct gctagaggtt gtgggtgccc
     7081 tgtgggtgct cgctgagacg tggcgttgga tgcattcgca gcgtcctttc tactctctct
     7141 tggccgatct cgcacggcgg ctcgtaaagg gttggaagag gtcataagag aaccgcccga
     7201 gttcagccca ggcttggttc tggagcgctt cgacgttcac ctacaacgac ctcggcgagc
     7261 gcctgaagat cgaagacggc gccggggtgg ggcggacatg gacgtacacg ccgcggggac
     7321 ttccggcgac ggcggccgac gcggccgcgc agactgggtt cca
//
LOCUS       DASPWO010000054         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_856404, whole genome shotgun sequence.
ACCESSION   DASPWO010000054 DASPWO010000000
VERSION     DASPWO010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856404"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VEO00_01340"
     CDS             <1..682
                     /locus_tag="VEO00_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYU56678.1"
                     /translation="TFDRPGFGGSDRREGRELLDTAADVDRLADHLGLDRFALVGISA
                     GGAHALACAHVLGDRLTAVGVASMPGPLDEVPGAWDALPTHVRPAAEMARREPARAIR
                     GVVRYMAPAVAEPATFLRGGPLADRALLADPEAGEMLLADVAEALRPGAEGFADDMVT
                     LWLPWGFLLAGLPEGIRIWHGAQDTRARPDFEHLAAALPGCVPAVWPDDGHYGVLRHW
                     GEVLARLR"
     gene            808..1392
                     /locus_tag="VEO00_01345"
     CDS             808..1392
                     /locus_tag="VEO00_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HYU56679.1"
                     /translation="MAQTGTYPVSFTVDYPDRELNRLTTAFRLILAIPILVVSAVLSN
                     SVGVLFLPPLLLILFRQKYPRWWFDWNLMILRFLTRIETYTFLLRDEYPSTDEEQAVH
                     LDIPYPDAKNELNRWLPLVKWLLAIPHIIVLAVLHLAVVVVTIIAWFAILFTGRYPRG
                     LFDFVVGVYRWTLRVTAYAFMLTTDRYPPFSLQA"
     gene            1427..1945
                     /locus_tag="VEO00_01350"
     CDS             1427..1945
                     /locus_tag="VEO00_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56680.1"
                     /translation="MAEGATATDAPAEPLNLAPIAAILVIAGPVLVAVSSFLEWAGRN
                     FKPAGKVLTFGGFEVPAKFVIDSKANLDGSGLPLGVLVLVAAAIALIGVLAPVPRARL
                     WIWVGGALATVIPLAYLVQLDDFMSRVNEAFPPGGFGAKNTVGFGAVLCGVGGVVTLA
                     GAALGWFAGRRS"
BASE COUNT          249 a          778 c          686 g          337 t
ORIGIN      
        1 cacgttcgac cggcccgggt tcggcggctc cgaccggcgt gagggtcgcg agctcctcga
       61 cacggccgcc gacgtcgatc ggctcgccga tcacctcggg ctcgaccggt tcgccctcgt
      121 cgggatctcg gccggaggcg cccacgcgct cgcctgcgcc cacgttctcg gcgaccgcct
      181 gaccgcggtg ggggtggcca gcatgccggg gccgctcgac gaggtcccgg gcgcgtggga
      241 cgccctcccc acccacgtcc ggccggcggc cgagatggcc cgccgcgagc ccgcccgggc
      301 catccgcggc gtcgtccgct acatggcgcc ggcggtggcc gagcccgcca ccttcctgcg
      361 gggcggtccc ctcgccgacc gggccctgct ggccgacccc gaggccggcg agatgctgct
      421 ggccgacgtg gccgaggccc tccggcccgg ggcggagggg ttcgccgacg acatggtcac
      481 cctctggctc ccgtggggct tcctgctcgc cgggctcccc gagggcatcc ggatctggca
      541 cggggcccag gacacccgag cccggcccga cttcgagcac ctcgccgccg ccctccccgg
      601 gtgcgtcccc gcggtgtggc ccgacgacgg ccactacggc gtcctccggc actggggcga
      661 ggtgctcgct cgactgcgct gagtgttcag acgaccgcgc ttaacgcgaa caccaacctg
      721 acggtgtcgc actgcacccg ggcccggcga ggccgtgtcg cgcgttcgcg cggcagctag
      781 cgtcctcccc gggctgagga ggcaatcatg gcgcagaccg gcacgtaccc cgtgagcttc
      841 accgtcgact accccgaccg cgagctcaac cgcctcacca ccgcgttccg cctgatcctg
      901 gcgatcccca tcctcgttgt gtcagcggtg ctctccaact cggtgggtgt cctgttcctg
      961 ccaccgctgc tgctgatcct gttccgccag aagtacccgc gctggtggtt cgactggaac
     1021 ctcatgatcc tgcggttcct cacccggatc gagacctaca cgttcctgct gcgcgacgag
     1081 tacccatcga ccgatgagga gcaggccgtc cacctcgaca tcccgtaccc ggacgccaag
     1141 aacgagctga accgatggct gccgctggtg aagtggctgc tcgcgatccc gcacatcatc
     1201 gtgctcgcgg tcctgcacct cgcagtggtc gtggtcacga tcatcgcctg gttcgcgatc
     1261 ctgttcaccg gccggtaccc gcggggactg ttcgatttcg tcgtcggcgt ctaccgctgg
     1321 acgctgcggg tgaccgcgta cgcgttcatg ctcaccaccg accgctaccc gccgttcagt
     1381 ctccaggcct gataagccgt cacgatgacc ctcaggagca cctgacatgg ccgaaggcgc
     1441 gactgccacc gatgcgcctg cggagccgct gaacctggcg ccgatcgcgg cgatcctcgt
     1501 catcgccggt ccggtgctcg tcgccgtctc cagcttcctc gagtgggcgg ggcgcaactt
     1561 caagccggcc ggcaaggtcc tgacgttcgg cggcttcgag gtaccggcca agttcgtgat
     1621 cgacagcaag gcgaacctcg acgggtccgg cctgcccctc ggcgtcctcg tgctggtcgc
     1681 agcggcgatc gcgctgatcg gcgtgctcgc ccccgtaccg cgcgctcgcc tgtggatctg
     1741 ggtgggcggc gcgctcgcca cggtcatccc gctggcgtac ctggtgcaac tggacgactt
     1801 catgtcgcgc gtcaacgagg cgttcccgcc cggcggcttc ggcgccaaga acacggtggg
     1861 cttcggcgcc gtcctgtgcg gcgtcggcgg cgtagtcacg ctcgccggcg ccgccctcgg
     1921 ctggttcgcc ggccgccgat cctgagacga gtacccgggc agatcgttgc gttcgtcggc
     1981 tcggctctgg tcgcgctgtc gacgccggcg cgctcacggc gcaggttcga tggctggcga
     2041 tcgtcggcgg
//
LOCUS       DASPWO010000055         2687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_856601, whole genome shotgun sequence.
ACCESSION   DASPWO010000055 DASPWO010000000
VERSION     DASPWO010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856601"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..489
                     /locus_tag="VEO00_01355"
     CDS             109..489
                     /locus_tag="VEO00_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56681.1"
                     /translation="MSVMVRAQPTVDVVRSGAHRGAELGREGAHFVAEMGRGAWEPIR
                     ERRERARRRERRGRMFRWGLIGLGGLAVGFLLGRVTANRGERASGDAELWEPTEPDWT
                     PERRSTPNADGRAPEVTLGERTPA"
     gene            complement(491..1456)
                     /gene="lipA"
                     /locus_tag="VEO00_01360"
     CDS             complement(491..1456)
                     /gene="lipA"
                     /locus_tag="VEO00_01360"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HYU56682.1"
                     /translation="MPGREHNPHGRRLYLPIVSTSSDPVPETAGAAGRRPEWLKVRAS
                     LGPNYRDLKGLMRGLDLHTICEEAGCPNIYECWEAREATFLILGDRCTRRCGFCDVMT
                     ARPGPVDEDEPARIAEAVRAMGLRHVVLTGVARDDLPDGGARIWAAAIRAVRDAVPGI
                     GIEVLPADFQGGEHDIATVVGAEPDVFAHNLETVRHLHGRIRPAFGYDRSLEVLRLAK
                     GLRPGQVTKSNLILGMGEAAEEVEEAMRDLAATGCDLLTIGQYLQPTRMHLPVDRWVT
                     PEEFAAHARAGMAMGFAHVEAGPLVRSSYHAGRQLSRARGALSAR"
     gene            1637..2044
                     /locus_tag="VEO00_01365"
     CDS             1637..2044
                     /locus_tag="VEO00_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYU56683.1"
                     /translation="MRRTMAVGVVFLLVVGLLAIAGSVSAGGTITCGGFATTLEGTSG
                     DNILIGTDANDVIHGRGGDDAIFGRGGRDELCGGGGNDTVSGMGANDNVRGNSGNDDL
                     FGGGGRDHLNGDGGTDFCDGGNGTDVLESCERS"
     gene            complement(2087..2635)
                     /locus_tag="VEO00_01370"
     CDS             complement(2087..2635)
                     /locus_tag="VEO00_01370"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HYU56684.1"
                     /translation="MLVEGDRILLRGHERADAAALADILTRNRVFFRPFEPERPERLL
                     TVRGQQEDIARWQREWRNDGAYAFVVALREGGTIVGRVALTNVVRFAWLNANLGYFID
                     QDHNGRGYGTEAVRLATRFAFRDAGLHRLQAAVRVDNARSIRVLEKAGYRREALAERY
                     LFIDGAWRDMYLYAQTAEELLG"
BASE COUNT          384 a          945 c          996 g          362 t
ORIGIN      
        1 cttacaggcc ggctcctcca cctctccacc ccccgctcct ccggcctggc gtctccttgg
       61 gttgcggcgg cctcccccgg ggtatggcac cgggcggagg tgaggccgat gtcggtcatg
      121 gtgagggcac agcccacggt cgacgtggtc cgctccggcg cccatcgtgg ggccgagctg
      181 ggccgtgagg gtgcgcactt cgtggcggag atgggccgcg gcgcctggga gccgatccgg
      241 gagcgtcgcg agcgggcccg gcggcgagaa cgccgcggac ggatgtttcg ctgggggttg
      301 atcgggctgg gcggcctggc cgtcgggttc ctgctgggtc gggtcaccgc gaaccgcggc
      361 gagcgggcga gcggcgacgc cgagctctgg gagccgacgg agcccgactg gaccccggag
      421 cgccggtcca cgccgaacgc ggacggccgg gcgcccgagg tgaccctcgg cgagcggacg
      481 ccggcctgat tcagcgggcc gagagcgccc cgcgtgcccg cgagagctgc cggccggcgt
      541 ggtaggagga ccgtaccagc gggccggctt cgacgtgcgc gaaccccatc gccatcccgg
      601 cgcgggcgtg cgccgcgaac tcctccggcg tgacccaccg atcgaccgga aggtgcatcc
      661 gcgtcggctg caggtactgg ccgatggtca agaggtcgca gccggtcgcg gcgaggtcgc
      721 gcatcgcctc ctcgacctcc tccgcggcct cgcccatgcc gaggatcagg ttcgacttcg
      781 tcacctgacc cggccggagg cccttggcga gacgcagcac ctcgagcgag cggtcgtagc
      841 cgaaggccgg gcggatgcgc ccgtgcaggt ggcggacggt ctcgaggttg tgggcgaaca
      901 cgtccggctc cgccccgacc accgtggcga tgtcatgctc cccgccctgg aagtcggcgg
      961 gaaggacctc gatcccgatc ccgggcaccg cgtcgcgcac ggcgcggatc gccgccgccc
     1021 agatccgcgc cccgccgtcc ggcagatcgt cgcgggcgac cccggtcagc acgacgtggc
     1081 gcagccccat ggcgcggacc gcctcggcga tccgggcggg ctcgtcctcg tcaaccgggc
     1141 ccggccgggc ggtcatcacg tcgcagaagc cgcagcggcg cgtgcagcgg tcgccgagga
     1201 tcaggaacgt cgcctcgcgc gcctcccagc actcgtagat gttcgggcag cccgcctcct
     1261 cgcagatggt gtggaggtcc aggccgcgca tcaggccctt caggtcgcgg tagttcggcc
     1321 cgaggctcgc ccgcaccttc agccactcgg gacggcgacc ggcggccccc gccgtctccg
     1381 ggaccggatc ggaggaggtg gagacgatcg gaagatagag gcggcgcccg tggggattgt
     1441 gctcgcgtcc cggcatcccg ctcagtctac ggccgccgcg atcgaacccc gagcgcgctc
     1501 gagcacgcat gtgaggttga accggaccag cccatccggg ccacacaggc cgaatccctt
     1561 gtgccggatc ggtccgcggt gtcatgatcg tggcacggcc gagaagcccg gccggtcgag
     1621 ggggaaggag gtcggcatgc gaaggaccat ggcggtgggg gttgtcttcc tcttggtggt
     1681 cgggttgctc gcgatcgcag gcagcgtctc ggcgggcgga accatcacct gtggcggctt
     1741 cgcaacgacg ctggagggaa cgagcggaga caacatcctg atcggcaccg atgccaacga
     1801 cgtcatccac ggtaggggtg gtgacgacgc catcttcggt cgcggcggcc gcgacgagct
     1861 ctgtggcggc ggcggcaacg acaccgtgtc ggggatgggc gccaacgaca acgtcagggg
     1921 caacagcggg aacgacgatc tgttcggcgg cggaggccgg gatcacctga acggcgatgg
     1981 cggcaccgac ttctgcgatg gcggcaacgg gaccgacgtc ctggaaagct gcgagcggag
     2041 ctagggacag cgcagtaccg ccagatgccc cgaagggctc cccgggtcac ccgaggagct
     2101 cttcggctgt ctgggcgtac aggtacatgt cgcgccaggc gccgtcgatg aacaggtagc
     2161 gctcggccag cgcctcgcgc cggtagccgg ccttctccag cacccggatc gaccgggcgt
     2221 tgtcgacgcg caccgcggcc tgcaggcggt gcagccccgc atcgcggaac gcgaagcggg
     2281 ttgcgaggcg gaccgcctcg gtgccgtacc cccggccgtt gtggtcctgg tcgatgaagt
     2341 agcccaggtt cgcgttcagc caggcgaacc gcaccacgtt cgtcagggcc acgcggccga
     2401 cgatcgtccc gccctcgcgg agcgcgacca cgaacgcgta ggcgccgtcg ttccgccact
     2461 cgcgctgcca gcgggcgatg tcctcctgct gcccgcgcac cgtcagcaac cgttccgggc
     2521 gctccggctc gaagggccgg aagaacacgc ggttgcgggt caggatgtcg gccagcgcgg
     2581 cggcgtcggc tcgttcgtgg ccgcgaagca ggatccgatc gccctccacc agcatccggc
     2641 gatgatcccg ggctagaacc gggccagctc ccgcatccgc tcgagga
//
LOCUS       DASPWO010000056         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_431169, whole genome shotgun sequence.
ACCESSION   DASPWO010000056 DASPWO010000000
VERSION     DASPWO010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431169"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VEO00_01375"
     CDS             complement(<1..222)
                     /locus_tag="VEO00_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56685.1"
                     /translation="MSARHGVAYRLSAPLRFLWALPGSVIGFLLGAFSFALPRYDEGT
                     LVFESRVGFAALLKRVGYTAITLGQVIVVH"
     gene            complement(219..1232)
                     /gene="trpD"
                     /locus_tag="VEO00_01380"
     CDS             complement(219..1232)
                     /gene="trpD"
                     /locus_tag="VEO00_01380"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HYU56686.1"
                     /translation="MSEWPALLSRLLAGEALSAGDAHAAMGAIMDGEATQAQIAGFVV
                     ALRAKGETPEEIEGLARAMLERAQPVETPGPVVDTCGTGGDRAGTINVSTVAALVVAG
                     AGVTVAKHGNRAASSRCGSADLLEALGVKIDLGPDGVARCLAEAGIGFMFASVYHPAM
                     AHAAVPRRELGIPTVFNFLGPLTNPARPAAQVVGVSDPRMLPKLAMVLARRGTRAMVV
                     RGADGLDELTVTGPSDVHDVSAGVVRSVTVDPASLGLPTHPPGAVAGGDAEHNAAVAR
                     AIVAGEPGPSRDIVLLNAAAVLTVAGVAPSIEEGLGAAAESIDAGRAAAALTRLIETS
                     NAP"
     gene            complement(1229..1564)
                     /locus_tag="VEO00_01385"
     CDS             complement(1229..1564)
                     /locus_tag="VEO00_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU56687.1"
                     /translation="MAIEIEASRLLIYRAAAMADRGEVRREAAGHLAASKALPSEMAE
                     RVASAGLQILGARGYSTAHPLERAYRDAKQLQIVEGTSQVQRLIIGRAVLGGDLGYGG
                     WHGPEEPRP"
     gene            complement(1531..>2094)
                     /locus_tag="VEO00_01390"
     CDS             complement(1531..>2094)
                     /locus_tag="VEO00_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU56688.1"
                     /translation="ARWGGPVARGATRMAFCLTEPGAGSDVAAMTSRAERGGDGYRLT
                     GRKSWISQYREAGAFSVFARTGDDGAVSCFVVPRDAPGVSLEEPEGKMGVRGVATGDV
                     AFDRTPVPAGDRVGEEGAGFRIALQALNATRPVIGARGAGLAEGVLEYARAYALERRA
                     FGGRLSDLQWVCSRWPTWRSRSRRRAC"
BASE COUNT          279 a          853 c          732 g          230 t
ORIGIN      
        1 gtggaccacg atgacctgcc cgagcgtgat cgccgtgtag ccgacgcgct tcaagagcgc
       61 cgcgaacccc acccggctct cgaacaccag ggtcccctcg tcgtagcgcg gcagcgcgaa
      121 cgagaacgcc ccgagcagga acccgatgac cgaccccggc agcgcccaca gaaaccgcag
      181 cggcgccgac aggcggtacg cgacgccgtg ccgcgcgctc acggcgcgtt ggaggtctcg
      241 atcaagcggg tcagggcggc ggcggcgcgg ccggcatcga tcgactcggc ggcggcgccc
      301 aggccctcct cgatcgaggg ggccacgccg gcgacggtca ggacggccgc ggcgttcaag
      361 agcacgatgt cgcgcgacgg gccgggctct ccggccacga tcgcgcgggc cacggcggcg
      421 ttgtgttcgg cgtcgccgcc ggccacggca cccggcggat gggtcgggag gcccagggag
      481 gccggatcca ccgtcaccga ccggaccacg ccggcggaga cgtcgtggac gtcgctcggg
      541 ccggtgacgg tcagctcgtc gagcccgtcg gcgccgcgga cgaccatcgc ccgggttccc
      601 cggcgcgcca ggaccatcgc cagcttcggc agcatccggg ggtccgacac gccgaccacc
      661 tgcgccgccg ggcgagcggg gttcgtcagc ggccccagga agttgaagac ggtcgggatg
      721 cccagctcgc ggcgcggcac cgccgcgtgc gccatcgccg ggtggtagac ggacgcgaac
      781 atgaacccga tcccggcctc ggccaggcag cgcgccacgc cgtcgggtcc gaggtcgatc
      841 ttcaccccga gcgcctcgag caggtccgcc gagccacagc gggaggacgc ggcgcggttc
      901 ccgtgcttcg ccacggtgac cccggcgccg gcgaccacga gcgccgccac cgtggacacg
      961 ttgatcgtcc cggcgcggtc gccgccggtg ccgcacgtgt cgaccaccgg ccccggcgtc
     1021 tccaccggct gcgcccgctc gagcatggcc cgggccagcc cctcgatctc ctccggggtc
     1081 tcccccttcg cccgcagcgc cacgacgaag cccgcgatct gggcctgcgt ggcctcgccg
     1141 tccatgatcg cccccatggc ggcgtgggcg tcgccggccg agagcgcctc gccggccagg
     1201 aggcgcgaca gcagcgccgg ccactcgctc acggccgcgg ctcctccggg ccgtgccaac
     1261 cgccgtagcc gaggtcgccg cccagcacag ctcgcccgat gatcaggcgc tgcacctggc
     1321 tggtcccctc gacgatctgc agctgcttcg cgtcgcggta ggcccgctcc aggggatgcg
     1381 cggtggagta cccgcgggca ccgaggatct gcagcccggc cgaggcgacc cgctcggcca
     1441 tctccgacgg gagagccttg ctcgcggcga gatgcccggc cgcctcccgg cgcacctccc
     1501 cgcggtccgc catcgccgcc gcccggtaga tcagcaggcg cgacgcctcg atctcgatcg
     1561 ccatgtcggc cagcgtgaac acacccactg caggtccgac agccggccgc cgaaggcgcg
     1621 tcgctccagc gcgtaggcac gggcgtactc gaggacgccc tccgccagcc cggccccgcg
     1681 cgcgccgatc accgggcgcg tggcgttcag cgcctgcagc gcgatccgga agccggcgcc
     1741 ctcttccccg acgcggtccc ccgccggaac cggggtccgg tcgaacgcca cgtcgccggt
     1801 ggccacgccg cgcacgccca tcttcccctc cggctcttcg agcgagacgc ccggcgcgtc
     1861 gcgcggaacc acgaagcagc tcacggcgcc gtcgtccccg gtgcgggcga acaccgagaa
     1921 cgcgccggcc tcccggtact gcgagatcca ggacttccgg ccggtcagtc ggtagccgtc
     1981 gccgccccgc tcggcgcgcg acgtcatcgc cgccacgtcc gagccggcac cgggctcggt
     2041 caggcagaag gccatccgcg tcgccccccg cgccaccggc ccgccccacc gcgc
//
LOCUS       DASPWO010000057         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_264870, whole genome shotgun sequence.
ACCESSION   DASPWO010000057 DASPWO010000000
VERSION     DASPWO010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264870"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1093)
                     /locus_tag="VEO00_01395"
     CDS             complement(155..1093)
                     /locus_tag="VEO00_01395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HYU56689.1"
                     /translation="MRAFVVEEFGKPGSVREVPDPRPAPDEVLVRVRAGGVNPADWAM
                     VMGMMKQYAEHRFPLIPGIDLSGEVVEAPGDVALAAGDDVYGAVGKPYYGEGSWAELV
                     AVAVGDVAVKPAALDHVAASGVPLAGLTALQAVEAADVVEGGRLLVVGATGGVGSYAV
                     QIAAIRGAQVVAVSRGENAAYARELGAAETIDYTQGDLADRVRAAHPDGVDALIDLAS
                     DGDAVLRLAEVVREGGAVVSARGAANPETLESRGRRGVNVSRAPLARLPELSALFDDA
                     RLFAPPTRTFPLDRAQDAIDEVATGHVRGKLALTIP"
     gene            complement(1254..>2036)
                     /locus_tag="VEO00_01400"
     CDS             complement(1254..>2036)
                     /locus_tag="VEO00_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HYU56690.1"
                     /translation="RVLPTERPDTWEVQGRGELQLAVLVEQMRREGFELTVGKPQVVI
                     RHVDGKVHEPVERVAIDVPEDYIGVVTQLLALRKGALQQLVNHGTGWVRMEYLVPARG
                     LIGFRTEFLTETRGTGLLHHVFEAYEPWHGDLRTRPTGSLVADRRGLTTSFALLNLQE
                     RGSVFVGPGVEVYEGMIVGENSRSEDMNVNPAKEKKLTNIRSSTAEELVRLIPPRELS
                     LEQALEFIREDEAVEVTPKNVRLRKAVLDAGERVKATRRTPS"
BASE COUNT          270 a          757 c          722 g          287 t
ORIGIN      
        1 tccaggtgcg ccagggcctc cacaccggag agtgcacgtt cgccgacggc gacgtcagcg
       61 gcatcgccgt cgccatcgcc tcccgggtca tggcgctggc cggcgagcac atgctgaggg
      121 gcgtccccga cacctggcgc ctgttcgcgg cccgctaggg gatcgtcagg gccagcttcc
      181 cgcggacgtg gccggtggcg acctcgtcga tcgcgtcctg cgcccggtcc agcgggaagg
      241 tccgggtcgg cggggcgaac aggcgggcgt cgtcgaacag cgcgctcagc tcgggcaggc
      301 gcgccagcgg ggcgcggctg acgttgacgc cgcgccggcc gcgcgactcg agggtctcgg
      361 ggttcgcggc gccgcgggcc gacaccacgg cgcctccttc gcggaccacc tcggcgaggc
      421 gcaggacggc gtcgccgtca ctcgccaggt cgatcagcgc gtccacgccg tcggggtgcg
      481 cggcgcggac ccggtccgcc aggtcgccct gggtgtagtc gatggtctcg gcggcgccca
      541 gctcgcgcgc gtaggcggcg ttctccccgc gcgagacggc caccacctgc gcgccgcgga
      601 tcgccgctat ctgcaccgcg tagctcccga cgccgccggt cgccccgacc acgagcaggc
      661 gcccgccctc cacgacgtcg gcggcctcga cggcctggag cgccgtgagc ccggccaggg
      721 gaacgccgga ggccgcgacg tggtcgagcg ccgccggctt gacggccacg tcgccgacgg
      781 cgacggcgac gagctcggcc cacgatccct cgccgtagta cggcttgccg acggccccgt
      841 acacatcgtc gccggcggcg agcgccacgt cgccgggcgc ctcgaccacc tcgccggaca
      901 ggtcgatgcc ggggatcagg gggaagcggt gctcggcgta ctgcttcatc atccccatca
      961 ccatggccca atcggccggg ttcacaccgc cggcgcgcac gcgcaccagg acctcgtcgg
     1021 gggcgggacg gggatcgggc acctcgcgca ccgacccggg cttcccaaac tcctcgacaa
     1081 cgaacgcgcg cactacggtc tcctttcgag aggttgcctc tggacaaccg ctcccctggc
     1141 gaccgtattc cgctgcactc acaggcggaa ctcgaggagc tcgttcgcgg cgaaggccgg
     1201 gacccagagg gtgtcctcga tgaagaaggg ggtgcccggc tccgcggggc gggtcagctc
     1261 ggggttctgc gggtggcctt gacgcgctcg ccggcgtcga ggacggcctt gcgcagccgg
     1321 acgttcttcg gggtgacctc gacggcctcg tcctcgcgga tgaactcgag cgcctgctcg
     1381 agcgacagct cgcgcggcgg gatcaggcgc accagctcct cggccgtcga ggatcggatg
     1441 ttcgtcagct tcttctcctt ggcggggttg acgttcatgt cctccgaacg gctgttctcg
     1501 ccgacgatca tgccctcgta gacctcgacg cccggcccga cgaacacaga gccgcgctcc
     1561 tgcaggttga gcaacgcgaa gctcgtggtc aggccgcggc ggtcggcgac gagcgagccg
     1621 gtcgggcgcg ttcgcaggtc gccgtgccac ggctcgtacg cctcgaacac gtggtgcagc
     1681 agcccggtgc cgcgcgtctc ggtcaggaac tccgtgcgga acccgatcag gccgcgcgcg
     1741 gggaccaggt actccatccg cacccagccg gtgccgtggt tgaccagctg ctggagcgcg
     1801 ccctttcgaa gcgccagcag ctgggtgacg acgccgatgt agtcctccgg cacgtcgatc
     1861 gcgacgcgct cgaccggctc gtgcaccttc ccgtccacgt gccggatcac gacctgcggc
     1921 ttgccgacgg tgagctcgaa gccctcgcgc cgcatctgct cgacgagcac cgcgagctgg
     1981 agttcgccgc gtccttgcac ctcccaagtg tccggacgct ccgtcggaag cacgcg
//
LOCUS       DASPWO010000058         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_264917, whole genome shotgun sequence.
ACCESSION   DASPWO010000058 DASPWO010000000
VERSION     DASPWO010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264917"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /gene="metK"
                     /locus_tag="VEO00_01405"
     CDS             complement(<1..853)
                     /gene="metK"
                     /locus_tag="VEO00_01405"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HYU56691.1"
                     /translation="MTEGHLFTSESVTEGHPDKLADQISDAVLDAIMAEDPNGRVACE
                     TLITTGLVVVAGEITTETYVDVPKVVRETITGIGYTDSKFGIDGETCGVVTAIQEQSA
                     DIAQGVEHALEERVEHSHDDLDTLGAGDQGMMFGFACTETEELMPLPIAMAHRLAHRL
                     ADVRKAGVVPYLRPDGKSQVTIEYDGERPVQVHTVLISAQHKEEVDLETLLASDVKEY
                     VIDPVLHEYALDAPDVRVLVNPTGRFETGGPKADTGLTGRKIIVDSYGGMAPHGGGCF
                     SGKDPTKV"
     gene            945..2507
                     /locus_tag="VEO00_01410"
     CDS             945..2507
                     /locus_tag="VEO00_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56692.1"
                     /translation="MASVGDAGDVRQFVGSLFTDPELRHAFREDPATVLDERGLADRP
                     LTEFRDALHEATDRLPADVAERFGAFQRRVERVLAGDVADGPSATTDEAAEPPAGEAP
                     PETPAVANVPIPGIEVESTGLDLGIDPDVLDTAPPEPILTAPAPAATPQPSPELQAVR
                     TAADIGTSAADAVRTAAAHIPQEPADQLGELATGIGEDVANEIADELRQFQDDLAEEF
                     GIDPDDIPVDVPALVEKIGQGDVEGAAEDLARAAADQVIGVPGAVELGEQVLEGDFEG
                     AAGAAARAAARTAGVPEEGIEIGEKLAEGDVGGAAEDAGREAAYAAAESLGVSRDVVD
                     VGEDIVRGEADAEDVASAAGRAGAEAVGAGGYVEAGEDFLAGDYTQGALGAVGTTIGY
                     AFGGPLLSTVGGAVGEGLGEALDYIGANDLIESGAEWVGGAAVDLVTDPLGTIEVAAE
                     DIGDLILAAPDVFVGAAESVGEGIGDAPDVFVDAAESVGEGIGDAAESVGEGIGDAAE
                     AVGDVLDDLNPF"
     gene            2521..3945
                     /locus_tag="VEO00_01415"
     CDS             2521..3945
                     /locus_tag="VEO00_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56693.1"
                     /translation="MAATEESADVRRFVRSLFADRDLRQEFRENPAALLEERGLSGVS
                     VTEFRDALRDLSDRVPMDVAERFGPVLSHLDDVVAGNDGAEKPVEAPGPAAGAGPEIP
                     AAPAPIDPVLPGERTSSPDDPTALAPTPEVGMIADPAPGADLPGDLATGGFTGARDVL
                     GQPGDNAAGEAAAGVGDLAGELGLDPDLGVDAAAIGEAVLGGDLGGAAEEAARAAAGE
                     VADELGVPGEVLGVGEKLAEGDAEGAAEEAARAAAQAAAEELGVPGGAVDVGEGLLSG
                     DGVSAEDAALLAAEIAAEAAGVGGYVRAGEDLIEGDYTQAYFGTVGTTIGELFGPAGG
                     TVGGLIGKGFGEVLDYFGANDAIDAGATLVFDWGTEAAEDLVTDPFGTTEMAVTDTVD
                     FVLDAPALVLDTAGDVLDTAGGVAEDVGGAIGDAGEAAGDAAGDALAAVGDAAGDVAE
                     GAGGVVEDAGGVVSDVGDALDDLF"
BASE COUNT          550 a         1483 c         1644 g          541 t
ORIGIN      
        1 cgaccttggt cgggtccttc ccggagaagc agcctccgcc gtgcggggcc atcccgccgt
       61 aggaatcgac gatgatcttg cgcccggtca gcccggtgtc ggccttcggc ccgccggtct
      121 cgaaccggcc ggtcgggttg acgaggacgc gcacgtcggg agcgtccagg gcgtactcgt
      181 ggagcaccgg gtcgatgacg tactccttca cgtcggacgc cagcagcgtc tccaggtcga
      241 cctcctcctt gtgctgcgcg gagatcagga ccgtgtgcac ctgcaccggc cgctcgccgt
      301 cgtactcgat cgtcacctgc gacttgccgt ccgggcgcag gtaggggacc acgccggcct
      361 tgcgcacgtc ggcgagccgg tgggccagcc ggtgggccat cgcgatcgga agcggcatca
      421 gctcctcggt ctcggtgcag gcgaagccga acatcatccc ctggtcaccg gcgccgagcg
      481 tgtcgaggtc gtcgtgggag tgctccaccc gctcctcgag cgcgtgctcg accccctggg
      541 cgatgtcggc ggactgctcc tggatcgccg tgaccacgcc gcaggtttcc ccgtcgatcc
      601 cgaacttcga gtccgtgtag ccgattccgg tgatcgtctc gcggaccacc tttgggacgt
      661 ccacataggt ctcggtcgtg atctcgccgg cgacgacgac caggccggtg gtgatcagcg
      721 tctcgcaggc gacgcggccg ttcgggtcct ccgccatgat cgcgtcgagc acggcgtcgg
      781 agatctggtc ggcgagcttg tcggggtgtc cctcggtgac cgactccgag gtgaacaggt
      841 ggccctccgt catgacggcc gattataggg gccggggccg acgccacccc tttacgaacc
      901 gccgggttgc gcctactgtc acgccaccgc acgaggaggc gctcatggcc agcgtcggcg
      961 acgccggcga cgtccgacag ttcgtcggtt cgctcttcac agaccccgag ctccgccacg
     1021 cgtttcggga ggatccggcg acggttctcg acgagcgggg gctggccgac cgaccgctga
     1081 cggagttccg cgacgcgctg cacgaggcaa ccgatcgcct gccggcggac gtggccgagc
     1141 ggttcggggc gttccagcgc cgggtcgagc gggtgctggc cggggacgtc gccgacggcc
     1201 cgtccgcaac caccgacgag gcggccgagc cgccggccgg cgaggcgcca ccggagaccc
     1261 cagccgtcgc caacgtgccg atcccgggga tcgaggtcga atcgaccggc ctcgacctgg
     1321 gaatcgatcc cgacgtcctg gacaccgcgc cgccggagcc gatcctgacc gcgccggcgc
     1381 cggcggccac cccccagccg agccccgagc tgcaggcggt ccggacggcc gcggatatcg
     1441 gcacgagcgc cgcggacgcc gtgcggacgg ccgccgccca catcccccag gagccggccg
     1501 accagctcgg cgaactcgcc accggcatcg gcgaggacgt cgcgaacgag atcgccgacg
     1561 agctccgtca gttccaggac gacctcgccg aagagttcgg catcgacccg gacgacatcc
     1621 ccgtcgacgt ccctgcgctg gtcgagaaga tcggccaggg ggacgtcgag ggcgcggcgg
     1681 aggacctcgc gcgcgccgcc gccgatcagg tgatcggggt gcccggcgcg gtcgagctcg
     1741 gcgagcaggt cctggagggc gacttcgagg gcgcggccgg ggcggccgcc cgggcggcgg
     1801 ccaggacggc cggcgtgccg gaggaaggga tcgagatcgg cgagaagctg gcggaggggg
     1861 acgtcggcgg ggcggccgaa gacgccggcc gagaagccgc atacgccgcg gcggagtcgc
     1921 tgggcgtctc ccgggacgtg gtggacgtcg gcgaggacat cgtccggggc gaggccgacg
     1981 ccgaggacgt cgcgagcgcg gcgggccgcg ccggagcgga ggcggtgggc gccggtgggt
     2041 acgtcgaggc cggcgaggac ttcctggcgg gcgactacac ccagggcgcc ctgggcgcgg
     2101 tgggcacgac gatcggctac gcgttcgggg gcccgctgct ctccacggtc ggcggcgcgg
     2161 tcggcgaggg cctcggcgag gcgctcgact acatcggggc gaacgacctg atcgagtcgg
     2221 gtgccgagtg ggtgggcggg gccgccgtcg acctcgtcac cgatccgctc gggacgatcg
     2281 aggtggcggc ggaggacatc ggtgacctca tcctggccgc cccggatgtc ttcgtgggcg
     2341 cggcggagtc ggtcggcgag ggcatcggcg acgcccctga tgtcttcgtg gacgcggcgg
     2401 agtccgtggg cgagggcatc ggcgacgccg ccgagtccgt cggcgagggc atcggcgacg
     2461 cggccgaggc ggtcggcgac gttctcgatg acctcaaccc gttctgacga ggaagacgac
     2521 atggccgcaa ccgaggaatc cgccgacgtc cgccgattcg tccggtcgct gttcgccgac
     2581 cgcgacctgc gccaggagtt ccgggagaac ccggcggcgt tgctcgagga gcgcgggctc
     2641 tccggcgtga gcgtcaccga gttccgcgac gcgctgcgtg acctgtccga ccgcgtgccg
     2701 atggacgtcg ccgagcgctt cggcccggtg ctgtcacacc tggacgacgt ggtcgcgggg
     2761 aacgacggtg cggagaaacc cgtcgaggcg ccgggtccgg cagccggcgc cgggcccgag
     2821 atcccggctg cccctgcccc gatcgatccg gtgctccccg gcgagcggac gagctcgccg
     2881 gacgatccga cggccctcgc tcccaccccc gaggtcggga tgatcgccga ccccgcaccg
     2941 ggcgcggacc tccccggcga cctcgccacc ggcggattca ccggcgctcg cgacgtcctg
     3001 gggcagcccg gggacaatgc ggccggcgag gccgcggcgg gtgtcgggga tctcgccggc
     3061 gagctcggtc tggatcccga cctcggcgtc gacgcggccg cgatcgggga ggcggtcctc
     3121 ggcggcgacc tgggcggcgc cgccgaggag gcggcccggg cggccgcggg cgaggtggcc
     3181 gacgagctcg gcgtgcccgg cgaggtgctc ggggtcggcg agaagctcgc cgagggcgac
     3241 gcggaggggg cggccgagga agccgcgcga gcggccgctc aagccgcagc cgaggagctc
     3301 ggggtcccgg gcggggccgt cgacgtcggc gagggcctgc tctccggcga cggggtctcc
     3361 gccgaggacg ctgccctcct ggccgccgag atcgccgccg aggcggccgg cgtgggcggc
     3421 tacgtgcggg cgggcgagga cctgatcgag ggcgactaca cacaggccta cttcggcacc
     3481 gtcgggacca cgatcggcga gctgttcggc ccggccggcg gaaccgtcgg cggactgatc
     3541 gggaaggggt tcggcgaggt cctcgactac ttcggcgcga acgacgccat cgacgcgggc
     3601 gccacgctgg tcttcgactg gggaaccgag gcggccgagg acctggtcac cgacccgttc
     3661 gggaccaccg agatggccgt caccgacacg gtggacttcg tcctcgatgc gcccgccctc
     3721 gtgctcgaca cggccggcga cgtcctcgac acggccggag gcgtcgcgga ggacgtcggc
     3781 ggggcgatcg gagacgccgg ggaggccgcc ggggatgccg ccggcgacgc gctcgcggcg
     3841 gtcggggacg cggccggcga cgtcgccgag ggcgccggcg gggtggtcga ggacgccgga
     3901 ggggtcgtct cggacgtcgg cgacgcgctg gacgacctct tctgatcggc gggaccgacc
     3961 cgggacgtcc cgggaccctg cacggcttgt ctccctcgga tcgcggtggc agagtctcgt
     4021 tctccgccgg ccgatcccgg cgggaggtcg tcatggaggc cgactccccg catctccagc
     4081 gcctgctcga acggcacggc gacctggccg cgcggccgct tcgcgcggcg attatcggcc
     4141 ccggcggctc cgggaagacc gagctgctgg cgcgcctctc cgaagggctg gccatggccg
     4201 gcgtgagggt ggtcccgg
//
LOCUS       DASPWO010000059         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_179992, whole genome shotgun sequence.
ACCESSION   DASPWO010000059 DASPWO010000000
VERSION     DASPWO010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179992"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1785)
                     /locus_tag="VEO00_01420"
     CDS             complement(<1..1785)
                     /locus_tag="VEO00_01420"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HYU56694.1"
                     /translation="MSEPAREPQPAGGPEFPGIRETATTVLVTDLASKVLATKEGETF
                     LYSDTEGNLDDRRELGLGFYHQDTRFLSRFLMRLSGREPVLLSSSAERAYMSYVDLTN
                     PDLFEGDQLAVPQQTLNVRRIRTINGRLFERIRVKNYNPYPVSVQLEFSLGADFADVF
                     EVRGLARDVRGHYEPPRADGRIVEFAYRGMDGVVQRSVVEFGSDPTETEIRGDVVVVR
                     FALRLAAHQTRLVSLTVEPVNGQTRPHALEFDVAVHELRRSYEDWERECTRITTDNEL
                     FDQLLARGVRDLRALDTSMDGGSVLAAGIPWYVAAFGRDALISAHQLLMVNPGCARDT
                     LELLARHQGTVDDPWRDEEPGKILHEIRKGELARSGFIPHTPYYGSVDSTPWFLIVLA
                     QYFRWTGDVAFARAMLPAAERALAWIDAYGDLDEDGLVEYQCRSPRGLTNQGWKDSWD
                     SVVHADGSLAEPPIALVEVQAYVYMAKERMAGLYEALGDGERAARLHQEASALRKRFN
                     EAFWMEDEQYFAEALDGHKRPVRSVVSNPGHALYCDIVDPELAAHVARRLLQPDMFSG
                     WGIRTMSKSAAAYNPMSYHNGSVWPHDNA"
     gene            complement(1843..>2346)
                     /locus_tag="VEO00_01425"
     CDS             complement(1843..>2346)
                     /locus_tag="VEO00_01425"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HYU56695.1"
                     /translation="LEGHPDNVAACLRGGLTVAYLSTDGWRAERVEPHPSLQPVVFVP
                     LDERLPTEDARRVLPTEVARSEATFNLSHVALAVLALTVRPGLLREALQDRLHQPARL
                     PHVPAARALFEDLREAGVPVCLSGAGPSLLAFDDGSGRIPELGPRWRVLRPGIASAGA
                     SVSASPA"
BASE COUNT          335 a          810 c          870 g          331 t
ORIGIN      
        1 cgcgttgtcg tgcggccaga ccgacccgtt gtggtagctc atcgggttgt aggcggcggc
       61 cgacttgctc atcgtccgga tcccccatcc cgagaacatg tccggctgca gcagtcgtcg
      121 agccacgtgt gcggcgagct cggggtcgac gatgtcgcag tagagcgcgt ggcccggatt
      181 cgagacgacc gagcgcaccg gccgcttgtg cccgtcgagg gcctcggcga agtactgctc
      241 gtcctccatc cagaacgcct cgttgaaacg cttgcgcagc gcgctcgcct cctggtgcag
      301 ccgcgccgca cgctctccgt cgccgagcgc ctcgtacagg ccggccatcc gctccttcgc
      361 catgtagacg taggcctgca cttcgaccag ggcgatcggc ggctcggcca ggctcccgtc
      421 ggcgtgcacc accgagtccc aggagtcctt ccagccctgg ttggtcaggc cgcgggggga
      481 ccggcactgg tactcgacca gaccgtcctc gtcgaggtcg ccgtacgcgt cgatccaggc
      541 gagcgcccgc tccgccgccg ggagcatcgc gcgcgcgaac gccacgtcgc cggtccagcg
      601 gaagtactgg gccaggacga tcaggaacca gggggtggag tccacgctcc cgtagtacgg
      661 ggtgtggggg atgaagccgg agcgggccag ctcgcccttg cggatctcgt gcaggatctt
      721 cccgggttcc tcgtcacgcc aggggtcgtc gacggttccc tggtggcggg ccagcagctc
      781 gagggtgtcg cgagcgcacc ccgggttgac catcaggagc tgatgggcgc tgatcagcgc
      841 gtcgcgcccg aaggccgcca cgtaccacgg gatccccgcg gccaggaccg accctccgtc
      901 catcgacgtg tcgagggcgc gcaggtcgcg gacgccgcgc gccaggagct ggtcgaacag
      961 ctcgttgtcg gtcgtgatcc gcgtgcactc gcgctcccag tcctcgtagg accggcgcag
     1021 ctcgtgcacg gccacgtcga actccagggc gtgtggccgc gtctggccgt tcaccggctc
     1081 caccgtgagg gagacgagcc tcgtctggtg ggcggccagt cgcagcgcga accggacgac
     1141 caccacgtcg ccgcggatct cggtctcggt ggggtcgctg ccgaactcga ccaccgagcg
     1201 ctgcaccacg ccgtccatgc cgcggtacgc gaactccacg atccgcccgt cggcgcgcgg
     1261 aggctcgtag tggccgcgca cgtcgcgcgc cagcccgcgc acctcgaaga cgtcggcgaa
     1321 gtcggcaccc agcgagaact cgagctgcac ggagaccggg taggggttgt agttcttcac
     1381 ccggatacgc tcgaacagcc gcccgttgat cgtgcggatc cgacggacgt tcagcgtctg
     1441 ctgcgggacg gccagctgat cgccctcgaa caggtctggg ttcgtcaggt cgacgtagga
     1501 catgtaggcc cgctcggccg atgaggacag gaggacgggt tcccgtccgg agaggcgcat
     1561 caggaagcgc gacaggaagc gcgtgtcctg gtggtagaag ccgaggccca gctcgcgccg
     1621 gtcgtcgagg ttgccctccg tgtcggaata caggaaggtc tcgccttcct tcgtcgccag
     1681 gaccttgctg gcgaggtcgg tgacgaggac ggtggtggcg gtctcgcgga tcccagggaa
     1741 ctcgggcccg ccggcgggct ggggctctcg ggccggctcg ctcacccggt cagcataggg
     1801 tggccgctcg gcgcattcca cgtccgggcg gcgaaaccac tcctacgccg gggaggcgga
     1861 gaccgaggcg ccggccgagg cgatccccgg tcgcaggacg cgccagcggg gcccgagctc
     1921 ggggatgcgg ccggacccgt cgtcgaaggc cagcagcgac gggccggcgc cgctcaggca
     1981 gacgggtacg ccggcctcgc gcaggtcctc gaagagggcg cgcgcggcgg ggacgtgcgg
     2041 caggcgggcc ggctggtgga ggcggtcctg gagcgcctcg cgcaagaggc ccggccgcac
     2101 ggtgagcgcc aggacggcca gcgccacgtg cgacaggttg aacgtggcct cggagcgcgc
     2161 gacctcggtc gggagcacgc gccgggcgtc ctccgtgggc aggcgctcgt cgagcgggac
     2221 gaagaccacc ggctgcagcg acgggtgcgg ctccactcgc tccgcgcgcc agccgtcggt
     2281 ggagaggtag gcgaccgtca ggccgccgcg caggcaggcc gcgacgttgt cggggtggcc
     2341 ctcgag
//
LOCUS       DASPWO010000060         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_688814, whole genome shotgun sequence.
ACCESSION   DASPWO010000060 DASPWO010000000
VERSION     DASPWO010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688814"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VEO00_01430"
     CDS             complement(<1..376)
                     /locus_tag="VEO00_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56696.1"
                     /translation="MRSTMVVAALALALGAGGCGRGGTTAGSPSTGQGDASPLPQGSE
                     TVRLDPSDFVDGIDNPYWPMVPGSRWVYRETDADGNVQRVEVTVTGRAKEILGIAATV
                     VHDVVSEDGRLVEDTLDWYAQDR"
     gene            508..1185
                     /locus_tag="VEO00_01435"
     CDS             508..1185
                     /locus_tag="VEO00_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU56697.1"
                     /translation="MRVLVVEDEVKMASLLSRGLKEEGYAVDVAATGQEGVWAATENP
                     YDAIVLDVMLPDVDGFEVCRRLRETGRWAAVIMLTARDGVAHRVRGLDAGADDYLTKP
                     FSFAELLARLRALLRRDAGERPVLLRAGDLTLDPATRRVTRGDAPVELTAKEFALLEY
                     LLRHPDEVLSRTRLIEHVWDFAYEGDSNVVDVYVRYLREKIDRPFGRHSIETVRGAGY
                     RLRADGG"
     gene            1188..>2219
                     /locus_tag="VEO00_01440"
     CDS             1188..>2219
                     /locus_tag="VEO00_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYU56698.1"
                     /translation="MPIRVRLTLVVVALTAVVLAGLGLFVFAETRQSLQATVDRGLRS
                     QAQAIVTAVQAGDLSLPDPGALVEPEEAFAQLLRRDGRVLESTPGMSPAPLLSASEAR
                     DLAGPRASSADVRGPEEILPARLLAVPATTDLVVVAGTSVEDELEVLARLRTQLSVGG
                     LGALLASAAIGWIVSGAALRPVERMRREAERISTTDLERRLPQPATRDELARLGTTLN
                     AMLERLRAGVERERRLVDDASHELRTPLAILKTELELALRRARTPEELQGALSSASEE
                     TERLSRLAEDLLVLARADRGRLPIHRESVDVRAAIGAAVEPFRAAAAARRVALEVDAA
                     GAGTAALDPL"
BASE COUNT          263 a          826 c          817 g          313 t
ORIGIN      
        1 cccggtcctg ggcgtaccag tcgagcgtgt cctccacgag ccgcccgtcc tcgctcacca
       61 cgtcgtggac caccgtcgcg gcgatcccca ggatctcctt cgcccggccg gtcacggtga
      121 cctcgacgcg ctgcacgttg ccgtcggcgt cggtctcccg gtacacccag cggctgcccg
      181 ggaccatcgg ccagtacggg ttgtcgatcc cgtcgacgaa gtcggacggg tcgaggcgga
      241 cggtctcgct cccctgcggg agcgggctcg catccccctg cccggtggac ggggaaccgg
      301 ccgtcgtccc accacggccg catccaccgg ccccgagcgc caaagccagg gcggcgacca
      361 ccatggtgct gcgcatcgtt cctccctctc ctccggttgt cgcggtcacg atgcgcccgc
      421 gcccatgaga ggacggtgag cggccgatgg ggagccgccg gccctggttc tcatcctcgt
      481 ctcatcgtgc cgacatatgc tcacgtcgtg cgggtcttgg tggtggagga cgaggtgaag
      541 atggcgtcgc tcctctcgcg cgggctgaag gaggagggct acgccgtcga tgtcgcggcc
      601 accggccagg agggcgtctg ggccgccacc gagaacccct acgacgcgat cgtcctcgac
      661 gtgatgctcc ccgacgtgga cgggttcgag gtgtgccgcc ggctccggga gaccgggcgc
      721 tgggcggcgg tgatcatgct cacggcccgc gacggcgtgg cccaccgtgt ccgcgggctg
      781 gatgccggcg ccgacgatta cctgacgaag ccgttctcgt tcgccgagct gctggcgcgc
      841 ctgcgggcgc tgctccggcg cgacgccggc gaacggccgg tgctgctccg cgccggcgat
      901 ctcacgctcg acccggccac ccgccgcgtc acccgcggcg acgcgcccgt ggagctgacg
      961 gcgaaggagt tcgcgctgct cgagtacctc ctgcggcacc cggacgaggt gctgtcgcgg
     1021 acgcgactga tcgagcacgt ctgggacttc gcctacgagg gcgactcgaa cgtggtggac
     1081 gtctacgtgc gctacctgcg cgagaagatc gaccgcccct tcggccgcca ctccatcgag
     1141 acggtccgcg gcgcgggcta ccgtctgcgc gcggacggag ggtaacggtg ccgatccggg
     1201 tccggttgac gctcgtcgtc gtcgccctga cggcggtggt gctcgccggg ctcggcctgt
     1261 tcgtgttcgc cgagacccgg cagagcctgc aggcgacggt cgaccggggc ctgcgatctc
     1321 aagcccaggc gatcgtcacg gccgtgcagg ccggcgacct gtcgctcccc gatcccggcg
     1381 cgctcgtcga gcccgaggag gcgttcgcgc aactgctccg ccgcgacggc cgggtgctgg
     1441 aatccacgcc ggggatgtcg ccggcaccac tgctgtcggc gagcgaggcc cgtgacctcg
     1501 cggggccgcg cgcctcctcc gcggacgtcc gcggcccgga ggagatcctc ccggcgcgcc
     1561 tcctcgcggt gccggcgacg accgatctgg tggtggtggc cgggacctcg gtggaggacg
     1621 agctggaggt gctcgcccgt ctgcggacgc agctctccgt cggcgggctc ggcgcgctcc
     1681 tggcctcggc ggcgatcggg tggatcgtct ccggcgcggc ccttcgcccg gtcgagcgga
     1741 tgcgccgcga ggccgagcgc atctccacca cggacctcga gcgtcgcctg ccgcagccgg
     1801 cgacccgcga cgagctggcc cggctgggga cgacgctgaa cgccatgctc gagcgcctcc
     1861 gggccggtgt cgagcgcgag cggcgcctgg tcgacgacgc cagccacgag ctgcgcaccc
     1921 ccctcgccat cctgaagacc gagctcgagc tggcactgcg acgggcccgg acccccgagg
     1981 agctgcaggg cgccctctcc agcgcgagcg aggagaccga gcgcctgagc cgcctggccg
     2041 aggacctgct ggtcctggca cgggccgacc gcggccgcct gccgatccac cgggagtctg
     2101 tggatgttcg cgcggcgatc ggggcggcgg tcgagccgtt ccgggcggcg gcggccgcgc
     2161 ggcgcgtggc cctggaggtg gacgccgccg gcgccggaac cgccgcgctc gacccgctg
//
LOCUS       DASPWO010000061         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1031641, whole genome shotgun sequence.
ACCESSION   DASPWO010000061 DASPWO010000000
VERSION     DASPWO010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031641"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VEO00_01445"
     CDS             <1..917
                     /locus_tag="VEO00_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56699.1"
                     /translation="GDDPVEGATITLGLDDDAVCTITNDDQPGTIVVRKITKPLNAST
                     SFTFQTTGTGYAGFSLGSGGTNSQTLDAGSYTVKELVPLGWVLTGLGGSTDPTTPYSC
                     TVTGGGGSTGVGDLNTQTATISLKNGDTVTCVFENTGQGVTRTQGFWATHTPLANIAW
                     FGGTAFGHTFPGVANVSGIGDELICGRPVDTLGKLMGAFWSDVSKKSTGAKRTALDQA
                     RMQLLQQLIAAELNASAFGSVPSVGSFAAWETALCGTNTNAIKTAQQQAASFNTQGDS
                     GTFTPGTSADSKYARSIANIPFWDIIKP"
     gene            1196..2623
                     /locus_tag="VEO00_01450"
     CDS             1196..2623
                     /locus_tag="VEO00_01450"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56700.1"
                     /translation="MSGIGVSAIDRARLGLVLGGSALTLAWILVAVVIRVVRQPRAAP
                     TGPPTLEPGPEPPAVSNLLTHNFRPTRDALAATVIDLAARGVVEIEKRPPHGYACRLR
                     SAATEDLAPWERRVLGVLEEKAVNGIVPTAALTTGTAASSRRWWKGFVKDVQEETKAR
                     GLSRDIWDAGAQRTVLVLALLPLPLFALAAFNLAAIPGYLVPAAVVYGTLVNDRQRDT
                     TSGLERASVWLGFRAKLRENPSFPALPPTAVAIWERILAHGAAVGVAEAAVRAIPMGP
                     EDDRRAWSAYGGEWREVRVRYGRRPGWGASPAGAIFAGVLALAVCAGIGFGLLTLWRF
                     LTDQGLGLGLEAAGRQGLEVTGVERIVGWALLGIGAVLALIAARSLLVLVSGVIDAVT
                     PDRVVTGELIRLRMRGSEESRTYHGAVYDGSATRVRAYLLEPLQFGALAQGQVVRAVV
                     TRTLGHVRAIDPVPQPAGAPGPLVG"
BASE COUNT          385 a          992 c         1022 g          376 t
ORIGIN      
        1 acggcgatga cccggtggag ggcgccacga tcacgctcgg tctcgacgac gacgcggtct
       61 gcaccatcac caacgacgac cagcccggca cgatcgtcgt ccggaagatc accaagcccc
      121 tgaacgcatc gacgagcttc accttccaga cgacgggtac gggatacgcc ggcttctcgc
      181 tgggcagcgg aggaacgaac agtcagaccc tggacgccgg gagctacacc gtgaaggagc
      241 tcgtgccgct ggggtgggtg ctcaccgggc tcggcggttc caccgatccc accacgccct
      301 acagctgcac ggtcaccggc ggcggcggaa gcacgggcgt cggggacctg aacacgcaga
      361 ccgcgaccat cagcctgaag aacggcgaca ccgtgacctg cgtgttcgag aacaccggcc
      421 agggcgtgac ccggacccag ggcttctggg ccactcacac cccgctcgcc aacatcgcct
      481 ggttcggagg aaccgcgttc gggcacacct tccccggcgt ggcgaacgtg tccggcatcg
      541 gcgacgagct catctgtggc cggcccgtgg acacgctcgg gaagctgatg ggcgccttct
      601 ggagcgatgt ctcgaagaag tccacgggtg ccaagcggac ggcgctcgac caggcgagga
      661 tgcagctcct gcagcagctg atcgcggccg agctgaacgc ctccgccttc ggcagcgtgc
      721 cgagcgtcgg aagcttcgcg gcgtgggaga cggcgctctg tggaaccaac acgaacgcca
      781 tcaagacggc gcagcagcag gcggcgtcct tcaacaccca gggggatagc ggcaccttca
      841 ccccggggac gtccgcggac agcaagtacg ctcgttcgat cgcgaacatc ccgttctggg
      901 acatcatcaa gccgtgagga gtcagcgcac ccgcggctgc tgaggagcgg ctggacggag
      961 ggcccggcat cgccgggccc tccgccatgt cccggggtga gcgagacgca tccgtctcga
     1021 tcggcagctc agccctccgc gcgaacccga cctacgcgag ccgacggatc gattcctgcc
     1081 cacccttcca tccgtacgcg tagagcacgg catcgcagcg gcggccccga ccgatcgtgg
     1141 gcccgcgggt gacctcggtt gacggcctcg atcgaccggg gaagcatggc ggccggtgtc
     1201 cggcatcggg gtctcggcga tcgatcgagc caggctcggc ctggtgctcg gaggctcggc
     1261 gctgacgctc gcctggatcc tggtcgcggt ggtcatccgt gtggtgcggc agccgcgagc
     1321 ggccccgacc ggcccgccga ccctcgagcc ggggccggag ccgccggccg tctcgaacct
     1381 gctgacccac aacttccggc cgacccgtga cgccctggcg gcgacggtga tcgacctcgc
     1441 ggcccgcggc gtggtcgaga tcgagaagcg cccgccgcac gggtacgcct gtcggctccg
     1501 ctcggcggcc acggaggacc tggcgccgtg ggagcgccgc gtgctcgggg ttctcgagga
     1561 gaaggccgtg aacggcatcg tgcccaccgc ggcgctgacc accgggaccg cggcgtcctc
     1621 ccgccgctgg tggaagggat tcgtcaagga cgtgcaggag gagaccaagg cgcgcgggct
     1681 gtcgcgcgac atctgggacg ccggcgcgca gcggaccgtg ctcgtcctgg cgctgctgcc
     1741 gctgccgctg ttcgcgctcg cggcgttcaa cctcgccgcg atccccggct acctggtccc
     1801 ggccgcggtc gtctacggga cgctggtcaa cgaccgccag cgcgacacga cgtccgggct
     1861 ggagcgcgcc tcggtctggc tcggcttccg ggcgaagctc cgcgagaacc cgagcttccc
     1921 ggcgctgccg cccaccgccg tggcgatctg ggagcggatc ctggcgcacg gcgcggcggt
     1981 cggggtggcc gaggccgccg tccgggccat cccgatggga ccggaggacg accgccgggc
     2041 ctggagcgcc tacggcgggg agtggcgcga ggtccgggtg cgctacggtc gccgcccggg
     2101 ctggggtgcg agccccgccg gggcgatctt cgccggcgtg ctggcgctgg ccgtctgcgc
     2161 cgggatcggg ttcgggctgc tgacgctgtg gcgcttcctg accgaccagg gcctcggcct
     2221 cgggctggag gccgccggcc ggcagggcct ggaggtgacc ggcgtggaac gcatcgtggg
     2281 ctgggcgctg ctcgggatcg gcgccgtcct ggcgctgatc gccgcgcgct cgctgctggt
     2341 gctcgtctcg ggcgtgatcg acgcagtcac gccggaccgg gtggtgaccg gcgagctgat
     2401 ccgcctgcgg atgcgcgggt ccgaggagag ccgcacgtac cacggagcgg tctacgacgg
     2461 ctcggcgacg cgggtccgcg cctacctcct ggagccgctc cagttcggcg ccctggccca
     2521 ggggcaggtg gtgcgggccg tcgtcaccag gaccctcggc cacgtccgcg ccatcgaccc
     2581 ggtgccgcag ccggccgggg cgccggggcc gctcgtcggc tagtcggcgc gagtgccgac
     2641 gcggttgcgc aggacgccga tgccctccgc ctcgagctcg acctgctgca ccaggcggcc
     2701 ttcccggcga agccggccgg agaggcctgg acgtcgtacg cgttcttcgg gatcgccctg
     2761 ctcggcgcgg cgatg
//
LOCUS       DASPWO010000062         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_180914, whole genome shotgun sequence.
ACCESSION   DASPWO010000062 DASPWO010000000
VERSION     DASPWO010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180914"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2019
                     /locus_tag="VEO00_01455"
     CDS             <1..>2019
                     /locus_tag="VEO00_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HYU56701.1"
                     /translation="ERATVFVPDGAVKHFIARFDQYATERTAKKGEPRHRDMVDPIAA
                     LRLATLRTLWTDAPEAYPRAGEAIWWEVWLRRQDGRELQRLVEFAGLAGIQLRDRRLE
                     FHDRIVTLAFSSAERLSDSLDVLNDVAELRRAKESAGVFAGMGPREEAEWLRDLEERT
                     TPAPDDATAVCVLDTGINRAHPLLEPSLAAEDTHACDPGWGTHDHDGHGTEMGGLALY
                     GDLGPLLAGQQPVRLTHVLESVKILPPPTAPANRPELYGAVTAEAASRVEVQAPDRRR
                     AFSMAITAIDERDQGQPTSWSAAIDALAAGRSFDPASQGLVYLDDNDPAQRLFVVCAG
                     NVTNPDVNHLDRSDTEPIHDPGQAWNALTVGAHTEAAWPDEDPEWEGWKPVARPGELS
                     PWSTTSVPFADAWPIKPDVVFEGGNVVHDGAGHVNFPVPALSVLSTHYRPAEKLFVLS
                     WATSASTAQVARIAGAITADYADYWPETVRALIVHSAEWTPAMRAHLSGSGSAKRARL
                     RLTRRYGFGVPNLERALRSANDVLTLVAQATLSPFDRGRMREMHLHPLPWPKEALEGL
                     AETPVRLRVTLSYFVEPNPARRGWKRRHRYASHGLRFEVKTPTEDLDNFRKRLNQRAL
                     EEDEERPDTTGDSAAWFLGEQARNRGSVHSDIWVGTAADLAERGYVGV"
BASE COUNT          341 a          728 c          670 g          280 t
ORIGIN      
        1 ccgaacgggc caccgttttc gtccccgacg gcgccgtcaa gcacttcatc gcacggttcg
       61 atcagtacgc cacggagcgg accgcgaaga agggcgagcc gcgccacaga gacatggtgg
      121 atccgatcgc ggccctccgc cttgcgaccc tccgcaccct ctggaccgac gcacccgagg
      181 cgtatccccg ggcgggcgag gcgatctggt gggaggtctg gctgcggcgg caggacggcc
      241 gggagctgca gcggctcgtc gagttcgcgg gactcgctgg gatccagctc agggaccgaa
      301 ggctggagtt ccacgaccgc atcgtcacgc tcgccttctc gagcgcggaa cgtctctccg
      361 actccctcga cgtgctcaac gacgtggccg agctgcgtcg tgccaaggag tccgccgggg
      421 tcttcgccgg catgggtccg cgcgaagagg ccgaatggct cagggacctc gaggagagga
      481 cgactcccgc tcctgacgac gcaacggccg tctgcgtcct cgacaccggc atcaaccggg
      541 cccatccact gctcgagccc tcgctggccg ccgaagacac ccacgcctgc gatccaggct
      601 ggggaaccca cgaccacgac ggccacggaa cggagatggg gggactggcc ctctacggcg
      661 acctgggtcc cctgctggcc ggacagcaac cggttcgcct gacgcacgtc ctcgaatccg
      721 tgaagatcct ccctcccccg accgcgccgg cgaaccgtcc cgagctctat ggcgccgtga
      781 ccgctgaagc ggcgagccgg gtcgaggtcc aggcccccga ccggaggcgt gccttctcga
      841 tggcgatcac tgccattgac gagcgcgacc aggggcagcc cacgtcgtgg tcggcggcga
      901 tcgacgcgct cgccgccggt cggtcgttcg atccagcgtc ccaggggctc gtctacctgg
      961 acgacaacga cccggcacag cgtctctttg tcgtgtgcgc cggcaacgtg acgaaccccg
     1021 acgtgaacca cctcgaccga agcgacactg agcccatcca cgatccgggg caagcctgga
     1081 atgccctcac cgtcggcgcc cacacggaag cggcatggcc agacgaggac ccggagtggg
     1141 aagggtggaa gccggtcgct cgcccagggg agctgtcgcc ctggagcacg acctcggtgc
     1201 ccttcgcgga tgcgtggccg atcaagccgg acgtggtgtt cgagggcggc aacgtcgtgc
     1261 acgacggcgc cggccacgtc aacttccccg tgccggcgct cagcgtcctg tccacgcact
     1321 accggccagc cgagaagctc ttcgtgctct cctgggcaac gagcgcgtcc accgcccagg
     1381 tcgcacggat cgcgggggcc ataacggcgg actacgcgga ctattggccg gagacggtgc
     1441 gcgcgctcat cgtgcactcg gccgagtgga cacccgccat gagggcccat ctcagcggat
     1501 ccgggagcgc gaagagggcc cgcttgcgcc tcacccgccg ttatggattc ggtgtgccca
     1561 acctcgagcg ggcacttcgc agcgccaacg atgtcttgac gctcgtagcg caggcgaccc
     1621 tctccccttt cgacaggggc aggatgaggg agatgcacct gcaccccctg ccctggccga
     1681 aggaggccct ggagggcctc gcagagacgc cggtacgcct ccgagtcacc ttgtcctact
     1741 tcgtcgagcc gaaccccgca cgacggggct ggaaacgccg ccaccgatac gcatcgcacg
     1801 gcctccgctt cgaagtcaag acgccgaccg aagacctgga caatttccgc aagcgcctca
     1861 accagcgcgc gctcgaggag gacgaggagc gccccgacac caccggcgac tcggccgctt
     1921 ggttcctggg cgagcaggcc cgcaacaggg gctccgtgca ctcggacatc tgggtgggca
     1981 cagccgccga cctggcggag cgaggatatg tcggcgtct
//
LOCUS       DASPWO010000063         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_777956, whole genome shotgun sequence.
ACCESSION   DASPWO010000063 DASPWO010000000
VERSION     DASPWO010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777956"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="VEO00_01460"
     CDS             complement(<1..1460)
                     /locus_tag="VEO00_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56702.1"
                     /translation="MSQVTVGTVPVRLPRRTLVREQMLVTGAQLVAGAGNVVFALVMA
                     RVLAPAAFTDLAAFLVCYLLLHLPAAGLGAGGALAGAGAGALRRRALGAGIAGGLVLA
                     AVSPALASLLRLPLALPLLLAAAAPAAAALALERGRLYGTGRHPQAALTLVLEPVSRL
                     VAGIALARVAGAPGAAAGVVVGGYLALLATRTAGQAPVVTVPARTGAAVAAFFLLAVV
                     QNQDLLLAARVLPEVDAARFAALSTLGGVAAFATATIPVVLLPRASDQPHALRVALAV
                     AAALGGAAVLAFTVAAGPLTDLVFGGRYGAIAPLAAPYVLGMALLGVTRVLAADRIAR
                     GTGTTVVVATAIAAIVQAGLIVRFGTSVAAVAAVTVATTAGLAATLAVATVVRLPAPS
                     RTVADVASWLRERAVLAVTAAAAAGLALRLLVTRGLWVDEAISVKQAGLPFGTMLDRL
                     AAGDVHPPLHASLLWVTVRVFGDGEFAVRLPSLVAGV"
     gene            complement(1457..2575)
                     /locus_tag="VEO00_01465"
     CDS             complement(1457..2575)
                     /locus_tag="VEO00_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HYU56703.1"
                     /translation="MPDVLLAGAFGQGNPGDEALLTAFLRALPPGRVAVTSRDPRATE
                     ARHCCDAFAPNGRDAMRELRRADAVVIGGGSVFKTLHPSTGRAPHSLLANTTLVARGA
                     RALGKPLLLIGVGVGNLTSHRSQRLSRKLVRAADVLVLRDEESADVLTGIGAPAPFRV
                     GADPAWTLLDDPPVRNQHEPGTTAVVALSHLAGGLELPRLVAEWIRVLQAAGLHVVLQ
                     PWQANGRGDDRRLARQVAARVGDVDVVDPPADLVDAREQIAGAGLVLGMRFHALMAAA
                     GAGVRFVAVDHELKLGALARRFSQPTVAPTESRDRMAGAVADALAGPAPGVSELLEQV
                     ALAEEGFRLLRLVVEGGAPDPDELRGLPLEPAPWEVPQ"
     gene            complement(2535..>3312)
                     /locus_tag="VEO00_01470"
     CDS             complement(2535..>3312)
                     /locus_tag="VEO00_01470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU56704.1"
                     /translation="ELHRVRAPARLARLGLAGVQRAVRRFSDAVIVHEPAFARHVRGA
                     RVVPHGVETPPLPDREAARISLGLDHRLTVLCFGFLAPYKGLETSLEAAGLAGPAVQL
                     VIAGGEHPRLAGRDPYASELQAKGEGLARFTGLVPDDDVPTWFAAADLALFLYPRPFS
                     SSGALALALAYRTPVLMSRELGDCTGSPAAVRVESDPRRVAERLRALASDPAELERLR
                     GAVGRLREERSWPRVAHRHLDVYEEVRRARCPAGRRLRPR"
BASE COUNT          474 a         1224 c         1308 g          306 t
ORIGIN      
        1 acgccggcga cgagtgaggg cagccgtacc gcgaactcgc cgtcgccgaa gacccggacc
       61 gtcacccaca gcaaggacgc gtgcaacgga gggtggacgt cgccggcggc gaggcgatcg
      121 agcatggtcc cgaacggcag ccccgcctgc ttcacgctga tggcctcgtc cacccagagc
      181 ccgcgcgtga cgagcagacg cagggcgagc ccggcggcgg cggccgcggt gactgcgagc
      241 accgcccgct cgcgcaacca ggacgccacg tcggcgacgg tgcggctcgg ggcggggagg
      301 cgcacgacgg tcgcgaccgc cagggtcgcg gcgagcccgg cggtggttgc gaccgtgacc
      361 gcagccaccg cggcgacgct cgtcccgaac cgcacgatga ggccggcttg gacgatggcg
      421 gcgatcgcgg tcgcgacgac gacggtcgtg cccgtcccgc gggcgatgcg gtcggccgcc
      481 agcacccgcg tgacgccgag caacgccatg ccgaggacgt acggggccgc cagtggcgcg
      541 atcgcgccgt accggccgcc gaagacgagg tcggtgagcg ggccggccgc gaccgtgaac
      601 gccagcaccg cggccccgcc gagggcggcg gcgacggcca gcgcgacacg gagtgcgtgg
      661 ggctggtcgc tggcgcgcgg caggaggacg accgggatcg tggcggtggc gaacgcggcc
      721 acgccgccca gcgtcgagag ggcggcgaag cgagccgcgt cgacttcggg gagcacgcgt
      781 gccgcgagga gcaggtcctg gttctgcacc accgcgagca ggaagaacgc ggccactgcg
      841 gcaccggtgc gggccggaac ggtgacgacg ggcgcctggc cggcggtgcg ggtcgcgagc
      901 agggcgaggt agccgcccac aaccactccg gcggcggcgc cgggcgcgcc ggcgacgcgc
      961 gcgagcgcga tgccggcgac gagccggctc accggctcga gcaccagagt gagcgcggcc
     1021 tgcggatggc ggccggtccc gtacaaccgg ccccgctcga gcgccagcgc ggcggccgcc
     1081 ggcgcggcgg ccgccaggag cagaggcaac gcgagcggga gccggagcag cgatgcgagc
     1141 gcgggggaga cggccgccag caccaagccg ccggcgatgc ccgcgccgag cgcgcgccgc
     1201 cgcaacgccc ccgccccggc gccggcgagc gcgccgccgg cgccgagccc ggcggcgggc
     1261 aggtgcagga ggaggtaaca gaccaggaac gcggccaggt cggtgaaggc ggccggggcg
     1321 aggacccggg ccatcacgag cgcgaagacg acgttgccgg cgccggcgac gagctgcgcc
     1381 cccgtcacca gcatctgctc gcgcacgagc gtccggcgcg ggagccggac gggcacggtg
     1441 ccgaccgtca cctggctcac tgcggcacct cccacggcgc cggctcgagg ggcagtcccc
     1501 gcagctcgtc gggatcgggc gcgccgccct cgacgacgag gcgcaggagg cggaagccct
     1561 cctcggccag cgcgacctgc tccagcagtt ccgacacgcc cggcgccggc cccgccagcg
     1621 cgtcggccac cgcgcccgcc atccgatcgc gtgattcggt cggggcgacg gtcggctgcg
     1681 agaaccggcg cgccagcgcg ccgagcttga gctcgtggtc gacggcgacg aaccgcaccc
     1741 ccgctcccgc cgccgccatc agtgcgtgga accgcatccc gaggacgagc cccgcgccgg
     1801 cgatctgttc gcgcgcgtcg acgagatcgg cgggtgggtc caccacgtcg acgtcgccca
     1861 cccgcgccgc tacctgccgg gcaaggcggc ggtcgtcgcc gcggccattc gcctgccacg
     1921 gctggaggac gacgtgcagt cccgcggcct ggaggacgcg gatccattcg gcgaccaacc
     1981 gcggcagctc gagcccaccg gcgagatggc tgagcgcgac gaccgcggtg gtcccgggct
     2041 cgtgctggtt ccgcactggt ggatcgtcga gcagcgtcca cgccgggtcg gcgccgacgc
     2101 ggaacggggc cggcgcgccg atacccgtga gaacgtcggc cgactcctcg tcccggagca
     2161 cgagcacgtc ggcggcccgt acgagcttgc gactcagccg ctgcgaccgg tggctggtca
     2221 ggtttcccac ccctacaccg atcagcagca agggcttccc gagggcgcgg gcgccgcgcg
     2281 cgacgagcgt cgtattcgcc aacagcgagt ggggcgcccg gcccgtcgat ggatgcagcg
     2341 tcttgaacac gctcccgccc ccgatcacga ccgcgtcggc gcggcggagc tcgcgcatcg
     2401 catcgcgacc attgggcgcg aacgcgtcac agcagtggcg ggcttcggtc gcgcgcgggt
     2461 cgcggctggt gaccgcgacc cgacccgggg ggagggcgcg caggaacgcg gtgaggagcg
     2521 cctcgtcgcc ggggttacct tggccgaagg cgccggccag caggacatcg ggcacgcctc
     2581 acctcctcgt acacgtcgag gtggcggtgc gcgacgcgcg gccacgagcg ctcctcccgc
     2641 aggcggccca ccgccccgcg gagccgttcg agctcggcgg ggtcggacgc gagcgcgcgc
     2701 aggcgctcgg cgacccgccg cgggtccgac tcgacccgca ccgcggcggg cgagccggtg
     2761 cagtccccga gctcgcgaga catgagcacc ggagtgcggt acgcgagcgc gagggccagt
     2821 gccccgctgc tggagaacgg gcgcgggtag aggaacagcg cgaggtcggc ggccgcgaac
     2881 caggtgggca cgtcgtcgtc gggcacgaga ccggtgaagc gggccagccc ctcgcccttg
     2941 gcctgcagct ccgatgcata cgggtcgcgt ccggcgaggc gggggtgctc gccgccggcg
     3001 atcacgagct ggacggccgg cccggccagg ccggcagcct cgagcgacgt ctcgagcccc
     3061 ttgtagggcg cgaggaaccc gaagcacagc accgtcaggc ggtggtcgag cccgagcgag
     3121 atgcgggcgg cctcccggtc gggcagtggc ggcgtctcga cgccgtgggg aaccacccgg
     3181 gcgccgcgca cgtggcgggc gaacgcgggc tcgtgcacga tgacggcgtc cgagaagcgg
     3241 cgcaccgccc gctggacgcc cgccaacccg agacgggcaa gccgggcggg ggcccggacc
     3301 cggtggagct cg
//
LOCUS       DASPWO010000064         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_432472, whole genome shotgun sequence.
ACCESSION   DASPWO010000064 DASPWO010000000
VERSION     DASPWO010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432472"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1513
                     /locus_tag="VEO00_01475"
     CDS             71..1513
                     /locus_tag="VEO00_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HYU56705.1"
                     /translation="MLAAALLACGETAGPGLASPRIDYVDGALAPILVRRQAVVIEGL
                     GFGATQGAGTVTFARTGGGEVAAPVAAPADWSDLAIRTAVPDAAAAGAGTLAVTTAAG
                     RRLTATVHVLPHVVFDTAALAWRSRGSFPQAPVGVALAALEIPSGNGLATTLYAAGGA
                     EQASGALVPDSGVYLARAQPEGAVGAWVRQRDATDFTSRVLPLPRAFAAAAIATRYNS
                     RFPGAALYVIGGVGSAGRAQASVLAADITADSVVARFTPLEQLPAPLAGAIAVVRRGR
                     IYVIGGTDSLGRPQQDVFVGRIGLDGHIDGWYVQPSLPAPRAYGGGVVLDARAVAFGG
                     LADSVPPGGGLDPALPRLASGDTAAVSLLSGFFTGAWAPETALLPAGRSQFATLDLGD
                     VILLVGGMYAGAETNSAETIAAAVAGNSLGPFAGPVGTSRIADPPNNGGILVGPSGVT
                     WREADGSRRGLVLGGIDLSTGQRKAGVWGF"
     gene            1567..>2568
                     /locus_tag="VEO00_01480"
     CDS             1567..>2568
                     /locus_tag="VEO00_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HYU56706.1"
                     /translation="MRTLVIVGLVLTGGASGLAAQHAHQFELGAFGSFTRYDRVFNLD
                     NQAGGGARLGYFFGDVVSAEIEGGYQSPSPKSGTASPTLAIGSASLVLNFGSENNLFY
                     ILGGYSRLDFEKNPSYLFTDNAVHGALGDRVFLGGGAALRFEVRAIYAPNTGFAAGDW
                     AGHVVGSVGLSLFAGGGRPPDADRDGVPDKRDVCPNTPTSARVDATGCPLDSDRDGVP
                     DGLDQCAGTPTGATVNAAGCPADSDSDGVYDGLDQCVNTPAGAHVDAKGCPADADGDH
                     VADGIDQCPNTPAGATVDAAGCPLDSDKDGVPDGLDKCPNTSPGMEVDDVGCQRFRDA
                     "
BASE COUNT          322 a          935 c          956 g          355 t
ORIGIN      
        1 ctcgccgaca gtctcgccag ggccgtgcgg ttcgtgcggg gtgagaacgg ccggcagtag
       61 gctcgcgccc gtgctcgccg ccgcgttgct agcgtgcggc gaaacggccg ggccggggct
      121 cgcgtcgccg aggatcgact acgtcgacgg cgcgctcgcg ccgatcctcg tgcgccgcca
      181 ggccgtggtg atcgagggcc tgggcttcgg cgcgacccag ggcgccggca ccgtcacgtt
      241 cgctcgcacc ggtggcggcg aggtggcggc gcccgtcgcc gcgcccgccg attggagcga
      301 tctcgccatc cgcaccgcgg tcccggacgc cgcggcggcg ggagcgggga cgctcgccgt
      361 caccaccgcc gccgggcggc ggctcaccgc gaccgtccac gtcctgccgc acgtcgtgtt
      421 cgacaccgcc gcgctggcct ggaggtcgcg tggctctttc ccacaggcgc cggtcggtgt
      481 cgcgctggcg gcgttggaga tcccctccgg gaacggcctc gcgacgacgc tatatgcggc
      541 gggcggcgcc gagcaggcaa gcggcgcgct cgtgccggac agcggcgtgt acctggcgcg
      601 cgctcagccg gagggagcgg tcggcgcctg ggtgcgccag cgcgacgcga ccgacttcac
      661 ctcgcgcgtc ctccccctcc cgcgggcgtt cgcggccgcc gcgatcgcca cacggtacaa
      721 ctcgcggttc ccgggcgcgg cgctgtacgt gatcgggggc gtcggttccg ctgggcgggc
      781 gcaggcctcc gtcctggccg ccgacatcac cgccgacagc gtcgtggcgc gcttcacgcc
      841 cctcgagcag ctgcccgcgc cgctcgccgg cgcaatcgcg gtcgtacggc ggggccgcat
      901 ttacgtgatc gggggcacgg actccctcgg gcgtccgcag caggatgtgt tcgtggggcg
      961 gatcgggctc gatgggcaca tcgacggctg gtacgtccag ccgtcgcttc ccgcgccgcg
     1021 cgcgtacggc ggcggtgtcg tgctcgacgc ccgcgcggtg gcgttcggcg gcctggccga
     1081 cagcgtcccg ccgggcggcg ggctcgatcc cgcgctgccg cggctcgcgt cgggcgacac
     1141 cgccgccgtg agcctcctct cggggttctt cacgggcgcg tgggcgccgg agacggcgct
     1201 gctccccgcg ggccggtcgc agttcgcgac gctcgatctc ggcgacgtca tcctgctcgt
     1261 gggcgggatg tacgccggcg ccgagacgaa cagcgccgaa accatcgccg cggccgtggc
     1321 aggcaactcg ctcggtccgt tcgccggacc ggtggggacg agcaggatcg ccgacccgcc
     1381 gaacaacggc ggtatcctcg tcgggccgag cggcgtcacg tggcgcgagg ccgacggatc
     1441 gcgtcgcggg ctggtactcg gcggcatcga cttgagcacg ggacagagga aagcgggcgt
     1501 ctggggtttc taatgggcga ccgcgttatt accgtagtgc cacgccctcg ggggaggagt
     1561 cgctccatgc ggacgctcgt catcgtggga ctggtgctga ccggtggggc ctcgggtctc
     1621 gccgcgcagc acgcacacca gttcgagctc ggagccttcg gatcgttcac ccggtacgac
     1681 cgggtcttca acctcgacaa ccaggccggc ggcggcgctc gcctcggcta cttcttcggc
     1741 gatgtcgtca gcgcggagat cgagggcggc taccagagcc cgagcccgaa atcgggcacc
     1801 gcatccccca ccctggcgat cggcagcgcg agcctggtgc tcaacttcgg gagcgagaac
     1861 aacctcttct acatcctcgg cggctactcg cggctcgact tcgagaagaa cccgtcgtac
     1921 ctcttcaccg acaacgccgt gcacggggcg ctgggcgacc gcgtgttcct cgggggcggc
     1981 gccgcgctgc gcttcgaggt gcgcgccatc tacgctccca acaccgggtt cgcggccgga
     2041 gactgggccg gtcacgtcgt cggctcggtc ggcctgtcgc tcttcgccgg aggcgggcgg
     2101 ccgccggatg cggatcgtga cggcgtcccc gacaagcggg acgtctgccc gaacaccccg
     2161 acgagtgcga gggtcgacgc caccggctgt ccgctcgact ccgaccgcga cggcgtgccc
     2221 gacggcctcg accagtgcgc cggcacgccg acgggcgcga cggtgaacgc ggcggggtgc
     2281 ccggccgact cggactcgga tggcgtctat gacgggctcg accagtgcgt caacacgccg
     2341 gccggagcac acgtcgatgc caagggctgc cccgcggacg cggacggcga ccacgtcgcg
     2401 gacgggatcg accagtgccc gaacaccccg gcgggagcga ccgtcgacgc cgcgggatgt
     2461 cccctcgact cggacaagga cggcgtgccc gacgggctgg acaagtgtcc caacacgtcg
     2521 ccgggcatgg aagtggacga cgtcgggtgc cagcgcttca gggacgcc
//
LOCUS       DASPWO010000065         8091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_181400, whole genome shotgun sequence.
ACCESSION   DASPWO010000065 DASPWO010000000
VERSION     DASPWO010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181400"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VEO00_01485"
     CDS             <1..652
                     /locus_tag="VEO00_01485"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HYU56707.1"
                     /translation="DADKREFALGAEAMRSYGFGPARAYDLETVIRHMRPTILVGTSG
                     MAGTFTEGALREMARHAPGPLVFPMSNPTSKTEAHPERVLEWTGGTALVATGSPFEPV
                     ERAGRTHLIGQANNAFVFPGVGLGSMVAEVRQLPDELFLAAAHAVADAVPDDRVAHGA
                     LYPRQADLRAVSRAVAVRVATSARDGGYGRALSDDQIERATAGAMWFPAYRRYRPA"
     gene            complement(669..1214)
                     /locus_tag="VEO00_01490"
     CDS             complement(669..1214)
                     /locus_tag="VEO00_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56708.1"
                     /translation="MLRQRVRRGRAVLVASLLALMVVGLVPTARAAETAIWRGRTSQT
                     LPMKFLVQTESGGRIKAGAFELKLRCGKTGEVQRWFVGFFADPGFRIVDGEFEVDFKD
                     PFAHFRFAGTFLSPTAATGTVDFLIAELTPDDQAQLCGPGAPTWNAEPVPADEDTPTT
                     ATHRIQFRMDARGRVTTTVTS"
     gene            1471..2634
                     /locus_tag="VEO00_01495"
     CDS             1471..2634
                     /locus_tag="VEO00_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HYU56709.1"
                     /translation="MGAVTLGVPRETYPGERRVALTPAAAGDLSAGGFEVLLEAGAGE
                     AAGYPDPAYAERGGRIAPSRDEAFAADAVLQVRTIGANPDAGAPDLERLRPGQVVVGM
                     ADPLGNPEAVRAVAERGATLFALELLPRIARAQSMDVLSSQATVAGYRAAVLAAANLP
                     KLFPMLTTAAGTLPAARVLVLGAGVAGLQAIATARRLGALVEAYDVRPAAREEVESLG
                     ARFVELPLSPGDAEDAGGYAKELSDSFYLRQQEHLASVVTATDAVICTAMIPGKPAPV
                     LLTEEAVAGMALGSVVVDAAAERGGNCAVTRPGETLTVSGVRILGPVNLPADLPREAS
                     QMYARNLTAFLRVLAPEGAFDVASEDEIIRHTLAATGGAVVNHQIPGPEGRAA"
     gene            2631..2978
                     /locus_tag="VEO00_01500"
     CDS             2631..2978
                     /locus_tag="VEO00_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HYU56710.1"
                     /translation="MSDAIEVGLTIFVLATFIGFEVIRRVPGLLHTPLMSLTNSISGI
                     ALVGSLILAGAARGTLATVLGTIAVAASTINVVGGFLITDRMLRMFRRREPRPAAPSA
                     AAADTKAEPGGAR"
     gene            2975..4375
                     /locus_tag="VEO00_01505"
     CDS             2975..4375
                     /locus_tag="VEO00_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HYU56711.1"
                     /translation="MSDQAVSFAYLAAGILFILALKWLSHPLTARRGLLMGEIGMVLA
                     VGSTLMKAEIVDFQWILIALFIGTAIGIPLGQFVPMTAMPQRIAFSHAFGALAAALVG
                     TAEYALRRPEIDRFTMGALGFEVVLGFLTFTGSLIAFGKLQGIVRDKPVLFRGQNVLN
                     LAVLATAVVLVVVLVVDPGLSVVFPVMIGLALLYGVSFVLRIGGADMPVVISLLNSLA
                     GLSGAAMGLVLNNHVLILAGALDGSSGFLLSLIMSRAMNRSFGNVLFGGFGAETTAEG
                     GAGPPRPVRSSSAEEAAMILSAARTVVIVPGFGMAAAQAQHKVRELADLLVRRGTDVK
                     FAIHPVAGRMPGHMNVLLAEANVPYERLYDLEHINPEFPDTDVALVIGANDVVNPAAR
                     HDASSPIYGMPILDVDKANCVMVIKRSMRSGFAGIENELFYLDRTMMLFGDAKAMAGE
                     IVDEVAKLPGAVTAVA"
     gene            complement(4377..4901)
                     /locus_tag="VEO00_01510"
     CDS             complement(4377..4901)
                     /locus_tag="VEO00_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56712.1"
                     /translation="MRRIRTSALVGLVALALVAAPAAPAEARGGNREVWQARTDQHLP
                     LKFSVQTSQGARFIPHHYMVKMRCLESGQRVLAWIGFVGFEVPIKDGEFDFTLRFGEF
                     IQIKGEFLSRTTASGTLAFEFAQVTPDRQAELCTSGDVPWAGRAKPEVEPLADVHFKA
                     TRHADGSVTIERLD"
     gene            complement(4994..5392)
                     /locus_tag="VEO00_01515"
     CDS             complement(4994..5392)
                     /locus_tag="VEO00_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HYU56713.1"
                     /translation="MALPMRRFTLVTIVVLFALLAAAAVYQIVLVSGKPRGFVGPGLS
                     PSAGRLILGDMVGRQRRDAEALLEDAGLDVRVEPRGGDSDIVVAQDPAAGSLVEDGTE
                     VRLAVECVPAPCPTPPQGQEIVDPCGCVTR"
     gene            5419..6870
                     /locus_tag="VEO00_01520"
     CDS             5419..6870
                     /locus_tag="VEO00_01520"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HYU56714.1"
                     /translation="MRRILPFGERSIEADLPDDTRIVGAPPSLPPLKDPAGAVRVALA
                     EPVAHAPLADLVGHGARVTIAFDDPTVTFLGAARPDPRETALGVLLDELTRAGVRPED
                     VRLICANALHRRWTNGELATVVGPVIPYAFGPARFGCHDACDPEQLAYLGMTERGFEV
                     EVSRAILDADQFVYLNVSPSPFNGGWKSTVVGLSSFRSIRHHHRPFPFATGRSVMDPR
                     RSAFPKLLAELGRVLEKSLADRGRRLLQLECVLTSDLPGRPAFIAAGHPPEVHDRTIE
                     ALHRQHVVPVEGQTDALLLALPDADPYSALSHPNPILMANAALGYAFALHMRRPLVHR
                     EGFVIVANPFTLRFHPRHHRSYPEAYEALAATRDAVELWDSHAEDFAARPEYVHAYRY
                     QQSFHGAHPLFLWGQVMRSVAQVAGVLVAGGDPDVCARLGFRAEPSVETALEWVGAQL
                     GRRLSLTWQPLPPLSIPEVRLPGDDGEPGGPDG"
     gene            complement(6874..7698)
                     /locus_tag="VEO00_01525"
     CDS             complement(6874..7698)
                     /locus_tag="VEO00_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HYU56715.1"
                     /translation="MPRLHFSTAPFFRHPLREAFRHVAAGGYSSLEVMVTQDPASQEP
                     HLLLSLAEDHGVSIEVIHAPMLLFTRRVWGTDPVGKIYRAVQVAQEVGASIVVAHPPY
                     RWQVRYQRWIETSLAELSARTGVTVAVENMFPVRVRERGLRFHAAQGQEDLAAYPHLV
                     FDTSHAAVAGLDILEAAHRYRDNLVHVHASNNAGKGWDSHLPVYEGVLPMEEFLRTLR
                     ENGYAGDVSIELDLRPYFDDEDELQKVLAANREFCQEHLGLRAAGDRARATVRAAD"
     gene            7813..>8091
                     /locus_tag="VEO00_01530"
     CDS             7813..>8091
                     /locus_tag="VEO00_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56716.1"
                     /translation="MSERDNRPAWTGGRGRHEVWYLTMSEPSGRAGYWIRYSLHAPTR
                     GEPRARLWFARSDRREPGRTFGINVGAPVEDFVLDGNGFEVSVAGATLR"
BASE COUNT         1073 a         2938 c         2938 g         1142 t
ORIGIN      
        1 cgacgccgac aagcgcgagt tcgccctcgg ggcggaggcg atgcgctcgt acgggttcgg
       61 gccggcccgg gcgtacgacc tggaaaccgt gatccgccac atgcggccga cgatcctcgt
      121 cgggacgagc ggaatggcgg ggacgttcac cgagggcgcg ctccgggaga tggcgcggca
      181 cgcgccgggg ccgctcgtct tcccgatgtc gaacccgacc tcgaagaccg aggcccatcc
      241 ggagcgcgtc ctggagtgga ccggcgggac cgccctggtg gccaccggca gccccttcga
      301 gccggtggag cgcgccgggc ggacacatct gatcgggcag gcgaacaacg cgttcgtctt
      361 ccccggcgtc gggctgggct ccatggtcgc cgaggtccgc cagctgcccg acgagctgtt
      421 cctggcggca gcccacgccg tggcggacgc cgtccccgac gaccgcgtgg cgcacggcgc
      481 gctctatccg cgccaggccg acctccgcgc ggtctcgcgc gcggtggccg tgcgcgtcgc
      541 cacgagcgcc cgcgacggcg gctacgggcg ggccctgtcc gacgaccaga tcgagcgcgc
      601 cacggccggt gcgatgtggt tcccggccta ccgccggtac cggcctgcct gagcccggag
      661 cgaccccgtc agctcgtgac ggtggtggtc accctccccc gggcgtccat ccggaactgg
      721 atgcggtgcg tcgcggtagt cggcgtgtcc tcgtccgccg gcaccggctc ggcgttccag
      781 gtcggcgccc ccggcccgca gagctgagcc tggtcgtcgg gcgtcaactc cgcgatcagg
      841 aagtccaccg tcccggtcgc ggccgtcggg gagaggaacg tcccggcgaa gcgaaagtgg
      901 gcgaacgggt ccttgaagtc gacctcgaac tcgccgtcca cgatccggaa cccgggatcg
      961 gcgaagaacc caacgaacca gcgctgcacc tcgccggtct tcccgcagcg cagcttgagc
     1021 tcgaacgctc ccgccttgat ccggccgccg ctctcggtct gcaccaggaa cttcatcggc
     1081 agcgtctgtg acgttctgcc gcgccagatg gcggtctccg cggcgcgcgc cgtggggacg
     1141 agccccacga ccatcagcgc cagcagcgac gcgacgagaa cggcccgccc gcggcggacc
     1201 cgttgccgga gcatgcggct ctccttccgt gtgggcctcg gccgaggccg gtaggcgcac
     1261 cgtagccccg tgggctcgga cggacaaggg cccagggagg ggaggacgac gagcgggcca
     1321 cggagcgcgg gaccctcgtc cctccgagcc accccggacg gatgacaggc tcgtcgacgg
     1381 gaccgccccc gcgccatcga tatccatgtt cgatgccgcc ggccggccgg tcgggctcgc
     1441 gtgatggctc gcgtgataga aggagaagcg atgggcgccg tgacgctggg agtgccgcgg
     1501 gagacttacc ccggcgagcg gcgggtggcg ctgacgcccg cggcggccgg ggacctgtcc
     1561 gccggggggt tcgaggtcct gctcgaagcc ggggcggggg aggcggccgg gtaccccgac
     1621 ccggcctacg cggagcgcgg cgggcggatc gcaccctcgc gcgacgaggc cttcgccgcc
     1681 gacgccgtcc ttcaggtccg cacgatcggg gcgaaccccg acgccggcgc gcccgacctc
     1741 gagcggctcc ggcccggcca ggtggtggtc ggcatggccg atccgctcgg gaacccggag
     1801 gcggtccggg ccgtggccga gcggggggcg accctgttcg cgctcgagct gctgccgcgc
     1861 atcgcccggg cgcagagcat ggacgtgctg tcctcacagg ccacggtggc cggctaccgc
     1921 gccgccgtgc tcgcggcggc gaacctcccg aagctcttcc cgatgctcac caccgccgcg
     1981 ggcacgctcc cggcggcgcg ggtgctggtg ctcggggccg gggtggccgg gctgcaggcg
     2041 atcgccacgg cccgccgcct gggcgcgctc gtcgaggcgt acgacgtccg cccggccgcg
     2101 cgggaggagg tcgagagcct cggggcccga ttcgtcgagc tgccgctctc gcccggcgac
     2161 gccgaggacg ccggcggcta cgccaaggag ctctcggact cgttctacct gcgccagcag
     2221 gagcacctcg cgagcgtggt gaccgcgacc gacgcggtga tctgcaccgc gatgatcccc
     2281 ggcaagccgg cgccggtgct gctgaccgag gaggcggtcg caggaatggc ccttgggtcg
     2341 gtggtggtcg acgcggccgc cgagcgcggc ggcaactgcg cggtcacacg ccccggcgag
     2401 acgctgactg tgagcggcgt gcggatcctc ggccccgtga acctgccggc ggacctgccg
     2461 cgcgaggcga gccagatgta cgcgcgcaac ctgacggcgt tcctgcgggt gctcgccccg
     2521 gagggcgcgt tcgacgtggc atcggaggac gagatcatcc gccacacgct ggccgccacg
     2581 ggtggcgcgg tggtcaacca ccagatcccg ggaccggagg ggagggcggc atgagtgacg
     2641 ccatcgaggt cggcctgacg atcttcgtcc tggcgacctt catcgggttc gaggtcatcc
     2701 ggcgggtccc gggactgctg cacaccccgc tgatgtcgct gacgaactcg atctccggga
     2761 tcgccctggt cggatcgctg atcctcgccg gcgccgcccg cgggacgctg gccacggtgc
     2821 tcgggacgat cgccgtggcc gcgtccacga tcaacgtggt cggcgggttc ctgatcaccg
     2881 accggatgct gcggatgttc cggcgccgcg agccgcggcc ggccgcgccg tcggccgccg
     2941 cagccgacac gaaggccgag ccgggaggcg cgcgatgagc gaccaggcgg tgtcgttcgc
     3001 gtacctcgcg gccggcatcc tgttcatcct cgccctgaag tggctgtccc acccgctcac
     3061 cgcccggcgc ggcctgctga tgggcgagat cgggatggtc ctcgcggtcg ggagcacgct
     3121 gatgaaggcc gagatcgtcg acttccagtg gatcctgatc gcgctgttca tcgggacggc
     3181 gatcggcatc ccgctcgggc aattcgtgcc gatgaccgcg atgccgcagc ggatcgcctt
     3241 ctcccacgcc ttcggcgcgc tggcggcagc cctcgtcggc accgccgagt acgcgctgcg
     3301 ccggcccgag atcgaccgct tcacgatggg cgcgctcggg ttcgaggtgg tgctcgggtt
     3361 cctgacgttc accggaagcc tgatcgcctt cggcaagctg caggggatcg tccgcgacaa
     3421 gccggtcctc ttccgcggcc agaacgtgct gaacctggcc gtcctggcga cagccgtggt
     3481 gctggtggtc gtcctggtcg tcgaccccgg gctctcggtg gtgttccccg tgatgattgg
     3541 gctggcgctc ctgtacggcg tgtcgttcgt gctgcgcatc ggcggcgccg acatgccggt
     3601 ggtgatctca ttgctgaact ctctggccgg gctgtccggc gcggccatgg gactcgtcct
     3661 caacaaccac gtcctgatcc tggcgggcgc gctcgacggg tcctccggct tcctgctgtc
     3721 gctgatcatg agccgggcga tgaaccgctc gttcgggaac gtgctgttcg gcgggttcgg
     3781 cgccgagacg accgccgagg gcggggccgg tccgccccgt ccggtccgca gctcctcggc
     3841 cgaggaggcg gcgatgatcc tctccgcggc ccgcaccgtg gtgatcgttc ccgggttcgg
     3901 gatggccgcg gcccaggcgc agcacaaggt ccgcgagctg gccgacctgc tggtccgccg
     3961 cgggaccgac gtcaagttcg ccatccaccc ggtcgccggg cggatgcccg gccacatgaa
     4021 cgtgctcctc gccgaggcga acgtccccta cgagcggctg tacgacctgg agcacatcaa
     4081 cccggagttc ccggacaccg acgtcgcgct ggtcatcggg gcgaacgacg tcgtgaaccc
     4141 cgccgcccga cacgacgcgt cgagcccgat ctacgggatg ccgatcctcg acgtcgacaa
     4201 ggcgaactgc gtgatggtga tcaagcgcag catgcgctcg gggttcgccg ggatcgagaa
     4261 cgagctcttc tacctcgacc ggaccatgat gctgttcggc gacgccaagg ccatggccgg
     4321 cgagatcgtc gacgaggtgg cgaagctccc cggcgccgtg accgccgtgg cctgagtcag
     4381 tccagccgct cgatcgtcac cgacccgtcg gcgtggcgcg tcgccttgaa gtgcacgtcg
     4441 gccaggggct cgacctcggg cttggcccgc ccggcccagg ggacgtcacc ggacgtgcac
     4501 agctccgctt ggcgatcggg cgtgacctgc gcgaactcga acgcgagcgt gccgctcgcc
     4561 gtcgtgcggg agaggaactc gcccttgatc tggatgaact cgccgaagcg cagcgtgaag
     4621 tcgaactccc cgtccttgat cgggacctcg aagccgacga agccgatcca ggccaggact
     4681 cgctgcccgg actccaggca ccgcatcttg accatgtagt ggtgcgggat gaaccgcgcg
     4741 ccctgcgacg tctggacaga gaacttcagc ggcaggtgct ggtcggttcg ggcctgccag
     4801 acctcgcggt tcccaccgcg ggcctccgcg ggcgcggccg gggcggcgac gagggccagc
     4861 gcgaccaggc cgaccaacgc ggacgtccgg atgcggcgca tgccggctcc ctccttccgg
     4921 cctcgccgac gaggcctctg ggcgaacggt agccgcgacc gagcggccgc acaaggggcg
     4981 gaagcgacga aggctatctg gtcacgcagc cgcagggatc gacgatctcc tggccctgcg
     5041 gcggggtcgg gcagggggcc ggcacgcatt cgaccgccag gcgcacctcc gtcccgtcct
     5101 cgacgagcga gccggccgcc gggtcctgcg ccaccacgat gtcactgtcg ccgccgcgcg
     5161 gctcgacgcg gacgtcgagg ccggcgtcct cgagcagcgc ctcggcgtcc cggcgctgtc
     5221 ggccgaccat gtcgccgagg atcagccggc cggccgacgg gctgagcccg ggcccgacga
     5281 acccccgcgg cttcccggag acgagcacga tctggtacac ggccgccgcg gcgagcagcg
     5341 cgaagaggac cacgatcgtc accagggtga accgccgcat cgggagggcc atcgccggcg
     5401 caggctagca tccgacccgt gcgacggatc ctgccgttcg gcgagcgatc gatcgaggcg
     5461 gatcttcccg acgacacgcg gatcgtcggg gcgccgccgt cgctcccgcc gctcaaggac
     5521 ccggccgggg cggtccgcgt ggcgctggcg gagcccgtcg ctcacgcccc gctcgccgac
     5581 ctggtcgggc acggcgcgcg ggtcacgatc gccttcgacg acccgaccgt caccttcctc
     5641 ggcgcggcgc ggccggatcc gcgcgagacg gcgctcgggg tcctgctcga cgagctgacc
     5701 cgggccggtg tgcggcccga ggacgtccgc ctgatctgcg ccaacgcgct gcaccggaga
     5761 tggacgaacg gcgagctggc caccgtcgtc gggccggtga tcccctacgc gttcgggccg
     5821 gcgcgcttcg gctgccacga cgcctgcgac cccgagcagc tggcgtatct cggcatgacc
     5881 gagcgcggct tcgaggtcga ggtcagccgc gccatcctcg acgccgacca gttcgtctac
     5941 ctgaacgtca gtccctcgcc gttcaacggc ggctggaagt cgacggtggt cgggctgtcg
     6001 tccttccgtt cgatccgcca ccaccaccgc ccgttcccgt tcgccaccgg gcgttccgtg
     6061 atggacccgc gccggtcggc gttcccgaag ctgctggctg agctgggtcg ggtcctggag
     6121 aagtcgctcg ccgaccgcgg ccgccgcctg ctgcagctgg agtgcgtgct cacctcggac
     6181 ctccccggcc ggccggcgtt catcgccgcc ggccacccgc cggaggtgca cgaccggacg
     6241 atcgaggcgc ttcaccgcca gcacgtcgtc ccggtcgagg gccagaccga cgccctgctg
     6301 ctggcgctcc cagacgcgga cccctactcg gccctctcgc acccgaaccc gatcctgatg
     6361 gcgaacgcgg ccctcggcta cgccttcgcg ctgcacatgc gccggccact cgtgcaccgc
     6421 gaggggttcg tgatcgtggc gaaccccttc acgctccgct tccacccgcg ccaccaccgc
     6481 tcgtacccgg aggcgtacga ggcgctggcg gcaacgcgcg acgcggtgga gctctgggat
     6541 tcgcacgccg aggacttcgc cgcccgtccg gagtacgtgc acgcctaccg ctaccagcag
     6601 tcgttccacg gcgcgcaccc gctcttcctg tggggccagg tgatgcgctc ggtggcgcag
     6661 gtggcgggcg tgctggtggc cggcggcgac cccgacgtgt gcgcgcggct cggcttccgc
     6721 gccgagccga gtgtagagac ggcgctggaa tgggtcggcg cgcagctcgg ccgccggctg
     6781 agcctcacct ggcagccgct gccgccgctc tcgatcccgg aggtccgcct ccccggcgac
     6841 gacggggaac cgggcggccc ggacgggtag ggctcagtcc gcggcgcgaa ccgtcgcccg
     6901 ggcgcggtct cccgccgccc gcaggccgag gtgctcctgg cagaactcgc ggttcgccgc
     6961 gagcaccttc tgcagctcgt cctcgtcgtc gaagtaggga cggaggtcga gctcgatcga
     7021 cacgtcgccg gcgtaaccgt tctcccgcag cgtgcgcagg aactcctcca tcggcagcac
     7081 gccctcgtag accgggaggt ggctgtccca gcccttcccg gcgttgttgc tggcgtggac
     7141 gtggaccagg ttgtcgcggt agcggtgggc cgcctcgagg atgtccaggc cggccacggc
     7201 cgcgtggctc gtgtcgaaga ccaggtgcgg gtaggcggcc aggtcctcct gcccctgcgc
     7261 ggcgtggaag cgcagccccc gctcgcggac gcgcaccggg aacatgttct cgaccgccac
     7321 cgtcacgccc gtgcgcgccg acaactcggc caggctcgtc tcgatccagc gctggtagcg
     7381 cacctgccag cggtatgggg gatgggcgac cacgatcgag gctcccacct cctgggccac
     7441 ctggaccgcc cggtagatct tgccgaccgg gtcggtgccc cacacccggc gggtgaacag
     7501 gagcatcggg gcgtggatca cctcgatcga gacgccgtgg tcctccgcca ggctcagcag
     7561 caggtgcggc tcctgcgagg cggggtcctg ggtgaccatc acctccaggc tcgaatagcc
     7621 gccggcggcc acgtgtctga aggcctctcg caggggatgg cgaaagaagg gtgcggtgga
     7681 gaagtggagc cgcggcacgg ccttccggtc gggagtgatc gtcggtacct cgtcggtcgg
     7741 gtcgccataa tgatacgggc cgtggcctgg agcgcccggg agaaagggtg ctcgccctgg
     7801 ggtgacgtgc gcgtgagcga gagggacaac aggccggcct ggaccggcgg ccgcggccgc
     7861 cacgaggtct ggtacctgac gatgtccgaa ccgtccgggc gcgccgggta ctggatccgc
     7921 tactccctgc acgcaccgac gcgcggggag ccccgagccc ggctgtggtt cgcccggtcc
     7981 gaccgccggg aacccgggcg gacgtttggt atcaacgtcg gagctccggt ggaggacttc
     8041 gtcctcgacg ggaacggctt cgaggtctcg gtggccgggg ccaccctgcg c
//
LOCUS       DASPWO010000066         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_181496, whole genome shotgun sequence.
ACCESSION   DASPWO010000066 DASPWO010000000
VERSION     DASPWO010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181496"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..338)
                     /locus_tag="VEO00_01535"
     CDS             complement(126..338)
                     /locus_tag="VEO00_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56717.1"
                     /translation="MTGLNAVRWLRQARKASRELRARGEPFPLADAFFVDRPTRDLLD
                     ALIGLGILERQDLANAPGHGPLVSAA"
     gene            477..1400
                     /gene="ilvE"
                     /locus_tag="VEO00_01540"
     CDS             477..1400
                     /gene="ilvE"
                     /locus_tag="VEO00_01540"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HYU56718.1"
                     /translation="MPIEKVEKVWMDGELVDWDRATVHVLTHTLHYGSGVFEGIRCYE
                     TKRGPAIFRLRDHIARLYRSAAIYMIDIPYAPEELIEAAKATVRVNGLRECYVRPIVY
                     LGYGEMGLNPLPAPVNVAIAVWHWGAYLGEECIDHGCRAKISSWVRHDPNIIPAAAKG
                     IGQYVNSSLAKIEAIKAGYDEAIMLNPHGYVAEGTGENIFIVRDGAVVTPPLSAGPLG
                     GLTRDSAMTIARDRGYDVIEADLMRTDLYTADEAFFTGTAAEITPIREIDDRTIGSGD
                     RGPITKELQETFFGAIRGEIPEYDRWLDHVG"
     gene            1419..>2071
                     /locus_tag="VEO00_01545"
     CDS             1419..>2071
                     /locus_tag="VEO00_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYU56719.1"
                     /translation="MRHPGGIGTGFVDGETVRLMRGTFFDEPVPSGDAFALASVRLLA
                     PVIPSKVVAVGKNYAEHAEEFGGGVPEEPILFLKPSTAVIGPGDPIPLPAESERVDHE
                     AELAVVIGRLARRVAIEEAGKHVLGYTCANDVTARDLQERDGQWARAKGFDGFCPLGP
                     WIETELDPLDVALECRVNGETRQAARTSQLSFGPGELIAFVSAVMTLLPGDVILTGTP
                     "
BASE COUNT          305 a          694 c          777 g          295 t
ORIGIN      
        1 ccaccagcgc cgagaaggcg agcagggtcg tggcgtccgg tcgggtggtc tcggcgtcgg
       61 gcgtcacgac cgctaccgta cccctcgttc cgggtcgcgg ccgcgcaggg tcgggcgggt
      121 acggctcagg cggccgagac gagcggaccg tggccggggg cgttcgccag gtcttgccgt
      181 tccaggatgc cgagcccgat cagcgcgtcg agcaggtcgc gggtcgggcg atcgacgaag
      241 aacgcgtcgg ccagcgggaa cggctcaccc cgggcgcgca gctctcggga ggccttccgg
      301 gcctggcgca gccaccgcac ggcattgagt ccagtcatgc gttcagcctt ccccggccac
      361 ggttcgtcga aacggccgga cggcactttc gagccggtcc ggacggccgc cgcgtccggg
      421 cgccgcggtt cggtatcctt cgctgcgagg gccttccccc gtcagggagg agcggaatgc
      481 cgatcgagaa ggtcgagaag gtctggatgg acggtgagct ggtcgattgg gaccgggcga
      541 ccgtccacgt gctcacgcac acgctgcact acgggtcggg cgtgttcgag ggcatccgct
      601 gctacgagac gaagcgcggg ccggccatct tccggctgcg cgaccatatc gcgcgcctct
      661 atcggtcggc cgcgatctac atgatcgaca tcccgtacgc gcccgaggag ctgatcgagg
      721 cggccaaggc gaccgtgcgc gtcaacgggc tgcgggagtg ctacgtgcgg ccgatcgtgt
      781 atctcgggta cggcgagatg gggctcaacc cgctgccggc gccggtgaac gtggccatcg
      841 cggtgtggca ctggggcgcc tacctcggcg aggagtgcat cgatcacggc tgccgggcca
      901 agatctcctc ctgggtgcgg cacgacccga acatcatccc ggccgcggcg aaggggatcg
      961 gccagtacgt caactcgagc ctggccaaga tcgaggccat caaggcgggc tacgacgagg
     1021 cgatcatgct caacccgcac ggctacgtgg ccgagggaac cggcgagaac atcttcatcg
     1081 tccgcgacgg cgccgtggtg acgccgccgc tctcggccgg accgctgggc ggcctgacgc
     1141 gggactcggc gatgacgatc gcccgcgacc gcgggtacga cgtcatcgag gccgacctga
     1201 tgcgaaccga cctctacacg gccgacgagg ccttcttcac gggtaccgcg gccgagatca
     1261 ccccgatccg cgagatcgac gaccgcacca tcggctcggg cgaccgcggc ccgatcacca
     1321 aggagctgca ggagacgttc ttcggcgcga tccgcggcga gatccccgag tacgaccgct
     1381 ggctcgacca cgtcgggtag gccgtgaggc tcgtccgggt gcggcatccc ggcgggatcg
     1441 ggactgggtt cgtcgatggc gagacggtgc ggctgatgcg gggaaccttc ttcgacgagc
     1501 cggtgccgtc cggcgacgcg ttcgcgctgg cgtccgtgcg cctgctggcg ccggtgatcc
     1561 cgtcgaaggt ggttgccgtc gggaagaact acgccgagca cgccgaggag ttcggcgggg
     1621 gggtgccgga ggagcccatc ctgttcctga agccctccac ggccgtgatc gggccggggg
     1681 accccatccc gctgccggcg gagagcgagc gggtggacca cgaggccgag ctggcggtgg
     1741 tcatcgggcg gctggcccgg cgcgtggcga tcgaggaggc cggcaagcac gtgctcggct
     1801 acacgtgtgc caacgacgtc acggcgcgcg acctgcagga gcgcgacggg cagtgggccc
     1861 gggcgaaggg gttcgacggg ttctgtcctc tcggcccgtg gatcgagacc gagctcgacc
     1921 cgctcgacgt cgcgctggag tgccgggtga acggcgagac gcgccaggcg gcccggacgt
     1981 cccagctctc gttcgggccc ggcgagctga tcgccttcgt cagcgcggtg atgaccctcc
     2041 tgcccggcga cgtgatcctc acggggacgc c
//
LOCUS       DASPWO010000067         6046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1196693, whole genome shotgun sequence.
ACCESSION   DASPWO010000067 DASPWO010000000
VERSION     DASPWO010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196693"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..303)
                     /gene="purS"
                     /locus_tag="VEO00_01550"
     CDS             complement(13..303)
                     /gene="purS"
                     /locus_tag="VEO00_01550"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HYU56720.1"
                     /translation="MTVRRFAVTVTLKEGLLDPQGETVKGALPTMGWGNVTEVRVGKH
                     IELAVEVEDAAGARAQVEEMAARFLTNPVIEDFRVEELPAEAGPSENGQGTS"
     gene            complement(300..1268)
                     /locus_tag="VEO00_01555"
     CDS             complement(300..1268)
                     /locus_tag="VEO00_01555"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HYU56721.1"
                     /translation="MTTGGFGGGARPGSRLARPRSASEPLKEEGLFARGKVRDIYEAG
                     HDLLMVASDRISAFDVVLPTAIPDKGRVLTALSLFWFERTADLTANHLLTADATAFPE
                     PFRGRADLAGRAMLVRKAEMVPIECVARGYLSGSGWKEYRASGTVCGIALPAGLVESD
                     RLPEPIFTPATKAVTGHDENIPLDRAAEIVGEGLAQRLKELTLAIYERAAEVARARGI
                     MVADTKLEFGFVDGELVLADEVLTPDSSRFWPADGHRPGGAQPSFDKQYVRDWLDASG
                     WDHEPPPPDLPADVALQTAARYREAYERLSGEPFADFLARMRTDRA"
     gene            complement(1265..2581)
                     /gene="purB"
                     /locus_tag="VEO00_01560"
     CDS             complement(1265..2581)
                     /gene="purB"
                     /locus_tag="VEO00_01560"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HYU56722.1"
                     /translation="MIPRYALPEMAAVWSDEARMGHWLRIEVLAAEAWGRLGLIPPED
                     LVAIRDRAAFTVEAVREAERVTNHDVSAFVDVVARSVGPAGRWIHHGLTSSDVLDTAL
                     ALQLRAAVDLLLARLTRLLAVVRRLALEHRDTLMVGRTHGVHAEPTTFGHVAALWAFE
                     LDRDRERLRRARRAVSVGKISGVVGTYAQVDPAVEEHVCAELGLRPVEADSQVIRRDR
                     HAEFVWALAVTAATLETIVLEVRHLARTEVREVQEPFEAGQKGSSAMPHKRNPIVAER
                     ISGLARVIRANLAPALEDVPLWHQRDISHSSVERVILPDSTIALDYLLHLATGLLGGL
                     VVFPERMRANLFASRGVVFSQSVLLALVERGMPREAAYRIVQRAAAVAWDGGGDFRSL
                     LAADPEVEATLAPEDLDALFDPTRTLGRLDGVFARVEKLDVTEEAG"
     gene            complement(2616..3089)
                     /gene="purE"
                     /locus_tag="VEO00_01565"
     CDS             complement(2616..3089)
                     /gene="purE"
                     /locus_tag="VEO00_01565"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HYU56723.1"
                     /translation="MAEVTPLVGILTGSASDLPVMQQAGAILERFEVPHELHVMSAHR
                     NPDAVDAYASMAEERGLRVLICGAGMAAHLAGAVAARTTLPVIGVPILSDTLGGADAL
                     FATVQMPRGVPVATVAIGGAVNAAILAVQILATSDESLRVRLREFKQSLGEGLKI"
     gene            complement(3093..4361)
                     /gene="purD"
                     /locus_tag="VEO00_01570"
     CDS             complement(3093..4361)
                     /gene="purD"
                     /locus_tag="VEO00_01570"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HYU56724.1"
                     /translation="MRILVVGGGGREHAIASRLSRNPTVDRLYAAPGNAGIAELAVCV
                     PIAAGDVAGLADFAEREQVDLTVVGPEAPLVAGLADELGSRGLAVCGPTRAAAMLEGS
                     KSWARELCERHGIPGPRARSFTAFDEAVAYLDELAAPYVVKADGLAGGKGVVVAESLS
                     EAETALRACLLDRVFGDAGMRVLVEEHLEGEEVSALALTDGRDVLPLTLAQDFKRALD
                     GDRGPNTGGMGAYSPVSFVDPATAGFIEDRILRATVAALEREGLRYRGVLYAGVMLTA
                     DGPKVLEFNCRFGDPETEAILPRFGSDVAESLLACAEGNLGHYRLSWDERPCVTVVVA
                     MKGYPGAHPTGLPIEGLQEARAVDGVEVFHAGTARKDGRVVTSGGRVLAVSALGADHA
                     EARARAYEACSRIRFEGMRYRRDIAERVAG"
     gene            complement(4362..5639)
                     /locus_tag="VEO00_01575"
     CDS             complement(4362..5639)
                     /locus_tag="VEO00_01575"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HYU56725.1"
                     /translation="MPATILVGTQWGDEGKGKATDLLADEMDFVVRYQGGNNAGHTVL
                     AGEHRLKLQLVPSGILYPHITCVIGDGVVVDPGFLLHEMETLAGRGIDVSRLRVSPNA
                     HLIMPYHLELDRVTERYLGKHALGTTKRGIGPAYADKAARIGLRVQDLTDEKIFREKL
                     DVALREKNLVLTKVYNRLPLSAEEIVDAYLEYGRRLAPHIADTSGMLWNALREGRRVL
                     LEGAQGTLLDLDHGTYPFVTSSNPVAGGALVGSGLGPREVDRVIGVTKAYVTRVGAGP
                     FPTEDTGEAGERMVNAGEEYGTVTGRRRRCGWFDAVLLRYAARVNGLTELFLTKLDVL
                     SGLGRLRLCVAYRYEGRSYEDFPPHQTIVHKAEPVYEDVEGWWEPLGEARAYGDLPEA
                     ARRYVERIGELGGVPVATVSVGPDRAASLLVAA"
     gene            complement(5704..5907)
                     /locus_tag="VEO00_01580"
     CDS             complement(5704..5907)
                     /locus_tag="VEO00_01580"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HYU56726.1"
                     /translation="MSVIKTIDLVGVSGESWRDAAAQALAEASKTLRGIEGMDILETS
                     ATVDGDQIKEYHTHVRIKFRIER"
BASE COUNT          835 a         2339 c         2048 g          824 t
ORIGIN      
        1 gcgggcgccg ggtcacgatg tcccctgccc gttctcggac ggtccggcct cggcgggcag
       61 ctcctcgacc cggaagtcct cgatcaccgg gttcgtcagg aagcgagccg ccatctcctc
      121 gacctgcgcc cgggcgccgg cggcgtcctc gacctccacc gccagctcga tatgcttgcc
      181 gacgcgcacc tcggtgacgt tcccccagcc catggtcggc agggcaccct tcaccgtctc
      241 cccctgcggg tccagcaggc cctctttcag cgtcaccgtc accgcgaagc gtcgcacggt
      301 catgcccgat cggttcgcat cctcgccagg aaatcggcga acggctcgcc cgacagccgc
      361 tcgtaggcct ctcggtagcg ggcggcggtc tgcagggcga cgtcggccgg caggtcgggc
      421 ggtggcggct cgtggtccca gcccgaggcg tcgagccagt cgcggacgta ctgcttgtcg
      481 aacgagggct gggcgccgcc cggccggtgg ccgtcggccg gccagaatcg cgaggagtcc
      541 ggcgtcagga cctcgtcggc cagcaccagc tcgccgtcga cgaacccgaa ctcgagcttc
      601 gtgtcggcga ccatgatccc gcgggcccgg gcgacctccg cggcccgctc gtagatcgcg
      661 agcgtcagct ccttcagccg ctgtgccagg ccctcgccga cgatctcggc cgcgcgatcg
      721 agcgggatgt tctcgtcgtg gccggtcacg gccttcgtcg ccggagtgaa gatcggctcc
      781 ggcaggcggt cggactcgac gagcccggcc ggcagcgcga tcccgcacac ggttcccgac
      841 gcacggtact ccttccaccc cgaccccgac aggtagccgc gcgccacgca ctcgatcggg
      901 accatctcgg ccttccggac gagcatcgcc cgcccggcca ggtcggcccg cccacggaac
      961 ggctccggga acgcggtcgc gtcggccgtc agcaggtggt tcgccgtcag gtcggccgtc
     1021 cgctcgaacc agaagagcga cagcgcggtc agcacgcggc ccttgtccgg gatcgcggtc
     1081 ggcaggacca cgtcgaacgc cgagatccgg tcgctcgcca ccatcagcag gtcgtgcccc
     1141 gcctcgtaga tgtcgcggac cttcccccgg gcgaagagtc cttcttcctt gaggggttcg
     1201 ctcgccgaac gtggtcgggc aagacggctc ccagggcgag ctcccccccc aaaccccccg
     1261 gtcgtcaccc cgcctcctcg gtcacgtcga gcttctcaac gcgcgcgaac acgccgtcga
     1321 gccggccgag cgttcgcgtc gggtcgaaca gcgcgtccag gtcctccggc gccagggtcg
     1381 cttccacctc ggggtcggcg gccagcagcg agcggaagtc gccgccgccg tcccaggcca
     1441 cggcggccgc gcgctggacg atgcggtacg ccgcctcccg gggcatgccg cgctcgacga
     1501 gcgccaggag caccgactgc gagaacacga cgccgcggga ggcgaacagg ttcgcccgca
     1561 tgcgctcggg gaagaccacc aggcccccca ggagcccggt cgccaggtgc aggaggtagt
     1621 cgagcgcgat cgttgagtcg ggcaggatca cgcgttccac cgaggagtgc gagatgtcgc
     1681 gctggtgcca cagcgggacg tcctcgagcg ccggggccag gttcgcccgg atcacccggg
     1741 ccaggccgga gatccgctcg gcgacgatcg ggttgcgctt gtgcggcatc gccgaggagc
     1801 ccttctgccc cgcctcgaac ggctcctgga cctcacgcac ctcggtccgc gccaggtggc
     1861 gaacctcgag gacgatggtc tccagggtgg cggccgtcac ggccagcgcc cagacgaact
     1921 cggcgtgccg gtcgcgccgg atcacctggc tgtcggcctc caccggccgc agcccgagct
     1981 ccgcgcagac gtgctcctca accgctgggt ccacctgcgc gtaggtcccg acgacgccgg
     2041 agatcttgcc caccgagacc gcccgccgcg cgcggcgcag gcgctcccgg tcgcggtcga
     2101 gctcgaaggc ccacagggcg gcgacgtggc cgaacgtggt cggctcggcg tgcacgccgt
     2161 gcgtgcgccc gaccatcaac gtgtcgcggt gctcgagggc cagccggcgg accaccgcca
     2221 gcaggcgggt cagtcgcgcc aggagcaggt cgacggccgc gcgcagctgc agcgcgagcg
     2281 ccgtgtcgag cacgtcggag gacgtcagcc cgtggtggat ccatcgcccg gccggcccga
     2341 ccgagcgcgc cacgacgtcg acgaacgcgg acacgtcgtg gttcgtgaca cgctcggcct
     2401 cgcgcaccgc ctcgaccgtg aacgcggcgc ggtcgcggat cgccacgagg tcctcggggg
     2461 ggatcagccc gagccggccc caggcctcgg cggccagcac ctcgatccgc agccagtggc
     2521 ccatccgcgc ctcgtcggac cagaccgcag ccatctcggg gagcgcgtac cgcgggatca
     2581 cgccgttccc cccgcgttcc ccgccaggcc acccactaga tcttcagccc ttcgccgagc
     2641 gactgcttga actcccggag gcggacgcgc agggactcgt cgctcgtggc caggatctgc
     2701 accgccagga tcgcggcgtt cacggcgccg ccgatggcga cggtggcgac cggcacgccg
     2761 cgtggcatct gcacggtggc gaacagcgcg tctgccccgc cgagcgtatc ggagaggatc
     2821 ggcaccccga tcaccgggag cgtggtccgg gccgcgaccg ccccggccag gtgcgccgcc
     2881 atcccggcgc cgcagatcag cacccgcagc ccgcgctcct cggccatcga ggcgtaggcg
     2941 tccacggcgt cggggttgcg gtgggcggac atcacgtgca gctcgtgtgg gacctcgaag
     3001 cgctccagga tggcccccgc ctgctgcatc acggggaggt cggatgccga accggtgagg
     3061 atgccgacga gcggggtgac ctcagccacc gactagcccg ccacccgttc ggcgatgtcc
     3121 cgccggtatc gcatcccctc gaagcggatc cgggagcacg cctcgtaggc gcgcgcccgg
     3181 gcctccgcgt ggtcggcgcc gagggcgctc accgcgagca cgcgccctcc ggacgtgacc
     3241 actctaccgt ctttgcgcgc ggtacccgcg tggaacacct ccacgccgtc gaccgcgcgc
     3301 gcctcctgca atccctcgat cggcaggccg gtcggatgcg cccccgggta ccccttcatc
     3361 gccaccacca ccgtgacgca gggacgctcg tcccaggaca gccggtagtg gcccaggttc
     3421 ccctcggcgc aggcaagcag cgactcggcg acgtcgctgc cgaaccgtgg gaggatcgcc
     3481 tcggtctccg gatccccgaa gcggcagttg aactccagca ccttgggccc gtcggccgtc
     3541 agcatcaccc cggcgtagag caccccccgg tagcgcaggc cctcgcgttc gagggccgcg
     3601 acggtcgccc gcaggatgcg gtcctcgatg aaccccgccg tcgccgggtc cacgaaggac
     3661 accgggctgt aggcgcccat ccccccggta ttcggccccc ggtccccgtc gagcgcccgc
     3721 ttgaagtcct gcgccagcgt aaggggcagc acgtcgcggc cgtcggtcag cgcgagcgcc
     3781 gagacctcct cgccctcgag gtgctcctcg accagtaccc gcatgccggc gtcgccgaag
     3841 acccggtcaa ggaggcaggc ccgcagcgcc gtctcggcct cggacagcga ctccgccacc
     3901 accacgccct tcccgcccgc caggccgtcg gccttcacca catacggcgc cgccagctcg
     3961 tcgaggtagg cgacggcctc gtcgaaggcg gtgaagctcc tggcccgcgg gccggggatc
     4021 ccgtggcgct cgcacagctc ccgggcccag gacttcgacc cctcgagcat cgccgcggcg
     4081 cgggttggcc cgcacaccgc cagcccgcgc gagccgagct cgtccgccag gccggccacg
     4141 agcggcgcct cgggcccgac gacggtcagg tcgacctgtt cccgttcggc gaagtcggcc
     4201 agcccggcga cgtcgccggc ggcgatcggg acgcacacgg cgagctccgc gatgccggcg
     4261 ttcccgggag cggcgtacag ccggtcaacc gtcgggttcc tcgacagccg cgacgcgatg
     4321 gcgtgctcgc ggcccccgcc gccgacgacg aggatgcgca tctacgcggc caccagcagg
     4381 ctcgccgcgc ggtcgggccc caccgagacc gtcgcgaccg gcacgccgcc gagctcgccg
     4441 atccgctcga cgtagcgccg ggcggcctcg ggcaggtcgc cgtacgcccg ggcctcgccg
     4501 aggggctccc accagccctc cacgtcctcg tacacgggct cggccttgtg cacgatggtc
     4561 tggtgcggcg ggaagtcctc gtacgagcgg ccctcgtagc ggtacgccac gcacaggcgc
     4621 agccgcccca ggccggacag cacgtcgagc ttcgtcagaa acagctcggt caggccgttg
     4681 acgcgggcgg cgtagcgcag caggacggcg tcgaaccagc cgcagcgccg acggcgcccg
     4741 gtaacggtcc cgtactcctc gccggcattc accatccgct cgccggcctc gcccgtgtcc
     4801 tcggtcggga agggcccggc cccgacccgg gtcacgtacg ccttcgtcac cccgatcacg
     4861 cggtccacct cgcgcggacc gagccccgaa cccacgagcg cgccgccggc gacggggttc
     4921 gatgacgtca cgaacggata ggtgccgtgg tcgaggtcga gcagcgtccc ctgcgcccct
     4981 tccagcagga cgcgcctgcc ttcgcggagc gcgttccaga gcatccccga ggtgtcggcg
     5041 atgtgcggcg ccaggcgccg cccatactcg aggtacgcgt cgacgatctc ctccgccgag
     5101 agcggcaggc ggttgtagac cttcgtcagc accaagttct tctcgcgcag ggccacgtcg
     5161 agcttctcgc ggaagatctt ctcgtcggtc aggtcctgga cccgcaggcc gatccgcgcc
     5221 gccttgtcgg cgtacgccgg gccgatcccg cgcttggtgg tccccagcgc gtgcttgccc
     5281 aggtagcgtt cggtgacccg gtcgagctcc aagtggtagg gcatgatcag gtgggcgttc
     5341 ggcgagaccc gcaggcgcga cacgtcgatc ccccgaccag cgagcgtctc catctcatgc
     5401 agcaggaacc cggggtcgac taccacgccg tcaccgatca cgcaggtgat gtgcgggtag
     5461 aggatcccgg acggcacgag ctgcagcttc agccgatgct cgccggcgag cacggtgtgg
     5521 ccggcattgt tccccccctg gtagcgcacg acgaagtcca tctcgtcggc gagcaggtcg
     5581 gtcgccttcc ccttgccctc gtccccccac tgcgtcccga cgaggatcgt ggcaggcacg
     5641 tcggaacctc tcctcctcct tgccttcggg gggcccacgg gggggcagag tcccccgtgc
     5701 tgatcagcgc tcgatccgga acttgatccg gacgtgcgtg tggtactcct tgatctgatc
     5761 cccgtccacg gtggccgagg tctccaggat gtccatcccc tcgatgccgc gcagggtctt
     5821 cgacgcctcg gcgagggcct gcgccgcggc gtccctccag gactcccccg agacgcccac
     5881 caggtcgatc gtcttgatga cggacacgtg cgccccctcc tctcggaccg gcgtgaatcg
     5941 cgagtcgcgc gccgtgattc tagggacctc ccgcgtgtgg gctcaaacct gcggttcccg
     6001 cgcccgactc aggcgcgcgc gacggcctcc tcaacctggc gttccc
//
LOCUS       DASPWO010000068         3914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1197209, whole genome shotgun sequence.
ACCESSION   DASPWO010000068 DASPWO010000000
VERSION     DASPWO010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197209"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1399
                     /gene="murC"
                     /locus_tag="VEO00_01585"
     CDS             17..1399
                     /gene="murC"
                     /locus_tag="VEO00_01585"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HYU56727.1"
                     /translation="MPTLPVPDVSALRSVHLVGIGGAGMSGLARLFLARGVSVGGSDL
                     KQSAGIERLRELGARVAVGHDASLVEGVDAVVVSSAIPPTNPEVERARALGIAVLARA
                     QALAGLLADRDTLAVAGTHGKTTTTSMAAVVLERAGLDPTYVIGGDLNESGSNARAGG
                     GRIAVAEADESDGSFLLLTPAIGIVTNVEVDHVDFYPGGAQEVEAAFRAFCERCTVVV
                     ACAEDAGVRRVVAGLGNVVTYGTAGEADVRVELSGEPGRARVVLDGSAVELSLPIPGR
                     HNLLNAAAVLAAAARVGVAPADGAASLAGFTGVRRRFESRAEAGGARFVDDYAHHPTE
                     IAATLAAARDRSSARLVAVFQPHRYSRTEAMWAALGRSLAGADVVVVTDVYGAGEQPI
                     PGVTGHLVVESLLEEAPSKRVAYLPRRHDLSAYLAREVRAGDLVLTLGAGDITTLGDE
                     VAQRLREELP"
     gene            1396..2343
                     /gene="murB"
                     /locus_tag="VEO00_01590"
     CDS             1396..2343
                     /gene="murB"
                     /locus_tag="VEO00_01590"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HYU56728.1"
                     /translation="MSAPREAVDRADDILGRAAGDRVRRDYPLAPLTTFRIGGPAALY
                     LEAAGDGDLAAAGHAAGESGLPTLLIGKGSNVLVGDGGFPGIVIRLGRAYRWAAREGD
                     DLVAGGAMPLPALSGVALAERLAGLEFGVAIPASLGGAVKMNAGAHGRSVSEVLAAVE
                     LFGLDDGTRRFIPAAEAGFAYRTSRLPESAVVVGARVRLAPGEAGAIRALMDEARRWR
                     RDTQPLGEPNCGSVFKNPERDHAARLVEAAGGKGLRVGGAAVSTKHANFIVAGDGARA
                     ADVVHLIRSLQERVRERFGVELEPEVRFVGDFEDRPEAA"
     gene            2501..3103
                     /locus_tag="VEO00_01595"
     CDS             2501..3103
                     /locus_tag="VEO00_01595"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HYU56729.1"
                     /translation="MRRIEVHGNGRLTDARVARLAAVPASANLIRLSTAAVEARLEAH
                     PWIAAATVRRTLPGTLDVLVRERGPDAAIRAGKGWALLAADGTVLGTRDRRPDLPMIG
                     ARVSGAAPGARLVGLLPPLRVLAHLPDAVPAVRARPDGEGSVVLVLSGGTRVRYGEAQ
                     GWEQKNPAIEALLAWADRHGVAAARIDVRVPQSPALAPAG"
     gene            3327..>3914
                     /locus_tag="VEO00_01600"
     CDS             3327..>3914
                     /locus_tag="VEO00_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HYU56730.1"
                     /translation="MQGPQNYLAVIKVVGIGGGGVNAVNRMIDAGLKGVEFIAINTDA
                     QALLMSEADVKLDIGRETTRGLGSGSNPDVGLRAAEEHRTEIEEILKGADMVFITAGE
                     GGGTGTGGAPLVAEIARDMGALTIGVVTRPFSFEGRKRATQADMGIADLKKAVDTLII
                     VPNDRLLQVSDPLTPMVDAFRMADQVLYQGVDGITS"
BASE COUNT          474 a         1388 c         1543 g          509 t
ORIGIN      
        1 gccgcggccg gggacgatcc cgacgctgcc ggtgccggac gtctcggcgc tgcggtcggt
       61 gcacctggtc gggatcggcg gcgcggggat gagcgggctc gcccggctgt tcctggcgcg
      121 cggcgtctcg gtcggcggct cggacctgaa gcagtcggcc gggatcgagc ggcttcgcga
      181 gctcggggcg cgggtggcgg tgggccacga cgcgagcctg gtggaaggcg tggacgccgt
      241 ggtcgtctca tcggccatcc cgccgacgaa cccggaggtc gagcgggcac gggcgctcgg
      301 catcgcggtc ctggcgcggg cccaggccct ggccggcctg ctggccgacc gcgacaccct
      361 cgcggtggcc ggcacccacg gcaagaccac caccacgtcc atggcggcgg tcgtcctgga
      421 gcgcgccgga ctcgatccga cgtacgtgat cggtggcgac ctgaacgaga gcggctcgaa
      481 cgcccgcgcc ggcggggggc ggatcgccgt ggcggaggcc gacgagagcg acggctcctt
      541 cctgctcctc accccggcga tcgggatcgt cacgaacgtc gaggtggacc acgtcgactt
      601 ctatcccggg ggcgcccagg aggtcgaggc ggccttccgc gcgttctgcg agcggtgcac
      661 cgtcgtggtg gcctgcgccg aggacgccgg ggttcgccgc gtcgtcgccg gcctggggaa
      721 cgtcgtcacg tacggcaccg ccggcgaggc cgacgttcgg gtcgagctct ccggcgagcc
      781 ggggcgcgcc cgggtggtcc tggacgggtc ggcggtcgag ctgtcgctcc cgatccccgg
      841 ccgacacaac ctgctgaacg cggccgccgt cctggcggcg gcggcgcgcg tcggcgtggc
      901 gccggcggac ggggccgcct ccctggcggg cttcaccggc gtgcgccgcc gcttcgagtc
      961 gcgggccgag gccggtgggg cgcgattcgt cgacgactac gcgcatcacc cgaccgagat
     1021 cgcggccacg ctcgcggcgg cgcgcgaccg ctcctccgcc cggctggtgg ccgtcttcca
     1081 gccgcaccgg tactcgcgga ccgaggcgat gtgggcggcc ctcggccgca gcctggccgg
     1141 cgcggacgtc gtcgtggtca ccgacgtgta cggggccggc gagcagccga tccccggggt
     1201 caccgggcac ctcgtggtcg agtccctgct cgaggaggcg ccgtcgaagc gcgtcgcgta
     1261 cctgccgcgc cggcacgacc tgagcgccta cctggcgcgg gaggtccgcg ccggcgacct
     1321 cgtgctgacg ctcggcgccg gagacatcac cacgctcggg gacgaggtgg cgcagcggct
     1381 ccgggaggaa ttgccgtgag cgcaccgcgg gaggcggtcg atcgcgcgga cgacatcctg
     1441 ggacgggcgg ccggcgaccg ggtccggcgc gactacccgc tcgcccccct caccacgttc
     1501 cggatcgggg gccccgcggc cctctacctc gaggcagcgg gcgacgggga cctggccgcc
     1561 gccgggcacg ccgccggtga gtcggggctc ccgaccctct tgatcggcaa gggctccaac
     1621 gtcctcgtcg gggacggcgg gttcccgggc atcgtcatcc gcctcggccg cgcgtatcgc
     1681 tgggccgcgc gcgaggggga cgacctggtg gccggcgggg cgatgccgct gccggcgctc
     1741 tcgggtgtgg cgctcgccga gcgcctggcg gggctcgagt tcggtgtggc catccccgcg
     1801 tcgctcggcg gcgcggtgaa gatgaacgcc ggcgcacacg ggcgttcggt gtcggaagtc
     1861 ctcgctgcgg tcgagctgtt cgggctcgac gacgggacgc ggcggttcat ccctgccgcc
     1921 gaggccgggt tcgcctaccg cacgtcgcgc ctccccgagt cggcggtggt cgtcggcgcc
     1981 cgcgtccgcc tcgcgccggg ggaagcgggc gcgatccgcg cgctgatgga cgaggcgcgc
     2041 cgctggcgcc gcgacacgca gccgctcggc gaaccgaact gcggcagcgt gttcaagaac
     2101 ccggagcgcg accacgcggc gcgcctggtc gaggccgcgg ggggaaaggg cctgcgcgtc
     2161 ggaggggcgg cggtctcgac gaagcacgcc aacttcatcg tcgccggcga cggggcgcgc
     2221 gccgccgacg tggtgcacct gatccgatcg ttgcaggagc gggtccgcga gcgcttcggc
     2281 gtggaactcg agccggaggt gcgattcgtt ggggacttcg aggaccggcc ggaagcggcg
     2341 tagctcccgt cgcgcggagc ctgcgggacg cgccgcgcgc ccggcgcgcc atgtccgccc
     2401 gccgcgacgg cggcgcccga cccgctgggc gtcggcgatc gtcgctctcg gcgcgatcgc
     2461 cggcggctcg tggctggcgc tcagctcgtc gttgttcgcc gtgcgccgga tcgaggtgca
     2521 cggcaacggt cgcctgacgg acgcgagggt ggcgcggctc gcggcggtgc cggcgtccgc
     2581 aaacctcatc cgcctgtcga ccgcggcggt cgaggcccga ctggaggcgc acccgtggat
     2641 cgccgccgcg accgtgcgcc ggaccctccc cgggaccctc gacgtcttgg tccgggaacg
     2701 cggcccggac gccgcgatcc gcgccgggaa ggggtgggcc ctgctcgccg ccgacggcac
     2761 ggtcctcggc acgcgggacc ggcgcccgga cctgccgatg atcggggctc gcgtctccgg
     2821 cgcggcgccg ggcgcgcggc tcgtggggct gctcccgccg ctgcgggtgc tggcccacct
     2881 gcccgacgcg gtcccggccg tccgggcccg cccggacggg gagggcagcg tggtactggt
     2941 gctctccggt gggacgcgcg tccggtacgg cgaggcgcag ggctgggagc agaagaaccc
     3001 cgccatcgag gcgctgctcg cctgggcgga ccgccacggg gtggcggctg cccggatcga
     3061 cgtgcgggtg ccgcaatcgc cggcgctggc gccggccggg tgaccggagg ccggcgacgg
     3121 agccggcgcg aagggcactc cctcagcctg aaccggggcg tgacgtcacc cgctaaacct
     3181 cttgacggcg ggagccgctt cgtgtaaggt cctcgcctta cggtagaggt ttacataact
     3241 ctcaagctct agttgagctt gagaatgctt ccccagcatg cacgaaaccg caggttgggg
     3301 gccatggaca cggaggggta ggagagatgc agggtcccca gaactacctc gcggtgatca
     3361 aggtggtcgg gatcggcggc ggcggcgtga acgccgtgaa ccggatgatc gacgcgggcc
     3421 tgaagggtgt cgagttcatc gccatcaata ccgacgcgca ggccctcctg atgtccgagg
     3481 ccgacgtgaa gctggacatc ggacgggaga cgacccgcgg cctcgggtcg gggtcgaacc
     3541 cggacgtcgg cctccgcgcg gccgaggagc accgcaccga gatcgaggag atcctgaagg
     3601 gcgccgacat ggtcttcatc accgccggcg agggcggcgg gacggggacc ggcggggcgc
     3661 cgttggtggc cgagatcgcc cgggacatgg gcgccctcac gatcggcgtc gtcacccgcc
     3721 ccttctcgtt cgaggggcgc aagcgcgcca cccaggcgga catggggatc gccgacctga
     3781 agaaggccgt cgacacgctg atcatcgtgc cgaacgaccg tctgctccag gtgtcggacc
     3841 cgctgacgcc gatggtggac gcgttccgga tggccgacca ggtcctgtac cagggcgtgg
     3901 acggcatcac ctcg
//
LOCUS       DASPWO010000069         3058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_182150, whole genome shotgun sequence.
ACCESSION   DASPWO010000069 DASPWO010000000
VERSION     DASPWO010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182150"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..677
                     /locus_tag="VEO00_01605"
     CDS             150..677
                     /locus_tag="VEO00_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56731.1"
                     /translation="MARPATTLLLATLLAAGACSPAVQPPAGGAAPSATPADPIPQFG
                     ADGPSVRDCYSPRVRALGVPRQVPVPATAVVQGPDTYAGAYGSSADGALRRIQGTGFA
                     YEVGSPAGLVAGFHEACLQPYGPAGWRRLRGRAGDVLFRIPRPTGPDAVVLVAGAPIG
                     SLVVVFVEWRLTPGP"
     gene            complement(661..1872)
                     /gene="tyrS"
                     /locus_tag="VEO00_01610"
     CDS             complement(661..1872)
                     /gene="tyrS"
                     /locus_tag="VEO00_01610"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HYU56732.1"
                     /translation="MASLLRELLAGAAAVIPEGELEERLAAGRPLRVKLGIDPSRPDL
                     HLGHAVVLRKLRRFQDLGHTAVLIIGDFTGRVGDPSGQSETRSFLTAEEIEANARTYL
                     EQAGRVLDMDRAEVRRNSEWLAPLTFADVLGLAAQATVARMLERDDFADRYAAGRPIS
                     VVEFLYPLMQGYDSVAVAADVEMGGTDQTFNLLMGRDLQQRHGQPPQVVFTMPLLEGT
                     DGVRKMSKSFDNFVGLTEPPEEMFGKLMRVPDRLIPKYLLLCAGTALAEVERVERGLA
                     DGSVHPGQAKRAMAGAVVALYHGEEAATAAEARFDTVHVDHEIPADVPERAIPPEVVR
                     EGRVWLPRLLAALGLARSSSEVLRLLEQGGVRLDGEQVVDANREFALTELVGRVLQVG
                     RRRFVRLTGPA"
     gene            1969..>3058
                     /locus_tag="VEO00_01615"
     CDS             1969..>3058
                     /locus_tag="VEO00_01615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HYU56733.1"
                     /translation="MLRGAFPRSARLVALAVAVGLVAAGCGLPRLEDEQAKAPPLPQT
                     SIVYAADGTVLTTLHGEENRVAVPIARIPTELQHATVAIEDERFYDHRGVDLKAVMRA
                     LYVNAAEGRITEGGSTITQQYVKNTLIGNERTLDRKVREAALAYQLEEEMSKDEILEG
                     YLNTVYLGEGAYGVQAAAKAYFSRGVSGLDLRQSALLAGLISAPSDFDPLKHAKEARA
                     RRNVVLARMREQGYITSAEEQDARDRGLGLKPSDDDGRYPAPYFIDYVRRQIVEDPRF
                     GDTKAEREDLLYTGGLRIYTTLNRTYQRYAEEAVAEVLPFPDDPHAAMTVVDPRNGHI
                     VAMVGGRDYFARKDKVAKVNHATGDGGTG"
BASE COUNT          442 a         1140 c         1073 g          403 t
ORIGIN      
        1 agacgccctt cctcggccca cggggccgtt gtacaagggg atgatagcag cgcgctcgaa
       61 cgaccgtcaa tgcaccgaac atcgcgcgca gccaccggca cgttcgcgat cctgcccgtg
      121 gccgccgcct ccagtgtagc ctcgcggcga tggctcggcc ggccacgacg ctcctgctgg
      181 cgaccctgct cgccgcgggc gcgtgctccc cggccgtcca gcctcccgcc ggtggagcag
      241 ccccgtcggc gaccccggcc gacccgatcc cgcagttcgg ggcggacggt ccatccgtga
      301 gggactgcta ctcgccccgg gtccgcgcgc tgggggtccc gcgccaggtg cccgtcccgg
      361 ccaccgcggt cgtgcagggc cccgacacgt acgcgggcgc ctacggctcc tccgccgacg
      421 gcgcgctccg caggatccag gggaccggct tcgcctacga ggttggctcc cccgccggcc
      481 tggtggccgg cttccacgag gcctgcctgc agccgtacgg ccctgccggc tggcggcggc
      541 tgcgcggccg cgccggcgac gtactcttcc ggatcccccg gcccaccggt ccagacgctg
      601 tcgtcctcgt cgccggggcg ccgatcggct cgctggtggt cgtcttcgtg gagtggcgcc
      661 tcacgccggg cccgtgaggc ggacgaagcg ccgccgtccg acctgcagca cgcgcccaac
      721 caactccgtc aacgcaaatt cacgattggc atcaacaacc tgctcaccgt cgagccggac
      781 ccctccctgc tcaagcagcc gaaggacctc cgagctggac cgagccagcc cgagcgcggc
      841 cagcagacgc ggcagccaca cgcgcccctc gcggaccacc tcgggcggga tcgcccgctc
      901 ggggacgtcg gccgggatct cgtggtcgac gtggacggtg tcgaaccgcg cctcggcggc
      961 ggtggcggcc tcctcgccgt ggtacagcgc caccaccgcg ccagccatcg cccgcttggc
     1021 ctgccctggg tgcaccgacc cgtcggcgag cccccgctcg acccgctcga cctcagcgag
     1081 cgcggtcccg gcgcacagca gcaggtactt cgggatcagc cggtcgggga cgcgcatcag
     1141 cttgccgaac atctcctcgg gcggctcggt caggccgacg aagttgtcga aggacttgga
     1201 catcttgcgc acgccgtcgg tcccctcgag gagcggcatg gtgaacacca cctgcggggg
     1261 ctgcccgtgg cgctgctgca ggtcgcggcc catcagcagg ttgaacgtct gatcggtccc
     1321 gcccatctcg acgtcggccg ccaccgccac cgagtcgtac ccctgcatca gggggtagag
     1381 gaactcgacg acgctgatcg gccggccggc ggcgtagcgg tcggcgaagt cgtcgcgctc
     1441 gagcatgcgg gcaacggtcg cctgggccgc caggccgagg acgtcggcaa acgtcagcgg
     1501 ggcgagccac tccgagttcc ggcgcacctc ggcgcggtcc atgtcgagca cgcgcccggc
     1561 ctgctcgagg tacgtccggg cgttcgcctc gatctcctcc gcggtcagga acgagcgggt
     1621 ctcggactgc ccggaggggt ctccgacccg cccggtgaag tcgccgatga tgagcacggc
     1681 ggtgtgcccg aggtcctgga agcgccgcag cttgcgcagg accacggcgt gcccgaggtg
     1741 gaggtccggc cgggacgggt cgatcccgag cttgacccgc agtgggcggc cggcggccag
     1801 gcgctcctcc agttcgccct cggggatcac cgcagcggct ccggcgagga gctcgcgcag
     1861 gagggaggcc atggcgcgcg gcatcgtagc aggggcccgc cgggcaaccg gcagatcccg
     1921 tcgcaaaccc ccgagtttgg ccggggggcg tgggtatact cgcggggcat gctccgcggg
     1981 gctttccctc gatcggctcg gctggtcgcg ctcgcggtgg cggtgggctt ggtggccgcc
     2041 ggttgtggcc tgccccggct cgaggacgag caggcgaagg ccccgccgct cccccagacc
     2101 tcgatcgtct acgcggccga cggcacggtg ctcaccaccc tccacggcga ggagaaccgc
     2161 gtcgcggtcc cgatcgcccg cattccgacc gagctgcagc acgcgacggt ggccatcgag
     2221 gatgagcgct tctacgacca ccgcggcgtg gacctgaagg ccgtgatgcg cgccctgtac
     2281 gtgaacgccg ccgagggacg gatcaccgag ggcggctcga cgatcacgca gcagtacgtc
     2341 aagaacacgc tgatcggaaa cgagcgcacc ctggaccgca aggtccggga ggccgcgctc
     2401 gcgtaccagc tcgaggagga gatgtccaag gacgagatcc tcgaggggta cctcaacacg
     2461 gtgtacctcg gcgagggcgc gtacggcgtc caggccgccg ccaaggccta cttctcccgc
     2521 ggggtgtcgg ggctcgacct gcgccagtcc gcgctcctcg ccggcctgat ctcggcgccc
     2581 tcggacttcg atcccctgaa gcacgcgaag gaggcccgcg cccggcggaa cgtggtcctg
     2641 gcccggatgc gggagcaggg ctacatcacg tccgccgagg aacaggacgc ccgagaccgg
     2701 gggctcgggc tgaagccgtc cgacgacgac ggccgctacc cggcgcccta cttcatcgac
     2761 tacgtccggc gccagatcgt cgaggacccg cggttcggcg acacgaaagc cgagcgcgag
     2821 gatctcctct acaccggcgg gctgcgcatc tacacgaccc tcaaccggac ctaccagcgc
     2881 tacgccgaag aggcggttgc ggaggtcctc ccgttccccg acgaccccca cgcggcgatg
     2941 acggtggtcg atccgcggaa cggccacatc gttgccatgg tcggcgggcg ggactacttc
     3001 gcccggaagg acaaggtggc caaggtcaac cacgccaccg gcgacggggg aacggggc
//
LOCUS       DASPWO010000070         5389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_182244, whole genome shotgun sequence.
ACCESSION   DASPWO010000070 DASPWO010000000
VERSION     DASPWO010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182244"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..509)
                     /locus_tag="VEO00_01620"
     CDS             complement(135..509)
                     /locus_tag="VEO00_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYU56734.1"
                     /translation="MATIGDLATTELVSVPPSATVAEAATVMGERRCSSCLVMDGDQM
                     LGIFTERDIVRSLAQHFDAAGHSVSDWMTPNPTSVPSDTPVQEALQVMLDGGYRHLPI
                     VDGGKVMGVVSMRDISRWLGSA"
     gene            600..1547
                     /locus_tag="VEO00_01625"
     CDS             600..1547
                     /locus_tag="VEO00_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019617712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HYU56735.1"
                     /translation="MRGARALLEWYEPRRRSFPWRGAAPYAVLVSEVMLQQTQATRVL
                     RAYGQFLARFPTVDDLAAASPAAAIRTWDGLGYNRRAVALHRAARAIVERHGGRVPDS
                     VEALRALPGVGPYTAAAVACFAHGVQLPVVETNVRRVLARAALGVEPREVPPRDLEAI
                     AASSLPSGRAVDWNQALMDLGREVCRLRPRCPECALAPGCAYRRAGRTPAGAPNGGRV
                     WGGSSPRGESVSTTSGERTPQGKRQPRFEGSSRPARGRIVALLRDRGTLSLQGAAGEA
                     GLTTARAAVVVAGLARDGLVRAGPGAMRGHPRGRIGFPR"
     gene            1638..2537
                     /locus_tag="VEO00_01630"
     CDS             1638..2537
                     /locus_tag="VEO00_01630"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HYU56736.1"
                     /translation="MLARTGTGTRSARGIFVALLLVIGVAAAACGSGAGGSAGGGAVR
                     GPASPAPGEPGEPAGGEAPGRDLDVALAGPLPAIGARIVKTASLAVEVEQGAFSDRFE
                     RVSLIAGRFGGYVSSSSVSGTEERGGTIVIRVPAVSFERALADLRRLGEVTEEEISGE
                     DVTDQFVDLEARLRNWRAQEAVLLDLMERARTIRDTITVQNQLQQVQLTIEEIQGQLR
                     LLDDRTSLSTISIVLTEPGAAVPKPGEGPSIGRAWRDALDGFLAVIAAVIVGLGYAVP
                     LALMALLVWVVVRRQRPRPAAAG"
     gene            complement(2560..3594)
                     /locus_tag="VEO00_01635"
     CDS             complement(2560..3594)
                     /locus_tag="VEO00_01635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU56737.1"
                     /translation="MAVQIGLGLFTGQVPPGSGRPFAEEYRGVLELVREAEVLGFDSV
                     WVSEHHGAGDGYLPSLLAMLAAFAAVTGRVRLGTGVILAPLHAPLRLAEDAAVVDNLS
                     AGRLVLGLGRGWREEEFRMFGVPPGHRLSRTIEPVEILRRAFTGQRFSFEGKVFRYED
                     VLVTPPPARPGGPPIYLGGFEERTIRRAGAIGDGYIRSRSGLQDSLQALRWAEEGARE
                     AGKDPEALGFTLLQNAFAWDEGDAWEPVRAGVEHQIATYAGWREASDTPGHGFENPPV
                     DEQMLKHLTIHGTPDEVLHGLRPIVDAFAGRPEFTLIVRLQYPGMDDDTARRAVELFG
                     TEVLPALKGA"
     gene            3671..4150
                     /locus_tag="VEO00_01640"
     CDS             3671..4150
                     /locus_tag="VEO00_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HYU56738.1"
                     /translation="MPVWPSEEWSTEYAKLINASSEYREAAHDWEGVVVYHFQAEPDK
                     GLGGDVYGWFDLWHGECREAKQVTAEEAAKAEFLVSASYSTWKQVLLKQLDPMKALMQ
                     GRLKMRGDLPKAVRYTKATMILNDLATQVADTGFLDELPKERLQELADAGQPVVVPD"
     gene            4195..4848
                     /locus_tag="VEO00_01645"
     CDS             4195..4848
                     /locus_tag="VEO00_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYU56739.1"
                     /translation="MTVAVTGPTGDIGRPFVGALEREPRLGRVVGMARSAVDPTEDGW
                     TKTEYVRGDILDRAPVHRLVEGVDVVVHLAFVIMGSDADTRRINVDGSRSVFEAAVAS
                     HEDVMAAALVAAMLGNGPPGVYNVAGPGRLTLTDLANAFGWKSVKVPAAAARFTAAAV
                     TRLPFLPPKLDWVNALRGMTLMDTAKAQRELGWKPTHDAHETLSQLVESARERGLLR"
     gene            complement(4849..>5389)
                     /locus_tag="VEO00_01650"
     CDS             complement(4849..>5389)
                     /locus_tag="VEO00_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU56740.1"
                     /translation="PPDLAAFIEAGPPPVAIGYGSMGDRDPETLTGISIEALRLAGQR
                     GVLVSGWAGLKKEDLPDHVAAIEAIPHEWLFPRMAAAVHHAGPMTLAVALRAGIPGVS
                     VPFFFDQLWWADRAAALGIGPKAVPRRTLTAESLAQAIDRAVTDDRIRKTVAEIGEQV
                     RDEDGIATAVEAVARVTGS"
BASE COUNT          746 a         1926 c         2027 g          690 t
ORIGIN      
        1 ggcccagagc gcccggaacg ggcgacgaca ccggcccctg agaatgagaa tgatcctcac
       61 tctgacgcgg aaccctatcc gaggcgagcc ggagcgtcaa gcggacgggt cggggtaggg
      121 atcgcggcag acggctacgc ggagccgagc cagcgggaga tgtcacgcat cgagacgacg
      181 cccatcacct ttccgccgtc cacgatcggc aggtggcgat agccgccgtc gagcatcacc
      241 tgcagcgcct cctggacggg ggtgtcggac ggcaccgagg tgggattggg ggtcatccag
      301 tcggagacgg agtggccggc ggcgtcgaag tgctgcgcga gcgaccgcac gatgtcacgc
      361 tcggtgaaga tcccgagcat ctgatcgccg tccatcacca ggcacgacga gcagcgacgc
      421 tcgcccatca cggtggcggc ctcggccacg gtcgccgacg gcggcacgct gaccagctcg
      481 gtcgtcgcca ggtcgccgat cgtcgccacg aggcaagcat accgaaaggg tgccccgcga
      541 tcgaaaccga gcaccggtcg gcgggacgcg gcgagcgccc gaccgctacg ctgcgggacg
      601 tgcgcggcgc gcgggcgctc ctcgagtggt acgaaccccg ccggcgctcg ttcccgtggc
      661 gcggcgccgc cccctacgcg gtcctcgtct ccgaggtgat gctgcagcag acgcaagcca
      721 cgcgcgtcct ccgcgcatac gggcagttcc tggcacggtt cccgacggtc gacgaccttg
      781 cggccgcctc gccggcggcc gccatccgga cgtgggacgg cctcgggtac aaccggcggg
      841 ccgtcgcgct gcaccgcgcc gcacgggcga tcgtcgagcg ccacggtggc cgcgtgccgg
      901 actcggtgga ggcccttcgg gcgctccccg gcgtcgggcc ctacacggcg gcggccgtgg
      961 cgtgcttcgc ccatggggtg cagctgccgg tggtcgagac gaacgtccgg cgtgtcctgg
     1021 cccgggcggc actcggggtc gagccgcgcg aggtaccccc tcgcgacctg gaggcgatcg
     1081 ccgcgagctc cctgccgtcc ggccgggcgg tcgactggaa ccaggcgctg atggacctcg
     1141 gccgcgaggt ctgccggttg cgtccgcggt gtcccgagtg tgcgctggcg cccggctgcg
     1201 cctaccggcg ggccggccgg acgccagcgg gcgctccgaa cggcgggagg gtttgggggg
     1261 gtagctcgcc ccggggagag agcgtctcca ccacgtccgg cgagcgaacc ccccaaggga
     1321 aacggcagcc acggttcgag ggctcgtcgc gcccggcgcg tggcaggatc gtcgccctcc
     1381 tgcgggaccg cggaaccctg tcgctacagg gggcggccgg tgaggccggg ctgaccacgg
     1441 cgcgcgccgc cgtggtcgtg gccgggctgg cgcgcgacgg cctggtccgc gccgggcccg
     1501 gcgccatgcg cggccacccg cgcggccgga tcggctttcc tcgctgacgc tcgcgcgagc
     1561 gtttccacgc gatcctgcgg gaaccacgcg cccactgcgc ccgtcccacg tacaaccaag
     1621 gcgaggaggt tgtccggatg ctcgctcgta ccggaaccgg cacgcgttcc gcccgcggga
     1681 tcttcgtcgc gctcctcctg gtgatcggcg tcgccgcggc tgcctgcggg agtggtgcag
     1741 gggggtcggc gggcggcggc gcggtgcgcg gcccggccag cccggcgccg ggggagcccg
     1801 gcgagcccgc cggtggggag gcgccaggcc gggacctcga cgtcgcgctc gccggaccgc
     1861 ttccggcgat cggcgcgcgg atcgtcaaga ccgcgtccct ggccgtcgag gtagagcagg
     1921 gcgccttcag cgaccgcttc gagcgggttt ctctgatcgc cggccgcttc ggcggctacg
     1981 tctcgtcgtc gtcggtctcg ggcacggagg agcgcggcgg cacgatcgtg attcgggtac
     2041 cggcggtgtc cttcgagcgc gcgctggccg acctgcggcg gctcggtgag gtgaccgagg
     2101 aggagatctc gggcgaggac gtcaccgacc agttcgtcga cctcgaggcg cggctgcgga
     2161 actggcgcgc gcaggaggcg gtcctgctcg acctgatgga gcgggcgcgg acgatccggg
     2221 acaccatcac ggtccagaac cagctgcagc aggtgcagct gacgatcgag gagatccagg
     2281 ggcagctccg gctgctcgac gaccggacct cgctctcgac gatctcgatc gtgctgaccg
     2341 agcccggcgc cgccgtcccg aagcccggcg agggaccctc gatcgggagg gcctggcgcg
     2401 acgcgctcga cggtttcctc gccgtgatcg ccgccgtgat cgtcgggctt ggctacgcgg
     2461 tgccgctcgc gctgatggcg ctcctggtgt gggtggtggt gcgccggcag cggccgcggc
     2521 cggccgccgc gggctgacgc gggagacggg aggcggggct caggcgccct tcagggcggg
     2581 gaggacctcg gtcccgaaca gctcgacggc gcggcgcgcg gtgtcgtcgt ccatgccggg
     2641 atactgcagc cggacgatca gcgtgaactc cggccggccg gcgaacgcgt ccacgatcgg
     2701 gcgcaggcca tggaggacct cgtcgggcgt cccgtggatc gtcaggtgct tcagcatctg
     2761 ctcgtcgacc ggcgggttct cgaacccgtg gccgggggtg tcggatgcct cgcgccaccc
     2821 ggcgtacgtc gcgatctgat gctcgacgcc ggcgcgcacc ggctcccagg cgtcgccctc
     2881 gtcccaggcg aacgcgttct gcagcagggt gaaccccagc gcctcggggt ccttcccggc
     2941 ctcgcgcgcc ccctcctcgg cccagcgcag tgcctggagc gaatcctgca gcccggaccg
     3001 gctgcggatg tagccgtcgc cgatcgcgcc cgcccggcgg atcgtccgct cctcgaaccc
     3061 gcccagatag atcggcggcc caccgggacg cgccggcgga ggcgtcacca ggacgtcctc
     3121 gtagcggaag accttcccct cgaacgagaa tcgctggccg gtgaaggccc ggcgcaggat
     3181 ctccacgggc tcgatcgtcc gtgacagccg gtgcccgggc gggaccccga acatccggaa
     3241 ctcctcctcg cgccagccgc gcccgaggcc gagcaccagc cggcccgcgg agaggttgtc
     3301 gaccaccgcg gcgtcctcgg ccaggcggag tggggcgtgg agcggagcga ggatcacgcc
     3361 ggtcccgagc cgcacccggc cggtgaccgc cgcgaacgcc gccagcatcg ccaggagcga
     3421 ggggagatag ccgtcgccgg cgccgtggtg ctcgctgacc cacaccgagt cgaagccgag
     3481 gacctcggcc tcccggacca gctcgagcac gccacggtac tcctcggcga acgggcgtcc
     3541 cgatcccggg ggcacctgtc cggtgaacag ccccagcccg atctggacgg ccacgcgcga
     3601 acgctagcag aatggccgcg cacgcccgac accgaacgga gcccgatcga cgaacgagga
     3661 ggggagcgcg atgccggtgt ggcccagcga ggagtggtcg accgagtacg cgaaactgat
     3721 caacgccagc tccgaatacc gcgaggcggc gcacgactgg gagggcgtgg tcgtctacca
     3781 cttccaggcc gagccggaca aggggctcgg cggggacgtc tacggctggt tcgacctgtg
     3841 gcacggcgag tgccgggagg cgaagcaggt cacggcggaa gaggcggcga aggccgagtt
     3901 cctggtctcg gcctcgtact ccacgtggaa gcaggtgctg ctcaagcagc tcgacccgat
     3961 gaaggcgctg atgcagggcc ggctgaagat gcgcggcgac ctgccgaagg ccgtgcgcta
     4021 cacgaaggcg acgatgatcc tgaacgatct ggcgacgcag gtggccgaca ccgggttcct
     4081 ggacgagctg ccgaaggagc ggctgcagga gctggccgac gcgggccagc cggtcgtcgt
     4141 ccccgactga cggtggcggc gccgcggcgg gaaccgcgcg aggacgtccg cggcatgacc
     4201 gtcgccgtca ccggcccgac cggggacatc gggcgtccgt tcgtcggggc cctggaacgc
     4261 gagccgcgcc tcgggcgcgt cgtcggcatg gcccgtagcg cggtcgatcc cacggaggac
     4321 ggctggacga agaccgagta cgtgcgcggt gacatcctcg accgcgcgcc ggtccaccgc
     4381 ctggtcgagg gcgtcgacgt ggtggtgcac ctggcgttcg tgatcatggg gtctgacgcg
     4441 gacacgcggc ggatcaacgt ggacggctcg cgctccgtgt tcgaggccgc agtggcgagc
     4501 cacgaggacg tcatggccgc cgccctggtg gcggcgatgc tcgggaatgg tccgcccggc
     4561 gtctacaacg tcgccggccc cgggcggctg acgctgaccg atctggcgaa cgccttcggc
     4621 tggaagtcgg tgaaggtccc ggccgccgcc gcccgcttca ccgcggcggc ggtgacccgc
     4681 ctcccgttcc tcccgccgaa gctcgactgg gtcaacgccc tccgcggcat gaccctgatg
     4741 gacaccgcca aggcgcagcg cgagctgggc tggaagccga cccacgacgc ccacgaaacc
     4801 ctgtcccaac tcgtcgagag cgcccgagag cgcggcctgc tgagatagtc aggagccggt
     4861 gacgcgggcg acggcctcga cggcggtggc gatgccgtcc tcgtcgcgaa cctgctcgcc
     4921 gatctcggcg accgtcttcc ggatccggtc gtccgtgacg gcccggtcga tcgcctgcgc
     4981 gagcgattcc gccgtgagcg ttcggcgagg aacggccttc gggccgatgc cgagggcggc
     5041 ggcgcggtcg gcccaccaga gctggtcgaa gaagaacggg accgacacgc cggggatgcc
     5101 ggcgcgcagc gcgacggcga gcgtcatcgg gcctgcgtgg tgcacggccg ccgccatccg
     5161 cgggaacagc cactcgtgcg ggatcgcctc gatcgccgcc acgtggtccg gcaggtcctc
     5221 cttcttcagc cccgcccagc cgctgaccag caccccgcgc tggccggcca ggcgcagcgc
     5281 ctcgatcgag atcccggtca gcgtctccgg gtcgcggtcg cccatgctgc cgtagccgat
     5341 cgccaccggc ggcggcccgg cctcgatgaa cgcggccagg tcggggggc
//
LOCUS       DASPWO010000071         2909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_94598, whole genome shotgun sequence.
ACCESSION   DASPWO010000071 DASPWO010000000
VERSION     DASPWO010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94598"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VEO00_01655"
     CDS             complement(<1..625)
                     /locus_tag="VEO00_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56741.1"
                     /translation="MSLSALVLLSVVVLVPGLGATLALWRPGEVGLPTRMALAAGFGY
                     AIWALIAFALAMLHALRAPVLVPLMAAATAGLWWFGLRRGSLAAHVRAVRDEVRADPW
                     TMVAGLLVLIGLAVVRGTYSSLLDLRGASPFRYWADGLEIADGGRIPDLTLQWGMAMV
                     PTVSKVMLNAFNASASFVLGTNALHALGPLLWLSSVGLATAFWALGRE"
     gene            691..2703
                     /locus_tag="VEO00_01660"
     CDS             691..2703
                     /locus_tag="VEO00_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56742.1"
                     /translation="MKASARAAPESPPTRAEPQPGTERGWIPSAAAIVGVAAAIVAFF
                     LAIYLAQRLTVPLGWDTSSYLWRTALVKARGLGALHESLPPPVRTFPDRPGFPLVAGA
                     LSALGGVSPVRLAAVIPALSCAAIGLAAGALVSHVLRRPPWECAVVAGAVATSAAVVR
                     LAAPEAYQDNLFGAAILLAAAVPLAMAARGDPRGVLPGAVLLGAAGAVHWATFLLFAA
                     AVLVVAVATVPRSLALRRSGVPTIRTPAALLAAALAAGAVLAAFVVYVLLGTRPQGPS
                     LSPSEFSKKLRQDLPRYRLWATLPLAALGAVALWSSIRRRSPQDGERDGSRFLLWLLV
                     AWSAVVLAGIAAYALGKPVPAHRFLAFALAVPILGAIGVLWAARRSARAVRRGPRARA
                     LAAGAVIVVGLVPLTVLSQRDWLRQPDYARMDHAKVLEAATALAYAERLDRPEDAAIV
                     FVIDDRGPNPGATASLIGHMLRVAQTPDQFDDVYLYVGTPENFLAGRPTTSDTAPTYD
                     AVSRQYLRALRPRLAERPLAFVTKSSNQHDYFAWEIAHPESRVAEGIALVQGGPPPAQ
                     DAEAPNLPLAVYPPLYLTLLAGGAFAVLFLAGLGWILALSGGRLRRHEALALSPAAGI
                     AALVVGGLIADRLGVRLTGVGGASVPVAIAALGWAALWLRHRRKAT"
     gene            complement(2704..>2909)
                     /locus_tag="VEO00_01665"
     CDS             complement(2704..>2909)
                     /locus_tag="VEO00_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015937680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HYU56743.1"
                     /translation="LEYVRHVAARADRPWFAIGGIDPGNVAEVVVAGATRAVVVRAVT
                     EAADPAAAARVLRAALEGAPTRR"
BASE COUNT          313 a         1120 c         1087 g          389 t
ORIGIN      
        1 gctcgcggcc gagggcccag aacgccgtgg cgagcccgac cgacgacagc cacagcaacg
       61 gtccgagggc gtgcagcgcg ttcgtgccca ggacgaagct cgcgctggcg ttgaaggcgt
      121 tcagcatgac cttgctgacc gtggggacca tggccatccc ccactgcaag gtcaggtcgg
      181 ggatgcggcc gccgtcggcg atctccaggc cgtcggccca gtaccggaac gggctcgccc
      241 cgcgcaggtc gaggagggac gagtaggtcc cgcggacgac cgccagcccg atgagaacca
      301 gcagcccggc caccatcgtc cacgggtcgg cgcgcacctc gtcgcgcacc gcccggacat
      361 gggcggcgag gctcccacgc cgcaggccga accaccacaa tcccgccgtc gcggccgcca
      421 tgaggggaac gaggacgggc gcgcgcagcg cgtggagcat cgccaacgcg aacgcgatca
      481 gagcccagat cgcgtacccg aagccagcgg cgagggccat gcgcgtcgga agccccacct
      541 cgcccggccg ccagagggcg agcgtcgccc cgagcccggg gaccagcacg acgacggaga
      601 gcaggacgag cgcggagagg ctcacgcggc cgcgattgtc ccatgcctct ttgtgccggc
      661 tccgccggct ccgctggtac ggttgcctgc gtgaaggcct ccgctcgggc cgcgccggag
      721 tcgccgccca cgagagccga accccagccc gggaccgagc gcggctggat cccgtcggcg
      781 gcggcgatcg tcggggtggc cgccgcgatc gtcgccttct tcctggcgat ctacctcgcc
      841 cagcgcctga cggtgccgct cggctgggac acgtccagct atctgtggcg caccgcgctc
      901 gtcaaggcgc gtggcctcgg ggcgctgcac gagtcgctgc ccccgccggt gcgcaccttc
      961 cccgaccggc ccggcttccc gctcgtcgcc ggcgcgctct cggcgctcgg cggcgtttcc
     1021 cccgttcggc tcgccgcggt gatccctgct ctcagctgcg cggcgatcgg tctggcggcg
     1081 ggggcgctcg tctcccacgt cctgcggcgg ccgccctggg agtgcgcggt cgtcgcgggg
     1141 gccgtcgcca cctcggcggc cgtggtccgg ctcgcggcgc cggaggccta ccaggacaac
     1201 ctgttcggcg cggcgatcct gctcgccgcg gccgtccccc tggcgatggc ggcccgcggc
     1261 gatccccggg gcgtgctgcc cggcgcggtg ctgctcggcg cggccggggc ggtgcactgg
     1321 gcgacgttcc tgctgttcgc ggcggcggtc ctcgtcgtcg cggtggccac ggtcccccgc
     1381 tcgctcgcgc tccggcggtc cggcgtcccc acgatccgta ctccggcggc cctgctggcg
     1441 gccgcgctgg ctgctggggc ggtgctcgct gcgttcgtgg tctacgtcct gctcgggacg
     1501 cggccgcagg gcccgagcct ctcgccgtcg gagttctcga agaagctgcg ccaggacctc
     1561 ccgcggtacc ggctgtgggc cacgctcccc ctcgccgcgc tcggggcggt ggcgctgtgg
     1621 agctcgatcc gtcggcggtc gccgcaggac ggggagcggg atgggtcgcg gttcctgctc
     1681 tggctcctgg tggcgtggag cgcggtcgtc ctggcgggga tcgccgccta cgcgctcgga
     1741 aagccggtgc cggcgcaccg gttcctcgcc ttcgcgctgg ccgtcccgat cctcggcgcg
     1801 atcggcgtgc tgtgggccgc ccggcgctcc gcccgcgcgg tgcgccgggg cccgcgggcc
     1861 cgcgcgctcg cggccggcgc ggtgatcgtg gtgggcctgg tcccgctcac cgtcctgtcg
     1921 cagcgcgact ggcttcgcca gccggactac gcgcggatgg accacgcgaa ggtcctcgag
     1981 gcggcgaccg cgctggccta cgccgagcgg ctggaccggc ccgaggacgc ggcgatcgtg
     2041 ttcgtcatcg acgaccgggg cccgaacccg ggcgccaccg cgtcgctgat cgggcacatg
     2101 ctgcgggtgg cgcagacgcc ggaccagttc gacgacgtgt acctctacgt cggcaccccg
     2161 gagaacttcc tcgccgggcg gccgaccacg tcggacaccg cgccgacgta cgacgccgtg
     2221 tcgcgtcagt acctgcgcgc gctgcggccg cggctcgcgg agcggccgct cgcgttcgtc
     2281 accaagtcgt cgaaccagca cgactacttc gcctgggaga tcgcgcaccc ggagtcgcgc
     2341 gttgcggagg gcatcgcgct ggtgcagggc ggcccgccgc cggcgcagga cgcggaagcg
     2401 ccgaacctcc cgctggcggt ctacccgccc ctgtacctga cgctcctggc cggcggggcg
     2461 ttcgccgtgc tgttcctggc cgggctgggg tggatcctgg cgctctccgg cggccgcctg
     2521 cgccggcacg aagccctggc gctctcgccg gcggccggca tcgccgcgct cgtggtgggc
     2581 ggcctcatcg ccgaccggct gggggtgcgc ctgacgggcg tcggaggagc atcggtcccg
     2641 gtggccatcg cggcgctcgg ctgggccgcg ctgtggctgc gccaccggcg gaaagccacc
     2701 tgactatcgg cgggttggag cgccctccag cgccgctcgc agcactcggg cggcggcggc
     2761 cgggtcggcc gcctcggtga cggcccggac caccacggcg cgggtcgccc cggcaaccac
     2821 cacctcggcg acgttccccg gatcgatgcc gccgatggcg aaccacggcc ggtcggcccg
     2881 cgccgccacg tgccgcacgt actccagcc
//
LOCUS       DASPWO010000072         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_522858, whole genome shotgun sequence.
ACCESSION   DASPWO010000072 DASPWO010000000
VERSION     DASPWO010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522858"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="VEO00_01670"
     CDS             complement(<1..1628)
                     /locus_tag="VEO00_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYU56744.1"
                     /translation="MLDEADVRAPGPNHVELQGMDVGGVLETAIFQHPESEVRFSLPE
                     GFTGGTLRLGYGIKTRAWEWMRGAVAFRVGVTGAGGGSDAEPEWLLESTLEPRSRPEH
                     RSWERAVVAVPSGGTLVLRTEAVGSADHAWSGWADPLLEWEEPARAARPTRAAHPNLL
                     LITSDACRADYLSCYGSAEVRTPNLDALAAEGLRFLDARANATTTVGSYASLLTGRVP
                     PSHGAIADWGALPAGIPTLAGALANAGYRTVFAPSSGEVGIPQRGFTGAFERTIPSLG
                     NPVQDGGVTTRRLLRDLAGSTDGPWFAWVCYRETHPPTRLPDRLVRPFYEGDPRDPAR
                     AWRADDVEAIRASESLLYLDQALPLLRRGHVAGDLYWRLLGTAAALDDAAQPPPDLAW
                     HLRALGDGARRGRSEAVFARWLRKRTRWLERGRIDPELVHWLETVADRLRDVERDARD
                     WLDGVVDYRYPLAQLRAGAAHLDRQVGALVAHLREEGLYESSTIVFCSPHGELLGEGG
                     ANFHHAEPREPVLRIPLVAKLGREAGRRRAEVDGS"
     gene            complement(1652..>2667)
                     /locus_tag="VEO00_01675"
     CDS             complement(1652..>2667)
                     /locus_tag="VEO00_01675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU56745.1"
                     /translation="EHDNPFVGARVPRLLAEWRPDIAHVTHGMKLTVAAMDACRALGI
                     PAVVSLTDYWFLCPRHTLLTWDGRLCDGPSEWRECFRCLQDLHGVATRRRERRAVQGR
                     PDRTRMAVESAARVFALSSFLKRMYVANGYDPERIEVLPHGIETEGLHPAGPRRPGPT
                     RFLFVGSLQPFKGAHVPVEALAADPGLDVELVLHGAGGPAEYAERLARSAGGDARIVL
                     AGRFEPGDLPRILGGTDYVLIPALWYENEPIIVKAAKHIGIPVLASRIGSLVEMIEEG
                     LDGWTVPPGDVAAWLEAMRRAVLERDRWQRVPRPQPSMDDQFAYLDGVYAELAAARSA
                     VRT"
BASE COUNT          367 a          989 c          971 g          340 t
ORIGIN      
        1 gacccgtcga cctccgcccg cctccgcccg gcctcacggc ccagcttggc gacgaggggg
       61 atccgcagca ccggctcccg tggctccgcg tgatggaagt tcgcgccccc ctccccgagg
      121 agctcgccgt ggggagagca gaagacgatc gtgctgctct cgtagagccc ctcctcgcgc
      181 agatgggcga ccagggcgcc gacttgtcgg tcgaggtgcg ctgcgccggc tctcaactgc
      241 gccagcgggt agcggtagtc caccaccccg tcgagccagt cacgggcgtc gcgctccacg
      301 tcccgcagcc ggtcggcgac ggtctcgagc cagtgcacga gctcggggtc gatcctcccg
      361 cgctcgagcc agcgcgtccg cttgcggagc caccgggcga agaccgcctc cgaccggccg
      421 cgccgcgcgc cgtcgcccag cgcgcgcagg tgccaggcga ggtccggggg cggttgcgcc
      481 gcgtcgtcca gcgccgcggc cgtgccgagc aggcgccagt acaggtcgcc ggccacgtga
      541 ccccggcgca ggagcggaag ggcctggtcc aggtagagca gtgactcgga cgccctgatg
      601 gcctcgacgt cgtccgcgcg ccacgcccgc gccgggtcgc ggggatcccc ctcgtagaag
      661 ggccgaacca ggcggtcggg caagcgggtg gggggatggg tctcccggta gcagacccag
      721 gcgaaccacg gcccgtcggt cgacccggcc aggtcgcgca ggagccggcg ggtggtcacg
      781 ccgccgtcct gaacggggtt cccgagtgag gggatcgtgc gctcgaacgc gccggtgaac
      841 cctcgctgcg ggatcccgac ctcccccgag ctcggggcga acacggtccg gtatcccgcg
      901 ttcgccagcg cgcccgccag ggtcgggatc ccggcgggca gggctcccca gtccgcgatc
      961 gcgccgtgcg agggcggaac ccgaccggtg agcagcgagg cgtagctccc gaccgtcgtg
     1021 gtcgcgttgg cccgggcgtc gaggaaccgc aacccctccg ccgccagtgc atcgaggttc
     1081 ggcgtgcgca cctcggcgct cccgtagcac gacaggtagt ccgcccggca cgcgtcgctc
     1141 gtgatgagca gcaggttggg atgggcggct cgcgtcgggc gcgccgcccg ggcgggttcc
     1201 tcccactcca ggagcgggtc ggcccacccc gaccaggcgt gatcggccga cccgaccgct
     1261 tcggtccgca gcaccagggt cccgccggac gggaccgcca ccacggcgcg ttcccaggag
     1321 cggtgctccg gacgggagcg cggctcgagg gtggactcga gcagccattc gggctccgcg
     1381 tcggagcctc cgccggcgcc ggtgaccccg acgcggaagg cgacggctcc cctcatccac
     1441 tcccaggcgc gggtcttgat cccgtacccg agccggagcg tgcctcccgt gaatccctcc
     1501 gggagggaga agcggacctc ggattccgga tgctggaaga tggccgtctc gaggacgccg
     1561 ccgacgtcca tcccctgcag ctccacgtgg ttcggcccgg gcgcccggac gtccgcctcg
     1621 tccagcacgt cggtcagatg gagggttcgc ctcacgtgcg gaccgcggaa cgggccgcgg
     1681 cgagctccgc gtagacgccg tcgaggtagg cgaactggtc gtccatgctc ggctgcgggc
     1741 gcgggacccg ctgccagcga tcgcgctcga ggacggcgcg gcgcatcgcc tccagccatg
     1801 ccgccacgtc acccggaggg acggtccacc cgtccagccc ctcctcgatc atctcgacga
     1861 ggctcccgat gcggctggcc agcacgggga tcccgatgtg cttggcggcc ttcacgatga
     1921 tcggctcgtt ctcgtaccag agtgccggga tcagaacgta gtccgtgccg ccgaggatcc
     1981 gcggcagatc gcccggctcg aagcggccgg ccagcacgat ccgcgcgtcg ccgcccgcgg
     2041 accgggccag gcgctcggcg tactcggcag gtcctcccgc gccgtggagc accagctcaa
     2101 catcgaggcc aggatcagcg gcgagcgcct cgaccggcac gtgcgcgccc ttgaagggtt
     2161 gcaggctgcc cacgaacagg aaccgggtcg ggcccggacg gcggggtccg gccgggtgaa
     2221 gcccctcggt ctcgatgccg tgaggcagga cctcgatccg ctccgggtcg tacccgttgg
     2281 cgacgtacat ccgcttcagg aacgacgaga gcgcaaagac gcgagcggcg gactcgacgg
     2341 ccatccgcgt gcggtccggt cgaccctgga cggcccgtcg ctcgcgccgg cgcgtggcca
     2401 cgccgtgcag gtcctgcagg caccggaagc attcccgcca ctcgctcggc ccgtcgcaga
     2461 gccgaccgtc ccaggtcagc agcgtgtgcc ggggacagag gaaccagtag tcggtcaggg
     2521 agacgacggc cgggatgccg agcgctcggc acgcgtccat cgccgcgacc gtcagcttca
     2581 tcccgtgcgt gacgtgggcg atgtcgggac gccattcggc cagcaggcga gggacgcggg
     2641 ccccgacgaa cgggttgtcg tgctcgt
//
LOCUS       DASPWO010000073         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1033480, whole genome shotgun sequence.
ACCESSION   DASPWO010000073 DASPWO010000000
VERSION     DASPWO010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033480"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /locus_tag="VEO00_01680"
     CDS             <1..1574
                     /locus_tag="VEO00_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HYU56746.1"
                     /translation="GQPTVILAKTVKGWTIGSFEGRNATHQMKKLSKQDLKDFRDRLY
                     LDIPDSALEAPLPPYYHPGESSDEIQYMKERREALGGHLPHRVVRAKPLKLPGDPVYA
                     DLMKGSGKQAVATTMAFVRLLKDLMKDKEIGARFVPIIPDEARTFGMDAMFPTAKIYS
                     PWGQLYEAVDRELLLSYKESPQGQILHEGISEAGAMGSVIASGTSYSTHGEHTIPIYI
                     FYSMFGFQRTGDAIWALADQLGRGFLLVATAGRTTLNGEGLQHEDGHSQLLASTNPAC
                     VSYDPAWAYEISFIVKDALHRMYGSSEKYPHGEDIFYYLTVYNEPYLQPAVPDIPGLE
                     EKVLRGLYRYQQAPEGNGDRPRAQILASGPAIQWALEAQRLLAEDWGVAADVWSATSW
                     TELRREAMECDKQNLLHPESEQRVPYVTQALEGVPGPVVAVSDWMRAVPDQIAQWVPA
                     DYASLGTDGFGRSDTRGALRRFFKVDAHSITVAVLTELVRRGEVKREAPAEAINQYQL
                     LDVQAAPAGNTGGET"
     gene            complement(1693..>2345)
                     /locus_tag="VEO00_01685"
     CDS             complement(1693..>2345)
                     /locus_tag="VEO00_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56747.1"
                     /translation="VYLEVGRKRVLACALDWPGWCRSGKNEELALEALQAYIPRYSIV
                     ARQAGVAFPVTAGDVSYAVVERLPGTATTVFGAPGEWAASDAQPLTSAEAERHAALVR
                     ASWAVFDGVVAEAPAELRKGPRGGGRDRDKMVDHVLGAEAAYARKPGVKHRQPAIDDA
                     VAIADLRDAIIGVLSAPSDGTPLVPKGWPARYAARRIAWHVLDHAWEIQDRSDPAG"
BASE COUNT          371 a          804 c          847 g          323 t
ORIGIN      
        1 ccgggcagcc cacggtgatc ctggccaaga cggtcaaggg ctggacgatc ggctcgttcg
       61 agggccgtaa cgccacccac cagatgaaga agctgtccaa gcaggatctc aaggacttcc
      121 gggaccggct gtacctggac atccccgact cggcgctgga ggccccgctg ccgccgtact
      181 accaccctgg ggagagctcg gacgagatcc agtacatgaa ggagcggcgg gaggccctgg
      241 gcggacacct gccgcaccgc gtggtgcggg ccaagccgct caagctgccc ggcgaccccg
      301 tgtacgcgga tctcatgaag ggctcgggca agcaggcggt cgcgaccacg atggcgttcg
      361 tgcggctgct caaggacctc atgaaggaca aggagatcgg cgcccgcttc gtgccgatca
      421 tcccggacga ggcccggacc ttcggcatgg acgcgatgtt ccccacggcg aagatctact
      481 cgccgtgggg gcagctgtac gaggccgtgg accgggagct gctgctctcc tacaaggagt
      541 ccccgcaggg gcagatcctg cacgagggca tcagcgaggc cggcgccatg ggatcggtga
      601 tcgcgtcggg cacgtcgtac tccacgcacg gcgagcacac gatcccgatc tacatcttct
      661 actcgatgtt cggcttccag cggaccggcg acgctatctg ggcgctcgcc gaccagctgg
      721 gccggggctt cctgctggtt gccaccgcgg ggcgtaccac gctcaacggc gaggggctcc
      781 agcacgagga cgggcactcg cagctgctgg cctcgaccaa cccggcctgc gtgtcctacg
      841 acccggcgtg ggcgtacgag atcagcttca tcgtgaagga cgcgctgcac cggatgtacg
      901 ggagcagcga gaagtacccg cacggcgagg acatcttcta ctacctcacc gtctacaacg
      961 agccgtacct gcagccggcc gtgccggaca tccccggcct cgaggaaaag gtgctcaggg
     1021 gcctgtaccg gtaccagcag gcccccgagg ggaacggcga ccggccgcgg gctcagatcc
     1081 tggcctcggg tccggcgatc cagtgggcgc tcgaggccca gcggctgctc gcggaggact
     1141 ggggggtggc ggccgacgtg tggtcggcca cgtcctggac cgagctgcgc cgcgaggcca
     1201 tggagtgtga caagcagaac ctgctgcacc ccgagtcgga gcagcgggtg ccgtacgtga
     1261 cgcaggcgct ggagggcgtg cctggcccgg tggtggccgt atccgactgg atgcgggcgg
     1321 tgcctgacca gatcgcgcag tgggtgccgg ccgactatgc ctcgctcggc acggacggct
     1381 tcggccggtc ggacactcgg ggagcgctac gccggttctt caaggtggac gcgcattcga
     1441 tcaccgtggc ggtgcttacc gagctggtac gccgcggcga ggtcaagcgg gaggcgcccg
     1501 ccgaggcgat caaccagtac caactgctcg acgtgcaggc ggcaccggcg ggcaacaccg
     1561 gcggggagac ctgaccgacg acgtgcaagg ggccccgagc ttcctcgggg cccccttgcg
     1621 cgcctgtcag cccgcggcca gcacgctctg gcgcgcgacc gcctggggcg gcacaggcag
     1681 ggtgccgcgg cgtcagcccg ccgggtcgct gcggtcctgg atctcccagg catggtcgag
     1741 gacgtgccag gcgatgcggc gggcggcgta gcgcgccggc cagcccttcg ggacgagcgg
     1801 cgttccgtcc gagggcgcgc tgagcacgcc gatgatggcg tcgcggaggt cggcgatggc
     1861 gacagcgtca tcgatggcgg gctggcggtg cttcacgccg ggctttcgcg cataggcggc
     1921 ctccgcgcct agtacatggt cgaccatctt gtcgcgatcg cggccgccgc cgcggggacc
     1981 cttgcgcagc tcggccggcg cttcggcgac gacgccgtcg aacaccgccc aggacgcccg
     2041 gaccagcgcg gcgtgccgct cggcctccgc gctggtcaac ggttgggcat cgctcgcagc
     2101 ccactctccg ggcgcgccga agacggtcgt ggccgtccca gggagtcgct cgacaacggc
     2161 gtaggagaca tccccagccg tgaccgggaa tgcgaccccc gcctgccggg caacgatcga
     2221 gtagcgcggg atataggcct ggagcgcctc gagcgcgagc tcctcgttct tgccggagcg
     2281 gcaccagccc ggccagtcga gcgcgcaggc caagacacgc ttccgcccca cctcgaggta
     2341 gacag
//
LOCUS       DASPWO010000074         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_268155, whole genome shotgun sequence.
ACCESSION   DASPWO010000074 DASPWO010000000
VERSION     DASPWO010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268155"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VEO00_01690"
     CDS             complement(<1..249)
                     /locus_tag="VEO00_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56748.1"
                     /translation="MRRRQTPILVVALVLAGCAATPGKELLPRGSLGPVTEEVSREAV
                     LGLCEARAEVERDPAAAGDAFFDRAHDRLHVIAAAVEEV"
     gene            complement(239..1243)
                     /locus_tag="VEO00_01695"
     CDS             complement(239..1243)
                     /locus_tag="VEO00_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYU56749.1"
                     /translation="MTRRAHPELAAPDSDLEDALRQGLDEVEAQLEKAVVSPTPIVAR
                     AASHLISAGGKRFRPMLVLLSGRFGDPADSRLSRSAVAVELTHLATLYHDDVIDEADH
                     RRGIESVNHHWDNAVAVLAGDYLFARASELATELGTDVSRLLARTVAQLCDGQIREVI
                     AAGRLDQTVGDYMEVIRRKTAALIATSCRLGAMMSAADPEVADRLEAFGEALGNAFQL
                     SDDIMDVIASEETLGKEPGVDLKEGVYTLPVLYALQGARGADLRAVLEPGPPSGERLR
                     AALEIVRDEGTLARARDAVTGEAARAIQAAEGLPEGAPREALEHLATFLAARCGADAP
                     "
     gene            complement(1293..>2513)
                     /gene="nuoN"
                     /locus_tag="VEO00_01700"
     CDS             complement(1293..>2513)
                     /gene="nuoN"
                     /locus_tag="VEO00_01700"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HYU56750.1"
                     /translation="VFLRLVILAVAAGAVLLAAQYHRAAPEEHRAEFYPLLLLAASGM
                     TLLASAGNLLVLFLALETLSLALYVLTGFSPRRFASQEGSVKYFLLGAFSSAFLLYGI
                     AMAYGATGTTSLGGIARALSGQTEPSAFALVAMGLLTVGLGFKVAAVPFHMWTPDVYQ
                     GAPTPVTAFMSAGTKVAGFAALLRVFLVALSPLRWDWIPFMWALAALTMVVGSVLAIA
                     QTDVKRMLAYSSIAHAGFILVGVTAASGEGVSAAMFYLAAYAAMILGAFGVVMLVSGR
                     GEERTGLTAYAGLASRSPLLAALLSLFLLSLAGIPPTAGFIAKVAVFGAVVRAGFWPL
                     ALVAVLASVVAAFFYIRVIVLMYMQEAEETGALAVGPVAGTAVVAPAVLTLLFGVFPA
                     ILFGFLDSAAVLRW"
BASE COUNT          401 a          917 c          924 g          271 t
ORIGIN      
        1 gacctcctcg accgcggcgg cgatgacgtg caggcggtcg tgggcccggt cgaagaacgc
       61 gtcgccggcc gcggcggggt cccgctcgac ctcggcgcgg gcctcgcaca ggccgagcac
      121 ggcctcgcgc gagacctcct cggtgacggg gccgagcgac cctcggggca ggagctcctt
      181 cccgggtgtg gcggcgcacc cggcgaggac cagcgcgacg acgaggatcg gggtctgcct
      241 acggcgcatc ggcgccgcag cgggcggcga ggaacgtcgc caggtgctca agggcctcgc
      301 gcggcgctcc ctcggggagg ccctcggcgg cctggatcgc ccgcgcggct tccccggtga
      361 cggcatcacg ggcccgggcc agcgtgccct cgtcccggac gatctcgagc gcggcgcgca
      421 ggcgctcgcc ggagggcggt ccgggctcca gcacggctcg caggtcggcg ccgcgcgccc
      481 cctgcagcgc gtagagcacc gggagcgtgt agacgccctc cttcaggtcg acccccggct
      541 ccttccccag cgtctcctcg ctggcgatga cgtccatgat gtcgtcggag agctggaagg
      601 cgttcccgag cgcctccccg aacgcctcca gccggtcggc cacctccgga tcggcggccg
      661 acatcatcgc gcccagccgg caggaggtcg cgatcagcgc cgcggtcttc cgccggatga
      721 cctccatgta gtccccgacc gtctggtcca gccggccggc cgcgatgacc tcgcggatct
      781 gcccgtcgca gagttgggcg acggtccggg ccaggagccg cgagacgtcg gtcccgagct
      841 cggtggccag ctccgaggcc cgcgcgaaga ggtagtcgcc ggcgagcacc gccaccgcgt
      901 tgtcccagtg gtggttcacc gactcgatgc cccggcggtg atcggcctcg tcgatcacgt
      961 cgtcgtggta cagggtggcc aggtgggtca gctcgacggc cacggccgag cgcgagaggc
     1021 gcgagtcagc cgggtcgccg aaccggccgg agaggagcac gagcatcggc cggaaccgct
     1081 tgccgccggc ggagatcagg tgcgaggccg cccgggcgac gatcggggtc ggggagacga
     1141 ccgccttctc gagctgcgcc tcgacctcgt ccaggccctg gcgcagggcg tcctcgaggt
     1201 cgctgtcggg cgccgccagc tccgggtgcg cgcgccgggt cacgaacgca tgctaccggc
     1261 cgcaggagga acgccgacca ccggaggaac gcctaccacc tgaggaccgc cgcgctgtcg
     1321 aggaagccga acaggatcgc cgggaagacc ccgaagagga gcgtcaggac ggcgggggcc
     1381 acgaccgccg tcccggccac cggcccgacg gcgagggccc cggtctcttc cgcctcctgc
     1441 atgtacatca gcacgatcac ccggatgtag aagaaggcgg ccaccacgct cgccagcacg
     1501 gcgacgagcg ccaggggcca gaatccggcc cggacgaccg ccccgaacac cgccaccttg
     1561 gcgatgaaac cggccgtcgg cgggatgccg gcgagggaga gcaggaagag cgacaggagc
     1621 gccgcgagca gcgggctgcg ggacgccagg ccggcgtagg cggtcagccc ggtccgttcc
     1681 tcgccccgac cggagacgag catgaccacg ccgaacgcgc cgaggatcat cgcagcgtag
     1741 gcggccaggt agaacatcgc cgccgacacg ccttcgccgc tggccgcggt gacccccacc
     1801 aggatgaacc cggcgtgggc gatcgaggag taggcgagca tccgcttcac gtcggtctgc
     1861 gcgatggcca acacgctccc cacgaccatg gtcagcgccg cgagcgccca catgaacggg
     1921 atccagtccc agcgcagggg cgagagcgcc accaggaaca cgcgcaggag ggccgcgaac
     1981 ccggccacct tcgtcccggc cgacatgaag gcggtgaccg gggtcggcgc cccctggtac
     2041 acgtcgggcg tccacatgtg gaacggcacc gcggcgacct tgaaccccag cccgacggtc
     2101 aggaggccca tggccaccag cgcgaatgcc gacggctcgg tctggccgga cagcgcccgg
     2161 gcgatccctc cgaggctcgt cgtcccggtg gcgccgtagg ccatcgcgat cccgtacagc
     2221 aggaatgccg aggagaaggc accgagcagg aagtacttca ccgagccctc ctgcgaggcg
     2281 aaccgccgcg gcgagaagcc ggtcaggacg tagagcgcca gggagagggt ctccagggcc
     2341 aggaacagca ccagcaggtt ccccgccgac gccaggagcg tcatcccgga ggcggcgagc
     2401 aggagcagcg ggtagaactc ggcgcggtgc tcctccgggg cggcgcggtg atactgggcc
     2461 gccagcagga cggcccccgc ggccaccgcc aggatcacca ggcggaggaa gac
//
LOCUS       DASPWO010000075         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_944398, whole genome shotgun sequence.
ACCESSION   DASPWO010000075 DASPWO010000000
VERSION     DASPWO010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944398"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1839)
                     /locus_tag="VEO00_01705"
     CDS             complement(<1..1839)
                     /locus_tag="VEO00_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HYU56751.1"
                     /translation="MLAPAPPRPDDKTFAAVGTADQWLRCAHVDTALDADVVSLARTP
                     GAAAAGTPSAGTIPVWLAFDPGGCLYRSDPSTGQVQRIVWRPTDPLSGHRDERPPVDL
                     FAAEPATPLGDFSPAGSPAAPSFAPTALAVDADEHLFLLDGTTGSVLVLDLGDGRLLW
                     TVRLPVAVVDLASEGRTILLATADPDRPLYRMEARSEPREVAVPPEAFAGVEAGWVPA
                     RVAAGPGGEVWLLARTPDGRGWAVPVADARAGAAIGPFPFASDVELDGEDRLVVAGRP
                     GEDLLRFEFEDGVVAEGKPLQAPRWTAGGLVRTPDGRIGFWTDHGFRVAVEARPRYEE
                     HGSVDLFRLDAGVYRTQWGRIFVEACIPGGTSVRAAFVTSDDEPGETENDGPSIARTV
                     PSNLTASIRLPRAWPPLVAASRVPELARLRPLHRRETGMDVAWARRDPDDRFEVYEAP
                     VAAPPGRYLWVRLDLRGTRGASPRVRAARVEHPGHDWRSRLPRVYSDDPAAASFLDRY
                     LAMPAGVLADVEERSTMRDVLLDPFGAPADVLPWLASLVGMTLDDRWPEPARRRMLSE
                     AVCLFRRRGTIAALRRMIEIYLGCQVIVIERFRLRGLGGGLLGDEGA"
     gene            complement(1839..4124)
                     /locus_tag="VEO00_01710"
     CDS             complement(1839..4124)
                     /locus_tag="VEO00_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HYU56752.1"
                     /translation="MAVPVPRLDDRSFDDIVAELRNRIPVYTPEWTDLGASDPGITLL
                     ELFAFLGENLLYRFNQIPDATKMWLVRLLQVPPRPARSASGLVAFTTRAPTSASAATV
                     EQGASVRAGKVPFRVQWDVAVMPLDVRVAVKTATDPPTDPELVQSVERALDARDGLAE
                     GEEPQFYETDVLAPDPTAPDFQPLDVSLAVDRSLWVAVFSTGADPTELLKPGGPLDTN
                     ALTIGVGVDAEFPGIDDVDPCDGLDAPPERARVATASLERQLMECGLAPQLGKDGTTS
                     ENDRAEPPLVFWKVSTVVPGDDGEPEYRDVAVVGDTTDGMTRDGVISLRLPDRLEGVG
                     VFEFSEPDRAGTGSLPPPIEDDRPVLFWLRAFPREGVPDVGRLRWVGVNAASVDQVAQ
                     AVPEFLGVGTGMPNQELQLAQTSVVADSVVLEVENDGRWDRWQRVDGFAASLRTDLHF
                     VLDAVAGRVTGGNTVRGRAFPMDRRIRVRGATGGGYLYGGGRRGNVAAKAISTVDAGV
                     DVTVANPLPTTGGEEAESITAALERIPGELARHDRAVTSSDFRELAAITAVGRAECLP
                     RFHPPTRQWEAAGVVSVVVWPTEDPKHPNAPVPDAGLLRAVCRQLDARRLVTTELYVI
                     PPVYRKVAVSVGIAVKPGYSAAAVRRWVELVVRQYLAPLPPYGPEGQGWPLGHRVYGP
                     ELEAAVLQVEGVDFLEGLEVAGLAADGTWRQGTVELDGWEVVELAELSVVAGPPTPAG
                     TDVPPPPAPTLVPIPVPKEEC"
     gene            complement(4127..>4519)
                     /locus_tag="VEO00_01715"
     CDS             complement(4127..>4519)
                     /locus_tag="VEO00_01715"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HYU56753.1"
                     /translation="LTGWTFPPMPPPERGPMSFLDGPALVRQSIRLILETEPGERVMR
                     PTFGCGLRRYLMEPNTPSTRAGVGRDVEAALRAWEPRIDLRQVDVATTQDPSEIVVTI
                     SYEHVRDRTPAVLQVPVVLGGAVTGAGS"
BASE COUNT          598 a         1724 c         1669 g          528 t
ORIGIN      
        1 cgcgccctcg tcgcccagca acccgccccc caatccgcgc aggcggaagc gttcgatgac
       61 gatgacctgg cagcccaggt agatctcgat catccggcgg agcgcggcga tggtcccccg
      121 ccggcggaac aggcacacgg cctccgagag catcctccgg cgtgccggct cgggccaccg
      181 gtcgtcgagg gtcatcccga cgagggaggc cagccacggc agcacatcgg ccggggcgcc
      241 gaaggggtcg agcagcacgt cgcgcatcgt cgagcgctcc tccacgtcgg cgagcacgcc
      301 ggccggcatc gccaggtacc ggtcgaggaa cgacgcggcg gcagggtcgt ccgagtacac
      361 gcgcgggagg cgggaccgcc agtcgtgccc cgggtgctcg acccgcgcgg cccggaccct
      421 cggggaggcc cccctggtcc cccgcagatc gagccggacc cacaggtagc ggccgggcgg
      481 cgcggccacc ggcgcctcgt acacctcgaa ccggtcgtcc gggtcgcgcc gcgcccaggc
      541 gacgtccatg ccggtctccc ggcggtggag cggccggagc ctcgccagct ccggaacgcg
      601 cgaggcggcg acgagcggcg gccaggccct cggcagccgg atgctcgccg tcaggttcga
      661 ggggaccgtc cgggcgatgc tgggcccgtc gttctcggtc tccccgggct cgtcgtcgga
      721 cgtgacgaac gcggcgcgca ccgacgtacc gcccgggatg caggcctcga cgaagatgcg
      781 cccccactgc gtccggtaga cgccggcgtc gaggcggaac aggtcgacgc tcccgtgttc
      841 ctcgtagcgg ggacgcgcct cgacggcgac ccggaagccg tggtccgtcc agaacccgat
      901 gcgcccgtcg ggcgtccgga cgagcccgcc agccgtccac cggggcgcct gcaggggctt
      961 gccctcggcg accacgccgt cctcgaactc gaaccgcagc aggtcctccc ccggccgacc
     1021 ggccacgacc agccggtcct cgccgtcgag ctccacgtcc gatgcgaacg ggaacggccc
     1081 aatggcggcg cccgcccggg cgtcggcgac cgggaccgcc cagccgcggc cgtcgggcgt
     1141 gcgcgccagg agccacacct cgcccccggg ccccgccgcc acccgcgcgg gcacccaccc
     1201 ggcctccacg ccggcgaacg cctcgggcgg gacggccacc tcgcgcggct cgctccgggc
     1261 ctccattcgg tacagcggcc gatcggggtc ggcggtggcc aacaggatcg tccggccctc
     1321 cgaggccagg tcgaccacgg cgacggggag ccggaccgtc cacagcagcc tgccgtcacc
     1381 gaggtcgagc accagcaccg agcccgtggt cccgtcgagc aggaacaggt gctcgtccgc
     1441 gtcgacggcc agcgcggtcg gcgcgaacga cggcgcggcc ggcgaccccg ccggcgagaa
     1501 gtcgccgagc ggggtcgcgg gttccgcggc gaacaggtcg accggggggc gttcgtcgcg
     1561 gtgcccggac agcgggtcgg tcgggcgcca tacgatccgc tggacctgtc cggtcgacgg
     1621 gtcgctccgg tacaggcacc caccgggatc gaacgccaac cagacgggga tcgtccccgc
     1681 cgacggcgtt cccgccgccg cggcgcccgg ggtccgggcg agggagacca cgtcggcgtc
     1741 gagcgcggtg tcgacgtggg cgcagcgcag ccactggtcg gccgtcccga ccgcggcgaa
     1801 cgtcttgtca tccggccggg ggggcgcggg cgcgagcatc agcactcctc cttcgggacc
     1861 gggatcggga cgagcgtcgg cgccggcggc gggggcacgt cggtcccggc cggcgtcggc
     1921 ggcccggcca cgacgctcag ctccgcgagc tcgacgacct cccacccgtc gagctcgacc
     1981 gtcccctgcc gccaggtccc gtcggcggcg agcccggcca cctcgagccc ctccaggaag
     2041 tcgacgccct cgacctgcag gacggccgcc tcgagctcag gtccgtacac gcggtggccg
     2101 agcggccacc cctggccctc ggggccgtac ggcgggagcg gggcgaggta ctggcggacc
     2161 acgagttcga cccaccggcg gaccgcggcc gcgctgtacc cgggcttgac cgcgatcccc
     2221 accgacacgg ccaccttccg gtacacgggc gggatcacgt agagctcggt ggtcacgagg
     2281 cgccgcgcgt cgagctgccg gcacaccgcg cggagcaggc cggcgtcggg gaccggggcg
     2341 ttcgggtgct tcgggtcctc ggtgggccac acgacgacgg acacgacgcc ggccgcctcc
     2401 cactggcgcg tcggcgggtg gaaccggggg aggcactcgg cccgcccgac ggcggtgatc
     2461 gcggccagct cccggaagtc gctgctcgtc acggcccggt cgtggcgggc cagctccccg
     2521 gggatgcgct cgagcgccgc ggtgatgctc tcggcctcct ccccacccgt cgtcgggagc
     2581 gggttcgcga ccgtgacgtc cacacccgcg tcgacggtcg agatggcctt cgcggcgacg
     2641 ttcccccgcc ggcctccgcc gtacaggtag ccgccgccgg tcgccccgcg aacgcggatc
     2701 cggcggtcca tcggaaacgc ccggccccgg accgtgttgc cgccggtcac ccggccggcc
     2761 acggcgtcga gcacgaagtg gaggtcggtg cgcaggctcg cggcgaagcc gtcgacgcgc
     2821 tgccaccggt cccaccgccc gtcgttctcg acctccagga cgacgctgtc ggccacgacg
     2881 gacgtctgcg cgagctggag ctcctggttc ggcattccgg tcccgactcc gaggaactcc
     2941 ggcaccgcct gcgctacctg gtcgacggag gcggcgttca cgcccaccca ccgcagtcgc
     3001 ccgacgtcgg gcacgccctc gcgggggaac gcccggagcc agaacagcac cgggcggtcg
     3061 tcctcgatcg gcggcggcaa cgacccggtg cccgcgcggt ccggttcgga gaactcgaac
     3121 acgccgaccc cctccagccg gtcggggagg cggagcgaga tgacgccgtc gcgcgtcatc
     3181 ccgtcggtgg tgtcgcccac gacggcgacg tcccggtact cgggctcgcc gtcgtcgccc
     3241 ggcaccaccg tggacacctt ccagaagacc agcgggggct cggcccggtc gttctccgac
     3301 gtggtgccgt ccttcccgag ctggggggcg agcccgcact ccatcagctg gcgctcgagc
     3361 gacgcggtcg cgacgcgggc ccgctcgggg ggcgcgtcga gcccgtcgca cgggtcgacg
     3421 tcgtcgatgc ccgggaactc tgcgtcgacg ccgacgccga tcgtgagggc gttcgtgtcg
     3481 agcgggccgc cgggcttcag cagctccgtg ggatccgcgc cggtcgagaa cacggcgacc
     3541 cacagcgaac gatcgacggc gagcgacacg tcgagcggct ggaagtccgg cgccgtgggg
     3601 tcgggcgcga gcacgtcggt ctcgtagaac tggggctcct ccccctccgc gagcccgtcg
     3661 cgggcgtcca gcgcccgttc gacgctctgc acgagctctg ggtccgtcgg cgggtcggtc
     3721 gcggtcttca cggccacccg aacgtcgagc ggcatcacgg ccacgtccca ctgcacgcgg
     3781 aacggcacct tccccgcccg gacggaggct ccctgttcca ccgtcgcggc cgaggccgac
     3841 gtcggggccc tggtcgtgaa cgccaccagg ccggacgcgc tcctcgcggg ccggggcggt
     3901 acctggagca gccggaccag ccacatcttg gtggcgtccg ggatctggtt gaagcggtac
     3961 aggaggttct cgccgaggaa cgcgaacagc tcgagcaggg tgatgccggg gtcgctcgcc
     4021 ccgaggtcgg tccactcggg cgtgtagacc gggatccggt tgcggagctc ggcgacgatg
     4081 tcgtcgaacg atcggtcgtc gagccggggg accgggaccg ccacggtcag ctccccgctc
     4141 ccgtgacggc gccaccgagg acgaccggaa cctgcagcac cgccggggtc cggtcgcgca
     4201 cgtgctcgta cgagatggtc acgacgatct cggacgggtc ctgggtcgtc gccacgtcga
     4261 cctgacggag gtcgatgcgc ggctcccacg cccggagggc ggcctcgacg tcgcggccga
     4321 cgccggcgcg ggtcgagggg gtgttcggct ccatcaggta ccggcgaagg ccgcagccga
     4381 acgtcggccg catgacccgc tcgcccggct ccgtctcgag gatcagccgg atcgactggc
     4441 ggacgagcgc ggggccgtcc aggaacgaca tcggcccacg ctcgggcggt ggcatcggcg
     4501 gaaaggtcca accggtgag
//
LOCUS       DASPWO010000076         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_691451, whole genome shotgun sequence.
ACCESSION   DASPWO010000076 DASPWO010000000
VERSION     DASPWO010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691451"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1484
                     /gene="gabT"
                     /locus_tag="VEO00_01720"
     CDS             144..1484
                     /gene="gabT"
                     /locus_tag="VEO00_01720"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HYU56754.1"
                     /translation="MDIEQARRIVTEIPGPRSRELAARRAAAIPRAVFNVAPVFVERA
                     QGAVVEDVDGNRLIDFAAGLAVLNAGQTHPAVVAAVREQAARYLHTCFHVAMNEPYLV
                     LAERLNALTPGAFDKRTLLVNSGAEAVENAVKIARYATGRPGVVVFDHGFHGRTLLAM
                     TMTAKVMPYKKGFGPFAPEVHRLPFAYPYRCPAGAAPEDCGEACAAYAIDEMTHHVGP
                     DQIAAVVVEPVLGEGGFVVPGTGFLEAIRRFTADHGIVFVADEVQTGFGRTGRWFASE
                     HFGIEPDLVAMAKALGGGLPLGAVTGRADLMEAPHVGGLGGTFGGNPLACAAALAVID
                     TLDGERLIERAARIGEVIAARFREFRDRFEGVGDVRGLGAMQAIEFVGDRTTKAPSKA
                     VAARVLEEALREGLLLMKAGTYDNVIRLLPPLSIDDGLLDEGLSIIEKALSAAS"
     gene            complement(1453..1668)
                     /locus_tag="VEO00_01725"
     CDS             complement(1453..1668)
                     /locus_tag="VEO00_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56755.1"
                     /translation="MRTEVTGTRAELKADIADSRAEFRAGFLEVHRELAEMRSDLTRV
                     AVGRRGRAAGGRPVGLLVRTRPTGPSR"
     gene            1800..2114
                     /locus_tag="VEO00_01730"
     CDS             1800..2114
                     /locus_tag="VEO00_01730"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HYU56756.1"
                     /translation="MAALSEDQVREGIADLPGWRLGKGEIVRNYLFPTFRDAVDFIVR
                     IAEHAEAANHHPDLENSYNRVRIALHTWSENAITEKDIALAHAIESEAGKARPPSRRS
                     EA"
     gene            complement(2103..2606)
                     /locus_tag="VEO00_01735"
     CDS             complement(2103..2606)
                     /locus_tag="VEO00_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HYU56757.1"
                     /translation="MGWRLVPEIPQSMCFGCSPRNHAGLGRHTVYRLQDDGAVRADYV
                     PPEHHGGGGRVVHGGVLTALLDEAAGFLVATLTDQGFFTVGMQVAFRGPATIGEPVTA
                     VARLAERRRRSLAVHVEARAGTMLAARGEVDYFTVPPERYVEMTGFPDVPSAFLRMVP
                     DDEGSGL"
     gene            complement(2613..2870)
                     /locus_tag="VEO00_01740"
     CDS             complement(2613..2870)
                     /locus_tag="VEO00_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56758.1"
                     /translation="MTIELTERAVEILGRSYEAARRYNPDVHLRLARRGPSVEALLAE
                     APEEGDERVEGPGFVLLVEAGLDGRLEVHEPHDRLVLVPRA"
BASE COUNT          427 a         1090 c         1077 g          393 t
ORIGIN      
        1 gagcggggca tcgcggtccg cgccacccgg tcgcgcgccg gacgccgtgg cgagatggag
       61 ggtgtggagg ggtagctcgc cccaggaccg gatcgacccg accatgccgg cgagcggacc
      121 caaggaagga cgagggaggg cgcatggaca tcgagcaggc acgccggatc gtcaccgaga
      181 tccccggccc gcggtcgcgc gagctggccg cgcggcgagc ggcggcgatc ccgcgtgcgg
      241 tgttcaacgt ggcgccggtg ttcgtcgagc gggcgcaggg cgccgtcgtc gaggacgtgg
      301 acggcaaccg cctgatcgac ttcgccgccg gtctcgccgt gctgaacgcc ggccagaccc
      361 acccggcggt ggtcgctgcg gtccgcgagc aggcggcgcg ctacctgcac acctgcttcc
      421 acgtcgcgat gaacgagccc tacctggtgc tggccgagcg cctgaacgcg ctgacgccgg
      481 gcgcgttcga caagcgcacg ctcctcgtca actcgggcgc cgaggccgtc gagaacgccg
      541 tgaagatcgc ccgctacgcc acaggccgcc cgggggtggt ggtcttcgac cacggcttcc
      601 acggccggac gctcctggcg atgacgatga ccgcgaaggt gatgccgtac aagaagggct
      661 tcggcccgtt cgcccccgag gtccaccgcc tgccgttcgc ctacccgtac cgctgcccgg
      721 ccggcgccgc acccgaggac tgcggcgagg cctgcgccgc gtacgcgatc gacgagatga
      781 cccatcacgt cggcccggat cagatcgccg ccgtggtcgt cgagccggtg ctcggcgagg
      841 gcggattcgt cgtccccggc accggcttcc tcgaggcgat ccggcggttc accgccgacc
      901 acggcatcgt gttcgtcgcc gacgaggtgc agaccggctt cggccggacc ggccgctggt
      961 tcgcatcgga gcacttcggg atcgagcccg acctggtggc gatggcgaag gccctcgggg
     1021 gcggactgcc gctcggggcc gtcaccggcc gggccgatct gatggaggcg ccgcacgtcg
     1081 ggggcctcgg cgggaccttc ggcggtaacc cgctggcgtg cgccgccgcg ctcgccgtca
     1141 tcgacacgct cgacggcgag agactgatcg agcgcgcggc ccggatcggg gaggtcatcg
     1201 ccgcccgctt ccgggagttc cgcgaccgct tcgagggggt cggcgacgta cgcgggctcg
     1261 gcgcgatgca ggcgatcgag ttcgttggcg accggacgac caaggcgccg tcgaaggcgg
     1321 tcgcggcgcg ggtcctggag gaggccctgc gcgaggggct gctcctcatg aaggccggga
     1381 cctacgacaa cgtgatccgc ctgcttccgc ctctctcgat cgacgacggg ctcctcgacg
     1441 agggcctgtc gatcatcgag aaggccctgt cggccgcgtc ctgaccagga gccctactgg
     1501 ccggccgccc gccgcccggc cccgacggcc caccgcgacc cgggtcaggt ccgagcgcat
     1561 ctcggccagc tcacggtgca cctccaggaa gccggcgcgg aactccgcgc gtgaatcggc
     1621 gatgtccgcc ttcagctccg cgcgcgtgcc ggtgacctcc gtccgcacgt ctcggatccc
     1681 ggaccgcacg tctagacctc tgaccggacg tctcggatct cggaccggat gatcgtaagc
     1741 agggagcacc gacatgacgg cgcttgacgg ttcgctcgag aggcgacacc gtgcgaggta
     1801 tggccgcgct ctccgaggac caggtgcggg aggggatcgc cgacctgccg ggatggcggc
     1861 tgggcaaggg cgagatcgtc cggaactacc tgttcccgac gttccgcgac gccgtggact
     1921 tcatcgtccg gatcgccgag cacgcggagg ccgcgaacca ccaccccgac ctggagaact
     1981 cctacaaccg cgtccggatc gccctgcaca cctggagcga gaacgcgatc accgagaagg
     2041 acatcgcgct ggcgcacgcg atcgagtccg aggctgggaa ggcgcggccc ccgagccggc
     2101 gatcagaggc ctgagccctc gtcgtcgggg accatccgca ggaacgccga gggaacgtcc
     2161 gggaagccgg tcatctccac gtagcgctcg ggcgggacgg tgaagtagtc cacctccccg
     2221 cgggcggcga gcatcgtccc cgcgcgcgcc tccacgtgga ccgccagcga ccggcgccga
     2281 cgctcggcca ggcgggcgac cgccgtgacc ggttcaccga tcgtggccgg acccctgaac
     2341 gcgacctgca ttcccacagt gaagaatccc tggtcggtca gcgtcgccac caggaacccg
     2401 gccgcttcgt cgaggagcgc ggtgagcacg ccgccgtgga cgaccctgcc tcccccgccg
     2461 tggtgctcgg ggggcacgta gtcggctcgg accgcgccgt cgtcctgcag ccggtacacc
     2521 gtgtgccggc cgagccccgc gtggttccgc ggcgagcagc cgaagcacat cgactgcggg
     2581 atctccggga ccagccgcca ccccacggcg cgtcaggccc gcgggacgag gaccaggcgg
     2641 tcgtgcggct cgtgcacctc gagccgtccg tcgagcccgg cctcgacgag cagcacgaac
     2701 ccggggccct ccaccctctc gtctccctcc tccggcgcct cggcgagcag cgcttcgacc
     2761 gacggccccc tgcgggccag gcgcaggtgg acgtccgggt tgtagcgccg cgcggcctcg
     2821 tacgaccgcc ccaggatctc caccgcccgc tcggtgagct cgatggtcac ggcagcatct
     2881 tgcactacgc tacgcgagcg atgggagcga gagcggtgcc gttatggcgt ttcgcgggcg
     2941 agcccggcgg ttgtgggacg cgttgcgggc ggagcggcgc acgctcc
//
LOCUS       DASPWO010000077         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1198689, whole genome shotgun sequence.
ACCESSION   DASPWO010000077 DASPWO010000000
VERSION     DASPWO010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198689"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /gene="nusG"
                     /locus_tag="VEO00_01745"
     CDS             <1..738
                     /gene="nusG"
                     /locus_tag="VEO00_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HYU56759.1"
                     /translation="DAPAAPGAEAAAAESFEAPAAEPGPGPLEEPAIEPAAQPEAPAA
                     EEPAEERIEVSEDEETVRWSPFEGPGDWYVVHTYAGYENKVKANLESRIASMQMEEKI
                     FDVVIPMEDVMEIKGGKKQVVQKKVFPGYLLVKMLYDNDSWYVVRNTPGVTGFVVSGT
                     GAKPTPLSRREVEKILAVKEEEKAKPAFRLEFEEGDMVRIISGPFADFNGVIGEINVD
                     QSKVKVMVNIFDRETPVELGFDQVARV"
     gene            800..1234
                     /gene="rplK"
                     /locus_tag="VEO00_01750"
     CDS             800..1234
                     /gene="rplK"
                     /locus_tag="VEO00_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HYU56760.1"
                     /translation="MAVRKKKILAVVKMQLRAGQASPAPPVGTALGQHGVNIMDFVKQ
                     YNDATQAQMGQIVPVEMTIYEDRSFTFFTKQPPAAELLRQAAGIEKGSGEPNRAKVGR
                     VTRDQVRRIAETKIKDLNANDVENAMRIIEGTARSMGIEVAS"
     gene            1231..1944
                     /gene="rplA"
                     /locus_tag="VEO00_01755"
     CDS             1231..1944
                     /gene="rplA"
                     /locus_tag="VEO00_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HYU56761.1"
                     /translation="MKRGKRYDAAAALVDRDRLSEPREAMSLLKRFPESKFDETVEVA
                     LRLGLDPRKADQQIRGTVDLPHGTGRSVRVAAFASGDKGREAREAGADLVGGEDLVEQ
                     VLGGTIDFDAAVATPDMMSAVGKAGRVLGPRGLMPNPKAGTVTFDIGKAVRDIKAGKL
                     EYRLDRQGNVHLVIGKRSFEAEALMENYQAIIEEILRAKPSAAKGRYLKSVTLSTTMG
                     PGIRIDPARARGLVEAATA"
     gene            2111..2776
                     /gene="rplJ"
                     /locus_tag="VEO00_01760"
     CDS             2111..2776
                     /gene="rplJ"
                     /locus_tag="VEO00_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HYU56762.1"
                     /translation="MPAATKVERVRELKERLQGSQAALVSDYRGITVKDATELRRALR
                     DAEATFTIVKNTLTRLAAAEAGFEELVAILEGPSAITFVRGDVVLAARRLADAAKRIP
                     ALELKGGVMEGRVLSAEQARGLATLESREVMLARLAGLAKAQMSQAAYLFQATLAHFL
                     RLLEAYKEKLPPGEAPTAEEAPAGEAPAGEVPAEEAAGEGDVQISAQAEALAEEASED
                     GKE"
     gene            2796..3182
                     /gene="rplL"
                     /locus_tag="VEO00_01765"
     CDS             2796..3182
                     /gene="rplL"
                     /locus_tag="VEO00_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HYU56763.1"
                     /translation="MTADDLLEAFKEMTLIELSEFVKKFEETFEVQAAAMAPMAMAGA
                     VGGAVAAPAEEAEEQTEFDVVLTGAGAEKIKVIKEVRTLTSLGLKEAKDLVDNAPGTV
                     LERVTKEQAEEARAKLEGVGAGVEVK"
BASE COUNT          587 a         1073 c         1334 g          422 t
ORIGIN      
        1 gacgcgccgg ccgccccggg cgccgaggcg gctgccgccg agtccttcga ggcgcccgcc
       61 gccgagccgg gccccgggcc gctcgaggag ccggccatcg agccggccgc ccagcccgag
      121 gcgccggccg cggaagagcc cgccgaggag cgaatcgagg tgtccgagga cgaggagacc
      181 gtgcgctggt cgccgttcga ggggccgggc gactggtacg tggtgcacac ctacgccggc
      241 tacgagaaca aggtgaaagc caacctcgag tcgcgcatcg cctcgatgca gatggaggag
      301 aagatcttcg acgtcgtcat cccgatggag gacgtcatgg agatcaaggg cggcaagaag
      361 caggtcgtcc agaagaaggt ctttcccggc tacctgctgg tgaagatgct ctacgacaac
      421 gactcctggt acgtcgtgcg caacacgccc ggcgtgaccg ggttcgtcgt gtcgggcacg
      481 ggcgccaagc cgacgccgct ctcccggcgc gaggtcgaga agatcctcgc ggtcaaggag
      541 gaggagaagg ccaagccggc gttccgcctg gagttcgagg agggcgacat ggtgcggatc
      601 atcagcgggc cgttcgccga cttcaacggc gtgatcggcg agatcaacgt agaccagtcg
      661 aaggtcaagg tgatggtcaa catcttcgac cgggagaccc cggtcgagct cggattcgac
      721 caggtcgccc gcgtctagac tcgggcggca caccggggcg ggagggcgcg aggccgcagg
      781 accgcaggag gtacgcgaga tggcagtacg gaagaagaag atcctcgccg tcgtgaagat
      841 gcagctgcgc gccggacagg cgtccccggc cccgcccgtc ggcacagcgc tcggccagca
      901 cggcgtgaac atcatggact tcgtcaagca gtacaacgac gccacgcagg cgcagatggg
      961 ccagatcgtg ccggtcgaga tgacgatcta cgaggatcgc tcgttcacgt tcttcaccaa
     1021 gcagccgccc gccgccgagc tgctgcgcca ggccgccggc atcgagaagg gctccggcga
     1081 gccgaaccgg gccaaggtcg ggcgggtgac gcgcgaccag gtccggcgga tcgccgagac
     1141 caagataaag gacctcaacg cgaacgacgt cgagaacgcg atgcgcatca tcgagggcac
     1201 cgcgcggagc atgggcatcg aggtggcttc gtgaaacgcg gcaagcgcta cgacgccgcg
     1261 gcggcgctcg tcgaccgcga caggctctcc gaaccgcgcg aggccatgtc gctcctgaag
     1321 cggttcccgg agtcgaagtt cgacgagacc gtggaggtgg cgctgcgctt ggggctcgat
     1381 ccccgcaagg ccgaccagca gatccgcggg acggtggacc tgccgcacgg gaccggcagg
     1441 tcggtgcgag tggcggcctt cgcgtccgga gacaagggcc gcgaggcgcg cgaggccggg
     1501 gcggaccttg tcgggggcga ggacctggtc gagcaggtgc tcggcgggac catcgacttc
     1561 gacgcggccg tcgccacgcc cgacatgatg tcggcggtcg gcaaggccgg gcgggtgctc
     1621 gggccgcgcg ggttgatgcc caacccgaag gcggggacgg tgacgttcga catcggcaag
     1681 gccgtgcgcg acatcaaggc cggcaagctc gagtaccgcc tggaccggca ggggaacgtg
     1741 cacctggtga tcggcaagcg ctcgttcgag gcggaggccc tgatggagaa ctaccaggcg
     1801 atcatcgagg agatcctgcg ggccaagccg tcggcggcga agggccgcta cctgaagagc
     1861 gtcacgctgt cgacgaccat gggtccgggc atccgcatcg accccgcccg ggcccgcggc
     1921 ctggtcgagg cggccaccgc gtagcgaagg ttgcgttgac gggtggcgga gacggccgct
     1981 accctggggg cgccgcagac agcaggggcg gaagcgtaat ccccggttcc ggggcacctg
     2041 ccgaggcgac ggggcgaaac gcctccgcgt ctgtttgcgg gggcgttttc tcgtgagcga
     2101 aggagacacg gtgccggcag cgacgaaggt ggagcgggtc cgcgagctga aggagcggct
     2161 gcagggctcc caggccgcgc tcgtctcgga ctaccgcggg atcacggtca aggacgccac
     2221 cgagctgcgc cgggctctgc gcgacgccga ggccacgttc acgatcgtca agaacacgct
     2281 gacccgtctg gccgcggccg aggccgggtt cgaggaactg gtggcgatcc tggagggccc
     2341 gtcagcgatc acgttcgtcc ggggcgacgt ggtgctcgcg gcgaggcggc tggccgacgc
     2401 ggcgaagcgc atccccgccc tggagctgaa gggcggggtg atggaggggc gggtgctgtc
     2461 cgccgagcag gcgcgcggcc tggccacgct cgagagccgc gaggtcatgc tggcgcggct
     2521 ggccgggctg gcgaaggcgc agatgtcgca ggccgcctac ctgttccagg ccacgctggc
     2581 gcacttcctg cgcctgctgg aggcctacaa ggagaagctg cccccgggcg aggcgccgac
     2641 cgctgaggag gctcccgcgg gggaggctcc cgcaggggag gtgcccgcgg aggaggcggc
     2701 cggcgagggc gatgtgcaga tctcggccca ggcggaggcc ctggccgaag aggcttcaga
     2761 ggatggaaag gagtagcgcg gtggcgacca agaagatgac ggccgacgac ctgctcgagg
     2821 cgttcaagga gatgacgctc atcgagctgt cggagttcgt gaagaagttc gaggagacct
     2881 tcgaggtgca ggccgcggcg atggcgccga tggccatggc cggggcggtc ggcggggccg
     2941 tggccgcgcc ggccgaggag gccgaggagc agacggagtt cgacgtcgtg ctcaccggcg
     3001 ccggggccga gaagatcaag gtgatcaagg aggtgcgtac gctcacgagc ctcggcctga
     3061 aggaggccaa ggacctggtg gacaacgcgc ccggcaccgt cctcgagagg gtgaccaagg
     3121 agcaggcgga agaggcccgg gccaagctcg agggcgtcgg cgccggcgtc gaggtcaagt
     3181 agcgaggacg tctccacgcc accctcgacg cctgccgggc ggctctggca tggtgcgggg
     3241 tagctcggga tcggggagga cggtctcggc cacctccggc gagcgcaccc caaggagaag
     3301 ggaccagtgg gtcttcgaga cgcgctgccg gacggggtga agcgcgtgct gcggccggtg
     3361 ctgtcctctc cgccggtgca ctacgcgcgg cgcgcggtgc gggtcggggc gctcgc
//
LOCUS       DASPWO010000078         5468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_269147, whole genome shotgun sequence.
ACCESSION   DASPWO010000078 DASPWO010000000
VERSION     DASPWO010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269147"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1099)
                     /gene="moaA"
                     /locus_tag="VEO00_01770"
     CDS             complement(107..1099)
                     /gene="moaA"
                     /locus_tag="VEO00_01770"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HYU56764.1"
                     /translation="MILPLVDTFGRVASDLRVSLTDRCNLRCTYCMPAEGLPWLARDE
                     HLAAAEIERLVRLFVALGVREVKLTGGEPTARRDLVEVVQRLRAIDPPLDLSMTTNGV
                     LLDRLAAPLRAAGLDRVTVSCDSLLAHRFAEMTRRDALARVRAGLAAAEAAGFAPIKL
                     NCVVIRGTNDEEVLDFALLARETGWEVRFIEYMPLDAERAWERSKVVPAAEIRATIEA
                     AHALSPAGPDGPHPAAVFRFADGAPGAVGFIATVTEPFCDTCNRLRLTADGALRACLF
                     ALEESDLRGPLRAGAADADLEALIRAAVAGKWSGHRINHPDFVRPERSMSMIGG"
     gene            1143..2429
                     /locus_tag="VEO00_01775"
     CDS             1143..2429
                     /locus_tag="VEO00_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HYU56765.1"
                     /translation="MAPSSRRATVLTVSDGVHRGVREDRSGAAVTASLVRAGFAVMQE
                     RVPDERAEIEAALRRLAAEAELVVTTGGTGLGPRDVTPEATRAVVDREAPGLAEAMRA
                     AGRASTPAADLSRGIAGTLGGALVVNLPGSERGAVESLEAVLPVLPHALDLLAGRTEH
                     PGDHPPATAGREANETASADPAAEALQRAAAGEPVVLATAVSVKGDPPCRPGQKLLLG
                     PGGPLAGTLGCAEFDTGAAADAPAVISAGEPALRQYSHELGTVRVYLEPVRRRPALVV
                     LGATPVALWLLRWGRDLGFEPALVESRAERVTPEHRAAAARVADRPEALGPLGGAPAV
                     HTDHDAPLVAEHVKAVIDAGAGFVGVMGSARHGGPHLETLRALGVPPDRVAAVETPVG
                     LDIGARTPQEIALSILAGIVARRGGRDGGPLARRTR"
     gene            2426..3088
                     /locus_tag="VEO00_01780"
     CDS             2426..3088
                     /locus_tag="VEO00_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HYU56766.1"
                     /translation="MRVRVRLFGALAERAGLPEVGLDLPPGATAADACHAALRPYPTA
                     AGLAEHVSIAVNLEVVPGPHRLNEGDEVGLLPPVAGGADGPRILTGLRPGGPDPAEAL
                     AAVTAARAGGIVAFIGSVRTEDDWPVEALEYSAYDEMADRVLTEIAAEVAEKWPLEAV
                     AIVHATGRLPVGAVTFVVACAAPHREEAFAACRHAVEEVKRRAPIWKREVGPHGARWV
                     GL"
     gene            3085..4326
                     /gene="glp"
                     /locus_tag="VEO00_01785"
     CDS             3085..4326
                     /gene="glp"
                     /locus_tag="VEO00_01785"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HYU56767.1"
                     /translation="MTADELLPVATALDLALDGIGPLSPAELSLEAAYGCAAAEDVVA
                     DRFLPSFSSSGMDGFAVRANDVALASPESPVELRITGRVAMGKAPDLAVGSGEAVRIA
                     TGAPVPAGSDGVVPIELCEVFADARVLVRQGVPPGRYLRPAGQDVRPGEVLVREGRRL
                     LGPELGLLALAGRDRVRVRPRPRVAILSTGDELVPPGAMAGFGQVHDANGPTLFGAAK
                     EAGAEPRLAGIVPDDADAFRDALGRAATDTDVLLSSGGVSAGELDVVKRTFEGRGEVA
                     FHRVAMQPGMPQAFGRIDGIPFFGLPGNPVSVFVSFEVFVRPAILRMLGRRRIVRPAI
                     RARVAGELRGQPEKTVFARVRAARNESGWTATSTGGPQSNLLATLSQANGLAVIPPGV
                     ERLGHGEVCRVMLFRDPEETT"
     gene            4323..4778
                     /gene="moaC"
                     /locus_tag="VEO00_01790"
     CDS             4323..4778
                     /gene="moaC"
                     /locus_tag="VEO00_01790"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HYU56768.1"
                     /translation="MSEERAARMVDVGGKEPTERRAVAEGALATTPEACARLTEGTPK
                     GNPLEAARVAGLLAVKRTPDLLPYCHPVAVTGASVDVEPDPATGRIRVRATVEARDRT
                     GVEMEALTAVAVAALSLYDTAKPYDRAARITDIRLVSKEGGKSGRYRAG"
     gene            complement(4795..5115)
                     /locus_tag="VEO00_01795"
     CDS             complement(4795..5115)
                     /locus_tag="VEO00_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005512007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HYU56769.1"
                     /translation="MITLTEMAASKVKELLDQEGRTDIALRVAVQPGGCSGLRYAMYL
                     DDQLADGDLAEEQHGVRIVVDRTSAPYLSQAKVDFVDSLEASGFTIDNPMAQGSCACG
                     HSFH"
     gene            complement(5180..>5468)
                     /locus_tag="VEO00_01800"
     CDS             complement(5180..>5468)
                     /locus_tag="VEO00_01800"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HYU56770.1"
                     /translation="TSGANRADPTRLATLLGAEDVRRAGADEARAATGFAIGGTPPFG
                     YPRPLPVLMDEDLLRHEAVWAASGTPDGVFRTDPQTLLRVTAARVAAFREG"
BASE COUNT          654 a         2038 c         2103 g          673 t
ORIGIN      
        1 ggttcggagg ccgatgccct gcgcgcgcgg gccgacgagc tgctggccgc cacacgcgac
       61 gcgttcgccg ctcgcacgtc tcccgggtaa cccgtggctc gcggcctcag ccgccgatca
      121 tcgacatcga gcgctcgggg cggacgaagt cggggtggtt gatgcggtgg ccggaccact
      181 tgccggcgac ggcggccctg atcagggcct cgaggtcggc gtcggcggcg ccggcccgga
      241 gcggcccgcg caggtcgctc tcctccaggg cgaacaggca cgcgcggagg gccccgtcgg
      301 ccgtcaggcg cagccggttg cacgtgtcgc agaacggctc ggtcacggtg gcgatgaagc
      361 cgaccgcgcc cggcgcgccg tcggcgaacc ggaacaccgc cgcggggtgg gggccgtccg
      421 gcccggccgg ggagagcgcg tgcgccgcct cgatggtcgc ccggatctcg gccgccggca
      481 cgaccttcga gcgctcccag gcgcgttccg catcgagcgg catgtactcg atgaaccgca
      541 cttcccaccc cgtctcccgg gcgagcagcg cgaagtcgag cacctcctcg tcgttggtcc
      601 cgcggatcac cacgcagttc agcttgatcg gcgcgaaccc ggcggcctcg gcggcggcca
      661 ggccggcgcg gacgcgggcg agcgcgtcgc gccgggtcat ctcggcgaac ctgtgcgcga
      721 gcagcgaatc gcaggacacg gtgacccgat cgaggccggc ggcccgcagc ggggcggcca
      781 ggcggtcgag cagcacgccg ttggtggtca tcgagaggtc gagcgggggg tcgatcgccc
      841 gcaggcgttg gacgacctcg accaggtccc gacgcgcggt cggctcgccg ccggtgagct
      901 tgacctcccg gaccccgagc gcgacgaaca gccggacgag ccgctcgatc tcggcggcgg
      961 cgaggtgctc gtcccgcgcc agccagggaa gcccctcggc cggcatgcag tacgtgcacc
     1021 gcaggttgca gcgatcggtg agcgagacgc gcaggtcgct ggcgacgcgg ccgaaggtgt
     1081 cgacgagagg aaggatcatc tggaccatcc taggccccca tgccggacct atcatcccgg
     1141 ggatggcacc ctcttcacgg cgggcgacgg tgctcacggt gagcgacggc gtccaccgcg
     1201 gggtccgcga ggaccgctcg ggcgcggccg tcacggcctc tctcgtccgc gctgggttcg
     1261 cggtgatgca ggagcgcgtc cccgacgagc gcgcggagat cgaggccgcg ctgcgacggc
     1321 tcgcggcgga ggccgagctg gtcgtcacga ccggcggaac gggcctcggt ccgcgcgacg
     1381 tgaccccgga ggccactcgc gcggtcgtcg accgcgaggc tcccggcctg gcagaggcca
     1441 tgcgggccgc cggccgggcg agcaccccgg ccgccgacct ctcgcgcggg atcgccggaa
     1501 cgctcggcgg cgccctggtc gtcaacctgc cggggagcga gcgcggggcc gtcgagagcc
     1561 tggaggccgt cctcccggtc ctcccccacg ccctcgacct gctggcagga cgcacggaac
     1621 acccggggga ccatccaccg gccacggccg gccgcgaggc gaacgagacg gcgtccgccg
     1681 acccggcggc cgaggcgctg cagcgcgcgg cggccggcga gccggtggtc ctggcgacgg
     1741 cggtgagcgt gaagggcgac ccgccatgcc ggcccggcca gaagctgctc ctcggccccg
     1801 gcgggccgct cgccggaacg ctcggctgcg cggagttcga cacgggggcc gcggccgatg
     1861 ccccggccgt gatctcggcc ggcgagccgg cgctgcgcca gtacagccac gagctcggca
     1921 cggtgcgggt ctacctcgag ccggtccgtc ggcgtccggc gctcgtcgtc ctcggcgcga
     1981 cgccggtcgc gctgtggctg ctgcgctggg gccgcgacct cgggttcgaa ccggcgctcg
     2041 tcgagtcgcg tgcggagcgg gtcacgccgg agcatcgcgc ggccgcagcg cgcgtcgccg
     2101 accggccgga ggccctcggg ccgctcgggg gggcgccggc cgtgcacacc gaccacgacg
     2161 cgccgctcgt cgccgagcac gtcaaggcgg tgatcgacgc gggggccggc ttcgtcgggg
     2221 tgatggggag cgctcgtcac ggcggcccgc acctcgagac gctccgggcc ctcggcgtcc
     2281 cgcccgatcg ggttgccgcc gtcgagacgc cggtcgggct cgacatcggt gcgcgcaccc
     2341 cgcaggagat cgcgctgtcg atcctcgccg ggatcgtcgc tcgccgcggc ggccgcgacg
     2401 gcggcccgct cgcccggcgg acgagatgag ggtccgggtc cggctgttcg gggcgctggc
     2461 cgagcgggcc ggcctccccg aggtcggcct cgacctcccg cccggcgcca ccgccgccga
     2521 cgcgtgccat gcggccctcc ggccgtaccc gactgccgcc ggcctggcgg agcacgtctc
     2581 gatcgccgtg aacctcgaag tggtcccggg accgcaccgg ctgaacgagg gtgacgaggt
     2641 tggcctcctc ccgccggtgg ctggcggcgc ggacggcccg cggatcctga ccggcctgcg
     2701 gcccggaggc cccgatccgg ccgaggcgct cgccgccgtc acggccgcgc gggcgggtgg
     2761 gatcgtcgcg ttcatcggga gcgtgcgcac cgaggacgac tggccggtcg aggcgctcga
     2821 gtacagcgcc tacgacgaga tggcggaccg ggtgctgaca gagatcgccg ccgaggtggc
     2881 cgagaagtgg ccgctcgagg ccgtggctat cgtccacgcc acggggcggc tgccggtcgg
     2941 cgccgtcacg ttcgtcgtgg cctgcgccgc cccgcatcgc gaggaggcct tcgccgcctg
     3001 ccggcacgcc gtcgaggagg tcaagcggcg cgccccgatc tggaagcgcg aggtcggccc
     3061 ccacggcgcg cgctgggtgg gcttgtgacc gccgacgaac tgctcccggt ggcgacggcg
     3121 ctcgacctgg cgctcgacgg gatcgggccc ctttcgccgg cagagctgag cctggaggcg
     3181 gcgtacgggt gcgcggcggc cgaggacgtc gtggccgacc ggttcctccc ctcgttctcg
     3241 tcctcgggga tggacgggtt cgccgtccgg gcgaacgacg tcgccttggc gagccccgag
     3301 tcgccggtgg agctccgcat caccgggcgg gtggcgatgg ggaaggcgcc cgacctggcc
     3361 gtgggctccg gcgaggcggt gcgcatcgcc accggcgccc cggtcccggc cggctccgac
     3421 ggcgtcgtgc cgatcgagct ctgcgaggtc ttcgccgacg cccgggtcct cgtccgccag
     3481 ggggtgccgc cgggccggta cctccgcccc gccgggcagg acgtccgccc cggcgaggtg
     3541 ctggttcgcg aggggcggcg gctcctcggg cctgagctcg gcctcttggc cctcgccggc
     3601 cgcgaccggg tccgcgtgcg gccgcgcccg cgggtggcga tcctctccac cggcgacgag
     3661 ctggtcccgc ccggcgcgat ggccggcttc ggccaggtgc acgacgcgaa cggccccacg
     3721 ctgttcggcg cggcgaagga agcgggcgcc gaaccacgac ttgccgggat cgtccccgac
     3781 gatgccgacg ccttccggga cgccctcggg cgcgccgcca cggacaccga cgtgctgctg
     3841 tcctcgggcg gcgtgtcggc cggagagctc gatgtggtca agcgaacgtt cgagggccgt
     3901 ggcgaggtcg ccttccaccg ggtggcgatg cagccgggga tgccgcaggc cttcggccgc
     3961 atcgacggca tcccgttctt cgggctcccg gggaacccgg tctcggtctt cgtctcgttc
     4021 gaggtcttcg tccggccggc catcctgcgc atgctcgggc gccggcggat cgtccgcccg
     4081 gcgatccgcg cgcgcgtggc cggcgagctc cgcggccagc cggagaagac cgtgttcgcc
     4141 cgcgtccggg cggcccgcaa cgagagcggc tggaccgcca cgtcgacggg aggcccacag
     4201 tcgaacctgc tggccacgct ctcgcaggcg aacgggctgg cggtgatccc gccgggcgtc
     4261 gagcgcctcg gccacggcga ggtctgccgg gtgatgctgt tccgcgaccc ggaggagacg
     4321 acgtgagcga ggaacgggcg gcgcggatgg tggacgtcgg cggcaaggag cccacggagc
     4381 gccgcgccgt ggccgagggc gcgctggcga cgacgcccga ggcctgcgcg cgactgaccg
     4441 agggcacgcc gaagggcaac ccgctcgagg cggcgcgcgt ggccggcctg ctggccgtga
     4501 agcgcacgcc cgacctgctc ccctactgcc acccggtcgc cgtgaccggg gcgtcggtcg
     4561 acgtggagcc cgaccccgcg accggccgca tccgcgtccg tgccaccgtc gaggcccgcg
     4621 accgcaccgg cgtcgagatg gaggccctga ccgccgtcgc cgtcgcagcc ctcagcctct
     4681 acgacaccgc caaaccctac gaccgcgccg caaggatcac cgacatccga ctggtctcga
     4741 aggaaggcgg caaatccggc cgctaccggg ccggctgagc gacggcgccg gccgctagtg
     4801 gaacgagtgg ccgcaggcgc aggagccctg ggccatcggg ttgtcgatcg tgaagccgct
     4861 ggcctccagg ctgtcgacga agtccacctt ggcctgcgac aggtacgggg cgctggtgcg
     4921 gtccacgacg atgcgcacgc cgtgctgctc ctcggccagg tcgccgtcgg cgagctggtc
     4981 gtcgagatac atcgcgtagc gcaggcccga gcatccgccg ggctgcacgg ccacgcggag
     5041 cgcgatgtcg gtgcgccctt cctggtcgag cagctccttg accttcgagg cggccatctc
     5101 ggtgagcgtg atcatcgttt ccgggtctcc ttggctgcga tccgacctcc aaagtatacc
     5161 cgcggggtcg ggaaccctcc tacccctccc ggaaggccgc cactcgcgcg gcggtgaccc
     5221 gcagcagggt ctgcgggtcg gtccggaaca cgccgtcggg ggtgccgctc gccgcccaga
     5281 cggcctcatg gcgcagcagg tcctcgtcca tcagcacggg cagcgggcgg ggatagccga
     5341 acggcggcgt cccgccgatg gcgaacccgg tcgccgcccg cgcctcgtcg gcgccggccc
     5401 gccggacgtc ctccgccccg aggagcgtcg ccaggcgcgt ggggtcggcc cggttggcac
     5461 cggaggtg
//
LOCUS       DASPWO010000079         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1115335, whole genome shotgun sequence.
ACCESSION   DASPWO010000079 DASPWO010000000
VERSION     DASPWO010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115335"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VEO00_01805"
     CDS             <1..542
                     /locus_tag="VEO00_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HYU56771.1"
                     /translation="DFADVKAVMTGAGSALMGIGHGNGDGRAEEAAKAAISSPLLESS
                     IEGAKGVLLSIAGPSDLALHEVNRAAEAITRVAHPDANIIFGAVIDDTLGEEVRVTVI
                     AAGFDKLGAGTDSRLARLLEAPTRAEGEDLEEEEEEELELAPSLLLDDEEEDLFKPAP
                     ATVTFDAEEDLDIPDFLKS"
     gene            764..1573
                     /locus_tag="VEO00_01810"
     CDS             764..1573
                     /locus_tag="VEO00_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HYU56772.1"
                     /translation="MPSPSTRSTRASTDLHRRTVRDVDFLVHRRLERRGVLAAFTERT
                     GGLSEPPFDSLNLSLATGDDSSRVRANRERVAEALGTGAFALGQQVHGARSARVGRTR
                     AGAGFADRNDALPGTDSLSTAARGVSLAVLTADCVPVVLADRARVAVAHVGWRGLVAG
                     IVRRALGAFADPAAVTAVLGPAIGPCHYEVGPEVALAAASATGSGARTERRGGALYLD
                     LPATVAGALRDLGVRAPEAAGICTACERERFFSHRRDGPATGRQAAIVMRL"
     gene            1661..2314
                     /locus_tag="VEO00_01815"
     CDS             1661..2314
                     /locus_tag="VEO00_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HYU56773.1"
                     /translation="MRERLVRVRAAIAAACGRVGRDPATVGVVVVTKGFPAEVARAAF
                     EAGAEDLGENYVQELRAKREAAPGARWHFLGTVQANKARAIAECHLVHGLEPGHGATR
                     LGRVGAGRGTPVRCLAEVDFTGRRQGASPDGLPAFLETLRSDPGLDLRGLMTVAPPGS
                     AEEARRAFADLRALRDRLGPDLPELSMGMSGDYPVAVEEGATMVRIGTAVLGERRDD"
     gene            2326..2817
                     /gene="sepF"
                     /locus_tag="VEO00_01820"
     CDS             2326..2817
                     /gene="sepF"
                     /locus_tag="VEO00_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HYU56774.1"
                     /translation="MPGVWKKTLQYLGLVEDEDYEELGEVVEAPVATREPATVRRLRS
                     EPTTSVRESSTPEAIIRTIPQTRPAAANSIHRAEPKAFNEARELGERFKQGIPVIMNL
                     QGTEDHIARRLVDFASGLVFGLEGKIEMVASRVYLLTPENMEVSAEERERLRTEGGFY
                     NQL"
     gene            2857..>3109
                     /locus_tag="VEO00_01825"
     CDS             2857..>3109
                     /locus_tag="VEO00_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HYU56775.1"
                     /translation="MVTIVCYAISVYTIVLFIRIIWSWFRVPSSGPGRTIFEVIYDLT
                     EPVLRPGPHRRHQTADQSEHRLGQIVDHLEDRAPRAGGRH"
BASE COUNT          431 a         1104 c         1175 g          399 t
ORIGIN      
        1 tggacttcgc cgacgtgaag gccgtgatga ccggcgccgg gtcggcgctg atgggcatcg
       61 gccacggcaa cggcgacggc cgggcggagg aggccgcgaa ggcggcgatc tcctcgcccc
      121 tgctcgaatc ctcgatcgag ggggcgaagg gcgtgttgct gtcgatcgcc ggcccgtcgg
      181 acctcgcgct gcacgaggtg aaccgcgcgg ccgaggcgat cacccgcgtc gcccaccccg
      241 acgcgaacat catcttcggc gcggtgatcg acgacacgct gggcgaggag gtccgggtca
      301 cggtcattgc cgccgggttc gacaagctcg gcgccgggac cgactcgcgg ctcgcccgcc
      361 tgctcgaggc cccgacccgc gccgagggcg aggacctgga ggaggaggaa gaggaggagc
      421 tcgagctggc gccctcgctc ctgctcgatg acgaggagga ggacctcttc aagccggccc
      481 ccgcgacggt gaccttcgac gccgaggagg acctcgacat ccccgacttc ctgaagagct
      541 gaagggctga gagccgacgg cagagagacg agaccgcgag gacgacgaca cgacgacgcg
      601 agacgaacgc agcgcgggcg acgacccgcc gacgaacctc cgggctcggg cacggcccgg
      661 agcgggcgtg ggggcgggga gggccggagc aggcgccctt cccccgcctt gacgtaaggt
      721 cctccccgtc cccacccacc tcaccgcgcg gcctcccccc ggaatgccct ctccctccac
      781 tcgctccacc cgcgcctcga ccgacctcca ccggcgcacg gtccgcgacg tcgatttcct
      841 ggtccaccgg cgcctcgagc gccgcggcgt gctggcggcg ttcaccgagc gcaccggagg
      901 gctgagcgag cccccgttcg actcgctgaa cctgtcgctc gcgaccggcg acgactccag
      961 ccgcgtgcga gccaaccgcg agcgcgtcgc ggaggccctc ggcacgggag ccttcgcact
     1021 ggggcagcag gtgcacggcg cgcggtcggc ccgcgtcgga cgaacgcgcg ccggggcggg
     1081 gttcgccgac cgtaacgacg cactccccgg gaccgactcg ctctccaccg cggcgcgggg
     1141 cgtgtccctg gcggtgctga ccgccgactg cgtgccggtg gtcctcgccg accgcgcccg
     1201 tgtcgcggtg gcccacgtcg gctggcgggg attggtagcc gggatcgtcc gccgcgccct
     1261 cggggcgttc gccgacccgg cggccgtcac cgccgtcctc ggaccggcga tcggaccgtg
     1321 ccactacgag gtcggccccg aggtggcgct cgccgcggcc tcggccaccg ggagcggtgc
     1381 ccgaaccgag cgtcgcggcg gcgcgctgta cctcgacctg ccggccacgg tggccggcgc
     1441 gctccgcgac ctcggcgtgc gcgctcccga ggcggccggg atctgcacgg cctgcgagcg
     1501 cgagcgcttc ttctcccacc gccgggacgg gccggcgacg ggccggcagg ccgccatcgt
     1561 gatgcgcctg tgaatcgcag gtgacgccgg atatgatgcg tgaaacgttg ctcactgttg
     1621 ttcacaagtg tcaactccag tgataccctc ccccgaggtc gtgcgggagc gcctggtccg
     1681 ggtccgcgcc gcgatcgcgg cggcgtgcgg ccgggtcggc cgcgatcccg ccaccgtcgg
     1741 ggtggtggtg gtgaccaagg ggttcccggc cgaggtggcg cgcgccgcct tcgaggccgg
     1801 ggccgaggac ctcggggaga actacgtgca ggagctgcgg gcgaagcggg aggcggcgcc
     1861 gggggcgcgc tggcacttcc tgggcacggt gcaggcgaac aaggcccggg cgatcgccga
     1921 gtgccacctg gtgcacggcc tggagccggg acacggggcg acgcggctcg gccgggtcgg
     1981 cgccgggcga gggacgccgg tgcggtgcct ggcggaggtc gacttcaccg gccggcgcca
     2041 gggcgcctcg cccgatgggc tgccggcgtt cctggagacg ctccgttccg accccgggct
     2101 cgacctccgg ggcctgatga cggtcgcccc tccggggtcg gccgaggagg cgcgccgggc
     2161 gttcgccgac ctgcgggcgc tgcgcgatcg cctcggcccc gacctgcccg agctgtcgat
     2221 gggaatgtcc ggcgactacc cggtcgccgt ggaagaaggc gctacgatgg tgcggatcgg
     2281 cacggcggtc ctcggtgagc gccgcgacga ctgaaggggg ccgcgatgcc aggcgtgtgg
     2341 aagaagacgc tccagtacct cggcctggtc gaggacgagg actacgagga gctcggcgag
     2401 gtcgtggaag ctcccgttgc gacgcgcgag ccagccacgg tgcgccggtt gcgctccgag
     2461 ccgacgacca gcgtgcggga gtcgtccacg ccggaggcga tcatccggac gatcccgcag
     2521 acgcggccgg cggcggccaa ctcgatccat cgcgcggagc cgaaggcgtt caacgaggcg
     2581 cgcgagctcg gcgagcggtt caagcagggg atccccgtga tcatgaacct gcaggggacc
     2641 gaggaccaca tcgcccgacg gctggtggac ttcgcgtcgg ggctggtgtt cggcctggag
     2701 ggcaagatcg agatggtggc gagccgcgtg tacctgctga cgccggagaa catggaggtg
     2761 tcggcggagg agcgcgaacg cctgcggacc gagggcggct tctacaacca gctgtagcga
     2821 tgcgcagcgc ccgacgcggc gcccggaggc cgatgcgtgg tcacgatcgt ctgctacgcg
     2881 atctccgtct acacgatcgt cctgttcatc cgcatcatct ggtcgtggtt ccgggtgccg
     2941 tcctccggcc cggggcgcac gatcttcgag gtgatctacg atctgaccga gccggtgctc
     3001 cgacccggtc cgcatcgccg gcaccagacc gcggaccagt cggagcaccg gctcggtcag
     3061 atcgtagatc acctcgaaga tcgtgcgccc cgggccggag gacggcacc
//
LOCUS       DASPWO010000080         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_524318, whole genome shotgun sequence.
ACCESSION   DASPWO010000080 DASPWO010000000
VERSION     DASPWO010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524318"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VEO00_01830"
     CDS             complement(<1..739)
                     /locus_tag="VEO00_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015619180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYU56776.1"
                     /translation="MITPRFVDAILTGPSSGPSPDGDGRLQGVEDEALDAYSRVVTTV
                     AERLIPSVASLRVMRRVPGGRRAEGAGSAVALTPDGFLVTSAHVVQGSDAGTASFVDG
                     RELEFRVIGTDPLSDLAVVRAQGSDLVAAELGDADRLRVGQLVVAIGNPLGFAGSVTT
                     GVVSALGRSLPTRAGSTTRIVENVIQTDAALNPGNSGGALSDGLGRVVGVNTAVAGIG
                     LGLAVPINATTRRIIAALMTDGRFRRAY"
     gene            817..2157
                     /locus_tag="VEO00_01835"
     CDS             817..2157
                     /locus_tag="VEO00_01835"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYU56777.1"
                     /translation="MLTTLETFFARLREAGVPISVHEALDAARAAGAVDATGRDVLRA
                     ALAATIVKDDAHRTAFDAVFDVTFAARSVPAEGEEEGEPPSALAERLRGELEEAVRRG
                     DLEAVEELARRAARVFAGVGDRQSGPGYFSYRAAQALGADEVWRRLLGGIGAGAPLER
                     ALEEAGIAVAQAAFDDALRREVLRLLVQDRGPETVNRWVARPLPENVEFVTATRDERE
                     ALTKAVRPLARRLATRLARRKRHGPHGRLDVRRTVRASMSTGGVPFDPRFRRRHPHRP
                     DLFVLCDVSGSVAAFSRFTLQFVHALQSQFRAVRSFVFVDTLDEVTEDFRGGEFAPSL
                     AAMVQRARVVWGDGHSDYGSSLKRFERLAGDALTKRSVVLVLGDARNNYRDPGDAAVA
                     RIAERARRVWWLNPEPRRWWDTGDSVAARYVPHLTGMHECRNLRQLEAFVERVV"
     gene            2200..>2417
                     /locus_tag="VEO00_01840"
     CDS             2200..>2417
                     /locus_tag="VEO00_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HYU56778.1"
                     /translation="MARRVGVMGGTFDPIHIGHLVTAEEALSQFSLDEVLFVPTGQPW
                     MKSERAVTASEHRYLMTVIATSSNPRFWV"
BASE COUNT          296 a          876 c          929 g          316 t
ORIGIN      
        1 ggtacgcccg gcggaaccgc ccgtcggtca tgagggcggc gatgatccgc cgggtggtgg
       61 cattgatcgg caccgcgagc cccagcccga tcccggccac cgcggtgttc accccgacca
      121 cgcggccgag gccgtcggag agcgcgccgc cggagttccc cgggttcagc gcggcgtcgg
      181 tctggatgac gttctcgacg atgcgcgtcg tggagccggc acgggtcggg agcgagcgtc
      241 ccagcgcgct gaccacgccg gtggtgacgc tgcccgcgaa cccgagcggg ttgccgatcg
      301 ccaccaccag ctgcccgacg cgcaagcggt cggcgtcgcc gagctcggcc gccacgaggt
      361 ccgagccctg ggcgcggacg accgcgaggt ccgagagcgg gtcggtgccg atcacccgga
      421 actccagctc gcggccgtcg acgaacgagg ccgtgccggc gtcggacccc tgcacgacgt
      481 gcgccgaggt gaccaggaac ccgtccgggg tcagcgcgac ggccgaaccg gccccctcgg
      541 cccggcgccc gccgggcacg cggcgcatca cgcgcaggct ggcgaccgag gggatcaggc
      601 gctcggcgac ggtggtcacg acccgggagt aggcgtcgag ggcttcgtcc tcgacgccct
      661 gcaggcgccc gtcgccgtcc ggcgagggcc cgctcgaggg cccggtgagg atggcgtcca
      721 cgaagcgggg tgtaatcatg tttgcacggt agcaccggtc cccgaaacgc aggctccccg
      781 ccggccgcga gacaatcccg tcgtgaggcg ctcgcgatgc tgaccaccct ggagaccttc
      841 ttcgcccggc tgcgcgaggc gggggtgccc atctccgtgc acgaggccct cgacgccgcg
      901 cgcgccgcgg gcgcggtgga cgccaccggc cgcgacgtgc tgcgggccgc cctggcggcg
      961 acgatcgtca aggacgacgc ccaccggacg gcgttcgacg cggtcttcga cgtgacgttc
     1021 gccgcgcggt cggttccggc ggaaggcgag gaggagggcg agcccccgtc ggcgctggca
     1081 gagcggctgc gcggcgagct cgaggaggcc gttcgccgcg gtgacctgga ggccgtcgag
     1141 gagctggccc gtcgcgcggc gcgcgtgttc gccggcgtgg gggaccgcca gtccgggccg
     1201 ggctacttct cgtaccgcgc ggcgcaggcg ctcggggccg acgaggtctg gcggcgcctg
     1261 ctcggcggga tcggcgccgg cgcgccgctc gagcgggcgc tcgaggaggc cgggatcgcg
     1321 gtggcgcagg ccgcgttcga cgacgccctg cgccgcgagg tgctgcgcct gctcgtccag
     1381 gaccgcggcc cggagaccgt caaccgctgg gtggcccgcc cgctgccgga gaacgtggag
     1441 ttcgtcaccg cgacgcgtga cgagcgcgag gccctgacga aggcggtgcg accgctggcg
     1501 cggcggttgg cgacgcgcct cgcccggcgc aagcgccacg ggccgcacgg ccggctcgac
     1561 gtgcggcgca ccgttcgcgc ctcgatgtcc accggcggcg tgccgttcga cccgcggttc
     1621 cgccgccgcc acccgcaccg cccggacctg ttcgtgctgt gtgacgtctc ggggtcggtg
     1681 gcggcgttct ctcgcttcac cctgcagttc gtccacgccc tgcagagcca gtttcgcgcg
     1741 gtgcgctcgt tcgtcttcgt cgacacgctc gacgaggtga ccgaggactt ccgcgggggc
     1801 gagttcgcgc cgtcgctggc ggcgatggtg cagcgggcgc gggtggtgtg gggcgacggg
     1861 cactcggact acggctcgtc gctgaagcgg ttcgaacgac tggccggcga cgcgctgacg
     1921 aagcggtcgg tggtgctggt gctcggcgac gcgcggaaca actaccgcga ccccggcgac
     1981 gcggccgtgg cccgcatcgc cgagcgcgcc cgccgggtct ggtggctgaa ccccgagccc
     2041 cgccgctggt gggacacggg cgactcggtc gccgcgcgct acgtcccgca cctgaccggc
     2101 atgcacgagt gccgcaacct ccgccagctc gaggcgttcg tcgaacgggt ggtctgaggt
     2161 tccgggccgg cgggggagcg gcccgtagaa tcgacggcga tggcgcggcg cgtcggggtg
     2221 atgggcggga cgttcgaccc gatccacatc ggccacttgg tgaccgccga agaggccctc
     2281 tcccagttct cgctggacga ggtgctgttc gtccccaccg gccagccgtg gatgaagtcc
     2341 gagcgggccg tcaccgcatc cgagcaccgc tatctcatga ccgtcatcgc cacctccagc
     2401 aacccccggt tctgggt
//
LOCUS       DASPWO010000081         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_183689, whole genome shotgun sequence.
ACCESSION   DASPWO010000081 DASPWO010000000
VERSION     DASPWO010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183689"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..850)
                     /locus_tag="VEO00_01845"
     CDS             complement(302..850)
                     /locus_tag="VEO00_01845"
                     /inference="COORDINATES: protein motif:HMM:NF044877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56779.1"
                     /translation="MVPNPARRDADRELRDARRRLGECEAAEGRASVGGRRPSKEILQ
                     AFTGARAEVERLAAAAKAIPAKAPLGEVRPDAVRLAPERKRIHDAVRMATYNAESALA
                     RLLAPHYARAEDEARSLLREAFRAPADLQVIGDELHVRLSPLSAPRRTGAITGLCEEL
                     TATKTLYPGTDLTLVYSVKGQG"
     gene            complement(949..2046)
                     /locus_tag="VEO00_01850"
                     /pseudo
     CDS             complement(949..2046)
                     /locus_tag="VEO00_01850"
                     /inference="COORDINATES: protein motif:HMM:NF044877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          275 a          750 c          848 g          322 t
ORIGIN      
        1 ggacgcccgg tagtgacgtg accttccggc gcggtggtcg tatcgtcacc gtccacgtaa
       61 cgcaactgtc accttccgtc gttgcggcat cggcgacgtc tccgccccag caagtcatcg
      121 ttgccaccag gaccgctccg aacacgtgtc gcggattgcg ccccgaccgt cccaatcggc
      181 ggccctgccg taggtatcgt cgcccggccg ggtcgtaaac ccagacactg gcgaagcctc
      241 acggggtgcc cgcccatcag gaatccagac ctcctgacat agagcgatca tctctgcgga
      301 gtcacccttg gcccttgacc gagtagacga gggtgaggtc tgttcccggg tagagggtct
      361 tggtggcggt gagttcctcg cacagcccgg tgatcgcccc ggttcgtcgg ggtgctgaca
      421 gcggcgagag gcgcacgtgg agctcgtcgc cgatcacttg cagatcggcc ggggctcgga
      481 acgcttcgcg cagcaggctg cgggcctcgt cctccgcccg cgcgtagtgg ggggcgagga
      541 ggcgggcgag ggcggactcg gcgttgtagg tcgccatgcg gacagcgtcg tggatgcgct
      601 tgcgctcggg ggcgaggcgc acggcgtcgg gccgcacctc gccgaggggt gctttggcgg
      661 ggatggcctt ggccgcggcc gccagtcgct ccacctcggc tcgtgcgccg gtgaaggcct
      721 ggaggatctc cttgctcggt cggcggccgc cgaccgaggc cctgccctcg gccgcttcgc
      781 actcgccgag gcggcgccgt gcgtcgcgaa gctcccggtc ggcgtcgcgc cgggcggggt
      841 tgggcaccat gcgggcgggg tcgtcgtcgg cggtggcgta gctgtcgagg cgtcgaggcc
      901 gtagtgggcg cgcatgtagc ggaagaagtt ctcctggccc cagcgcccga acatggcgtg
      961 ggccaccggg gccgggtcgg cgtcgtctcg ggtggtgagg acctgggtct ggtggccggt
     1021 cgcctcgtcg aggcgggtga tctgccggca ggagaagcgt cgccgtccgc tgtcgtaggc
     1081 gatgcgcacc ttgcggtcgg cgagcagata gtcctgggcg cgtccggcgt cgtcagtgaa
     1141 gcggcgcccg acgaaggccg agcgcggctc tctgggggcg gggcccttgc ggtaggtgag
     1201 gatgtcgaac ccggcggcgt gcagttcggc aaacagcttc ggggaccagc cgccgcggtc
     1261 gaagcagatc gtcgggcggg catcggggcc gaccagctcg cggatcttgg cggtgaccat
     1321 ccgaagctcg ccggcgaggc tggcgcccgg cggtgcgccc cacaccagca ggccgtcgcc
     1381 gttgcggtcg cagacccagg tgtcgacctc ggcgcccatg gccaggcgga tgcgcgccac
     1441 gtgcgccttg gggacctccc gggcgccgtg ataggcgcgc acgtggccgt cgacgtagaa
     1501 gatccccgtg gctccggcgt gggtgtcgag gtgacgacgg gcgagcgcgt cgatgagccg
     1561 gtcggcacgc ccgagccgag ccagctcctc catgcgtcgg cgcatcgtct tgacctcggg
     1621 cgcccggtcg agcccgagta gtcgtcccac gtcggtcggg tcgatgcgtg tcagcccctc
     1681 ggcgcggggt tcgccgatca agcacgcgaa cacgattgcc atcacgagcg agcgcagccc
     1741 gtagaaggca gcccgcccgg tggtgtagac ccgcgctgcc gcgtcgagca ggcccgtcgc
     1801 ggcgagtgcc ggcaagatga tgagcgcgcc ggcgaggggc agcgaggcac cctcgcagat
     1861 gaccggtggc gcctcggtga tgagcccggc ccgggccgcc tggcgctcgg ccgagcggtc
     1921 caccgggcgg gcgaggggct cgagcccggg cccgccggcg ggctcgaccg ccttcggcgc
     1981 gggcaccgcc gagtcggcca aggcccggcg cacggtgtcg gtcgacaccc cggcgcgccc
     2041 agccacggcg agcaacgcca gcccctcgga gcgcagcgct cggatctcgg cccgcttggc
     2101 gtcggtgagc ttcgagggtc ccttcgggcc cggccggcgc ccggccagcc ccgcggtgcc
     2161 gttggccttg tactcggcgc gccagatgat gaggg
//
LOCUS       DASPWO010000082         4865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_524615, whole genome shotgun sequence.
ACCESSION   DASPWO010000082 DASPWO010000000
VERSION     DASPWO010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524615"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VEO00_01855"
     CDS             <1..603
                     /locus_tag="VEO00_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56780.1"
                     /translation="YPYDIVVRPGGGVAVAGTTYNSDRRVAVAALTANGSLDPSFGDG
                     GRFTYGVGGEDAEGFALAQKQGKLLVAGRQGGSGDTDGLVLRLTVGGSLDPLFGDGGV
                     TRVPLSPGEDVFNGIAVLPGGRFVAAGNTDEVNLGPTAIVRLTAGGDLDPTFGQGGVS
                     TLTELASIEDVVVDGTGRPVVAGRDSLTVPFNMAAARFLV"
     gene            656..1924
                     /locus_tag="VEO00_01860"
     CDS             656..1924
                     /locus_tag="VEO00_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56781.1"
                     /translation="MTRHRTQLVLVAGILLAALASGPAAADPGELDPTYRNGGVSVVP
                     FDGVSSLAQAMARMPDGGVVLAGQAGMGATELALARLRLDGRPHKGFGSSGRVVADSG
                     SPDEFFDAVAVQGSRILAVENDDSGTPDADLVVMAFRLDGAPDTSFGVGGTASVDFGG
                     DDFGQAIAVDGKGRVLVAAIVYPPQGGPSAIGVVRLTPNGAVDPTFGNNGRRRVAFPD
                     GFSFPTDIVVRPGGVAVAGVASISIGRKVAVAALTPNGALDTSFSGDGRFTYGEDDER
                     TEAFAIVRRQGELLVAGTQGEYGDTDGLVLRLTVDGELDPLFGDGGVTRVPFTPGEDV
                     FEGIAVLPDGRLVAAGTTDDASGPPVIVRLTPGGDLDPTFGQGGVSTLPEFAFIEDVV
                     VDGTGRPVVAGAAEDSNVAPFNMAAARFLV"
     gene            1944..3224
                     /locus_tag="VEO00_01865"
     CDS             1944..3224
                     /locus_tag="VEO00_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56782.1"
                     /translation="MNRRTALTLLAALLLVALAAFPAAAAPGDLDPTFAGGGVRLIQF
                     GANDSQAFDLVVMLDNGAVLAGRAFLPGGVEPALARVDRDGRLVKSFGVNGRMVEDGG
                     GVADIFEAVARQGSKLLAVAVDSTNNTPTADFMVFRFNGNGTLDTSFGVGGVARTDFG
                     GADRASDLVVLPDGRFVVVGRAIADGTATFGAARFTPGGAPDTTFGNNGRRRIAFPGA
                     PSGGATAVALRPGGGVVIVGSMQDINVDAGAWAIAALTPSGGIDTSFSGDGRVRLPFG
                     DNSDGATSVAVRQGKILVGGFVFGEDTAQDFALVRFQPDGALDPLYGEGGVAILPSGD
                     EPDVPNGLAIASDGRAYLVGGTADGNELGILARVTPGGDPDPSFGDGGFVSFLYNEYA
                     FGSAVGLDSAERAVVAGEGGVKGTPDMLAARFLT"
     gene            3328..4014
                     /gene="ftsE"
                     /locus_tag="VEO00_01870"
     CDS             3328..4014
                     /gene="ftsE"
                     /locus_tag="VEO00_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HYU56783.1"
                     /translation="MIRFDHVSKIYKEDAAALDDVTLEIEKGEFVFVVGPSGSGKSTF
                     IRLVLKEEEPTKGSIYVAGKNIKTLSPWKVPYLRRNIGTVFQDFKLLPDKTAFDNVAF
                     ALEVIGKPRHTIDQRVPEIMQLVGLGDRLAALPDELSGGEQQRVSIARAFVNRPLILL
                     ADEPTGNLDPATSVDIMKLLDRINRTGTTVIMATHDNAIVDAMRRRVVELDNGQVRRD
                     QARGVYGYGA"
     gene            4004..>4865
                     /gene="ftsX"
                     /locus_tag="VEO00_01875"
     CDS             4004..>4865
                     /gene="ftsX"
                     /locus_tag="VEO00_01875"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HYU56784.1"
                     /translation="MARRLGYFFRETTGGLRRNGLIAFAAISTVFVSLFLLGAALLVG
                     REVDLIAESTGSKVEVSVFLKDSISQSQIDRLERLISDMPEVASVEFESKEAAYERFK
                     RIFANQEALVQNVTADAMPQSFRIKLNDPEKFEVISARLANEPGIDQIIDQREILKRL
                     FAVTRVLRVGAFAAGIIMLLSAAALIGNTVRMAVFARRKEIGIMKLVGATNWFIRVPF
                     LIEGMVEGLLGAGAAILGLLVIKVAFIDPLRNNIGFLPLIGTSDVLFAVPWLIAAGVL
                     VAVLASLFAMR"
BASE COUNT          678 a         1671 c         1784 g          732 t
ORIGIN      
        1 tacccgtacg acatcgtcgt ccgccccgga ggaggagtgg cggtcgcggg gaccacctac
       61 aacagcgacc gcagggtcgc cgtggcggcg ctcacggcga acgggtcgct ggacccctcc
      121 ttcggcgacg gtggccgctt cacctacggc gtgggcggcg aggacgccga aggtttcgcg
      181 ctcgcgcaga agcagggcaa gctgctggtg gccggcaggc agggcgggtc cggcgacacg
      241 gacggcctgg tgctccggct gaccgtgggc ggctcgctcg acccgctgtt cggcgacggg
      301 ggcgtgaccc gcgtgccgct cagccccggg gaggacgtgt tcaacgggat cgccgtcctg
      361 cccggcggcc ggttcgtggc ggcgggcaac acggacgagg tgaacctcgg cccgacggcg
      421 atcgtgcggc tcaccgccgg cggcgacctg gacccgacgt tcggccaggg cggcgtgtcg
      481 acgctcaccg agctcgcgag catcgaggac gtcgtcgtcg acggaaccgg ccgtcccgtg
      541 gtcgccggcc gggacagcct gacggtcccg ttcaacatgg ccgccgcccg gttcctggtg
      601 tgaacgaccg aggtcgagcc aacgaggctg agcgaacgag aaggaggaga tcgcgatgac
      661 ccgtcaccga acccagctcg tgctcgtggc gggcatcctg ctcgccgcgc tggcctccgg
      721 tccggcggcc gccgaccccg gcgaactcga cccgacctac cgaaacgggg gcgtcagcgt
      781 cgtcccgttc gacggggtgt cgtcgttggc ccaggccatg gcgcgcatgc cggacggggg
      841 cgtggtgctt gccggccagg cgggcatggg cgccacggag ctggcgctgg cccgcctgcg
      901 gctcgacggc cgcccgcaca agggcttcgg ctcgagcggt cgcgtggtcg cggactccgg
      961 atccccggac gagttcttcg acgccgtggc cgtgcagggg agtcgcatcc tggcggtcga
     1021 gaacgacgac tcggggactc cggacgcgga cctggtggtc atggcgttcc gcctggacgg
     1081 cgcccccgac acctccttcg gcgtcggcgg gaccgcgtcg gtggacttcg gcggcgacga
     1141 cttcgggcaa gcgatcgccg tcgacggcaa gggtcgggtc ctcgtggccg ccatcgtgta
     1201 tccgccgcag ggcggtccga gtgcgatcgg cgtcgtgcgg ctgaccccga acggggcggt
     1261 cgacccaacg ttcggcaaca acggccgccg gcgggtcgcg ttcccggacg gcttctcgtt
     1321 cccgaccgac atcgtcgtcc ggccgggagg ggtggccgtc gcgggcgtcg cctccatctc
     1381 gatcgggcgc aaggtcgccg tggcggcgct cacgccgaac ggggcgctgg acacgtcctt
     1441 ctcgggcgac ggccggttca catacggcga ggacgacgag cgcaccgaag cgttcgcgat
     1501 cgtccggagg cagggcgagc tgctcgtggc cggcacgcag ggcgagtacg gcgacaccga
     1561 cggactggtg ctgcggctga ccgtggacgg cgagctcgac cctctcttcg gcgacggggg
     1621 tgtgacccgc gtgccgttca cccccggaga ggacgtgttc gagggcatcg ccgttctccc
     1681 ggatggccgc ctcgtggcgg ccggaaccac ggacgacgcg agcggacctc cggtaatcgt
     1741 gcggctcacg cctggcggcg acctcgaccc gacgtttggg caaggcggtg tgtcgacgct
     1801 tcccgagttc gcgttcatcg aggacgtcgt cgtcgacggc accggccgcc cggtggtcgc
     1861 cggcgccgcc gaggacagca acgtggcccc gttcaacatg gccgccgccc ggttcctggt
     1921 gtgaccaggg gagggagact ccgatgaatc gcaggacggc gctcacactg ctggcggccc
     1981 tgctgctcgt ggcgctcgcg gccttcccgg cggccgccgc gccgggtgac ctcgacccga
     2041 cgttcgccgg aggaggggtc cggctgatcc agttcggggc gaacgactcg caggcgttcg
     2101 acctggtcgt catgctcgac aacggggcgg tgctggcggg ccgcgccttc ctgcccggcg
     2161 gggtcgagcc ggcgctggcg cgggtcgacc gtgacgggcg cctggtgaag tcgttcggcg
     2221 tgaacggccg catggtggag gacggcggcg gggtggcgga catcttcgag gcggtcgctc
     2281 ggcagggatc gaagctgctg gccgtcgcgg tggactcgac gaacaacacg ccgacggccg
     2341 acttcatggt cttccgcttc aacggcaacg ggacgctcga cacctccttc ggggtcggcg
     2401 gcgtcgcccg gacggacttc ggcggggcgg accgcgcgag cgacctggtc gtcctccccg
     2461 acggacgctt cgtcgtggtc ggccgggcca tcgcggacgg aaccgcgacg ttcggcgccg
     2521 cccggttcac gcccggcggc gctccggaca cgacgttcgg gaacaacgga cgccggcgca
     2581 tcgcgttccc cggcgctccg tcgggtggcg ccaccgcggt cgcgctgcgg ccgggcgggg
     2641 gcgtcgtgat cgtcgggagc atgcaggaca tcaacgtgga cgccggcgcc tgggcgatcg
     2701 ctgcgctcac cccctcgggc gggatcgaca cgtcgttctc gggcgacgga cgggtccggc
     2761 tgccgttcgg cgacaacagc gacggcgcga cgagcgtcgc ggtccggcag ggcaagatcc
     2821 tggtcggcgg attcgtgttc ggcgaggaca cggcccagga cttcgccctc gtgcgcttcc
     2881 agccggacgg cgccctcgat ccgctgtatg gggagggcgg cgtggcgatc ctcccgagcg
     2941 gggacgaacc ggacgtcccg aacggcctgg ccatcgcctc cgacgggcgt gcctacctgg
     3001 tcggcgggac cgccgacggc aacgagctgg gcatcctggc ccgggtgacc cccggcggcg
     3061 accccgaccc gagcttcggc gacgggggct tcgtctcgtt cctgtacaac gagtacgcgt
     3121 tcggctccgc ggtcggcctg gactcggcgg agcgagccgt cgtcgccggt gagggaggtg
     3181 tgaagggaac tccggacatg ctggccgccc ggttcttgac ctgagccgga ccgccccgga
     3241 ggtggttccg ttcggctggg gcgtctggta gtctgaccgc cccggaaccc cgaaccagac
     3301 caaccgcgga aaggctctgg gctgcccatg atcaggttcg accatgtcag caagatctac
     3361 aaggaggacg ccgccgccct cgacgacgtc acgctcgaga tcgagaaggg cgagttcgtc
     3421 ttcgtcgtcg gcccgtccgg ctcgggcaag agcaccttca tccggctggt cctgaaggag
     3481 gaggagccga cgaagggctc gatctacgtg gccggcaaga acatcaagac cctctccccg
     3541 tggaaggtgc cgtacctgcg ccggaacatc ggcaccgtgt tccaggactt caagctcctt
     3601 ccggacaaga ccgcgttcga caacgtggcg ttcgcgctgg aggtgatcgg caagcctcgg
     3661 cacaccatcg accagcgcgt gcccgagatc atgcagctcg tggggctggg agaccggctc
     3721 gcggcgcttc ccgacgagct ctccggcggc gagcagcagc gcgtctcgat cgctcgggca
     3781 ttcgtcaacc ggccgctgat cctgctggcc gacgagccga ccgggaacct cgatccggcg
     3841 acgtccgtgg acatcatgaa gctgctcgac cggatcaacc gcactgggac gaccgtgatc
     3901 atggccaccc acgacaacgc gatcgtggac gccatgcgcc ggcgggtcgt cgagctcgac
     3961 aacggccagg tccgccggga ccaggcccgt ggggtctacg ggtatggcgc gtaggctcgg
     4021 ctacttcttc cgcgagacca ccggcggcct ccgccgcaac ggcctgatcg ccttcgccgc
     4081 gatctccacc gtgttcgtct cgctcttcct gctcggcgcg gcgctcctcg tcggccgcga
     4141 ggtggacctg atcgccgagt ccaccggctc caaggtggag gtctcggtgt tcctgaagga
     4201 ctcgatcagc cagtcgcaga tcgaccggct ggagcgcctg atcagcgaca tgcccgaggt
     4261 cgcctcggtg gagttcgagt cgaaggaggc ggcctacgag cgcttcaagc ggatcttcgc
     4321 caaccaggaa gcgctcgtgc agaacgtcac cgcggacgcc atgccgcagt cgttccggat
     4381 caagctgaac gatccggaga agttcgaggt gatctcggct cgcctggcga acgagccggg
     4441 gatcgaccag atcatcgacc agcgcgagat cctgaagcgc ctcttcgcgg tgacgcgcgt
     4501 gcttcgcgtc ggcgccttcg cggcgggcat catcatgctg ctcagcgcgg cggccttgat
     4561 cgggaacacc gtgcgcatgg ccgtcttcgc ccgccgcaag gagatcggga tcatgaagct
     4621 ggtcggcgcg acgaactggt tcatccgcgt gccgttcctg atcgagggga tggtcgaggg
     4681 cctgctcggc gccggcgccg cgatcctcgg cctgctggtg atcaaggtgg cgttcatcga
     4741 cccgctgcgc aacaacatcg gcttcctccc cctgatcggc acctccgacg tgctgttcgc
     4801 cgtcccgtgg ctgatcgcgg cgggcgtcct ggtggccgtg ctggcgagcc tgttcgccat
     4861 gcgcc
//
LOCUS       DASPWO010000083         5699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_525167, whole genome shotgun sequence.
ACCESSION   DASPWO010000083 DASPWO010000000
VERSION     DASPWO010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525167"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..587
                     /gene="hpt"
                     /locus_tag="VEO00_01880"
     CDS             3..587
                     /gene="hpt"
                     /locus_tag="VEO00_01880"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HYU56785.1"
                     /translation="MPVDAARLAAHVSEYESDIERILITEAEIQARLAELGAQITEDY
                     AGRDPLLVGVLKGAFMLMADLSRHIHLPLEFDFMAVSSYGSATRTSGVVRILKDLDHE
                     IQGRDVLLVEDIIDSGLTLNYLLKNLRSRKPGTLEVCALLHKTEMQRVPLDIRYTGFE
                     IPPEFVIGYGLDYAERFRNLPYVATLKREAYAAG"
     gene            complement(602..1552)
                     /locus_tag="VEO00_01885"
     CDS             complement(602..1552)
                     /locus_tag="VEO00_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56786.1"
                     /translation="MSRRAAILLSLLVALTAGGNAAADTAVGAQGQTFRISVPLSVDG
                     ADQAAVLKTLNGVAAQIASRRSHQCFSVKPRFKAGGGDAHQLTVVPQRAGQFVRAVPG
                     GHADPYAGERSLSIGARALEDPAAVRILAAFFLKGEALGYDASVAALAVDLLARAASE
                     AQVPDCAWTGTLEITDVYDRFHLTVTSTLSFSFTVSPSGRVRGTVQPSTRSATVSESD
                     VGSCSGSGITMFEVRLEGAADAAGLTLTFSSHGEEPEFTVSCSGGSITFSGDYIKAPF
                     TLTFDRTLPVGLAERPGAARTMRGTRSGSAYTVTVTITRA"
     gene            complement(1549..2184)
                     /locus_tag="VEO00_01890"
     CDS             complement(1549..2184)
                     /locus_tag="VEO00_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56787.1"
                     /translation="MNRPFRPIRRARAILWLATVASLGLAACATPPDPVSEAGAAVLR
                     TFDPENEPDATFAELNRLAAESPEGVALAAASLVRDPKRSTRYVAVYLLGLTASTPAD
                     IAALGAALEDRETFIRAIAGGALVGLGHKPAIPVLIDLLASEVTIPQSFPPLPIARLA
                     FDGLSFYTGVSFGWEEAGGSGERAHVRERWQRWWVENGERLRWNPDSERYE"
     gene            2397..4280
                     /gene="ftsH"
                     /locus_tag="VEO00_01895"
     CDS             2397..4280
                     /gene="ftsH"
                     /locus_tag="VEO00_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HYU56788.1"
                     /translation="MYALLLLVGLWLFVSLATRAAPPKKLSVTELREAAASGAVASAT
                     FFERDLKIRGDLKNDTKYETAYPSEYQDELVDELTEAGVPIAADSQQGSIWVNALVSW
                     LPILLIAGLVIFMFQNMQGGGSRVMSFGRAKAKMVSKDQPKTTFADVAGVDEAIEELQ
                     EVKEYLENPAKFQAMGAKIPKGVLLYGPPGTGKTLLARAVAGEAGVPFFSISGSDFVE
                     MFVGVGAARVRDLFEQAKQNAPAIVFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVE
                     MDGFDTRTGVILMAATNRPDILDPALLRPGRFDRQVVIDRPDLAGRKAILAVHARGKP
                     LDRTVDLDVIARRTPGFTGADLSNVMNEAALLAARRKKQGVGLAEMEEAIDRVVAGPE
                     RKTRLISDREKRTIAYHESGHAIVAHALPNSDPVHKITIISRGRALGYTMTLPMEDKF
                     LVTREEFIDELAMLLGGRVAEEMVIGDITTGSQNDLDRATKIARQMVTEYGMSDSLGP
                     LTLGQKQHEVFLGRDFASHPDYSDQIAFEIDSEIRRLIDEAHDEALDVLKANRKKLDE
                     IAAALIEKETLEKDELLAILEGIEKRAVRNGGQKKSAAGIAVARRSVRRNGPAGRRGS
                     LGT"
     gene            4277..4930
                     /gene="folE"
                     /locus_tag="VEO00_01900"
     CDS             4277..4930
                     /gene="folE"
                     /locus_tag="VEO00_01900"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HYU56789.1"
                     /translation="MTATSFDRDRIAAGVRQILEGLGEDLTREGIARTPERVAEMFEE
                     ILGGTFEDPASILKVRFDEGHDEMIMVRDIPVASICEHHLVPWIGKAHVAYIPNRDGR
                     ITGISKLARVVDTFAHRLQVQERMTMQVADAIEQALEPRGVLVVVEAEHLCMTMRGVR
                     KPGSQTVTSAVRGLFRKDPRTRAEAFNLIDAGRRGAAISGPVRGCSGRCQARTGRGN"
     gene            4935..>5699
                     /locus_tag="VEO00_01905"
     CDS             4935..>5699
                     /locus_tag="VEO00_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU56790.1"
                     /translation="MNDLRNPDRVLATVLFTDIVGSTELAAEMGDQRWRELLDTHHAR
                     VRTELEQFNGREVKTMGDGFLATFDSPARAVLCARAIAESMTAIGVRIRAGIHTGETE
                     IMGEDVGGIAVHIAARVAGRADPDEILVSSTVKDLVEGSHIAFTPKGSRALALKGIPG
                     RWRLYAVRWQPERAASAGLPRRSEHPLVGRAAEMAVGRAQVDAAAAGSLRVLCVEGEP
                     GIGKTRLLDETAAYASERGFTVAFVGADEEVAGPFFL"
BASE COUNT          895 a         2011 c         2020 g          773 t
ORIGIN      
        1 gcgtgccggt cgatgcggct agactggcgg cccacgtgtc cgagtacgag tcagacatcg
       61 agcgcatcct catcaccgag gcggagatcc aggcgcgcct cgcagagctc ggagcgcaga
      121 tcaccgaaga ctatgccggg cgcgatccgc tgctcgtcgg cgtgctgaag ggggccttca
      181 tgctgatggc cgacctgtcg cggcacatcc acctgccgct cgagttcgac ttcatggcgg
      241 tctcgagcta cggctcggcg acgcggacgt ccggggtggt gcggatcctg aaggacctcg
      301 accacgagat ccagggccgc gacgtgctgc tggtcgagga catcatcgac agcggcctga
      361 ccctgaacta cctgctgaag aacctccgca gccgcaagcc cgggacgctc gaggtctgcg
      421 cgctgctgca caagaccgag atgcagcgcg ttcccctgga catccgctac acgggcttcg
      481 agattccgcc cgagttcgtg atcggctatg gcctggacta cgcggagagg ttccgcaacc
      541 tgccctacgt cgccaccctg aagcgcgagg cctacgccgc cggctgaata ggggcccgcc
      601 ctcaggcccg cgtgatcgtg accgtgacgg tgtacgcgct gccgcttcgg gtgccgcgca
      661 tggttcgtgc ggcgcccggt cgctcggcca gcccgacggg cagggtccgg tcgaacgtca
      721 gcgtgaacgg tgccttgatg tagtccccgc tgaacgtgat cgaccccccg ctgcaggaca
      781 cggtgaactc cggttcctca ccgtgcgagg agaacgtcag cgtcagcccc gcggcgtcgg
      841 ccgcgccctc cagccggacc tcgaacatcg tgatcccgga cccggagcag gaccccacgt
      901 cggactccga gaccgtcgcc gatcgcgtcg acggctggac cgtcccccgg accctgccgg
      961 agggggagac cgtgaacgag aaggacagcg tggaggtgac cgtcaggtgg aaccggtcgt
     1021 agacgtcggt gatctccagg gtccccgtcc aggcgcagtc ggggacctgg gcctcgctcg
     1081 ccgctcgggc cagcaggtcg accgccagcg ccgcgaccga ggcgtcgtag ccgagcgcct
     1141 ctcccttcag gaagaaggcg gcgaggatcc ggacggccgc ggggtcctcg agggcccggg
     1201 ctccgatcga gagagagcgc tctccggcgt acgggtcggc atggccgccc gggacggccc
     1261 ggacgaactg gccggcgcgc tgcgggacga cggtcagttg gtgcgcgtcg ccgcccccgg
     1321 ccttgaaccg cggcttcacc gagaagcact ggtgggagcg ccgcgacgct atctgggcgg
     1381 ccacgccgtt cagcgtcttc aacaccgcgg cctggtcggc gccgtcgacc gacaggggga
     1441 ccgagatccg aaaggtttgt ccttgggcgc cgaccgccgt gtcggccgcg gcgtttccgc
     1501 ccgccgtcag ggccacgagc agggacagca ggatcgccgc gcgacggctc actcgtagcg
     1561 ctccgagtcg gggttccagc gcagccgctc accgttctcg acccaccagc gctgccagcg
     1621 ctcccggacg tgcgctcgct ctccgctccc gccggcctcc tcccagccga agctcactcc
     1681 cgtgtagaac gacaggccgt cgaacgccag ccgggcgatc gggagcggcg ggaaggactg
     1741 cgggatcgtc acctcgctgg cgagcaggtc gatcagcacc gggatggcgg gcttgtgccc
     1801 cagtccgacc agcgccccgc cggcgatcgc ccggatgaac gtctccctgt cctcgagcgc
     1861 ggcgcccagt gcggcgatgt ccgcgggggt cgacgccgtc agcccaagca ggtagacggc
     1921 cacgtagcgc gtcgagcgct tcgggtcgcg caccaggctc gccgcggcca gagcgacgcc
     1981 ctcgggcgac tccgccgcca gccggttcag ctcggcgaac gtcgcgtccg gctcgttctc
     2041 ggggtcgaag gtgcgcagca cggcggcgcc ggcctcgctc acaggatccg gcggtgtcgc
     2101 gcacgccgcc agcccgaggg acgcgaccgt cgcgagccat aggatggcgc gagcgcgccg
     2161 gatcggtcga aacgggcggt tcacgcggag cctattggcg cacgcggcac gcgcgacggt
     2221 caacctccga ccggaggccg cggaggctcg gagcggcgcg gtcggtgcta ccatcgccgg
     2281 ggcatggccg aacgggccgg ccccgtttct gttcgcagac accggaccaa ggagcctcga
     2341 tcaaccccac ggcacccggc gagtcacgac tccgcaaggt cctccgaggc cccctggtgt
     2401 acgcgctgct cctgctggtc ggcctgtggc tgttcgtctc gctggccacc cgcgccgccc
     2461 cgccgaagaa gctcagcgtg accgagctgc gggaggcggc ggcctccggc gcggtggcct
     2521 cggcgacgtt cttcgagcgc gacctgaaga tccgcggaga cctgaagaac gacacgaagt
     2581 acgagaccgc ctacccctcc gagtaccagg acgagctggt cgacgagctg accgaggccg
     2641 gcgtcccgat cgccgccgac tcacagcagg gctcgatctg ggtgaacgcg ctggtctcct
     2701 ggctgccgat cctgctcatc gccgggctcg tgatcttcat gttccagaac atgcaaggcg
     2761 gcggcagccg ggtgatgtcc ttcggccgcg ccaaggccaa gatggtctcg aaggaccagc
     2821 cgaagacgac gttcgccgac gtcgccggcg tggacgaggc catcgaggag ctccaggagg
     2881 tcaaggagta cctcgagaac ccggccaagt tccaggccat gggcgcgaag atcccgaagg
     2941 gcgtgctgct ctacgggccg ccggggaccg gcaagacgct cctcgcccgc gccgtggcgg
     3001 gtgaggccgg cgtcccgttc ttctccatct cgggctcgga tttcgtcgag atgttcgtcg
     3061 gcgtcggcgc cgcccgggtg cgcgacctgt tcgagcaggc gaagcagaac gccccggcga
     3121 tcgtgttcat cgacgagatc gacgccgtcg gccgccaccg cggcgcgggc ctggggggcg
     3181 gccacgacga gcgcgagcag accctgaacc agctgctcgt cgagatggac ggcttcgaca
     3241 cgcgcaccgg cgtgatcctg atggcggcga ccaaccgccc cgacatactc gacccggcgc
     3301 tgctgcgccc gggccgcttc gaccgccagg tggtgatcga ccggcccgac ctcgccgggc
     3361 gcaaggcgat cctcgcggtc cacgcgcgcg gcaagccgct cgaccgcacg gtggacctcg
     3421 acgtcatcgc ccggcgcacg ccgggcttca ccggcgccga cctgtccaac gtgatgaacg
     3481 aggcggcact gctggcggcg cgccggaaga agcaaggcgt cgggcttgcc gagatggagg
     3541 aggccatcga ccgcgtcgtg gccggcccgg agcgcaagac ccggctgatc tccgaccgcg
     3601 agaagcggac catcgcctac cacgagtccg gccacgccat cgtcgcgcac gcgctcccga
     3661 actccgaccc ggtgcacaag atcacgatca tctccagggg tcgcgcgctc gggtacacga
     3721 tgacgcttcc gatggaggac aagttcctgg tcacccggga ggagttcatc gacgagctgg
     3781 cgatgctgct cggcggacgg gtggccgagg agatggtgat cggcgacatc accaccggct
     3841 ctcagaacga cctcgaccgc gccaccaaga tcgcgcggca gatggtcacc gagtacggga
     3901 tgtccgacag cctcggcccg ctgacgctcg gccagaagca gcacgaggtg ttcctcggac
     3961 gcgacttcgc gtcgcacccc gactactccg accagatcgc cttcgagatc gattcggaga
     4021 tccggcggct gatcgacgaa gcgcatgacg aggcgctcga cgtgctgaag gcgaaccgca
     4081 agaagctcga cgagatcgcc gccgcgctga tcgagaagga gactctcgag aaggacgagc
     4141 tgctggcgat cctcgagggg atcgagaagc gcgcggtccg caacgggggt cagaagaaga
     4201 gcgcggccgg gatcgccgtg gcccgtcgca gcgtgcgccg gaacggtccg gccggccgtc
     4261 gcggctccct ggggacgtga cggcgacctc cttcgaccgc gaccggatcg ccgccggcgt
     4321 ccgtcagatc ctcgagggcc tgggcgagga cctcacccgc gagggcatcg cccggacgcc
     4381 cgagcgcgtc gcggagatgt tcgaggagat cctcgggggc accttcgagg atcccgcctc
     4441 gatcctgaag gtccgcttcg acgagggcca cgacgagatg atcatggtcc gcgacatccc
     4501 cgtggcctcg atctgtgagc accatctggt tccgtggatc gggaaggcgc acgtggccta
     4561 catcccgaac cgggacgggc ggatcaccgg catctcgaag ctcgcccggg tcgtcgacac
     4621 gttcgcgcac cgcctgcagg tccaggagcg gatgaccatg caggtggccg acgccatcga
     4681 gcaggccctc gagccgcgcg gcgtgctcgt ggtcgtcgag gccgagcacc tgtgcatgac
     4741 gatgcgcggc gtccgcaagc ccgggtcgca gaccgtgacc agtgccgtcc gcggcctctt
     4801 ccgcaaggat cctcgtaccc gggccgaggc cttcaacctg atcgacgccg gccgacgcgg
     4861 agcggcgatc agcggccccg tccgcggatg cagcggccgt tgccaggcac ggacaggccg
     4921 gggaaactga acccgtgaac gacctccgca accccgaccg ggtgctcgca accgtactgt
     4981 tcacggacat cgtcgggtcg accgagctgg cggcggagat gggcgaccag cggtggcgcg
     5041 agctgctgga cacccaccac gcgcgggtgc ggaccgagct cgagcagttc aacgggcgcg
     5101 aggtcaagac gatgggcgac gggttcctgg ccaccttcga cagccctgcg cgcgccgtcc
     5161 tctgcgcccg ggcgatcgcc gagtcgatga ccgcgatcgg ggtgcggatc cgcgctggca
     5221 tccacaccgg cgagaccgag atcatgggcg aggacgtcgg cggcatcgcg gtgcacatcg
     5281 cggcgcgggt ggccggcagg gccgaccccg acgagatcct ggtctcgagc acggtgaagg
     5341 acctcgtcga ggggtcccac atcgcgttca cgccgaaggg cagccgcgcc ctggccctga
     5401 agggcatccc cggtcgctgg cgcctgtacg ccgtccgctg gcaaccggaa cgcgccgcgt
     5461 ccgcgggtct gccgcgccgc agcgagcacc ccctggtcgg ccgggctgcc gagatggcgg
     5521 tcgggcgggc gcaggtggac gcggcggccg ccggctcgct gcgggtgctg tgcgtcgagg
     5581 gcgagcccgg gatcggcaag acccgactgc tggacgagac cgcggcctac gcctctgagc
     5641 gcgggttcac cgtcgcgttc gtcggcgccg acgaggaggt ggccgggccc ttcttcctg
//
LOCUS       DASPWO010000084         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_270570, whole genome shotgun sequence.
ACCESSION   DASPWO010000084 DASPWO010000000
VERSION     DASPWO010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270570"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VEO00_01910"
     CDS             <1..1205
                     /locus_tag="VEO00_01910"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU56791.1"
                     /translation="SEEIHTALDELDPYAGSGERGWIMSELGGTRLRLGDLGGAETAF
                     VRAHELGWPPQLGLARLGLARGDIVRAASVIGDALDNPNDLPSFETPPHTDLRRAPLL
                     AAFVEVAVETGDVDRAGRAGADLTEIAERYGSTALHAAAAAARGDVALARGDAAAARE
                     EFETSIRLWRSVNAPYEGAAVRMRLGRAHQAAGRTDQALLEYRAAGQIFERLGARLDL
                     RKAEEAENALTGDRRGSRETKVFMFTDIVQSTSLAEALGDDAWEHLLRWHNEMLARTV
                     GEHGGELVRSMGDGLFVTFDQPEQAVDAAIEIQHALERHRREHGFAPSVRIGLHRAEA
                     TRDGTDWAGVGVHAAARIGALADGGEILTSRETAEAAGGARQVSEPSAVSLKGFKEPV
                     EVVRIEWR"
     gene            1363..>2184
                     /locus_tag="VEO00_01915"
     CDS             1363..>2184
                     /locus_tag="VEO00_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYU56792.1"
                     /translation="MTYDRGGERRLGALHQGRVIDLPDAVGHPAFPPTMEALVARNGG
                     TTLDAAREAFEHDDVLEFAVDDARLLVPILPASLRDFLAFEDHVRRGAERRGTPVPDA
                     WYEIPVYYKGNHRTVIGPEEVLPWPPFTEKLDFELEVACILGKPGRDLSEDQAAARIF
                     GYTVMNDWSARDLQAREMSAWLGPAKSKDFATSLGPCVVTADEFDPSDCRLSARVDGE
                     VWAEGNLKEMRWSFPQMVAYVSQGEDVEAGDVYGSGTFGGGCGLDLGRFLHPGAEV"
BASE COUNT          313 a          780 c          816 g          275 t
ORIGIN      
        1 cgtcggagga gatccacacc gcgctcgacg agctcgaccc ctacgccggg tctggcgagc
       61 gaggctggat catgtcggag ctcggcggga cccggctgcg gctgggagat ctcggcggcg
      121 cggagaccgc gttcgtccgt gcccacgagc tcggctggcc gccgcagctg gggctggctc
      181 ggcttggcct ggcgcggggt gacatcgtcc gagcggcgtc ggtgatcggc gacgcacttg
      241 acaacccgaa cgacctcccc agcttcgaga caccgccgca cacggacctg cgccgggcgc
      301 cgctcctcgc ggccttcgtg gaggtcgcgg tcgagacggg ggacgtggac cgcgcgggcc
      361 gggccggggc ggacctcacc gagatcgccg agcgctacgg cagcacggcc ctccatgcgg
      421 cggcggcggc ggcccgcggg gacgttgctc tggcgcgcgg agacgcggcc gccgcccgcg
      481 aggagttcga aacctcgatc cggctgtggc gctcagtgaa cgctccgtac gagggcgccg
      541 cggtccggat gcgcctgggc cgggcccacc aggccgccgg gcgaaccgac caggccctcc
      601 tcgagtaccg ggcagcggga cagatcttcg aacggctcgg cgcaaggctc gatctccgca
      661 aggccgagga ggccgagaac gcgctcaccg gcgaccggcg cgggtcgcgc gagacgaagg
      721 tcttcatgtt caccgatatc gtccaatcca cgagcctggc ggaggccctc ggcgacgacg
      781 cctgggagca cctcctgcgc tggcacaacg agatgctcgc ccgcacggtc ggcgagcacg
      841 gcggcgagct cgtccgctcg atgggcgacg gactgttcgt gaccttcgac cagccggaac
      901 aggcggtcga cgcggccatc gagatccagc acgcgctgga gcggcaccgc cgggagcacg
      961 ggttcgcgcc cagcgtccgg atcggcctcc accgggcgga ggccacccgc gacggcaccg
     1021 actgggcggg cgtgggggtg cacgcggccg cgcggatcgg cgccctggcc gacggcggcg
     1081 agatcctgac cagccgggag accgccgagg ctgccggagg agcccggcag gtctcggagc
     1141 cgagcgccgt gtcgctgaag ggattcaagg aaccggtcga ggtcgttcgc atcgagtggc
     1201 ggtgaggttc cggggcgctg ccggcaggcc tcccgcgatc acacttccaa acgacaggca
     1261 acagcagtaa cagcttggcc ggaggtgctt gcacctcgcc cggcggcggg cgagcattcg
     1321 gctccctccc ccccacggac gaaggacgca aggatgcggc tggtgacgta cgaccgtggc
     1381 ggggagcgcc gcctgggggc gctgcaccag gggcgggtga tcgacctccc cgacgccgtc
     1441 gggcaccccg ccttcccccc gacgatggaa gccctggtcg cgcgcaacgg cggcaccacc
     1501 ctggacgccg cccgggaggc cttcgagcac gacgacgtcc tcgagttcgc ggtggacgac
     1561 gcccgcctgc tggtcccgat cctccctgcc tccctgcgcg acttcctggc gttcgaggac
     1621 cacgtgcggc gcggcgccga gcgccgtggg acgccggtcc cggacgcctg gtacgagatc
     1681 cccgtctact acaagggcaa ccaccggacc gtgatcgggc cggaggaggt ccttccctgg
     1741 ccgccgttca ccgagaagct cgacttcgag ctcgaggtgg cctgcatcct gggcaagccc
     1801 ggccgcgacc tgagcgagga ccaggcggcg gcgcggatct tcggctacac ggtgatgaac
     1861 gactggtcgg cgcgcgacct gcaggcccgg gagatgagcg cctggctcgg cccggcgaag
     1921 tcgaaggact tcgccacctc cctcggcccg tgcgtcgtca ccgccgacga gttcgacccc
     1981 tccgactgcc ggctctcggc gcgcgtcgac ggcgaggtct gggcggaggg gaacctgaag
     2041 gagatgcgct ggagcttccc gcagatggtc gcctacgtct cgcagggcga ggacgtcgag
     2101 gcgggggacg tctacggctc cggcacgttc ggcggcggct gcggcctcga cctggggcgc
     2161 ttcctccacc ccggcgcgga ggtc
//
LOCUS       DASPWO010000085         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_525628, whole genome shotgun sequence.
ACCESSION   DASPWO010000085 DASPWO010000000
VERSION     DASPWO010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525628"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VEO00_01920"
     CDS             <1..552
                     /locus_tag="VEO00_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56793.1"
                     /translation="ENFGTPSQVSLGTGQMLNVVLPVGTHQITLRVTDHLDHTATDSL
                     VATTVDTTAPVIVSPLAVSPAVLSPPNHRLVTVAVPTITAHDDCDAAPRIFCSVGSSE
                     PPDARGDGSSAFDIVFDGQPVFTQSTGDRQIQTVNGVGTFSLQLRAERRGGQGGRTYT
                     IACHAVDANGNSGPPRVATVSVR"
     gene            660..1469
                     /locus_tag="VEO00_01925"
     CDS             660..1469
                     /locus_tag="VEO00_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYU56794.1"
                     /translation="MRPERLRDPRTGRVRTLGARQRGALLLRRLGLAGPAARKFRFVE
                     GDVEEPRLGLAPAEVRALEGALTHVVHCAASVAFDDSYENSFRANVLGSRNALDFSLR
                     LQHARGSPFVAHLATETSYVHGRRQGGVAPEARLVFPPRYYNNFYELTKARRPGLRKD
                     QGPRRDRPARMAVGGRDRRHGVRRRPLRRLVLRLGVPPVDGGAPRPGHVRAARESIGL
                     GVRLALGRSIVSRNHRVVVPVAAAAPGGTGPRGRMISARRSARAGSRSSRG"
     gene            complement(1355..>2665)
                     /locus_tag="VEO00_01930"
     CDS             complement(1355..>2665)
                     /locus_tag="VEO00_01930"
                     /inference="COORDINATES: protein motif:HMM:NF017142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYU56795.1"
                     /translation="AHFRSFPLTTDAPVHQVHAAADSEAALEALPPVVAGWKSLVAET
                     GALFGAPHYRSYSFLLTLSDHTAYFGLEHHESSDDRVRERSLVDEDARRVAAGLLPHE
                     MTHSWNGKYRRPTGLATGNFDTPMRGELLWVYEGLTTYLGQVLAARSGLLTADEYRES
                     LALTAAGMDHQRGRTWRPLLDTTIAAQILYGARGDRGAWRRGVDFYPEGELLWLEADA
                     LIRRESGGAKSLDDFCRLFYGGTSGPPQMVPYDFDDVVAALQKVAPHDWRGFWRSRVE
                     ETTTGAPLGGVTGGGWTLVYTDTVPQMLRSQEAVQKTVDLRFSIGITLAENGHIDDVI
                     PGSPADRAGVAPGMKLVAVNGRRLSPEILRQAIRETRSRARPLELLTENGEFFATHRL
                     EYDGGERYPRLERDPARADLLAEIIRPRGPVPPGAAAATGTTTR"
BASE COUNT          357 a          969 c          957 g          382 t
ORIGIN      
        1 gagaacttcg gcactccctc gcaggtgagc ctggggaccg gtcagatgct caacgtggtc
       61 ctgcccgtgg gcacgcacca gatcaccctg cgcgtgaccg accacctcga ccacaccgcc
      121 accgacagcc tggttgcgac caccgtcgac accacggctc ccgtcatcgt cagccccctc
      181 gcggtgtcac cggccgtgct cagcccgccg aaccaccggc tcgtcaccgt cgccgtcccc
      241 accatcacgg cccatgacga ctgcgacgcg gccccgcgca tcttctgctc ggtgggcagc
      301 agcgagccgc cggacgcccg aggcgacggg tccagcgcgt tcgacatcgt cttcgatggc
      361 cagccggtct tcacccagag caccggtgac cggcagatcc aaaccgtgaa cggggtgggc
      421 accttcagcc tgcagctccg tgcggagcgc aggggcgggc agggcggtcg cacctacacg
      481 atcgcctgcc acgcggtgga cgccaacggc aattcgggtc cgccgagggt cgccaccgtc
      541 agcgtgcgct gaggtctggt cacatccgga tcccgtgggt gcccgggttc ccgggcaccc
      601 acgggtcgct ttcccgcgcc ggtcgcgaga aagggcggtc gccggttggg tgctgcgtcg
      661 tgcggcccga gcggctgcgc gacccgcgga cggggcgcgt ccggacgctg ggcgcacgcc
      721 agcgcggcgc gctcctgctc cgccgcctcg gcctcgcggg ccccgccgcc cggaagttcc
      781 gcttcgtcga gggcgacgtg gaggagccga ggctgggcct cgcccccgcc gaggtccggg
      841 ccctcgaggg ggccctcacc cacgtggtcc actgcgcggc cagcgttgcc ttcgacgaca
      901 gctacgagaa ctcgttccgg gcgaacgtgc tcggctcgcg caacgcgctc gacttctcgc
      961 tgcgcctcca gcacgcccgg ggctcgccgt tcgtcgccca cctggcgacc gagacctcgt
     1021 acgtgcacgg ccggcggcag gggggcgtgg cgccggaggc gaggctcgtg ttcccgccgc
     1081 gctactacaa caacttctac gagctgacca aggcgagacg tccaggccta cggaaggatc
     1141 aaggaccccg acgagatcgc ccggcgcgaa tggctgtggg aggacgcgat cgacgccatg
     1201 gagttcggcg ccggccgctc cgtcgcctcg ttctccgcct cggagttccg ccagttgatg
     1261 gaggcgcgcc tcgacctggg cacgttcgag ccgcgcggga gtcgatcggg ctgggggtcc
     1321 gcctcgctct cggcagaagc atcgtgagcc gcaatcaccg cgtcgtcgtg ccggtcgccg
     1381 ccgccgcgcc cggggggacg ggtccccgcg gccggatgat ctcggccagg aggtccgccc
     1441 gggccgggtc gcgctcgagc cgggggtagc gctcgccgcc gtcgtactcc agccggtggg
     1501 tagcgaaaaa ctcgccgttc tcggtcaaca gctccagcgg ccgagcgcga gacctcgtct
     1561 cgcggatggc ctgccgcagg atctccgggg acaggcgacg gccgttcacc gccacgagct
     1621 tcatgcccgg cgcgaccccc gcgcggtcgg ccggcgagcc cgggatcacg tcgtcgatgt
     1681 ggccgttttc cgcgagggtg atcccgatcg agaagcgcag gtcgacggtc ttctgcacgg
     1741 cctcctgtga ccggagcatc tggggcaccg tgtcggtgta gacgagcgtc cagcccccgc
     1801 cggtgacgcc cccgaggggg gcgccggtcg tggtctcctc tacgcgtgag cgccagaagc
     1861 cgcgccagtc gtggggggcg accttctgca gggcggccac cacgtcgtcg aagtcatacg
     1921 gcaccatctg gggcgggccg ctcgtccccc cgtagaagag acggcagaag tcgtccaggc
     1981 tcttcgcgcc tccgctttcc cttcggatga gggcgtcggc ttcgagccag agcagctcgc
     2041 cctccgggta gaagtcgacc ccgcgccgcc aggcgccccg gtcgccccgc gcgccgtaca
     2101 ggatctgggc ggcgatcgtc gtgtcgagga gcggccgcca ggtgcggccg cgctggtggt
     2161 ccatgccggc ggcggtcagg gcgaggctct cgcggtactc gtccgccgtg agcagcccgc
     2221 tgcgcgcggc caggacctgt cccaggtagg tcgtgaggcc ctcgtatacc cagagcagct
     2281 cgccccgcat gggagtgtcg aagttcccgg tggcgaggcc ggtcggacgg cggtacttgc
     2341 cgttccagga atgcgtcatc tcgtggggga ggaggcccgc cgcgacccgg cgcgcatcct
     2401 cgtccaccag cgaccgctcc cggacccggt cgtcgctcga ctcgtggtgc tcgaggccga
     2461 agtatgcggt atggtcggag agggtgagca ggaagctgta ggaccggtag tggggcgccc
     2521 cgaagagggc gcccgtctcc gccaccaggc tcttccatcc ggccacgacc gggggcagcg
     2581 cttccagggc cgcctcgctg tccgcggcgg cgtggacctg atgcaccggg gcgtcggtgg
     2641 tcagcgggaa ggagcggaag tgggc
//
LOCUS       DASPWO010000086         4279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1036619, whole genome shotgun sequence.
ACCESSION   DASPWO010000086 DASPWO010000000
VERSION     DASPWO010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036619"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /gene="carB"
                     /locus_tag="VEO00_01935"
     CDS             <1..1833
                     /gene="carB"
                     /locus_tag="VEO00_01935"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HYU56796.1"
                     /translation="RLSALAAEDLREAKRFGFSDRRLAVLLGTGEGEVRAARRAAGVR
                     PVFKTVDTCAGEFAARTPYHYSTYEEESEVRPGERPRVVILGAGPNRIGQGIEFDYAC
                     VHAAFALREAGYEAVMVNSNPETVSTDYDTSDRLYFEPLTVEDVLAVCDAEDPVGVIV
                     QLGGQTPLSLAKALAAEGLRVLGTGPEAIDLAEDRGKFGALLRRLGIPAPPHGEATSA
                     EEAWAVAERVGYPVMVRPSYVLGGRAMEIVYTEEELAGYVRAATEASADHPVLIDRFL
                     EGAIEIDVDAVGDGERVFIGAVMEHIEEAGVHSGDSSCVIPPMTLSEEELDEIEEATR
                     RIAGAIGVRGLVNVQFALRDERAWVLEANPRASRTVPFVSKATGVPLAKVAARVVAGE
                     GLGVLEAAGVIPADPSWYRHLAHVAVKAAVLPFGRFPGVDTVLGPEMRSTGEVMGIAD
                     DLGTALAKAQAATGAGLPDAGTVFVSVSNRDKRAILFPAKRLADLGFSILATRGTAAV
                     LARSGVAVTPVAKVAEGHPNAADLILSGQVDLVINTPFGRGPRGDGYFIRTAAVRAGV
                     PCVTTIPGVLAAVQGIEAGRRRPAPPRALQDYLAGAAVAQEVAG"
     gene            1830..2726
                     /locus_tag="VEO00_01940"
     CDS             1830..2726
                     /locus_tag="VEO00_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HYU56797.1"
                     /translation="MIRAQCEVLSTRKAGTYWSVTLVAPEIADQAAPGQFVEVGMPSG
                     RSFLLRRPFSIHQASRRGGWAGTLELVWDVHGPGTEWLTTVKPHDLLDVIGPLGRPFP
                     YPTGTISNCLLVGGGYGAAPLYFLAEELRARGKRVDMIVGARTQDRVFKPVEGKRLSV
                     AITITTEDGSSGSRGRVSDVLPAMVEKYGAQAVYACGPNAMLRAVSEYCREHRIPCLV
                     AVEELMACGVGVCWTCVVPVIRPDGKGYENQRGCREGPVFNGARILWDRWMGEGPLRL
                     APTPPGGFEVPEEVRERWQTRT"
     gene            2738..3697
                     /locus_tag="VEO00_01945"
     CDS             2738..3697
                     /locus_tag="VEO00_01945"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HYU56798.1"
                     /translation="MPRVSLGVDLGAGVKLATPVVAASGCFGSGQEMAELIDLRRVGA
                     VVTKSVTLKAVKGMPTPRMAETPSGMLNAIGLQNPGVAAFIRKEGPWLEKAGVPVIVS
                     VAGKSVEEFVRVAVRVVELPGVVAIEANISCPNVERRNQVFACYPEQAAEVIGSVSRI
                     SRLPVFAKLTPDVTSIVEVADACVKAGAHGLTLINTLLGMAVDVETQRPRLGGVTGGL
                     SGPAIRPVAVRTVFQVSRALPAVPIIGVGGVAGAGDAAEMILAGAWAVGVGTANFYNP
                     SAPIEVAEGLGRILQEKGLRSPAELRGKVVGVVPVPESAHRGG"
     gene            3660..>4279
                     /gene="pyrF"
                     /locus_tag="VEO00_01950"
     CDS             3660..>4279
                     /gene="pyrF"
                     /locus_tag="VEO00_01950"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HYU56799.1"
                     /translation="MSRFPNPLIAAVDTSDPERAEALARGVAGAVAMLKVGLELFTAA
                     GPAAVRLLRRHAPVFLDLKLHDIPNTVRRAARNAGTLGAELLTVHALGGPAMVAAAVS
                     GVAEGAAAVGLAAPRVIAVTVLSSLAGEALASPASLAFEAVEAGAAGVVASGPDVALV
                     REALGPEPLLVVPGIRLVGGNGSGGDDQVRVLTPAEAIEAGADYLVV"
BASE COUNT          514 a         1501 c         1665 g          599 t
ORIGIN      
        1 cgcctctccg cgctcgccgc cgaggacctg cgcgaggcga agcgcttcgg gttctccgac
       61 cggcgcttgg cggtcctgct cgggaccggc gagggcgagg tccgcgccgc ccgccgcgcg
      121 gcgggcgtcc gtcccgtgtt caagaccgtc gacacctgcg ccggcgagtt cgcggcgcgc
      181 acgccctacc attactcgac ctacgaggag gagagcgagg tccgcccggg tgagaggccc
      241 cgggtggtga tcctgggggc cggcccgaac cggatcggcc agggcatcga gttcgactac
      301 gcctgcgtcc acgccgcctt cgcgctgcgg gaggccggct acgaggcggt gatggtcaac
      361 tcgaaccccg agaccgtctc caccgactac gacaccagcg accggctcta cttcgagccc
      421 ctgaccgtcg aggacgtcct ggccgtgtgc gacgccgagg atccggtcgg cgtgatcgtc
      481 cagctcggcg gccagacgcc gctgtcgctg gcgaaggcgc tggcggccga gggcctccgc
      541 gtgctgggga ccgggcccga ggcgatcgac ctggccgagg accgcggcaa gttcggggcc
      601 ctgctgcggc gcctggggat cccggcgccg ccccacggcg aggcgacgtc ggcggaggag
      661 gcctgggcgg tggccgaacg cgtcggctac ccggtgatgg ttcggccgtc ctacgtgctc
      721 ggcggccgag cgatggagat cgtctacacc gaggaggagc tggccggcta cgtccgggcc
      781 gccaccgagg cctccgccga tcaccccgtg ctgatcgatc ggttcctgga gggggccatc
      841 gagatcgacg tcgacgccgt cggcgacggc gagcgggtgt tcatcggcgc ggtgatggag
      901 cacatcgagg aggccggcgt gcactccggt gactcctcgt gcgtgatccc gccgatgacg
      961 ctctcggagg aggagctcga cgagatcgag gaggccaccc ggcggatcgc cggggcgatc
     1021 ggggtccgcg ggctggtgaa cgttcagttc gccctgcgcg acgagcgggc ctgggtgctc
     1081 gaggcgaacc cgcgggcctc gcgtaccgtg ccgttcgtgt cgaaggccac cggcgttccg
     1141 ctggcgaagg tggcggcgcg ggtcgtggcc ggcgagggcc tcggcgtgct ggaggcggcg
     1201 ggcgtgatcc cggcggaccc gagctggtac cgccaccttg cccacgtggc ggtgaaggcc
     1261 gcggtcctgc cgttcgggcg gttcccggga gtggatacag tcttgggacc ggagatgcgc
     1321 agcacgggag aggtgatggg gatcgccgac gacctgggca ccgcgctggc caaggcccag
     1381 gccgccaccg gtgccgggct gcccgatgcc ggcacggtct tcgtctcggt ctccaaccgc
     1441 gacaagcgcg cgatcctgtt cccggcgaag cgcctggccg acctgggctt ctcgatcctc
     1501 gccacccgcg gcacggccgc cgtgctggcg cgatccgggg tggccgtcac cccggtggcg
     1561 aaggtcgccg aggggcaccc caacgccgcc gacctgatcc tgtcggggca ggtcgacctg
     1621 gtgatcaaca cgcccttcgg gcgcggaccg cgcggggacg gctacttcat ccgcaccgct
     1681 gccgtccggg ccggcgtccc gtgcgtgacg acgatcccgg gggtgctggc ggccgtccag
     1741 gggatcgagg ccggccgccg ccgcccggcc ccgccccgcg cgctgcagga ctacctggcc
     1801 ggcgccgccg tcgcccagga ggtggccggg tgatccgcgc gcagtgcgag gtgctctcga
     1861 cgcgcaaggc cggcacgtac tggtcggtca cgctcgtcgc ccccgagatc gccgaccagg
     1921 ccgcgcccgg ccagttcgtc gaggtcggga tgccctccgg gcgctcgttc ctgctccggc
     1981 ggccgttctc catccaccag gcctcccgcc ggggcggctg ggccgggacc ctcgagctgg
     2041 tctgggacgt tcacgggccg gggaccgagt ggctgaccac cgtcaagccc cacgacctgc
     2101 tcgacgtgat cggcccgctc ggccggccgt tcccgtaccc gacgggcacc atctcgaact
     2161 gtctgctggt cggcggcggc tacggggcgg cgccgctcta cttcctggcc gaggagctcc
     2221 gcgcccgcgg caagcgcgtc gacatgatcg tcggcgcgcg cacccaggac cgggtgttca
     2281 agccggtcga ggggaagcgg ctgtcggtgg cgatcacgat caccaccgag gacggctcct
     2341 ccggctcgcg gggccgggtg tccgacgtgc tcccggcgat ggtcgagaag tacggcgccc
     2401 aggccgtcta cgcctgcggg ccgaacgcga tgctccgggc ggtgtccgag tactgccgag
     2461 aacaccgcat cccgtgcctc gtcgcggtgg aggagctgat ggcctgcggc gtgggcgtgt
     2521 gctggacctg cgtggtgccg gtgatccggc ccgacggcaa ggggtacgag aaccaacgcg
     2581 gctgccgcga gggcccggtg ttcaacggcg cccgcatcct gtgggaccgc tggatgggcg
     2641 aggggccgct gcggctggcg ccgaccccgc ctggcgggtt cgaggtgccg gaggaggtgc
     2701 gggagcgatg gcagacgcga acgtgagggt tcgcacgatg ccgcgggtct ccctgggggt
     2761 ggacctcggc gccggggtca agctggccac gccggtcgtg gccgcatcgg gctgcttcgg
     2821 gtccggccag gagatggccg agctgatcga cctgcgccgg gtcggggcgg tggtcacgaa
     2881 gagcgtgacg ctgaaggcgg tgaaggggat gccgacgccc cggatggccg agacgccgtc
     2941 ggggatgctg aacgcgatcg gcctgcagaa cccgggggtg gcggcattca tccgtaagga
     3001 gggcccctgg ctggagaagg ccggcgtgcc ggtgatcgtc tccgtggccg gcaagagcgt
     3061 cgaggagttc gtccgcgtgg ccgtgcgggt ggtcgagctc ccgggcgtcg tggccatcga
     3121 ggccaacatc tcctgcccga acgtcgagcg gcggaaccag gtgttcgcct gctacccgga
     3181 gcaggctgcc gaggtgatcg gctcggtctc ccggatctcc cggctcccgg tcttcgccaa
     3241 gctgaccccc gacgtcacca gcatcgtgga ggtggccgac gcgtgcgtga aggccggggc
     3301 gcacggcctg acgctgatca acacgctgct cgggatggcc gtcgacgtgg agacccagcg
     3361 tccccggctg gggggcgtca ccggcgggct gtccgggccc gccatccgcc cggttgccgt
     3421 gcggaccgtg ttccaggtct cccgggccct gccggcggtg ccgatcatcg gcgtcggggg
     3481 cgtggccggc gccggggacg ccgccgagat gatcctggcc ggcgcctggg ctgtcggcgt
     3541 cggcaccgcg aacttctaca acccgagcgc cccgatcgag gtggccgagg gcctcgggag
     3601 gatccttcag gagaagggcc tgcgctcgcc ggccgagctg cgcgggaagg tggtgggcgt
     3661 tgtcccggtt cccgaatccg ctcatcgcgg cggttgacac ctcggacccg gagcgagccg
     3721 aggcgctcgc ccggggggtg gccggggccg tggccatgct caaggtcggg ctggagctgt
     3781 tcaccgccgc cggcccggcc gccgtccgcc tcctccgccg gcacgccccc gtcttcctcg
     3841 acctgaagtt gcacgacatc ccgaacacgg tccgccgggc cgcgcgcaac gccgggaccc
     3901 tcggcgccga gctgttgacc gtccacgccc tcgggggacc ggccatggtg gcggcggccg
     3961 tgtccggcgt cgccgagggg gccgcggccg tcgggcttgc cgcgccgcgg gtgatcgccg
     4021 tgaccgtgct ctccagcctg gcaggggaag cgctggcgtc gccggcctcc ctggcgttcg
     4081 aggccgtgga ggctggcgcg gcgggcgtgg tggcctccgg ccccgacgtg gcgctcgtcc
     4141 gcgaggcgct cggcccggag ccgctcctgg tggtcccggg gatccggctg gtgggcggaa
     4201 acggcagcgg tggcgatgac caggtccgcg tgctcacccc cgccgaggcg atcgaggccg
     4261 gcgccgacta cctggtggt
//
LOCUS       DASPWO010000087         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_607503, whole genome shotgun sequence.
ACCESSION   DASPWO010000087 DASPWO010000000
VERSION     DASPWO010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607503"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..78)
                     /locus_tag="VEO00_01955"
     CDS             complement(<1..78)
                     /locus_tag="VEO00_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HYU56800.1"
                     /translation="MTWLVVGLGNPGERYAKTRHNLGRRV"
     gene            complement(100..807)
                     /locus_tag="VEO00_01960"
     CDS             complement(100..807)
                     /locus_tag="VEO00_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HYU56801.1"
                     /translation="MEKKLETKPREGHGKGVARKIRARGAVPAVMYGRDTTPVHIEVD
                     ARDLFHVLHTEAGMNVLIDLRVNGERHLTLARDVQRDIVRGQFLHADFLKIAHDEKIT
                     VDVPIALTGESAGVKEGGVVEHHLWELRVECLPTDVPQSIEADISALAIGDALKVEDL
                     KLPVSVTVLTPLEESVVAVVPPPILKLEEEEVAEAVEGEEAAEGAEGEGAPAAEGEGA
                     PAAEGGGSAEGESGGEG"
     gene            910..1473
                     /locus_tag="VEO00_01965"
     CDS             910..1473
                     /locus_tag="VEO00_01965"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HYU56802.1"
                     /translation="MAGVPRTSIETERLRLEPMSGDHLDGLWAAARSSLPELRAWMAW
                     SLETSPERMGAFVRDSEEAWAAGRAYRFAIVHDGEPVGVISVELHERMSHRGEVGYWM
                     RSDLAGRGLTTEAGHAVVGFAFGQLRLHRLDLSAGTGNPASQRVAEKLGFRREGLARN
                     GSSGEGGFHDVYTYGLLETDPRGPGRR"
     gene            1470..>2724
                     /locus_tag="VEO00_01970"
     CDS             1470..>2724
                     /locus_tag="VEO00_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYU56803.1"
                     /translation="MSGPVDPLAPFGRVTGRASLTVLRQGGEESASFDELLERGRRGA
                     TRLAARGVGPGDSVAILGEPSVAFHVGVLSAWAAGAACLPLPPPAPLELPASWAERTG
                     RVLATAGARVVVAPSGGAPADVPVPVVAAEDLDEGPGATPATPDDGDPGLILLTSGSS
                     SEPKGVVISFRAVAARAESQLLEGLDDDRDRFLTWLPPAWSGGFNAQLLRPLASGVSS
                     VVLPTGAVLEHPLRWLAEIGRWRATMSGCGTYGYGLVAGRLGHEPAPDVDLSCWTHVW
                     SAGERIDPDTIGRFLALAAPLGLDPRSFQPRYSLSECSTVASRPAGAGLAVDAVDAGG
                     LADGDARPSTGADAARFVSNGPPLRGVELWIEGPDGASLPERGVGEVRVRCPMMLDAY
                     LGDPERTAQALAGGWLRTGDLGYVAD"
BASE COUNT          296 a          995 c         1045 g          388 t
ORIGIN      
        1 cacgcggcgg ccgaggttgt ggcgcgtctt cgcgtagcgc tcgccggggt tccccagccc
       61 gaccacgagc caggtcatga gcggaggtcg agtggcggcc tagccctcgc cgccgctctc
      121 gccctcggcg gagccaccgc cctcggcggc cggcgcgccc tcgccctcgg cggccggcgc
      181 accctcgccc tcggcgccct cggcggcctc ctcgccctcc acggcctccg cgacctcctc
      241 ctcctcgagc ttcaggatcg gcggagggac cacggccacg acggactcct cgaggggggt
      301 gaggaccgtg acgctcaccg ggagcttcag gtcctcgacc ttcagcgcgt cgccgatggc
      361 gagcgcggag atgtcggcct cgatcgactg gggcacgtcg gtcgggaggc actccaccct
      421 cagctcccag aggtggtgct cgaccacgcc accctccttg acgcccgccg actcgccggt
      481 cagcgcgatc ggcacgtcaa cggtgatctt ctcgtcgtgc gcgatcttca agaagtcggc
      541 gtgcaggaac tggccgcgca cgatgtcgcg ctggacgtcg cgggccaggg tgaggtggcg
      601 ctccccgttc acgcgcaggt cgatcagcac gttcatcccc gcctccgtgt gcaggacgtg
      661 gaagagatcg cgcgcgtcga cctcgatgtg caccggcgtg gtgtcgcgcc cgtacatcac
      721 cgcggggacc gcgccgcgcg cgcggatctt gcgggcgacg cccttgccgt gcccttcgcg
      781 cggcttggtc tcgagcttct tctccatggg agtcggcgcc tttccatggc cggacgtgcg
      841 cacggccaac ggagcgatcg tagcagacgt ccccggcccc caccccggcc gagccgctac
      901 attcggccga tggccggcgt tccccgcacc tcgatcgaga ccgagcgcct gcgactggag
      961 ccgatgtccg gcgaccacct cgacggcctg tgggcggccg cccgatcgtc cctgcccgag
     1021 ctgcgcgcct ggatggcctg gtccctcgag acgagccccg agcgcatggg ggcgttcgtg
     1081 cgggactcgg aagaggcgtg ggcggccggc cgcgcctacc ggttcgccat cgtccacgac
     1141 ggcgagccgg tcggcgtgat cagcgtggag ctgcacgagc ggatgagcca tcgcggggag
     1201 gtcggctact ggatgcgcag cgacctcgcg ggccgagggc tgaccaccga ggcggggcac
     1261 gcggtggtcg gtttcgcgtt cgggcagctg cgcctgcacc ggctggacct ctccgccggc
     1321 accgggaacc cggccagcca gcgcgtcgcg gagaagctcg ggttccggcg cgagggcctg
     1381 gcccggaacg gatcttccgg tgagggcgga ttccacgacg tctacaccta cgggctgctc
     1441 gagaccgatc cgcggggccc gggccggcga tgagcgggcc ggtcgacccg ctggctccgt
     1501 tcgggcgcgt caccgggcgg gcctcgctca ccgtgctccg gcagggcggc gaggagtccg
     1561 cctcgttcga cgagctcctg gaacggggcc ggcgaggcgc gacgcgtctg gcggcccgcg
     1621 gcgtcgggcc gggcgactcg gtggcgatcc tcggcgagcc atcggtggcg ttccacgtgg
     1681 gggtgctgtc ggcctgggcg gccggcgcgg cctgcctccc gctcccgccg ccggcgccgc
     1741 tcgagctgcc ggcgtcgtgg gccgagcgga ccgggcgcgt gctcgccacc gccggcgccc
     1801 gggtcgtcgt ggcgccgtcg ggcggcgcgc cggccgacgt gccggtcccc gtcgtggccg
     1861 cggaggacct cgacgagggc ccgggcgcca cgccggcaac gccggacgac ggcgacccgg
     1921 ggctgatcct gctcacctcg ggaagctcgt ccgagccgaa gggcgtggtg atctcgttcc
     1981 gggcggtggc ggcgcgggcc gagagccagc tcctcgaggg gctcgatgac gaccgcgatc
     2041 gcttcctgac ctggctgccg cccgcgtgga gcggggggtt caacgcgcag ctgctgcgcc
     2101 cgctggcctc cggcgtgtcg tcggtcgtcc tgccgacggg ggcggtgttg gagcatccgc
     2161 tgcgctggct ggccgagatc ggccggtggc gggccacgat gtcgggctgc ggcacctacg
     2221 ggtacgggct ggtcgccggc cgactggggc acgagccggc gcccgacgtg gacctgtcgt
     2281 gctggacgca cgtgtggagc gccggcgagc gcatcgaccc cgacacgatc ggccggttcc
     2341 tcgccctggc agcgccgctc gggctcgatc cgcggtcgtt ccagccccgc tactcgctct
     2401 cggagtgctc gacggtggcc tcccgtcccg cgggtgcggg gctcgccgtg gacgcggttg
     2461 acgccggcgg gctggccgac ggcgacgcgc gaccgtcgac gggcgcggac gccgcccgct
     2521 tcgtctctaa cgggccgccc ctgcgcgggg tggagctgtg gatcgagggc cccgacggtg
     2581 cttcccttcc cgagcggggc gtcggggagg tgcgggtgcg ttgcccgatg atgctcgacg
     2641 cctatctcgg cgacccggag cggacggcgc aggcgctggc ggggggatgg ttgcgcaccg
     2701 gtgacctggg ctacgtggcg gacg
//
LOCUS       DASPWO010000088         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_863923, whole genome shotgun sequence.
ACCESSION   DASPWO010000088 DASPWO010000000
VERSION     DASPWO010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863923"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VEO00_01975"
     CDS             complement(<1..417)
                     /locus_tag="VEO00_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HYU56804.1"
                     /translation="MFKVSQKTEYAMRAMVELALRGDHGPVPAREIAESQRIPLRFLE
                     QQLAALAHAGLVKSHRGAGGGCELARDPAEITVAQIVDALEGPFYPMYCLDAADHTCF
                     QDARCGLQELWGDVHLAVRQVFEGTTVAGLARRHGRL"
     gene            592..1044
                     /locus_tag="VEO00_01980"
     CDS             592..1044
                     /locus_tag="VEO00_01980"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HYU56805.1"
                     /translation="MDTPRRMVDTRQPRFGQFLTGTVAAAAFVFRLPGLFVALAVILG
                     LAALSPATSPWRYLFLGFKRVAGLGPPKELEDAAPPRFANTLGFVFFTLASLLFYGLH
                     AEGPAYGIGALVAVLGFLGAAGFCVGCEIYLWIARARGRPVAATVQRG"
     gene            1049..1438
                     /locus_tag="VEO00_01985"
     CDS             1049..1438
                     /locus_tag="VEO00_01985"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYU56806.1"
                     /translation="MLIRALVLLAVIGAAGTGWWVLKRAPRGLTRLDLPELGVSGPAI
                     VQFSAPYCGPCTWTAPVLARTAAEASVTFRSVDVAACPELASRYHIRTVPTIVVAAAD
                     GSVLGTWTALPQNGEIAHAAQAAALAR"
     gene            complement(1495..1791)
                     /locus_tag="VEO00_01990"
     CDS             complement(1495..1791)
                     /locus_tag="VEO00_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HYU56807.1"
                     /translation="MEPVIMEVLAGARTDEREVDLCRLLLPFDLLAFEVVADFDGAAR
                     IYRRCRAAGVTPTRHGRLHDRRRRRRRDATLLAHDADLDRVARVVGIGLDAASL"
     gene            complement(1878..>2080)
                     /locus_tag="VEO00_01995"
     CDS             complement(1878..>2080)
                     /locus_tag="VEO00_01995"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449904.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HYU56808.1"
                     /translation="FAETAGGVTIGVLRKLREAGVVRADESVVAYVTGTGLKTIDALE
                     ESVAPTHTVSASLGEFQAQLGA"
BASE COUNT          282 a          779 c          716 g          303 t
ORIGIN      
        1 gagccggccg tggcgccggg ccaggccggc caccgtcgtc ccctcgaaca cctggcgcac
       61 cgcgaggtgc acgtcgcccc acaactcctg cagcccgcag cgcgcgtcct ggaagcacgt
      121 gtggtcggcc gcgtccaggc agtacatcgg gtagaagggc ccctcgagcg cgtccacgat
      181 ctgggccacc gtgatctcgg cggggtcgcg agcgagctcg catccaccgc cggcaccgcg
      241 atggctcttc accaggcccg cgtgggccag cgcggcgagc tgctgctcca ggaaccgcaa
      301 cgggatccgc tgggactccg cgatctcgcg ggccgggacg ggcccgtggt cgccgcggag
      361 cgccagctcc accatggccc gcatggcgta ctccgtcttc tgcgagacct tgaacatcac
      421 ggctccttcg ccggatacga ctgtacgacc gccgatatcg acggtcaacg gatcagccgt
      481 acgtgcccag ggcaacagcc cccgcaccat tcgacgtaat catacttcag cagtaggatt
      541 tgcaccgcat cgcggaagac ggcccgttcg atcgaggagg tatcgcccgg catggacacc
      601 ccgcggcgca tggtcgacac ccgacagcca cggttcggac agttcctgac tggcaccgtc
      661 gccgccgccg ccttcgtctt caggctgccc gggctgttcg tcgccctggc cgtgatcctc
      721 ggcctggcgg ccctctcccc ggccaccagc ccgtggcgct acctcttcct cggcttcaag
      781 cgcgtggccg gcctcggccc gccgaaggag ctggaagacg cggccccccc gcgcttcgcc
      841 aacacgctcg ggttcgtgtt cttcaccctg gcgagcctcc tgttctacgg cctgcacgcg
      901 gagggtcccg cctacgggat cggcgcactc gtcgcggtgc tcggcttcct cggcgccgcc
      961 ggcttctgcg tcggctgcga gatctacctg tggatcgcgc gggcccgcgg gcgcccggtg
     1021 gcggccacgg tccagcgggg atagcgcggt gctcatccgc gcgctcgtgc tgctcgccgt
     1081 cattggcgcc gccgggaccg gctggtgggt cctgaagcgg gcgccacggg ggctgacccg
     1141 gctcgacctg ccggagctcg gcgtgtccgg gccggcgatc gtgcagttct cggcgccgta
     1201 ctgcgggccc tgcacgtgga ccgcgccggt gctcgcgcgc accgcggccg aggcgagcgt
     1261 gacgttccgc tcggtggacg tggcggcgtg cccggagctc gcctcgcgct atcacatccg
     1321 aacggtgccg acgatcgtgg tcgccgcggc cgacggctcg gtgctcggca cctggaccgc
     1381 cttgccgcag aacggcgaga tcgcccacgc cgcacaggca gcggcccttg cgcgctgacc
     1441 gattcggccg gtagcgcccc gcgggcagga cttcgtccgg agccggcgtc ttcctcagag
     1501 tgacgcggcg tcgaggccga tccccacgac ccgggccacc cgatccaggt cggcgtcatg
     1561 ggcgagcaag gtcgcgtccc gacgccggcg acggcggcga tcatgcagtc gaccatgccg
     1621 cgtgggggtg acgccggccg cgcggcagcg gcggtagata cgggccgcgc cgtcgaagtc
     1681 cgccaccacc tcgaaggcca gaaggtcgaa cggcaggagc aaccggcaga ggtcgacctc
     1741 ccgctcgtcg gtccgggctc cggcgagcac ctccatgatc accggctcca tgaccgcaac
     1801 cggaccgtcg gcggcgatga gctcagtcag gcgccgatcg acgctccttg accgaaccgt
     1861 atgagatgtg gagccgatca ggcgccgagc tgggcctgga actcccccag gctggcgctg
     1921 accgtgtgcg tcggcgccac cgactcctcg agggcgtcga tcgtcttcag gccggtgccg
     1981 gtgacgtagg cgacgacgga ctcgtcggcg cgtacgactc cggcctcgcg aagcttgcgc
     2041 agcaccccga tcgtcacccc gccggcggtc tcggcgaaca
//
LOCUS       DASPWO010000089         8651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_271816, whole genome shotgun sequence.
ACCESSION   DASPWO010000089 DASPWO010000000
VERSION     DASPWO010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271816"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1659)
                     /locus_tag="VEO00_02000"
     CDS             complement(<1..1659)
                     /locus_tag="VEO00_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HYU56809.1"
                     /translation="MDHRPWFASYPPDVPHTLAPYPEQSVFTMLEDAVRRFPDRPALA
                     WFNNRMTYRQLLAEVERFSRVLTAQGLRKGDRLGIVLPNSPQFVIAWYACQRLGLVAV
                     GNNPLYTQREMTHQLKDAGIEVVVVLDSLYPLIGAIRDEVGLSTVIVTTIPEYMKFPL
                     KQLAPIKLKKDARAEGHAWPPVPADTDVKWWKDLMRAGGPEAPVAAVNATEDVAALVY
                     TGGTTGLSKGAMLTHHNLVTNALQTGSWFVDVKDGEEAIMCVLPFFHSYGLSAVMNLG
                     ISRGMKLVLMPRFELKMALKAIQKEKVTLFPGVPRIYIAINESPETRAFDISTIRACF
                     SGAAPLPTAVQEKFEGITGGRLVEGYGLTETSPVTHINPIYGKRKAGSIGLPIPDTDC
                     KIVDLDDPGKALAQGEEGELAIAGPQVMKGYWNRPEETADMIRADADGVRWLYTGDVA
                     RVDEDGYFSIVDRKKDMIIVSGFNVYPTDVEQVMYRHPKIQKVCVVGVPDDTTGEAVK
                     AFVVLKDGETATAEEILEWTRDEKHGLTGYRAPRLIEFRDELPET"
     gene            1889..4219
                     /locus_tag="VEO00_02005"
     CDS             1889..4219
                     /locus_tag="VEO00_02005"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HYU56810.1"
                     /translation="MATAARAALAPVEGELRLPGLREPAEVIRDRWGVPHIYAGSEAD
                     LFRAQGFIVASDRQLQLDVMCRIGNGRLAELFSELTLPLDRFIRTMGWNRVAARLTER
                     RGQAELDALIPFVEGQLAWAETAPAPAPEYVVLEAEPFVPASVEEALVWGAGAQVVMA
                     FTLSRNWDIELLRAAIAERLDAEAVQELFPDLPFEPGVVQGNRRAASPLDLLRAASPE
                     LTSNGGQGSNNWVVAGSRTASGKPLLANDPHLLVQLPSIWYEVHLVCPTMNVSGVALP
                     FAPGVVIGHNERIAWGFTNTEGDVQDLYLERLNDDGTAALYDGAWEPVTVHREELSVR
                     GRSAPEVLEVRETRHGPILDSYLVGIADTEVVTGGLHDSYAYRWVGAEHGISLEAVLG
                     IDRARTFDEFRLALAGWSCPGQNMVYADVDGNIGYQLTGLYPRRRRGDGSVPVPGWTS
                     DFEWDGFVPFEELPWSFNPEQGFIVTANNRMVDHAYPHVITRDWMPPHRARRIAERLT
                     ATPRHTAETFAAMQMDTVSLTAREVLPHLRALEPAGDRQKEALALLEEWDGDLGADSA
                     AAALYQVWTVRFAEHVLRPRLGEELYRHYFAKRHWAHTFRHQVLPELLTYPTARWFGA
                     EGPEARDRAMRAALEAAIEELTARLGEDPAAWRWGAIHEVRFSGRLAIIPDLADLFTA
                     GAVEMGGDDNTVCQALYEPQSGYGVAVIPSWRQVMDLGDPDASLGAHPPGQSGNPASP
                     HFADLLPLWAEGRGHPLPLSRPGVEEIAATTLTLAP"
     gene            complement(4247..4393)
                     /locus_tag="VEO00_02010"
     CDS             complement(4247..4393)
                     /locus_tag="VEO00_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56811.1"
                     /translation="MFRFLRRHQPKPTPLAVLYWAGLALVSLVVLFALFYLVDAYLPG
                     GGMF"
     gene            complement(4394..5125)
                     /locus_tag="VEO00_02015"
     CDS             complement(4394..5125)
                     /locus_tag="VEO00_02015"
                     /inference="COORDINATES: protein motif:HMM:NF033747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HYU56812.1"
                     /translation="MAVDATPLVGHRRVAARRRRWLNRLGKALITIGVLLLLFVAWEL
                     VGTNFLAARSQAALRAELEAEGLPGYDIVEDRDAHPRPNTARPFVKPGDGIGYLRVPK
                     IGVDKVFVEGIDRDALRKGPGHYEDTPLPGQKGNVGIAGHRTTYGAPFWALNRLRRGD
                     RIAVETARGRFVYRVIWVKVVAPDDVWVLNPTRVRLSITLTTCTPRFSASQRLVVRAV
                     QVIGPHPPGTRLASQRISDEPEDGA"
     gene            5170..5421
                     /locus_tag="VEO00_02020"
     CDS             5170..5421
                     /locus_tag="VEO00_02020"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HYU56813.1"
                     /translation="MPRSKSKRQRYQPPPRKKPKPSPKWFGGLILGLLLAGVVVILGY
                     YGLVPGFPWAGDQPGANPALLFIGLGLIAVGFGAATQWR"
     gene            complement(5516..6283)
                     /locus_tag="VEO00_02025"
     CDS             complement(5516..6283)
                     /locus_tag="VEO00_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYU56814.1"
                     /translation="MPLVTAPDGLRIAYEVAGEGGPPLVLHHGLGGDRRQWWSAGYVD
                     AFRTNTRVVLVDALGRGESDRPHDPAAYGIPRHAADVLRVLDAEGVERAVFWGWSVGG
                     RVGFEIAARHPDRLVALVATGATGHGFEPDPDELEEHLALFDRGMEDVVAVWSENTRA
                     PAWLRAQWLESDPAAIVASLRANAGYRGIADALPRLTVPVLMLIGDRDPLLPLARETA
                     ATAPRAVLAELEDRDHTTAFTESDVPVALTLEFLRGP"
     gene            6304..6549
                     /locus_tag="VEO00_02030"
     CDS             6304..6549
                     /locus_tag="VEO00_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYU56815.1"
                     /translation="MPVYEYQCRACGRRLEEVQAMGAAPPGPCPHCGGELRKVFSRVG
                     VRFRGWGFRSTDRLIPGDRPRKDFDTLQRKADEIAEG"
     gene            complement(6546..6818)
                     /locus_tag="VEO00_02035"
     CDS             complement(6546..6818)
                     /locus_tag="VEO00_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56816.1"
                     /translation="MPFALRVLIAFAIGGVILFVGYRFLRAIGAGAGQAHEEEPEDVG
                     DLDVFFVCAECGTEYKVTRLGELTVPRHCGEPMEVVRRPVQHPELN"
     gene            complement(6864..8039)
                     /locus_tag="VEO00_02040"
     CDS             complement(6864..8039)
                     /locus_tag="VEO00_02040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYU56817.1"
                     /translation="MPEAVIVEAVRSPMGKRGGKLSGWHPVDLLAEVVRSAVERSGID
                     PNLVEDHITGCVSQTGEQGLNIARNAWLAAGLPEEVPGTTIDRQCGSSQQAAHFAAQG
                     VIAGAYDVVIASGVEHMTRVPMGVTASQGPGFPFSPQLMDHYEGGLVPQGISAEIIAK
                     RWSLTRQELDEFAARSHTRAGAAIQAGKFHQEIQPVKVQTEDGGVELMDTDEGVRYPP
                     NRHKIAELAPAFKEDGVVTAANSSQITDGAAALVITSREKAEELGLRPRARFHAFALA
                     GVDPITMLTGPIPATQRVLAKAGLSLEDISVVEINEAFATVVLAWKKELGIPDDWFEE
                     NVNPNGGAIALGHPLGASGARLMTTLLHELERREARYGLQTMCEGGGMANATIIERL"
     gene            complement(8162..8590)
                     /locus_tag="VEO00_02045"
     CDS             complement(8162..8590)
                     /locus_tag="VEO00_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYU56818.1"
                     /translation="MDPDRLMALPLFAGLSQKDREAVARWADEVEVPEGKHLVDEGRF
                     AYEFFVIEKGSASVVHGGAEVATLGPGDFFGEIGLLQTERRTASVIAGTPMRLIVIFA
                     REFQRMAKEMPALADRVRAAMLERGRSLREADREADRPSD"
BASE COUNT         1190 a         3176 c         3011 g         1274 t
ORIGIN      
        1 cgtctcgggg agctcgtcgc ggaactcgat cagcctcggc gcccggtacc cggtcaggcc
       61 gtgcttctcg tcgcgggtcc actcgaggat ctcctcggcg gtggcggtct cgccgtcctt
      121 cagcacgacg aacgccttga ccgcctcccc ggtggtgtcg tcgggcacgc cgaccacgca
      181 gaccttctgg atcttcgggt ggcggtacat gacctgctcg acgtcggtcg ggtacacgtt
      241 gaagccggac acgatgatca tgtccttctt ccggtcgacg atcgagaagt agccgtcctc
      301 gtcgacgcga gcgacgtccc ccgtgtacag ccagcgaacc ccgtcggcgt cggcgcggat
      361 catgtcggcc gtctcctcgg gccggttcca gtaccccttc atcacctgcg gcccggcgat
      421 ggccagctcc ccctcctcgc cctgggcgag cgccttcccg ggatcgtcca ggtcgacgat
      481 cttgcagtcc gtgtcgggga tcggaagccc gatcgacccg gccttgcgct tgccgtagat
      541 cgggttgatg tgggtgaccg gtgacgtctc ggtcagtccg taaccctcga ccaggcgccc
      601 gccggtgatg ccctcgaact tctcctgcac cgccgtcggc agcggcgcgg cccccgagaa
      661 gcatgcccgg atcgtggaga tgtcgaaggc cctcgtctcc ggcgactcgt tgatcgcgat
      721 gtagatccgc ggcacgccgg ggaagagcgt caccttctcc ttctggatcg ccttcaacgc
      781 catcttcagc tcgaaccgcg gcatcagcac gagcttcatc ccgcgcgaga tcccgaggtt
      841 catcaccgca gaaagcccgt aggagtggaa gaacggcagg acgcacatga tcgcttcctc
      901 gccgtccttc acatccacga accacgaccc ggtctgcagg gcgttcgtca ccaggttgtg
      961 gtgggtgagc atcgcgccct tcgacagccc ggtggtcccg cccgtgtaga ccagcgcggc
     1021 cacgtcctcc gtggcgttca ccgccgcgac gggcgcctcc gggccgcccg ccctcatcag
     1081 atccttccac cacttcacgt cggtgtcggc ggggaccggc ggccacgcgt ggccctcggc
     1141 cctggcgtcc ttcttcagct tgatcggtgc gagctgcttc aacgggaact tcatgtactc
     1201 cgggatcgtg gtcacgatca ccgtggagag gcccacctcg tcgcggatcg cgccgatcag
     1261 ggggtagagc gagtcgagga cgacgacgac ctcgatgccg gcatccttca gctggtgggt
     1321 catctcccgc tgcgtgtaga gcgggttgtt ccccaccgcc accaggccga ggcgctggca
     1381 ggcgtaccac gcgatcacga actggggcga gttcgggagg acgatcccga ggcggtctcc
     1441 cttgcgcaac ccctgggccg tcaggacgcg cgagaaccgc tcgacctcgg cgagcagctg
     1501 ccggtacgtc atccggttgt tgaaccaggc cagggccgga cggtcgggga accggcgtac
     1561 cgcgtcctcg agcatggtga acaccgactg ctccgggtac ggcgcgaggg tgtgcgggac
     1621 gtccgggggg tagctggcga accagggccg gtgatccatc cgactctcct cctgggggcc
     1681 tcggggatgc aggtggatta tccccgcgcc ttcaccggcc gctcaaccgc cgttcgcccc
     1741 gccccgcacg ggacccggcg tttgccccgc cgacacagtc cctctacggt gccgcttcac
     1801 cgatgaccga gccagggacg actcgacggg tggggggtgg ggagggtctc ctccccacgt
     1861 cgacgagatg acgcaatggg ctgagagggt ggcgacggcc gcccgggccg cgctggcccc
     1921 ggtggagggc gagctgcggc tcccggggct gcgcgagccg gccgaggtga tccgcgaccg
     1981 ctggggcgtg ccgcacatct acgccggcag cgaggccgac ctcttccggg cgcaggggtt
     2041 catcgtcgcc tcggaccgcc agctgcagct cgacgtgatg tgccgcatcg ggaacggccg
     2101 gctggccgag ctgttctccg agctgacgct ccccctggac cggttcatcc gaacgatggg
     2161 ctggaaccgc gtcgccgccc ggctgaccga gcgccgcggc caggccgagc tcgacgcgct
     2221 catcccgttc gtcgagggcc agctggcctg ggccgagacc gcccccgcgc cggcccccga
     2281 gtacgtcgtg ctcgaagcgg agcccttcgt ccctgcgtcg gtggaggagg cgctcgtctg
     2341 gggcgcgggt gctcaggtgg tgatggcctt caccctgtca cggaactggg acatcgagct
     2401 gctgcgcgct gccatcgccg agcggctcga cgcggaggcc gtccaggagc tcttccccga
     2461 cctcccgttc gagcccggtg tggttcaggg caaccggcgc gccgcctccc cgctcgacct
     2521 gctccgcgcg gcgtcccccg agctgacgtc gaacggcggc cagggctcga acaactgggt
     2581 ggtggccggc agccggacgg cgagcgggaa gccgctcctg gcgaacgacc cacacctgct
     2641 cgtccagctg ccctcgatct ggtacgaagt tcacctggtc tgcccgacga tgaacgtctc
     2701 gggggtggcg ctgccgttcg cgccgggcgt ggtgatcggg cacaacgagc ggatcgcctg
     2761 gggcttcacg aacacggagg gtgacgtcca ggacctgtac ctcgagcggc tgaacgatga
     2821 cggcaccgcc gccctctacg acggcgcctg ggagcccgtg accgtccatc gcgaggagct
     2881 ctcggtgcgc ggccggtccg cgcccgaggt gctcgaggtg cgcgagaccc gccacgggcc
     2941 gatcctcgac tcctacctgg tcggcatcgc cgacaccgag gtggtcaccg gtggcctcca
     3001 cgactcctac gcgtaccgct gggtgggcgc cgagcacggc atctcgctgg aggcggtgct
     3061 ggggatcgac cgcgcccgga cgttcgacga gttccggctg gccctggccg ggtggtcctg
     3121 cccgggccag aacatggtct acgccgacgt cgacggcaac atcggctacc agctcaccgg
     3181 gctctaccca cgccgccgtc gcggggacgg ctcggtcccg gtccccggct ggacgtcgga
     3241 cttcgagtgg gacggattcg tcccgttcga ggagctcccc tggtccttca atccggagca
     3301 ggggttcatc gtcacggcga acaaccggat ggtcgaccac gcctatccgc acgtgatcac
     3361 ccgcgactgg atgccgcccc accgggcgcg gcggatcgcc gagcggctca ccgcgacccc
     3421 gcgccacacg gccgagacgt tcgcggcgat gcagatggac accgtgtcgc tgacggcgcg
     3481 cgaggtcctg ccccacctgc gggccctcga gcccgccggc gaccggcaga aggaggcgct
     3541 ggcgctgctc gaggaatggg acggcgacct gggcgccgac tccgccgccg cggccctcta
     3601 ccaggtgtgg acggtccgat tcgccgagca cgtcctgcgc ccgcgcctcg gcgaggagct
     3661 gtaccgccac tacttcgcca agcggcactg ggcgcacacg ttccgccacc aggtgctccc
     3721 ggagctcctg acctacccca ccgcccgctg gttcggggcc gaggggccgg aggctcgcga
     3781 ccgggcgatg cgcgcggcgc tcgaggcggc gatcgaggag ctgacggcgc ggctgggcga
     3841 ggacccggcg gcgtggcgct ggggcgcgat ccacgaggtg cggttctccg ggcgcctggc
     3901 gatcatcccc gacctggccg acctgttcac cgccggcgcg gtcgagatgg gcggcgacga
     3961 caacaccgtc tgccaggcgc tgtacgagcc ccagtcgggc tacggggtgg cggtcatccc
     4021 ctcgtggcgg caagtgatgg acctcggcga ccccgacgcc agcctcggcg cgcatccccc
     4081 gggccagtcc ggcaacccgg ccagcccgca cttcgccgac ctcctgccgc tgtgggccga
     4141 gggccgcggc cacccgctgc cgctgtcgcg tccgggcgtg gaggagatcg ccgcgaccac
     4201 cctgacgctg gctccgtgag cggcggctcc ctgagcggcg ccgcgctcag aacatcccgc
     4261 cgccgggaag gtacgcgtcg accagataga agagggcgaa caggaccacc aggctgacca
     4321 gcgcgagccc cgcccagtac agcacggcga gcggggtggg cttcggctga tggcgtcgca
     4381 ggaaccggaa cacctaggcg ccgtcctccg gctcgtcgga gatccgttgt gaggcgagcc
     4441 gggtccccgg cggatgcggc ccgatcacct gcacggcccg caccaccagg cgctgggagg
     4501 cggagaagcg cggggtgcag gtcgtcagcg tgatcgacag gcggacccgc gtcgggttca
     4561 gcacccacac gtcgtccggg gcaaccacct tgacccagat cacgcggtag acgaatcggc
     4621 cgcgggcggt ctccaccgcg atgcggtcgc cgcgccgcag ccggttgagc gcccagaacg
     4681 gcgcgccgta ggtcgtgcgg tgcccggcga tgccgacgtt gcccttctgt cccgggagcg
     4741 gggtgtcctc gtagtgcccg ggacccttgc gcagggcatc gcgatcgatc ccctcgacga
     4801 acaccttgtc gacgccgatc ttcgggacgc gcaggtagcc gatcccgtcg cccggcttca
     4861 cgaagggccg cgccgtgttc ggccgggggt gtgcgtcccg gtcctcgacg atgtcgtagc
     4921 ccggcaggcc ctccgcctcg agctcggctc gcagggcggc ctgcgaccgc gcggccagga
     4981 agttggtgcc caccagctcc caggccacga acagcaggag gaggacgccg atcgtgatca
     5041 gggccttccc cagccggttc agccagcggc ggcggcgggc ggcgacccgg cggtgaccga
     5101 ccaggggcgt ggcgtcgacg gccattcggg ttcggggcag tctagcgccc gcggtaggat
     5161 gcccgggcga tgcccagatc gaaatcgaag cggcagcgct accagccgcc cccgcgcaag
     5221 aagccgaagc cgtcgccgaa gtggttcggc gggctgatcc tggggctcct gctcgccggc
     5281 gtggtggtga tcctcgggta ctacggcctc gtgccggggt tcccgtgggc cggcgaccag
     5341 ccgggcgcga atccggccct gctcttcatc gggctggggc tcatcgcggt cggcttcggc
     5401 gcggccaccc agtggcgata gccccggtgg cccccatcgc tgtaactaca cgcctgtaac
     5461 cattccacac agttgtcccc acctgtggaa aacgccacgg cgggacgccg gagccctacg
     5521 ggccgcgcag gaactccagg gtcagcgcga ccgggacgtc gctctcggtg aacgcggtgg
     5581 tgtggtcacg gtcctcgagc tccgcgagca cggcccgagg ggccgtcgcg gcggtttcgc
     5641 gggcgagcgg caggagcggg tcccggtccc cgatcagcat caacaccggg accgtgagcc
     5701 ggggcaacgc gtccgcgatc ccccggtagc cggcgttcgc gcggagcgag gccacgatcg
     5761 cggcggggtc cgactcgagc cactgcgcgc gcagccacgc gggcgcgcgc gtgttctcgc
     5821 tccaaacggc cacgacgtcc tccatgcccc ggtcgaacag cgccaggtgc tcttccagct
     5881 cgtcggggtc gggctcgaac ccgtgcccgg tggcgccggt cgccaccagc gcgacgagcc
     5941 ggtcgggatg gcgcgcggcg atctcgaacc cgacgcgacc gcccaccgac cacccccaga
     6001 acaccgcccg ctcgacgccc tcggcgtcga ggacgcgcaa cacgtcggcg gcgtggcgcg
     6061 ggatgccgta cgccgcgggg tcgtgggggc ggtcgctctc gccgcgcccc agggcgtcca
     6121 ccagcaccac ccgcgtgttg gtgcggaacg cgtccacgta gcccgccgac caccactgcc
     6181 ggcggtcgcc gcccagcccg tgatggagca cgagcggcgg cccgccctcg ccggccacct
     6241 cgtaggcgat gcgcagcccg tccggggcgg tcaccaacgg catcgcgcga ggataatcgg
     6301 ggcgtgccgg tctacgagta ccagtgccgc gcctgcggcc gtcgcctgga ggaggtccag
     6361 gcgatgggcg ccgcgccgcc gggcccgtgc ccgcactgcg gtggcgagct gcgcaaggtg
     6421 ttctcccgcg tcggcgtccg cttccgcggc tggggcttcc gctccaccga caggctgatc
     6481 cccggcgatc gcccgcgcaa ggacttcgac accctccagc gcaaggccga cgagatcgcc
     6541 gagggctagt tcagctcggg gtgttggacg ggccgccgca cgacctccat cggttcgccg
     6601 cagtggcgcg gcaccgtcag ctcaccgagc cgggtgacct tgtactcggt cccgcactcc
     6661 gcgcagacga agaacacatc gaggtctccg acgtcctccg gctcctcctc gtgagcctgc
     6721 cccgccccgg cgccgatggc gcgcaggaag cggtatccca cgaacaggat caccccgccg
     6781 atggcgaacg cgatcagcac gcgcagcgcg aacggcacgg acccatcgta cgcgccgccc
     6841 gagctgtggc cgcgtggagg ttcctacagc cgttcgatga tcgtggcgtt cgccatgccg
     6901 ccgccctcgc acatcgtctg gaggccgtag cgcgcctcgc gacgctccag ctcgtgcagc
     6961 agggtggtca tcaggcgcgc gccgctcgcc ccgagcgggt gtcccagcgc gatcgccccg
     7021 ccgttcgggt tgacgttctc ctcgaaccag tcgtcgggga tgccgagctc cttcttccac
     7081 gccagcacca ccgtcgcgaa ggcctcgttg atctcgacca ccgagatgtc ctcgagcgac
     7141 agcccggcct tcgccagcac gcgctgcgtc gccgggatcg ggccggtgag catcgtgatc
     7201 gggtcgacgc cggccagggc gaacgcgtgg aaccgcgccc gcggccgcag gccgagctcc
     7261 tcggccttct cgcggctggt gatcacgagc gccgcggcgc cgtcggtgat ctggctggag
     7321 ttcgcggcgg tgacgacgcc gtcctccttg aacgccggcg ccagctcggc gatcttgtgg
     7381 cggttcggcg ggtagcggac gccctcgtcg gtgtccatca gctcgacccc gccgtcctcg
     7441 gtctgcacct tgaccggctg gatctcctga tggaacttcc cggcctggat cgccgcgccg
     7501 gcacgggtgt ggctgcgggc ggcgaactcg tcgagctcct ggcgggtcaa gctccagcgc
     7561 ttggcgatga tctcggcgct gatcccctgc gggacgagcc cgccctcgta gtggtccatc
     7621 agctgcgggc tgaacgggaa gcccgggccc tgcgaggcgg tcacgcccat cgggacgcgg
     7681 gtcatgtgct ccacgcccga ggcgatcacc acgtcgtagg cgccggcgat gacgccctgc
     7741 gcagcgaagt gggcggcctg ctggctcgag ccgcactggc ggtcgatcgt ggtgccgggc
     7801 acctcctccg gaaggccggc cgccagccag gcgttgcggg cgatgttcag cccttgctcg
     7861 ccggtctgcg agacgcaccc ggtgatgtgg tcctcgacca ggttggggtc gatcccgctg
     7921 cgttcgaccg cggacctcac cacctcggcc aggaggtcga cgggatgcca cccggagagc
     7981 ttgccgccgc gcttccccat cgggctcctc accgcctcga cgatgactgc ctcaggcatc
     8041 tcgggctcct ttcgagagcg tcgtggcgcc gaacgggtcc ggcctcggtg ccggccttgg
     8101 cgcattacct accgatcggt aacctcagta cgggacaggg tattcccggt caacgggagc
     8161 gtcaatccga gggacggtcg gcctcgcggt cggcctcccg caggctcctc ccccgctcga
     8221 gcatggccgc ccgcacccgg tcggccagcg cgggcatctc cttggccatg cgctggaact
     8281 cccgggcgaa gatcacgatc agccgcattg gggtgccagc gatcaccgag gccgtgcggc
     8341 gctcggtctg cagcagcccg atctccccga agaagtcgcc cgggcccagc gtggccacct
     8401 cggcgccgcc gtggaccacg ctcgcggacc ccttctcgat cacgaagaac tcgtaggcga
     8461 agcgcccctc gtcgaccagg tgcttgccct cggggacctc cacctcgtcg gcccaccgcg
     8521 ccaccgcctc ccggtccttc tgggagagcc ctgcgaacag cggaagagcc atcagccggt
     8581 ccgggtccat gtcgctcctc tcctcgcccc gggggggagc ctaccgccgc ccggtgaacg
     8641 cgaggaagcc g
//
LOCUS       DASPWO010000090         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_864107, whole genome shotgun sequence.
ACCESSION   DASPWO010000090 DASPWO010000000
VERSION     DASPWO010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864107"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VEO00_02050"
     CDS             complement(<1..182)
                     /locus_tag="VEO00_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HYU56819.1"
                     /translation="MQVNVIAAELYAPDPDPGVRVFDSRDAPADRDGLARLAERVRGA
                     DLAAPPVALPDFHHEGG"
     gene            complement(187..1365)
                     /locus_tag="VEO00_02055"
     CDS             complement(187..1365)
                     /locus_tag="VEO00_02055"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HYU56820.1"
                     /translation="MIGALREHLAARWPELRPGSPPLRGAILAGADKHPSAKVTFLFF
                     DDWGRAAVAVKVARDAHGERALATEHAALAHFWSTKSACVPANIPEPLLLERIHGRLA
                     LVMSALEGEPMMTRYYRPRHTSDPARVARDFEMAGEWLRRFQRETGAGRQELTPQTYR
                     RWATPILDRYRTEIGWGPAEAELFDAVLDRVADLAGCGIPFTGVHGDYWTGNLLVNGP
                     RVTGVIDWELGKPTGLPFSDLYKFPTSYGFYLDRAYPAGTTRIPGHAGREAARHRWSG
                     FGEWPNLVGFGYAYFGQGWFPDLVRRYVLDHFEHLGVPAAANAVFFPLFLAEQATALP
                     DPAFRNGYRALLAGFTAERSGGWLWEPDPAGPIDLDAVRQDRAAKPAAVAAAAHGEGV
                     "
     gene            complement(1430..>2044)
                     /locus_tag="VEO00_02060"
     CDS             complement(1430..>2044)
                     /locus_tag="VEO00_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56821.1"
                     /translation="EPAGLLLLPANGERLDGAVVPDHTAAALLATLAEGPFPAVAVPD
                     LGVWGHLAGGGRSGRMLERLAGAVAPGGWLYAGFANPWYPARFRLTRAMRLRRAVRIL
                     HRAGMGGTEVYLAFPDQTCPAYLVSKGGPAELDAFLRHLFLPYAGASQGWRGRVKQRV
                     LSLMRAAALVSPHSLKARFAPALAIVARRGGPGGQQPRGAERSG"
BASE COUNT          265 a          765 c          776 g          238 t
ORIGIN      
        1 ccgccctcgt ggtggaagtc gggcagggcc accggcgggg cggcgaggtc ggcgccccgg
       61 acccgctcgg cgaggcgggc cagcccgtcg cgatcggccg gcgcgtcccg gctgtcgaac
      121 actcgcacgc ctggatcggg atcgggggca tacagctcgg cggcgatgac gttcacctgc
      181 acgggctcag accccctccc cgtgcgcggc cgcggccacc gccgcgggct tcgcggcccg
      241 gtcctggcgg acggcgtcga ggtcgatggg tccggccggg tccggctccc acagccaccc
      301 gccggagcgc tccgcggtga accccgcgag cagggcgcgg tacccgttcc ggaacgcggg
      361 gtcgggcagc gcggtggcct gctccgcgag gaacagcggg aagaacacgg cgttcgcggc
      421 ggcggggacg cccaggtgct cgaagtggtc gagcacgtac cggcgcacga ggtccgggaa
      481 ccacccctgc ccgaagtacg cgtagccgaa cccgaccagg ttcggccatt ccccgaaccc
      541 gctccaccgg tgtcgggcgg cctcccggcc ggcgtgcccg gggatgcgcg tggtcccggc
      601 ggggtaggcc cggtccaggt agaacccgta ggaggtcggg aacttgtaca ggtcggagaa
      661 cggcaggccg gtgggctttc ccagctccca gtcgatgacc ccggtgaccc ggggaccgtt
      721 caccaggagg ttcccggtcc agtagtcgcc gtgcacgccg gtgaacggga tcccgcagcc
      781 ggcgaggtcc gcgacccggt ccagcaccgc gtcgaagagc tccgcttcgg cgggccccca
      841 cccgatctcg gtccggtagc ggtccaggat gggcgtggcc caccggcggt acgtctgtgg
      901 ggtcagctcc tgccggcccg ccccggtctc gcgctggaac cggcggagcc attccccggc
      961 catctcgaag tccctggcca cccgggccgg gtcgctcgtg tgccggggcc ggtagtagcg
     1021 cgtcatcatg ggctcgccct cgagcgccga catcacgagc gcgagccgtc cgtggatgcg
     1081 ctcgagcagc agcggctcgg ggatgttggc cgggacacag gccgacttcg tggaccagaa
     1141 gtgggccagc gcggcgtgct cggtcgccag ggcccgctcg ccgtgcgcgt cgcgggccac
     1201 cttcaccgcc accgcggcgc gcccccagtc gtcgaagaac aggaacgtga ccttcgcgga
     1261 agggtgcttg tccgcgccgg ccaggatggc gccccgaagc ggcgggctgc ccggtcgcag
     1321 ctccggccac cgagccgcca ggtgctcgcg cagcgcgccg atcatctgtt ctcactcctc
     1381 gcccggccgc ctgcggcggc ccggctcggc cagggggaat gcagcagcct catccgctcc
     1441 gctccgctcc gcggggctgc tgcccccctg gaccccctcg ccgggcgacg atggcgaggg
     1501 cgggggcgaa gcgggccttc aggctgtggg gggagaccag ggcggccgcc cgcatcaggg
     1561 acagcacgcg ctgcttcacg cgtccccgcc aaccctggga ggcgccggcg tagggcagga
     1621 acagatgccg gaggaacgcg tcgagctccg ccgggccgcc cttcgacacc aggtatgccg
     1681 ggcacgtctg gtcgggaaag gccaggtaga cctcggtccc gcccatgccg gcccggtgga
     1741 ggatccgcac ggcgcggcgc aagcgcatgg cccgggtcag gcggaaccgg gccgggtacc
     1801 aggggttggc gaacccggcg tacagccacc ccccgggggc cacggcgccg gccagccgct
     1861 cgagcatgcg tccggaccgg cccccgccgg ccaggtggcc ccacacgccg aggtcgggca
     1921 cggcgaccgc cgggaacggg ccctccgcga gcgtggccag cagggccgcc gccgtgtggt
     1981 cggggacgac ggcgccgtcc aggcgctcgc cgttcgccgg cagcagcagg agcccggccg
     2041 gctc
//
LOCUS       DASPWO010000091         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1037318, whole genome shotgun sequence.
ACCESSION   DASPWO010000091 DASPWO010000000
VERSION     DASPWO010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037318"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VEO00_02065"
     CDS             complement(<1..558)
                     /locus_tag="VEO00_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HYU56822.1"
                     /translation="MPEGDAVHLTARRLHAALADKVVTRSDLRSPRFATADLRGQTVT
                     EVVARGKHLLLRTDDGLTLHTHLKMDGVWRLYWPGERWREPTFQARAIVEVPDRQAVG
                     FRLAIVELLRTDREGDVLGHLGPDVLGPDWDPDEALRRLTGDPAREIGEALVDQRVMA
                     GPGNVYKSEALFLRGIHPRTPVGRVG"
     gene            572..1069
                     /locus_tag="VEO00_02070"
     CDS             572..1069
                     /locus_tag="VEO00_02070"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HYU56823.1"
                     /translation="MAATGRTRFRTVVRPAGPKGGSAKIEITPAQAKPLGVRRLSPVA
                     ITIRGHRFRTSMTPTGDGGFYMVLNRSMRETTSVGHGDRILVMVELDTEPRAVDVPPE
                     LARALRRSRRAAREFERIAPSHRREYAAYVDEAKRPETRERRARRSIEMLEELAAKQS
                     PSGRP"
     gene            complement(1076..>2327)
                     /locus_tag="VEO00_02075"
     CDS             complement(1076..>2327)
                     /locus_tag="VEO00_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYU56824.1"
                     /translation="RKEAEPVTAGALARFLPAWHGVGPAGPRSADADAVYRVISRLQG
                     APIPASALERQILPARLPGYSAAVLDQLCASGEVVWAGVGAIGPDDGWVALGLADRAP
                     LLLPDPPPVELSETAAAVREALSDRGALFFRQVVDATGSEEDTEVLLALWELVWAGLV
                     TNDTLAALRALTDRGGLTRRTPRRRRGPVFPPRSGPPAAAGRWSLLPPREPDPTRRKH
                     AIAAQLLERHGVVTRGAVASERIPGGFAAVYPVLKAMEEAGSCRRGYFVEGLGGAQFA
                     PPGAVDRLRVLAEPPPAVQTLVLAATDPANPYGAALPWPAKERERPAHRPGRKAGAVV
                     VLVDGELAVYVERGGRTLLTHVEDPEVLRPAVDALALAVRDGMLGKLTVERADGAGVF
                     DTPLARALTEAGFVPSTRGLRLRG"
BASE COUNT          310 a          860 c          905 g          252 t
ORIGIN      
        1 gccgacgcgg ccgacgggcg tgcgtgggtg gatcccgcgc aggaacagcg cctcgctctt
       61 gtagacgttc cccggaccgg ccatcacccg ctggtcgacc agcgcctcgc cgatctcccg
      121 tgccggatcg cccgtcaggc ggcggagcgc ctcgtccggg tcccagtccg ggccgaggac
      181 gtccggcccg aggtgcccga ggacgtcccc ctcgcggtcg gtgcggagca gctcgacgat
      241 agcgagccgg aacccgacgg cctgacgatc cgggacctcg acgatcgcgc gggcctggaa
      301 ggtgggctcc ctccagcgct cgcccggcca gtacaggcgc cacacgccgt ccatcttcag
      361 gtgcgtgtgc agcgtgaggc cgtcgtcggt gcgcaggagg aggtgcttgc cgcgggcgac
      421 cacctcggtg accgtctgcc cgcgcaggtc ggcggtggcg aaccgcggcg accgcaggtc
      481 cgagcgcgtc acgaccttgt cggccagcgc ggcgtgcagg cgccgggcgg tcaggtggac
      541 cgcgtcgccc tcaggcatgc aggatggagg ggtggcggcg acaggccgga cgcgattccg
      601 aacggtggtt cgtcccgccg ggccgaaggg cggctcggcg aagatcgaga tcacgccggc
      661 tcaggccaag ccgctcggcg tgcgcaggct gtcgccggtg gcgatcacga tccgcgggca
      721 caggttccgg acctcgatga ccccgaccgg cgacggtggc ttctacatgg tgctgaaccg
      781 gtcgatgcgt gagacgacca gcgtcggcca cggcgaccgg atcctcgtga tggtcgaact
      841 ggacaccgaa ccgagagcgg tcgacgtgcc cccagagctg gctcgcgcgc tgaggcgcag
      901 ccgccgcgcg gcgagggagt tcgaacgcat cgcgccctcg cacaggcggg agtacgcggc
      961 ctacgtggac gaggcgaagc gaccggagac gcgcgagcgg cgcgcgcggc gctcgatcga
     1021 gatgctcgag gagctcgccg cgaagcagtc cccctccggg cggccctgac accggctacc
     1081 cgcgcaggcg caggccgcgc gtggacggga cgaacccggc ctcggtcagg gctcgggcca
     1141 gcggcgtgtc gaacacgccg gcgccgtcgg cgcgctcgac cgtgagcttc ccgagcatgc
     1201 cgtcgcgcac cgcgagcgcc agggcgtcga ccgccggacg caacacctcg ggatcctcga
     1261 cgtgggtcag gagcgtccgc ccgcccctct ccacgtagac ggccagctcg ccgtccacga
     1321 gcacaaccac cgcgccggcc ttgcggcccg ggcggtgggc cggccgctcg cgctccttcg
     1381 ccggccacgg gagggcggcc ccgtacgggt tggcggggtc cgtcgccgcg agcaccagcg
     1441 tctggaccgc cggcggcggc tcggccagga cgcgcagccg gtcgacggcc ccgggtggcg
     1501 cgaactgcgc gccgccgagg ccctcgacga agtagccgcg gcgacagctc cccgcctcct
     1561 ccatcgcctt cagcacgggg tagacggcgg cgaacccgcc ggggatccgc tccgaagcca
     1621 ccgcgccgcg ggtgaccacg ccgtggcgct cgaggagctg cgcggcgatg gcgtgcttgc
     1681 ggcgggtggg gtcgggctcg cggggcggga gaaggctcca gcgtccggcg gctgccggcg
     1741 ggccggagcg gggcgggaag accgggcccc gacggcggcg gggcgtgcgt cgggtcaagc
     1801 cgccgcgatc ggtcagcgca cgcagggcgg cgagggtgtc gttcgtgacc aggccggccc
     1861 agaccagctc ccagagggcc agcagcacct ccgtgtcctc ctccgacccg gtcgcgtcca
     1921 cgacctgccg gaagaacagt gcaccgcggt cggacagggc ctcgcgcacg gccgccgccg
     1981 tctcggagag ctcgaccggc ggcgggtcgg ggagcaggag cggcgcccgg tcggcgagcc
     2041 cgagggccac ccagccgtca tccggcccga tcgcgccgac gccggcccag accacctcgc
     2101 cggacgcgca gagctggtcg agcaccgccg cggagtagcc ggggagacgg gccgggagga
     2161 tctggcgctc cagcgcggac gccgggatcg gcgcgccctg cagccgcgag atcacgcggt
     2221 agaccgcgtc ggcgtcggcg ctgcgcggcc cggcgggccc gacgccgtgc caggcgggca
     2281 ggaaccgggc cagggcgccg gccgtgaccg gctccgcctc cttgcgc
//
LOCUS       DASPWO010000092         3577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_948165, whole genome shotgun sequence.
ACCESSION   DASPWO010000092 DASPWO010000000
VERSION     DASPWO010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948165"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="VEO00_02080"
     CDS             complement(<1..1825)
                     /locus_tag="VEO00_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HYU56825.1"
                     /translation="MRRQRITFTTLLLATASILATAVTSFAAPLPGGTLDPLTVPKYV
                     DALPIPGVMPATSTDATIDYYEIAVRQFQQQVLPTGLPLTTVWSYGSVNHLGTFSYPA
                     FTIEATVGKPVKVKWMNQLVVDPVACAASPNPATDPACNFVPHLLPVDQTLHWANPPQ
                     DCTDGTPRPDCRGQSQLRYTGPVPVVTHLHGAHVQPDSDGYPEAWWLPAANNIPTGYA
                     TRGSNFDQIPGAPLEDGAALFQYGNDQRATALWFHDHSVGMTRSNVYAGPAGFYFLRG
                     GAADVSSGVAGGLPGGVYEIPLVIQDRSFNTDGSLFYPDSRVFFDGYAGPYIGDPLFA
                     SDAAPLHNPEFFGNTMVVNGKTWPFLNVEPRKYRLRILNGSDSRFLILEFPANVKAKF
                     TVIGTEGGFLPAPVVVSQLLVGPAERFDVIVDFTGLKPGAVFNLLNVGPDSPFGGPFP
                     ASGEADAKTTGQVMQLRVVALAAADTSAIPALPTITPVGAPTNVRQLSLNEAESALIC
                     VDKNNVFVPGVTPPVCGKRSAPLAPVMAQLGTIDPLTGLGIPKAFDDPITENVGLDST
                     EQWEISNFTADAHPIHLHLVMFQVVSRQPFGGVARVPEVWET"
     gene            complement(2223..2597)
                     /locus_tag="VEO00_02085"
     CDS             complement(2223..2597)
                     /locus_tag="VEO00_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56826.1"
                     /translation="MPLVARAGEFSLHHTHLLHSSAPNHGRDRRIGVGISYIPTRVPH
                     VGPRRLTASLVRGHDRYGHVDPEPRPRADFDAEARAAHAEACERLHHLPSRLARAQRL
                     RDEGKVLDRAPSSKPPTVPDRL"
     gene            3384..>3577
                     /locus_tag="VEO00_02090"
     CDS             3384..>3577
                     /locus_tag="VEO00_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYU56827.1"
                     /translation="MAGRLRGKVALITGAGSGIGAATAGLFAQEGAAVALADLGRESL
                     EPVAAQVKSAGGEALAMAVDV"
BASE COUNT          561 a         1118 c         1358 g          540 t
ORIGIN      
        1 ccgtctccca gacctcgggc acgcgcgcca cgccaccgaa cggctggcgg ctcaccacct
       61 ggaacatcac caggtggagg tggatggggt gggcgtcggc cgtgaaattg ctgatctccc
      121 actgctcggt cgagtcgagc ccgacattct cggtgatcgg gtcgtcaaag gccttcggga
      181 taccgagccc ggtcaggggg tcgatcgtgc cgagctgggc catgaccggg gcgaggggcg
      241 cggaccgctt gccgcagacc gggggagtga ccccgggcac gaagacgttg ttcttgtcca
      301 cgcagatcag cgcggactcg gcctcgttga ggctcagctg gcggacgttc gtcggcgcgc
      361 cgaccggggt gatggtggga agcgccggga tcgcgctcgt gtcggccgcg gcgagcgcca
      421 ccacccggag ctgcatgacc tggccggtcg tcttcgcgtc ggcctcgccc gacgccggga
      481 aggggccgcc gaacggcgag tcaggaccca cgttgagcag gttgaacacg gctcccggct
      541 tcaagcccgt gaagtcgacg atcacgtcga accgctccgc cgggccgacg aggagctgac
      601 tgaccaccac cggagcgggc aggaagccgc cctcggtgcc gatcacggtg aacttcgcct
      661 tcacgttcgc ggggaactcc aggatcagga accgcgagtc ggagccgttg aggatgcgga
      721 ggcggtactt ccgcggctcc acgttcagga acggccaggt cttcccgttc accaccatcg
      781 tattgccgaa gaactcggga ttgtgcaggg gcgcggcgtc gctcgcgaag agcgggtcgc
      841 cgatgtacgg ccccgcatac ccgtcgaaga acacccggct gtccgggtag aacagcgagc
      901 cgtcggtgtt gaacgaccga tcctggatga cgagcgggat ctcgtagaca ccgccgggga
      961 gcccgccggc cacgcccgag gacacgtcag cggctccgcc acggaggaag tagaagccgg
     1021 ccgggccggc gtacacgttc gagcgggtca tcccgacgct gtggtcgtgg aaccagagcg
     1081 ccgtggcccg ctggtcgttc ccgtactgga agagcgccgc tccgtcctcg agcggggcgc
     1141 ccgggatctg gtcgaagttc gatcccctcg tcgcatagcc agtggggatg ttgttcgccg
     1201 cgggcagcca ccatgcctcg gggtatccgt cgctgtcagg ctgcacgtgg gcgccgtgga
     1261 ggtgggtcac gacggggacc ggacccgtgt agcgcagttg actctgcccg cgacagtcgg
     1321 ggcgaggagt gccgtcggtg cagtcctggg ggggattcgc ccagtggagc gtctggtcca
     1381 cgggcaggag gtgcggcacg aagttgcagg ccgggtcagt tgccgggttc ggcgaagcgg
     1441 cacacgcgac cgggtcgaca accagttggt tcatccactt gaccttgacg ggcttgccga
     1501 cggtcgcctc gatcgtgaac gccgggtagc tgaacgtccc cagatgattc accgagccgt
     1561 agctccatac cgtcgtcaat ggaagacccg tgggcaggac ctgctgctgg aactgcctta
     1621 cggcgatctc gtagtagtcg atcgtcgcgt cggtgctcgt tgccggcatg acgcccggga
     1681 tcggcagcgc gtccacgtac ttcgggaccg tcaggggatc cagtgtcccg ccgggaagcg
     1741 gcgccgcgaa cgacgtcacg gcggtcgcga gtatggacgc tgtcgcaagc agcagtgtgg
     1801 tgaatgtgat tcgttgtcgc ctcatggttg tcctccctca ggtaaagagt ctgtcttggc
     1861 gccgatcggg atcgtcctga cccgtcctag gggctggata tcgcctgcgg atcgatcaag
     1921 cccgccccgt gcgcaccgag cgcgctgggc gatccgacgc cgcaattgat caagagccga
     1981 agagcactaa gaacagtgaa acacgggagg ggaggggcac caacccggtg aactccgggg
     2041 tagacggccg gaaaatcacg ggcgtcgata cggggaaatc ccgggtgcgc agccgcgggg
     2101 tggcaggcag cgtgacgaga tctctcgggg agcccaggaa tttcaaaagg ccctccggtc
     2161 ctcgtcaccc tgcccgcgcg tgccctggtg ggagggagaa tcgaccgcct gagcggccgc
     2221 cgtcaaagcc gatcggggac cgtcggaggc ttggaggacg gggcgcggtc gagaaccttg
     2281 ccctcgtcac ggagccgctg ggcgcgagcc agccgggagg ggaggtggtg caagcgctcg
     2341 caggcctccg cgtgggccgc gcgcgcctcc gcgtcgaagt cggcgcgcgg gcggggctcc
     2401 gggtcgacgt gcccgtaccg gtcgtggccg cgcaccaggg atgcggtgag gcggcgcgga
     2461 ccgacatgcg gcacgcgcgt ggggatgtag ctgatgccga cgccgatgcg ccggtctcgc
     2521 ccgtggtttg gcgccgagct gtgcaagagg tgcgtgtggt gcagcgagaa ctcgccggcg
     2581 cgcgccacca gcggcaccgc tcaggtcccg tccacgtcgg catcgacctc ctggccgttg
     2641 gacaggagat tgctcgcgga ccgatggctg acgtgcggga gctggcccca cccgtggctt
     2701 cccggcagcg cggcgacgca gcttggtctc cggcgtgctg tccgacaggg cgacccatgc
     2761 ggtgacgtgc tggtccgccg gatcgaggcc gaagtacgtc ccgtcctggt gccaggagac
     2821 gaacgtcgcg tcgccgggct ccttgatcca catcgtgagg tggtagacga ggagatccgg
     2881 cccgatcaga tcctcgacgg cggccagcac cgccgggtgg cggaggaggt cgtcgagcca
     2941 cgggaacagt atgggtcttg gcccggagct ggcccgggag ctgcccgagc ggccgtccct
     3001 gcgaggcctc gaaggcctcg agcctcctgc gacagtcgcg cgcctcgagc ggggacagcg
     3061 caggccgctg gccaggagcg cctcggcctc gaggggcggc ggagaaggat cgaggttggc
     3121 gcagggcact tgggcgtttg accgttgcca accgagccgg ggataatgtt ccaggctcga
     3181 atcttgccac gcgggtgcag cgcgtgagtt gcggtaaggt gaagtggctg gcgcgcggcg
     3241 gcggggacgt ccgagagtcc gcgtcgcttc ccgggccgac ggggatcatg accgcatcac
     3301 gcgaggagtg accgcgcgat tcgtcgggat tcgagcgacg gcggcggtga gatcggggca
     3361 ggggtgttca cggaggtgtc cagatggcgg gccgacttcg gggcaaggtg gctctgatca
     3421 cgggcgcggg atcgggcatc ggcgcggcga cggccgggct gtttgcgcag gaaggcgcgg
     3481 cggtggcgct cgccgacctg gggcgggaaa gcctggagcc cgtcgcggcc caggtcaaga
     3541 gcgccggcgg cgaggcgctg gcgatggcgg tcgacgt
//
LOCUS       DASPWO010000093         6509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_948198, whole genome shotgun sequence.
ACCESSION   DASPWO010000093 DASPWO010000000
VERSION     DASPWO010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948198"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1509)
                     /gene="sufB"
                     /locus_tag="VEO00_02095"
     CDS             complement(130..1509)
                     /gene="sufB"
                     /locus_tag="VEO00_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HYU56828.1"
                     /translation="MSKGYRFGWKDPSHSVFTPKKGLSQAVVEEISGLKDEPDWMRKF
                     RVKAYKHFEKRPMPWWGADLSGIDFDDIYYFIRSTEKQATSWADLPEDIRGTWDKLGI
                     PEAEKKFLGGVSAQYESEVVYHKIKDELDAMGVIFTDMDTGLREHPEIVKEYFGKLIP
                     PNDNKFAALNSAVWSGGSFVYVPEGVHVDIPLQAYFRINAQNMGQFERTMIIAEEGSY
                     VHYVEGCSAPIYTSDSLHSAVVEIIVKKGARVRYTTIQNWSSNVYNLVTKRAAAYEDA
                     VMEWIDGNIGSKVTMKYPSIYLLGKGARGEVLSVAFAGQGQHQDAGGKAIHVAPHTSS
                     VITSKSVSKDGGRTGYRGLVRVEPGADHAKSMVRCDALILDERSRSDTYPYVEVEEES
                     AQLGHEASVARIGEDQLFYLMSRGLSEAEASAMIVNGFIEPITRELPMEYAVELNRLI
                     ELQMEGAIG"
     gene            complement(1648..2490)
                     /locus_tag="VEO00_02100"
     CDS             complement(1648..2490)
                     /locus_tag="VEO00_02100"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HYU56829.1"
                     /translation="MAEYANPEALVETDWLEEHLNDATVRVIDVDEDTTAYEKGHIPG
                     AVGWNWNTDLHDPLRREYVDQEGLARLLSKAGVAADTTVVLYGGNNNWFASYAYWILK
                     YLGFDGVKLLNGGRKKWELESRELSQEVPAYAGTGLTVSGAVRPEIRAFRDEVLEKLG
                     GGTRFVDVRSPEEFRGEKLAPDHLPQEQAQVPGHIPGAANIPWVKAANDDGTFKSADE
                     LRALYQEEGVTGDGETIAYCRIGERSSHTWFALKELLGYGNVKNYDGSWTEYGSLVGV
                     PVER"
     gene            complement(2830..3246)
                     /locus_tag="VEO00_02105"
     CDS             complement(2830..3246)
                     /locus_tag="VEO00_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HYU56830.1"
                     /translation="MKEERPTITDERDDSGVTPAADLAPAADARARAEANGDGALTRE
                     AVLEALKEVLDPELGINIVDLGLVYDVSIEGDTVHITYTLTTMGCPIGPLIEAQMQQL
                     LAGVEGVKNVEAEMVLRPAWTPEMMSEEAKAALGYF"
     gene            3372..4001
                     /locus_tag="VEO00_02110"
     CDS             3372..4001
                     /locus_tag="VEO00_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU56831.1"
                     /translation="MRILIVDDHPVTRDGLRTALSANDEVELVGEATTGEEAIEAVDK
                     LKPDIVFMDVRMPGMNGIEATKVIRQEHPETRVILFTVDESRVSISEAIQAGVSGYLL
                     KDASIDELMHAARFAMDGKAVIHPALTQAFIEEVRVTQDRRPETTLSKRETEILQKVA
                     YGATTKEVADSLQISPHTVKTHLERIFEKLGANDRAQAVAIALRKGIVE"
     gene            complement(4041..5030)
                     /locus_tag="VEO00_02115"
     CDS             complement(4041..5030)
                     /locus_tag="VEO00_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU56832.1"
                     /translation="MSFTVLETVLLGRSPYLSPWAGGGPEDWAHARRAMERANVQHLA
                     DRPLDRLSGGERQRVVLAQALAQDAPVLLLDEPTTHLDLHHVLDVLTVVGRLARREGR
                     AALAVFHDLNLAAASCDRLVALAVGEVVAAGTPEEVISRALLAEVYGVEAEVIPHPLT
                     GRPTVILGGTLPAAAPVPGSPRAHVVGGAGRGAPLMRALAERGYEVTAGVLHATDTDA
                     VVAERLNLLRVTVPPFSPIDPAAAEECLALARTAEVIYLLDAPYGPGNVENLRVVREA
                     QRAGRRVVVLEQVPIGERDFTRGEATRLWEEIGAHAERVRTYEELLARAAAKG"
     gene            complement(5054..5299)
                     /locus_tag="VEO00_02120"
                     /pseudo
     CDS             complement(5054..5299)
                     /locus_tag="VEO00_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(5334..6389)
                     /locus_tag="VEO00_02125"
     CDS             complement(5334..6389)
                     /locus_tag="VEO00_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HYU56833.1"
                     /translation="MRRRRGLFAWLALVLAGSVLAGVAIGAVSIPPATTLRLFAWKLG
                     LAGFPAGVPSSTAVILFQLRLPRVVLAGEVGAALAASGAVFQALYRNPMADPAIVGVS
                     SGAALGAVAVILLGGGAALGGLGVPAAAFAGALATAFLVYRLARIGPSIQVATLLLSG
                     IAVAAVISAVISLVMTFSGQQVRSIYFWLLGGLGARGWDATIVSAPLVAIGLAAAALA
                     ANHLNLSLLGEERAGQLGLDVERFKRSMLATGALLAGAAVSVSGLIGFVGLMTPHILR
                     RLVGPDHRRLLPAVVLAGASFMILADLAARSVLSPEEIPVGAVTALLGGPFFLWLLRR
                     ERGGFGGVARAGERRTR"
BASE COUNT         1008 a         2389 c         2167 g          945 t
ORIGIN      
        1 cccgacctaa agtctactga attagtcgga ttcagcggtg acggttcgcc ggtggcgcca
       61 gcgcgatgaa ggcgaagaca ctcaggaacg ggcgtggctg caggacgcgc gtgatcgccc
      121 gcgagccggc tagccgatcg cgccttccat ctgcagctcg atcaggcggt tcagctcgac
      181 ggcatactcc atggggagct cgcgggtgat cggctcgatg aagccgttca cgatcatcgc
      241 gctggcttcg gcctcggaga ggccgcggct catcaggtag aagagctggt cctcgccgat
      301 gcgggcgacg ctcgcctcgt ggccgagctg cgccgactcc tcctcgacct cgacgtacgg
      361 gtaggtgtcg gagcgggacc gctcgtcgag gatcagcgcg tcgcagcgca ccatcgactt
      421 cgcgtggtcg gcgccgggct cgacccggac cagcccgcgg tagccggtcc gtccgccgtc
      481 cttcgacacc gacttgctcg tgatcacgct cgaggtgtgt ggcgcaacgt ggatcgcctt
      541 gccgccggca tcctggtgct gaccctggcc ggcgaacgcc accgacagca cctcaccgcg
      601 cgcgcccttg ccgagcaggt agatggacgg gtacttcatc gtcaccttcg agccgatgtt
      661 gccgtcgatc cactccatca ctgcgtcctc gtacgcggcg gcccgcttcg tcaccaggtt
      721 gtagacgttt gacgaccagt tctggatcgt cgtgtagcgc acccgcgcgc ccttcttgac
      781 gatgatctcg accaccgcgg agtgcagcga gtccgacgtg tagatcggcg ccgagcagcc
      841 ctcgacgtag tgcacgtacg agccctcctc ggcgatgatc atcgtccgct cgaactgccc
      901 catgttctgc gcgttgatcc ggaagtaggc ctgcagcggg atgtcgacgt gcacgccctc
      961 ggggacgtac acgaacgacc cgccggacca caccgccgag ttcagcgccg cgaacttgtt
     1021 gtcgttcggc gggatcagct tgccgaagta ttccttgacg atctcggggt gctcgcgcag
     1081 cccggtgtcc atgtccgtga agatcacgcc catggcgtcc agttcgtcct tgatcttgtg
     1141 gtacacgact tcgctttcat attgtgcaga gacgccgccg aggaacttct tctcggcctc
     1201 gggaatcccg agcttgtccc aggtcccccg gatgtcctcg gggaggtccg cccaagacgt
     1261 agcctgcttc tccgtggagc ggatgaagta gtagatgtcg tcgaagtcga tcccggacag
     1321 gtcggccccc caccacggca tgggccgctt ctcgaagtgc ttgtaggcct tcacccggaa
     1381 cttgcgcatc cagtccggct cgtccttcag gcccgagatc tcctcgacca cggcctggga
     1441 cagccccttc ttgggcgtga agacggaatg cgaggggtcc ttccagccga accggtaccc
     1501 cttgctcagt tccttgagat gctcttcctg ggtggtggac acgatccatc aacccctcgt
     1561 cactgcgaag gtcctcatca ggaagtctac ccgctcgggg cggtaaagtc gacctggcag
     1621 ataggatttg accaggcgcc gatgccctta ccgctcgacc gggaccccca ccagcgagcc
     1681 gtactcggtc cacgacccgt cgtagttctt cacgttcccg tagcccagca gctccttcag
     1741 ggcgaaccag gtgtgcgagg agcgctcccc gatccggcag taggcgatcg tctcgccgtc
     1801 gccggtcacc ccctcctcct ggtagagcgc ccgcagctcg tcggccgact tgaacgtgcc
     1861 gtcgtcgttc gcggccttga cccatgggat gttcgccgcc ccggggatgt gcccgggcac
     1921 ctgggcctgc tcctggggca ggtgatccgg cgccagcttc tccccgcgga actcctccgg
     1981 cgagcggacg tccacgaacc tcgtgccacc gcccagcttc tcgagcacct cgtcgcggaa
     2041 cgctcggatc tcggggcgca ccgcccccga gacggtcagc cccgtcccgg cgtacgcggg
     2101 gacctcctgc gacagctcgc ggctctccag ttcccacttc ttgcggccgc cgttcagaag
     2161 cttgacaccg tcgaacccga ggtacttcag gatccagtag gcgtaggagg cgaaccagtt
     2221 gttgttgccc ccgtacagca ccaccgtggt gtcggcggcc acaccggcct tggacagcag
     2281 cctcgcgagg ccctcctggt cgacgtactc gcggcgcagc gggtcgtgca agtccgtgtt
     2341 ccagttccag ccgacggccc cggggatgtg ccccttctcg taggcggtgg tgtcctcgtc
     2401 cacgtcgatc acccgcaccg tcgcgtcgtt cagatgctcc tcgagccagt cggtctcgac
     2461 gagcgcctcg gggttcgcgt actccgccat cgctcctccc tccctaccgc tctcaccaac
     2521 ctgccgcgct caccaacaga agagggccgc cgatcgcggc cctctcgcgt gcccggtggt
     2581 tcggtcctcg gtgctaccgc acgccgatcc ccccgcccgc gatcgggcgc cacgcacggg
     2641 tacagcagat gcgggaaggg atcatcgccc cgacgagccc cgcgcagcgc gagcggatca
     2701 tgcgctattt ccgatctttc cactcggatt tgcagatgcg attagaccgc ggccgccgga
     2761 cgttgtcaac cggaaagacg gcaagacggc cttgcggggg tgcacggggg gcggagcccc
     2821 ccgggtgcat cagaagtagc cgagggcggc cttggcctct tcggacatca tctccggggt
     2881 ccaggccgga cggaggacca tctcggcctc gacgttcttc acgccctcca cgccggccag
     2941 gagctgctgc atctgggcct cgatcagcgg gccgatcggg cagcccatgg tggtcagcgt
     3001 gtaggtgatg tggacggtgt ccccctcgat cgaaacgtcg tagacgagcc ccaggtcgac
     3061 gatgttgatc ccgagctcgg ggtcgaggac ctccttcaac gcctccagca ccgcctcccg
     3121 cgtcagggcg ccgtcgccgt tcgcctcggc gcgcgctcga gcgtcggccg ccggtgcgag
     3181 atcggctgcc ggggtgacgc cggaatcatc ccgttcgtcg gtgatggtcg gccgttcttc
     3241 cttcatccgc ttatccttcg cgaccagtcc agggagtatc ctacccatgt cgtagggaat
     3301 gccgccgatg ggatcacggg cgccgtgacc gtccgcgcgc cggcaacggg tcgagaggag
     3361 gccgcccaag catgcgcatt ctgatcgtcg atgaccatcc ggtgacgcgg gacggcctgc
     3421 ggaccgcgct gtcggcgaac gatgaggtcg agctggtggg cgaggcgacg accggcgagg
     3481 aggccatcga agccgtcgac aagctcaagc cggacatcgt cttcatggac gtgcgcatgc
     3541 ccggcatgaa cgggatcgag gcgaccaagg tgatccggca ggagcacccc gagacgaggg
     3601 tcatcctgtt caccgtcgac gagtcccggg tcagcatctc cgaggcgatc caggccgggg
     3661 tctccggcta cttgctgaag gacgcctcga tcgacgagct gatgcacgcg gcccggttcg
     3721 ccatggacgg caaggccgtg atccacccgg cgctgacgca ggccttcatc gaggaggtcc
     3781 gcgtcacgca ggaccgtcgc cccgagacca cgctctcgaa gcgcgagacc gagatcctgc
     3841 agaaggtcgc ctacggcgcg acgacgaagg aggtcgccga ctccctgcag atctcgccgc
     3901 acactgtgaa gacccacctg gagcggatct tcgagaagct cggcgcgaac gaccgggcgc
     3961 aggcggtggc tatcgcgctg cgcaagggca tcgtcgaata acacgggggc tccgcccccg
     4021 tgtgcaccgg aacggcacgg ctagcccttc gccgccgcgc gcgccagcag ttcctcgtac
     4081 gtgcggacgc gctccgcgtg agcgccgatc tcctcccaca gccgcgtggc ttcgccacgc
     4141 gtgaagtccc gctccccgat cggcacctgc tccagcacca ccactcgcct ccctgcccgc
     4201 tgggcctcgc ggaccacgcg caggttctcc acgtttcccg gaccgtacgg ggcgtcgagc
     4261 aggtagatca cctccgccgt ccgggcgagc gccaggcact cctcggccgc ggcgggatcg
     4321 atcgggctga acggcgggac cgtgacccgc agcaggttca gccgctcggc aacaaccgca
     4381 tcggtgtcgg tggcgtgcag cacgcccgcc gtgacctcgt acccgcgctc ggcaagggcc
     4441 cgcatcaggg gcgcgccgcg cccggcaccg ccgaccacgt gcgcccgcgg cgaccccggt
     4501 accggagcgg cggccgggag cgttccgccg aggatcacgg tcggccggcc ggtgaggggg
     4561 tgcgggatga cctccgcctc caccccgtac acctcggcca gcagcgcgcg cgagatcacc
     4621 tcctccggcg tgccggcggc caccacctcc cccacggcga gcgcgacgag ccggtcgcag
     4681 gacgcggcgg ccagattgag gtcgtggaag acggcgagcg ctgcccgtcc ctcccgccgc
     4741 gctagtcggc caaccaccgt caggacgtcg aggacgtgat gcaggtcgag gtgggtggtc
     4801 ggctcgtcga gcagcagcac cggcgcgtcc tgggccagcg cctgagccag gaccacgcgc
     4861 tggcgctcgc cgccggaaag ccggtcgaga gggcgatcgg ccaggtgctg cacgttcgcg
     4921 cgctccatgg cccgccgggc gtgcgcccaa tcctccggcc cgccgccggc ccacggcgag
     4981 agatacggcg accggccgag gagcaccgtc tcgaggacgg tgaacgacac ccccggcgcg
     5041 atctcctggg cacgacggcg acctgccggg ccgcggcacg ggggcgcagc gtgtaggggt
     5101 cctgcccgag cagccgcacg gtcccacgct cgggccgcag cccgcgcgag gccacccgca
     5161 cgagcgtggt cttccccgac ccgttcggcc cgatcaggcc gacgacctct cccgcgtcca
     5221 ccgtgagcga gacgccgcgc agcaccggca ggccgccgta gccggcccag acgtctcgca
     5281 cctccagcgc ggccgtcacg cgccgttcac cgtgtggggt ccgctcgcca tgcttatcgg
     5341 gtccggcgct ccccggcgcg agccacgccc ccaaaccccc cgcgctcgcg gcgcaacagc
     5401 cagaggaaga aggggccgcc gaggagcgcg gtgaccgccc cgaccgggat ctcctccggg
     5461 gacaggacgc tccgcgcagc caggtcggcc aggatcatga acgacgcgcc ggcgaggacc
     5521 accgccggga gcaaccggcg gtggtcgggc ccgaccagcc ggcgcaggat gtgcggggtc
     5581 atcagcccga cgaacccgat caggcccgat accgacaccg ccgccccggc gaggagcgcc
     5641 ccggtggcga gcatcgagcg cttgaagcgc tccacgtcga gcccgagctg gccggcccgc
     5701 tcctcgccga gcagcgacag gttgaggtgg ttcgcggcga gcgcggcggc ggcgagcccg
     5761 atcgcgacga gcggggccga cacgatcgtc gcgtcccacc cccgcgcacc gaggccgccc
     5821 aggagccaga agtagatcga gcggacctgc tggccggaga acgtcatcac cagcgagatc
     5881 accgccgaga tcacggcggc cacggcgatc cccgagagca acagcgtcgc cacctggatc
     5941 gacggcccga tccgcgccag ccggtacacg agaaaggccg tggccagggc gccggcgaac
     6001 gccgcggccg ggacgcccag gccaccgagc gccgcgcccc cgccgagcag gatcacggcg
     6061 acggccccca gcgctgcccc ggaggacact ccgacgatgg ccgggtcggc catcgggttc
     6121 cggtagagcg cctggaacac ggcgcccgag gcggccaggg cggcgccgac ctcgccggcg
     6181 aggaccaccc gcgggaggcg cagctggaac aggatcaccg ccgtcgagga cggcaccccg
     6241 gccgggaagc ccgccagccc gagcttccag gcgaacaggc gcagcgtggt cgcgggcggg
     6301 atcgagaccg caccgatcgc gacgccggcg agcaccgacc cggccagcac cagggccagc
     6361 caggcgaaga ggccgcgcct gcgccgcacc cgactccgcc ccgtctcgcc gacctcgaag
     6421 cgctccgccg tccgcgctac gcggccgggt ggagggcgtc ggccaggtcc tccagcccgt
     6481 ccaccacgcg cggtcccggc cgggagatc
//
LOCUS       DASPWO010000094         7282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_99438, whole genome shotgun sequence.
ACCESSION   DASPWO010000094 DASPWO010000000
VERSION     DASPWO010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99438"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..579)
                     /locus_tag="VEO00_02130"
     CDS             complement(103..579)
                     /locus_tag="VEO00_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56834.1"
                     /translation="MRFGTSWIALLVALALSAAACDGGSVTVNPPSDTSSATTPPAPT
                     TTEPTPSPTASPPPTAAPISPLAPTQPEVVAIGLYQAWGADDRDLALAYAEAEAVDAL
                     FDEPFIPEAFFAGCSREGDVGYLCQFNAPSQAIMFHVLGSAANGYRVQSIEIGSAG"
     gene            complement(629..1288)
                     /locus_tag="VEO00_02135"
     CDS             complement(629..1288)
                     /locus_tag="VEO00_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYU56835.1"
                     /translation="MEIAPKRTFAAALDWPGWCRPGVDSDAALAALFENGPRYGRAVA
                     SARLGFRPPRDPSALRVVQRVKGNATTDFGAPGVEARSDRRPLDDAERRRSERLLRAC
                     WRALDQAAEGAAGRELRKGPRGGGRSLAGIVRHVVEAEEGYLNALGWKPPKAGRGAFA
                     ERMADVRGAVLEGLAASARGEIAPRGPRGGVRWRPRYFVRRVAWHALDHAWEIEDRMM
                     G"
     gene            1401..2351
                     /locus_tag="VEO00_02140"
     CDS             1401..2351
                     /locus_tag="VEO00_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56836.1"
                     /translation="MGVLVAVVLLLLGMAFLEVGAQLAVGGVQALAVATGVSAFALGV
                     VLFGIDTESLGSTVVAAGRGETALGAGVAFGTIVFTFGAAFGVALLLSRRPVAAPAPA
                     KVLAPAIGLVGVTLTVTDLVVTRAEGLALVLLYVAYLSSVVVGARHERAAPIAGGSDD
                     AAGGTSGRGRLGWALARTAVGLVALYGGAWLMVEGGLRVLRLSDLTAGFVGAALVGVL
                     ASLDEVVLEMMAVRRGSDGLATGNLLGTLAGFTMLAPGVAAIVHPLTLDGAVSATLIA
                     AAILYVVVAVTFLLRGRAGRLLGAFLLVGFAGWAIAAVRL"
     gene            complement(2362..3036)
                     /locus_tag="VEO00_02145"
     CDS             complement(2362..3036)
                     /locus_tag="VEO00_02145"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYU56837.1"
                     /translation="MTDHLDVVFLDVGGPLYADDTYYRSLLAAIREVDPSVTGEAYAE
                     VYDACRRAQDGSFRTRLTRTLLGPEASVEEVAARAERHWLYPPEALVPDVVPALEALA
                     GRYRLGVLANQTGRIREVMRRDGIDRFFELWNVSAELGVEKPDPRIFRRALDDAGVPP
                     GRAAMVGDRLDNDVRPAKAAGMRGVWLLRGEAPDDPTPEQLDEPDAVIRSLGELPDAL
                     ERLRHR"
     gene            complement(3101..3937)
                     /locus_tag="VEO00_02150"
     CDS             complement(3101..3937)
                     /locus_tag="VEO00_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HYU56838.1"
                     /translation="MATIDLHTHSTASDGTFSPTEVVRLALERGVSLLALSDHDTTAG
                     LDEAADAAAGTGLELVPATEFSTVYEGGSIHVLAYWIDAANGEFQAELDRLRDDREWR
                     AREIVKRLNAMGHGIGYERVRELAAGNVVARPHIAQAMLEAGLVERYEDAFTAAFIAD
                     GGAAHVEKHALHPVDALYLIHRAGGVCVVAHPGMWSDADPVPDSLIERMAGEGLDGIE
                     ADHPDHTPEQRERYRAMARRLGLPFTGSSDCHGTRFDPVRLGTVTTDPAEFAKLRARA
                     AR"
     gene            complement(4019..5080)
                     /locus_tag="VEO00_02155"
     CDS             complement(4019..5080)
                     /locus_tag="VEO00_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56839.1"
                     /translation="MRRPILLVVVALLLAAVGAAPASAGPAGPFSRTVPSPPIKGTWE
                     RPFHASTSVLFKENLFRLWYVSVPKEGGKNGVGYAASSEGGTDWTTRSGAPLPGLFSN
                     KPWDDGGIVDISVLFAENRFRMYFTATNTSGRFRVGRAISQDGLHWTTRQSPVWSGDT
                     GEFSGNGPLQLSVIFKGGKYRMWFAATNNDGRGAIGYAASTDGLSWNSQPEPVLTGRK
                     GIPWEADFGLFQPKALFDPAANRYRLWYVGCCLQNDDPGDPFTTVNGFGVASSSSAAG
                     GWTHGSDVPVVPWDFTTNGQRDCDLQSPSVVLKGGTFRMLFVQFRYDESGDDCYVFQG
                     GTLVETHFSTRILVGTAPQ"
     gene            complement(5126..6280)
                     /locus_tag="VEO00_02160"
     CDS             complement(5126..6280)
                     /locus_tag="VEO00_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019544051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU56840.1"
                     /translation="MDLELSDEQQLIRKTAREWVDKEVVPRARDSDRAERLDPELVRQ
                     LGELGFLGAVIPELYGGMGLDNLSYCLIVEELGRGDSSVRGVVSVNVGLVGKTLYRFG
                     TEDQKRAWLTPLAAGEILGCFGLTEPGVGSDAAALTSRAERDGGDWLISGQKQFITNG
                     NDAAVALVFARTGGEGQKGITCFLVPTDVPGFSSRPVKGKLGLRAQDTAELFLDRVRV
                     PDANRLGDEGQGFSVAMSALDSGRMSLAAGCVGIGQGCLEASIAYARERRAFGRPIAR
                     FQLVQELLADMAVEVDAARLLTWRVAALADAGRGHTLESSYAKFFASEMAVRAANAAV
                     QVHGGYGYVDEYPVGKFLRDARVTTLYEGTSQIQKLIIGRALTGESAFGG"
     gene            complement(6727..>7282)
                     /locus_tag="VEO00_02165"
     CDS             complement(6727..>7282)
                     /locus_tag="VEO00_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HYU56841.1"
                     /translation="APRVARKLLSPGLSSGWGIRTLAARQVPYDPIGYHTGTVWPHDN
                     ALIAHGLKRYGLDHEAMQVIDQLSLAGMFFPYARYPELFCGFSAEEVPVPVQFPVACR
                     PQAWSAGAPLLMLRTYGGITADAPEGVLNLIRPHLPRWLERIEVHGLRVGDTRLDISF
                     QSHDGVTATQVPRKEGDIDVLIRH"
BASE COUNT          945 a         2681 c         2607 g         1049 t
ORIGIN      
        1 cgagcgagtt cttcttcgcc gcctcgcgcg tccccgccct gcgcatccgc gacttcaact
       61 tcaccggcaa gagcgaccac tagccggtcc cggccggacc ccctaccccg ccgagccgat
      121 ctcgatcgat tggacgcggt agccgttcgc cgcgctgccg aggacgtgga acatgatggc
      181 ctgcgacggt gcgttgaact gacacaggta gccgacgtcc ccctcccgcg agcagccggc
      241 gaagaacgcc tcggggatga acggctcgtc gaacagcgca tcgacggcct ccgcctcggc
      301 gtaggcgagc gcgaggtccc ggtcgtcggc accccaagcc tgatagagcc cgatcgccac
      361 gacctcgggc tgggtcggcg ccagcggcga gatcggcgct gccgtgggag gcggactggc
      421 ggtcggcgag ggggtcggct cggtcgtggt gggagcgggt ggggtcgttg cggacgacgt
      481 gtcgctcggg gggttcaccg tgacggagcc accgtcacag gcagcggcgg agagggccag
      541 cgcgacgagc agcgcgatcc acgacgtgcc gaatcgcatc gcgccacgac tatagccctc
      601 ggccgacgcg cccatcgtgc gacgggcgtc agcccatcat ccggtcctcg atctcccagg
      661 cgtggtccag cgcgtgccag gcgaccctgc ggacgaagta gcgcggcctc cagcggacgc
      721 ccccgcgcgg gccgcgcggg gcgatctcgc cgcgcgccga ggcggccagg ccttccaaca
      781 ccgcgccgcg cacgtccgcc attcgttccg cgaacgcacc gcgacccgcc ttgggcggct
      841 tccaccccag agcgttcagg tatccctcct cggcctcgac cacgtgccgg acgatgcccg
      901 cgaggctccg gccgccgccg cgcgggccct tccggagctc cctgccggcg gcgccctccg
      961 cggcctggtc gagggcgcgc cagcaggcgc ggaggagccg ctctgagcgc cgccgctccg
     1021 cgtcgtccag aggccgccgg tcgctcctcg cctcgacgcc cggcgctccg aagtcggtgg
     1081 tggcgttccc cttcacccgc tgcacgaccc gcagcgccga cgggtccctc ggggggcgga
     1141 acccgagccg ggcgctcgcg accgcccgtc cgtagcgggg accgttctcg aacagggctg
     1201 cgagcgccgc gtccgaatcc accccgggcc ggcaccagcc tggccagtcg agggccgcgg
     1261 cgaaggttcg cttcggcgcg atctccaagt agacgtcgat ctcccgacga tagcgggcga
     1321 tacgtttcgc cgccgttcat cgtccagccg gacccgtagc ccgcgcgccc cggccccgct
     1381 ccgcccgtat cctcccggcc gtgggtgtcc tcgtagcggt ggtgctgctc ctgctcggga
     1441 tggccttcct cgaggtcggc gcgcagctcg cggtcggcgg ggtgcaggcg ctcgccgtcg
     1501 ccaccggcgt ctccgccttc gcgctcggcg tcgtcctgtt cggcatcgac acggagagcc
     1561 tcggcagcac ggtcgtcgcg gcggggcgcg gcgagaccgc cctgggggcc ggcgtcgcct
     1621 tcgggacgat cgtgttcacg tttggggcgg cgttcggggt cgccctcctg ctctcgcgcc
     1681 ggcccgtcgc cgcgccggcg ccggcgaagg tgctcgcccc ggcgatcggc ctggtcggcg
     1741 tgacgctgac ggtgacggac ctggttgtga cccgggcgga gggcctggcg ctggtattgc
     1801 tctacgtcgc ctacctctcc tccgtcgtcg tcggggcccg gcacgagcgc gccgccccga
     1861 tcgccggcgg gtcggacgat gcggccggcg gcacttccgg ccggggccgg ctggggtggg
     1921 ccctggcgcg caccgccgtc gggctggtcg ccctgtatgg cggggcctgg ctgatggtgg
     1981 agggcggcct gcgcgtgctg cggctctccg acctgacggc ggggttcgtg ggcgcggcgc
     2041 tcgtcggcgt cctggccagc ctggacgagg tggtgctgga gatgatggcc gtccgccgag
     2101 ggtcggacgg cctggcgacc gggaaccttc tcgggaccct cgccggcttc acgatgctcg
     2161 ctcccggggt ggccgccatc gttcacccgc tcaccctcga cggagccgtc tccgcgacgc
     2221 tgatcgcggc ggccatcctg tacgtcgtcg tcgccgtgac cttcctgctg cggggacggg
     2281 ccgggcggct cctcggggcg ttcctgctgg tggggttcgc cgggtgggcc atcgcggcgg
     2341 tccggctgtg acggtgacga gctagcggtg gcgcaggcgc tccagcgcgt ccgggagctc
     2401 gccgagggac cggatcacgg cgtcgggctc gtcgagctgc tctggggtcg ggtcgtcggg
     2461 cgcctcgccg cgcagcagcc acactccgcg catcccggcc gccttcgccg gacggacgtc
     2521 gttgtccagg cggtcgccga ccatcgcggc ccgtcccgga ggcaccccgg catcatccag
     2581 cgcgcgccgg aagatgcgcg ggtccggttt ctccaccccg agctccgccg agacgttcca
     2641 cagctcgaag aaccggtcga tcccgtcacg gcgcatcacc tcccggatgc gcccggtctg
     2701 gttcgccagg acgcccaggc gatagcgccc ggcgagtgcc tcgagcgcgg gcacgacgtc
     2761 gggcaccagg gcctcgggtg ggtacagcca gtggcgttcg gcccgggcgg cgacctcctc
     2821 gaccgacgcc tccggcccga gcagggtcct cgtcaggcgg gtgcggaacg atccgtcctg
     2881 ggcgcgacgg caggcgtcgt agacctcggc gtaggcctca ccggtgacgg agggatccac
     2941 ctcccggatc gccgcgagca gcgatcggta gtaggtgtcg tcggcgtaga gcggcccgcc
     3001 gacgtcgagg aagaccacgt cgaggtgatc ggtcatgggt ccattggggg ggtccactcg
     3061 ccgtacgtgc tcgcttcgag cgtggaccgg tgccggtgga tcagcgagcg gcccgggccc
     3121 gtagtttcgc gaattccgcc ggatccgtcg tcacggtgcc cagccgcacc gggtcgaacc
     3181 gggtgccgtg gcagtccgag gacccggtga acgggaggcc gagccggcgg gccatcgccc
     3241 ggtaccgctc gcgctgctcg ggcgtgtggt cgggatggtc ggcctcgatc ccgtcgagcc
     3301 cctccccggc catgcgctcg atcagggagt ccgggaccgg gtcggcgtcg gaccacatcc
     3361 cggggtgggc gacaacgcag acgcctccgg cgcggtggat caggtacagc gcgtcgacgg
     3421 gatggagcgc gtgtttctcc acgtgcgccg cgcccccgtc ggcgatgaat gccgcggtga
     3481 acgcgtcctc gtagcgctcg acgagccccg cctccagcat ggcctgtgcg atgtgcgggc
     3541 gggccaccac gttccccgcc gccagctcgc gcacccgctc gtagccgatg ccgtggccca
     3601 tcgcgttcag ccgcttcacg atctcccggg cgcgccactc gcggtcgtcg cgcaggcggt
     3661 cgagctcggc ctggaactcg ccgttcgcgg cgtcgatcca gtaggccagg acgtggatgc
     3721 tgccaccctc gtagacggtc gagaactcgg tggccggcac cagctccagg ccggtgcccg
     3781 ccgcggcgtc ggcggcctcg tccaggccgg cggtggtgtc gtggtcggac agcgccagca
     3841 gagagacgcc gcgctcgagg gcgagccgga ccacctcggt tgggctgaac gtgccgtccg
     3901 atgccgtgct gtgcgtgtgc aggtcgatcg tggccacggg agcggccatg ctagcgaacg
     3961 ggcgccgtgg cgccggtccg ggtctcagcc gcgtcccggc cgagcgaacc ggccgcgcct
     4021 actgcggagc cgtccccacc aggatccggg tggagaagtg cgtctcgacc agggttccgc
     4081 cctggaacac gtaacagtca tcgcccgact cgtcgtagcg gaactgcacg aagagcatcc
     4141 ggaaggtgcc gcccttcagc acgaccgacg gagactgcag gtcgcagtcg cgctgcccgt
     4201 tcgtggtgaa gtcccagggc acgaccggga cgtcggaccc atgggtccac ccgccggccg
     4261 cggaggacga cgacgcgacg ccgaagccgt tgacggtggt gaacgggtcg ccggggtcgt
     4321 cgttctgcaa gcagcagccc acgtaccaga gccggtaccg gttggccgcc ggatcgaaca
     4381 gggccttcgg ctggaagagg ccgaagtccg cttcccacgg gatgcccttc cgtccggtga
     4441 gcaccggctc cggctggctg ttccaggaga ggccgtcggt ggaggcggcg tacccgatcg
     4501 ccccgcgccc gtcgttgttc gtcgccgcga accacatgcg gtacttgccg cccttgaaga
     4561 tcaccgagag ctgcagcggc ccgttcccgg agaactcgcc cgtgtcgccg gaccacaccg
     4621 gcgactgccg cgtggtccag tggaggccgt cctgcgagat ggcccggccg acgcgaaacc
     4681 ggcccgaggt gttcgtcgcg gtgaagtaca tccggaagcg gttttcggcg aacagcaccg
     4741 agatgtcgac gatcccgccg tcgtcccacg gcttgttgga gaagaggccc ggtagcggcg
     4801 cgccgctgcg cgtggtccaa tcggttccgc cctccgagga ggcggcgtac cccacgccgt
     4861 tcttgccgcc ctccttcggc acgctgacgt accagagccg gaagaggttt tccttgaaca
     4921 gcacgctcgt ggaagcgtgg aacggcctct cccaggtccc cttgatcggc ggactgggga
     4981 ccgtgcgcga gaacgggccg gcgggccccg cggacgccgg tgcggcgccc accgccgcga
     5041 gcagcagggc gacgacaacg aggaggatgg gtcgtctcat ggtcgttcct ctggtcgttt
     5101 ctcagccggg ggctccaccc gcatgctacc ccccgaaggc gctctctccg gtgagcgcgc
     5161 gaccgatgat cagcttctgg atctggctgg tcccctcgta cagcgtcgtc acccgggcat
     5221 cgcgcaggaa cttccccacg gggtactcgt cgacgtatcc gtagcccccg tgcacctgga
     5281 ccgccgcgtt cgcggctcgc accgccatct cgctggcgaa gaacttggcg taggacgact
     5341 ccagcgtgtg gccgcgcccg gcgtcggcga gcgccgccac ccgccaggtc agcaggcgcg
     5401 ccgcgtcgac ctcgacggcc atgtcggcga gcagctcctg taccagctgg aaccgggcga
     5461 tcggccggcc gaacgcccgt cgttccctcg cataggcgat gctcgcctcg aggcagccct
     5521 ggccgatccc cacgcagccg gccgcgagtg acatccgccc gctgtcgagc gccgacatcg
     5581 ccaccgagaa gccctgtccc tcgtcgccga ggcggttcgc gtcggggacc cggacgcggt
     5641 caaggaacag ctcggccgtg tcctgggcgc gcaggccgag cttgcctttc accggccgcg
     5701 aggagaatcc cggcacgtcg gtcgggacca ggaagcaggt gatgcccttt tgcccctcgc
     5761 cgccggtacg agcgaagacc agcgcgaccg cggcgtcgtt cccgttcgtg atgaactgct
     5821 tctgcccgct gatcagccag tcgccgccgt cccgttccgc ccgtgaggtc agggcggcgg
     5881 catccgaccc gacgcccggc tcggtcagcc cgaagcagcc gaggatctcc ccggcggcca
     5941 ggggggtcag ccacgcacgc ttctggtcct cggtcccgaa ccggtacagc gtcttcccga
     6001 ccagcccgac gttcaccgag accacgccgc gcaccgatga gtcgccgcgc cccagctcct
     6061 ccacgatcag gcagtacgac aggttgtcca ggcccatgcc gccgtacagc tcggggatca
     6121 ccgccccgag aaagcccagc tcacccagct gcctgacgag ctcgggatcg agccgctccg
     6181 cgcggtccga atcccgggcc cgcgggacga cttccttgtc gacccactcc cgggcggtct
     6241 tgcggatcag ctgctgctcg tcgctgagct ccaggtccat ggcccgatcc tatgcgaccg
     6301 gtcgagccag gcctccgccg gcggggcggc caccgcccgg gccgccggag tgaccggcgc
     6361 cacgccgccg atttggtcgt tcgggcggag gagtcgggcg gccgccgtcg aagtctcaga
     6421 gcggaagcga aaggagcccg cccatgagcg gcacgctggc ccgtgcacgc gccgcccaca
     6481 ccgatggcct cctggccgcg cccctggagc gggaaggggc ctggggagcc atccgatcgc
     6541 ttcgccaggc gatgcgccgg cttcgccaga ccggtctcga gccccgcgtc gagttcatcc
     6601 tcggcctcgg cgcccgcggc gagctcacct cgctctccgc ctccagcctc ctcctcctgg
     6661 gctacggtcc ggaggacgac gtacccgccc gacggacgcc acgtcgttcg cggccggcgc
     6721 cggtcctcag tggcggatca ggacgtcgat gtcgccctcc ttgcgaggga cctgagtggc
     6781 ggtgacgccg tcgtgggact ggaaggagat gtcgaggcgc gtgtcgccga cgcgcaggcc
     6841 gtgcacctcg atgcgctcga gccagcgcgg caggtgcggt cggatcaggt tcagcacccc
     6901 ctccggcgcg tcggcggtga tccccccgta cgtgcgcagc atcaggagcg gcgcgcccgc
     6961 cgaccaggcc tgcgggcggc aggccaccgg gaactgcacc ggcacgggaa cctcctcggc
     7021 ggagaacccg cagaacagct cggggtagcg cgcgtacggg aagaacatcc ccgccagcga
     7081 gagctggtcg atcacctgca tcgcctcgtg gtccagcccg tagcgcttca gcccgtgcgc
     7141 gatcagggcg ttgtcgtgcg gccagacggt ccccgtgtgg tagccgatcg ggtcgtacgg
     7201 cacctgccgc gcggcgagcg tgcggatccc ccagccggag gacagcccgg gcgagagcag
     7261 cttgcgggcc acgcgcgggg cc
//
LOCUS       DASPWO010000095         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_527396, whole genome shotgun sequence.
ACCESSION   DASPWO010000095 DASPWO010000000
VERSION     DASPWO010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527396"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VEO00_02170"
     CDS             complement(<1..213)
                     /locus_tag="VEO00_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56842.1"
                     /translation="MHDLRRDLPERRELVLGNGGGADPGVAAEAPKGGRTVRSELDAE
                     ERGVLGDTLEAVRPAAQRVLFRRLLRR"
     gene            579..953
                     /locus_tag="VEO00_02175"
     CDS             579..953
                     /locus_tag="VEO00_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56843.1"
                     /translation="MRDRIPAHGAARGRTDDRILVADGRRISPWRVSLGRLPDDVAMV
                     RRYRVTQRAATEFLVEVVWHERRSVGFAERLRDLYSEAVGALVRVEVRELDALPPRQA
                     AHDPLPGALMLADRWRWRRTAV"
     gene            1217..1498
                     /locus_tag="VEO00_02180"
     CDS             1217..1498
                     /locus_tag="VEO00_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56844.1"
                     /translation="MSPYLVMVGTLILEWATAIRRYHVVPRAPDDFLIEVVWRAERRQ
                     DLIERIPSAFASILGATVGVTVIDVDAMAFPLRGKARLVESRVGTAGAA"
     gene            1521..>2010
                     /locus_tag="VEO00_02185"
     CDS             1521..>2010
                     /locus_tag="VEO00_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56845.1"
                     /translation="MIEAALRVPVYRERWARIDAGSVRTVADLPLLPAVGRTELASRS
                     LEERLASGPDHPVLVVSTGTSGRSMESARSPEEDAFQQALWLRQERAQGLPLEAPHLH
                     LRIGLDRMGPLSVDGPRATLGAYRPIGEQVAAARRLEPRVLIGPPSVVVELGEALGGR
                     PVD"
BASE COUNT          229 a          717 c          781 g          283 t
ORIGIN      
        1 acggcgcagg aggcggcgga acaggacgcg ctgcgcggcc gggcgtaccg cttccagggt
       61 gtccccgagg acgccccgtt cctcggcgtc gagctcggac cgaaccgtac ggcccccctt
      121 cggcgcttcg gccgcgacgc ccggatcggc gccgccgccg ttgccgagga ccagctcgcg
      181 gcgttccggg agatcgcgcc gcaggtcgtg cacgggccgc cgagcatcct ggccgacctg
      241 gccgagcgga tgagcccgcc gtcgttgcgc gccctgctga ccgcgtccga gcagctcggg
      301 cccgagctcc ggcgggagct gcgggatgcc tacggggcgg acccgctcga caactacgga
      361 gcgaccgagg tgggagccgt ctcgtggcag tgcgagcgac gcgacggcta ccacgtcaac
      421 gccgatgcgg tcgtcgtgga ggtggtcggc gaggacggcc tgccggtccc ggcgggcgtc
      481 accggcgagc tggtgatcac ggcgctgtgg aacacgtcgg cgcccgtcgt ccgacaccgc
      541 gtcggggacc tcggcgcgct cgtcggcggc cggtgcccat gcgggatcgg attcccgctc
      601 atggcgcagc ccgggggcgc accgacgacc gcatcctggt ggccgacggg cggcggatct
      661 cgccgtggcg agtgtcgttg ggacgcctgc ccgacgacgt cgccatggtc aggcgctacc
      721 gggtcacgca gcgggcggcg accgagttcc tggtcgaggt cgtctggcac gagcgacgct
      781 ccgttgggtt cgccgagcgc ctgcgcgacc tctactctga ggcggtcggg gccctcgtgc
      841 gcgtggaggt gcgagaactg gacgcgttac cgccccggca agccgcgcat gatccgctcc
      901 ctggcgcgct gatgctcgcc gatcggtggc ggtggcggcg taccgccgtg tgaatcggat
      961 gctccgcgcc gggcccgagg agcttcgggc gctccaggat gctcggctcc gcgcggtcct
     1021 ggtcgcggcg cggacggccg agccgtacct gaccttctgg cggggcctgc ccggcgacct
     1081 cgtccgctcg tcgcgcgacc tatggcgcgg ctgccctcac gacgcgcgac ggcctgcggg
     1141 tgtggggtga cgctgcccag catcggccca tgagggccgg tcgatcgacg ccgtcgtcac
     1201 acccgacggc cgccgcatgt cgccgtacct ggtgatggtg ggaacgctga tcctcgagtg
     1261 ggccacggcg atccggcgct accacgtcgt gccgcgcgcc cccgacgatt tcctgatcga
     1321 ggtcgtctgg cgcgccgagc gccgccagga cctgatcgag cgcatcccgt cggccttcgc
     1381 gagcatcctc ggcgctacgg tgggcgtgac cgtgatcgac gtcgatgcga tggccttccc
     1441 actgcgtggc aaggcccgcc tggtggaatc gcgggtcggg acggcgggcg cggcatgatt
     1501 cggcgggacg ggccggcgcc gtgatcgagg ccgcgctgcg cgtgcccgtg taccgggaac
     1561 ggtgggcccg gatcgacgcc gggagcgtcc ggacggtggc ggaccttccg ctgctgccgg
     1621 cggtggggcg aaccgagctg gccagccgct cgctggagga gcggctggcg tccgggccgg
     1681 accatcccgt cctggtcgtc agcacgggaa cgtccggacg ctcgatggag tccgcccgct
     1741 ccccggagga ggacgcgttc caacaggcgc tgtggctgcg gcaggagcgc gcgcagggtc
     1801 tcccgctcga ggcgccgcac ctgcacctgc ggatcggcct cgaccggatg ggcccgctct
     1861 ccgtcgatgg gccccgcgcc accctcggtg cgtaccgccc gatcggcgag caggtcgcgg
     1921 cggcgcgccg gctggagccg cgggtcctga tcggtccgcc gagcgtggtg gtcgagctgg
     1981 gcgaggccct cggtggtcgc ccggtcgacg
//
LOCUS       DASPWO010000096         3480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_527657, whole genome shotgun sequence.
ACCESSION   DASPWO010000096 DASPWO010000000
VERSION     DASPWO010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527657"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1926
                     /locus_tag="VEO00_02190"
     CDS             <1..1926
                     /locus_tag="VEO00_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HYU56846.1"
                     /translation="LTFVAPDPGNVTVFPGLSREWPSGTPVSVIRSMAPIAGRQATVL
                     ALPTTTGATDLFVTDGNAFAASEMVRLTSGEGVTFHTLLANGTPLTGGAANAADRPLM
                     VTLQSALVRAHPAGSAVVGRDPLIDVQALDAGIWGNRLRISVEDEAPGLVSTTLATIV
                     NATTVRLASASGVQPGTVLELFDASGSVGDPVKVSNVNRTAGFTLTLAGAGLAPLQQV
                     AGLGVRSREFRITVRLLRQPDPRTPSRDEEVIDFEVFRYLSLDPRHSNYVEKVIGAID
                     GPLRRWDRRPEGSSLYLRVSDRAANQAEEESVRVGPEALIDVLPSGLRRPARMRLEQV
                     VGSDSIGTLTDDHYVGADNADPDLRTGLQALRNIEEISIVGMPGRVGPRVQQALVDHC
                     ELLRYRFAVLDSKPEPTDTIADTQDQRGQFDTKYAALYYPWLSIPEPFPENLTIVRDY
                     AIPPSGHVLGVYARTDIDRGVHKAPANEVVRGIVGLRRKINKEQQDILNPYPVNINVI
                     RDFRDNNRGIRVFGGRVISSDPDWKYVNVRRLLIFIEASIERGLQWVVFEPNAEPLWA
                     RVRRVITNFLTVVWRNGALEGIKPEEAFFVKCDRTTMTQAEIDNGQLIVLVGVAPVKP
                     AEFVIVRIGLWTARSES"
     gene            1937..2377
                     /locus_tag="VEO00_02195"
     CDS             1937..2377
                     /locus_tag="VEO00_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HYU56847.1"
                     /translation="MSDTRVDPFRAFNFRLEIDGIAVAAFSEASGLTSTGEAVNYRTG
                     VDIPLTPRKLIGQRTFGPITLKRGMVQDDTLWDWYRNIATGSNDRRNGSVVLQDELHT
                     DVLRWNFENAWPNKIEGPTLNASGNEVAVESVELVVEDIILEVA"
     gene            2374..>3480
                     /locus_tag="VEO00_02200"
     CDS             2374..>3480
                     /locus_tag="VEO00_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56848.1"
                     /translation="MTAVHALLAPGVYYQTVDAGAPPVTPLRTDIAGFVGIAERGPID
                     LPVPVESWRQFASWFGGFSGAGYLAYAVRAFLENGGRRCWVVRVASRDAAAGAVASSS
                     IVDNVGGPVWRIRASSEGTWGDGLAITMRERNPGQVIATGTDPDGSFTAVPTTSGFDR
                     GTLVRVTQAGATAPRWKVVSHVDPHLGRLYWRHPDPALRMPYDGPLVGLDPGSPIVIR
                     SVEYALALYEEGRLIRFYDGLSIVARHERYGPARLFPPRPTFDPATGAFVSRPPEPLV
                     IDELRTDLTDVRGLDVDPSRMLRMTGGRDGLGELAVQDFVGEPVAPDDGPAAVADKRR
                     GLRALEEISEVALLAVPDAQVRPIEVHPVLPPEPC"
BASE COUNT          502 a         1276 c         1204 g          498 t
ORIGIN      
        1 ctgacgttcg tcgcgccgga ccccgggaac gtcaccgtgt tcccggggtt gtcccgcgag
       61 tggccgagcg gaacccccgt ctcggtgatc cgctcgatgg ctcccatcgc gggacgtcag
      121 gcgaccgtgc tggccctccc cacgacgacg ggcgcaaccg acctcttcgt gaccgacggg
      181 aacgcgttcg ccgcaagcga gatggtccgc ctgacgtcgg gggagggcgt gaccttccac
      241 acactcctcg cgaacgggac cccgctcacc ggtggggccg ccaacgccgc ggatcgcccg
      301 ctaatggtga cgctgcagag cgcgctcgtt cgggcccacc cggcgggctc ggcggtcgtg
      361 gggcgggatc cgctcatcga cgtgcaggcc ctcgacgcgg gcatctgggg caaccgcctc
      421 cgcatctccg tcgaggacga ggcgcccggc ctcgtcagca ccacgctcgc cacgatcgtc
      481 aacgccacga ccgtccggct ggcgtcggca tccggcgtcc agcccgggac ggtcctggag
      541 ctgttcgacg cgagcgggtc cgtcggcgac ccggtcaagg tgtcgaacgt gaaccgcacg
      601 gccgggttca cgctcaccct tgccggcgcg gggctggcgc cgctgcagca ggtcgccggc
      661 ctcggcgtcc ggtcccggga gttccggatc accgtccgac tgctgcgcca gccggacccc
      721 cggactccgt cccgtgacga ggaggtgatc gacttcgagg tcttccggta cctgtcgctc
      781 gacccgcgcc acagcaacta cgtcgagaag gtcatcggcg cgatcgacgg gccgctccgc
      841 cggtgggacc ggcgccccga ggggtcgtcg ctctacctcc gcgtctcgga ccgggcggcg
      901 aaccaggccg aggaggaatc ggtccgggtg ggccccgagg cgctcatcga cgtcctgccc
      961 agcgggctgc gccggccggc gcgcatgcgg ctcgagcagg tcgtcggctc cgactcgatc
     1021 ggcacgctga ccgacgacca ttacgttggg gccgacaacg ccgaccccga cctccgcacg
     1081 gggctccagg cgctgcgcaa catcgaggag atcagcatcg tcggcatgcc gggccgggtc
     1141 ggcccccggg tccagcaggc cctggtcgac cactgcgagc tgctccggta ccgcttcgcg
     1201 gtcctcgact ccaagcccga gcccaccgac accatcgccg acacccagga ccagcgcggc
     1261 cagttcgaca cgaagtacgc cgcgctctac tacccctggc tctccatccc ggagccgttc
     1321 ccggagaacc ttacgatcgt ccgcgactac gcgatcccgc cctcgggcca cgtcctgggc
     1381 gtgtacgcgc gcaccgacat cgaccgcggc gtgcacaagg ccccggccaa cgaggtcgtg
     1441 cgggggatcg tcgggctgcg ccggaagatc aacaaggaac agcaggacat cctcaatccg
     1501 tatccggtca acatcaacgt gatccgcgac ttccgcgaca acaatcgcgg tatccgggtc
     1561 ttcggcggcc gggtcatctc gagcgatccg gactggaagt acgtgaacgt ccggcgcctc
     1621 ctgatcttca tcgaggcctc catcgagcgc ggcctgcagt gggtcgtgtt cgagccgaac
     1681 gccgagccgc tgtgggcgcg cgtgcgccgt gtgatcacca acttcctgac cgtggtgtgg
     1741 cgcaacggcg cgctcgaggg catcaagccc gaggaggcct tcttcgtcaa gtgcgaccgc
     1801 acgacgatga cgcaggccga gatcgacaac gggcagctca tcgtgctcgt cggcgtggcg
     1861 ccggtgaagc ccgccgagtt cgtcatcgtc cgcatcggtc tgtggacggc tcgatcggag
     1921 tcctaggagg ttcgccgtgt cagacacccg ggtcgaccca tttcgcgcct tcaacttccg
     1981 gctcgagatc gacggcatcg cggtggccgc gttcagcgag gccagcggcc tgacctcgac
     2041 cggcgaggcc gtcaactacc gcaccggcgt cgacatcccg ctcacgccgc gcaagctcat
     2101 cggccagcgc acgttcggcc cgatcacgtt gaagcgcggc atggtccagg acgacacgct
     2161 gtgggactgg taccgcaaca tcgccaccgg ctcgaacgac cgccggaacg gcagcgtggt
     2221 cctccaggac gagctgcaca ccgacgtgct gcggtggaac ttcgagaacg cgtggcccaa
     2281 caagatcgaa ggtccgacgc tcaacgcgag cggcaacgag gtcgcggtgg agagcgtcga
     2341 gctcgtcgtc gaggacatca tcctggaggt ggcgtgacgg ccgtgcacgc cctcctggcc
     2401 cccggcgtct actaccagac cgtcgacgcg ggcgcgcccc cggtcacccc gctgcgcacc
     2461 gacatcgccg gcttcgtcgg catcgccgag cggggaccga tcgacctgcc ggtcccggtc
     2521 gagtcctggc ggcagttcgc atcgtggttc ggcgggttct ccggggccgg gtacctcgcg
     2581 tacgccgtcc gggcgttcct cgagaacgga ggccggcggt gctgggtggt gcgcgtggcg
     2641 tcgagggacg ccgcggcggg cgcggtcgcc tcctcgtcga tcgtggacaa cgtgggaggc
     2701 cccgtctggc gcatccgcgc atcgagcgag ggcacgtggg gcgacgggct cgcgatcacc
     2761 atgcgcgagc ggaaccccgg acaggtcatc gcgaccggga cggaccccga cggcagcttc
     2821 acggccgtgc cgacgacgag cggattcgac cggggcacgc tcgtgcgggt cacccaggcc
     2881 ggggcgacgg ccccccggtg gaaggtcgtg tcgcacgtgg acccccacct ggggcggctg
     2941 tactggcgcc atccggaccc cgcgctccgg atgccgtacg acggcccgct ggtggggttg
     3001 gaccccggct cgccgatcgt gatccgcagc gtcgagtacg cgctggcgct gtacgaggag
     3061 ggccgcctga tccggttcta cgacgggttg tcgatcgtcg cccggcacga gcggtacgga
     3121 ccagcacggc tgttcccgcc gcggcccacg ttcgaccccg ccaccggcgc gttcgtgtcc
     3181 cggccgcccg agcccctcgt gatcgatgag ctccggaccg atctcaccga cgtccggggc
     3241 ctggacgtcg atccctcccg gatgctgcgc atgaccgggg gccgcgacgg cctcggcgag
     3301 ctcgccgtgc aggacttcgt cggcgagccc gtggcgcccg acgacgggcc ggccgccgtc
     3361 gccgacaagc ggcgcggcct gcgggccctg gaggagatct cggaggtcgc gttgctcgcc
     3421 gtgcccgacg cccaggtgcg cccgatcgag gtgcaccccg tcctcccgcc ggagccctgc
//
LOCUS       DASPWO010000097         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_865742, whole genome shotgun sequence.
ACCESSION   DASPWO010000097 DASPWO010000000
VERSION     DASPWO010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865742"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1861)
                     /locus_tag="VEO00_02205"
     CDS             complement(197..1861)
                     /locus_tag="VEO00_02205"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HYU56849.1"
                     /translation="MRIALGQINTTVGDLEGNVERMVRSTRDATAAGADVIVFPELGV
                     TGYPPEDLLLRRAFVDDNLEALDDLARRTSGACAALVGFVDRTERGLHNAAAVVRGGR
                     VLERYHKHRLPNYGVFDEARYFVPGIAACALRLADSSLGISICEDAWAPGPPFDVYAG
                     SVRVIPNLNASPYHRGKTAERLEVCRARARETGAWIVYVNAVGGQDELVFDGGSIVVA
                     PDGELAWHARSFEEDLLLVDLLGPGEDKPPLPDPHRPAWPDPDEEVYRALLVGLGDYV
                     HKNGFDRAYVGMSGGIDSTMVAVLAADALGPGAVRGLAMPSGYSSEGSVSDAEAVAER
                     LGIRLDRVPIQPIVDAYLETVRPLFGGRPADVSEENLQARIRGNLLMAMSNKFGGLVL
                     ATGNKSEMATGYSTLYGDLAGGFAPIKDVMKTLVYRVARWRNARAERDGDTPPIPESV
                     LSKAPSAELRPGQLDTDSLPPYEELDPILEEYIEEDRSPEEIVAVGYDHELVDRVVRM
                     VDLSEYKRRQAPPGVKITPKAFGRDRRLPITNRYRRTPAPAEPSQP"
     gene            complement(1902..>2135)
                     /locus_tag="VEO00_02210"
     CDS             complement(1902..>2135)
                     /locus_tag="VEO00_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HYU56850.1"
                     /translation="ASNNIYEMSDDERRAAGIEALPEDLYDAIKVAERSELLREALGE
                     HVVDYLLRNKKEEWDGYKAYVTPFELERYLPLL"
BASE COUNT          300 a          775 c          740 g          320 t
ORIGIN      
        1 cggcatcgag atgaccgggc cgggctcccc gctcggctcc tcggacgaca acgagatccg
       61 cgacaactcc tcgaaggggc acacgtacga cctgcgcaac ctgggcagcg gcaactgctt
      121 cgcgaacaac ctctacgtca ccagccaagg gccgctcccg ccctgctgag ccgcagccgc
      181 gctgcgcctc ccgaacctag ggctgcgacg gctccgcggg agcgggcgtg cgccggtagc
      241 ggttggtgat cggcaggcgc cggtcgcgcc cgaaggcttt cggcgtgatc ttcacccccg
      301 gcggggcctg gcggcgcttg tactcggaca ggtccaccat ccgcaccacg cggtcgacga
      361 gctcgtggtc gtaccccacc gccacgatct cctcgggtga gcggtcctcc tcgatgtact
      421 cctcgaggat cgggtcgagc tcctcgtacg gcgggagcga atctgtgtcg agctggccgg
      481 gtcggagctc cgcggagggc gccttcgaga gcacgctctc cgggatgggc ggcgtgtcgc
      541 cgtcacgctc ggcccgggcg tttcgccagc gcgccacccg gtagacgagc gtcttcatca
      601 cgtccttgat cggggcgaag ccgccggcca ggtccccgta gagcgtcgag tacccggtgg
      661 ccatctcgct cttgttgccg gtggcgagaa ccagcccgcc gaacttgttg gacatggcca
      721 tcagcaggtt cccgcggatg cgcgcctgca ggttctcctc ggacacgtcg gcgggacggc
      781 ccccgaacag gggacggacc gtctccagat acgcgtcgac gatcggctgg atcgggacgc
      841 ggtccaggcg gatcccgagg cgctcggcga ccgcctccgc gtccgagacg ctgccttcgg
      901 aggagtaccc ggacggcatg gccagcccgc gcaccgcccc cggcccgagg gcgtcggccg
      961 ccagcacggc caccatcgtc gagtcgatcc cccccgacat gccgacgtag gcccggtcga
     1021 acccgttctt gtgcacgtag tcgccgagac cgaccaggag cgcgcggtag acctcctcgt
     1081 cggggtccgg ccaggccggg cggtgtgggt cggggagggg cggcttgtcc tcgcccggcc
     1141 cgaggaggtc gaccagcagc aggtcctcct cgaacgagcg cgcgtgccag gccagctcgc
     1201 cgtcgggggc gaccacgatg ctgccgccgt cgaagaccag ttcgtcctgg cctccgaccg
     1261 cgttgacgta cacgatccac gcgccggtct cgcgggcccg ggcgcggcac acctcgagcc
     1321 gctcggcggt cttcccccga tggtacggcg acgcgttcag gttcgggatg acgcggacgc
     1381 tgccggcgta gacgtcgaag ggcgggcccg gcgcccaggc gtcctcgcag atcgagatgc
     1441 cgagcgagga gtcggccagg cggagcgcgc atgccgcgat ccccggcacg aagtagcgcg
     1501 cctcgtcgaa cacgccgtag ttcggcagcc tgtgcttgtg gtagcgctcc aggacccggc
     1561 cgccgcggac gacggcggcg gcgttgtgca ggccccgctc ggtgcggtcg acgaacccga
     1621 cgagcgctgc gcaagcgcct gaggtccgtc gggccaggtc gtcgagggcc tcgaggttgt
     1681 cgtcgacgaa ggcccgccgc agcagcaggt cctccggcgg atatccggtc acgccgagct
     1741 cggggaagac gatcacgtcc gccccggccg ccgtggcgtc gcgggtggac cgaaccatcc
     1801 gctcgacgtt gccctcgagg tcaccgaccg tggtgttgat ctgcccgagc gcgatcctca
     1861 caccgtcacc gtacggcttc cccccggact cgtccggcgc ttcagagcag cgggaggtag
     1921 cgctccagct cgaagggcgt cacgtacgcc ttgtacccgt cccactcctc tttcttgttg
     1981 cgcaggaggt agtcgacgac gtgctcccca agcgcctcgc gcaacagctc gctccgctcg
     2041 gcgaccttga tcgcgtcgta caggtcctcg gggagcgcct cgatcccggc cgcccgacgc
     2101 tcgtcgtcgg acatctcgta gatgttgttc gaggc
//
LOCUS       DASPWO010000098         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_187895, whole genome shotgun sequence.
ACCESSION   DASPWO010000098 DASPWO010000000
VERSION     DASPWO010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187895"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..921)
                     /locus_tag="VEO00_02215"
     CDS             complement(208..921)
                     /locus_tag="VEO00_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HYU56851.1"
                     /translation="MAATTVRTLRIGGHALRFVVSERREEERLGALATEEGRMLRRLL
                     AAIQEGDTFFDVGANIGTLTLPVAVAGGVECLAFEPEPANAARLAENAELNGLTNVTV
                     IEAAVWGVPGTVGLRVDGAAGTGTGRVVADRGDGVAQVPAVTVDAFAGGGSAAPQVLK
                     LDVEGAELEVLRGAAGALASGRIRDLFVETHPGELAERGKSEAELDALLGAHGYAEVW
                     AARRGAETHRHFRRAALAG"
     gene            957..1697
                     /locus_tag="VEO00_02220"
     CDS             957..1697
                     /locus_tag="VEO00_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HYU56852.1"
                     /translation="MAAARDTLQVASRDAFGSRESRRLRRGGFVLGVVYGGGRDARPF
                     QVGEREIATFLSNGQTLFDLKIDGSAAVPVVVKEQQHHPVRGQLLHIDCLEVKLDEAI
                     QAEVAIEVQGSEEAPGVTEGGVLEHVTREVTIEALPEDIPEVIYADVSEMVIGDTLQL
                     AAVPAPSGVTYVADDLEEVTVATLSPPRVEEEPEPELEEEAELVGEEGEVPEGEEVPE
                     GEAPAEGEAPEGGGAEGGGEGGEAPGGE"
     gene            1761..2309
                     /gene="pth"
                     /locus_tag="VEO00_02225"
     CDS             1761..2309
                     /gene="pth"
                     /locus_tag="VEO00_02225"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HYU56853.1"
                     /translation="MGLGNPGPRYDRTRHNVGFVTLGELVRRWELPRARDRFRGRIAE
                     GRAGAGGPRVAALQPLTFMNESGTSVGPARGSLKVPLERLVVLHDEIDLPFGEVQARL
                     GGGLAGHNGLRSVAAGVGGPDFWRVRIGVGRPDSTDPDVVSAYVLGRFAESEGEVRDL
                     VAAAANEAERLVRSLSQSEAAA"
BASE COUNT          311 a          885 c          884 g          306 t
ORIGIN      
        1 cgcggtcatt gcatcagcga ccgtcccggg ttgcggggag gcccggaagg acccggccga
       61 ggtcatttcc gagcagtccc ggaaggatct tccggccctc cccgccgtga caatcgagtg
      121 ccctgacggc tcagccgagt caaccaagga ggagtgccga tggtcggagg gagctatcgc
      181 cgccgtcggg gccgctcaga cgggtcgcta gccggcgagc gccgcgcgcc gaaagtggcg
      241 gtgcgtctcg gcgccgcgcc gcgccgccca cacctcggcg tagccgtgcg cccccagcag
      301 cgcatccagc tccgcctcgc tcttcccgcg ctccgccagc tcgccggggt gggtctcgac
      361 gaacaggtcg cggatgcgcc cgctcgccag cgcgccggcg gcgccgcgaa ggacctcgag
      421 ctcggcgccc tcgacgtcga gcttgagcac ctggggcgcg gcgctcccgc cgccggcgaa
      481 cgcgtccacc gtgaccgccg ggacctgggc gacgccatcg ccccgatcgg cgaccaccct
      541 tccggtaccc gtccccgccg cgccgtcgac gcgcagcccg accgtcccgg ggacccccca
      601 cacggccgcc tcgatcaccg ttacgttggt gaggccgttt agctcggcgt tctcggcaag
      661 gcgcgcggcg ttggccggct cgggctcgaa ggccaggcac tcgacccccc cggccaccgc
      721 cacgggcagg gtcagcgtcc cgatgttcgc cccgacgtcg aagaacgtgt cgccctcctg
      781 gatcgcagcc agcaggcggc gcagcatccg cccctcctcg gtggcgagcg cgccgaggcg
      841 ctcctcctcg cggcgctccg acacgacgaa gcgcagcgcg tggcccccga tccgcagcgt
      901 tcgcaccgtc gtggcggcca tggcgttcag tatgggacag ctatcctcac cggtccatgg
      961 cagccgctcg agacacgctc caggtcgcct cgcgcgacgc cttcggctcg cgtgagagcc
     1021 gccgtcttcg ccgcggcggg ttcgtgctcg gcgtcgtcta cggcggcggc cgcgacgccc
     1081 gcccgttcca ggtcggcgag cgcgagatcg cgaccttcct gtcgaacggc cagaccctgt
     1141 tcgacctgaa gatcgacggg tccgcagccg tccccgtcgt cgtcaaggag cagcagcacc
     1201 acccggtgcg cggccagctc ctgcacatcg actgcctcga ggtcaagctc gacgaggcga
     1261 tccaggccga ggtcgcgatc gaggtccagg gctccgagga ggcgcccggc gtcacggagg
     1321 gcggcgtgct cgagcacgtc acccgcgagg tcacgatcga ggcgctgccg gaggacatcc
     1381 cggaggtcat ctacgccgac gtctccgaga tggtcatcgg cgacacgctt cagctcgccg
     1441 cggtcccggc gcccagcggg gtcacctacg tcgccgacga cctcgaggag gtgaccgtcg
     1501 cgaccctctc tccgcctcgc gtcgaggagg agcccgagcc cgagctcgag gaggaggccg
     1561 agctggtcgg cgaggagggc gaggtgcccg agggcgagga ggttcccgag ggcgaggccc
     1621 ctgcggaggg cgaggccccc gagggcggcg gcgccgaggg cggtggcgag ggcggcgaag
     1681 cccccggtgg ggagtagccg ctgagcctca tcgaccggct gcgcggaagg caggccggcg
     1741 gagaggagcg gatcctggtc gtgggcctcg gcaacccggg gccgcgctac gaccgcacca
     1801 ggcacaacgt cggcttcgtg acgctgggag agcttgttcg ccgctgggag ctgccgcggg
     1861 cccgcgaccg ctttcgcggt cgcatcgccg aggggcgggc gggggccggc ggcccgcggg
     1921 tggcggcgct ccagccgctg accttcatga acgagtcggg gacgtcggtc ggtcccgcgc
     1981 ggggatcgct caaggtcccg ctggagcggc tcgtggtcct gcacgacgag atcgacctgc
     2041 cgttcggcga ggtccaggcc cgcctcggtg gcgggctggc cggccacaac gggctgcgca
     2101 gcgtcgccgc cggcgtcggc ggcccggact tctggcgcgt gcggatcggc gtcgggcgcc
     2161 ccgactcgac cgatcccgac gtggtctcgg cctacgtgct gggccgcttc gccgagtccg
     2221 agggtgaggt gcgcgatctg gtcgccgccg cggccaacga ggccgagcgg ctggtccggt
     2281 ccctgtcgca gagcgaggcc gcggcatgag cgagctgacg agctacgacg tcgacgaggc
     2341 cggcgtcgcc ctgctgcgcc tcgaccgtcc gaaggcgcgc aacgcg
//
LOCUS       DASPWO010000099         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1038609, whole genome shotgun sequence.
ACCESSION   DASPWO010000099 DASPWO010000000
VERSION     DASPWO010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038609"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VEO00_02230"
     CDS             <1..1011
                     /locus_tag="VEO00_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU56854.1"
                     /translation="QMASLFAATYPERTRALVTYGSYARLRSAPDFPWGQSDEFLENF
                     ATNMEATWGGEIPEGSVWAVNATRDPQFRDWLGRWMRNAASPGAADTLLRMAYEIDVR
                     PVLPAIRVPTLVLHRVDDPLIPVDQGRYFAAQIPGAKFVELPGNDRLVFAGDVDRLVD
                     EIEEFLTGQRHQISPNRILATVLYTDIVDSTRRASELGDQRWRELLDRHDELMRHEFE
                     RFRGEEMTATGDGFLVAFDGPARAVHCALAAANTARSLGLELRVGVHTGECEQRGDSL
                     SGIAVHIAARVGALAQPGQVLVSRTVTDLVAGSRLEFTDRGEHDLKGVPGQWHLLAAQ
                     AA"
     gene            1114..2133
                     /locus_tag="VEO00_02235"
     CDS             1114..2133
                     /locus_tag="VEO00_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56855.1"
                     /translation="MRLVSRIPALTAAAALAGGLAVATGGSAGGQSTESQTFTFTGAD
                     QTFTVPDGVTEITVDASGAQGGAAADPPSGGSGSGGPGGNGSQSVATIAVTPGEQLIV
                     IVGGQGGTSTGTSGGAGGFGAANERFEGGGGGSISDEDDASSGGGGGGGSAALRGTTA
                     LVVGAGGGGGGGAGFQSDSLGPPDGGGGGTGDGDGADGTPPCEGAGGTSGNAGGAGGA
                     GGTVSCVGGAVAGTDGQNGFADGPGGAGGAGATGGGADAGGGGGGGGGIAGGGGGGGG
                     AGSPTLFGTGGGGGGGASLAVTTQPGVRAGNGVVVISWTTGAAPAPGAPAPAAPVVGT
                     PRFTG"
     gene            complement(2149..2538)
                     /locus_tag="VEO00_02240"
     CDS             complement(2149..2538)
                     /locus_tag="VEO00_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HYU56856.1"
                     /translation="MILVDTSAWVEFLRDTGTSACNRVDALLDADIATCHPIRMEVLA
                     GARDEGHLSDLRRLLARAALVPTEPIDYEEGAALYRACRRAGETPRRLIDCLIAATAI
                     RAHAAVLHADADFDVVARHTALRLDSP"
     gene            complement(2535..2738)
                     /locus_tag="VEO00_02245"
     CDS             complement(2535..2738)
                     /locus_tag="VEO00_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HYU56857.1"
                     /translation="MSRTNIDLDDNACRAVMERYHLATKRDAVNFALRTVAAEPLSLD
                     QARRLRGSGWDADLDEMRASRAG"
     gene            complement(2790..>3269)
                     /locus_tag="VEO00_02250"
     CDS             complement(2790..>3269)
                     /locus_tag="VEO00_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262476.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HYU56858.1"
                     /translation="AKPPAWVLGTAMRSEPTMFAERDAVNPQAGKDCAADVFGQAGIT
                     TPRDQIDCIEMYVPFSWYEPMWLENLGFVPEGDGWKMTEKGATALDGELPVNMSGGVL
                     SSNPIGASGMLRFAEAALQVRGQAGEHQVDNARTALGHAYGGGSQFFAMWVVAAEKP"
BASE COUNT          468 a         1140 c         1199 g          462 t
ORIGIN      
        1 cagatggcgt cgctgttcgc tgccacctat cccgagcgca ctcgggcctt ggtcacgtac
       61 gggtcctacg cccggctccg aagcgccccc gatttcccgt ggggccagtc cgacgagttc
      121 ctggagaact tcgcgacgaa catggaagcc acgtggggcg gcgagatccc cgaggggtcc
      181 gtgtgggccg tcaacgcgac gcgcgatccg caattcaggg actggctcgg ccgctggatg
      241 cggaacgcgg cgagccccgg agccgctgac accctgttgc gcatggccta tgagatcgac
      301 gtgcgcccgg tgctgccggc catccgggtc ccgaccctcg tgctgcaccg cgtcgacgat
      361 ccgctcattc cggtcgacca gggccgttac ttcgccgcgc agatcccggg ggccaagttc
      421 gtcgagcttc ccggcaatga ccgtctcgtc ttcgccgggg atgtggaccg cctcgtcgac
      481 gagatcgagg agttcctcac cgggcaacga caccagatca gtcccaaccg gattctcgcc
      541 acggtcttgt acaccgacat cgtcgactcg acgcgacggg catccgagct cggtgaccag
      601 cgctggcgcg agctgctcga ccgccacgac gagctcatgc gccacgagtt cgaacggttc
      661 cgcggcgagg aaatgacagc aaccggcgac ggattcctcg tcgcgttcga tggtcctgct
      721 cgtgcggtgc actgcgccct ggccgcggcg aacacggcgc gcagtctcgg tctcgaatta
      781 cgtgtcggcg tccacaccgg tgaatgcgaa cagcgcggcg acagtctcag cggcatcgca
      841 gtgcacatcg cggcccgcgt cggtgcgctc gcgcaaccgg gccaagtgct cgtctcgcgc
      901 actgtcaccg atctcgtggc cggatcccgc ctggaattca ctgaccgcgg cgaacacgat
      961 ctcaagggcg ttccggggca gtggcacctc ctcgccgccc aagcggccta gtcacccaac
     1021 cactcccgac ctgatccgac cggagtcact agggtgtgac ccgactttgg gccgagcccc
     1081 agatcttgat acgtcgcgtc gaagggtggt cggatgcgac tcgtcagtag gatcccggca
     1141 ctcaccgctg cggccgcgct cgccggcggc ctcgccgtcg ccaccggagg atcagcgggt
     1201 ggccagtcga cggagtcgca gaccttcacg ttcaccggcg ccgaccagac gttcacggtt
     1261 cccgacggcg tgacggagat caccgtcgac gcgtccggcg cccagggagg cgcggcggcc
     1321 gatccgccga gtggcggcag cggcagcggc ggacccggcg gcaatgggag tcaatcggtc
     1381 gccacgatcg cggtcacacc tggtgagcag ctgatcgtga tcgtcggcgg tcagggcggc
     1441 acgagcaccg gcaccagcgg gggcgccggc ggcttcggcg ccgcgaacga gcgcttcgag
     1501 ggcggtggcg ggggcagcat ctccgacgag gacgacgcct ccagcggcgg cgggggtggc
     1561 ggcgggtcgg ccgcattgcg tgggaccacc gcgctcgtcg tcggcgccgg cggtggcggt
     1621 ggcggcggcg ccggcttcca aagcgacagc ctcgggcctc ccgatggcgg cggaggcggg
     1681 acgggtgacg gcgacggcgc cgacgggacg ccgccgtgtg agggcgcggg cggcacgtcc
     1741 ggcaacgcgg ggggcgccgg cggggccggc gggactgtct cgtgcgtggg tggcgcggtc
     1801 gccggcaccg acggtcagaa cggcttcgcg gacggtcccg gcggcgctgg cggcgccggc
     1861 gcgaccgggg gaggcgccga cgccggcggg ggcggcggcg gcggaggcgg catcgccggc
     1921 ggtggcggcg gaggcggtgg cgccggcagc cccaccctct tcggcaccgg cggcggcgga
     1981 ggcggcggcg cgagccttgc ggtcaccacg cagcccggag tccgcgccgg gaacggcgtc
     2041 gtcgtgatct cctggacgac gggcgcggct ccggctcccg gggcccctgc tcccgcggcg
     2101 ccggtggtgg gcacaccgcg cttcaccggc tgacggcgca cgcgccggct acggcgaatc
     2161 gagcctcaga gcggtgtggc gggcgaccac atcgaagtcg gcgtcggcgt gcaagacggc
     2221 ggcgtgggct cggatagcgg tcgcggcgat gaggcagtcg atgagtcgtc gcggagtctc
     2281 gcccgctcgg cggcacgctc ggtagagcgc agcgccctcc tcgtaatcga tcggctcggt
     2341 cgggaccagc gcggcgcgag cgaggaggcg tcgaaggtcg ctcagatggc cctcgtcacg
     2401 agcgccggcg agcacctcca tacgaatcgg atggcacgtg gcgatgtcgg cgtccaggag
     2461 cgcatcgacg cgattgcaag cggaggtgcc tgtgtcacgc aggaactcga cccacgccga
     2521 ggtgtcgacc aggatcaccc ggcgcggctc gctcgcatct catcgagatc ggcatcccac
     2581 ccagagccac gcagacgccg tgcctggtcg agactcagcg gctcggccgc gaccgtccgg
     2641 agggcgaagt tgacggcatc gcgcttggtg gcgaggtgat agcgctccat cacggcgcgg
     2701 caggcgttgt cgtccaagtc gatgttggtg cgtgacatac acctaatata caccacggcg
     2761 ttccgcggaa cgccgggtgc gtcagccctt tacggcttct ccgccgcgac gacccacatc
     2821 gcgaagaact gggagccgcc gccgtaggcg tggccgaggg cggtgcgggc gttgtcgacc
     2881 tggtgctccc ccgcctggcc ccgcacctgc agcgcggcct cggcgaagcg cagcatgccc
     2941 gacgcgccga tcgggttcga cgacagcacg ccgcccgaca tgttcaccgg cagctcgccg
     3001 tcgagcgcgg tggcgccctt ctcggtcatc ttccagccgt cgccctcggg cacgaagccg
     3061 aggttctcga gccacatcgg ctcgtaccag ctgaacggca cgtacatctc gatgcagtcg
     3121 atctggtcgc gcggggtggt gatgccggcc tggccgaaca cgtcggcggc gcagtccttg
     3181 cccgcctgcg ggttcacggc gtcgcgctcg gcgaacatcg tcggctcgct gcgcatcgcg
     3241 gtgccgagca cccacgccgg cggcttggc
//
LOCUS       DASPWO010000100         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_188171, whole genome shotgun sequence.
ACCESSION   DASPWO010000100 DASPWO010000000
VERSION     DASPWO010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188171"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1935
                     /locus_tag="VEO00_02255"
     CDS             <1..1935
                     /locus_tag="VEO00_02255"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HYU56859.1"
                     /translation="PEVAVAAAVATAASPFLTRRPRRLVWAAVALVSVARIYVGAHLP
                     VDTVGGFAVGWVAGALVHLAFGAPGGRPVLAAVRETLAATGLPVDDLAPAGLGRRGSA
                     VFTGHAADGTAVFCRAVGRDERDADFLDKAWRAVALRDQRDQPFFTSIQAVEHEAYLA
                     LLAERAGVRTPAVWVTAGFAGGAVLVEEQVTGTRLSAEDPTKVREAVLDDVWRAVATL
                     HAAHLTHGALRADNVYVAGARMSLVDWADGRSAATERQVAEDIAELLVSLAVRVGAKR
                     AVASAARGLGAAALVPVLPLLQPVVLSSTTRREERAHKGLLDELRAEAARVAGVDEPK
                     LEPLTRIRPETIVVVAVLALGVHLLAPQVGELGRAFEAIRESNPGWLALALVASLLTY
                     VAGGWSQTGAVRQQLALTRTTGMQLAAAFTSRLTPASLGTLAVRIRFLQRSGLSSEEA
                     VTGTALNSVAGGIARLVAIAAAIPLIGSSGFSGGVHKPADWEILAAVVVVGAIAGVVF
                     FTAIGRRLLQPIRRAFKELAVVLRDPAKALALLGGALAVIALNIICFAAALAAVHAHV
                     GASTAIVVYLGGSTVGGAAPTPGGLGAVEAALVAGLTAAGVDASLGVAGVLTFRLLTY
                     WLPIPLGYASLRVLRREGLL"
     gene            complement(1937..>2125)
                     /locus_tag="VEO00_02260"
     CDS             complement(1937..>2125)
                     /locus_tag="VEO00_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56860.1"
                     /translation="KLAARVDEITVELMSPVLGAADAGLHDLQLRIDALDQARREAHE
                     IGPDGLPRPQREQRPGTA"
BASE COUNT          218 a          800 c          803 g          304 t
ORIGIN      
        1 ccggaagtcg cggtggcggc cgcggtcgcg accgcggcgt cgccgttcct cacccgccgg
       61 ccccgccgcc tggtgtgggc ggcggtggcc ctggtctcgg tggcgcgcat ctacgtcggc
      121 gcgcacctgc ccgtggacac cgtgggcggg ttcgcggtgg gctgggtcgc aggcgcgctc
      181 gtccacctcg cgttcggtgc gcccggggga cggccggtgc tcgccgccgt gcgcgagacg
      241 ctggcggcca ccgggctccc ggtcgacgac ctcgcccccg ccgggctcgg tcgccgcggc
      301 tcggcggtgt tcaccggtca cgccgccgac gggaccgcgg tgttctgccg ggccgtcggg
      361 cgcgacgagc gcgacgccga cttcctcgac aaggcgtggc gggcggtggc gctgcgcgac
      421 cagcgcgacc agccgttctt cacctcgatc caggcggtcg agcacgaggc ctacctggcg
      481 ctgctcgcgg aacgcgccgg cgtgcgcaca ccggcggtct gggtgaccgc cgggttcgcg
      541 ggcggcgccg tcctcgtcga ggagcaggta acgggcaccc ggctgtcggc ggaggacccg
      601 accaaggtcc gagaggccgt cctcgacgat gtgtggcgcg cggttgccac tctccacgcc
      661 gcccacctca cccacggggc cctgcgggcc gacaacgtct acgtcgccgg cgcccggatg
      721 tcgctggtcg actgggccga cggacggtcg gccgcgaccg aacggcaggt ggccgaggac
      781 atcgcggagt tgctcgtgtc gctagcagtg cgggtcggcg ccaagcgcgc ggtggcgagc
      841 gcggcgcgcg ggctcggcgc ggccgcgctc gtgccggtgc tcccgctcct ccagccggtg
      901 gtgctgtcgt cgacgacccg gcgcgaggag cgagcacaca agggacttct cgacgagctc
      961 cgcgccgagg cggcgcgcgt ggccggggtc gacgagccga agctcgagcc gctgacgcgt
     1021 atccggcccg agacgatcgt ggtggtcgcg gtgctggcgc tgggcgtcca tctgctcgcg
     1081 ccgcaggtcg gggagctcgg gcgggcgttc gaagccatcc gcgagtcgaa ccccggatgg
     1141 ctcgcgctgg cgctggtggc gtcgctgctc acctacgtcg ccggcggctg gtcgcagacc
     1201 ggcgcggtgc gccagcaact cgcgctcacc cgcaccaccg gcatgcagct ggccgccgcg
     1261 ttcaccagcc ggctcacgcc cgcgtcgctc ggcaccctcg cggtccggat ccggttcctc
     1321 cagcgctccg ggctcagctc cgaggaggcc gtgaccggca ccgccctcaa ctccgtcgcc
     1381 ggcggcatcg cccggctcgt cgccatcgcc gccgccatcc ccctgatcgg ctccagcggc
     1441 ttcagcggcg gcgttcataa gcccgccgac tgggagatcc tcgccgcggt cgttgtcgtc
     1501 ggcgcgatcg ccggggtcgt gttcttcacc gcgatcggcc ggcggctgct ccagccgatc
     1561 cgccgcgcgt tcaaagagct cgcggtggtg ctccgcgacc cggccaaggc gctcgcgctc
     1621 ctcggcggcg cgctggcggt gatcgccctg aacatcatct gcttcgccgc cgcgctggcg
     1681 gcggtgcacg cccacgtggg cgcgagcacc gccatcgtcg tgtacctcgg cggcagcacc
     1741 gtcggcggcg cggctccgac gccgggcggg ctcggcgcgg tcgaggccgc gctcgtcgcg
     1801 ggactcaccg ccgccggtgt cgatgcgagc ctcggggtgg cgggcgtgct cacgttccgg
     1861 ctcctcacct actggctgcc gatcccgctc ggctatgcgt cgctgcgcgt cctgcggcgc
     1921 gaagggctgc tgtaggtcac gcggtcccgg gacgctgctc ccgctgaggc cgcggcaacc
     1981 cgtcggggcc gatctcgtgc gcctcgcggc gggcctggtc gagggcgtcg atgcgcagct
     2041 gaagatcgtg cagaccggcg tcggcggcgc cgagcaccgg gctcatcaac tcgacggtga
     2101 tctcgtcgac gcgcgccgcg agttt
//
LOCUS       DASPWO010000101         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_439545, whole genome shotgun sequence.
ACCESSION   DASPWO010000101 DASPWO010000000
VERSION     DASPWO010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439545"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VEO00_02265"
     CDS             complement(<1..420)
                     /locus_tag="VEO00_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HYU56861.1"
                     /translation="MPPRLEAVQGDITAETVDAIVNAANSSLARGGGVCGAIFRAAGP
                     ALDDACRAIGSCDTGDAVATPGFGLPTGHVIHAVGPMWNGGDAGEPELLASAYRRSLE
                     VADEIDARSVAFPAISTGIYGYPLDDATEIAVRTVKAT"
     gene            655..939
                     /locus_tag="VEO00_02270"
     CDS             655..939
                     /locus_tag="VEO00_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HYU56862.1"
                     /translation="MLAELAARAKTQGDRGWQEEANCLGVDPDLFFPERGASTREAKS
                     VCKSCEVRLECLEYALSHGEKFGIWGGLSERERRRVRRERALARRKTGVA"
     gene            complement(974..1828)
                     /locus_tag="VEO00_02275"
     CDS             complement(974..1828)
                     /locus_tag="VEO00_02275"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formiminotransferase"
                     /protein_id="HYU56863.1"
                     /translation="MLECVVNVSEGRRLDVIDAIGSACGASLLDVHCDPDHNRSVFTL
                     AGPGERDAEDAACALARAAAESLDLSRHEGVHPRLGAIDVVPFVALSGDSHLAVDAAR
                     AFAIWFADELGVPAFLYGDADPHHRALPDVRRDAFTRRPPDHGPAESHPRLGAVAVGA
                     RPPLVAVNCNLDTDDLALAREIAAQVRERDGGLPGVRALGFGLASRSRAQVSMNVTRL
                     EACGVERACTAVRRLAYEGGADVEAVELVGLVPAAELERCSPEFLTWSGLGPDQTIEA
                     RLSQDRAP"
     gene            1922..>2709
                     /locus_tag="VEO00_02280"
     CDS             1922..>2709
                     /locus_tag="VEO00_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU56864.1"
                     /translation="MQIGLALPQYDYSVPGESPLRWETLLDHAQRAEQLGFHSLWLSD
                     HLFLDIAKYGGPSDSQGAFEPLVTLAALARHVTRPRLGTLVVCEALRPAAVLAKALAT
                     LDRICGGRLDVGLGAGWYEPEYEAIGMKMPGPGERLHRLEEAVEIVKGLLGGGPLTYD
                     GRHHRAIAARNLPPALQEPRPRVFVGGKGDRLLRLVAAHADGWNTCWTWTPAAYRDRL
                     DVLERACEKEGRDPATVWRTLGLYALCGEGRADLERRFDRMREL"
BASE COUNT          382 a          980 c          999 g          348 t
ORIGIN      
        1 ggtcgccttg acggtacgca ccgcgatctc ggtggcgtcg tcgagcgggt agccgtagat
       61 gccggtcgag atggcgggga aggccacgct gcgggcgtcg atctcgtcgg cgacctcgag
      121 cgagcgccgg taggcggagg ccagcagctc gggttcgccg gcatcgcccc cgttccacat
      181 cggcccgacc gcgtggatga cgtgcccggt gggcaacccg aacccggggg tcgcaaccgc
      241 gtcgccggtg tcgcagctgc cgatggcgcg gcaggcgtcg tcgagcgcgg gcccggcggc
      301 ccggaagatg gcgccgcata cgccgccgcc gcgggcgagc gacgagttgg ccgcgttgac
      361 gatggcgtcg acggtttcgg cggtgatgtc gccctggacc gcctcgaggc gcggaggcac
      421 agccgcaggc taggcggggc cgttatccac agatgtggtc cggcgcgcaa gaaccccctt
      481 gcgctgacgg cctggaggta caagagtaac accattgtaa ttccatcggc gttattagtg
      541 agggggtcgc cgatgcgcca ggtcatcggg ccacgcgcaa agtccaacgg tgctaccaag
      601 ccgaacggaa ccaacggggc cgccggccaa gccgccgcgg ccgccactgc cgccctgctg
      661 gcggagctgg cggcccgggc caagacccag ggcgaccggg gctggcagga ggaggcgaac
      721 tgcctggggg tcgatcccga cctcttcttc cccgagcggg gagcgtcgac ccgggaggcc
      781 aagtcggtct gcaagagctg cgaggtgcgc ctcgagtgcc tcgagtacgc cctgagccac
      841 ggcgagaagt tcggcatctg gggcggcctg tcggagcggg agcggcgccg ggtgcgccgc
      901 gagcgcgccc tcgcccgccg caagaccggc gtggcctaac ccgcctcccg cggcgttgtc
      961 acacgatgcg ggactatggc gcccgatctt gtgacaaccg ggcttcgatg gtctggtcgg
     1021 ggccgagacc ggaccaggtg aggaactcgg ggctgcaccg ctcgagctcg gcggcgggga
     1081 cgagcccgac cagctcgacc gcttcgacgt cggcgccgcc ctcgtaggcc aggcgccgca
     1141 ccgcggtgca ggcccgctcg acgccgcagg cctcgagccg ggtcacgttc atcgacacct
     1201 gggcccggct gcgcgacgcg agcccgaagc ccagggcccg gactcccggg agcccaccgt
     1261 cccgctcccg cacctgcgcc gcgatttccc gggcgagggc gaggtcgtcg gtgtcgagat
     1321 tgcagttcac cgcgacgagc ggcggacggg cgccgaccgc caccgccccc agccggggat
     1381 gcgactccgc cggaccgtgg tcgggtggtc ggcgcgtgaa cgcgtcgcgc cggacgtcgg
     1441 gcagcgcgcg gtggtgcggg tcggcatcgc cgtagaggaa tgccggcacg ccgagctcat
     1501 cggcgaacca gatggcgaat gcccgggcgg cgtcgaccgc gaggtgcgag tcacccgaga
     1561 gcgcgacgaa cggaaccacg tcgatcgcgc cgagccgggg atgcacgccc tcgtgccgag
     1621 acagatcgag gctctcggcg gcggcccggg ccagcgcgca ggcagcgtcc tcggcgtcgc
     1681 gttcgcccgg cccggcgagc gtgaacaccg accggttgtg atcgggatcg cagtgcacgt
     1741 cgagcagcga cgccccgcac gcgctgccga tcgcgtcgat cacgtcgagg cggcgcccct
     1801 ccgagacgtt cacgacacac tcgagcatga cgtcaggcta cgggcgatag ccctcccgac
     1861 ctgccccacc caccgccctt cataaggaag cgcgccttcg gcgcgccgct accctcctcg
     1921 catgcagatc gggctcgcgc tgccgcagta cgactactcc gtccccggcg agtcgccgtt
     1981 gcggtgggag acgctgctcg accacgccca gcgcgccgag cagctcgggt tccattcgct
     2041 ctggctctcc gaccacctct tcctcgacat cgccaagtac ggcggcccgt ccgattcgca
     2101 gggcgcgttc gagccgctgg tgacgctggc ggcgctggcc cgccacgtca cccgtccccg
     2161 gctggggacc ctcgtcgtgt gcgaggcgct gcgcccggcg gcggtgctgg ccaaggcgct
     2221 cgccaccctc gatcgcatct gtggcggccg cctcgacgtc gggctgggcg caggctggta
     2281 cgagccggag tacgaggcga tcggcatgaa gatgccgggg ccgggcgagc ggctgcaccg
     2341 gctcgaggaa gcggtcgaga tcgtgaaggg cctgctcgga ggcggcccgc tcacctacga
     2401 cggtcggcac caccgcgcca tcgcggcccg caacctgccc ccggcgctcc aggagccgcg
     2461 cccccgcgtc ttcgtcggcg gcaagggcga ccggctcctg cgactggtgg ccgcgcacgc
     2521 cgacgggtgg aacacctgct ggacgtggac gccggccgcc taccgcgacc ggctcgacgt
     2581 cctcgagcgc gcttgcgaga aggagggacg cgacccggcc accgtctggc gcacgctcgg
     2641 cctctacgcg ctctgcggcg agggtcgcgc cgacctcgag cggcgcttcg accggatgcg
     2701 cgagctgat
//
LOCUS       DASPWO010000102         5503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_273904, whole genome shotgun sequence.
ACCESSION   DASPWO010000102 DASPWO010000000
VERSION     DASPWO010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273904"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VEO00_02285"
     CDS             <1..547
                     /locus_tag="VEO00_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYU56865.1"
                     /translation="DLGCDFYTTGVLKWLCGGPGLAVVYARRDRIPSLAPLVTGWWGQ
                     ADPFAFDTGFAYHPSARRLETGTHPAPVAFIAQGGLDLILEVGPEAIRERQGDLTESV
                     IDRADAAGIPVRTPRDRKARGGTVNLRVGPNAAEIEAALYARDVCVDARGDGIRVAPH
                     FFNDERDLDRLFEALGALLQR"
     gene            complement(567..1595)
                     /locus_tag="VEO00_02290"
     CDS             complement(567..1595)
                     /locus_tag="VEO00_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYU56866.1"
                     /translation="MRTVIRPAVVWCAPGVLHEDGRVVVEGGRVAYVGPPADAPEAEA
                     SEGGDWFLMAGGVDRHVHVGLSDPGAILLGGVVAVRDLGWPPDHIFPLADASDGPAFN
                     GPRIIAAGPMLTAISGYPTRAGWAPEGTGLEVADADGAAAAVRALADRGAGAIKVALN
                     ADAGPTLGDRELVAICQAARGRGLRVTAHVQGRGQAERALGAGVDELAHCPWEPLSDA
                     VIAGLARSVRIVSTLDMHSHGRDTPALRAAASNLRRFREAGGAVVYGTDLGNGPIPPG
                     LHAGEALHLRAAGMAPEEVLQAMTAGPLAPGAPADLVGLAGDPREDLAALGRVRFVMR
                     SGRVIRRT"
     gene            complement(1596..2498)
                     /locus_tag="VEO00_02295"
     CDS             complement(1596..2498)
                     /locus_tag="VEO00_02295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU56867.1"
                     /translation="MRVSVVIALDEDGISERHLAPIAEQRPAGVDLEVVMVDGTQPER
                     TRGEALAAIRALDGRLDARFVEHSVQGRAAAVNRGLAETTAPLVVLVGNDFVPQHGWL
                     AAHLDLHRRRPEPHVAGIGPALFPAELRTSPFLRWLEDSGRLFGVSFTRPDLPLLGLF
                     FYGANTSLKRGFLERAGAFSEAFPGEAWDDYDVGERLFAAGMEVTYLPDAVCLHEHRI
                     TVVARREQMRRAGTAAAIMERVRALPAPWNELVRVEPGRLRRSEWKRRLWAALTRSAR
                     ARAAYYRALTDRAFVDGYRRERRT"
     gene            complement(2503..4518)
                     /gene="uvrB"
                     /locus_tag="VEO00_02300"
     CDS             complement(2503..4518)
                     /gene="uvrB"
                     /locus_tag="VEO00_02300"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HYU56868.1"
                     /translation="MPPFRIVSDFKPSGHQPQAIAGLTESLRAGNRYTTLLGATGTGK
                     TATLAWTVEQVQRPTLVIAPNKSLAAQLANEFRELLPENRVEYFVSYYDYYQPEAYVP
                     QTDTFIEKDSSINDEIDRLRHSATSALLTRRDVIIVASVSCIYGLGSPEEYEGQILRV
                     HKGVPLDREWAIQRLVDIQYNRNETNFIRGKFRVRGDTIEVFPAYEETAVRIELWGDT
                     VERILRMDTVTGEVIAELPEITVYPASHYVASGDRMQRAIVSIEAELRERLAHLESEG
                     KLLEAQRLRMRTNYDLEMLREVGFCSGIENYSRHIDGRAPGEPPFTLLDYFPEDWLCV
                     LDESHVTVPQLHGMYEGDMSRKRTLVDYGFRLPSATDNRPLRFEEFEERVRQVVFMSA
                     TPGPYELRIGQATVEQVVRPTGLVDPEVRVRPTRGQVDDLIEEIRKRTEADQRVLVTT
                     LTKKMAEDLTDYLIEMGLRVRYLHSEVDTVQRIEILRDLRLGEFDVLVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSDTSLIQTIGRAARNVDGTVIMYADQVTDSMRRAIGETQ
                     RRRKIQLEHNLEHGIDPQTVRKRVSDILLSIEGERLGAPSPRRERRAARERPAMAREE
                     LERLIATLEDEMHDAARDLRFEYAARLRDEVSDLRKELRTLDRIGIA"
     gene            complement(4562..>5503)
                     /locus_tag="VEO00_02305"
     CDS             complement(4562..>5503)
                     /locus_tag="VEO00_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HYU56869.1"
                     /translation="EDGVVRAFSNVCQHRGSLLVLEPAVRGVERIVCPYHGWTYGLDG
                     SLRAAPHMAEARDFDRAACGLSAVAADTFGGWIFVNVSGDAGTLVQFLDDLPARLERF
                     RPAELRRVKRREYEVAANWKALCENYQECYHCPSIHPELVKVTPYRSGRDEPSRGPWL
                     GGPMDLADGCTTMTLTGTSARPPIPGVPEEDRRLVFYYSVLPNLWVSAHPDYVMTHTV
                     WPLAPDRTRIVCEWLFHPDQIAAPGFDPSDAVEFWDLVNRQDWAACERVQRGLASRGF
                     RGGRYSDLEGTIHRLAGLFALSYLRGRIARADELPAG"
BASE COUNT          754 a         2033 c         1953 g          763 t
ORIGIN      
        1 cgacctgggg tgcgacttct acaccaccgg cgtcctgaag tggctgtgcg gcgggccggg
       61 gctcgcggtc gtgtacgcgc gccgagaccg gatcccatcg ctcgcgccgc tcgtcaccgg
      121 ctggtggggc caggcggacc cgttcgcctt cgacaccggc ttcgcgtacc acccctccgc
      181 ccggcgcctg gaaacgggga cgcacccggc gccggtggcc ttcatcgccc agggcgggct
      241 cgacctgatc ctcgaggtcg gccccgaggc catccgcgag cgccagggcg acctgaccga
      301 gtcggtcatc gaccgcgccg acgcggcggg catcccggtg cgcacgccgc gcgaccgcaa
      361 ggcgcgcggg gggacggtca acctgcgggt cggtccgaac gccgccgaga tcgaggcggc
      421 cctgtacgcg cgcgacgtgt gcgtcgacgc tcgcggcgac gggatccgtg tggcgccgca
      481 cttcttcaac gacgagcgcg acctggaccg cctgttcgag gccctcggcg cgctgctgca
      541 acggtaaggg ggctcgcgac ccgggcctag gtacggcgga tgacccgtcc ggaccgcatc
      601 acgaaacgca cccggccgag cgcggcgagg tcctcacggg ggtcgccggc cagcccgacc
      661 aggtcggccg gcgcgccggg cgcgagcggt ccggcggtca tcgcctggag gacctcctcg
      721 ggcgccatcc cggcggcgcg gaggtgcagc gcctcgccgg cgtgcagccc cggcgggatg
      781 gggccgttcc cgaggtcggt cccgtagacg acggcgccgc cagcttcgcg gaaccggcgc
      841 aggttgctgg cggccgcgcg cagggcgggc gtgtcccgtc cgtgcgagtg catgtcgagc
      901 gtcgagacga tgcgcaccga gcgggccagc ccggcgatca ccgcatcgga caggggctcc
      961 cacgggcagt gcgccagctc gtccaccccc gccccgagcg cgcgctcggc ctgcccgcgc
     1021 ccctggacgt gcgccgtgac ccgcaggccg cgcccgcggg ccgcctggca gatcgccacc
     1081 agctcgcggt ccccgagcgt cggcccggcg tcagcgttca gcgcgacctt gatcgccccg
     1141 gcgccccggt cggccagcgc ccgcacggcg gcggccgccc catcggcgtc ggcgacctcc
     1201 agcccggtgc cctccggcgc ccacccggct cgggtcgggt agccgctgat cgcggtgagc
     1261 atcggcccgg cggcgatgat ccgcgggccg ttgaacgccg ggccgtcgct cgcgtcggcc
     1321 agcgggaaga tgtggtcggg cggccagccc aggtcgcgga ccgcgaccac cccgccgagc
     1381 aggatcgcgc cggggtccga caggccaacg tgcacgtggc ggtcgacgcc gccggccatc
     1441 aggaaccagt cgccgccctc cgacgcctct gcctcgggcg cgtcggcggg cgggcccacg
     1501 tacgcgacgc gcccgccctc caccaccact cggccgtcct cgtggaggac gcccggcgcg
     1561 caccacacga ccgccggccg gatcaccgtt cgcacctacg tcctccgctc ccgacggtat
     1621 ccgtcgacga aggcgcggtc ggtgagggcg cggtagtagg cggcccgagc ccgcgcgctg
     1681 cgcgtcaacg ccgcccacag ccggcgcttc cactcggagc ggcggagcct gcccggctcg
     1741 acccggacga gctcgttcca cggcgccggg agggctcgca cgcgctccat gatcgccgcc
     1801 gccgtccccg cccggcgcat ttgctcccgg cgggcgacca cggtgatgcg gtgctcgtgc
     1861 aggcacacgg cgtccggcag gtaggtcacc tccatgccgg cggcgaacag ccgctcgcca
     1921 acgtcgtagt cgtcccaggc ctcgcccggg aacgcctcgc tgaaggcccc cgcccgctcc
     1981 aggaatcccc gcttcagcga cgtgttcgcg ccgtagaaga agaggcccag gagcggcagg
     2041 tccgggcgag tgaacgacac cccgaagagc cgcccgctgt cctccagcca ccggaggaac
     2101 ggggaggtgc gcagctccgc cggaaacagg gccgggccga tcccggccac gtgcggctcg
     2161 ggccttcgcc ggtgcaggtc gagatgcgcg gccagccagc catgctgggg gacgaagtcg
     2221 ttccccacca ggaccacgag cggcgcggtg gtctcggcca ggccgcggtt caccgccgcg
     2281 gcgcgcccct ggaccgagtg ctcgacgaac cgcgcgtcga gccgcccatc gagcgctcgg
     2341 atggccgcga gcgcctcccc gcgggtgcgc tccggctggg tgccgtcgac catgaccacc
     2401 tcgaggtcga cgccggcggg gcgctgctcg gcgatcggcg ccaggtgccg ttcggagatg
     2461 ccgtcctcgt cgagagcgat gacgacgctg acgcgcacgc ggtcaggcga tcccgatgcg
     2521 gtcgagcgtg cgaagctcct tccgcaggtc ggacacctcg tcacgaaggc gggcggcata
     2581 ctcgaaccgg aggtcgcgcg ccgcgtcgtg catctcgtcc tcgagcgtcg cgatcagccg
     2641 ctccagctcc tcgcgagcca tcgccggccg ctcgcgcgcg gcacggcgct cgcggcgagg
     2701 ggatggtgcg ccgagccgct ctccctcgat cgagagcagg atgtcggaga cgcgcttgcg
     2761 caccgtctgc gggtcgatcc cgtgctccag gttgtgctcc agctggatct tccggcggcg
     2821 ctgcgtctca ccgatcgccc ggcgcatcga gtccgtgacc tggtctgcgt acatgatcac
     2881 ggtcccgtcc acgttccgcg ccgcccggcc gatggtctgg atcagggaag tgtcggagcg
     2941 caggaacccc tccttgtcgg cgtcgaggat cgccacgagg gacacctccg gcaggtcgag
     3001 gccctcgcgc aggaggttga tcccgaccag cacgtcgaac tcgccgaggc gcaggtcgcg
     3061 caggatttcg atgcgctgca cggtgtccac ctcggagtgc aggtagcgca cccgcaaccc
     3121 catctcgatc aggtagtcgg tcaggtcctc ggccatcttc ttggtcagcg tggtgacgag
     3181 gacccgctgg tcggcctcgg tccgcttgcg gatctcctcg atcaggtcgt ccacctgccc
     3241 ccgggtcggg cgaacccgca cctcggggtc gacgagcccg gtggggcgga cgacctgctc
     3301 gaccgtggcc tggccgatgc gcagctcgta cgggccgggc gtggccgaca tgaacaccac
     3361 ctgcctgacg cgctcctcga actcctcgaa ccgcagcggg cggttgtccg tcgccgaggg
     3421 cagccggaac ccgtagtcga ccagcgtccg cttccggctc atgtcgccct cgtacatccc
     3481 gtgcagctgc ggcacggtga cgtggctctc gtccaggacg cacagccagt cctccgggaa
     3541 gtagtcgagc agcgtaaacg gcggctcgcc cggcgcgcgg ccgtcgatgt gccgggaata
     3601 gttctcgatg ccggagcaga atcccacctc gcgcagcatc tccaagtcgt agttggtgcg
     3661 catccgcagg cgctgtgcct cgagcagctt gccctccgat tcgaggtgcg ccagtcgctc
     3721 ccgcagctcg gcttcgatcg agacgatggc gcgctgcatc cggtcgccgg acgccacgta
     3781 gtgactggcc gggtacaccg tgatctccgg cagctcggcg atgacctcgc cggtcacggt
     3841 gtccatccgc aggatgcgct cgaccgtgtc gccccacagc tcgatgcgca cggcggtctc
     3901 ctcgtaggcg gggaagacct cgatcgtgtc gccgcgcacc cggaacttgc cgcggatgaa
     3961 gttcgtctcg ttccggttgt actggatgtc caccaggcgc tggatggccc actcgcggtc
     4021 gagcggcacg cccttgtgaa cgcgcaggat ctgtccctcg tactcctccg gcgagccaag
     4081 gccgtagatg caggagacgc tggccacgat gatcacgtcc cgccgggtca ggagcgcgct
     4141 ggtggccgag tgtcgtagcc ggtcgatctc gtcgttgatc gacgagtcct tctcgatgaa
     4201 cgtgtcggtc tgcgggacgt acgcctcggg ctggtaatag tcgtagtagg agacgaagta
     4261 ctccacccgg ttctccggca agagctcgcg gaactcgttc gccagctggg cggccagcga
     4321 cttgttcggg gcgatgacga gggtgggccg ctggacctgc tcgacggtcc aggccagcgt
     4381 ggcggtcttc ccggtcccgg tggcaccgag cagcgtcgtg taccggtttc cggcccgcag
     4441 gctctcggtc agcccggcga tggcctgcgg ctggtgcccg gacggcttga agtcggacac
     4501 gatgcggaag ggaggcacgg aggcgagtgt acgacccggt accgacacgg ttccgggagc
     4561 gtcagcccgc cggcagctcg tcggcccggg cgatccgacc gcgaaggtag gacagcgcga
     4621 acaggccggc caggcggtgg atcgtgccct ccaggtcgga gtagcggccg ccacggaacc
     4681 cccggctggc cagcccgcgc tgcacccgct cgcaggccgc ccagtcctgg cggttcacca
     4741 ggtcccagaa ctccacggcg tcggacggat cgaacccggg tgcggcgatc tgatcggggt
     4801 ggaacagcca ctcgcagacg atccgagtgc ggtcgggtgc gagcggccag accgtatggg
     4861 tcatcacgta gtcggggtgg gcgctgaccc acaggttcgg cagcaccgag tagtagaaga
     4921 cgaggcggcg gtcctcctcg ggcaccccgg ggatcggcgg ccgcgcgctc gtcccggtga
     4981 gcgtcatcgt cgtgcagccg tcggcgaggt ccatcgggcc gccgagccac ggcccgcgcg
     5041 acggctcgtc ccgcccgctg cggtacgggg tcaccttgac cagctccggg tggatcgacg
     5101 ggcagtggta gcactcctgg tagttctcgc agagcgcctt ccagttcgcg gcgacctcgt
     5161 actcccgccg cttcacgcgc cgcagctcgg ccgggcggaa gcgctccagc cgggccggca
     5221 gatcgtcaag gaactgtacc aatgtgcccg cgtctcccga cacgttcacg aagatccacc
     5281 caccgaaggt gtcggccgcc accgctgaca gcccgcaggc ggcgcggtcg aagtcccgcg
     5341 cctcggccat gtgcggcgcc gcccgcagcg acccgtcgag cccgtacgtc cacccgtggt
     5401 aggggcagac gatccgctcg acgccgcgca ccgcgggttc cagcacgagc agcgacccgc
     5461 ggtgctggca gacgttcgag aacgcccgca cgaccccgtc ctc
//
LOCUS       DASPWO010000103         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_696600, whole genome shotgun sequence.
ACCESSION   DASPWO010000103 DASPWO010000000
VERSION     DASPWO010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696600"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="VEO00_02310"
     CDS             <1..1374
                     /locus_tag="VEO00_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56870.1"
                     /translation="LFSLDRAPGWDEAIYLSQVTPGAEPLLFAPSRARGITFLVAPIL
                     GLGGSVSQVRLLLVLASTAALIACFRMWVPVLGRAAPSAALVFAASWPALFYGSEVMP
                     NLWAAFPGVAACALLARRLTTGYGRYDELVAGGLVALMALVRPLDALVLTVVLVVAPV
                     LLRRGSFVWASLVSLGLLAGWAPWLVEMGGRFGGPLAALEAAARVGHTGGLSLMENVR
                     QYLALSDGPTAGPFANPHVPIVGMLWLAGLTVLLVLGILEAARRDHPATAVVPTVAGI
                     ALAAEYVVFTAVPAPRFLLPGLGLLSIPAGLGLERLLEGILHPSSTSRPRMVGAFAAV
                     IVVTAWTASQLEVATKIETGVVGHRDAAERAGAEVRRLRRNQPCQVFSSASYPMVGYA
                     SGCRASPMESAAGRWQDRAIRVEREGIRPFAVFEGEQAPAPPAGTSAVADVPAERGRT
                     WFIFVLG"
BASE COUNT          269 a          782 c          794 g          391 t
ORIGIN      
        1 ctcttctccc tcgaccgcgc gccgggctgg gacgaggcga tctacctctc gcaggtcacg
       61 cccggcgccg agccgctgct gttcgcgccg tcccgcgcgc gcgggatcac gttcctcgtc
      121 gcgccgatcc tcgggctcgg cgggtccgtg tcgcaggtgc ggctgctcct cgtcctcgcc
      181 tcgacggcgg ccctgatcgc gtgcttccgc atgtgggtcc ccgtgctcgg ccgcgcggcg
      241 ccgtcggctg cgctggtgtt cgcggcgtct tggcccgccc tcttctacgg ctccgaggtc
      301 atgcccaacc tgtgggcggc gttcccgggc gtcgcggcat gcgcgctcct cgcccgccgg
      361 ctgacgaccg ggtacgggcg gtacgacgag ctggtcgccg gcggcctcgt cgccctgatg
      421 gcgttggtcc gcccgttgga cgccctggtc cttacggtcg tcctcgtggt cgcgcctgtg
      481 ctccttcggc gaggctcgtt cgtctgggcc tcgctcgtgt ccctcgggct tcttgcgggc
      541 tgggccccgt ggctcgtcga gatgggcggc cggttcgggg gtcccctggc ggccctcgag
      601 gccgcggcgc gggtcggtca caccggtggg ctgtcgctga tggagaacgt gaggcagtac
      661 ctcgcgctga gcgacggacc gacggccggt ccgttcgcca acccgcacgt cccgatcgtc
      721 gggatgctgt ggctcgcggg cctcacggtc ctcctcgtcc tcgggatcct cgaagcggcg
      781 aggcgcgacc acccggccac cgccgtcgtc cccaccgtcg ccgggatcgc attggccgcc
      841 gagtacgtcg tcttcaccgc cgtaccggcg ccgcggttcc tcctccccgg cctggggctc
      901 ctctccatcc ccgcgggcct cggactcgag cggctcctag aagggatcct gcatccgtcg
      961 tcgacgagtc gaccgcgcat ggtgggcgcc ttcgccgcgg tcatcgtcgt cacggcgtgg
     1021 acggcgagcc agctggaggt ggcgacgaag atcgaaaccg gcgtggtcgg gcaccgggat
     1081 gcggcggagc gcgccggggc cgaggtgcgg aggctccgcc gaaaccagcc gtgccaggtg
     1141 ttcagctcgg cgagctatcc gatggtgggg tacgcgtcgg gttgccgggc gtcgcccatg
     1201 gaatcggcgg ccggcaggtg gcaggaccgc gcgatccgag tcgaacgcga aggcatccgg
     1261 ccgttcgccg tcttcgaggg cgagcaggcg cccgccccgc cggccgggac gtcggctgtc
     1321 gccgacgtgc cggccgagcg gggccgaacc tggttcattt tcgtcctcgg ctgacctgcc
     1381 gaagctcctc gcttccccgg ggatgccagc ggcccggcga cccgcccgat cagtgtggcg
     1441 gccaacggca agatccggct agacgacctt gttgccgtgg cgaatagcct gcagcctctc
     1501 taggtgacca aggggtactc tcgcgcggcg aaagggtcgc ttcccgtgga tgcgagattg
     1561 cgtttcgttc cgacgatgct gctggctctc tgcgcagcgt gcatcggcgg cgggcaaccg
     1621 cctgcatcgt cgagaagcac ccatccgcct ccctcgcctc tgcccgcgaa cgtcctctcg
     1681 cttgccccac gtaactgtcc tgtcggcctc ccgcctttgg ggcagggaga actggttcca
     1741 gtgcgcgggc tccccatgac ggtccaagaa cagcgacctc gttggatgcc tgccgggttc
     1801 ggagtcgcag gcgcgtggct cgcgggtgac ggcgatgctc agatcaagtg gtgggacggc
     1861 gcatgtcgcg aagtttcgct ctggtcgaaa gccagaatcg gcgacgtagc cggacgaccg
     1921 cgtgtgggtc tatgggtgct caggtacgac gtacaaggag gatgtggcaa tgcggtgctc
     1981 gggtcggcgc ggtgcgtcgg ctacttcgcg aacatcgacg atcgatattt cagcctccag
     2041 accatcggac tgagtcccag gtacgcagac cgagtagccc gttccgtacc cggatgatca
     2101 gggatggcac gcggagatcc tctccccctc cctgggcggg aacaggggaa ggcacggacc
     2161 agcggcgagc acgcttcagt tgggccgagt gagaccccga cgctgtagcc ggcgggcgtc
     2221 tgtcatctcc ggcccg
//
LOCUS       DASPWO010000104         3310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_439683, whole genome shotgun sequence.
ACCESSION   DASPWO010000104 DASPWO010000000
VERSION     DASPWO010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439683"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VEO00_02315"
     CDS             complement(<1..726)
                     /locus_tag="VEO00_02315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYU56871.1"
                     /translation="MVLVVKDGEAWLRPALGALARQDHRRIGVVAVDNASGDHSADIL
                     ATTLGEARVLRLPRNVGFPAAVEAALEHAGSAADADYVLLLHDDTVMAPDAISRMVEL
                     AERIDGVGVVGPKLVDWDDPTLLREVGLSADRFGYPYSPLEEEELDQGQYDRAREVLF
                     VTSAAMLVSRDAWRRAGLPDERYGSHHEDLDFCWRARLAGFRVLMSPRARARHRGATV
                     RGERKGAGRRDRRRLYAERASLAA"
     gene            complement(923..1225)
                     /locus_tag="VEO00_02320"
     CDS             complement(923..1225)
                     /locus_tag="VEO00_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56872.1"
                     /translation="MPPAKKRAAKKRTAKKSTAKRTAKRTTAKRTTKRAPAKRTAKKS
                     TAKRTTKRAPAKRTAKKRTAKKSTARKSTAKRTTAKRSARKATARKPAAKRTARTR"
     gene            complement(1266..1481)
                     /locus_tag="VEO00_02325"
                     /pseudo
     CDS             complement(1266..1481)
                     /locus_tag="VEO00_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003787027.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
     gene            complement(1719..>3310)
                     /gene="cofH"
                     /locus_tag="VEO00_02330"
     CDS             complement(1719..>3310)
                     /gene="cofH"
                     /locus_tag="VEO00_02330"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HYU56873.1"
                     /translation="SDPHYPRLLDAGIDDWGGVSPVTIDHVNPEAPWPKLDDLARRTA
                     ERGHALRERLTIYPEYAARPDPWIAGRMHAPVERLLGEDGLAADGRVPQGVPWQDPEV
                     AWKPREISLTFAKPRAAGLRDDERDVYGDWDALEVPATRDWARVRVPPDRLDADIRSA
                     LAKAADGEPDAITEPEALALYRAEGDALEALCRVADDLRRDAAGDEVTYVVNRNINFT
                     NVCYTGCRFCAFAQREVDPESYTLTLSEVGDRAEEARAYGATEVCIQGGLHPDLPGTF
                     YFDVLDELRARTPELHVHAFSPMEVLNGSVKLGISYREFLEECRMRGLGTIPGTAAEI
                     LDDEVRWVLTKGKLPADTWEEIVTTAHGLGIRSSSTIMYGHVDAPPHWVAHIRRLARI
                     QDATGGFTEFVPLPFVHQNAPIYLAGKARPGPTLAENRRMLAVARILLHGRIRNVQVS
                     WVKLGMDYCRLILQGGANDFGGTLMEETISRMAGAEHGIRKEPEEFEAAIRSIGRVPV
                     ERTTTYGRVRRHHDRRVVAAG"
BASE COUNT          428 a         1220 c         1134 g          528 t
ORIGIN      
        1 cgcggccagg ctggcccgct cggcgtacag gcgccggcga tccctccggc cggccccctt
       61 gcgttcccct cggaccgtgg ccccccggtg gcgggcccgg gcccgcggcg acatcaggac
      121 ccggaatccg gcgagccgcg cccgccagca gaagtcgagg tcctcgtggt ggctgccgta
      181 gcgctcgtcg ggcaggccgg cgcgccgcca ggcatcgcgc gagacgagca tcgccgcgga
      241 cgtgacgaac aggacctcgc gcgcccggtc gtactggccc tggtcgagct cctcctcctc
      301 gagtggcgag taggggtagc cgaaccggtc ggccgagagc ccgacctcgc gcaggagggt
      361 cgggtcgtcc cagtccacga gcttcgggcc gaccaccccg acgccgtcga tccgctcggc
      421 cagctcgacc attcgcgaga tagcgtccgg cgccatcacc gtgtcgtcgt gcaagagcag
      481 cacgtagtcg gcgtcggcgg ccgaccccgc gtgctccagg gccgcttcga cggcggccgg
      541 gaagccgacg ttgcgcggca ggcgcagtac ccgcgcctcg ccgagcgtgg tcgccaggat
      601 gtccgccgag tggtcccccg aggcgttgtc gacggccacg acgccgatcc ggcggtggtc
      661 ctgccgcgcc agcgccccga gcgcgggccg caaccaggcc tccccgtcct tgacgacgag
      721 gaccacgaga acgctcggcg ccttctgggc gctcggccgc cggggtcccg tcgtcgttgc
      781 ctgggtggac acctttgatc agatgatcgg ctgaggttcc ctggatggcg gcgcgcgggc
      841 gagcatacca gccccctgat cggcgccgga aacggcggag gggggcgtcg cccccctccg
      901 aagtgcgatg tcgccgtgcc gtctagcgcg tgcgggcggt gcgcttggcg gccggcttcc
      961 tcgcggtggc cttcctggcc gaccgcttgg cggtcgtccg cttggccgtg gacttgcgcg
     1021 cggtcgactt cttcgcggtc cgcttcttcg ccgtccgctt cgccggggcc cgcttggtcg
     1081 tgcgcttcgc ggtcgacttc ttcgccgtcc gcttcgccgg ggcccgcttg gtcgtgcgct
     1141 tcgcggtcgt cctcttggcc gtccgcttgg cggtcgactt cttggcggtc cgcttcttgg
     1201 cagcgcgctt cttcgctggg ggcatggtca cctcctctcc gctcagctcg agacgccgcg
     1261 cagggctagg acgccatgcg cttgaggcgt cgcctctccc gctcgctcat tcctccccac
     1321 acgccgtacc gctcctcgcg gcgcagcgcg tagttcaggc attcgatgcg cacgggacac
     1381 tccgcgcaga tacgcttggc ctcgcgggac gacccaccct tctccgggaa gaagatctcc
     1441 gggtcgtggt cccggcacct ggctcgctcc tgccacggga taaagtgatg caacgcggtc
     1501 cctcccccga attacattct tgtaattctg ccggaaccgc gtacgcgagc gcaagcccgc
     1561 ggggaaagaa atctcggggc cgcccagcgt tttccacacc atgcgcttca ccacacacaa
     1621 tcgtggtagg tccgcgcgtg aagtgcaaga gtttcggcac gaaaagttca atacgacatt
     1681 tggtacaggc actgtacgaa cttggtacag cgccttgact atcccgccgc caccacgcgg
     1741 cgatcatggt gacgccggac gcgcccatag gtcgtggtgc gttcgaccgg cacgcggccg
     1801 atcgagcgga tcgccgcctc gaactcctcc ggctccttgc ggatgccgtg ctcggcgccg
     1861 gccatccggc tgatcgtctc ctccatcagc gttccgccga agtcgttcgc gccgccctgc
     1921 aggatcaggc ggcagtagtc catcccgagc ttcacccacg agacctggac gttccggata
     1981 cgaccgtgga gcaggatccg ggccaccgcg agcatccggc gattctccgc gagcgtcgga
     2041 cccggacggg ccttgcccgc cagatagatc ggcgcgttct ggtggacgaa gggcagcggg
     2101 acgaactccg tgaaaccgcc cgtcgcgtcc tggatgcgcg ccaggcgccg gatgtgcgcc
     2161 acccagtgcg gcggcgcgtc gacgtgcccg tacatgatcg tcgaggagga acggatcccg
     2221 agcccgtggg cggtcgtcac gatctcctcc cacgtgtcgg ccgggagctt tcccttcgtc
     2281 agcacccagc gcacctcgtc gtcgaggatc tcggccgcgg tgcccgggat cgtccccagg
     2341 ccccgcatcc ggcactcctc gaggaactcc cggtacgaga tcccgagctt caccgacccg
     2401 ttcaacacct ccatcgggct gaaggcgtgg acgtgcagct ccggcgtgcg cgcccgcagc
     2461 tcgtcgagga cgtcgaagta gaacgtgccg ggcaggtcgg ggtgcaggcc tccctggatg
     2521 cagacctccg tggcgccgta cgcgcgggcc tcctcggccc ggtcccccac ctcggagagc
     2581 gtcagcgtgt aggactcggg gtcgacctcg cgctgggcga acgcgcagaa gcggcagccc
     2641 gtgtagcaga cgttcgtgaa gttgatgttg cggttcacga cgtaggtgac ctcgtcgccg
     2701 gccgcgtccc gccgcaggtc gtcggccacc cggcagagcg cctcgagcgc gtcgccctcg
     2761 gcgcggtaga gcgccagcgc ctcgggttcg gtgatcgcgt ccggctcgcc gtcggccgcc
     2821 ttggccagcg ccgatcggat gtccgcgtcg aggcggtcgg ggggcacgcg gacccgcgcc
     2881 cagtcccgcg tcgccggcac ctcgagcgcg tcccagtccc cgtacacgtc ccgctcgtcg
     2941 tcgcgcaggc cggcggccct cggcttggcg aacgtcaggg agatctcgcg cggcttccag
     3001 gccacctccg ggtcctgcca gggcacgccc tgtggaacgc gcccgtcggc cgccaggccg
     3061 tcctcaccga gcagccgctc gaccggggcg tgcatcctcc cggcgatcca cggatcgggc
     3121 cgggcggcgt actccgggta gatcgtcagc cgctcccgga gggcgtggcc acgctccgcg
     3181 gtccgtcgcg ccaggtcgtc gagcttcggc cagggcgcct cggggttgac gtggtcgatg
     3241 gtcaccgggg acacgccgcc ccagtcgtcg atccccgcgt cgagcagacg cggatagtgc
     3301 gggtcggaga
//
LOCUS       DASPWO010000105         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_359112, whole genome shotgun sequence.
ACCESSION   DASPWO010000105 DASPWO010000000
VERSION     DASPWO010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359112"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(395..1081)
                     /locus_tag="VEO00_02335"
     CDS             complement(395..1081)
                     /locus_tag="VEO00_02335"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HYU56874.1"
                     /translation="MRIGGISAIVGALLGMVGNLIHPVTPIDDPEGVARVIAGSDIWV
                     PVHLAIVLGIVLMLGGLVAIYHSIREGIAGALARFGLFGAVAGATIGMVLVILDGVAA
                     RQLAQEWATVPPDQKAAALSLVHANETINLALASLFNFVFAAVTFILFGLAVVFSDVY
                     PRWLGWIAFLAGLGSIAAGLIQAYVGEPTGTSRILTIIGPTVITLWLLTMGVLLARMA
                     APSRARRAPP"
     gene            complement(1167..>3077)
                     /locus_tag="VEO00_02340"
     CDS             complement(1167..>3077)
                     /locus_tag="VEO00_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HYU56875.1"
                     /translation="EFALSERLTPADEIEVEAGRWAREALQYFTRWCAEHQDDLGRIL
                     QEIDTLLAVLDRARREGPGEDFLRLARLVEPALVLGRRWGAWRHLLDQELALAATIGN
                     REAAAWAVHQLGTRALGERRVRSARRLLRRALRERKGLKDRAGARLTRHNLRLSLVPL
                     LTPFVAVTLVVLAGLGLVLVPLLGREPAPVGVTPDAVDFGDVRIGVTEQRDLTVENRG
                     DRVVRSIRVTNPAVADFAAFPRCPESLEPGRGCSVTVAFTPGTAGPIDGEMLIRFAGE
                     GGPVRVPMHGNGVPRADLPAVSLDRETVEFGDHVLNDVALEIVTVESAGQATLAIESV
                     RLEEPGEFTVSGEQTTCVTGLRLAQQETCAIAIVFVPSARGLAEGRVVIEDNATGSPH
                     GIAVNGTGVTDKPDLVVDGFSQTGPPTPGDSVAIPVEVVVLNDGDVPAGPFKVSVEFL
                     PSGSEQPFPVAFMAVPTDDVDPGGFYAFTTHDLEPDQRIVFTGVLRFQDNGSPQPGQA
                     VAIADSCVGDELIDRSVCRVTEVDEGNNRSEPLPLDVPTFVVPAPTLLTATFTNEGLL
                     LQWVPPEDVAAVAGYRIRRNGAIIGEVDPATSAFIDTSDLCEGEYLYQVVAFNGFGSE
                     SASADDTEHVVC"
BASE COUNT          523 a         1086 c         1029 g          439 t
ORIGIN      
        1 attgcccggg ttggggggac tgactagcat tggccccgtg ccgccggtct ggccactggt
       61 cgggcggcat gaggagctcg agctcatcga gcgagccatg ggccgtccac cgcccgccgg
      121 agtcgtgctg gccggggccg cgcggttctt tggaggcggc cgtccggcct cgaagtagtc
      181 ccgggccgcg aaggccatgc ctccgcacag tccgttggaa gcgtcgccga tcggcaggct
      241 caccacgccc gggatgccga tccgtcgcac cgggacgtgt gggaaggagt tggggaaccg
      301 gaagcccgag gtgctcggca ggaaccccgg aaccatgcgc ctcgccggaa tcgcctcctt
      361 tcgcaccgag ggtatcggac gactcatccc cccgttacgg aggagcccgg cgggcgcgtg
      421 acggtgccgc catgcgagcc agcaggacgc ccatcgtcaa gagccacagc gtgatgacgg
      481 tgggaccgat gatcgtcaag atccgggagg tgcccgtggg ctctccgaca tacgcctgga
      541 tcaagccggc agcgatcgag ccgagtcccg ccaggaaggc gatccatccg agccaccgcg
      601 ggtaaacgtc actgaagacc acggccagcc cgaacaggat gaaggtgacg gccgcgaaca
      661 cgaaattgaa gaggctggca agcgcgaggt taatggtctc gttcgcgtgg accaggctca
      721 gggcagcggc cttctggtcg ggtggcaccg tggcccactc ctgggccagc tgacgagcag
      781 cgactccgtc caggatgacg agcaccatgc caatggtggc gcctgccacc gccccgaaca
      841 gaccgaaccg ggccagcgca ccggcgatcc cttcacggat cgagtggtag atcgcaacga
      901 ggccgccaag catcagcacg atacccagga cgatcgcaag atggaccgga acccagatgt
      961 cgctcccggc gatgacccgc gcaaccccct cagggtcatc gatgggcgtg acgggatgga
     1021 tcagattccc gaccatgccg aggagggcgc ccacgatggc cgagattcct ccgatcctca
     1081 ttacccgtcg cacagattcg gacgtatcag ccaagcgtcc tcacctcccg tccgccttct
     1141 gccggctgag cgtggcgccg gcgaggtcag cagacgacgt gttcggtgtc gtccgcggag
     1201 gcggattcgc tcccgaaccc attgaacgcc accacctggt acagatactc gccctcgcac
     1261 aggtcggacg tgtcgatgaa ggcgctcgtt gcgggatcca cctcgccgat gatcgcgcca
     1321 tttcgccgga ttcgatagcc ggcgaccgcg gcgacgtcct ccggcggaac ccattggagc
     1381 agcagtccct cgttggtgaa cgtggccgtg agcaaagtgg gcgccgggac gacgaacgtc
     1441 gggacatcca gggggagtgg ctccgaccga ttgttgccct cgtcgacctc ggtcacccgg
     1501 cagacgctcc ggtcgatcaa ctcgtcaccc acgcagctgt ccgcgatggc caccgcctgt
     1561 cccggttgcg gactcccgtt gtcctggaac cgcagcacgc ccgtgaacac gatccgctga
     1621 tcaggctcga gatcgtgcgt cgtgaaggcg tagaagccgc ccggatcgac gtcgtcggtc
     1681 gggaccgcca tgaaggcgac cgggaaaggc tgctccgagc cggatgggag gaactcgacc
     1741 gagaccttga acgggcccgc ggggacgtcg ccgtcgttca gcaccaccac ctccacgggg
     1801 atggcgacgc tgtcgcccgg agtcggggga ccggtctggc tgaagccgtc cacgaccaga
     1861 tccggcttgt cggtgacacc ggttccgttc acggcgatcc cgtggggcga acccgtcgcg
     1921 ttgtcctcga tcaccacccg cccctcggcg agcccgcgcg ccgacggcac gaagacaatc
     1981 gcgatcgcgc acgtctcctg ctgggcaagc ctcagacccg tcacgcaggt ggtctgttcc
     2041 ccgctgaccg tgaactcccc gggctcctcg agccgcacgg actcgatcgc gagcgtcgcc
     2101 tggcccgcgc tctccaccgt gacgatctcg agcgccacat cgttgagcac gtggtccccg
     2161 aactcgacgg tctcccggtc caggctcacc gccgggaggt ccgctctcgg gacaccgttt
     2221 ccgtgcatcg gtacccgcac cgggccgcct tcgccggcaa atctgatgag catctcaccg
     2281 tcgatcgggc cggcggtgcc cggcgtgaat gccacggtga cgctgcagcc ccggcccggc
     2341 tcgagactct cggggcaacg aggaaacgcg gcgaagtccg cgaccgccgg attagtgacg
     2401 cggatcgacc tgacgacccg gtccccgcga ttctcgaccg tcaggtcacg ctgttcggtc
     2461 acgccgatgc ggacgtcccc gaagtccacg gcgtcgggcg tcacgcccac cggagcgggc
     2521 tcccggccca gaagcgggac gaggacgagg ccgaggccgg cgagcaccac cagcgtgacc
     2581 gcaacgaagg gcgtcagcag cggcacgaga ctgaggcgga ggttgtggcg tgtgagcctt
     2641 gctccggcgc ggtccttcag ccccttccgc tcccggagcg cacggcgcaa gagacgccgg
     2701 gccgagcgaa cgcggcgctc gcccagggcc cgggttccaa gctgatgaac cgcccacgcc
     2761 gccgcctccc ggttgccgat ggttgcggcc agcgccagct cctggtcgag caggtgccgc
     2821 caggctcccc aacgacgtcc caggaccagg gccggctcga ccagacgggc caggcgcagg
     2881 aaatcctcgc ccggcccctc acgacgggcc cgatccagga cggcgaggag cgtgtcgatc
     2941 tcctggagga tccggccgag gtcgtcctga tgctcggcgc accagcgggt gaaatactgg
     3001 agcgcctccc gggcccaccg gccggcctcc acctcgatct cgtccgcggg cgtcaaccgc
     3061 tcgctgaggg cgaactc
//
LOCUS       DASPWO010000106         3529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_785426, whole genome shotgun sequence.
ACCESSION   DASPWO010000106 DASPWO010000000
VERSION     DASPWO010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785426"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VEO00_02345"
     CDS             <1..456
                     /locus_tag="VEO00_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU56876.1"
                     /translation="RTVLENITMGPIEVQAVPKRRAAERARTLLQRVGIPEKADAHPA
                     DLSGGQQQRVAIARALAMEPKLMLFDEPTSALDPEMIREVLDVMRDLARDGMTMLVVT
                     HEMGFAREVCDRIVFVDAGRIVEEGPPEEFFSGAKSDRAREFVDKIIHH"
     gene            511..1410
                     /locus_tag="VEO00_02350"
     CDS             511..1410
                     /locus_tag="VEO00_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYU56877.1"
                     /translation="MRRIRVLLAVAAAFGLVAAACGSDQPSGQESPTSPVERPEFPAG
                     SLAADIVERGEIVIGVKFDVPPFGFKNPQTDEVEGFDVDLGRHVADALGVEPSFVEAI
                     SDNRIPFLKDGTVDLILSTMTITTDRDAEIDFSEPYYIAHGRVLVREDSDITGIDDLA
                     GRKVCTVTGSTYEGAVEEQAPEADTQLVDTYSECLSLIQTEAVEAVVTDDVILTGMII
                     QDPTLKLVGEEITVEPYGAGIKQGETELQDFVDEVIGAVKDDGRWADMFDTWIGQYTG
                     ESQDPPTMTLEEALELRPCEETC"
     gene            1457..2119
                     /locus_tag="VEO00_02355"
     CDS             1457..2119
                     /locus_tag="VEO00_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HYU56878.1"
                     /translation="MSELWDVLVRHFPQFRSGFLVTLRLVAISFVVAMVAGTLVGALR
                     AASSRWLRWIGAVYVEFFRNIPLLVLIFIAFLGLRGAGIRVGPWAAGTASLGLYTGAY
                     VAEAIRSGVFSVGSGQVEAGLSLGLTYRGVLRRIVLPQAVRTVIPPLGNLTIAMIKNS
                     AIVGASLLAIPDLLKEARVINNDTFQTTETFFWAAVGYLILTVSATVAVRFLERRLAV
                     RR"
     gene            2129..2932
                     /locus_tag="VEO00_02360"
     CDS             2129..2932
                     /locus_tag="VEO00_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HYU56879.1"
                     /translation="MEYLFSPDNWRWLFTGNNLRFLFEGFLVNLQIAGICMVLSLAIG
                     LLLALGRLSRSRVLRTPVGAWIDAWRNLPIIFVMLFLSLALPGSWKAAYEELAPGFLP
                     EALRTGRVLAAFLALTLYNSAVIAEIFRAGIQSLERGQGEAAAALGLTRWKAMRLVIL
                     PQGLRRMVPATVSQLITLNKDTTLVSIIAIQEVMRHGRIVTSFGFFGTTPAPVLQVFL
                     VVGVMFVVVNLTLSRLSRRLEIRQRERTGVELEAVTGVEDQAALAAEPA"
     gene            complement(3135..3272)
                     /locus_tag="VEO00_02365"
     CDS             complement(3135..3272)
                     /locus_tag="VEO00_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56880.1"
                     /translation="MGYEPTEPEPDRSSPVGYAAIKYTAIVVIVIAILLFLVWLINKI
                     F"
     gene            3391..3529
                     /locus_tag="VEO00_02370"
                     /pseudo
     CDS             3391..3529
                     /locus_tag="VEO00_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005193687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
BASE COUNT          501 a         1163 c         1304 g          561 t
ORIGIN      
        1 cgcaccgtgc tcgagaacat cacgatgggc ccgatcgagg tgcaggccgt cccgaagcgc
       61 cgcgcggccg agcgcgcccg gacgctgctg cagcgcgtcg gcatcccgga gaaggcggac
      121 gcccatcccg cggacctctc cgggggccag cagcaacgcg tggcgatcgc gcgcgccctg
      181 gcgatggagc cgaagctgat gctgttcgac gagccgacct ccgcgctcga cccggagatg
      241 atccgcgagg tcctcgacgt gatgcgggac ctggcccgcg acggcatgac gatgctcgtc
      301 gtgacgcacg agatgggctt cgcgcgcgag gtgtgcgacc ggatcgtctt cgtggacgcg
      361 ggccggatcg tcgaggaggg cccgccggag gagttcttct cgggcgcgaa gagcgaccgc
      421 gcgcgcgagt tcgtcgacaa gatcatccac cactgagacc cggcggaggg agacggagcg
      481 gccgccgcga gcggccgggg aggggggagc atgagacgga tacgggtgct gctggccgtt
      541 gcggcggcct tcgggctggt cgcggcggcg tgcgggtccg accagccgtc gggccaggag
      601 agcccgacgt cgccggtgga gcggccggag ttcccggccg ggagcctggc ggccgacatc
      661 gtcgagcgcg gcgagatcgt gatcggcgtg aagttcgacg taccgccgtt cgggttcaag
      721 aacccgcaga cggacgaggt cgaggggttc gacgtcgacc tcgggcgaca cgtcgcggac
      781 gcgctcggcg tcgagccgtc gttcgtcgag gccatctcgg acaaccggat cccgttcctg
      841 aaggacggga cggtggacct gatcctgtcg acgatgacga tcaccaccga ccgcgacgcc
      901 gagatcgact tctctgagcc gtactacatc gcgcacggcc gggtcctggt ccgcgaggat
      961 tccgacatca cggggatcga cgacctggcc ggccggaagg tctgcacggt gacgggctcg
     1021 acctacgagg gcgcggtcga ggagcaggcg cccgaggcgg acacgcaact ggtcgacacg
     1081 tactcggagt gcctcagcct gatccagacc gaggccgtcg aggcggtggt caccgacgac
     1141 gtgatcctga ccgggatgat catccaggac ccgacgctga agctggtcgg ggaggagatc
     1201 accgtcgagc cctacggcgc ggggatcaag cagggcgaga ccgagctgca ggacttcgtc
     1261 gacgaggtga tcggcgccgt gaaggacgac ggcaggtggg cggacatgtt cgacacgtgg
     1321 atcgggcagt acacgggcga gtcgcaggac ccgccgacga tgaccctcga ggaggcactg
     1381 gagctgcggc cctgcgagga gacctgctag tcgccggcgg gggcgcccgg gcgccgggcc
     1441 ccagcgagcg gtcgccgtga gcgagctgtg ggacgtgctg gtgcggcact tcccgcagtt
     1501 ccgctcgggc ttcctcgtca cgctccgcct cgtcgctatc tcgttcgtgg tggcgatggt
     1561 ggccgggacg ctcgtcgggg ccttgcgcgc ggcgtcctcg cgctggcttc ggtggatcgg
     1621 cgccgtctac gtcgagttct tccggaacat cccgctcttg gtcctgatct tcatcgcctt
     1681 cctcgggctg cgcggcgccg gtatccgcgt gggtccgtgg gccgccggga cggcgagcct
     1741 cggcctgtac acgggcgcct acgtggccga ggcgatccgg tcgggcgtgt tctccgtggg
     1801 gagcggccag gtggaagcgg ggttgtcgct cgggctgacc taccggggcg tgctgcgccg
     1861 gatcgtcctc ccccaagcgg tgcgcacggt gatcccgccg ctcggcaacc tgacgatcgc
     1921 gatgatcaag aactccgcga tcgtcggggc ctcgctgctc gcgatccccg acctgctgaa
     1981 ggaggcgcgg gtcatcaaca acgacacctt ccagaccacg gagaccttct tctgggcggc
     2041 ggtgggctac ctgatcctga cggtctccgc gaccgtcgcg gtccggttcc tggagcggcg
     2101 cctggccgtg cggcgctgag gcggaccgat ggagtacctg ttctcgcccg acaactggcg
     2161 gtggctgttc accgggaaca acctgcggtt cctgttcgag ggattcctgg tcaacctgca
     2221 gatcgcgggg atctgcatgg tcctgtcgct cgccatcgga ctgctgcttg ccctcggccg
     2281 cctgtcgcgg agccgcgtgc tgcgcacccc ggtcggggcg tggatcgacg catggcgaaa
     2341 cctgccgatc atcttcgtga tgctgttcct ctcgctggcg ctgccggggt cgtggaaagc
     2401 cgcgtacgag gaacttgcgc cgggattcct cccagaggcg ctgcgcaccg ggcgggtcct
     2461 ggcggccttc ctggcgttga cgctctacaa ctccgccgtg atcgccgaga tcttccgggc
     2521 cggcatccag tcgctcgagc gcggccaggg cgaggcggcc gcggccctcg ggctgacgcg
     2581 ctggaaggcg atgcggctgg tgatcctgcc gcagggcctg cggcggatgg tgccggctac
     2641 cgtgtcccag ctgatcacgc tgaacaagga cacgacgctc gtgtcgatca tcgccatcca
     2701 ggaggtgatg cggcacgggc ggatcgtgac gagcttcggg ttcttcggca cgacgcccgc
     2761 cccggtgctg caggtgttcc tggtcgtcgg tgtgatgttc gtcgtggtga acctgacgct
     2821 gtcccggctg tcgcggcggc tggagatccg ccagcgggag cgcaccgggg tagagctgga
     2881 ggccgtcacc ggcgtcgagg accaggccgc cctcgccgcc gaacccgcgt gaagtggtct
     2941 ctcggggcgt gaagtggcct ctcggggacc gggcgcaacc tggggccggc gcggaaggtg
     3001 ccgtacctgt tcatgaccga cgccgccggg aacctctccc tgcccgaccc gcgggcgacg
     3061 gcgggcgcgt cggcgtcacc ggcgacgtct tcatcgaacg ctacgcgatg ctggcgatcc
     3121 ggtgaccgcg gtgctcagaa gatcttgttg atgagccaga cgaggaacag caggatcgcg
     3181 atcacgatca cgacgatcgc ggtgtacttg atcgccgcgt agccgacggg gctggaccgg
     3241 tcgggctccg gctcggtcgg ttcgtatccc atctcgggtc tccttggtcg tctcgctgag
     3301 agcgagtctc cggggcgggc ggatccctgt caacgccgcc ccgcccgctc gccgttcacc
     3361 gaagcgtcat accctgagtt cgtgagcgcc gatcttcccg aggtgttgtt cgtgtgcacg
     3421 cacaacgcgg ggcggagcca gatggccgcc ggcctcctgc accagcacgc gcggggccgc
     3481 gtccacgtcc gctcggccgg gacccagccg ggcgaggtga tcaacccgg
//
LOCUS       DASPWO010000107         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_949576, whole genome shotgun sequence.
ACCESSION   DASPWO010000107 DASPWO010000000
VERSION     DASPWO010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949576"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VEO00_02375"
     CDS             complement(<1..771)
                     /locus_tag="VEO00_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56881.1"
                     /translation="MRRAAIALAVAAASVGLVIVPASDARAGGDRAGRVLIFALPAVT
                     WADVDGVELPNLERFFNHAAIADLSARASPDRRTTELGDGYVTLGAGTRAIGDGGATD
                     GEGFGVDEPFGIDTAGGVFRRRTGRSVERGIVQLGVAEIEDRNAGLLLDAEPGALGEA
                     LDDAGYSRAVIGNADGVEPAGVLPEYRRHLVSALMDERGVVPGGRVDAALLEEHARAP
                     FGLRLDHEAVGAAFEAAWTPKSVVLVEASDIVRADTYRA"
     gene            complement(768..2393)
                     /locus_tag="VEO00_02380"
     CDS             complement(768..2393)
                     /locus_tag="VEO00_02380"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HYU56882.1"
                     /translation="MTEPRSVARAAAGMGLATAVSRAFGFVRVLVIAAVLGTTYLGNT
                     FQASNSFSNVLFELLAAGALSAVLVPTFVELIDRGEEREAERLAGNVLGRGLAWLGAI
                     TVFAVVAAPWIARFLASGADNPGLRDAQVELSTFLLRFFIPQVLLYALGTVAIAALYA
                     KRRFAVTAVAPIGLTVVLVSALLVFRTLAGPGNVSLDLSFSEKLTLALGGTLGVAAFV
                     GVPAVALRLTGFRLRPRFDRGDAAIRRVLRLSGWAVFQHSMIGVLLVTAVVTGNRVEG
                     GVIAYQVAWVFFLAPYAVLAQPIHTTVLPELSIQAGRDDWAAFAGSLRWALDSMAVLV
                     IPVSVAMISLAQPGMRVVAFGEATRGGVALLAAGIASLALGLFVYGAFLLLARAYYAL
                     GDSRTPAMVALASALAGAATMAIAGRAVHGSAVVAAIGIGHSVAYGLGAAMLAIGLSR
                     RTGHAIVPTALPRALAVAAPIGLAAWWVDRALDPRGRAASLAMVAVAGIVGGLLYLVG
                     TRGFGGVAALGRGAFARVGSGGPEEADAAAEEA"
     gene            complement(2390..>2701)
                     /locus_tag="VEO00_02385"
     CDS             complement(2390..>2701)
                     /locus_tag="VEO00_02385"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HYU56883.1"
                     /translation="TDSNTELNAMVQPAASALVASEVPLVVGEMFREQEDGPDRGARV
                     AAVRGDDQLAAVVSTVDDLDLVEGQVTAVLALADLTRGVVGKYGYGAGADKSLPDPPP
                     R"
BASE COUNT          420 a         1021 c          972 g          288 t
ORIGIN      
        1 ggcgcgatag gtgtcggccc gcacgatgtc cgacgcctcc accaacacca ccgacttggg
       61 tgtccacgcg gcctcgaacg cggcgccgac cgcctcgtgg tccagccgca gaccgaacgg
      121 tgcccgcgcg tgctcttcga gcagggcggc gtcgacccgc ccgccgggca ccacaccccg
      181 ttcgtccatc aacgcgctca ccaggtgccg gcggtactcc ggcagcacgc ccgcgggctc
      241 gacgccgtcg gcgttgccga tgaccgctcg ggagtacccg gcgtcgtcga gcgcctcgcc
      301 cagcgcgccc ggttcggcgt cgagcagcag gccggcgttg cggtcttcga tctcggccac
      361 gcccagttgc acgatgccgc gctcgacgct ccgaccggtg cggcgccgga acactccgcc
      421 ggcggtgtcg atcccgaagg gctcgtcgac accgaagccc tcgccgtcgg tcgcgccgcc
      481 gtcgccgatc gcccgggtgc cggcgccgag ggtgacgtac ccgtcgccga gctcggtggt
      541 gcgccgatcg gggctggccc gcgccgagag gtcggcgatg gccgcgtggt tgaagaaccg
      601 ctcgaggttc gggagctcga cgccgtcgac gtcggcccag gtcacggcgg ggagcgcgaa
      661 gatcaggacc ctcccggcgc gatcgccgcc ggcacgggcg tcgctcgccg gcacgatgac
      721 gagccctacc gacgcggccg cgactgcgag cgcgatcgcg gcgcgcctca tgcttcctcc
      781 gcggcggcgt cggcctcctc cgggcccccc gacccgaccc gggcgaaggc gccccggccc
      841 agcgcggcga caccgccgaa gccgcgggtg ccgacgagat agaggaggcc tccgacgatc
      901 cccgcgaccg cgaccatcgc cagcgacgcc gcccgcccgc gcgggtcgag cgcccggtct
      961 acccaccagg ccgccagccc gatcggcgcc gccaccgcga gcgcgcgggg cagcgcggtg
     1021 gggacgattg cgtgcccggt gcggcgggac aaaccgatcg cgagcatcgc cgcgccgagc
     1081 ccgtaggcga cgctgtggcc gatcccgatg gcagcgacga ccgcggatcc atggaccgcc
     1141 cgaccggcga tcgccatggt cgccgccccc gcgagcgcgg atgccagcgc caccatcgcc
     1201 ggggtccggc tgtcgcccag cgcgtagtac gcgcgcgcga gaaggaggaa ggcgccgtag
     1261 acgaagagcc ccagcgccag tgacgcgatt ccggcggcga ggagggccac gccgccccgg
     1321 gtcgcttcac cgaacgcgac gacgcgcatg cccggctgcg cgagcgagat catcgccacc
     1381 gagacgggga tcacgagcac cgccatcgag tcgagcgccc atcgcagcga ccccgcgaac
     1441 gcggcccagt cgtcccggcc cgcctggatc gagagctccg ggagcaccgt ggtgtggatg
     1501 ggttgcgcga gcaccgcgta gggcgcgagg aagaacaccc acgcgacctg gtacgcgatg
     1561 acgccgcctt ccacccggtt gccggtcacg accgcggtga ccaggagaac gccgatcatc
     1621 gagtgctgga acaccgccca gcccgaaagg cgtagcaccc ggcggatcgc ggcgtcgccc
     1681 cggtcgaagc ggggacggag gcggaaaccg gtcagtcgca gcgcgaccgc gggaaccccc
     1741 acgaacgcgg cgacgcccag cgtcccgccc agggcgaggg tcagcttctc ggagaaggag
     1801 agatcgagcg agacgttgcc gggccccgcc agtgtccgga agaccagcag cgcgctcacc
     1861 agcacgacgg tcaagccgat cggtgcgacc gcggtgacgg cgaaccgtcg cttggcgtac
     1921 agcgccgcga tggcgaccgt cccgagggca tagagcagga cctgggggat gaagaagcgc
     1981 aggaggaagg tggacaactc gacctgggcg tcccgcagcc cggggttgtc ggcgccgctg
     2041 gcgaggaacc gcgcgatcca gggagcggcg acgaccgcga acacggtgat cgcgcccagc
     2101 caggccaggc cccggcccag cacgttcccg gcgagtcgct ccgcctcgcg ctcctcgccc
     2161 cggtcgatga gctcgacgaa cgtcgggacc agcaccgccg agagcgcacc ggccgccagg
     2221 agctcgaaca gcacgttgga gaacgagttc gacgcctgga acgtgttgcc gagataggtg
     2281 gtgcccagaa ccgccgcgat gacgaggacg cgcacgaacc cgaatgcccg cgagaccgcg
     2341 gtggccagtc ccatgccggc ggcggcacgg gcgaccgaac ggggctcggt caccgcggcg
     2401 gcgggtcggg caggctcttg tcggccccgg cgccgtaccc gtacttaccg acgacaccgc
     2461 gggtgaggtc ggccagcgcg aggaccgcgg tgacctgtcc ctccaccaag tcgaggtcgt
     2521 cgacggtcga gacgaccgcg gcgagctgat cgtcgccccg caccgcggcc acgcgggccc
     2581 cccggtcggg cccgtcctcc tgctcacgga acatctcccc caccaccagc ggcacctcgg
     2641 aggcgaccag ggcgctcgcc gcgggctgga ccatcgcgtt cagctccgtg ttcgagtcgg
     2701 t
//
LOCUS       DASPWO010000108         6485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_188794, whole genome shotgun sequence.
ACCESSION   DASPWO010000108 DASPWO010000000
VERSION     DASPWO010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188794"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1229
                     /locus_tag="VEO00_02390"
     CDS             183..1229
                     /locus_tag="VEO00_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019983803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYU56884.1"
                     /translation="MGLLGYFNGFGGRDMAVDLGTANTLVFHHGEGIVFNQPTVIALN
                     SRTGEVLAMGDEAWEMIGRTPAYIVAVRPLRRGAITDFDITQRMIKLILRTVGVTRFA
                     KPRVLICVPSAITEVERRAVEEAGLSAGARTVALIEEPMAAAIGAGLPTHEPIGNLIV
                     DVGGGTSEMAMVSMGGIVAMHAIRVGGFDMDAAIQQYIRREYGLAIGEKTAEQIKITT
                     GSAYPTASHRPAQVRGREVTTGMPKVIELSEDEIREALSDNVRAIVDATRLCLSGSPP
                     ELSHDVLDRGIFLTGGGGLLRGLEARLSQECEVPVHLTEQPLETVVLGAGRLLDYLPD
                     LQSAFFMNPQMGRR"
     gene            1231..2103
                     /locus_tag="VEO00_02395"
     CDS             1231..2103
                     /locus_tag="VEO00_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011900493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HYU56885.1"
                     /translation="MTFSIVAADGSGDIGVAVASKFPAVGSVVPWAVAGVGAVATQSW
                     ANTAFGPEGLRILREGASAEEAMIALLEQDRGREQRQVGVVGAAGDAASFTGLRCMEW
                     AGGRVGDGYAVQGNILTGPEVVAAMARAYEESGGELADRLLAAILAGDRAGGDRRGRQ
                     SASLCVVRPRGGYGGYTDRYVDLRVDDHSNAPTELARVFGTYDRELLIRDDPVLQATP
                     PLVADLQARLTTTGHYGGDATGRLDHPTREAISAFAGEYNLEGKLRDDDGLYQSIVRE
                     IRDVTPEIPVPDDR"
     gene            complement(2126..2467)
                     /locus_tag="VEO00_02400"
     CDS             complement(2126..2467)
                     /locus_tag="VEO00_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56886.1"
                     /translation="MRDVLVLVLAAGLSALVVWRMTIGPNPAARRRPFRRRRVRFEET
                     LEVTGASFAGVSATPAFAFVPTAREERPHRFRAVIGIATTIFLLAAGGAAALYLIGHF
                     ARLAIENLINS"
     gene            2542..3522
                     /locus_tag="VEO00_02405"
     CDS             2542..3522
                     /locus_tag="VEO00_02405"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HYU56887.1"
                     /translation="MRRSLSLIPLVVLLAVLTGVPLPLFEVGPGPAREVEPLIRVRGP
                     EVFDSAGRFLLTTVTVSRATSFEAVRGWFDRSIDIVPERDVLPPGITEQENDEIQLSE
                     MDASKLAAAYVTLQRVSGYPADHRPGALVQDVFPDFPADGRLFPGDLVLEANGEPVAG
                     RAALAAAIRRAGADGRLTLTVRAGGETRTVVVRPVRAEGVDHAVIGVSLIDNFPFSLS
                     IESGDIGGPSAGLMWALGLSDLLTPGDLAGGRVIAGTGTIDLDGAVGPIGGIEQKVVA
                     AARAGADVFLVPRRDLAGARAVVDGLELVPVDTYADALAYLFSGCRCDRF"
     gene            3577..6321
                     /locus_tag="VEO00_02410"
     CDS             3577..6321
                     /locus_tag="VEO00_02410"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU56888.1"
                     /translation="MESSTLLPAQFAVHLFALTTAVAAATVLGRRTGRPFASRAAGAL
                     GFLVLAAAQAAHGAGFAAETELWAGAARTSGYALVLLSALVAPGAGLAALVAPAAVMP
                     PAVAALAAGVAMSWRARRERGRMAMAAGLVLMGAFESVLAVPRASFSGATVTAHLLLT
                     LGWGGVAAGVVSATRRSIRFKMVAGFVAVLLVVILAVSAALTSVIAGNLRREAAERVG
                     NETGAIREEIEPLAQGQVGNVVAIAGGLDLGQPSRVLNDQLDALLTRIFRELDFIAVL
                     DRAGLVRARAGVDEVEALQLAGTDEVRSALDGTEAVSLDTLGTELLAIVGAAPVADLD
                     RPGRQVGVVVVGRRLDGDLLRLLARATPSAVVDARAGALVAETLPGFEAGDGLPRDLA
                     RRIATRVIERGEELRDTATIGSREYFLAVLPLQRQVGRVIGALLGAEPSQVLAAAERG
                     VNRVLFLVALGAVAVALMLSALFARRLSGPVRSLTAAARRVREGDLAATAPVRGEDEV
                     GDLAIAFNEMTESLGRMTHSLRDAAEEETRLREELETVLNSMGDGLIAVDPDDRVVTF
                     NPAAERITGLPVPQAMGRPVGEILVGRTKDGRALPRGGPLAAVALLRRANGETPVAIA
                     SSPIQGEGGETIGRVYVIRDRSREAEVERIKREFLATVSHELRTPLTPIVGYAELMLG
                     RDTPPERVGDFARGILESAHRLERIVGMLVDYSAIEAGRMVVEPEPTALKPIVGRAVD
                     EWRARAPGHRFVTRFEPAVPDALVDVLLFRRMIDELLDNAVKYSPQGGRVAVSLESAA
                     AGNGRPPGGRGGRPGVLVRVSDTGIGIDAKDLPGIFQDFRQLDGSDTRAYGGLGLGLA
                     FVNRIVQAHRGSIEADSRPGEGTMITIHLPAAEAGTSPVPIAQGKRDARKANRKGSRA
                     "
BASE COUNT          809 a         2284 c         2466 g          926 t
ORIGIN      
        1 ccgtggcacc gagccagcgt aagtggccgg ggtcgcccgc cagtttcaac ggcgcgcttt
       61 tggcgccctt ttgtggcttc cggcggacac gtccggggtc agacccagga cgtggccaaa
      121 acggacaccc gggccgccga agtgccggtt tttccgtttc ctggctagac tcgtgcgggc
      181 cgatgggtct ccttgggtat ttcaatggct tcggcggccg cgacatggcc gtcgacctcg
      241 gaaccgcgaa cacgctggtg ttccatcacg gcgaggggat cgtcttcaac cagccgaccg
      301 tcatcgcgct caattcacgg accggtgagg tgctggcgat gggcgacgag gcttgggaga
      361 tgatcgggcg cacgcccgcc tacatcgtcg ccgtccgccc gctgcggcgc ggcgcgatca
      421 ccgacttcga catcacgcag cggatgatca agctgatcct gcgcacggtc ggcgtgaccc
      481 gtttcgcgaa gccgcgggtg ctgatctgcg tgccgtcggc gatcaccgag gtcgagcgcc
      541 gcgccgtcga ggaggccggg ctgtcggcag gcgcgcgcac ggttgcgctg atcgaggagc
      601 cgatggccgc ggcgatcggg gccggcttgc cgacccacga accgatcggc aacctgatcg
      661 tcgacgtcgg cggcggcacg tcggagatgg ccatggtgtc gatgggcgga atcgtcgcca
      721 tgcacgccat ccgagtcggc gggttcgaca tggacgccgc gatccagcag tacatccggc
      781 gcgagtacgg tctggcgatc ggcgagaaga cggcggagca gatcaagatc accaccggtt
      841 ccgcctatcc cactgccagc catcggccgg cgcaggtgcg cggccgggag gtgaccaccg
      901 ggatgccgaa ggtgatcgaa ctgagcgagg acgagatccg cgaggccctg tccgacaacg
      961 tccgcgcgat cgtcgacgcc acgcggctgt gcctgtcggg cagcccgccc gagctgtcgc
     1021 acgacgtgct cgatcgcggg atcttcctga ccggcggagg cggcttgctg cgcggcctgg
     1081 aggcgcggct gtcgcaggag tgcgaggtgc ccgtgcacct cacggagcag ccgctcgaga
     1141 ccgtcgtgct cggcgccggc cgtctgctcg actaccttcc cgaccttcag tccgcgttct
     1201 tcatgaatcc ccagatggga cggcggtgag atgacgttct cgatcgtggc ggccgacggc
     1261 tccggcgaca tcggcgtggc cgtcgcatcg aagttcccgg cggtcggctc ggtggtgcca
     1321 tgggccgtcg ccggggtggg ggccgtcgcc acgcagtcct gggcgaacac ggcgttcggg
     1381 cccgagggct tgcggatcct gcgcgagggc gcctcggccg aggaggcaat gatcgcgctc
     1441 ctcgagcagg accggggccg cgagcagcgc caggtcgggg tcgttggcgc cgccggtgac
     1501 gccgcgtcgt tcaccggcct gcgctgcatg gagtgggcgg gcggacgcgt cggcgacggc
     1561 tacgccgtgc agggcaacat cctcaccggg ccggaggtgg tggcggcgat ggcccgcgca
     1621 tacgaggagt ccggtggcga gctggccgac cggctgctgg ccgccatcct ggccggtgac
     1681 cgggccggcg gcgaccgccg cggccgccag tcggcgtcgc tctgcgtggt ccgcccgcgc
     1741 ggcggttacg gtgggtacac cgaccggtac gtggacctgc gggtggacga tcactcgaac
     1801 gctccgactg agctcgcgcg cgtgttcggg acctacgacc gggaactgct gatccgcgac
     1861 gaccccgtcc tccaggccac cccgccgctg gtcgcggacc tgcaggcgcg cctgaccacc
     1921 accgggcact acgggggcga cgctaccggc cgcctggacc acccgacgcg cgaggccatc
     1981 tccgccttcg ccggggagta caacctggag ggcaagctgc gcgacgacga cggcctgtac
     2041 cagtcgatcg tccgcgagat ccgcgacgtc acgccggaga tccccgttcc ggacgatcgc
     2101 tagttccccg tcccgatcgg gccggctacg agttgatcag gttctcgatc gccagccggg
     2161 cgaagtgccc gatcaggtac agggccgcgg cgccgcccgc cgcgagcagg aagatcgtcg
     2221 tggcgatccc gatcacggcc cggaagcggt gcgggcgctc ctcgcgggcc gtcgggacga
     2281 aggcgaacgc cggcgtggcg gagaccccgg cgaacgacgc tccggtcacc tccagcgtct
     2341 cctcgaagcg gacccggcgc cggcggaacg ggcggcggcg cgcggcgggg ttcggcccga
     2401 tggtcatccg ccagacgacg agggcggaga gcccggccgc cagcacgaga acgagaacgt
     2461 cccgcacgtc cacaatgtac gtcgtttcgc cctcttccgg ttcgcacctc cgtctcccgg
     2521 ggcgctcgat acgatggccg cgtgcggcgg tcgctctccc tgatccccct cgtcgtcttg
     2581 ctggccgtcc tgaccggcgt tccgcttccc ctgttcgagg tcggtcccgg cccggcacgg
     2641 gaggtcgaac cgctgatccg cgtccggggc ccggaggtct tcgactcggc gggccggttc
     2701 ctgctgacca cggtgacggt ctcgcgggcc acgtcgttcg aggcggtgcg cgggtggttc
     2761 gaccgctcca tcgacatcgt gccggagcgg gacgtcctgc cgccggggat caccgagcag
     2821 gagaacgacg agatccagct ctcggaaatg gacgccagca agctcgccgc cgcgtacgtc
     2881 acactccagc gggtgtcggg ctacccggcc gaccatcgcc cgggcgccct cgtgcaggac
     2941 gtgttccccg acttcccggc ggacggacgg ctctttcccg gcgacctggt gctggaggcc
     3001 aacggggagc cggtcgccgg ccgcgcggcg ctggcggccg cgatccgccg cgccggcgcc
     3061 gacgggcggc tgaccctcac ggtgcgcgcc ggcggcgaga cacggacggt ggtcgtccgt
     3121 ccggtacgcg cggagggggt cgaccacgcg gtgatcgggg tgtcgttgat cgacaacttc
     3181 ccgttctcgc tctccatcga gagcggcgac atcggcgggc cctcggccgg gctgatgtgg
     3241 gccctcggcc tgagcgacct gttgacgccc ggcgacctgg ccgggggccg ggtgatcgcc
     3301 gggaccggga cgatcgacct cgacggcgcg gtcgggccga tcggggggat cgagcagaag
     3361 gtggtggcgg cggcgcgggc cggcgcggac gtgttcctgg tcccgcgacg ggacctcgcc
     3421 ggtgcccggg cggtcgtcga cgggctggag ctcgttccgg tcgacaccta tgccgacgcg
     3481 ctggcgtacc tgttctccgg ctgccgctgc gatcgcttct gacctgtatc gccgcaggta
     3541 gggcggcgta tactcggtca cccgcaacgg gcaacgatgg aatcgagcac cctcctgccg
     3601 gcgcagttcg ccgttcacct gttcgccctc accacggccg tcgcggcggc aaccgtgctc
     3661 ggacggcgga cgggtcgccc gttcgcgtcg cgcgcggccg gcgcgctcgg cttcctggtc
     3721 ctcgccgcgg cccaggccgc ccacggggcc ggcttcgccg ccgagacgga gctctgggcc
     3781 ggcgcggcgc ggaccagcgg gtacgccctc gtgctcctct cggccctcgt cgcccccggg
     3841 gccggtctcg cggcgctggt ggcgcccgcg gcggtgatgc cgccggcggt cgccgcgctg
     3901 gcggccggcg tcgcgatgag ctggcgggcc cgccgcgagc gcgggcggat ggcgatggcc
     3961 gccgggctgg tcctgatggg agcgttcgag tccgtgctgg cggtcccgcg cgcctcgttc
     4021 tccggggcca ctgtcaccgc ccacctcctg ctgacgctcg ggtggggagg ggtcgccgcc
     4081 ggggtcgtct ccgcgacccg ccgcagcatc cgcttcaaga tggtcgccgg gttcgtcgcg
     4141 gtgctgctcg tggtgatcct ggccgtctcg gctgccctga cgagcgtcat cgccgggaac
     4201 ctgcgccgcg aggcggccga gcgcgtgggc aacgagaccg gcgcgatccg ggaggagatc
     4261 gagccactgg cgcaggggca ggtcgggaac gtggtggcca tcgccggggg actggacctc
     4321 gggcagccct cgagggtcct gaacgaccag ctcgacgcgc tcctgacccg gatcttccgc
     4381 gagctggact tcatcgccgt gctggatcgg gcggggctcg tgcgggcccg ggccggcgtg
     4441 gacgaggtcg aggcgctcca gctcgccggg accgacgagg tccggtcggc gctcgacggg
     4501 accgaggccg tctcgctcga cacgctcggg accgagctgc tggcgatcgt cggggccgcg
     4561 ccggtggccg acctcgaccg gccgggccgc caggtagggg tggtggtcgt cggccgccgg
     4621 ctcgacggcg acctgctccg gttgctggcg cgggccacgc cgtcggccgt ggtcgacgcc
     4681 cgggccgggg cgctcgtggc cgagaccctc ccggggttcg aggccggcga cggcctgccg
     4741 cgggacctgg cccgccggat cgcgacccgg gtgatcgagc ggggcgagga gctccgggac
     4801 accgcgacga tcgggtcgcg ggagtacttc ctggccgtgc tcccgctgca gcgccaggtc
     4861 ggccgggtga tcggagcgct cctcggcgcc gagccatcgc aggtgctggc cgcggccgag
     4921 cggggcgtga accgcgtgct gttcctggtg gccctcggtg ccgtcgccgt ggcgctgatg
     4981 ctctccgccc tgttcgcccg ccggctgtcc ggtccggtcc ggtcgctgac cgccgccgcg
     5041 cgacgggtgc gggagggcga cctggcggcc accgctccgg tgcgtggtga ggacgaggtg
     5101 ggcgacctgg cgatcgcgtt caacgagatg accgagtcgc tcgggcggat gacccattcg
     5161 ctccgggacg ccgcggagga ggagacccgg ctgcgcgaag agctcgagac cgtcctgaac
     5221 tcgatgggcg atggactgat cgccgtggac ccggacgatc gcgtcgtcac cttcaacccg
     5281 gccgcggagc ggatcaccgg gctcccggtg ccccaggcga tgggccggcc ggtcggcgag
     5341 atcctcgtcg ggcgaaccaa ggacggccgc gcgctgcccc gcggcggccc gctggcggcc
     5401 gtcgccctcc ttcgccgggc aaacggtgag acgccggtgg cgatcgcctc gagcccgatc
     5461 cagggggagg gcggcgagac gatcggccgg gtctacgtca tccgcgaccg cagccgcgag
     5521 gccgaggtgg agcggatcaa gcgggagttc ctggcgacgg tgtcccacga gctccgcacg
     5581 cccctcaccc cgatcgtcgg gtacgccgag ctgatgctcg gacgcgacac cccgcccgag
     5641 cgggtcggcg acttcgcccg cgggatcctg gagtcggcgc accgcctgga acggatcgtc
     5701 gggatgctcg tcgattactc ggcgatcgag gcggggcgca tggtggtcga gccggagccg
     5761 accgccctaa agccgatcgt cggccgcgcg gtggatgagt ggcgagcccg cgcccccggc
     5821 caccggttcg tgacgcgctt cgagcccgcc gtccccgacg cactggtcga cgtccttctc
     5881 ttccggcgga tgatcgacga gctgctcgac aacgccgtga agtactcgcc gcagggcggg
     5941 cgggtcgccg tgtcgctcga gtccgccgca gccgggaacg gccggccgcc tggcgggcgg
     6001 ggggggcggc ccggggtgct ggtgcgggtc agcgacacgg ggatcgggat cgacgcgaag
     6061 gacctgccgg gcatcttcca ggacttccgc cagctggacg gttcggacac ccgcgcctac
     6121 ggcgggctgg ggctgggcct cgccttcgtc aaccggatcg tccaggcgca ccgcggctcg
     6181 atcgaggccg actcgcgacc gggcgagggg acgatgatta caattcacct gcccgccgcc
     6241 gaggcaggaa cgtccccggt gcccatcgcc cagggcaagc gcgacgctcg gaaggcgaac
     6301 aggaagggaa gccgcgcgtg agacgcatgg cgctcgcggt ggtgggcgcg atcctcttcg
     6361 gggcgtgctc cgccggtccc tccggcggcg ccgggtcggc gcgcgccgtt ccgctgggcg
     6421 gcatcgtcga ggtgggccac gacggggcct ggcgtgccct ggagagcccc tccgacctgg
     6481 agccc
//
LOCUS       DASPWO010000109         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_274584, whole genome shotgun sequence.
ACCESSION   DASPWO010000109 DASPWO010000000
VERSION     DASPWO010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274584"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VEO00_02415"
     CDS             <1..268
                     /locus_tag="VEO00_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYU56889.1"
                     /translation="VSERMTPDLITVDPNTSLRDAARLMADRWIRHLPVLEQGKVVGV
                     VSQRDLAGLVAGALNEPETLEQLIESSELVRERRLRRIEHGTWD"
     gene            321..>2363
                     /locus_tag="VEO00_02420"
     CDS             321..>2363
                     /locus_tag="VEO00_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56890.1"
                     /translation="MDRSSGPAVRLTLVPHTHWDREWYLPFEGFRERLVRMMDGLLAL
                     ADRGFPHFHLDGQVAMIEDYLAVRPERADDLARHVREGRLSAGPWFTQMDEFLVSGES
                     HVRNLERGLARARELGRALEVGYLPDQFGHIGQMPQLLRAAGIDRAVVWRGVPASIDR
                     TAFRWESPDGSRVLAEYLAFGYGLGGGLRDAETPQELAEGVRGAVDRLRPLSPRDGLL
                     VMAGADHHGPDPTLPERLAATASLLPAVEARVGSLEDHLARPMVRDLPVWRGELRSSA
                     RAHLLPGVVSARIHQKRERARVEALVERYAEPLAALVPGGEWPHRELERAWTLLLWNG
                     AHDSVCGCSVDEVAAAVEARLAEARAIAEDVLRSSLERLAASVAPSGTLRFNPSPFER
                     KGVPGLGWDVAPAPIPPPGEPAELDIRGDHLVVDGVPIRLFDEPDVGDLYNFCPAEGG
                     APRPPDRMTAGTGLVWAGWESLAVGVSAIRRDGIVVLDLTIRNARPDHRLRLSAGLPR
                     RADRAVALSPFEVVERPLVSEGSSLEAPSPTWPARGAVLAGGLAVLAEGVFEYEVVDG
                     KELAVTLLRCVGTISRPSLATRPWPAGPDVPTPRAQMLGETNLALALLPNAGPRDLVP
                     AWERLALPLATATAPGGGALAGTGSLLEVIGAALSSVRHRGDALEARLWNPGHAPAR"
BASE COUNT          264 a          892 c          892 g          315 t
ORIGIN      
        1 ggtctcggag cggatgacac cagacctgat cacggtcgat ccgaacacgt cgctgcgcga
       61 cgccgcccgc ctgatggccg atcgctggat ccggcacctc ccggtgctcg agcaggggaa
      121 ggtcgtcggc gtcgtctcgc agcgcgacct ggccgggctg gtggccggcg ccctgaacga
      181 gccggagacg ctggagcagc tgatcgagtc cagcgagctg gtccgcgagc ggcgcctccg
      241 ccggatcgag cacgggacct gggactagcc ccaccacggg gggggctccg ccccccgtgt
      301 acccccggaa ggcaggagac atggaccgtt ccagcggacc cgcggtccgc ctgacgctcg
      361 tcccgcacac gcactgggac cgcgagtggt acctcccgtt cgagggcttc cgcgagcggc
      421 tggtgcggat gatggacggc ctgctggcgc tggccgaccg cgggttcccg cacttccacc
      481 tcgacggcca ggtggccatg atcgaggact acctcgcggt ccgtcccgag cgcgccgacg
      541 acctggcccg ccacgttcgc gagggccgcc tctccgccgg gccgtggttc acgcagatgg
      601 acgagttcct ggtgtccggc gagtcgcacg ttcgcaacct ggagcgcggg ctggcgcgcg
      661 cccgagagct cgggcgggcg ctggaggtcg gctacctccc ggaccagttc ggccacatcg
      721 ggcagatgcc gcagctgctg cgcgccgccg gcatcgaccg cgcggtcgtc tggcgcgggg
      781 tcccggcctc gatcgaccgc accgccttcc ggtgggaatc gcccgacggg agccgcgtgc
      841 tggccgagta cctggcgttc ggttacgggc tcggcggcgg gctccgcgac gccgagacac
      901 cgcaggagct ggccgagggc gtgcgcggcg ccgtcgaccg gctgcgaccg ctgtcgccgc
      961 gcgacggcct gctcgtgatg gccggggccg atcaccacgg gcccgacccg acgctccccg
     1021 agcgcctggc ggccaccgcc agcctccttc ccgccgtcga ggcgcgtgtg gggtcgttgg
     1081 aggaccacct ggcccgcccc atggtgcgcg atctccccgt ctggcgcggc gagctccgct
     1141 cgtcggcgcg tgcccacctg ctacccgggg tcgtctcggc gcggatccac cagaagcggg
     1201 agcgggcgcg ggtcgaggcc ctggtcgagc gctacgccga gcccctggcg gcgctggtcc
     1261 ccggcggcga gtggccgcac cgggagctgg agcgtgcctg gacgctcctg ctctggaacg
     1321 gggcgcacga ctcggtgtgc ggctgctcgg tggacgaggt cgcggcggcc gtcgaggcgc
     1381 gcctggccga agcgcgcgcg atcgccgagg acgtcctccg ctcctccctc gagcgcctgg
     1441 ccgcgtcggt ggcaccgtcc gggacgctgc gattcaaccc gtcgcccttc gagcgcaagg
     1501 gcgtccccgg cctgggatgg gacgtggctc ccgcgcccat cccgccgccc ggcgagccgg
     1561 cggagctcga catccggggc gaccacctcg tggtggacgg ggtcccgatc cggctgttcg
     1621 acgagcccga cgtgggcgac ctctacaact tctgcccggc ggagggcggc gcgccgcggc
     1681 cgcccgaccg catgaccgcc ggcaccggcc tcgtctgggc cggatgggag agcctcgcgg
     1741 tcggcgtctc ggcgatacgg cgggacggca tcgttgtgct cgacctaacg atacgcaacg
     1801 cgcgaccgga ccaccgcctg cgcctctccg ccggcctgcc gcgtcgcgcg gaccgcgcgg
     1861 tcgccctctc gccgttcgag gtcgtggagc ggccgctcgt ctccgagggc agctcgctgg
     1921 aggccccgtc gccgacctgg ccggcgcggg gagcggtgct ggccggcggc ctggccgtcc
     1981 tggccgaggg cgtgttcgag tacgaggtcg ttgacgggaa ggagctggcg gtgacgctgc
     2041 ttcgctgcgt cgggaccatc tcccggccat cgctcgccac acggccgtgg ccggccggtc
     2101 cggacgtgcc gaccccacgg gcgcagatgc tcggcgagac gaacctggcg ctcgcgttgc
     2161 tgccgaacgc cggcccacgc gacctggtgc cggcctggga gcggctggcg ctccctctgg
     2221 ccaccgcgac ggcgccgggc ggcggcgccc tggccgggac cggctccctg ctggaggtca
     2281 tcggggccgc cctctcgtcc gtgcggcacc gcggggacgc gctcgaggcg cgcctctgga
     2341 accccggcca cgctcccgcg cgg
//
LOCUS       DASPWO010000110         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1120166, whole genome shotgun sequence.
ACCESSION   DASPWO010000110 DASPWO010000000
VERSION     DASPWO010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120166"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VEO00_02425"
     CDS             <1..793
                     /locus_tag="VEO00_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HYU56891.1"
                     /translation="LAASVVGLTAPAIGTLLVQRRLSLIGDGMGHLAFAGVALGVLAN
                     VTPLWGAFVAAALGALGLERLRMRGRLSGDLSMALVFYFGIALGAVVLSALGRLDAFA
                     IGILFGSVYTVSTADLIVMVVLCVVEVLAIVVLYKELLAVALDEESARAAGLPVDGLN
                     MLTVGMTALLVVAGMRAVGLLLISALLVVPVAASSRIAHSFRGAMLIAMGLGLASALV
                     GLVVALAQGDVAPSGTIVLAALVFFGLSMLLGRRAFRRHPRRVPG"
     gene            790..1221
                     /locus_tag="VEO00_02430"
     CDS             790..1221
                     /locus_tag="VEO00_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HYU56892.1"
                     /translation="MNREGALLGDAGLRPTRQRLAILRSVAAERRPVTAQDLYARLRG
                     SGPSPGLATVYRTLRSLADAGVLRTFPAGEGEVAYRLCEPGHHHHLICEGCGQVVEIP
                     SCEVEEWASGVAERRGFTAAHHQADIFGLCPKCRAAASIRG"
     gene            complement(1230..1691)
                     /locus_tag="VEO00_02435"
     CDS             complement(1230..1691)
                     /locus_tag="VEO00_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYU56893.1"
                     /translation="MSEPAGREAWRAMLALFFSGQAHERMQEASAAVGLAPGVMKTLL
                     QLEPGDGVPLRDLADYWRCDASYVTSVADALEERGLAVRRPHATDRRIKMLVLTADGI
                     AARERASRVLHEPPPSFGALTVAEQRQLRGLLRKVADADAPLHRERPAAVR"
     gene            1788..>2349
                     /locus_tag="VEO00_02440"
     CDS             1788..>2349
                     /locus_tag="VEO00_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006142595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU56894.1"
                     /translation="MPFQTDQARGNGHAPGAARRQVHPVVALMVIAGAQLMVVLDVTI
                     VNVALPHIRSGLGFSRTGLAWVIDAYTLAFGGLLLLGGRAGDILGRRRMFMIGVALFA
                     GASLAGGFATSSAWLIAARAAQGVGAAIASPTALSLVTTAFPEGPERNRAFGIYAAVA
                     GAGGGIGLILGGVLTDLLSWRWVLFVN"
BASE COUNT          263 a          836 c          867 g          383 t
ORIGIN      
        1 cctggcggcg tccgtggtcg ggctcacggc gcccgccatc gggacgttgc tcgtgcagcg
       61 aaggctgtcg ctgatcggcg acggcatggg ccacctggcg ttcgccggcg tggcgctcgg
      121 ggtcctggcg aacgtgacgc cgctgtgggg cgcattcgtc gcggcggcgc tcggggcgct
      181 ggggctcgag cggctccgga tgcgggggcg cctgtccggc gacctgtcga tggccctggt
      241 gttctacttc gggatcgccc tgggggccgt ggtcctgtcc gcgctcggcc gcctcgacgc
      301 cttcgccatc ggcatcctgt tcggaagcgt gtacacggtc tccacggcgg acctgatcgt
      361 catggtcgtc ctctgcgtcg tcgaggtgct ggcgatcgtc gtcctgtaca aggagctgct
      421 ggcggtggcc ctggacgagg agagcgctcg cgccgccggc ctccccgtgg acggcctgaa
      481 catgctcacc gtggggatga ccgcgctgct ggtggtggcg gggatgcggg cggtggggct
      541 gctgctgatc tcggctcttc tggtggtgcc cgtcgccgcc agctcgcgca tcgcgcactc
      601 gttccgggga gccatgctca tcgccatggg cctgggcctc gcttcggcgc tggtcggcct
      661 ggtggtggcg ctcgcgcagg gggacgtggc cccgagtgga acgatcgtcc tggccgcgct
      721 ggtgttcttc gggctctcga tgctgctggg acggcgggcc tttcggcggc atccgcggag
      781 ggttcccgga tgaaccgcga gggagcgctc ctgggcgacg ccgggctccg gcccacgcgg
      841 cagcgcctgg ccatcctgcg ctcggtggcg gccgaacggc ggcccgtcac cgcccaggac
      901 ctctacgccc gccttcgcgg ctccgggccg agcccggggc tggctaccgt gtaccggacg
      961 ctccggtcgc tggccgacgc gggagtcctg cggacgttcc cggccggcga gggagaggtc
     1021 gcgtaccggc tgtgcgaacc cggccaccac caccacctga tctgcgaggg atgcgggcag
     1081 gtcgtggaga tcccgtcctg cgaggtcgag gagtgggcgt ccggcgtggc cgaacggcgc
     1141 ggcttcaccg cggcccacca ccaggcggac atcttcggcc tgtgcccgaa atgccgcgcc
     1201 gccgcgtcga tccgcgggta gacgaggcgt cagcgaacgg cggcggggcg ctcgcggtgc
     1261 aggggcgcgt ccgcgtcggc gacctttcgc agcaggccgc ggagctggcg ctgctcggcc
     1321 acggtcaggg cgccgaacga gggcggcggc tcgtgcagca cgcgggaggc ccgttcccgc
     1381 gccgcgatcc cgtcagccgt gaggaccagc atcttgatcc gccggtccgt ggcatggggc
     1441 cggcgcaccg cgaggccccg ttcctcgagc gcgtcggcca cgctggtgac gtacgacgcg
     1501 tcgcaccgcc agtagtcggc gaggtcgcgc agggggacgc cgtcacccgg ctcgagctgg
     1561 aggagcgtct tcatcacgcc gggggcaagc ccgaccgcgg cgctcgcctc ctgcatccgc
     1621 tcatgggcct gcccgctgaa gaacagggcc agcatggccc gccacgcctc ccgtcctgcc
     1681 ggctcactca tgttccgagc gtagctcatt cgtccaccac cgtccatcat ttacttctct
     1741 gaagtaagcg tttgacactt atcaaacgtt gagtataccc tacggccatg ccttttcaaa
     1801 ccgaccaagc acgcgggaac ggccacgctc ccggcgcggc acgacggcag gtccatccgg
     1861 tggtggccct catggtcatc gccggcgcgc agctcatggt ggtcctcgac gtgacgatcg
     1921 tgaacgtggc cctcccccac atccggtccg ggctcgggtt ctcgcggacc ggcctggcct
     1981 gggtcatcga cgcctacacg ctcgccttcg gcggcctgct gctgctgggc ggcagggccg
     2041 gcgacatcct gggccggcgc cggatgttca tgatcggcgt ggccctgttc gcgggggcca
     2101 gcctcgccgg cggcttcgcc accagcagcg cctggctcat cgcggcccgc gcggcgcagg
     2161 gggtcggcgc ggccatcgcg tcgccgaccg cgctctcgct cgtgaccacg gccttcccgg
     2221 aaggtcccga gcgcaaccgc gccttcggca tctacgcggc cgtggccggc gccggcggcg
     2281 ggatcggcct gatcctgggc ggggtgctca ccgacctgct ctcctggcgg tgggtgctgt
     2341 tcgtcaacg
//
LOCUS       DASPWO010000111         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_786286, whole genome shotgun sequence.
ACCESSION   DASPWO010000111 DASPWO010000000
VERSION     DASPWO010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786286"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VEO00_02445"
     CDS             complement(<1..799)
                     /locus_tag="VEO00_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYU56895.1"
                     /translation="MVRPEDFRRAIERGYTAAPEESVPFYDAIVGADRCRKDPAHCDL
                     SEGIQTSESTVTFRLTKPDPDFLYKLALPFASAVPSATGDAEARAPLPATGPYMFESY
                     DPKEGLVLVRNPHFREWSPVAQPDGYPDRIEWTFGVTVREARDRVLEGEADWADFGFT
                     GSVPGLDEVMRKAPELAHPYLTTAAFYMSLNTTKPPFDDVRVRRALNLAIDRNKIVEL
                     SGGPDRARPTCQAFPPSFPGYEPYCPYTVDPDGDWTAPDFARAEELIR"
     gene            complement(1277..2035)
                     /locus_tag="VEO00_02450"
     CDS             complement(1277..2035)
                     /locus_tag="VEO00_02450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYU56896.1"
                     /translation="MSFDVAANAYDRFMGRYSSKLAPAFVDFAGVNAGQTVLDVGCGP
                     GAATAELVRRLGQGAVAAVDPSERFVAAARVRNPRVDVRLASADDLPYPDGTFDATIA
                     QLVVHFMTDPVAGLAEMARVTGAGGVVAACVWDLAGGRAPVSTFWRAAQELHDHVQDE
                     SGLPGARAGHLSELFEAAGLRRVEEAALSASADHPSFDDWWEPFTFGVGPAGEYARTL
                     GETELSALRERCRHLLPAGPFTLTAVAWAARGLA"
     gene            2252..2605
                     /locus_tag="VEO00_02455"
     CDS             2252..2605
                     /locus_tag="VEO00_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYU56897.1"
                     /translation="MTRYLLSIYQPDGAPPPSVDLEKVMRDVADLVQEVKAGGAWVFN
                     GGLHPASTATVVRLKDGDLLMTDGPFTEGKEHVGGLMIVRAPDLDAALEWAGKAARAT
                     TLPIEVRPFQEEVED"
BASE COUNT          420 a          929 c          928 g          380 t
ORIGIN      
        1 ggcggatgag ctcctcggcc cgggcgaagt ccggcgccgt ccagtcccca tccggatcca
       61 ccgtgtacgg gcagtacggc tcgtagccgg ggaagctcgg ggggaaggcc tggcacgtcg
      121 ggcgtgcgcg gtccggcccg ccggagagct ccacgatctt gttccggtcg atcgcgaggt
      181 tcaacgcccg gcggacgcgc acgtcgtcga agggcggctt cgtcgtgttc agggacatgt
      241 agaacgcggc ggtcgtcaga tagggatggg ccagctccgg agccttgcgc atcacctcgt
      301 cgagtcccgg cacgctcccc gtgaatccga agtccgccca gtccgcctca ccttcgagga
      361 cccggtccct ggcctcccgc acggtgaccc cgaaggtcca ctcgatccgg tcgggatacc
      421 cgtcgggctg cgccacgggg gaccactcgc ggaaatgagg gttgcggacg aggacgagcc
      481 cttccttcgg gtcataagac tcgaacatgt aggggcccgt cgcggggagt ggcgcgcgcg
      541 cctcggcgtc gccggtcgcg gacggcacgg ccgacgcgaa ggggagggcc agcttgtaga
      601 ggaagtcggg atcaggcttc gtcagccgga acgtgaccgt ggactccgat gtctggatcc
      661 cctcggagag gtcgcagtgg gccggatctt tccggcaccg gtcggcgccg acgatggcgt
      721 cgtagaaggg gacgctctcc tcgggcgccg ccgtatagcc gcgttcgatg gcccggcgga
      781 agtcctcggg cctgaccacc tcgccggtcg agtatcggat gccgggccgc gatgcggtac
      841 agggcgtagc gcacggtgat cggcccgagc cgcttcgtga tggcaccggg ggaatcgaaa
      901 cggcgatcga ggtccgcctc cgtcagggtc tcgaggtacc ggagcgcggc cccgcgaatc
      961 tccttcgtgg cggcgacgat cgggtcccag tcgtcggcca cacccgcgct ccccatgcgg
     1021 aaccggtcgg caccgaggaa cgcatgacgg tccctccccc gcaggccgtg attgaccatc
     1081 cgatcgacat gctcggtgac gtgcccgagc gtccacgcga tcgagctctg tccctccgga
     1141 ttgcgaacgg cttcctcggc gctgaccccg tccaccacgc gatcgaagtc gctccaggcc
     1201 tcgatcacga gtccggccgc ggggatcacg ttcgaacgat acgtcgtcgt gcgaaccgtg
     1261 ggacgaggaa cggagctcat gcgaggcccc gtgcggccca ggcaacggct gtcagcgtga
     1321 aggggcctgc tggcagcagg tgccggcagc gctcgcgaag tgcggacagt tccgtttcgc
     1381 cgagcgttcg tgcgtattcg cctgcgggtc ccacgccgaa tgtgaacggc tcccaccagt
     1441 cgtcgaagct cgggtgatcg gcggacgcgg agagcgcggc ttcctcgacg cggcgcaacc
     1501 ccgccgcctc gaacagctcc gagaggtggc cggctcgcgc acccgggaga cccgattcgt
     1561 cctggacgtg atcgtggagc tcctgagccg cccgccagaa cgtggagacc ggggctcgcc
     1621 cacccgccag atcccagaca caggccgcga ccacgccgcc cgccccggtc acgcgggcca
     1681 tctcggcgag cccggccacc ggatccgtca tgaagtgcac cacgagctgc gcgatcgtcg
     1741 cgtcgaacgt gccgtccgga tagggcaggt cgtcagcgga tgccagccgc acgtcgacac
     1801 ggggattccg gacccgcgcc gcggcgacga accgctccga cgggtcgacg gccgccaccg
     1861 caccctgccc gagccgccgg acgagctcgg ccgtcgcagc ccccggcccg catcccacgt
     1921 ccagcaccgt ctgtccggcg ttcacgcccg cgaagtcgac gaacgccggc gcgagcttcg
     1981 acgagtaccg gcccatgaac cggtcgtagg cgttcgccgc gacatcgaag ctcatgcgtc
     2041 gatctcgaag taggcgtgct gccctgccag ccgaaccccc gggcaccgcc tgctccgcca
     2101 tcaccgcgaa gcgtacgggg cagcggatat gcgctgcagc acccgccatg cgatcgtcga
     2161 attgtcgatc cgggcggccg ccgtttgtct acatggtgag ggtccccgcc gattcccgag
     2221 caatgacggc gcccgtgaag gaggaaccga gatgacgcgc tacctgctca gcatctatca
     2281 gccggacgga gctccgccac cgtccgtgga ccttgagaag gtgatgcgcg acgtcgccga
     2341 cctggtccag gaggtcaagg ccgggggcgc gtgggtcttc aacggcggcc tccacccggc
     2401 gagcacggcc accgtcgtgc ggctgaagga cggcgacctg ctgatgaccg acggaccgtt
     2461 caccgagggg aaggagcacg tcggtggcct catgatcgtg cgggcgccgg atctcgacgc
     2521 cgcgctcgag tgggccggca aggccgcccg ggcgaccaca ctcccgatcg aggtgcggcc
     2581 gttccaggag gaggtcgagg actgacaccc gaggcaccgg tcgccatctc ggagatcgag
     2641 cgtgtgttcc gcgagga
//
LOCUS       DASPWO010000112        14748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_950367, whole genome shotgun sequence.
ACCESSION   DASPWO010000112 DASPWO010000000
VERSION     DASPWO010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14748
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950367"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1984)
                     /locus_tag="VEO00_02460"
     CDS             complement(176..1984)
                     /locus_tag="VEO00_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HYU56898.1"
                     /translation="MRARILAAASLVAALAVAVPSPAGAASSWTRWSPRPATYGVART
                     ADVPIRMSDGVVLYADVLRPARADGTPAPGRFPVVLTQTPYNKNGALNFESDYLVRRG
                     YVQVIVDVRGTGSSEGRWNSFGAREQRDSAELVRWVASPTRPWSNGRVGLHGTSYGAI
                     NQLFTAAHQQPSVKAAFPIVPMSDAYRDITASGGQIDTSFIPSWLGLVTALGLLPPTY
                     SGSDPGRTLEVMNDHAEGAQSFQAQTVLDAMQGGSNAYDGAFYRTRSPIDVIDRVRIP
                     TFIMGGWFDLFQRGEPLLYQRLRANGVPVRLVMGPWYHITAGNGLPADGVPPANELEL
                     RWFDHYVRGVADPGLANTPSVTYFRNGEGHYHFAPSWPPPGVGYRQLYLSGPAEPGSA
                     GTLAAAPPAKQSPDTLPWQPLSGVCTRSTVQWTAGAGSGSLCESDDEFNDATGLAYDL
                     ALPRGLTVAGPIAVRLDVGTNGHDSLLAVRVEDVDPNGRATQLTAGWNTISLRALDRS
                     MSTFAGGLMVRPYHPFTKTSELPVVDGKAYAVWIEIFPTSARFAPGHSLRISIQPSDA
                     PHLSPTEPQLERSVGGVLSLYHDAAHHSEVILPVQG"
     gene            complement(2055..2837)
                     /locus_tag="VEO00_02465"
     CDS             complement(2055..2837)
                     /locus_tag="VEO00_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYU56899.1"
                     /translation="MCYDPAARPPLPPISGGAGSAEGERIELEAADGNRFAAYSVRHS
                     DRGGAGMVVLPDVRGLHPFYEELALRFADAGVHATALDYFGRTAGVAERDDDFEYMPH
                     VQATTPGGIAADVAAAAAHLRSPEGGAAERLFTVGFCFGGRNSFNQAARDHDLAGVIG
                     FYGFVAERDEHDRDAPTRLAAGYRCPVLGLFAGADRNITHEHVRRFDEALGGAGVTHH
                     VEIYDGAPHSFFDRAAEEHAEASADAWNRMLGFVRTGDPGAA"
     gene            2966..4900
                     /locus_tag="VEO00_02470"
     CDS             2966..4900
                     /locus_tag="VEO00_02470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYU56900.1"
                     /translation="MVPEDIGELTWVADPRVSPDGRQVAYVVMRVDREANEYRSAIWV
                     SAADGSAEPRQLTAGAKRDGSPRWSPDGAQLAFVSNRDGKVGQLYVIPMDGGEPRKLT
                     SAKEDVEEPAWSPDGSRIAYSARVPDPANEEEDERKRAPRRFTRLQFKLDNVGWTGDR
                     RQHLFVVAADGSAEPEQLTDGDFEDDGVCWSPDGTRLAFASAREEHWDTLPVRDVYAI
                     DASGGEAERLTGGDGTAERPSWSPDGRRIAHLVNPGVFDDPRHTQVAVLDVATGERTV
                     LTESLDRNCAPYPPAREPLWDGGDVLFAVEDHGATHLYRVPGDGSGKPELVVGGDRGL
                     TGWDAAGGTIAHTLSTPTEVPELFVGERRLTEVGRAFADAREIVDQERFTATSADGTE
                     VDAWIMRPAGFEEGRRYPTVLNVHGGPFAQYGMRFFDEFQVQAGAGYVVVFCNPRGSS
                     GQTEEWGRAIRGPVEGGPGWGTRDYEDVMAVVDEAVRRFDFVDGDRIGVMGGSYGGYM
                     TSWIVGHTDRFAAAISERSCNNFLLEGGSADIGWMFKGELGAFWFEDPEAYLKISPST
                     YATNITTPLLILHSENDLRCPVGHAEDLFTILRLLRREVELVRFPAESHELSRSGSPA
                     HRVQRFEVILDWLERHLGNA"
     gene            4960..5220
                     /locus_tag="VEO00_02475"
     CDS             4960..5220
                     /locus_tag="VEO00_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56901.1"
                     /translation="MTESTYRFEGTQVRHGGSSWRWMARIELGVAERTRLRLQLDPVV
                     GAPAVALRAPSRRIRRPPHHRGLTARERVSRPGDPRTRDRQA"
     gene            5264..5632
                     /locus_tag="VEO00_02480"
     CDS             5264..5632
                     /locus_tag="VEO00_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HYU56902.1"
                     /translation="MDWRLELVQVPVSDVDRAKAFYTEKVGFHLDHDHQVTDELRFVQ
                     LTPPGSACSIALGTGLGDMPPGSVKGLQLVVPDIHAARAELLERGLEVGEVQVFPWGS
                     FVFFSDPDGNAWSVQQIPPR"
     gene            complement(5685..6476)
                     /locus_tag="VEO00_02485"
     CDS             complement(5685..6476)
                     /locus_tag="VEO00_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HYU56903.1"
                     /translation="MTDQEQKGSAFRALHDGEPFVIPNPWDAGSARVLAALGFQALAS
                     TSSGFAFTLGRLDGHVSLDEVVEHARMLDRATDLPVSVDLENGYGPDPASAALAITRV
                     AGAGAVGGSIEDYDPGGRLYDLAHAAERIAAAAEAARALEFPFTLTARAENHIRGHPD
                     LDDTIARLRAYEAAGADVLFAPGLRSREEIHAVCEAVTKPVNVLARPGMSFFEIADAG
                     AQRVSVGGGLTWIAVNAMAAAAGEIRDRGDFSVLGESPPLREWFT"
     gene            complement(6469..7416)
                     /locus_tag="VEO00_02490"
     CDS             complement(6469..7416)
                     /locus_tag="VEO00_02490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HYU56904.1"
                     /translation="MSIKPISQSAAAAPTMRAVRLHQPGGLEALRVEEIGTPRPGPGE
                     ALVRVHAAAITRDELGWPVDRLPAIPSYELSGVVVEVASPDDAGIANGEAVYALTGFD
                     RDGAAAEYAVVPSRLLAPKARTLGHVESAALPLAGLSAWQGLFVHGALRAGDRVLIHG
                     AAGGVGHLATQLARWRGAHVIGTTSGAVAETGLGAHEMVDRTTTRFEDAVEPVDLVFD
                     TAGGDALARSATVLRAGGRLVSVAEEPPPEVAERVAATYFVVEPDRDQLIELARLVDE
                     GAVRPAIDSVFELADARAAFERVMDSGKRGKVVLEVIHD"
     gene            7528..8007
                     /locus_tag="VEO00_02495"
     CDS             7528..8007
                     /locus_tag="VEO00_02495"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYU56905.1"
                     /translation="MAIGRLAAVPSATRPGSLVVSSYVEVWGRSGRELRPLDLERMTV
                     GTLESNDVVVDADGVSRVHAVFERFGDTWCVRDLGSRNGTFVNGGRIIGERALHSGDE
                     IVLGRLRLLFHGPARGKETAAIAQAPPLTQRERDVLLALCRLLTGDAFTRRTCGPDG"
     gene            complement(8646..9212)
                     /locus_tag="VEO00_02500"
     CDS             complement(8646..9212)
                     /locus_tag="VEO00_02500"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYU56906.1"
                     /translation="MPQSRVDVRGLQAPIRERYLAEPESPPIVITVRSAGGDLGDPLH
                     CAVAPDAASDVVWRSGAHAAVGGSGDVPCSADLLLGALVACQETTLRMVAANAGIELE
                     ELEVTAEGDWDPRGTLAMGKEFPVGLTGVRCHTRVVVRGDARGERAERFLRSAERFCV
                     VLNTLRDGVPVRSTFDLQPAPGDPASGI"
     gene            9312..11525
                     /locus_tag="VEO00_02505"
     CDS             9312..11525
                     /locus_tag="VEO00_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU56907.1"
                     /translation="MAEPHPCPRCGAGNEPGQSFCGSCGARLVLTCPACGATSPLGFR
                     FCGSCGAELGSKGDEPRPTGEERRVVTVLFADLVGFTSRAEQLDPEDVRAILRPYYSS
                     LREEIESFGGTVEKFIGDAVVGVFGAPVAHGDDPERAVRAALRVRDAVDEMNTRDPRM
                     NLQVRVAVNTGEAIVSLDARAEEGEGMVAGDVVNTASRLQTHAPEGSILVGEETFRST
                     RGTIEYEPVEPIVARGKQLPVPAWRVVAAAFPPGERGASTVPMVGRESELATLSGLWD
                     RVVADRQPHLATIVGLPGIGKSRLAQEFIDAVRGAGGRAIVGRSLPYGESGAYGAFAQ
                     QVKQIAGIYDGDPPEMAIGKLRTSAADVVGAESADEVSAHLSLMLGLEGAAPSEGAVA
                     DRHVLFFSARRFVEGLGRAAPTVLAFQDLHWADASLLDLIEILGSRLEETTVLLLVLA
                     RPELRDKRPAWGSSVQAALALPLEPLDLAHANELAERLLADAAGPVDPAAAEEIGRAA
                     EGNPLFIEELVASMAERSSSTGAGLPTSIRGIIAARLDAIPPAERSVLLDASVMGRIF
                     WSGGLSRLGQTADALPAIMDSLEGRDLIRREAVSRYQGEQQFRFKHALIRDVAYATLP
                     RARRREAHGEVAAFLESVHAERDSPAALAHHWQEAGDVERAIEYFVAAADQASRGWAK
                     EEAVSLFRRALSLVQQEDRERFRKLRLKLAVAEQMLYHVPDAQQLARDRTSEPPA"
     gene            complement(11532..11813)
                     /locus_tag="VEO00_02510"
     CDS             complement(11532..11813)
                     /locus_tag="VEO00_02510"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HYU56908.1"
                     /translation="MPRYVIERTYDVDEDELPNVATRSKRIAQEQFPTIVWEHSHVVV
                     DEDGTLKSFCIYEAPDIATVRRHAELLGNHVVDHIYEIGGDVTPADFPL"
     gene            complement(11987..12274)
                     /locus_tag="VEO00_02515"
     CDS             complement(11987..12274)
                     /locus_tag="VEO00_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HYU56909.1"
                     /translation="MSEPSESDVLYLTLEDVLALYAEVFSCTQREAAEQLRDSAGLES
                     ALARPAMYAQYPEADLAMQVAVLAHGIAEGQYFLEGNKRTALAACSQTVRS"
     gene            complement(12271..12552)
                     /locus_tag="VEO00_02520"
     CDS             complement(12271..12552)
                     /locus_tag="VEO00_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56910.1"
                     /translation="MDIVKIRRVGNSNVISLPKSLEGLGYVSGTSVVVEELDSGELRV
                     VPVSKVRELIREVGRRVVAENREALQILERHDSQGSSPAGRSRARRRIG"
     gene            13330..13764
                     /locus_tag="VEO00_02525"
     CDS             13330..13764
                     /locus_tag="VEO00_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HYU56911.1"
                     /translation="MSTEENKAIVRRWFEEVLNEKKLDQADDHVAQDYVDHGALPGQA
                     PGLEGAKRKWAMYAAAFPDLRTWSEDMVAERDEVAVRWMAEGTQQGELLGIPPTGKQA
                     RFGGMSIFRVAEGKIAAQWEEWDKLDLMRQLGVMPSAGEGKS"
     gene            13857..14162
                     /locus_tag="VEO00_02530"
     CDS             13857..14162
                     /locus_tag="VEO00_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HYU56912.1"
                     /translation="MSTEENKKVVLRWIEQAWEKKNVDVIDELHALDYVGHIVGTPGP
                     VRGREAFKQLFTTYLAAFDLQRTNEFLIAEGDRWWPTTPTASSTKVSSPVSRRRGRS"
BASE COUNT         2224 a         5056 c         5330 g         2138 t
ORIGIN      
        1 gttctccata ggccagaatt atagccacat ccgaagacga gatccgcgac ggtccacgga
       61 cgacatcaga ccctggttcg cgcaccactc ggctggagaa agctgacgtt ccaggtcagg
      121 agggccgtgg accagagggg agttgaacca acggttctct cctgttgttg attggtcatc
      181 cctggacggg aaggatcacc tcggagtggt gggcggcgtc gtggtacagg ctgaggacgc
      241 cgccgactga gcgttcgagc tgcggttcgg tcggggagag gtggggggcg tcgctgggct
      301 ggatgctgat gcgcagcgag tgcccggggg cgaaccgggc cgaggtgggg aagatctcga
      361 tccacacggc gtaggccttc ccgtcgacca ccggcagctc gctggtcttc gtgaaggggt
      421 ggtaggggcg gaccatgagg ccgccggcga acgtcgacat cgaacggtcc agggcccgga
      481 gcgagatcgt gttccagccc gcggtgagct gggtggcccg gccgttcgga tcgacgtcct
      541 ccacccggac cgcgaggagg ctgtcgtggc cgttcgttcc cacgtccagg cgcaccgcga
      601 tgggtccggc caccgtgagg cccctcggca gggcgaggtc gtaggccagg ccggtggcgt
      661 cgttgaactc gtcgtcactc tcgcacagcg agcccgatcc ggcccccgcg gtccactgca
      721 ccgtcgaacg cgtgcacact ccggacaggg gctgccacgg cagcgtgtcg ggtgactgct
      781 tcgccggcgg ggcggcagcg agcgtcccgg cgctccccgg ctcggcgggc cccgacaggt
      841 agagctgccg gtacccgacg cccggcggtg gccacgaggg cgcgaaatgg tagtggccct
      901 cgccgttgcg gaagtacgtc acggacggcg tgttcgcgag ccccgggtcc gcgacgccgc
      961 gcacgtagtg gtcgaaccat cgcagctcca actcgttggc gggcgggact ccgtcggcgg
     1021 gcaggccgtt gccggccgtg atgtggtacc agggccccat caccaggcgc accggcacgc
     1081 cgttcgccct cagccgctga tacagcaggg gctcgccccg ctggaacagg tcgaaccacc
     1141 cgcccatgat gaacgtgggg atgcggacgc ggtcgatgac gtcgatcggc gaccgcgtcc
     1201 ggtagaaggc gccgtcgtac gcgttggagc cgccctgcat cgcatcgagg acggtctgcg
     1261 cctggaacga ctgagcgccc tccgcgtggt cgttcatgac ctcgagcgtc cggcccggat
     1321 ccgagcccga ataggtcggc ggcagcagcc ccagggcggt caccaggccg agccacgacg
     1381 ggatgaagct cgtgtcgatc tgcccgccgg acgcggtgat gtcgcggtac gcgtccgaca
     1441 tcggcacgat cgggaacgct gccttcaccg agggctgctg gtgggccgcg gtgaacagct
     1501 ggttgatcgc gccataggag gtcccgtgca gccccacccg gccgttcgac cagggcctcg
     1561 tcggcgaggc cacccatcgc accagctcgg cgctgtcccg ttgctcccgg gccccgaacg
     1621 aattccagcg gccctcggac gaaccggttc cccggacgtc aacgatgacc tgcacgtacc
     1681 cccgccgcac caggtagtcg gactcgaagt tcagcgcacc gttcttgttg tacggggtct
     1741 gggtgagcac caccgggaac cgcccgggcg ccggcgtgcc gtcggccctc gcggggcgga
     1801 ggacgtcggc gtacaggacc accccatcgg acatccggat gggcacgtcg gcggtgcggg
     1861 ccacgccgta cgtggcgggc cgcggcgacc atcgggtcca ggacgaggcg gcgccggcgg
     1921 gggaggggac ggccaccgcg agggcggcga cgagcgaggc tgcggcgagg atgcgggctc
     1981 tcatcggcgg gctccactca agcggcgggc gccggttcgt ctcttacccg cttgctccgt
     2041 tcaggaaaac aacgtcaggc ggcgcccggg tcgccggttc gaacgaagcc gagcatccgg
     2101 ttccaggcgt ccgcggaggc ctcggcgtgc tcctcggcgg cccggtcgaa gaacgaatgt
     2161 ggcgcgccgt cgtagatctc gacgtggtgg gtgacccccg cgccgccgag cgcctcgtcg
     2221 aaccgccgga cgtgctcgtg cgtgatgttc cggtcggcac ccgcgaacag cccgagcacc
     2281 gggcagcggt acccggccgc cagccgggtc ggcgcgtccc ggtcgtgctc atcccgctcc
     2341 gcgacgaacc cgtagaaccc gatcacgccg gccaggtcgt ggtcgcgggc cgcctggttg
     2401 aacgagttcc gcccgccgaa gcagaacccc accgtgaaca gccgttcggc ggcgccgccc
     2461 tccggcgagc ggaggtgtgc ggccgccgcg gcgacgtcgg ccgcgatgcc cccgggcgtg
     2521 gtggcctgca cgtgcggcat gtactcgaag tcgtcgtcgc gctcggcgac gcccgcggtc
     2581 cgcccgaagt agtcgagcgc ggtggcgtgc accccggcgt cggcgaaccg gagcgccagc
     2641 tcctcgtaga aggggtggag gccccggacg tccgggagga cgaccatccc cgcaccgccg
     2701 cggtcggagt gccgcaccga gtaggcggcg aaccggttcc cgtcggcggc ctccagctcg
     2761 atgcgctcac cctcggcgga tccggctcct cccgagatcg gcggcaacgg cggtctggcg
     2821 gcggggtcgt agcacatgag ggcctcctcg attcgctcgc ggatccgcgg gcggaagcct
     2881 atcgccccga ccggggcctt ccggcgtgcc aagattggaa cgctgggagg gaatcggacc
     2941 tgagggaggg acgaacgtgg ctgggatggt tccggaggac atcggcgagc tgacgtgggt
     3001 ggccgacccg cgggtgtcgc cggacggtcg gcaggtggcg tacgtcgtca tgcgggtcga
     3061 ccgtgaggcc aacgagtacc ggagtgcgat ctgggtctcg gcggcggatg gctccgccga
     3121 gccccggcag ctcaccgcgg gggcgaagcg cgacggctcg ccgcgatggt cgcccgacgg
     3181 cgcgcagttg gcgttcgtgt cgaaccgcga cggcaaggtg ggccagctgt acgtgatccc
     3241 gatggacggc ggcgagccgc gcaagctcac gtcggccaag gaggacgtcg aggaaccggc
     3301 gtggtcgccg gacgggtcgc ggatcgcgta ttcggcccgg gtcccggatc ccgccaacga
     3361 ggaggaggac gagcgcaagc gcgcgccgcg gcggttcacc aggctccagt tcaagctcga
     3421 caacgtcggc tggaccgggg accggcgcca gcacctgttc gtcgtggccg cggacgggag
     3481 cgccgaaccc gagcagctca ccgacggcga cttcgaggac gacggtgtgt gctggtcgcc
     3541 cgacggcacc cggctggcgt tcgcctcggc ccgggaggag cactgggaca ccctgccggt
     3601 gcgggacgtc tacgcgatcg acgcgtccgg cggcgaggcg gagcggctga ccggcggcga
     3661 cggcacggcc gagcggccgt cgtggtcccc ggatgggcgg cgcatcgcgc acctggtcaa
     3721 ccccggcgtg ttcgacgacc cccgccacac gcaggtggcg gtgctcgacg tggcgaccgg
     3781 cgagcgcacg gttctcaccg agtccctgga ccggaactgc gcgccgtacc cgccggcccg
     3841 ggaacccctg tgggacggcg gcgacgtgct gttcgcggtg gaggaccacg gcgccacgca
     3901 tctgtaccgg gtcccggggg acggctcggg gaagcccgag ctcgtggtgg gtggcgaccg
     3961 ggggctcacc gggtgggacg ccgccggcgg gaccatcgcc cacacactgt cgacgcccac
     4021 cgaggtcccg gagctgttcg tgggggagcg gcggctgacc gaggtggggc gggcgttcgc
     4081 cgatgcccgg gagatcgtgg accaggagcg gttcacggcg acctcggccg acggcacgga
     4141 ggtcgacgcg tggatcatgc gccccgccgg cttcgaggag ggccggcggt atccgacggt
     4201 gctgaacgtc cacggggggc cgttcgccca gtacggcatg cggttcttcg acgagttcca
     4261 ggtccaggcc ggcgcggggt acgtggtcgt gttctgcaat ccgcgaggct cgtccggtca
     4321 gacggaggaa tggggccggg ccatccgggg gccggtggag ggggggcccg ggtgggggac
     4381 ccgcgactac gaggacgtca tggccgtcgt ggacgaggcc gtgcggcggt tcgacttcgt
     4441 ggacggcgac cgcatcggcg tcatgggcgg ctcgtacggc ggctacatga cgtcgtggat
     4501 cgtggggcac acggaccggt tcgccgcggc catcagcgaa cggtcctgca acaacttcct
     4561 gctggagggg ggaagcgccg acatcggctg gatgttcaag ggcgagctgg gggcgttctg
     4621 gttcgaggat cccgaggcct atttgaagat ctccccgtcg acctacgcga cgaacatcac
     4681 cacgccgctg ctgatcctgc actcggagaa cgacctgcgg tgcccagtgg gccacgccga
     4741 ggacctgttc acgatcctgc gcctgctccg ccgcgaggtg gagctggtcc ggttcccggc
     4801 cgagagccac gagctgagcc gctcgggttc cccggcccac cgcgtccagc gcttcgaggt
     4861 catcctggac tggctcgaac gccacctggg caacgcgtag ccgccttccc gaccaagagg
     4921 gccgcctccg agctcgacca ggccgccaac gcgtggaacg tgaccgagtc gacctaccgg
     4981 ttcgagggca cgcaggtgcg ccacgggggc tcgtcgtggc ggtggatggc ccggatcgag
     5041 ctgggagtgg cagagcggac acgtctccgg ctccaactgg atcctgtggt gggagcgccg
     5101 gcggtggccc tccgagcccc gtcccgtcga atccgccgcc cgccgcacca ccgcggcctg
     5161 accgcgaggg agcgggtcag ccggcccggc gacccccgga cccgtgaccg acaggcctga
     5221 cggcaccgga tctcgatgga gccctgctac agtgccgccc cacatggact ggcgactcga
     5281 gctggtgcag gtccccgtct ccgacgtgga ccgggcgaag gccttctaca cagagaaggt
     5341 cgggttccac ctggaccacg accaccaggt caccgacgag ctccgattcg tccagctgac
     5401 gcctcccggt tcggcctgct ccatcgccct gggaacgggg ctcggcgaca tgccgcccgg
     5461 atccgtcaag ggcctccagc tggtggtccc ggacatccac gcagcgcgcg ccgagctcct
     5521 ggaacgcggg ctggaggtcg gcgaggtcca ggtgttcccc tggggctcgt tcgtcttctt
     5581 cagcgacccg gacggcaacg cctggtccgt ccagcagatc ccccctcgct gacgaacccc
     5641 ggcgagagtg tcgaagccgc gcgccggacg atgtcacgcg atggtcacgt gaaccattcg
     5701 cgcagcgggg ggctctcgcc gagcaccgag aaatccccgc gatcgcggat ctcgccggcg
     5761 gcggcggcca tggcgttgac ggcgatccac gtcaggccgc ccccgacgct cacccgttgc
     5821 gccccggcat ccgcgatctc gaagaacgac atccccggcc gcgcgagcac gttcaccggc
     5881 ttcgtgacgg cctcgcagac ggcgtggatc tcctccctgc tccgcaggcc gggggcgaag
     5941 agcacgtcgg ctcccgccgc ctcgtacgcc cggaggcggg cgatcgtgtc gtcgaggtcg
     6001 gggtggcccc ggatgtggtt ctcggcccgt gccgtcagcg tgaacgggaa ctccagcgcc
     6061 cgcgcggcct cggcggcggc ggcgatccgc tcggcggcgt gcgcgaggtc gtagaggcgg
     6121 ccgcccgggt cgtagtcctc gatcgatccg ccgacggccc cggccccggc gaccctggtg
     6181 atcgccagcg ccgcgctcgc ggggtccggg ccgtagccgt tctccaggtc caccgagacg
     6241 gggaggtcgg tggcgcgatc gagcatccgc gcgtgttcga cgacctcgtc caggctcacg
     6301 tggccgtcga ggcgcccgag cgtgaacgcg aaccccgagc tggtgctggc cagcgcctgg
     6361 aagccgagcg cggccaggac gcgcgccgac cccgcgtccc acggattggg gatcacgaac
     6421 ggctcgccat cgtggagcgc ccggaacgcc gaccccttct gctcctgatc agtcatgaat
     6481 cacctccagc acgaccttgc cacgctttcc ggaatccatc acccgctcga acgccgcgcg
     6541 ggcgtcggcc agctcgaaca ccgaatcgat cgccggccgc actgcgccct cgtcaacgag
     6601 ccgggcgagc tcgatgagct ggtcgcgatc cggctccacg acgaagtagg tggcggcgac
     6661 ccgttcggcg acctctggcg gaggctcctc ggcgaccgac accagcctgc cgcccgcgcg
     6721 gaggacggtc gccgagcgcg cgagcgcgtc gcctcccgcc gtgtcgaaga cgaggtccac
     6781 gggctccacg gcgtcctcga acctcgtggt cgtgcgatcg accatctcgt gggccccgag
     6841 gccggtctcg gctacggctc ccgaggtggt gccgatgacg tgcgccccgc gccatcgcgc
     6901 gagctgcgtg gcgaggtgtc cgaccccgcc ggccgcgccg tggatcagca cgcgatcacc
     6961 cgcccggagc gcgccgtgga cgaacagccc ctgccacgcg ctgagcccgg ccagcgggag
     7021 cgcggcgctc tccacgtggc cgagcgtccg ggccttcggc gcgagcaatc tggacgggac
     7081 gacggcgtac tcggcggctg cgccgtcccg gtcgaatccc gtgagcgcgt acacggcctc
     7141 gccgttcgcg atgccggcgt cgtccggcga cgcgacctcg acgacgaccc cggatagctc
     7201 gtatgacggg atcgcgggaa ggcggtccac cggccacccg agctcgtcgc gagtgatggc
     7261 agcggcgtgg acccggacga gggcctcccc cggccccggc cgcggggtcc cgatctcctc
     7321 gaccctcaac gcctcgaggc cgccgggctg gtggaggcgc accgcgcgca tggtgggagc
     7381 ggcggccgcc gactgggaaa tgggcttgat gctcactggg accatctcct cctctggtga
     7441 atgactggat gcttgcaaca cgtttgcatg gaatccggac gtcgacgctg actccgtgcg
     7501 cggcgagggc ttcgctcgaa ctcgaccatg gcaatcggcc gccttgcagc ggtaccctct
     7561 gcaacccgac ctgggagtct cgtggtgagc agctacgtcg aggtgtgggg ccgaagcggg
     7621 cgggagcttc gaccgctgga cttggagcga atgaccgtgg gaaccctcga gtcgaacgac
     7681 gtggtcgtcg acgccgacgg ggtctcccgg gtgcatgctg tgttcgagcg attcggcgat
     7741 acctggtgtg tccgcgacct cggctctcgc aacggcactt tcgtgaacgg aggaaggatc
     7801 atcggcgagc gagccctgca ctcgggcgat gagatcgtcc tcggacgact tcgcctgctg
     7861 ttccacggcc cggcccgggg gaaggagacg gccgccatcg cgcaggcgcc gccactgacg
     7921 cagcgcgagc gcgatgtcct cctggccctg tgtcgcctgc tgacgggcga tgcgttcacc
     7981 cgccgaacgt gcgggcctga cggttgagga gaagctcgtc gattccctcg catcggacac
     8041 gaccggtcag ccgggtggcc tgcccctcct ctcgacggcg ctgctggagc tgtggaccca
     8101 cctgggtgag tgaccggccc ggagtgcgcg acggagcctc gaaacgtagg agccgaccgt
     8161 cttctccgcg gttcgaggtg ggtgctcacc ccacagctcc tcgatgaggt cgtcgacgga
     8221 cacgacacgt cccccacgag cgagcaggaa ggcgaggatc gtgcgttcct tcgatccggc
     8281 gatcggcaac ggatccccgg tttccgtcac gacctcgagg gaccccagga cgaggaaacg
     8341 catcctccgg aggctagcgg cctcgaccgt gggtggctag ccccagctac atggcgtgag
     8401 cttcctgttc ctgcggcggc atcagactcg ctctcagggg gccaagcgta cgaactcaag
     8461 accgttccgc gagctccttc tgggagagct tccgggcggt ccggtgggac cggatcatcc
     8521 gtgccagctc ctcgagttgg cgccgccggt gcttctcgtc tcgctgggca cgggaccgcg
     8581 ctggtagttc acggcgaagg gtgtcctcat cgagccaccg ccacgatcct cggcggcgcc
     8641 gacgctcaga tgccggacgc ggggtctccg ggcgccggct ggaggtcgaa cgtggagcgc
     8701 acgggcaccc cgtcgcggag cgtgttgagg accacgcaga accgctcggc gcttcgcagg
     8761 aaccgctccg cccgctcccc gcgggcgtcg ccccgcacga caactcgcgt gtggcaccgc
     8821 acaccggtca gtcccactgg gaactccttc cccatcgcga gggttccccg cgggtcccag
     8881 tccccctccg ctgtgacctc gagctcctcg agctcgatgc cggcgttggc cgccaccatc
     8941 cgaagggtcg tctcctgaca ggccacgagg gcaccgagga ggagatcggc cgagcacggc
     9001 acgtcgcccg agccccccac tgccgcatgg gcgccggacc gccacaccac gtccgaggcg
     9061 gcgtcgggtg cgacggcgca gtggagcggg tcgccgaggt cccctccggc gctccgaacc
     9121 gtgatgacga ttggcgggga ctccggttcg gccaggtagc gctcccggat cggcgcctgg
     9181 agcccgcgga cgtcgactcg ggactgcggc atcgccccat catcccccgg gggggacggg
     9241 ggcggcaagc cggggcttcc ggcgggcggt gccaccgccc atactgggca ccccgaggcg
     9301 aaaggcgagc ggtggccgaa ccacatccgt gcccgcgttg cggcgcaggg aacgagccgg
     9361 gccagagctt ctgcggctcc tgcggcgccc ggctcgtcct tacgtgcccc gcgtgcggcg
     9421 ccaccagccc gctcgggttc cggttctgcg gaagctgcgg cgccgagctc gggtcgaagg
     9481 gggacgaacc gcgtcccacc ggcgaggagc gacgcgtcgt caccgtactg tttgcggact
     9541 tggtggggtt cacgtcgcgg gcggagcagc tggaccccga ggacgtgcgc gccatcctcc
     9601 ggccgtacta ctcgtcgctt cgcgaggaga tcgagtcgtt cggcggaacg gtggagaagt
     9661 tcatcggcga cgccgtggtg ggcgtgttcg gggcaccggt cgcacacggc gacgaccccg
     9721 agcgcgcggt gcgggcggcc ctgcgggtcc gcgacgccgt cgacgagatg aacacccgcg
     9781 atccccggat gaacctccag gtccgcgtcg ccgtgaacac cggcgaggcc atcgtcagcc
     9841 tcgatgcccg cgctgaggag ggcgagggca tggtggcggg cgacgtcgtg aacacggcgt
     9901 cgcggctgca gacccacgcg cccgagggct cgatcctggt gggcgaggag accttccgca
     9961 gcacccgggg caccatcgag tacgagcccg tcgagccgat cgtggccagg ggcaagcagc
    10021 tcccggtgcc cgcgtggcgg gtggtcgcgg cggcgttccc gcccggtgaa cgtggcgcct
    10081 ccacggtgcc gatggtgggg cgcgagagcg agctcgccac gttgtcgggt ctctgggatc
    10141 gcgtcgtggc ggaccggcag ccacacctgg ccacgatcgt ggggctcccc gggatcggaa
    10201 aaagcaggct ggcccaggag ttcatcgacg cggtccgggg ggccggcggg cgcgccatcg
    10261 tcgggcgatc gctgccgtac ggggagagcg gcgcgtacgg cgccttcgcg cagcaagtga
    10321 agcagatcgc cgggatctac gatggcgatc cacccgaaat ggccatcggc aagctccgga
    10381 cgtcggcggc cgacgtcgtc ggggcggaga gcgcggacga ggtctcggcg cacctgtcgc
    10441 tgatgctcgg gctggaggga gcggccccct ccgagggcgc ggtcgccgat cgccacgtcc
    10501 tcttcttctc cgcgcggcga ttcgtggagg ggctgggccg cgccgcgccg accgtgctcg
    10561 cgttccagga cctccactgg gccgacgcga gcctcctcga tctcatcgag attctcggct
    10621 cgcggctcga ggagactacg gtgctgctgc tcgtgctggc ccgccccgaa ctgcgggaca
    10681 aacgcccggc ctgggggtcc tcggtgcagg cggcgctcgc ccttccgctc gaaccgctcg
    10741 acctcgcgca cgcgaacgag ctcgcggagc gcctcctcgc ggacgccgcc ggccctgtcg
    10801 accccgccgc ggccgaggag atcggcaggg cggcggaggg caacccgctc ttcatcgagg
    10861 agctcgtggc gtcgatggcc gagcgatcct cgtcgacggg ggcggggctg cccaccagca
    10921 tccgcgggat catcgccgcc cgcctcgacg ccatcccccc cgccgagcgc tcggtgctgc
    10981 tcgacgcgtc ggtgatgggc cgaatcttct ggagcggcgg gctcagccgg ctgggacaga
    11041 ccgccgacgc cctgccggcg atcatggact cgctcgaggg ccgcgacctg atccgccggg
    11101 aggccgtctc ccggtaccag ggcgagcagc agttccggtt caagcacgcg ctgatccgcg
    11161 acgtcgctta cgccacgctt ccccgcgcca ggcggcggga ggcccatggc gaggtggcag
    11221 cgttcctgga gtcggtgcac gccgagcggg actcgcccgc cgcgctggcc caccactggc
    11281 aggaggccgg cgacgtagaa cgcgcgatcg agtacttcgt ggccgctgcg gaccaggcca
    11341 gccgggggtg ggccaaggag gaggcggtgt cactcttccg gcgtgccctc tcgctcgtgc
    11401 agcaggagga ccgcgaacgg ttccgcaagc tccggctcaa gctggcagtt gcggagcaga
    11461 tgctgtacca cgtgcccgac gcgcagcaac tcgcgaggga ccggacctct gagccgcccg
    11521 cgtagcgtcg gttacagcgg aaagtccgcc ggggtgacgt ctccgccgat ctcgtagatg
    11581 tggtcgacca catgattgcc gaggagctcc gcgtgccgcc ggaccgtcgc gatgtccggc
    11641 gcctcgtaga tgcagaacga cttcagcgtg ccgtcctcgt ccacgaccac gtggctgtgc
    11701 tcccacacga tggttgggaa ctgctcctgg gcgatccgct tcgaccgggt cgccacgttc
    11761 ggaagctcgt cctcgtcgac gtcgtaggtg cgttcgatca cgtacctggg catctcattg
    11821 tctcccctcg tccccgtcga gggattgtct cacccggcgc ggccgcgtga ccggcggctc
    11881 agatggacgc ggcgtccagc gcggggccat cggaacaccg aaagactcgt gaagcagaag
    11941 cggtcttcgg cgcccgagga aggctctcta gcccgtgcgg cccacgctac gaccggacag
    12001 tctgcgagca ggctgccagg gccgtccgct tgttgccctc caggaagtac tggccctcgg
    12061 cgatgccatg ggcgaggacg gcgacctgca tcgccagatc cgcctccggg tactgggcgt
    12121 acatcgccgg tcgggccagg gccgattcca gacctgccga gtcacgaagc tgctcggccg
    12181 cttcccgctg ggtgcaggag aagacctcgg cgtagagcgc cagcacgtcc tccagcgtga
    12241 ggtagaggac gtcgctctcg ctgggctcgc tcacccgatg cggcggcggg ccctgctccg
    12301 tccggcgggg gaggaccctt ggctgtcgtg ccgctcaagg atctggagag cctcgcggtt
    12361 ctcggcaaca acgcggcgcc cgacctcccg gatcagttct cggaccttgc tcaccggaac
    12421 gacccgcagc tcaccagagt ccagctcttc gacgacgacg gacgtgccgc tgacgtaccc
    12481 cagcccctcc agggacttgg gcagcgagat cacgttgctg ttcccgaccc ggcgaatctt
    12541 cacgatgtcc atcgaagggc agtatataca gggatatccg aacggtcaac ggctcctcaa
    12601 acgccgagcc cgggtcggac cgtcgtaagc ctccgaaggg ctggcggagg cgaaggtgga
    12661 ttcctatccg gcgtgatgga tgggcggttt gctggacaga tacgggcgag cgcgcgagca
    12721 gaccggccgt ggcgcgcccc accgcctctt gaggtcgccg gcgtcgacac gcccggcata
    12781 cgctcaaggc gtggacgacg tcgaacggga gcgacgggaa cggatgctgg caccggagga
    12841 gatgggtgac ggcgagacct ccgaacgtgc gatcctcggc ctgtcgcaga tcgaccttca
    12901 cgcaagggag cacgagacag cccactggcc tcgtcgccgg agcgctcgcc gccggctgtc
    12961 agatcgtcgg gcgaagtcag cacggtcccg gatccaccag cttttcaatt catggtcgcc
    13021 gggagcgatg gttggacgag ggcgcgggcg cgttcccgtc actgccaatt gctcggttga
    13081 cgccagccat cgtcgtacgg ttctgatgct gctgacctgg gccgctcgcg actctgagtc
    13141 agactcggct gctgaccact caaagggcac cagccttgct gaggtgccgt cctcgtcggt
    13201 cgacgccagc cacctgggcg tgctcacctg ggggcaaggc ggtccgcggt ctccgcccga
    13261 tggtggcctg accggccata ccgaaggcgc cgtcgaaccg cggagccgga gtaggaggag
    13321 accatctcca tgtccaccga agagaacaag gcgatcgtcc ggcgctggtt tgaggaggtc
    13381 ctcaacgaga agaagttgga tcaggccgat gatcacgtcg ctcaggacta cgtggaccac
    13441 ggtgccctgc ccgggcaagc gcccgggttg gaaggagcca agcgcaagtg ggcgatgtac
    13501 gctgccgcat ttcctgatct gcgcacctgg agcgaggaca tggtcgcaga aagggacgaa
    13561 gttgcggtgc gctggatggc cgaggggacc caacaggggg agttgctcgg catcccgccc
    13621 accgggaaac aggcccgatt cggcggcatg agcatcttcc gcgtggccga ggggaagatc
    13681 gcggcgcaat gggaggagtg ggacaagctg gatctgatgc gccagctcgg cgtgatgccc
    13741 tcggcagggg agggcaaatc ctaaggccag gccaggcgac agcgacgcgc gcgctgggcg
    13801 actgcgggac cgaccactgg tactggcccg cgcttcgggg agaggagagt tacgcaatgt
    13861 ccaccgaaga gaacaagaag gtcgttctgc gctggataga acaggcgtgg gagaagaaga
    13921 acgtggacgt catcgatgag ctgcacgccc ttgactacgt cggccatatc gtcggtaccc
    13981 ctggaccggt ccgaggccgc gaggcattca aacagctatt caccacctat ctcgccgcct
    14041 tcgacttgca gcgcacaaac gagttcctca tcgcggaggg cgacaggtgg tggcctacga
    14101 cacctaccgc gtcaagcaca aaggtgagtt cgccggtatc ccgccgacgg ggaaggagct
    14161 gagcgctacc gggattgaca tttacaggat cgtggacggc aagatcgtgg agcagtggta
    14221 cgaaatggac ttcacgggct tgctgcaaca actgcggtag ggtctgaatc actggcggga
    14281 accggctcct cctgatcagc cggcgcttgg cgacccgggc gaccatcgga tcgctcgggc
    14341 gggacgccaa aagcgctggt agcggaggcg aaccggacac tgtacgcacc ccgtccaagc
    14401 gaaagacggg ttgaatggcg tcgcggccct ccacgtcgat ccgctcgacc agggcgccga
    14461 ggagcccttt ggttacctgg ggctcgccat ttgtgatcgc cccacgcaac gacccggctg
    14521 tctcagccac aaggctctcg ggcggagcgt ccggcgcaag gttgagctcg ctctgtaggc
    14581 gctcccgaga cgccggcgga tacgcgcgcc aacctctggc gaacatccgc gtgagctaca
    14641 cgcgcggccc cgatcggctt gggatgatgc tcgccgtgag gcgcgtccaa cgccgagcgc
    14701 acgcctgggc cgtgatggtt gggcggaagt cactggctgc tccctgtt
//
LOCUS       DASPWO010000113         7188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1204486, whole genome shotgun sequence.
ACCESSION   DASPWO010000113 DASPWO010000000
VERSION     DASPWO010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204486"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="VEO00_02535"
     CDS             complement(<1..1703)
                     /locus_tag="VEO00_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYU56913.1"
                     /translation="MAELRAGAVLGNYRVDWLIGRGGMGTVYLAQHVRMATRKAALKV
                     LSSELAEDPEFRERFIRESDLAGSLEHPNIIPVYDAGEADGVLYSAMRYVRGTDLGAL
                     IDLEGKLSPARTFRVMAAVGRALDFAHAHGLLHRDVKPGNVMIEPSRTAGGRERAFLT
                     DFGLVKRLLTPTKLTRTGHLVGTLDYTAPEVFRGEELDGRADIYSLGCVLFECLTGSV
                     PFPETTDAGVMYGHLQNEPPPVTSVRPELPTGLDVVVARAMAKDPEQRFATCAELLEA
                     TKQALMPSGIQASGEEPVSSTAPTERVDQAPLARTKIAEPPPLRVLPPPPRTEPSETH
                     QVRPRRRRVRTALALVTAIAVLGAAALAFVSSRHGGRHAAAPNTVIATIPVGQRPVAA
                     TFGAGSLWVVNSVDGTVSRIDPATNSVVATIQVGSNPQSVAATASRVWVANAGDGTVS
                     KIDTATNRVIGSIEVGRAPEGVVLTKSEAWVANAASNTVSDIERGTFSALRTFRVGAG
                     PVAITSGGGAVWVANVADDSVSRIDPLSFRRPTTIPVPAGAVAVAFGGGALWVANGGE
                     R"
     gene            1818..2972
                     /locus_tag="VEO00_02540"
     CDS             1818..2972
                     /locus_tag="VEO00_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HYU56914.1"
                     /translation="MRFLHTGDWHVGKAIRGRSRMDEFERALDQVVGIAVDERVDAVL
                     VAGDLYEHRSAAPDADRMVFESLLRLHSNGIPVVAIPGNHDSALRFEAFAGLLKAVDV
                     DVASRVLPPEQGGVIALGSRDGTQTLRVACVPFVPERRFGSAVELFQSTEAWFQSYAE
                     GMGNLMDAMAAGFEPDAVNVVLAHLFAAGAKLGGGEREVTVGLDYAVPASRLPANASY
                     LALGHIHRPQRIPGSRAPARYAGSLIQLDFGETEQDKSVAIVEADPGRPAKVREVSVT
                     AGRRLVDVEGTLDQVHIRAADLGDAFLRVFVDTDGPVPGIADQVRDLLPNAVDVHLRY
                     ERDEGAAAGPPLSSLKPREQFEAYVRAEHGADPDPALMAAFDEVAEAEEG"
     gene            2987..5557
                     /locus_tag="VEO00_02545"
     CDS             2987..5557
                     /locus_tag="VEO00_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HYU56915.1"
                     /translation="MKLEIKGFTSFRDEQVVDFDGLDLFAIVGPTGSGKSSVLDAMTY
                     ALFGQVDRVDGSETPMREMVSQGQPRMAVALEFEVGADRYRVTRSMPRSGATRILLQR
                     WEGGEWRQAGEGADRVRDANRKLQDLIGLDFDGFTRSVLLPQGKFSAFMSGDAKERRD
                     ILTDLLGLSLFDRMGKRARQVAKESKEKADTVEELIESQFAGATPEALDGARRAAQSV
                     AERRDALTEAGERVRGIVDRWRAVAKDVADLRSCASEAAGLARTAARHAETLEALAAA
                     AAKADRGVTDAETAAAAAGEARDAAGSAREAAEREWGTAADLARAHAEAVRLALARGE
                     LRRRRGVLEQAERAVPESAAAVEEAERALDGANAESERLRSDHGAAAAALEDVRHADQ
                     VSAMVSNLAVGDPCPVCGEPLAKLPDAPGAKAMKAAERGEAGSRTAAEAAARAAGEAE
                     RALDRARAAMQAAERERDAAAGEAERCGIEVAGLEAAIGRLLPEAVDDPAAELASRSA
                     RLEGLVRAERDAEATATAADRAAQEARGARERIRGDAGASRAGLDGISVASLAERASA
                     IAPDLEAPALPDLPASEDIPALAVSARLRADALARYGGSVEGAATDRAASEGAFVDEA
                     RGAVTGLGIEPGAPDGLDGLAADVDRAVRDAVAEASARQGDAERLELDLARVAELREQ
                     VRTLRARAAVFRQLAQELQADRLIAFLQAEALRLLAVDGARRLSALSSGRYALEYDHD
                     EFLVLDRWNGDETRSVRTLSGGETFLASLALALALAEQVSSLAVTEHASLDSLFLDEG
                     FGTLDPETLDVVVEAIEQLGGDGRMVGVITHVRDLADRLPARLEVTKSPRGSTVSRAV
                     "
     gene            complement(5567..7000)
                     /locus_tag="VEO00_02550"
     CDS             complement(5567..7000)
                     /locus_tag="VEO00_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYU56916.1"
                     /translation="MAADELDAVVVGAGPNGLAAAVHLARSGRSVLVLERSRAVGGGS
                     RSGPLTIPGFVHDICSSVHPFAVASPFFRGLPLADHGLEFVHPDVPLAHPLDDGTAVV
                     LDRSMTETASGLGADGRAWRRLFEPFVDRFDDLAEQVMGPLRPPRHPVLLGRFGLHGV
                     RSARAVAEGSFDGERARALFAGLAAHSVLPLDEQLTAAFGLLLGAAAHAVGWPVVAGG
                     SQGLPDALAGLLRSLGGEVRTGTPGRSMGDLPPARQYLFDLTPSALAGIAGDRLPASY
                     RDRLGRFRHGPGVFKVDLALDGPLPWRASECAGAGTVHLGGTLEEIAASESDVGRGEH
                     PERPFVLLVQATRFDGTRAPEGRHTVWAYCHVPNGSTVDMTERIEDQIARFAPGFRAL
                     VSGRHTMGPADLERYNPNYVGGDIAGGAHGGFQLFARPAPSLDPYSTPADGIYLCSAS
                     TPPGAGVHGMCGYYAAEAALRRNDRVA"
BASE COUNT          990 a         2537 c         2762 g          899 t
ORIGIN      
        1 tgccgttcgc cgccgttcgc cacccacagt gccccgccgc cgaaggccac cgccaccgcg
       61 ccggcgggca ccgggatggt cgtcgggcgg cggaacgaga gcggatcgat cctggagacc
      121 gaatcgtcgg cgacgttcgc cacccacacg gccccgccgc cggacgtgat ggccacgggg
      181 ccggctccca ctcggaacgt ccgcagcgcg ctgaaagtgc ctcgttcgat gtcggagacg
      241 gtgttgctgg cggcgttcgc gacccacgcc tccgacttgg tcaagaccac gccctcgggg
      301 gcccgtccga cttcgatgga cccgatcact cggtttgtcg cggtgtcgat cttcgacacg
      361 gtcccgtcgc cggcattcgc gacccacacg cggctggctg tggccgcgac cgactgcggg
      421 ttcgacccga cctggatcgt cgcaacgacg ctgttcgtgg cgggatcgat ccggctcacc
      481 gtgccgtcga cggagttgac gacccacagc gaaccggccc cgaacgtcgc cgcgacgggg
      541 cgctggccga ccgggatcgt ggcgatgacc gtattcggtg cggccgcgtg tcggccaccg
      601 tgtcgtgacg agacgaacgc gagggccgcc gcaccgagaa ccgcgatcgc ggtcaccagg
      661 gcgagggcgg ttcgaacgcg tctccttcgg ggacggacct ggtgcgtctc ggaaggctcc
      721 gttcgcggcg ggggcgggag gacccgcaga ggcggtggct cggcgatctt cgtccgcgcg
      781 agcggcgcct gatccacccg ttcggtcggt gccgtggacg agacaggctc ctcgccggag
      841 gcctggatcc ccgacggcat gagcgcctgc ttggtcgcct ccagcagctc ggcgcacgtg
      901 gcgaaccgct gctcagggtc cttggccatg gcccgcgcca ccacgacgtc gaggccggtc
      961 gggagctcgg gccgcaccga cgtgacgggc gggggttcgt tctggaggtg gccgtacatc
     1021 accccagcgt cggtggtctc cgggaacggg accgagcccg tgaggcattc gaacaggacg
     1081 cagcccaggg agtagatgtc ggcgcggcca tcgagctcct cgccgcggaa cacctcgggc
     1141 gccgtgtagt cgagggtccc caccaggtgg ccggtccggg tgagcttcgt gggggtcagg
     1201 agccgcttca ctaggccgaa gtcggtcagg aacgcgcgct cccggccgcc ggccgtccgg
     1261 gagggttcga tcatgacgtt gccgggcttc acgtcccggt gcaggagccc gtgggcgtgg
     1321 gcgaaatcga gagcccggcc cacggcggcc atcacccgga aggttcgggc cgggctcagc
     1381 ttgccctcga ggtcgatgag cgcgcccagg tcggttcccc ggacgtagcg catcgcgctg
     1441 tagaggacgc cgtcggcctc cccggcgtcg tagaccggaa tgatgttcgg atgctccagg
     1501 gagccggcca ggtcggactc ccggataaac cgctcccgga actcggggtc ctcggcgagc
     1561 tccgaggaga gcaccttcag ggcggccttg cgggtcgcca tccggacgtg ctgcgcgagg
     1621 tagaccgtcc ccatcccacc gcgcccgatc agccagtcca cccggtaatt ccccaggacg
     1681 gcgccagccc gaagctcggc catgtcacgc gggttgtact gccgaacgac ctcccggcgc
     1741 aatccccggc gcgcagagtg acaggaatga caacggtgtg acttgggtca cggcgtgggg
     1801 gtaggctcgg cggtgggatg aggttcctgc acacggggga ctggcacgtc gggaaggcca
     1861 tccggggccg gtcgcggatg gacgagttcg aacgggccct ggaccaggtg gtgggcatcg
     1921 ccgtggacga acgggtcgac gccgtgctgg tcgccggcga cctgtacgag catcgcagcg
     1981 cggcgccgga cgcggatcgc atggtgttcg agtcgctgct ccggctgcac tcgaacggca
     2041 tcccggtggt ggccatcccg gggaaccacg actccgccct ccggttcgag gcgttcgcgg
     2101 ggttgctgaa ggcggtggac gtggacgtgg cctccagggt gctgcccccg gagcagggcg
     2161 gggtcatcgc gctcggctcc cgcgacggga cgcagacgct ccgcgtggcg tgcgtgccgt
     2221 tcgtgccgga gcggcggttc ggcagcgccg tggagctgtt ccagtcgacc gaggcgtggt
     2281 tccagtccta cgccgagggg atggggaacc tcatggacgc catggccgcg gggttcgagc
     2341 ccgacgcggt caacgtggtc ctggcgcacc tgttcgcggc gggcgcgaag ctcgggggcg
     2401 gggagcgcga ggtcaccgtg gggctggact acgcggtgcc ggcctcccgg ctgccggcga
     2461 acgcgtcgta cctggcgctc gggcacatcc accggcccca gcggatcccg gggagccggg
     2521 cccccgcccg gtacgcggga tcgctgatcc agctcgactt cggcgagacc gaacaggaca
     2581 agtcggtggc catcgtggag gcggatcccg gccgcccggc gaaggtccgt gaggtctcgg
     2641 tgaccgcggg ccggaggctg gtcgacgtcg agggcaccct ggaccaggtc cacatccggg
     2701 cggccgacct cggcgacgcg tttctccgcg tgttcgtcga caccgacggc ccggtcccgg
     2761 gcatcgccga ccaggtgaga gacctcctgc cgaacgccgt ggacgtccac ctccggtacg
     2821 aacgggacga aggggccgcg gcgggcccgc cgctttccag cctgaagccg cgggagcagt
     2881 tcgaggccta cgtccgggcg gagcacggcg ccgatcccga cccggcgctc atggccgcgt
     2941 tcgacgaggt ggccgaggcg gaggagggct gaagagagtg cgcccggtga agctcgagat
     3001 caaggggttc acgtcgttcc gcgacgagca ggtggtggac ttcgacgggc tcgacctgtt
     3061 cgcgatcgtc gggcccaccg gctcagggaa gtcgagcgtg ctcgacgcca tgacctacgc
     3121 gctgttcggc caggtggacc gggtggatgg cagcgagacc cccatgcgcg agatggtcag
     3181 ccagggccag ccccggatgg cggtggcgct ggagttcgag gtcggggccg accggtacag
     3241 ggtcacgcgt tcgatgccgc gctcgggcgc cacgaggatc ctcctccagc ggtgggaggg
     3301 cggggagtgg cggcaggcgg gcgagggggc cgaccgggtc cgtgacgcga accgcaagct
     3361 ccaggacctc atcggcctgg acttcgacgg gttcacccgc tcggtgctcc tgccgcaggg
     3421 gaaattctcc gcgttcatgt cgggcgacgc gaaggagcgc cgcgacatcc tcaccgacct
     3481 cctcgggctg tcgctgttcg accggatggg gaagcgggcc aggcaggtcg cgaaggagtc
     3541 gaaggagaag gccgacaccg tcgaggagct gatcgaatcg cagttcgccg gggccacgcc
     3601 cgaggccctg gacggggccc ggcgggcggc gcagtcggtc gcggagcgcc gggacgccct
     3661 gaccgaggcc ggcgaacggg tgcgggggat cgtcgaccgg tggcgggcgg tcgccaagga
     3721 cgtcgcggat ctccgttcgt gcgcctcgga ggcggccggg ctggcccgga cggcggcccg
     3781 gcacgcggag acgctggagg ccttggccgc ggccgccgcg aaggcggacc ggggcgtgac
     3841 ggacgcggag accgcggccg ccgccgccgg ggaggcgagg gacgcggccg ggtcggcgcg
     3901 cgaggccgcc gagcgcgagt gggggaccgc ggccgacctg gccagggccc acgcggaggc
     3961 cgtgcggctg gccctggccc gaggcgagct gcgccgccgc cggggcgtcc tggagcaggc
     4021 cgagcgggcc gttccggagt ctgccgcggc cgtcgaggaa gccgaacggg cgctggacgg
     4081 agcgaacgcg gaatccgagc gcctgcgatc agaccacggt gccgcggctg cagccctcga
     4141 ggacgttcgc cacgccgacc aggtgtcggc gatggtgtcg aacctggccg tcggcgaccc
     4201 ctgcccggtg tgcggggagc ccctggcgaa gcttcccgat gctcccggcg cgaaggccat
     4261 gaaggccgcc gaacgggggg aggccgggtc ccgcacggcc gcggaagccg cggcgcgggc
     4321 cgccggggag gccgagcggg cgctggaccg ggcccgggcc gcgatgcagg ccgcggagcg
     4381 ggaacgcgat gcggcggccg gggaggccga acgatgcggc atcgaggtcg ccgggctgga
     4441 ggccgcgatc ggcaggctgc tcccggaggc cgtggacgac cccgccgccg agctggcgtc
     4501 ccgctccgcc cgcctggagg ggctggttcg ggccgaacgg gatgcggagg caaccgcgac
     4561 cgccgccgac cgggcggcgc aggaggcccg cggggcccgc gagcgcatcc ggggcgatgc
     4621 cggggcgtcc cgggccggac tggatggcat ctcggtggcg tccctggcgg agcgggcgtc
     4681 cgcgatcgcc ccggacctgg aggcacccgc actcccggac cttcccgcgt ccgaggacat
     4741 ccccgcattg gccgtctcgg ctcgcctccg ggcggacgcc ctggccaggt acggcggatc
     4801 cgtggagggg gccgcgaccg accgggccgc ttctgagggc gcgttcgtgg acgaagctcg
     4861 cggcgccgtg accgggctgg ggatcgagcc cggcgccccg gacggcctcg acgggctcgc
     4921 cgccgacgtg gatcgcgcgg tccgggacgc ggtggccgag gcgtccgcgc ggcagggaga
     4981 tgccgagcgg ctcgagctcg acctggccag ggtggccgag ctccgcgaac aggtccggac
     5041 ccttcgggcc cgggccgccg tcttccgcca gctggcccag gagctccagg cggaccggct
     5101 catcgcgttc ctccaggccg aggccctccg cctcctggcg gtggacggtg cgcggcgttt
     5161 gtcggcgctc tcgagcggcc gctacgcgct ggagtacgac cacgacgagt tcctggtgct
     5221 cgaccggtgg aacggcgacg agaccaggag cgtccggacg ctctcgggcg gagagacctt
     5281 cctggcgtcc ctggccctgg cgctggccct ggccgaacag gtctcgagcc tggccgtcac
     5341 cgagcacgcg tcgctcgaca gcctgttcct cgacgagggg ttcggcacgc tcgaccccga
     5401 gaccctggac gtggtggtgg aggccatcga gcagctcggc ggcgacggcc gaatggtcgg
     5461 cgtgatcacc cacgtccggg acctggccga ccgcctcccc gcccgcctcg aggtgacgaa
     5521 gtctccgcga ggcagcacgg tcagccgcgc cgtctgacct cggggatcag gccacccggt
     5581 cgttcctccg cagcgcggcc tcggcggcgt agtagccgca catgccgtgg acgcccgcac
     5641 cggggggcgt cgatgccgag cagaggtaga tgccgtcggc aggggtcgag taggggtcga
     5701 gcgacggcgc cggccgggcg aacaactgga agccgccgtg ggcgccgccg gcgatgtcgc
     5761 ctcccacgta gttcgggttg taccgctcga ggtcggccgg ccccatggtg tggcggccgg
     5821 agaccagggc ccggaagccc ggggcgaacc gagcgatctg gtcctcgatc cgctcggtca
     5881 tgtccacggt cgagccgttc ggcacgtggc agtaggccca gaccgtgtgc cggccctccg
     5941 gggcgcgcgt cccgtcgaac cgggtggcct ggacgaggag cacgaacggc cgctccgggt
     6001 gctctccccg gccgacgtcc gactcggacg ccgcgatctc ctcgagcgtt ccgccgaggt
     6061 gcacggtccc cgcccccgcg cattccgagg cccgccacgg cagcggcccg tccagcgcga
     6121 gatcgacctt gaacaccccc ggcccgtggc ggaaccgccc gagccggtcg cggtaggacg
     6181 cgggcagccg gtcgccggcg atcccggcca gcgccgacgg ggtcaggtcg aacaggtact
     6241 gccgggccgg ggggaggtcg cccatcgacc gccccggggt cccggttcgc acctcgccgc
     6301 cgagcgacct cagcaggccg gccagcgcgt cggggaggcc ctgcgatccg ccggcgacca
     6361 cgggccatcc caccgcgtga gccgccgcgc cgagcagcaa cccgaacgcg gcggtgagct
     6421 gttcgtcgag gggcagcacg gaatgcgccg cgaggccggc gaacagcgcg cgggcccgtt
     6481 cgccgtcgaa cgacccctcc gcgaccgccc gggcagaccg gacaccgtgg agtccgaacc
     6541 ggcccaggag caccgggtgg cgcggcggcc ggagcggacc catgacctgt tcggccaggt
     6601 cgtcgaaccg gtcgacgaac ggctcgaaca gccgccgcca ggccctcccg tcggcgccca
     6661 gccccgaggc cgtctccgtc atcgagcggt cgagcacgac cgccgtcccg tcgtccagcg
     6721 gatgcgccag gggcacgtcg ggatggacga actccagccc gtggtccgcg agcggaaggc
     6781 cccggaagaa cggcgacgcc acggcgaacg ggtggaccga cgagcagatg tcgtggacga
     6841 acccggggat ggtgagtggt cccgatcggc tgcccccgcc aacggcgcgc gaccgttcca
     6901 gcaccagcac cgaccggccc gaccgggcca ggtgcaccgc cgcggccagc ccgttcgggc
     6961 cggccccgac caccaccgcg tccagctcgt ccgccgccat ctccctgccg gagggtatcc
     7021 gcaaccgcac gcccgaggcc gtccggacgt cccccgccac tccgctacga tcgcgctcga
     7081 tggaacccga cctcacgccg cggcgggcct ccgattcgga ggtcgagctg gtgcagctca
     7141 tgacgatcct cgatgcgaac aacgtcggga acgtgcacgg cggctcga
//
LOCUS       DASPWO010000114         5900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1040786, whole genome shotgun sequence.
ACCESSION   DASPWO010000114 DASPWO010000000
VERSION     DASPWO010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040786"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="VEO00_02555"
     CDS             <1..1761
                     /locus_tag="VEO00_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HYU56917.1"
                     /translation="LSAITGIDRLVRWDRVPRPLGVLLLAGIRDRLRRDNLVDTDTVA
                     PRRGEVPPPDPRYRTARTWDGTYNDLKHPAMGSSGTRFGRNVPLRYTFPEKEVDLLSP
                     NPRVVSRELLTRETFVPATTLNLLAAAWIQFEVHDWFSHGKGDWDIAWELPLKADDPW
                     PEHPMRIPRARRDPTWEGNGQPPTYLTEDSHWWDGSQIYGNTEAFAKAVRSGEDGKLR
                     IDEHGLPPRELEAGMDLAGVAGNFWVGLGLLHTLFMLEHNAICDMLKKHHPAWTDQQL
                     FDRARLINAALMAKIHTVEWTPAIIAHPTTQLAMRVNWWGLAGEKVKRRFGRISSSEV
                     LSGIPGARTDHHGVPYSLTEEFTAVYRMHPLIPDQYTFRSAADDVVLEERTFPELNAL
                     HVRERLESVGMANALYSFGIAHPGAITLHNYPTFMQHLERPDGTILDLAATDILRVRE
                     RGVPRYNSFRRLFHLKEARSFEDLTPSRDWQRQLRWVYGGDLERVDLMIGMYAEPPPK
                     GFGFSDTAFRVFVLMASRRLESDRFLSQDYRPEVYTPEGLEWVDDTTMVTVLLRHFPQ
                     LGPALHGVENAFTPWRRVRA"
     gene            1766..2878
                     /locus_tag="VEO00_02560"
     CDS             1766..2878
                     /locus_tag="VEO00_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYU56918.1"
                     /translation="MAWVDLAPEGNVVRILSIDGGGIRGIIPARFLVELEEITGQRIA
                     DLFDIVAGTSTGALIALGLVCPGPDGRPALTAEDVQKIYVLDSPDLFPAVKVRRVVQP
                     GGWVRARPNIYQRIGAVVFPRRFGNARYRANGLEAIIRRNLGEARIADAVTDVVVPTY
                     DWKAGRSMIFSSRAARDGEGPNPTMAAVARGTTAAPTYFPPLKTTLEDGREVILIDGG
                     VAANNPVTCAYAEALLWEQRLARDLDVMVVSLGTGRPPEEIPTYEELWSRGWLRLGMG
                     MLNVVFDGTSEISDEMIRTIILKQEPNSRYWRFNTELRGVSLRLDDSSDQNLAGLLRL
                     AQTMIADQREALEDLAGLLVKQVPCLAPRPATGPRT"
     gene            complement(2875..3219)
                     /locus_tag="VEO00_02565"
     CDS             complement(2875..3219)
                     /locus_tag="VEO00_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56919.1"
                     /translation="MVGFLWGRRRRRDPLDNPPFLAIAHRMGLWGGFMLLGLVWAVQL
                     SDLSSGLEIVAASLLVASSVFQDVGAVLNWTLGVKDQFAEHSLGLRFGTANSVFGTAG
                     LAILLIGVVRAL"
     gene            complement(3279..>5900)
                     /gene="aceE"
                     /locus_tag="VEO00_02570"
     CDS             complement(3279..>5900)
                     /gene="aceE"
                     /locus_tag="VEO00_02570"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013475626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HYU56920.1"
                     /translation="LESLDNVIKTEGRTRARYLMLRLLERAREHQVGVPGLRSTDYIN
                     TIPPEQEPYFPGDEQLERRIRRLVRWNAAVMVSRANKHHEGIGGHLSTYASAASLYEV
                     GFNHFFRGKDHAGGGDQVYFQGHGAPGIYARAFLEGRLTEEQLEYFRREAFGLGLSSY
                     PHPRLMPEFWEFPTVSMGLSPLAAIYQARFNRYLHHRGIKDTSGQRVWGFFGDGEMDE
                     PESLAALSLAAREGLDNLIFVVNCNLQRLDGPVRGNGKIIQELESEFRGAGWHVIKVI
                     WGREWDELLARDHDGVLVNKMDTTPDGEFQKYAVESGAYIREHFFGPDPRLQSIVSHL
                     SDDDLVRLRRGGHDYRKLYAAYASAVELTDAPVVILAKTVKGWTLGGTIAGRNVTHQL
                     KKMSEKELESFRDLLELPIKDEEITEGVAPYYRPPEDSVEVQYLLDRRRQLGGFVPER
                     RTRYAQVNLPERDPYAEFLAGSDRSVSTTMAFVALLRNLLRDGVVGSRIVPIIPDEAR
                     TFGMESLFPKFKIYSSKGQLYEPVDSATLLPYVEARDGQILEEGITEAGSMGSFIAAA
                     TSYATHGEAMIPFFIFYSMFGFQRIGDLIWAAADQRARGFLLGATHGRTTLNGEGLQH
                     EDGHSLLLASSNPAVLAYDPAFAFETAVIVREGLRRMFEANEDLIYYLALYNENYPQA
                     AMPEGADERIVKGLYRFADAPELQNGAKRATLIGSGAILQQALAARKLLAEDFEVAAE
                     VWSAPSFQQLREEALDAERWNRLHPGEARRVPYVIEALEAAEGPVIAVSDSMKAVPDQ
                     IGRWVPGLYLPLGTDGFGRSDTREGLRRFFEIDAPHVVVAALSALAEQGEVKPELVAD
                     AIKRYDLDPDRSNPRNG"
BASE COUNT          915 a         2114 c         2022 g          849 t
ORIGIN      
        1 ctgtcggcca tcacggggat cgaccggctg gttcggtggg atcgggtgcc gcggccgctg
       61 ggggtcctgc tgctcgccgg gatccgcgac cggctgcggc gggacaacct ggtcgacacc
      121 gacacggtcg ccccgcggcg cggggaggtc ccgccgcccg atccccgcta ccggacggcc
      181 cggacctggg acgggacgta caacgacctg aagcacccgg cgatgggctc ctccggcacg
      241 cggttcggcc gcaacgttcc cctgaggtac accttcccgg agaaggaggt ggacctcctc
      301 tcccccaacc cgcgcgtcgt cagccgcgag ctcctcaccc gcgagacgtt cgtccccgcc
      361 accacgctga acctgctcgc ggcggcctgg atccagttcg aggtacacga ctggttcagc
      421 cacggcaagg gcgactggga catcgcatgg gagctgccgc tgaaggccga cgacccgtgg
      481 cccgagcacc cgatgcgcat tccgcgcgcc aggcgcgacc cgacctggga ggggaacggt
      541 cagcccccga cctacctgac cgaggacagc cactggtggg acggctcgca gatctacggg
      601 aacacggagg ccttcgcgaa ggccgtgcgc tccggcgagg acggcaagct ccggatcgac
      661 gagcacggcc tgccgccgcg cgagctggag gccgggatgg acctcgccgg ggtggccggc
      721 aacttctggg tcggcctcgg cctcttgcac acgctgttca tgctcgagca caacgcgatc
      781 tgcgacatgc tgaagaagca ccaccccgcc tggaccgacc agcagctctt cgaccgcgcg
      841 cggctgatca acgcggcgct gatggcgaag atccacaccg tcgagtggac cccggcgatc
      901 atcgcccacc cgaccacgca gctggcgatg cgcgtcaact ggtggggact ggccggcgag
      961 aaggtcaagc ggcgcttcgg ccggatcagc tcgagcgagg tgctgagcgg catccccggc
     1021 gcgaggaccg accaccacgg cgtgccctac tcgctgaccg aggagttcac ggccgtctac
     1081 cggatgcacc cgctgatccc cgaccagtac acgttccgct ccgcggcgga cgacgtggtg
     1141 ctcgaggagc gcaccttccc cgagctgaac gcgctgcacg tccgcgagcg cctggagtcg
     1201 gtcgggatgg cgaacgcgct gtactcgttc ggcatcgccc atcccggcgc gatcacgctg
     1261 cacaactacc cgacgttcat gcagcacctc gaacgcccgg acgggacgat cctcgatctc
     1321 gccgcgaccg acatcctgcg cgtccgcgag cgcggcgtcc cgcggtacaa cagcttccgg
     1381 cgcctgttcc acctgaagga ggcgcgctcg ttcgaggacc tcacgccgag ccgcgactgg
     1441 cagcggcagc tgcgctgggt ctacggggga gacctcgagc gcgtggacct gatgatcggc
     1501 atgtacgccg agccgccccc gaagggcttc gggttcagcg acaccgcctt ccgcgtgttc
     1561 gtgctgatgg cgtcgcgccg cctcgagagc gaccgcttcc tgagccagga ctaccgcccg
     1621 gaggtctaca cgcccgaggg gctggagtgg gtcgacgaca ccaccatggt cacggtgctg
     1681 ctgcgccact tcccgcagct cggcccggcc ctgcacggcg tcgagaacgc cttcacgccc
     1741 tggcggcgcg tccgggcctg agccgatggc gtgggtcgac ctggcgcctg aggggaacgt
     1801 cgtccgcatc ctctcgatcg acggcggcgg gatccgcggg atcatcccgg cccgcttcct
     1861 ggtcgagctc gaggagatca ccgggcagcg catcgccgac ctgttcgaca tcgtcgctgg
     1921 cacgtcgacc ggcgcgctga tcgcgctggg actcgtctgc cccggtcccg acggccgccc
     1981 cgcgctcacc gcggaagacg tccagaagat ctacgtcctg gactcacccg acctcttccc
     2041 ggcagtgaag gtgcggcgcg tcgtgcagcc cggcggatgg gtgcgggcca ggccgaacat
     2101 ctaccagcgg atcggggcgg tcgtcttccc acgccgcttc ggcaacgccc gctaccgcgc
     2161 caatggcctg gaggccatca tccggcggaa cctcggcgag gcgcgcatcg ccgacgcggt
     2221 caccgacgtg gtggtcccga cctacgactg gaaggccggc cggtcgatga tcttcagcag
     2281 ccgcgcagcc cgcgacggcg agggtccgaa cccgacgatg gccgcggtgg cgcgtggcac
     2341 gacggcggcc ccaacctact tcccaccgct gaagacgacg ctcgaggacg ggagggaagt
     2401 gatcctgatc gacggcggcg tggcggcgaa caacccggtc acctgcgcgt acgccgaggc
     2461 gttgctgtgg gagcagcgcc tggcgaggga cctggacgtg atggtcgtgt cgctcgggac
     2521 cggacgcccg ccggaggaga tcccgaccta cgaggagctg tggagccgcg gctggctgcg
     2581 cctgggcatg ggcatgctga acgtggtctt cgacgggacc agcgaaatct ccgacgagat
     2641 gatccgcacg atcatactga agcaggagcc gaacagccgg tactggcggt tcaacacgga
     2701 gctgcgcggc gtcagcctcc gtctcgacga ctcgagcgac cagaacctcg ccggcctcct
     2761 caggctcgcg cagacgatga tcgccgacca gcgtgaggct ctcgaggacc tcgccgggct
     2821 gctggtgaag caggtcccct gcctcgcccc ccgcccggcg accgggcccc ggacctagag
     2881 ggcgcgcacc actccgatca ggaggatcgc caggccggcg gtgccgaaca ccgagttcgc
     2941 ggtgccgaat ctcaggccga ggctgtgctc ggcgaactgg tccttcacgc cgagcgtcca
     3001 gttcaggacc gcgccgacgt cctggaacac cgacgacgcg acgagcaggg atgcggcgac
     3061 gatctccaga ccgctcgaca ggtccgacag ctgcacggcc cagaccaggc ccagcagcat
     3121 gaagccgccc cacaggccca tgcggtgagc gatcgccagg aacggcgggt tgtcgagcgg
     3181 gtcgcgacgg cggcgccgcc cccacaggaa tccgaccacg aacgcgaacc ccaggttcag
     3241 cgtccccccg acgaccagga tcttcgacgc agcgtcgatc agccgttcct ggggttcgag
     3301 cggtcggggt cgaggtcgta gcgcttgatg gcgtcggcga ccagctcggg cttgacctcg
     3361 ccctgctcgg ccagcgcgga gagcgcggcc acgacgacat gcggggcgtc gatctcgaag
     3421 aaccggcgca ggccctcgcg ggtgtccgag cggccgaagc cgtcggtgcc gagtggcagg
     3481 tagaggcccg gcacccaccg gccgatctgg tcggggaccg ccttcatcga atcgctcacc
     3541 gcgatcacgg gcccctcggc cgcctcgagc gcctcgatca cgtacgggac ccggcgggcc
     3601 tcgccggggt gcaggcggtt ccagcgctcg gcgtcgaggg cctcctcgcg cagctgctgg
     3661 aacgacggcg cgctccagac ctcggcggcc acctcgaaat cctccgccag cagcttccgc
     3721 gcggcgaggg cctgctgcag gatcgccccc gacccgatca gggtcgcgcg cttcgccccg
     3781 ttctgcagct ccggcgcgtc cgcgaatcgg tacagtccct tgacgatcct ctcatcggct
     3841 ccctccggca tcgcggcctg agggtagttc tcgttgtaga gggccaggta gtagatcagg
     3901 tcctcgttcg cctcgaacat ccgccgcagg ccctcgcgga cgatcaccgc ggtctcgaag
     3961 gcgaacgccg ggtcgtaggc cagcaccgcc gggttcgagg aggccagcag cagggagtgc
     4021 ccgtcttcgt gctgcagccc ctcgccgttc agcgtggtcc tgccgtgcgt cgcgccgagc
     4081 aggaacccgc gggcgcgctg gtcggcggcc gcccagatca ggtccccgat ccgctggaac
     4141 ccgaacatcg agtagaagat gaagaacggg atcatcgcct cgccgtgggt ggcgtaggag
     4201 gtggccgcgg cgatgaacga ccccatcgac ccggcctcgg tgatcccctc ctccaggatc
     4261 tgaccgtcgc gggcctcgac gtagggcagc agcgtcgccg agtccaccgg ctcgtacagc
     4321 tgtcccttcg acgagtagat cttgaacttc gggaacaacg actccatgcc gaacgtgcgg
     4381 gcctcgtcgg ggatgatcgg gacgatccgc gagccgacca cgccgtcgcg cagcaggttc
     4441 cgcagcagcg cgacgaacgc catggtcgtg gacaccgagc ggtccgagcc ggccaggaac
     4501 tcggcgtagg gatcgcgctc cggcaggttg acctgcgcgt agcgcgtcct ccgctcgggg
     4561 acgaacccgc ccagctgccg gcgccggtcg agcaggtact gcacctccac cgagtcctcc
     4621 ggcgggcggt agtagggcgc cacgccctcg gtgatctcct cgtccttgat cggcagctcc
     4681 agcaggtcgc ggaaggactc cagctccttc tcggacatct tcttcagctg gtgcgtgacg
     4741 ttgcgcccgg cgatcgtccc gccgagcgtc caccccttca ccgtcttcgc caggatgacc
     4801 accggcgcgt ccgtcagttc caccgcgctg gcgtacgccg cgtagagctt ccggtagtcg
     4861 tgccccccgc ggcgcaggcg caccaggtcg tcgtcggaca ggtgcgacac gatcgactgc
     4921 agccgggggt cgggcccgaa gaagtgctcg cggatgtagg cgcccgactc gaccgcgtac
     4981 ttctggaact cgccgtcggg ggtggtgtcc atcttgttca ccagcacgcc gtcgtgatcg
     5041 cgcgccagca gctcgtccca ctcgcgcccc cagatgacct tgatcacgtg ccagcctgcg
     5101 ccgcggaact ccgactccag ctcctggatg atcttgccgt tgccgcgcac cggcccgtcc
     5161 aggcgctgca ggttgcagtt gacgacgaag atcaggttgt cgaggccctc gcgcgcggcg
     5221 agcgagagcg cggccagcga ctccggctca tccatctcgc cgtcgccgaa gaagccccac
     5281 acccgctgcc ccgacgtgtc cttgatcccg cggtggtgca ggtagcggtt gaaccgcgcc
     5341 tggtagatcg cggcgagcgg cgagaggccc atcgacacgg tcgggaactc ccagaactcc
     5401 ggcatcagcc gcgggtgcgg gtacgacgac aggccgaggc cgaacgcctc gcgccggaag
     5461 tactcgagct gctcctcggt gagccgcccc tccaggaacg cccgggcgta gatccccggc
     5521 gcgccgtggc cctggaagta gacctggtcg ccgccgccag cgtggtcctt gccgcggaag
     5581 aagtggttga agccgacctc gtagagcgag gccgccgagg cgtaggtgga gaggtggccg
     5641 ccgatcccct cgtggtgctt gttcgcccgc gagaccatca cggcggcgtt ccagcggaca
     5701 agccggcgga tccgccgctc gagctgctcg tcgccgggga agtacggctc ctgctcgggc
     5761 gggatggtgt tgatgtagtc cgtgctgcgc aggccgggca ccccgacctg gtgctcgcgg
     5821 gcgcgctcca gcaggcggag catcaggtag cgggcgcggg ttcggccctc cgtcttgatg
     5881 acgttgtcga gcgattcgag
//
LOCUS       DASPWO010000115         6969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_102099, whole genome shotgun sequence.
ACCESSION   DASPWO010000115 DASPWO010000000
VERSION     DASPWO010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102099"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VEO00_02575"
     CDS             complement(<1..204)
                     /locus_tag="VEO00_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56921.1"
                     /translation="MSGGARALALAFVLVACARGTGNVDSGVQGTVLLGPQCPVVIEG
                     SPCPDLPTAAEVRASDGETGQTIA"
     gene            complement(201..1190)
                     /locus_tag="VEO00_02580"
     CDS             complement(201..1190)
                     /locus_tag="VEO00_02580"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HYU56922.1"
                     /translation="MKDVGLVGLPYAGRTTLFRALTHAGAAGGRANQAVVPVPDERLA
                     VLAEMERSRKVVQAQVLFVDVPGGTGAQGLAALREADALAVVLRAFGADADPARDLAE
                     VTADLIVADLAVLEGVAERAAKKARGRSQEPAASHEAEVLARAALALHGERLLRDAGF
                     DDQEAKILRGAGPLTLKPWVIVANLEEGGSVPDGLPEGTVGVYAAIEAEVAEMDEAEG
                     RALLAEFGVTEPGLPAVIAASYRALDLVTFLTTGEDETRAWQVRRGAHAPEAAGVIHT
                     DLERGFIRAEVVSYDDLVAAGSWENAKHQGHLRVEGRDYLVREGDVLHVRFAV"
     gene            1218..1589
                     /locus_tag="VEO00_02585"
     CDS             1218..1589
                     /locus_tag="VEO00_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HYU56923.1"
                     /translation="MAPRTASRTSKLRAAAKPEPRQPVRLTSSRVLVKHPEAEGERRS
                     KAGLLIPATAASVSRKCIWSEVVAVGPEVRGIEAGDRVLFLPDAGLDVEVLDEDYLLL
                     RERDIHAVASERIEIQTGLYL"
     gene            1729..2046
                     /locus_tag="VEO00_02590"
     CDS             1729..2046
                     /locus_tag="VEO00_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYU56924.1"
                     /translation="MLDLGRYTEPALAILVALADGPRHGYGIMEEAEARWGVRLGPGT
                     LYAALARLERDGLISALPPQERRRPYRLTRKGRNELQAQLDGLRRMAASGLRSLRAAR
                     GTA"
     gene            2043..2582
                     /locus_tag="VEO00_02595"
     CDS             2043..2582
                     /locus_tag="VEO00_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56925.1"
                     /translation="MKPAAARRWVRLYPRRWRERYGDELAAILEDLHPTRRPALDLLA
                     GALDARLHPSLPNGGDPVNDIPPHARILSRLALAVLILPTAFLASVSANGFGNGAVHP
                     AVGRFFALPGVEQATVLGPFVALALLAFATARLSVRIEGGALVSTMRLRATALQAVLL
                     GAAALMAVAFVGYWITERF"
     gene            complement(2566..3447)
                     /locus_tag="VEO00_02600"
     CDS             complement(2566..3447)
                     /locus_tag="VEO00_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYU56926.1"
                     /translation="MGVRSDLRRVARGWRWGHPSFGKVDRQAEDFDTSWARRYPAKVA
                     RDAILDLALGPIIAAYCHPRVVGAGNLAGLRHPVVFAANHTSHLDTPVTLRALPRAWR
                     DRTAVVAAADYFYKRRSMGALVSLTFNTVPIERRRLSETSARRIERLIHDHWSVLVFP
                     EGTRSEDGWVGIVRGGAGFLSVANGIPLVPIYVSGTFEAMPRGRSWPRHHPVTVYIGT
                     PLHPQPGEDHRGMTRRFRAALDRLADESRTDWWQAALRAGRGESPPSTGPHAAQWRRS
                     WERTRPRAGFRGIRTSR"
     gene            complement(3463..5040)
                     /locus_tag="VEO00_02605"
     CDS             complement(3463..5040)
                     /locus_tag="VEO00_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HYU56927.1"
                     /translation="MTRPGAVVEVDRRSPPVLVHHGEGFRLERLPRGSRVVYAPDPVP
                     PLHNPDAAIRHALLHPEGRDPLPALLKPGMKLTIAFDDISLPLPPMKPPDVRQRVLEQ
                     VLEMAAAAGVDDVELIAATSLHRRMTPAELKRAVGERVFRAFWPHALRNHDAEDPDDR
                     AFVGTTDEGEEVEISKRAAESDLLVYVNINLVAMDGGHKSVPVGLGTYRSIRHHHNVR
                     TMQESRSFMDPGHSGLHASAIRMGRLLAGHLPVFTVETTLNNKTFPDEFGFLMKRERE
                     WSVTDQLLFVGAKNGLERMPAQARRTLFQRIVAPHALTGVHAGETEAVHERTLANVHR
                     QQLVRVDGQADVLVAGLPYICPYNVNSIMNPILVACLGLGYFFNLYLGKPLVRPGGVM
                     ILFHPTPYEFHPGHHPSYIDFFEEVLAETTDPLELEAKFEEDYANDPWYRHLYRTGNA
                     YHGVHPFYMWYWCAHALDHLGDVIVVGGDPRAVRRMGFHPATSLADALEIAEQTVGRD
                     PSLTYIRTPPLSIAAVS"
     gene            complement(5037..6248)
                     /locus_tag="VEO00_02610"
     CDS             complement(5037..6248)
                     /locus_tag="VEO00_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYU56928.1"
                     /translation="MSVAVAGPERAEPVRALRFKRSVPRFLAARAVGARVGAVAQALA
                     PLSFDELDPLPLPGAGWVRVRPLLAGICGSDVATVHARTSFYFGPIVSTPFVLGHEIV
                     GVTEGRRVVIRPSLGCAVRGIEPPCRRCQAGDASCERVAFGHLAPGLQTGFCADTGGG
                     WSTGLLAHESQLHDVPADLSDEAAVLIEPLACAVRAVRTAAAAPGDLCVILGAGAVGL
                     LVFAALRHLSGEPVRTVVVAKHARQAAEARRLGAETVVPPRTAARHVRRLTRSDLLEP
                     EYDAPYLSGGADVAFECTGAAATLADAMRLTRPRGTVVLAGMPAPDRLDLTPAWHREL
                     TIRGAYAAGPADVEAAFEIARTADLAPLVGPLFPLARWREAIDHAANAGRLGAIKVAF
                     DVRDESKRESG"
     gene            complement(6329..>6969)
                     /locus_tag="VEO00_02615"
     CDS             complement(6329..>6969)
                     /locus_tag="VEO00_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HYU56929.1"
                     /translation="YLWMRLRSLPRDRWAGAFARLARVAPRLFRTEKADRGAFLRRFY
                     RLFEGLPVDAAERLGRECFNHVTALRIAPEAVRRIREHRRAGHHVVLITGALDFIVRS
                     MEPLVDDLVAARMLARDGRYTGQLADTPPSGEARAAWLRLYAERIGADLSRSYAYGDS
                     LADLPFLQAVGRPVAVNPDTRLAWFASRRGWPVEDWPAMAGPPRILLPKVPA"
BASE COUNT          946 a         2622 c         2498 g          903 t
ORIGIN      
        1 cgctatcgtc tgcccggttt cgccatcgga ggcgcgaacc tccgcggccg tcggcaggtc
       61 cgggcacggg ctcccctcga tcaccacggg gcactgcggc ccgagcagga cggtgccctg
      121 cacgccggag tcgacgttcc ccgtgccacg cgcgcacgcg accaggacga acgccagcgc
      181 gagcgctcga gccccgccgc tcatacggcg aaccggacgt ggagcacgtc gccctcccgg
      241 acgagatagt cgcgtccctc tacccggaga tggccctggt gcttcgcgtt ctcccaggac
      301 ccggctgcca ccaggtcgtc gtaggacacg acctcggcgc ggatgaaccc gcgctcgagg
      361 tccgtgtgga tcaccccggc tgcctccggg gcgtgggcgc cgcgacgaac ctgccaggcg
      421 cgcgtctcgt cctcgccggt ggtcaggaac gtcaccaggt cgagcgcgcg gtaggaagcc
      481 gcgatcacgg cgggcagccc cggctcggtg acgccgaact cggcgagcag cgcgcggccc
      541 tccgcctcgt ccatctccgc cacctcggcc tcgatggccg cgtagacgcc gacggtcccc
      601 tccggcaggc cgtccgggac cgatcctccc tcctcgaggt tcgccacgat cacccacggc
      661 ttcaacgtca acggtccggc tccgcgcagg atcttcgcct cctggtcgtc gaatcccgcg
      721 tcgcgcagga gccgctcccc gtgcagggcc agggcggccc gggccagcac ctccgcctcg
      781 tggctcgccg ccggctcctg gctccgccct cgggccttct tcgcggcgcg ctccgccacg
      841 ccctcgagga ccgcgaggtc ggcgacgatc aagtcggcgg tcacctcggc caggtcgcgg
      901 gcgggatcgg cgtcggcgcc gaaggcgcga agcacgacgg cgagcgcgtc ggcctcgcgc
      961 agcgcggcga gtccctgcgc gcccgtcccg cccgggacgt ccacgaagag aacctgcgcc
     1021 tggacgacct tccgcgagcg ctccatctcc gcgaggacgg ccagtcgctc gtcgggcacc
     1081 ggcaccaccg cctggttggc gcggccgccg gcggcccccg cgtgggtgag cgctctgaac
     1141 agcgtggtcc tcccggcgta ggggaggccg acgagcccga catccttcac ggacggcagc
     1201 ctacaatcgg gctgcccatg gcccctcgga ccgcgtccag aaccagcaag ctccgcgctg
     1261 ccgcgaagcc ggagcccaga cagccggtcc gcctgacctc aagccgcgtc ctggtcaagc
     1321 atccggaggc cgagggtgag cgccgctcca aggccgggtt gctgatcccg gcgaccgcgg
     1381 cctccgtcag ccgcaagtgc atctggtcgg aggtcgtcgc ggtcggtccc gaggtccgcg
     1441 gcatcgaggc cggcgaccgg gtgctcttcc tccccgatgc cgggctggat gtcgaggtcc
     1501 tcgacgagga ctacctcctg ttgcgcgagc gcgacatcca cgccgtcgcc agcgagcgca
     1561 tcgagatcca gaccggcctg tacctgtagt cggctcggcc tcgctccggt accggccgaa
     1621 cgcgacctca ggtcgctccc ccgcggacct gcggcacggc gcgttcgtgc atgctatcgt
     1681 cggacggtat atcgtgcggc gataactcta cacgtgaacg gaggagtggt gctcgacctc
     1741 gggaggtaca cggagcctgc gcttgcgatc ctcgtcgcgc tggccgacgg gccccggcac
     1801 ggctacggca tcatggagga agcggaggcg cgctggggcg tgcggctcgg accgggaacg
     1861 ctctacgcgg cgctcgcccg actggagcga gacggcctga tctcggccct cccgccccag
     1921 gagcgccgcc gtccctaccg gctcacccgg aagggacgga acgagctgca agctcagctc
     1981 gacggcctgc gccggatggc ggcctccggc ctgcggtcgc tgcgggccgc acgggggacg
     2041 gcgtgaagcc ggccgccgcc cgccggtggg tgcgcctcta tccccgccgg tggcgcgagc
     2101 gctacggcga cgagctggcc gcgatcctcg aggacctgca tcccactcgt cgcccagcgc
     2161 tcgacctgct ggccggcgcg ctcgacgccc ggctgcatcc ctcgctcccg aacggaggtg
     2221 accccgtgaa cgacatccca ccgcacgccc ggatcctgtc ccggctggcc ctcgccgtgc
     2281 tgatcctgcc gacggcgttc ctggcatcgg tgagcgcgaa cggcttcggc aacggcgcgg
     2341 tccacccggc cgtcggccga ttcttcgcgc ttcccggcgt cgagcaggcc accgtcctcg
     2401 gcccgttcgt cgcgctggcc ctgctcgcgt tcgccacggc gcggctctcc gtgcgcatcg
     2461 agggcggcgc gctggtcagc accatgcgcc tgcgcgccac cgccctccag gccgtcctcc
     2521 tcggagccgc cgccctgatg gccgtcgcgt tcgtcggcta ctggatcacc gagaggttct
     2581 gatcccccgg aatccggcgc gcgggcgggt gcgctcccag ctgcgccgcc attgcgcggc
     2641 gtgggggccg gtcgacggcg ggctctcgcc gcggccggcg cgcagggcgg cctgccacca
     2701 gtcggtgcgc gactcgtcgg ccaggcggtc gagggcggcg cggaagcggc gggtcatgcc
     2761 ccggtggtcc tcgccgggct gcggatggag cggcgtgccg atgtacaccg tgaccgggtg
     2821 gtggcggggc caggagcggc cgcgcggcat ggcctcgaac gtcccgctca cgtagatcgg
     2881 gacgagcggg atgccgttcg ccaccgacag gaagccggcg ccgccccgga cgatccccac
     2941 ccacccgtcc tccgagcgcg tcccctccgg gaacaccagc acgctccagt ggtcgtggat
     3001 cagccgctcg atccgccgcg cgctcgtctc cgacaggcgg cggcgctcga tcggcaccgt
     3061 gttgaacgtc aggctgacga gcgctcccat cgagcgccgc ttgtagaagt aatcggccgc
     3121 agcgaccacc gcggtccggt cgcgccaggc ccgcgggagc gcccgcagcg tcaccggcgt
     3181 gtccaggtgg ctggtgtggt tggcagcgaa caccaccggg tggcgcaggc cggcaaggtt
     3241 cccggccccc acgaccctcg ggtggcagta ggcggcgatg atcggcccga gggcgaggtc
     3301 gaggatcgcg tcgcgcgcca ccttcgccgg atagcgccgc gcccacgacg tgtcgaaatc
     3361 ctcggcctgc cggtcgacct tcccaaagga cggatggccc caccgccacc ctcgcgcgac
     3421 ccggcgcagg tccgaccgca cgcccaccgc gctcgtggcc ctctacgaga ccgccgcgat
     3481 cgagagcggc ggcgtgcgaa tgtaggtcag cgagggatcg cggccgacgg tctgctccgc
     3541 gatctcgagc gcgtcggcca gcgatgtcgc ggggtggaag cccatccggc gcaccgcgcg
     3601 cgggtcgccg ccgacgacga tgacgtcccc gaggtggtcg agcgcgtgcg cgcaccagta
     3661 ccacatgtag aacgggtgaa cgccgtggta cgcgttgccg gtgcggtaca ggtgcctgta
     3721 ccacgggtcg ttcgcgtagt cctcctcgaa cttcgcctcc agctccagcg ggtccgtcgt
     3781 ctcggccagg acctcctcga agaagtcgat gtacgacgga tggtggccgg gatggaactc
     3841 gtacggcgtg ggatggaaca ggatcatcac gccgccgggc cgcacgagcg gcttcccgag
     3901 gtacaggttg aagaagtacc cgagcccgag gcaggccacc aggatcgggt tcatgatcga
     3961 gttgacgttg tacgggcaga tgtacggcaa tccggcgacc agcacgtcgg cctggccgtc
     4021 gacgcgcacc agctgctgac ggtgcacgtt cgccagcgtc cgctcgtgga cggcctcggt
     4081 ctcccccgcg tgcaccccgg tcagtgcgtg cggcgcgacg atcctctgga acagcgtgcg
     4141 ccgggcctgg gccggcatgc gctccaggcc gttcttggcg ccgacgaaca gcagctggtc
     4201 cgtcaccgac cactcgcgct cgcgcttcat caggaagccg aactcgtcgg ggaacgtctt
     4261 gttgttcagc gtggtctcga ccgtgaagac gggcaggtgc ccggccagca gccggcccat
     4321 ccggatcgcc gaggcgtgca ggccggagtg gccggggtcc atgaacgagc gcgactcctg
     4381 catcgtgcgc acgttgtggt ggtgccggat cgagcggtac gtgccgagcc cgacgggcac
     4441 cgacttgtgg cccccgtcca tcgccaccag gttgatgttc acgtagacca gcaggtcgct
     4501 ctcggcggcg cgcttggaga tctcgacctc ctcgccctcg tcggtcgtgc cgacgaacgc
     4561 gcgatcgtcc gggtcctccg cgtcgtggtt gcgcagcgcg tgcggccaga acgcccggaa
     4621 cacgcgctcg ccgacggcgc gcttcagctc ggccggggtc atccggcggt gcaacgaggt
     4681 ggcggcgatc agctcgacgt cgtcgacgcc ggccgcggcg gccatctcca acacctgctc
     4741 cagcacgcgc tggcggacgt ccgggggctt catcggtggc agcgggagcg agatgtcgtc
     4801 gaacgcgatc gtcagcttca tccccggctt caggagcgcc gggagcgggt cgcggccctc
     4861 gggatgcagc agcgcgtggc ggatggcggc gtcggggttg tgcagcgggg ggaccgggtc
     4921 gggcgcgtag acgacgcggc tgccgcgtgg cagccgctcc agacggaatc cctcgccatg
     4981 gtgcaccagc acgggcgggg atctccggtc gacctcgacc accgctccgg gtctcgtcat
     5041 cccgactccc gcttcgactc gtcgcgaacg tcgaaggcta ccttgatcgc tccgaggcga
     5101 ccggcgttcg ccgcgtggtc gatcgcctcg cgccagcggg ccagcgggaa cagggggccg
     5161 accagcggcg cgaggtcggc cgtccgcgcg atctcgaagg cggcctcgac atcggccggc
     5221 ccggcggcgt aggcgccgcg gatcgtcagc tcgcggtgcc atgccggcgt gaggtccagg
     5281 cggtccggcg ccggcatccc ggccagcacc accgtccccc gcgggcgggt caggcgcatc
     5341 gcgtcggcca gcgtcgcggc ggcgccggtg cattcgaacg cgacgtccgc acccccggag
     5401 aggtacgggg cgtcgtactc gggctccagc aggtccgacc gcgtcagccg gcggacgtgg
     5461 cgggcggcgg tgcgcggcgg aaccaccgtt tccgctccca ggcggcgcgc ctcggccgcc
     5521 tgccgcgcat gcttcgccac gaccaccgtt cgcaccggct cgccggagag atgccgaagg
     5581 gccgcgaaca ccaggagccc gaccgccccg gcgccgagga tcacgcacag gtcgccgggg
     5641 gcggccgcgg cggtgcgcac cgcgcggacc gcgcaggcca gcggctcgat caggacggcg
     5701 gcctcgtcgg agagatccgc cggcacgtcg tgcagctggc tctcgtgagc gaggagcccg
     5761 gtcgaccacc cacccccggt gtcggcgcag aagccggtct gcagccccgg cgccaggtgc
     5821 ccaaacgcga cccgctcgca cgaggcgtcg ccggcctggc accggcggca cggcggctcg
     5881 atcccgcgca cggcgcagcc gagtgacggt cggatcacca ctcgccggcc ctcggtcacg
     5941 ccgacgatct cgtggccgag cacgaagggc gtggagacga tcggcccgaa gtagaacgag
     6001 gtccgggcgt ggacggtggc cacgtccgag ccgcagatcc cggccaggag cgggcggacg
     6061 cgcacccagc cggctcccgg cagcgggagc gggtcgagct cgtcgaacga gagcggcgcg
     6121 agcgcctgcg ccaccgcgcc gacacgcgcg ccgaccgcgc gcgccgccag gaacctcggc
     6181 accgaccgct tgaaccgcag cgcccggacc ggctccgcgc gctcgggccc cgcgacggcg
     6241 accgacacat cccccttggg gggttcgctc gccggacgtg cgcgggtcga tccctccccg
     6301 gggcgagcta cccccccaaa ccccccggtc acgcgggcac cttcggcagc aggatccgcg
     6361 ggggtccggc catcgccggc cagtcctcca ccggccagcc gcggcggctg gcgaaccagg
     6421 ccaggcgcgt gtccgggttc accgccaccg gccgccccac ggcctgcagg aacggcaggt
     6481 cggccaggct gtcgccgtac gcgtaggagc gcgacaggtc ggcgccgatc cgctccgcgt
     6541 acagccgcag ccacgcggcg cgggcctccc ccgacggcgg ggtgtcggcc agttggcccg
     6601 tgtagcggcc gtcgcgggcc agcatgcgcg ccgccaccag gtcgtcgacc agaggctcca
     6661 tcgagcggac gatgaagtcg agcgcaccgg tgatcagcac gacgtggtgg ccggcgcgac
     6721 ggtgctcccg gatccgacga acggcctccg gcgcgatgcg cagcgccgtg acgtggttga
     6781 agcactcgcg ccccaagcgc tcggccgcgt ccacggggag accctcgaac agccggtaga
     6841 agcgccgcag gaacgcgcca cggtcggcct tctcggtgcg gaacagccgc ggcgcgacgc
     6901 gagccagccg ggcgaaggca ccggcccagc ggtcccgcgg tagcgagcgc agccgcatcc
     6961 acaggtagg
//
LOCUS       DASPWO010000116         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_360752, whole genome shotgun sequence.
ACCESSION   DASPWO010000116 DASPWO010000000
VERSION     DASPWO010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360752"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2126)
                     /locus_tag="VEO00_02620"
     CDS             complement(<1..2126)
                     /locus_tag="VEO00_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYU56930.1"
                     /translation="MLQSTSRTARLDASSGSCRAYLARLARIQNEAAARLERAMPEAT
                     VSRRFRIVLDGLTVRLPAAQLPRLVRLPGVAKVYPSLSYTPNTNESPSIIGADLLHQQ
                     TGAMGDGMKIAVVDTGIDQLNPFLSASGLAMPTGFPKGKREWTTAKVIAARSFPGPRS
                     GRQGRIPVNVSEPHGTHVSGIAAGKAGTFAPAGLGHPAVSGLSGVAPRAWLGNYRVFT
                     VPTPAGYSADSPEIIAAFEAAVRDGMNVINFSGGGPAVDPANDALIEAVSNVVAAGVV
                     PVISAGNDRDEFGLGTAGSPGTAPDAISVAAVSNKHVFAPVLTLDSPPLSPPLPDIPF
                     QPGAGYQIPPQWGTIAKQLVDVGTILGTNRSPVDRRVCGPAQNPEAAVNPLPTGSLSG
                     AIALVSRGNCSFVSKAQRVLEAGAIGMVLVDNRPGEATQIPVPVALPAGMVSDLDGQR
                     LQTALAASSGRGNVRIQTGVQQIDTGRSGIVTYFSSAGPTAFGHLLKPDLAAPGSQIL
                     SSVTRAFNSSRFDVFDGTSMAAPHVSGAAALLLQLHPGWTPEEVKSALMSTAGPAWGN
                     TARTEEAPVTLEGAGLVNVMAADDPKLFLDPPSLSFPDLDIGRGAARATRLVTLSDAG
                     GGAGTWQVELHTQSATGGTDVELPSSVVVPAGGGGTTTLSVAATASSSATPGDDYGFV
                     VLRRGADTLRVPYLFLVTRPGLEAVPV"
BASE COUNT          326 a          714 c          864 g          324 t
ORIGIN      
        1 accggcacgg cctccagtcc cgggcgcgtc acgaggaaga gataggggac gcgcagcgtg
       61 tccgcgccgc gccgcagcac gacgaagccg tagtcgtcgc cgggggttgc ggagctcgac
      121 gccgtcgcgg cgacgctcaa cgttgtggtg ccgcccccgc ccgcggggac gacgaccgag
      181 gagggaagct ctacgtcagt gccgcccgtt gccgactgtg tatgcagctc gacctgccac
      241 gtgccggctc caccgccggc atcgctcagc gtgaccagtc tcgtcgcccg agcggccccg
      301 cggccgatgt cgaggtcggg gaacgacagc gaggggggat cgaggaagag cttcgggtcg
      361 tcggcggcca tcacgttcac gaggcccgcg ccctcgaggg tgacgggagc ttcctcggtc
      421 cgcgcggtgt tgccccaggc cggccccgcg gtcgacatca gggccgattt gacctcttcg
      481 ggagtccagc ccgggtggag ctggaggagt agcgcggccg cgccggagac gtggggggcg
      541 gccatgctcg tcccgtcgaa gacatcgaag cgcgaggagt tgaacgctcg cgtgaccgag
      601 gagaggatct ggcttcccgg tgcggcgagg tcgggcttga gcaggtgccc gaacgccgtt
      661 gggcccgcag aggagaagta cgtgacgatt ccgcttcggc cggtgtcgat ctgctgcacg
      721 ccggtctgga tccggacgtt cccgcggccg gatgaggcgg cgagcgccgt ctgcaaccgc
      781 tgcccgtcga ggtccgagac catcccggcg ggaagggcga cggggacggg gatctgggtc
      841 gcctccccag ggcggttgtc gacgagcacc atgccgatcg cgccggcctc gagcacgcgc
      901 tgggccttgg atacgaagct gcagttgccg cgggagacga gcgcgatcgc tcccgacagc
      961 gaaccggtgg gaagcgggtt gaccgcggcc tccgggttct gcgcgggccc gcatacgcgc
     1021 cggtcgaccg gactgcggtt ggtgcccagg atcgttccca catcgacgag ctgcttggcg
     1081 atggttcccc attgcggcgg gatctggtag ccggctccgg gctggaaggg gatgtccgga
     1141 agagggggcg agagcggggg agagtcgagc gtgagcacgg gcgcgaacac gtgcttgttc
     1201 gagacggcgg cgaccgagat cgcgtcgggc gcggttccgg gcgagcccgc ggtcccgagg
     1261 ccgaactcgt cccggtcgtt tccggccgag atcaccggaa cgacacccgc ggcgacgacg
     1321 ttcgagaccg cttcgatcag ggcgtcgttt gccgggtcga ccgcggggcc tccgcccgag
     1381 aagttgatca cgttcatccc gtcccggacg gcggcctcga aggccgcgat gatctccggc
     1441 gagtctgccg agtagcccgc cggcgtcggg acggtgaaga cgcgatagtt gccgagccag
     1501 gcgcgcggcg ccacgccgct cagccctgaa actgctgggt gtcccaggcc ggccggtgcg
     1561 aaggtgccgg cctttcctgc ggcgattccc gatacgtggg tgccgtgggg ctcactcacg
     1621 ttgaccggga tgcggccctg gcggcccgac cgtggcccgg ggaacgagcg cgcggcgatc
     1681 accttcgcgg tcgtccactc gcgcttgccc ttcgggaagc ccgtgggcat ggcgaggccg
     1741 gaagcgctca ggaatgggtt cagctggtcg atgccggtgt ccacgaccgc gatcttcatg
     1801 ccgtctccca tcgcacccgt ctgctggtgc aggagatcgg cgccgatgat cgacgggctc
     1861 tcgttcgtgt tcggcgtgta ggagaggctc ggatagacct tggcgacgcc agggagacgg
     1921 acgagtcgag gcagctgggc agcgggaagc cggacggtca ggccgtcgag gacgatccgg
     1981 aagcggcgcg agactgttgc ctccggcatc gcgcgctcga gacgggcagc cgcctcgttc
     2041 tggatgcggg caagccgggc caggtaggcg cggcaagagc cgctcgaggc gtcgagtcga
     2101 gcggtcctgc tcgtggactg gagcacaagg cggttatgcg ccgcgagtgg cggctcgggg
     2161 agctggacga tgacgcggac gagcccgcgg gagtgcccgg ccgggatcct gatagccccg
     2221 tggcggag
//
LOCUS       DASPWO010000117         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_868417, whole genome shotgun sequence.
ACCESSION   DASPWO010000117 DASPWO010000000
VERSION     DASPWO010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868417"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VEO00_02625"
     CDS             complement(<1..168)
                     /locus_tag="VEO00_02625"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HYU56931.1"
                     /translation="MLRLLTAGESHGKGLVAVLEGLPAGVPLTLKEVSAELARRRHGY
                     GRGGRMKVEQDR"
     gene            complement(304..873)
                     /locus_tag="VEO00_02630"
     CDS             complement(304..873)
                     /locus_tag="VEO00_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56932.1"
                     /translation="MSRRERTLLVILGVIAVGAVGFFLLTRGGGEPVAVPTGPQPTAS
                     PTVLPSPTPSPSPSLPPGGPIFGGRDPFFPLVVAPTAGGGTTSPTTSPTTSPTTSPTG
                     SPTPTVSPAPSPTEGGTVIGDHRVIVLDVFVRGGEEFVQVSVDGQVFTVQEGDTFAGN
                     FRVVAIQDPCATFLFGDEQFEECEQKVGK"
     gene            complement(875..1549)
                     /gene="pilO"
                     /locus_tag="VEO00_02635"
     CDS             complement(875..1549)
                     /gene="pilO"
                     /locus_tag="VEO00_02635"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HYU56933.1"
                     /translation="MSRRTPIFAAIGALALIALVVFLVVLPKSRQVSDTRDQLEEARQ
                     QQAFLEAELARLQGLKAQLPEVRRQLQEFEAKVPATADLPGLIRLLQSASDASGLDFM
                     SVAPGSPAQIAGQDASVIPAEVVIVGGFFQVDEFLFRMETLPRSARVNAIQLGPGPDG
                     LPQLQAALTIQVFSTDPTVGPGAPDPDVPGATATPAPGTSTTPTATPSPTASPTASPT
                     ASPTGG"
     gene            complement(1546..2169)
                     /locus_tag="VEO00_02640"
     CDS             complement(1546..2169)
                     /locus_tag="VEO00_02640"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HYU56934.1"
                     /translation="MTRINLLPPERRERQRARRQTAVVAAVALLVILGLAFFYVLKQL
                     DLSRVQDDLRAQEAVNAKLERDVNDLRQFDQLVAQIEAKRRLLAVVLQNEVQWSGVLR
                     DVSLVIPADVWLSSLNGQITDPFAAGGVTDLAALNGLIGTISMDGFANRHPTVATWLT
                     RLAQVRGFLNPWIATSTESDIGEIPVVQFQSSVDLGQAVTVQGRQRV"
     gene            complement(2166..3221)
                     /gene="pilM"
                     /locus_tag="VEO00_02645"
     CDS             complement(2166..3221)
                     /gene="pilM"
                     /locus_tag="VEO00_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HYU56935.1"
                     /translation="MSRSRIGLDVGSRAVRAAELSLSSDPPELVRAAQVALPPGAVES
                     GEISDPAAVAQGIRELWSRGGFRGKQVVMGVGNQRVIVREVTVPALPEKELRASLPFQ
                     VQEFIPLPVDEAVLDFDVIEEYEEEGRRLLRLLLVAAHREMVNRHVQAALEAKLDPVA
                     IDLVPFALVRAIGQSDGMGLETAGVTECVVDVGADVTNLCVHEQGTPRFVRILPSAGQ
                     DVTVAVGRALGLEDEAAERAKRGVPVEEGPPPEEVQRIAAGRATAFVDELRSSLDFYL
                     SQTPGARVGRVVVSGGGSRLPGLVDLLAARLNAEVAIGHAFQKVTPRLSMEPTELEAA
                     EPVLAVAIGLALPPEQG"
     gene            complement(3218..3451)
                     /locus_tag="VEO00_02650"
     CDS             complement(3218..3451)
                     /locus_tag="VEO00_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYU56936.1"
                     /translation="MERLMPPRRRSFGVSDRLLTVGEVAETMRVSNMTVYRLIKSGQL
                     AAIRVGKNYRIRESDVDRYLSERSVRVDGQEGS"
BASE COUNT          563 a         1381 c         1327 g          501 t
ORIGIN      
        1 ccggtcctgt tcgaccttca tccgcccgcc gcggccgtac ccgtgccgcc gccgcgcgag
       61 ctccgcggaa acctccttca gggtcagcgg cacgccggcc ggcaacccct cgagcacggc
      121 aaccagcccc ttcccatgcg actcacccgc ggtcagcagc cgaagcatgg attcagtgta
      181 ggcatcgccc ggtgccgagc ggggaaaggg gagacggccc gatggactcg ggccgtctcc
      241 gtgggccggc ggccgctccc cccggtgtgg ccgccggaca tctcggtgcg cgcgggacgc
      301 tcgttacttg cccaccttct gctcgcattc ctcgaactgc tcgtcgccga acaggaacgt
      361 cgcgcacggg tcctggatcg cgacaacccg gaagttcccg gcgaaggtgt cgccctcctg
      421 caccgtgaag acctgcccgt ccaccgacac ctggacgaac tcctcgccac cccgcacgaa
      481 cacgtcgagc acgatcaccc gatggtcccc gatcaccgtg ccgccctccg tcggcgacgg
      541 ggccggcgag accgtcggcg tggggctccc ggtcggtgac gtcgtcggcg aggtggtcgg
      601 ggacgtggtc ggcgaggtgg tcccgccgcc cgcggtgggc gcgaccacca acgggaagaa
      661 ggggtcgcgg ccgccgaaga tcggcccacc cggcgggagg ctcggcgacg ggctcggcgt
      721 cggcgacggc aggacggtcg ggctcgccgt gggctgggga cccgtcggga cggccaccgg
      781 ctcgccgccg ccgcgggtca gcaggaagaa gcccaccgcg cccacggcga tcacgccgag
      841 gatcaccagc agcgtgcgct cgcgtcggga cacatcagcc tccggtcggc gacgccgtcg
      901 gagaggccgt cggcgaggcc gtgggcgagg gggtcgccgt gggggtggtc gacgtccccg
      961 gcgcgggggt cgcggtcgcg ccggggacgt cgggatcggg ggcgcccgga ccgacggtcg
     1021 ggtccgtgga gaagacctgg atcgtcagcg cggcctgcag ctggggcagc ccgtcggggc
     1081 cggggccgag ctggatggcg ttcacgcgcg cggagcgcgg cagcgtctcc atccggaaca
     1141 ggaactcgtc gacctggaag aagccgccga cgatcacgac ctctgccggg atcaccgacg
     1201 cgtcctggcc ggcgatctgc gccggggacc cgggagcgac cgacatgaag tcgaggcccg
     1261 aggcgtccga ggccgactgc agcaggcgga tcagccccgg gaggtcggcc gtggcgggaa
     1321 ccttcgcctc gaactcctgg agctggcggc gcacctccgg gagctgcgcc ttcaacccct
     1381 ggagccgggc cagctcggcc tccaggaacg cctgctgctg gcgcgcctcc tcgagctggt
     1441 cccgcgtgtc cgacacctgc cgcgacttcg gcagcacgac caggaacacc acgagcgcga
     1501 tcagggccag ggcgccgatc gcggcgaaga tcggggtgcg ccggctcata cgcgctgcct
     1561 cccctgcacg gtgacggcct gtccgaggtc gacggatgac tggaactgca ccaccgggat
     1621 ctcgccgatg tccgactcgg tcgaggtcgc gatccacggg ttcaggaacc cgcggacctg
     1681 ggccaggcgg gtcagccagg tggcaacggt cgggtgccgg ttggcgaagc cgtccatcga
     1741 gatggtcccg atcaggccgt tcagcgcggc gaggtcggtc acgccgcccg ccgcgaacgg
     1801 gtcggtgatc tggccgttca ggctcgaaag ccacacgtcg gcggggatga cgagggagac
     1861 atcgcgcagg acgccggacc actgcacctc gttctgcagc accaccgcga gcagccggcg
     1921 cttggcttcg atctgcgcca ccagctggtc gaactgtcgc aggtcgttca cgtcccgctc
     1981 caacttcgcg ttgaccgcct cctgcgcgcg gaggtcgtcc tgcacgcgcg acaggtcgag
     2041 ctgcttcaac acgtagaaga acgccaggcc gaggatcacc agcagggcga ccgcggcgac
     2101 gaccgcggtc tggcggcgcg cccgctgccg ttcgcgccgc tccgggggga gcaggttgat
     2161 ccgggtcatc cctgctccgg cggcagggcg agcccgatcg caacggcgag cacgggctcg
     2221 gcggcctcga gctcggtcgg ctccatcgac agccgggggg tgaccttctg gaaggcgtgc
     2281 ccgatcgcga cctcggcgtt cagccgcgcg gcgagcaggt ccaccaggcc cggaagcctg
     2341 gacccgccac cgctgacgac cacgcggccg acgcgggctc ccggcgtctg agagaggtag
     2401 aagtcgagcg aagagcgcag ctcgtcgacg aaggcggtcg cacggccggc cgcgatgcgc
     2461 tggacctcct cgggcggcgg cccttcctcg acgggaacgc cgcgcttggc gcgctcggcg
     2521 gcctcgtcct ccagccccag ggcccggccg accgccaccg tgacgtcctg gccggccgag
     2581 ggaaggatgc gcacgaagcg cggcgtgccc tgctcgtgga cgcagaggtt cgtcacgtcc
     2641 gcaccgacgt ccaccacgca ctcggtgacg ccggcggtct ccaggcccat gccgtcgctc
     2701 tgcccgatcg cccgcacgag tgcgaacggg accaggtcga tggcgacggg atcgagcttg
     2761 gcctcgagcg ccgcctggac gtggcggttg accatctcgc ggtgcgcggc gaccagcagg
     2821 aggcgcagca gccgccgccc ctcctcctcg tactcctcga tcacgtcgaa gtcgaggacg
     2881 gcctcgtcca ccggcagggg aataaactcc tgcacctgga acggcaggga cgcgcggagc
     2941 tccttctccg ggagcgccgg gaccgtcacc tcgcggacga tcacccgctg gttgcccaca
     3001 cccatcacca cctgcttgcc gcggaacccg ccccggctcc acagctcgcg gatgccctgc
     3061 gccacagcgg cggggtcgct gatctccccg ctctccacgg ccccgggcgg cagggcgacc
     3121 tgggccgccc ggaccagctc ggggggatcc gaggacagcg agagctccgc ggcgcgcacg
     3181 gcgcgcgagc ccacgtcgag accgatccgg ctgcggctca cgagccttcc tggccgtcca
     3241 cacggaccga gcgctcggac aggtagcggt ccacgtcgga ctcgcggatc cggtagttct
     3301 tgcccacccg gatcgccgcc agctggccgc tcttgatcag gcggtagacc gtcatgttcg
     3361 agacgcgcat cgtctcggcc acctcgccca ccgtcagcag gcggtccgac acgccgaagc
     3421 tgcgccggcg cggcggcatc aggcgctcca ctcgttcacc atcgggttcc ccttccacgt
     3481 cctgcgcgga cacctcacct gccacaagtt caccgttggt accatcttca acatggtcac
     3541 cagcttcaac atgaggaaca tcctgctcgt cgggaatcgc aacgtactcc gctcccgctc
     3601 gttgtcaaac accacggaag cgttcagtgg ttcactcgtg ctcaatcaca tcactgtaat
     3661 tcgctgtgag cgacagtcgc gacggcagtg ccccgccggc gccgtctggc ccggggtcag
     3721 cggccgagca acgagaggta ggcgtcggcg atcggcgggg cgaacagggc gg
//
LOCUS       DASPWO010000118         4879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_868441, whole genome shotgun sequence.
ACCESSION   DASPWO010000118 DASPWO010000000
VERSION     DASPWO010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868441"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VEO00_02655"
     CDS             <1..843
                     /locus_tag="VEO00_02655"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HYU56937.1"
                     /translation="DAVITMLTEKVDAELLRAAGPQLRIVANHAVGFDNVDLEACTAA
                     GVLATNTPDVLTETTADTAWALLMAAGRRIGEGERFLRARKPWIWGPLMMLGQDVHHK
                     TIGIVGFGRIGQALAHRALGFGMRVLYTDAVELPDDVTGPLRAERRELDELLRDADFI
                     SIHVNLTPETRHLFGAEQFRRMKRTAVLVNTSRGPVIDEAALAEALRAGEIFAAGLDV
                     FEREPEVHEGLLDLENVVVIPHLGSATVDTRHAMGMLCVENVFAALDGRRPKTLLNPE
                     ALER"
     gene            complement(818..1936)
                     /locus_tag="VEO00_02660"
     CDS             complement(818..1936)
                     /locus_tag="VEO00_02660"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HYU56938.1"
                     /translation="MHVLVAPDKFRGTLTAAEAARAIGAGWRRARPQDEVEELPLADG
                     GEGTLDALVAALGGERRSLRVRGPLGDPVDADYGLVSGRHTGVVEMARASGLLLVPEG
                     RRDVFRATTYGTGELIRAACRDGAREVVVCIGGSATNDGGAGMAQAVGARLVDGAGAD
                     VRPGAAGLLDLAAIDVRGLDRAVRDARFMVACDVDNPLTGPQGAAAVYGPQKGAAPDD
                     VVVLDRALGHLAAVIHRDLGIDVRDLPGAGAAGGLGAGLVAFLGARLRPGAEVVKDAV
                     GFEERLARADLVITGEGTLDEQSLRGKLAASVLSSAHQAWTPVLILCGRAQIGVQGVR
                     VASLVNRFGERRALGEARRALEDLAFEAASATAPEPPG"
     gene            complement(2043..2852)
                     /locus_tag="VEO00_02665"
     CDS             complement(2043..2852)
                     /locus_tag="VEO00_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYU56939.1"
                     /translation="MNTVIAAIDNSAAAGPVLDAALALAPLFEGEVEALHVTENGFTI
                     ARQAAASARLPLRTMRGPTAESLVEAGAAEDVVALVVGTRGMPGGRYPAGRTALQVIT
                     TLGKPLVMVPPASTGPHHLERVLVPLEGTAESAHTVRSIVEMAARLDLEVVVLHVATD
                     GTIPRFEDQPHHETEAWALEFVARYAPSPTARVRLELRAGVPGEEVVGVARDVAADLV
                     ILGWSQDLSPDRAQVVREVLGHSPAPVLLLPAAALSSADPVAAGRHTMGSR"
     gene            complement(2849..>4879)
                     /locus_tag="VEO00_02670"
     CDS             complement(2849..>4879)
                     /locus_tag="VEO00_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HYU56940.1"
                     /translation="EPEPERLREALLTLADGRIGTSGAPPLEHPAAQPLVLAAGVYTG
                     TGPATELLHAPLWDRLDGDLGRASGLRRALDLRTGTLRQDLDLPEGRLRTFAFSSLDR
                     PGTAVLRAEGRVPAAMPLSAEDPHTDRTGTAGGWSWIERDSDPVGGIAAAAADRRSED
                     GERLERLVAYAADGEREPEAGEALLALRAAEAAGFEELLAAHRARWADRWEEADVEIA
                     GDPGLQRDVRFALFHLMASVADRGEAAVGARGLSGPAYRGHVFWDADVFVLPFLAGTH
                     PEAARAMLEYRIRRLPAALEAARTIGRAGARFPWESARSGLDVTPKSARDRTGRLMAI
                     RTGYLEEHIVADVAWAAGCYLDWTGDKAFAAGPGGTLLVETARYWASRIRMDHGGIAH
                     VYGVLGPDEYHDAVDDNAYTNVMARWNLRRAAALAEDAGLADEREREYWIALAHALSD
                     GYDPDSRLYEQFAGFFGLEPLVIADVAPSRPIAADLLLGHDRVLAAQVLKQADVLMLH
                     HLVPEEVEPGSLLPNLDFYEPRTAHGSSLSPGIHASLFARAGRFEPALEALRLTSRID
                     LDDLTATTAGGLHLAAMGSLWQALAWGFLGLHPEGDALRIDPRLPPAWEGLRVSARFR
                     GTQLSVEARHGSLDVRADGPARVALEGPGEVDVTAPGRRFVPAGEGWKGQEP"
BASE COUNT          627 a         1862 c         1803 g          587 t
ORIGIN      
        1 gacgcggtga tcaccatgct caccgagaag gtcgacgccg agctcctgcg agcggccggc
       61 ccgcagctgc ggatcgtcgc caaccacgcc gtgggcttcg acaacgtcga cctggaggcg
      121 tgcaccgcgg ccggcgtgct cgccacgaac acgcccgacg tgctgaccga gacgaccgcc
      181 gacacggcgt gggccctgct catggccgcc gggcgccgaa tcggggaggg cgagcgcttc
      241 cttcgcgccc ggaagccctg gatctgggga ccgctgatga tgctcgggca ggacgtccac
      301 cacaagacga tcggcatcgt cgggttcggg cggatcggcc aggcgctggc ccaccgcgcg
      361 ctcgggttcg gcatgcgggt gctctacacc gacgcggtcg agctgccgga tgacgtgacc
      421 gggccgctcc gcgccgagcg gcgggagctg gatgagctgc tccgggacgc ggacttcatc
      481 tcgatccacg tgaacctgac gcccgagacc cggcacctgt tcggtgcgga gcagttccgg
      541 cggatgaagc ggaccgccgt cctggtgaac acctcgcgcg gtccggtgat cgacgaggcg
      601 gcgctcgccg aggccctgcg ggcgggcgag atcttcgccg ccgggctgga cgtgttcgag
      661 cgcgagcccg aggtgcacga ggggctcctc gatctggaga acgtcgtggt catcccgcac
      721 ctcgggagcg ccaccgtcga cacgcgtcac gccatgggga tgctgtgcgt ggagaacgtc
      781 ttcgcagcgc tcgacgggcg ccggccgaag actctcctca acccggaggc tctggagcgg
      841 tagcgctcgc cgcctcgaac gccagatcct cgagcgcccg ccgcgcctcg ccgagcgccc
      901 ggcgttcccc gaagcggttc acgagcgacg ccacgcggac gccctgcacg ccgatctggg
      961 cgcgcccgca caggatcagg accggcgtcc acgcctggtg cgcgctggag aggacgctcg
     1021 ccgccagctt gccgcgcagc gactgctcgt cgagcgtgcc ctcgccggtg atcacgaggt
     1081 ccgctcgcgc caggcgctcc tcgaagccga cggcgtcctt caccacctcc gcccccggcc
     1141 gcaggcgcgc gccgaggaag gccaccagcc cggcccccag gccgccggcc gcgccggccc
     1201 ccgggaggtc gcgcacgtcg atcccgaggt cgcgatggat cacggccgcc aggtggccca
     1261 gcgcccggtc gaggacgacg acgtcgtccg gggccgcccc cttctgcggc ccgtagaccg
     1321 ccgcggctcc ctgcggcccg gtcagcgggt tgtccacgtc gcaggccacc atgaaccggg
     1381 cgtcgcggac ggcgcggtcg agcccgcgca cgtcgatggc cgcgaggtcg agcagtcccg
     1441 cggcccccgg tcggacgtcc gcgcccgcgc cgtccaccag ccgggcgccg accgcctgcg
     1501 ccatacccgc tccgccgtcg ttcgtggcgc tcccgccgat gcacacgacg acctccctcg
     1561 cgccgtcgcg gcaggcagcc cggatcagct cgccggtgcc gtacgtcgtc gcgcggaaca
     1621 cgtcccggcg gccctcggga acgagcaaga gtcccgatgc acgggccatc tcgaccacgc
     1681 cggtgtgccg gcccgagacg agcccgtagt ctgcgtccac cgggtcgccg agcggaccgc
     1741 gaacccggag ggagcggcgc tcacccccga gcgcggcgac cagggcgtcg agggtgccct
     1801 cgccgccgtc cgccagcggg agctcctcga cctcgtcctg cggccgggcg cggcgccagc
     1861 cggcgccgat cgcccgggcg gcctcggcgg ccgtcagggt gccgcggaac ttgtcgggag
     1921 cgacgaggac gtgcatgcga gagcggcccg gatctgccgg cctccgtccc tggatgatag
     1981 ggagaacccg aggtcccgct ccgcgacgcc ggcgtcagat cgggagcgac acggcggccc
     2041 ccctatcggc tccccatggt gtgtctccca gccgccaccg ggtccgccga ggagagtgcc
     2101 gcggcgggga gcagcagcac cggcgcaggg ctgtggccga gcacctcgcg gacgacctgg
     2161 gcgcggtcgg gggagaggtc ctgggaccag cccaggatca ccaggtcggc ggcgacgtcg
     2221 cgggcgacgc cgaccacttc ctcaccgggg accccggcgc gaagctccag ccgtacccga
     2281 gcagtggggg agggcgcgta gcgcgccacg aactccaggg cccaggcctc ggtttcgtgg
     2341 tgcggctggt cttcgaaccg cgggatggtc ccgtccgtcg cgacgtgcag gaccaccacc
     2401 tcgaggtcga gccgggcggc catctcgacg atgctccgga ccgtgtgcgc cgactccgcg
     2461 gtcccctcca gcggaaccag cacgcgctcc aggtggtgcg gcccggtcga tgccggcggc
     2521 accatcacca gcggcttccc gagggtcgtg atcacctgca gggccgtgcg ccccgccggg
     2581 tagcggccgc ccggcatccc gcgcgtgccg acgacgagcg ccaccacgtc ctcggcggcg
     2641 ccggcctcga ccaggctctc cgcggtgggg ccgcgcatgg ttcgcagcgg gaggcgcgcg
     2701 gacgcggccg cctgccgcgc gatcgtgaag ccgttctccg tgacgtgcag cgcctcgacc
     2761 tcgccctcga acaagggcgc gagcgcgagg gccgcgtcga ggaccgggcc ggcggcggcg
     2821 ctgttgtcga tcgcggcgat gaccgtgttc atggctcctg tcctttccat ccttctcctg
     2881 ccggcacgaa ccgtctgccc ggtgcggtca cgtcgacctc cccgggcccc tccagcgcca
     2941 cgcgggccgg gccgtccgct cgaacgtcga gcgagccgtg acgggcctcg acggagagct
     3001 gcgtgccgcg gaaccgtgcg gagacgcgaa gcccctccca cgccggtggg agccgcggat
     3061 cgatccgcag cgcgtctccc tccgggtgca ggcccaggaa gccccaggcg agggcctgcc
     3121 acaggctccc catcgcggcc aggtgcagcc cgccggcggt cgtggccgtc aggtcgtcga
     3181 ggtcgatccg gctggtgagc cgcagcgcct cgagcgccgg ctcgaaccgg ccggcgcgcg
     3241 cgaacaggga ggcgtggatc ccgggggaca gcgagctgcc gtgggcggtg cggggctcgt
     3301 agaagtcgag gttcggcagc agggagcccg gctcgacctc ctccggcacg aggtggtgga
     3361 gcatcagcac gtcggcctgc ttcagcacct gcgcggcgag gacgcggtcg tggccgagca
     3421 gcaggtcggc ggcgatcggc cggctcggcg cgacgtcggc gatcaccagg ggctcgagcc
     3481 cgaagaagcc cgcgaactgc tcgtacagcc gcgagtccgg gtcgtacccg tcggagagcg
     3541 cgtgggcgag cgcgatccag tactcccgtt cgcgctcgtc ggcgagccct gcgtcctcgg
     3601 cgagcgcggc cgcccggcgc aggttccagc gggccatcac gttcgtgtag gcgttgtcgt
     3661 cgaccgcgtc gtggtactcg tcgggcccga gcacgccgta gacgtgtgcg atcccgccgt
     3721 ggtccatccg gatccgcgag gcccagtagc gggccgtctc gaccaggagc gtgccgcccg
     3781 gcccggcggc gaaggctttg tcgcccgtcc agtccagata gcaccccgcg gcccacgcca
     3841 cgtcggccac gatgtgctcc tccaggtagc cggtgcggat cgccatgagc cgcccggtgc
     3901 ggtcccgggc gctcttcggg gtgacgtcca accccgagcg cgccgactcc cacgggaagc
     3961 gggcgccggc ccgtccgatc gtgcgggccg cctcgagcgc ggcgggcagc cgccggatcc
     4021 ggtactcgag catcgcccgc gccgcctcgg gatgggtccc cgccaggaac ggcaggacga
     4081 agacgtccgc gtcccagaac acgtggccgc ggtaggccgg cccgctcagg ccgcgcgccc
     4141 cgacggccgc ctcgccccgg tcggccacgg acgccatcag atggaagagc gcgaagcgca
     4201 cgtcgcgctg caggcccggg tccccggcga tctcgacgtc cgcctcctcc caacgatcgg
     4261 cccaccgggc gcgatgcgcc gccaggagct cttcgaaccc ggccgcctcg gccgcccgca
     4321 gcgccagcag cgcctcaccg gcctcgggct cccgttcgcc gtcggcggcg taggccacca
     4381 gccgctcgag gcgctcgccg tcctcggacc gccggtcggc ggcagccgcc gcgatccccc
     4441 cgaccgggtc cgagtcccgt tcgatccagg accagccgcc ggcggtcccg gtgcggtcgg
     4501 tgtgcgggtc ttcggcggag agcggcatgg ccgccgggac cctgccctcg gcccgcagca
     4561 cggcggtgcc gggacggtcg agcgaggaga aggcgaacgt gcgcaggcgg ccctccggga
     4621 ggtcgagatc ctggcgcagg gtcccggtgc gcaggtcgag cgcgcgccgc agcccggacg
     4681 cccgcccgag gtccccgtcc aggcggtccc acaggggcgc gtgcaacagc tcggtcgccg
     4741 ggccggtgcc ggtgtacacg ccggcggcga ggaccagggg ctgggcggcg ggatgctcga
     4801 gcggcggcgc gcccgatgtc ccgatccggc cgtcggccag ggtcagcagc gcctcgcgga
     4861 ggcgctcggg ctccggctc
//
LOCUS       DASPWO010000119         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1205206, whole genome shotgun sequence.
ACCESSION   DASPWO010000119 DASPWO010000000
VERSION     DASPWO010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205206"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VEO00_02675"
     CDS             complement(<1..376)
                     /locus_tag="VEO00_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56941.1"
                     /translation="MAGIRRVGTRSVSSVLTLAANRITGVLAGEDIATGDAGHIDGDG
                     KVYRSIGASAEEAADVRGFTAAKIRSGEEAGFELVRAANDGEAETSRPFSSGVTHADG
                     GAEDEEVVGAHILHAAGLEADEL"
     gene            complement(416..>2462)
                     /gene="ftsH"
                     /locus_tag="VEO00_02680"
     CDS             complement(416..>2462)
                     /gene="ftsH"
                     /locus_tag="VEO00_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016638806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HYU56942.1"
                     /translation="EGSGSPTPRAKRGGRASAQQLQKRWPWWASFPLALLLNYLLISV
                     LFPGQPQRVEVSYTFFKQQVGIDNVAEISSRADTIQGAFRQEMAYPPDAGDKAKPAKD
                     FSTVQPSFADPGLETLLNEHGVVINAKPVDEPRNPLLNLLLSFGPTLLLIGGFLWISR
                     RAGGAAGGGLFGLGKSQAKRYDQTAPGQNKVTFADVAGIDEAKEELVEIVDFLKDPKK
                     YTRLGGAAPKGVLLVGAPGTGKTLLAKAVAGEAGAPFFSMGASEFVEMIVGVGASRVR
                     DLFKQAREAAPAIIFVDELDAIGRSRSGVIYGGSSSEQEQTLNQILTEMDGFSSREGV
                     IVLAATNRPEVLDRALLRPGRFDRRVTIQPPDKVGREAILRVHTRGVPLAQGVDLRDV
                     AASTPGLVGADLRNLVNEAALLAARRGEDAVRQDDFMDALEKIILGPARPLVMSPAER
                     ERVAYHEGGHTILGLLVAEADPVNRVTIVPRGMALGVTYQRPQDDRHNYHEGYLRART
                     VGAMGGRAAEEVVYGTRTTGAENDMQQATDLVRQMVTRWGMSNKLGPVTLGPRESPFL
                     GGEGGESSFGFSKPYSETTATLIDAEVRRILQESYDEALRLLREHRRELDALAEALLE
                     HETLKEDAILAVTGLRREPPPDSAAPAGLPAALNGARSVNGRTSVEPSISGPGRNGA"
BASE COUNT          328 a          893 c          824 g          417 t
ORIGIN      
        1 tcagctcgtc ggcctcaagc ccggccgcat ggaggatgtg cgcgccgacg acctcctcgt
       61 cctcagcccc gccgtcggcg tgggtcacac ccgacgagaa cggacgggac gtctcggcct
      121 cgccgtcatt ggccgccctg accagctcga atccggcctc ttcgccgctc ctgatcttgg
      181 ccgcggtgaa accccgcaca tccgctgcct cctcggcgct ggccccgatc gagcgataca
      241 ccttgccgtc gccgtcgatg tggccggcgt caccagtcgc tatgtcctcg ccggccagta
      301 ccccggtgat ccgatttgcc gccaacgtga gcaccgacga cactgaccgt gtcccgactc
      361 ttctgatccc agccatgtat cgatgttcct ttaccccgag cctcgcggct cggcctcatg
      421 ctccatttcg cccagggccc gaaatcgacg gttcgacgct ggtccgtcca ttgactgacc
      481 gcgcgccgtt caaggcggcg gggaggccag ccggcgcggc gctgtccggt gggggctcac
      541 gccggaggcc ggtcacggcc aggatggcgt cctccttcag cgtctcgtgc tcgagaagcg
      601 cctccgccag cgcgtccagc tcgcggcggt gttcgcgcag caggcgcagc gcttcgtcgt
      661 agctctcctg gaggatccgc cgcacctcgg cgtcgatcag cgtggcggtc gtctcgctgt
      721 acggcttgct gaagccgaac gaggactcgc cgccctcgcc gcctaggaag gggctctcgc
      781 ggggccccag cgtgacgggg ccgagcttgt tgctcatgcc ccagcgggtc accatctggc
      841 ggacgaggtc cgtcgcctgc tgcatgtcgt tctcggcgcc ggtcgtccgg gtgccgtaga
      901 ccacctcctc ggccgcccgg ccgcccatcg cgccgacggt cctcgcgcgg aggtagccct
      961 cgtggtagtt gtggcgatcg tcctgcgggc gctggtacgt gacgccgagc gccatccccc
     1021 gcggtacgat ggtgacgcgg ttgacaggat ccgcctcggc cacgagcagg cccagaatgg
     1081 tgtgcccacc ctcgtggtac gccacccgct cccgctcggc cgggctcatg accagcgggc
     1141 gcgctgggcc gaggatgatc ttctccagcg catccatgaa gtcgtcctgg cggacggcgt
     1201 cctcgccgcg ccgggcggcc aacagcgccg cctcgttgac caggtttctc aggtcggcgc
     1261 ccactagccc cggcgtggac gcggcgacgt ctctcaggtc gacgccctgg gccaggggca
     1321 ccccgcgggt gtggacccgc aggatcgcct cccgccccac cttgtccggc ggctggatgg
     1381 tgacacggcg gtcgaagcgg ccggggcgca gcagcgcccg gtccagcact tcggggcggt
     1441 tggtggccgc cagcacgatc accccctcgc gggagctgaa gccgtccatc tccgtcagga
     1501 tctggttcag cgtctgctcc tgctcgctgc tgctcccgcc gtagatcacc ccgctccggg
     1561 agcggccgat ggcgtccagt tcgtcgacga agatgatggc cggtgcggcc tcgcgggcct
     1621 gcttgaacag gtcgcgcacg cggctggcgc ccacacccac gatcatctcc acgaactcgc
     1681 tagcacccat gctgaagaac ggcgcccctg cctcgccggc gaccgccttg gcgagcagcg
     1741 tcttgcccgt cccgggcgcg cccacgagca ggacgccctt gggcgcggcc ccacccagcc
     1801 gcgtgtactt ctttgggtcc ttgaggaagt cgacgatctc gaccagctcc tctttggcct
     1861 cgtcgatccc ggccacgtcg gcgaacgtga ctttgttctg gccgggagcg gtctggtcgt
     1921 agcgcttggc ctggctcttc cccaggccga agaggccgcc ccccgcggcg ccgccggctc
     1981 gcctggagat ccagaggaag ccgccgatga gcaggagcgt tgggccgaag ctgagcagca
     2041 ggttgagcag cgggttgcgg ggctcgtcga cgggcttggc gttgatgacg acgccgtgct
     2101 cgttgagcaa ggtctcgagc ccgggatcgg cgaacgacgg ctgcaccgtc gagaagtcct
     2161 tggccggctt cgccttatcg ccggcgtccg gaggatacgc catctcctga cggaaggcgc
     2221 cctggatcgt gtccgcgcgg gagctgatct ccgcgacgtt atcgattccg acctgttgct
     2281 tgaagaacgt gtaggagacc tccacccgct gcggctggcc cgggaatagg accgagataa
     2341 gcaggtagtt cagcagcagc gccagcggga agctggccca ccagggccat cgtttctgga
     2401 gctgctgtgc ggacgcgcgc ccgcctcgct tggcgcgtgg cgtgggggac cccgaaccct
     2461 cg
//
LOCUS       DASPWO010000120         3889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_361090, whole genome shotgun sequence.
ACCESSION   DASPWO010000120 DASPWO010000000
VERSION     DASPWO010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361090"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2132
                     /locus_tag="VEO00_02685"
     CDS             <1..2132
                     /locus_tag="VEO00_02685"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HYU56943.1"
                     /translation="NPKADTKLLRRAFEFAERSHRGQSRVSGEPFIEHPLEVAHVLAD
                     LGMDTTTIVAALLHDVVEDTDLSLDVIDEEFGPQVAAIVDGLTKLDRITYRSREAQQA
                     ENVRKMIIAMARDIRVLIIKLADRLHNMRTLAALARDKQEEKATETLEIYGPLAHRLG
                     IHQLKWELEDLAFATLYPKRFEEIQNLVGKRASERAEYLAQVADAVQSKLREAKVKAV
                     VQGRPKHLYSVYEKMVLRGKEFNEIYDLVGIRVLVDTVRDCYAALGAIHSIWRPIAGR
                     FKDYIAMPKFNMYQSLHTTVVGPEGRPLEIQIRTHDMHRTAEYGIAAHWRYKDGKKDG
                     KVEGDGELAWLAQMLEWQRDMADPREFMESLKIDLYANQVFVYTPKGDVVALPKGATP
                     IDFAYSIHTEVGHRCIGARVSGRLVPLDYELQTGDTVEVFTSKAADAGPSRDWLSIVT
                     TPRARTKIKQWFSRERREDALEKGRELLQRTLRRQGLPIKRYASGEMLERVAADLKYP
                     GLDALLVSIGAGHVSPLSVVNRLQRLLSDQPEEEEGVGEILSARPVDIRQPDSGVVVP
                     GATDVWVRLARCCEPVPGDEIRGWVTMGQGVSVHRVDCPNLRALIERDPPRVLDVSWR
                     PGKATHFVVSIQVEALDRTRLLSDVAQVLADQHVNILSASSNTGKDRIATLRFTFELA
                     DIAHLSSILSAVKRVDHVFEAARVTPG"
     gene            complement(2182..2364)
                     /locus_tag="VEO00_02690"
     CDS             complement(2182..2364)
                     /locus_tag="VEO00_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016868992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HYU56944.1"
                     /translation="MLALMGLAAKTSEVEINFTVRGIVALGAGILILIFPKVLNYIVA
                     AYLIFIGVVELFDLRF"
     gene            2419..2856
                     /gene="dtd"
                     /locus_tag="VEO00_02695"
     CDS             2419..2856
                     /gene="dtd"
                     /locus_tag="VEO00_02695"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HYU56945.1"
                     /translation="MKVLLQRVSRAAVRVDDRTMGEIGPGFVALVGCGALDRPDEPAR
                     LAEKVWNLRVFEDAAGKMNRSLSDTGGTVLVVSQFTLYADTSGGRRPSFVRAAPPDEA
                     EPRVEAFAAALEELGATVARGVFGADMEVELVNDGPVTLLLEG"
     gene            complement(3366..3515)
                     /locus_tag="VEO00_02700"
     CDS             complement(3366..3515)
                     /locus_tag="VEO00_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56946.1"
                     /translation="MAAGERRGMLPMDGALAELVRAARITLETARAAADDPDGLARSL
                     GEADG"
     gene            3680..>3889
                     /locus_tag="VEO00_02705"
     CDS             3680..>3889
                     /locus_tag="VEO00_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56947.1"
                     /translation="MHELDGVLDRHDQARPLGVHHADHRRERRRPSGPARPGHEHEPL
                     RGSGEVRDRRREAEILKRPDRGRDDA"
BASE COUNT          578 a         1367 c         1404 g          540 t
ORIGIN      
        1 acaacccgaa ggccgacacc aagctcctgc gccgggcctt cgagttcgcc gagcgctccc
       61 accgcggcca gtcgcgcgtc agcggcgaac ccttcatcga gcaccccctc gaggtggcgc
      121 acgttctggc cgacctgggg atggacacca ccacgatcgt cgcggcgctg ctgcacgacg
      181 tcgtcgagga caccgacctc tcgctcgacg tcatcgacga ggagttcggg ccccaggtgg
      241 cggcgatcgt cgacgggctg accaagctcg atcggatcac gtaccgctcg cgcgaggccc
      301 agcaggcgga gaacgtccgc aagatgatca tcgcgatggc gagggacatc cgcgtgctga
      361 tcatcaagct cgccgaccgc ctgcacaaca tgcgcacgct ggccgcgctg gcgcgggaca
      421 agcaggagga gaaggccacc gagaccctgg agatctacgg gccgctggcg caccggctgg
      481 gtatccacca gctgaagtgg gagctggagg acctggcgtt cgccaccctc tacccgaagc
      541 ggttcgagga gatccagaac ctggtcggca agcgcgcgtc cgagcgcgcc gagtacctgg
      601 cgcaggtggc cgacgcggtg cagtcgaagc tgcgcgaggc gaaggtcaag gccgtcgtcc
      661 aggggcggcc gaagcacctc tacagcgtgt acgagaagat ggtgctgcgc ggcaaggagt
      721 tcaacgagat ctacgacctg gtcggcatcc gcgtgctggt cgacaccgtc cgcgactgct
      781 acgcggcgct cggcgcgatc cactccatct ggcggcccat cgccggccgc ttcaaggact
      841 acatcgcgat gccgaagttc aacatgtacc agtcgctgca caccaccgtg gtcggcccgg
      901 agggacgccc gctcgagatc cagatccgca cgcacgacat gcaccgcacg gccgagtacg
      961 ggatcgccgc gcactggcgc tacaaggacg gcaagaagga cggcaaggtc gagggcgatg
     1021 gcgagctggc gtggctggcg cagatgctcg agtggcagcg cgacatggcc gacccgcgcg
     1081 aattcatgga gtcgctgaag atcgacctgt acgcgaacca ggtgttcgtg tacacgccga
     1141 agggcgacgt ggtcgcgctg ccgaagggcg ccacgccgat cgactttgcc tattccattc
     1201 acaccgaggt cgggcaccgc tgcatcggcg cgcgggtctc ggggcgcctc gtcccgctcg
     1261 actacgagct gcagaccggc gacaccgtgg aggtcttcac gtcgaaggcg gccgacgccg
     1321 ggccgagccg tgactggctg tcgatcgtca ccaccccgcg cgcccggacc aagatcaagc
     1381 agtggttctc gcgggagcgg cgcgaggacg cgctggagaa gggccgcgag ctgttgcagc
     1441 ggacgctgcg ccgccagggc ctgccgatca agcgctacgc gtcgggggag atgctcgagc
     1501 gcgtggcggc cgacctgaag tacccgggcc tcgacgcgct cctggtctcg atcggcgcgg
     1561 gccacgtctc gccgctgtcg gtggtcaacc gcctgcaacg gctgctctcc gaccagcccg
     1621 aggaggagga gggcgtgggg gagattctct ccgcccgccc cgtggacatc cgccagcccg
     1681 actccggcgt ggtcgtgccc ggcgcgaccg acgtctgggt ccggctggcc cgctgctgcg
     1741 agccggtgcc cggcgatgag atcaggggct gggtgacgat ggggcagggg gtctcggtgc
     1801 accgggtcga ctgcccgaac ctccgcgcgc tcatcgagcg cgatccaccc cgggtcctcg
     1861 acgtctcctg gcgtccgggg aaggccacgc acttcgtcgt ctcgatccag gtggaggcac
     1921 tcgatcggac ccgcctgctg tccgacgtgg cgcaggtcct ggccgaccag cacgtcaaca
     1981 tcctgtcagc ctcgtcgaac accgggaagg accgcatcgc cacgctgcgc ttcacgttcg
     2041 agctggccga catcgcgcac ctctcgagca tcctgtccgc ggtgaagcgc gtggaccacg
     2101 tgttcgaggc ggcccgggtc accccgggct gacggcgcga gcggcgtccg gaccgtccgc
     2161 gccgtcggag atgctcggtt ctcagaaacg caggtcgaag agctcgacca cgccgatgaa
     2221 gatcaggtac gcggcgacga tgtagttcag gaccttgggg aagatcagga tcaggatgcc
     2281 ggcgccgagt gcgacgatcc ccctgaccgt gaagttgatc tcgacctcgc tcgtcttcgc
     2341 ggcgagcccc atcaacgcaa gcatgtcagc cctccttcgc agcaccgggc atacccggcg
     2401 aggcaagctc caaacgccgt gaaagtcctc ctgcaacggg tgagtcgggc ggccgttcgc
     2461 gtcgacgacc ggacgatggg ggagatcggg ccggggttcg tcgcgctcgt cgggtgcggc
     2521 gccctggatc ggccagacga gccggcgcgt ctggcggaga aggtctggaa cctgcgcgtg
     2581 ttcgaggacg cggccggcaa gatgaaccga tcgctgtccg acaccggcgg cacggtcctg
     2641 gtcgtttccc agttcacgct ctacgccgac acgagcggcg gccggcgccc ctcgttcgtc
     2701 cgggccgcgc cgccggacga ggccgagccc cgggtggagg ccttcgcagc ggccctcgag
     2761 gagctcggcg ccaccgtcgc ccgtggcgtc ttcggcgccg acatggaagt cgagctggtg
     2821 aacgacggcc cggttacgct gctgctggag ggctgagcga tgtggatggc ggcggcctgg
     2881 cacttcctgc ggctcgcgct gaccccggtc cagcggttct cggaggcggt cgaggtcccg
     2941 ttcgtggtcg ccggggtggc cgcgagctcc ttcgtcggac gcctgatctc ctcggtgccc
     3001 cccgtggtct tccccctggc gatgctcacg ctgcgtcggt gctggccggc gtccgcctgc
     3061 agctgcggat ggagccgcgg ctggcggtcg agtgcggcga gagtgaggag ttccgggcgc
     3121 tcggcccgcg gcgggcgccg ggtacgtggt catcgccggc caggaagggt cggccctgga
     3181 ggccgcctgg cgggtgcccc cgaggaggac gtgctggtca cggtgaccgc ctgggccgaa
     3241 cggtcggccg ccgacacccg gcggtaccgg ctgcgggccc gcgacggttg ggaccggccg
     3301 gccgtgaccg aggagggcgg ccgccccgag cgatcccgtc cgggcccgga gggggagcgc
     3361 ccccctcagc cgtcagcctc gccgagcgac cgcgccagcc cgtccgggtc gtcggcggcg
     3421 gcgcgggccg tctcgagcgt gatcctggcc gcgcggacca gctcggcgag ggcgccgtcc
     3481 atcggcagca tcccccgtcg ctcgccggcg gccatgaccg agtagagctg ggatccccgc
     3541 ctcgaggatc tcccggtcgg ccggctcgcc gatctggacg gacgttcgcg tccgtccccg
     3601 ccagatcggt gagcgccgcc gcgaggctgg cggcgtcccg ccccacctcc cgctgctgga
     3661 ccgtcgcgcg cccgggcgga tgcacgaact cgatggggtc ctcgaccgtc acgaccaggc
     3721 acggccgctc ggcgttcacc acgccgacca tcgccgcgag cgccgtcgac cgtcgggacc
     3781 cgctcggccc ggccacgagc acgagcccct gcggggctcg ggcgaggtcc gcgaccggcg
     3841 gcgggaggcc gagatcctga agcggcccga ccgcggccgc gacgacgcg
//
LOCUS       DASPWO010000121         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_20433, whole genome shotgun sequence.
ACCESSION   DASPWO010000121 DASPWO010000000
VERSION     DASPWO010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20433"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VEO00_02710"
     CDS             complement(<1..785)
                     /locus_tag="VEO00_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HYU56948.1"
                     /translation="MRLPRASVTLFLLGSLLALDAPGTALAAAGDPDGTFGDGGKVTT
                     PFVFGQDVARDVVVQPDGKVVVVGDAYNGADSDLGVARYLADGSPDTTFSGDGRATIH
                     FVGQDSANGVALQPDGKIVVVGRTGVGDFGVVRLNTNGSLDSSFSGDGKVGTPGLGAA
                     QDVAVVGGKIVVVGGASPSPGDFVVARYTSTGSPDTTFSQDGIATTDFAGSDVAYGLA
                     VQPDGRIVVAGVTSVNMNFALARYTTTGGLDSTFSNDGKVTTSL"
     gene            complement(837..1151)
                     /locus_tag="VEO00_02715"
     CDS             complement(837..1151)
                     /locus_tag="VEO00_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HYU56949.1"
                     /translation="MSVTVKLPTILRPKAGGDAQVAAEPGTLRDLLADLERRFPGLTR
                     NVVTDDGNLHRFVNVYLNDEDVRYLASLETQVSDGDTVNILPAVAGGQEPVPASVVAD
                     AP"
     gene            complement(1227..2057)
                     /gene="moeB"
                     /locus_tag="VEO00_02720"
                     /pseudo
     CDS             complement(1227..2057)
                     /gene="moeB"
                     /locus_tag="VEO00_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
     gene            complement(2054..2452)
                     /locus_tag="VEO00_02725"
     CDS             complement(2054..2452)
                     /locus_tag="VEO00_02725"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HYU56950.1"
                     /translation="MIEIARDLVEEIVSHARAEYPNECCGLLAGEDGRAVRVYRMTNA
                     DASTASYRLDPKEHLEVFDELEEKRWDLLAIYHSHTHSPAYPSETDRKLAFYPGSRYL
                     IVSLAGGEAPDLRAFRIDDGEVAEEEVRVT"
     gene            complement(2476..3525)
                     /gene="selD"
                     /locus_tag="VEO00_02730"
     CDS             complement(2476..3525)
                     /gene="selD"
                     /locus_tag="VEO00_02730"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HYU56951.1"
                     /translation="METETVRLTSFSHGAGCACKLAPADLADVMGRLTHPARPPEVLV
                     DADTGDDAAVYALPDGRALIQTVDFFTPVVDDAYDWGRVAAANALSDVYAMGGAPVLA
                     LNLVAWPVQDLPLELLARVLEGGQAVAAEAGVAIVGGHSIHDPEPKYGMAVTGFADRD
                     GVVRNSTAPPGATLFLTKPLGVGIVTTAIKRGAATPAQARAAVETMAELNRDAAQAML
                     GGGVAAATDVTGFGLLGHLHGMLRASGIAAEVDAGALRFLDGTADLVLRGVVPGGTKA
                     NHRFLNGKVNWGDLVLPEQLMLADAQTSGGLLIAAPDAARMRAALGERGIAAMEIGVT
                     NEGEPGTIRVRGHPR"
     gene            3566..>3768
                     /locus_tag="VEO00_02735"
     CDS             3566..>3768
                     /locus_tag="VEO00_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYU56952.1"
                     /translation="MREEMVEIPVEGAGPLPCFLARADDFPNPSVVVVQEWWGLNDQI
                     RGVAHRLAGERYTAIVPDLYRGAV"
BASE COUNT          514 a         1355 c         1342 g          557 t
ORIGIN      
        1 aggctggtcg tcaccttgcc gtcgttcgag aacgtcgagt ccaggccgcc ggtcgtcgtg
       61 tagcgcgcca gcgcgaagtt catgttcacg ctggtgacgc cggcgaccac gattcgtccg
      121 tcgggctgca ccgccagccc gtaggccacg tcgcttcccg cgaagtcggt ggtggcaatg
      181 ccgtcctgcg agaaggtggt gtcgggcgat ccggtcgacg tgtagcgggc gacgacgaag
      241 tcgcccggcg acgggctggc gcctccgacg acgacgatct tcccgccgac gacggccacg
      301 tcctgtgccg cacccagccc cggcgtaccg accttcccgt cgccggagaa ggagctatcc
      361 agggacccgt tcgtgttcag gcgaacgacg ccgaagtccc cgaccccggt gcgccccacg
      421 accacgatct tcccgtcggg ctggagcgcc acgccgttcg cgctgtcctg gccgacgaag
      481 tggatcgtcg ccctcccgtc gcccgagaac gtcgtgtccg gggacccgtc ggccaggtag
      541 cgggcgacgc ccaggtcgga gtccgcgccg ttgtaggcgt cgccgaccac gaccaccttc
      601 ccgtccggct gcaccaccac gtcgcgcgcg acgtcctggc cgaacacgaa cggcgtggtg
      661 accttcccgc cgtccccgaa cgttccgtcg ggatccccgg cagcggccag cgcggttccg
      721 ggcgcatcga gcgcgaggag cgagccgagg aggaacaggg tgacgctggc acgggggagc
      781 cgcatcccca tcctccagta cgaggggagc agtctaggtt tgtccgattc gcccggtcaa
      841 ggggcgtcgg cgaccaccga agcagggacc ggctcctggc cgccggcgac ggccgggagg
      901 atgttcacgg tgtcgccgtc ggaaacctgc gtctcgagcg aggcgaggta gcggacgtcc
      961 tcgtcgttga ggtagacgtt gacgaaccgg tgcaggttgc cgtcgtcggt gacgacgttg
     1021 cgggtcaggc cggggaaccg ccgctcgagg tcggccagca ggtcgcgaag cgtcccgggc
     1081 tcggcggcca cctgggcgtc gccgcccgcc ttcgggcgca ggatcgtcgg cagcttcacg
     1141 gtcacggaca tcgtgtcctc gctcctcggt cggggtcagg cgttcgccgt cgtcgtcccg
     1201 tgctcggcct ccggcggggc ctggccggga gcgagcccgc agaactggtc gtagtcgatc
     1261 agctcggtga cggtggggtg cttgccgcac accgggcact ccgggtccca gcgcagcttc
     1321 accttcgtga agtccatctg cagcgcgtcg aagatcagca ggctcccctc gagcgtcttc
     1381 ccggcggcgg tgatcagctt gatcgcctcg gtcgcctgga tcgagccgac caggccgggc
     1441 agcacgccga acacgccgcc ctcggcgcac gacgggacgg tccccggcgg gggcggctcc
     1501 gggaacaggc agcggtaaca gggcgtctcg ggccccggca tgaacacgct ggcctgcccc
     1561 tcccactggt agatgcttcc gtacacgagg ggcttgccga gcatctgcgt ggcgtcgttc
     1621 acgaggtagc ggacggggaa gttgtcggtg ccgtccacca ccaggtcgta gggctccagg
     1681 atctcgaacg cgttcgccga cgtgagcggc acgccgtgcg ggatcacctc gacgttcggg
     1741 ttcatcgccg cgatacgctg gcgcgccgag tcgaccttcg gtttcccgac gtcggcggtg
     1801 tcgtgcagga tctggcgctg caggttcgtc aggtcgactc ggtcgaagtc gacgatgccg
     1861 agcgtgccga cgccggcagc ggcgaggtac agggccaccg gcgagccgag cccgccggca
     1921 ccgaccacga gcaccttcgc gtccatcaac ttgcgctgcc ccgccccgcc gatgccggac
     1981 aggatgatgt gccgcgagta gcgcgcgacc tgctcctcgg tgaacagcgg cgccgggcgg
     2041 ccgtcggacg ggctcacgtg acccgcacct cctcctcggc cacctcgccg tcgtcgatcc
     2101 ggaacgcgcg caggtccggc gcttcgccgc cggccagcga gacgatcagg tagcggctcc
     2161 ccgggtagaa cgccagcttg cgatcggtct cggaggggta ggccggcgag tgcgtgtgcg
     2221 agtggtagat cgccagcagg tcccacctct tctcctcgag ctcgtcgaac acctcgaggt
     2281 gctccttcgg gtcgagtcgg taggacgcgg tgctggcgtc ggcgttcgtc atccggtaga
     2341 cgcgcacggc gcggccgtcc tcgcccgcca gcaggccgca gcactcgttg gggtactcgg
     2401 cccgggcgtg ggagacgatc tcctcgacca ggtcgcgcgc gatctcgatc aaccgtgcct
     2461 ctctcgttcg ggatcctacc gagggtgccc gcgaacacgg atcgtgccag gctcgccctc
     2521 gttcgtaacg ccgatctcca tcgccgctat tccccgttct ccgagcgcgg cgcgcatgcg
     2581 ggcggcgtcg ggggcagcga tgagcagtcc gccggaggtc tgggcgtcgg ccagcatgag
     2641 ctgctccggc agcaccaggt cgccccagtt gaccttcccg ttcaggaagc gatggttcgc
     2701 cttggtcccg cccgggacca cgccgcgcag cacgaggtcc gcggtcccgt ccaggaaccg
     2761 cagcgcgccc gcatccacct cggcggcgat gcccgaggct cggagcatgc cgtgcaggtg
     2821 gccgagcagg ccgaaccccg tgacgtcggt tgccgccgcg acgccgcccc cgagcatcgc
     2881 ctgggcggcg tcccgattca gctccgccat ggtctcgacg gccgcgcgcg cctgcgccgg
     2941 ggtggccgcg ccgcgcttga tcgccgtcgt gacgatcccg acgccgagcg gcttggtcag
     3001 gaagagcgtc gccccgggcg gcgccgtcga gttgcgcacc acgccgtcgc ggtcggcgaa
     3061 gccggtcacc gccatcccgt acttgggctc ggggtcgtgg atcgagtggc cgccgacgat
     3121 cgccacgccg gcctccgcgg caacggcctg gccgccctcc agcacgcgcg ccagcaactc
     3181 gaggggcagg tcctgcaccg gccaggccac caggttcagc gccagcaccg gcgcgccgcc
     3241 catcgcgtag acgtccgaca gcgcgttcgc ggcggcgacc cgcccccagt cgtaggcgtc
     3301 gtcgacgacc ggggtgaaga agtccacggt ctggatcagc gcgcggccgt cgggcagcgc
     3361 gtacaccgcc gcgtcgtcgc cggtgtcggc gtccaccagg acctccggcg gacgggccgg
     3421 gtgcgtcagg cggcccatca cgtcggccag gtcggccggc gccagcttgc aggcgcaccc
     3481 cgcgccgtgg ctgaaggagg tgagccggac ggtctcggtc tccacgcagt gaggatacgc
     3541 ggaccgcggg gtacgctccc ccgcgatgcg cgaggagatg gtcgagatcc cggtcgaggg
     3601 tgccggcccg cttccctgct tcctggcccg cgccgacgac ttcccgaacc cgagcgtcgt
     3661 ggtggtgcag gaatggtggg ggctgaacga ccagatccgc ggcgttgccc accgcctggc
     3721 cggcgagcgc tatacggcga tcgtccccga cctgtaccgg ggagcggt
//
LOCUS       DASPWO010000122         3021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_787513, whole genome shotgun sequence.
ACCESSION   DASPWO010000122 DASPWO010000000
VERSION     DASPWO010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787513"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /gene="menC"
                     /locus_tag="VEO00_02740"
     CDS             <1..864
                     /gene="menC"
                     /locus_tag="VEO00_02740"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HYU56953.1"
                     /translation="APEDVGPRLAWARGHRMAKAAVEMAVLDAALSAQGVPLAEHLGA
                     DRDRVPCGVSVGIPGSVDDLLARVGAYLDAGYRRAKLKIEPGWDVEPVRAVREAFPDA
                     HLSVDANAAYRLEDAAIFEALDGLGLSMIEQPLHHEDLWDHARLQARLATPVCLDESI
                     RSAADAALAIELGACRVINVKAGRVGGLLEAVRVRDVAVEAGVLVWCGGMLETGIGRA
                     ANLALAALPGFTMPGDTSASDRYFADDLTEPFVLAPDGTMAVPRGPGIGVEPRPDRLE
                     ACTVRTERITA"
     gene            complement(854..2350)
                     /locus_tag="VEO00_02745"
     CDS             complement(854..2350)
                     /locus_tag="VEO00_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYU56954.1"
                     /translation="MDPEPHRDHFARRASVAAAALVVLASVGALVAVRRPAPGAPAAR
                     TPSPVSPLPRSLRPNLLFVNSDDQRSDTLEVMPKTLRWFNLRFDNAFVTTPLCCPSRA
                     SALTGLYEHHTHVSINNHDAFQPLEPESLGPWLQSLGYRTGFVGKYFNALNRDVKVPQ
                     GWDEFHALVWDNVGHDIGDRYTTFVLREAWTDNGGSKRQRLVAYPNRRFPAAYSTDVF
                     AALAVQFLERAARDRAAGDDRPWALLVWPNAPNGRTPAARHADAPLPPWDPPPSFLET
                     DMSDKPAEVTDGRYVRDTRAFHERIRGVQLRMVMGVDDLVDRVFERIEDDGAIDSTWA
                     VYTSDNGRLWGEHRLWQKLLAYEESVRVPFLMRVPGETRRRFDEIVANIDIAPTLVDL
                     AGDPRDHGFDGRSLVPLVTGRSGRWRTALLLEHEGRAERYTAVRTSRWKLIRWTDSGH
                     EELYDLRADPYELANVAVSNPAEVERLVRLMGELRRSGTPRPVPVPTP"
     gene            complement(2340..>3021)
                     /locus_tag="VEO00_02750"
     CDS             complement(2340..>3021)
                     /locus_tag="VEO00_02750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU56955.1"
                     /translation="LPVPYTNAATAARIPAHEADRWVAETAELFREHRVPAIWWVGPL
                     SEPKHLRAVLVRHGFVHDEEMPWLAADLEVEDLDAPGPRGLVVEAVDGPARQAAWLEV
                     MTAGFGMNEAERESMTRLAAAVGYGPEARWRRFVGFLDGRPVGTSGVMLGGGVAGIYN
                     VATIPGALRRGIGTAMTRVAMRAARDLGYRVAVLGSSQAGMSIYAALGFRDVCTLSLY
                     RWPGPDGP"
BASE COUNT          407 a         1099 c         1121 g          394 t
ORIGIN      
        1 gcgccggagg acgtcggccc ccgcctggcg tgggcgcgcg ggcatcgcat ggcgaaggcc
       61 gccgtcgaga tggccgtcct cgacgcggcg ctctccgcgc agggggtccc cctggccgag
      121 cacctcgggg ccgaccggga ccgcgtcccg tgcggcgtct cggtcggcat ccccggctcg
      181 gtggacgacc tcctggcacg ggtcggcgcg tacctggacg ccggctaccg gcgggcgaag
      241 ctgaagatcg agccgggctg ggacgtcgag cccgtccgcg ccgtcaggga ggcgttcccc
      301 gatgcgcacc tctccgtgga tgcgaacgcc gcgtaccggc tggaggacgc cgcgatcttc
      361 gaggcgctcg acgggctggg gctctcgatg atcgagcagc cgctccacca cgaggacctc
      421 tgggaccatg cccggctgca ggcccgcctg gccacccccg tgtgcctgga cgagtccatc
      481 cgctcggcgg cggacgcggc gctcgccatc gagctgggag cgtgccgcgt gatcaacgtc
      541 aaggccgggc gggtcggcgg gctgctcgag gcggtccggg tgcgcgacgt tgccgtggag
      601 gccggcgtgc tggtctggtg cggcgggatg ctcgagaccg ggatcgggcg ggcggcgaac
      661 ctggcgctcg cggcgctgcc ggggttcacc atgcccggcg acacgagcgc cagcgaccgc
      721 tacttcgccg acgacctgac cgagccgttc gtcctcgcgc ccgatggaac gatggcagtg
      781 ccgcgcggac cgggcatcgg ggtcgagccg cgcccggatc ggctcgaagc ctgcacggtg
      841 cgaaccgaac ggatcacggc gtagggaccg ggaccgggcg aggcgtcccc gaccggcgga
      901 gctcccccat caggcgcact aggcgctcga cctcggccgg gttcgacacg gccacgttcg
      961 ccagctcgta cgggtcggcg cgcaggtcgt agagctcctc gtgccccgag tccgtccagc
     1021 ggatcagctt ccagcgcgac gtgcgcaccg ccgtgtagcg ctcggcccgc ccctcgtgct
     1081 ccagcagcag ggccgtccgc caccggccgc tccgcccggt gacgagcggc accagtgacc
     1141 gcccgtcgaa cccgtggtcg cgcgggtcgc cggccaggtc cacgagcgtc ggcgcgatgt
     1201 cgatgttcgc cacgatctca tcgaagcggc gtcgcgtctc gccggggacg cgcatcagga
     1261 aaggcacccg cacgctctcc tcgtaggcca gcaacttctg ccacagccgg tgctcacccc
     1321 acaggcggcc gttgtccgag gtgtacaccg cccacgtcga gtcgatcgcg ccatcgtcct
     1381 cgatccgttc gaagacccgg tcgacgaggt cgtccacgcc catcaccatc cgcagctgaa
     1441 cgcctcggat gcgctcgtgg aacgcgcggg tgtcccgcac gtagcggccg tccgtgacct
     1501 ccgccggctt gtcggacatg tcggtctcca ggaaggaggg cggcgggtcc cagggcggaa
     1561 gcggcgcgtc ggcgtggcgc gcggccgggg ttcggccgtt cggcgcgttc ggccagacga
     1621 gcagcgccca cggacggtcg tcgcccgcgg cccggtcacg cgcggcgcgc tccaggaact
     1681 gcacggcgag cgcggcgaac acgtcggtcg agtaggcggc cgggaaccgg cggttcgggt
     1741 acgccaccag gcgctggcgc ttgctccctc cgttgtcggt ccacgcctcg cgcaggacga
     1801 acgtcgtgta gcggtcgccg atgtcgtgtc cgacgttgtc ccacacgagc gcgtggaact
     1861 cgtcccaccc ctgtgggacc ttgacgtcgc ggttcagtgc gttgaagtac ttcccgacga
     1921 acccggtgcg gtagccgagc gactgcagcc acggtccgag cgactccggc tcgagcggct
     1981 ggaacgcatc gtggttgttg atcgagacgt gcgtgtggtg ctcgtagagg ccggtcaggg
     2041 cgctggcacg ggacggacag cagagcggcg tggtgacgaa cgcgttgtcg aagcggaggt
     2101 tgaaccaccg cagcgtcttc ggcatcacct cgagggtgtc ggagcgctgg tcgtcggagt
     2161 tcacgaacag caggttgggg cgcaggctgc gagggagcgg cgagaccggg ctcggggtcc
     2221 gcgcggccgg ggcgcccggc gccggacgcc gaacggccac cagcgccccg acggacgcga
     2281 ggacgaccag cgccgccgcc gcgaccgacg cccggcgcgc gaagtggtcc cggtgcggct
     2341 cagggtccat cgggcccggg ccagcggtac agcgacagcg tgcagacgtc ccggaacccg
     2401 agggcggcgt agatgctcat gccagcctgg gaggagccga gcacggccac gcggtatccg
     2461 agatcgcggg ccgcccgcat ggccacccgg gtcatcgccg tcccgatgcc tcggcggagc
     2521 gccccgggga tcgtcgccac gttgtagatg ccggcgacgc cgcccccgag catcaccccc
     2581 gatgtcccca ccgggcggcc gtcgaggaac ccgacgaacc ggcgccatcg cgcctcgggc
     2641 ccgtagccga ccgccgcggc cagccgggtc atcgactccc gctcggcctc gttcatgccg
     2701 aagccggccg tcatcacctc gagccacgcc gcctgccgcg ccgggccgtc gacggcctcg
     2761 acgaccaggc cccgcgggcc gggggcgtcg aggtcctcga cctccagatc ggccgcgagc
     2821 cacggcatct cctcgtcgtg gacgaacccg tgacggacga gcaccgcgcg gaggtgcttc
     2881 ggctcggaca gggggccgac ccaccagatg gccgggacac ggtgctcgcg gaacagctcg
     2941 gcggtctccg cgacccagcg gtccgcctcg tgtgcgggga tgcgggcggc ggtcgccgcg
     3001 ttcgtgtacg gcaccggcag c
//
LOCUS       DASPWO010000123         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_951401, whole genome shotgun sequence.
ACCESSION   DASPWO010000123 DASPWO010000000
VERSION     DASPWO010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951401"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VEO00_02755"
     CDS             complement(<1..968)
                     /locus_tag="VEO00_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HYU56956.1"
                     /translation="MERRRICVDGNEAAALVAYALSEVIAVYPITPASPMGEHADDWA
                     AAGRPNLWGVVPDVVEMQSEAGAAGALHGALQKGALATTFTASQGLLLMVPNMFKIAG
                     ELTPTVIHVAARTVATHALSIFGDHSDVMHARTTGWAMLCSSSVQEAHDFALIAHAAT
                     LRSRVPFLHFFDGFRTSHEIDRLEALGDDDLRAIVSDDDILEFRGRGLTPDAPVLRGT
                     AQNPDAFFQAREACNPFYLAAPGIVRGVMGSFGERTGRRYDLVEYTGPPDAERVVVLM
                     GSGVGAAEEAVQHLGSRGEPVGLLTVRLYRPFPAEQLVAALPPSVRT"
     gene            complement(1084..2322)
                     /locus_tag="VEO00_02760"
     CDS             complement(1084..2322)
                     /locus_tag="VEO00_02760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HYU56957.1"
                     /translation="MASLLSVIEDLLGPDLPVAIRAFDGSRVGPTDPPATVVIRSRRA
                     LQRIVTAPGELGIGRAYVAGELDVEGDIFEALSAIRARLPTVRLGLRHWAALARLVGP
                     AALRPPPRPAEEARLRGRRHSRERDAAAIAHHYDVSNAFYRMVLGPSMTYSCAVWADP
                     RVSLEEAQAAKHELVCRKLDIRPGTRLLDVGCGWGGMAMHAASRHEARVVGVTISTRQ
                     AEWARKAVAEAGLADRVEIRLQDYRDVRDGPFDAISSIGMFEHVGLSQLGVYFRRLFS
                     LLRPGGRLLNHGISRPPSRRARFSRHSFIDAYVFPDGELHEVGSVVSAMQRAGFEARH
                     VESLREHYALTLRAWVGNLEANWEAAVAEVGTARARIWRLYMAASALNFEAGSTQVHQ
                     VLAVRHVAGESGLPLRPEFT"
     gene            2403..2540
                     /locus_tag="VEO00_02765"
     CDS             2403..2540
                     /locus_tag="VEO00_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56958.1"
                     /translation="MDRRKMVDAYVLIQVEPGTAGRGVSAEVAIVHGILAADDVSAPT
                     T"
BASE COUNT          435 a          989 c         1040 g          409 t
ORIGIN      
        1 gtacgcaccg agggagggag ggcggcgacc agctgctcgg cggggaaggg ccggtacagc
       61 cggaccgtga gcagccccac cggctcaccc cgcgagccga gatgttggac ggcctcctcc
      121 gccgcgccga cgcccgagcc catcaggacc acgactcgct cggcgtcggg gggcccggtg
      181 tactcgacca ggtcgtaccg cctcccggtc cgctcgccga acgatcccat gaccccccga
      241 acgatcccgg gcgccgccag gtagaacgga ttgcaggcct ccctggcctg gaagaaggcg
      301 tcggggttct gggcggtgcc gcggaggacc ggggcgtcag gagtgagtcc gcgcccgcgg
      361 aactccagga tgtcgtcgtc ggagacgatc gcccgcaggt cgtcgtcgcc gagcgcctcg
      421 agccggtcga tctcgtggga ggtccggaag ccgtcgaaga agtgcaggaa cggtacccgg
      481 gagcgaagcg tggccgcgtg agcgatcagg gcgaagtcgt gcgcctcctg cacgctcgag
      541 gagcacaaca tggcccatcc tgtggtccgg gcgtgcatga cgtcgctgtg gtccccgaag
      601 atggacaggg catgggtggc gacggtgcgg gccgccacgt ggatcaccgt cggggtcagc
      661 tcgcccgcga tcttgaacat gttcgggacc atcaacagca ggccctgcga cgcggtgaac
      721 gtcgtggcga gggctccctt ctgcagggcc ccgtgcaggg cgccggccgc gcccgcctcg
      781 gactgcatct ccacgacgtc ggggaccacg ccccacaggt tcggtcgccc ggcggccgcc
      841 cagtcgtctg cgtgctcgcc catgggcgag gccggcgtga tcggatagac ggcgatgacc
      901 tcgctcagcg cgtaggcgac cagggccgcc gcctcgttgc cgtcgacgca gatccggcga
      961 cgttccactc gctcctcacc cctttcctgc acgatggaga tcacgtgggg ccttccgccc
     1021 aaccgtacgc cttggccccg cggcgcgagc gaggtcggac cgcgccggct acggcccggt
     1081 gcctcaggtg aactcggggc ggagggggag gccgctttcg ccggcaacat gtcggaccgc
     1141 gaggacctgg tggacctgcg tgctgccggc ctcgaagttc aacgccgagg ccgccatgta
     1201 cagccgccag atgcgggctc gagccgtgcc cacctcagcg accgctgcct cccagttggc
     1261 ctcgaggttg cccacccacg cgcgcagcgt gagcgcgtag tgctcgcgca ggctctcgac
     1321 gtgccgcgcc tcgaagcccg cgcgctgcat cgcggagacc acgctgccga cctcgtgcag
     1381 ctcgccgtcg gggaagacgt aggcatcgat gaagctgtgc cgggagaatc gggcccgacg
     1441 gctcggcggt cggctgatgc cgtggttgag cagccgccct cccggccgca gcaacgagaa
     1501 cagccggcgg aagtacacgc cgagctggga gaggcccacg tgctcgaaca tgccgatcga
     1561 ggagatggca tcgaaggggc cgtcccgaac gtcccggtag tcctggaggc gaatctctac
     1621 gcggtcggcg agccccgcct cggcaaccgc cttcctggcc cactcggcct gcctggtgga
     1681 gatcgtcacc cccaccaccc ttgcctcgtg tcgcgacgcg gcgtgcatgg ccatgccgcc
     1741 ccacccgcag ccgacgtcga ggagcctcgt gccggggcgg atgtcgagct tgcgacagac
     1801 cagctcgtgc ttggcggctt gcgcttcctc cagggagacg cgggggtcgg cccagacggc
     1861 acaggagtac gtcatcgagg ggccgaggac catgcgatag aaggcattcg acacgtcgta
     1921 gtggtgggcg atcgcggcgg cgtcgcgttc cctggagtgc cgccgtcccc gcagccgtgc
     1981 ctcctctgcc ggccggggtg gcggccggag cgcggccggc ccgaccagcc gggcgagggc
     2041 cgcccagtgc cggagcccca gccggacggt ggggagcctg gcgcggatgg ccgacagcgc
     2101 ctcgaagatg tcgccctcca cgtcgagttc accggccacg taggcgcgac cgatccccag
     2161 ctcgcccggc gcggtcacga tccgttggag cgcccgccgc gaccggatga ccaccgtggc
     2221 cggcgggtcc gtgggtccga cccggctccc gtcgaaggcg cggatcgcga cggggagatc
     2281 cggacccagg agatcctcga tgacggagag gaggcttgcc acgaaggcga ctctatgtcg
     2341 ccgcggagct gagggcttgc gtacgtctgg ccaatccgac cgtaggattc gtctcggcgg
     2401 ggttggacag gaggaagatg gtcgacgcgt atgtgctgat ccaggtcgaa ccgggcacgg
     2461 ccggcagggg cgtctccgcg gaggtcgcca tcgtccacgg aatcctggcc gcagacgacg
     2521 tctcggcccc tacgacgtga tcgcacgagc tcaggcgtcg agtctcgatc acctcatgaa
     2581 ggccgttgtg gcgaggattt aggccatcga cggcatcatt cgtacgctcg tttgccccgt
     2641 ggtccgcttg agctagggct cgagccgtcg acgacgctcg agatccgctg cgatgtccgg
     2701 catgaggcgc cagcatcccg tcgcggtcct acgactccgg gaggtgcacg gacacgacca
     2761 caccggcgtg gtcggagggc cgcagcggcg tcgtggagga cgaagtcgac catgttgtcg
     2821 agggtcgagg gcaacgtgca gtccaggtcg tcgggctgcc cggccgtgta ggc
//
LOCUS       DASPWO010000124         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_612436, whole genome shotgun sequence.
ACCESSION   DASPWO010000124 DASPWO010000000
VERSION     DASPWO010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612436"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VEO00_02770"
     CDS             complement(<1..327)
                     /locus_tag="VEO00_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYU56959.1"
                     /translation="MTERTGHPDELLAGYVDGDLAGDEREAVETHLAGCATCREEVAL
                     ARRAVAALEALEPAPSPLGLGTPAILGARTTPRRGVLRPRWAAALAAAAVVVVAAAVG
                     GRFLIQG"
     gene            complement(324..920)
                     /locus_tag="VEO00_02775"
     CDS             complement(324..920)
                     /locus_tag="VEO00_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU56960.1"
                     /translation="MENGPSDEELVRRYRAGDGGAFTDLVRRHEPRVYNLALRMLGRA
                     EDARDAAQDTFLTVLRKLDGFRGDARFTTWLYRVTSNTCYDLLRKRRREGPATDELPE
                     TAAPDDPESAAIARADVARAIGKVPEEFRLVLLLHDVEDLGHAEIASIVGVPIGTVKS
                     RLHRGRVALGRLLSDVSGRDEAGGTPVRRAPSKHGEDR"
     gene            complement(923..2416)
                     /locus_tag="VEO00_02780"
     CDS             complement(923..2416)
                     /locus_tag="VEO00_02780"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYU56961.1"
                     /translation="METRPAGERLAGRYVLEEPIAAGGMATVWRARDEVLARPVAVKI
                     LREDLAESEAFRERFHREAVAAARLSHPRIVAVYDTGGTGDTSFIVMELFDGTSLADV
                     LRNGPLKPRRAAQIVVAALEGLGYAHGEGLVHRDVKPANILVAGDGRVKVTDFGVAKA
                     AFDDHDLTSTGKVLGTVRYLSPEQVEGGTADARSDVYAAGVVLYEALTGRPPFQAEND
                     LATAMLRLRQDPMPPGAVRAGIPRALEAVTMRALARRPEDRYASAEEMRLDLVRALGP
                     GGHPVTTEIRPSAPPPPRGVEHDREPGMFRTWMLVPLIAVLVAAALIVVGLVLGRLEI
                     GGPLGVRPAENAGNGNGTVSIRPATIVDFDPPPGDLHENTQDVPLVADGDPATVWRTE
                     GYNSADLGRLKPGVGLLFDLGAQRSVSRVVVTSPLPGWTFEIRAADVQGAVLQGSPKA
                     SLTAGDKVQADAEFEARYVLIWITRLAPSEDRFRAEISEVRFLGPEA"
     gene            complement(2427..3251)
                     /locus_tag="VEO00_02785"
     CDS             complement(2427..3251)
                     /locus_tag="VEO00_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HYU56962.1"
                     /translation="MIAVVTDSAADLPLDLAARHGVRVVPLTVAFGTEALDDGTGLPP
                     ETFWDRIAAGAGVPTTASPPPEALARAYRAAAEEGAEGVVSIHLSRALSRTVETAAAA
                     GRDAPIPVEVIDSASVSLGQGLVVLAAAAAGAGGSVGEASAAARRAVAGIEVFAVLET
                     VDFLRRGGRVGAAKAALSDLLRIRPVLTLAGGQTSLVARARTRSRAIAEVLERTRGPA
                     EAAGVLHSRAPEVEDVARAVREACGVEPIVSLIGAVTGSHLGPGAIGVVVLRPSSP"
     gene            complement(3319..3717)
                     /locus_tag="VEO00_02790"
     CDS             complement(3319..3717)
                     /locus_tag="VEO00_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56963.1"
                     /translation="MRPRLTDDSGLILKWFARLLIVFAILGVAAVDGISILFAHLRAD
                     DAAAAGAIACAAKYRDTGNAQTAETEARDAVTQKDETIKVTTFFINPQTGECTVRTRE
                     VATTLVVGRVGFLKKWGIAKAEETATPPTA"
     gene            complement(3714..>4765)
                     /locus_tag="VEO00_02795"
     CDS             complement(3714..>4765)
                     /locus_tag="VEO00_02795"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HYU56964.1"
                     /translation="PGPAVPTPDGITTLQRYVLAIGTTAGVLAMTVALWPSLRRIGFR
                     FRFTPHLRHPAVRRIARLAGWSLVYVAVNQVGLLVTVVLAGTERGAYTAYALAFIFFQ
                     LPYAIFAVSVMTALLPRMSESWVERDRGEFGADLAQGIRATAFVVLPAAAGYVAVSQP
                     IVRLLLQHGVTTPRSTELVARVLVAFSVGLFPFAAFQLLLRAFYAMQDTRTPALVNVG
                     AEAVHLAANLTLFLGLGLGVQGLALGHAAGYVFAALVAAVLLARRVGGLRARSLLAGL
                     WKIALASALTGLAAWLAARGIGTWLGTGSLVAQLAQVAGAVLAGLIIFATVSIALRVE
                     EFGLVKRFVLARLGR"
BASE COUNT          663 a         1777 c         1770 g          555 t
ORIGIN      
        1 gccttggatc aggaagcggc cgccgacggc cgcggccacc acgacgacgg ccgcggcggc
       61 gagtgccgcc gcccagcgcg ggcgaaggac gccgcggcgg ggggtggtgc gcgccccgag
      121 gatggcgggg gtgccgagcc cgagcgggga gggggccggc tcgagcgcct cgagcgccgc
      181 cacggcgcgc cgcgccagcg ccacctcctc gcggcaggtc gcgcagccgg cgaggtgcgt
      241 ctcgacggcc tcgcgctcgt cgcccgccag gtcgccgtcc acgtaccccg ccagcagctc
      301 gtcgggatgc ccggtccgct ccgtcatcgg tcctctccgt gcttcgacgg cgcccgccgg
      361 accggggttc ccccggcctc gtcccggccg gacacgtccg acagcaggcg gccgagcgcg
      421 acccgcccgc ggtgcagccg cgacttcacc gtcccgatcg gcacgccgac gatcgaggcg
      481 atctctgcgt ggccgaggtc ctccacgtcg tgcagcagga gcaccaggcg gaactcctcg
      541 gggaccttcc cgatcgcgcg cgcgacgtcg gcccgggcga tggcggcgga ctcggggtcg
      601 tccggggcgg ccgtctctgg cagctcgtcg gtggccggtc cctcgcgccg acgcttgcgc
      661 agcaggtcgt agcaggtgtt cgaggtgacg cggtagagcc acgtggtgaa ccgcgcgtcg
      721 ccgcggaagc cgtcgagctt gcgcagcacg gtcaggaacg tgtcctgcgc cgcatcgcgg
      781 gcgtcctcgg cccggccgag catgcgcagc gcgaggttgt agacgcgggg ctcgtggcga
      841 cggaccaggt cggtgaacgc cccgccgtcc cccgcccggt agcgccggac gagctcctcg
      901 tcggacggcc cgttctccac gctcacgcct cggggccgag gaagcggacc tccgagatct
      961 cggcccggaa gcggtcctcc gagggcgcca gccgtgtgat ccagatcagg acgtatcgcg
     1021 cctcgaactc ggcgtccgcc tgaaccttgt ccccggcggt cagcgaggcc ttcggcgagc
     1081 cctgcagcac cgccccctga acgtcggcgg cccggatctc gaacgtccag ccgggcaggg
     1141 gactcgtgac gaccacacgg gagaccgagc gctgcgcgcc gaggtcgaag agcagcccga
     1201 cgccgggctt caggcggccg aggtcggcgc tgttgtagcc ctcggtcctc cacaccgtgg
     1261 ccgggtcgcc gtcggccacg agcggcacgt cctgggtgtt ctcgtgcagg tcgcccggcg
     1321 gaggatcgaa gtcgacgatc gtggcgggcc ggatggacac cgtcccgttc ccgttgccgg
     1381 cgttctcggc gggacgaact ccgagcgggc cgccgatctc gagccggccg agcaccaggc
     1441 cgacgacgat cagtgcggcg gccacgagca ccgcgatcag cggcaccagc atccacgtgc
     1501 ggaacatccc gggctcgcgg tcgtgctcga cgccccgcgg gggaggcggc gcggagggcc
     1561 ggatctcggt ggtgaccgga tggccgcccg ggcccagggc gcgcaccaga tcgagccgca
     1621 tctcctcggc gctcgcgtag cggtcctccg gccggcgcgc cagcgcgcgc atcgtgaccg
     1681 cctcgagcgc ccgcgggatc ccggcacgga ccgcacctgg cggcatcggg tcctgccgca
     1741 atcggagcat ggccgtggcc aggtcgttct cggcctggaa cggcggacgc ccggtgagcg
     1801 cctcgtacag gacgaccccg gcggcgtaga cgtcgctccg ggcgtcggcc gtcccgccct
     1861 cgacctgctc gggcgagagg tagcggacag tgccgagcac cttgccggtc gaggtcaggt
     1921 cgtggtcgtc gaaggccgcc ttcgccacgc cgaagtcggt gaccttcacc cggccgtcgc
     1981 cggcgaccag gatgttcgcc ggcttgacgt cgcggtggac caggccttcc ccgtgcgcgt
     2041 acccgaggcc ctcgagggcg gccaccacga tctgggcggc ccgccgcggc ttgagcgggc
     2101 cgttccgcag gacgtcggcg aggctcgtcc cgtcgaacag ctccatgacg atgaacgacg
     2161 tgtcgcccgt accgccggtg tcgtacacgg cgacgatgcg ggggtgggag agccgcgcgg
     2221 cggcgaccgc ctcgcggtgg aatcgctcgc ggaaggcctc gctctcggcc aggtcctcgc
     2281 ggaggatctt gacggccacc gggcgggcga gcacctcgtc ccgcgcccgc cagacggtgg
     2341 ccatgccgcc cgcggcgatc ggctcctcga ggacgtagcg gccggcgagg cgctcgccgg
     2401 cgggccgcgt ctccatgtcg ccgagtctat ggggaggagg ggcggagcac gacgacgccg
     2461 atggcgccgg gcccgaggtg cgagccggtg acggcgccga tcagcgagac gatcggctcg
     2521 accccgcagg cctcgcggac cgcgcgggcc acgtcctcga cctccggcgc ccgggagtgg
     2581 agcacgccgg ccgcctcggc cggcccgcgc gtccgctcca acacctcggc gatcgcccgc
     2641 gaacgggtgc gggcacgggc gacgagcgac gtctggccgc cggcgagcgt cagcaccggg
     2701 cggatgcgca gcaggtcgga aagggccgcc ttcgccgctc caacccgccc gccacggcgc
     2761 aggaagtcga ccgtctcgag cacggcgaac acctcgatcc cggccacggc ccggcgagct
     2821 gcggcggatg cctcccccac ggaaccgccg gcgcccgccg cggccgccgc gagcacgacg
     2881 agcccctggc ccagggagac cgaagccgag tcgatcacct cgacggggat cggggcgtcc
     2941 ctcccggccg cggccgcggt ctcgacggtg cgcgacagcg cgcgcgacag gtggatggac
     3001 accacgccct cggcaccctc ctcggcggcc gcgcggtacg cgcgggccag ggcctcgggc
     3061 ggcggtgagg cggtggtcgg gacgccggcg ccggcggcga tccgatccca gaacgtctcg
     3121 ggcggcaggc cggtcccgtc gtcgagcgcc tcggtcccga acgccacggt gagcgggacg
     3181 acgcgcaccc cgtgtcgagc ggcgaggtcc aggggcaggt cggcggcgct gtcggtgacg
     3241 accgcgatca tctggacaag tggggaggag accctcccca ccccccaccc gttctcagca
     3301 cacgggcttc gccggtgctc acgcggtagg gggcgtggcg gtctcctcgg ccttggcgat
     3361 gccccacttc ttcaggaatc cgacgcgccc gacgacgagc gtcgtcgcca cttcccgcgt
     3421 tcgcacggtg cactcgcccg tctgggggtt gatgaagaac gtcgtgacct tgatcgtctc
     3481 gtccttctgc gtcaccgcgt cgcgcgcctc ggtctccgcc gtctgcgcgt tccccgtgtc
     3541 tcgatacttg gcggcgcagg cgatcgcgcc ggcggccgcc gcgtcgtccg cgcgcaagtg
     3601 cgcgaagagg atcgagatgc cgtcgacggc cgcgacgccg aggatggcga acacgatcag
     3661 caggcgggcg aaccacttca ggatcaggcc gctgtcgtcg gtcaggcgcg gcctcatcgc
     3721 ccgaggcgag cgagaacgaa ccgcttcacc agcccgaact cctccacgcg cagagcgatc
     3781 gacacggtgg cgaagatgat aagcccggcg aggaccgccc ccgcgacctg cgccagttgc
     3841 gcgacgagcg accccgtgcc gagccacgtc ccgatgccgc gcgcggcgag ccatgccgcc
     3901 agcccggtca gcgcggaggc gagcgcgatc ttccacagcc cggcgagcag gctccgcgcg
     3961 cgcagccccc ccacccgccg agcgagcagg acggccgcca cgagcgccgc gaacacgtag
     4021 cccgccgcat ggccgagggc gaggccctgg acccccaggc cgagcccgag gaacagcgtc
     4081 aggttcgccg ccaggtggac ggcttccgcg ccgacgttca ccagcgccgg cgtacgggtg
     4141 tcctgcatcg cgtagaacgc ccggagcagc agctggaacg cggcgaacgg gaacagcccg
     4201 acggaaaagg ccaccaggac ccgggccacg agctccgtcg atcgcggcgt ggtcacgccg
     4261 tgctgcagga ggagtcgcac gatgggctgc gataccgcga cgtacccggc ggcggccggg
     4321 agcacgacga acgcggtcgc ccggatgccc tgcgccaggt cggcgccgaa ctcgccgcgg
     4381 tcgcgctcga cccaggactc gctcatccgc ggcaggaggg cggtcatcac cgagaccgcg
     4441 aagatcgcgt acggcagctg gaagaagatg aacgccagcg cgtacgcggt gtaggcgccg
     4501 cgctcggtgc cggccagcac gacggtgacg agcaggccca cctggttcac cgcgacgtac
     4561 acgagcgacc atccggccag gcgcgcgatc cgccgtacgg ccgggtggcg caggtggggc
     4621 gtgaaccgga agcggaaccc gatccgacgc agggacggcc agagcgccac cgtcatcgcc
     4681 aggacgccgg cggtggtgcc gatcgccagc acgtagcgct gcagcgtggt gatcccgtcc
     4741 ggggtgggga ccgccggccc aggga
//
LOCUS       DASPWO010000125         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_951617, whole genome shotgun sequence.
ACCESSION   DASPWO010000125 DASPWO010000000
VERSION     DASPWO010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951617"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..538)
                     /locus_tag="VEO00_02800"
     CDS             complement(74..538)
                     /locus_tag="VEO00_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYU56965.1"
                     /translation="MRIGPGDLVQVMRALHDEPDLRFRFLADLAGVDTGTHLQAVYHL
                     WSDTTADWLRVIVDGLPRDDPRVPSVTFLWKGAEWQEREAYDMFGITFEGNRDLRRIY
                     MPPDYTSFPLRKDFYLADDAARSPAGGVRHMEQVHAPRNAPPAEAVRRTTIA"
     gene            complement(630..1121)
                     /gene="nuoB"
                     /locus_tag="VEO00_02805"
     CDS             complement(630..1121)
                     /gene="nuoB"
                     /locus_tag="VEO00_02805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011270898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HYU56966.1"
                     /translation="MEVIPTKADYVLDLLRANSLWPLLSGLACCAIEMMSAATSVNDM
                     DRWGMFPFRASPRQADVLIVAGTCTTKMAGPLVRLWEQMPEPKWCVAMGDCTCSGGRY
                     KRSYSTIQGIDRIMPVDVYVPGCPPRPEGLIYGMMKLQQIVKDRRGHWPERAVGPTVP
                     DAI"
     gene            complement(1391..1747)
                     /locus_tag="VEO00_02810"
     CDS             complement(1391..1747)
                     /locus_tag="VEO00_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009728220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYU56967.1"
                     /translation="MTAIWYVVGFAFAGVSFVFITIGAAWLLSHRNRGDVHKGLPYES
                     GIDTYGDTHARFGLSFYLYALLFVAFDIEVIFIYLWALVFRDVLMGGFVSMLIFVGIL
                     LLGLAYAWRKGVLTWH"
BASE COUNT          343 a          734 c          783 g          318 t
ORIGIN      
        1 gagcatgatc cgcggatcgt cggcggaggc gtgctggccg gggtggttcg tcgctgtcat
       61 gacgggcggt cggtcaggcg atcgtggtcc gccgcacggc ctcggccggc ggagcgttgc
      121 gcggcgcatg gacctgctcc atgtgacgaa cgccgccggc cggagatcgg gcggcgtcgt
      181 cggcgaggta gaagtccttg cgcaacggga agctggtgta gtcgggcggc atgtagatgc
      241 ggcgcaggtc gcggttgccc tcgaaggtga tcccgaacat gtcgtaggcc tcgcgttcct
      301 gccattcggc gcccttccag aggaaggtca cggacggcac ccgcgggtcg tcccgcggca
      361 ggccatcgac gatgacgcgg agccagtccg ccgtcgtgtc gctccagagg tgatagacgg
      421 cctgcaggtg cgtaccggtg tccacgccgg ccaggtcggc gaggaagcgg aaccggaggt
      481 ccggctcgtc gtggagggcc cgcatgacct gcacgagatc gcccggcccg atgcgcatct
      541 ccgtctcgtc gttacgctgg cggacagggc ctgtcataac ctcgccgaaa cggccgtcca
      601 ggcgacgcct gagcgatcga tccatcgatt cagatcgcgt ccgggacggt gggcccgacc
      661 gcccgctccg gccagtggcc acggcgatcc ttgacgatct gctggagctt catcatcccg
      721 tagatgagac cctccggccg tggcgggcag cccggcacgt agacgtccac cggcatgatc
      781 cgatcgatgc cctggatcgt ggagtagctc cgcttgtagc ggcctccgga gcaggtgcag
      841 tcgcccatgg ccacgcacca cttcggctcc ggcatctgct cccacaggcg gaccaggggc
      901 ccggccatct tggtcgtgca cgtgcccgcg acaatgagga cgtcggcctg gcgggggctg
      961 gcccggaacg ggaacatccc ccagcgatcc atgtcattca cgctggtcgc cgcggacatc
     1021 atctcgatcg cacagcaggc gaggcccgac aggagcggcc agaggctgtt cgcccggagg
     1081 aggtccagca cgtagtcggc cttggttggg atcacctcca gggcgccgcc ccagcgtgcg
     1141 ttgtcgcgag cggcggcgcc cgagcgctcg acgtcggcga gatgggtgat ctccgacggc
     1201 cgcccgccgt cgtaggccag cgggttggtc gattgccaga gcgtgttcgg gacgacgatg
     1261 ggctgaacga catcgcgatt cgcggtcggc gcgggcaggt tggagttgcc ggcccgcttc
     1321 gagttgccgg cccgcttcga gttgccggcc ccggtggggc ctggcttccc gcgggacctg
     1381 gagtcgcggg tcagtgccat gtgagcactc cctttcgcca ggcgtaggcc agacccagaa
     1441 gcaggatccc cacgaagatg agcatgctga cgaacccgcc catgaggacg tcccggaaga
     1501 cgagcgccca gaggtagatg aagatgacct cgatgtcgaa ggcgacgaac agcagggcgt
     1561 agaggtagaa ggacagcccg aaccgggcgt gggtatcgcc gtacgtgtcg atgccgctct
     1621 cgtacggcag gcccttgtgg acgtcgccgc ggttgcgatg ggacagcaac caggctgcgc
     1681 cgatggtgat gaacacgaag gagacgcccg cgaaggcgaa cccgacgacg taccagatcg
     1741 cggtcaagtg aagacctcac tccgagcacg cgtccggcac cgatttcccg ggcgtgcact
     1801 cccttcgggc gcggccagcc ggcgcggcat accgcttcgg cattctagca gcgagcgccg
     1861 cacgagacgc tgccgcggcg gggtgcgagg catcggtcgg cgggccaggt tcgccccatc
     1921 ggatgcgtcg cgatagggag ttcgccctat tgcccggcct gcccgacgcg cgcacgctgg
     1981 ccaggcccga aacgaccggt ggcttaccgc cggggggcga accgccgggg gcaccgccgg
     2041 ggggcggctc gcgaacctgc acgcgcgctc ggcgcgagcc gctccccgac ccgcctcaga
     2101 ccctggccgc cgcggcagcc caccacgcgg cggcccatgc gtcccaccga tcagccgcgg
     2161 atccgataga ccagcccg
//
LOCUS       DASPWO010000126         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1041684, whole genome shotgun sequence.
ACCESSION   DASPWO010000126 DASPWO010000000
VERSION     DASPWO010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041684"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VEO00_02815"
     CDS             complement(<1..311)
                     /locus_tag="VEO00_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HYU56968.1"
                     /translation="MLPHQTSFDDLGAALSEVTFVALDLETTGGSPASCAITEVGAVK
                     MRGGDVLGTFQTLVDPGVPIPRFITHLTGIDDWTVRAAPPLEAVLPSLVEFVAGSVLV
                     A"
     gene            complement(397..663)
                     /locus_tag="VEO00_02820"
     CDS             complement(397..663)
                     /locus_tag="VEO00_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HYU56969.1"
                     /translation="MDEAPSRDERREQVPPPDAWQERAACYGLDPEIFFPTSEEEAGL
                     ALSYCNACSVREICLEWAIQNGERYGVWGGTTEQERRRLMRRVA"
     gene            792..1016
                     /locus_tag="VEO00_02825"
     CDS             792..1016
                     /locus_tag="VEO00_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56970.1"
                     /translation="MEAPIPRTRFRCGSCGNLTRFDVTEHRRTRAYWHFTIEGELTVE
                     DEEVLAADREEVRCRWCGATDGIEEVAVDG"
     gene            1009..2190
                     /locus_tag="VEO00_02830"
     CDS             1009..2190
                     /locus_tag="VEO00_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYU56971.1"
                     /translation="MAEAAATGNGPLAGIRVLDLTRLLPGGYCTLLLADLGADVVKVE
                     EPGRGDYVRWSAPAVDGQSAAHRALNRGKRSITLNLKTPDGVDLLLRLVERFDVLVES
                     YRPGVLDRLGVGHAAVSARNAGIVYCAISGYGQDGPYRDLAGHDVNYLGYAGVLGITG
                     GPDGMPVLPGVQIGDLGGGALLGAAGILAALVDRERTGRGRFVDVAMLDGVFSWLSMH
                     LGNFLADGRVPGPSRMPLNGGYACYRVYRAGDGRHLAVGALEPQFWRGLVEALGRPDL
                     AQEQFAPRQEEIAAELQAIFDEKPRDEWVAALGPLSVCVGPVNDFAEAVRDPQIIARG
                     MVARVDGSPVGPSSPLRFSGTGGPALRPAPELGEHSAELLSEVGVDEAALAELRAAGA
                     V"
     gene            2281..>3482
                     /gene="sepH"
                     /locus_tag="VEO00_02835"
     CDS             2281..>3482
                     /gene="sepH"
                     /locus_tag="VEO00_02835"
                     /inference="COORDINATES: protein motif:HMM:NF022712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HYU56972.1"
                     /translation="MIRIYLVGTTRDGKKLLFARTRGAKFGSMLVPITPQLAKLVAGA
                     QRSPLRTLTRTEPRIVPVAPGPARPLRPAPAPEEPAPEEPVAEGSEPRTIPIPEPGEV
                     IRLPGGARVIRVSPGEFRRLTGLAPPAPEPEPEPEPEPGPAPEAEPGEPAERGAEGAV
                     PETRPTPEPKVRAARSGARARPKARPKARPKAKRKAKPKAEPKPEPKTEPKTEPKTEP
                     PPPKRPEIRSRLSPAQIQALLRRGRSARSVAALAGAPLEWVQRWLEPILSERVGVVGE
                     LKRAWFSKPRLGRSAVPIGVAIEENLRDRGVAFPERVAADGWRAHRPEGGTWRLRFTY
                     HHRGAARSAQWSYDPATRTAAARNRLAAELSWRPPPGATAEATPPKHRRSTGRPRAVR
                     RTATPARR"
BASE COUNT          458 a         1275 c         1338 g          411 t
ORIGIN      
        1 tgcgccacga gcaccgaccc ggccacgaac tcgaccagcg acggcagcac cgcctcgagc
       61 ggcggcgcgg cacgcacggt ccagtcgtcg atgccggtga ggtgcgtgat gaaccgcggg
      121 atcggcacgc ccgggtcgac cagggtctgg aacgtgccca ggacgtcacc gccgcgcatc
      181 ttcaccgcgc cgacctcggt gatcgcgcac gacgccggcg agccgccggt cgtctccagg
      241 tcgagcgcca cgaacgtcac ctcggacagc gccgcaccga ggtcgtcgaa cgatgtctgg
      301 tgggggagca tccgccctcc tcgcggaggg ccgcgcggac gaggatgcta ccccgaacgt
      361 gcgttcgagc gcaagtgggt ggggcccggg cgcgggtcag gcgacgcggc gcatcaggcg
      421 gcggcgctcc tgctcggtgg tgccgcccca gacgccgtag cgctcgccgt tctggatggc
      481 ccactccagg cagatctcgc gcacgctgca ggcgttgcag taggagaggg ccaggccggc
      541 ctcctcctcg gaggtcggga agaagatctc cgggtcgagg ccgtagcagg cggcccgctc
      601 ctgccaggcg tcgggcgggg ggacctgctc gcgccgctcg tctcgagagg gtgcctcgtc
      661 catcgtctcc tcgggtcggt catcctaccc gacggtaggt atctcgccaa gggtcggatc
      721 gtcagtttcg tccgatttcc acgcggcagc gcgagaaccg gagccgcagt ctatcgctac
      781 actccgcccg gatggaagcc ccgatcccgc ggacccgctt ccggtgcggg agctgtggaa
      841 acctcacccg gttcgacgtg accgagcacc gccggacgcg cgcctactgg cacttcacga
      901 tcgagggcga gctcaccgtg gaggacgagg aggtgctcgc cgccgaccgc gaggaggtcc
      961 gctgccgctg gtgcggcgcg accgacggga tcgaggaagt cgcggtcgat ggctgaagcg
     1021 gccgcgacgg gcaacgggcc gctcgccggg atccgtgtcc tcgacctgac gcgtctcctc
     1081 ccgggcgggt actgcacgct gctgctcgcc gacctcgggg ccgacgtggt gaaggtcgag
     1141 gagccggggc gcggcgacta cgtccgctgg tcggcgccgg cggtcgacgg ccagagcgcc
     1201 gcccaccgcg ccctgaaccg cgggaagcgc tcgatcaccc tgaacctgaa gacccccgac
     1261 ggcgtggacc tgctcctgcg cctggtggag cggttcgacg tcctggtgga gtcctaccgg
     1321 ccgggcgtgc tcgaccgcct cggcgtcggg cacgcggccg tctcggcgcg caacgccggc
     1381 atcgtctact gcgcgatcag cggctacggg caggacgggc cgtaccgcga cctggcaggg
     1441 cacgacgtga actacctcgg ctacgccggc gtgctcggga tcaccggggg gcccgacggc
     1501 atgccggtgc tgcccggcgt gcagatcggc gacctgggcg gcggggccct gctcggcgcc
     1561 gccggcatcc tggcggccct cgtggaccgg gaacgcaccg gccgcgggcg cttcgtcgac
     1621 gtggcgatgc tcgacggcgt gttctcgtgg ctctcgatgc acctcgggaa cttcctggcc
     1681 gacggccggg tccccggccc gtcgcggatg ccgctgaacg gcggctacgc ctgctaccgg
     1741 gtctaccgcg ccggcgacgg ccggcacctg gcggtcggcg cgctcgagcc gcagttctgg
     1801 cgcgggctcg tcgaggcgct cggccggccg gacctcgccc aggagcagtt cgcgccgcgc
     1861 caggaggaga tcgcggccga gctgcaggcg atcttcgacg agaagccgcg ggatgagtgg
     1921 gtcgcggcgc tggggccgct ctcggtgtgc gtcggcccgg tgaacgactt cgccgaggcg
     1981 gtgcgcgatc cgcagatcat cgcgcggggc atggtggcac gggtcgacgg ctcgccggtc
     2041 ggcccgtcct cgccgctgcg gttctccgga acgggcgggc cggcgttgcg gccggcgccg
     2101 gagctggggg agcactcggc cgaactgctg agcgaggtcg gcgtggacga ggccgcgctc
     2161 gcggagctgc gcgcggcggg agcggtgtga ccgtacgtcg ttgcccggcc ccgcgggcgg
     2221 ggggacaatg cctcgcggaa cccgcaccga cgcgaccgcg accgacctcc cggtgagagc
     2281 gtgatacgga tctatctggt cggcacgacc agggacggca agaagctgct cttcgcgcgg
     2341 acgcgcgggg cgaagttcgg ctcgatgctc gtgccgatca cgccgcagct ggcgaagctc
     2401 gtcgccgggg cgcagcgttc accgctgcgc accctgacgc gcaccgagcc gcgcatcgtc
     2461 cccgtcgccc cgggcccggc gcgcccgctc cggcccgcgc cggcgccgga ggaaccggcc
     2521 ccggaggagc cggtcgcgga ggggtccgag ccgcgcacga tcccgatccc cgagcccggc
     2581 gaggtgatcc ggctccctgg cggcgcgcgg gtgatccgcg tctcccctgg cgagttccgc
     2641 cggctgacgg gcctcgcacc gcccgcgccc gaacccgaac ccgagcctga gcccgaaccg
     2701 ggaccggcgc ccgaagcgga gcccggggag ccggccgagc ggggagccga gggcgccgtc
     2761 cccgagacgc gccccacgcc ggaaccgaag gtgcgagcgg cgcggtccgg ggcgagggcc
     2821 aggccgaagg cgaggccgaa agcgcggccg aaggccaaac ggaaggccaa accgaaggcc
     2881 gagcccaagc ccgagccgaa gaccgagccg aagaccgagc cgaagaccga gccgccgccg
     2941 ccgaagcggc cggagatccg ctcgcggctg agcccggcgc agatccaggc cctcctgcgg
     3001 cgaggacgga gcgcccggtc ggtggctgcg ctcgccggcg cgccgctcga gtgggtgcag
     3061 cgctggctgg agccgatcct gtccgagcgc gtcggggtgg tcggcgagct gaagcgggcc
     3121 tggttctcga agccgcgcct ggggcgctcc gcggtcccga tcggcgtggc gatcgaggag
     3181 aacctgcgcg accgtggcgt ggccttcccg gagcgggtgg ccgccgacgg ctggcgtgcc
     3241 caccggccgg agggcggcac ctggcggctg cgcttcacct accaccaccg cggcgccgcg
     3301 cgctcggcac agtggtcgta cgacccggcg acgcgcaccg ccgcggcccg caaccggctg
     3361 gccgcggagc tctcctggcg gccgccgccg ggcgcgaccg cggaggccac tccgccgaag
     3421 caccgccgga gcaccggccg tccgcgggcg gtgcgccgga cggccacgcc ggcccgccgg
     3481 ca
//
LOCUS       DASPWO010000127         8627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_276684, whole genome shotgun sequence.
ACCESSION   DASPWO010000127 DASPWO010000000
VERSION     DASPWO010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276684"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..587)
                     /locus_tag="VEO00_02840"
     CDS             complement(18..587)
                     /locus_tag="VEO00_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56973.1"
                     /translation="MNGAPDRPTAGGGAGPDRGTVPLVTREERAASTEDRLSMLWERY
                     ADAQEEARRQAEANALLKAEIRVLREELARERAQLRAFVAAALAQQPVVLPDSEVVHP
                     AGREADDAPQEPLGLTLRERLAIREREREEREREERERRPALQAARLEAEARRREALA
                     RRREETYGSPATDDSWTAAPGPRFRRRRR"
     gene            705..962
                     /locus_tag="VEO00_02845"
     CDS             705..962
                     /locus_tag="VEO00_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56974.1"
                     /translation="MTAVLILLLIAAIFGVLGTVLKVALIIVLSVTLAVVLLGAMVWW
                     GVRRRLDRFLRQQRSSGDGPGRPIDVPGRREADPGLPPKAP"
     gene            complement(970..2589)
                     /locus_tag="VEO00_02850"
     CDS             complement(970..2589)
                     /locus_tag="VEO00_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HYU56975.1"
                     /translation="MRGLMQDDQLVLTRFLERAAAFHPHSPIVTRTSGGTERTTYGEL
                     AARAARLAGALRDLGIGRGDRVATFAWNSARHLELYFAVPLAGAVLHTLNVRLHHDQV
                     AWIVRHAEDRAFFVDGALARAWGPVAEALGPDAARVVELRDRDEAGAIPGSIGYEDLL
                     ASAEPPPAWPDLDEDDAALLCYTSGTTGPPKGVLSSHRSLVLHAQMVNQAAVLGLTER
                     DTVLPVVPMFHAAAWGLPYAATLAGARQVFASRWSGDPAAIAGLLESERVTVAAGVPT
                     VWIGLLRYLEDDPHDLSALRVIKTGGAAVPPALIERYETELGVPILQGWGMTETGPLA
                     SIASLPAELQSLPDPERYRLRAGQGRAVPGIRTRVVDPATGAEVPHDGTSAGELEARG
                     AWVASGYYRDEERSRDALDDGWLRTGDVATVDEHGLIHLVDRTKDLVKSGGEWISSLQ
                     LENALMGHPAVVEAAVVAVPDERWGERPLACVVIAPGAGVSPEDLRAFIEPRFPRWWL
                     PERFAFLDALPKTSVGKFDKRALRARFAAGP"
     gene            2681..3064
                     /locus_tag="VEO00_02855"
     CDS             2681..3064
                     /locus_tag="VEO00_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56976.1"
                     /translation="MANSRGMANPRGAANTTIEEQGELVWEGQLGSTFLRVDILPSGR
                     LAARWKSHGNDRRVIVDTETELERRVLFGLMVGADQAAQATMAQIMAAVSDAKVGRPD
                     PTQAPRRKKKRTRPSRRGMPRPRRR"
     gene            3090..3452
                     /locus_tag="VEO00_02860"
     CDS             3090..3452
                     /locus_tag="VEO00_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006941091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HYU56977.1"
                     /translation="MEVLDVVAQARDALSVKRVFGEPYERNGTTIIPAARIQGGAGGG
                     GGEGPPSGGGKGAGTGFGLSARPVGAYVIRGGEVSWQPAIDLNRIILGGQVVALVALL
                     TIRAVVKARAKAATRGGR"
     gene            3510..4082
                     /locus_tag="VEO00_02865"
     CDS             3510..4082
                     /locus_tag="VEO00_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016932608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HYU56978.1"
                     /translation="MERENVGGGDRGEGIGGYESLYGNTAAIGEAIAASLRERGMDVV
                     AGPISRIESTAATGTDVLVAGGPTHAHGMSRAGTRRTGASDEKNRFAEPTVEPGLREW
                     LDALLAAAGRTAAPFDTRLNKPRALTGSAAKGIAKRLERRGYRLVAEPESFFVTGKNE
                     LEDGEIDHAARWAATVADRVRADAGAPVSR"
     gene            complement(4072..4254)
                     /locus_tag="VEO00_02870"
     CDS             complement(4072..4254)
                     /locus_tag="VEO00_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56979.1"
                     /translation="MVLLDGEPTWGFLYRRMMPILARAHRVITTDLFGFGRSEHFLQE
                     DRGEAIAERITAWLSG"
     gene            complement(4355..4879)
                     /locus_tag="VEO00_02875"
     CDS             complement(4355..4879)
                     /locus_tag="VEO00_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYU56980.1"
                     /translation="MNLSAKAPADNPFPNKRIYSFGHRNMFGLAFDPQGGGLWISENG
                     PECNDEVNLIVAGGNYAWGQHETCAGTAPGNTNQDGPQPRLLPEVFYVSTIAPTGAEF
                     CNGCGLAGQSGHLLFGANNDGKIRSLTLDAGRDDVASQSVAYDHPSAVISMIAAPNGQ
                     IYFSDPSGIWRLTP"
     gene            complement(4876..5418)
                     /locus_tag="VEO00_02880"
     CDS             complement(4876..5418)
                     /locus_tag="VEO00_02880"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYU56981.1"
                     /translation="MRRFALIATILAVLGGVPAPAHAGIGATLVKGGLAFPTAFAFVP
                     NGTRIFYLERFSGEIRLLNLTNGNDTLFYTVPNIVSDGERGLLGITLHPDYPTRPYVY
                     VYATRSVTGAKNQLIRLTNSGRTGTGFKALASFTSVSGYHNGGAIRVGPDRRLYALVG
                     DGHNSANAQTLDNNLGKVCG"
     gene            complement(5458..6594)
                     /locus_tag="VEO00_02885"
     CDS             complement(5458..6594)
                     /locus_tag="VEO00_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYU56982.1"
                     /translation="MSTRHYTLPIEQTKWEVEGGCTTVFNWDYDDTRDRLLNLYEKGK
                     DKQWNASHRLDWSIEVDPTSPDNGPDAYIPIFGSDMWEALTDAEKGNLRRHMGAWLNS
                     QFLHGEQGALICAAKIVQTVPDVDSKFYASTQVIDEARHVEMYSKYLGQKIEMAYPIN
                     PNLKELLNQVIADTRWDMTYLGMQVIIEGLALAAFSLIRDFTDEPLAKAINTYVMQDE
                     ARHVAFGRLALRDYYPQLSDAERGEREEFVVEACYLMRDRFRAQEVWQHLGFDVERCM
                     EYVENSEMMREFQRMLFSRIVPTVKDIGLWGPKIRKAYEDMGVIQFSEVDVDALQAQD
                     EEIARKLDQSKGLSEEAEAATAIAGIDQARALEVQQTIEAGRPD"
     gene            6917..8206
                     /locus_tag="VEO00_02890"
     CDS             6917..8206
                     /locus_tag="VEO00_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU56983.1"
                     /translation="MASEAEPGMRGLWLGILVFRWATFAWMAIAAATRWHTFRRPGLA
                     LAAVLITGAWNAWFSLTWGWRRPRARWVDLAIAIALLPVSGLVMAEGAISRELFFATA
                     YPASAALTMGAGAGLAGLLAGAALSVGLVFSRLANGLAPGDLIADWANLVNGSVYYLA
                     AAAATGVVRRVLVTSAAERADALEEAARQRDRAVRLAERDALSREIHDSVLQALALVG
                     KKGKELANLPSVPPEELRELIDLTARQERALRTLLSEPPGEPPEGMMAVKAALDEAAS
                     GIRGVPVTVTSVGLTWMPTGTMSEVARAVHEALENVVEHAQATRATVFAEEVDRQLVI
                     SVRDDGVGFTYDEERLARDGKLGLLKSMKGRVEGLRGAMAVHTAEGRGTEVEFRLPME
                     EVTSGHGRERSRGGAERRADQGSRSRRPPRVARRDTV"
     gene            8109..>8627
                     /locus_tag="VEO00_02895"
     CDS             8109..>8627
                     /locus_tag="VEO00_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU56984.1"
                     /translation="MGESDRGEERSGEPIRVLVVDDHPVWRDGIRSDLEASGVAVVVA
                     EAADGGEAIELANEHMPDVVLMDLQLSTVSGVEATRRIAEDAPAIKIMVLSATGEEAD
                     VLEAVKAGAAGYILKSESGGDIVDAVRRVHAGEPVFTSGLAGLVLSEFRRVAGKEAGE
                     PGLTQRENEILRL"
BASE COUNT         1193 a         2979 c         3200 g         1255 t
ORIGIN      
        1 gcccgcgccc ggccgggcta gcgccgccgg cggcggaacc gcgggccggg ggctgctgtc
       61 caggagtcgt cggtcgccgg cgacccgtag gtctcctcgc ggcggcgggc cagcgcctcg
      121 cgacggcgcg cctcggcctc cagccgggcg gcctggagcg ccggccgtcg ctcgcgctcc
      181 tcgcgctcgc gctcctcgcg ctcccgctcg cggatcgcca gccgctctcg gagcgtgagc
      241 ccaagcggct cctgcggcgc gtcgtcggcc tccctgccgg cgggatggac gacctcggag
      301 tccggcaaca cgaccggctg ctgcgccagc gccgcggcga cgaaggcacg gagctgggcg
      361 cgctcgcggg cgagctcctc gcggagcacc cggatctccg ccttgaggag cgcgttggcc
      421 tcggcttggc gccgggcctc ctcctgcgcg tcggcgtagc gctcccacag catggagagg
      481 cggtcctccg tcgaggcggc gcgttcctcg cgcgtgacga gcgggaccgt tccccgatcg
      541 ggaccggccc cgccgccggc ggtgggcctg tccggggcgc cgttcatccg ttgagtgtgc
      601 gaacggggcc cgagatcgtc tatagcccaa acgggccatt tccggcgggg cgggacgacg
      661 aaacggaggg acgcgatccc accatcgccc gtaagatggg gtacgtgacg gccgtcctga
      721 tcctgctgct gatcgccgcg atcttcggcg tgctcggcac cgtgctgaag gtcgcgctga
      781 tcatcgtact ctcggtcacc ttggccgtgg tcctgctcgg ggcgatggtc tggtgggggg
      841 tgcgccgccg gctggaccgc ttcctgcgcc agcagcggtc gtcgggagac ggccccgggc
      901 gcccgatcga cgtccccggg cggcgagagg ccgaccccgg tctgcctccg aaggcgccct
      961 agccccgcgt cacgggccgg cggcgaagcg ggcccgcagc gcccgcttgt cgaacttccc
     1021 gacgctcgtc ttggggaggg cgtcgaggaa cgcgaatcgc tccggcagcc accaccgcgg
     1081 gaagcggggc tcgatgaagg cccggagatc ctcgggcgag acgccggcgc cgggcgcgat
     1141 caccacgcag gccagcggcc gctctcccca ccgttcgtcc gggaccgcca ccaccgcggc
     1201 ctccaccacg gcggggtggc ccatcagcgc gttctccagc tggagcgagg agatccactc
     1261 tccgcctgac ttcaccaggt ccttcgtccg gtcgaccagg tggatcaagc cgtgctcgtc
     1321 cacagtggcg acgtcgccgg tgcgcagcca gccgtcgtcg agcgcgtccc gcgagcgctc
     1381 ctcgtcgcgg tagtagccgg acgccaccca ggccccgcgc gcctcgagct cgccggccga
     1441 cgtcccgtcg tgagggacct cggcgccggt ggccggatcg accacccggg tgcggatccc
     1501 gggcacggcg cgcccctggc ccgcgcgcag ccgatagcgc tcggggtccg ggagcgactg
     1561 cagctcggcc ggcaggctgg cgatcgaggc cagcggcccg gtctcggtca tcccccagcc
     1621 ctgcaggatc gggacgccga gctcggtctc gtaccgctcg atcagcgccg ggggaaccgc
     1681 cgcgccaccg gtcttgatca cccgcagcgc agagaggtcg tgcgggtcgt cctcgaggta
     1741 acggagcagc ccgatccaca cggtcggcac gccggcggcg accgtgacgc gctccgactc
     1801 gagcaggccc gcgatggcgg ccgggtctcc cgaccatcgc gaggcgaaca cctggcgggc
     1861 gccggcgagc gtggcggcgt acggcagccc ccaggcggcg gcgtggaaca tcggcaccac
     1921 cgggaggacg gtgtcgcgct cggtgaggcc gagcacggcg gcctggttga ccatctgcgc
     1981 gtgcagcacc agcgagcggt gagaggagag gacgcccttc ggcggtccgg tggtcccgct
     2041 cgtgtagcag agcagcgccg cgtcgtcctc gtcgagatcc ggccacgcgg gcggcggctc
     2101 ggcggacgcc agcaggtcct cgtagccgat cgagccgggg atcgcgccgg cctcgtcccg
     2161 gtctcgcagc tcgaccacgc gagcggcgtc cgggccgagg gcctcggcca ccggacccca
     2221 ggccctcgcc agcgcgccgt cgacgaagaa cgcgcggtcc tccgcgtggc gcacgatcca
     2281 ggcgacctga tcgtggtgca ggcggacgtt cagcgtgtgc agcacggcgc cggcgagggg
     2341 gaccgcgaag tacagctcca ggtggcgggc ggagttccag gcgaacgtcg cgacgcgatc
     2401 accgcggccg atgcccaggt cccgcagcgc cccggccagt cgcgcggcgc gcgcggccag
     2461 ctcgccgtag gtcgtgcgct ccgtgccgcc ggaggtccgg gtgacgatcg gggagtgggg
     2521 atggaacgcg gccgcccgtt cgaggaagcg cgtcaggacc agctggtcgt cctgcatcag
     2581 tccgcgcacc ggccgatcgt agcaacgggg gtcgtgctac agtcgatgcg tactccaaca
     2641 ggcgcgccgt ttcatcgcgc gcgccgcgag aagggaccgc atggcgaatt ccaggggtat
     2701 ggcgaacccg cgcggggctg ccaacacgac catcgaggaa cagggcgagc tggtctggga
     2761 gggccagctg ggatccacgt tcctgcgggt ggacatcctc ccgtcgggcc gcctggccgc
     2821 gcgctggaag agccacggca acgaccgccg cgtcatcgtc gacaccgaga cggagctgga
     2881 gcggcgggtg ctgttcggcc tgatggtcgg cgccgaccag gccgcccagg ccacgatggc
     2941 ccagatcatg gccgcggtct ccgacgccaa ggtcggccgc cccgacccga cgcaggcgcc
     3001 acggcgcaag aagaagcgca cccggccctc gcgccgcggc atgccccgcc cccgccgccg
     3061 ctagctcgct ccggcgagag gaggcattga tggaggtact ggatgttgtc gcgcaggcgc
     3121 gcgacgcgct cagcgtgaag cgggtcttcg gcgagcccta cgaacggaac ggcacgacga
     3181 tcatccctgc cgcccggatc cagggcggcg cgggcggcgg cggcggcgag gggcctccgt
     3241 ccggcggggg gaagggtgcc ggcaccggct tcggtctgag cgcccgtccg gtcggggcgt
     3301 acgtgatccg cggcggcgag gtgtcgtggc agccggcgat cgacctcaac cggatcatcc
     3361 tcggcggcca ggtcgtcgcg ctcgtcgccc tgctgacgat ccgggccgtg gtgaaggccc
     3421 gggcgaaggc cgcgaccaga ggcggccgct gagaggcccc gcgtcgcggg gtcgtccggc
     3481 cctgccgccg ggaaggagcc tcctccagca tggagcgcga gaacgtcgga ggaggcgatc
     3541 gcggtgaagg cattggtggt tacgagtcgc tctacggcaa cacggcagcg atcggcgagg
     3601 cgatcgccgc gtcgcttcgc gaacggggga tggacgtggt ggccgggccg atctcccgga
     3661 tcgagtccac ggccgcgacc gggacggacg tgctggtcgc cggcggcccg acacatgcgc
     3721 acgggatgag ccgggccggg acgcggcgga cgggagcctc cgacgagaag aaccggttcg
     3781 cggagccgac cgtcgagccc gggctgcggg agtggcttga cgccctcctg gcggcggccg
     3841 ggcggacggc ggcgccgttc gacacgcggc tcaacaagcc ccgcgcgctg accgggtcgg
     3901 cggcgaaggg gatcgccaag cggctcgagc ggcgcgggta ccggctggtc gccgagccgg
     3961 agtccttctt cgtcaccggg aagaacgagc tggaggacgg ggagatcgac cacgcggcgc
     4021 gctgggcggc cacggtcgcc gaccgggtac gggcggacgc gggagcgccg gtcagccgct
     4081 gagccaggcc gtgatccgct cggcgatcgc ctccccgcgg tcctcctgca ggaagtgctc
     4141 ggaccggccg aacccgaaca ggtcggtggt gatgacacgg tgggcgcggg ccaggatcgg
     4201 catcatcctc cggtacagga agccccaggt cggctcgccg tccagcagca ccacggggtc
     4261 gccggaaccg tcgagctcga ggttgcgtgc ctcgaacggc cagcccggga ggtcctcgaa
     4321 cctcgcgtcg ggcggtggac cgcccccatc ccggctacgg cgtcagtcgc cagatgccgc
     4381 tcgggtcgct gaagtagatc tggccgttcg gcgctgcgat catcgagatc acggcactcg
     4441 ggtggtcgta cgcaaccgac tgggacgcca cgtcgtcgcg cccggcgtcg agcgtcagcg
     4501 agcggatctt gccgtcgttg ttcgccccga acagcaggtg gcccgactgc cccgccagcc
     4561 cacagccgtt gcagaactcg gccccggttg gggcgatggt tgagacgtag aacacctcgg
     4621 gcaacagccg cggctgcggg ccgtcctggt tcgtgttccc gggcgccgtg ccggcgcagg
     4681 tctcgtgctg gccccaggcg tagttcccgc cggccacgat gaggttcacc tcgtcgttgc
     4741 actccgggcc gttctccgag atccatagcc cgccgccctg cgggtcgaag gccagcccga
     4801 acatgttccg gtgcccgaag gagtagatcc gcttgttcgg gaacgggttg tcggccgggg
     4861 ccttcgcgga gaggttcatc cgcagacctt gcccaggttg ttgtccaggg tctgggcgtt
     4921 cgcggagttg tggccgtcgc cgaccagcgc gtacaagcgg cggtcggggc cgacccggat
     4981 cgcgcccccg ttgtggtacc cgctcaccga cgtgaaggag gcgagcgcct tgaatcccgt
     5041 gccggtcctc ccggagttcg tgagccggat cagctggttc ttcgctccgg tcacgctgcg
     5101 cgtggcgtac acgtagacgt acggcctggt cgggtagtcg gggtgcagcg tgatcccgag
     5161 cagcccgcgc tccccgtcgg agacgatgtt cgggacggtg tagaagaggg tgtcgttgcc
     5221 gttcgtcagg ttcaggaggc ggatctcgcc ggagaagcgc tcgaggtaga agatccgcgt
     5281 cccgttcggc acgaaggcga acgcggtcgg gaaggccagc ccacccttga ccagcgtcgc
     5341 gccgatgccg gcgtgcgccg gggccggcac gccccccagg acggccagga tcgtggcgat
     5401 cagggcgaac cggcgcatcg cgccacccta ccgaacgcaa gaccggagcg tggtcggtca
     5461 gtcggggcgg ccggcctcga tcgtctgctg cacctcgagc gcgcgggcct ggtcgatccc
     5521 ggcgatcgcc gtggcggcct cggcttcctc cgacagcccc ttgctctggt cgagctttcg
     5581 ggcgatctcc tcgtcctgcg cctgcagcgc gtcgacgtcc acctccgaga actggatcac
     5641 gcccatgtcc tcgtaggcct tacggatctt tggcccccac agcccgatgt ccttgaccgt
     5701 cggaacgatg cgggagaaca gcatcctctg gaactcgcgc atcatctcgg agttctccac
     5761 gtactccatg caccgctcga cgtcgaaccc gaggtgctgc cagacctcct gcgcccggaa
     5821 gcggtcacgc atcaggtagc aggcctccac gacgaactcc tcgcgctcgc cgcgctcggc
     5881 gtcggagagt tgcgggtagt agtcgcgcag cgccaggcgg ccgaacgcaa cgtggcgcgc
     5941 ctcgtcctgc atgacgtagg tgttgatcgc cttcgccagc ggctcgtcgg tgaagtcgcg
     6001 gatcagcgag aacgccgcca gtgccaggcc ctcgatgatc acctgcatcc ccaggtaggt
     6061 catgtcccag cgcgtgtcgg cgatgacctg gttcagcagc tccttcaggt tcgggttgat
     6121 cgggtaggcc atctcgatct tctggccgag atacttcgag tacatctcga catggcgcgc
     6181 ctcgtcgatg acctgcgtcg aggcgtagaa cttcgagtcg acgtcgggga ccgtctgcac
     6241 gatcttcgcc gcgcagatca gcgcgccctg ctcgccgtgc aggaactggg agttgagcca
     6301 ggcccccatg tgccgccgca ggttgccctt ctccgcgtcg gtcagggcct cccacatgtc
     6361 cgagccgaag atcgggatgt acgcgtcggg gccgttgtcc ggcgacgtcg gatcgacctc
     6421 gatcgaccag tcgagacggt ggctggcgtt ccactgcttg tccttgccct tctcgtacaa
     6481 gttgagcagc cggtcgcgcg tgtcgtcgta gtcccagttg aagacggtcg tgcagccgcc
     6541 ctcgacctcc cacttcgtct gctcgatcgg cagcgtgtag tgacgcgtgc tcatcggctc
     6601 cctcctcggc tcggtacggg cgcgtgcgcg cgggcgccgt ccccgcgggc ggcggggacc
     6661 atgacctggg cgatctccca atcgacggac gtacgcgtcg cgctctcgcc gagcgtcccg
     6721 gcgtctccca tgaccaaagg ctatctcgcc cgccgctacc ggatgcgagc catgcaaggg
     6781 ttcgaccgcg aaggatctgt tcgggcagat tcgttcgggt tcatccgacg cgccaaggcc
     6841 ctccgggctg ttgaccgtta gggacgtgcg tgccacgctc gcacggggag gccacatggc
     6901 gcgaacgaga ggcccggtgg catccgaggc tgagcccggc atgcgcgggt tgtggctcgg
     6961 gatcctcgtc ttccgctggg cgacgttcgc gtggatggcg atcgccgccg cgacacggtg
     7021 gcacaccttc cgtcgaccag gcctggcgct cgcggcggtg ctgatcacgg gcgcgtggaa
     7081 cgcgtggttc agcctgacgt ggggatggcg ccgaccgcgt gcccgctggg tcgacctcgc
     7141 gatcgcgatc gccctgctcc cggtgtccgg cctggtgatg gccgagggag cgatcagtcg
     7201 agagctcttc ttcgccaccg cctatcccgc gtccgccgcg ctgacgatgg gagcgggggc
     7261 cgggctggcc ggactgctcg ccggcgccgc gttgtcggtg gggctcgtct tcagccggct
     7321 ggcgaacggc ctcgctcccg gagacctgat cgccgactgg gcgaacctgg tgaacggttc
     7381 cgtctactac ctggcggccg cggccgcgac gggcgtggtt cggcgggtcc tggtgacctc
     7441 ggccgccgag cgcgcggatg cgctcgagga ggccgccagg cagcgcgacc gggcggtccg
     7501 gctggccgag cgagatgccc tcagccggga gatccacgac tcggtgctgc aggcgctggc
     7561 gctcgtcggg aagaagggca aggagctcgc gaaccttccc tccgttccac cggaggagtt
     7621 gcgtgagctc atcgacctca cggcgcgaca ggagcgtgcg cttcgaacct tgctcagcga
     7681 gccacccggg gagcctcccg agggcatgat ggcggtcaag gccgcactcg atgaggccgc
     7741 ctcggggatc cggggggtcc cggtgaccgt cacctcggtc ggcctcacct ggatgccgac
     7801 cggcacgatg agcgaggtgg cccgcgccgt tcatgaagcc ctggagaacg tggtcgagca
     7861 cgcccaggcc acgcgcgcca ccgtcttcgc cgaggaggtc gatcgccagc tcgtgatatc
     7921 ggtccgcgac gacggggtcg gtttcaccta cgacgaggag cgccttgccc gtgacgggaa
     7981 gcttgggctg ctgaagagca tgaagggtcg ggtcgagggc ctccgagggg cgatggccgt
     8041 gcacacggcc gagggtcggg gcacggaggt ggagttccgc ctgccaatgg aggaggtgac
     8101 gagcggtcat gggcgagagc gatcgcgggg aggagcggag cggcgagccg atcagggttc
     8161 tcgtagtcga cgaccacccc gtgtggcgcg acgggatacg gtctgacctg gaggcgtcgg
     8221 gagtcgccgt ggtcgtcgca gaggcggcgg acggcggtga ggcgatcgag cttgcgaacg
     8281 agcacatgcc cgacgtggtc ctgatggatc ttcagctgtc gaccgtgtcg ggggtggagg
     8341 cgacccgccg gatcgccgag gacgcgccgg cgatcaagat catggtcctg tcggcgaccg
     8401 gtgaggaggc cgacgtgctc gaggccgtca aggcgggtgc ggccggctac atcctgaaga
     8461 gcgagtccgg gggcgacatc gtcgacgcgg ttcggcgggt ccacgccggc gagccggtgt
     8521 tcacgtcggg actggccggt ttggtgctct cggagttccg gcgcgtcgcc gggaaggagg
     8581 cgggagagcc ggggctcacc cagcgggaga acgagatcct gcgcctc
//
LOCUS       DASPWO010000128         3667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_21279, whole genome shotgun sequence.
ACCESSION   DASPWO010000128 DASPWO010000000
VERSION     DASPWO010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21279"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1037)
                     /gene="jag"
                     /locus_tag="VEO00_02900"
     CDS             complement(171..1037)
                     /gene="jag"
                     /locus_tag="VEO00_02900"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HYU56985.1"
                     /translation="MEWVETTGRTVEEALDAALDELGVDEDEVEFEVLEAARGGLLAR
                     REARIRARVRPLSREKPGEQRRRRARRGAPTRPERTAARPDGGAVAVDDRPAGDAGRS
                     GGPSRRRRRSRGGRGARAEATGAAMTEDGVSEPSVSVEEQAEVAEGFARGLVEAFGAD
                     ADVVARIDEDAVLVDITGGDLGLLVGPRGVTLQAIEDLVRTVVQRQTEGHGTRINVDV
                     GGYRAKRREALAAFAHELADQVRESGREQALEPMSAADRKVVHDTVAELDGVGTASEG
                     EEPRRRVVIRPA"
     gene            complement(1048..2157)
                     /gene="yidC"
                     /locus_tag="VEO00_02905"
     CDS             complement(1048..2157)
                     /gene="yidC"
                     /locus_tag="VEO00_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HYU56986.1"
                     /translation="MLDPIYEAIGWLLSAFYSVVPNLGIAIILLTFTIMLILYPLTAK
                     QAKSMIAMQRLQPEIKRLQAKYKNDRQKLNEEVMKVYQENKVNPLAGCLPLLVQLPIF
                     FSLFHVLRDSYKYVPLNSNLFADLCRHDGVVAKTVDACVKGNLDVNHLKFLSMDLQKA
                     ATDVSGGVFTALPYFILVLLVMVTGFMQTRQAQRRTPQTNKQMAMVMKVLPVFFGVIS
                     LQFPAGLVLYFFVSNLWRLGQQEVIFRRHGTAALPAGGKGAIDVASKERPAKAPPREE
                     LEEAPGEAPAEAPTERPAPRTAPDRPAAAPKAVPTARRSGGGLRGLFQVPPPEANGGT
                     ATGAAPPAVRKAATGGAPPKPPPPRKRRNKKKRKR"
     gene            complement(2170..2481)
                     /gene="yidD"
                     /locus_tag="VEO00_02910"
     CDS             complement(2170..2481)
                     /gene="yidD"
                     /locus_tag="VEO00_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HYU56987.1"
                     /translation="MRRARRRPTIPARALQMPVRAWRLVSVHLPPRCRFEPSCSEYAL
                     GALDRHGAARGSWLALRRLARCHPWHPGGFDPVPPPRAEAVSTNTRSPYRVPEHTAET
                     C"
     gene            complement(2478..2807)
                     /locus_tag="VEO00_02915"
     CDS             complement(2478..2807)
                     /locus_tag="VEO00_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HYU56988.1"
                     /translation="MIGRVRDRATFEALARAGRVQRGAITLRYVASHGDGVPRAAFAI
                     GRGTGGAVRRNRARRRLRAALLDVADELEPGGAYLVGAGAGVVTMPYDALRDQLRAVV
                     RAAAETR"
     gene            complement(2804..2938)
                     /gene="rpmH"
                     /locus_tag="VEO00_02920"
     CDS             complement(2804..2938)
                     /gene="rpmH"
                     /locus_tag="VEO00_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HYU56989.1"
                     /translation="MKRTFQPNNRKRKKTHGFRVRMRTRGGRAVIRNRRSRGRKRLAA
                     "
     gene            3225..>3667
                     /locus_tag="VEO00_02925"
     CDS             3225..>3667
                     /locus_tag="VEO00_02925"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HYU56990.1"
                     /translation="MPVDNRDRPEPAADDIAQRIWEQASTSLRAELAEATWRTWFQGV
                     RAVRFEHDVLVLTAPSTVAADRIRSSYQAMLEDAARVATGRDLAVELLVDVDTRARTP
                     AAAEAPPPEPHAMTALASPPAAPTVSSSAPSELPWAAATLNPRYT"
BASE COUNT          477 a         1401 c         1261 g          528 t
ORIGIN      
        1 cgcccgagcc gaggtccagg aacggcccat ccggctcccc gacgatccgg gcgaaggcct
       61 cggcgtgccg cagctgctcc tccgcggggc cgggacccaa gaagccccgg cgttgcgctt
      121 cggcgagagc gtcgagcagc cgatcccgca cggctgcgcc gctcgtgcgc ttaggccggg
      181 cggatgacca cgcgtcggcg gggctcctcc ccctccgacg ccgtgcccac accgtcgagc
      241 tccgccaccg tgtcgtgcac gaccttccga tcggctgccg acatcggctc cagcgcctgc
      301 tcgcgcccgc tctcccgcac ttggtcggcg agctcatggg cgaaagccgc cagggcctcc
      361 cgccgcttgg cccggtagcc gccgacatcg acgttgatcc gggtcccgtg cccctcggtc
      421 tggcgctgca ccaccgtgcg aacgaggtcc tcgatcgcct ggagcgtcac gcctcgtggc
      481 cccaccagga gccccaggtc gcccccggtg atgtccacga gcacggcgtc ctcgtcgatc
      541 cgggcgacca cgtcggcatc ggcgccgaac gcctcgacca ggccccgggc gaagccttcc
      601 gccacttcgg cctgctcctc caccgacacg ctcggctcgc tcacgccgtc ctccgtcatc
      661 gctgcccctg tcgcctcggc ccgggcgccc ctgccgcccc ggctgcgccg gcgacgccgg
      721 ctcggcccgc cggatcgccc ggcgtcgccc gccggccggt cgtccaccgc cacggccccg
      781 ccgtcgggtc tggccgcggt gcgttcgggt cgtgtcggag cgccgcggcg cgcccgccga
      841 cgccgctgct cgccgggctt ctcccgggag agcggccgca cccgagcccg gatccgcgcc
      901 tcccgacggg ccagcaaccc gccccgggcg gcctcgagca cctcgaactc gacctcgtcc
      961 tcgtccacgc cgagctcgtc gagggcggcg tccagcgcct cctcgacggt ccggccggtg
     1021 gtctcgaccc actccacggg ccgggcctca gcgcttccgc ttcttcttgt tgcggcgctt
     1081 ccgcggcggc ggcggcttgg gcggtgcacc gcccgtggcc gcctttctca ccgccggcgg
     1141 tgccgcgccg gtggcggtgc cgccgttcgc ctccggcggc ggcacctgga acaagccgcg
     1201 gaggcctcct ccgctgcgcc ttgccgtcgg caccgccttc ggcgcggcgg cgggccggtc
     1261 gggagcggtg cgtggtgccg gccgctccgt cggggcttcg gccggtgcct cgcccggtgc
     1321 ctcctcgagc tcctcccgag gtggcgcctt cgccgggcgc tccttgctgg cgacgtcgat
     1381 cgcgcccttc ccgccggcgg gaagagcggc ggtgccgtgg cgccggaaga tgacctcctg
     1441 ctggcccagc cgccagaggt tgctgacgaa gaagtacagc accaggccgg cggggaactg
     1501 cagcgagatg accccgaaga agacgggcag aaccttcatc accatggcca tctgcttgtt
     1561 ggtctgcggc gtccgccgct gggcctgccg ggtctgcatg aagcccgtca ccatcaccag
     1621 cagaacgagg atgaaatacg ggagcgcggt gaagacgcct ccgctcacat ccgtcgccgc
     1681 cttctggagg tccatcgaca ggaacttcag gtggttgacg tcgaggttcc ccttgacgca
     1741 cgcatcgacg gtcttcgcga cgactccgtc gtgacggcac aggtcagcga agagattcga
     1801 gttcagcggg acgtacttgt acgagtcgcg caacacatgg aagagcgaga agaagatcgg
     1861 gagctggacc aggaggggga gacaaccggc gagcgggttc accttgttct cctggtagac
     1921 cttcatcacc tcttcgttga gcttctggcg gtcgttcttg tacttcgcct gtagccgctt
     1981 gatctccggc tggagccgct gcatggcgat catcgacttc gcttgcttcg cggtcagcgg
     2041 atagaggatc aacatgatgg tgaaggtcag caggatgatc gcgatcccga ggttcgggac
     2101 cacggagtag aaggccgaca gcagccagcc gatggcttcg tagatcgggt cgagcatgcg
     2161 tggcctctct cagcaggtct cggccgtgtg ctccggcaca cggtagggcg aacgtgtgtt
     2221 cgtgctcacc gcttcggcgc gtggcggcgg aacggggtcg aagccgcctg ggtgccaagg
     2281 atggcacctc gcgagccggc gcagtgcgag ccagctcccg cgcgccgcgc cgtgtcgatc
     2341 gagtgcgccc agcgcgtact ccgagcacga cggctcgaag cggcatcgcg gcggcaggtg
     2401 cacgctgacc agtcgccacg cgcgcaccgg catctgcaac gcgcgcgcag ggatcgtcgg
     2461 gcgccggcgc gcgcgtctca tcgcgtctcc gccgcggcgc gtaccaccgc gcgcagctgg
     2521 tcgcggagtg cgtcgtacgg catcgtcacc acgcctgctc ccgcgcccac gaggtacgcg
     2581 ccgcccggct cgagctcatc ggccacgtcg aggagcgcgg cgcggagccg tcgacgggcc
     2641 cggttgcgtc gtaccgcgcc gccggtgccg cgcccgatcg cgaacgcggc gcgcggaacg
     2701 ccatcgccgt gcgatgcgac gtagcgaagt gtgatcgcgc cgcgttgcac gcggccggcg
     2761 cgggcaagag cctcgaacgt cgcacggtcg cggacgcgcc ctatcaggcg gcgagccgct
     2821 tccgcccccg gctgcggcgg tttcggatca cggcccgccc accgcgcgtg cgcatgcgga
     2881 cgcgaaaccc gtgcgtcttc ttgcgcttgc ggttgttcgg ctggaacgtg cgcttcaacg
     2941 gggaggcctc ctggcctgcg cgggccgagg cgggggccgc gcagagagtc gctggagtct
     3001 acgggcacgg gcccccgctc gcaacgcgcg cgcgcacacc cactcttgtc gcaccagata
     3061 cccggtgcta cgttccgccg tcgcccgccg atccggcctc gacgcggcga cgacttcccc
     3121 gccggggacc tgccccggac cggccggcca gtcccttcgg ggacgcagag ccatgccaac
     3181 gggggaggcg gccgccgccc ccacagttct ccacaactgt ggacgtgcct gtggacaacc
     3241 gggatcggcc cgagccggcc gccgacgaca tcgcccaacg aatctgggag caagcgtcga
     3301 cctcgttacg ggccgagctc gccgaggcca cctggcgcac gtggttccaa ggtgtccggg
     3361 ccgtgagatt cgagcacgac gtgctcgtgc tgaccgcgcc gagcaccgtc gccgccgacc
     3421 ggatccggtc cagctaccag gccatgctcg aggacgccgc ccgggtcgcg accggccggg
     3481 acctggcggt cgagctgctc gtcgacgtcg acacccgggc ccggacgccg gccgccgccg
     3541 aggctccgcc gcctgagccg cacgccatga cggcgctcgc cagcccgccg gcggcgccca
     3601 cggtgtcctc gagcgcaccg agcgaactcc cgtgggccgc ggcgaccctc aacccccgct
     3661 acacctt
//
LOCUS       DASPWO010000129         6326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1042207, whole genome shotgun sequence.
ACCESSION   DASPWO010000129 DASPWO010000000
VERSION     DASPWO010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042207"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /gene="nusA"
                     /locus_tag="VEO00_02930"
     CDS             complement(<1..1143)
                     /gene="nusA"
                     /locus_tag="VEO00_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HYU56991.1"
                     /translation="MDLQNLMGALRELERDKGVPLETILSGLEEALAAAYKSWRRGFG
                     DEPDDARAEMDPETGNIRMFVQTLEEEPEQAEGAERPERPERAERKVIEEHEVDLPAD
                     FIGRIAAQTAKQVIYQKLREAEHEQTYEEFSGREGDIVTGIIQQDSHRYTLLDLGKVE
                     ALLPQAEQVPSEPYRHGERLKAYIVEVRRSAKGPQIVVSRTHPGLLKKLFELEVPEIA
                     EGIVEIKAVAREPGHRSKIAVASNEPGVDPVGACVGAKWARVRMVVNELRGEKIDVVP
                     WYEDARQYVAAALQPAKVKEVRIDPDTQTALVIVPDYQLSLAIGKEGQNARLAARLTG
                     WRIDIKSEAQIQEDAAKAAARPEPEPSPEPEPEEAPTPEPEPSPEPE"
     gene            complement(1151..1615)
                     /locus_tag="VEO00_02935"
     CDS             complement(1151..1615)
                     /locus_tag="VEO00_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HYU56992.1"
                     /translation="MELENLVRPVVEAGGLELVEVGFAREGGRRVLRVTVDREGGVDL
                     DAIAAMSERLSRRLDAADAVAGRYSLEVSSPGIERRLRTPRDFARSVGRKVVVKTALP
                     ESGARTVTGTLEAADEEAATLATEQGPVRVRYADVVSARTMFEWPGKAPAKR"
     gene            complement(1900..2178)
                     /locus_tag="VEO00_02940"
     CDS             complement(1900..2178)
                     /locus_tag="VEO00_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56993.1"
                     /translation="MAAKIPREVREFAKYHADAEAVIQRIGRNLFDLVVVDPDGNWVR
                     AVFPAEEDARDAADRLGVRAHDGWNERMSARMNRRDHWGEPGGQRRAL"
     gene            complement(2190..3065)
                     /locus_tag="VEO00_02945"
     CDS             complement(2190..3065)
                     /locus_tag="VEO00_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HYU56994.1"
                     /translation="MAKAGKLGGWRKIASAMWGPPDDPQVYGILEFDATNVLAYQARA
                     RAAGHHVTATHFVGRALAVALHDVPDFNVRIVKGKVIPRETVDIFFITAVEGGRELSG
                     VKIERTDEKPVTEVADELSDRATRMKAGDDPEFAKTKRSIAGMPRRLLRPSMRLSAWI
                     AGDHNRRIKSLGVKASPFGSAMVTSIGMFGISQGFAPLARFYRMPLLVLVGEITDKPM
                     AVEGRVEVRPVLPLTATIDHRYADGWHISQLLRPFKAYLGDPSGFEPDPTPPDEPVEP
                     PKPALAELPAEGPAP"
     gene            3150..4571
                     /gene="proS"
                     /locus_tag="VEO00_02950"
     CDS             3150..4571
                     /gene="proS"
                     /locus_tag="VEO00_02950"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HYU56995.1"
                     /translation="MALPRQAEDFPGWYQEVVKQADLAENSLVRGTMVIKPYGYAIWE
                     AIQRAVDDRIKATGHQNLYFPMFIPYRLLEKEAEHVEGFAPEIAVVTHAGGEELGEPL
                     VVRPTSETIIWDAYARWVQSYRDLPLLYNQWANVVRWELRPRLFLRTTEFLWQEGHTA
                     HETAEEAMAETRLALLEIYRDAVESVLAMPVHAGRKSASQRFPGAVETYTIEALMRDR
                     KALQAGTSHYLGQNFARAYGVRFLGRDGEEHYPYATSWGVSTRLVGGVIMAHGDDRGL
                     RLPPLLAPVQAVVVPIFRTDDERGRVLEACARIAADLSAFRMKVDDRDDLRPGFKFND
                     WELRGVPLRVELGPRDLEHDRATLVSRLSGEKEEVALDGLPARVEVLLEEIQAGLYEE
                     ALAFREAHTFRPVDFAEMVAVLADPGGFVITGWCGDEACEDKVKEETKATIRVLPMEP
                     GAEGSCIVCGKVAAEEATWALAY"
     gene            complement(4585..5271)
                     /locus_tag="VEO00_02955"
     CDS             complement(4585..5271)
                     /locus_tag="VEO00_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56996.1"
                     /translation="MEEARWDRIAAVSGLLFVALLMLTVFMVPRPPDPGTPADAVRTF
                     FVDNDNAIRISVFVAGLAGIAFLWFLGSLRAYLRRAEPQGGRLSAVAFGAGIAALAAV
                     APSYGLQATAASTATGGGGAGLVLLLYDLGFALQTLAAFAGAALVGAASISAFRSGAL
                     PRWLAGVGALLAVAETVSGADLFLDSTVLGPQGAYGFTVFFLFLLWVVVVSAVMIARL
                     GGGARGPKAS"
     gene            complement(5284..6129)
                     /locus_tag="VEO00_02960"
     CDS             complement(5284..6129)
                     /locus_tag="VEO00_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HYU56997.1"
                     /translation="MIVEERAGKLRQAPAPLDRLRFGTYLAPSVLPVYALVTEEVGHR
                     LGIETELVVETTYDSCERDENEVCFVCSLPYVIFERRGLDLAVPVAAPVLLGARYGGR
                     PIYFSDVIVHRDSPFRSFLDLRGRSWAYNEPLSHSGYGITRYRLVTLGETDGFFGDVI
                     EAGFHQEAVRMVARGEVDGSAIDSQVLAIEVRDHPDLAERVRVVEALGPSTIQPVAVS
                     RRVPEDLREAIRDVLVTMAEDPALRARLADGLVERFVPVDARAYDDIRAMVDACEAAR
                     FIELR"
     gene            complement(6126..>6326)
                     /locus_tag="VEO00_02965"
     CDS             complement(6126..>6326)
                     /locus_tag="VEO00_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU56998.1"
                     /translation="LVGALLAAFFIPSRDPQRPVTGRVTPLRLGVAIGGAALSLALLA
                     IEARGLSGSPCAAVRESPSSGA"
BASE COUNT          906 a         2324 c         2219 g          877 t
ORIGIN      
        1 ctcgggctcg ggcgacggct cgggctcggg cgtgggagcc tcctccggct cgggctcggg
       61 cgacggctcg ggctctgggc gggccgccgc cttcgccgcg tcctcctgaa tctgggcctc
      121 gctcttgatg tcgatccgcc agccggtcag ccgggcggcg agacgggcgt tctgcccctc
      181 cttgccgatg gcgagcgaca gctggtaatc gggcacgatg accagcgcgg tctgggtgtc
      241 cgggtcgatc cggacctcct tgaccttcgc cggctgcaac gccgccgcga cgtactgccg
      301 cgcgtcctcg taccagggca ccacatcgat cttctcgccg cgcagctcgt tgaccaccat
      361 ccgcacgcga gcccacttcg cgccgacgca ggcgccgacc gggtcgacgc ccggctcgtt
      421 ggaggccacc gcgatcttcg agcggtgccc cggctcgcgg gccaccgcct tgatctcgac
      481 gatcccctcg gcgatctcgg ggacctccag ctcgaacagc ttcttcagca ggccggggtg
      541 ggtgcgcgag acgacgatct gcgggccctt cgccgaccgg cggacctcga cgatataggc
      601 cttcaggcgc tcgccgtggc ggtagggctc cgacggcacc tgctccgcct gcggcagcag
      661 cgcctcgacc ttgcccaggt cgagcagcgt gtagcggtgc gagtcctgct ggatgatccc
      721 ggtgacgatg tcgccctcgc gcccggagaa ctcctcgtag gtctgctcgt gctcggcctc
      781 gcgcagcttc tggtagatga cctgcttggc cgtctgggcc gcgatccgcc cgatgaagtc
      841 ggcgggcagg tcgacctcgt gctcctcgat gaccttccgc tcggcgcgct cggggcgttc
      901 ggggcgctcg gcgccctcgg cctgctcggg ctcctcctcc agggtctgga cgaacatgcg
      961 gatgttgccg gtctccgggt ccatctccgc ccgcgcgtcg tcgggctcgt cgccgaaccc
     1021 gcgccgccac gacttgtagg cggctgccag cgcctcctcg agcccggaca ggatcgtctc
     1081 gagcggaacg cctttgtccc gctccagctc ccgcagggcg cccatcaggt tctgcagatc
     1141 caccgcaccc tcacctcttc gccggggcct tccccggcca ctcgaacatc gtccgcgccg
     1201 acaccacgtc ggcgtagcgc acccgcaccg gcccttgctc ggtggcgagc gtcgccgcct
     1261 cctcgtcggc ggcctcgagc gtcccggtga cggttcgggc gccactctcc ggcagcgccg
     1321 tcttcaccac caccttccgt ccgacgctgc gggcgaagtc gcgcggcgtg cgcagccgcc
     1381 gctcgatgcc gggcgaggag acctccagcg agtagcggcc ggccaccgcg tcggccgcgt
     1441 cgagcctccg ggacaggcgc tcggacatcg ccgcgatcgc gtcgaggtcc acacctccct
     1501 cccggtcgac cgtcacccgc agcacgcgcc gcccgccttc ccgggcgaag ccgacctcca
     1561 cgagctcgag cccgccggcc tcgacgaccg ggcggaccag gttctccagc tccaccgcgc
     1621 ccctcgcaaa agaacgtggg cctgccgccc acgtccgggt aaaaccgctg ctcaaacggc
     1681 agtatagcac gtgaatacgt gaaaatcggc cgccggaggc gggcgacgac agcaggtcgc
     1741 cgctcgtcag ccggaagcgg aggaacaagt tcctcgccgg acggcgaacg cggccggctg
     1801 ctcccgcgag aggtgttcgg tagcaggcgg tggttcgcac gccggacgcg gcccggcttg
     1861 cgctccccag gggcgagcta tctcccgaac cgccccgccc tacagggcgc ggcgctggcc
     1921 gcccggctcg ccccagtggt cgcggcggtt catccgggcc gacatccgct cgttccagcc
     1981 gtcgtgggca cggacgccga ggcggtcggc cgcgtcgcgg gcatcctcct cggcggggaa
     2041 caccgctcgg acccagttgc cgtcggggtc gaccaccacg aggtcgaaca ggttccgccc
     2101 gatccgctgg atcacggcct cggcgtccgc gtggtacttg gcgaactccc ggacctcgcg
     2161 ggggatcttc gcggccacgg ggccggagcc tagggcgccg gtccttcggc gggcaactcc
     2221 gccagcgcgg gcttcggcgg ctcgaccggc tcgtcgggcg gcgtcgggtc cggctcgaag
     2281 ccggacgggt cgccgaggta ggccttgaac ggccgcagca gctggctgat gtgccacccg
     2341 tcggcgtagc ggtggtcgat cgtcgccgtc agggggagga ccggccgcac ctccacccgc
     2401 ccctcgacgg ccatcggctt gtcggtgatc tctccgacca ggacgaggag cggcatgcgg
     2461 tagaagcgcg ccagcggtgc gaacccctgc gagatgccga acatcccgat gctcgtgacc
     2521 atggcgctcc cgaacgggct cgctttcacg ccgagcgact tgatccggcg gttgtggtcg
     2581 ccggcgatcc aggcggagag gcgcatcgag gggcgcagca ggcgtcgtgg catcccggcg
     2641 atgctcctct tcgtcttggc gaactcgggg tcgtcccccg ccttcatccg ggtggcgcgg
     2701 tccgagagct cgtcggccac ctccgtcacg ggcttctcgt cggtccgctc gatcttgacc
     2761 cccgacagct cccggccgcc ctcgacggcc gtgatgaaga agatgtcgac ggtctcgcga
     2821 gggatgacct tgcctttcac gatgcggacg ttgaagtcgg ggacgtcgtg cagcgccacg
     2881 gccaatgccc gtccgacgaa gtgggtggcg gtgacgtggt ggccggccgc gcgggcgcgg
     2941 gcctggtacg ccagcacgtt ggtggcgtcg aactccagga tgccgtagac ctgcgggtcg
     3001 tccggcggcc cccacatcgc cgaggcgatc ttgcgccacc cgccgagctt cccggccttc
     3061 gccatcgccc tagcctgcca gcagcccgag cgccgcacaa ggggtggttc gcggccggga
     3121 gcgggaccgc ctccgctatc ctgcctgtca tggcgcttcc gaggcaggcg gaggacttcc
     3181 cgggctggta ccaggaggtc gtcaagcagg ccgacctcgc ggagaactcc ctggtgcgcg
     3241 ggacgatggt gatcaagccc tacggctacg ccatctggga ggcgatccag cgcgccgtgg
     3301 acgaccggat caaggccacc gggcaccaga acctctactt cccgatgttc atcccgtacc
     3361 ggctcctgga gaaggaggcc gagcacgtcg agggcttcgc gccggagatc gcggtggtca
     3421 cccacgccgg cggcgaggag ctcggcgagc cgctcgtcgt ccgcccgacc tccgagacga
     3481 tcatctggga cgcctacgcg cgctgggtgc agtcgtaccg ggacctgccg ctgctgtaca
     3541 accagtgggc gaacgtggtg cgctgggagc tgcggccgcg gctgttcctg cggacgacgg
     3601 agttcctgtg gcaggaaggc cacacggcac acgagacggc cgaggaggcg atggccgaga
     3661 cgcgcctggc cctgctggag atctaccgcg acgccgtcga gtcggtgctg gcgatgccgg
     3721 tccacgccgg gcgcaagagc gcctcccagc gattcccggg cgccgtggag acgtacacga
     3781 tcgaggcgct gatgcgcgac cgcaaggcgc tgcaggccgg cacgtcccac tacctggggc
     3841 agaacttcgc ccgggcgtac ggggtgaggt tcctggggcg cgacggggag gagcactacc
     3901 cctacgccac gtcctggggg gtcagtacgc ggctggtcgg cggggtgatc atggcccacg
     3961 gcgacgaccg cgggctgcgc ctcccgccgc ttctggcgcc cgtccaggcg gtcgtcgtcc
     4021 cgatcttccg caccgacgac gagcgcggtc gcgtcctcga ggcctgcgcc cggatcgctg
     4081 cagacctttc cgccttccgg atgaaggtgg acgaccgcga cgacctccgg cccgggttca
     4141 agttcaacga ctgggagctg cgcggggtgc cgctgcgcgt ggagctcggg ccgcgcgatc
     4201 tggagcacga ccgggccacg ctcgtgtcaa gactgagcgg ggagaaggag gaggtcgccc
     4261 tcgacgggct gccggcccgg gtcgaggtgc tgctggagga gatccaggcc gggctgtacg
     4321 aggaggccct ggcgttccgc gaggcgcaca ccttccggcc cgtggacttc gcggagatgg
     4381 tcgccgtgct ggccgacccc ggcgggttcg tgatcaccgg gtggtgcggt gacgaggcct
     4441 gcgaggacaa ggtcaaggag gagacgaagg ccacgatccg ggtcctgccg atggagcccg
     4501 gggccgaggg ctcctgcatc gtctgcggca aggtggcggc cgaggaggcc acctgggcgc
     4561 tggcctactg acggctggcg ctcgttagct ggccttcggc ccccgcgcgc cgccaccgag
     4621 gcgggcgatc atcaccgcgc tgaccacaac cacccacagc aggaacagga agaacaccgt
     4681 gaacccgtag gccccctggg ggccgaggac cgtggagtcc aggaacaggt cggctcccga
     4741 caccgtctcg gcgaccgcga ggagggcccc gaccccggcc agccaccgcg gcaacgcccc
     4801 cgaacggaag gcggagatcg aggcggcccc caccagggcg gcccccgcga aggccgccag
     4861 ggtttggagc gcgaagccaa ggtcgtacag gagcagtacc aaaccggcgc cgcctccgcc
     4921 ggtcgcggtg gaggccgcgg tggcctgcag accgtacgac ggtgcgacgg cggccagcgc
     4981 ggcgatgccg gcgccgaagg ccaccgccga cagccggcca ccctgcggct ccgctctccg
     5041 gaggtacgcg cgcaggctcc ccaggaacca gaggaaggcg ataccggcca gcccggccac
     5101 gaagacggag atgcggatcg cgttgtcgtt gtcgacgaag aacgtccgga ccgcgtccgc
     5161 cggggtgccc gggtccggcg gccgcggaac catgaacacc gtcagcatca gcagggccac
     5221 gaacagcagt ccgctcaccg ccgcgatccg atcccaacga gcctcttcca tgcccctccc
     5281 tctctagcgg agctcgatga accgggcggc ctcgcaggcg tcgaccatgg cccggatgtc
     5341 gtcgtaggcg cgcgcgtcca cgggcacgaa ccgctcgacc agcccgtccg ccagccgggc
     5401 gcgcagcgcg gggtcctcgg ccatcgtcac cagcacgtca cggatcgcct cccgcaggtc
     5461 ctccggtacg cgccgggaca ccgccaccgg ctggatcgtc gacgggccga gcgcctcgac
     5521 cacccgcacc cgctcggcga ggtccgggtg gtcacggacc tcgatggcca gcacctgcga
     5581 gtcgatcgcc gacccgtcca cctcgccccg ggcgaccatg cgcaccgcct cctggtggaa
     5641 ccccgcctcg atcacgtccc cgaagaagcc gtccgtctcc cccagggtca ccaggcggta
     5701 gcgggtgatc ccgtaccccg agtgcgagag cggctcgttg tacgcccagg agcgcccgcg
     5761 gaggtcgagg aacgaccgga acgggctgtc gcgatggacg atcacgtcgg agaagtagat
     5821 cggccggccg ccgtagcgcg cgcccaggag gaccggcgcg gccaccggaa cggccaggtc
     5881 caggccgcgc cgctcgaata tcacgtacgg caggctgcag acgaagcaga cctcgttctc
     5941 gtcccgctcg cagctgtcgt aggtggtctc gacgacgagt tcggtctcga tgcccagccg
     6001 gtgcccaacc tcctcggtca ccagcgcgta gaccggcagc acgctcggcg ccaggtaggt
     6061 cccgaaccgc agccgatcca acggcgctgg cgcctgccgc agcttcccgg cccgctcctc
     6121 gacgatcacg ccccggagga tgggctctcc cgaacggccg cgcaagggga gccgctcagg
     6181 cctcgcgcct cgatcgccag caacgcgagt gagagagccg ccccgccgat cgccactccc
     6241 aggcgaagtg gggtcacgcg gccggtcacc ggacgttgtg ggtcccgcga ggggatgaag
     6301 aacgccgcga ggagcgcgcc gaccag
//
LOCUS       DASPWO010000130        11941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_362157, whole genome shotgun sequence.
ACCESSION   DASPWO010000130 DASPWO010000000
VERSION     DASPWO010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11941
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362157"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VEO00_02970"
     CDS             <1..210
                     /locus_tag="VEO00_02970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016908069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU56999.1"
                     /translation="EAGMAKYFCTEACHEIVTESLRVHGGYGYSTEYTIERLYRDAPF
                     LLIGEGTSEIQKLVISRQLLERYRA"
     gene            596..949
                     /locus_tag="VEO00_02975"
     CDS             596..949
                     /locus_tag="VEO00_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57000.1"
                     /translation="MARLTFTLEDEVARELERAERHLFRSRAKLLAAIDQLPVEQAFL
                     LGPVVGWLEQLDEQPSQAAIVRQAVAMYLNAIREVERSAALDEGYAALAADEEREAII
                     EAAARRVWDQWGDEP"
     gene            931..1272
                     /locus_tag="VEO00_02980"
     CDS             931..1272
                     /locus_tag="VEO00_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HYU57001.1"
                     /translation="MGRRAVGTPAFRGQIWLADAGVGEAKRFVIVSNNQRNERLADVL
                     GARLTTAPKPSIPSVAEFAAGEISESASRVVADDIVPIKKRFLIRQDGALTSGQMRRV
                     EDALRNALALG"
     gene            1304..1543
                     /locus_tag="VEO00_02985"
     CDS             1304..1543
                     /locus_tag="VEO00_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HYU57002.1"
                     /translation="MVVRVDQELLDRVDRLISGGSVGSRSKAIRVGLGQLIEGGQRAE
                     LGRRIVDGYVRFSPADDPDGWAEAAARAMIEAEPW"
     gene            complement(1569..2489)
                     /locus_tag="VEO00_02990"
     CDS             complement(1569..2489)
                     /locus_tag="VEO00_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HYU57003.1"
                     /translation="MVALCVALAACTAPPADERRAPPDPVVPSAPAPSPTVTSASPSP
                     RTAAAEPLPTPDEPIPKGPAEIAAALTDTTQRLHAAIDRWRHEGDPARPGIPEDVELL
                     ALYQQRITRGLAREADRWRRVRPLLPGWLRGEVIANVRAAAELLAIITPIEGDAPFET
                     APPPPAGELLRSFKEGERRFGVDWEVLAAVCYIESKFARVKAASTAGAQGPMQFLPST
                     WEVYGMGGDINDPHDAILGAANYLHASGAPADDAAALFAYNHSDAYVDAILRHAGQIR
                     DDPNDYFAYYAWQVFVVTTDGDRRLTGPGL"
     gene            2542..2892
                     /locus_tag="VEO00_02995"
     CDS             2542..2892
                     /locus_tag="VEO00_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HYU57004.1"
                     /translation="MVALIVRIVQWNLNGTPTSIPELRRYLRDESVDAFSEVPGLRFK
                     VWIADEATNRWGAVYLWESREAAAGPLPSRAAQMIGKVPDCAEWWEVEATVEGRFGDP
                     RLARLGRAFEDVVT"
     gene            2889..3323
                     /locus_tag="VEO00_03000"
     CDS             2889..3323
                     /locus_tag="VEO00_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU57005.1"
                     /translation="MTVPARLSIVTLGVDDLQRSIAFYEALGWRKSSASIEGVIAWFH
                     TNETALGLFPRAELAADAALPSEPRAPFGGITLAMNIEHEELVQPLLEAAAAAGGSIL
                     KPATRADWGGVSGYFADPDGHPWEIAWNPGFELDEDGRLRLP"
     gene            3427..3927
                     /locus_tag="VEO00_03005"
     CDS             3427..3927
                     /locus_tag="VEO00_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020461554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HYU57006.1"
                     /translation="MGEARPFGRYLEDFEVGDVYRHWPGKTITEYDDHLFCAITWNHH
                     PLHTNAHYAETATQFGKNVVVGNLVYSLALGMSVPDVSGRAIANLEVESLQHKAPFFH
                     GDTLYAETTVLEVKESSSKPDRGIVTVETRGLNQRGEEVLYFRRKVMVPKRGTIEEQP
                     DRPEPR"
     gene            complement(3938..4837)
                     /locus_tag="VEO00_03010"
     CDS             complement(3938..4837)
                     /locus_tag="VEO00_03010"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HYU57007.1"
                     /translation="MTYSIVARDPDTGELGVAVQSRWFSVGSVVTWAEPGVGAVATQS
                     FAEVAHGPNGLRLLREGRTAGEALIELVAADEGEALRQVGIVDAAGNVAGHTGARCVA
                     DAGDVQDDEVSCQANMMASPSVWPAMLEAYRSTTGDLADRMLAALVAGEKAGGDARGR
                     QSAALVVVSGDREAPPWQRAIELRVEDHPDPVGELARLLRLQRAFTHLDRGQELADAG
                     RMEEGGAEAALAVALAPEDDQVAFFGALVLIGAGRVEEARPLLDRARAANPGWPAFLR
                     RFAASGTIPDDPALLDAIMPLEG"
     gene            4879..5571
                     /locus_tag="VEO00_03015"
     CDS             4879..5571
                     /locus_tag="VEO00_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HYU57008.1"
                     /translation="MAGRTAAGERGLAALTAEIVRCRKCPRLAAWRESVAADPPKRFA
                     AERYWARPVPGFGDAGARVLIVGLAPAAHGGNRTGRIFTGDRSGDWLFAALYRTGFAN
                     QPTSTGRDDGLRLHDAYVAAAVRCAPPGNRPAPEERDRCLPYLVRELALLERVRAIVA
                     LGAFAWDAALRTLGALGHRTKPRPSFAHAAEATVGPYVLLGSYHPSQQNTFTGVLTEE
                     MLDAVLTQAKML"
     gene            complement(5573..5728)
                     /locus_tag="VEO00_03020"
     CDS             complement(5573..5728)
                     /locus_tag="VEO00_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57009.1"
                     /translation="MCSDCGCGMPQENHGDDRHILWSQIVAAAEASGVSPQEAVNNMQ
                     EMAKKQS"
     gene            5854..6753
                     /locus_tag="VEO00_03025"
                     /pseudo
     CDS             5854..6753
                     /locus_tag="VEO00_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            6854..7873
                     /locus_tag="VEO00_03030"
     CDS             6854..7873
                     /locus_tag="VEO00_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU57010.1"
                     /translation="MALFDRIFEYLDMPHEITDAPDARDLSPHEVRGRVRFRDVWFRY
                     DRPAPDATLERIEAAAPAGADDPGVLARAVADVTADGEGREWALSGLDFEVQPGQLAA
                     LVGASGAGKTTVTYLIPRFYDVERGSVEIDDVPVERIKLASLGEIIGMVTQETYLFNT
                     TVRRNLLYGNPDATEEQVVEAARAGFIWDRIEEMPDGLETVVGERGYRMSGGEKQRLA
                     IARVVLKDPRILILDEATSALDTLSERLVQEALDPLMTGRTTIAIAHRLSTILAADVI
                     FVLDRGRIIERGTHAELLARGGSYARLYEEQFKGGEVEAVTEDGVVLASGEIIPAGPW
                     NTSAS"
     gene            complement(7836..8336)
                     /locus_tag="VEO00_03035"
     CDS             complement(7836..8336)
                     /locus_tag="VEO00_03035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU57011.1"
                     /translation="MSVEIHPVRPEEHAEAGAVVVAAYMEFFEPGHEGWERYLVHLGD
                     VAGRAERSIVLVAVEDGRMLGCATVELDEPISGGESDLGPDEARLRMLGVLPETRGRG
                     IGEALTRAAIEAARERGRTRMVLNSAEYMTQAHRIYERLGFSREPDWVGEDGFRMRTY
                     SMDLPG"
     gene            8419..8838
                     /locus_tag="VEO00_03040"
     CDS             8419..8838
                     /locus_tag="VEO00_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYU57012.1"
                     /translation="MAQDDLGRLLIEAYRTFDEEARAALDEQGLSDLRPSFVTVHAHI
                     DAEGTRVSDLARRAGLSKQAMTWLIEEMEGQTLLRRVDDPDDGRARRVLLTAKGRRRL
                     GQARRVVDRVEARVRRRLGERRHDALRAVLGDLRGAE"
     gene            complement(8854..9633)
                     /locus_tag="VEO00_03045"
     CDS             complement(8854..9633)
                     /locus_tag="VEO00_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HYU57013.1"
                     /translation="MSQSFLGRIMEAGRSPRMHPHVGPTDNADVERIAGTRSGALRAA
                     IFGANDGLVSNLSLIMGVAGANIGRTAILVAGVAGLIAGAFSMGAGEYVSMRVQRELF
                     ERLLHIEAHELATQPVEERRELARLYRLKGMPKEVAEQAADALMRDPQVALETHAREE
                     LGLDPNALGSPIGAALSSFVMFAIGAIAPLVPFLVAEGTAAILASVTIGAVALFALGA
                     GMARFTGRPVALSGGRMLLIGGTAAAVTYGVGSLLNVGGIT"
     gene            complement(9630..10544)
                     /locus_tag="VEO00_03050"
     CDS             complement(9630..10544)
                     /locus_tag="VEO00_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HYU57014.1"
                     /translation="MIFTPGERQAVWEAWGVDAVTFESRPTLREPALICAFAGWNDGG
                     ESASGAARFLREAWGATRFATIDPEEFFDFQVNRPTVRLIDGVTRHIEWPRNDFFHAS
                     AGTRDVVLFTGIEPNNKWRGFCEAIVAVGREVDADTLVTLGGFLADMPHTRAIRVAGS
                     APTQEEAEALGVAASRYEGPTGIVGVLQDVANRAGLRALSLWAAVPHYLPAGPNPKAA
                     LALVERLGRLLEVPIDTAPLSALATTWEERVDATVAENDELSEYVRRLEAGADQAEED
                     ELEIPSGDELARELERFLRRQGGGEGER"
     gene            10603..11076
                     /locus_tag="VEO00_03055"
     CDS             10603..11076
                     /locus_tag="VEO00_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HYU57015.1"
                     /translation="MSEEAGRRPRVTQKPPRPRDDLGRPLPHHAPNALELEDFDALSW
                     EENHRLGVEHFNAGRFFPAHEAWETAWKQLKGTPDEEFFKGLSQLGAGYVHYLRGNSH
                     GAFTLLRRAAGRIARFPAGHHGVDTDRLVAAALATADRVEEYRTVDPDDLDPPRI"
     gene            11312..>11941
                     /locus_tag="VEO00_03060"
     CDS             11312..>11941
                     /locus_tag="VEO00_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HYU57016.1"
                     /translation="MNVVVLTKYVPNPEGVPEMGDDFLLKREGVEGALDPGDEFGVEA
                     GLQLAEASGGEVTVVSMGPEPAMGGVRKALSMGAAKGVLVTDPKLRGSDVLVTARVLA
                     AAVKRLEPDIVVAGVESTDGYTGTLPMALAEFLGWPNVTFARHLEAGDGSVKVERQTE
                     TGYDVVESPLPAVVTVTAGANAPRYPTLKGIMASKQKPVEQLAAGDLGLS"
BASE COUNT         1730 a         4229 c         4254 g         1728 t
ORIGIN      
        1 gaggccggca tggccaagta cttctgcacc gaggcctgcc acgagatcgt gaccgagtcc
       61 ctccgcgtcc acggcggcta cggctactca acggagtaca cgatcgagcg cctctaccgc
      121 gacgccccct tcctcctgat cggcgaaggc accagcgaga tccagaagct cgtgatcagc
      181 cgccagttgt tggagcgcta cagggcctga gcacggcggg atgagcgggc cgtcggggac
      241 ccggcgtttc gaccgccgat ccttcagcca cctgttgtac cgatatagct cgatttggta
      301 tttccggctg cccagcagag cctcgtcgtg gaagcctcgc gtggctgacg cgacagcatc
      361 tctgaagtgg gcattgagga ccggtgaccg tcgcctccgg tacgcccacg gatcccttga
      421 tggtcaaagt cacccagatg aggatctggt tttcttccgg gaggtcggtc gccacggcgg
      481 aagcatgtgc gtgcgtcacg cccggaagca atgccgggtc acttggggga agcccgggtg
      541 ggcccgcgaa gttgcagcgg aatctgcagc acgatatgat gcatcgagtg atgcgatggc
      601 caggttgacg ttcacgctgg aggacgaggt cgcccgggag ctcgagcggg ccgagcggca
      661 cctgttccgt tcgcgggcga agctgctcgc ggcgatcgac cagcttccgg tcgagcaggc
      721 gttcctgctg gggccggtcg tggggtggct cgagcagctc gacgaacagc cgtcgcaggc
      781 ggcgatcgtg cgccaggcgg tggcgatgta cctcaacgcg atccgggagg tcgagcggtc
      841 ggccgcgctc gacgagggct atgccgcact tgccgccgac gaggaacgtg aggccatcat
      901 cgaggcggcg gcgcggaggg tctgggacca gtggggcgac gagccgtagg tacacccgcg
      961 tttcgaggcc agatctggct cgctgacgcc ggcgtcggcg aggcgaagcg gttcgtcatc
     1021 gtttccaaca accagcgaaa cgagcgtctc gctgacgtcc tcggggctcg tctgaccacg
     1081 gcgccaaagc cttcgattcc atcagtcgcg gagttcgcgg ccggagagat cagcgagtcg
     1141 gcaagccggg tcgtcgccga tgacatcgtc ccgatcaaga agcgattcct gattcgtcag
     1201 gacggcgcct tgaccagcgg ccagatgcga cgcgtcgagg acgcgctcag gaacgcgttg
     1261 gcgctcgggt gagccagccc gatcccgaac gaccatgaga tgggtggttg tacgcgtcga
     1321 ccaggagctg cttgatcggg tcgatcggct gatctcggga gggagcgtcg gttcgcgttc
     1381 gaaggcgatc cgggtcggct tgggacagct catcgaaggt ggccagcggg cagagctcgg
     1441 acgtcgaatc gtcgacggct acgtgcgatt ctcgcccgcc gacgatcccg acggctgggc
     1501 cgaggcggcc gctcgggcga tgatcgaagc ggagccctgg tgagagccgg gaatcgtggt
     1561 gagcccgctt agaggccggg gccggtgagg cgcctgtcgc cgtcggtcgt cacgacgaag
     1621 acctgccagg cgtagtaggc gaagtagtcg ttcgggtcgt cgcggatctg gccggcgtgg
     1681 cgcaggatcg cgtcgacgta ggcgtcggag tggttgtagg cgaacagggc cgcggcgtcg
     1741 tcggccgggg cgccggaggc gtgcaggtag ttggccgcgc cgaggatggc gtcgtgcgga
     1801 tcgttgatgt cgccgcccat cccgtagacc tcccacgtcg acggcaggaa ctgcatcggg
     1861 ccctgcgcgc cggcggtgct ggcggccttc acgcgggcga acttcgactc gatgtagcag
     1921 accgccgcca gcacctccca gtcgaccccg aaccgccgct cgccctcctt gaaggagcgc
     1981 agcagctcgc cggccggcgg cggcggcgcc gtctcgaacg gcgcgtctcc ctcgatcggc
     2041 gtgatgatcg cgaggagctc tgccgcggcc ctgacgttcg cgatgacctc gccgcggagc
     2101 cagccaggca gcagcggacg cacgcgccgc cagcgatcgg cttcccgggc cagcccgcgg
     2161 gtgatccgct gctgatagag ggcgagcagc tcgacgtcct cggggatgcc cggccgtgcc
     2221 gggtcgccct cgtggcgcca gcggtcgatc gctgcgtgca gccgttgcgt cgtgtcggtg
     2281 agcgccgccg cgatctccgc gggacccttg gggatcggct cgtcgggcgt ggggagcggc
     2341 tcggcggcgg ccgtacgagg cgagggcgag gccgaggtga cggtcgggct cggcgccggc
     2401 gccgacggca cgaccgggtc cggtggagca cgcctctcgt cggccggcgg agcggtgcac
     2461 gccgcgaggg cgacgcagag cgccaccatc agggccgaag ttgctcgccg ggcccaaggc
     2521 ccggatgctg ggagacggga ggtggtcgcg ttgatcgttc gcatcgtcca gtggaacctc
     2581 aacggcacgc ccacgagtat ccccgagttg cgccgctatc tccgcgacga gtcggtcgac
     2641 gcgttctcgg aggtccccgg cctgcgcttc aaggtgtgga tcgccgacga ggccacgaac
     2701 cgctggggcg ccgtctacct ctgggagtct cgcgaggccg ccgcgggccc gctccccagc
     2761 cgcgcagccc agatgatcgg gaaggttccg gactgcgccg agtggtggga ggtcgaggcg
     2821 acggtcgagg gacgcttcgg agacccgcgg ctcgcccgtc tcggcagggc gttcgaggac
     2881 gtggtcacgt gaccgtgccg gcgcgcctgt cgatcgtcac gcttggcgtc gacgacctgc
     2941 agcgatcgat cgccttctac gaggcactcg ggtggcggaa gtcgtcggcg tccatcgagg
     3001 gcgtcatcgc ctggttccac accaacgaga ccgcgctcgg gctcttcccg cgggcggagc
     3061 ttgccgcgga cgcggcgctc ccgtccgagc cgcgcgcccc gttcggcggc atcacgctgg
     3121 cgatgaacat cgagcacgaa gagctcgtcc agccgctgct ggaggcggcc gccgcggccg
     3181 gcggttcgat cctgaaaccg gcgacgcgcg cggactgggg aggcgtgtcc ggctacttcg
     3241 ccgacccgga cggccacccg tgggagatcg cctggaatcc cggcttcgag ctcgacgagg
     3301 acggccggct gcggctgccc tgagcagttc gacgacctcg ccgaacccgg ggatcgatcg
     3361 ttaccggatc gagttgcgct ctcccgcatc cggcggtgag catcccgggg acgaaggagg
     3421 aacgggatgg gagaggcgcg accgttcgga cggtatctcg aggacttcga ggtcggcgac
     3481 gtctacaggc actggccggg gaagacgatc accgagtacg acgaccacct gttctgcgcg
     3541 atcacctgga accaccaccc gctgcacacc aacgcgcact acgcggagac cgcaacgcaa
     3601 ttcgggaaga acgtggtggt cgggaacctc gtctactcgc tggcgctcgg tatgagcgtg
     3661 cccgacgtgt ccggtcgcgc gatcgccaac ctggaggttg agtcgctgca gcacaaggcg
     3721 cctttcttcc acggcgacac gctgtacgcg gaaaccaccg tgctcgaggt gaaggagtcg
     3781 tcgtcgaagc cggaccgcgg gatcgtcacc gtcgagacca gaggactcaa ccagcgcggc
     3841 gaggaggtcc tctacttccg gcgcaaggtg atggtcccga agcggggaac gatcgaggag
     3901 cagcccgacc ggcccgagcc gcggtagggc ccgctcctca accctcgagc ggcatgatcg
     3961 cgtcgaggag cgccgggtcg tcggggatcg ttccggatgc ggcgaaccgc cgcaggaagg
     4021 cgggccagcc cgggttcgcg gcgcgggcgc gatcgaggag cggccgcgcc tcctccaccc
     4081 ggcccgcgcc gatcaggacg agcgccccga agaacgccac ctgatcgtcc tccggcgcca
     4141 gggcgacggc aagcgccgcc tcggcgccgc cctcctccat ccggccggcg tccgccagct
     4201 cctggccccg gtccaggtgc gtgaacgccc gctgcagccg gagcaggcgt gcaagctcgc
     4261 cgacggggtc ggggtggtcc tcgacgcgga gctcgatcgc gcgctgccac ggcggcgcct
     4321 cgcgatcgcc gctcaccacg acgagcgccg cggactgccg cccgcgcgcg tcgccgcccg
     4381 ccttctcgcc ggcgaccagg gcggccagca tccggtctgc caggtcgccg gtcgtgctcc
     4441 ggtaggcctc gagcatcgcc ggccacacgc tcgggctggc catcatgttc gcctggcacg
     4501 acacctcgtc atcctggacg tcgccggcgt cggcgacgca gcgcgcgccg gtatgcccgg
     4561 ccacgtttcc ggccgcatcg acgatcccca cttggcgcag cgcctcgccc tcgtcggcgg
     4621 cgaccagctc gatgagggcc tcgccggccg tccgcccctc ccggagcagg cgtagcccgt
     4681 tcggtccgtg ggcgacctcg gcgaatgact gggtggccac cgcgccgacg ccgggctcgg
     4741 cccaggtgac caccgacccc accgagaacc agcggctctg cacggccacc cccagctcgc
     4801 cggtgtccgg gtcccgcgcc acgatcgagt acgtcacgcc gcctcctcca ccctggtcac
     4861 tcgtagcatg aggcctcgat ggccgggcgg acagcggcgg gggagcgcgg gctggcggcg
     4921 ctcaccgcgg agatcgtgcg gtgtcggaaa tgcccgcggc tggccgcctg gcgcgaatcg
     4981 gtcgcggccg acccgccgaa gcggttcgcg gccgagcggt actgggcccg gccggtaccc
     5041 gggttcggcg atgccggcgc tcgcgtgctg atcgtcggcc tggcgcccgc ggcccacgga
     5101 gggaaccgca ccggccgaat cttcaccgga gaccgctccg gcgactggct gttcgcggcg
     5161 ctctaccgga ccggattcgc caaccagccg acctcgaccg gtcgtgacga cggcctgcgg
     5221 ctgcacgacg cgtacgtggc ggcggccgtg cgatgcgcac cgcccgggaa ccgtcccgcc
     5281 cccgaggagc gcgaccgctg cctcccgtac ctggtccgcg agctggccct cctcgagcgc
     5341 gtccgggcga tcgtcgcgct cggcgcgttc gcctgggacg ccgccctgcg cacgctcggg
     5401 gctctcggcc atcgcacgaa gccccgcccg tccttcgccc acgccgccga ggcaacggtc
     5461 ggcccctacg tcctgctcgg ctcctaccat cccagccagc agaacacctt caccggcgtc
     5521 ctgacagagg agatgctcga cgccgtcctg acccaggcga agatgctgtg agctagctct
     5581 gcttcttcgc catctcctgc atgttgttca cggcctcctg gggcgagacg ccgcttgcct
     5641 cggcggcggc gacgatctgg ctccagagga tgtgccggtc gtcaccgtgg ttctcctggg
     5701 gcatgccgca cccgcaatcg ctacacacgc tgattccccc cttcctagaa ccaaacttcg
     5761 ctattttatt tccgcggtcg tggctgcgca aaccgaggcc agcctacccg cgcgccggcc
     5821 ggtgaaaccg ggcaccggtc gtatcctgag cccatgccac ggcgggggtt cggccactgg
     5881 gggtacggca atctcgacga ggacgccggt cccgtcaacc gccgcactct gcgccgggtg
     5941 gtccacgcgt tcgcgcccta tcggcgccag gtcgtctgga tcgggctcgc catcctggtc
     6001 acgtcgggcc tcggggtcgt caacccgctg ctgatcaagg tcgtcttcga cacggccctg
     6061 ttcacgccga ccggcgtggt gaacttccac cggctctacc tgttcgtcgg gttgatgatc
     6121 gccatcccgg tggtctctgg cgtgatcggc atcggccaga cctacatgtc gaacctcgtc
     6181 ggccagcacg tgatgcagga cttccgcaac gcgctgtacc gccacctgca acacatgccc
     6241 ctgcgcttct tcacggccac gcgcaccggc gagattcagt cgcgcatctc gaacgacgtc
     6301 gggggcgtgc agtcggtgat caccgacacc gcgtcgagcg tgctggcgaa cttcgtcatc
     6361 ctgctgtcaa ccctgatcgc gatgttcatc ctgtcctggc agctcaccgc gctgagcctg
     6421 gccctcatgc cgttcttcat ctggctcacc ggccgcgtcg gcaaggcgcg ccaggaagtg
     6481 gcgcgcaaca cgcagaagag cctggccgag atgacggcga tcaccgagga gaccctctcg
     6541 gtgtcgggga tcctgctgtc gaaggcgttc ggccggcagc gctacgagat caagcgcttc
     6601 gagacggaga acgagaagct cgccggcctg cagatccgcc agcagatgat cggccggtcg
     6661 ttcttcgcga cgatccagat cttcttctcg atcaccccgg cgctcgtcta cctggtcgcc
     6721 ggcctggtga tcgggaacga ccccgggcag acggcgatca cagccggcac gatcgtcgcg
     6781 ttcaccacac tgcagagccg gctgttcttc ccgatcgggc agatgctgca ggtgacggtc
     6841 gaggtgcagc gccgtggcgc tgttcgaccg gatcttcgag tacctcgaca tgccgcacga
     6901 gatcaccgac gcgcccgacg cgcgcgacct ctcgccgcac gaggttcgcg gaagggtgcg
     6961 cttccgcgac gtctggttcc gctacgaccg gccagctccg gacgcgacgc tcgagcggat
     7021 cgaggcggcg gccccggccg gcgccgacga tcccggtgtg ctggcgcggg cggtggccga
     7081 tgtgaccgcg gacggggagg gccgggagtg ggccctgtcg ggcctcgact tcgaggtcca
     7141 gcccgggcag ctggccgcgc tcgtcggcgc gagcggcgcc ggaaagacca ccgtgacgta
     7201 cctgatcccg cggttctacg acgtggagcg cggctccgtg gagatcgacg acgtgccggt
     7261 cgagcggatc aagctcgcct cgctcgggga gatcatcggg atggtcaccc aggagacgta
     7321 cctgttcaac acgaccgtcc gccgcaacct gctctacggc aaccccgacg ccaccgagga
     7381 gcaggtggtc gaggcggcga gggcggggtt catctgggac cggatcgagg agatgccgga
     7441 tgggctggag accgtggtcg gggagcgcgg ctaccggatg tccggcggcg agaagcagcg
     7501 cctggcgatc gcgcgcgtgg tgctgaagga tccccgcatc ctgatcctgg acgaggcgac
     7561 gagcgcgctc gacacgctct ccgagcgcct ggtgcaggag gcgctcgacc cgctgatgac
     7621 cggacggacg acgatcgcca tcgctcaccg cctgtcgacg atcctcgccg ccgacgtgat
     7681 cttcgtcctc gaccgcgggc ggatcatcga gcgcgggacg cacgccgagc tgctggcccg
     7741 gggcggctcg tacgcgcgcc tctacgagga gcagttcaag ggcggggagg tcgaggccgt
     7801 gaccgaggac ggcgtcgtgc tcgcgtcggg cgagatcatc ccggcaggtc catggaatac
     7861 gtccgcatcc tgaacccgtc ctcgccgacc cagtcgggct cgcgactgaa ccccagccgc
     7921 tcgtagatgc ggtgcgcctg cgtcatgtac tcggcgctgt tcaggaccat ccgggtgcgg
     7981 ccgcgctcgc gggcggcctc gatcgcggcc cgggtcagcg cctccccgat cccgcgcccg
     8041 cgggtctccg ggagcacccc gagcatgcgc aagcgggcct cgtcggggcc gaggtccgac
     8101 tcgccgccgc tgatcggctc gtcgagctcc acggtcgcgc acccgagcat cctgccgtct
     8161 tccacggcga cgagcacgat cgacctctcc gcccgcccgg cgacgtcgcc gaggtggacc
     8221 aggtaacgct cccagccctc gtgcccgggc tcgaagaact ccatgtaggc ggccaccacc
     8281 acggcgcccg cctcggcgtg ctcctcgggt cgtacgggat ggatctccac gctcacgccg
     8341 gccaggatac ggccgccgcg cggagaggcg atggtcaagg aacttgacca atccggccgg
     8401 ccgcggcagg atgggcgcgt ggcgcaggac gacctcggcc ggctgctgat cgaggcctat
     8461 cggaccttcg acgaggaggc ccgggcggcg ctcgacgagc agggcctgtc cgacctgcga
     8521 ccctccttcg tcaccgtgca cgcccacatc gacgccgaag ggacgcgggt gtccgacctc
     8581 gcccgccgcg ccgggctctc gaagcaggcc atgacctggc tgatcgagga gatggagggc
     8641 cagaccctgc tgcgccgcgt ggacgacccc gacgacggcc gtgcccggcg ggtcctcctg
     8701 accgcgaagg ggcggcgccg cctgggacag gcgcgccggg tggtggatcg ggtcgaggcc
     8761 cgggtccggc gccgtctggg cgagcgtcgc cacgacgccc tgcgcgccgt gctgggcgat
     8821 ctgcgcgggg ccgagtgacg caggatcgct ccgtcaggtg atgccgccga cgttgagcag
     8881 gctgccgacg ccgtaggtca ccgcggcggc agtcccgccg atcaggagca tccgcccgcc
     8941 ggagagcgcc acgggtcgtc cggtgaagcg ggccatcccc gcaccgagcg cgaagagcgc
     9001 gacggctccg atggtgaccg aggcgaggat cgccgccgtg ccctcggcga cgaggaacgg
     9061 caccagcggt gcgatcgcgc cgatggcgaa catcacgaac gacgagagcg ccgcgccgat
     9121 cggagatccg agcgcgttcg gatccaggcc cagctcctcg cgggcgtggg tctcgagcgc
     9181 cacctgcggg tcccgcatca gggcgtcggc ggcctgctcg gcgacctcct tcggcatccc
     9241 cttcagccgg tagagccggg ccagctcgcg gcgctcctcc accggctgcg tcgccagctc
     9301 gtgtgcctcg atgtggagca gacgctcgaa cagctcccgc tgcacgcgca tcgacacgta
     9361 ctcgccggcg cccatcgaga aggccccggc gatcaagccg gcaacgccgg ccaccaggat
     9421 cgcggtgcgg ccgatgttgg cgcccgcgac gcccatgatc agcgagaggt tcgagaccag
     9481 gccgtcgttc gccccgaaga tcgccgcgcg gagcgccccg ctgcgggttc cggcgatccg
     9541 ctcgacgtcg gcgttgtccg tcggtccgac gtgcgggtgc atccgcggtg accggcccgc
     9601 ctccatgatc cggcccagga acgactggct caccgctccc cctccccgcc gccctgtcgg
     9661 cgcaggaagc gctccagctc gcgggccagc tcgtcgccgc tcgggatctc cagctcgtcc
     9721 tcctcggcct ggtcggcgcc ggcctccagg cgccggacat actccgagag ctcgtcgttc
     9781 tcggcgaccg tggcgtcgac ccgctcctcc caggtcgtcg ccagcgccga gagcggcgcc
     9841 gtgtcgatcg ggacctccag caggcggccg agccgttcga cgagggccag ggcggccttc
     9901 ggattggggc ccgcggggag gtagtgcggg accgccgccc acaggctgag cgcccgcagg
     9961 ccggcgcggt tcgccacgtc ctgcagcact ccgacgatgc cggtcggccc ctcataccgc
    10021 gacgccgcga cgccgagcgc ctcggcctcc tcctgcgtcg gcgccgaccc ggccacccgg
    10081 atcgcgcgcg tgtgcggcat gtctgccagg aacccaccga gcgtgaccag cgtgtccgcg
    10141 tcgacctcgc ggccgaccgc gacgatcgcc tcgcagaacc cgcgccactt gttgttcggc
    10201 tcgatgccgg tgaacagcac cacgtcacgg gtaccggcgg acgcgtggaa gaagtcgttg
    10261 cgcggccact cgatgtgccg cgtcacgccg tcgatcaggc gcaccgtcgg ccggttcacc
    10321 tggaagtcga agaactcctc cgggtcgatg gtggcgaacc gtgtcgcccc ccacgcctcg
    10381 cgcaggaacc gcgccgcccc gctggccgac tccccgccgt cgttccatcc cgcgaaggcg
    10441 cagatgagcg ccggttctcg cagggttgga cggctctcga acgtgacggc gtccaccccc
    10501 caagcttccc acaccgcctg cctttcgccc ggcgtgaaga tcacccgctg caggcgttcc
    10561 tgtcagtgag gagctggagc ggcggccgct aggatcggtg gcgtgagcga ggaagcgggg
    10621 cgccggccga gggtcacgca gaagccgccg cggccgcgag acgatctggg gcggccgctg
    10681 ccccaccacg cgccgaacgc cctcgagctg gaggacttcg acgcgctctc gtgggaggag
    10741 aaccatcgcc tgggcgtcga gcacttcaac gcggggcggt tcttcccggc acacgaggcc
    10801 tgggagacgg cgtggaagca gctgaagggc acgcccgacg aggagttctt caagggcctc
    10861 tcccagctcg gcgcgggcta cgtccactac ctgcgcggga actcgcacgg ggcgttcacc
    10921 ctgttgcgcc gggcggccgg gcggatcgcc cgattcccgg ccggtcacca cggcgtggac
    10981 acggaccggc tcgtcgcggc ggcgctggcc accgccgacc gagtcgagga gtaccggacg
    11041 gtcgacccgg acgacctcga cccgccgcgg atctgacggc gatgcgcccg ggcgcgcgcc
    11101 cttcgagcgc cggcgcggcg atcgcctgat cagcaccgac ccggcacgcc tcgacctggg
    11161 cctggtccac gacgtcctgt cgaaccgttc ccacttggcg gccgggatcc cgcgcgagcg
    11221 cctggccccg acggtggctg aaggacgggg aggcgcggct gcgggtatcc tcgccccttc
    11281 gcaaccggtt accggaaggt aggttgagcc catgaacgtg gtcgtgctga cgaagtacgt
    11341 ccccaacccg gagggcgtgc cggagatggg ggacgacttc ctgctgaagc gggagggcgt
    11401 ggagggcgcg ctcgacccgg gggacgagtt tggcgtcgag gccggcctgc agctggccga
    11461 ggcgtccggc ggcgaggtca ccgtggtctc catgggcccc gagcccgcga tggggggcgt
    11521 gcgcaaggcg ctctcgatgg gtgcggcgaa gggggtcctg gtcaccgacc cgaagctccg
    11581 ggggtcggac gtgctcgtca ccgcgcgcgt cctggcggcc gccgtgaagc gcctcgagcc
    11641 ggacatcgtg gtcgccggcg tcgagtccac cgacgggtac accggcaccc tgccgatggc
    11701 cctcgcggag ttcctgggct ggccgaacgt gacgttcgcg cggcacctcg aggcgggcga
    11761 cgggtcggtg aaggtcgagc gccagaccga gaccggctac gacgtggtcg agtcgccgct
    11821 cccggccgtg gtgaccgtca cggcgggcgc caacgcgccg cgctacccga cgctgaaggg
    11881 gatcatggcc tccaagcaga agccggtgga gcagctcgcc gcgggcgacc tcgggctgtc
    11941 t
//
LOCUS       DASPWO010000131         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_869694, whole genome shotgun sequence.
ACCESSION   DASPWO010000131 DASPWO010000000
VERSION     DASPWO010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869694"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(404..1036)
                     /locus_tag="VEO00_03065"
     CDS             complement(404..1036)
                     /locus_tag="VEO00_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57017.1"
                     /translation="MPSLEEALTGALEAALESEAPPQAPSIGDLVELLGGRGRGGGTT
                     RLAGELGVSPRTVQRWLRAERGEGGETRAAATARVRGLTARQRGIADLIRGLWTDRDR
                     RIDVLERRLRGRKLVVSWDITWEPYPEPGDVRREPAPPWRDGGRFGPLVIPEHRAAPI
                     IRDGIEVLRSGDVDALDRAAGRLAGELAGAVGLPEEAEASEAHDLTISFE"
     gene            complement(1036..2658)
                     /locus_tag="VEO00_03070"
     CDS             complement(1036..2658)
                     /locus_tag="VEO00_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57018.1"
                     /translation="MASAFGWAFADAPVGACWVCDGPCHTRGPDGRPVHPLCWGKQPS
                     TPAAAAGEQLELADAGEEDQAVTATEHPRPEPHPEPRPGPRVPAALDAAGLHLPDGRV
                     LPVADRSTGAAIAAAAAGAGVRDVWLHAGVTRALGLEPARLQAQATGSPWLASAGEWY
                     PTQDRLTSMVVYRHGAEHEVTRLHVVAPGSRLGSLAGARDGAELLRLVLRFEALTGAR
                     WDGSASQTTAHLRRALVRGARGMSPGAYDPAELLPEPLARGVARAPSWYRALTPEEAA
                     RYPFVVLVDQRANYLAVLDSELGAKAPQHIPGPVELASLPKAGWARVRAGAWPDPQTF
                     DPLGALRRRADDAGAFWAELHVLRYAAGASVPVEVLEAWCFPAGARRFLAPVKLRLKE
                     ALYALEEAPEQEREALRPLVKAMYSEFVGWLASAEYHDRGDPLWRPDWHDAILSNAAV
                     VTSRKARKAPGRIAAARTDALGVLMERDDPAEAAALLGSRLGERLGDFKAEGAWPSAL
                     HVLEPAIAGDPDGATLEIRRRVHDWQRGRGGR"
     gene            complement(2688..2900)
                     /locus_tag="VEO00_03075"
     CDS             complement(2688..2900)
                     /locus_tag="VEO00_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57019.1"
                     /translation="MAEATCEHVREVQALRKFMQDEAKALRAMADLLEKRALHFDGSM
                     VEFARDAAHGYRVRAEDLEMRSQVPG"
BASE COUNT          371 a         1185 c         1112 g          403 t
ORIGIN      
        1 gctttggagg agttcgctcg ataaaggtag ggcaacgtca ggtcgcgaac gagtagctag
       61 aaatgggggg gctacagctc gccgtcgtga tggacttagc ggccgtagcg ctcatgcgcc
      121 gcctgcttgg agatgcctcc caggcctccc cgatgcgctc ccagctcacc gaccactaag
      181 cgagacgaag ctgccgaacc aagatgagca tggccgcgcc gatctcggcg cgggcgtccg
      241 ccagcgcgcc cagcgtgtac agcggacttt ggaggctggt cgttgcgacg ttgaaccgct
      301 cgctcgcctc ggcgagatgc tcccgcgggt cgatgtcgtc acagtcccgc tgcgtgggcc
      361 ggagccgaag gcggtcggga gcgtccagct ctacagctct ccgtcactcg aagctgatcg
      421 tcaagtcgtg cgcctcgctg gcctctgcct cttcgggaag gccgacggcg ccggccagct
      481 cccccgccaa ccggccggcc gcacggtcga gcgcgtccac gtcgccgctg cggagcacct
      541 cgatcccgtc gcggatgatc ggcgcggcgc ggtgctcggg gatcaccagg ggtccgaacc
      601 ggccgccgtc gcgccacggc ggcgccggct cgcggcgcac gtcgcccggc tccgggtacg
      661 gctcccaggt aatgtcccag ctcaccacca gcttgcggcc ccggagccgg cgctccagca
      721 catcgatgcg gcggtcccgg tcagtccaga gcccgcggat caggtccgcg atgccccgct
      781 gcctggccgt gagcccgcgg accctggccg tggccgcggc ccgggtctct cccccttcgc
      841 cgcgctcggc ccggagccag cgttggacgg ttcgcgggga cacgccaagc tcgccggcga
      901 gccgggtggt cccgccgccg cggccccggc cgccgagcag ctcgacgaga tcgccgatcg
      961 acggcgcctg cggcggcgcc tcgctctcca gggccgcctc tagggcgccg gtgagcgcct
     1021 cctcgagcga cggcactagc ggccacctct cccccgctgc cagtcgtgga cgcggcggcg
     1081 gatctccagc gtggcgccgt ccgggtcgcc ggcaatggcc ggctccagca cgtgcagcgc
     1141 gctcggccag gccccctcag ccttgaagtc tccgagccgt tccccgagcc ggctaccgag
     1201 cagcgccgcg gcctccgccg ggtcgtcccg ctccatgagc acgccgagcg cgtcggtgcg
     1261 ggccgccgcg atccggcccg gcgccttgcg cgccttgcgc gaggtgacta cagcggcgtt
     1321 gctcaagatc gcgtcgtgcc agtccggccg ccacagcggg tcgccgcggt cgtggtactc
     1381 ggcgctcgcc agccagccga cgaactcgga gtacatcgcc ttgaccagcg gccggagcgc
     1441 ctcgcgctcc tgctctggcg cctcctccag cgcgtacagc gcttccttca acctcagctt
     1501 gacgggtgcc aggaaccgcc gcgcgccggc ggggaagcac catgcctcca gcacctccac
     1561 cggcacgctg gcgccggcgg cgtagcggag cacatgcagc tccgcccaga acgcgccggc
     1621 gtcgtcagcg cggcggcgca gggcgccgag cgggtcgaac gtctgcgggt ccggccaggc
     1681 gccggcgcgc acgcgcgccc atccggcttt ggggaggctg gccagctcga cgggaccggg
     1741 gatgtgctgg ggggccttgg cgccgagctc ggagtcgagc acggcaaggt agttggcgcg
     1801 ctggtcgacc agcacgacga acgggtagcg cgccgcctct tcgggcgtca gcgcccggta
     1861 ccagctcggc gcccgggcca cgccgcgcgc gagcggctcg gggagcagct cggcggggtc
     1921 gtaggcgccg gggctcatgc ccctggcgcc gcgcacgagg gcccggcgca ggtgggcggt
     1981 ggtctggctg gcggagccgt cccagcgggc gccggtcagg gcctcgaagc ggagcaccag
     2041 gcggagcagc tcggcgccgt cgcgggcgcc ggcgaggctg ccgagccggc tgccgggtgc
     2101 cacgacgtgc aggcgtgtca cctcgtgctc ggcgccgtgg cggtagacca ccatgctcgt
     2161 tagccggtcc tgggtcgggt accactcccc cgccgacgcc agccacggcg agccggtcgc
     2221 ctgcgcctgc aagcgggccg gctccaggcc gagcgcccgg gttacccctg cgtggagcca
     2281 cacgtcccgg acaccggcgc cggcggccgc cgcggcgatg gcggcgccgg tggagcggtc
     2341 cgccaccggg agcacgcggc cgtctggcag gtggagcccg gcggcgtcca gcgcggccgg
     2401 gacgcgcggg cccggccgcg gctcggggtg cggctccggg cgcgggtgct cggtggcggt
     2461 cacggcctgg tcctcctcgc cggcgtcggc cagctccagc tgctcccccg ccgcggcggc
     2521 gggcgtgctc ggctgcttgc cccagcacag cggatgcacc gggcgtccgt ccgggccgcg
     2581 cgtgtggcag gggccgtcgc agacccagca ggcgccgacc ggagcgtccg cgaaggccca
     2641 cccgaaagca gacgccatcg aggcgctcgc gggcatcccg tcgagcgtca gccgggaacc
     2701 tgactccgca tctccaggtc ctccgctcgc accctgtacc catgcgccgc gtcccgcgcg
     2761 aactccacca tgctcccgtc gaagtgcagc gcccgcttct ccagcaggtc cgccattgcg
     2821 cgaagcgcct tcgcctcgtc ctgcatgaac ttgcgaagcg cctgcacctc gcggacgtgc
     2881 tcgcacgtcg cttccgccat catcaccctc ccacgtctgc gatgacggta ccgggggtac
     2941 cgggggtacc tccccggtca gggcgtcgcc gcctacctca gcagggggta ccgccctggt
     3001 cagggcgtcg ccgcccacct cgggcgccgg cgccggctgc gagccgttga cctgcccggt
     3061 acccccggta c
//
LOCUS       DASPWO010000132         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_191764, whole genome shotgun sequence.
ACCESSION   DASPWO010000132 DASPWO010000000
VERSION     DASPWO010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191764"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..671
                     /gene="moaC"
                     /locus_tag="VEO00_03080"
     CDS             168..671
                     /gene="moaC"
                     /locus_tag="VEO00_03080"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HYU57020.1"
                     /translation="MSASEFTHLDPLGRARMVDVTSKEATHRRAVARCRVNMESDTAS
                     KIASGAISKGDVLAVARVAGIQAAKQTPNLLPLCHPLLVGSVFVNFRIEDSYVEVETQ
                     VETVDRTGVEMEALTACAVAALTVYDMCKSVDRGMTIGDLALWEKTGGRSGTFRRTPA
                     VDDELSL"
     gene            864..1433
                     /locus_tag="VEO00_03085"
     CDS             864..1433
                     /locus_tag="VEO00_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57021.1"
                     /translation="MILAVLWAAVLVPPALRSRSEGRRGETIGPLNFRLGSGGPGGGR
                     SLSSMFNRPASGPSGPGGFRPAAYPTRTASPAGAPARAGAPPRPPGEMTPAQKRRRDV
                     LLMLLAAVGLTFVMAAFTGVVALWLFHLLADALLGGYLYLLLQLKQRGGIAGTRSALP
                     PPIPLERPRQARHVAHRSPELLLRRTAGQ"
     gene            1460..2098
                     /locus_tag="VEO00_03090"
     CDS             1460..2098
                     /locus_tag="VEO00_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HYU57022.1"
                     /translation="MEPDRSAEIADAGELAREALEEKHKAREVTLSASRRAIRSCATA
                     IRAVHRREFPEARALIAEAAAFLAEADAALERHDDIRYAGFLHDAKKEFAEANLTLAF
                     VAALPMPAAEELRVEVQAYLNGMAEAASELRRQVLDCLRRDEVGEAENLLAVMDEVYG
                     LLITVDYPEALTAGLRRSTDALRGVVERTRGDVTNALVAARVKDALDSRKPD"
     gene            2132..2208
                     /locus_tag="VEO00_03095"
     tRNA            2132..2208
                     /locus_tag="VEO00_03095"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2166..2168,aa:Ala,seq:cgc)
BASE COUNT          357 a          844 c          787 g          377 t
ORIGIN      
        1 acgacgacct cgccgacgag atcgagcgcg ccgtgggcac gaagtgggcc gggcaccgca
       61 tcggccaggt gaacttcgtc cggcccgggc gttcgatgag ccagatcggc ggctgaccct
      121 cccgcccctg tcgcctgacc aggggcaatc cctagactgg ccatcccatg tccgcctccg
      181 agttcaccca cctcgatccg ctcggacggg cacggatggt cgacgtcacc tccaaggagg
      241 ccacccatcg ccgggcggtg gcccggtgcc gggtcaacat ggagtcggac accgcctcga
      301 agatcgcatc gggggcaatc agcaagggcg acgtcctcgc ggtcgcccgg gtggcgggga
      361 tacaggcggc caagcagacc ccgaacctgc tgccgctctg tcaccccctt ctggtgggct
      421 cggtgttcgt gaacttccgg atcgaggaca gctatgtcga ggtcgagacc caggtcgaga
      481 ccgtcgaccg gaccggggtg gagatggagg cgctcaccgc ctgcgccgtc gccgcgctga
      541 ccgtctacga catgtgcaaa tcggtcgatc ggggcatgac catcggcgac ctggccctct
      601 gggagaagac cgggggccgc tcgggcacgt tccggcggac ccccgccgtc gacgacgaac
      661 tctccctctg acctgggggt tcgcggcaaa accgcaggtc agagggggta aaatcgcccg
      721 tctagtactt gatctgcccc acccggactc cgtagtgttg tcgctgacgt cacgggcgga
      781 catccacgtc aagcagcttg cggcttgtta ccgtccccaa acacgctgcg tggttgagga
      841 gcaggttgac gatcacagtc cttttgatcc tggcggtgct gtgggcggcg gtgctggtgc
      901 cgcccgctct gcgatcccgc tccgagggcc gccggggaga gacgatcgga cccctcaact
      961 tccgcctcgg ctccggtggg cccggcggcg ggcgttccct gagctcgatg ttcaaccggc
     1021 ccgccagtgg tcccagtggt ccgggcggct tccgtcccgc cgcctacccg acccgtaccg
     1081 ccagcccggc gggggcgccc gcccgagccg gcgccccgcc ccgacccccg ggggagatga
     1141 ccccggcgca gaagcggcgc cgagacgtcc tcctgatgct cctggccgcg gtgggcctga
     1201 cgttcgtgat ggccgcgttc accggcgtcg tggcgttgtg gctgttccac ctcctcgctg
     1261 acgccctctt gggcggatac ctctacctgc tcctgcagct gaagcaacgc ggtggaatcg
     1321 ccgggacgcg gtcggcgctg ccaccgccga tccccctcga gcggccccgc caggcccgcc
     1381 acgtcgccca ccgctctccc gagctcctcc tccggaggac cgcggggcag tagcggcggc
     1441 gccgtagcct ggttccggga tggaaccgga ccggtcggcc gagatcgccg acgcgggcga
     1501 gctcgcccgc gaggccctcg aggagaagca caaggcccgt gaggtcaccc tcagcgcgtc
     1561 gcggcgggcc atccgctcct gtgccaccgc catccgcgcc gtgcaccggc gcgagttccc
     1621 cgaggcccgg gcgctcatcg ccgaggcggc ggccttcctc gccgaggccg acgctgctct
     1681 cgagcgccac gacgacatcc gctacgccgg cttcctccac gacgccaaga aggagttcgc
     1741 cgaggccaac ctgaccctcg cgttcgttgc cgcgctgccg atgccggcgg ccgaagagct
     1801 gcgggtcgag gtgcaggcgt acctcaacgg catggccgag gcggccagcg agctgcgccg
     1861 tcaggtgctc gattgcctgc gacgcgacga ggtcggtgag gccgagaacc tgctggcggt
     1921 gatggacgag gtgtacggcc tgctcatcac ggtggactac ccggaggcac tcaccgccgg
     1981 tctgcgccgc tcgaccgacg cgctccgcgg cgtcgtcgaa cgcacccgcg gcgacgtcac
     2041 gaacgcgctg gtcgcggcgc gcgtcaagga cgcgctcgac agccggaagc cggactgatt
     2101 ccgcgaggcc tttggtaccc tggatcgctc cgggggtgta gctcagcctg gtagagcgct
     2161 acgttcgcaa cgtagaagtc gtgggttcaa gtcccatcac ctccaccaac tgataatcat
     2221 tctcacctca gatgtgagag cgacctcgga aatacgcact acccctggtt caaagtccac
     2281 ttagctgttc gcacattgtg cgacgcctac atctgttatg ggcttcaatg cgtttacgtg
     2341 tgcgcgactt cgtgttgcaa tgagt
//
LOCUS       DASPWO010000133         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_532026, whole genome shotgun sequence.
ACCESSION   DASPWO010000133 DASPWO010000000
VERSION     DASPWO010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532026"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VEO00_03100"
     CDS             complement(<1..1268)
                     /locus_tag="VEO00_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU57023.1"
                     /translation="MQRPETHYATSGDIHIAYHVVGDGPLDLVYVAEFWNSIEAQWEE
                     PRFEAFLRRLASFSRLICFDQRGTGLSDPVALSELPTLEEWMDDVRAVMDAVGSRRAA
                     LLGSGGGGLMSMLFAATYPEQTHALILVNSTAKVTRTDDYPQGNSDDFEDRIVRDLRF
                     GWGRGVLLETVGPSMAGDAAFRDWWARYQRLGSSPGTVLVMRGMLQQTDVRHVLPSIR
                     VPTLVLHRQDNRLLDVAHGRYLGEHIPGARYVEVPGDDHFPFVGDTAPILDEIEEFLT
                     GVRPARAAARLLATVLFTDLVGSTETAASVGDSRWRTLLQAHHDLVRREVERARGREI
                     DRAGDGVLATFDGPARAIECARAIRDGVRALGLEVRAGLHTGEVELLEEGIGGLAVHI
                     GARVSALAGPGEVLVSSTVKDLVAGSGIEF"
     gene            1344..>2225
                     /gene="cofG"
                     /locus_tag="VEO00_03105"
     CDS             1344..>2225
                     /gene="cofG"
                     /locus_tag="VEO00_03105"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HYU57024.1"
                     /translation="MATAFDHGVRRALARAGAGKSLSLDEATSLMAARGHALKDLMAI
                     AATLRDRAHGRTVTYSRKVFVPLTMLCRDHCHYCTFAKPPARLEAPYLTPEEVLAIAE
                     AGRRAGCKEALFTLGDRPEDRYPAAREWLAERGYASTLDYLRSVAIRVIEETGLLPHL
                     NPGVMSWEQLARLKQVSGSMGLMLETSSDRLSARGGPHFGSPDKVPAVRLSTIEDAGK
                     LSIPFTTGILVGIGETARERAESLLAIRDLHRRYRHVQEVIVQNFRSKPGTAMRDHPE
                     PAVEEFLAAVATARVILG"
BASE COUNT          308 a          839 c          778 g          300 t
ORIGIN      
        1 tcgaactcga tcccggaccc cgccacgaga tccttgacgg tcgaggacac caggacctcg
       61 cccggtccgg cgagcgccga gacgcgggcg ccgatgtgca ccgcgaggcc cccgatgccc
      121 tcctcgagca gctcgacctc ccccgtgtgc aggccggccc gaacctcgag gccgagcgcg
      181 cgcacgccgt cccggatcgc ccgcgcgcac tcgatcgcgc gcgcggggcc gtcgaacgtt
      241 gcgagcaccc cgtcgccggc gcggtcgatc tcccgtccgc gggcgcgctc cacctctcgc
      301 cggaccaggt cgtggtgcgc ttggagcagc gtccgccagc ggctgtcacc gaccgacgcg
      361 gccgtctcgg tggagcccac caggtcggtg aacagcacgg tggccagcag ccgggcggcg
      421 gcgcgcgcgg ggcgcacgcc ggtcaagaac tcctcgatct cgtcgaggat cggcgccgtg
      481 tccccgacga acgggaagtg gtcgtcgccc ggcacctcga cgtaccgcgc cccggggatg
      541 tgctcgccga ggtagcggcc gtgtgccacg tccagcagcc ggttgtcctg ccggtgcagg
      601 acgagggtcg gcacccggat cgagggcagc acgtgacgca cgtcggtctg ctgcagcatc
      661 ccgcgcatca cgaggaccgt ccccgggctc gagccgagac gctggtacct cgcccaccag
      721 tcgcggaagg cggcgtcccc ggccatgctc ggcccgaccg tctccagcag gaccccacgc
      781 ccccagccga agcgcaggtc ccggacgatg cggtcctcga aatcgtcgga gttcccctgc
      841 gggtagtcgt cggtgcgcgt gaccttggcc gtcgagttca ccaggatcag cgcgtgcgtc
      901 tgctccgggt acgtcgccgc gaacagcatg ctcatcagcc cgccgccgcc ggacccgagc
      961 agcgcggccc ttcgcgagcc gaccgcgtcc atcacggcgc gcacgtcgtc catccattcc
     1021 tcgagcgtcg gcaactcgga gagcgcgacc ggatccgaca ggcccgttcc ccgctgatcg
     1081 aagcagatca gccggctgaa cgaggccagc cgccgcagga acgcctcgaa gcgcggttcc
     1141 tcccactgcg cctcgatcga gttccagaac tcggcgacgt agaccaggtc gagtggcccg
     1201 tcgccgacca cgtggtaggc gatgtggatg tcgccgctcg tggcgtagtg cgtctccggc
     1261 cgctgcacgc ggccagtctc gcgccgccgg tgcgggcccg gcaacacgca accgatcggg
     1321 gggccggtgc tacgctcgcc cggatggcga cggccttcga tcatggcgtg cggcgcgccc
     1381 tcgcccgagc cggcgccggc aagtccctct ccctcgacga ggcgacctcg ctgatggccg
     1441 cgcgcggcca cgcgctgaag gacctgatgg cgatcgccgc cacgctgcgc gatcgggcac
     1501 acggccgcac cgtcacctac tcgcgcaagg tgttcgtccc gctgacgatg ctctgccgcg
     1561 accactgcca ctactgcacg ttcgccaagc ccccggcgcg gctcgaggcc ccgtacctca
     1621 cgcccgagga ggtgctggcg atcgccgagg ccgggcggcg cgccgggtgc aaggaggcgt
     1681 tgttcacgct cggggaccga cccgaggacc gctacccggc cgcgcgggag tggctcgccg
     1741 agcgcggcta cgcctccacg ctcgactacc tccgctcggt ggcgatccgg gtgatcgagg
     1801 agaccggcct gctgccgcac ctcaacccgg gcgtgatgtc ctgggagcag ctggcccggc
     1861 tgaagcaggt gtcgggttcg atggggctga tgctcgagac gtcgtccgac cggctgtcgg
     1921 cgcggggcgg cccgcacttc ggctcgccgg acaaggtgcc ggccgtgcgc ctctccacga
     1981 tcgaggacgc gggcaagctg tcgatcccgt tcaccaccgg gatcctcgtg gggatcggcg
     2041 agaccgcccg ggagcgcgcc gagtctctgc tcgcgatccg cgacctccac cgtcgctacc
     2101 gccacgtcca ggaggtgatc gtgcagaact tccggtcgaa gcccgggacg gcgatgcgcg
     2161 accatcccga gccggcggtg gaggagttcc tggcggcggt ggcgacggcg cgcgtgatcc
     2221 tcggg
//
LOCUS       DASPWO010000134         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_192124, whole genome shotgun sequence.
ACCESSION   DASPWO010000134 DASPWO010000000
VERSION     DASPWO010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192124"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VEO00_03110"
     CDS             <1..946
                     /locus_tag="VEO00_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYU57025.1"
                     /translation="AHARPPGPQDDPGTLARSLPRLPLPEADDLPVVVAARMSMSFPG
                     LLSMVPLWSIDRTAPGHPVDRVWFSDGGIASNFPVHLFDAPLPRWPTFAINLGPFRTG
                     EGPHADPARDVWMPDASHMGVQPRWIRIEGLGGFARAAFDTLQNWADNAQIRVPGYRD
                     RVVLVKHTEEEGGIKLDMPPPRILALAARGAAAGDRLVRRFAEPPSGPRALSWDSHRW
                     VRYRTAMALLESWLDGLTEGYGYSPQAGALPYRDLIARELDAPPNHYAWARLAQRAYA
                     MEATGELLDLVLGWRGNPETFAEGAPGPMPLLRITPAF"
     gene            complement(947..1576)
                     /locus_tag="VEO00_03115"
     CDS             complement(947..1576)
                     /locus_tag="VEO00_03115"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1277..1279),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYU57026.1"
                     /translation="MGAGEPRRSRRPPGCRPPPPRRGAPGRPPGAWVVSDSSVRGLVR
                     SRVVARRSRLLDVLSEGTLAPPFTLPDQDGTPVSLEDHRGSWVVLWWFPKAATAGUTR
                     EGQGFRDRAPDFRAAGAVLLGASFDTPEDNRAFRASHGFPFPLLSDRDHAVGRLYETE
                     RHPDEDAPHWAKRRTYLIDPRGVIRKAYRVRDIPAHPDEVLADLRALQD"
     gene            1465..>2204
                     /locus_tag="VEO00_03120"
     CDS             1465..>2204
                     /locus_tag="VEO00_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57027.1"
                     /translation="MNRTRPTLLAAVLALLAVAAGGGSPAAGATAAAPPLPPVLSITA
                     LGIVQQHPSVYGRDGTFSALVGGRSVWSFGDTPLAVPGVSGDHWVDNSMAWTADLDAS
                     DGIALEGNRLDSTGAPAEFLPLTAAERAYNDAHDVDHCTADPCGAEFALWAGPVVPDP
                     ARNRILHLYTEIWRVPGREGWKGVGGGIAVQGANGKVTRPVQDPDSRTPTLMWTGDDV
                     QFNAASVVVGQMLYAYGCRAGFLVMHCKV"
BASE COUNT          292 a          803 c          807 g          302 t
ORIGIN      
        1 cgcccacgcg cggccgcccg gcccgcagga cgaccccggc acgctcgccc gatcactccc
       61 gcggctgccg ctccccgaag cggacgacct cccggttgtg gtggctgccc ggatgagcat
      121 gtcgttcccg ggactcctgt ccatggttcc gctatggtcc atcgaccgta ccgcgccggg
      181 ccatccggtc gatcgggtgt ggttctccga cggcgggatc gccagcaact tcccggtgca
      241 cctgttcgac gctccacttc cccggtggcc gaccttcgcc atcaacctcg gcccgttccg
      301 tacgggggaa ggcccgcacg ccgaccccgc ccgcgacgtc tggatgccgg acgccagcca
      361 catgggcgtg cagccccgat ggatccgcat cgagggcctc ggcgggttcg cgcgggcggc
      421 cttcgacacg ctgcagaact gggcggacaa cgcgcagatc cgggtccccg gctaccgcga
      481 ccgcgtcgtg ctcgtgaagc acacggagga ggagggcggg atcaagctgg acatgccgcc
      541 gccccggatc ctggcgctgg cggcacgcgg cgcggcggcc ggcgaccggc tggtcaggcg
      601 gttcgccgaa ccgccctcgg gaccgcgggc gctctcctgg gacagccacc gctgggtccg
      661 gtaccgcacc gcgatggcgc tgctggaatc gtggctggac ggcctgacgg aggggtacgg
      721 gtactcgccg caggccgggg cgcttccgta ccgggacctc atcgcgcgcg agctcgacgc
      781 cccgccgaac cactacgcct gggcgcgcct ggcgcagcgg gcctacgcga tggaggccac
      841 cggcgaactg ctcgacctgg tgcttggatg gcgggggaac ccggaaacgt tcgccgaggg
      901 ggctcccgga ccgatgcccc tcctgcggat caccccggcg ttctgatcag tcctgcagcg
      961 cgcgcaggtc ggcgagcacc tcgtcgggat gggccgggat gtcgcgcacc cggtaggcct
     1021 tgcggatcac gccccgcggg tcgatcaggt acgtcctgcg cttggcccag tgcggcgcgt
     1081 cctcgtcggg atggcgctcg gtctcgtaga gccgtccgac ggcatggtcc cggtccgaga
     1141 gcagcgggaa ggggaagccg tggctcgcgc ggaacgcccg gttgtcctcg ggcgtgtcga
     1201 aggaggcccc caggagcact gccccggcgg cccggaagtc gggggctcga tcacggaacc
     1261 cctgtccctc gcgcgttcag cccgccgtgg cggccttcgg gaaccaccag aggaccaccc
     1321 aggacccgcg gtggtcctcg agcgataccg gtgtgccgtc ctggtcgggg agggtgaacg
     1381 gcggggcaag cgtcccctcg ctcagcacgt caagtagtct ggaccgtcgc gccacgacgc
     1441 gcgaacgaac gagccctcgc acagatgaat cggacacgac ccacgctcct ggcggccgtc
     1501 ctggcgctcc tcgccgtggc ggcggggggc ggcagcccgg cggccggcgc gaccgccgcg
     1561 gctcccccgc tcccacccgt cctgtccatt accgcgctcg gcatcgtgca gcagcaccca
     1621 tcggtgtacg ggcgtgacgg caccttcagc gcgctggtcg gggggcgctc ggtctggtcg
     1681 ttcggcgaca ccccgctcgc ggtccccggc gtgtccggtg atcactgggt ggacaactcg
     1741 atggcctgga cggccgacct ggacgcctcc gacgggatcg cgctggaggg gaaccgcctc
     1801 gactcgaccg gggcgccggc ggagttcctg ccgctcaccg cggccgagcg cgcctacaac
     1861 gacgcccacg acgtggacca ctgcacggcc gacccgtgcg gcgcggagtt cgcgctgtgg
     1921 gccgggcccg tggtccccga cccggcgcgc aaccggatcc tgcacctcta caccgagatc
     1981 tggcgggtcc cgggacggga gggctggaag ggggtcggcg gcgggatcgc ggtgcagggc
     2041 gcgaacggga aggtgacgcg tcccgtgcag gaccccgaca gccggacgcc gacgctgatg
     2101 tggaccggcg acgacgtcca gttcaacgcg gcgtcggtcg tcgtcgggca gatgctctac
     2161 gcgtacgggt gccgggccgg gttcctggtg atgcactgca aggt
//
LOCUS       DASPWO010000135         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1123296, whole genome shotgun sequence.
ACCESSION   DASPWO010000135 DASPWO010000000
VERSION     DASPWO010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123296"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VEO00_03125"
     CDS             complement(<1..152)
                     /locus_tag="VEO00_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HYU57028.1"
                     /translation="MIELSLVGVRVELPTNQPIVLLKEKEGERFLPIWIGAMEATAIA
                     FALQGIV"
     gene            complement(171..782)
                     /gene="hpt"
                     /locus_tag="VEO00_03130"
     CDS             complement(171..782)
                     /gene="hpt"
                     /locus_tag="VEO00_03130"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HYU57029.1"
                     /translation="MEPGVVSVEGVEVLFTEEQIRDRVRALGEAITADYAGRAPVLIS
                     VLKGGTVFLADLLRRIHLPVRIDFMSISQYEEDARASGVVRIVKDLDQDIGDEDVVVV
                     EDIIDTGLTLSYLLTSLWSRAPRSLEVCTLLDKSVRRIAPLEIRYTGFDCPDAFVVGY
                     GLDLQERYRNLPFIVSLPRERLADADPDLLLPYLAAPGQGRGE"
     gene            complement(860..2146)
                     /locus_tag="VEO00_03135"
     CDS             complement(860..2146)
                     /locus_tag="VEO00_03135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HYU57030.1"
                     /translation="MSSRRAFVACLLALTAAATLVAPAVRAQSPTPLPSEVPPPTPVP
                     GGTTPSPFPSVLITPSPDVTPPPVRAAAALVEDLDTGQMLYAMNPRAHRPIASLTKIM
                     TALLVLERTDPGDVVTASPTAAAAGGAEFGLVPGERHSVRQLLYALLLQSANDVAVAL
                     AEHVSGNVEAFVALMNSRARQLELADTRFASPNGLDDTGYSSAADLAALARAAMEQER
                     FAAVVAARARDIPVEGDGSRHVQNRNALLWLYPGTVGVKTGYTAAAGFCLVAAAERDG
                     ERILTVVLGEPEAAWDDGAALLDHTFLAFDRVELVEAGDRFHDVHVRGEVYRAVAGAS
                     LVRMVPVPAGPVTREVRLSGVLRPPLDPGDRLGRVVVMAGDMPMGSVPLVVGPPPPAP
                     PPPPPPGPDDLSAIADRLASALVRLVRALFEGDWGG"
BASE COUNT          353 a          841 c          873 g          293 t
ORIGIN      
        1 acgatcccct gcagggcgaa ggcgatcgcg gtggcctcca tggcgccgat ccagatcggg
       61 aggaagcgct cgccctcctt ctccttgagc agcacgatgg gctggttcgt cggtaactcg
      121 acgcgcacac cgaccaggct cagctcgatc atgggccgag cctcaatcta tcactccccc
      181 cttccctggc ccggggcggc caggtagggc agcaggaggt cggggtcggc gtcggcgagt
      241 cgctcccggg gcagggagac gatgaacggc aggttccgat agcgctcctg caggtccagg
      301 ccatagccga ccacgaaggc gtcggggcag tcgaagcccg tgtagcggat ctcgagcggc
      361 gcgatgcggc ggaccgactt gtcgagcagc gtgcagacct ccagcgagcg cggtgcgcgc
      421 gaccacaggg aggtgagcaa gtaggacagg gtcagtccgg tgtcgatgat gtcctcgacg
      481 acgacgacgt cctcgtcgcc gatgtcctgg tcgaggtcct tgacgatgcg caccacaccc
      541 gaggcgcgcg cgtcctcctc gtactgcgag atcgacatga agtcgatccg gaccggcagg
      601 tggatccggc ggagcaggtc ggccaggaag accgtcccgc ccttcaggac cgagatcagg
      661 accggcgcgc ggccggcgta gtcggcggtg atcgcctccc cgagggcccg gacgcggtcg
      721 cggatctgct cctcggtgaa cagcacctcg acgccctcga cgctcacgac gccaggctcc
      781 atcgcgcgat tctacggttc gtttcgctcg gggggttcgc tcgccggacg tggtctggcc
      841 cctctctcca cagggcgagc tatccccccc aatccccctc gaacagggcc ctgacgagcc
      901 tgaccagggc ggacgccagg cggtcggcga tcgccgacag gtcatcgggc ccgggtgggg
      961 ggggcggcgg cggggcgggc ggcggcggcc cgacgacgag cggcaccgaa cccatcggca
     1021 tgtccccggc catgaccacc acccggccga gccggtcgcc cgggtcgagg gggggccgga
     1081 gcaccccgga cagccgcacc tcgcgcgtca ccggaccggc cggcaccggg accatgcgca
     1141 cgagcgacgc cccggccacg gcccggtaga cctccccccg gacgtggacg tcgtggaaac
     1201 ggtcccccgc ctccacgagc tcgacgcggt cgaacgccag gaaggtgtgg tcgagcagcg
     1261 ccgcgccgtc gtcccacgcg gcctccggct cgccgagcac caccgtcagg atccgctccc
     1321 cgtcgcgctc ggcggcggcg accagacaga agccggccgc ggccgtgtag ccggtcttca
     1381 ccccgaccgt cccgggatac agccagagca gcgcgttgcg gttctgcacg tggcgcgacc
     1441 cgtcgccctc cacggggatg tcgcgcgcgc gggcggcgac gacggcggcg aagcgctcct
     1501 gctccatcgc ggcgcgcgcg agcgctgcca gatcggcggc cgacgagtac ccggtgtcgt
     1561 ccagcccgtt cgggctggcg aaccgcgtgt cggcgagctc cagctgccgc gcgcgcgagt
     1621 tcatcagggc gacgaacgcc tcgacgttcc cggagacgtg ctcggcgagt gccaccgcga
     1681 cgtcgttcgc cgactgcaac agcagcgcgt acagcagctg gcggacggaa tgccgttccc
     1741 ctggcacgag gccgaactcc gctcctccgg cagcggccgc cgtcggcgac gccgtcacca
     1801 cgtcgcccgg gtcggtgcgc tccagcacga gcagcgccgt catgatcttc gtcaggctgg
     1861 cgatcggccg gtgcgcccgc gggttcatgg cgtagagcat ctggccggtg tcgaggtcct
     1921 cgaccagcgc cgcggccgcc cgcaccggcg gcggcgtgac gtccggcgag ggagtgatga
     1981 ggaccgaggg gaacggagag ggcgtggtgc cgcccgggac cggcgtcggg ggcgggacct
     2041 cggatgggag cggcgtcggc gactgggcgc gcaccgccgg cgccaccagc gtggcggccg
     2101 cggtgagcgc gagaaggcag gcgacgaagg cgcgacggct cgacacggac gtgagggtac
     2161 gacgggccgg ccgggccgga gacgggtcag gcctgcagat gctgacggag gttcgagcgg
     2221 agcaatgcct gcttcagccg gcgcgacagc cgcgccaggt ccccgagcga ctcattcgcg
     2281 gagcggcggg cctcggggtt ccgctggcgg agcatcggca acacgatcgc ctcgaagaac
     2341 gccgcctcgc gctcggcgaa
//
LOCUS       DASPWO010000136         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_614301, whole genome shotgun sequence.
ACCESSION   DASPWO010000136 DASPWO010000000
VERSION     DASPWO010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614301"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VEO00_03140"
     CDS             <1..481
                     /locus_tag="VEO00_03140"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYU57031.1"
                     /translation="WITSHFVVTSDRLIHREGWFAKRSMEIPLERVNDVRFEQSILER
                     IVGAGDLRIESAGEYGQNAFTHIRHPERVQKLIFEMGEENQARMSRGESLGGPRDTDR
                     LDTGARTTERTEARTTEDLHTAGLTVPEQLERLAALRDRGVINEQEYAEEKARILGE"
     gene            486..1121
                     /locus_tag="VEO00_03145"
     CDS             486..1121
                     /locus_tag="VEO00_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU57032.1"
                     /translation="MEPPAFPLEVALRDGTTVLIRPIEPHDKQRMIDGFERLSEESRY
                     RRFLAPVTRLSEEQLTNLTEVDHVDHEALVALAADEAGQPGLGVARYVRLTDRPTVAE
                     AAVTVVDDAQGKGLGSVLLALLGQVARERGIERFRAYVLESNRPMLEILEQLGAEAHR
                     DSPGLLVVDAVLPTEPEDAPGEVARRVLRAVAQGKVGEVVPDAGLRRRPPG"
     gene            1131..1490
                     /locus_tag="VEO00_03150"
     CDS             1131..1490
                     /locus_tag="VEO00_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU57033.1"
                     /translation="MAVVRGLDHVYYWVADMDRSVAFYTDVIGLSLVRRDGANWAVFD
                     AGGRQFALHGAIEGRPLRPGGAAAVFAVDDLDQAMRALEGKGVVFDHVGEVQGYARFA
                     SFQDPDGNTAQLIEYAA"
     gene            1512..>2059
                     /locus_tag="VEO00_03155"
     CDS             1512..>2059
                     /locus_tag="VEO00_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYU57034.1"
                     /translation="MPDRDHAEALRAAAEASRAGGAEKYHARNAEQGKLFARERLRLL
                     LDDADGFVEDGLLVNALAGDLPADGVVTGVGTIAGRKVAVMTNDSTVKAGSWGARTVE
                     KIIRIIEWARRHERPIVYLVDSAGARITDQVDLFPGRRGAGKIFYNQVQASGKVPQVC
                     CLFGPSAAGGAYIPAFCDVVFMV"
BASE COUNT          295 a          713 c          770 g          281 t
ORIGIN      
        1 gtggatcacc tcgcacttcg tcgtcaccag cgaccggctg atccatcgcg aggggtggtt
       61 cgccaagcgc tcgatggaga tcccgctcga gcgggtgaac gacgtccggt tcgagcagtc
      121 gatcctggag cggatcgtcg gcgccggcga tctgcgcatc gagtccgccg gcgagtacgg
      181 ccagaacgcc ttcacccaca tccgccaccc cgagcgcgtc cagaagctga tcttcgagat
      241 gggggaggag aaccaggcgc ggatgtcccg cggcgagagc ctgggtggtc cgcgcgacac
      301 ggatcggctg gacaccggcg cgcggaccac cgagcggacg gaggctcgca ccaccgagga
      361 cctgcacacg gcgggcctga cggtgcccga gcagctcgag cgcctggcgg ccctgcgcga
      421 ccgcggggtg atcaacgagc aggagtacgc cgaggagaag gcgcggatcc tcggggagtg
      481 atctggtgga gcccccggcc ttcccgctcg aggtcgcgct gcgcgacggc acgacggtcc
      541 tgatccggcc gatcgagccg cacgacaagc agcggatgat cgacgggttc gagcgcctgt
      601 cggaggagtc gcgctaccgc cgcttcctcg cgccggtcac ccggctctcc gaggagcagc
      661 tcacgaacct gaccgaggtc gaccacgtcg accatgaggc actggtggca ctcgccgccg
      721 acgaggcggg gcagccggga ctcggcgtgg cccgctacgt ccggctgacc gaccggccga
      781 ccgtcgccga agctgccgtc accgtcgtcg acgacgcgca ggggaagggg ctcgggtcgg
      841 tgctgctggc cctgctggga caggtcgccc gcgagcgcgg catcgagcgc ttccgcgcgt
      901 acgtgctgga gtcgaaccgc cccatgctcg agatcctcga gcagctggga gcggaggcgc
      961 atcgtgactc gcccggcctg ctggtggtcg acgccgtgct gcccacggaa cccgaggacg
     1021 caccgggcga ggtcgcccgc cgggtcctgc gggcggtggc gcagggcaag gtaggcgagg
     1081 tggtgcccga cgccggcctg cgccgtcgcc cccccggcta gactcccgcg atggcggtcg
     1141 ttcggggcct ggaccacgtc tactactggg tggccgacat ggaccggtcc gtggccttct
     1201 acaccgacgt gatcggtctc tcgctcgtgc ggcgcgacgg cgccaactgg gcggtcttcg
     1261 acgccggcgg ccgccagttc gcgctgcacg gcgcgatcga gggccgcccg ctgcgccccg
     1321 gcggcgcggc cgccgtcttc gcggtggacg acctcgacca ggccatgcgc gcgctggagg
     1381 gcaagggggt cgtcttcgac catgtcggcg aggtccaggg ctacgcgcgc ttcgcgagct
     1441 ttcaggaccc cgacggcaac accgcccagc tgatcgagta cgccgcctga ttcggcgggt
     1501 acgcttgtcc catgccggat cgcgaccacg ccgaggcgct ccgggcggcg gccgaagcgt
     1561 cgcgggcggg cggggccgag aagtaccacg cgaggaacgc cgagcagggg aagctcttcg
     1621 cgcgcgagcg gctgcgcctc ctgctcgacg acgccgacgg gttcgtcgag gacgggctgc
     1681 tggtgaacgc gctggccggc gacctgccgg ccgacggcgt cgtcaccggc gtcgggacca
     1741 tcgccggccg caaggtcgcc gtgatgacga acgactcgac ggtgaaggcc gggtcgtggg
     1801 gcgcgcggac cgtcgagaag atcatccgga tcatcgaatg ggcgcggagg cacgagcgcc
     1861 cgatcgtcta cctggtcgac tcggccggcg cgcggatcac cgaccaggtg gacctgttcc
     1921 cggggcggcg cggcgccggg aagatcttct acaaccaggt ccaggcgtcc gggaaggtgc
     1981 cccaggtctg ctgcctgttc ggtccctcgg cggccggcgg cgcctacatt cccgcgttct
     2041 gcgacgtggt gttcatggt
//
LOCUS       DASPWO010000137        10065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_192573, whole genome shotgun sequence.
ACCESSION   DASPWO010000137 DASPWO010000000
VERSION     DASPWO010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192573"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VEO00_03160"
     CDS             <1..504
                     /locus_tag="VEO00_03160"
                     /inference="COORDINATES: protein motif:HMM:NF021900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HYU57035.1"
                     /translation="DLRLLSMGPRTGLAEIRLPEVQPGSSIMPGKVNPSVPEMVNMVC
                     FRVSGSEHTVALAVGAGQLDMSVMTPVIADELLEAQTLLAAACRTLAERCVAGIEADI
                     ERCRAYAEASLGLATALRPALGYARAADLAVEAMRSGRTLREIVSERGALPHEKLDEV
                     LDPARYA"
     gene            complement(495..860)
                     /locus_tag="VEO00_03165"
     CDS             complement(495..860)
                     /locus_tag="VEO00_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57036.1"
                     /translation="MMRAVFTPADDPNRTVGEVAFDGRAVTIASAPDEEARAALQRVF
                     RPAAIVVDDPALRPAGASGESVLQPGGQEWFRAAVAVRAAEEGLKGRLAPEGWRGGWD
                     PAAAYRTFREDLRRRAAQA"
     gene            complement(868..1662)
                     /locus_tag="VEO00_03170"
     CDS             complement(868..1662)
                     /locus_tag="VEO00_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57037.1"
                     /translation="MDVRETLGMRWIDSAIAEAAQYPMFFTAAWSAIRPNVGPTFLAS
                     AKVVRDLAAAAAEAGAPPDHAEWIRLARLQADEVAEVARVLRAFHENQAKAALVVHAL
                     ARAARGRPAGGSGIEEPPPKRGVPAWQPHARLELPGEGDEAAEALAAAAKRLGAPRPP
                     DALLALRPWPGYLTRAWRAGRRFTASDTFSKASGRIRRAIVEGVRRLPHVISLQWGAL
                     RDRGFSEEDRAAVGDALALYDAAMPGTLALVAYLWVAAGSPKGSRG"
     gene            complement(1740..2153)
                     /locus_tag="VEO00_03175"
     CDS             complement(1740..2153)
                     /locus_tag="VEO00_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYU57038.1"
                     /translation="MGGEAQLASVPLFSRCSRRDLKRVAGLASEVTFEAGDVIAEEGE
                     DGLSFYVVLEGEAKVTRRGKSVARLLPGSFFGEVSLLDGGPRTATVTAVTPMRCLSIL
                     RWDFRSLLEKEPALIPKILKELALRLRQTEKSVTG"
     gene            complement(2153..2953)
                     /locus_tag="VEO00_03180"
     CDS             complement(2153..2953)
                     /locus_tag="VEO00_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYU57039.1"
                     /translation="MFRKILVGTDGSDTATMAVAHALKLAEAIGAEVTAISVYATPQV
                     DAPEILSARQGDPTADIARAILRDVQNRFSNSPSLTVRPVEGNPAEALLDLADEEGFD
                     LIVVGNRGMAGASRLMLGNVPNKVSHHAPSSVLIVDTADGQEPGYRKMLAGTDGSATA
                     ARAVATAVELAEKTGAGLTIVTGAPNEQAGEQVLQPVRHEYPKVETVTLVGSPADAIV
                     ERAQKEGIDLIIVGNKGMTGARRFLLGSVPNKISHNAQTSVLIVNTTG"
     gene            3048..3740
                     /locus_tag="VEO00_03185"
     CDS             3048..3740
                     /locus_tag="VEO00_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYU57040.1"
                     /translation="MAEPVTGARLAEVPLFARLPPETREELATRFVSRAYPRGATVFQ
                     QGDPGRSLYVIDEGAVKISFLAADGREMVVTVLGPGDVFGEMALFDPEGARSADASAM
                     EAARLLSLSHEVFREYLVAHPSVSVELLGVLAKRLRESDRALQDAIFSDVPGRLARRF
                     LDLAERYGEARDGGTLIELPMTQEELARMVGSSRESVNKAIASFVHRGWLRTAGRRYV
                     VADPEALRRRAR"
     gene            complement(3737..4681)
                     /locus_tag="VEO00_03190"
     CDS             complement(3737..4681)
                     /locus_tag="VEO00_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYU57041.1"
                     /translation="MIRVVRVLAPNPGPFTLEGTNTWIVGENPALVVDPGPDDAGHLL
                     EVIDAAGPVAAILVTHDHPDHSPGAARLAEAAGAPVRAFRGVPPEQRLRDGEAIAAGR
                     VLLRAVHTPGHSRDHVAFFLQEDRALFTGDAVLGRGTSVIDPPEGDLAAYLRSLRAMQ
                     DLGARTIYPGHGPAVFSAAEKLAEYVDHRAERERQVLAGLRRGPATPEELVPAIYEGE
                     APPEVYPVAARSVLAHLIKLEREGRVEHVGPPAENRFAMAKARACEVCGRPALPRSRV
                     CERHSMALLQEELPSDAAGSDEASSVDKPAEGEQAGEP"
     gene            complement(4678..5271)
                     /locus_tag="VEO00_03195"
     CDS             complement(4678..5271)
                     /locus_tag="VEO00_03195"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYU57042.1"
                     /translation="MKPRPAATIVVARPGAAGPEVLVLERSGRSRFGPGFAVFPGGSL
                     EPGDDELARRWFGDPAEAYRACAVRELYEEAGILATAAGLCAEPSGAPIESIDFEPPP
                     PAALPEMARWIAPEGLPVRFDTRFYAIAAPPGLEPVPDGVEIERAWWADPAGVLDAAA
                     NGEASLMWPTLVTLRALRACRSVEDALALRVPQVAPA"
     gene            5424..6512
                     /locus_tag="VEO00_03200"
     CDS             5424..6512
                     /locus_tag="VEO00_03200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYU57043.1"
                     /translation="MSEVLAQALRERIAREGTITFAAFMEAALYDQDEGFYTRPPVGA
                     EGHFLTGPHVSPLFGRLLARQVAEAYDLLGAPDPFHVVEFGAGDGTLARQIIEGIAAA
                     PGLAAALRYVAVERSPGARAALADAGIKVAHEEALPDGLTGLVLANELLDNLPFHILV
                     REDGALRERFVALDGERFALVTATLSDPTLGPLAATLEEGAEGAVGAGPTRWLARAAG
                     MLARGYVLAIDYGGSGPRAPQGYRAHRTVEDLLADPGSADITAGVDLDALAGAARALG
                     LQVWGPVSQRAALLRLGFGTYAQAERDRTATERDAGDHLAAARRWARRSRESLLVDPA
                     ALGGLQVMCFGVNVATPLVAVRPADEVG"
     gene            complement(6509..7717)
                     /locus_tag="VEO00_03205"
     CDS             complement(6509..7717)
                     /locus_tag="VEO00_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HYU57044.1"
                     /translation="MADDTVVRPEERAAYTALSDQWLVRGGGVDIITVEPGLSTEEMV
                     LNIGPQHPSTHGVLRIVLALDGEVIVRAEPVIGYMHRSVEKLAEVRDVRQIGVLMNRH
                     DWLSSFNNELGFYLAVEKLCDIEVPERAQWIRTMFAEWNRVLNHLMFTGSFPLELGAM
                     TPMFYAFREREMIQALLESATGGRMHFMFCRAGGLKDDLPRGFLKQSAECLKWIRKKL
                     VDFEDLIMGNEIIHARTKGIGILSPELAVGYGASGPVLHASGVPMDPRKDDPYCKYGE
                     VEFDVPIGATGDAYDRLWVLVQRMWESCKIVEQCMDRLPPGPYVTPKVKPEPKLPEGE
                     VYQRTENPLGLMGYYLVGDGSDKPYRLKLRTASFSNVAVLPALLPSALVADLIAILGS
                     VFFVVGDVDR"
     gene            7745..8917
                     /locus_tag="VEO00_03210"
     CDS             7745..8917
                     /locus_tag="VEO00_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU57045.1"
                     /translation="MDFSVPDELRELTESFRAFLEREVRPVEERFTEALREESLPGDA
                     REEGLRLRRRSAELGFYAAHMPEEVGGGGLSNLGYTLLIEEGGKSGLRMAAFALGPPS
                     PEAPTPILMDLPEPVRDRVVLPLCRGETTMCFALTEPDAGSDAQSIRTRATLDGSEWV
                     LNGRKHFITNAPRADHALVFAVTDPQKRAAGGITAFFVDADTPGFVRGRHQKTIAGDT
                     GTGELIFEDCRVPAERVVGEVGFGFGSAMKFLNGGRAFIGALCLGIADHLIRVSTDYA
                     KERSTFGRPIGTNQAVQWMLADSALELHASRLMTYHLAWKVDRGEQPIMESSMVKLAN
                     TEMVNRVADRAVQIHGGMGLLAEGPVERLFRWVRMLRIVEGTSEIQRLIIARSLGL"
     gene            8926..>10065
                     /gene="selA"
                     /locus_tag="VEO00_03215"
     CDS             8926..>10065
                     /gene="selA"
                     /locus_tag="VEO00_03215"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HYU57046.1"
                     /translation="MGAGDVRRRIPSVDALLRAAAARRTARSFGRPVVRLALREVLDE
                     VRAGAKATPPQPVPPDEVILARAVGRAARTELGVRAAINATGVLLHTGLGRAPLGRDA
                     VRALGRVAGRYADVEVDRETGRREPRTAAAEYVLRALTGAEAALVVNNNAGALLLALA
                     ALARRREVVVSRGELIEIGGDFRLPDIMGASGAKLVEVGTTNRTSPTDYERAIGERTA
                     LALKVHPSNYRVTGFTRSVEVGALAALAHARGVPVLHDLGSGLLTRLAGVPPDEPAAA
                     ESLAAGADLVCFSGDKLLGGPQAGILLGRSEMIEKLRRHPLARALRVDVLAVAALQAT
                     LRAYATDGLDRVPFWRAARASRAEIRRRAAALAGRIGGATVVPTEA"
BASE COUNT         1311 a         3727 c         3684 g         1343 t
ORIGIN      
        1 gaccttcgcc tcctgtcgat gggcccgcgc accgggctcg ccgagatccg cctgcccgag
       61 gtgcagcccg gctcctcgat catgccgggg aaggtcaacc cctcggtccc cgagatggtg
      121 aacatggtct gcttccgggt ctcgggttcg gagcacacgg tggcgctcgc ggttggcgcc
      181 ggccagctcg acatgagcgt gatgacgccg gtgatcgccg acgagttgct ggaggcgcag
      241 accctgctcg ccgccgcatg ccggacgctc gcggagcgct gcgtggcggg gatcgaggcc
      301 gacattgagc gctgccgggc ctacgcggag gcctcgctcg ggctggcgac ggccctgcgc
      361 ccggctctcg ggtatgcacg tgccgccgac ctggcggtgg aggcgatgcg cagcgggcgc
      421 accctgcgcg agatcgtctc cgagcgggga gcgctccccc acgagaagct ggacgaggtc
      481 ctcgacccgg cacgctacgc ctgagcggcg cgccggcgaa ggtcctcccg gaaggtccgg
      541 taggcggccg cggggtccca accaccgcgc cagccctcgg gcgccagccg gcccttcagg
      601 ccctcttccg ccgcccggac ggccacggcc gcgcggaacc actcctgccc gccgggctgc
      661 agcacgctct ccccggaggc ccccgccggc ctgagcgcgg gatcgtcgac cacgatcgcc
      721 gccggacgga agacccgctg caacgcggcg cgggcctcct cgtcgggggc ggaagcgatc
      781 gtcacggccc ggccgtcgaa ggcgacctcg cccaccgtcc ggttcggatc gtcggcgggc
      841 gtgaacacgg cgcgcatcac gggccgctca ccctcgcgat cccttcgggg agccggcggc
      901 gacccacagg taggcgacga gcgcgagcgt cccgggcatg gccgcatcgt agagcgccag
      961 cgcatccccg accgccgccc ggtcctcctc gctgaacccg cggtcgcgca gcgcgcccca
     1021 ctgcagcgag atcacgtggg gcagccggcg gacgccctcg acgatcgctc gccggatccg
     1081 gccggaggcc ttcgagaacg tgtcgctcgc cgtgaagcgg cgaccggcgc gccaggcacg
     1141 tgtcaggtac ccgggccagg ggcgcagggc gagcagggcg tcggggggcc gcggcgcgcc
     1201 gagccgcttc gccgccgccg ccagggcctc ggcggcctcg tccccctcac cgggcaactc
     1261 caggcgagcg tgcggctgcc acgccggaac cccgcgcttc ggcggcggct cctcgatccc
     1321 gctcccgccg gccgggcggc cgcgagcggc gcgcgccagc gcgtgcacga cgagggcggc
     1381 cttcgcctgg ttctcgtgga acgcccgcag cacgcgcgcc acctccgcca cctcgtccgc
     1441 ctggagcctc gccaggcgga tccactcggc gtgatcgggc ggcgcgccgg cctccgccgc
     1501 agcggccgcc aggtcgcgga cgaccttcgc ggacgccagg aaggtcgggc ccacgttcgg
     1561 gcggatcgcc gaccaggcgg cggtgaagaa catcgggtac tgggccgcct cggcgatcgc
     1621 ggagtcgatc cagcgcatgc cgagcgtctc ccggacgtcc accaggacgg cggggaccag
     1681 gctcccgacc ggcggtccgc cctccgctgg ggcgttcggc gtggacgaga ccgatgcgtt
     1741 cagcccgtga cgctcttctc ggtctggcga aggcgcaggg cgagctcctt cagaatcttc
     1801 gggatcagcg ccggctcctt ctccaggagg gaccggaagt cccaccggag gatcgacagg
     1861 cagcgcatcg gcgtcaccgc cgtcacggtg gccgtgcgcg ggccgccgtc gagcagcgac
     1921 acctccccga agaacgaacc ggggagcagc cgggccaccg acttcccgcg acgggtcacc
     1981 ttcgcctcgc cctccagcac cacgtagaag gacaggccat cctcgccctc ctcggcgatc
     2041 acgtcgcccg cctcgaacgt cacctcgctg gccagcccgg caacgcgctt caggtcgcgg
     2101 cgcgagcacc gggagaacag cgggaccgac gcgagttgcg cctcgccgcc catcagcccg
     2161 tggtgttcac gatcagaacg ctcgtctgag cgttgtgcga gatcttgttc gggacgcttc
     2221 ccagcaggaa gcggcgggcc ccggtcatgc ccttgttgcc cacgatgatc aggtcgatcc
     2281 cctccttctg cgcgcgctcg acgatcgcgt ccgccgggct cccgacgagc gtgaccgtct
     2341 cgaccttcgg atactcatgg cggaccggct ggagcacctg ctcgccggcc tgctcgttcg
     2401 gcgcgccggt gacgatggtc aggccggcgc cggtcttctc cgccagctcc accgcggtgg
     2461 cgaccgcccg ggcggccgtt gccgagccgt cggtccccgc cagcatcttg cggtaccccg
     2521 gctcctgccc gtccgcagtg tccacgatca ggacggagct gggggcgtgg tgcgacacct
     2581 tgttcgggac gttcccgagc atcaggcggc tggccccggc catcccgcgg ttcccgacga
     2641 cgatcaggtc gaagccctcc tcgtcggcca ggtcgagcag ggcctcggcc gggttgccct
     2701 cgacggggcg gacggtgagg ctcggcgagt tggagaatct gttctggacg tcgcggagga
     2761 tggcccgcgc gatgtcggcc gtggggtcgc cctgccgggc ggaaaggatc tccggggcgt
     2821 ccacctgcgg cgtggcatag accgagatcg ccgtcacctc cgcgccgatg gcctccgcta
     2881 gcttcagggc atgcgccacg gccatcgtgg ccgtgtcgga cccatcggtc cccacgagga
     2941 tcttgcggaa cacgtcccct cccgcgtcgc ggccgcgggc cggccgcccg gcggagcgta
     3001 gcaggccgtg cggagagctg tgaagggacg cgtacacttc cgccgttatg gccgaaccgg
     3061 tcaccggtgc ccgcctggcg gaggtcccgc tgttcgcccg gctcccgccg gagacccggg
     3121 aggagctggc gacccgcttc gtcagccgcg cctacccgcg cggggcgacg gtcttccagc
     3181 aaggcgaccc cgggcgctcg ctgtacgtga tcgacgaggg cgcggtgaag atcagcttcc
     3241 tggccgcgga cggccgtgag atggtcgtca ccgtgctcgg accgggcgac gtcttcggcg
     3301 agatggccct gttcgacccg gagggggcgc gctcggcgga cgcctcggcc atggaggccg
     3361 cccggctcct gtcgctctcg cacgaggtgt tccgcgagta cctggtggct cacccgagcg
     3421 tgtcggtgga gctgctcggc gtgctggcca agcgcctgcg ggagtccgac cgggcgctgc
     3481 aggacgccat cttctccgac gtccccgggc gcctggcccg gcggttcctg gacctggccg
     3541 agcgctacgg cgaggcccgc gacgggggca cgctgatcga actgccgatg acccaggagg
     3601 agctcgcccg gatggtcggg tcgtcgcggg agagcgtgaa caaggccatc gcctcgttcg
     3661 tccatcgcgg ctggttgcgc acggcgggcc ggcgctacgt ggtggccgac cccgaggccc
     3721 tccgccgccg ggcccgctag ggttccccgg cctgctcccc ctctgccggc ttgtcaacgc
     3781 tcgatgcctc gtcggacccg gccgcgtccg acggcagctc ctcctgcagc agcgccatcg
     3841 agtggcgctc gcagacgcgg ctgcgcggga gggccggacg gccgcacacc tcgcaggccc
     3901 gcgccttcgc catggcgaag cggttctccg ccggcggccc gacgtgctcg acccggccct
     3961 cccgttccag cttgatcagg tgcgcgagca ccgagcgcgc cgcgaccggg tagacctccg
     4021 ggggggcctc gccctcgtag atggcgggga cgagctcctc cggggtggcc gggccacgcc
     4081 gcaggccggc caggacctgc cgctcgcgct cagcgcgatg gtcgacgtac tcggcgagct
     4141 tctcggcggc cgagaacacg gccggcccgt ggccagggta gatcgtgcgc gctccgaggt
     4201 cctgcatcgc ccgcagcgaa cgcaggtacg cggccaggtc gccctccggc ggatcgatca
     4261 cgctcgtccc gcgcccgagc acggcgtcgc cggtgaacag cgcgcggtcc tcctgcaaga
     4321 agaacgccac atggtcgcgg ctgtgccccg gcgtgtgcac cgctcggagc aggacccgcc
     4381 cggcggcgat cgcctcgccg tcgcgcaagc gctgctcggg cggtacgccg cggaacgcgc
     4441 ggaccggcgc gccggccgcc tcggccaggc gcgccgcgcc cggcgagtgg tcggggtgat
     4501 cgtgggtgac gaggatggcg gccaccgggc cggccgcgtc gatgacctcg agcaggtggc
     4561 cggcgtcgtc ggggccgggg tcgacgacga gcgccgggtt ctcgccgacg atccatgtat
     4621 tcgtcccctc cagggtgaac gggccggggt tcggcgccag gacgcgcacg acgcggatca
     4681 cgccggcgcc acctgcggga cgcgcagcgc cagggcgtcc tcgaccgagc ggcacgcgcg
     4741 cagcgcccgc agcgtgacga gcgtcggcca catcagcgat gcctcgccgt tcgccgccgc
     4801 gtcgagcaca ccggccgggt cggcccacca ggcccgctcg atctccacac cgtcgggcac
     4861 cggctcgagc cccggcggcg cggcgatcgc gtagaagcgc gtgtcgaacc ggaccgggag
     4921 gccctccggc gcgatccagc gcgccatctc gggcaacgcc gcgggcggcg gcggctcgaa
     4981 gtcgatcgac tcgatcggag cgccggatgg ctcggcgcac aatccggcgg cggtcgccag
     5041 gatgccggcc tcctcgtaca gctcgcgcac ggcgcaggcg cggtaggcct ccgcgggatc
     5101 gccgaaccag cgccgcgcca gctcgtcgtc cccgggctcg agcgagccgc cggggaacac
     5161 cgcgaacccc ggcccgaacc ggctccggcc ggaccgttcg agcaccagca cctcggggcc
     5221 agcggcgccc gggcgcgcca cgacgatcgt cgccgcgggc cgcggcttca tccggccttg
     5281 tcctttgggg gttcgctcgc cgggcgtggt cgcgtggatc gacgcccggg gcgagctacc
     5341 cccccaaacc cccccggctc catgcgggcg attgtgccag acgcaaccga acggcctcgc
     5401 cgaagcgccc ataggatcgt cgcgtgagcg aggttctggc gcaggccctg cgcgagcgga
     5461 tcgcgcgcga gggcacgatc accttcgcgg cgttcatgga ggccgcgctg tacgaccagg
     5521 acgaggggtt ctacacccgc ccaccggtcg gcgccgaggg ccacttcctg accggcccgc
     5581 acgtgtcgcc gctgttcggg cggctgctgg cgcggcaggt cgcggaggcc tacgacctgc
     5641 tcggcgcgcc ggacccgttc cacgtggtcg agttcggcgc cggcgacggg acgctggccc
     5701 ggcagatcat cgaggggatc gccgccgcgc cgggactggc cgcggcgctc cgctacgtgg
     5761 cggtggaacg ctcacccggg gcgcgggctg ccctggccga cgcggggatc aaggtggcac
     5821 acgaggaagc gctcccggac ggcctgaccg gcctggtcct cgccaacgag ctgctggaca
     5881 acctgccgtt ccacatcctg gtgcgtgagg acggcgcgct gcgcgagcgg ttcgtcgcac
     5941 tggacggcga gcggttcgcc ctggtcaccg ccacgctctc ggaccccacg ctcgggccgc
     6001 tcgcggccac gctggaggag ggcgccgagg gcgcggtcgg cgccgggccg acacggtggc
     6061 tggcccgggc ggccgggatg ctcgcgcgag gctacgtact ggccatcgac tacgggggca
     6121 gcggaccgcg tgccccccag ggctaccgcg cccaccggac ggtcgaggac ctgctggccg
     6181 acccgggctc ggccgacatc accgccggcg tggacctcga cgccctggcc ggcgccgcgc
     6241 gagcgctcgg cctgcaggtc tggggaccgg tcagccagcg cgccgccctg ctccgcctgg
     6301 gtttcgggac ctatgcccag gccgaacgcg accggaccgc gaccgagcgc gacgccggcg
     6361 accacctcgc cgccgcccgc cgctgggccc gccgcagccg cgagtcgctc ctggtcgacc
     6421 ccgccgccct cggcggcctg caggtgatgt gcttcggcgt gaacgtggcg acaccgctcg
     6481 tcgcggttcg gcctgccgat gaggtcggtt agcggtcgac gtcgccgacg acgaagaaga
     6541 cgctgccgag gatggcgatc aggtcggcga ccagggcgct ggggaggagc gccgggagca
     6601 ccgccacgtt cgagaacgac gccgtgcgga gcttcaggcg gtagggcttg tccgagccgt
     6661 cgccgaccag gtagtagccc atcagcccga gcgggttctc ggtgcgctgg tacacctcgc
     6721 cctccggcag cttcggctcg ggcttgacct tcggcgtgac gtacggtccc ggcggcagcc
     6781 ggtccatgca ctgctcgacg atcttgcacg actcccacat ccgctggacc aggacccaca
     6841 gccggtcgta cgcatcgccc gtcgcgccga tcggcacgtc gaactccacc tcgccgtact
     6901 tgcagtaggg atcgtccttg cgagggtcca tcggcacgcc cgacgcgtgc agcacgggcc
     6961 cggaagcccc gtatccgacg gccagctcag gagagaggat cccgatgccc ttcgtccggg
     7021 cgtggatgat ctcgttcccc atgatcaggt cctcgaagtc gacgagcttc ttgcggatcc
     7081 acttcaggca ttccgccgac tgcttcagga acccgcgcgg caggtcgtcc ttcaggcccc
     7141 cggcgcggca gaacatgaag tgcatccggc cgccggtggc cgactcgagc agcgcctgga
     7201 tcatctcccg ctcgcggaag gcgtagaaca tcggcgtcat cgccccgagc tccagcggga
     7261 acgacccggt gaacatcagg tggttcagca cgcggttcca ctcggcgaac atcgtccgga
     7321 tccactgcgc tcgctcggga acctcgatgt cgcagagctt ctcgacggcc aggtagaacc
     7381 ccagctcgtt gttgaacgac gacagccagt cgtggcggtt catcagcacc ccgatctggc
     7441 gcacgtcgcg cacctcggcg agcttctcca ccgaccggtg catgtagccg atcaccggct
     7501 ccgcccggac gatcacctct ccgtcgagcg cgagcacgat gcgcagcacg ccatgggtcg
     7561 aggggtgctg cgggccgatg ttcaggacca tctcctcggt ggagaggccc ggctcgacgg
     7621 tgatgatgtc cacgccgccg ccgcgaacca gccactggtc gctgagggcg gtgtacgcgg
     7681 cgcgctcctc cgggcgaacg accgtgtcgt ctgccatcga gctcagtgta tacagttcgc
     7741 ccgcatggac ttctccgtcc ccgacgagtt gcgggagctc accgagtcgt tccgcgcgtt
     7801 cctggaacgc gaggtgcgcc cggtcgagga gcgcttcacc gaggccctgc gcgaggagtc
     7861 gctgccgggg gacgcgcgcg aggaggggct gcggctgcgg cgccgttcgg ccgagctcgg
     7921 gttctacgcc gcgcacatgc ccgaggaggt cgggggcggc gggctctcga acctcggcta
     7981 cacgctgctc atcgaggagg gtgggaagtc cggcttgcgg atggcggcgt tcgcgctcgg
     8041 gccaccgtcc ccggaggcgc cgaccccgat cctgatggac ctgccggagc ccgtccgcga
     8101 cagggtcgtc ctcccgctct gccgcggcga gacgacgatg tgcttcgcgc tgaccgagcc
     8161 cgacgccggc tcggacgcgc agtcgatccg cacccgggcc acgctcgacg ggagcgaatg
     8221 ggtcctgaac gggcgcaagc acttcatcac gaacgccccg cgggccgacc acgcgctcgt
     8281 cttcgcggtc accgatccac agaagcgcgc cgccggcggg atcaccgcgt tcttcgtcga
     8341 cgccgacacg cccgggttcg tgcgcggcag gcaccagaag acgatcgccg gcgacaccgg
     8401 gaccggggag ctgatcttcg aggactgccg ggtaccggcc gagcgcgtgg tcggtgaggt
     8461 cgggttcggt ttcggctcgg cgatgaagtt cctgaacggc ggccgggcct tcatcggcgc
     8521 gctgtgcctc gggatcgccg accacctgat ccgggtctcg acggactacg cgaaggagcg
     8581 ttcgacgttc ggcaggccga tcggcacgaa ccaggccgtg cagtggatgc tggccgacag
     8641 cgcgctcgag ctccacgcat cgcggctgat gacgtaccac ctggcctgga aggtcgaccg
     8701 cggcgagcag ccgatcatgg agtcatcgat ggtgaagctc gcgaataccg agatggtcaa
     8761 ccgggtcgcc gaccgcgcgg tgcagatcca cggcgggatg ggcctgctgg ccgagggacc
     8821 ggtcgagcgg ctgttccggt gggtgcggat gctgcggatc gtggagggca cgagcgagat
     8881 ccagcgcctg atcatcgccc gcagcctcgg cctgtagagg tgccggtggg cgcgggggac
     8941 gtccggcggc ggatcccctc cgtggacgcc ctgctgcgtg cggcggccgc tcgccggacg
     9001 gcgcgctcgt tcggtcgccc cgtcgtccgg ctggcgcttc gagaggtcct cgacgaggtc
     9061 cgggccggtg cgaaggcgac cccaccgcag ccggtcccgc cggacgaggt catcctggcg
     9121 cgggccgtgg gccgggcggc gcgaacggag ctcggggtgc gggcggcgat caacgccacc
     9181 ggagtgctac tccacacggg cctcgggcgc gccccgctcg gccgggacgc cgtgcgcgcg
     9241 ctcgggcgcg tcgcgggacg ttacgccgac gtggaggtgg atcgcgagac cggccgccgc
     9301 gagccgcgga ccgcggccgc cgaatacgtg ctgcgggccc tcaccggggc ggaggccgcc
     9361 ctggtcgtca acaacaacgc cggggcgctg ctgctggcgc tggccgcgct ggcgcggcgg
     9421 cgcgaggtgg tcgtctcgcg cggcgagttg atcgagatcg gcggggactt ccgcctcccc
     9481 gacatcatgg gcgccagcgg cgcgaagctc gtcgaggtcg gcacgacgaa tcggacgtcg
     9541 ccaaccgact acgagcgcgc gatcggggag cgcaccgcgc tcgcgctgaa ggtgcacccg
     9601 agcaactacc gcgtcaccgg gttcacgcgg tcggtcgagg tgggcgcgct cgccgcgctg
     9661 gcgcacgccc ggggcgtccc cgtgctgcac gatctcggga gcggcctgct gacgcgcctc
     9721 gccggcgtgc cgcccgacga gcccgccgcc gccgagtcgc tcgccgccgg cgcggacctg
     9781 gtctgcttca gcggggacaa gctgctcggc ggcccgcagg ccgggatcct cctcggacgg
     9841 tccgagatga tcgagaagct gcgccgccac ccgctcgccc gggcgctgcg cgtggacgtc
     9901 ctggcggtgg cggccctcca ggcgacgctc cgcgcgtacg ccaccgacgg gctggaccgg
     9961 gtgccgttct ggcgggccgc ccgggcctcg cgggccgaga tccggcgccg ggcggcggcg
    10021 ctcgccggtc ggatcggggg ggccaccgtg gtgcccaccg aggcg
//
LOCUS       DASPWO010000138         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_700761, whole genome shotgun sequence.
ACCESSION   DASPWO010000138 DASPWO010000000
VERSION     DASPWO010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700761"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="VEO00_03220"
     CDS             complement(<1..1514)
                     /locus_tag="VEO00_03220"
                     /inference="COORDINATES: protein motif:HMM:NF015118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HYU57047.1"
                     /translation="MSAFFLEGDGARRRGWSQILDIFRKTVTDIEDALSARLTLVRLD
                     SAVLLTYLHACITGLRHPVRVPAIPMYLDAVPATQDLVGGFRPRIGERHLRVIAVMGF
                     PMDSVPEILGFLNRLPVEYRWSTRFIFLDPASAERALRVYRRNWFQKRHGLLGLLKES
                     FNFGTVTFANKDAVAMAQDADDAVGEASANAVRFGYFTCNVLLFDPDRERLDGTAREV
                     VKQLQHRGFGARAEEVNALEAYLGSLPGHGYQNVRKPLLHTLNLADLLPLTSIWPGLE
                     QHPCPFYPPGSPSLCYAATAGATPFRLNLHVSDVGHTLILGPTGSGKSTLVGLLIAQV
                     FRYPGAQVVLFDKDYSAYVLAHACGAEYYDIAGEGERGLAFTPLAAIDRDHERRWAQE
                     WIEVLLDLQGVTPTPAQRAALWRALELLAESPSRTMTDLVHTLQDEGLREALAPYTLS
                     GPLRHLLDAQTEAVGEGRLQVFEMSHLMALGQKHLVPILLYLFHRVEQRLETGTP"
     gene            complement(1547..1735)
                     /locus_tag="VEO00_03225"
     CDS             complement(1547..1735)
                     /locus_tag="VEO00_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57048.1"
                     /translation="MNAALVRLGNGWMLHVDAIRRPSVGYPDGGAFPDATTRLIDDER
                     RERYTAEAPTSRAPITLP"
     gene            complement(1732..2226)
                     /locus_tag="VEO00_03230"
     CDS             complement(1732..2226)
                     /locus_tag="VEO00_03230"
                     /inference="COORDINATES: protein motif:HMM:NF016957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HYU57049.1"
                     /translation="MDEPSRAPIHPSLTRPLLLGGAERELVLVNGTIIAALIFGVGFH
                     WTSLTIAGLLATAGHWALTRAARYDPRLSRIYVRHVRYQPYYPRGRQARRGRPTRDRG
                     RCRSRPPGSLRPSGGTRPRRRGPRSSRGRRDLSASASRPPPSVLHGRVDPGGPRLGQG
                     PRAR"
     gene            complement(2226..2549)
                     /locus_tag="VEO00_03235"
     CDS             complement(2226..2549)
                     /locus_tag="VEO00_03235"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HYU57050.1"
                     /translation="MPDHRKLRTLVFPGCLVLLGSLRSLAEAAATGPAMPWDTPLNTI
                     LNSLSGTVAHVRITVAVIVTGLVFAFTEHGSGARRLFGVAFGGALALGALSFMTAVGW
                     SGATF"
     gene            complement(2542..3531)
                     /gene="trbB"
                     /locus_tag="VEO00_03240"
     CDS             complement(2542..3531)
                     /gene="trbB"
                     /locus_tag="VEO00_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HYU57051.1"
                     /translation="MRAMHPEQQQRLYAKLCRELGPATVAALADPDVTEVMCNPDGAL
                     WIESHGQGMREIGDRLTETQVESLIGTGAALLGTVVHGRAPIVEGELPTNGYRFEGIL
                     PPVSTRPTFVIRKRASQVYTLDEYVNAKILSAAQQRVLLEAVRDRWNIVIAGGTASGK
                     TTLGNALIREIVALGDPTERIVILEDTRELQCEARNAVQLHTGDVADLTRLVRVTMRL
                     RPDRIIVGEVRGGEALALLKAWNTGHPGGVTTVHANSAAAALLRLDSLIQESGVPPQP
                     RLVAETVDLVVFIARTPEGRRVRELALVNGYDVVHGSYLLEPVCPTPTEAEHA"
BASE COUNT          545 a         1257 c         1281 g          542 t
ORIGIN      
        1 ggagtccccg tctccagccg ctgctccact cgatgaaaga ggtagagcag gatcgggacg
       61 aggtgtttct ggccgagcgc catcaggtgg gacatctcga agacctggag ccggccctca
      121 cccacggcct cggtctgggc atcgagcagg tgcctgagcg ggccgctcag ggtgtagggg
      181 gccagcgcct cccggagccc ctcgtcctgc agcgtgtgga ccaggtcggt catcgtgcgg
      241 ctcgggctct cggccaggag ctcgagggcg cgccagagcg ccgcgcgctg cgcgggcgtc
      301 ggcgtgacgc cctgcaggtc gagcaggacc tcgatccact cctgggccca tcggcgctcg
      361 tgatcccggt cgatcgcggc gagcggcgtg aaggccagcc cgcgctcgcc ctctcccgcg
      421 atgtcgtagt actcggcgcc gcaggcgtgc gcgagcacgt acgccgagta gtccttgtcg
      481 aacagaacaa cctgagcgcc ggggtaccgg aagacctgcg cgatcagcag gccgaccagc
      541 gtcgacttcc cggacccggt gggcccgagg atcagggtgt ggccgacgtc gctcacgtgg
      601 aggttcaggc ggaacggtgt ggccccggcg gtggccgcgt agcagagcga gggactcccg
      661 ggcggataga aggggcacgg gtgctgctcg agccccggcc agatgctggt gagcggcaac
      721 agatcggcca ggttcagggt gtgcaggagc ggcttgcgga cgttctggta gccgtgcccg
      781 ggaagggatc ccaggtaggc ctcgagggca ttcacctctt ccgcccgcgc gccgaagccg
      841 cgatgctgga gctgcttgac cacctcgcgc gccgtgccat ccagccgttc gcggtcggga
      901 tcgaacagca acacgttgca cgtgaagtag ccgaagcgca ccgcgttggc gctggcctcg
      961 cccacagcgt cgtccgcgtc ctgggccatg gccaccgcgt ccttgttcgc gaaggtgacc
     1021 gtcccgaagt tgaacgactc cttgaggagc ccaagcagcc cgtgtcgctt ctggaaccag
     1081 ttcctccggt agacccggag cgcgcgctcg gcgctggccg ggtccaggaa gatgaagcgc
     1141 gtcgaccagc gatactccac cggcaggcgg ttcaggaacc ccaggatctc cggcaccgag
     1201 tccatcggga atcccatgac ggcgatgacg cgaagatggc gctcgccgat cctcggccga
     1261 aagccgccga ccaggtcctg ggtcgcgggc acggcatcga ggtacatcgg aatcgcgggc
     1321 acccgcaccg ggtgtcgcag gccggtgatg cacgcgtgca ggtacgtgag gagcacggcg
     1381 gagtcgaggc gcacgagcgt cagacgcgcc gagagcgcat cctcgatatc cgtcaccgtc
     1441 ttcctgaaga tgtcgaggat ctggctccag ccccggcgcc gcgcgccgtc cccttcgagg
     1501 aagaacgccg acacgcggtg ctcgatctcc gcgggtgggc ggtaggtcag ggcaaggtaa
     1561 taggcgctct cgaagtgggt gcctccgcgg tgtagcgctc gcggcgctcg tcgtcgatga
     1621 gtcgcgtggt cgcatcgggg aaggcgccgc cgtcggggta gccgacgctc ggccggcgga
     1681 tcgcgtcgac gtggagcatc cagccgttcc cgaggcgcac cagggccgcg ttcacctggc
     1741 tcgcggcccc tgaccaaggc gcggcccgcc tgggtctacc cgtccgtgga ggactgatgg
     1801 tggcggcctt gacgccgaag ccgaaaggtc tcgccgacct cgtgagctac gcggccctcg
     1861 tcgacgaggg cgtgtgcctc ctgaaggacg gaggcttcct ggcggcctgg acctacaccg
     1921 gccccgatct cgagtcggcc ggccacgacg agcttgccgt cctctcgggt agtacggctg
     1981 gtaccggacg tgacggacgt agatccggct cagccgcggg tcgtacctgg ccgcccgtgt
     2041 cagcgcccag tgcccggccg tggcgagcag gccggcgatc gtgagactcg tccagtggaa
     2101 tcccacgccg aaaatcagcg cggcgatgat ggtgccgttc accagcacga gctcgcgttc
     2161 ggcaccgccc agcaggagcg gtcgagtgag tgacggatgg atcggcgctc ggctgggctc
     2221 gtccatcaga aggtggctcc cgaccagccg accgcggtca tgaagctcag ggccccgagg
     2281 gcgagcgccc cgccgaaggc gaccccgaac aggcgccgcg ccccggaccc gtgctccgtg
     2341 aacgcgaaga ccagaccggt cacgatcacg gccacggtga tgcgcacgtg ggccaccgtg
     2401 ccgctcaggc tgttcaggat ggtgttcagg ggcgtgtccc agggcatcgc cggccccgtg
     2461 gcggccgcct cggccagcga tcgcagagag ccgagcagca ccaggcagcc cgggaagacg
     2521 agcgtgcgga gctttcgatg gtcaggcatg ttcggcctcc gtcggtgtcg ggcagactgg
     2581 ctccaggagg tagcttccgt gcaccacgtc gtagccattc acgagcgcga gctcgcgcac
     2641 gcggcgcccc tccggggtgc gagcgatgaa cacgaccagg tccaccgtct cggccaccag
     2701 gcgtggctgc ggtggcacgc ccgactcctg gataagactg tccagacgta gcagggccgc
     2761 ggccgcgctg ttggcgtgca ccgtcgtgac cccgccggga tgtccggtgt tccaggcctt
     2821 gagcagcgcc agggcctctc cgccccgcac ctctcccacg atgatgcgat ccgggcggag
     2881 acgcatcgtg acccggacca gccgggtcag gtccgccacg tcccccgtat ggagctggac
     2941 cgcgttccgc gcctcgcact ggagctcgcg cgtgtcctcg aggatcacga tccgctccgt
     3001 cgggtcgccg agcgcgacga tctcgcgtat cagggcgttc cccaacgtgg tcttacccga
     3061 tgccgtcccg ccggcgatga cgatgttcca ccggtcgcgg acggcctcga ggagcacccg
     3121 ctgctgcgcc gcggacagga tcttggcatt gacgtactcg tccagggtgt agacctggct
     3181 ggcgcgcttc cggatgacga aggtgggccg cgtcgagacc ggcggcagga tgccctcgaa
     3241 gcggtacccg ttggtcggca gctccccctc gacgatcggc gcgcggccgt gcaccaccgt
     3301 gccgaggagc gcggcccccg tgccgatgag gctctcgacc tgggtctccg tgagacggtc
     3361 gccgatctcc cgcatgccct gcccgtgcga ctcgatccag agggccccgt ccgggttgca
     3421 catcacctcg gtcacatcag ggtcggccag cgccgccacc gtcgcggggc cgagctcgcg
     3481 gcagagcttg gcgtagagcc gctgctgctg ttccgggtgc atcgccctca tgcgtcgcct
     3541 ccgccgtcga gactcagctg caccgccgcc tggccgttca cgccccccga gcgcacgagg
     3601 cgctcgacgg tccttcgcca gttcc
//
LOCUS       DASPWO010000139         3760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_363352, whole genome shotgun sequence.
ACCESSION   DASPWO010000139 DASPWO010000000
VERSION     DASPWO010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363352"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VEO00_03245"
     CDS             <1..232
                     /locus_tag="VEO00_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HYU57052.1"
                     /translation="VGLADVVLQSVQTALEPEAKSSMCRDVERGRPLEVEAINGAVVR
                     YGEEAGVPTPRNRTILDALLPLHRAALVTRSS"
     gene            276..632
                     /locus_tag="VEO00_03250"
     CDS             276..632
                     /locus_tag="VEO00_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57053.1"
                     /translation="MRVVPAAFLMLLGALSLVAPAQWWRRRWSTTGIWIARTVSPHLL
                     VAAPALGLFLIALGLTVLWSPGVIFAALAGLLFVGVILASGRRRPVVAHDLREPGERA
                     GPADERFPPQSERRTG"
     gene            complement(629..1936)
                     /gene="pfp"
                     /locus_tag="VEO00_03255"
     CDS             complement(629..1936)
                     /gene="pfp"
                     /locus_tag="VEO00_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HYU57054.1"
                     /translation="MVDGRHRLGILCGGGPAPGINSVISAATIEAINSGWDVIGIRDG
                     FSHLILGSTDQVQRLTITDVSRIHVLGGSLLFTSRANPTVRDEGAADPEWRMHNCVKA
                     LSELGVDALVTIGGDDTAFSASKVAEAAGGSIKVAHVPKTIDNDLPLPRGISTFGFQT
                     ARHVGVELVNNLMTDAMTTKRWFFVLAMGRSAGHLAVRIGKAAGATLTVIAEEFPPDR
                     PIRLDHLVDLLEAAILKRFAHGRPFGVVILSEGIALRLDEADLKKAMPDVEIDEHGHI
                     RLGELNLDGVLADMVKQRFRSRGQKVTITAKNIGYELRCAPPIPFDIEYTRDLGYGAV
                     DYLRRLFRDGSDEIGAMITIQEEEMVPLPFGSFTDPATGRPQIREVNLDSGMYRTATE
                     YMIRLEREDLEDPEKLAAIARVAGMTPEGFADRYGYLVGGRGG"
     gene            2038..3099
                     /locus_tag="VEO00_03260"
     CDS             2038..3099
                     /locus_tag="VEO00_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HYU57055.1"
                     /translation="MRTKSLIAACAMLAILAAGLAPGASAEQTIERRLSEARTSRDRA
                     NRSVRDAEARLAALTAEYERVRSDLDLAVRDMVVAQLAQRGATDDLAAAQDALDRKAA
                     AAYEAGPTATLELIFGSASTADLAAAEVFAASAFSVGQTQVDEVVRLRASLEGVALRL
                     ERNRADVAAALRDLDRDTSAVTAEVERARAVAADAGLEVTKLEKEQRELDRARRRLEA
                     SLAPLIDATRGRDQSELLARLGPSQGRGCDIPSGLTPTGDTLAGDSSWYGWDFAGNPT
                     ASGAIYDPRLFTAANKDLPLNVFLRIHFRDECAIVLVNDRGPYVGDRIIDVSQAVAEY
                     LGYKSAGVAWVTADVLVSK"
     gene            3253..>3760
                     /locus_tag="VEO00_03265"
     CDS             3253..>3760
                     /locus_tag="VEO00_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HYU57056.1"
                     /translation="MPSVLAVDSGTTGVTALVVDEDGAVRGRGYREFPQSFPRPGWVE
                     HDPDHWWEAMLAACSQALGGAGLDADDLAALGITNQRETTVVWERDALRPIHPAIVWQ
                     DRRTASLCQQLREEGWEDRVRDRTGLVIDPYFSGTKLAWLLDNVPQAREDAEAGRLAF
                     GTVDSYLLA"
BASE COUNT          544 a         1335 c         1318 g          563 t
ORIGIN      
        1 cgtcgggctc gccgacgtcg tcctccagag cgtccagacg gccctcgagc ccgaggcgaa
       61 gtcttcgatg tgccgggacg tcgaacgcgg caggcccctg gaggtcgagg cgatcaacgg
      121 ggccgtcgtc cggtacggcg aggaggcggg cgttcccacg ccgcgcaacc gaacgatcct
      181 ggacgccctg ctcccactcc accgggccgc gttggtcacc cgctcgtcct gagcccgttc
      241 ggcatagtcg acccgagatc gggtatgcgc ttcgtgtgag agtcgtgccc gcggcattcc
      301 tgatgctgct cggggccctg tcgctcgtcg cgcccgccca atggtggcgc cggcgatgga
      361 gcaccaccgg gatctggatc gcccggacgg tgtcgcctca tctgctggtg gccgcgccgg
      421 ccctgggtct attcctgatc gcgctgggcc tcacggtcct gtggtcgccg ggggtcatct
      481 tcgccgcgct ggcgggtctg ctgttcgtcg gcgtgatcct ggcgtccgga cggcgccgcc
      541 cggtggtggc gcacgacctg cgcgaaccag gcgaacgtgc cgggcccgcc gacgagcggt
      601 tccctcccca gtcagagcgc cgaaccggct agccgcccct tccccccacc agatacccgt
      661 agcgatcggc gaatccttcg ggcgtcatcc cggcgacccg cgcgatggcg gcaagcttct
      721 ccggatcctc cagatcctcg cgctcgagcc ggatcatgta ctcggtggcc gttcggtaca
      781 tcccgctgtc gagattcacc tcgcggatct gcggcctccc cgtggcggga tccgtgaacg
      841 atccgaacgg gaggggcacc atctcctcct cctggatcgt gatcatggcg ccgatctcgt
      901 cgctcccatc ccggaacagc cttcgaaggt agtcgacggc cccgtagccc aaatcgcggg
      961 tgtactcgat gtcgaagggg atcggggggg cgcaccggag ctcgtagccg atgttcttcg
     1021 cggtgatcgt gaccttctgt ccccgggatc ggaaccgctg cttgaccatg tcggccagca
     1081 cgccgtccag gttcagctca cccagtcgga tgtgcccgtg ctcgtcgatc tcgacgtcgg
     1141 gcatggcctt cttcaggtcg gcctcgtcca gccgcagggc gatcccctcg gacaggatca
     1201 ccacgccgaa gggccggccg tgcgcgaagc gcttcaggat ggcggcctcc agcaggtcga
     1261 ccaggtggtc gagtcggatg ggccggtcgg gggggaactc ctcggcgatc acggtgagcg
     1321 tggcgccggc ggccttcccg atccgcacgg ccaggtgccc ggccgaacgg cccatggcca
     1381 gcacgaagaa ccaccgcttc gtggtcatgg cgtccgtcat caggttgttc accagctcga
     1441 ccccgacgtg ccgagccgtc tgaaacccga acgtggagat ccctctggga aggggcaggt
     1501 cgttgtcgat cgtcttcggg acgtgggcga ccttgatgga accgcccgcc gcctcggcga
     1561 ccttcgacgc tgagaacgcg gtgtcgtcgc cgccgatggt gaccagagcg tcgacgccga
     1621 gctccgagag tgccttcacg cagttgtgca tccgccactc cgggtcggcc gcgccctcgt
     1681 cccgaacggt cgggttggcc cgggaggtga acaggagcga accgcccagc acgtggatcc
     1741 gggagacgtc ggtgatcgtg aggcgctgga cctgatcggt tgaccccagg atcaagtgcg
     1801 agaacccgtc gcggatcccg atgacgtccc accccgagtt gatcgcctcg atcgtcgcgg
     1861 cggagatgac ggagttgatc ccgggggccg gtcccccgcc gcacaggatc ccaagccgat
     1921 gccgaccgtc gaccatcgcc atcggccaca gtagcggccg cctgccgctt cgctcaacca
     1981 gacgcctcga tcctccgatc ctcctggaag aagcaccgat cgacatcgga ggcgccggtg
     2041 cgaacgaagt ccctcatcgc cgcgtgcgcg atgctggcga tcctggccgc cggcctggct
     2101 ccgggcgcct ccgccgagca gacgatcgag cggcgcctgt ccgaggccag gacctcccgt
     2161 gaccgggcga accgctcggt gcgggacgcc gaggcccggc tcgccgcgct gaccgccgag
     2221 tacgaacggg tccgctcgga tctcgatctc gccgtccggg acatggtcgt ggcgcaactg
     2281 gcccagcggg gcgccacgga cgatctggca gccgcgcagg acgcgctgga ccggaaggcc
     2341 gccgcggcgt acgaggccgg gcccaccgcc accctcgagc tgatcttcgg ctcggcctcg
     2401 acggcggacc tcgccgcggc cgaagtcttc gcggccagcg cattctccgt cggccagacg
     2461 caggtcgacg aggtggtccg tctgcgcgcc tccttggagg gcgtggccct gcggctggag
     2521 cggaaccgcg cggacgtcgc cgcggccctt cgcgacctcg accgggacac ctccgccgtc
     2581 accgcggagg tcgagcgggc ccgcgcggtc gccgccgacg ccggcctcga ggtcacgaag
     2641 ctggagaagg agcagcgcga gctggaccgg gcccggcgga ggctggaagc ctcccttgcg
     2701 ccgctgatcg acgcgacccg gggccgggac cagagcgagc tgctggcccg gctcgggccg
     2761 agccagggcc gggggtgcga catcccgagc gggctgacgc cgaccggcga caccctcgcc
     2821 ggcgactcgt cgtggtacgg ctgggacttc gcgggcaatc ccaccgccag cggggcgatc
     2881 tacgacccga ggctgttcac cgcggcgaac aaggatctgc cgctcaacgt gttcctgcgg
     2941 atccacttcc gcgacgagtg cgccatcgtc ctggtgaacg accgtgggcc gtacgtgggc
     3001 gaccggatca tcgacgtgtc ccaggcggtc gccgaatacc tcgggtacaa gtcggcgggc
     3061 gtggcctggg tcaccgccga cgtgctcgtc tcgaagtaac cgcgtgagga cgggttcatt
     3121 ggccgatcgc gaagaagccg gtcaggcttc ccgcgtagac ccggtggcca aacccgatgg
     3181 cggggggcgc ccgaacgtag gcgcgatcca ccagaaaggg cccgcctcgg gtctccagta
     3241 ggattccgcg gcgtgccgtc tgtcctggcc gtcgactcgg gtacgaccgg cgtgaccgcg
     3301 ctcgttgtcg acgaggacgg ggcggtccgc ggcaggggct accgcgagtt cccgcagagc
     3361 ttcccccggc cgggatgggt ggagcacgac cccgatcact ggtgggaggc catgctcgcc
     3421 gcgtgctccc aggcgctcgg gggggccggc ctcgacgccg acgacctcgc cgccctgggc
     3481 atcaccaacc agcgggagac gacggtggta tgggagcgcg acgcgctgcg gccgatccac
     3541 ccggccatcg tgtggcagga ccgcaggacg gcgtccctgt gccaacaact cagggaggaa
     3601 ggatgggagg accgcgtccg ggaccgcacc gggctcgtca tcgacccgta cttctcgggg
     3661 acgaagctcg cgtggctgct cgacaacgtc ccgcaggccc gggaggacgc cgaggccgga
     3721 cggctcgcct tcggcaccgt cgatagctac ctgctggcca
//
LOCUS       DASPWO010000140         3682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_192969, whole genome shotgun sequence.
ACCESSION   DASPWO010000140 DASPWO010000000
VERSION     DASPWO010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192969"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VEO00_03270"
     CDS             <1..450
                     /locus_tag="VEO00_03270"
                     /inference="COORDINATES: protein motif:HMM:NF037143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HYU57057.1"
                     /translation="DVSDPYFGKWKESQAEHLRTIRESFQPVPILTARLFDQELVGID
                     LLREMGDEVYGGRNVTDIFHRDEPIVVRKRGPVYVLSLRLPFTDKEDLDIHRKADELF
                     VRVGPYKRNLVLPHTLQRLEVMEASFLDDRLQITFGKSPRRAAASSS"
     gene            489..818
                     /locus_tag="VEO00_03275"
     CDS             489..818
                     /locus_tag="VEO00_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57058.1"
                     /translation="MAESTKRGARSGGTPPGGGPRPAGEEGAAPSRVAHDGGAGTTRK
                     RGPVNCDVALCPVSLILTATGSVQPEAMEHLLAAGRELMLAVKAVIDARAEGWTRTSP
                     LEKIVIE"
     gene            826..1851
                     /locus_tag="VEO00_03280"
     CDS             826..1851
                     /locus_tag="VEO00_03280"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HYU57059.1"
                     /translation="MTRLAIGLDVGGTKVAGLVLAEDGAVLAGEERPAMADDADAALG
                     AVIQLGQDLQKEHEDVVAAGIGAAGLVDHDAGVFRFAPNLSWRELPLRDRVAEALDLP
                     VVLDNDANAAGWGEFRFGAGAGHREVLMVTVGTGIGGAIVTGGRLLRGAHGFAAEIGH
                     IVMQPGGPRCGCGNAGCWEQLASGTAIGRLGREAARDHPASIIAELAGSPERVSGVHV
                     TEAGRRGDHIAAHILAEVGAWLGVGLAGLANVLDPEVVVVGGGVGEIGEPVLGPARRT
                     FREHLEGAEHRPEIPLLEARLGNEAGAIGAAALALDEAGGRLGVARPVERSTRPHPAS
                     EPPQAPR"
     gene            1848..2774
                     /locus_tag="VEO00_03285"
     CDS             1848..2774
                     /locus_tag="VEO00_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU57060.1"
                     /translation="MKVGVILPLFGGDPVRVLDRAQDAEAFGFDGVFAFDHFFPPGAP
                     SDRPALEAFTMLAAVAEGTERVSLGTLVTRASLRPAGLIAKMASMLDWMSGGRMILGI
                     GTGDPIDEPEHVAFGFRTLSVQDRRAHLSEAVRALKALFAGERWGGGEWIPAMDGPLV
                     PEPVQPGGPPVWIGAQADSVVRMAGAIADGWNGWGMDPARYARKVAVLRAAAEEAGRE
                     ELPEPTWAGIVLVGRDRPELEALVEARRARGLPDEAWTGTVDELVHLLDDLAGAGAAW
                     TIMVPAGPRDRWTVIALEVLPRLTRAGSTSGT"
     gene            complement(2680..3150)
                     /locus_tag="VEO00_03290"
     CDS             complement(2680..3150)
                     /locus_tag="VEO00_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HYU57061.1"
                     /translation="MRVLLDTNVLISAFIFPGGAPEAVYRMALRGRIEVVTSATLLAE
                     FGRVLVGKFGWDEARADRAVVQVARVAEVVDPQERVAVVTADPADDRVLEAALEGAAT
                     AIVSGDRHLLRLGSWRGIAIVDPATFLTSNPPSSGAGGPRGRSRSSGRAGRPGP"
     gene            complement(3147..3410)
                     /locus_tag="VEO00_03295"
     CDS             complement(3147..3410)
                     /locus_tag="VEO00_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HYU57062.1"
                     /translation="MAAKNRRSSRVLTVSVPPESADDFERIAEEEGRNRSELFREMLR
                     IYRAYRETGEFESLQRYGAAHARRLGVRDERDVERLIRDARSG"
BASE COUNT          516 a         1273 c         1402 g          491 t
ORIGIN      
        1 gacgtcagcg acccgtactt cggcaagtgg aaggagagcc aggccgagca cctccggacc
       61 atcagggaat ccttccagcc ggtgcccatc ctgaccgcgc ggttgttcga ccaggagctg
      121 gtggggatcg acctgctgcg cgagatgggc gacgaggtct acggcggccg caacgtcacc
      181 gacatcttcc atcgcgacga gccgatcgtc gtgcgcaagc gcggtccggt gtacgtgctc
      241 tcgctgcgcc tcccgttcac cgacaaggag gacctcgaca tccaccgcaa ggccgacgag
      301 ctgttcgtgc gcgtcggccc ctacaagcgc aacctggtcc tgccgcacac gctgcagcgt
      361 ctggaggtca tggaggcgag cttcctggac gaccggctgc agatcacctt cggcaagagc
      421 ccgcggcggg cggcggcctc cagcagctga cccccgcaca cccagcgagg agacgaaagg
      481 acccaccgat ggccgagagc accaagcgag gcgcccgttc cggagggaca ccgcccggcg
      541 ggggcccgcg cccggcgggg gaggaggggg cggcaccctc gcgggtcgcc cacgacgggg
      601 gcgccgggac cacacgcaag cgcggtccgg tgaactgcga cgtcgcactc tgcccggtgt
      661 cgctgatcct gaccgccacc ggcagcgtcc agcccgaggc catggagcac ctgctggcgg
      721 cggggcgcga gctgatgctc gcggtgaagg cggtcatcga cgcccgggcc gaggggtgga
      781 cccgcacgtc gccgctcgag aagatcgtca tcgagtgacg gagccgtgac ccggctggcc
      841 atcggcctcg acgtcggcgg gaccaaggtc gcgggactgg tcctggccga ggacggcgcc
      901 gtcctggccg gcgaggaacg cccggcgatg gcggacgacg ccgacgccgc cctcggcgcg
      961 gtgatccagc tcggtcagga cctgcagaag gagcacgagg acgtcgtggc ggccgggatc
     1021 ggggcggccg ggctcgtgga ccacgacgcc ggggtgttcc gcttcgcccc gaacctgtcg
     1081 tggcgggagc tgccgctgcg cgaccgcgtc gccgaggcgc tcgacctgcc ggtcgtcctc
     1141 gacaacgacg cgaacgcggc cggctgggga gagttccgct tcggggccgg ggccgggcac
     1201 cgcgaggtgc tgatggtgac cgtggggacc gggatcggcg gggcgatcgt caccggcggg
     1261 cgcctcctgc gcggcgcgca cgggttcgcc gcggagatcg ggcacatcgt gatgcagccg
     1321 ggcgggccgc ggtgcggctg cgggaacgcc ggctgctggg agcagctcgc gtccgggacc
     1381 gccatcggcc ggctcgggcg cgaggccgcc cgcgaccatc ccgcgtcgat catcgccgag
     1441 ctggcggggt cgcccgagcg cgtcagcggc gtgcacgtca ccgaggccgg gcgccgcggc
     1501 gaccacatcg cggcgcacat cctggcggag gtcggcgcgt ggctgggggt tggcctggcc
     1561 ggcctggcca acgtgctcga cccggaggtc gtggtggtcg gcggcggcgt cggggagatc
     1621 ggcgagccgg tcctcgggcc cgcgcggcgc acgttccggg agcacctcga gggcgccgag
     1681 caccgcccgg agatcccgct gctcgaggca cggctgggga acgaggcggg cgcgatcggg
     1741 gcggcggccc tggcgttgga cgaggccggg gggcggttgg gggtcgctcg ccctgtggag
     1801 cgatccacca gaccacatcc ggcgagcgaa cccccccagg caccacgttg aaggtcgggg
     1861 tgatcctgcc gctgttcggc ggggacccgg ttcgcgtgct cgaccgggcg caggacgccg
     1921 aggcgttcgg cttcgacggc gtgttcgcgt tcgaccactt cttcccgccc ggcgcgccct
     1981 cggaccggcc ggcgctcgag gcgttcacca tgcttgccgc cgtggccgag ggcaccgagc
     2041 gggtgtcgct cgggacgctc gtcacccggg cgtcgctgcg cccggcggga ctgatcgcga
     2101 agatggcctc gatgctcgac tggatgtccg gcggccggat gatcctcggg atcggcaccg
     2161 gcgacccgat cgacgagccc gagcacgtgg cgttcgggtt ccgcacgctc tcggtgcagg
     2221 accggcgggc ccacctgtcc gaggcggtgc gcgcgctgaa ggcgctcttc gccggggaac
     2281 gctggggcgg cggggagtgg atcccggcga tggacggccc gctggtgccc gagcccgtgc
     2341 agccgggtgg gcccccggtg tggatcgggg cacaggcgga ctcggtggtc cggatggccg
     2401 gggccatcgc cgacgggtgg aacggctggg ggatggaccc ggcgcgctac gcgcggaagg
     2461 tcgcggtgct gcgcgccgcg gcggaggagg ccgggcggga ggagctgccg gagccgacgt
     2521 gggcggggat cgtcctggtc ggccgggacc gccccgagct ggaggcgctg gtcgaggcgc
     2581 gccgcgcccg cggactgccg gacgaggcct ggaccggcac ggtcgacgag ttggtgcatc
     2641 tccttgacga tctcgccggc gccggcgccg cgtggacgat catggtcccg gccggcccgc
     2701 gcgaccgctg gaccgtgatc gccctcgagg tcctcccgcg cctgacgagg gcgggttcga
     2761 cgtcaggaac gtagccggat caacgatcgc gataccccgc cacgacccca gccgtagcag
     2821 gtggcggtcg ccggagacga tcgccgtggc ggccccttcg agtgctgcct cgaggactcg
     2881 gtcatcggcg gggtcggccg tcaccacggc aacgcgctcc tgcgggtcca cgacctccgc
     2941 cacgcgcgcg acctgcacca cggcccggtc cgcccgagct tcatcccagc cgaacttccc
     3001 gacgagaaca cggccgaact ccgcgaggag cgtcgcggac gtgacgacct cgatccttcc
     3061 gcggagcgcc atccgataga cggcctccgg cgcgccgccg gggaagatga aggccgagat
     3121 gaggacgttc gtgtcgagga ggaccctcac ccggaccggg cgtctcggat caggcgttcg
     3181 acgtcccgct cgtcacgaac ccccaggcgg cgagcatggg ccgccccgta gcgctggagc
     3241 gactcgaact cgccggtttc tcggtatgcg cgatagatcc gcagcatctc gcggaagagt
     3301 tcgctgcggt tgcggccttc ctcctccgcg atccgctcga aatcgtcggc agactcgggc
     3361 gggacggaga cggtcaggac tcgagacgat ctccggttct tggcggccat gtcatacgga
     3421 gtattacaga tcggccgaag gctgacaagt cccaacaggg atattcgggc ccgggggatg
     3481 gggctccgca ttcgttgact gaatcggtta ctggacccta ggcttgtccg gctccagccg
     3541 cctctcgagg aaggatttcc gtggcacaga gacgtgtcct gatcatgggc gccgccgggc
     3601 gcgacttcca caacttcaac gtcgtctacc gcgacgaccc cgacaccctg gtggtggggt
     3661 tcaccgcgac tcagatcccc ta
//
LOCUS       DASPWO010000141         5091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_444314, whole genome shotgun sequence.
ACCESSION   DASPWO010000141 DASPWO010000000
VERSION     DASPWO010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444314"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1390)
                     /locus_tag="VEO00_03300"
     CDS             complement(107..1390)
                     /locus_tag="VEO00_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HYU57063.1"
                     /translation="MKAEVLGVGTEILLGQIPNSNAQWISERLAGIGVDVLHHQAVGD
                     NEARIAEAFLLALSRADVVIATGGLGPTQDDVTREGLSAALGLRLVPDPSIADWLGDR
                     FAALGRRMPESNLRQAMVPEGARTIMPQRGTAPGLMIEADGKRVYALPGVPAEMKEMM
                     EGAVLPELATLAGPAALVSRVLKVTGMAEASVAEALDDLFRGSENPTIAYLASSGEVK
                     VRVTAKAPTRAEAEALIEPVAAEVSRRLGDRVFTRDDEALELVVGRLLKARQVTVATA
                     ESMTGGSLAARLTAHPGSSAFFVGSAVVYTEAAKRDVLGVSRATLDGPGVVSEECARE
                     MARGARRLFGANLGVSLTGVAGPEEHGDKPPGTVWVGLAAEGAEEARTFRAPGDRAQV
                     RRWAEQAALDLLRRHLEGLGEGPLGPDTAFDATQA"
     gene            complement(1401..1982)
                     /gene="pgsA"
                     /locus_tag="VEO00_03305"
     CDS             complement(1401..1982)
                     /gene="pgsA"
                     /locus_tag="VEO00_03305"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HYU57064.1"
                     /translation="MTPLGLGWPNLVSILRIVLAPVLVALVAADTRPASYAAGAVFAV
                     GAITDGLDGYLARRYDSVTRTGQWLDPLSDKILVAAPVIAMTALDRFPWWATVVIVTR
                     ELAVSVLRAWLGTRGRAMPASWLGKLKTAAQLLAILLYLIPGAVDALDPAPFVALVMA
                     VGLTVWSGVDYAVKAMRPRPAVPPSSEVRPVAS"
     gene            complement(1979..2758)
                     /locus_tag="VEO00_03310"
     CDS             complement(1979..2758)
                     /locus_tag="VEO00_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HYU57065.1"
                     /translation="MLVRAHGRPEVCLNASGTGIGRALRKAREVRGKSLEEASRETRI
                     RAEYLHALEREAFEGLIGDVYVRGFLRSYSSYLGLDADKVLGVFNRSHGGPADGPASP
                     APRPTGALTHPNGHPLIHRTGRWGIAVAVAVVVLIAVAAIALLGGSGEPEATGALATT
                     PTGPALESGASVVANLRALQEVQATITVDGRVEFEGLLERGEARSLVGQESIELALAE
                     GGVVDLQVNEVEIGTPGEPGEPYTVVFRPQDYRNTPSPPGG"
     gene            2773..3204
                     /locus_tag="VEO00_03315"
     CDS             2773..3204
                     /locus_tag="VEO00_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYU57066.1"
                     /translation="MADIDPGMLKRVTVFSSLTPDQLEKLAAAATLTVHAEGEEIASE
                     GTVGRRFHLLLDGGAVVERGRERVATVRAGEFVGEVALLGGGKSTATVRCTAPTRCLT
                     IEREPFWALLEEEPAIALRILEIVCRRLELQALPATANLSG"
     gene            complement(3214..>5091)
                     /locus_tag="VEO00_03320"
     CDS             complement(3214..>5091)
                     /locus_tag="VEO00_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HYU57067.1"
                     /translation="LPAGGKPQPGRGVRRVREAGGLVGALTAWPLSRLVSVYGAAIVT
                     LGLALLGILVFTATPLTNVGAGLRRFFVREADEAGQGEDQEEGRRRHRRVRLPEPEPE
                     DEEQPGIAAVMAGPVPEPPPVTAPEPRQMALPVSGRGGYKLPPLDLLRRAPDSTATGR
                     DEEQVMSRLERTFTDFGVPARVTAAHRGPTVTLYEVEVDAGTKVNKVLSLSDDIAYAL
                     ATQDVRLIAPIPGKSAIGVEVPNKVRDLVMLGDVLSARVAKETKHPLAVSLGKDVHGR
                     PVMVNLTEMPHLLVAGSTGAGKSSLINAFVTSVLMRATPEDVRMVLVDPKRVELSHFA
                     DLPHLLSPVIVHPKRAAEALNWVVREMELRYESLATVAQRDIAAYNQALGEGTLRIPP
                     GQEDAFHRLPYILVVIDELSDLMMIAPRDVEDAICRIAQMARAVGIHLVVATQRPSVD
                     VVTGLIKANIPSRLAFMTASQADSRVILDVGGAEKLVGRGDMLFLPANASRPHRIQGA
                     WVTEPEIESITEWCRTQRKPSYLQWVEGLGKPPTDEELSSEGDDDEALIEAAADLVIR
                     SQLGSTSMLQRKLKVGFARAGRIMDALEERGIVGPSQGSKARDVLLSYEEWMESRSAR
                     T"
BASE COUNT          689 a         1933 c         1788 g          681 t
ORIGIN      
        1 gccgtggtgc cggaggtggt gcagaggatc gcgacgtcct cgccttgggt cgcatccacc
       61 agttgatcgt aaagtcttgg ctcccgcgcg gcgcggtcgc gccccatcag gcctgcgtcg
      121 cgtcgaacgc ggtgtcgggg cccagcgggc cctccccaag gccctcgagg tgccggcgca
      181 gcaggtcgag ggccgcctgc tcggcccacc gccgcacctg ggcgcggtcg cccggcgccc
      241 ggaacgtccg cgcctcctcg gccccctcgg cggcgaggcc gacccacacc gtcccgggcg
      301 gcttgtcccc gtgctcctcg gggccggcga ccccggtcag cgagaccccg aggttcgcgc
      361 cgaacagccg ccgcgcgccg cgcgccatct cccgggcgca ttcctcgctg accacgcccg
      421 gtccgtcgag cgtcgcccgg gagacgccga gcacgtcgcg cttcgccgcc tccgtgtaca
      481 ccaccgccga tccgacgaag aacgcgctcg acccggggtg cgccgtcagg cgcgcggcga
      541 gcgagccgcc ggtcatgctc tcggccgtgg ccaccgtcac ctgccgcgcc ttcagcaggc
      601 gcccgaccac cagctcgagg gcctcgtcgt cgcgcgtgaa cacccggtca ccgagccggc
      661 gcgacacctc ggcggcgacc ggctcgatca gcgcctcggc ctcggcccgc gtcggcgcct
      721 tcgccgtcac ccgcaccttc acctcgcccg aggacgccag gtacgcgatg gtcgggttct
      781 ccgagccccg gaacaggtcg tcgagcgcct cggccaccga ggcctcggcc atcccggtca
      841 ccttcagcac gcggctgacc agggcggcgg gcccggccag cgtggccagc tcggggagca
      901 ccgcgccctc catcatctcc ttcatctctg ccggcacgcc cgggagcgcg tagacgcgct
      961 tcccgtccgc ctcgatcatc agcccgggcg ccgtcccgcg ctgcggcatg atcgtccgcg
     1021 ccccctcggg gaccatcgcc tggcgcaggt tcgactctgg catccgcctg ccgagggccg
     1081 cgaaccgatc gcccagccag tcggcgatcg acgggtccgg aaccaagcgc agcccgagcg
     1141 ccgcggaaag gccctctcgg gtgacgtcgt cctgggtcgg accgaggccg ccggtcgcga
     1201 tcaccacgtc ggcgcgggac agcgccagga ggaaggcctc cgcgatccgc gcctcgttgt
     1261 ccccgacggc ctggtggtgc agcacgtcca cgccgatgcc ggccagccgc tcggagatcc
     1321 actgcgcgtt cgagttgggg atctggccga gcaggatctc ggtgccgacc cccaggacct
     1381 cggccttcat tcgctccacc tcacgacgcg acgggccgca cctcggatga cggcgggacc
     1441 gccgggcggg gtcgcatcgc cttcaccgcg tagtcgacgc ccgaccacac cgtcagtccg
     1501 accgccatca cgagcgccac gaacggcgcc gggtcgaggg cgtcgaccgc gccggggatc
     1561 aggtacagca ggatcgccag cagctgggcg gccgtcttca gcttcccgag ccacgacgcc
     1621 ggcatcgccc gcccgcgggt cccgagccag gcgcgcagca ccgacaccgc cagctcccgg
     1681 gtgacgatca ccaccgtcgc ccaccacggg aagcggtcca gtgcggtcat cgcgatgacc
     1741 ggagccgcga ccaggatctt gtccgagagc gggtcgagcc actggccggt gcgggtcacc
     1801 gagtcgtagc ggcgagcgag atagccgtcc aggccgtcgg tgatcgcgcc gacggcgaac
     1861 accgccccgg ccgcgtacga ggccgggcgc gtgtcggcgg cgacgagcgc gacgagcacg
     1921 ggagccagca cgatgcgaag gatcgagacg aggttcggcc aacccaggcc gagcggggtc
     1981 accctccggg cggcgatgga gtgttccggt agtcctgcgg gcggaacacc acggtgtagg
     2041 gctcgccggg ctctccgggc gttccgatct ccacctcgtt cacctgcagg tccaccacgc
     2101 caccctccgc cagggccagc tcgatcgact cctgcccgac gagggaccgg gcctcacctc
     2161 gctcgagcag gccctcgaac tcgacccgcc cgtccacggt gatcgtcgcc tgaacctcct
     2221 gcagcgcgcg caggttggcg acgacggacg cgccggactc gagcgcggga ccggtgggtg
     2281 tggtggccag ggcgccggtc gcttccggct cgccggagcc tccgagcagc gcgatggcgg
     2341 cgacggcgat gagcaccacg acggcgaccg ccacggcgat gccccaccgc ccggtgcggt
     2401 ggatcagcgg gtgcccgttc ggatgggtca gcgcgccggt cgggcgcggg gccggcgagg
     2461 ccgggccgtc cgccggcccg ccgtgcgacc ggttgaacac gccgaggacc ttgtcggcgt
     2521 ccaggccgag gtagcttgag taggagcgaa ggaagccgcg gacgtacacg tcgccgatca
     2581 ggccctcgaa cgcctcacgc tccagggcgt gcaggtactc ggcgcggatg cgcgtctccc
     2641 ggctggcctc ctcgaggctc tttccgcgga cctcgcgggc cttgcggagg gcccggccga
     2701 tgccggttcc ggacgcgttc aggcagacct ccggacgtcc atgagcacga acgagtatgg
     2761 caggatggcc tgatggccga catcgacccg ggcatgctga agcgggtcac ggtcttctcg
     2821 tcgctcaccc ccgaccagct cgagaagctg gcggccgcgg ccacgctcac ggtgcacgcg
     2881 gagggcgagg agatcgcgag cgagggcacg gtcggccgcc ggttccacct gctcctcgac
     2941 ggcggcgccg tggtggagcg cggccgggag cgggtcgcga cggtacgcgc cggggagttc
     3001 gtcggcgagg tcgccctgct gggcggcggg aagtcgaccg ctaccgtccg ctgcaccgcc
     3061 ccgacgcgct gcctgacgat cgagcgcgag ccgttctggg cgctgctgga ggaggagccg
     3121 gcgatcgcgc tgcgcatcct ggagatcgtc tgccgcaggc tggagctcca ggccctcccc
     3181 gccaccgcca acctctccgg ctgacgcctt tcgtcacgtc ctggccgaac gggactccat
     3241 ccactcctcg taggacagca ggacgtcgcg ggccttcgag ccctggctcg ggccgacgat
     3301 gccccgttcc tccagcgcgt ccatgatccg gccggcccgg gcgaacccga ccttcagctt
     3361 gcgctgcagc atcgaggtcg acccgagctg cgagcggatc accaggtcgg cggccgcctc
     3421 gatcagcgct tcgtcgtcgt cgccctcgct cgacagctcc tcgtcggtcg gcggcttccc
     3481 gaggccctcg acccactgca ggtaggacgg cttgcgctgc gtacggcacc actcggtgat
     3541 cgactcgatc tccggctcgg tgacccaggc gccctggatg cgatgcggcc tcgacgcgtt
     3601 ggccggcagg aagagcatgt cgccgcgccc gacgagcttc tcggccccgc cgacgtcgag
     3661 gatcacccgc gagtctgcct gcgacgccgt catgaacgcg agccgcgagg ggatgttcgc
     3721 cttgatcagg ccggtgacga cgtccaccga cgggcgctgt gtcgccacca ccaggtggat
     3781 gccgaccgcg cgggccatct gcgcgatccg gcagatcgcg tcctcgacgt cgcgcggggc
     3841 gatcatcatc aggtcggaga gctcgtcgat gacgacgagg atgtacggca ggcgatggaa
     3901 ggcatcctcc tggccggggg ggatgcgcag cgtcccctcc cccagcgcct ggttgtaggc
     3961 ggcgatgtcg cgctgcgcga ccgtggccag gctctcgtac cgcagctcca tctcgcgcac
     4021 cacccagttc agggcctcgg cggcgcgctt cgggtgcacg atgaccggcg acagcaagtg
     4081 cggcaggtcg gcgaagtgcg agagctccac gcgcttcggg tcgacgagca ccatgcgcac
     4141 gtcctcgggc gtggcgcgca tcagcacgct ggtgacgaac gcgttgatca ggctggattt
     4201 ccccgccccg gtcgagccgg cgacgagcag gtgcggcatc tcggtcaggt tcaccatcac
     4261 cggccgcccg tggacgtcct tgccgagcga caccgccaga gggtgcttcg tctccttcgc
     4321 cacccgggcc gacagcacgt ccccgagcat caccaggtcg cgcaccttgt tcggcacctc
     4381 gacgccgatc gccgacttcc ccgggatcgg cgcgatcagc cggacgtcct gggtggccag
     4441 ggcataggcg atgtcgtccg acagcgacag caccttgttc accttcgtcc cggcgtcgac
     4501 ctcgacctcg tagagcgtca cggtcgggcc gcggtgtgct gcggtcaccc gggccggcac
     4561 gccgaagtcg gtgaacgtcc gctcgaggcg gctcatcacc tgctcctcgt cgcggccggt
     4621 ggcggtcgag tcaggagccc ggcggagcag gtcgagcggc ggcagcttgt agccgccgcg
     4681 gccggacacc ggcagggcca tctgccgcgg ctcgggcgcg gtgaccgggg gcggctccgg
     4741 gaccgggccg gccatcaccg ccgcgatgcc gggctgctcc tcgtcctcgg gctccggctc
     4801 gggcaggcgc acgcgcctgt ggcggcgacg cccctcctcc tggtcctccc cctggccggc
     4861 ctcgtcggcc tcccggacga agaagcgacg cagcccggcg ccgacgttcg tcagcggtgt
     4921 ggccgtgaac accaggatcc cgagcagcgc cagcccgagg gtcacgatcg ccgcgccgta
     4981 cacggacacg aggcgcgaca gcggccaggc ggtcagcgcg ccgaccaggc ccccggcctc
     5041 ccgaacccgg cgtaccccgc gcccgggctg gggtttcccg ccagcaggga g
//
LOCUS       DASPWO010000142         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_444327, whole genome shotgun sequence.
ACCESSION   DASPWO010000142 DASPWO010000000
VERSION     DASPWO010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444327"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="VEO00_03325"
     CDS             complement(<1..1327)
                     /locus_tag="VEO00_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57068.1"
                     /translation="MQKIRLDELPARPAGRLRRILSPSARHQTGPETAPSGPIIHLTE
                     ARPPRGDPPTLEQALMARIDGGLRRVERTAARLMNEIAAEVWRAVGPEAGERQEERVL
                     AVLARDQALKGSIAHADERFQAIDLRIGRIEQALQALSGAVRAMPEQLRGDLGSVRAD
                     LAEDVARVATGTEARLAGSAATFARSAEHLRAGMRELATGRATRDGEALQRVERVERA
                     AEDLGHRADKGLARIERTAAEVGMQLKEAISGLDARLAELDRSVGERIERGEAAVAER
                     LSGALEIVARRAEASIEATGSRADAAFNRALRRLDAGMAAAARRTNELLVDGVQRLEA
                     SRNETADAVGAAVAAAQARTGEAVRAALERTARIELQVSSLAARAKELPSSDRLDTAL
                     VSMSDRIADLDRAIAGLAGRDEREDRNVAERLESATATFVERFESLAEAL"
     gene            1482..1558
                     /locus_tag="VEO00_03330"
     tRNA            1482..1558
                     /locus_tag="VEO00_03330"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1516..1518,aa:Trp,seq:cca)
     gene            1569..1844
                     /gene="secE"
                     /locus_tag="VEO00_03335"
     CDS             1569..1844
                     /gene="secE"
                     /locus_tag="VEO00_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HYU57069.1"
                     /translation="MNRQTKRMMGKRQAAERLQRPRPMPAEKKKRVGPRQFLREVRQE
                     LKKVAWPSRKELFAYTVVVLVAVVVMTSLVFGMDFLFSKAVLQVFGT"
BASE COUNT          248 a          743 c          744 g          268 t
ORIGIN      
        1 ctagggcctc ggccagcgac tcgaaccgtt cgacgaaggt cgcggtggcg gactccaggc
       61 gttcggccac gttccggtcc tcgcgttcgt cgcggccggc caggcccgcg atggcccggt
      121 ccaggtcggc aatccgatcg ctcatcgaca cgagcgccgt gtccaggcgg tcggacgacg
      181 gcagctcttt ggcccgggcg gccagcgagc tgacctgcag ctcgatccgc gcggtgcgct
      241 ccagggcggc gcggacggcc tcaccggtac gggcttgtgc cgccgcgacg gccgccccga
      301 cggcgtcggc cgtctcgttc cgggaggcct ccagccgctg cacgccgtcc accagcagct
      361 cgttcgtccg gcgggcggcc gccgccatcc ccgcgtcgag gcggcggagc gcgcggttga
      421 acgccgcgtc ggcgcgcgag ccggtggcct cgatcgaggc ctccgcgcgg cgggccacga
      481 tctccagggc cccgctcagc cgctccgcga cggcggcctc gccccgctcg atccgctcgc
      541 cgaccgatcg gtccaattcg gcgagacggg catcgaggcc ggagatcgcc tccttgagct
      601 gcatgcccac ctcggcggcc gtgcgctcga tgcgcgccag gcccttgtcg gcgcgatggc
      661 cgaggtcctc ggcggcccgc tcgacccgct cgacccgctg cagggcctcg ccgtcgcggg
      721 tggcccgccc ggtggccagc tcgcgcatcc cggcccgcag gtgctcggcc gagcgggcga
      781 acgtggccgc cgaccccgcc aggcgggcct cggtgccggt cgcgaccctg gcgacgtcct
      841 cggccaggtc ggcccggacg ctcccgaggt cgccccgaag ctgctccggc atcgcccgga
      901 cggcgccgga gagggcctgc agcgcctgct cgatgcgacc gatgcgcagg tcgatggcct
      961 ggaagcgctc gtcggcgtgg gcgatcgagc ccttcagggc ctggtcgcgg gcgagcacgg
     1021 ccaggacgcg ctcctcctgc cgctcgcccg cctcgggccc gaccgcccgc cacacctcgg
     1081 cggcgatctc gttcatcagc ctggcggccg tgcgctcgac gcggcggagc ccgccgtcga
     1141 tccgcgccat cagggcctgc tccagggtcg gaggatcgcc ccgcgggggg cgggcctcgg
     1201 tgaggtggat gatcggcccc gacggtgccg tctcgggacc ggtctggtgg cgcgccgaag
     1261 gagacaggat ccggcggagc ctgccggccg gacgcgccgg gagctcgtcc aggcggatct
     1321 tctgcatggt cgcttcgctc tccggggccg gcgcgcaagc tcgcgagcag tgtaggagag
     1381 cgaacctgcc gaatcacgca cctcgagggg atcctttgct gcctgctgga ggcggggcct
     1441 atactcgtct cggtcccggg ccccggagcc cctccgcacc caggggcgta gctcgaattg
     1501 gtagagcgcc ggtctccaaa accggtggtt gggggttcga gtccctccgc ccctgccaga
     1561 ggagagagat gaacaggcag accaagcgga tgatggggaa gcggcaggcc gccgagcggc
     1621 tgcagcggcc gcgtcccatg ccggcggaga agaagaagcg cgtcgggccc aggcagtttc
     1681 tgcgcgaggt gcgccaggag ctgaagaagg ttgcctggcc gagccgcaag gagctgttcg
     1741 cctacaccgt cgtcgtactg gtggccgtgg tggtgatgac ctcgctcgtc ttcgggatgg
     1801 acttcctgtt ctcgaaggcg gtcctgcagg tcttcggcac ctagccccgc acgcggatcc
     1861 cgagagccat ggaagacccc gatcggacga gcatgaccga cgaccggacg ccccggccgg
     1921 agtccgacgc gcccgccggc gggccggaga cgcccggcgc cgcgcccggc gtccccgcgg
     1981 atcgggacgg ggtcgacgcg ccg
//
LOCUS       DASPWO010000143         5222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_22825, whole genome shotgun sequence.
ACCESSION   DASPWO010000143 DASPWO010000000
VERSION     DASPWO010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22825"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VEO00_03340"
     CDS             complement(<1..187)
                     /locus_tag="VEO00_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57070.1"
                     /translation="MPSRPGQCRRIALALGCVLLTAGAVVAEEAAPTRVLDWETGAGK
                     SYWIPALEVGGFIFGLNQ"
     gene            complement(208..960)
                     /locus_tag="VEO00_03345"
     CDS             complement(208..960)
                     /locus_tag="VEO00_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYU57071.1"
                     /translation="MRFDGRVVVVTGAAKGIGAATAEAFGRERARVAALDIDGAGVDS
                     VVAGLSGRGVDALGVKTDVTSSTAVQAALDAVLSRWGRVDVLVNNAGGFAVMRTFEDT
                     QESEWEAIVRSNLTSVFLVCKAVLPVMKRQKAGRIVNLASVVARGGAVRVPAHYAAAK
                     AGVIALTRMLALEVADLGITVNAVAPGTTATDRVIAARTPEETARVAAAIPLGRLGRP
                     AEIAEAILFLASDAASFVTGAVLDVNGGQEMA"
     gene            complement(991..1362)
                     /locus_tag="VEO00_03350"
                     /pseudo
     CDS             complement(991..1362)
                     /locus_tag="VEO00_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(1375..1509)
                     /locus_tag="VEO00_03355"
     CDS             complement(1375..1509)
                     /locus_tag="VEO00_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57072.1"
                     /translation="MHPELVVGGKIPDLELTDHRSQRVSLSALAQGFPLILTFYRGYW
                     "
     gene            complement(1527..3320)
                     /locus_tag="VEO00_03360"
     CDS             complement(1527..3320)
                     /locus_tag="VEO00_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HYU57073.1"
                     /translation="MARPLLLSCEAVSKAFGTRSLFDDLSFGLFEGDQAGLVGPNGSG
                     KSTLLKILAGLETPDRGSRSLRGGIRIGYVPQDPVFTAGQTVAAVVTAALAGDDEDDR
                     SGRLAQALGRSGFTDGRAGVDTLSGGWKKRLAIARELAAAPDILLMDEPTNHLDVDGI
                     LWLEDVLTERARAFLVVSHDRYFLEHVSTRMLELNRVYPTGLFETEGRYSEFLARRDE
                     FLRGQAAYQESLANTVRREIEWLRRGAKARSTKAKGRIKEAGRLVEELDDRRARSVTA
                     TAGIDFTSSQRRTRRLVVARGLNKSLGGRRLVSDLDLVITPGTRVGLIGPNGSGKTTL
                     LNVLAGTLPPDAGEVEHAEALRVVRFEQERGGLDPALSLRRALAPEGDAVTWQGRSVH
                     VASWAKRFLFRPEQLEAPVGRLSGGEQARILIARLMREPADLLILDEPTNDLDIPTLE
                     VLEDSLAEFDKGLVLVTHDRFMLERVSTVILALDGEGGTATFADYSQWEAARGAAAPA
                     PRKMAERAPERERPRPKRLGYREQREWDGMEQAILDAEGALETCQREMEDPAIASDPT
                     ALQQRAAALQAARAEVDRLYARWAELETKRA"
     gene            complement(3443..4291)
                     /locus_tag="VEO00_03365"
     CDS             complement(3443..4291)
                     /locus_tag="VEO00_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57074.1"
                     /translation="MTALGLSRIAGAVAIVAVLVVTAMVTWSVVRQSDHESPPQVAGG
                     PALGDMTGYVGRVDREGRTIDVSTSLPGVRPVILNVTNDTSIMVRGKQGGLADLSKDM
                     PVRVSYEVRDDVKYVTSIQVVTEEVQASRESETKPPVETKPPVEAKPPAEASPPAEVK
                     APVEAKAPVEVKPPVEAKPAPTVVSPAPTATRPPATAPPVTAATPKPTPPALEPVKPR
                     AREAPPASTQAAGPPTAKPPRIPAATTEAATVPAPRVSTPRPAAADAGDGSAAIDWLL
                     EESRKR"
     gene            complement(4314..>5222)
                     /locus_tag="VEO00_03370"
     CDS             complement(4314..>5222)
                     /locus_tag="VEO00_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HYU57075.1"
                     /translation="EVVFDVLAFPINPADVSMCRGSYRLRPTLPATPGAECIGRVSAV
                     GAGVTHVRAGDLVINLQRENWAQRRRVKGDDVLAIPAGIDVLQGAMLRINPPTALLLL
                     SDVVALKPGDWIIQNVANSAVGRLVIRLARVRGVKTLNVVRRASLFAELRALGADACV
                     VDGPDLAETVKAQTGGAPIRLGLDAVSGRATARLSSCVGEGGVVCNYGSMSGEDPVMA
                     RSALTGNGQTLVGFILGRGLATRSLAQIRAIYADLGEQIVKGVLSAPVEKVYPIEEIK
                     AALAHAQQGERSGKILVVANDWEGAR"
BASE COUNT          745 a         1877 c         1814 g          786 t
ORIGIN      
        1 attgattgag cccgaagatg aacccgccga cctcgagggc cggaatccag tagctcttgc
       61 cggcgccggt ctcccaatcc agcacccggg tgggcgcggc ttcctccgcg accaccgcgc
      121 ccgccgtcag gaggacacac ccgagggcga gggcgattcg tcggcactgg ccgggccgcg
      181 acggcatggc cgtcaggcta acacactcta cgccatctcc tggccaccat tgacgtcgag
      241 gacggcgccg gtgacgaagc tcgcggcgtc ggaggcgagg aagaggatgg cctcggcgat
      301 ctcggcgggc cgaccgagcc gtcccagcgg gatggccgcc gccacccgcg ccgtctcctc
      361 cggcgtgcgg gcggcgatca ctcgatccgt cgcggtcgtt cccggggcca ccgcgttgac
      421 cgtgatgccg aggtcggcca cctcgagcgc cagcatccgg gtcagcgcga tcactcccgc
      481 cttcgccgcg gcgtagtggg ccggaacgcg cacggcaccg ccccgggcca cgaccgaggc
      541 gaggttcacg atgcgccccg ccttctggcg cttcatgacg gggagcacgg ccttacagac
      601 caggaagacg ctcgtgaggt tcgatcggac aatcgcctcc cactccgact cctgggtgtc
      661 ctcgaatgtc cgcatgacgg cgaagccacc ggcgttgttc acgaggacgt cgacgcgacc
      721 ccaccgggac agcacggcgt cgagggccgc ttgaacggcc gtcgacgagg tgacgtcagt
      781 cttgacgccg agcgcgtcga cgccacggcc cgacagcccg gccacgaccg agtcgacgcc
      841 ggcgccgtcg atgtcgaggg ccgccacccg tgcccgctcg cgaccgaacg cctccgcggt
      901 cgccgcgccg atccccttgg cggcgcccgt cacgaccacc acgcgtccgt caaacctcat
      961 cgcttgcctg cgtgcccgat ccgggccgtc tcaacgcgag ggtccatcgt agctgtagtc
     1021 gggccggcac gcctgcatga gcgcgcggag gtcctggcgg agctcctcga gggtcggccg
     1081 tccgacgaac caccagccgt tgtagacctt gtggatcgtg aggtccggcg ccagcgaaaa
     1141 cgagtacggc accgcgacgt caccgtgcgc cgtgttggtg acctcgacga tgtcgagcgc
     1201 ggtgatcacc ttacggtcgt ggtcgctgag gaacgcgaag ctggccccga gccccgcccg
     1261 gaaggccgcg cagacgtccg gcgggtcgac gctcacggcg gcgagatgac agtagttgac
     1321 cttcagctcg tcctggaagg agacgtagtg ccgcaactgg cactcacact tcggtcacca
     1381 gtagccgcgg tagaacgtga ggatgagcgg gaagccctgg gcgagcgccg acaggctcac
     1441 gcgctggctc cggtgatcgg tgagctcgag gtccggtatc tttccgccaa cgaccagctc
     1501 gggatgcatg gcgctcctcc tgacggctac gcccgtttgg tctccagctc ggcccagcgc
     1561 gcatagagcc ggtccacctc cgcccgcgcg gcctgaagcg ccgcggcccg ctgctggagc
     1621 gcggtcgggt ccgaggcgat cgccgggtct tccatctcac gctggcaggt ctccagggcg
     1681 ccctcggcgt ccaggatcgc ctgctccatg ccgtcccact cccgctgctc acgatacccg
     1741 agacgcttgg gccgtggtcg ctcgcgctcg ggggcccgct cggccatctt gcgcggcgcc
     1801 ggagcagcgg cgccgcgggc ggcttcccat tgagaatagt cggcgaacgt cgcggtcccg
     1861 ccctccccgt cgagggcaag gatcacggtg gacacgcgct cgagcatgaa gcggtcgtgg
     1921 gtgacgagga cgagcccttt gtcgaactcg gccaggctgt cctcgagcac ctccagggtc
     1981 gggatgtcga ggtcgttggt gggctcgtcg aggatgagca ggtccgcggg ctcgcgcatc
     2041 aggcgcgcga tcaggatgcg cgcctgctcg ccgcccgaca ggcggcctac cggcgcctcg
     2101 agctgctcgg gtcggaacag gaagcgcttg gcccacgagg cgacgtggac gctgcggccc
     2161 tgccaggtga cggcgtcgcc ctcgggcgcg agggcgcggc gaaggctcaa cgccggatcg
     2221 aggccaccgc gctcctgctc gaatcgcacc acgcgcaagg cttccgcgtg ctcgacctca
     2281 ccggcgtcgg gcggcagcgt gcccgccagc acgttgagca gcgtcgtctt gccgctgccg
     2341 ttggggccga tcagcccgac gcgcgtgcca ggcgtgatga cgaggtcgag atcgctgacg
     2401 agccgccgtc cgcccagcga cttgttcagg ccgcgcgcga ccaccagccg gcgcgtccgg
     2461 cgctgcgagg acgtgaagtc gatcccggcc gtggccgtga cgctgcgcgc ccggcggtca
     2521 tccagctcct ccacgagccg gccggcctcc ttgatgcgcc ccttggcctt ggtcgagcgc
     2581 gccttggcgc cgcgccggag ccactcgatc tcccgtcgca cggtgttggc cagggattcc
     2641 tggtaggcgg cctgcccgcg caggaactcg tcgcggcggg ccaggaactc gctgtagcgc
     2701 ccctcggtct cgaacaggcc ggtgggatag acgcggttca gctcgagcat gcgcgtgctc
     2761 acgtgctcca gaaaatagcg gtcgtggctc accacgagga aggcccgcgc gcgctcggtg
     2821 agcacatcct cgagccagag gatgccgtcc acgtcgaggt gattggtggg ctcgtccatc
     2881 aggaggatgt cgggcgcggc cgccagctcg cgcgcgatgg ccaagcgctt cttccagcct
     2941 cccgaaagcg tgtcaacgcc cgcgcgcccg tccgtgaatc cgcttcggcc gagggcctgc
     3001 gcgaggcggc cggaccggtc gtcctcgtcg tcgccggcca gcgccgccgt cacgacggcg
     3061 gcgacagtct gcccggccgt gaagacggga tcctggggca cgtagccgat ccggatgccg
     3121 ccgcgcaggg agcgggagcc gcgatctggc gtctcgaggc cggcgagaat cttgaggagc
     3181 gtggacttgc cggagccgtt ggggccgacg aggccggcct ggtcgccttc gaacaatccg
     3241 aacgacagat cgtcgaagag cgaccgggtc ccgaaggcct tgctcacggc ctcacagctc
     3301 agcaggagcg gacgggccat ccggcgttac ttcttcttct gcttcggctt cttcttttcc
     3361 ttccgcatgc cggtgccttt ggccatgagc tcaccccctc tcgtcgggcc acctcgtgat
     3421 tatagacggc gaccgccgcg attcagcgct tacgcgattc ctcgaggagc cagtcgatcg
     3481 ccgcgctgcc gtcgccggcg tccgccgcgg cggggcgagg cgtcgagact cgcggggcag
     3541 ggacggtggc cgcttcagtc gtcgcggcgg ggatgcgcgg gggcttcgcc gtgggaggtc
     3601 cggccgcttg ggtcgacgcc ggcggcgcct cccgcgcacg cggcttgacc ggctccagcg
     3661 ccggcggggt cggcttggga gtcgccgcag tgacgggcgg cgccgttgcc ggcggccgcg
     3721 tcgccgtcgg agctggcgac accactgtcg gagccggctt ggcctcgacc ggtggcttga
     3781 cctcaaccgg agccttggcc tcaaccggag ccttgacctc ggccggggga ctggcctcgg
     3841 ccggaggctt cgcctcgact ggcggcttcg tctcgaccgg aggcttggtc tcgctctccc
     3901 gcgaggcctg cacttcttcg gtgacgacct ggatcgaggt cacgtatttg acgtcgtcgc
     3961 gaacctcgta ggacaccctc accggcatgt ccttcgacag gtccgcgagt ccgccctgct
     4021 tgccacggac catgatggac gtgtcgttgg tcacgttcag gataacgggg cgaacgccgg
     4081 gcaagctcgt cgagacgtcg atcgtccgcc cctcgcgatc gacccgtccg acgtacccgg
     4141 tcatgtcgcc gagcgccggt cctccggcga cctggggcgg ggattcgtga tccgactgcc
     4201 ggactacgga ccacgtcacc atcgcggtga ccaccagcac ggcgacgatc gcgacggcgc
     4261 cggcaatccg agagagcccc agcgccgtca ttggttcagc gctccctggg ttgtcatcgc
     4321 gctccctccc agtcgttcgc aaccaccagg atcttaccgc tgcgctcgcc ttgctgggcg
     4381 tgggccagcg cggccttgat ctcttcgatc gggtagacct tctcgaccgg cgccgacagg
     4441 acgcccttga cgatctgctc gccgaggtcg gcgtagatcg cccggatctg cgccagcgag
     4501 cgcgtggcca ggccgcggcc gaggatgaac ccgaccagcg tctgcccgtt accggtcagc
     4561 gcactgcgcg ccatcaccgg gtcctcgccg ctcatcgagc cgtagttaca gacgacgccg
     4621 ccctcgccga cgcaggacga cagccgggcg gtggcccggc cggagacggc gtcgaggccg
     4681 aggcggatcg gggcgccccc ggtctgcgcc ttcaccgtct cggcgagatc cgggccgtcg
     4741 accacgcagg cgtcggcgcc cagcgcccgc agctcggcga acagggacgc gcgccgcacc
     4801 acgttcagcg tcttgacgcc gcgcacccgg gcgagccgga tgaccagacg gccgaccgcc
     4861 gagttggcga cgttctggat gatccagtca cccggcttca gcgcgacgac gtcggagagc
     4921 agcagcaggg ccgtcggcgg gttgatgcga agcatcgcgc cctgaagcac gtcgatcccg
     4981 gccgggatcg cgagcacgtc gtcacccttc acgcgccggc gctgggccca gttctcgcgc
     5041 tgcaggttga tgaccaggtc gccagctctc acgtgcgtca cgccggcgcc gaccgcgctg
     5101 acacgcccga tgcactcggc gcccggggtg gccggcagcg tcggtcggag ccgataactg
     5161 ccccggcaca tcgacacgtc ggcgggattg atcgggaacg cgagcacgtc gaacaccacc
     5221 tc
//
LOCUS       DASPWO010000144         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_278814, whole genome shotgun sequence.
ACCESSION   DASPWO010000144 DASPWO010000000
VERSION     DASPWO010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278814"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VEO00_03375"
     CDS             <1..551
                     /locus_tag="VEO00_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57076.1"
                     /translation="PTRSAEGPASGRKGGPASGSAGQTGGTTSPGTTPGNEMAFLDNE
                     EPIDNDGRAAGEALAQRYKSDQGGSRGSTYGTTGRFRQRERNPRPNSPNELAAIKTLR
                     YVIDNQEAFHRKNSRYGTLLDLFGRTPLDVPHNGDSFQRRGYRFNLEVTGDGFKISAM
                     PVQPGPRPFLGDDSGIIRPGLE"
     gene            complement(559..1098)
                     /locus_tag="VEO00_03380"
     CDS             complement(559..1098)
                     /locus_tag="VEO00_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYU57077.1"
                     /translation="MKELHDILGAMTAQKDKVPDRKELLLDMSERVVKAVSAAFTCKA
                     DEVAILVLTSDGKHLRFIAPRKFTDLGTIPITKRDSIAVTVLGKKSGEAMNNVPLVKH
                     VSFFESVKLRDRVVPIQKMITVPILFRGQPLGVAQISRKGETPAEAGPDFTPADVRRA
                     QQIFEGVAPFFAEARPPKF"
     gene            complement(1167..2285)
                     /locus_tag="VEO00_03385"
     CDS             complement(1167..2285)
                     /locus_tag="VEO00_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYU57078.1"
                     /translation="MGSDQRGDVLVAGGRVVAAGGAADRRIELAGRWLLPGLVNALDV
                     LDLSVFPPLGMPPFTSLYDWGASLGPDAEAALRAALAVPLADRLFLGGLRNLLAGATA
                     VVHHHPYHRSLGREDFPVRVLCRYQFADSPGRTPALRRTYRSSDRRIPWLVRAAEGTD
                     DRARAELDVLAAANVLRQNTVILHGTGLPPEDGPRIAAARATVVWCPEADRRLYGTTA
                     PVTALWAAGVRVGLGSDSPATGSRDALSNLAAARREGVLDDAGLLTLATAESGAVGRL
                     PVGGLEEGFVADLVAVDDLDRFLGGDRRAIALVLVAGRPVYGDPELLAAAAIDSLALT
                     VDGSPRRLAAPHAARLRALLKAHPAVSAATWLRGVCLP"
     gene            complement(2319..>3305)
                     /locus_tag="VEO00_03390"
     CDS             complement(2319..>3305)
                     /locus_tag="VEO00_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYU57079.1"
                     /translation="WREGDHVVTELPRGPFDPTRWARADYGLLELDPYFRAQGARRLD
                     YCSSRWPWPALPGPAGEVAPLEWSALSPERVVAEMRELGLRLRPDEVAFQDDDLFADP
                     RRAEAIARGLLEAGVRIAWSASGRAAALARFGAESFRALAASGCVRVHVRTPGETPLP
                     GDAAEAILETARRLQAAGIGARFSFRAGFPGQGTDGLAAIHRLACTLRRMDARFETPI
                     RLHAPFPGGPGSNPAGFRVPERLEEWAEAGLEIGGPWIPAGLRRRAQRYHFFLSEAYR
                     PPARRWGKRLLRLVARGRVLLGFYGLDVERHAVEWSARLRTGRDRGPTLADD"
BASE COUNT          479 a         1220 c         1214 g          392 t
ORIGIN      
        1 cgccgacccg cagcgccgaa gggcccgctt ccggccgcaa gggcggtcct gcttccggct
       61 ccgcaggcca gaccggcggt acgacgtcac ccggaaccac ccccggcaac gagatggcat
      121 tcctcgacaa cgaagagccg atcgacaacg acgggcgggc ggccggcgaa gccctggccc
      181 agcgctacaa gagcgaccag ggcgggagcc gcgggagcac ctacggcacg acgggccgct
      241 tccggcagcg cgagcgcaac ccgcgcccca acagtccgaa cgagctcgcc gcgatcaaga
      301 ccctgcgcta cgtcatcgac aaccaggagg cgttccaccg caagaacagt cgctacggga
      361 cgctcctcga cctgttcggg cggactcccc tggacgtgcc ccacaacggc gactcgttcc
      421 agcggcgcgg ctaccggttc aacctcgagg tgacgggcga cggcttcaag atctcggcca
      481 tgccggtcca gccggggccg cgcccgttcc tgggagacga ctcgggaatc atccggccgg
      541 gcctggagta gccccggctc agaatttcgg gggccgcgcc tcggcgaaga agggggccac
      601 gccctcgaag atctgctggg cgcggcgcac gtcggcgggc gtgaagtcgg gtccggcctc
      661 cgccggggtc tcccccttgc ggctgatctg ggccaccccc agcggctgac cccggaacag
      721 gatggggacc gtgatcatct tctggatggg caccacgcgg tctcgcagct tcacgctctc
      781 gaagaaagag acgtgcttga ccagggggac gttgttcatg gcctcgccgg acttcttgcc
      841 gaggacggtc accgcgatgg agtcgcgctt cgtgatcggg atggtcccga ggtcggtgaa
      901 cttccggggg gcgatgaagc gcaggtgctt gccgtccgag gtgagaacca ggatcgcgac
      961 ctcgtccgcc ttgcaggtga aggccgcgct gaccgccttc accacccgct ctgacatgtc
     1021 gaggaggagc tccttccggt cggggacttt gtccttctgc gcggtcatcg ccccgaggat
     1081 gtcgtggagc tctttcatcc cttcccttcc ccggcccggc cgaaaaccgc gcgattatag
     1141 cacgcggcgt cgcgcgctcc ccttgctcac gggagacaca cgccgcgcag ccacgtggct
     1201 gcgctgactg cggggtgtgc cttcagcagg gcgcggagcc gggccgcgtg cggcgccgcg
     1261 agccggcgcg gcgacccgtc gaccgtgagc gcgaggctgt cgatcgcggc cgcggccagc
     1321 agctcgggat cgccgtacac gggccgcccc gccaccagga cgagggcgat cgcccggcgg
     1381 tcgcccccga gaaagcggtc cagatcgtcc accgccacga ggtcggccac gaatccctct
     1441 tccagaccgc ccaccgggag gcgtcccacg gccccgcttt ccgcggtggc cagggtgagg
     1501 aggcccgcgt catcgagcac gccctcgcgg cgggccgcgg ccaggttcga gagcgcgtcg
     1561 cggctccccg tggcggggct gtcgctgccg aggcccacgc ggacgccggc ggcccaaagg
     1621 gcggtgaccg gggcggtggt cccgtagagc cggcgatcgg cctccgggca ccacaccacc
     1681 gtggcgcggg cggcggcgat ccgcggaccg tcctcgggcg gaagcccggt gccatggagg
     1741 atcacggtgt tctgcctcag cacgttcgcc gccgccagca cgtccagctc cgcgcgggcg
     1801 cggtcgtcgg tcccctccgc cgcgcgcacc agccagggaa tccggcggtc gctcgagcgg
     1861 taggtgcgcc gcagcgcggg cgtccgcccc ggcgagtccg cgaactggta ccggcagaga
     1921 acgcggaccg ggaagtcttc gcggccgagc gagcggtgat acgggtggtg gtggacgacc
     1981 gcggtggctc ccgcgagcag gttgcgcaga cctccgagga agagccggtc ggccagggga
     2041 acggccaggg cggcgcgcag ggcggcctcg gcgtccggcc cgagcgacgc cccccagtcg
     2101 tagaggctcg tgaacggcgg catccccagc ggcgggaaga ccgagaggtc gagcacgtcg
     2161 agggcgttca cgagccccgg gagcagccag cgcccggcga gctcgatcct gcggtctgcc
     2221 gcgccgcccg cggcgacgac gcgtccgccc gccaccagga cgtcgccccg ctgatcgctc
     2281 cccacgcgtc gggcgtcccg gaggagcatc gcgccccgtc agtcgtccgc gagcgtgggg
     2341 ccgcggtcgc ggccggtgcg gaggcgcgcc gaccactcga ccgcgtgccg ctccacgtcg
     2401 agcccgtaga agccgaggag gacccgtccg cgagcaacca ggcgtagcag ccgcttgccc
     2461 cagcggcgtg cgggcggccg gtaagcctcg ctcaggaaga agtggtaacg ctgggcacgc
     2521 cggcgcagcc cggcggggat ccaggggccg ccgatctcga gtcccgcctc cgcccactcc
     2581 tcgagccgct cggggacgcg gaagccggcc gggttcgacc ccggcccccc tgggaagggg
     2641 gcgtggagac ggatcggggt ctcgaaacgc gcgtccatgc gccgtagcgt gcaggccagg
     2701 cggtgaatcg cggccagccc gtccgttccc tggccgggga agcccgcgcg gaaggagaaa
     2761 cgtgcgccga tgccggccgc ctgcagccgg cgggccgtct cgaggatcgc ctctgccgcg
     2821 tcgccgggaa gcggcgtctc tccgggtgtg cgcacatgga cgcggacgca gccgctcgcg
     2881 gcgagagcgc ggaaggactc cgcgccgaac cgggccaggg ccgcggcccg ccccgaggcc
     2941 gaccaggcga tgcgcacccc cgcctcgagg agcccgcgcg cgatggcctc ggcccgacgc
     3001 gggtcggcga acaggtcgtc gtcctggaag gccacctcgt ccggccgcag ccgcaacccg
     3061 agctcgcgca tctccgcgac cacgcgctcg ggcgagaggg cgctccactc gaggggcgcc
     3121 acttccccgg ccggcccggg aagcgccggc cagggccatc ggctcgagca gtagtcgagc
     3181 cgacgcgccc cctgagcccg gaaatagggg tcgagctcga gcaggccgta gtcggcgcgg
     3241 gcccagcgtg tcgggtcgaa ggggccgcgc ggcagctcgg tcaccacgtg gtccccctca
     3301 cgcca
//
LOCUS       DASPWO010000145         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_105044, whole genome shotgun sequence.
ACCESSION   DASPWO010000145 DASPWO010000000
VERSION     DASPWO010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105044"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VEO00_03395"
     CDS             complement(<1..219)
                     /locus_tag="VEO00_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HYU57080.1"
                     /translation="MLVLKEKADIVASNGIVFHSERMELPAPSVIRLRYFVNVPYRAR
                     AALTRRAVFARDGWVCQYCGAGAENLDHV"
     gene            651..1655
                     /locus_tag="VEO00_03400"
     CDS             651..1655
                     /locus_tag="VEO00_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYU57081.1"
                     /translation="MIDQPEPGGVVVARREPGDWERFASSFSAIANNVRQVIQGKERE
                     VRLALVCLIAEGHLLIEDVPGVGKTMLAKSMARSIDCSFRRIQFTPDLLPTDITGVNV
                     FNQERGDFEFKPGAVFANIVLGDEINRASPKTQSALLESMEERQVTVDGVTYHLGIPF
                     MVIATQNPIEHEGTYPLPEAQLDRFLMRLAIGYPGVDAEAGILATHGISSSLEEIGPV
                     TDARGVHAMIALTRDVHVADALRRYIVELAAATRGHPEIYLGASPRASIMLLRAARAM
                     AASNGRDYAIPDDVKTLAVPVLAHRVIVAADAVMSGRSAQAVLTELLREVEVPVAGHS
                     "
     gene            1665..>2259
                     /locus_tag="VEO00_03405"
     CDS             1665..>2259
                     /locus_tag="VEO00_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYU57082.1"
                     /translation="MRLTIRGWGTLGAGFALYVVSRMLGSPNLHMVAVGVMALPLLAL
                     AFLHFAPRRIHVKRRPAATRAFPGTRIRVDLSLTNGGRVWTPLILLEDTVPSALGRAA
                     RAVLPGIPGGNDQTVSYLVPCRSRGRYPLGPARLLLSDPWGLARARVEDPHRDELIVY
                     PEVEELAPWPLSSFGSGAGDSLAHQLFRSGEEFYGMRE"
BASE COUNT          329 a          815 c          767 g          348 t
ORIGIN      
        1 gacgtggtcg aggttctccg cccccgcccc gcagtactgg cacacccacc cgtcacgggc
       61 gaacaccgcg cggcgggtca gggcagcccg ggcccggtag ggcacgttga cgaaatagcg
      121 gagccggatc accgacggcg cgggcaactc catgcgctcg gaatggaaca cgatgccgtt
      181 ggacgcgacg atgtcagcct tctccttgag gacgagcacc accgcccttc gggccggcac
      241 gaccgcgagg ggttgttccg tcgcgttgag aacgagggat ctacccacct ctgacctgcg
      301 gcgagtataa caacggctcg ctcgcatccc ggtcgagcgg cgtgcttgac ttccgaagct
      361 cacacgcgtg taatttccgg cggggaagat ggagtcgcac tccgcctcag ggactgatgg
      421 cgcccgactg gatcgaacag aggtccagtc cacgacatag gggccgcgcg gggcgtcttc
      481 gagtacgagg ggcggggtgg caccgtcttc cctcttctgt tccgcccggc tcgcccccgg
      541 gctcccgatc ggaaacctcg agccctcgcc gtacgtcttc cgtcccggac gacgctggcg
      601 ggtgggcagg ggaaacttcc ccggccgaat caccgtactt ggaggataag atgatcgatc
      661 aaccagaacc cggaggggtc gtggtcgcgc gcagggagcc cggtgattgg gaacgcttcg
      721 cttcgtcctt ctcggccatc gcgaacaacg ttcgccaggt catccagggg aaggagcgtg
      781 aggtcaggct ggccctggtc tgcctgatcg ccgagggaca tctgctgatc gaggacgtcc
      841 cgggggtggg caagaccatg ctggccaagt ccatggcccg gtccatcgat tgctccttcc
      901 gccggatcca gttcacgccc gacctgctcc ccaccgacat caccggcgtg aacgtgttca
      961 accaggagcg cggcgacttc gagttcaagc cgggcgccgt gttcgccaac atcgtgctcg
     1021 gggacgagat caaccgggcc agcccgaaga cccaatcggc gctcctggag agcatggagg
     1081 agcgccaggt gaccgtggac ggcgtcacgt accacctggg catcccgttc atggtcatcg
     1141 ccacccagaa cccgatcgag cacgagggca cctacccgct gcccgaggcg cagctcgacc
     1201 ggttcctgat gcgcctggcc atcggctatc cgggggtcga cgccgaggcc ggcatcctgg
     1261 cgacccacgg gatcagctcc agcctcgagg agatcgggcc ggtcaccgac gcccgcgggg
     1321 tccacgccat gatcgccctg acccgcgacg tccacgtggc cgacgcgctg cgccgctaca
     1381 tcgtcgagct ggcggcggcg acccgcggtc atccggagat ctacctcggc gcaagccccc
     1441 gcgcctcgat catgctgctc cgggcggccc gggcgatggc cgcgagcaac ggtcgcgact
     1501 acgccatccc cgacgacgtg aagacgctcg ccgtcccggt gctcgcccac cgggtcatcg
     1561 tcgccgccga cgcggtgatg tccgggcgct cggcgcaggc ggtcctcacg gagctgctgc
     1621 gcgaggtcga ggttcccgtc gcggggcact cctagggggc cccggtgcgg ctgaccatcc
     1681 gcggctgggg gacgctcggc gccgggttcg ccctctacgt cgtctcgcgc atgctcggct
     1741 cccccaacct gcacatggtc gccgtcgggg tgatggccct tcccctgctg gcgctggcct
     1801 tcctgcactt cgccccgcgc cgcatccacg tcaagcgccg gccggcggcg acgcgcgcgt
     1861 tccccgggac ccgtatccgg gtggacctct ccctgaccaa cgggggccgg gtctggacgc
     1921 cgctgatcct gctggaggac acggtcccgt cggccctggg gcgagcggcg cgggcggtgc
     1981 tgccgggtat cccgggcggc aacgaccaga ccgtcagcta cctggtcccc tgccgcagcc
     2041 gcggccgcta cccgctgggc ccggcccggc tgctgctctc cgatccgtgg gggctggccc
     2101 gggcgcgcgt ggaggacccc catcgcgacg agctgatcgt ctaccccgag gtcgaggagc
     2161 tcgcgccgtg gccgttgtcg tcgttcggct ccggcgccgg cgactccctc gcccaccagc
     2221 tctttcggag cggcgaggag ttctacggca tgcgcgagt
//
LOCUS       DASPWO010000146         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_444481, whole genome shotgun sequence.
ACCESSION   DASPWO010000146 DASPWO010000000
VERSION     DASPWO010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444481"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VEO00_03410"
     CDS             complement(<1..618)
                     /locus_tag="VEO00_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU57083.1"
                     /translation="MDFEFSSRSLELQERLGDFITTHVDPAGPVYQEQVAASGDPHFH
                     PPVMEELKAEARKRGLWNLFLPNEKWGAGLSNLDYAPLCEMMGKSLLTGEATNCAAPD
                     TGNMEILAEFGTAEQQDEWLVPLLEGRIRSCFAMTEPWVASSDATNIQSRIEPDGDHY
                     IVNAHKWWTSGAASDRCKVAVLLGVSDPDADPYHRHSMILVPFDAP"
     gene            675..1778
                     /locus_tag="VEO00_03415"
     CDS             675..1778
                     /locus_tag="VEO00_03415"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYU57084.1"
                     /translation="MEPDVGTALLAATWIVGWLLFFRFPSLPVARPARDRRVSVVIPA
                     RDEAAVLPRLLAALDRQTMAAEVVVVDDHSADDTGAVAFTAGAQVLPAPPLPDGWTGK
                     ASACWYGAHQTTGEVLVFLDADTEPAPDLLVRLVAELDRRGGLVSVAPYHRMARVYER
                     LSAVFHAIAVMGIGAASARPGAPVTGAFGPCLACTRADYADVGGHDVVRSAVADDIAL
                     ARAFRRTGLPVHVVAGREAIGYRLYPNGARQLADGWSKNFAAGARSTPLVRFLLVMVW
                     VIGLGTAAQVPFRSVLNALAGWSYPGVIGWVAYAAFATQLAVMLRPLGNYSWAAPLFP
                     VPLAAWFVVFARSIVWMVRGTVRWKGRVVPVRP"
     gene            1775..>2046
                     /locus_tag="VEO00_03420"
     CDS             1775..>2046
                     /locus_tag="VEO00_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57085.1"
                     /translation="MTGTVFERRNVWLAALDAGVWAAWSALAGYVAHRLSVERLGRDG
                     WLFRLRRAPERVARRYERRLRIKRWKSLLPEAGDVFRGGFSKRRLLR"
BASE COUNT          230 a          750 c          721 g          345 t
ORIGIN      
        1 gggcgcgtcg aacggcacga ggatcatcga gtgccggtgg tacgggtcgg cgtcggggtc
       61 cgacacaccc aggagcacgg cgaccttgca ccggtcggat gccgcgccgc tcgtccacca
      121 cttgtgcgcg ttcacgatgt agtggtcgcc gtcgggctcg atgcggctct ggatgttggt
      181 cgcgtccgag ctcgccaccc acggttcggt catcgcgaag cacgagcgga tccggccttc
      241 gagcagcggg acgagccact cgtcctgctg ctcggccgtc ccgaactcgg ccaggatctc
      301 catgttgccg gtgtcgggcg cggcgcagtt cgtcgcctct cccgtcagca ggctcttgcc
      361 catcatctcg cacagcggcg cgtagtcgag gttggagagg cccgcgcccc atttctcgtt
      421 ggggaggaag aggttccaca ggccgcgctt gcgggcttcg gccttgagct cctccatgac
      481 cggcgggtgg aagtggggat cacccgacgc cgccacctgc tcctggtaca cgggcccggc
      541 cgggtcgacg tgcgttgtga tgaagtcgcc gagcctctcc tgaagctcga gcgaccggga
      601 gctgaattcg aagtccatat cgcccgaacc ctacttccgg catccggaca cggctccggc
      661 gtagaaatac ccctgtggag cctgacgtcg gaactgcgtt gctggcggcg acgtggatcg
      721 tgggctggtt gctgttcttc cggttcccgt ccctccccgt cgcccgtccg gcgcgcgacc
      781 ggcgggtctc ggtggtgatc cccgcccgcg acgaggccgc ggtgctgccg cgcctgctcg
      841 ccgccctcga ccgccagacc atggcggcgg aggttgtcgt cgtcgacgac cactccgccg
      901 acgacaccgg cgcggtcgcg ttcaccgccg gcgcccaggt cctgcccgcc ccgcccctcc
      961 ccgacggctg gaccggcaag gcatcggcgt gctggtacgg cgcgcaccag accaccggcg
     1021 aggtgctggt gttcctcgac gccgacaccg agcccgcgcc cgacctcctg gtccggctgg
     1081 tcgccgagct cgaccgccgc ggcggactcg tctcggtggc gccctaccac cgcatggccc
     1141 gcgtgtacga gcggctgtcg gcggtcttcc acgcgatcgc ggtcatgggg atcggcgcgg
     1201 cctcggctcg tcccggcgcg ccggtgaccg gcgcgttcgg tccctgcctg gcctgcaccc
     1261 gcgccgacta cgccgacgtc ggtggccacg acgtcgtccg cagcgcggtc gccgacgaca
     1321 tcgcgctcgc ccgcgccttc cgccgtaccg gcctgccggt gcacgtcgtc gccggtcgtg
     1381 aggcgatcgg gtaccgcctc tacccgaacg gcgcccgtca actcgccgac gggtggagca
     1441 agaacttcgc cgccggggcg aggtcgactc cgctcgtgcg cttcctgctc gtcatggtgt
     1501 gggtgatcgg tctcgggacc gccgcccagg tgccgttccg ttccgtgctc aacgcgctgg
     1561 ccgggtggtc gtaccccgga gtcatcggct gggtcgccta cgccgccttc gccacccagc
     1621 tcgcggtcat gctgcggccg ctcggcaact actcgtgggc cgcgccgctg ttcccggtgc
     1681 ccctggcagc gtggttcgtc gtcttcgcgc ggtcgatcgt ctggatggtg cgcggcaccg
     1741 tgcgctggaa gggccgggtc gtgcccgtcc gaccgtgacc gggaccgtct ttgaacgccg
     1801 caacgtgtgg ctcgccgccc tcgacgccgg cgtctgggcg gcgtggagcg cgctcgccgg
     1861 ctacgtggcc caccggctgt cggtggagcg gctgggtcgg gacggctggc tgttccggct
     1921 ccgccgcgcg cccgagcgcg tggcccggcg gtacgagcgg cggctgcgca tcaaacgatg
     1981 gaaatcgctg ctgcccgagg cgggcgatgt cttccgcggc ggcttctcga agcgacgcct
     2041 gctgcg
//
LOCUS       DASPWO010000147         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_279057, whole genome shotgun sequence.
ACCESSION   DASPWO010000147 DASPWO010000000
VERSION     DASPWO010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279057"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(610..1419)
                     /locus_tag="VEO00_03425"
     CDS             complement(610..1419)
                     /locus_tag="VEO00_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57086.1"
                     /translation="MRFDERARRATQGIHRAVEVMEMSNTKTPQRLTRFDQYRARMSR
                     NQRIVAIAVGVGLPLLLLVGAVRLLGSDPDPTVPLAPSSTSVSPTPVRGRTALFEAPF
                     TYTLPPGWTVSEELRAVELRPPEAPEGWRDFWVLGSLVPARSDCSPRPERGDGRSSDA
                     MTSWLSTHPALDTTTPREVTLGGATGSWVDVQLADDADQTCLAGFTLLTGDPDWEGRW
                     SIGAGEKIRFYVLDVPAGGTVTIAINPINATYFNDVIDLAAPVVESFKFLD"
     gene            complement(1416..1967)
                     /locus_tag="VEO00_03430"
     CDS             complement(1416..1967)
                     /locus_tag="VEO00_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HYU57087.1"
                     /translation="MTWTYVEAGDGVSHGGEPAVYSSETFEEFYAREFPAIVALAYAL
                     SGSRTASEDLAQEALLAAHCDWDRVGGFERPDVWVRRVVTNLSMSMFRHRYAEAKALA
                     RLALGTATTMPALGAEDAEFWAAVRSLPRRQAQVIALRYLDDRSVAEIAEILGTATGT
                     VKKHLHDGRRAVAHRLRLEEDDG"
BASE COUNT          312 a          775 c          714 g          324 t
ORIGIN      
        1 cacctcagcc gctccccccc ggcccccact gtgatcacgc gcacgatgcc ccgctcgccg
       61 catcgatcgg cgcgattatg aggcacgaat gacggccaga agggcgggga ctgctggtgt
      121 cctcctggag tcgacgccgg ctatcacatc ttgtgacctc tctatgcccg aagctacggg
      181 cgcgggggcc ggtctctcca catcggccac ctggctacga ctggcagacg acgcggcggg
      241 gtcggcggac ctccgagcgc ggctggaaca tcgccggaga gttcgtcgac gcccgcgagt
      301 ccgcgcgccg gccagccgcg atcgcgctgc tgtccggggg ctcgtgagcg gaccgtcccc
      361 gacctttcgg tgggagcgcc gcggtcgtga acggggacgg tcaacttcag gtaccgcgac
      421 agcggcgcgt taggggccgc ctcggccctt gtcttcccag gggccgcccc ctgaaccgtg
      481 cccggcgccg gcccacggcg ccgggcctca ccgaggaacg acatcggcgc ggcggcgtcg
      541 cccctcaccc cggcctgcca gacgccgccc ggggggcatc ctcgagatgc cctccgggcg
      601 gtcctccggt cagtcgagga acttgaacga ctccacgacc ggcgcagcca ggtcgatcac
      661 atcgttgaag taggtcgcgt tgatgggatt gatggcgatc gtgaccgtcc cgccggcggg
      721 aacgtcgagc acgtagaaac gaatcttctc gcccgcgccg atcgaccacc tcccctccca
      781 gtcggggtca cccgtgagga gcgtgaaccc ggccaggcag gtctgatccg cgtcatcggc
      841 gagctggacg tccacccagc ttcccgtggc cccgccgagc gttacctctc ggggtgtcgt
      901 ggtgtcgagc gccgggtggg tcgatagcca gctcgtcatc gcgtcgctcg accggccgtc
      961 accccgctcc ggccgagggg aacagtcaga ccttgccggc acgaggctgc caaggaccca
     1021 gaagtccctc cacccctccg gcgcttcggg cgggcgcagc tcgaccgccc ggagttcttc
     1081 cgagaccgtc cagccggggg gcagcgtgta cgtgaatggc gcctcgaaca acgcggtgcg
     1141 gccgcgcacc ggtgtcggcg acaccgaggt cgacgacggc gcgaggggga ccgtcggatc
     1201 ggggtccgac cccagcaagc gcacggcgcc gaccaagagc aggagcggca gccccacccc
     1261 cacggcgatc gccacgatcc gttggttcct cgacatcctc gcgcggtact gatcgaagcg
     1321 cgtgagcctc tgcggtgtct tggtgttcga catctccatc acctccacgg cgcggtggat
     1381 gccctgcgtt gcccgtcgag cacgctcgtc gaatctcatc cgtcgtcctc ctcgagcctc
     1441 aggcggtggg ccacggcccg ccgcccgtcg tgcaggtgct tcttcaccgt gccggtcgcg
     1501 gtgccgagaa tctcggcgat ctcggcgacc gatcgatcgt ccaggtaacg gagcgcgatg
     1561 acctgcgcct gtcgtcgcgg aagggaccgg acggccgccc agaactcggc gtcctcggca
     1621 cccagtgccg gcatcgtggt cgcggttccc aacgcgagcc gcgccagcgc cttcgcctcg
     1681 gcgtagcgat ggcggaacat cgacatcgag aggttcgtca cgacccggcg cacccagacg
     1741 tcggggcgtt cgaacccgcc cacgcggtcc cagtcgcagt gggcggccag gagggcctcc
     1801 tgggcgaggt cctccgatgc ggtgcggctc cccgagagcg cgtacgcgag cgcgacgatc
     1861 gccgggaact cccgcgcgta gaactcctcg aacgtctccg aggagtacac ggcaggctca
     1921 cccccgtgcg atacgccgtc gcccgcttcc acgtacgtcc aggtcatcgt cgctccaccc
     1981 cctccatcgc gcgaggggcc ttggaggttt agtcggattg tcgtcggagc atggagggat
     2041 gggcggccat gtacccttct cttcgcaatc gtgacgggtc gccctgcggt accccacctg
     2101 ggcgaatcgg cctggaccta taggg
//
LOCUS       DASPWO010000148         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_954435, whole genome shotgun sequence.
ACCESSION   DASPWO010000148 DASPWO010000000
VERSION     DASPWO010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954435"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..994
                     /gene="hemB"
                     /locus_tag="VEO00_03435"
     CDS             17..994
                     /gene="hemB"
                     /locus_tag="VEO00_03435"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HYU57088.1"
                     /translation="MSFPANRPRRLRRTEALRRLVRETDLSPRHLVAPLFLKESLTEP
                     APIGSMPGQAQHTPESLRKEARAIAGRGVGAFVLFGVPARKDADGSEAWNPDGVAQQG
                     LRALREELGDEAVLIADLCLDEYTDHGHCGVLDDAGEVDNDATLERYARIAVTQAGAG
                     ADLVAPSGMMDGQVGAIRAALDGKGYATTGILAYAAKFASSFYGPFRDAAEGAPRFGD
                     RRGYQMDPANGDEALREIEADLEEGADIVMVKPALPYLDVVARARDAFGGPIAAYNVS
                     GEYAMVKAAAERGWVDERAAVLEILTGIRRAGADLILTYHARDAADWLS"
     gene            complement(1597..2007)
                     /locus_tag="VEO00_03440"
     CDS             complement(1597..2007)
                     /locus_tag="VEO00_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57089.1"
                     /translation="MRTWFALPAALAVALLTAAPASAHGTHTRPAITRLEWLTGPVAV
                     GEEEFLVVEAHDPDSSISEVDVVWGDEGISFAHTYCVQGTEIGKPARLVLSWKFPHPG
                     LEDVRVVALSQDHCKPNRSIQRSPTKHLPTPVRP"
     gene            complement(2085..2519)
                     /locus_tag="VEO00_03445"
     CDS             complement(2085..2519)
                     /locus_tag="VEO00_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HYU57090.1"
                     /translation="MSLRFVRGEPDRHPVNIRHFGKDRTPGERVADLVAARVGSWPFI
                     IIQSTLLALWIVANALILGDWIGGKPFDPYPFILLNLTLSFQAAYTGPIVMMSQNRQA
                     AKDRDEAEHDYQVNQEALDRLKALQESHDRLLAAVEELRAAH"
     gene            2628..>2914
                     /locus_tag="VEO00_03450"
     CDS             2628..>2914
                     /locus_tag="VEO00_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57091.1"
                     /translation="MSAPDTLTIEDLAEDTGEIVADLRVWHELGLLPGDPGAFPGSHL
                     ARVGLIPFASRRGFEPARLAEFYGSELTGATLAAAAPLRGLLEPAVRYFHR"
BASE COUNT          403 a         1027 c         1063 g          421 t
ORIGIN      
        1 tagcatcgtc ggcacgatga gcttcccggc caaccggccc cgccgcctgc gccgcaccga
       61 ggcgttgcgg cggttggtgc gcgagaccga cctctcaccg cggcacctgg tcgcaccgct
      121 gttcctgaag gagagcctca ccgagccggc gccgatcggc tcgatgccgg ggcaggccca
      181 gcacacgccc gagtcgctgc ggaaggaggc gcgggccatc gccgggcgcg gcgtcggggc
      241 cttcgtgctc ttcggcgtgc cggcgcgcaa ggacgccgac ggctccgagg cctggaatcc
      301 cgacggggtg gcccagcagg ggctgcgcgc gctgcgcgag gagctcggag acgaggcggt
      361 gctgatcgcc gacctgtgcc tggacgagta caccgaccac ggacactgcg gggtcctgga
      421 cgacgccggc gaggtggaca acgacgccac gctcgagcgg tacgcgcgca tcgcggtcac
      481 tcaggccggc gcgggcgcgg acctggtggc cccgtcgggg atgatggacg ggcaggtcgg
      541 ggcgatccgc gcggccctcg acggcaaggg gtacgcgacg acgggcatcc tggcctacgc
      601 cgcgaagttc gcgtcgtcgt tctacggccc gttccgggat gccgcggagg gcgcgccgcg
      661 attcggcgac cgccgcggct accagatgga cccggcgaac ggcgacgagg cgctgcgcga
      721 gatcgaggcg gacctcgagg agggcgcgga catcgtgatg gtcaagccgg cgctgccgta
      781 cctcgacgtg gtcgcccgtg cgcgcgatgc gttcggcggc ccgatcgccg cctacaacgt
      841 ctccggcgag tacgcgatgg tcaaggccgc ggcggagcgc ggctgggtcg acgagcgcgc
      901 cgccgtcctg gagatcctga ccgggatccg ccgcgccggc gccgacctga tcctcaccta
      961 ccacgcccgc gacgccgccg actggctgtc ctgacaacga cgcatcgcgc gagcatcagc
     1021 ggcaggctct tcggggagcc cacccgaatt gcggctgacc caaagtcagg cgacgttccg
     1081 ttcccgaggt tcggctgggt cagggatggg cgaatgcgag ctaccctccc tcaactgtct
     1141 gtccaggcgt cgaagcgggt gactacgtca agttcaagag gtgacgtcct acccttcctg
     1201 taaatgcgga gcggcctgag ccatcctcgt gatcgacgac caccgaggtg tcgagcagcc
     1261 cccggagcgc ctcaggcacc ggcggacggt tccacgaccg cgttcaggtc cccgcggagc
     1321 cgggctgcgt cggcgagggg cccgccgggc ggcgtcggcg aggacggccc gaggaacgaa
     1381 gcggcgcggg gggacggggc gcaactcggc gaccggcatc cggttgcggg tcacgacgat
     1441 cgtgtggccg gcctggacct cgcgaagcac ggccgccgag tcgttccgca gctaccgctg
     1501 actgatgctc ctcgtcatcc tcgcgcacgt cgcacatgtc gcgtttcgta gcaccgtgac
     1561 ggatcgcgtc gtgttctcca ccccgtgggc aggtgtctat ggcctcaccg gggtggggag
     1621 gtgcttcgtc ggggagcgct ggatcgaacg gttcggcttg cagtggtcct gggagagggc
     1681 gacgacccgg acgtcctcca gacccggatg cgggaatttc cacgagagca ccagccgggc
     1741 cggcttcccg atctccgtcc cctgcacgca gtaggtgtgg gcgaacgaga tcccctcgtc
     1801 tccccagacc acgtcgacct cactgatcga cgagtcggga tcgtgtgcct cgacgaccag
     1861 gaactcctcc tcgccgacgg cgaccggtcc ggtcagccac tcgagccggg tgatcgccgg
     1921 ccgggtgtgc gtcccgtggg ccgaggccgg cgccgcagtg agcagcgcga ccgccagcgc
     1981 ggccgggagc gcgaaccacg tccgcatgcg tcctcctctc gccgggttca cgcttcgacg
     2041 ccgccggggg tcgcccggtt ccccccgccg cgtccttgtg gtcgtcagtg ggcggcgcgc
     2101 agttcttcga cggctgcgag caggcggtcg tggctctcct ggagcgcctt cagccggtcg
     2161 agcgcctcct ggttgacctg gtagtcgtgc tcggcctcgt cgcggtcctt cgccgcctgg
     2221 cggttctggc tcatcatcac gatcgggccg gtgtaggccg cctggaacga gagcgtcagg
     2281 ttcagcagga tgaacgggta ggggtcgaac ggcttcccgc cgatccagtc gccgaggatc
     2341 agcgcgttcg ccacgatcca gagcgccagc agcgtcgact ggatgatgat gaagggccac
     2401 gaccccaccc gcgcggcgac caggtcggcg acccgctcgc ccggcgtgcg gtccttcccg
     2461 aagtgccgga tgttcaccgg gtgccggtcc ggctcaccgc ggacgaatct gaggctcatc
     2521 ggcgtccctc ccaactcgtc tggacgggcc gacgatatcc gccgcggagg cacgccacgg
     2581 cggattcgcg ccgggacggg ggagggggcg atgctgagcc cagcccgatg agcgcgcccg
     2641 acacgctgac gatcgaggac ctggcggagg acacgggcga gatcgtcgcg gacctccgcg
     2701 tctggcacga gctggggctg ctccccggcg atcccggcgc cttccccggg tcgcatctcg
     2761 cgcgggtggg gctgatcccg ttcgcctccc ggcgcggctt cgaacccgcg cggctggcgg
     2821 agttctacgg ctccgagctg accggtgcca ccctggccgc cgcggcgccg ctccgcggcc
     2881 tgctggagcc ggcggtgcgg tacttccacc gcaa
//
LOCUS       DASPWO010000149         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_701513, whole genome shotgun sequence.
ACCESSION   DASPWO010000149 DASPWO010000000
VERSION     DASPWO010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701513"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VEO00_03455"
     CDS             <1..1113
                     /locus_tag="VEO00_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HYU57092.1"
                     /translation="ERLPHPSGVRPRVSGKDVLRASVEGAAFAVDRWGVDPAELSRVE
                     ESGRVPVEPHGGPDGVRRELPWLVRQLSRLQFGTVGPTNHFVELQRVEEVFDPVAAGL
                     LGVAPGQLTIQYHAGGGVLTGIIGRMYGRRTDYPRKHRAFMAWQKPLVHLARARSLEE
                     FRRRMALYFTDGCPPVPREGTEGRRLLLANAAAMNYGFAYRLAVYAELRAVAAAALGA
                     AGSGLVVDSPHDTLYEEQHGGESVLVHRYKSCRAFPASKMAGHPVFGRTGQPVLVPGT
                     NRTSSFLCVADEGARSSLYSACHGTGTIVSAFAERGLSGQDPRGRKTLVYRYSDGAPA
                     EVPQLDDRGIDEAVGILHGHSIVRPVARLRPLAVLT"
     gene            1211..>2023
                     /locus_tag="VEO00_03460"
     CDS             1211..>2023
                     /locus_tag="VEO00_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HYU57093.1"
                     /translation="MSALARLAEKRRNPFAANLIARVAALGSLATVSLVVARSGGPAA
                     VGELALLRVLPWLTGVILTCGLYGAAPYFLAGRAGPDPRYRPTIVGIALAAGAVGSAL
                     WFAGAGEIKEQFFHTVPIAIVTWAGVTVLTQVLESTAKACSQGMDDLPGANRVIVLEE
                     FMFLPMYLVFRAAGVEQSTSMLAGLALGDLFTLTPAWIRLYRRGFFHKAGRPSLALAR
                     RVCKFGMKAQAGTLLTLVNERLDFAIVGALVGPAALGIYAIASRFAELLRLPS"
BASE COUNT          227 a          751 c          754 g          291 t
ORIGIN      
        1 gagcggctgc cccacccgag cggcgtccgg ccccgggtct cggggaagga cgtcctgcgg
       61 gcatcggtcg agggcgccgc gttcgcggtg gaccggtggg gcgtcgaccc cgccgagctg
      121 tcacgcgtcg aggagtccgg gcgtgtcccc gtggagccgc acggcggccc ggacggggtt
      181 cgccgagagc tcccctggct ggtgcggcag ctctcgcggt tgcagttcgg cacggtcggg
      241 cccaccaacc acttcgtcga gctccagcgg gtggaggagg tcttcgaccc cgtcgccgcc
      301 gggctcctcg gcgtggcgcc cgggcagctg accatccagt accacgccgg gggcggggtg
      361 ctcaccggga tcatcggccg catgtacggg cgccgcaccg actacccgcg caagcaccgc
      421 gcgttcatgg cctggcagaa gcccctggtg cacctggccc gggcgcggtc cctggaggag
      481 ttccggcgtc gcatggcgct gtacttcacc gacggctgcc ccccggtccc ccgggagggg
      541 acggagggcc ggcggctgct gctggcgaac gccgccgcga tgaactacgg gttcgcgtac
      601 cggctggccg tgtacgccga gcttcgcgcc gtcgcggccg cggccctggg ggccgccggg
      661 agcgggctgg tggtggactc cccgcacgac acgctgtacg aggagcagca cggcggcgag
      721 tcggtgctcg tgcaccgcta caagtcctgc cgggccttcc cggcctccaa gatggccggc
      781 catccggtgt tcgggcggac cggccagccg gtgctcgtgc cggggacgaa ccggacgtcg
      841 tcgttcctgt gcgtggccga cgagggcgca cggtccagcc tgtactcggc gtgccacggg
      901 acggggacca tcgtctcggc cttcgccgag cggggcctgt ccggtcagga cccccgcggc
      961 cggaagaccc tcgtctaccg atactccgac ggcgcgccgg ccgaggtccc gcagctcgac
     1021 gaccgcggca tcgacgaggc cgtcggcatc ctgcacggcc acagcatcgt gcgccccgtg
     1081 gcccgcctcc gacccctggc ggttctgaca tgagccgggc ggccgatcgg aacgaagccg
     1141 tggggcgggg ctcgtccccg ccccgggccg ggcgcaaggc ccggcccaca cggacgaggg
     1201 gtgtgaccac gtgagcgcgc tggcccgtct cgcggagaag cggcgaaacc cgttcgcggc
     1261 gaacctcatc gcgcgggtgg cggcgctggg ctcgctggcg acggtcagcc tggtggtggc
     1321 ccgctccggc ggcccggccg cggtcggcga gctggccctg ctgcgcgtcc tcccctggct
     1381 gaccggggtg atcctgacgt gcgggctgta cggcgccgcc ccgtacttcc tggccggccg
     1441 ggccgggccc gacccgcgct accgccccac catcgtcggc atcgcgctcg cggccggtgc
     1501 ggtcggctcg gccctgtggt tcgcgggcgc cggcgagatc aaggagcagt tcttccacac
     1561 cgtccccatc gccatcgtca cgtgggccgg cgtcacggtc ctgacccagg tcctggagtc
     1621 aacggcgaag gcgtgctcgc agggcatgga cgacctcccc ggcgcgaacc gggtgatcgt
     1681 gctcgaggag ttcatgttcc tgcccatgta cctggtcttc cgcgcggccg gcgtcgagca
     1741 gtccacgtcc atgctggccg ggctggccct gggcgacctg ttcaccctga ccccggcgtg
     1801 gatccgcctg taccgccggg ggttcttcca caaggccggc cggccctcgc tggcgctggc
     1861 ccggcgcgtg tgcaagttcg ggatgaaggc ccaggccggg acgctgctga ccctggtgaa
     1921 cgagcgcctg gacttcgcca tcgtcggcgc cctggtgggg ccggcggcgc tcggcatcta
     1981 cgccatcgcc tcgcgcttcg cggagctgct ccgcctgccc tcg
//
LOCUS       DASPWO010000150         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_445030, whole genome shotgun sequence.
ACCESSION   DASPWO010000150 DASPWO010000000
VERSION     DASPWO010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445030"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VEO00_03465"
     CDS             complement(<1..433)
                     /locus_tag="VEO00_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57094.1"
                     /translation="MSGDRPVAGVPDHDRIDELLAGHALGALSGEDLAAAEALFAEHV
                     PGCPDCRRTIGELEALTGDLGLAAPTRPAPDTLWASLRREIRRPLPITAPRRLRLPTW
                     AAAAAAVAVVALGGLSATLSSRAAQAESQRAGLVDMLGFVGQ"
     gene            complement(430..996)
                     /locus_tag="VEO00_03470"
     CDS             complement(430..996)
                     /locus_tag="VEO00_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU57095.1"
                     /translation="MDPAEDRHDIELVRRVALRDRDAFEQLYKRYQASAFGLALRITG
                     QELLAQEVAHEAFLAVWRAPEAYDPARGAFRTFFLALVHHRAVDTVRREDRLRRRQER
                     VNRHVEIGEDVADGVVEEAWLDVRRKQVRQALTALPEEQRQVLELAYFQGMTQARIAE
                     VLAIPLGTVKTRTLAAMRKLRAILAEDA"
     gene            1037..1279
                     /locus_tag="VEO00_03475"
     CDS             1037..1279
                     /locus_tag="VEO00_03475"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HYU57096.1"
                     /translation="MPPAHRVRMVSRATCGLCDRAREAILALRATLDFAYEEVFIDGD
                     DALERAYGLRVPVVLVDGREQFESEVDGARLRDLLR"
     gene            1346..1972
                     /locus_tag="VEO00_03480"
                     /pseudo
     CDS             1346..1972
                     /locus_tag="VEO00_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
     gene            2034..>2222
                     /locus_tag="VEO00_03485"
     CDS             2034..>2222
                     /locus_tag="VEO00_03485"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HYU57097.1"
                     /translation="MPLLALGVSHRRAAADLLGRLAFGDDDVPKAYRRLAELPSVAGA
                     VLLSTCNRVEVYAEVGGYH"
BASE COUNT          281 a          807 c          823 g          311 t
ORIGIN      
        1 cctggccgac gaacccgagc atgtccacca ggccggcgcg ctggctctcg gcctgggccg
       61 cccgcgatga cagcgtggcc gacagcccgc cgagcgcgac caccgcgacg gccgccgcgg
      121 ccgccgccca ggtcgggagg cgcaggcggc gcggggcggt gatcgggagg gggcgccgga
      181 tctcgcgccg cagggaggcc cagagtgtgt ccggcgccgg ccgcgtcggc gcggcgagtc
      241 cgaggtcgcc ggtcagggcc tccagctcgc cgatcgtccg ccggcagtcg gggcagccgg
      301 ggacgtgctc cgcgaacagc gcttcggcgg cggccaggtc ctcgcccgac agcgccccga
      361 gggcgtggcc ggcgagcagc tcgtcgatgc gatcgtgatc gggcaccccg gccaccggcc
      421 ggtccccgct cacgcgtcct ccgcgaggat cgcccggagc ttgcgcatcg ccgccagcgt
      481 gcgggtcttg acggtgccga gcgggatcgc cagcacctcg gcgatccgtg cctgggtcat
      541 gccctggaag taggccaatt cgagcacctg tcgctgttcc tcggggagcg ccgtgagagc
      601 ctgccggacc tgcttgcgcc gcacgtcgag ccacgcctcc tcgaccacgc cgtccgctac
      661 atcttcgccg atctccacgt ggcggttcac ccgctcctgc cgccggcgga ggcggtcctc
      721 gcggcgcacg gtgtcgaccg cgcggtggtg caccagggcg aggaagaagg tccggaacgc
      781 gccgcgggcc gggtcgtacg cctcgggggc gcgccagacc gcgaggaacg cctcgtgcgc
      841 cacctcctgc gccagcagct cctggccggt gatccgcagg gccagaccga aggcggaggc
      901 ctggtagcgc ttgtagagct gctcgaaggc gtcgcggtcg cgcagggcga cccggcggac
      961 cagctcgatg tcgtgacgat cctcggccgg gtccacgggg agtgtctacc agatgccgcg
     1021 gtaggctccg aacccgatgc ctcccgcgca ccgcgtccgg atggtctcgc gcgcgacctg
     1081 cggcctgtgc gaccgcgccc gcgaggcgat cctggcgctc cgggcgacgc tcgacttcgc
     1141 gtacgaagag gtcttcatcg acggcgacga cgccctcgag cgcgcctatg gcctgcgggt
     1201 gcccgtggtc ctcgtcgacg gccgggagca gttcgagtcc gaggtcgacg gtgcccgcct
     1261 gcgtgacctt ctgcggtaag tgcatttgag aatcgattca cgagcgcact agaatcccgt
     1321 ccgtgcgacc cctccagggc ccctcgtgag ggagcgcccg atccccgaag cgacggtcgg
     1381 gcggctgccc gtctaccttc gcgcgctggt cgagatggcc gagaacgcca cctcgacggt
     1441 ttcctccgag acgctggccg cggcggccgg ggtgaactcg gcgaaggtgc gcaaggacct
     1501 ctcctacctc ggctcctacg gcacgcgcgg ggtcggctac gacgtggcct acctgatcca
     1561 ccagatccgc cgcgttctcg ggctgaccca gcactggccg gtgctgatcg cgggcatcgg
     1621 gaatctcggg cacgccctgg ccgggtaccg cgggttcgcc gagcgagggt tccgggtggg
     1681 ggcgctggtg gactcggacc cgagccgggt cggcgaggtg gtggacggcg ttcccgtgca
     1741 ccacgtgaac gacatcgagc gcctggtcaa ggagcgcgac atcgcgatcg gcgtgatctg
     1801 cacccccgcc gcggccgcac aggaggtggc cgacgcgatg gccgcggccg ggatccgctc
     1861 gatcctgaac ttcgccccgg cggtgatcac ggtcccggcc ggcgtctcgc tgcgcaaggt
     1921 cgacctgtcg atcgagctgc agatcctggc cttctacgag cagcgcaagg ccgcgctccg
     1981 agacgtgcgc ggcggccggg ccgtccccga cctgtcggcc gtacgctagg cggatgccgc
     2041 tcctggccct gggggtctcg caccgccgcg ccgcggccga cctgctgggg cgcttggcgt
     2101 tcggggacga cgacgtcccg aaggcgtacc ggcggctcgc cgagctgccg tcggtggccg
     2161 gggcggtgct gctgtccacc tgcaaccgcg tcgaggtcta cgcggaggtc ggcggctatc
     2221 ac
//
LOCUS       DASPWO010000151         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_445096, whole genome shotgun sequence.
ACCESSION   DASPWO010000151 DASPWO010000000
VERSION     DASPWO010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445096"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1582
                     /locus_tag="VEO00_03490"
     CDS             167..1582
                     /locus_tag="VEO00_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018837999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HYU57098.1"
                     /translation="MAGSRTNSHLANDLLVALRRDAQVPLHRQIEASIRDGIRSGRLR
                     LGTSLPPTRTIAADLGVSRGVVVEAYQQLLAEGYLTSRMGGYTQVAIGPEPAPTPPRP
                     VPARAARIDFGYGRADVSQFPRAAWLRSLRRVLAEAPSRRFGYPSGRGVPELHEALAD
                     YLNRVRGTSAKPDNVVICNGYAQGITLLIQVLAKSGAKRLAVEDPSSDDDARPIAVSA
                     GLDVVGIPVGHDGIRVDVLDRTDADAVVLTPSHQWPTGAVLPAASRAAVLAWAKRRGA
                     LIVEDDYDAEYRYDRAPIGAMQGLAPDHVVYAGSASKTLAPGLRLGWLVAPGHLVDDL
                     AAAKIAADRGSPAIDQLAFADFLSHGEFDRHLRRMRPMYRRRRDALLKALRERVPDLG
                     PAGISAGLHLVTWLPSDLDETTVVNAATRRGLGVYGVTPYYICAKGDGGLIFGYATLN
                     ERTITEGINILAAVINEVRSA"
     gene            1800..2102
                     /locus_tag="VEO00_03495"
     CDS             1800..2102
                     /locus_tag="VEO00_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002050969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HYU57099.1"
                     /translation="MTTKALLVRLEARPGKEEEVAQFLTGAVPAVQEEPATTAWFALR
                     LGPSSFGIFDAFPDEDGRQAHLSGRVAEALMARADELLTAAPIIDTVDVLADKLPG"
BASE COUNT          327 a          789 c          728 g          315 t
ORIGIN      
        1 ggtcgccggt ccaccatccc gttccagacc ctgcgcgcct cgtcgtatcc cgagtcgccc
       61 ggcaggagca gctgtccgtc cagccggttc tcgatgtggt ttgccgctga ggttgtcata
      121 cctgcgagcg tgacaggaat cggtactccc tataaggtcc gatctgatgg ccggatcgag
      181 gaccaattct catctcgcga atgaccttct cgtcgcgctc cgccgtgacg ctcaggtccc
      241 gctgcaccgg cagatcgagg catcgatccg cgacggaatc cggtcgggac gactgcggct
      301 cggcacctcg ctgccgccca cccgaaccat cgccgccgac ctcggggtct cgcgcggggt
      361 cgtcgtcgag gcataccagc agctcctcgc cgaggggtac ctgaccagcc ggatgggcgg
      421 ctacacccag gtcgcgatag gcccggagcc cgccccgacg ccgccgcggc cggttcccgc
      481 acgggcggcg aggatcgact tcggctacgg ccgggccgac gtgtcccagt tcccccgggc
      541 cgcgtggcta cgctcgctcc gcagggtgct tgccgaagcc ccgagccggc ggttcggcta
      601 ccccagcggc cgcggtgtcc cggaactgca cgaggcactc gccgactacc tgaaccgggt
      661 gcgaggcacg tcagccaagc cggacaacgt cgtgatctgc aacggctacg cgcaagggat
      721 cacgctgctc atccaggtgc tcgcgaagtc gggggcgaaa cggctcgccg tcgaagaccc
      781 gtcctccgac gacgacgccc gtccgatcgc cgtgtcggcc gggctcgacg tggttggtat
      841 cccggtcggc cacgacggca tccgcgtcga tgtgctcgac cgtaccgacg ccgatgccgt
      901 cgtcctgacc ccgtcacatc agtggccgac cggcgccgtg ctgccggccg cgtcccgcgc
      961 cgcggtgctc gcctgggcga agcggcgcgg ggcgctgatc gtcgaggacg actacgacgc
     1021 ggagtaccgc tacgaccggg ccccgatcgg ggccatgcag ggcctggcgc cggaccacgt
     1081 ggtctacgca ggctcggcga gcaagaccct tgcgcccggg ctccgactcg gctggctcgt
     1141 ggcgcctggc cacctggtcg acgacctcgc cgcggccaag atcgccgcgg accgcggctc
     1201 accggccatc gaccagctcg cgttcgccga cttcctctcc cacggcgagt tcgaccggca
     1261 cctgcgacgg atgcggccga tgtaccgacg gcgccgggac gcgctcctga aggccctgcg
     1321 ggagcgcgtg cccgaccttg ggccggccgg catctccgcc ggcctgcacc tcgtcacctg
     1381 gctgccgtcc gacctcgacg agaccacggt ggtgaacgct gcgacccgac ggggactggg
     1441 tgtctacggc gtcacgccgt actacatctg cgccaaaggc gacggcggcc tgatcttcgg
     1501 ctacgccacc ctcaacgagc gcaccatcac cgaggggatc aacatcctcg ccgcggtgat
     1561 caacgaggtg cgttccgcgt gatccgaatc agcccgcgcc atgaacccgc ccctcgatga
     1621 tcccgcgccg ggagcgcgtc aacgtcccgt gatcgcctat caccaacccc tttggaatcg
     1681 atcatttaga gatacggtgc actcgctcgc tccacgcggc ggccgcgacg tccgggcgct
     1741 gcgtggggtc ggcgcgggac gagcagggcg gctgcgtatc ggaaacgggg agggcgacca
     1801 tgacgacgaa ggcgctgttg gtccggctgg aggccaggcc gggcaaggag gaggaggtcg
     1861 cgcagttcct gacaggcgcc gtgccggcgg tgcaggagga gccggcgacc accgcctggt
     1921 tcgctctgcg gctgggcccg tccagcttcg ggatcttcga cgcattcccc gacgaagacg
     1981 gccgacaggc tcacctttca ggccgggtcg ccgaagcgct gatggctcgg gcggacgaac
     2041 tgctcacggc agcgccgatc atcgacacgg tcgacgtcct cgccgacaaa ctgcccggct
     2101 gagaactgcg cacgcccgtg gccaagcacg cgggcgccag ataaagccgg ccgaccgtg
//
LOCUS       DASPWO010000152         4702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_615541, whole genome shotgun sequence.
ACCESSION   DASPWO010000152 DASPWO010000000
VERSION     DASPWO010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615541"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="VEO00_03500"
     CDS             <1..1383
                     /locus_tag="VEO00_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYU57100.1"
                     /translation="CGVCVDVCPAKSKTEVRHKAINMEPASEHRDAERPNWDFFLSIP
                     ELDRDLLAHDSIKGSQPLEPLFEFSGACSGCGETPYIKLVTQLFGDRIVVANATGCSS
                     IYGGNLPTTPWTANREGRGPAWNNSLFEDNAEFGLGMRLGLEVQIELARRLLADLAPV
                     VGEELARSILDAEQETEPAIHAQRERVAELKGALLDADLPDDPAIRHLMAVADTLVRK
                     GVWIIGGDGWAYDIGFGGLDHVLASGRNVNILVLDTEVYSNTGGQASKATPRGAVAKF
                     AASGKGTGKKDLGAIARAYGNVYVAQISMGANDMQTVKALIEADAWPGPSLVIAYSTC
                     IAHGIDMSKSMSHQKDAVRSGYWPLYRFQPSEIGDGHPFKLDSKTPSIPVRDFVSTEA
                     RFAVLSRTHPDRAEHLLALAQADVDERWRYYEQLSAMERTVPHEPEPDIQPEVESDAG
                     YRYDGEEASR"
     gene            1380..2462
                     /locus_tag="VEO00_03505"
     CDS             1380..2462
                     /locus_tag="VEO00_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HYU57101.1"
                     /translation="MSPTGGAPDRRATNAGGPDLTTTYVGLLLRSPLVASASPITRDA
                     EKVVRLAEAGAGAVVMPSLFEEEILHDEIELSRALEAGAEHFPEALDYFPAVPDYDTT
                     ADRYLSTLRDLKAAVRIPVIASLNAASSGAWIRYARLLEEAGADALELNLYRVAADPD
                     RTAAEMEGSDLRLIADVRAAVGIPLAVKLSPHYTAFANFARRVVEAGAEGLVLFNRFF
                     QPDLDLETLDVVPRIELSHPWEMRLPLRWIAILRPQLVGTASLAATTGVHSGLDAAKL
                     LLVGADVVMTTSALLHSGPERIGAMEAELLAWMTEREYESVAQLRGSVSRSAAEDPAA
                     FERANYVRTLYSWTAPTELTPSSPTG"
     gene            2931..>4702
                     /locus_tag="VEO00_03510"
     CDS             2931..>4702
                     /locus_tag="VEO00_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYU57102.1"
                     /translation="MVHERGRGVSKALLGTLSLVAVVLAGPGAPAGAAPSTSGQAESD
                     NATVYVVQMADDPIVAYEGGVRGFEPTKPAPGEHVDPDSARVRRYEDYLNRSHAAAIA
                     AAGAPADAKFYDYAYSYNGFAAVLTRSQAAQLASQRKVVAVSVDDHREPLTDNTPSDL
                     GLTGEGGFWGSGGPGGTDAVGEDVIIGVIDTGIWPEHPSFSDQSDFTYRPGNAGRALR
                     VYGPPPAQWHGTCQGGELFSAKSCNNKLIGARYFVSGLTLAGASLEGDYLSARDHDGH
                     GTHTASTAGGNRGVDPSIFGRDLGVDTISGMAPRARIAAYKGCFGEAGCVVSDLVAAI
                     DAAVHDGVDVINYSIGSNTPSVLSADAIAFLFASDAGVFVATSAGNAGPEPGTVGAPA
                     AAPWVTTVGASTHSRVFANTVTLGNGATYIGGSVTHGIGSTRLVDGGEECPDGLDPAT
                     VSGAIVLCLRVTGIPRVLHGAGVAAAGGVGMILYDPPQVNVTPTDNHVVPTSTVSGPD
                     GAAIADYIAGAGDAATASLTAGSAAPNTGAPDMAVFSSRGPNGAAPDIIKPDVTAPGV
                     QILAGMTPEPAFSADVPEVPGEYY"
BASE COUNT          750 a         1678 c         1595 g          679 t
ORIGIN      
        1 tgcggggtgt gcgtggacgt gtgcccggcc aagagcaaga ccgaggtgcg gcacaaggcc
       61 atcaacatgg agccggcctc cgaacaccgc gacgcggagc gccccaactg ggacttcttc
      121 ctgtcgatcc ccgagctcga ccgggacctc ctggcgcacg actccatcaa gggatcgcag
      181 cccctggagc ccctgttcga gttctccggg gcgtgctcgg gctgcgggga gaccccatac
      241 atcaagctcg tcactcagct cttcggcgat cgcatcgtcg tggccaacgc cacggggtgc
      301 tcctcgatct acggcggcaa cctccccacc acaccctgga cggcgaatcg ggaaggacgg
      361 gggccggcgt ggaacaactc gctgttcgag gacaacgccg agttcgggct tgggatgcgc
      421 ctcggcctgg aagtgcagat cgagctggca cggcggctgc tggccgacct ggccccggtg
      481 gtcggggagg aactggcccg atcgatcctt gacgccgagc aggagaccga gcccgccatt
      541 cacgcgcagc gcgagcgggt cgccgagctc aagggggcgc tcctggacgc cgacctgccc
      601 gacgatcccg cgattcggca cctcatggcc gtggccgaca ccctggtccg caagggcgtc
      661 tggatcatcg gcggcgacgg gtgggcctac gacatcggct tcggagggct cgaccacgtg
      721 ctggcgtcgg gaaggaacgt gaacatcctg gtgctcgaca cggaggtgta ctcgaacacc
      781 ggagggcagg cctccaaggc cacgccgcgg ggcgcggtgg cgaagttcgc cgcctcgggc
      841 aaggggaccg ggaagaagga cctcggggcc atcgctcggg cctacggcaa cgtgtacgtg
      901 gcccagatct ccatgggcgc caacgacatg cagacggtga aggcgttgat cgaagccgac
      961 gcctggcccg gtccttcgct ggtcatcgcc tacagcacgt gcatcgctca cggcatcgac
     1021 atgtcgaagt ccatgtcgca tcagaaggac gccgtgcgca gcgggtactg gccgctgtac
     1081 cggttccagc cgagcgagat cggggacggt caccccttca agctggactc gaagacgccg
     1141 tccatcccgg tccgcgactt cgtgtccacc gaggcgcggt tcgccgtcct cagccggacc
     1201 caccccgacc gggccgagca cctgcttgcc ctggcccagg cggacgtcga cgagcgatgg
     1261 cgctactacg agcaactctc ggccatggag cggaccgtcc cccacgaacc cgaacccgac
     1321 atccagcccg aggtggagag cgacgccggc taccgctacg acggtgagga ggcgtcccga
     1381 tgagtccgac cggcggggca ccggatcggc gcgccacgaa cgccggaggc cccgatctga
     1441 ccacgaccta cgtggggctt cttctccggt cgccgttggt ggcctcggcg agcccgatca
     1501 cccgagacgc ggagaaggtc gtccggctgg ccgaggccgg cgccggagcg gtggtgatgc
     1561 cctcgctgtt cgaggaggag atcctccatg acgagatcga actgagccgg gccttggagg
     1621 ccggcgccga gcacttcccc gaggcgctcg actacttccc ggcggtgccg gactacgaca
     1681 ccacggccga tcgctacctg tccacgctgc gggacctgaa ggccgccgtg cgcatcccgg
     1741 tgatcgccag cctgaacgcc gcctcctccg gcgcgtggat ccgttacgcg aggctgctcg
     1801 aggaggcggg agccgacgcg ctcgagctga acctgtatcg ggtggcggcc gaccccgacc
     1861 gcaccgccgc cgagatggag ggctccgacc tccggctgat cgccgacgtc cgggcggcgg
     1921 tcggcatccc gctggcggtg aagctcagcc cgcactacac ggccttcgcc aatttcgccc
     1981 gccgggtcgt ggaggcgggg gccgagggcc tggtgctgtt caaccggttc ttccagcccg
     2041 acctggacct ggagaccctc gatgtggtgc cgcggatcga gctcagtcac ccatgggaga
     2101 tgcggctgcc gctccgctgg atcgcgatcc tgcgcccgca gcttgtcggg accgcctcgc
     2161 tcgccgcgac gaccggagtc cactcggggc tggacgcggc gaagctcctg ctggtcgggg
     2221 ccgacgtggt catgacgacc tcggcgctgc tccactccgg ccccgaacgc atcggcgcga
     2281 tggaggcgga gctgctggcg tggatgaccg agcgcgagta cgagtccgtc gcgcagctcc
     2341 gcggaagcgt cagcaggtcg gcagcggagg atccggcggc gttcgaacgg gccaactacg
     2401 tgcggacgct gtactcgtgg acggcgccaa ccgaactgac gccgtcctcc cccacgggct
     2461 gatcgctccc ccgtcgtcac gacgagcgcc aagcgacccg ccgaaccggc cacggcgggt
     2521 tcgccaatcg gaggcagcca tgtcgcacgg caccctggca gggcccgacg gagcgaagaa
     2581 gatcccgcag acggcccgcc gagtccccgg agcccccgtc accccgtgca ggccggctcc
     2641 ggaacgccaa acgcccgatt ccggaacgga caaacgtcct ttcacggcag ggcaacgggg
     2701 agcggctcga cgagcgcttc acaggtccca tcgctgacga cccccgtcca cgagcgcgct
     2761 tgcacccgcc cattggcatt tcgttacgag gttgacgtgg tcgtcgtcat acaccacgtc
     2821 cgggggcgcg accggttcga gttgcgagcg gtcgaacgag tctgcgaaca tcccgccagt
     2881 aatcgatcca tacgcaagcg cgggctcggc tgggaacacg aggaggcacg gtggttcacg
     2941 aacgcggtcg cggcgtcagc aaggcattgc tcggcacgct cagtttggtt gctgtggtcc
     3001 tggcgggacc tggagcccct gctggcgcgg caccgtcgac gagcggtcaa gcggagtccg
     3061 acaacgccac cgtctacgtc gtgcagatgg ccgacgatcc gatcgtggcc tacgagggcg
     3121 gcgtgcgcgg ctttgagcca acgaagccgg cgcccggcga gcacgtcgac ccggacagtg
     3181 ctcgcgtccg acgctacgag gactatctca accgaagtca tgctgccgcg atcgccgccg
     3241 ccggggcacc cgccgacgcc aagttctacg actacgccta ctcgtacaat ggcttcgcgg
     3301 ccgttctgac ccggtcgcag gcggcccaac tcgcgtcgca gcgcaaggtc gtagcagtga
     3361 gtgtcgacga tcaccgggag cccctgaccg acaacacccc gagcgatctt ggtctgacgg
     3421 gagagggcgg cttctgggga tcgggcgggc ccggggggac tgacgccgtg ggcgaggacg
     3481 tcatcatcgg cgtcatcgac accggcatct ggcccgagca cccgagcttc tccgaccagt
     3541 ccgacttcac ctaccggccg ggcaacgccg gccgtgctct tcgtgtgtac ggcccgccgc
     3601 ccgcgcaatg gcacggcacg tgccaaggcg gtgagttgtt ctcggcgaag agctgcaaca
     3661 acaagctgat cggcgctcgt tacttcgtct ccgggctgac gctcgccggc gcctctctcg
     3721 agggcgacta cttgtcggcc cgcgaccacg acggccacgg cacgcacacc gcgtcgaccg
     3781 ccggcggcaa ccgcggcgtc gacccgagca tcttcgggcg cgacctcggc gtggacacga
     3841 tctcgggcat ggcgccccgc gcccgcatcg ctgcctacaa gggatgcttc ggcgaggcgg
     3901 gctgcgtcgt cagcgaccta gtcgccgcca tcgacgccgc cgtgcacgac ggtgtcgacg
     3961 ttatcaacta ctcgatcggc tccaacaccc cctccgtgct ctcggctgat gcgatcgcct
     4021 tcttgttcgc atccgacgca ggcgtgttcg tcgccacgtc tgcagggaac gcgggccccg
     4081 aaccgggcac ggtcggcgcg ccggcggcgg cgccctgggt gacgaccgtt ggggcgagca
     4141 ctcacagtcg tgtgttcgcc aacaccgtga cgctcgggaa cggtgccacg tacataggtg
     4201 gcagcgtgac gcacggcatc gggtcgaccc gcctcgtcga cggcggcgag gagtgccccg
     4261 acgggctcga ccccgccacc gtgagcggcg ccatcgtcct gtgcctgcgg gtcaccggca
     4321 tccctcgtgt cctgcacggc gctggtgtcg ccgccgctgg cggcgtcggc atgatcctgt
     4381 acgacccgcc ccaggtcaac gtcacgccaa ccgacaacca cgtcgttccg acatcgaccg
     4441 tctcgggccc cgacggcgcc gccatcgccg actacatcgc cggcgccggc gatgcggcga
     4501 ccgcgagtct gacggccggc tccgccgcgc cgaacaccgg tgctcccgac atggccgtgt
     4561 tctcctcgcg gggcccgaac ggggctgcgc cggacatcat caagcccgac gtcaccgcgc
     4621 ccggcgtcca gatcctggcc ggcatgacgc ccgaaccggc gttcagcgcc gatgtcccgg
     4681 aggtccctgg tgagtactac ca
//
LOCUS       DASPWO010000153         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_702124, whole genome shotgun sequence.
ACCESSION   DASPWO010000153 DASPWO010000000
VERSION     DASPWO010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702124"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1141
                     /gene="hpnA"
                     /locus_tag="VEO00_03515"
     CDS             134..1141
                     /gene="hpnA"
                     /locus_tag="VEO00_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HYU57103.1"
                     /translation="MGASSTPVLVTGGTGFVGAHVVRALLRRGERPRCLVRATSTLRN
                     LEGLPVEIVLGDLKDPPSLARALKGVATVYHCAADYRLYAGDPRAIYANNVEGTRNVL
                     RAAAEAGVTKVVYTSSVGVLGLSRDGRPADEETPSALEDMVGHYKRSKYQAERVVAEW
                     VGQGLPVVVVNPSTPVGELDVKPTPTGEMIVRFLRRRMPAYVETGLNVVDVRDVAEGH
                     VLAAEKGRVGERYILGDRNMTLREILQTLADLTGLPAPRLKLPHWIPLAVAAVDTGIA
                     RVTGRSPALPLEGVAMSRHRMFFDARKAVRDLGLPQTPVEEALARAVAWFRENGYAPA
                     F"
     gene            1125..1955
                     /locus_tag="VEO00_03520"
     CDS             1125..1955
                     /locus_tag="VEO00_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57104.1"
                     /translation="MPLRSSARRAEETEGWSTVSAASPSQPLTGIVTALAEELEPLVA
                     RASDLAWERAAGSPVLMGRLAERRVAMIHTGVGGLRAEQGARALLAAFPVTRLVTAGV
                     AGGLSPGLGVGEIVVGREVRRGGAAVGAPDEAWVGRALGREPVPRRAVVVTVDRLVWN
                     RRSKADLWRSLSADGAVVVDMESAAWARVAGERRIPYVVVRSVFDRAEDELPEFLERC
                     WDPERGLSRGRVLRHALLRPRAIPRVLAMGARLRSCAAGLPHLVEKLVAADPGTRAEG
                     "
     gene            1952..>2368
                     /locus_tag="VEO00_03525"
     CDS             1952..>2368
                     /locus_tag="VEO00_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HYU57105.1"
                     /translation="MMRVLDRIDDPRELKELTYDELTQLAGECRGAIIETITKRGGHL
                     ASNLGVVELTLALHRAFDSPRDRLVWDTSNQCYTHKLVTGRRDRFPTIRTPGGLSGFA
                     EPAESPHDALAAGHAGTGLSYALGMSVSLQRDPEEPY"
BASE COUNT          332 a          807 c          880 g          349 t
ORIGIN      
        1 agaactgcgc gatgcactcc ggcttcgagg cctcggtggt tcgtgagctc cccaggaacc
       61 ccggcgacat ggtccgactt gccgcatgga acctcctcgg atgacgcgga ccgcgacgcg
      121 cgcgcggtag gccatgggcg cgtcatcgac tcccgtgttg gtcaccggag gcaccggctt
      181 cgtgggggcc catgtggtta gagccctcct gcgacgcggg gagaggcccc gctgcctcgt
      241 gcgcgccacc agcaccctcc gcaacctcga gggccttccg gtcgagatcg tattgggcga
      301 cctgaaggac ccgccctcgc tcgcacgggc cctgaagggt gtcgccaccg tctatcactg
      361 cgccgcggac taccgcctct acgccggcga cccgcgggcg atctacgcca acaacgtcga
      421 ggggacgcgg aacgtgctgc gcgccgcggc ggaagccggc gtgacgaaag tggtctacac
      481 gagctcggtc ggggtcctcg gcctttcccg cgacggccgg cccgcggacg aggagacacc
      541 ctcagccctc gaggacatgg tcggtcacta caagcggagc aagtatcagg ccgagagagt
      601 cgtcgcggag tgggtgggcc agggattgcc ggtcgtggtc gtgaacccgt cgaccccggt
      661 cggcgagctc gacgtgaaac cgacacccac cggggaaatg atcgttcggt tcctgcggcg
      721 ccgcatgccc gcttacgtgg agacaggcct gaacgtcgta gacgtccggg acgtcgcgga
      781 gggtcacgtg ctggccgccg agaagggccg ggtgggagag cgctacatcc tcggcgaccg
      841 gaacatgacc ctgagggaga tccttcagac gctggcggac ctgaccggcc tcccggcgcc
      901 gcgcctgaag ctgccgcact ggatcccgct tgccgtcgct gccgtcgaca cgggcatcgc
      961 gcgggtcacg gggcggtcgc cggcgcttcc cctcgaaggc gtggcgatgt cgcgtcaccg
     1021 gatgttcttc gacgcccgca aggccgtacg cgacctgggt cttccccaga cgccggtcga
     1081 agaggcgctc gcgagggcgg tcgcctggtt ccgggagaac gggtatgccc ctgcgttcta
     1141 gcgcgcgccg ggccgaggag acggaagggt ggagcaccgt ctccgccgcc tcgccatcgc
     1201 agccgctcac cggcatcgtg accgctcttg cggaggagct ggagccgctg gtcgcgcgcg
     1261 catcggatct cgcctgggaa cgggccgccg gctcgcctgt actaatgggg cgcctggccg
     1321 aacggcgcgt ggcgatgatc catacgggcg tcggggggtt gcgggcggag cagggcgccc
     1381 gggcgctgct cgccgcgttt cccgtcacac gcctcgtcac cgccggtgtg gcgggcggtc
     1441 tttccccggg gctcggcgtg ggcgagatcg tcgtggggcg cgaggtgaga cggggggggg
     1501 ccgcggtcgg cgcgcccgac gaagcgtggg tcgggcgggc gctcggccgc gagcccgtcc
     1561 cccgtcgcgc cgtcgtcgtc acggtcgatc ggctggtgtg gaaccgccgg agcaaggcgg
     1621 atctgtggcg ctcgctctcg gccgacggcg cggtcgtcgt ggacatggaa tcggcggcgt
     1681 gggcgcgggt cgccggtgag cgacgaatcc cgtatgtcgt cgtgcggagc gtgttcgaca
     1741 gagccgagga cgagttgccc gagttcctgg agcggtgctg ggatccggaa cgagggctga
     1801 gccgtggccg ggtcctccgc cacgctctgt tgcgcccgcg agcgatccca cgcgtcctcg
     1861 ccatgggggc acggctgcgc tcttgcgcgg ccggcctccc ccacctggtc gagaagctgg
     1921 tggcggcgga tcccggaact cgcgcggagg gatgatgaga gtgctcgacc ggatcgacga
     1981 ccctcgagag ctgaaggagc tcacctacga cgagctgacc cagctcgccg gagagtgccg
     2041 cggcgcgatc atcgagacca tcacgaagcg gggcgggcat ctcgcttcga acctcggtgt
     2101 cgtcgagctg acgctcgcgc tccaccgggc tttcgacagc ccgcgggacc ggctcgtctg
     2161 ggacacgtcg aaccagtgct acacgcacaa gctggtcacc ggccggcgag atcggttccc
     2221 gaccatccgg acgccgggcg ggctctccgg gttcgcggag ccggcggaga gcccccacga
     2281 cgcgttggcg gccggccacg ccggcaccgg actctcctac gccctgggga tgtcggtgtc
     2341 cctccagcgg gatcccgagg agccgtac
//
LOCUS       DASPWO010000154         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_702152, whole genome shotgun sequence.
ACCESSION   DASPWO010000154 DASPWO010000000
VERSION     DASPWO010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702152"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="VEO00_03530"
     CDS             complement(<1..1540)
                     /locus_tag="VEO00_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HYU57106.1"
                     /translation="MYTELHAASAFSFLRASSLPEDLVARAAELGYPAVALVDRDGVS
                     GAPRFFKAARAAGIRPLVGAELTLEGGGCLPLLVESRAGYQSLCRLITRMKAGRPKGE
                     GRLSLDWPEGSVEGLVALPGVETLGRPPDSDRLARILQAFGAKNVFLDVQRHRRREQE
                     AANQALLDLADALGIRAVATNGVRHAAARGRPLLDVLTCIREKKTLATAGRRLSENAE
                     RHLKAPKAMEALFADRPDLLGNAFALAERLQFTLADLGYRFPDYPVPAGETLHSYLCA
                     MTEAGARERYRPHHEKARRQIERELLLIGKLELSGYFLIVWDIVNFCRREGILVQGRG
                     SAANSAVCYSLGITAVDPVGMELLFERFLSEERGEWPDIDLDLPSGDRRERVIQHVYE
                     RYGAQGAAMTANVITYRDRSASREIGKVLGIPVEEIDRLARTMRRFEYVDPDDTLEQR
                     LARAGLDRGDRRITLFARLFQEIQDLPRHLGQHSGGMVIAQGRLDQVVPLEPATMPGR
                     VVIQW"
     gene            1846..2295
                     /locus_tag="VEO00_03535"
     CDS             1846..2295
                     /locus_tag="VEO00_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57107.1"
                     /translation="MTSRTSTFQGTPTRLATRLAMVCVLFSPVAAGADVVLNWNATAV
                     STMLGQSPAPNPFAQARFMAITQLAVFEAVNAVTGEYQPYLGTIDAATGASADAAAIA
                     AAHAVLLNYFPGSAAALNAERSSSLAAIPDGPAKDDGVAAVRRRRRR"
     gene            2531..3151
                     /locus_tag="VEO00_03540"
     CDS             2531..3151
                     /locus_tag="VEO00_03540"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HYU57108.1"
                     /translation="MTVGGVDSTERPPDRADVARFYAGSSPSFLLNLAAREVSAAQDR
                     SLSHNARALALINMAISDSLVASFFTKYHYNLWRPETAIRLGDTDGNPKTDPDPAFAP
                     FILTPCFPSYPSNHASGSSGGAEMLRRLYGAGGHDITLANPAVPGMTLHYSAFKQITD
                     DVDDARVYSGIHFRFDQEAGGRLGRGVATYIYKHGLRPVHPDGDEE"
     gene            3410..>4019
                     /locus_tag="VEO00_03545"
     CDS             3410..>4019
                     /locus_tag="VEO00_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57109.1"
                     /translation="MDLTTGKIVERVPGMVKAVGCDEIGGVVVGYVTAVRLPGKTATP
                     VPPLSGDRVLARAPGGAWISEGRRISGGRWRGPASVFVLEKGSSRPMDLLPARFGAVG
                     AARPLPTPDSFAVRFGNLLRDGRLVLAAGWQPSRSGRVVEDVPWAFFALDLGTGEAAP
                     LTLPLDSTAAMNQNLLQRIAATPDAAHLVLAVHDGEQMSVAQF"
BASE COUNT          577 a         1423 c         1402 g          617 t
ORIGIN      
        1 cccactggat gaccacccgg cccggcatgg tggcaggctc gagggggacc acctggtcga
       61 gccgcccctg ggcgatgacc atcccccccg agtgctggcc aaggtggcgc ggcaggtcct
      121 ggatctcctg gaagagccgg gcgaagagcg tgatccgccg gtcgccccgg tcgaggccgg
      181 cccgcgccag gcgttgctcg agggtgtcgt cggggtccac gtactcgaac cggcgcatgg
      241 tgcgggccag acggtcgatc tcctctaccg ggatgccgag caccttgcct atctcccggg
      301 aggcgctgcg gtcgcggtag gtgatgacgt tcgcggtcat ggcggcgcct tgggccccgt
      361 agcgttcgta gacgtgctgg atcacccgct cgcggcggtc gccgctcggc aggtcgaggt
      421 cgatgtccgg ccactccccg cgctcctcgg agaggaagcg ctcgaagagc agctccatcc
      481 ccacggggtc caccgcggtg atgcccaggc tgtagcagac cgcgctgttg gcggcggagc
      541 cgcggccctg gaccaggatc ccctcccggc ggcagaagtt cacgatgtcc cagacgatca
      601 ggaagtagcc cgagagctcg agcttgccga tgaggagcag ctcgcgctcg atctgccgcc
      661 gcgccttctc gtggtggggc cggtagcgct cgcgggcccc ggcctccgtc atcgcgcaga
      721 ggtaggagtg cagggtctcc cccgccggca ccgggtagtc gggaaagcga taccccaggt
      781 ccgcgagggt gaactggagg cgctccgcca gggcaaaggc gttccccagg aggtccgggc
      841 ggtccgcgaa gagcgcctcc atggccttcg gcgctttcag atgccgctcg gcgttctcgc
      901 tcagccgccg tcccgccgtg gccagggtct ttttctcgcg gatgcaggtc agcacgtcga
      961 ggaggggccg gcctctcgcg gcggcgtggc gaacgccgtt cgtggccacg gctcggatgc
     1021 ccagggcgtc ggccaggtcg agcagggcct ggttcgccgc ttcctgctcg cgccggcggt
     1081 gccgctgcac gtccaggaag acgttcttcg ctccgaacgc ttggaggatg cgggccaggc
     1141 ggtccgagtc gggtggtcgg cccagcgtct cgaccccggg cagggcgacg agcccttcga
     1201 cgctgccctc cggccagtcg agggacagcc ggccctctcc tttcggacgg ccggccttca
     1261 tgcgcgtgat gagccggcag aggctctggt aacccgcccg gctctccacg agcagaggca
     1321 ggcagcctcc gccttcgagg gtcagctcgg cgcccacgag gggccggatt cccgccgccc
     1381 gcgcggcctt gaagaagcgc ggggcccccg agacgccgtc ccggtccacg agcgccaccg
     1441 ccggataccc gagctccgcc gcccgggcca cgagatcctc gggcagcgac gaggcccgga
     1501 ggaacgagaa ggccgacgcg gcatggagct cggtgtacat ggaggcgagg gccggggata
     1561 gtgctttcgc tttttattcg ccatcgtgcc gtgaaatccc cgtcgcgtca agtcggccgg
     1621 ggggccagtt gagcacagca tcttcgatct tgaccctcgc cccgcaaagg gggtagggtc
     1681 tccgtcaacg tagccaggga ggcgtctagg gtcgaaagct cctctctccc cttgacggcg
     1741 cctccgtagc cgtcgccgcg gatgagaacg ccgatgcttg ccttctccgc accggctaca
     1801 cgcattgctc acgccgggcg tgggctgact gaagggagaa acgccatgac ctcgaggacc
     1861 tcgaccttcc aggggacgcc gacgagactc gccacccgcc tcgcgatggt ctgcgtgctg
     1921 ttcagtccgg ttgccgccgg ggcggacgtc gtcctgaact ggaacgcgac cgcggtctcc
     1981 acgatgctcg gccagagccc tgccccgaac cccttcgccc aggcacgctt catggcgatc
     2041 acgcagctcg cggtgttcga ggcggtcaac gcggtcaccg gcgagtatca gccgtacctc
     2101 ggaacgatcg acgcggccac cggcgcatcc gccgacgccg ccgcgatcgc ggcggcacac
     2161 gcggtgttgc tgaactactt ccccggcagt gcggcggccc tcaacgccga gcgttcgagc
     2221 tccctggcgg cgataccgga cggcccggcc aaggacgacg gcgtcgccgc cgtgaggcgg
     2281 cggcggcggc gctgatcctg cttcgcgcca acgacggctc ggcgcccgcc gagttcttcg
     2341 tgcccggctc gccggggccg ggggtctggg cggccacgcc gagctgcccg accgtcgggg
     2401 gagtccaggt cggaatcctc ctgcattggc gcaatgtgac gcccttcggc atccgcagcg
     2461 ccgccgactt tctggcggct ccgcgccgtc gctcaccagc gggacctgtc ggaaggacta
     2521 cgtcgaggtc atgacggtcg gcggcgtcga cagcacggag cggccgccgg atcgagccga
     2581 cgtagcgcgg ttctacgccg ggtcctcgcc gagcttcctg ttgaacctcg ccgcccgaga
     2641 ggtgtccgcc gcccaggacc gttcgctctc ccacaacgcg cgggccctgg ccctgatcaa
     2701 catggccatc agcgacagcc tcgtcgcgtc cttcttcacc aaataccatt acaacctgtg
     2761 gcgacccgag acggcgattc gcctcggtga caccgacggc aacccgaaga ccgaccccga
     2821 ccccgccttc gcgccgttca tcctcacgcc ctgttttccc agctatccct caaatcacgc
     2881 gagcggaagc tccggggggg ccgagatgct caggcgcctc tacggcgccg gcggccacga
     2941 catcaccctg gcgaaccccg ccgtcccggg catgaccctg cactacagcg cgttcaagca
     3001 gatcaccgac gatgtcgacg acgcgcgcgt gtacagtggg atccacttcc gattcgacca
     3061 ggaggcaggt ggccggcttg ggcggggcgt cgcgacctac atctacaagc acggcctgcg
     3121 cccggtgcac cctgacggtg atgaagagtg aaccccgtca gaagccgggc gtgaagttga
     3181 gctcccagaa ccagctgccg gtgcgctcag acatcccccc ggagctcctg ggactgatgg
     3241 cggaccactg cgcgtgattc gagtcttcct cgttcttgcc gcctgcccgc tctgggtgat
     3301 gtgcagcgga tcgtcctcgc ggggaggcgc cgcccacggg tattggagcg agagctgtct
     3361 cagcggagac ggacggtatc tgctggcggg aggcgatcac gcggggctgg tggacctcac
     3421 caccgggaag atcgtcgagc gcgtgccggg aatggtgaag gcggtcggct gcgatgagat
     3481 cgggggcgtg gtcgtcggct acgtcacggc ggtccggctg ccggggaaga ccgccacccc
     3541 cgttcccccg ttgagcggcg accgagtcct cgcccgggct cccgggggcg cctggatctc
     3601 cgagggccgg cggatctccg gcggcaggtg gagagggccc gcgtcggtct tcgtcctcga
     3661 gaagggctcg tcgcggccga tggacctgtt gccggcgcgc ttcggcgcgg tgggagcggc
     3721 caggcctctc cccacgcccg actcgttcgc ggtgcgcttc ggaaacctcc tgcgcgacgg
     3781 ccgcctggtg ttggccgcgg gctggcaacc ctcacgaagc gggcgcgtgg tcgaagacgt
     3841 cccctgggca ttcttcgcgc tggacctggg cacgggcgaa gcggcgcccc tgaccctgcc
     3901 cctcgactcc accgcggcga tgaaccagaa ccttctccag aggatcgcgg ccacgccgga
     3961 cgccgctcac ctggtgttgg cggtgcacga cggcgagcag atgtcggtcg cgcagttcg
//
LOCUS       DASPWO010000155         4458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_279895, whole genome shotgun sequence.
ACCESSION   DASPWO010000155 DASPWO010000000
VERSION     DASPWO010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279895"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1344
                     /gene="ahcY"
                     /locus_tag="VEO00_03550"
     CDS             91..1344
                     /gene="ahcY"
                     /locus_tag="VEO00_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HYU57110.1"
                     /translation="MDCDVKDLALAEKGRERIEWADHEMRVLTLIRERFEKERPLAAL
                     RIAACLHVTTETANLMRALVAGGAEVALCASNPLSTQDDVAAALCEDGIDVYAVRGED
                     TETFFRHIRAVLDTHPNVTMDDGCDLVTVLHKERRDQLPELIGGTEETTTGVIRLRAM
                     AAEGVLAYPIVAVNDADTKHLFDNRYGTGQSTIDAIVRSTNLLLAGRTVVVCGYGMCG
                     RGVASRARGLGAKVVVTEVSPLAALEAVMEGFQVMPVREAARVGDVFVTVTGDTHVLR
                     GEHFEVMKDGAVLANSGHFDVEIEKPALAALAASTRRIRDVVDEYRLKDGRRLYLLAE
                     GRLVNLGAAEGHPAAVMDMSFANQALSVEWIAKHRGTLEVRVHGVPDDIDREIAALKL
                     WAMGVEIDRLTDEQEAYLRSWESGT"
     gene            1404..1673
                     /locus_tag="VEO00_03555"
     CDS             1404..1673
                     /locus_tag="VEO00_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57111.1"
                     /translation="MFLLSAIDGNPHQVRIEEVRLRTPGSRAEGHALRTFPMVLEVQP
                     GPELVVPFDVRSTEVPRGTYAVGARVLVDGIAAPEALEQAELRVR"
     gene            1712..2401
                     /locus_tag="VEO00_03560"
     CDS             1712..2401
                     /locus_tag="VEO00_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HYU57112.1"
                     /translation="MLDLVFPPACVACGGPGWPWCPRCEAEVAVLTAPGCRRCGRPLE
                     HAVSRCRDCPPPPIAWLRAPFGFDGPVRTTVHALKFGGQFVLADALSEAMVETAGRDP
                     PGEVVTWVPLSARRRAARGFDQAERLARAVAARLGRHAAGLLERASDTAPQARRGRAE
                     RRRALRGAFRATGPAPRSVLLVDDVLTTGSTAAECARALRRSGARAVSVLVAARALAE
                     PLPARCRLGTG"
     gene            2706..3080
                     /gene="raiA"
                     /locus_tag="VEO00_03565"
     CDS             2706..3080
                     /gene="raiA"
                     /locus_tag="VEO00_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HYU57113.1"
                     /translation="MELVMRGRNGRLPESFKRIAEQKLGRLEHVDPRLTRVEVEWLEE
                     HNPRISDAHERVKAVARTSRLTFRARGAGPDVAAALDQVVARLDRQLTSYRSRFRARL
                     IAGAHRLQSAARRTSGDEMTLP"
     gene            3122..3823
                     /locus_tag="VEO00_03570"
     CDS             3122..3823
                     /locus_tag="VEO00_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU57114.1"
                     /translation="MAGKTPKRPVRPEGERLKVLIVDDHALFRRGLLMTLEAEEDIEV
                     VGEAADGNEAVDKSVELMPDVVLMDVRMPKHSGIEATEQIRDLVPSAKILVLTNSEEE
                     ADLYDAIKAGASGYLLKEISIEEVAEAIRSVQAGQSRISPAMASKLLNEFAAMSKRPP
                     EQPQLPAPRLTDREMEVLTLVAKGMNNREIAGQLFISENTVKNHIRNILEKLHLHSRM
                     EAVVYAVREKLLEIK"
     gene            3827..>4458
                     /locus_tag="VEO00_03575"
     CDS             3827..>4458
                     /locus_tag="VEO00_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU57115.1"
                     /translation="MAAEVGAVPAERVRLVRRNTLLLSAAEPSLRAVLSLTFTVGSIA
                     VFRFSGRESYAGYLTAATFAAAAVAAVVAGRAMDRLGRRPGLAAGHGTLVVAAGVAAV
                     AVSARSAPVLLIAAALFGAGQGAANLGRGAMADMYEPERRGRAVGILLAVGTIGAVGG
                     PILAAGTQAFARNVLGVEPLLFPWAVVAALALLGLTAVLNLRPDPRDLAVA"
BASE COUNT          601 a         1536 c         1697 g          624 t
ORIGIN      
        1 gatccgcatc gccgaacctc cgggcgatcc tggagcgcac gatcccggcc atcgcttccg
       61 ggtgacaccg accagacggg aggagtcgac gtggactgtg acgtgaagga cctggcgctc
      121 gccgagaagg gccgggaacg catcgagtgg gccgaccacg agatgcgggt gctgaccctg
      181 atccgggagc gcttcgagaa ggagcggccg ctcgccgccc tgcgcatcgc ggcctgcctg
      241 cacgtgacca ccgagaccgc gaacctgatg cgggcactcg ttgcgggcgg cgccgaggtg
      301 gcgctctgcg cctcgaaccc gctgtcgact caggacgacg tcgcggcggc gctctgcgag
      361 gacggcatcg acgtctacgc cgtgcgcggc gaggataccg agaccttctt ccgccacatc
      421 cgcgcggtgc tcgacaccca cccgaacgtg acgatggacg acggctgcga cctggtgacg
      481 gtgctccaca aggagcggcg cgaccagctg cccgagctga tcggggggac cgaggagacc
      541 accaccggcg tcatccggct gcgggcgatg gccgccgagg gcgtgctcgc gtacccgatc
      601 gtcgccgtga acgatgccga caccaagcac ctgttcgaca accgctacgg gaccgggcag
      661 tcgacgatcg atgccatcgt ccggtccacg aacctcctgc ttgcggggcg gaccgtggtg
      721 gtctgcggct acggcatgtg cgggcgcggc gtcgcgtcac gcgcgcgcgg gctgggggcg
      781 aaggtggtcg tcaccgaggt cagcccgctg gcggcgctcg aggccgtgat ggagggcttc
      841 caggtgatgc cggtgcgcga ggccgcccgg gtcggcgacg tgttcgtcac cgtgaccggc
      901 gacacccacg tcctgcgggg cgagcacttc gaggtgatga aggacggcgc ggtgctggcg
      961 aactccggcc acttcgacgt cgagatcgag aagccggccc tcgcggcgct ggccgcgtcg
     1021 acccggcgca tccgcgacgt ggtggacgag taccggctga aggacggacg gcgcctctac
     1081 ctgctggccg aggggcggct ggtgaacctc ggggccgccg agggccaccc ggcggcggtg
     1141 atggacatgt cgttcgccaa ccaggccctg tcggtggagt ggatcgcgaa gcaccgcggc
     1201 accctcgagg tccgcgtcca cggtgtgccg gacgacatcg accgcgagat cgcggccctg
     1261 aagctctggg cgatgggggt cgagatcgac cggctgacgg acgagcagga ggcgtacctg
     1321 cgctcctggg aatcggggac ctgatcgggc acggcatccg gatccgcttc aagagcgagc
     1381 gcctggcggt cggcgtccgc ggggtgttcc tgctctccgc catcgacggg aacccgcacc
     1441 aggtccggat cgaggaggtc cggctgcgga cgcccggctc gcgggccgag ggccacgcgc
     1501 tgcgcacgtt cccgatggtg ctcgaggtcc agcccggccc cgagctggtg gtgccgttcg
     1561 acgtccgctc gaccgaggtc cccaggggga cctacgcggt gggcgcgcgg gtactggtgg
     1621 acggaatcgc cgcccccgag gccctggagc aggcggagct cagggtccgc tagccggcgg
     1681 tcgtgtcggt ggcgtgcgct agcgtgcttg cgtgctcgac ctggtgtttc cgccggcgtg
     1741 cgtcgcgtgc ggggggcccg gatggccgtg gtgcccgcgg tgcgaggcgg aggtggccgt
     1801 gctcaccgcg ccggggtgcc ggcgctgcgg cagacccctg gagcacgccg tctcgcggtg
     1861 ccgggactgt ccgccgccgc cgatagcctg gctgcgcgcc ccgttcgggt tcgacggacc
     1921 ggttcgcacg acggtccacg cgctgaagtt cggtgggcag ttcgtcctgg ccgatgcgct
     1981 ctcggaggcg atggtcgaga cggcggggcg cgatccgccg ggcgaggtcg tcacctgggt
     2041 cccgctgtcg gcccgccggc gggcggcgcg cgggttcgac caggccgagc ggcttgcccg
     2101 ggcggtcgcc gcacggctcg gtcggcacgc tgcggggctg ctcgagcggg cgtccgatac
     2161 ggctccgcag gcccgccggg gaagggccga gcgccgccgg gcgctgcgcg gcgccttccg
     2221 cgccaccggc ccggcgccgc gctcggtcct tctggtcgac gacgtcctga cgacggggtc
     2281 gaccgcggcc gagtgcgccc gggccctccg ccggtccggg gcgcgcgccg tgtcggtgct
     2341 cgtggccgcc cgggcgctgg cggagcccct tccggcccgg tgtagactcg ggactggatg
     2401 accgtctggg tctgtggttg ctcgaggatg ttcctcgaaa gccgatgcca gtcgcaggcg
     2461 aagcggccca cgtaaggcga cccttggtcg ctgagcatgg tgcggtttag acgtaagccc
     2521 tgcctcgggg cggctccggc cgtgccggga gaaggcgcta agccgcctct tcggcggcga
     2581 acccctcagc aggcgagtgt gggggcaaag accaggtcag ccggacgtca tccgacggga
     2641 agggccggcg cgctcgcgtc ggcccttcgc gcgtctctcg gatccccagc agagaggggg
     2701 ggagcatgga gttggtgatg cgtgggcgga acggtcggct cccggagtcc ttcaagcgga
     2761 tcgcggagca gaagctcggg cgcctcgagc acgtggatcc gcgactgacg cgggtcgagg
     2821 tggaatggct cgaggagcac aatccgcgca tctccgacgc acacgagcgc gtcaaggcag
     2881 tcgcccgcac ctctcggctc acgttccggg cgcgcggcgc ggggcccgac gtggcggccg
     2941 ccctcgatca ggtggtggcc cgcctggacc gccagctgac cagctaccgc tcacgcttcc
     3001 gtgctcggct gatcgccggg gcccataggc tacaatcggc agcgcgccgc acgagcggcg
     3061 acgagatgac cctcccgtag cgcgagtcga cgggactcca ccgcgggcga ggtggcccgg
     3121 tatggcagga aagacgccga aacgaccggt acgacccgag ggtgagcgcc tgaaggtcct
     3181 gatcgtcgac gaccacgccc tgttccgccg cggcctgctg atgacgctcg aggccgagga
     3241 ggacatcgag gtcgtcgggg aggccgccga cgggaacgag gcggtcgaca agtcggtcga
     3301 gctgatgccc gacgtcgtgc tgatggacgt gcgcatgccg aagcactcgg gcatcgaggc
     3361 caccgagcag atccgcgacc tggtcccgtc ggcgaagatc ctcgtgctga cgaactccga
     3421 ggaggaggcc gacctctacg acgcgatcaa ggccggcgcg tccggctacc tgctgaagga
     3481 gatctcgatc gaggaggtgg ccgaggcgat ccgctccgtg caggccgggc agtcccggat
     3541 cagcccggcg atggcttcca agctcctgaa cgagttcgcc gcgatgagca agcgcccgcc
     3601 cgagcagccc cagctgcccg cgccgcggct caccgaccgc gagatggagg tcctgacgct
     3661 cgtggcgaag gggatgaaca accgcgagat cgccggccag ctcttcatct ccgagaacac
     3721 ggtgaagaac cacatccgca acatcctgga gaagctgcac ctgcactcgc ggatggaggc
     3781 cgtcgtgtac gccgtccgcg agaagctgct cgagatcaag tagaccgtgg cggcggaggt
     3841 tggggccgtc cccgcggaac gggtccggct ggttcgccgc aacaccctct tgctctccgc
     3901 cgccgagccg tcgctgcgcg ccgtcctctc gctgaccttc accgtggggt cgatcgcggt
     3961 gttccgcttc tccggccggg agtcctacgc cgggtacctg accgcggcca cgttcgccgc
     4021 cgccgccgtc gctgccgtcg tggccggccg ggcgatggac cgcctgggcc ggcggccggg
     4081 gctcgccgcc gggcacggga cgctcgtcgt ggcggccggc gtcgcggcgg tggccgtctc
     4141 cgcccgctcg gccccggtgc tgctgattgc cgcggccctg ttcggtgccg ggcagggagc
     4201 ggcgaacctc gggcgcggcg ccatggccga catgtacgag cccgagcgcc gcgggcgcgc
     4261 ggtcgggatc ctgctggccg tcggcaccat cggggccgtc ggggggccga tcctcgccgc
     4321 ggggacgcag gcattcgccc gaaacgtgct cggggtcgag ccgctgctct tcccgtgggc
     4381 ggtcgtggcg gccctggccc tcctcgggtt gacggcggtg ctcaacctgc ggcccgaccc
     4441 gcgcgacctc gcggtcgc
//
LOCUS       DASPWO010000156         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_364685, whole genome shotgun sequence.
ACCESSION   DASPWO010000156 DASPWO010000000
VERSION     DASPWO010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364685"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VEO00_03580"
     CDS             complement(<1..607)
                     /locus_tag="VEO00_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU57116.1"
                     /translation="MTDTRDRPSFLVEGELYEAAPWWIRRAPAALRDVHERESYRWWV
                     LVTVLAGMFATGLTITVLTTAVPVIRRDFHASLASLSWVVSAPFLFRSIFVPVFGKTG
                     DLIGRRRTWLLGFAVSATASLFCGFAPGVGFLILFRALAAVGSAAVIPSALALIAVAF
                     EPDERVKALGWWSATIAVAPLLGVIAGGFVVEAFGWRWIFFG"
     gene            complement(604..>2041)
                     /locus_tag="VEO00_03585"
     CDS             complement(604..>2041)
                     /locus_tag="VEO00_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU57117.1"
                     /translation="VEEVPSVAWPLLLRERLRRRVRESDRYRWWVLWTVLAGLFAAGF
                     TITVLAVSLPDIARDLGADPTSLTWIVTGPFLAHALGMPLLGKVGDVYGHRRVYLLGF
                     GLFTVFTGLTALAWNAPSLITVRIVGALEGAATGPASMALIMHAFPAEERVKAMGWWA
                     LVGAGAPVVGLVAGGPIVDAFGWRWLFVAQVPLALTALVAAVALLHETPRRPRVPIDV
                     RGAAALAGATVFPLLALQIGRDRGWTDAVPVGLLAAGAIALALFVRVERRAREPLLPL
                     DFFRRRNFTASLVAQSFSQFAYMGGFIVSPLLMQNRFGYTVSGTALIMLCRPLSFSLS
                     SPASGYVTVRVGERRAAVTGTTLVVVSMACFAIAAAQESAPLVVVALVLSGLGLGASS
                     PSLITSAANAVEPEHLGVANAAQQMMTLIGAVAGMQVLATVQAGGAGGRFALAYLIGL
                     AVAVPAVVAAGFVRSSARGAELRVARAA"
BASE COUNT          370 a          748 c          732 g          191 t
ORIGIN      
        1 gcccgaagaa gatccagcgc cacccgaatg cctcgaccac gaagccgccg gcgatcacgc
       61 ccaggagcgg cgccaccgcg atggtggccg accaccaccc gagggccttc acccgctcgt
      121 ccggttcgaa cgccaccgcg atcagcgcca gcgcgctggg gatgaccgcg gcgctgccga
      181 ccgcggccag ggcccggaag aggatcagga agccgacgcc gggcgcgaag ccgcagaaga
      241 gcgaggccgt ggccgagact gcgaagccca gcagccaggt gcggcgacgg ccgatgaggt
      301 ctcccgtctt gccgaagacc ggcacgaaga tcgaccggaa gaggaacggc gccgagacga
      361 cccacgacag ggaggcgagc gacgcgtgga agtccctccg gatgacgggg accgcggtgg
      421 tcaggacggt gatggtgagc ccggtggcga acatgcccgc caggaccgtc accagcaccc
      481 accaccggta cgactcgcgc tcgtgcacgt cgcgcagcgc ggcgggagcg cgccgtatcc
      541 accagggcgc cgcctcgtag agctcgccct cgacgaggaa ggacggccgg tcgcgcgtgt
      601 cggtcatgcg gcgcgcgcga ctcggagctc ggcaccccga gcgctcgagc ggacgaagcc
      661 ggcggccacg accgccggta ccgcgaccgc cagcccgatg agataggcga gcgcgaaccg
      721 cccgcccgcc ccgccggcct ggacggtggc caggacctgc atccccgcca ccgcgccgat
      781 gagcgtcatc atctgctggg cggcattcgc aaccccgagg tgctcgggct ccaccgcgtt
      841 ggcggccgag gtgatcagcg acggtgacga cgcgccgagg cccaggccgg agagcacgag
      901 cgccaccacc acgagcggcg ccgactcctg ggccgccgcg atggcgaagc aggccatcga
      961 gaccaccacc agtgtcgtcc cagtcaccgc cgcgcgccgt tcgcccaccc gcaccgtgac
     1021 gtaccccgat gcgggcgacg acaggctgaa cgacagcggg cggcacagca tgatcagcgc
     1081 ggttccggac accgtgtacc cgaaccggtt ctgcatcaac agcgggctca cgatgaaccc
     1141 gcccatatag gcgaactgcg agaagctctg ggcaaccagg gacgcggtga agttgcggcg
     1201 gcggaagaag tcgagcggca gcaacggctc gcgtgcccga cgctcgaccc gcacgaagag
     1261 cgcgagagcg atcgcgcccg ccgcgagcaa cccgacgggc accgcgtcgg tccacccccg
     1321 gtcccgcccg atttggagcg cgaggagggg aaagacggtg gcgccggcga gtgcggccgc
     1381 tccccgtacg tcgatcggca cgcgcggtcg ccggggtgtc tcgtggagca acgcgaccgc
     1441 cgcgacgagc gccgtcaacg ccagcggcac ctgggcgacg aacagccacc gccatccgaa
     1501 cgcgtcgacg atcggtccgc ccgccaccaa cccgacgacg ggggcaccgg cgccgacgag
     1561 cgcccaccaa cccatcgcct tcacgcgctc ctcggccggg aaggcgtgca tgatcagggc
     1621 catcgacgcc ggcccggtgg cggcaccctc gagcgcgccg acgatgcgca cggtgatcag
     1681 cgacggcgcg ttccaggcca aggcggtgag cccggtgaag accgtgaaca gcccgaagcc
     1741 gagcaggtac acgcggcggt ggccgtagac gtcgccgacc ttccccaaca ggggcatgcc
     1801 gagcgcgtgg gcgaggaacg ggccggtgac gatccaggtc aggctggtcg ggtcggcgcc
     1861 gaggtcccgg gcgatgtcgg ggagcgagac cgccagcacc gtgatggtga acccggccgc
     1921 gaagaggccg gcgaggacgg tccacaggac ccaccagcgg taccggtcgc tctcccggac
     1981 ccggcggcgc agtcgctcac gcaggagcag cggccaggcg accgacggga cctcctcgac
     2041 a
//
LOCUS       DASPWO010000157         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_955405, whole genome shotgun sequence.
ACCESSION   DASPWO010000157 DASPWO010000000
VERSION     DASPWO010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955405"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1815
                     /locus_tag="VEO00_03590"
     CDS             100..1815
                     /locus_tag="VEO00_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYU57118.1"
                     /translation="MTARPPARRLIGMLVAFVLGLSAIGARLVLLQVKDAEAFQDLAR
                     QQRVYTIDLPAPRGGIFDRAGNELAVSLPAQAVFADPSFVTDPLGEARRLAPLLRVPA
                     RALEQKLSGDSRFVYLARQVRPRLAGRIRALGLAGIGFLDESRRAYPAGILAPQVLGF
                     VGVDGQGLAGLELQYQDVLAGAPGTRVQEQDPAGHGIPGGERSEVAPVPGESLVLTID
                     KDVQFEAQRALAAAVKRNGAKGGTVLVMEPATGDILAMASYPLFDPAHYETADPQTVR
                     ARAITDVYEPGSVNKVITASAAVEESLFPLDRAFLIPDSYQVAEKVFHDAHAHPEEPM
                     TLADVIAYSSNVGTIKVAQRLGQPRLADYLERFGFGRRTGIAFPGESRGILPPRDGWW
                     GNSMGTIPIGQGIAVTPLQMASVYQTIANGGVWVQPRLVRGVTGADGEFTPAGAARHR
                     RVVTGFTAERVTEMLAYAVEVGTGGAAAVPGYWVAGKTGTARKPLENARGYSDRYVAS
                     FIGFLPASAPRVVIAAILDEPQTVYGGIASAPLFREVARYALAHLRVPSAPRPAVPPH
                     VVPID"
     gene            1823..>2475
                     /locus_tag="VEO00_03595"
     CDS             1823..>2475
                     /locus_tag="VEO00_03595"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HYU57119.1"
                     /translation="MRFVGDSGLETEPGEAFPAAPRSAMQAPPDLPLAELVAPLAEAS
                     VRGDASVRIHDVTYRSAEAEPGSLFFAVPGGRSDGHDFVADAVARGAVAALVEHPVAA
                     KVTQVVVPSVRVAMGPVAAAFFGRPAARLTLVGVTGTNGKTTTTYLLEAVFRAAGMTP
                     GVIGTTGVRVDGGPLPLERTTPEAPDLHRLLYRMAEEGVGAVAMEVSSHGLHQHRVGG
                     "
BASE COUNT          304 a          888 c          953 g          330 t
ORIGIN      
        1 accgtcccga cctccgaacg agggggagcg ggccgatgac cgcccgccac cgggcgcggg
       61 agcgccgatg accgcccacc accgggcgcg ggagcgccga tgacggcgcg gccgccggcc
      121 cgacggctca tcgggatgct cgtggcgttc gtcctgggcc tctcggcgat cggggcccga
      181 ctggtgctgc tgcaggtcaa ggacgccgag gccttccagg acctggcgcg gcagcagcgc
      241 gtctacacca tcgacctccc ggcgccgcgg ggcgggatct tcgaccgggc cggcaacgag
      301 ctggccgtat cgctcccggc ccaggccgtg ttcgccgacc cgtcgttcgt caccgatccc
      361 ctcggcgagg cgcgccggct ggcgccgctg ctgcgggtcc cggcccgcgc gctggagcag
      421 aagctgtccg gggactcccg gttcgtctac ctggcccggc aggtccggcc gaggctcgcc
      481 ggacggatcc gagcgctggg cctggccggg atcgggttcc tggacgagag ccggcgggcc
      541 tatccggcgg ggatcctggc gccccaggtg ctcgggttcg tcggcgtgga cgggcagggc
      601 ctggccgggc tcgagctgca gtaccaggac gtgctggccg gcgcgccggg gacgcgcgtg
      661 caggaacagg accccgccgg ccacggcatc cccggcggcg agcgctcgga ggtcgcgccg
      721 gtgcccgggg agagcctggt gctgacgatc gacaaggacg tgcagttcga ggcgcagcgc
      781 gcgctcgccg cggcggtgaa gcgcaacggc gccaagggtg ggacggtgct ggtgatggag
      841 ccggccaccg gggacatcct ggcgatggcg tcgtacccct tgttcgaccc ggcccactac
      901 gagacggccg acccgcagac ggtccgcgcc cgcgcgatca cggacgtcta cgagccgggc
      961 tcggtgaaca aggtgatcac ggcgtcggcg gcggtcgagg agagcctgtt cccgctcgac
     1021 cgggcgttcc tgatcccgga ctcgtatcag gtggcggaga aggtcttcca cgacgcgcac
     1081 gcccacccgg aggagcccat gacgctcgcc gacgtgatcg cctactcgtc gaacgtcggc
     1141 acgatcaagg tggcacagcg gctggggcag ccgcggctgg ccgactacct ggagcgcttc
     1201 ggcttcgggc ggcgcacggg catcgcgttc ccgggcgaat cgcgcggcat cctcccgccc
     1261 cgcgacggct ggtgggggaa cagcatgggg acgatcccga tcgggcaggg gatcgcggtg
     1321 acgccgctgc agatggcctc cgtctaccag acgatcgcca acggcggcgt gtgggtgcag
     1381 ccgcggctgg tgcgcggggt gaccggcgcg gacggcgagt tcacgccggc cggggccgcg
     1441 cgccatcggc gggttgtcac ggggttcacg gcggaacgcg tgaccgagat gctcgcctac
     1501 gcggtggagg tcgggaccgg cggcgcggcg gccgtccccg gctactgggt cgcggggaag
     1561 accggcaccg cgcgcaagcc gctggagaac gcgcgcggct attcggaccg ctacgtggcc
     1621 tcgttcatcg gcttcctgcc ggcctcggcg ccgcgggtgg tgatcgcggc gatcctcgac
     1681 gagccgcaga ccgtgtacgg gggcatcgcc tccgcgccgc tgttccggga ggtcgcccgc
     1741 tacgcgctgg cccatctgcg cgttccgtcg gcgccgcgcc cggccgtccc cccacacgtc
     1801 gtcccgatcg actgataccc ggatgcgttt cgtgggagac tccgggctcg agaccgaacc
     1861 cggggaggcg tttcccgccg ccccgcgctc cgcgatgcag gctccccccg acctcccact
     1921 ggcggagctc gtggcgccgc tcgccgaagc atcggtgcgc ggcgacgcga gcgtccggat
     1981 ccacgacgtg acctatcgct cggccgaggc cgagcccggg tcgctcttct tcgccgtccc
     2041 cgggggccgc tccgacgggc acgacttcgt tgcggacgcc gtggcgcgcg gggcggtcgc
     2101 cgcgctcgtc gagcatcccg tggcggcgaa ggtgacacag gtcgttgtgc cctcggtccg
     2161 ggtggcgatg gggcccgtgg ccgcggcgtt cttcggccgc cccgcggcgc gcctgacgct
     2221 cgtcggcgtc accgggacga acggcaagac gaccaccaca tacctcctgg aggcggtgtt
     2281 ccgggcggcg gggatgacgc ccggcgtgat cggcacaacg ggcgtgcgcg tcgacggcgg
     2341 gccgctgccg ctcgagcgga cgacgcccga ggcgcccgac ctccatcggc tgctgtaccg
     2401 gatggccgag gagggcgtcg gcgcggtggc gatggaggtc tcctcccacg gtctgcacca
     2461 gcatcgcgtt ggcgg
//
LOCUS       DASPWO010000158         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_445916, whole genome shotgun sequence.
ACCESSION   DASPWO010000158 DASPWO010000000
VERSION     DASPWO010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445916"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="VEO00_03600"
     CDS             complement(<1..1443)
                     /locus_tag="VEO00_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYU57120.1"
                     /translation="MADASTDALTLASPAAGADEARRLDDAVRRLAGRLAGLLAELEG
                     SGEGPAIGFLRDLALDDLGGWDGSCLDLPPGGAAHPVDRLAAALALEPADLDLLLLAG
                     APNQHEGLAWVLRRLHPAGEPWPTAGLVGALATAGLVPGLGSRTDARNRLTRGPLARP
                     GAVVVEGAGPFTERSVRCPSSLWGALNGDDAWPDAVRPILRPTPRWGLDGWLDEPPVA
                     AARRAVAVLDAVAVVATSDRPDATAARLGALVEAAGRTAALLAAATLDDRALTSVALH
                     AIAHGAVPVVAVEGRLDADLPVTDVPWPVVLSVPAGSRVTTWPRPQFEVPVGPLDLGS
                     RLAATAGALPELGEPLRPVGPATIEPSDLAIAAVDLRSAAALTGGTFSRTDLTRVVDD
                     RTTTSVPAGAVLVHPSADWDDLVLPPGRMAQLREAVHRVTGQGVVFDRWGFLEGRLGR
                     RGLRLLFAGPPGTGKTLAAEVVAGALGRDLL"
     gene            complement(1436..3013)
                     /locus_tag="VEO00_03605"
     CDS             complement(1436..3013)
                     /locus_tag="VEO00_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57121.1"
                     /translation="MSAPLVLDRFRITWSVKRVRPGDEDRRAGLVEALIDHGLEEAFE
                     RSGIRAGEEVCVRSVSAPPQTFDPGSDDETFVRRWAAAVAEAVVAAVASGSPGDDDRV
                     VRYRSRHAALGELVCRAAVGDERREWAWRQLGLWPDGLSPAEAVALALARSPHSIVGA
                     LEIAARSGALPSVFGLLGPDRLRTLVTAAWVAGEGEPAFVAMLDDRGGVPPSPADRAR
                     AGALLGATQLGSRLRAATAATPVGSATEPAMLAVLAAEPAIAARARFDPAAVALVRAV
                     RDELAGGRAAARWSKGDAAGDGGVRTSDRVDERSESPASPGVAHPQPDRPSGDPLDAV
                     DAGDDPSAPDGSAAPGRTAFGGLLFLLHLVDALDPVATSAADPVLAGVPLPRLLHRLG
                     TAILARATAAAPAEAPPDPRDPAVLAFCGLPPSAEPPGFEPPDPTWEDRVEAWAERIL
                     ALLRTALEPSVLASAPGDELLVAVCRRRAEVVADPGWIDVRLELDEVSTDVRRAGLDL
                     DLGYLSWLGCVVRFVYG"
     gene            complement(3010..>3448)
                     /locus_tag="VEO00_03610"
     CDS             complement(3010..>3448)
                     /locus_tag="VEO00_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57122.1"
                     /translation="GLLGDESAAASSSVVGFGFRVGGGVGRATAEPIAGTVRDAFETH
                     AHRFTLLVPCELDDEREQTTRHLLDMHRPAHTIVDLCTVGAGMRVGVGLHVELSSIVG
                     PSAGFRQARLGASRLGRGITVGRGTGGVRPSGTRVGVDSRVDT"
BASE COUNT          453 a         1336 c         1317 g          342 t
ORIGIN      
        1 gaggaggtcc cggccgagag cccccgcgac gacctcggcc gccagcgtct tgccggtccc
       61 cggcggcccg gcgaacagca ggcggaggcc gcgacgcccg agccgcccct cgaggaaccc
      121 ccaccggtcg aacacgacgc cctgcccggt cacccggtga acggcctccc gcagctgggc
      181 catgcggccg gggggcagca ccaggtcgtc ccagtccgcc gaggggtgaa cgagcacggc
      241 gccggccggg accgatgtcg tcgtgcggtc gtcgacgacg cgggtgaggt cggtgcggga
      301 gaacgttccc ccggtcaggg cggccgctga gcggaggtcg accgcggcga tcgcgaggtc
      361 gctcggttcg atcgtggccg ggccgacggg ccggagcggc tcgccgagct cggggagcgc
      421 cccggccgta gcggcgaggc gcgaaccgag gtcgagcggg cccacgggga cctcgaactg
      481 cggcctcggc catgtcgtga cccggctgcc ggcgggcacg gacagcacga cgggccacgg
      541 gacgtccgtg acgggaagat cggcgtcgag ccgcccctcg accgcgacca ccgggacggc
      601 tccgtgggcg atcgcgtgga gcgcaacgct cgtgagcgcg cggtcgtcga gggtggcggc
      661 cgcgagcagg gcggctgtcc ggcccgccgc ctcgaccagg gcccccaggc gggcagccgt
      721 ggcgtcgggg cgatcggagg tcgcgacgac ggcgacagcg tcgagcaccg cgacggcccg
      781 gcgggcggcg gccacggggg gctcgtcgag ccacccgtcg aggccccacc gaggcgtcgg
      841 ccgcaggatc ggccgcaccg catccggcca cgcgtcgtcg ccgttgagcg cgccccacag
      901 gctcgacgga catcggaccg accgctcggt gaacgggccg gcgccctcga cgacgaccgc
      961 gcccgggcgc gcgagcggcc cgcgagtcag gcggttcctc gcatcggtcc gggagccgag
     1021 ccccggaacc aggccggccg tcgccagcgc gccgacaagc ccggcggtcg gccagggctc
     1081 gccggcgggg tgcagccggc ggagcaccca cgccagtccc tcgtgctggt tcggcgcgcc
     1141 ggcaagcagg aggaggtcga ggtcggccgg ttccagcgcc agcgccgcgg cgaggcggtc
     1201 gaccgggtgt gcggccccgc cgggcggcag gtcgaggcag gacccgtccc acccgccgag
     1261 gtcgtcgagc gcgaggtccc ggaggaagcc gatcgccggg ccctcgccgc tcccctccag
     1321 ctcggcgagg agcccggcga gcctgcccgc gagccggcgg acggcgtcgt cgagccgccg
     1381 ggcctcgtcg gcgccggccg ccggcgacgc gagcgtgagc gcgtcggtcg atgcgtcagc
     1441 catagacgaa cctcacgacg cagccgagcc aggacaggta cccaaggtcg aggtcgaggc
     1501 cggcccggcg gacgtccgtg gagacctcgt cgagctcgag ccggacgtcg atccagcccg
     1561 ggtcggcgac gacctcggcc cgccgccggc acactgccac gagcagctcg tccccgggtg
     1621 cggacgcgag cacggacggt tcgagcgcgg tgcgcagcag cgcgaggatc cgttcggccc
     1681 acgcctcgac gcggtcctcc catgtcgggt ccgggggctc gaaccccgga ggttcggccg
     1741 agggagggag cccgcagaac gccagcaccg ccgggtcgcg cgggtcggga ggtgcctccg
     1801 ccggggcggc tgcggtcgcc ctggcgagga tcgccgtccc caggcggtgg aggagccgtg
     1861 gcagcggaac gccggccagc acggggtccg cggcggacgt ggccaccggg tcgagcgcgt
     1921 ccaccaagtg gagcaggaac aacagtccgc cgaaggcggt cctgccgggt gctgcgcttc
     1981 cgtcgggcgc ggacggatcg tcgccggcgt cgaccgcgtc gagcgggtcg ccggacggtc
     2041 ggtccggctg cgggtgcgcg accccggggg acgccgggga ttccgaacgc tcatcgaccc
     2101 gatcggatgt gcggacgcct ccgtcgcctg cggcgtcgcc tttcgaccat cgcgccgccg
     2161 cgcgcccgcc ggcaagctcg tcgcgaaccg cgcgaaccag ggcaaccgcg gcgggatcga
     2221 atcgcgcgcg cgcagcgatc gccggttccg cggcgaggac ggccagcatg gccggctcgg
     2281 tggccgaacc gaccggcgtc gccgccgtcg ctgcccggag ccgggagccg agctgcgtcg
     2341 cgcccagtag tgccccggcc cgtgcacgat ccgcgggcga aggaggcacc ccgccccggt
     2401 cgtcgagcat cgccacgaac gccggctcac cctccccggc gacccatgcg gccgtgacga
     2461 gggttcgcag gcggtccggc ccgagcaagc cgaacaccga cggcagcgcg cccgaccgag
     2521 cggcgatctc cagcgccccc acgatcgagt gcggcgaacg tgccagcgcg agcgcgactg
     2581 cctccgccgg ggagagcccg tccggccaga ggccgagctg gcgccacgcc cactcgcggc
     2641 gctcgtcgcc caccgccgcg cggcacacga gttcgccgag cgcggcgtgg cgcgaccggt
     2701 accggaccac ccggtcgtcg tcgccgggcg atccggacgc gaccgccgcc acgacggcct
     2761 ccgcgaccgc ggccgcccac cgccgcacga acgtctcgtc gtcggacccc gggtcgaacg
     2821 tctgcggcgg cgcggacacc gagcggacgc acacctcctc tccggcgcgg atgcccgacc
     2881 gttcgaaggc ctcctcgagc ccgtggtcga tcagggcctc gacgaggccg gcccgtcgat
     2941 cctcgtcgcc gggacgaacg cgcttgaccg accacgtgat gcggaaccgg tcgaggacga
     3001 gcggcgcgct cacgtgtcca ccctgctgtc gacgccgacg cgcgtccccg acggccggac
     3061 cccgcccgtg ccgcgcccga cggtgatccc cctccccagc cggctcgcgc cgagccgggc
     3121 ctgccggaac cccgcgctcg gcccgacgat gctcgacagc tcgacgtgga ggccgacgcc
     3181 gacccgcatc ccggccccca ccgtgcagag gtccacgatc gtgtgggccg gacggtgcat
     3241 gtcgagcagg tgccgggtgg tctgttcgcg ttcgtcgtcg agctcgcagg gcacgagcag
     3301 ggtgaatcgg tgcgcgtgcg tctcgaacgc gtcccggacg gtcccggcga tcggctcggc
     3361 ggtcgcccgc ccgacgccgc ccccgacccg gaacccgaac cccaccacgg agctcgacgc
     3421 ggccgcgctc tcgtcgccca gcaacccg
//
LOCUS       DASPWO010000159         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_194292, whole genome shotgun sequence.
ACCESSION   DASPWO010000159 DASPWO010000000
VERSION     DASPWO010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194292"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VEO00_03615"
     CDS             <1..1331
                     /locus_tag="VEO00_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYU57123.1"
                     /translation="DDQPPSSFPYAGTPLAMPWLEGRVADPADHWVNFSRAFVNEPVC
                     CPSRATLLSGRYAHHTGVTSNKNGDQFDESQTLATWLDGAGYRTGYIGKYLNGWPFDR
                     SPYTPQGWDRAVLFKGAKYYQYTLYVDGPLVPFGKEVADYSTDVLAGYALDFVRTAPT
                     DSPFFLVYAPLAPHYPHTPAQRHKTVFNDERQPPRPASLNEADVSDKPAWVRALPLLD
                     APTKQRYDQYRSSSYEAMIAVDEAMQQLVDAIDDRGQLSNTLILFMTDNGFLYGEHRT
                     KGKFSAYEESVRVPFAMRWEAAAPHTENRLVSNVDVASTVLAAAGVQPTLAQDGISLV
                     PLLGSSPPSSWRQDVLLEKRSGGVGDTGEKVPAYWGLRTKAYTYVEYDTGEVELYDMT
                     GALGPADPLQMKNRCPDPNTGDGVPAFRCVSPYASVQAQLSARLDELKAS"
     gene            1336..>3121
                     /locus_tag="VEO00_03620"
     CDS             1336..>3121
                     /locus_tag="VEO00_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYU57124.1"
                     /translation="MRRRVLLTAAAIAVVASLLSPAARATPVTISVNSTADPGTGTCD
                     QTECTLREAITQANNTPSTAETIAFSIGGAAPYVIAVGTPLPQLQGPVTIDGTTQPGY
                     AAPGPPLVEIDGNATFGSTSHGLEVVSGTATIRGLALYRFKQNGVTLRTSGNVVRENY
                     IGTNALLAGGLGNSLQGVKVNAVSGNRIIGNLITGNKAGGVRVSAPGTGNTITGNRIY
                     GNVDLEIDLLPAGVTPNDTGDGDTGANDLQNFPVLTEASSDGSTTTVSGTLNSKPSTA
                     YTLEFFASAACEPSGNGPAERSLGTKTVTTASGGNAPFTVSLTAGATPGERLTATATD
                     PADNTSELSACVIVEQPPGPPNVVIVMTDDQTPVSFPYTPAVLPYLQGRVLDPNDHWV
                     RFTEAYVNVPICCPSRATLLTGQYAHHHDVQGNNEGYKLDEDETLPVWLHREGYATAM
                     FGKYLNGFPFNRDPYKPRGWDRTALFDKEPGYTDYNLIEGPETGPVVVNHYGTGAANY
                     STDLLAARSVNFIDDYAGLQPFFLYYVPYAPHYPTKPNALHKTDFDDVRFTPRPANFN
                     EADVSDKPTWVQQLAVKSSTAISRMDNDR"
BASE COUNT          532 a         1137 c         1034 g          418 t
ORIGIN      
        1 gcgacgacca gcccccctcg tccttcccgt acgcggggac gccgctggcg atgccgtggc
       61 tggaagggcg cgtcgccgac ccggccgacc actgggtgaa cttctcccgc gcgttcgtca
      121 acgagccggt ctgctgcccc tcgcgggcga cgctgctctc ggggcggtac gcgcaccaca
      181 ccggcgtgac ctcgaacaag aacggcgacc agttcgacga gtcacagacg ctcgccacct
      241 ggctggacgg cgcgggctac cggacgggct acatcggcaa gtacctgaac ggctggccct
      301 tcgaccgcag cccctacact ccccagggct gggaccgggc ggttctgttc aaaggggcga
      361 agtactacca gtacaccctc tacgtcgacg ggccgctcgt gccgttcggg aaggaggtgg
      421 ccgactactc cacggacgtg ctggccggct acgccctgga cttcgtgcgc acagcgccga
      481 ccgacagccc gttcttcctg gtctacgcgc cgctcgcccc gcactatccg cacacgccgg
      541 cacagcgcca caagacggtc ttcaacgacg agcgccagcc gccgcggccg gcgagcctga
      601 acgaggcgga cgtgtccgac aagccggcct gggttcgcgc gctcccgctc ctcgacgcgc
      661 ccacgaagca gcgctacgac cagtaccggt cctcgtccta cgaggcgatg atcgcggtcg
      721 acgaggcgat gcagcagctc gtcgacgcga tcgacgaccg cggccagctg tcgaacaccc
      781 tgatcctctt catgaccgac aacgggttcc tgtacggcga gcaccggaca aaggggaagt
      841 tctccgcgta cgaggagagc gtgcgcgtcc cgttcgcgat gcgctgggag gcggccgcgc
      901 cgcacacgga gaatcgcctg gtgtcgaacg tcgacgtcgc gtcgacggtc ctcgccgccg
      961 ccggcgtcca gccgacgctc gcgcaggacg ggatcagcct ggtgccgctg ctcggatcct
     1021 cgcccccttc ctcctggcgc caggacgtcc tgctcgagaa gcgctccggc ggcgtgggtg
     1081 acaccggcga gaaggtcccg gcatactggg ggctccgcac caaggcctac acgtacgtcg
     1141 agtacgacac cggagaggtg gagctgtacg acatgaccgg cgcgctcggg cccgcggatc
     1201 cgctccagat gaagaaccgg tgtcccgacc ccaacacggg cgacggcgtg ccggcgttcc
     1261 ggtgcgtgtc cccctacgcg tcggtgcagg cgcagctgtc ggcccgcctg gacgagctga
     1321 aggcgagttg accgggtgcg acggcgcgtc ctgctgaccg ccgcggcgat cgccgtcgtg
     1381 gcgtcgctgc tctcacccgc ggcgcgggcc acgccggtca cgatcagcgt caactccacg
     1441 gccgaccctg gcacgggcac ctgcgatcag acggagtgca cgctgcgcga ggcgatcacg
     1501 caggcgaaca acactccctc gacggccgag acgatcgcct tctcgatcgg cggggccgcg
     1561 ccgtacgtca tcgcggtggg cacgccgctg ccccagctgc agggcccggt gacgatcgac
     1621 ggcacgaccc agcccggcta cgcggcaccg gggcccccgc tcgtggagat cgacgggaac
     1681 gcgaccttcg gctcgacctc ccacggcctc gaggtggtct ccgggacggc gacgatccgc
     1741 gggctcgcgc tctaccgttt caaacagaac ggggtgacgc tgcggacgtc ggggaacgtc
     1801 gtgcgggaga actacatcgg cacgaacgcg ctgctggcgg gcgggctcgg gaactccctg
     1861 cagggggtca aggtcaacgc agtgagcggc aaccggatca tcggaaacct gatcaccggc
     1921 aacaaggcgg gcggtgtccg ggtctcggcg cccggtaccg ggaacaccat cacgggcaac
     1981 cggatctacg ggaacgtgga cctcgagatc gacctgctgc ccgcgggcgt gacaccgaac
     2041 gacaccggcg acggggacac cggggcgaac gacctgcaga acttccccgt gctcaccgag
     2101 gcgtccagcg acggttcgac gacgacggtc tccgggacgc tgaacagcaa gccgtcgacc
     2161 gcttacacac tggagttctt cgcctcggcg gcctgtgagc cgtccggcaa cggaccggct
     2221 gagcgctccc tggggacgaa gacggtgacc accgcgtcgg ggggcaacgc cccgttcacg
     2281 gtgtcgctaa cggcgggggc gacgccgggc gagcggctga ccgccaccgc gaccgacccg
     2341 gccgacaaca cgtccgagct gtcagcctgc gtgatcgtcg agcagccgcc cggtccgccg
     2401 aacgtggtga tcgtgatgac cgacgaccag acgccggtct cgttcccgta cacgcccgcc
     2461 gtgctcccct acctgcaggg ccgcgtgctc gacccgaacg accactgggt ccggttcacc
     2521 gaggcctacg tcaacgtgcc gatctgctgt ccctcgcggg ccaccctgct caccgggcag
     2581 tacgcgcacc accacgacgt gcaggggaac aacgagggct acaagctcga cgaggacgag
     2641 acgctcccgg tctggctgca ccgcgagggc tacgcgaccg ccatgttcgg caagtacctg
     2701 aacgggttcc cgttcaaccg cgacccgtac aagccgcggg gctgggatcg caccgccctg
     2761 ttcgacaagg aacccggcta cacggactac aacctgatcg agggccccga gaccgggccg
     2821 gtggtggtca accactacgg caccggcgcg gcgaactact cgaccgacct gctggcggcc
     2881 cgctcggtga acttcatcga cgactacgcc gggctgcagc cgttcttcct ctactacgtc
     2941 ccgtacgccc cgcactaccc gacgaagccc aacgccctgc acaagacgga cttcgatgac
     3001 gtccgcttca cgccgcggcc ggcgaacttc aacgaggccg acgtctcgga caagccgacc
     3061 tgggtgcagc agctggccgt gaagagcagc acggccatca gccggatgga caacgaccgc
     3121 c
//
LOCUS       DASPWO010000160         6645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1209185, whole genome shotgun sequence.
ACCESSION   DASPWO010000160 DASPWO010000000
VERSION     DASPWO010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209185"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VEO00_03625"
     CDS             complement(<1..719)
                     /locus_tag="VEO00_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HYU57125.1"
                     /translation="MRGRLVGAVTAVGLAAALLAPLSGHLAAAAERAGPAPAARSDFD
                     GDGFADLAVGVRFEDVGAVTGAGAVTIIGGSASGLGGDMTTWSQDSPGVGGKAATSDF
                     FSSALATGDFDGDGFADLAIGAQLDDPAGVEDTGAVNVLYGSAEGLTATGSQYWYQDS
                     PAVVEAAEAGDHFGGALAAGDFDGDGLDDLAVGVEDEDVGVVQDAGAVIVLRGTSGGL
                     TGTGSRLLHQDRSGVPGQAAQR"
     gene            complement(752..2704)
                     /locus_tag="VEO00_03630"
     CDS             complement(752..2704)
                     /locus_tag="VEO00_03630"
                     /inference="COORDINATES: protein motif:HMM:NF039734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6345 domain-containing protein"
                     /protein_id="HYU57126.1"
                     /translation="MRTPRRVALLLSFLLIVGLAIELPAARGAELQLPIFLVKATGPT
                     QQQAGALANQLNLLGNPRNRFGGLVYMAPNYGNLPTRQLEGGGDPDEGEHKITHTGLN
                     LPAIQKIQVPSGEEVAQRVGAALGGAGLDPAKFLMADGSVRPSIGHSTFTFQRPQDEA
                     SSPPVPITTELNFRFSLNAGTPDKPQMVPLVGPGAQVKVVENPQGQVTALQYELRRLS
                     RGPMVNIVDEQTALDRCGLALGQRVAAAALSVKLVYYAPPLEPTRAMPFPGSIGRVSP
                     WYECDGSVLIGDDTVRARSVFVPAAVGSLHAQAQLSITKNTSAEAAAVVSGGTQPYSF
                     YWSSSTGSISPAQADDGSLIDYEVMPKEEGFPPVETACVLVTDANGLTSTACDSAQID
                     GTAPAGPPPAPSRSRIVGRVDFGTEYLGTSAHLIGLADLSGSAPNAGGFDSRMRSSAT
                     SQFFWAETNAWERDFKQPGIGGGQDSSYVDDVDVVFYTGHANENGFSFSSNRDDNQLR
                     YSDAQWGDRDLEWIALAACLILNRASVGGLDWAGRWGGSFQGLHMILSYATVSSDNTI
                     EGDHFADYMTRTPFLWWNNPMKVRDAWVQTAIDSQPSTVTWAFMGPLGRNGLSDYDDF
                     FWNKGATGPDIRGADLTGWYQVQGPS"
     gene            2811..3836
                     /gene="mgrA"
                     /locus_tag="VEO00_03635"
     CDS             2811..3836
                     /gene="mgrA"
                     /locus_tag="VEO00_03635"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HYU57127.1"
                     /translation="MSYVAADMRYESMQYRRCGRSGLRLPAVSLGLWHNFGHDRPIDT
                     QREILRRAFDLGITHFDLANNYGPPYGSAEENFGRILASDLLPYRDELVVSTKAGYDM
                     WPGPYGEWGSRKYLLASLDQSLRRMGLDYVDIFYSHRFDPETPLEETMGALDSAVKSG
                     KALYAGISSYSAERTREAATILRDLGTPLLIHQPSYSMLNRWIEPDLLDVLGSEGVGC
                     ITFSPLAQGLLTDRYLDGIPEDSRVARGGALSKATLTEENLAKVRALNDVAKRRGQTL
                     AQTAVAWTLRDPRVTSTLIGASSVAQLEDNVAALDHLEFADEELREIDAHATDAGINL
                     WQRSSAG"
     gene            complement(3845..4258)
                     /gene="arfB"
                     /locus_tag="VEO00_03640"
     CDS             complement(3845..4258)
                     /gene="arfB"
                     /locus_tag="VEO00_03640"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HYU57128.1"
                     /translation="MDGDLVTAGGIVVPANAMTWRFSRGTGPGGQGVNTTDSRVELVV
                     DLATLVASDEVAARVRRALGDTLRIVAATERSQLRNREAALRRLGERLDAAARPRRPR
                     APTRPTAGAVRRRLEAKRRRARIKAHRRDPTADAD"
     gene            4364..5044
                     /locus_tag="VEO00_03645"
     CDS             4364..5044
                     /locus_tag="VEO00_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYU57129.1"
                     /translation="MGTRRKRTEPIARHFDRKADCCAVDATPEAPAKLIRVSRLMLEA
                     LAGAGLEGSSVLDLGCGQGPFSLAVLEHGAERVTGIDLSPMAIEVARARAAAAGLGDR
                     ARFLVGDAAAEDVPAHDVVVLNRVICCYPDVDDLLRTSLAATGRVYGVIVPASSGLWG
                     LLSRMALGFENLLRVVRRDPFRAYVHPVERVDQAAAGAGLVPTRRSRRRIWRLAVYVR
                     PTGATATS"
     gene            complement(5164..6026)
                     /locus_tag="VEO00_03650"
                     /pseudo
     CDS             complement(5164..6026)
                     /locus_tag="VEO00_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
     gene            6164..6364
                     /locus_tag="VEO00_03655"
     CDS             6164..6364
                     /locus_tag="VEO00_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57130.1"
                     /translation="MVNDVRAAFRFSGGLIADHVDSFSLSRWARQALGPTGLVLGWTT
                     MVRNGVRRKARAGLEKFMSTEG"
     gene            complement(6386..6580)
                     /locus_tag="VEO00_03660"
     CDS             complement(6386..6580)
                     /locus_tag="VEO00_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57131.1"
                     /translation="MENRTVPARTRLTERARKARAKVIAAHQSARTARRAADEAAEVQ
                     QAKDRARLEMLEARNAPRSD"
BASE COUNT          923 a         2402 c         2332 g          988 t
ORIGIN      
        1 cgctgggcgg cctggccggg cacgccggac cggtcctggt gcaggagccg ggagccggtg
       61 ccggtcaacc ctccggacgt ccctcggagc acgatcacgg cccccgcatc ctgcacgacc
      121 cccacgtcct catcctcgac gcccaccgcg agatcgtcga gcccgtcccc gtcgaagtcg
      181 cctgccgcca gcgccccgcc gaagtggtcg cccgcctccg ccgcttccac gacggccgga
      241 ctgtcctgat accagtactg gctcccggtc gccgtgaggc cctcggcgga cccgtacagc
      301 acgttgaccg ctcccgtgtc ctcgaccccc gccggatcgt cgagctgggc cccgatggcg
      361 aggtcggcga acccgtcgcc gtcgaagtcg cccgtggcga gcgcgctcga gaagaagtcg
      421 ctggtggccg ccttgccgcc gacccccgga ctgtcctggc tccacgtcgt catgtcgccg
      481 ccgaggccgg acgcggagcc cccgatgatc gtgaccgccc ccgcgccggt gaccgctccc
      541 acgtcctcga atcggacccc gacggccagg tcggcgaatc cgtcgccgtc gaagtcgctc
      601 cgcgcggcgg gcgccggacc cgcccgttcg gccgcggcgg cgaggtggcc ggagagcggg
      661 gcgagcagcg cggccgccag tcccaccgcc gtcacggcgc cgaccagacg acctctcacc
      721 acgtgccccc gtcgtcgttc gagcgatcag cctacgacgg gccctggacc tggtaccagc
      781 cggtgaggtc ggcgcctctg atgtccggtc ccgtggcccc cttgttccag aagaagtcgt
      841 cgtagtcgct gaggccgttg cgtcccaacg ggcccatgaa cgcccaggtc acggtggacg
      901 gctgggagtc gatggcggtc tggacccagg cgtcccggac cttcatgggg ttgttccacc
      961 acaggaacgg cgttcgggtc atgtagtcgg cgaagtggtc gccctcgatc gtgttgtcgc
     1021 tgctcacggt ggcgtaggac aggatcatgt gcaggccctg gaacgagccg ccccagcggc
     1081 cggcccagtc cagcccgccc acggaggcgc ggttcaggat caggcacgcg gcgagggcga
     1141 tccactcgag gtcccggtcc ccccactgcg cgtcgctgta ccgcagctgg ttgtcgtcgc
     1201 ggttgctgct gaacgagaac ccgttctcgt tcgcgtggcc ggtgtagaag acgacgtcga
     1261 cgtcgtccac gtaggacgag tcctggccgc ccccgatccc cggctgcttg aagtcgcgtt
     1321 cccacgcgtt cgtctcggcc cagaagaact gcgaggtcgc ggacgaacgc atccggctgt
     1381 cgaacccgcc cgcgttcggg gccgacccgc tgaggtcggc cagcccgatc aggtgcgcgc
     1441 tggttcccag gtactcggtc ccgaagtcga cccggccgac gatgcgcgac cgcgagggcg
     1501 ccgggggcgg accggccggc gccgtgccat cgatctgcgc cgagtcgcac gccgtggacg
     1561 tcaggccgtt cgcgtcggtg accagcacgc aggccgtctc gacgggcggg aacccctcct
     1621 ccttcggcat gacctcgtag tcgatgagcg agccgtcgtc ggcctgggcc ggggagatcg
     1681 accccgtcga gctgctccag tagaacgagt acggctgcgt ccctcccgac accacggctg
     1741 ccgcctccgc cgacgtgttc ttggtgatgc tcagctgggc ctgcgcgtgg aggctgccca
     1801 ccgccgcggg aacgaacacg gatcgcgccc gcacggtgtc gtcgccgatg agcaccgacc
     1861 cgtcgcactc gtaccacggt gagacccgcc cgatcgagcc ggggaacggc atggcacgcg
     1921 tcggctcgag cggcggcgcg tagtacacga gcttcacgga gagcgccgca gcggcgaccc
     1981 gctgtccgag cgcgaggcca caccggtcga gcgccgtctg ctcgtccacg atgttcacca
     2041 tcgggccccg ggacagccgg cgcagctcgt actgcagcgc ggtgacctgg ccctgtgggt
     2101 tctcgaccac cttcacctgc gcgcccggcc cgaccagtgg caccatctgc ggcttgtccg
     2161 gcgtccccgc gttcagcgag aaccggaagt tcagctcggt ggtgatcggg acgggcggcg
     2221 acgaggcctc gtcctggggt cgctggaacg tgaacgtgga atgcccgatc gagggccgca
     2281 ccgagccgtc ggccatcagg aacttcgccg ggtccagccc ggcgccgccg agcgcggcgc
     2341 cgaccctctg cgccacctcc tcgccgctcg gcacctggat cttctggatg gctgggaggt
     2401 tgagccccgt gtgcgtgatc ttgtgttcgc cctcgtcggg atcgccgccg ccctcgagct
     2461 gccgcgtcgg gaggttcccg tagttcggag ccatgtacac caggccgccg aaccggttcc
     2521 gcgggttccc caacaggttg agctgattcg ccagcgcccc ggcctgctgt tgcgtgggac
     2581 cggtggcttt gacgaggaag atcggcagct gcagctcggc cccgcgggcc gccggcagct
     2641 cgatcgcgag gccgacgatc agcaggaacg acaggagcag agcgacgcga cgcggggtcc
     2701 tcatggttcc ctccctggga gattgctcgg gccacgagga ttctaggccg ggccgcgagg
     2761 gcaagtgggc cgaatggccg tccgccctcc cgggtggcac actcgcgggc atgtcgtatg
     2821 tggcggcgga catgcggtac gagtcgatgc aataccggcg gtgtggtcgc tccgggctgc
     2881 ggttgccggc cgtgtcgctc gggttgtggc acaacttcgg gcacgaccgc cccatcgaca
     2941 cgcagcgcga gatcctgcgg cgggcgttcg acctggggat cacgcacttc gacctggcga
     3001 acaactacgg gccgccgtac ggctcggccg aggagaactt cgggcggatc ctggcgagcg
     3061 acctcctccc gtaccgcgac gagctggtcg tctccacgaa ggccgggtac gacatgtggc
     3121 cgggaccgta cggggagtgg gggtcgcgga agtacctgct ggcgagcctc gaccagtcgc
     3181 tccggcgcat ggggctcgac tacgtcgaca tcttctactc gcaccggttc gatcctgaga
     3241 cgccgctcga ggagaccatg ggggcgctcg actcggccgt gaagagcggg aaggcgctgt
     3301 acgccgggat ctcgtcctac tcggccgaac ggacccggga ggccgccacg atcctccggg
     3361 acctcgggac gccgctcctc atccaccagc cctcctattc gatgctgaac cggtggatcg
     3421 aacccgacct gctggacgtc ctgggctccg agggcgtcgg ctgcatcacg ttctcgccgc
     3481 tcgcgcaggg gctcctcacg gaccgctacc tggacgggat cccggaggac tccagggtgg
     3541 cgaggggcgg cgcgctatcg aaggcgacgc tcaccgagga gaacctggcc aaggtccggg
     3601 ccctgaacga cgtcgcgaag cggcgcgggc agacgctcgc ccagacggcg gtggcctgga
     3661 cgctccgcga ccccagggtc acctccacgt tgatcggcgc ctccagcgtg gcccagctcg
     3721 aggacaacgt cgccgcgctg gaccacctgg agttcgcgga cgaggagctg cgggagatcg
     3781 acgcccacgc caccgacgcc ggcatcaacc tctggcagcg ctcctctgcc ggctgacccg
     3841 ccgatcagtc cgcgtcggcg gtggggtcgc ggcggtgggc cttgatccgg gcgcgccggc
     3901 gcttggcctc gagccgcctc cgcaccgcgc cggcggtggg acgggtcggg gcgcggggcc
     3961 ggcggggccg agcggccgcg tcgagccgtt cgcccagccg ccggagcgcc gcctcccgat
     4021 tgcgcagctg cgatcgctcg gtggcggcga cgatccgcag cgtgtcgccg agcgcccgtc
     4081 gcacacgagc ggcgacctcg tccgaggcga ccagcgtggc gagatccacc accagctcca
     4141 cgcggctgtc ggtggtgttg accccctggc cgcccggccc cgtgccgcga gagaaccgcc
     4201 acgtcatggc gttcgccgga accacgatgc ctcccgcggt gaccaggtcc ccgtccatac
     4261 ccgacagtgt gcccggtcgg cgaacgtgtt ccacggtttg gccggccatc tccggatcga
     4321 ggagactgcg aggcaacagc tgttcgaaat cgggggtgaa cacgtgggaa cgcgacggaa
     4381 acgcaccgag cccatcgcca ggcacttcga ccggaaggcg gactgctgcg cagtggacgc
     4441 gacaccggag gccccggcca agctgatccg ggtcagtcgc ctgatgctgg aggcgctggc
     4501 gggggccggg ctcgagggaa gctccgtgct cgacctcgga tgcgggcagg gccccttctc
     4561 gctcgcggtc ctggagcacg gggccgaacg ggtcaccggc atcgatctct ccccgatggc
     4621 gatcgaggtc gcccgggcgc gcgcggcggc ggccggcctg ggcgaccggg cgcggttcct
     4681 ggtcggcgac gccgcggccg aggacgtccc ggcgcacgac gtcgtggtcc tcaaccgggt
     4741 gatctgctgc tacccggacg tcgacgacct gctccgaacg agcctcgccg cgacgggccg
     4801 cgtctacgga gtgatcgtcc ccgcgtcctc gggcctgtgg ggactcctct ccaggatggc
     4861 cctcgggttc gagaacctcc tgcgcgtcgt ccgccgggat ccatttcggg cctacgtcca
     4921 cccggtcgaa cgcgtcgacc aggcggcggc cggagccggt ctggttccga cccgacgcag
     4981 ccgacgccgc atctggcgcc tcgccgtcta cgtccgcccc accggcgcga ccgccacctc
     5041 ctagtgtcgt gtgaatgaag tagctttgct tcgcggcagc atcgtgtatg tcgctgactg
     5101 ctgccgtgga attgccggcc gaggaccgcg tgcgatgaca ggattcccga agaccttggt
     5161 tggctactcg ggtggggatc tccgaaatgc gtcggcgtgc tcggagaccc actgacgaaa
     5221 cgtccgcgcc ggcgctccga cgacgtcggc cacggttgaa gtcacgaacg ccgggcgtcc
     5281 gatcgtcgca ccccacgcgt cgagcagcat ctccacgacc ggacccggcc aggtacccgc
     5341 cgtctcacgc cggaactctt cgggcgacag ctcctcgaac cggatctggc gaccgaggac
     5401 gctcccgatc acgctcacct gctccgcctg gctcagcgac tcggggccgg tgaggacgta
     5461 atcgccgccc gtgtgccctt cctcatacag ggcgcgcgcc gccacggccg cgacgtcgcg
     5521 ctcgtcgatc ggtgcggtct cggctgcacc gtagggccac cggacgacgt cgccctcacg
     5581 gatcgcgggg ccccaccaga actgcgcgtt cgacgcgaac atcccgggcc ggatgaacgt
     5641 cgactcgagt ccggcggccg cgatcaggcg ctcaatctcg gcgtgcaagc gagccatggg
     5701 gttcggctgc tggaagaacg ggtgcggtgt ctggtgtggc gaggagagaa agacgacccg
     5761 ccgcgtgtcc gacgcgagac gctcgacgac ggccgcagcg gtggcgagcg gcgcggtcca
     5821 gaggaggaac accgcacgcc ctgcagcgcg gggtcgaggg agtcgggctc ggtgaagtcg
     5881 cccgccacga cctcggcgat cgccggcagc gcggccgtcg caggtttgcg cgtgagcgcg
     5941 cgcaccggca cgcccgcgcc caggagctcg gcgatcaccg cgcgcccgac tcggccagtg
     6001 gctcccgtca ccaggacggg ttgcatgttg acccgctcgt tgtacttccg ttgcgcgttc
     6061 ccggtcacca ggacctcccg aacctccttc tgacccggcc gtggcgtaga aagcgtccgt
     6121 acggtggatc gccgactaca cgttcacgca gaccgggcgg cacgtggtga acgacgtccg
     6181 ggccgcgttc cggttctcgg gcgggctgat cgccgaccac gtggattcgt tcagcctctc
     6241 gcggtgggcg cggcaggcgc tcgggccgac ggggctggtc ctcgggtgga cgacgatggt
     6301 ccggaacggc gttcgccgca aggccagagc gggcctcgag aagttcatgt cgacggaggg
     6361 ctgacccccg gcccgactcc gatcgtcagt cggagcgcgg cgcgttccgc gcttcgagca
     6421 tctcgaggcg ggctcggtcc ttggcctgct ggacctcagc ggcctcgtcg gccgcacgcc
     6481 gcgccgttcg cgcggactgg tgggccgcaa tgaccttggc acgcgctttc cgggcgcgct
     6541 cggtgaggcg cgtcctggca gggacggttc tgttctccac ttcgacggcg cctctcttcc
     6601 ggggaccctc agcctactcc cctttcgggg cccgccgggg ccccc
//
LOCUS       DASPWO010000161         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_280199, whole genome shotgun sequence.
ACCESSION   DASPWO010000161 DASPWO010000000
VERSION     DASPWO010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280199"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /gene="groL"
                     /locus_tag="VEO00_03665"
     CDS             complement(<1..1350)
                     /gene="groL"
                     /locus_tag="VEO00_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180551.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HYU57132.1"
                     /translation="MSKIITFHEEARRALERGMDQLANAVRITLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPEEKIGAELVKEVAKKTDDVAGDGTTTATVLAQAMVKEG
                     LRNVAAGANPMALKRGIEAAVDATVEAIMKQAKDVEEKDDIAHVASISAGDPEIGAEI
                     AEAMDKVGKDGVITVEESQTFGMELELVEGMRFDKGYISPYFVTDPERMEAVIEDPYI
                     LLVNAKISAVKDLLPVLEKVMQSGKPLVVLAEDVEGEALATLVVNKIRGTFKSVAVKA
                     PGFGDRRKAMLQDMAILAGGQVVSEEVGLKLENTNLDMLGSARKVVVTKDETTIVEGG
                     GSPEEIKGRINQIKGEIDKTDSDYDREKLQERLAKLSGGVAVIKVGAATEVELKEKKH
                     RIEDAVQTTKAAVEEGIVPGGGVALLGAQEALTKLDLTGDEATGAALVRKALEEPIKQ
                     "
     gene            complement(1468..>2241)
                     /locus_tag="VEO00_03670"
     CDS             complement(1468..>2241)
                     /locus_tag="VEO00_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYU57133.1"
                     /translation="EAWLWYHRVIGGERCPVVDTWWQTETGAILITPLPGITPLKPGS
                     ATRPFPGIEADVYDEQGRPVPPGRGGFLVLKRPWPAMFRTIHGDPERYVETYFARYGA
                     DTYLAGDGAKRDADGFFWLLGRIDDVMNCAGHRISTYEVESALVDNPRVAEAAVVGRT
                     DPEIGQAIVAYVTLRGGEQPSDAVAAELREHVAKVIGKIARPHSIVFTDDLPKTRSGK
                     IMRRLLRDVAEGRTLGDVTTLANAEIVEQIRESAAAGEE"
BASE COUNT          283 a          836 c          707 g          415 t
ORIGIN      
        1 ctgcttgatc ggctcctcga gcgccttgcg cacgagcgcg gcgccggtgg cctcgtcgcc
       61 ggtcaggtcg agcttggtca acgcctcctg ggctccgagc agcgccacgc cgccgcccgg
      121 gacgatgccc tcctcgaccg cggccttcgt cgtctggacg gcgtcctcga tgcggtgctt
      181 cttctccttc agctccacct cggtggccgc gccgaccttg atcaccgcga cgccgccgga
      241 cagcttcgcc agccgctcct gcagcttctc acggtcgtag tcggagtcgg tcttgtcgat
      301 ctcccccttg atctggttga tacggccctt gatctcctcg gggctgccgc cgccctcgac
      361 gatcgtcgtc tcgtccttcg tcacgacgac cttgcgggca gagccgagca tgtcgaggtt
      421 ggtgttctcg agcttcaggc cgacctcctc ggagaccacc tgccctccgg ccaggatcgc
      481 catgtcctgc agcatggcct tgcggcggtc cccgaagccg ggggccttca ccgcgacgct
      541 cttgaacgtg ccgcggatct tgttcaccac gagcgtggcg agcgcctcgc cctcgacgtc
      601 ctcggcgagg accacgagcg gcttgccgga ctgcatgacc ttctccagca ccggcagcag
      661 gtccttcacc gccgagatct tcgcgttcac gagcaggatg taggggtcct cgatgaccgc
      721 ctccatccgc tcggggtcgg tgacgaagta cggcgagatg tagcccttgt cgaagcgcat
      781 cccctcgacg agctcgagct ccatcccgaa ggtctgcgac tcctcgaccg tgatcacgcc
      841 gtccttccca accttgtcca tggcctcggc gatctcggcc ccgatctccg ggtcaccggc
      901 ggagatcgac gcgacgtgag cgatgtcgtc cttctcctcg acgtccttgg cctgcttcat
      961 gatcgcctcg acggtcgcgt cgacggcggc ctcgatgccc cgcttgaggg ccatcgggtt
     1021 ggcgccggcc gcgacattgc gcaagccttc cttgaccatc gcctgcgcga gcaccgtggc
     1081 ggtggtggtg ccgtcgccgg ccacgtcgtc ggtcttcttc gcgacctcct tgaccagctc
     1141 cgccccgatc ttctcctcgg ggtcctccag ctcgatctcc ttggcgatcg atacgccgtc
     1201 gttggtgatc gtcggggcgc cccacttctt ctcgaggacg acgttgcggc ccttcggccc
     1261 gagcgtgatc cggaccgcgt tcgccagctg gtccatcccc cgctccagcg ctcgccgcgc
     1321 ctcctcatgg aacgtgatga tcttcgacat ctcggctcct cctcgaggtc ggcctgcccg
     1381 ttacccgttt gagttagcac tctcgcaccg ggagtgctaa ccgcgattat aggacgcgag
     1441 ccgcggtcgt tggcaaactc cccggcgcta ctcctcgccg gccgccgcgg actcccggat
     1501 ctgctcgacg atctcggcgt tggcgagcgt ggtgacgtca ccgagcgtgc ggccctcggc
     1561 aacgtcgcgc aggagccggc gcatgatctt gcccgagcgc gtcttcggca ggtcgtcggt
     1621 gaacacgatc gagtgcggcc gggcgatctt cccgatcacc ttcgccacgt gctcgcgcag
     1681 ctccgccgcc acggcgtccg agggctgctc gccgccccgc agcgtgacgt aggccacgat
     1741 ggcctggccg atctcggggt cggtccgccc gacgacggcc gcctcggcga cccgcgggtt
     1801 gtcgaccagg gccgattcca cctcgtacgt cgagatgcgg tggccggcgc agttcatcac
     1861 gtcgtcgatc cgcccgagca gccagaagaa gccgtccgcg tcgcgcttgg cgccgtcccc
     1921 ggcgaggtac gtgtcggcgc cgtagcgcgc gaagtaggtc tcgacgtagc gctccgggtc
     1981 gccgtggatc gtgcggaaca tggcaggcca cggccgcttc agcaccagga acccgccgcg
     2041 ccccggcggc accgggcggc cttgctcgtc gtaaacgtcg gcttcgatcc ccgggaaggg
     2101 gcgcgtcgcc gacccgggct tcagcggcgt gatcccgggg agcggcgtga tcaggatcgc
     2161 gccggtctcg gtctgccacc aggtgtccac caccgggcag cgctcgccac cgatcacccg
     2221 gtggtaccac agccacgcct c
//
LOCUS       DASPWO010000162         2304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_194648, whole genome shotgun sequence.
ACCESSION   DASPWO010000162 DASPWO010000000
VERSION     DASPWO010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194648"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /gene="zwf"
                     /locus_tag="VEO00_03675"
     CDS             <1..1445
                     /gene="zwf"
                     /locus_tag="VEO00_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HYU57134.1"
                     /translation="ERVAAKDLPAQLVVIFGASGDLTQRKLLPAFYHLHCEGLLPDGF
                     TIVGYARTEMGSEGFVERARQSIAEHGRSAPEGERWAEFSRLLRYESGDFASEHDFDD
                     LEALLAGVDRERGSAAGRFYYCATPPQAVPLIVDRIGESGMAENSRIVIEKPFGRDLE
                     SARELNRKLHEVFLEKQIFRIDHYLGKETVQNILALRFSNGMFEPIWNRRYVDNVQIT
                     VAEEIGVEGRGAFYDQTGALRDMVSTHLFQVLTFVAMEPPVSFEPERLRDEKVRVLRS
                     MPLLRPERVVLGQYEGYWDERDVALDSGTETFAALQVEVDNWRWAGVPFNLRTGKRLE
                     RKVTEVSLSFRDVPYNVFRGSEAVPPGRDALTIRVQPNEGLDLHLNIKRPGFRMELDR
                     AVLGFDYDEMYWRPLVEAYETLLLEVMEGDHTLFTREDEVERAWEVLGSVLDEPPPAI
                     AYPPGSWGPDEAAELLLPHHWHVTPVRPGP"
     gene            1566..2183
                     /locus_tag="VEO00_03680"
     CDS             1566..2183
                     /locus_tag="VEO00_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYU57135.1"
                     /translation="MRVKEIMTSPVVTVRPETTVKEAATLLVQRGIDAVPVVGEDGTL
                     AGIVSESDLLPLELRGDPRSEILAAPPYARDHSPETVAEVMTARVISLPEDADVAEAA
                     RRMLENRVNQIPITRDDQVVGIVARRDLLKVVAREDEAIRGELLDLLREGDGLLGPYD
                     VDVVEGVALLRGGRDRSSRRLADILARSVPGVIEVRFEDAGTGSE"
BASE COUNT          336 a          763 c          869 g          336 t
ORIGIN      
        1 aggagcgcgt ggcggcgaag gacctgccgg cgcagctggt ggtgatcttc ggcgcgtccg
       61 gggacctgac gcagcggaag ctgctgcccg ccttctacca cctgcactgc gagggcctgc
      121 tgccggacgg gttcaccatc gtcggctacg cgcggaccga gatggggagc gaggggttcg
      181 tcgaacgggc acggcagtcg atcgccgagc acggccgctc ggccccggag ggcgagcgct
      241 gggcggagtt ctcccggctg ctccgctacg agtccggtga cttcgccagc gagcacgact
      301 tcgacgatct tgaggcgctg ctcgccgggg tcgatcgcga gcgagggtcc gcggccgggc
      361 ggttctacta ctgcgcgacg ccgccccagg ccgtccccct gatcgtcgac cgcatcggcg
      421 agtctgggat ggccgagaac agccggatcg tcatcgagaa gccgttcggc cgcgacctgg
      481 agagcgcccg cgagctgaac cgcaagctgc acgaggtgtt cctcgagaaa cagatcttcc
      541 gtatcgacca ctacctggga aaggagaccg tgcagaacat cctggcgctg cgcttctcga
      601 acgggatgtt cgagccgatc tggaaccggc gctacgtgga caacgtgcag atcacggtgg
      661 ccgaggagat cggggtcgag gggcgcggcg ccttctacga ccagaccggg gcgctgcgcg
      721 acatggtctc gacccacctg ttccaggtgc tgacgttcgt ggcgatggag ccgccggtgt
      781 cgttcgagcc ggagcgcctg cgcgacgaga aggtcagggt gctgcgctcg atgcccctgc
      841 tgcgcccgga gcgggtggtg ctcggacagt acgagggcta ctgggacgag cgggacgtcg
      901 ccctcgactc ggggaccgag accttcgcag cattgcaggt cgaggtggac aactggcgct
      961 gggcgggggt cccgttcaac ctacgcaccg gcaagcgcct ggagcggaag gtgaccgagg
     1021 tctcgctgtc gttccgcgac gtcccctaca acgtcttccg cgggagcgag gccgtgccgc
     1081 ccggccgcga cgccctgacc atccgggtgc agccgaacga ggggctggac ctgcacctga
     1141 acatcaagcg ccccggcttc aggatggagc tcgaccgcgc ggtgctgggg ttcgactacg
     1201 acgagatgta ctggcggccg ctggtcgagg cgtacgagac gctgttgctc gaggtgatgg
     1261 agggcgacca caccctgttc acccgggagg acgaggtcga gcgagcctgg gaggtgctgg
     1321 gctccgtgct cgatgagccc ccaccggcga tcgcctaccc gcccggcagc tggggtccgg
     1381 acgaagcagc cgagctgctc ctgccacatc actggcacgt caccccggtc cgtccgggac
     1441 cctaggcgca ccagggaggc tgcccgtttg tcccgcgcgc gccgggccgg gtggtcgtct
     1501 cgaatgtctg ccgggcgggc gtacggtacg gatggcgggg ttcgtcccgc ctggagggtc
     1561 gtgccatgcg agtgaaggag atcatgacgt ccccggtggt caccgtgagg ccggagacca
     1621 ccgtgaagga agcggcgacg ctcctcgtcc agcgcggcat cgacgcggtg ccggtggtcg
     1681 gcgaggacgg gacgctcgcc gggatcgtct cggagagcga cctgctgccg ctggagctgc
     1741 ggggcgaccc ccgttccgag atcctggccg cgccgccgta cgctcgggat cactcgccgg
     1801 agaccgtggc cgaggtgatg accgcgcgcg tgatctcgct tccggaggat gccgacgtgg
     1861 ccgaggccgc ccgccggatg ctggagaacc gcgtcaacca gatcccgatc acgcgggacg
     1921 accaggtggt cggcatcgtc gcccgccggg accttctgaa ggtggtcgct cgggaggacg
     1981 aggcgatccg tggcgagttg ctcgacctgc tgcgggaggg cgacggcctc ctcgggccgt
     2041 acgacgtcga tgtcgtggag ggcgtggcgc tgctgcgcgg ggggcgggac cggtcctcgc
     2101 gacgcctggc ggacatcctg gcccggagcg tccccggcgt gatcgaggtc cggttcgagg
     2161 acgccggaac cggaagcgag tgacggcacc gtccacacgc gaggcctccg ccggcctgtc
     2221 cccggcggag gcgcgggcgc gcctcgagcg cgtcggcccg aacgagctcg ccgccgcgcc
     2281 gcgccggccc tggccgctgc gtct
//
LOCUS       DASPWO010000163         3452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1125502, whole genome shotgun sequence.
ACCESSION   DASPWO010000163 DASPWO010000000
VERSION     DASPWO010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125502"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VEO00_03685"
     CDS             complement(<1..351)
                     /locus_tag="VEO00_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYU57136.1"
                     /translation="MIETTVDGRYQVIARVASGGMGEVYRAHDAVLDREVALKVLHSN
                     LASDDGFIDRFRREARAAAMLNHPNIVAVHDWGATDGTYFMVMEFIRGHSLRQILSRQ
                     GRLAPGQAAEVVLQV"
     gene            419..1225
                     /locus_tag="VEO00_03690"
     CDS             419..1225
                     /locus_tag="VEO00_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57137.1"
                     /translation="MRESVEAPREPVERTPPSSWVEPAADEGTLKRAFVDVERAVEVG
                     NADLRALGFWELVGRAKRDPMRAGVLAEMIGRIDRREFEARARPRFPVWLGTLALSAG
                     LLASAALVVLSAWLVYRDPPLPTLAGVLLVAAAVGLSVTAHDLGHWLGGRLVGIRFLG
                     YFLDGPFRIQPGLKTDYASYLRATAGQRAWMHASGALASKLAPFAVFAAAYVPHALND
                     WSLYPPWSLWAVLVVGAIQVLTDVLWSRKRSDWKKVARECRAARDLAARQ"
     gene            1237..2409
                     /locus_tag="VEO00_03695"
     CDS             1237..2409
                     /locus_tag="VEO00_03695"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HYU57138.1"
                     /translation="MEQLNPMLRNWASELDPLTRAQAEMLSRLPILAGPVALMPDAHL
                     GKGATVGAVIPTEGAIMPSAVGVDIGCGMIAVETDRTLAQLPDDLEPLLRGFAAGIPA
                     GLGRWHEHAGDAARAWLAAHPHVGLTPEQEARALVQFGTLGSGNHFVEVAADERGVVW
                     LVMHSGSRGVGNQLATKHMALARRQEQGLEDPDLGYFEQGQPRFEAYVRDMLWAQDYA
                     SANRRALMAAAIAELKRRTAGRDVRWINCHHNYAAREVHGGRELWITRKGAIRAGVGD
                     WGVVPGSMGAKSFIVRGLGSPLSYESSAHGAGRRMSRGQARREVSVEAFVRAMAGRTW
                     QAADAERLVDESPQAYKDIDQVMRDQRDLVQIVHELHGVVSYKGVERERRRRRARR"
     regulatory      2447..2565
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2562..3377
                     /locus_tag="VEO00_03700"
     CDS             2562..3377
                     /locus_tag="VEO00_03700"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HYU57139.1"
                     /translation="MADPLRIGDRTFSSRLILGTGKFGSLEAMRDAIGASGTEIVTVA
                     LRRVDLESSGGPDILEFIDRERVLLLPNTSGAMDAEEAVRLARLARAAGMPAWVKLEI
                     TPEPRYLLPDAVETLRAAEALVADGFTVLPYINADPLLAKRLEEAGCATVMPLGSWIG
                     SNRGVRTRDAIRIIVEQATVPVVVDAGLGAPSHAAEAMELGCDAVLVNTAIAVARDPV
                     AMAHAFRLGTEAGRRAFLAGRAAPREAAEPSSPLTGFLDKLVTSQADAAVTEP"
BASE COUNT          454 a         1194 c         1329 g          475 t
ORIGIN      
        1 cacctggagc accacctcgg ccgcctggcc gggagcgagg cggccctgcc gcgagaggat
       61 ctggcgcagg ctgtggccgc ggatgaactc catgaccatg aagtacgtgc cgtcggtcgc
      121 gccccagtcg tggacggcca cgatgttcgg gtgattgagc attgcggccg cgcgggcctc
      181 gcgccggaac cggtcgatga acccgtcgtc cgacgccagg ttggagtgca ggaccttcag
      241 cgccacctcg cggtcgagca cggcgtcgtg ggcacggtag acctcgccca tgccgccgga
      301 tgcgacgcgg gcgatgacct ggtagcggcc gtcgacggtg gtctcgatca cgtgctcatc
      361 ctggggggag gcggcccgag ccatcattgt agggttggcc tggtcgggag gcgggaacgt
      421 gcgcgaatcg gtggaggcac cgagggagcc ggtcgagcgc accccgccgt cgtcctgggt
      481 cgaaccggcg gccgacgagg ggacgctgaa gcgcgcgttc gtcgacgtcg agcgcgcggt
      541 cgaggtcggg aacgccgacc tgcgggcgct cggcttctgg gagctcgtcg gccgggccaa
      601 gcgcgacccg atgcgcgccg gggtgctggc ggagatgatc ggccggatcg accgaaggga
      661 gttcgaggcg cgggcgcgtc ctcggttccc ggtctggctc ggcaccctcg cgctctccgc
      721 cgggctgctg gcgagcgcgg cgttggtggt gctctcggcc tggctggtct accgcgaccc
      781 tccgctcccg acgctcgccg gagtgctgct cgtggccgcg gcggtcgggc tgtcggtgac
      841 cgcgcacgac ctcggccact ggctgggcgg ccgcttggtc gggatccgct tcctcggcta
      901 cttcctcgac gggccgtttc gcatccagcc gggtctgaag accgactacg cgtcgtacct
      961 gcgcgccacg gcggggcagc gggcgtggat gcacgcgtcc ggggcgctgg cctcgaagct
     1021 cgcgccgttc gcagtgttcg cggccgccta cgttccacac gcgctgaacg actggtcgct
     1081 ctacccaccg tggtcgctgt gggcagtgct cgttgtgggc gcgatccagg tcctcaccga
     1141 cgtcctctgg tcgcggaaga ggtccgactg gaagaaggtc gcccgcgagt gccgcgccgc
     1201 ccgcgacctc gcggcccggc agtaggcggc gggtccgtgg agcagctcaa ccccatgctg
     1261 cggaactggg cgtccgagct cgacccgctg acgcgcgcgc aggcggagat gctcagccgg
     1321 ttgccgatcc tcgccgggcc cgtggcgctg atgccggacg cgcacctcgg caagggcgcg
     1381 acggtcggcg cggtgatccc gaccgagggc gcgatcatgc cctccgcggt cggcgtggac
     1441 atcggctgcg ggatgatcgc ggtcgagacc gatcgcaccc tcgcgcagct tcccgacgat
     1501 ctcgagccgc tgctgcgggg gttcgccgcg ggcatcccgg ccgggctcgg ccgctggcac
     1561 gagcacgccg gcgacgccgc ccgggcctgg ctcgcggccc acccgcacgt cggcctgacg
     1621 ccggaacagg aggcgagggc gctcgtccag ttcgggacgc tcggctccgg caaccacttc
     1681 gtcgaggtcg ccgccgacga gcgcggcgtg gtctggctcg tgatgcactc cggctcgcgc
     1741 ggggtcggga accagctggc gacgaagcac atggcgctcg cccggaggca ggagcagggg
     1801 ctggaggacc cggacctcgg gtacttcgag caggggcagc cgcggttcga agcctacgtg
     1861 cgggacatgc tgtgggcgca ggactacgcg tccgcgaacc gccgcgccct gatggcggcg
     1921 gcgatcgccg agctcaagcg gcggactgcc gggcgcgacg tgcgctggat caactgccac
     1981 cacaactacg cggcccgcga ggtgcacggt gggcgcgagc tgtggatcac gcgcaagggc
     2041 gccatccgcg ccggcgtcgg ggactggggc gtggtccccg ggagcatggg cgcgaagtcg
     2101 ttcatcgtgc gtggcctggg aagcccgctc tcctacgaga gcagcgcgca cggcgcgggg
     2161 cggcggatga gccgcgggca ggcccggcgt gaggtgtcgg tggaggcgtt cgtgcgggcg
     2221 atggccggcc ggacgtggca ggcggccgat gccgagcgcc tggtggacga gagcccgcag
     2281 gcctacaagg acatcgacca ggtgatgcgc gaccagcgcg acctggtgca gatcgtccac
     2341 gagctgcacg gcgtggtgag ctacaagggc gtggagcggg agcgccgccg ccgccgggca
     2401 cgccgctagc cggggccgga ccttcgggag ccggacctat acttggatcg acccaccggg
     2461 gagctcgggg caaccgggct gagaggccgg ctcgaggtga ggcgccggcg acccgcagaa
     2521 cctgatccgg gtaacgccgg cgaagggagg cgaccatcac catcgcagac ccactccgca
     2581 tcggggaccg cacgttctcg tcgcgcctga tcctcgggac cggcaagttc ggctccctcg
     2641 aggccatgcg cgacgccatc ggggcgtcgg ggaccgagat cgtgaccgtg gcgctgcgcc
     2701 gggtggacct cgagtcgagc ggcgggccgg acatcctcga gttcatcgac cgcgagcggg
     2761 tcctgctcct gccgaacacc tcgggggcca tggacgccga ggaggcggtg cggctggcgc
     2821 gcctggcccg tgcagccggc atgcccgctt gggtgaagct cgagatcaca cccgagccgc
     2881 gctacctgct gcccgacgcc gtcgagacgc tccgcgccgc ggaggcgctc gtcgccgatg
     2941 gcttcaccgt gctgccctac atcaacgccg acccgctgct ggcgaagcgc ctcgaggagg
     3001 ccggctgcgc gaccgtcatg ccgctcggct cgtggatcgg ttcgaaccgg ggcgtgcgga
     3061 cgcgcgacgc gatccggatc atcgtcgaac aggccacggt tccggtggtc gtcgacgccg
     3121 gcctcggcgc gccgtcgcac gccgccgagg cgatggagct cggctgcgat gccgtcctgg
     3181 tgaacaccgc catcgcggtg gcccgcgatc cggtggcgat ggcccacgca ttccggctgg
     3241 ggaccgaggc cgggcggcgg gcgtttctcg ccgggcgggc cgcgccgcgc gaggcggccg
     3301 agccgtccag tccgctgacc gggttcctcg acaagctcgt cacgtctcag gccgatgcgg
     3361 cggtgaccga gccgtgaccg accaccgcgg ggacgggttc ctcgagcctc ggccagagcg
     3421 gacgttcgcc gacgatctgg cgacgctgcc ct
//
LOCUS       DASPWO010000164         3352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1125521, whole genome shotgun sequence.
ACCESSION   DASPWO010000164 DASPWO010000000
VERSION     DASPWO010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125521"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VEO00_03705"
     CDS             complement(<1..245)
                     /locus_tag="VEO00_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYU57140.1"
                     /translation="MAATRSAGTEAVLGALSRRPGRTAAEIAEIAGIGRSTANKALAA
                     LEAEGKAVRAPGVRDGARRRPDRWSLPPKSKSPSSGT"
     gene            400..1287
                     /locus_tag="VEO00_03710"
     CDS             400..1287
                     /locus_tag="VEO00_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HYU57141.1"
                     /translation="MAEAPSRSKNSRTGAVPLAVWPVAQVSAQYQRAGRYHPDSSAHP
                     GKMLPALARRIVEEYSEPGALVVDPMAGSGTTLVEAAALGRRAVGVELESRWVALARQ
                     NLDCALSPEHRRLAQIRRGDARRLPTLLGHLAGTVDLVVVSPPYACDAGVIDKPAWLS
                     GRTLCDFDSLNYSRDKSNVGHARGDAYVASMAEIYAGCHKVLRAGGLLVTVTKNTRRA
                     GRAFDLAGTTVALSRSVGFVYLQHVVALLAAIRDGSLFARPSFWQLTQIRRARGRGEP
                     AHLVVHEDVCVFAKPEERR"
     gene            1284..2129
                     /locus_tag="VEO00_03715"
     CDS             1284..2129
                     /locus_tag="VEO00_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018541921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HYU57142.1"
                     /translation="MSADLPMSVWTTAQTHVRQQRAGRYVPESARHPGRMLPAIARAA
                     ITAYTAPGDLVLDPMCGVGTTLVEAMHSGRDALGVEYEPEWADITRANLDHAVSQGAS
                     GRGEVITGDARHLSSLVDPHVRGTVAMVLTSPPYGASVHGQATPVPGRGVLKSHDRYS
                     KDRANLAHQPLGRLLCAFTSILVSCRDLLRPGGVVAVTARPFRRSGELVDLPTAVLRC
                     GDAAGLVPYERNVALLAGLREDRLVPRASFFALDQVRRARRAGRPLQVIAHEDVLVFR
                     RALRA"
     gene            2350..>3352
                     /locus_tag="VEO00_03720"
     CDS             2350..>3352
                     /locus_tag="VEO00_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HYU57143.1"
                     /translation="MTTVPNGAAPRYAWYGRVSTEDEQDPTLSFPRQLGNAEARVAEG
                     GGRIVAHFYDVESGTGTLAARGKGGLAGFDIPIPRDGGLQELLAAAARRPRGFDRVIV
                     ESISRLSRNSSVAFRVEDDLRTAGVRLCCADEPLEESFGTIVLRHVNIGIARGYHHEL
                     MVKSRQGLETSTRQGWHTGGIAAYGYRFVEHDHPNPHKAARGQRKRTLEPDPVRAPVV
                     RRIYDMYLSGGVGITQIRDALNAHPGRYPPPTCVDPARALGAWSRSSVWEILRNPKYT
                     GYQVWNRRARKTAGNRDNPPEAWIWSEEPAHPAVVSKEEFEAVAARAAANRGSRQGAA
                     "
BASE COUNT          470 a         1197 c         1152 g          533 t
ORIGIN      
        1 ttggtcccgg agctcgggct cttgctcttt gggggcagcg accaacgatc gggccggcgg
       61 cgggccccgt cccggacgcc cggcgcccgg acggccttgc cctcggcctc cagtgccgct
      121 agggccttgt tggccgtgga ccggccgatc ccagcgatct cggcgatctc ggccgcggtg
      181 cggccgggtc gccttgacag ggcgccgagc accgcctcgg tgccggcgct tcgtgtggct
      241 gccatcgcga tctccttccc ctggtggttg tccgggaccg gaggggttac ctcgacccgg
      301 ccggagatct caagcccgct acccagccgg gatttgtgac ttgcgggggg gctcggatgc
      361 ggttcatgag gtggcgcaac cgcggccgtg ggccaaccca tggccgaagc tccgtcccgt
      421 tccaagaact cccgtacggg cgccgtcccg ctcgcggtgt ggccggtggc gcaagtctcg
      481 gcccagtacc agcgagccgg gcgctaccac cccgacagct ccgcccatcc cggcaagatg
      541 cttcccgcgc tggcccggcg catcgtcgag gagtattcag agcccggagc tctcgtcgtc
      601 gatcccatgg ccgggagcgg tactactctg gtcgaggcgg ctgcgcttgg tcggcgcgcg
      661 gtcggtgtgg agctggaatc gcggtgggtg gcgctcgccc gccagaacct cgactgcgcc
      721 ttatctccgg agcaccgacg gctggctcag atcaggcgag gagacgcccg ccgccttccg
      781 acgctgctcg gccatctcgc ggggacggtc gacctcgtgg tggtgtcgcc gccctacgcc
      841 tgcgacgcag gcgtcatcga caagcccgca tggctctcgg gacgaaccct ctgcgacttc
      901 gacagcctca actactcccg ggataagtcc aacgttggtc acgctcgcgg cgacgcttac
      961 gtcgcatcga tggccgagat ctacgcgggc tgccacaagg tgcttcgagc cggcggcctg
     1021 ctggtgacgg ttacgaagaa cacccgccgg gccgggcgcg cgttcgatct cgccggcacg
     1081 acggttgcgt tgtcgagatc cgtcgggttc gtctacctcc aacacgttgt cgccctgctg
     1141 gccgcgatcc gtgacgggag tctgttcgcc cggccctcgt tctggcagct cacccagatc
     1201 cgtagggccc gcgggcgggg cgagcccgcg cacctcgtcg tgcacgagga cgtttgcgtc
     1261 ttcgccaagc cggaggagcg tcgatgagtg cggacctccc gatgtccgtg tggacgacgg
     1321 cccagacgca cgtccgccag cagcgcgccg gccggtacgt tccggagtcc gcgaggcacc
     1381 cgggaaggat gctgccggcg atcgcccgcg ccgcgatcac cgcgtacacc gctccgggtg
     1441 acctcgtcct cgatcccatg tgcggcgtgg ggacgaccct cgtggaggcg atgcattccg
     1501 gtcgcgacgc gctcggcgtg gagtacgagc ccgagtgggc cgatatcacc cgggcgaatc
     1561 tcgatcatgc cgtgtcacag ggcgcgagtg ggcgaggcga ggtcatcacc ggagatgccc
     1621 ggcatctgtc gagtctcgtc gacccccacg tccgcgggac ggtcgccatg gtgctgacct
     1681 ctccgcccta cggggcgtcg gtgcatgggc aagccacacc ggttccgggc cgtggcgtcc
     1741 tgaagtcgca cgaccgatac tcgaaggatc gagccaacct ggctcaccag cccctcggcc
     1801 gcttgctctg cgcgttcacc tcgatcctcg tgtcgtgcag agacctgctc cgacccggcg
     1861 gcgtcgtggc cgtgaccgcg cggccgttcc gacgctccgg agaactggtg gacctcccga
     1921 cggccgttct tcggtgcggt gacgctgcag gcctcgtgcc ctacgaacgc aacgtcgcgc
     1981 tcctggccgg gcttcgtgag gaccgtcttg ttcccagggc gtcgttcttc gccctcgacc
     2041 aggtccgccg cgcgcgccgc gcagggcggc cgctgcaggt gatcgcccac gaggacgtac
     2101 tcgtgttccg gcgtgcgttg cgcgcatgac gcaaggccgg ccaacctcgg gcgagtccga
     2161 cggctcctcc gctcggagcc cgacttcgaa gacgagcaga tcccgccctc tgcacgtccg
     2221 gttccacgtc cgctacgtgg gaggggagga gggtcgagtc ctcgccgtgg cgcaaggacg
     2281 agctctccgg gccgcccttg ctgttctcgc ggagatggag gtaggacagg ctcgggagga
     2341 atgactgcga tgactacggt gccgaatgga gcggccccgc gctacgcgtg gtacggccgc
     2401 gtctccaccg aggacgagca ggacccgacg ctctccttcc cccggcagct tggcaacgcc
     2461 gaggccaggg tcgcggaggg cggcgggcgg atcgtcgctc acttctacga tgtcgagtcc
     2521 gggacgggaa cgctggcggc gcgaggcaag ggcggcctgg ctggcttcga cattcccatt
     2581 cctcgcgacg gcggtctcca ggagttgctg gcagccgcag cccggcgacc gcgcgggttc
     2641 gatcgcgtga tcgtggagtc gatctcacga ctctcacgca actcctccgt ggcgttccgg
     2701 gtcgaggacg atctccgcac cgccggggtc cggctgtgct gcgccgacga gccgctcgag
     2761 gagtcgttcg gcacgatcgt gctccgccac gtcaacatcg ggatcgcccg cggctaccac
     2821 cacgagctga tggtgaagtc gcgtcaggga ctggagacct ccacccgcca gggatggcac
     2881 accggcggca ttgctgccta cggctaccgc ttcgtcgagc acgatcaccc gaacccccac
     2941 aaagccgcac gtggtcagcg caagcgcacc cttgagcccg accccgtgcg cgccccggtg
     3001 gtccgccgca tctacgacat gtacctgtct ggtggggtcg gcatcaccca gatccgcgac
     3061 gcgctgaacg cccaccccgg ccgctacccg ccgcccacct gcgtcgaccc cgcgcgagcc
     3121 ttaggggcgt ggagccgctc cagcgtgtgg gagatcctgc ggaacccgaa gtacaccggc
     3181 taccaggtgt ggaaccgccg ggcgaggaag accgccggga accgagacaa cccaccggag
     3241 gcctggatct ggtcggagga gcccgctcac cccgcggtcg tctcgaagga ggagttcgag
     3301 gccgtcgccg cccgcgccgc cgcgaaccgt ggttcacgtc agggcgccgc ac
//
LOCUS       DASPWO010000165         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1209852, whole genome shotgun sequence.
ACCESSION   DASPWO010000165 DASPWO010000000
VERSION     DASPWO010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209852"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VEO00_03725"
     CDS             <1..306
                     /locus_tag="VEO00_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU57144.1"
                     /translation="RAIAAYLNVPVYAAFHEWLGRGDVLAPMWEAWKAGDREAATAAI
                     PDSLVDELIVHGSAADCRAHLARYAANGVTTPAPAILAGPDDARRAMRDLAPTATKA"
     gene            374..985
                     /locus_tag="VEO00_03730"
     CDS             374..985
                     /locus_tag="VEO00_03730"
                     /inference="COORDINATES: protein motif:HMM:NF019961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HYU57145.1"
                     /translation="MAIATQGTKALIVEVALRRFADHGYGGTSLNDIADEVGIRRQSL
                     LHHFPSKDALYRAVVLESFADWLALVEGATQEQVRQGWPQVERMLRAAFTFFEEHPEF
                     VRLARWEALVGGPVLTEELGALLRPLFERGVGFLEREMDAGRLRRYEPRQLLLTGYGA
                     ILSYLSDAPLISSLVDDDPLSPAALKERREHVIDVLRNALEPR"
     gene            complement(1021..1752)
                     /locus_tag="VEO00_03735"
     CDS             complement(1021..1752)
                     /locus_tag="VEO00_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HYU57146.1"
                     /translation="MTHDDYIAALRGNATAAADAARAAGMEARVPSCPEWTVRDLAAH
                     LGGHHRWVTGNLGRAPEDGMHPIGAYEPAPEGPAAADWIEEGAAALADRLAEAGPDQR
                     CWTWVPERSTSGFWARRTANETAVHRWDLQNAAGAPESFDADHAADAIDEYFGVMQVI
                     GSERLRGDSETIHWHCTDTDGEWLVRLAPDGLEVSREHAKADVAMRGPASDLLLVTIG
                     RAPASTIEVLGDAALVERWQAGAKF"
     gene            complement(1749..>2450)
                     /locus_tag="VEO00_03740"
     CDS             complement(1749..>2450)
                     /locus_tag="VEO00_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HYU57147.1"
                     /translation="LAERFGVDEVRLEEHEPHYNVAPRAEVPAVRRREDRKILSGLRW
                     GLVPSWAKDPKVGDRQINARAESVADKPAYRKAFEKRRCLIPADGFYEWKRQGEKRKQ
                     PMFVHLRSGEPMAFAGLWEIWKDPKVARSKDGGEDEGWLRTCAIVTTDANDVLTPIHD
                     RMPVILPEPAWDAWLDPDADPERLRRFLVPAPDDLIAVYPVSPMVNSADNDGPELVQP
                     LAPDPQLPNLEGEVQ"
BASE COUNT          318 a          881 c          875 g          376 t
ORIGIN      
        1 cgggcgatcg cggcgtacct caatgtgccc gtgtacgccg cgttccacga atggctgggt
       61 cgcggtgacg tgctcgcgcc gatgtgggag gcatggaagg cgggtgaccg cgaggccgcg
      121 accgcggcca tccccgactc gctcgtggac gagctcatcg tgcacggatc cgcggccgat
      181 tgccgcgccc acctggcgcg ctacgccgcc aacggagtca cgacgcccgc gcccgcgatc
      241 ctggccgggc ccgacgatgc ccgccgggcg atgcgggatc tcgcgcccac ggcgacgaag
      301 gcgtagcctc atgtcgacgg gccgcgttcc ctctccctcc gttggaggca gcgccttccc
      361 tccggaagag acgatggcca tcgctacgca gggtacgaag gccctgatcg tcgaggtcgc
      421 gctccggcgc ttcgccgatc acggatacgg cggcaccagc ctcaacgaca tcgccgacga
      481 ggtcgggatc cgccgccaga gcctgctcca tcacttcccg tcgaaggatg cgctctaccg
      541 ggcggtggtg ctggagtcgt tcgccgactg gctcgcgctg gtggagggcg cgacccagga
      601 gcaggtccgt cagggctggc cccaggtcga gcggatgctg cgggcggcct tcaccttctt
      661 cgaggagcac cccgagttcg tgcgcctggc ccgctgggag gcgctggtcg ggggcccggt
      721 cctgaccgag gagctcggtg cgctgctgcg gcccctcttc gagcgaggcg tgggcttcct
      781 cgagcgggag atggacgcgg gtcgcctacg ccgctacgag ccgcgccagc tgctgctgac
      841 cggctatggc gcgatcctct cctatctctc cgacgcgccc ctcatcagct cgctggtcga
      901 cgacgacccg ctgagcccag ccgccctcaa ggaacggcgg gagcacgtca tcgacgtgct
      961 gcggaacgcg ttggagccgc gttaggagct gccatcgccg acgagggggc cgggagagag
     1021 ctagaacttc gcgcccgctt gccagcgctc gacgagcgcg gcgtcaccga ggacctcgat
     1081 ggtcgaggcg ggcgcgcgac cgatcgtcac gagcaggagg tcggaggccg ggccgcgcat
     1141 cgcgacgtcc gccttggcgt gctcgcgact gacttccaac ccgtcgggcg cgagccggac
     1201 gagccactcg ccgtcggtgt cggtgcaatg ccagtggatg gtctcgctgt cgccccgcag
     1261 ccgctcgctg ccgatcacct gcatgacccc gaagtactcg tcgatggcgt cggcggcatg
     1321 gtcggcgtcg aacgattcgg gggcgccggc ggcgttctgc aggtcccagc ggtggaccgc
     1381 ggtctcgttg gcggtgcggc gagcccagaa cccgctcgtc gagcgctctg ggacccaggt
     1441 ccagcagcgc tggtcagggc cggcctcggc gagccggtcg gcgagggcgg cggcaccctc
     1501 ctcgatccag tcagccgccg ccggcccctc gggcgcgggc tcgtaggcgc cgatggggtg
     1561 catgccgtcc tccggggcgc ggccgaggtt gccggtcacc caccggtggt gcccgccgag
     1621 gtgcgccgcc aggtcgcgca cggtccactc cggacacgac ggcacccggg cttccatccc
     1681 ggccgcgcgc gccgcatcgg cggcggcggt cgcgttccct ctcagggcgg cgatgtagtc
     1741 gtcgtgggtc actgcacctc cccttcgaga ttaggcagtt gcgggtcggg cgcgagcggc
     1801 tgcacgagct cgggaccgtc gttgtcggcg ctgttcacca tcgggctcac gggatagacg
     1861 gcgatgaggt cgtcgggggc cggcaccagg aagcgccgaa gcctctcagg atcggcgtcg
     1921 ggatccagcc aggcgtccca ggccggctcc gggaggatca ccggcatgcg gtcgtggatc
     1981 ggcgtcagca cgtcgttcgc gtccgtggtc acgatcgcgc atgtccgcag ccagccctcg
     2041 tcctcgccgc cgtctttgga ccgggcgacc ttcgggtcct tccagatctc ccacagcccc
     2101 gcgaaggcca tgggctcccc ggaacgcagg tgcacgaaca tcggttgctt gcgcttctcc
     2161 ccctgccttt tccactcgta gaagccgtcg gcggggatca ggcagcggcg cttctcgaac
     2221 gccttccggt aggcgggctt gtcggctacc gactcggccc gggcgttgat ctggcggtcc
     2281 cccaccttcg ggtccttcgc ccacgacggc accaggcccc agcgcaggcc ggacaggatc
     2341 ttccgatcct cgcggcggcg gacggccggg acctcggccc ggggcgccac gttgtagtga
     2401 ggctcgtgct cctcgagccg gacctcatcc acgccgaacc gctcggccag
//
LOCUS       DASPWO010000166         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_280957, whole genome shotgun sequence.
ACCESSION   DASPWO010000166 DASPWO010000000
VERSION     DASPWO010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280957"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="VEO00_03745"
     CDS             complement(<1..1481)
                     /locus_tag="VEO00_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018729103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HYU57148.1"
                     /translation="MLDVNFFDEIRIGLATADQIRAWSNGEVKKPETINYRTLKPEKD
                     GLFCERIFGPTRDWECHCGKYKRVRFKGIICERCGVEITRSKVRRDRMGHIELAAPVT
                     HIWYFKGVPSRLGYLLDIAPKDLERIIYFAAYVITRIDTDRRHKELPEIEKEIEQEQN
                     ELEAERDDEIKTRLSELEERLRELEGEGAKGAQLSAARREAQRDVDRITETTKRETEH
                     LDEVLTGFKNLSVKQLVADDAVYRSLRERFGDYFDGGMGAEAIKRLLQDLKLEEEAES
                     LREQIATGKGTRRLKAIKRLKVVSNFTEGRNSPMGMVLDAVPVIPPDLRPMVQLDGGR
                     FATSDLNDLYRRVINRNNRLKRLLDLQAPEIIVNNEKRMLQEAVDALFDNGRRGRPVT
                     GPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELRLHQCGLPKQMALE
                     LFKPFVMKRLVDQALAQNIKSAKRMVERARPQVWDVLEEVIKE"
     gene            complement(1544..>2441)
                     /locus_tag="VEO00_03750"
     CDS             complement(1544..>2441)
                     /locus_tag="VEO00_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HYU57149.1"
                     /translation="GVNQLVRVYVAQKRKISDGDKLAGRHGNKGVISTILPEEDMPFL
                     ADGSPVDIILNPLGVPSRMNLGQVLEAHLGWAGLVGFRKDGQDHEGPVYVSTPVFDGA
                     REHEILEAIRSAHPNKDGVRLVDDVGKAVLYDGRNGEPYEEPVTVGVAYILKLLHLVD
                     DKIHARSTGPYSMITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYALQELLTIKSDDV
                     LGRVKVYEAIVKGENIPEPGIPESFKVLIKEMQSLCLNVEVRSAEGEEIELKETDEDV
                     FRAAEELGIDLSRREPAGADYD"
BASE COUNT          343 a          850 c          785 g          463 t
ORIGIN      
        1 tgctccttga tgacctcctc gagcacgtcc cagacctgcg ggcgggcccg ctccaccatg
       61 cgcttggcgc tcttgatgtt ctgcgcgagc gcctggtcga cgagccgctt catgacgaag
      121 ggcttgaaca gctcgagcgc catctgcttg ggcaggccgc actggtgcag gcggagctcc
      181 ggaccgacca cgatcaccga acggcccgag tagtccacgc gcttgccgag caggttctgg
      241 cggaaccgcc cctgcttgcc cttcaacatg tcggacagcg acttcagcgg tcggttgccg
      301 ggacccgtga cgggtcggcc gcggcggccg ttgtcgaaca gggcgtcgac ggcctcctgc
      361 agcatccgct tctcgttgtt gacgatgatc tcgggcgcct gcaggtcgag gagtcgcttc
      421 aggcggttgt tccggttgat cacgcggcgg tacaggtcgt tcaggtcgct ggtcgcgaac
      481 cggccaccgt cgagctgcac catgggtcgc aggtccggcg ggatcaccgg cacggcgtcg
      541 agcaccatgc ccatgggcga gttccgaccc tcggtgaagt tggagaccac cttcaggcgc
      601 ttgatggcct tgaggcgccg cgtgcccttg cccgtggcga tctgctcgcg gagcgactcg
      661 gcctcctcct ctagcttgag gtcctggagc aggcgcttga tcgcctccgc ccccatgccg
      721 ccgtcgaagt agtcgccgaa ccgctcgcgg agcgatcggt agacggcgtc gtcggcaacc
      781 agttgcttga ccgacaggtt cttgaacccg gtcaggacct cgtccagatg ctcggtctcg
      841 cgcttggtcg tctcggtgat ccggtcgacg tcgcgctggg cttcgcgtcg ggcggccgag
      901 agctgggcgc ccttggcccc ctcgccctcg agctcccgca ggcgctcctc gagctcggac
      961 aggcgggtct tgatctcgtc gtcgcgctcg gcctcgagct cgttctgctc ctgctcgatc
     1021 tccttctcga tctcgggcag ctccttgtgg cgccggtccg tgtcgatccg cgtgatgacg
     1081 taggcggcga agtagatgat ccgctcgagg tccttgggcg cgatgtccag gaggtagccg
     1141 agccggctcg ggacgccctt gaaataccag atgtgcgtga ccggggcggc cagctcgatg
     1201 tgccccatcc ggtcgcggcg gaccttgctg cgggtgatct cgaccccgca gcgctcgcag
     1261 atgatgccct tgaagcggac gcgcttgtac ttgccgcagt ggcactccca gtcccgcgtc
     1321 ggaccgaaga tccgctcgca gaacagaccg tccttctccg gcttcagcgt ccggtagttg
     1381 atcgtctccg gcttcttgac ctcgccgttg gaccacgcgc ggatctgatc cgcggtggca
     1441 aggccgatcc ggatctcatc gaagaaattc acatccagca cgtaggcggc ctcctcacga
     1501 cctcaccctg caccatgttc aacaacagct tgttcccgcc ccgctagtcg tagtcggcgc
     1561 cggccggctc cctgcgggac aggtcgatcc cgagctcctc ggccgcgcgg aacacgtcct
     1621 catcggtctc cttgagctcg atctcctcgc cctcggccga acgcacctcc acgttcaggc
     1681 agagggactg catctccttg atcaggacct tgaaagactc ggggatcccc ggctcgggga
     1741 tgttctcgcc cttgacgatg gcctcgtaga ccttgaccct gccgaggacg tcgtcggact
     1801 tgatcgtcag cagctcctgg agcgcgtagg ccgcgccgta tgcctcgagc gcccacacct
     1861 ccatctcgcc gaaccgctgc ccgccgaact gcgccttccc gccgagcggc tgctgcgtga
     1921 tcatcgagta gggaccggtg gaccgggcgt ggatcttgtc gtccaccagg tggagcagct
     1981 tcaggatgta ggccaccccg accgtgacgg gctcctcgta cggctcgccg ttgcgaccgt
     2041 catacaggac cgccttgccg acgtcgtcga ccaggcggac cccgtccttg ttgggatgcg
     2101 cgctgcggat cgcctcgagg atctcgtgct cgcgggcgcc gtcgaacacc ggcgtcgaca
     2161 cgtacacggg gccctcgtgg tcctgcccgt ccttgcggaa cccgaccagg ccggcccacc
     2221 cgaggtgcgc ctcgagcacc tgcccgaggt tcatccggga cgggacgccg agcgggttca
     2281 ggatgatgtc aacggggctt ccgtcggcga ggaacggcat gtcctcctcc ggcaggatcg
     2341 tcgagatcac gcccttgttc ccgtggcggc cggcgagctt gtcgccgtcg gagatcttgc
     2401 gcttctgcgc cacgtagacg cggaccagct ggttcacgcc g
//
LOCUS       DASPWO010000167         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_617162, whole genome shotgun sequence.
ACCESSION   DASPWO010000167 DASPWO010000000
VERSION     DASPWO010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617162"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="VEO00_03755"
     CDS             <1..1535
                     /locus_tag="VEO00_03755"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HYU57150.1"
                     /translation="SPTTSPTETIPEQGAELTSEQIRAGQIFLRGVQVQPSRQSSTLL
                     NVRVANADPRKATKKAVALGLAYLGFKHSEALAAGAQAKANLQAQIADKQAELAEAEA
                     TAEQDTTGRASGGVAQLEQDLGALQFQLQQLKQAITEATGAGDIINPTLPSRKPVRPN
                     HIRDALFAIIVGMVLGLGAAFLRDYLDDSLKGTEDVERQSGAALIGVVPHVQAAGRQI
                     EARNGDRERSYLVTLDDPKAPSSEAYRTLRTNLLFMSVSGPVGRLLITSPVAGEGKST
                     TAANLAVVLAQAGQRVLLVGSDLRRPSVHRFFGVSNRVGLSSVLVGQASLMEAVADPG
                     IKGLRVMSGGPVPPNPTELLGSLAMKDFLDQAAQVTDWVLLDAPPVLGLADAAVLATL
                     SDAALLVVNEATSRRVLAHARDQLAKVEARVVGTILNNFGPSFSYYYSDYYAYTSQYY
                     LAPETQQNGKLSRKDRRRQRKAEETAGRGAGLAFEEEEKPQPVGQRSARSAEDFWNEN
                     PT"
     gene            1572..2702
                     /locus_tag="VEO00_03760"
     CDS             1572..2702
                     /locus_tag="VEO00_03760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU57151.1"
                     /translation="MTARPARVLWVIKGLGPGGAERLVADIVPRLDRDRFRAEVAYLL
                     PWKDHLVPELRAAGIPVRCLEVRRHADPRWVGRLRRLLEGGDGGGRVDLVHAHLPFAA
                     IGARVAARRVRGRRPAVVYTEHNLWDRYRGATARLNAATFSWNDRVIAVSAAVAASIR
                     ARSGGPPVTVIENGVDGERLRADALPRAEARSELGATDDDLVIGTLGGITAKKGHRHL
                     VRAARRVLERCPRALFLFVGLGADEEPVRREIAEQGVGERVRLLGYRERASRLLPGFD
                     LFCLSSLYEGLPVALLEALALGVPAVATRVGGVPDVLAAGGGVVVEPGDAGALAAGLI
                     ALAEDPDRRAALAARAPAVADRYGLERTVRAVEGVYDEVLHR"
     gene            2699..>3108
                     /locus_tag="VEO00_03765"
     CDS             2699..>3108
                     /locus_tag="VEO00_03765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU57152.1"
                     /translation="MNGELTVRPMGDGDLDAVVDLLTESLGPAPGGAGRRDLFVWKHL
                     QNPFGRSIVLLAEDGAGRPAGLRAFMRWSLAVPGGGTVDAVRAVDTATSPAFQRRGVF
                     TRLTTEGLETCAAAGVRLVFNTPNEKSLPGYLKMG"
BASE COUNT          409 a         1132 c         1157 g          410 t
ORIGIN      
        1 gttctccgac cacctcgccg accgagacca tcccggagca aggcgcggag ctcacgtccg
       61 agcagatccg cgcgggccag atcttcctcc gcggcgtcca ggtacagccc tcccgccagt
      121 ccagcacgct gctgaacgtg cgggtggcga acgccgaccc gcggaaggcg acgaagaagg
      181 cggtggcgct cgggctggcc tacctggggt tcaagcacag cgaggccctg gcagccgggg
      241 cgcaggccaa ggcaaacctt caggcacaga tcgcagacaa gcaggccgag ctcgcggagg
      301 cagaggctac ggcggagcag gacacgacgg gccgggcgag cggtggggtt gcgcagctcg
      361 agcaggatct cggcgcgctg cagttccagt tgcagcagct taagcaagcg atcaccgagg
      421 cgaccggggc cggagacatc atcaacccga ccctgccgtc gcggaagccg gtccggccga
      481 accacatccg cgacgctctg ttcgcgatca tcgtcgggat ggtgctgggg ctgggggcgg
      541 cgttcctgcg cgactacctc gacgacagcc tgaaggggac ggaggacgtc gagcggcagt
      601 cgggcgcggc gctgatcggc gtcgtcccgc acgtgcaggc cgccgggagg cagatcgagg
      661 cacgcaacgg cgaccgcgag cgctcgtatc tggtcaccct cgacgacccg aaggcaccct
      721 cctccgaggc ctaccgcacg ctgcgcacca acctgttgtt catgtcggtt tccggcccgg
      781 tcggacgcct gctgatcacc tcgccggtgg ccggcgaggg caagtcgacc accgccgcga
      841 acctggccgt cgtcctggcc caggcgggcc agcgcgtgct gctggtcggc tccgacctgc
      901 gccggccgtc cgtgcaccgc ttcttcggcg tctcgaaccg cgtcgggctg tcgagcgtgc
      961 tcgtcggcca ggcctcgctg atggaggcgg tcgccgaccc ggggatcaag ggcctgcggg
     1021 tgatgtccgg cggcccggtc ccgccgaacc cgaccgagct gctcggctcg ctggcgatga
     1081 aggacttcct ggaccaggcc gcgcaggtca ccgactgggt gctgctcgac gcgccgccgg
     1141 tcctcgggct ggccgacgcc gccgtgctgg ccaccctgtc cgacgccgcg ctcctcgtcg
     1201 tgaacgaggc caccagccgc cgggtgctgg cccacgcccg cgatcagctg gcgaaggtcg
     1261 aggcgcgcgt ggtcgggacc atcctgaaca acttcgggcc gtcgttctcc tactactact
     1321 cggactacta cgcctacacc agccagtact acctggcccc ggagacgcag cagaacggca
     1381 agctctcccg gaaggaccgc cgtcgccagc gcaaggccga ggagacggcg ggccgcggcg
     1441 ccgggctcgc cttcgaggag gaggagaagc cccagcccgt cgggcagcgc tccgcgcgca
     1501 gcgccgagga cttctggaac gagaacccca cctaagcttc ccctcgtgac gacggggatg
     1561 cgggccgtcc cgtgacagcg cggccggccc gagtgctctg ggtgatcaaa gggctggggc
     1621 cgggcggcgc cgagcgcctg gtcgccgaca tcgtcccgcg cctcgaccgc gaccgcttcc
     1681 gggccgaggt cgcctatctg ctgccgtgga aggaccacct cgtccccgag ctccgcgcgg
     1741 ccggcatccc cgtgcgctgc ctggaggtcc gccgccatgc tgacccgcgc tgggtgggcc
     1801 gcctgcgccg cctgctcgag ggcggcgacg gcgggggacg ggtggacctg gtccacgccc
     1861 acctgccctt cgccgccatc ggcgcacggg tggccgcgcg gcgcgtcaga ggccggcggc
     1921 cggccgtcgt ctacaccgaa cacaacctgt gggaccgcta ccgcggcgcg accgcacggc
     1981 tgaacgcggc cacgttctcc tggaacgacc gggtcatcgc ggtctccgcc gcggtggcgg
     2041 cgtcgatccg cgcccgctcg ggcgggcccc ccgtgaccgt gatcgagaat ggcgtggacg
     2101 gcgagcgcct gcgggcggat gcgctgccgc gcgccgaggc ccgctcggag ctcggggcca
     2161 cggacgacga cctggtgatc ggcaccctcg gggggatcac cgccaagaag ggccaccgcc
     2221 acctggttcg cgcggcccgc cgggtcctgg agcgctgccc gcgggccttg ttcctgttcg
     2281 tcgggctggg cgccgacgag gaacccgtcc ggcgcgagat cgcggagcag ggggtcgggg
     2341 agcgggtccg tctgctcggc taccgcgagc gcgcctcccg gctcctgccg gggttcgacc
     2401 tgttctgcct gtcgtcgctc tacgaggggc ttccggtggc gctcctcgag gcgctcgccc
     2461 tgggggtccc ggcggtggcc acccgggtcg ggggcgtccc ggacgtgctc gccgccggcg
     2521 gcggggtggt ggtcgagccg ggcgatgccg gagccctggc cgccggtctg atcgccctgg
     2581 ccgaggaccc cgaccgccgg gcggcgctcg ccgcgcgggc gccggcggtc gccgaccgct
     2641 acgggctgga gcgcacggtc cgtgccgtcg agggcgtcta cgacgaagtg ttgcatcggt
     2701 gaacggcgag ttgacggtgc ggccgatggg cgacggcgac ctcgacgcgg tggtcgacct
     2761 gctgaccgag agcctcggcc ccgcgcccgg cggcgccggg cggcgggacc tgttcgtctg
     2821 gaagcacctc cagaacccgt tcggccgctc gatcgtcctg ctcgcagagg acggcgccgg
     2881 gcggcccgca gggctgcgcg cgttcatgcg ctggtcgctg gccgtcccgg gtggcgggac
     2941 ggtggacgcg gtgcgcgccg tggacaccgc gacgtccccg gccttccagc gccgcggcgt
     3001 gttcacgcgg ctgaccaccg aggggctcga gacctgcgcg gcggccgggg tgcgcctggt
     3061 cttcaacacc ccgaacgaga agagcctccc cggctacctg aagatggg
//
LOCUS       DASPWO010000168         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_956864, whole genome shotgun sequence.
ACCESSION   DASPWO010000168 DASPWO010000000
VERSION     DASPWO010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956864"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..957
                     /locus_tag="VEO00_03770"
     CDS             52..957
                     /locus_tag="VEO00_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HYU57153.1"
                     /translation="MDGLTPVQRRTLDDLMGATVPRPVFGEDLAGRLRAEVEGGVAAL
                     SFPDPPVWLGKHRLNDHGRCEGLFVAGLLGEGAAFEHRPQTAAGSLAHKAIELDVPRG
                     RADPTPALVERAAARLAEGDTGFAAFWEGLDPLEASEISQMAVRLAEQFRASFPPLQT
                     SWTPVTELSVRQEFGPVIVSGRMDLVLGAQDREVPMRARRIALDLKSGRAWPEFPEDM
                     RLYALLMTLRFGVPPFRVASVFLDSGEWQAEDVTEDTLFHAAGRVVRAAEAAAALLAG
                     GEPDLRPGSWCSWCPRAPSCPVAAA"
     gene            1025..2272
                     /gene="ccrA"
                     /locus_tag="VEO00_03775"
     CDS             1025..2272
                     /gene="ccrA"
                     /locus_tag="VEO00_03775"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HYU57154.1"
                     /translation="MVRELYEIGDIPPLGEVPARMYAQVIRPDRFGEPEKAFQVEAIE
                     TPRPGPHDALALVMAAGINYNNVWAARGIPVDVTKVHARYGEPDDYHVGGSDASGVVY
                     AVGERVENVKVGDRVVVHCGQWDPDDPVLAAGEDPMFGGSFRIWGYESNHGSFAQFTK
                     VQAHQCLPKAGHLTWEAAAAPTLVGATAYRMLHGWPPHAVREGDVVLVWGGAGGLGSM
                     AIQLVREAGGRAVAVVSSEEKAEFCRRLGAAGTVNRRDFGHWGPLPDWRDDEAFGEWM
                     VGAKAFGKAFWEALGEKRAPRIVFEHPGEDTVPTSIFLCDTGGMVVICAGTTGYNAVV
                     DLRYLWMRQKRLQGSHFANDEQAGAFNDLVVAGKIDPCLAEPVFAFDEIGAAHQLMHE
                     NRHPEGNMAALVSAPATGLTDLP"
     gene            2453..>2795
                     /locus_tag="VEO00_03780"
     CDS             2453..>2795
                     /locus_tag="VEO00_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57155.1"
                     /translation="MADGVRFSALSTPDDSGSEQLFIVLYDRLDGALNESFGTTLTPG
                     VPREIVAPIPPGLVRMGCFEPPYAEQDVTDAEVAKVEVVDSGGFWGPIELGCATSDLE
                     GLLWIKGEMSKR"
BASE COUNT          375 a          951 c         1056 g          413 t
ORIGIN      
        1 cacgtctacc acaccggggc gtgtcacccc tgccgtctac gatgccggcc gatggatggg
       61 ctgaccccgg tccagcggcg gacgctggac gacctgatgg gcgcgaccgt gccccggccg
      121 gtgttcggcg aggacctggc gggccggctg cgcgccgagg tcgagggagg cgtcgcggcc
      181 ctctcgttcc ccgatccgcc ggtctggctc ggcaagcacc ggctgaacga ccacgggcgg
      241 tgcgaggggc tgttcgtggc cgggctgctg ggcgagggag ctgccttcga acaccggccg
      301 cagacggccg caggcagcct ggcgcacaag gccatcgagc tcgacgtgcc gcgaggccgg
      361 gcggacccca ccccggcgct ggtggagcgg gcggccgccc ggctggccga gggcgacacg
      421 gggttcgccg ccttctggga aggcctcgat ccgctcgagg cgtcggagat ctcccagatg
      481 gcggtgcggc tggccgagca gttccgggcc tcgttcccgc cgctgcagac gtcgtggacg
      541 ccggtcaccg agctgagcgt ccgccaggag ttcggcccgg tgatcgtctc cgggcggatg
      601 gacctggtgc tcggggccca ggaccgcgag gttccgatgc gggcccggcg gatcgcgctc
      661 gacctgaagt cgggccgcgc ctggccggag ttcccggagg acatgcggct ctacgcgctg
      721 ctgatgacgc tccgcttcgg agtgccgccg ttccgcgtgg cgtcggtgtt cctcgactcc
      781 ggtgagtggc aggcggagga cgtcaccgag gacaccctgt tccacgccgc cggccgggtg
      841 gtgcgcgccg cggaggccgc ggcggccctg ctggccggcg gcgagccgga cctgcggccg
      901 gggtcctggt gctcctggtg cccgcgcgcg ccgagttgcc cggtcgccgc ggcctgacct
      961 acccttcggt aggtttccct ccgccgccgc agggaaccca ttcccgtgac ggaggagccg
     1021 cacgatggtg agggaactgt acgagatcgg cgacatccca cccctgggcg aggtccccgc
     1081 ccggatgtac gcccaggtga tccggccaga ccgcttcggg gagccggaga aggccttcca
     1141 ggtcgaggcg atcgagacgc cgcggcccgg gccgcacgac gcgctcgcgc tcgtgatggc
     1201 ggcgggcatc aactacaaca acgtctgggc cgcccggggg atcccggtgg acgtgacgaa
     1261 ggtccacgcc cgctacggcg agcccgacga ctaccacgtc ggcggcagcg acgcgtcggg
     1321 cgtggtctac gcggtggggg agcgggtcga gaacgtgaag gtcggggacc gcgtggtcgt
     1381 ccactgcggg cagtgggacc ccgacgaccc ggtgctcgcc gccggcgagg acccgatgtt
     1441 cggcgggtcg ttccggatct ggggctacga gtcgaaccac ggctcgttcg cccagttcac
     1501 caaggtccag gcgcaccagt gcctgccgaa ggccgggcac ctgacctggg aggcggccgc
     1561 ggccccgacg ctggtcggcg ccaccgccta ccggatgctg cacggctggc cgccgcacgc
     1621 ggtccgcgag ggtgacgtgg tgctcgtctg gggcggcgcc ggcggcctcg gctcgatggc
     1681 catccagctg gtgcgtgagg cgggcggcag ggcggtggcc gtggtgtcga gcgaggagaa
     1741 ggccgagttc tgccgccgcc tcggcgcggc cggcaccgtc aaccgccggg acttcgggca
     1801 ctgggggccg ctcccggact ggcgcgacga cgaggcgttc ggcgagtgga tggtgggggc
     1861 gaaggccttc gggaaggcgt tctgggaggc gctcggcgag aagcgcgccc cgcggatcgt
     1921 gttcgagcac cccggcgagg acaccgtgcc cacctcgatc ttcctgtgcg acaccggggg
     1981 gatggtcgtg atctgcgccg gcaccaccgg ctacaacgcc gtggtcgacc tgcgctacct
     2041 ctggatgcgc cagaagcgct tgcagggctc ccacttcgcg aacgacgagc aggccggcgc
     2101 cttcaacgac ctggtggtgg ccgggaagat cgacccgtgc ctggccgagc cggtgttcgc
     2161 gttcgacgag atcggggcgg cgcaccagct gatgcacgag aaccgccacc ccgaggggaa
     2221 catggccgcc ctggtctcag ccccggcgac cggcctgacc gacctgccct gacgccgtcg
     2281 gcggggagga cggttgatgg tcggatctct ggtcgtcagg ctggggctgg ttctcgttgt
     2341 cgcaactggc tgcggatcgc tctcaagaca ggctgccacc acgaaccctg ctgacgccgc
     2401 ctccgtcacg tgctcctccg atggggtgtc cgctccggct acgatggaca cgttggccga
     2461 tggggtccgc ttctcagcac tcagcacgcc ggatgattca ggctccgagc aactcttcat
     2521 cgtgctctac gatcggctcg acggcgctct gaacgaatcg ttcggcacca cgctcacccc
     2581 tggcgtgccc agggagatcg tggctccgat accaccgggg ctggtgcgta tgggctgctt
     2641 cgagcccccg tacgccgagc aggacgtgac ggacgcggag gtcgccaagg tggaggtcgt
     2701 tgattcgggc ggcttctggg gaccgatcga gctcgggtgt gcaacctcag acctcgaggg
     2761 cctcctttgg atcaagggcg agatgtcgaa acggt
//
LOCUS       DASPWO010000169         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_366155, whole genome shotgun sequence.
ACCESSION   DASPWO010000169 DASPWO010000000
VERSION     DASPWO010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366155"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /gene="rnpA"
                     /locus_tag="VEO00_03785"
     CDS             complement(<1..222)
                     /gene="rnpA"
                     /locus_tag="VEO00_03785"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HYU57156.1"
                     /translation="MIRPVRDRETFDALARARRRAGRGPVSVRFVPGGTTDEPRVAIA
                     TARSTGNAVTRNRVRRRLRAAVAAHQDELV"
     gene            complement(219..353)
                     /gene="rpmH"
                     /locus_tag="VEO00_03790"
     CDS             complement(219..353)
                     /gene="rpmH"
                     /locus_tag="VEO00_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HYU57157.1"
                     /translation="MKRPFQPNNRKRKKKHGFRTRMRTRGGRAVIKSRRRRGRGRLSA
                     "
     gene            693..2090
                     /gene="dnaA"
                     /locus_tag="VEO00_03795"
     CDS             693..2090
                     /gene="dnaA"
                     /locus_tag="VEO00_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HYU57158.1"
                     /translation="MDSRDRGAAAADEIWDEAIFTLRAQLNDAAWSTYFQGVRALAFD
                     GDRLTLGVPSGLAAERIRTAYVPLITEVLTAVAGHKVDAGLVVETDDRVEEPVRVPEP
                     ARVPETPAPDSDPGAWASGKLIPRYTFGQFVIGANNRFAHAAALTVAEAPARSYNPLF
                     IYGDAGLGKTHLLHAVGHHVKAMFPTKRVRYVSTETFMNEFIDSIRMKTMPVFKRRYR
                     DLDMLLMDDIQFLERTQELQEEFFHTFNQLHGDGGQIVISSDRAPKSMDTLEDRLRSR
                     FEWGLSTDIQPPEFETRFAILRKKAEAEHFDNIPDDVLAFISTNITDNVRELEGALIR
                     VAAFAKLNRETALSAEVAHEVLADLLPASKPRVITPELILEETANMFGWTIEDLCGKS
                     RRRPLVTARQIGMYVFRELTDYSYPRIAEQFGGRDHTTVMYAVDKIKRQMPERTAIFT
                     QVNDLISRIKLGTSG"
     gene            2331..>2930
                     /gene="dnaN"
                     /locus_tag="VEO00_03800"
     CDS             2331..>2930
                     /gene="dnaN"
                     /locus_tag="VEO00_03800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HYU57159.1"
                     /translation="MKFRCERDTLADAIATAQRAVAARPGALPVLSGLRLGLGSDGVE
                     LVGSDLELTIRVQAKANVEGEGSAVVPARLLSDIVRKLEPGPVTVEFGDDDARIEGGR
                     ANFALRTLPAADFPRLPEAAGSGVRVEAGALAEALRQVVPAASRDDARPILTGVLLTA
                     SGEGLRLVATDSYRLAVRDLQGVTQFLEEGQKVLVAGRGL"
BASE COUNT          456 a         1072 c          967 g          435 t
ORIGIN      
        1 caccagctcg tcctgatggg cggcgaccgc ggctcggagc cggcgccgga cccggttgcg
       61 cgtcacggcg ttgcccgtcg agcgggcggt cgcgatcgcg acccggggct cgtcggtggt
      121 cccgccgggg acgaagcgaa cactgaccgg gccccgaccg gcccgacggc gcgctcgggc
      181 gagggcatcg aaggtttcac gatcgcggac gggccggatc aggccgagag ccggccgcgt
      241 ccccgccgcc gccgggactt gatgacggcc cggccgccac gggtgcgcat gcgggtgcga
      301 aaaccgtgct tcttcttgcg cttccggttg ttcggttgaa acgggcgctt cacccatggc
      361 ctcctcgcgc cggcccgctc gtgacccgag gtgggggatc accgcagagg aagtgggccg
      421 gaagcccgta agtgtacggg cgcaacacgc gcacccgcaa cgcgcccgcc ggcgcgcgtt
      481 gtggacgcct ccaccggcgc gccgttttcg cagtcggcgg aacgtgcacg cgccccttgt
      541 cgggtgggga tgccggtggt acgttccgcg ctcccgccct tcgtcacggc ctcgaaggcg
      601 gacttccccg agcccagctc aaccaggccg ccagccgggc ggaggggacc aaccggtccg
      661 ctccatgttc ttccacacct gtggatgttc ctgtggacag tcgagatcgg ggcgcggcag
      721 cggccgacga gatctgggac gaggcgattt tcacgttgcg cgcccagctc aacgacgcgg
      781 cctggagcac ctacttccag ggcgtccgcg cgctcgcgtt cgacggcgac cgtctgaccc
      841 tgggcgttcc gagcggcctg gcggctgagc gcatccggac cgcctacgtc ccgctcatca
      901 ccgaggtcct cactgcggtg gcgggccaca aggtcgacgc cgggctcgtc gtcgagaccg
      961 acgaccgcgt cgaggagccc gttcgcgtcc ccgagccggc gcgggtcccg gagacgccgg
     1021 cgcccgactc cgacccgggc gcgtgggcct cggggaagct cattccccgc tacacgttcg
     1081 gccagttcgt gatcggagcc aacaaccgct tcgcccacgc cgccgccctc accgtcgccg
     1141 aggcgccggc gcggtcgtac aacccgctgt tcatctacgg cgacgccggc ctcggcaaga
     1201 cccacctgct gcacgcggtc ggtcaccacg tcaaggcgat gttcccgacc aagcgcgtcc
     1261 gttacgtctc gaccgagacg ttcatgaacg agttcatcga ctcgatccgc atgaagacga
     1321 tgccggtgtt caaacgccgg taccgcgacc tcgacatgct cctgatggac gacatccagt
     1381 tcctcgagcg gacccaggag ctccaggagg agttcttcca caccttcaac cagctccacg
     1441 gcgacggtgg ccagatcgtg atctcgtccg accgggcacc gaagtcgatg gacaccctgg
     1501 aggaccggct gcgcagccgc ttcgagtggg ggctcagcac cgacatccag ccgcccgagt
     1561 tcgagacccg cttcgccatc ctgcgcaaga aggccgaggc cgagcacttc gacaacatcc
     1621 ccgacgacgt cctcgccttc atctccacca acatcaccga caacgtccgc gagctcgagg
     1681 gcgcgctcat ccgggtggcc gcgttcgcca agctgaaccg cgagacggct ctgtcagccg
     1741 aggtcgccca cgaggttctc gccgacctcc tcccggcgtc gaagccccgg gtcatcacgc
     1801 ccgagctcat cctcgaggag accgccaaca tgttcggctg gacgatcgag gacctctgcg
     1861 gcaagagccg ccgccggccg ctggtcaccg cccgccagat cggcatgtac gtcttccggg
     1921 agctgaccga ctacagctac ccccgcatcg ccgagcagtt cgggggccgg gaccacacga
     1981 cggtcatgta cgcagtcgac aagatcaagc ggcagatgcc cgaacgcacc gccatcttca
     2041 cccaggtcaa cgacctcatc agccggatca agctcggcac cagtggataa ggctgtggaa
     2101 gcctggggat tccccccggg ttctggggaa aaccaccgtt ccctccgctc ccgtcccatc
     2161 tcccctgtgg acaacggtgg agaaccggtg cactgggatc ggcgctccca ccagcaccgg
     2221 aatccacttg tcctcaatcc acaggcccta ctactacgac tatcaatgaa gagtagttct
     2281 ccgtaggaca agatccccag gaccgacaca cccttcgaga gaggacccgg ttgaagttcc
     2341 gttgcgagcg cgacaccctc gccgacgcca ttgccaccgc gcagcgggcg gtcgcggccc
     2401 gaccgggcgc gctcccggtg ctgtcggggc tgcggctggg cctggggtcc gacggggtcg
     2461 agctggtcgg ctccgacctc gagctcacga tccgggtcca ggccaaggcc aatgtcgagg
     2521 gcgaaggcag cgccgtcgtg cccgcccgcc tcctgtccga catcgtccgg aagctggagc
     2581 cgggtccggt caccgtcgag ttcggggacg atgacgcccg catcgagggc ggtcgggcga
     2641 acttcgcgct gcgcacgctg ccggcggcgg acttcccccg cttacccgag gcggcggggt
     2701 cgggcgtccg ggtcgaagcc ggggccctgg ccgaggcgct gcggcaggtc gtgccggcgg
     2761 cctcgcgcga cgacgctcgg ccgatcctca ccggcgttct gctcaccgcc tcgggcgagg
     2821 gattgcgcct ggtcgccacc gactcgtacc ggctcgcggt gcgcgatctc cagggcgtca
     2881 cccagttcct cgaggagggc cagaaggttc tggttgcggg gcgcggcctc
//
LOCUS       DASPWO010000170         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_366368, whole genome shotgun sequence.
ACCESSION   DASPWO010000170 DASPWO010000000
VERSION     DASPWO010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366368"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VEO00_03805"
     CDS             <1..673
                     /locus_tag="VEO00_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU57160.1"
                     /translation="TGGLDRASAGEVLVEGRDLGRLGDEELTRYRRDMVGFVWQGTAR
                     NLVPYLSLRENVRLPLLAQGQRAHRSRIEELLGVVGLAERARHRPNELSGGEQQRAAI
                     AVALANMPPLLLADEPTAELDTASADRVLSAFRDVNREFGSTIVMVTHDLVAAARGDR
                     MVRVRDGRLAREALVIEGPAQDGQMHLPDQAVRLLRGGEVEAEVQDDEVRLRRRHERG
                     QGRRG"
     gene            666..1427
                     /locus_tag="VEO00_03810"
     CDS             666..1427
                     /locus_tag="VEO00_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU57161.1"
                     /translation="MAEPGPPTDVSVVSAPAGVPLLAAHDVVRRYPGRVSVLAVRGAS
                     VALWPRDFVALMGPSGSGKTTFLGVLAGLDQPDTGEVRWRGELLGALGGQALVDLRQR
                     RMGIVFQSFGLLPALSAVENVELPLRVSGETPRLARERAAEWLRRLRLGDRLDNRTFE
                     LSAGQQQRVAVARALITEPDVVLADEPIAEVDTENADIILEALWDVVRRGGAVLAATH
                     DPEALRYANRVVLFRDGVVEADGPVDEVAGHLTTD"
     gene            complement(1461..1964)
                     /locus_tag="VEO00_03815"
     CDS             complement(1461..1964)
                     /locus_tag="VEO00_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57162.1"
                     /translation="MGIVLGALVVPRVASAVGSFVTIQGPGGGNKATVTKPNQLEVAE
                     APPSQFREFTNVSTDSACHPLATIPGNKGFVARSATLVVLTAASTGFPIAEVFPNGSC
                     AGSDVASTTTGNTGNRTMTLEPGFAIAPGGHMSMKLGTTGAVVAAYVFGYLVPAGDVP
                     ATTPIGS"
     gene            2285..>2570
                     /locus_tag="VEO00_03820"
     CDS             2285..>2570
                     /locus_tag="VEO00_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57163.1"
                     /translation="MRKVWTLALGAGLAMTLAACSSGGGGDGTATCTPHGPALRITAR
                     NVSFDTDCLAAPADRPLTIAFDNEDGGTPHNLSISSDAASDALFTGERFSG"
BASE COUNT          344 a          946 c          947 g          333 t
ORIGIN      
        1 gaccggaggc ctcgaccggg cctcggccgg ggaggtcctg gtggaggggc gcgacctcgg
       61 ccgcctcggc gacgaggagc tcacccgcta ccgccgcgac atggtcgggt tcgtgtggca
      121 gggaaccgcc cgcaacctgg tcccctacct gagcttgcgc gagaacgtgc ggctgcccct
      181 cctggcccag ggacagcggg cccaccgcag ccgcatcgag gagctgctgg gggtggtggg
      241 gctcgccgaa cgggcccgcc accgcccgaa cgagctctcc ggcggcgagc agcagcgggc
      301 cgccatcgcg gtggccctcg cgaacatgcc gcccctgctg ctggcggacg agcccacggc
      361 ggagctcgac accgcgtcgg cggatcgggt actcagcgcg ttccgcgatg tcaatcggga
      421 gttcggctcc acgatcgtca tggtgaccca cgacctggtg gcggcggccc ggggcgaccg
      481 gatggtgcgg gtgcgcgacg gtcggctggc tcgcgaggcc ctggtgatcg agggcccggc
      541 ccaggacggg cagatgcacc tccccgacca ggccgtgcgg ctcctgcggg gcggcgaggt
      601 cgaggccgag gtgcaggacg acgaggttcg gttgcggcgg cgacacgaac gcggccaggg
      661 tcgtcgtggc tgagcccggg cccccgacgg acgtgtcggt ggtatccgcg ccggcgggcg
      721 tcccgctgct cgccgcgcac gacgtggtcc ggcggtaccc cggccgagtg agcgtcctgg
      781 ccgtccgggg ggcttccgtc gcgttgtggc cgagggactt cgtggcgctg atggggccct
      841 cggggtccgg caagaccacg ttcctcggcg tcctcgcggg gctcgaccag cccgacaccg
      901 gcgaggtccg gtggcgaggc gagctcctcg gagcgctcgg cgggcaggcc ctggtcgacc
      961 tccggcagcg ccggatgggc atcgtcttcc agagcttcgg gctcctgccg gcgctgtccg
     1021 cggtggagaa cgtcgagctg ccgttgcgcg tctcgggcga gacccctcgc ctggcccggg
     1081 aacgcgccgc cgagtggctg cggcgcctcc gcctcggcga ccgactggac aaccggacgt
     1141 tcgagctctc cgcgggccag cagcagcgag tcgccgtcgc gcgggcgctc atcaccgagc
     1201 cggacgtcgt cctggcggac gaacccatcg ccgaggtcga caccgagaac gccgacatca
     1261 tcctggaggc gctgtgggac gtggtccggc gggggggcgc cgtgctggcc gccacgcacg
     1321 accctgaggc gctgcgctac gcgaaccgcg tggtgctgtt ccgcgacggc gtggtggagg
     1381 ccgacggccc ggtggacgag gtcgccggcc acctcaccac cgactgaccc ggttcgacgc
     1441 caccggcgcc caggtggggt tcagctcccg atcggcgtgg tggccgggac gtcgccagcc
     1501 gggacgaggt acccgaacac gtaggcggcc acgaccgcgc ccgtcgtgcc gagcttcatc
     1561 gacatgtgac cacccggagc gatggcgaaa cccggctcca gcgtcatcgt gcggttgccg
     1621 gtgttccccg tggtggtcga cgccacgtcc gaccccgcgc acgaaccgtt cgggaagacc
     1681 tcggcgatcg ggaacccggt ggaggccgcg gtgagcacca cgagcgtcgc cgagcgcgcc
     1741 acgaacccct tgttgccggg gatcgttgcg agcggatggc acgccgagtc ggtggagacg
     1801 ttcgtgaact cgcggaactg ggagggcggc gcctcggcca cctccagctg gttcggcttc
     1861 gtcacggtgg ccttgttccc cccgccgggc ccctggatcg tcacgaagct ccccacggcg
     1921 ctcgcgaccc gcggcaccac cagagcgccc agcacgatcc ccatcaccag cacctacgcc
     1981 cgaccgcgtc ccgcccgttc catccgccgc cctcctcacg cacccgcggg cccatcgccc
     2041 gcgctcggag gcggtcatac accggaacgc cctgcggcgc agctcggccg gcccctgccg
     2101 ggcgggcacc gcgccgcacg cgacgcggtc acacaccagc cgcacatgcg gaagccaacc
     2161 tcgccccggt cgaatcgcct cgccgtggtc gaatccggcg gccgatcggt aggctgggtc
     2221 gccctcggcg cccggctgtc catacgcgca gccggcacgc gtcaagcaac gaaaggcaca
     2281 tccgatgcga aaggtctgga cgctcgcgct cggcgcggga ttggcgatga cgctggccgc
     2341 atgcagcagc ggtggcggag gggacgggac ggcgacgtgc acgccgcacg gcccggccct
     2401 ccggatcacg gcgaggaacg tctcgttcga caccgactgt ctggcggcgc cggccgatcg
     2461 gccgctcacg atcgcgttcg acaacgagga cggcgggacg ccgcacaacc tctcgatctc
     2521 ctcggatgcc gcgtcggacg cgctgttcac gggcgagcgc ttctccggcg
//
LOCUS       DASPWO010000171         5136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_536300, whole genome shotgun sequence.
ACCESSION   DASPWO010000171 DASPWO010000000
VERSION     DASPWO010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536300"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..439)
                     /gene="rnpB"
                     /locus_tag="VEO00_03825"
     ncRNA           complement(56..439)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VEO00_03825"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(695..823)
                     /locus_tag="VEO00_03830"
     CDS             complement(695..823)
                     /locus_tag="VEO00_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57164.1"
                     /translation="MTVETYTIRGTPAATAASSTFSVPVTFVADIPGRSRCGMPIV"
     gene            964..1476
                     /locus_tag="VEO00_03835"
     CDS             964..1476
                     /locus_tag="VEO00_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57165.1"
                     /translation="MIVQPGVVYGPGDRSQTGGQFERAALGKLPFLSFPEVGFSLAHV
                     DDVAEGILLAHDRGEIGHSYVFAGNVTRMREAIETIAAAGGTRPPRFAMPTWAMEASI
                     PFGRIVGKVMGVPPNLREIVSASDGVTYWASSDRARRELGYRPRDMRDGLAELARDIL
                     GAHGRTPGAR"
     gene            complement(1491..1994)
                     /locus_tag="VEO00_03840"
     CDS             complement(1491..1994)
                     /locus_tag="VEO00_03840"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HYU57166.1"
                     /translation="MSQLPADPGPHLIVSADGAARGNPGPAGIGAVVTTPRGRVLAEV
                     AEGIGIATNNVAEYRAALAGLQRARDLGASRVTFRSDSRLLIEQLAGRYRVKNPTLQR
                     LHGEVGDVLREIPEVDLVHVPRAENVHADRLANVGVDAWLAGAGRRWRRSRPNPTLFD
                     PQPPDRP"
     gene            complement(1958..2683)
                     /locus_tag="VEO00_03845"
     CDS             complement(1958..2683)
                     /locus_tag="VEO00_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HYU57167.1"
                     /translation="MNDGEAPATDPLLDLQELDLAVDRLEHRRAELEGGEEVRAARRQ
                     ADELEERLGGLRLALDSVGREQTRLDTEIDSFSRKMADEEKRLYDGSVANPRELEAIR
                     HELTNLANRKRRVEDELLDQMEKREQLEHRIVPLEGELAEARERLGELAGHAEVELAE
                     VVASLVERRAERDALRPRLDEDLVELYDDLRAQKKGIGAAALEGRVCQACRTQLSALE
                     YEQVKKADGIRRCPNCRRILVLT"
     gene            complement(2730..>5136)
                     /locus_tag="VEO00_03850"
     CDS             complement(2730..>5136)
                     /locus_tag="VEO00_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYU57168.1"
                     /translation="AVAAQRDLAGHGWSPGPELRVRMGLHTGEGSLGGEDYLGIDVNR
                     AARIADAAHGGQVIVSDATRSLVERDLPAGVSLRGLGEHRLKDLVHPERLHDLVIEGL
                     TSDFPPPRTLDARPNNLPVQLTSFIGREDEIAEVRGLLGRTRLLTFTGPGGTGKTRLA
                     LQVASEVLTEYADGAFFADLSSVTDPALVPSALAGALGVAEVPGRPMLEAVESHLRDR
                     EILLVVDNFEQVADAGPVIEELLTTAPRLTVLVTSRLVLGLRGEQEYAVPPLQPPDPE
                     RLPDLLTLRRFEAVRLFAERALAADPRFEVTEENAPAVAEITARLDGLPLAIELAATR
                     TKLLSPDQMLARLDQRLDILTSGGRTLPERQRTLRNAIAWSYDFLDEPQRRLFDRLSV
                     FTAGWTLESAETVCDLEQLGLDILDGLTALVDQSLVRRSDQGRGDPRFSMLETIREYG
                     LERLGAGGDLDAVRRRHAEHFLGLAAEAEPHLTADDQGAWLDRCDLEHANIRAALRWA
                     IEAGEADRAQEAAAALWRFWQQRGHLAEGRGWFGEVLGMPGGRGRTRFRARALIGAGG
                     IEWWQGDRVAAGAFYEEALAIERELGDPIRTAEALYNLAFVVAGDDLEASTRMLEESR
                     DLFRRAGDERGVAQVLTMLVMQDAQAGHWDRVIASLEEVVATWRRVGDRLHLAFDLVW
                     LAFAYGRIGRRAEAWSTALESLDLFREVDNPTGIGIALIDLAFLATWEGRHLDAIKLA
                     AAYESMRERVGGPPGAIGGLLEGDPAAEARLHVSEESAQRAWEDGQAMTVDQAVALAR
                     GGS"
BASE COUNT          664 a         1948 c         1813 g          711 t
ORIGIN      
        1 gggggaggcc gtcgcaaccc aaggagacgc cagccggagg agcggggggg agaggtggag
       61 gagccggcct gtaagccgga ttctgtcccc gcgcccggcg ggcgcgggtg gcgaccatcc
      121 ctctgggggc cgcgtcaccg cgaccctcgg tgcggcctac ccgggagctc ggacgggccg
      181 tcctcgagcg ctccctgctc ggccttgctc cggatggggt ttaccgagcc gggacggtct
      241 cccgccccgc tggtgagctc ttacctcacc gtttcaccct tgcccttacc ctcggcggcg
      301 agccgtcgtc gggcagtggg cggtctgctc tctgtggcac tgtccgcggg tctccccgcc
      361 cgggcgttac ccggcatcct gccctgcgga gtccggactt tcctcgaccc tctcgggccg
      421 cggccgcccg gccgactcct cctcgacgag tatacggcgc tacagtcccg cccatgcgcg
      481 ccttcgtcac gggcgccacc gggttcatcg gcggcgctct cgcccggaag ctccgcgagc
      541 gaggggacgc ggtggcgtgc ctcgttcgct cgccggatcg cgccacggcg ctggcggacc
      601 tcggctgcga gctgatccgc ggcgacgtcc agagcgccga ccggcacgtg gaggacctgc
      661 ggggccgcga cgccgtcttc catctggcgg ccatctacac gatcggcatc ccgcagcggg
      721 agcggccggg gatgtccgcc acgaacgtca ccgggaccga gaaggtgctc gatgcggccg
      781 tcgcggcggg ggttccacgg atcgtgtacg tctcgacggt caacgcgttc ggcaacaccc
      841 gcggacgggt cgtcgacgag gagtaccgcc gcccggacgg cccgttcgtc tcctactacg
      901 acgagacgaa aataccgcgc tcacctcgcc gccgaggagc gcatcgcgcg tggcgcgccg
      961 atcgtgatcg tccagccggg ggtggtctac gggccgggcg accggtccca gacgggcggg
     1021 cagttcgagc gcgcggcgct cgggaagctc ccgttcctct ccttcccgga ggtcgggttc
     1081 tcgctggcgc acgtcgacga cgtggccgag ggcatcctgc tcgcccacga ccgcggcgag
     1141 atcggccact cctacgtctt cgccgggaac gtcacgcgga tgcgggaggc catcgagacg
     1201 atcgcggccg ccggagggac ccgaccgccg cgcttcgcga tgcccacctg ggcgatggag
     1261 gcctccatcc cgttcgggcg gatcgtcggc aaggtgatgg gggtccctcc gaacctgcgg
     1321 gagatcgtct cagcatccga cggggtgacc tactgggcct cgtcggaccg cgcccgccgc
     1381 gagctcggct accgtccgcg cgacatgagg gacggcctgg ccgagctggc gcgggacatc
     1441 ctgggcgccc acggccgcac accgggggcg cgctaacccc gggcacctgc tcaggggcgg
     1501 tccggcggct gcggatcgaa gagcgttggg ttcggccggg aacggcgcca ccttcggccg
     1561 gcgccggcca gccacgcgtc cacgccgacg ttcgccagcc ggtcggcgtg aacgttctcc
     1621 gcgcgcggaa cgtgcaccag atcgacttcc gggatctcgc gcaggacgtc gccgacctca
     1681 ccgtgcagcc gctgcagggt cgggttcttg acccggtaac ggccggcgag ctgctcgatc
     1741 agcaggcggc tgtccgaccg gaaggtcacc ctcgaggcgc cgaggtcccg ggcgcgctgc
     1801 aaaccggcga gcgcggcgcg gtactcggcg acgttgttcg tggcgatccc gatcccctcc
     1861 gccacctcgg ccagcacccg gcccctcggc gtggtgacca ccgcgccgat ccccgccgga
     1921 ccggggttcc cgcgcgcggc cccgtcggcg ctgacgatca ggtgaggacc aggatccgcc
     1981 ggcagttggg acacctccgg atgccgtcgg ccttcttcac ctgctcgtac tccagggccg
     2041 agagctgggt ccggcaggcc tggcagactc ggccctcgag cgccgcggcg ccgatcccct
     2101 tcttctgcgc ccgcaggtcg tcgtacagct cgacgaggtc ttcgtcgagc cgaggccgga
     2161 gcgcatcccg ttcggcgcgc cgctcgacga gggatgccac gacctcggcc agctccacct
     2221 ccgcgtgccc ggcgagctcc ccgagccgct cccgggcctc ggccagctcc ccctcgagcg
     2281 gcacgatcct gtgctcgagc tgctcgcgct tctccatctg gtcgagcagc tcgtcctcta
     2341 ctcgcctctt gcggttcgcc aggttggtca gctcgtgccg gatcgcctcc agctctcgcg
     2401 gattggccac cgacccgtcg tagagccgct tctcctcgtc ggccatcttg cgggagaacg
     2461 agtcgatctc ggtgtccagc cgggtctgct cgcgccccac cgagtcgagg gccaggcgca
     2521 gcccgccgag acgctcctcc agctcgtcgg cctgtcgccg ggcggcgcgc acctcctccc
     2581 cgccctccag ctcggcgcga cgatgctcca gacggtcgac ggccaggtcg agctcctgca
     2641 ggtcgaggag cgggtcggtg gccggtgcct cgccgtcgtt catcgccggc acagggtacc
     2701 cggctcgagc cgaacgccgc cttccgggct cacgagcctc cccgcgccag ggccacggcc
     2761 tggtcgacgg tcatcgcctg gccgtcctcc caggcgcgct gcgccgactc ctccgacacg
     2821 tggagccgag cctcggccgc gggatccccc tccaggagcc cgccgatggc gccggggggg
     2881 ccgccgaccc gctcgcgcat ggattcgtat gccgccgcga gcttgatggc gtccaggtgg
     2941 cgcccctccc aggtcgccag gaacgccaga tcgatgaggg cgatgccgat gcccgtcggg
     3001 ttgtccacct cgcggaagag gtccagggac tcgagcgcgg tggaccacgc ttccgcccgg
     3061 cgaccgatgc ggccgtaggc gaaggccagc cacaccaggt cgaacgcgag atggaggcga
     3121 tccccgaccc gccgccaggt ggccacgacc tcctcgaggc tcgcgatgac acggtcccag
     3181 tgtccggcct gggcgtcctg catcacgagc atcgtcagga cctgcgccac cccgcgctcg
     3241 tcccccgccc ggcgaaacag gtcacggctc tcctccagca tccgggtgct ggcttcgagg
     3301 tcgtcgccgg cgacgacgaa cgccaggttg tacagggcct ccgcggtccg gatggggtcg
     3361 ccgagttccc gctcgatggc cagcgcctct tcgtagaacg cgccggccgc cacgcggtcc
     3421 ccctgccacc actcgatccc cccggcgccg atcagagccc gggctctgaa ccgggtccgg
     3481 ccccggccgc ccggcatccc caggacctca ccgaaccacc cccggccttc ggcgaggtgc
     3541 ccgcgctgct gccagaaccg ccacagggcg gccgccgcct cctgggcccg gtcggcttcg
     3601 ccggcctcga tggcccaccg gagcgccgcg cggatgttgg cgtgctccag gtcacatcgg
     3661 tcgagccagg cgccctgatc gtcggcggtg aggtgcggct cggcctcggc ggccaggccg
     3721 aggaagtgct cggcgtggcg ccggcgcacg gcgtcgaggt ccccccccgc cccgagccgc
     3781 tccaggccgt actcccggat cgtctcgagc atggagaaac gaggatcacc ccggccctgg
     3841 tcgctccggc ggaccaagga ctggtccacc agcgcggtca acccgtcgag gatgtccagc
     3901 ccgagctgct cgaggtcgca caccgtctcc gccgactcga gggtccaccc ggctgtgaac
     3961 accgacagcc ggtcgaacag acgacgctgc ggctcgtcga ggaagtcgta gctccaggcg
     4021 atggcgttgc gcagggtccg ctggcgctcc ggcaaggtcc ggccccccga ggtgaggatg
     4081 tcgagccgct ggtcgagccg ggcgagcatc tggtcgggcg acaggagctt cgtccgggtg
     4141 gccgccagct cgatggccag gggcaggccg tccagccggg cggtgatctc cgccacggca
     4201 ggcgcgttct cctccgtcac ctcgaaccgc ggatcggccg ccagggcccg ctcggcgaac
     4261 agccggaccg cctcgaaccg acggagggtg aggaggtcgg gcagccgctc ggggtcgggc
     4321 ggctgaagcg gcggcaccgc gtactcctgc tcgccgcgca gaccgagcac cagacgggac
     4381 gtcaccagga ccgtgagccg cggggcggtg gtcaggagct cctcgatcac cggccccgcg
     4441 tcggcgacct gttcgaagtt gtcgaccacc aggaggatct ctcggtcgcg caggtgggac
     4501 tccaccgcct ccagcatggg gcgcccgggc acctcggcga cccccagggc gccggccagc
     4561 gccgagggga cgagggcggg atcggtcacc gacgacaggt cggcgaagaa ggccccgtcc
     4621 gcgtactcgg tcaggacctc cgaggccacc tgcagggcga gccgcgtctt cccggtcccg
     4681 ccgggaccgg tgaacgtgag gagccgggtc cgccccagca gcccccggac ctcggcgatc
     4741 tcgtcctccc gcccgatgaa ggaggtcagc tggaccggga ggttgttcgg ccgggcgtcc
     4801 agggtgcgcg gcggcgggaa gtccgacgtg agcccctcga tcaccaggtc gtggaggcgc
     4861 tcggggtgga cgagatcctt caaacggtgc tccccgaggc cccgcagcga gacccccgcg
     4921 gggaggtcgc gctccacgag gctgcgggtg gcgtcggaga cgatgacctg ccccccgtgg
     4981 gccgcgtctg cgatccgcgc ggcgcggttc acgtcgatgc ccaggtagtc ctctccgccc
     5041 agggacccct cgcccgtgtg cagccccatc cggacccgca gctcggggcc gggcgaccag
     5101 ccgtgcccgg ccaggtcccg ttgggccgcc accgcg
//
LOCUS       DASPWO010000172         3766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_26442, whole genome shotgun sequence.
ACCESSION   DASPWO010000172 DASPWO010000000
VERSION     DASPWO010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26442"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VEO00_03855"
     CDS             <1..289
                     /locus_tag="VEO00_03855"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393009.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HYU57169.1"
                     /translation="RAVEALDRRVPEVLQALAGDGLLFLTGDHGCDPTTPSTDHSRER
                     TPCLVAGLAERPPVTFGDRRFSDLGATIARALGVRTDGLAGESFAADLGLG"
     gene            289..1602
                     /locus_tag="VEO00_03860"
     CDS             289..1602
                     /locus_tag="VEO00_03860"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HYU57170.1"
                     /translation="MDFRDVVAAKRDRRPVPADDLRAFVLAYARGEVPDYLAAAFLMA
                     AYIHPLDTEETLAYTRAMVDSGVTLSFPAAGRPTIDKHSTGGVADGVTLVFVPLAAAL
                     GLAVAKLSGRGLGHTGGTLDKLESIPGFRTDLSGEEIERQVAEVGCVVAAQTKDLVPA
                     DGALYALRDATATVSSVPLIAASVMSKKLATLTDLILLDVKSGSGAFMKTAEDAAALA
                     NACVDLARAWGRRCRAAVTDMSQPLGNAVGNALDVAEAVEALRGERRGRLTDLAVAFA
                     GAALRSLDGAEDGEVRAATALRDGTALSAFARMVEAQGGDPGVADDPWSVLPRAPVVL
                     PIEAPRGGVLAAVDAEEIGRAAVDLGAGRVRKGDPVDPAVGIVFEPKVGDRVEAGRVG
                     EVHARDEDAARAAAARVLAACTLADESDEPVAAPALLLGWHGTED"
     gene            1603..2250
                     /locus_tag="VEO00_03865"
     CDS             1603..2250
                     /locus_tag="VEO00_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYU57171.1"
                     /translation="MSDNVKFALYLAVALVPSLVLHERAHAWWAGRLGDLTPKAFGRA
                     TLNPRAHVDPFGSIVLPLFLLVLWASGRLLPPVFAYAKAMPLNPQNMKDPARGPLQVA
                     LAGMAVNLALAVLAALAVRAFGLSEAGLVAVAFLHVNVVMFVFQLMPVPGLDGSVILS
                     RFLRGRAREVYGNLDQYLPLFMLVIFFLLSAPLLTIVNALGNSLCGILVGFDCLS"
     gene            2282..3352
                     /gene="trpS"
                     /locus_tag="VEO00_03870"
     CDS             2282..3352
                     /gene="trpS"
                     /locus_tag="VEO00_03870"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HYU57172.1"
                     /translation="MGRRARAIIGRIDQVTENPTGAGTGPRRARVLTGYRPTGPLHLG
                     HWFGNIQNMLRLQLEHEAFFFVADWHALTTDFDRTENLPGNVRGLILDWLAAGIDPEV
                     ATVYRQSDLPEVAEMTLLIGMIVPLGWLERVPTFKERLRDLSEREIANYGLLGYPVLQ
                     TVDITIVRGELVPVGEDQLPHLELSREIVRRFNKLYGPVLVEPKALLSESPQIPGIDG
                     RKMSSSLGNDIRLRDDPDTVRKKVRRMVTDPQKVRLGDPGRPEICPVFALHRRFSEDI
                     LDETAAGCRAGTLPCVQCKENLADRLIPALGPYREARERLETTPGVAGEVLADGAKRV
                     RPVVEETIDAVRTAMRMKGLGE"
     gene            3349..>3766
                     /locus_tag="VEO00_03875"
     CDS             3349..>3766
                     /locus_tag="VEO00_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HYU57173.1"
                     /translation="MSRAEGVRAEPGAFAVETPVFTGPFRALAELLLGRKVDVCDVPL
                     RGVVDAFVALAPEAGGRTLEEQTWFVVTCATLLEMKVARLTPRDIEPDEEDLVGGSPD
                     LAYARRLELSAFREAAREVARWLEDGGRRFGRGSGPG"
BASE COUNT          465 a         1332 c         1435 g          534 t
ORIGIN      
        1 gcgggccgtc gaggcgctcg accggcgcgt gcccgaggtg ctccaggcgc tggccggcga
       61 cgggctcctg ttcctgaccg gcgaccacgg ctgcgacccg acgacgccct ccaccgacca
      121 ctcccgcgag cgcacgccgt gcctggtggc cggcctcgcc gagcggcccc cggtgacctt
      181 cggcgaccgc cgcttctccg acctcggcgc gacgatcgcg agggcgctcg gcgtgcgcac
      241 cgacggcctg gccggcgaat cgttcgcggc cgacctcggg ctcgggtgat ggatttccgt
      301 gacgtcgtcg ccgccaagcg cgatcggcgt ccggtgccgg ccgacgacct gcgggccttc
      361 gtgctggcgt acgcgcgcgg cgaggtgccc gactacctgg ccgcggcgtt cctgatggcc
      421 gcctacatcc acccgctcga tacggaggag acgctcgcct acacgcgggc gatggtggac
      481 tccggcgtca cgctgtcgtt cccagccgcc ggccggccga cgatcgacaa gcactccacc
      541 ggcggcgtgg cggacggggt gacgctcgtg ttcgtgccgc tcgccgcggc cctcggtctg
      601 gcggtggcaa agctctccgg gcgggggctg gggcacacgg gcggcacgct cgacaagctc
      661 gagtcgatcc ccgggttccg caccgacctc tccggcgagg agatcgagcg gcaggtcgcg
      721 gaggtcggtt gcgtcgtcgc ggcgcagacg aaggacctgg tcccggccga cggcgcgctg
      781 tacgcgctgc gcgacgcaac cgccacggtc tcgtcggtcc cgctgatcgc ggcgagcgtg
      841 atgtcgaaga agctggccac cctgaccgac ctgatcctgc tcgacgtgaa gagcgggagc
      901 ggcgcgttca tgaagacggc cgaggacgcc gccgccctgg cgaacgcctg cgtcgacctg
      961 gcgcgggcgt ggggacgtcg ctgccgggcg gccgtcaccg acatgtccca gccgctcggg
     1021 aacgcggtcg ggaacgcgct cgacgtcgcg gaggccgtcg aggcgctgcg cggggagcgc
     1081 cggggacggc tgacggacct ggcggtggcg ttcgccggcg cggcgctgcg ctcgctggac
     1141 ggggccgagg acggcgaggt ccgcgccgcg accgccctcc gggacgggac cgccctctcg
     1201 gccttcgccc ggatggtcga ggcacaggga ggcgacccgg gggtggccga cgacccgtgg
     1261 tcggtgctgc cgcgggcgcc ggtggtgctg ccgatcgagg ctccgcgcgg cggggtgctc
     1321 gcagccgtgg acgccgagga gatcgggcgg gccgccgtcg acctcggcgc cggccgggtg
     1381 cgcaagggcg acccggtcga tccggcggtc ggcatcgtct tcgagccgaa ggtgggggac
     1441 cgggtggagg ccggccgggt gggcgaggtg cacgcgcggg acgaggacgc agcccgcgct
     1501 gccgcggccc gggtgctcgc cgcgtgcacg ctcgcggacg agtcggacga gccggtggcg
     1561 gcgcccgcgc tcctcctcgg ctggcacggg acggaggact gagtgtcgga caacgtgaag
     1621 ttcgcgctct atcttgcggt ggccctggtc ccgagcctgg tcctgcacga gcgcgcgcac
     1681 gcctggtggg ccgggcggct cggcgacctg accccgaagg ccttcggccg ggcgacgctc
     1741 aacccgcgcg cccacgtcga ccccttcggc tcgatcgtcc tgccgctgtt cctgctcgtc
     1801 ctgtgggcct ccggacgcct gcttcccccg gtgttcgcct acgcgaaggc catgccgctc
     1861 aacccgcaga acatgaagga ccccgcgagg gggccgctcc aggtggcgct ggccgggatg
     1921 gcggtcaacc tggcgctcgc cgtcctcgcg gcgctcgcgg tccgcgcgtt cgggttgagc
     1981 gaggcagggt tggtcgctgt ggccttcctg cacgtgaacg tcgtgatgtt cgtcttccag
     2041 ctgatgcccg tcccggggct cgacgggagc gtcatcctgt cgcgcttcct gcgcgggcgg
     2101 gcacgcgagg tgtacgggaa cctcgaccag tacctgccgc tgttcatgct ggtgatcttc
     2161 ttcctgctct cggcgccgct gctcacgatc gtcaacgcgc tgggcaactc gctgtgtggg
     2221 atcctggtgg ggttcgactg cctctcgtga ggagcgcctt gggaggaggt cgcgctcccg
     2281 ggtgggccgg agagcccgcg ctatcatcgg gcgcatcgac caggtcaccg agaaccccac
     2341 cggcgccggg accgggcccc gacgagcgcg ggtcctgacc ggctaccgac cgaccggccc
     2401 cctgcacctc gggcactggt tcgggaacat ccagaacatg ctccggctcc agctggagca
     2461 cgaggcgttc ttcttcgtcg ccgactggca cgcgttgacc acggacttcg accgcaccga
     2521 gaacctcccc gggaacgtcc gcggcctgat cctcgactgg ctggcggcgg gcatcgaccc
     2581 ggaggtggcg accgtctacc ggcagtcgga cctgccggag gtcgccgaga tgacgctcct
     2641 gatcgggatg atcgtcccgc tcgggtggct cgagcgggtc ccgacgttca aggagcggct
     2701 gcgcgacctg tccgagcgcg agatcgcgaa ctacgggctg ctcggctacc cggtgctgca
     2761 gaccgtggac atcacgatcg ttcggggcga gctggtgccg gtgggcgagg accagctgcc
     2821 gcacctcgag ctctcccgcg agatcgtccg ccggttcaac aagctgtacg gcccggtgct
     2881 ggtggagccg aaggccctgc tgtccgagag tccgcagatc ccggggatcg acggccgcaa
     2941 gatgtcctcc tccctgggca acgacatccg gctgcgcgac gacccggaca ccgtgcggaa
     3001 gaaggtgcgg cggatggtca ccgacccgca gaaggtgcgc ctcggcgacc ccgggcgccc
     3061 ggagatctgt cccgtcttcg ccctgcaccg gcggttcagc gaggacatcc tcgacgagac
     3121 cgcggccggg tgccgcgccg gcacgctgcc ctgcgtgcag tgcaaggaga atctggcgga
     3181 ccgtctgatc ccggcgctcg ggccgtaccg cgaggcgcgc gagcggctgg agacgacccc
     3241 cggtgtggcc ggcgaggtgc tggcggacgg cgccaagcgc gtccggccgg tggtcgagga
     3301 gacgatcgac gcggtccgca ccgcgatgcg catgaaaggc ctgggggagt gagccgggcc
     3361 gagggggtgc gcgccgagcc gggcgcgttc gccgtcgaga ctccggtctt caccggaccg
     3421 ttccgcgccc tggccgagct gctcctcggc cggaaggtgg acgtctgcga cgtgccgctg
     3481 cgcggcgtgg tcgacgcgtt cgtggccctc gccccggagg ccgggggccg gacgctcgag
     3541 gagcagacct ggttcgtcgt cacgtgcgcg acgctcctcg agatgaaggt cgcgcgcctc
     3601 accccacgcg acatcgagcc cgacgaggaa gacctggtcg gcgggtcgcc cgacctggcc
     3661 tacgcccgaa ggctggagct gtcggcgttc cgggaggctg cccgggaggt ggcccggtgg
     3721 ctggaggatg gcggccgccg cttcggccgc gggtccgggc cgggcg
//
LOCUS       DASPWO010000173         5900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_537208, whole genome shotgun sequence.
ACCESSION   DASPWO010000173 DASPWO010000000
VERSION     DASPWO010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537208"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /locus_tag="VEO00_03880"
     CDS             <1..1830
                     /locus_tag="VEO00_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYU57174.1"
                     /translation="DPDWFWDAVVKDLGLEFFTPYERVMDPSRGIPWTTWFTGGTVNL
                     AHPCVDVWAERTPDAPAVIWEGEDFSVRTVTYRELRAMADRLTHGLASLGVGRGDAVG
                     IFLPMAPETVAATLACAKLGAIYLPIFSGYGADAVATRLADGGAKVLLTADGFLRRGA
                     QVRMKEVADEAADAAPAVERVVVWSRLGRSDLPWGPRDVRWEDLTAGQPDRFETPALD
                     PETPLFVAYTSGTTGRPKGSVHVHGGFLAKIAEEVAYQTDVRPGDVLFWVTDLGWIMG
                     PWEIVGAGALGATVLLFEGAPNHPAPDRLWDMVERHRVTVLGVSPTLIRALIPAGDEH
                     ALKHDLSSLRILGSTGEPWNPEPYDWLLNTVGGGRCPIINLSGGTEVGACFLSPLPIT
                     ELKSCTLRGPALGMAIEVWGPEGTPVGPNEVGELVCTKPWPGMTRGLYKDPDRYLETY
                     WSRWPDVWVHGDWASVDEDGFWFLHGRSDDTMNIAGKRLGPVEVESALVDHPAVVESA
                     AVGVPHAVKGEAIWCFVILRPGAEPTDELADELAAHVADHLGKSFRPSRVVFVDELPK
                     TRSAKILRRALRATVLGDDPGDLSSLENPSALEKARAVTDGTR"
     gene            1999..2502
                     /locus_tag="VEO00_03885"
                     /pseudo
     CDS             1999..2502
                     /locus_tag="VEO00_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            2499..3215
                     /locus_tag="VEO00_03890"
     CDS             2499..3215
                     /locus_tag="VEO00_03890"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HYU57175.1"
                     /translation="MIDHDRVEEEIIDLVLGGMVREERARFAAQLGEHMRTCDLCRTL
                     YAGYSELAGRLALATPPIPPPASLEDRVVAEAAGMGPPVRAPSPRARLRRIALAAAAA
                     GVLVAGSVAATVAVTRPGGGALSGEAVAVLTLPGTRLVQMEVADGGTVAFAYHTAARR
                     SVVVGDGLAALPGGRVYELWLIGAAGPVPGAVFSPDDGRVVQAGDLATTSFDTVALTV
                     EPSGGSDQPTTDPIAQTEVA"
     gene            complement(3212..4606)
                     /locus_tag="VEO00_03895"
     CDS             complement(3212..4606)
                     /locus_tag="VEO00_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYU57176.1"
                     /translation="MRAPRVALVLILLAAGFGISRLVPAASRSPTPQQGRPNLLFILT
                     DDQRFDTLSVMRATRADFDVVFENGFVTTPLCCPSRSSILTGRYASHTGVHGNRDYPR
                     FVPQEAESIGPWLDAMGYYTGFVGKYLNRVPVDKPAPPGWDEFYGLVYDLEGEDGPHG
                     PILRERWMDGDTERQQVVHEPRGTEYTALWTREALRFLHRAADPTVNPDGAPWALFVW
                     TNAPHQPDPDARYRDGPVPGWTPPPSFLEADRSDKPLEVRAGPGRIGDAEVHREVRED
                     QLRQLLSVDDLVARLFAYLDGAGLRERTWGIFSSDNGVFWGEHLLSRKLYAYEEAIRV
                     PLRMAVPGSGPRRIDPIVANVDLAPTLLELAGDRTDHGMDGRSLVGLATGRDPRPVRE
                     ALLIENWANVYWEGVRTPQWKYVHWIATGAEELYDLRADPYELENLAASRHGIVKGLR
                     SKLRRLRQSSPGVG"
     gene            complement(4607..5125)
                     /locus_tag="VEO00_03900"
     CDS             complement(4607..5125)
                     /locus_tag="VEO00_03900"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HYU57177.1"
                     /translation="MTGDGRGPEGRPVSQSEVVLAQQMELEHSNLAGNVHGGVIMRLV
                     DTAAGLAAAKHCGRVAVTVAMDDMSFIEPVFLGDIVTVRAMVNDTGTTSMEVGVRVEA
                     ENVHTGRHAHTSSAYLVFVALDAEGRPTPVPPAVAETEEQRQRQREAKLRREARLARK
                     EAILKVRASRGE"
     gene            complement(5122..>5900)
                     /locus_tag="VEO00_03905"
     CDS             complement(5122..>5900)
                     /locus_tag="VEO00_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU57178.1"
                     /translation="GSGSDAVAMRTLYRRTDGGFRLSGTKRFITGAGVSDAYTVFATR
                     DPALRQEGISAFLLMRDDAGLSFGKPEHKMGIHGSPTREVYLEDVEIAADRLIGEEGE
                     GFTQAMRTLDHSRPSIAAQALGIAQGAFDYAARYALGREQFGRPIARYQGLQFMLADM
                     AMRIEASRLLVYRAAALVDRGDPGTTLAASMAKCYASDTAMAVTTDAVQILGGYGYMA
                     DYPVERMMRDAKITQIYEGTNQIQRVVIARELLKAMEPAG"
BASE COUNT          767 a         2149 c         2169 g          815 t
ORIGIN      
        1 gacccggact ggttctggga cgcggtggtg aaggacctgg gactggagtt cttcaccccc
       61 tacgagcggg tgatggaccc ctcccgcggg atcccctgga cgacctggtt caccggcggc
      121 acggtcaacc tggcccaccc ctgcgtggac gtctgggccg agcggacccc cgacgcgccg
      181 gcggtgatct gggagggcga ggacttctcg gtccgcaccg tcacctaccg cgagctgcgg
      241 gcgatggccg accgcctgac gcacgggctc gcctcgctcg gcgtcggccg gggcgacgcc
      301 gtcgggatct tcctgccgat ggcgccggag accgtggccg cgacgctcgc ctgcgcgaag
      361 ctcggcgcga tctacctgcc gatcttctcc ggctacggcg ccgacgcggt ggccacgcgt
      421 ctggccgacg gcggggcgaa ggtgctgctc accgccgacg ggttccttcg ccgcggcgcg
      481 caggtgcgga tgaaggaggt cgccgacgag gcggccgacg ccgcaccggc cgtcgagcgg
      541 gtcgtggtct ggtcgcgcct cggccgctcc gacttgccgt ggggcccgcg cgacgtccgt
      601 tgggaggacc tgaccgccgg gcagccggac cggttcgaga ccccggcgct cgatccggaa
      661 acgccactgt tcgtcgccta caccagcggg accaccggcc gcccgaaggg ctcggtccac
      721 gtgcacggcg ggttcctggc gaagatcgcc gaggaggtcg cctaccagac ggacgtccgc
      781 cccggcgacg tgttgttctg ggtcaccgac ctcggctgga tcatgggccc ctgggagatc
      841 gtcggcgccg gggcgctggg cgcgaccgtg ctgctgttcg agggcgcgcc gaaccatccc
      901 gcgcccgacc ggctgtggga catggtcgag cgccaccggg tgacggtgct cggcgtctcg
      961 ccgacgctga tccgggcgct catccccgcc ggtgacgagc acgccctgaa gcacgacctg
     1021 tcgagcctgc ggatcctggg ctcgaccggc gagccatgga accccgagcc gtacgactgg
     1081 ctgctgaaca cggtcggcgg cgggcgctgc ccgatcatca acctgtcggg cgggaccgag
     1141 gtcggggcct gcttcctgtc gccgctgccg atcaccgagc tgaagtcctg cacgctgcgc
     1201 gggccggcgc tcgggatggc gatcgaggtc tggggaccgg aaggcacgcc cgtcgggcca
     1261 aacgaggtcg gcgagctggt ctgcaccaag ccgtggccgg ggatgacccg cgggctctac
     1321 aaggacccgg atcgctacct ggagacgtac tggagccggt ggccggacgt ctgggtgcac
     1381 ggcgactggg cgagcgtgga cgaggacggg ttctggttcc tgcacgggcg ctcggacgac
     1441 acgatgaaca tcgccgggaa gaggctcggg ccggtcgagg tcgagagcgc gctcgtcgac
     1501 cacccggcgg tggtcgagtc ggcggccgtc ggcgtcccgc acgcggtgaa gggcgaggcg
     1561 atctggtgct tcgtgatcct gcgccccggc gccgagccga ccgacgagct ggccgacgag
     1621 ctcgccgcgc acgtcgccga tcacctcggc aagtcgttcc ggccgtcgcg ggtcgtgttc
     1681 gtcgacgagc tgccgaagac ccgctcggcg aagatcctgc gccgggccct gcgcgccacg
     1741 gtgctcggcg acgaccccgg cgacctctcc agcctggaga acccctccgc tctggagaag
     1801 gcgcgcgccg tcaccgacgg aacccggtga tccgccggcg ctggcagcgc gaatccgggg
     1861 gagacatgga cccggaggac gccggcgcca gcgtgctgcc gcttcgccgc gcgccggccg
     1921 agggcgggcg agacgcgaac gtcgagcagg agcgcgacgc cgacctggtg cgccggatcg
     1981 ccgccggcga gaagccggct ctaccggcgc tacgccgcgg ccgcctacgg cctggcggtg
     2041 cgcatcgtga ggcaggagtt cctgtcggag gagatcgtgc aggagacgtt cacgtcgctc
     2101 tggcgcggcg cggcggcgta cgacccgggg cggggcagcg tccggacctg gctgctgtcc
     2161 tcggtccacc accgggccgt ggacggcgtc cgccgggagg aggcccagca gcgccgggcg
     2221 atgcaggtcg ccccggatcc cgaccgcgcg gccgacttca ccgacgtcgt cgcggaggcc
     2281 ctcgacctcg cggacgaccg ccgccgcgtc cgcgaggccc tggcaggact tccgaacgag
     2341 cagggcgaag tggttcacct gatgtatttc ggcggcctga cacagaccca ggtcgcgggc
     2401 cggctcggcc ttcccctcgg gaccgtgaag agccgctgcc tcctggcgat gcgccgcctg
     2461 cgcagggccc tcgttccagg ctccccgggg agcgccccgt gatcgaccac gaccgcgtcg
     2521 aggaggagat catcgacctg gtcctcggcg ggatggtccg cgaggagcgg gcgaggttcg
     2581 cggcacagct cggcgagcac atgcggacgt gcgacctgtg ccggacgctc tacgcggggt
     2641 actcggagct ggccggccgc ctcgccctgg ccacgccgcc gatcccgccg ccggcatccc
     2701 tggaggaccg cgtggtggcg gaggccgccg gcatggggcc gcccgtgcgc gcgccgtcgc
     2761 cgcgggcccg gctccgccgg atcgcgctcg cggcggctgc cgccggcgtc ctggtggccg
     2821 ggtccgtcgc ggccaccgtg gcggtgaccc gcccgggcgg cggagcgctc tccggcgaag
     2881 cggtggcggt gctgacgctg cccggcacgc gcctggtcca gatggaggtc gccgacggcg
     2941 gtaccgtcgc gttcgcctac cacacggccg cccgccgctc ggtggtggtc ggcgacggtc
     3001 tagccgcgct ccccggcggg cgcgtgtacg agctgtggct gatcggcgcc gccggccccg
     3061 tgcccggcgc ggtgttctcc cccgacgacg gccgcgtggt ccaggccggc gacctggcca
     3121 cgacgagctt cgacaccgtc gccctgaccg tggaaccgtc cggcggcagc gaccagccca
     3181 cgaccgaccc gatcgcccag acggaggtcg cctagcccac gccgggggat gactgccgca
     3241 ggcggcgcag cttggatcgc aagcccttga cgatgccgtg acgggaggcg gcgaggttct
     3301 ccagctcgta ggggtcggcg cggaggtcgt agagctcttc ggcgccggtg gcgatccagt
     3361 ggacgtactt ccactgcggc gtgcggacgc cttcccagta cacgttcgcc cagttctcga
     3421 tcagcagggc ctcgcgcacc ggacgcgggt cgcggccggt ggccaggccg acgagcgacc
     3481 tgccgtccat gccgtggtcg gttcggtcgc cggccagctc gagcagggtc ggggcgaggt
     3541 cgacgttcgc cacgatcggg tcgatccggc gcgggccgct gccggggacc gccatccgga
     3601 gcggcacgcg gatggcctcc tcgtaggcgt acagcttccg cgacaggagg tgctcccccc
     3661 agaacacgcc gttgtccgag gagaagatcc cccaggtccg ctcgcgcagc ccggccccgt
     3721 cgaggtacgc gaacagccgc gccaccaggt cgtcgacgga gaggagctgc cggagctggt
     3781 cctcgcgaac ctcgcggtgg acctcggcgt ccccgattcg ccccggtccc gcccggacct
     3841 ccaacggctt gtcgctcctg tcggcctcca ggaacgacgg cggtggggtc catcccggta
     3901 ccggcccgtc gcggtagcgc gcgtccgggt ccggctgatg cggagcgttc gtccacacga
     3961 acagcgccca cggcgcgccg tccgggttga ccgtcgggtc ggcggcccgg tgcaggaacc
     4021 gcagggcctc gcgcgtccag agcgccgtgt actccgtccc ccgcggctcg tgcacgacct
     4081 gttgccgctc ggtgtccccg tccatccacc gctcgcgcag gatcggaccg tgcgggccgt
     4141 cctcgccctc caggtcgtag accagcccgt agaactcgtc ccagccgggc ggggcgggct
     4201 tgtccaccgg cacgcggttc aggtacttgc cgacgaaccc ggtgtagtag cccatcgcgt
     4261 cgagccacgg gccgatcgac tcggcctcct gcggcacgaa gcgcgggtag tcgcggttcc
     4321 cgtgcacccc ggtatggctg gcgtaccggc cggtgaggat gctcgagcgc gagggacagc
     4381 agagcggcgt cgtgacgaac ccgttctcga agacgacgtc gaagtcagcg cgggtggccc
     4441 gcatcaccga gagcgtgtcg aaccgctggt cgtccgtgag gatgaacagc aggttcggcc
     4501 gtccctgctg cggagtgggc gaacgtgagg ccgccggaac caggcgagag atcccgaagc
     4561 cggcggccag gaggatcagg acgagcgcga cccgcggcgc cctcacctac tcgccgcgac
     4621 tggcccggac cttgaggatc gcctccttgc gggccagtcg ggcctcgcgg cggagcttcg
     4681 cctcgcgctg gcgctggcgc tgctcctcgg tctcggccac ggccggtggc accggcgtgg
     4741 gccggccctc ggcgtcgagc gccacgaaca ccaggtaggc gctcgacgtg tgggcgtggc
     4801 gcccggtgtg cacgttctcc gcctcgacgc gcacgccgac ctccatcgag gtcgtcccgg
     4861 tgtcgttcac catcgcccgg accgtaacga tgtcgccgag gaagaccggc tcgatgaagg
     4921 acatgtcgtc catcgccacg gtgacggcga cccggccgca gtgcttggcc gccgccaggc
     4981 cggccgcggt gtccaccagg cgcatgatca cgccgccgtg gacgttcccg gccaggttgg
     5041 agtgctccag ctccatctgc tgcgcgagca cgacctcgga ctggctgacc gggcggccct
     5101 ccgggccgcg accatcgcca gtcatcccgc cggctccatc gccttcaaca gctcccgggc
     5161 gatcaccacg cgctggatct ggttcgtccc ctcgtagatc tgggtgatct tcgcgtcgcg
     5221 catcatccgc tcgacggggt agtcggccat gtacccgtac ccgccgagga tctgcacggc
     5281 gtccgtggtg acggccatgg ccgtgtcgga ggcgtagcac ttggccatcg acgccgcgag
     5341 ggtcgtcccc ggatcgcccc ggtcgacgag cgcggccgcc cggtagacca gcaggcgcga
     5401 ggcctcgatg cgcatcgcca tgtcggcgag catgaactgc agtccctggt agcgggcgat
     5461 cggccgcccg aactgctcgc ggccgagcgc gtaccgcgcc gcgtagtcga acgcgccctg
     5521 cgcgatgccg agcgcctgcg ccgcgatgga cggccgggag tggtcgaggg tgcgcatcgc
     5581 ctgggtgaac ccctcgccct cctcgccgat caggcggtcc gcggcgatct ccacgtcctc
     5641 caggtacacc tcgcgcgtcg gcgagccatg gatccccatc ttgtgctccg gcttcccgaa
     5701 cgagaggccg gcgtcgtcgc gcatcagcag gaacgcgctg atcccctcct ggcgcagggc
     5761 cgggtcgcgc gtggcgaaca ccgtgtacgc gtccgagacg cccgcgccgg tgatgaaccg
     5821 cttcgtcccc gacagccgga acccgccgtc ggtgcgccgg tacagggtgc gcatggccac
     5881 cgcgtcggag cccgagccgg
//
LOCUS       DASPWO010000174         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1047677, whole genome shotgun sequence.
ACCESSION   DASPWO010000174 DASPWO010000000
VERSION     DASPWO010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047677"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VEO00_03910"
     CDS             <1..594
                     /locus_tag="VEO00_03910"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HYU57179.1"
                     /translation="PVRFIGNRSSGRMGAAVAGAALARGARVILITGPTALPPPPGAE
                     IVPVETAEEMRAAVLAALPEADAVVMAAAVADFRPAERAGRKLKKEAGPPRIELVPTS
                     DILKELQRVAGRQVLVGFAAETEDLEEAARKKLVDKAVDLVVANEVGTPGTGFSSETN
                     RALIVARDGDDTPLRAYTKAELADVVLDRVVRLMTQR"
     gene            complement(692..2527)
                     /locus_tag="VEO00_03915"
     CDS             complement(692..2527)
                     /locus_tag="VEO00_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HYU57180.1"
                     /translation="MTYELGIDLGTTYTAAAVQRDGRVEVATLGERAASIPSVIYLRE
                     DDEILTGEGANRRGTTEPGRVAREFKRRVGDPTPLILGGSPLSAEALSARLLRWSVGE
                     VARREGSSPDRITVSHPANWGPYKKDRLGEIIRLADLDAPVETITEPEAAAIYYSSQE
                     RVDPGSVIAVYDLGGGTFDAAVLRKTESGFEILGEPEGIDRLGGIDFDEAVFAHVAAS
                     LGGAFEELDPADPAAIAAVARLRADCMDAKEALSNDTEVTIPVMLPNVQTQVRLTRAE
                     FEDMVRPAISETIDAMRRALRSAGVEPEQVTKVLLVGGSSRIPLVARMVSAELGRPVA
                     VDAHPKHAIALGAALHAASVGGTAPDECEVVVDVEPVSAANAVAAGAVAAGALAGAGA
                     AATEAAVADETAAGEATVPIGAAEETAILAEAREGPPPAPTGPPKRSRRGLLIGLVVL
                     VLAAAGGAYFLLFAGDGGGEASATRRPSPTESPSPSPEPTEEKTTVVPPPPPPQTTTT
                     TTATPPSDCTDQTGAGGTATVVATDDPAFTPGCTQMRADQSLVIQNAGADLHSFTMGG
                     FYDQDITPGTSFDTGPIGATLTPGEYLFICKYHPSQTGELRIVEA"
BASE COUNT          335 a         1000 c         1013 g          376 t
ORIGIN      
        1 ccggtgcggt tcatcggcaa ccggtcgtcg gggcggatgg gcgctgcggt ggccggcgcc
       61 gcgctcgccc gcggcgcccg ggtgatcctg atcaccgggc ccacggccct tccaccgccg
      121 cccggcgcgg agatcgtgcc ggtcgagacc gccgaggaga tgcgagccgc ggtgctcgcg
      181 gcgctcccgg aggcggacgc cgtggtgatg gcggcggccg ttgccgactt ccggcccgcc
      241 gagcgcgctg gccgcaagct gaagaaggag gcggggccgc cacggatcga gctggtcccg
      301 acgtccgaca tcctgaagga gctccagcgg gtggccggcc gtcaggtcct ggtcgggttc
      361 gcggccgaga ccgaggacct cgaggaagcg gcccgcaaga agctggtgga caaggcggtg
      421 gacctcgtgg tggcgaacga ggtcggcacg cccgggaccg gcttctcctc ggagacgaac
      481 cgggcgctga tcgtcgcccg cgacggcgac gacactcccc tgcgcgccta cacgaaggcc
      541 gagctggccg acgtcgtcct cgaccgcgtc gttcgattga tgacgcagcg ctagcgccgg
      601 ccgggcggga gagcccgtct gccttggggg ttcgctcgcc ggacgtggtc ggggttccgc
      661 tctccgcagg gcgagctacc cccaaccccc cctacgcttc cacgatcctg agctccccgg
      721 tctggctggg gtggtacttg cagatgaaga gatactcgcc gggcgtcagc gtggcgccga
      781 tcggcccggt gtcgaaggac gtgccgggcg tgatgtcctg gtcgtagaag ccgcccatcg
      841 tgaagctgtg caggtcggcg ccggcgttct ggatcaccag gctctgatcg gcgcgcatct
      901 gcgtgcaccc cggagtgaac gccgggtcgt cggtggcgac caccgttgcc gtgccccccg
      961 cgccggtctg gtcggtgcag tcggagggcg gcgtggcggt cgtcgtcgtc gtggtctgcg
     1021 ggggtggcgg gggcggcaca accgtggtct tctcctcggt cggctccggg ctcggcgagg
     1081 gcgactccgt gggcgagggc cgccgcgtgg ccgacgcttc gccaccgcca tcgccggcga
     1141 acagcaggaa gtaggccccg ccggcggccg ccagcacgag caccacgagg ccgatcagca
     1201 gcccccggcg gctgcgcttc ggaggcccgg tgggcgccgg cggcgggccc tcacgggcct
     1261 ccgccagaat cgctgtctcc tccgcggccc cgatcgggac cgtcgcctcg ccggccgccg
     1321 tctcgtcggc cacggcagcc tccgtcgcgg ccgcgcctgc tccggcgaga gctccggccg
     1381 cgacggcccc ggccgcgacg gcgttggccg ccgacaccgg ttcgacatcg accacgacct
     1441 cgcactcatc gggggcggtg ccgccgacgg acgcggcgtg cagggccgcg ccgagcgcga
     1501 tggcgtgctt ggggtgggcg tcgacggcga ccggtcggcc gagctcggcc gacaccatcc
     1561 gtgcgaccag cgggatgcgc gacgacccgc cgaccagcag caccttggtg acctgctccg
     1621 gctcgactcc ggccgaccgc agggcccggc gcatcgcgtc gatcgtctcg gagatcgccg
     1681 gccggaccat atcctcgaac tcggcccggg tcaggcgcac ctgcgtctgg acattcggga
     1741 gcatgaccgg gatggtcacc tcggtgtcgt tcgagagggc ctccttggca tccatgcagt
     1801 ccgcgcgcag gcgggccacg gcggcgatcg cggcggggtc ggccggatcg agctcctcga
     1861 aggcgccgcc gagcgacgcg gcgacgtggg cgaagacggc ctcgtcgaag tcgatgccgc
     1921 ccaggcggtc gatcccctcc ggctcgccga ggatctcgaa cccggactcg gtcttgcgca
     1981 agaccgcggc gtcgaaggtg ccgccgccga ggtcgtacac cgcgatcacc gagccgggat
     2041 cgacgcgctc ctgcgagctg tagtagatcg cggccgcctc gggctccgtg atcgtctcga
     2101 ccggcgcgtc caggtcggcc agccggatga tctccccgag ccggtccttc ttgtacgggc
     2161 cccagttcgc cgggtgggag acggtgatcc ggtcggggga cgagccctca cgccgggcga
     2221 cctcgccgac gctccagcgg agcaggcgtg ccgagagcgc ctcggccgag agaggcgacc
     2281 cgccgaggat cagcggcgtg gggtccccga cgcggcgctt gaactcgcgc gccacgcgac
     2341 cgggctctgt ggtcccgcgc cggttcgcgc cctcaccggt gaggatctcg tcgtcctctc
     2401 ggaggtagat caccgatggg atcgaggcgg cccgctcgcc gagcgtggcc acctccaccc
     2461 ggccgtcgcg ctgcaccgcc gcggccgtgt aggtggtgcc gagatcgatc cccagttcgt
     2521 aggtcacggc gctccttgac cgatcggccg gtcggcggtg gccgggccgg agccctccgg
     2581 catcccccgg ctcggcctca cggtgccgcg catcctactc gccggaccgg cggcctgggc
     2641 agggccttac agccccagcc agtcgccgac ctcctccgcc gcgtcgccga cggcctcgat
     2701 gccctcgtcg aggccctcag atcg
//
LOCUS       DASPWO010000175         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_367799, whole genome shotgun sequence.
ACCESSION   DASPWO010000175 DASPWO010000000
VERSION     DASPWO010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367799"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VEO00_03920"
     CDS             complement(<1..384)
                     /locus_tag="VEO00_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HYU57181.1"
                     /translation="MTFATARVRRSTALLLAAGLLAGLLGAGGAGAATSTTTYTVNTA
                     LDSADGACTATNCSLREAILAANRRFGSSDVVKFKIPGALPATIHLGTSLPPVRDPVR
                     IDGTTQPGYAGSPVIVVDGAGLGTAA"
     gene            complement(418..1167)
                     /locus_tag="VEO00_03925"
     CDS             complement(418..1167)
                     /locus_tag="VEO00_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYU57182.1"
                     /translation="MRISDGSRADGNRQPALEVHKALADDTRFRLYRYLNLTGRPTSV
                     QELARRLSLHPNTLRPHLRRLEEVGLVGREIRKGASVGRPQTLYAAVEQAPAESDHRL
                     LAEILAGTIGSKRSLDAVERQAEEWGAYLVGLGGPKPGVRLPAGRNLAILRDAMARAG
                     FDPRFRRTGPAAVEVTLRDCPFRDLVDEHRDLVCTVHRGLIAGMLGGLKPRMRLRELR
                     PFVERGICRVQAATTAPRPDPDRLTKRPAGL"
     gene            1216..>2065
                     /locus_tag="VEO00_03930"
     CDS             1216..>2065
                     /locus_tag="VEO00_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HYU57183.1"
                     /translation="MSAATAPTEEQVRQALRQVLDPEIGRPIEDLGMLQGIAVEDGVV
                     TVDVLLTVPGCPLKDEINRRVTDAVSPLEGVREVRVLLGQMTPDQRESLVTNLRGGRP
                     EAQTFFTDGKTQVVAVASGKGGVGKSSVTVNVAAALAAEGYRVGVLDADVWGFSVPRM
                     MGISGQPVAFNNMILPLEAHGVKIISMGFFVTEETPVIWRGPMLHKAVQQFLGDVYWG
                     DLDFLLADLPPGTGDVSISMASFLPGANMLVVTTPQEAARKVAERAGKMAGQARLRVV
                     GVIENMS"
BASE COUNT          289 a          720 c          783 g          273 t
ORIGIN      
        1 ggccgcggtc cccaacccgg ccccgtccac cacgatcacc gggctcccgg catagccggg
       61 ctgtgtggtc ccgtcgatcc ggacgggatc tcgcaccggc gggagcgacg tccccaggtg
      121 gatcgtcgcc ggaagcgcgc ccgggatctt gaacttcacc acgtcggagg acccgaaccg
      181 ccggttggcg gcgaggatcg cctcgcgcag gctgcagttc gtcgccgtgc aggctccgtc
      241 ggcagagtcg agggccgtgt tcacggtgta cgtggtggtg ctggtcgccg cgcccgcacc
      301 gccggcgccg agcaggccgg cgaggagccc ggccgcgagg agcagcgcgg tggagcggcg
      361 aacgcgggcg gtggcgaagg tcatatcggc gatgggggga tgggggatgc cgcgacgcta
      421 gaggccggcc gggcgcttcg tcaaccggtc cgggtcgggc cggggagcgg tggtggcggc
      481 ctggacgcgg cagatcccgc gctcgacgaa cggccgcagc tcgcgcagcc gcatccgcgg
      541 cttcaggccg ccgagcatgc cggcgatcag gccgcggtgc accgtgcaga ccaggtcgcg
      601 gtgctcgtcc accaggtccc ggaacggaca gtcccgcagg gtgacctcca cggcggccgg
      661 cccggttcgc cggaaccgcg ggtcgaagcc ggcccgggcc atcgcgtcgc gcaggatcgc
      721 caggttgcgt ccggccggga gccgcacccc cggcttcggg ccaccgaggc cgacgaggta
      781 cgcgccccac tcctccgcct ggcgctcgac ggcgtcgagc gaccgcttcg agccgatcgt
      841 cccggccagg atctcggcca gcaggcggtg gtcgctctcc gccggcgcct gctcgacggc
      901 cgcgtagagg gtctgcgggc ggccgaccga ggcgcccttg cggatctcgc ggccgacgag
      961 cccgacctcc tccagccggc gaaggtgcgg gcggagcgtg ttcgggtgca gggacaggcg
     1021 ccgcgccagc tcctggacgg aggtcggacg cccggtcagg ttgaggtagc ggtagagccg
     1081 gaaccgcgtg tcgtcggcca gcgccttgtg cacctcgagc gccggctgac ggttcccgtc
     1141 ggcccggcta ccatcggaga tacgcaccgt gccaaacagg ataatccccg gacctccgac
     1201 gaccacgagg cgaccatgag cgcagcgacc gcaccgaccg aggagcaggt ccgccaggcc
     1261 ctgcgccagg tgctcgaccc cgagatcggc cggccgatcg aggacctcgg gatgctgcag
     1321 ggcatcgcgg tcgaggacgg cgtggtcacg gtggacgtcc tgctgacggt gccgggatgc
     1381 ccgctgaagg acgagatcaa ccgccgggtc accgatgcgg tctcgccgct cgagggcgtc
     1441 cgcgaggtgc gcgtgctgct cggccagatg acgccggacc agcgcgagtc gctggtgacc
     1501 aacctgcgtg gcggccggcc cgaggcccag accttcttca ccgacgggaa gacgcaggtg
     1561 gtggccgtcg cctccgggaa gggcggcgtc ggcaagtcga gcgtcaccgt gaacgtggcc
     1621 gcggcgctcg ccgccgaggg ctaccgggtc ggcgtgctcg acgccgacgt gtggggcttc
     1681 tccgtgccac ggatgatggg gatctccggg cagccggtgg ccttcaacaa catgatcctg
     1741 ccgctcgagg cccacggcgt gaagatcatc tcgatggggt tcttcgtcac cgaggagacc
     1801 ccggtgatct ggcgcggccc gatgctgcac aaggcggtgc agcagttcct cggcgacgtc
     1861 tactggggcg acctcgactt cctgctggcc gacctgccac cggggaccgg cgacgtctcg
     1921 atctcgatgg cctcgttcct ccccggcgcg aacatgctcg tggtcaccac gccgcaggaa
     1981 gccgcgcgca aggtggcgga gcgcgccggg aagatggccg ggcaggcgcg cctgcgcgtg
     2041 gtcggcgtga tcgagaacat gtcgt
//
LOCUS       DASPWO010000176         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1048079, whole genome shotgun sequence.
ACCESSION   DASPWO010000176 DASPWO010000000
VERSION     DASPWO010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048079"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..629
                     /locus_tag="VEO00_03935"
     CDS             117..629
                     /locus_tag="VEO00_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57184.1"
                     /translation="MDVAARIQQLEELITEAKSMPLSSSALVNREEVLELIQGLRQAM
                     PEEIKQARWVVKDREELLLKARRDAEALIEKAHQEQGRLVARESVVMKSQEEAERILS
                     EARDEARQIRLEAEDYVDAKLAAFEIALTKTREELDRTLAQVQRGRDKLRGTTRAQEQ
                     LAREEGGEGQ"
     gene            626..1168
                     /locus_tag="VEO00_03940"
     CDS             626..1168
                     /locus_tag="VEO00_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HYU57185.1"
                     /translation="MISHVDVRDLIDHPGAAREVHLAEPVGAVETPVAEVPSDAPVVV
                     DVLLESVVEGILVSGRVAGTVTFRCARCLTEFERHVAPEVRELFTPDDEEEDEDRYPL
                     GTEGYLDLVPMVRDAIVLDLPFSPLCRPDCRGLCPRCGGDRNLGECTCGPEADPRWAG
                     LAELERLLQDEGNHETRSDT"
     gene            1199..1375
                     /gene="rpmF"
                     /locus_tag="VEO00_03945"
     CDS             1199..1375
                     /gene="rpmF"
                     /locus_tag="VEO00_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HYU57186.1"
                     /translation="MAVPKRKKSRANTRARRSTWKAAPPTYAECPQCHQPKVPHRVCA
                     NCGYYAGKPVLEVE"
     gene            1452..2129
                     /gene="rnc"
                     /locus_tag="VEO00_03950"
     CDS             1452..2129
                     /gene="rnc"
                     /locus_tag="VEO00_03950"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HYU57187.1"
                     /translation="MRVRFANRDILQHALTHRSYAFETGVAPTNERLEFLGDAVLGLV
                     VTDLAYRQFPHHSEGDLAKLRAATVNMTTLAEVARALGLGEHVLLGRGEELSGGRDKA
                     SILADALEAVLGAVYIDRGLRPARRLIERLFWPRMAAYARGEGDRDFKTGLQELAAQD
                     LGDAPRYQITERGPDHAKEFTAIVFLSGKPYGRGRGRSKKEAEQEAAREAFGVLTGST
                     DTLAKEG"
     gene            2129..2971
                     /gene="mutM"
                     /locus_tag="VEO00_03955"
     CDS             2129..2971
                     /gene="mutM"
                     /locus_tag="VEO00_03955"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HYU57188.1"
                     /translation="MELPEVEVMRRDLEKDVVGKRIKSVDVKPHKNAMRAIRRHGRRK
                     EFADRLAAKKILKVDRKGKYILLMLEGEDVLVIHFGMSGQLLRGTKRNPLPLHTHVVI
                     DFQQGGDLRFVDPRTFGELFVATRDELGKIKELAHVAIDPLEDTFTWPQFSEVLVQRK
                     AKLKPLLMDQKFVSGLGNIYSDEVLWAAGLRHERMSDALSSQEVRRLYRGMQEVLQDS
                     IRYRGTTLDDEAYLDLYGKPGEYQNELRVYGRKGRPCYRCRTPIATVLISGRTSYYCP
                     QCQS"
     gene            2959..>3170
                     /locus_tag="VEO00_03960"
     CDS             2959..>3170
                     /locus_tag="VEO00_03960"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HYU57189.1"
                     /translation="MPIVTATKRVRVFASGRVQGVGFRYVTAQRARAMGVGGFVRNLP
                     DGRVEAAFEGEDEAVDALVEFVRGGPG"
BASE COUNT          505 a         1065 c         1174 g          426 t
ORIGIN      
        1 tggtcgtacc tgtcgtcctc gctgatgaag gaggtcgtcc ggttcggggg gtccgtggac
       61 ggcctggtcc ccgacttcgt gcgcgaccgc ctgaccgaga agctgggggg gtcctagtgg
      121 acgtcgccgc ccggatccag cagctcgagg agctgatcac cgaggccaag tcgatgccgc
      181 tgtcgtcctc ggccctggtc aaccgggagg aggtcctgga gctgatccag gggctgcgcc
      241 aggccatgcc cgaggagatc aagcaggcgc gctgggtggt gaaggaccgc gaggagctgc
      301 tgctgaaggc gcgccgcgac gccgaagcgc tgatcgagaa ggcgcaccag gagcagggcc
      361 gcctcgtggc ccgcgagtcg gtggtgatga agtcccagga ggaggccgag cggatcctgt
      421 ccgaggcccg cgacgaggcc cggcagatcc gcctggaggc cgaggactac gtggacgcca
      481 agctcgcggc gttcgagatc gcgctgacca agacgcgcga ggagctggat cggacgctgg
      541 cgcaggtgca gcggggccgc gacaagctgc gcggcactac ccgggcccag gagcaactgg
      601 cgcgcgagga ggggggcgag gggcagtgat ctcccacgtc gacgtgcggg acctgatcga
      661 ccaccccggc gccgcgcgcg aggtccacct cgccgagccg gtcggggccg tcgagacgcc
      721 ggtggcagag gtgccctcgg acgcgccggt cgtcgtggac gtcctgctcg agagcgtggt
      781 cgagggcatc ctcgtgtcgg gccgggtcgc cgggaccgtg acgttccgct gcgcgcgatg
      841 cctgaccgag ttcgagcgcc acgtcgcacc cgaggtgcgc gagctgttca ctccggacga
      901 cgaggaggag gacgaggacc ggtatccgct cggcactgag ggctacctcg acctggtgcc
      961 gatggtccgc gacgccatcg tcctcgactt gccgttctcc ccactgtgcc gcccggactg
     1021 ccggggcctg tgcccgcgat gcggtggcga ccgcaacctc ggggaatgca cctgcggacc
     1081 cgaggccgac ccgcgctggg ccggcctcgc ggagctcgag cggctcctac aggacgaggg
     1141 gaaccacgag acgcgatccg acacctgatc gagagagaag agagaggaag acccacccgt
     1201 ggccgttccg aagcgcaaga agtcccgagc caacacccgg gcccgccgtt cgacctggaa
     1261 ggcggcgcca ccgacgtacg cggagtgccc gcagtgccac cagccgaagg tcccgcaccg
     1321 cgtgtgcgcg aactgcggct actacgcggg caagccggtc ctcgaggtcg agtaggggcg
     1381 ccggagggcc tgcgatcgcg accggatcgg aacggcgcac ggttcctgcc cgggcggcgc
     1441 ttgagcgcgc cctgcgcgtc cgcttcgcca accgggacat cctgcagcac gcgctgaccc
     1501 atcgctccta cgccttcgag accggggtgg cgccgacgaa cgagcgcctg gagttcctcg
     1561 gcgacgcggt cctcgggctg gtggtcaccg acctcgccta ccggcagttc ccgcatcata
     1621 gtgagggcga cctggccaag ctccgcgcgg cgacggtgaa catgacgacg ctggcggagg
     1681 tggcccgcgc gctcggcctc ggcgagcacg tactgctcgg gcggggcgag gagctgtccg
     1741 gcggccgcga caaggcgagc atcctggccg acgcgctgga ggccgtgctc ggcgccgtat
     1801 acatcgaccg cggcctgcgc cccgctcgcc ggctgatcga gcggctcttc tggccgcgca
     1861 tggccgccta cgcgcgcggc gagggtgacc gcgacttcaa gaccggcctg caggagctgg
     1921 cggcgcagga cctcggcgac gcgccgcgct accagatcac cgagcgcggc cccgaccacg
     1981 cgaaggagtt cacggcgatc gtgttcctgt ccgggaagcc gtacgggcgc ggacgcggac
     2041 gttcgaagaa ggaggccgag caggaagccg cgcgtgaggc gttcggtgtg ctcacgggct
     2101 cgaccgacac cctggccaag gagggctgat ggagctcccc gaggtcgagg tgatgcgccg
     2161 ggacctggag aaggacgtcg tcggcaagcg catcaagtcc gtcgacgtca agccccacaa
     2221 gaacgccatg cgcgccatcc gccgccacgg ccggcgcaag gagttcgccg accgcctggc
     2281 cgccaagaag atcctgaagg tcgaccgcaa gggcaagtac atcctgctga tgctcgaggg
     2341 cgaggacgtc ctggtgatcc acttcgggat gagcgggcag ctgctgcgcg ggaccaagcg
     2401 caacccactg ccgctgcaca cccacgtggt gatcgatttc cagcagggcg gcgacctgcg
     2461 cttcgtcgac ccgcgtacgt tcggcgagct gttcgtcgcc acgcgcgacg agctcggcaa
     2521 gatcaaggag ctggcccacg tggcgatcga cccgttggag gacacgttca cctggccgca
     2581 gttctccgag gtgctggtgc agcgcaaggc caagctgaag ccgctgttga tggaccagaa
     2641 gttcgtctcg gggctcggca acatctactc cgacgaggtg ctgtgggcgg cgggactgcg
     2701 ccacgagcgc atgtccgacg cgctgtccag ccaggaggta cgccgcctgt accgcgggat
     2761 gcaggaggtg ctgcaggact cgatccgcta tcgcgggacc acgctcgacg acgaggcgta
     2821 cctcgacctg tacggcaagc cgggcgagta ccagaacgag ctgcgcgtct acggccgcaa
     2881 gggccgcccc tgctaccgct gccggacccc gatcgccacg gtgctgatct ccggccgcac
     2941 ctcgtactac tgcccgcagt gccaatcgtg accgccacga agcgggtgcg ggtgtttgcg
     3001 tcggggcggg tgcagggcgt ggggttccgc tacgtcacgg cgcaacgcgc gcgggccatg
     3061 ggcgtcggcg gcttcgtgcg caacctcccc gacggacggg tcgaggccgc cttcgagggg
     3121 gaggacgagg cggtggacgc cctcgtcgag ttcgttcggg gcggtccggg
//
LOCUS       DASPWO010000177         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_449326, whole genome shotgun sequence.
ACCESSION   DASPWO010000177 DASPWO010000000
VERSION     DASPWO010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449326"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1641)
                     /locus_tag="VEO00_03965"
                     /pseudo
     CDS             complement(1..1641)
                     /locus_tag="VEO00_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334922.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
     gene            complement(1727..2029)
                     /locus_tag="VEO00_03970"
     CDS             complement(1727..2029)
                     /locus_tag="VEO00_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57190.1"
                     /translation="MRFEFHDNGTRVGSAQWEGPGQIHLEVEDEAERTYLTEYFAGEM
                     TYLCTPFDEDEDAFQIRRRDWTPWEFERACVALARTRGYRVHMTPSEPVEARPQAG"
     gene            complement(2283..>2907)
                     /locus_tag="VEO00_03975"
     CDS             complement(2283..>2907)
                     /locus_tag="VEO00_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57191.1"
                     /translation="RGDIARLYDADPFDLYATSEAGVVAWQCARRDAYHVNADAVVVE
                     ALGRDGAPVPVGETGEVALTVLWNRTTPFVRYRVGDLAALRPGFCGCGVALPLLSRPE
                     GRTNDWVVRVDGGRVSPFRLTLGVLGPDLIAATRRYRIVQRAVNDFLLEVVWRDAPVP
                     DLAERLGTVYARLLGGPVAVVVRTVDRLVVPPGSKYRLVESRVSRSA"
BASE COUNT          397 a         1072 c         1016 g          422 t
ORIGIN      
        1 ggccggccgg gaccatcggg aagacgcctt cgtgggcgtc gacgcggaag tccaccacca
       61 ccggcacggc gttctcggcc agcgccttct ccagggtggc gtcgacgtcc tccggccgtt
      121 cgcagcgcag cccgagggcg ccgtacgcct ccgccagctt gacgtagtcg ggcacgtcga
      181 agccgaggtg cacctcggag tagcgctcgt cgtagaacag ctcctgccac tggcgcacca
      241 tgccgaggtg gccgttgttg aggatcagca ccttgatggg gatccgctcg gtggtgcagg
      301 tggccagctc ctgggcggtc atctggaagc agccgtcgcc gtcgatcgcc accaccagct
      361 cgtcgggccg gccgaccttg gcgcccatcg ccgccggcac cgcgaagccc atcgtcccca
      421 gcccgccgga gttcacgaac tggcgcgggc gcttgaagcg gtagaactgc gacgtccaca
      481 tctggtgttg gccgaccccg ctgacgatcg tcgcgtcgcc ccggtcgccg cgtggatccg
      541 ctccacgacg aactgcgcct tgatcggccc gtcgtcctcc tgcgcgtagc gcagggggta
      601 gcgcgcctgc cagtcgtgca gggtggcgag ccagcgggac cggtcgggcg cgccgcgttc
      661 ggcctgcgcc ttcgccagct cgcgggccag cgcgcccgtg acgtctctgg cgtcgccgac
      721 gatcggcacg tcggccgcac ggttcttccc gatctccgcc gggtcgatgt cgacgtggat
      781 caccttcgcg ttcggggcga acgtctgcgg gctgccggtg acccggtcgt cgaagcgggt
      841 cccgagggcg atgagcaggt cggccttctg caacgcggtg accgccgtgt agttgccgtg
      901 catccccggc atcccgagcc ccagctcgtg gtcgtcgggg aacgccccgc gcgccatcag
      961 cgtggtgacc accggcaggc gcccggcctc ggccagcgcg cgcagctccc cggacgcgtt
     1021 cgccttgatc agtccgccgc cggcgtagat caccggccgc tcggcggcga ggatcagcct
     1081 cacggcctcg cggacctgct tcgggtttcc cttcgtcgtc ggccggtagc cgggcagatc
     1141 gatctcctcc ggccactccc agccggcctc ggacagcagg acgtccttcg ggaggtcgat
     1201 cagcaccggg cccggccggc cggtcgtcgc gatgtggaac gcctcgcgca cgacgcgcgg
     1261 gatgtcacca gcgtcggtga ccagccagtt gtgcttggtc accggcagcg tgatcccggt
     1321 gatgtcggcc tcctggaacg cgtcgttgcc gaccacggcc gtcgggacct ggccggtgat
     1381 cgccacgacc ggggccgagt ccatcagcgc gtcggcgagc gcggtgacca ggttgcagcc
     1441 ccccgggccg gacgtggtca tgcagacgcc cgggcggccc gacgcgtgcg cgtagccctc
     1501 ggcggcgtgg ccggcaccct gctcgtggcg catcagcacg tggcgcaccg gcgaatcgag
     1561 gatcgggtcg tacgccggga ggatcccgcc cccggggatg ccgaagacga cgtcgacgcc
     1621 ctcggcctcc agagagcgca tcaggctgat agcgcccgtg acgcgcgtgc ccctgcgcga
     1681 ccaggccgtg gtggtcccgg cgctcctctt cccggcggcc actcgctcac cccgcctgcg
     1741 gacgcgcctc gaccggctcg gacggggtca tgtgcacgcg gtagccgcgg gtgcgggcca
     1801 gcgcgacgca cgcccgctcg aactcccacg gcgtccagtc gcgacggcgg atctggaacg
     1861 cgtcctcgtc ctcgtcgaac ggggtacaca ggtacgtcat ctcgccggcg aagtactcgg
     1921 tcaggtacgt ccgctcggct tcgtcctcga cctccaggtg aatctgcccg ggaccctccc
     1981 actgcgcgga cccgaccctg gtgccattgt cgtggaactc gaaccgcatg gcggtgctcc
     2041 tctcggtgcg tggaacggcg ctgttccggt tggctcgtgg tttcggagcg ccggtacccc
     2101 cgggggacgg cggtgtcccg gaaatgagaa acccctccgg aggaggggtc ttcggcgcgg
     2161 tcagggcgtg cgtcgccccg ccccgcgcct aggtaatacg tacgaggttc agtcggccgc
     2221 gaacgtcgaa ccgagtgacc atgtggaacg gggagtacac gccatcgatc agattcgtca
     2281 agtcacgcgc tcctcgagac ccgcgattcg accaagcggt acttgctgcc gggtggcacg
     2341 accaggcggt ccaccgtgcg gaccaccacc gccaccggtc caccgagcag ccgtgcgtag
     2401 accgttccga ggcgttccgc caggtcggga acgggcgcgt cgcgccacac cacctcgagc
     2461 aggaagtcgt tcaccgcccg ctgaacgatc cggtagcggc gcgtggccgc gatcaggtcc
     2521 gggcccagga cgccgagggt gagccggaac ggggagacgc ggccgccgtc gacgcggacg
     2581 acccagtcgt tcgtgcggcc ctccggccgc gacagcagcg ggagcgccac gccgcacccg
     2641 cagaaccccg gacggagcgc cgccaggtcg ccgacacggt accggacgaa cggcgtggtc
     2701 cggttccaga gcaccgtgag cgcgacctca ccggtctcgc ccaccgggac gggcgcgccg
     2761 tcccggccaa gggcctccac caccaccgcg tcggcgttca cgtgatacgc gtcgcggcgc
     2821 gcgcactgcc acgccacgac cccggcctcg ctcgttgcgt acaggtcgaa cgggtcggcg
     2881 tcgtagagcc gcgcgatgtc cccgcgc
//
LOCUS       DASPWO010000178         5620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1128428, whole genome shotgun sequence.
ACCESSION   DASPWO010000178 DASPWO010000000
VERSION     DASPWO010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128428"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VEO00_03980"
     CDS             <1..210
                     /locus_tag="VEO00_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HYU57192.1"
                     /translation="IEAVLRGLQEDRELPSTQAFQPIRAAVTGALVSPPLWESLALLG
                     RDRTLARLHAAADRAEGRSRSAGGP"
     gene            259..1023
                     /locus_tag="VEO00_03985"
     CDS             259..1023
                     /locus_tag="VEO00_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57193.1"
                     /translation="MAKTTKKRGPTPPRSVREQARRKPGQIAPTRDRHRQRRRANIIT
                     AAIIFLLLGGAIAVGVIANLGPDELPGLQISRPVPPETWQPETTSLARRIQIQDIPPP
                     GSEAFHIHTHLDLFVDGVEMPVPNGVGVGTTVFAGLHTHNELGTIHVESDTPFTATLG
                     DFLDIWGVRFIATCLGGYCADGDHPLEVYVNGKRFTGDDPRGIVLKDLDEIALVYGEA
                     PDQIPEDFDFTKDPSVAPQGTPIPTPTASAGATPTG"
     gene            1034..1744
                     /locus_tag="VEO00_03990"
     CDS             1034..1744
                     /locus_tag="VEO00_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57194.1"
                     /translation="MKGCPYCGGTIQDAAVKCRWCGRMLVPPTPPTEAGPTFTHSGVR
                     YVLGYGHDLIGIWDREAPAEPAERYPRSADGWVVAWRRFAALEPGAEAVAPAYRAHAA
                     PGARQPGPSVTAAAVMLIVIGALYAISGAVFLAVPEATGDIGATSGEVRAGGTFVLAF
                     GILEIVAGIMVVRRARAGRTLGIVLASIAAVFALGTVGGGGVMAGLVLGAGSVFVIVA
                     LARAGAAFPVRGGPAPPP"
     gene            complement(1741..2472)
                     /locus_tag="VEO00_03995"
     CDS             complement(1741..2472)
                     /locus_tag="VEO00_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57195.1"
                     /translation="MIQVRKVLVGILLLVLGFGALPVGLAVGGPSRFAVIGLSAGCIL
                     AGGLLLVLGIRSITRLATTAASPTGPVLPSQIAAAEPEITLPAGVPEASRAAVEQALE
                     MMRSMGVNIDPKRFEHATFVDTGGTGGAFPMVAGGTGFAVADAALLSAGLDGTATVSS
                     VVPAGVVVNGMAVAALTLSVQAPGRPPYAVQHQEMLPADRLATLAPGVTLLVKIDPVD
                     PNRVAVVWPGRPSPPAGPATGGLRI"
     gene            complement(2658..2957)
                     /locus_tag="VEO00_04000"
     CDS             complement(2658..2957)
                     /locus_tag="VEO00_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57196.1"
                     /translation="MDDQAWSLLIGAGFVDSWVEADESPAFTAGQPDDLDCTLPSTLD
                     NMVDFVLHDADGTVDAVRHSGDIVGEDPGDCTTGTPPLWPSDHAGVVVALHLPEA"
     gene            3074..3289
                     /locus_tag="VEO00_04005"
     CDS             3074..3289
                     /locus_tag="VEO00_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57197.1"
                     /translation="MIGVGIFAIAAVGDMIWHTVLGLAPLLSIVFFAAAIAFFFGFAS
                     PFNRSGTAFDGACSPPGWRRRGSRMLG"
     gene            3486..5620
                     /locus_tag="VEO00_04010"
                     /pseudo
     CDS             3486..5620
                     /locus_tag="VEO00_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          822 a         2023 c         1956 g          819 t
ORIGIN      
        1 atcgaggcgg tcctgcgagg cctgcaggag gaccgggaac tgccgtccac gcaggccttc
       61 cagccgatcc gtgcggcggt gaccggcgct ctcgtctcgc cgccgctgtg ggaatcgctg
      121 gcgctgctcg ggcgggaccg cacactggcg cgcctgcacg cggccgccga ccgcgccgag
      181 ggccgttctc ggagcgccgg cggcccctga cgcaccacgc cgtcaaaccc gcgagcgtct
      241 ggtacggtcg ggtgcccgat ggccaagacc accaagaagc gggggcccac gcctccccgc
      301 agcgtccgcg agcaggcccg ccgcaagccg ggacagatcg cgccgacgcg cgaccgccac
      361 aggcagcggc ggcgcgccaa catcatcacc gcagccatca tcttcctgct gctcgggggc
      421 gcgatcgcgg tcggcgtgat cgccaacctg gggccggacg agctgcccgg acttcagatc
      481 agccgcccgg tccccccgga gacctggcag cccgagacga ccagcctggc ccggcggatc
      541 cagatccagg acatcccgcc ccccgggtcc gaggccttcc acatccacac gcacctcgac
      601 ctcttcgtgg acggcgtcga gatgccggtt cccaacgggg tcggggtggg gaccacggtc
      661 ttcgccgggc tgcacaccca caacgagctg ggcaccatcc acgtggagtc cgacaccccg
      721 ttcaccgcga cgctcgggga tttcctcgac atctggggcg tgcgcttcat cgcgacgtgc
      781 ctcggcggct actgcgccga cggagaccac ccgctcgagg tgtacgtgaa cgggaagcgg
      841 ttcaccggcg acgatccccg cgggatcgtg ctgaaggacc tcgacgagat cgcgttggtc
      901 tacggggagg cgcccgacca gatccccgag gacttcgact tcacgaagga cccatcggtc
      961 gctccgcagg ggaccccgat cccgacgccg acggcgtcgg ccggcgccac cccgaccggc
     1021 tgacgcccca cccgtgaagg gctgcccata ctgcggcggc acgatccagg acgcggcggt
     1081 caagtgccgc tggtgcgggc ggatgctggt gccgcccacc ccgcccaccg aggcgggacc
     1141 gaccttcacg cactccggcg tccgctacgt cctcggctac ggccacgacc tcatcggcat
     1201 ctgggaccgg gaggcccccg ccgagccggc cgagcgctat ccgcggagcg ccgacggctg
     1261 ggtggtcgcc tggcgtcgat tcgccgccct cgagccggga gccgaggcgg ttgcgcccgc
     1321 ataccgggcg cacgcggccc cgggggcgag gcagcccggg ccctcggtca ccgcggctgc
     1381 ggtgatgctg atcgtgatcg gagctctgta tgcgatctcc ggagcggtgt tcctggcggt
     1441 tcccgaggcg accggcgaca tcggcgccac gagcggcgag gtcagggccg gcgggacgtt
     1501 cgtgctcgcc ttcgggatcc tggagatcgt ggccggcatc atggtcgtgc gccgggcccg
     1561 ggccggccga acgctcggca tcgtcctggc gtccatcgcg gccgtcttcg ccctgggcac
     1621 ggtggggggc ggcggggtga tggccggcct ggtgctgggc gccgggagcg tgttcgtgat
     1681 cgtggcgctc gcgcgggcgg gcgccgcctt cccggtgcgc ggtggcccgg ccccgcctcc
     1741 ctagatccgg aggccgccgg tggcgggccc ggcgggcggt gacggccgtc ccggccagac
     1801 cacggccacc cggttcgggt cgaccggatc gatcttcacc aacagcgtga cgcccggcgc
     1861 gagcgtcgcg agccggtcgg ctggaagcat ctcctggtgc tgtaccgcgt agggcggcct
     1921 ccccggagcc tggacggaga gcgtcagcgc ggccacggcc atgccgttca ccacgacgcc
     1981 ggcgggcacg accgagctca cggtggcggt cccgtccagc ccggcggaga gcagggcggc
     2041 gtccgcgacc gcgaaacctg ttccgccggc gaccatcggg aacgctccgc cggtgccccc
     2101 ggtgtcgacg aacgtggcgt gctcgaagcg cttcgggtcg atgttcactc ccatcgagcg
     2161 catcatctcg agcgcctgct cgaccgccgc gcgcgacgcc tcggggacgc ccgccggcag
     2221 cgtgatctcc ggctccgcgg cggcgatctg cgacgggagc acggggcccg tcggtgaggc
     2281 cgccgtcgtc gccagccgcg tgatcgagcg gatgccgagg acgagcagga gtcctccggc
     2341 gaggatgcag ccggcgctca ggccgatcac cgcgaaccgc gacgggcccc cgacggcgag
     2401 ccccaccggc agcgccccga accccaggac gagcagcagg atccccacga ggaccttccg
     2461 cacctggatc accgcgccag cgtagcgcag ggctgcctcg cgcctcgacg gccgggggag
     2521 gcccgcgaac tcccgccgag aacgtcgagg gcatgtggag gaggcgccgc actcttctcg
     2581 ccaccgttct tggccggcac aggagaaggc ccccgagacc gggattggcc tcgggggcca
     2641 tcatcctgtc gcggtctcta cgcctccggg aggtgcagcg ccacgaccac gccggcatgg
     2701 tcggagggcc acagcggcgg cgtcccggtc gtgcagtcgc cgggatcctc cccgacgatg
     2761 tcgccggagt ggcggacggc gtcgacggtg ccgtccgcgt cgtggaggac gaagtcgacc
     2821 atgttgtcga gggtcgaggg caacgtgcag tccaggtcgt cgggctgccc ggccgtgaag
     2881 gcggggctct cgtccgcctc cacccagctg tccacgaacc cggcaccgat gagcaggctc
     2941 caggcctgat cgtccatcga gcgaaggccc tgcgggtaga gtttgatcct ggcattcccc
     3001 ggcttctacc tgggctgagg gagaagcgtg ccgttcctgg cgtgcgtcgg cgcctcgggg
     3061 ctacggactg ggagtgatcg gtgtcgggat cttcgccatc gccgcggtgg gagacatgat
     3121 ctggcacacc gtgctcggcc tcgcccctct gctgtccatc gtcttcttcg cggcagcgat
     3181 cgcgttcttc ttcggattcg cctcgccctt caatcggagt gggaccgcct tcgacggcgc
     3241 ctgttcaccg cccggctgga ggcggcgggg atccaggatg ctgggctgat cttcaggtga
     3301 gctacctgat gctgccgcca ctccggccgc agctcgatca aaaggaccgg accgggccct
     3361 gatcccctcg cgaaggctca catggcgagg gctccactcc gcatttgtcc ccgttcgagt
     3421 aaccagcgag gattctgagc aggttcgggg cccgcctact ggaagggcgg ggaatgctga
     3481 gaaacatgag gttcgtcctc gtggcactgg tagcacttgg ctcggtgccg ggcttctcgc
     3541 tcgccgcccc tgccgacgtg gcggcaacgc cattggcagg tttggaggct ccggtcaagg
     3601 tcgtgcgcga cgcgaacggc gtgccgcatg tgtacgcgcg gagcgagcac gacgcgtatt
     3661 tcctggttgg ctatctccac gcccaggatc gcctgttcca gatggaccag agccggcggc
     3721 aggcgagcgg tacgctcgcc gagctcctcg gccccaacgc gctgccgagc gacgcgttcc
     3781 tgcggacgat cggcttgcgt cgggcggccg cgcgctcgct gggcgccctg tcgccacaga
     3841 cgcgcgccat gctggaggcg tacgccgccg gagtgaacgc gtggctttcg gcgaacccgc
     3901 ttccgacgga gtacacggcg ctcgagttga cgaaggcgac cgtgcccccg tggagcgctc
     3961 tcgactcggt cgcggcgacc aagctgctcg cgttcgacct gtcgttcgat atcagcgaca
     4021 tctcgaatac gcagaagctc atcgcctatg agaccgccgg cgctgccctc gggttcgacg
     4081 gagccaagct gttcaccgag ggtgtcatgc gcagcgagcc cttcgcgcac gcgccctcga
     4141 tcctccccgg tgaggcgtcc gagccgctac ggacgcacgg tcgcccggct tggtcgtcgt
     4201 ccgctctcgg acccggggtt ctcagtgcag cgaaggatgc gctcgagatg gcgaagaagg
     4261 ctggagtgcc gacgcacggt ctggagcagg gttcgaacgt gtgggttgtc tccggcgcaa
     4321 ggtccgcaac gggtcggccg atggtggcca acgacccgca cctctcgctc ggatcgccgc
     4381 ccgtcttcta cgaggtcggt atcgacgtct cgagcaatcc tgctcaggca ctgtcgctct
     4441 atggcgtgac cttcccagga acgccgttgg tcgtccacgg cacgaacggc cacgtctcat
     4501 ggggatcgac cgtgaacccc accgacgtca ccgacgtgta ccaggagcag ctcgttctcg
     4561 agggcgggat cccggtagcg acgatgtacc tgggggaatc cgaaccgacg cagatcatcc
     4621 cggagacgtt ccgcgccaat cagccgggga acgggagcgt cgacgacctc gtcgtggtcc
     4681 cacccgccgc cggggtccca gcagccacgg tggtcgttcc tcgccggaac aacggaccgc
     4741 tgatcagcgt cagcggctcc accggactct cggtccagta cactggcctc agcgcgacgc
     4801 gtgagccaga cttcttccgc ctgctctcgc gggctgcgac cgtcggggag gcgatcgacg
     4861 cccagcgcca ctttgacttc ggcgcccaga actggatgta cgtcgacgac cgcggcaaca
     4921 tcggctacaa gacgagcggc gagatcccgc tgcgcgagga tctctaggcc gagacggtcg
     4981 tcggtcttcc gccctatttc atccgcaatg gaacgggcgg caacgaatgg atcgccgacc
     5041 cggcgcccgc cgaggaccgg gcgctcgcct tccggatcct tccagtcgcc gagatggacg
     5101 ggctcgtcaa cccggcgcga gggtggatct cgaacgccaa tcaagatccg acagggcaga
     5161 cgttcgacaa cgacccgctc aacgagcttc ggccgggggg cggcattcgg tacatcacgc
     5221 ccggccacag cgacggcaac cgcaacgcgc ggatcaccga acgtctccag caggccctgg
     5281 ccgacggcgc ggtctcattc gcgcagatgc aatcgatcca ggccgatgtc gggctcaacg
     5341 acgccgccgt cctcgtcccg tacatcgtcg ctgcgcagca ggccgcgcag gccccgggag
     5401 cgcccccctc gctcgccgtc ctcggcgcgg acccccggct ggaggacgcg gtcgggcgcc
     5461 tctccgcctg ggacttctcc acgccgacgg gcctgcccga cggctacgac gcgggagacg
     5521 ctgacggcgt gcgatcgcca ccgacgcagg cagagatcga cgcgagcgtc gccgccacca
     5581 tctacggcct gtggcgcggc caggtgctca agctgatcgt
//
LOCUS       DASPWO010000179        11910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1212727, whole genome shotgun sequence.
ACCESSION   DASPWO010000179 DASPWO010000000
VERSION     DASPWO010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11910
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212727"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VEO00_04015"
     CDS             <1..619
                     /locus_tag="VEO00_04015"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57198.1"
                     /translation="AGRLVQVAEDGRTTAYADTQTLLTARTDPAGGTAFAYDSAGGTG
                     LLASVTDPFTAQATTYQYDAGGRPYRRIDASGLTTTRTYDGAGRIDGQTVLRTRTPTR
                     WRTSAWSATWPGTLPRRTPSSRAPGPRTGPGPIQDAFASLRDEEAQPLSREWSSPLER
                     PRFLLRTSSPALAGPTSLVAEPTAAEELQTVSKRDWSKRRSALIP"
     gene            complement(803..1624)
                     /locus_tag="VEO00_04020"
     CDS             complement(803..1624)
                     /locus_tag="VEO00_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYU57199.1"
                     /translation="MPRHRRHLRDREGDRPGACRDGGDHGPGRARPREGRFNVEEIRR
                     TTGRDRVEVLVADLSSQDSIRELAEVFRRDHDRLDVLVNCAGAFFRKRRVSADGLEMT
                     FALNHLAYFLLTNLLLDPLRRSGSARVLNVSAPATTKVDFEDLQSERRYRPFTAFGAS
                     KVANLMFTFELARRLEGTGITVNAVHPGRVRSNIMREAPAPFRLLTRLMSGSPERAGR
                     AIADLATSPEFAGTTGRFLRDGKEIRASADALNPALQRRLWELSEKLTGLDTETT"
     gene            complement(1647..2327)
                     /locus_tag="VEO00_04025"
     CDS             complement(1647..2327)
                     /locus_tag="VEO00_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HYU57200.1"
                     /translation="MKVGVLGTGTVGRAIGAWLLELGHDVTIGTRDVAALLASTEPNQ
                     ITQETFADWHAKNIAVGLGTFAEAAEHGELVVNATNGAGSLEALRAAGEANLDGKVLV
                     DIANPLDFSQGMPPSLFVSNTDSLGEQIQRAFPQVKVVKALNTMNAFVMADPSLVAGG
                     EHTVFLCGNDEEAKAQVTEILRSFGWRDVIDLGDITSARGTESYLPLWLRVWGALGTG
                     TFNIAVVQ"
     gene            2276..2923
                     /locus_tag="VEO00_04030"
     CDS             2276..2923
                     /locus_tag="VEO00_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYU57201.1"
                     /translation="MRRSRARRYRSREPRPSSGSGARERILETAGRLFYRDGYRAVGV
                     DTIIAESGVAKMTLYRHFRSKDELIAAYLERADRYLLQWIEGLIAPHADPRDALIGVF
                     DGVAKLASSPECLGCAFVGAAAEFPEPEHPGHRVALGHKRAVAASLRELAEAAGATDP
                     DELADELLLVMDGAWSASRVFGPGNHGRRAAEAARTLIGAHIDGDVPVPPGGSDP"
     gene            3250..4164
                     /locus_tag="VEO00_04035"
     CDS             3250..4164
                     /locus_tag="VEO00_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU57202.1"
                     /translation="MAEAAIRTEGLSKSFGATQALVSLDLDVPPGEVIGYLGPNGAGK
                     TTTIRLLLGLIGASAGSAEIFGIDCQADPVEAHRRVAYVPGEVSLWPSLTGTETLHLL
                     GRVQGRVDVAYRDVLLDRFEFDPTKKVRAYSKGNRQKPILIAGLMGRADLLLLDEPTS
                     GLDPLMERAFRACVADARERGQTVFLSSHILSEVEAVCDRIAILRAGHLVEVGTLAEM
                     RHLSALHVEADLDGTVPDLSGVPGVSAVQVEGSRVRCQVTGSVEPLLAVLARAGVRHL
                     VSEEPSLEELFLAHYGAGGSGEGSSDAA"
     gene            4154..5815
                     /locus_tag="VEO00_04040"
     CDS             4154..5815
                     /locus_tag="VEO00_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57203.1"
                     /translation="MLRRRQGRLRTRREAYDERRATRAMVVTTARRAARGGAFWGLVF
                     GATIAASATSYASLFPTAASRATVAVSFEGNAAWAALFGPLRRLDTVAGYTVYKSGMT
                     VIILGAIWGLLVATRVTRGEEDAGRWELFLSGRTTRARAAVQAAVGLGVGFAALWLPT
                     FLLTAGAGSSAEVGIGAGASAFFVTSLLAAAAMFVAVGIFSGQLAATRRDANLIGAGV
                     LAGSYLVRMAADSAPGLNWLRWASPLGWIEELQPLTGSRPYAFLPIAALVAVLVVVAL
                     RVAKGRGLGASAFASRDAPRPRTLLLGGQAGLTLRLTRPVVVAWIVALAITGLVFGLV
                     AQAAGTALRGAQGIEEAIARLGGTAGGAASYLGFVFVVAAGLMAIAVAGQVAATRNEE
                     AAGHLDNLFVRPVARWRWLAVRLGVGLGLSVVAGVVTGITAWVGAASQHADVGLGVLL
                     GAGLNVVPPAVFVLGVGGLAFGLWPRGSIGVAYGLVVWSFVVETFAAAFDSNHWLRDT
                     SPVLHIAPVPAADPNWGAAAWLVGLGLLAAVVGIARFTRRDLAGA"
     gene            complement(6309..9875)
                     /locus_tag="VEO00_04045"
     CDS             complement(6309..9875)
                     /locus_tag="VEO00_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYU57204.1"
                     /translation="MSDPPTVADTAPGATAGDVSCPACGTANPPENRFCGACGASLAV
                     PCAVCGAANPAGNRFCGSCGSPLTRPKAPSGDAPLEERKIVTIVFADLTASTEMAARL
                     DPEDLARVLKPFFEAMVAEIERFGGTVEKFIGDAIVAVFGAPVAHEDDPERAVRAALA
                     MQARLAALAPELAASSGQELAMRIGVNTGEVVTQIGPEHEGIVTGEPVNVAARLEAIA
                     DPGTIVVGERTHRDTADAIAYRALGNVVLKGIDHPVRAWVAEGDRPGDGRGRLAPLVG
                     RASELELLQLILSRTVRERRPGLVTIVGPAGIGKSRLAAELLERVAASSERVQVVRGR
                     CLPYGEGLTYWPLAEILKGDAEILDSDRPEEIPRKAAARLDVLLAGHEGHEGTVRVLL
                     SSIGVPVAPDPLAGAEPRAARELIARSWREYLESLGAGAPLVAIVEDLHWADPSLLEL
                     IEYLAARVEAPVLFLCTARPDLVDRRATWGGGQRNATTIELQPLSGAESADLLHGLVG
                     GKPLPADAMAAILERSEGNPFFAEELLRMVVEDGSLVERDGEWAVVRPLPSALPDTVQ
                     GVIASRIDMLEVGEKRAIQDAAVVGRIFWEGAVARLGLTDPAPAMEALAVKGLVRERD
                     RSVIEGERELIFHHVLTRDVAYASIPRSRRAEAHAAAGRWIEDVTRGRADEFAEILAY
                     HFDLAGDHSRHARYALLAGGRLRRVFSAPEAIRWYDRALSAADAAGDLETEAGAALGR
                     GTAHEQLGEFPQAEADYRRGLGRAREAGAAVTEARALAALAHVLWLQDRYEEGRRVQE
                     EALARSREVGATELEARLLYTAGTIAFGRGDFAEALGRHERGLAVSRATGDREGEALA
                     LHGLCETRWFTGPLDEALAAGRRADALLHELGQRPMVHHNEYMVGWVLWGTGAQEEAL
                     RWAERSAAGCREIGNRRDEAFALGTVAFLLNSLGRLADALRFGAEAVAVADEIGSPRL
                     RFAERVTLTTVLWDAGDTAGVEARVGEARGLLASLEGTFFASMLFGWECVLALSRGDR
                     TTARERLAAARVAPSEVERTFVAQTLIRGGESTGDAEVVREALALLESVPTANRPDLA
                     WAGYGRALLAVLEGDPLSAERLARESLAASDRVDPGPLGWRCRAALWRALRAQGRDAE
                     AAAAARDAANAFRELGAGLAADERDRFLAQQAAADLLSAAPA"
     gene            complement(9889..11106)
                     /locus_tag="VEO00_04050"
     CDS             complement(9889..11106)
                     /locus_tag="VEO00_04050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU57205.1"
                     /translation="MNEGARRALRRLGERAVQPARRMAESERAIDAYALVHAASVAGD
                     ALLALSLANSVFFSLPVDQAKTKVALYLLLTMAPLAVAAPLLAKVLDRGGFRRALTAT
                     AAVVRALLALLMASRTETLLLFPLAFGALVASRVHLVSKNALTASYAEEEEGLVAANA
                     LLSRVAAVTAVVVLAPGLAVGRIGGPGAVLFLAAVAYAGCALLVLRLPRAAEDPVAEV
                     PEGTNLVPGPVRVAAIGMAGVRAGGGFLIFLVAFTVRRVDQPAYWLGLVLGAVALGTL
                     LGAVVAPRMARARSEQGLVLAALGVAAVGSAAVATEFSLATLTLYALVAGLAGEVARL
                     AFQSLMQREVGRGAEGRAFVRFEVAFQLAWVGGAFVPALVSVTFRQGLLLMALLYGGA
                     AALNAVTRRRHAA"
     gene            11225..>11910
                     /locus_tag="VEO00_04055"
     CDS             11225..>11910
                     /locus_tag="VEO00_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HYU57206.1"
                     /translation="MGFEARDLPEDPLSGLDRAVARIAAASAEALSWVVAFDERGLWR
                     ADGATSMSAWLSARYGLAFGTAREWVRVAHRLRELPRIAAAYRAGRISFDQLRPLTRF
                     ATAESDGFWARRAPELRPSSLWREARRHERVSAREAQDAHRLRSLSLWRDPRAPLLLY
                     LEGILPGEQGAAFEAALSRAAEQVPADPDAEDRGGARLADALVALATASREAEPAPPT
                     LVVHADAAVLA"
BASE COUNT         1585 a         4372 c         4345 g         1608 t
ORIGIN      
        1 cgccgggcgc ctggtccagg tggcggagga cgggcggacg accgcctacg cagacaccca
       61 gacgctcctc accgcccgga ccgacccggc gggggggacg gcgttcgcat acgacagcgc
      121 gggggggacc ggcctgctcg cctcggtgac cgaccccttc accgcgcagg ccaccaccta
      181 ccagtacgac gccggcggcc gcccctaccg ccgcatcgac gcctccgggc tgaccaccac
      241 ccggacctat gacggggccg ggcggatcga cggccagacc gtgctgcgca cgcggacgcc
      301 cacgaggtgg cgcacttcgg cctggagcgc gacctggccg ggaacgttac cgcgaaggac
      361 gccttcgtcg agggcgccgg ggccgagaac ggggcctggg cctatccagg atgcgtttgc
      421 gtcgctgcgc gacgaggagg cccagccgtt atcacgggaa tggtcttcac cgctggaaag
      481 accacggttc ttgttacgga cttccagccc cgcactggca ggcccgacat cgctggtggc
      541 cgaaccgacg gcggccgagg aattgcagac tgtttcgaaa cgggactggt ccaagaggcg
      601 ctcggcgttg atcccttgaa cccagtgccc ccgaaagaac catcttcagg attctccgac
      661 ttcctgatcg tccatcacgg atccgtcacg tcgaaacgtc tgtcgccggc ccggagggat
      721 caggcggttg cagtgccaaa gaccgacgtc ttcgatccgc tcgaacgcga tggcgtggcg
      781 cctggtcccg ccctggtgac cctcacgtcg tctcggtgtc caggcccgtg agcttctcgc
      841 tcagctccca gagccggcgt tgaagcgctg ggttcaaagc atcggcgctc gcgcggatct
      901 ccttcccgtc gcgaaggaag cggccggtcg tcccggcgaa ctccggggag gtggcaaggt
      961 ccgcaatcgc ccggccagcg cgctcggggg agcccgacat caggcgtgtg aggagccgga
     1021 acggcgcggg tgcctcgcgc atgatgttgg agcgcacgcg tcccgggtgg actgcgttca
     1081 ccgtgatgcc cgtaccttcg agccgacgcg ccagctcgaa cgtgaacatc aggttcgcca
     1141 ccttcgatgc gccgaaggca gtgaagggcc ggtaccgacg ctcgctttgc aggtcctcga
     1201 agtcgacctt cgtcgtggcg ggggccgaga cgttcaggac ccgcgccgac ccacttcgcc
     1261 tcagcgggtc gagaagcagg ttggtgagca ggaagtaggc cagatggttc agcgcgaacg
     1321 tcatctcgag accgtcggcg ctcacgcggc gcttccggaa gaaggcgccg gcacagttga
     1381 cgagcacgtc gaggcggtcg tgatcgcggc ggaacacctc ggccagctcg cggatcgagt
     1441 cctgtgagga caggtcggcg acgagtacct cgacccggtc gcgcccggtc gtccgccgga
     1501 tctcctcgac gttgaatcgc ccctcgcgcg gtcgtgcgcg accaggaccg tggtcgcccc
     1561 catcgcggca agcgcctggg cggtcgcctt cccgatcccg gaggtggcgc cggtgacgag
     1621 gcagtcccgg tcgaggatct ggccggtcac tgcacgaccg cgatgttgaa cgtcccggtg
     1681 ccgagcgccc cccacacgcg gagccacagc ggcaggtagc tctcggtccc gcgcgccgag
     1741 gtgatgtcgc cgaggtcgat cacgtcgcgc cagccgaacg accgcaggat ctcggtgacc
     1801 tgggccttgg cctcctcgtc gttcccgcag aggaagacgg tgtgctcacc tccggcgacg
     1861 agcgaggggt cggccatcac gaacgcgttc atcgtgttca acgccttcac gaccttcacc
     1921 tgggggaacg cacgctggat ctgctcaccg agcgagtccg tgttcgagac gaacaaggac
     1981 ggcggcatgc cttgggagaa gtccaggggg ttggcgatgt cgaccaggac cttgccgtcg
     2041 agattcgcct caccggccgc gcgtagtgcc tccagcgaac cggcgccgtt cgttgcgttg
     2101 acgacgagct cgccgtgctc ggccgcctca gcgaaggtcc cgagtcccac ggcgatgttc
     2161 ttggcgtgcc aatcggcgaa cgtctcctgc gtgatctggt tgggctcggt cgacgcgagg
     2221 agcgccgcca cgtcccgcgt gccgatcgtg acgtcgtgac cgagctcgag gagccatgcg
     2281 ccgatcgcgc gcccgacggt accggtcccg agaaccccga ccttcatcag ggtccggtgc
     2341 cagggagcgg atcctggaga cggccgggcg gctgttctac cgtgacggct accgcgcggt
     2401 gggcgtggat acgatcatcg ccgagtccgg tgtggcgaag atgacgctgt accggcattt
     2461 ccgctcgaag gacgagctga tcgccgccta cctggagcgg gccgaccgtt acctgctcca
     2521 gtggatcgag gggctgatcg caccccacgc ggaccctcgc gacgccctga tcggggtatt
     2581 cgacggggtg gcgaagctcg cgtcgagccc cgagtgcctg gggtgcgcgt tcgtcggagc
     2641 tgcggcggag ttccccgagc ccgagcaccc cggccatcgc gtcgcgctcg ggcacaagag
     2701 ggccgtggcg gcgagcctgc gcgagcttgc cgaggcggct ggagccaccg atcccgacga
     2761 gctcgccgac gagctgctgc tcgtgatgga cggcgcatgg agcgcgtcgc gggtgttcgg
     2821 cccggggaac cacggccgac gcgcggccga ggcggctcgc acgctcatcg gcgctcacat
     2881 cgacggtgac gtccccgtgc ctcccggagg ctccgacccg tgagaggttg gagccatgtc
     2941 acgaccagga aggtacccgc aggaactgca tgagcgagcc gtccgcatgg ttcgagcacc
     3001 aggacgagca cccatcccag tggagaccct gctcggctac cagggcgacc cgaccgaccg
     3061 cgacaccgtc ctggtcgaca tggtcagccg gctgtacgac cggacgctgc agatggagcc
     3121 ggactgaact cggccagggc cggcacgaag tccttggcgg acagcccgtg caggtacagc
     3181 aggggcagga cgccgcgccg gtgcggccgg atgctgaact ttttagatac ttctgaaaga
     3241 gctgagcata tggcggaggc ggcgatccgg accgaggggc tgtcgaagtc cttcggcgcc
     3301 acgcaggcgc tggtctccct ggacctggat gtgcccccgg gcgaggtgat cggctacctc
     3361 gggccgaacg gcgccgggaa gaccaccacg atccggctgc tgctgggcct gatcggggcc
     3421 tcggcgggca gcgcggagat cttcggcatc gactgccagg ccgatccggt cgaggcccat
     3481 cgccgcgtcg cctacgtgcc cggcgaggtc agcctgtggc cgtcgctcac gggaacggag
     3541 acgctccacc tgctgggccg cgtgcagggc cgagtggacg tcgcctatcg ggacgtgctg
     3601 ctggatcggt tcgagttcga tccgaccaag aaggtgcggg cctactcgaa gggcaaccgg
     3661 cagaagccga tcctgatcgc cgggctgatg gggagggccg atctgctcct cctcgacgag
     3721 ccgacgagcg ggctcgatcc cctgatggaa cgggcgttcc gcgcctgcgt cgcggacgcg
     3781 cgcgagcgcg gccagacggt gttcctgtcc tcgcacatcc tctccgaggt cgaggcggtg
     3841 tgcgaccgca tcgccatcct gcgcgccggc cacctggtgg aggtgggcac gctcgccgag
     3901 atgcgccacc tctcggcgct gcatgtggag gccgacctgg acggcaccgt gcccgacctg
     3961 tcgggcgttc cgggggtgag cgccgtgcag gtggagggaa gccgcgtccg ctgccaggtc
     4021 accgggtcgg tggagccgct gctcgccgtg ctggcccgtg ccggcgtgcg ccacctcgtg
     4081 agcgaggagc cgtcgctcga agagctgttc ctggcccact acggcgctgg cggctcgggg
     4141 gagggttcct ccgatgctgc gtaggcgcca ggggcggctg cgaacgcgac gggaggccta
     4201 cgacgagcgg cgcgccacac gcgcgatggt cgtcacgacc gcgcggcgtg ccgcacgagg
     4261 cggggccttc tgggggctgg tgttcggagc cacgatcgcg gcctcggcga cctcgtacgc
     4321 ctcgctgttc ccgaccgcgg cctcgcgggc tacggtggcg gtgtcgttcg aggggaacgc
     4381 cgcgtgggcg gcgctgttcg ggccgctccg ccggctcgac acggtggcgg gctacaccgt
     4441 ctacaagtcg gggatgacgg tgatcatcct cggggcgatc tggggcctgc tggtcgccac
     4501 ccgggtgacc cgcggcgagg aggacgccgg ccgctgggag ctgttcctgt ccgggcggac
     4561 gacccgggcg cgggcggccg tgcaggcggc ggtcgggctc ggcgtcgggt tcgcggcgct
     4621 ctggctgccg acgttcctgc tgaccgcggg cgcgggatcg tcagccgagg tgggtatcgg
     4681 cgccggggcc tcggcgttct tcgtcacgtc gctcctcgcc gccgcggcga tgttcgtcgc
     4741 cgtcggcatc ttctccgggc agctcgccgc gacgcggcgc gacgccaacc tgatcggcgc
     4801 cggtgtgctg gcgggctcgt acctggtccg gatggccgcc gactcggcgc cgggactgaa
     4861 ctggctgcgc tgggcgagcc cgctggggtg gatcgaggag ctccagccgc tcacggggtc
     4921 gagaccctac gcgttccttc ccatcgccgc cctcgtggcg gtgctggtgg tcgtggcgct
     4981 tcgggtggcg aagggacggg gcctcggcgc gagcgccttc gccagccggg acgcgccgcg
     5041 accgcgaacg ctcctgctcg gggggcaggc cgggttgacc ctgcggctga cgcgcccggt
     5101 ggtcgtggcc tggatcgtgg cgctcgccat caccggtctg gtcttcggac tggtcgccca
     5161 ggcggccggg accgcgcttc ggggcgcgca ggggatcgag gaggcgatcg cgcggctcgg
     5221 ggggaccgcg ggcggggcgg cctcgtacct ggggttcgtg ttcgtggtcg cggcggggct
     5281 gatggcgatc gcggtggccg gccaggtcgc cgcgacgcgg aacgaggagg cggccggtca
     5341 cctggacaac ctgttcgtcc gccccgtggc gcgctggcgg tggctggcgg tcaggctggg
     5401 agtgggcctc gggctctccg tcgtcgccgg cgtggtgacc gggatcacgg cctgggtcgg
     5461 ggccgcgagc cagcacgccg acgtcggcct cggcgtgctg ctgggagccg gcctgaacgt
     5521 cgtgcccccc gcggtgttcg tgctcggggt cggcgggctc gccttcggcc tgtggccgcg
     5581 gggctcgatc ggcgtcgcct acgggctggt cgtgtggtcg ttcgtcgtcg agacgttcgc
     5641 cgcggccttc gactccaacc actggctgcg cgacacgtcg ccggtgctgc acatcgcccc
     5701 ggtgccggcc gcggacccga actggggagc cgcggcctgg ctggtcggcc tcggcctgct
     5761 ggcggcggtg gtcgggatcg ctcggttcac tcggcgcgac ctggccggag catgaccgtc
     5821 gccaccgccc caccggtgcg gcgctccctg gccggctggc aggccgagtt cctggagcgc
     5881 gtcgccgtct gggccgacgt gagcggcctg ccgcccagct ggatccgcct gttcgcgtgg
     5941 ctgatcgtgt gcgacccgcc cgagcagtcg gtcgaggacc tccgcgacgt actcgagctc
     6001 tccgccggat ccatcagcat ggccaccgcc gccctgggcc ggatgggtct catcgagcgc
     6061 atccgtcgcc ccggcgaacg gcgactccgc taccgcttcc accctcacgg gtgggagcgg
     6121 atcctgcgcc tccggttgga ggcgacgtcc aacgtccggg ccgccgccga ggacgcgctc
     6181 gaacaggccc cggctcgcga tccccgccct caccgagatg cgcgacctct acgcctactt
     6241 cgagcggtcc gtcgcagggt tcctgagcgg gcttccggcg gcacggactc cggcccgccg
     6301 ctgaggcgct acgcgggcgc ggccgacagc aggtcggccg ctgcctgctg cgccaggaat
     6361 cggtcacgct cgtcggcggc gagtcccgca ccgagctccc ggaaggcgtt cgcggcgtcg
     6421 cgggccgcgg cggccgcctc cgcgtcgcgg ccctgggccc gcagggcccg ccagagtgcc
     6481 gcgcggcacc gccagcccag ggggccgggg tcgacccggt cgctcgcggc gagcgactcc
     6541 cgcgccaggc gctcggcgga gaggggatcc ccctccagaa cggcgagcag cgcccgcccg
     6601 tagcctgccc aggccaggtc cgggcggttg gcggtcggca ccgactcgag gagggcgagg
     6661 gcttcccgga cgacctcggc gtccccggtc gactcgccgc cccgtatcag ggtctgggcg
     6721 acgaacgtcc gttccacctc cgacggtgcc acccgggccg cggcgaggcg ctcccgcgcc
     6781 gtcgtccggt cgccacgcga cagggcgagg acgcactccc aaccgaagag catcgaggcg
     6841 aagaaggtgc cctccaggga cgccagcagc ccgcgcgcct cccccaccct ggcctccacc
     6901 cccgccgtgt cgcccgcgtc ccacaggacc gtcgtcagcg tcacccgctc ggcgaaccgc
     6961 aggcgcggcg atccgatctc gtcggccaca gcaaccgctt ccgcgccgaa gcgcagcgcg
     7021 tcggccaggc gtccgagcga gttcaggagg aacgcgaccg tgcccagcgc gaaggcctcg
     7081 tcccggcggt tcccgatctc gcggcatccc gccgcggatc gttccgccca gcgcagcgcc
     7141 tcctcttgcg cgccggtccc ccacaggacc cagcccacca tgtactcgtt gtggtggacc
     7201 atcgggcgct gcccgagctc gtggagcagg gcgtcggcgc gccgcccggc ggcgagggcc
     7261 tcgtcgagcg gaccggtgaa ccaccgcgtc tcgcagagcc cgtgcagggc gagcgcctca
     7321 ccctcgcggt caccggtggc gcgggagacc gcgagcccac gctcgtgccg accgagcgcc
     7381 tccgcgaagt ccccccggcc gaaggcgatc gtcccggccg tgtagagcag gcgcgcctcg
     7441 agctccgtcg ctcccacctc gcgcgaccgc gccagcgcct cctcctgcac ccggcggccc
     7501 tcctcgtagc ggtcctgcag ccacagcacg tgggccagcg ccgccagggc ccgtgcctcg
     7561 gtcaccgccg ccccggcctc cctcgcccgc ccgaggcctc gccggtagtc ggcctcggcc
     7621 tgcgggaact ccccgagctg ctcgtgcgcg gtcccccggc cgagcgcggc gcccgcctca
     7681 gtttcgaggt cgccggcggc gtctgcggcg gagagcgccc ggtcgtacca gcggatcgcc
     7741 tcgggcgcgg agaacaccct gcgcagccgc ccgccggcga ggagggcgta gcgcgcgtgg
     7801 cgggagtggt cgccggccag gtcgaagtgg taggcgagga tctccgcgaa ctcgtcggct
     7861 cgcccgcggg tgacgtcctc gatccagcgg cccgccgccg cgtgcgcctc cgcccgccgc
     7921 gaccgcggga tgctcgcgta cgcgacgtcg cgggtcagga cgtggtggaa gatcagctcg
     7981 cgctccccct cgatcacgga ccggtcccgc tcgcggacca ggcccttcac ggcgagggcc
     8041 tccatcgccg gcgccgggtc ggtcaagccg aggcgggcca ccgcgccctc ccagaagatc
     8101 cgcccgacga cggccgcgtc ctggatggcg cgcttctcgc cgacttcgag catgtcgata
     8161 cgcgacgcga tcacgccctg cacggtgtcg ggcagcgccg aggggagggg cctgacgacc
     8221 gcccattcgc cgtcgcgctc gacgagcgag ccgtcctcga ccaccatgcg cagcagctcc
     8281 tccgcgaaga acgggttccc ctcgctccgc tccaggatcg cggccatcgc gtcggcgggg
     8341 agtggcttcc ccccgaccag cccgtggagc aggtcggccg actcggcgcc ggagagcggt
     8401 tgcagctcga tcgtggtggc gttgcgttgg ccaccgcccc aggtggctcg gcggtcgacc
     8461 aggtcgggcc gggcggtgca caggaagagc accggcgctt cgacccgcgc cgcgaggtac
     8521 tcgatcagct cgaggaggct gggatcggcc cagtgcaggt cctcgacgat cgcgacgagc
     8581 ggcgcgccgg ccccgagcga ctcgaggtac tcccgccacg accgggcgat cagctcgcgg
     8641 gcggccctcg gctccgcccc cgcgagcggg tcgggcgcca cggggacgcc gatcgaggac
     8701 agcagcacgc gcaccgtccc ctcgtggccc tcgtggccgg cgaggagcac gtcgaggcgt
     8761 gccgccgcct tgcgcgggat ctcctccggg cggtcgctgt ccaggatctc ggcgtccccc
     8821 ttcaggatct cggcgagcgg ccagtaggtc agcccctcgc cgtacggcag gcagcgcccg
     8881 cgcaccacct ggacgcgctc ggacgacgcc gcgacccgct cgagcagctc ggccgccagc
     8941 cggctcttcc cgatcccggc cgggccgacg atggtcacca ggccggggcg gcgctcgcgc
     9001 accgtccggg acaggatcag ctgcaggagc tccagctcgg aggcccgccc gacgagcggc
     9061 gccaaccgcc cgcggccgtc gcccggccgg tcgccctcgg ccacccacgc ccggaccgga
     9121 tggtcgatcc ccttcaggac cacgttgccg agcgcgcggt aggcgatcgc gtcggcggtg
     9181 tcgcggtggg tccgctcgcc gacgacgatg gtcccgggat cggcgatcgc ctccagccgg
     9241 gcggcgacgt tgaccggctc cccggtgacg atgccctcgt gctccggccc gatctgggtg
     9301 acgacctcgc cggtgttcac accgatgcgc atcgccagct cctgccccga ggaggcggct
     9361 agctccggcg cgagcgccgc gagacgcgcc tgcatcgcga gcgccgcgcg caccgcgcgc
     9421 tccgggtcgt cctcgtgcgc gaccggtgcc ccgaacaccg cgacgatcgc gtcgccaatg
     9481 aacttctcca cggtgccgcc gaagcgctcg atctccgcaa ccatcgcttc gaagaagggc
     9541 ttcagcacgc gcgccaggtc ctcggggtcg aggcgcgcgg ccatctcggt cgaggccgtg
     9601 agatcggcga agacgatcgt gacgatcttt cgctcttcga gcggcgcgtc gccggacggt
     9661 gccttcggtc gcgtcagcgg ggacccgcac gagccacaga agcggttgcc ggccgggttc
     9721 gccgcgccgc agaccgcgca ggggacggcg agggacgcgc cgcacgcgcc gcagaagcgg
     9781 ttctccggcg ggttggccgt cccgcaggcc gggcagctca catcgccggc ggtggctccg
     9841 ggcgcggtgt ctgcgacggt cggcggatcc gacatctccg cgcagaggct acgccgcgtg
     9901 gcgacggcga gtcacggcgt tcagcgcggc cgccccgccg tacagcagcg ccatcagcag
     9961 gagcccctga cggaacgtga cggagacgag cgccgggacg aacgcgccgc cgacccacgc
    10021 gagctgaaac gcgacctcga accggacgaa cgcgcgaccc tcggccccgc gcccaacctc
    10081 gcgctgcatc aggctctgga aggccaggcg cgccacctcg ccggccaggc ccgcgaccag
    10141 cgcgtacagc gtcagggtgg ccaggctgaa ctcggtggcg acggccgccg agccgaccgc
    10201 cgcaaccccc agcgcggcca ggaccaggcc ctgctcgctg cgcgccctgg ccatcctcgg
    10261 cgcgacgacg gccccgagga gcgtgccgag cgccacggcg ccgagcacca ggcccagcca
    10321 gtacgcaggc tggtccaccc ggcggaccgt gaacgccaca aggaagatca ggaagccgcc
    10381 gccggcccgc accccggcca tcccgatcgc ggccacccgc accgggcccg ggacgaggtt
    10441 cgtgccctcg gggacttcgg ccacggggtc ctcggcggcg cgcggcaggc gcaggacgag
    10501 cagcgcgcac cccgcgtagg ccactgcggc caggaacagc accgcaccgg gcccgccgat
    10561 cctgccgacg gcgaggccgg gtgcgaggac gacgacagcc gtcaccgccg cgacccgcga
    10621 caggagcgca ttcgccgcga ccaggccctc ctcctcctcg gcgtaggagg cggtgagcgc
    10681 gttcttcgag acgagatgga cgcgcgaggc gaccagcgcg ccgaaggcca ggggaaagag
    10741 caggagcgtc tccgtgcggc tcgccatcag gagcgccagg agtgcccgga cgacggccgc
    10801 cgtagcggtc agggcgcggc ggaagccgcc gcgatcgagg accttcgcca ggagtggcgc
    10861 agccaccgcc aggggggcca tcgtcagcag caggtagagg gcgaccttcg tcttcgcctg
    10921 gtcgaccggc aaggagaaga acactgagtt ggcgagcgac agggccagca gcgcgtcgcc
    10981 tgccacgctc gccgcgtgca cgagagcgta tgcgtcgatc gcccgctccg actcggccat
    11041 ccgccgcgcc ggctggaccg cccgctcccc cagccggcga agcgcacgtc gcgctccctc
    11101 gttcacgcgc gaagcatagg gagaaggcga cgggtcaccg gggcgtgatc tcgttcacat
    11161 ccggggagtg gagggggcct ccacgaccca aaacgtgtca gaccccctcg ctagagttac
    11221 agggatggga ttcgaagccc gcgaccttcc cgaggacccg ctttcgggcc tcgatcgcgc
    11281 cgtggcgcgg atcgccgcgg cctccgccga ggcattgtcc tgggtggtcg cgttcgacga
    11341 gcgagggctc tggcgagccg acggggccac ttcgatgagc gcctggctct ctgcccgcta
    11401 cggcctggcc ttcggcacgg cccgggagtg ggtccgggtg gcccaccggc tccgggagct
    11461 ccctcggatc gccgcggcct accgcgccgg gcggatctcc ttcgaccagc tccggccgct
    11521 gacccgcttc gccacggcgg agtccgacgg cttctgggcg cgccgggccc cggagctccg
    11581 gccctcctcg ctgtggcggg aggcccgccg gcacgaacgt gtctcggccc gagaggcgca
    11641 ggacgcccac cggctgcgct ccctgtcgct gtggcgggac ccgcgggccc cgctgctgct
    11701 gtacctcgag gggatcctcc ccggcgagca gggcgcggcc ttcgaggccg ccctctcccg
    11761 ggccgccgag caggtgccgg ccgacccgga cgccgaggac cgcggcggcg cgaggttggc
    11821 cgacgcgctc gtggcgctgg ccaccgcctc tcgtgaggcc gagccggccc cgccgaccct
    11881 ggtggtccac gccgacgccg ccgtgctcgc
//
LOCUS       DASPWO010000180         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_109890, whole genome shotgun sequence.
ACCESSION   DASPWO010000180 DASPWO010000000
VERSION     DASPWO010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109890"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /gene="rplQ"
                     /locus_tag="VEO00_04060"
     CDS             <1..362
                     /gene="rplQ"
                     /locus_tag="VEO00_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HYU57207.1"
                     /translation="PKKGPRLGSGPAHQRLMLGGLAASLFVHERIRTTEAKAKALRPL
                     AEKLITFAKDGSVHARRQVLRVVPDRDVVHKLFAEIAPRFEERPGGYTRIMKLGPRKG
                     DGAPMALIELVERATAE"
     gene            416..1186
                     /gene="truA"
                     /locus_tag="VEO00_04065"
     CDS             416..1186
                     /gene="truA"
                     /locus_tag="VEO00_04065"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HYU57208.1"
                     /translation="MAYDGTGFRGWARQPRLRTVQGVLEDALADLLGVAPRLSVAGRT
                     DAGVHARGQVVSFEAHAEPARLQRALNGRLAPEVVVLRADRAPAGFDARFSATGRRYR
                     YRIDTAPLPDPFTARYVWHRPGDLPLAPMRRAARDLVGTHDFASFCRAPRAPASTVRT
                     LHRLSVAMREGLVTVTADADGFLQQMVRSLAGTLVDVAIGRIEPSAMPAVLTARDRAA
                     AGRVAPPHGLTLEAVRYRRNERGSRYESGRAGTSAGLC"
     gene            1194..1613
                     /locus_tag="VEO00_04070"
     CDS             1194..1613
                     /locus_tag="VEO00_04070"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HYU57209.1"
                     /translation="MEPPLDEEEFRRWRDEAGRALEGARVQAGAGLHNWACFAAEQAA
                     QLALKALLHGLGRGPWGHDLVRLGELAGEAGLVVPEALAGRLRRLGRHYIPARYPDAH
                     AAGPPSAHYASTDSDEAAEDAGEVLSFVDRTWKELHG"
     gene            1606..1971
                     /locus_tag="VEO00_04075"
     CDS             1606..1971
                     /locus_tag="VEO00_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYU57210.1"
                     /translation="MADPVAERIRRRDALLAAARDYVDRLSRRAPVVAAAVVGSVARG
                     DFNVWSDVDVVVVADDLPDRIPDRGLVLAADARMGVQAVGFTPREFERAHRRGDPLAR
                     EATTSGVVLRGEGYLRRFH"
     gene            2033..2467
                     /gene="rplM"
                     /locus_tag="VEO00_04080"
                     /pseudo
     CDS             2033..2467
                     /gene="rplM"
                     /locus_tag="VEO00_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473395.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
BASE COUNT          379 a         1026 c         1026 g          339 t
ORIGIN      
        1 cgccgaagaa gggaccgcgg ctcggcagcg ggccggccca ccagaggctg atgctcggcg
       61 ggctcgcggc atcgctgttc gtgcacgagc gcatccgcac caccgaggcc aaggccaagg
      121 cgctgcgccc gctggcggag aagctgatca cgttcgccaa ggacggctcg gtgcacgccc
      181 ggcggcaggt cctgagggtg gttcccgacc gcgacgtggt gcacaagctg ttcgcggaga
      241 tcgcgccgcg gttcgaggag cgccccggcg gctacacgcg gatcatgaag ctcggtccgc
      301 gcaagggcga cggcgcgccg atggcgctca tcgagctggt cgagcgcgcg acggccgagt
      361 aggcgcgacg gcgccggacc cccgccccca gcccgccccg atcgtccggc tgaccctggc
      421 ctacgacggc accgggttcc gcggctgggc gcgacagccg cgcctgcgca cggtccaggg
      481 cgtcctggag gacgcgctgg ccgacctcct gggcgtggcc ccacgccttt ccgtggccgg
      541 gcgcaccgac gccggcgtcc acgcccgtgg tcaggtggtg tcgttcgagg cgcatgccga
      601 gcccgcccgg ctccagcgcg cgctgaacgg ccgcctggcg ccggaggtgg tggtgctccg
      661 ggcggaccgt gccccggccg ggttcgatgc ccgcttctcc gccaccggcc ggcggtaccg
      721 ctacaggatc gacacggcgc cgttgcccga cccgttcacc gcccgctacg tctggcaccg
      781 gccgggcgac ctcccgctcg ccccgatgcg ccgcgccgcg cgcgacctgg tcgggacaca
      841 cgacttcgcc tcgttctgcc gggccccgcg ggcgccggcc tcgaccgtgc gcacgcttca
      901 ccgcctgtcg gtggcgatgc gcgagggcct ggtgaccgtg acggccgatg ccgacgggtt
      961 cctgcagcag atggtgcgca gcctggccgg gacgctggtc gacgtggcga tcggacggat
     1021 cgaaccctcg gccatgcccg ccgtgctcac cgcgcgcgac cgagcggccg ccggccgggt
     1081 cgccccgccc cacggcctga cgctcgaggc cgtcaggtac cggcggaacg aacgcggatc
     1141 gcggtacgag agtggccggg ccggcacgag cgcggggcta tgctgacgcc gacgtggaac
     1201 ccccgctcga cgaggaggag ttccgtcgct ggcgcgacga ggccgggcgt gccctcgagg
     1261 gggcccgggt ccaggccggt gccgggctcc acaactgggc ctgcttcgcg gcagagcaag
     1321 ccgcccagct tgccctcaag gcccttcttc acggcctcgg ccggggtccc tggggccacg
     1381 acctggtccg gctgggcgag ctggccggcg aggccggact ggtggttccg gaagccctcg
     1441 ccggaaggct gaggcgactc ggacggcact acatccccgc ccggtacccc gacgcccacg
     1501 ccgcaggacc gccgtcagcc cactacgcct ccaccgactc cgacgaggcg gctgaggacg
     1561 ccggcgaggt cctgtccttc gtggatcgaa cctggaagga gctccatggc tgatcccgtc
     1621 gccgagagga tccgccggcg cgatgccctg ctggccgcgg cgagggacta cgttgatcgc
     1681 ctctcccgcc gggctccggt cgtggccgcg gccgtcgttg ggagcgtggc tcgaggcgac
     1741 ttcaacgtct ggagtgacgt ggacgtcgtg gtggtggcgg atgacctccc cgaccggatc
     1801 cccgatcgcg gcctggtgct cgccgccgat gctcgcatgg gcgtgcaggc ggtgggattc
     1861 acccctcggg agttcgagcg cgcccatcgt cgcggcgacc ccctggcccg cgaggcgacg
     1921 acctcggggg tcgtcctgcg gggggagggc tacctgcgcc ggttccattg acgctcgggc
     1981 gtttccgcag gtagactcgg ccatcggccg ccaggctcgc ggccgttttc ttatgaagac
     2041 cttcagtccg aaacccgagc acatcgagcg ccgctggtac gtcgtcgacg ccgagggcgc
     2101 cgtcctggga cggctggcca cgcacgtggc gtcgctgctg cgcggcaagc acaagccgat
     2161 ctgggccccg cacgtggacg ccggcgacca cgtcgtcgtg gtcaacgccg ggaaggtccg
     2221 cctgacgggg acgaaggacc agtcgaagac ctaccgcacg cactcgcagc acccgggcgg
     2281 gctgcgcacg gtggacttcg agcggatgct ggccacccgg ccgcacgtcg tcgtcgagcg
     2341 ggccgtgcgc gggatgctcc cgaagaaccg cctcgggcgc cagatgctcc gcaagctgtc
     2401 ggtctacgcc gggccgcagc accgccacgc ggcccagaag ccggagccgc ttcagatcgg
     2461 cgaggttcct ccctggagcg ggctcccggc tcccaagccg aagcccgagc ccgggcccga
     2521 cgaggagacc cgccgccccc gcggccgccg cgccggcagg agcgctcccg cgaaggcggg
     2581 cccggccagg aaggcgacgg tgaggaaggc aggggccaag cgtgccccgg ccaaggcggc
     2641 ggcccggagg ccggcgaagg cgagtgcgaa gaaggcgacc gcgaagcgaa ccgcgaggaa
     2701 gaccgccccg aagcggtcgg cccgccgaac cacgaaggag tcataagtgg cggagaccgc
     2761 acagctcacc
//
LOCUS       DASPWO010000181         7971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_27751, whole genome shotgun sequence.
ACCESSION   DASPWO010000181 DASPWO010000000
VERSION     DASPWO010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27751"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VEO00_04085"
     CDS             complement(<1..1108)
                     /locus_tag="VEO00_04085"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HYU57211.1"
                     /translation="MTLTNEAEYVLRTVEERGIKFIRLWFTDVLGFLKSFAITPSELE
                     GAFAEGMGFDGSSIEGFSRIQESDMVARPDPATFQIIPWKSEAQVARMFCDIYTPDGE
                     PFGGDPRFVLKRQLDRAADLGFTFFVGPELEYFYFRDSETTQFLDRGGYFDLTPLDVA
                     TDYRKRTVFYLESMGIPVEYVHHEVAPSQHEIDLRYTDALTMADNVMTYRLTVKEAAQ
                     EFGVYATFMPKPVDGVNGSGMHTHQSLFEGERNAFFDATDEYHLSKTAKGYIAGLLTH
                     AREITLVTNQWVNSYKRLVPGYEAPVYVCWARRNRSALVRVPMYKPGKEQATRIEFRS
                     PDPACNPYLAFAAMLAAGLKGVEEEYDLPAEASNN"
     gene            complement(1193..2293)
                     /locus_tag="VEO00_04090"
     CDS             complement(1193..2293)
                     /locus_tag="VEO00_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HYU57212.1"
                     /translation="MNGCPTGTTARRRAAAAVAWVVLFSACPAKPAPPPPEPSVVTPT
                     FDQIRQDGWTRVEPGGRTLCARGDPFAYFVHRGDPSRLLVFFTGGGACQDGATCRPGS
                     RFFRDTVRYGPDGPPANGILDFGDPRNPFRDYTAVVIPECTGDLHWGDRDVAYVSPLG
                     GSFTIYHRGWVNARSALRWASTNVPSPAEVFVAGCSAGSIGSAAHAPFVARRYPEARV
                     TQLGDSFAGVFPKPIDLDAPYGAGRHLATWLAPVRDLDPKRLTMTDYYRALVRAIPDQ
                     TFSEFSHDNDLAQKLYFLLLGGFPRQFSRALRVSFDAIETAGPNFSSYIAAGNGHCVL
                     QTPSFYSEGSRVPILDWVTALASDGQAPTVRP"
     gene            2384..5032
                     /locus_tag="VEO00_04095"
     CDS             2384..5032
                     /locus_tag="VEO00_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HYU57213.1"
                     /translation="MTTPEVLGRALAAAPDPERARVALSRVGEDPRARETLADPAVLA
                     AAVPLLGLSTAVTDFLMAHPEEATLFAALEPRDHATLMEEARADAARLGASAGLRRFR
                     RRALARVAAADLAGRPFEPVVAEVTAVAEACLQVALEAVDGAGLAVVGMGKLGGRELN
                     YASDVDVVFVHGRPGGEPQEAASRVAGALLRLLAEPTDEGLALRVDADLRPEGRAGAL
                     SRSLAAMGEYYERHAETWERQAMVKARPVAGDAALGAAFLDAVTPLVFPERLAPAAIE
                     DVRRMKVRVEEYVRARGRHDVKRGPGGIRDVEFAVQLLQLVHGRRDPSLRTTNTLEAL
                     RALAEGGYVALADAVALADSYRFLRTLEHRLQLLREVQAHDLPEDEQTLRTVARAMGL
                     AGAAALRKSVEGHAGVVRALHEGLFYRPLLEAFAGDEPRPGVDREATQELLAGLGFRD
                     PAGAYARLEAIVDPSTRLGRVLGTSFPLLGPALALAANPDAALVRFARVAEPLREAPG
                     LADRLASQPETLQDLASLVAASSAFADVVVRRPDVVPVLGPAASPPDPAVDPEAALVR
                     VAARYASGNLPVPESGRAIAAVADAVVRRAVRAGAPALPFAAIALGKLGSEELNFASD
                     LDLVFVYEGEGAEAFGEAARAAEATLAAIRADGFAPDPDLRPEGRAGPLVRSVASYLE
                     YWERWALTWEFQALLKARPVAGDEVLGRRFRSLAEDFAYPEALPIDRVAEIRKMRVRM
                     EQERVRPREAGRFHFKLGFGGLADVQFAVELSQMRHGRDHPAVRERHTLMALERLVEA
                     RLIEDSVARSLGEAYLFLNEVKNALEIDKRVTAEAVPPAPEEQAALARRLGYEEYPRA
                     RFLEEYRRITRRARMAMERVFYGEDE"
     gene            5029..5544
                     /locus_tag="VEO00_04100"
     CDS             5029..5544
                     /locus_tag="VEO00_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57214.1"
                     /translation="MRVRFGAALALAALTLAACGGAGGNAGEGGIEFREIGSGGVPGQ
                     ERLTPEALVLRSVAEARDRLEGRVDAQDLESVASLDFGDRALVAVFAGMGNSPAVEAH
                     VKAVRVRDRALEIEVNVRQQGGPAIEALVGLYVLVDVAAADVEGIERGTVEVKTVKGP
                     PVVGQTPNDGY"
     gene            complement(5606..7027)
                     /gene="glnA"
                     /locus_tag="VEO00_04105"
     CDS             complement(5606..7027)
                     /gene="glnA"
                     /locus_tag="VEO00_04105"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HYU57215.1"
                     /translation="MTTPKDVLSMAKDAEAQIVDLRFCDLPGLWQHFSVPISELSEES
                     FQDGFGFDGSSIRGFQEIQESDMLLVPDTSGAFIDPFLKHPTLVLNCFVKDPVTTEWY
                     SRDPRYVAKKAEAYLQSTGIADVSYWGPECEFYIFDSIRFDQNQHSGYYFIDAVEGAW
                     NSGTDEPGGNKGYKPRYKEGYFPVPPMDHYQDLRSEMVINLQRVGIDVEVQHHEVGTA
                     GQSEIDIRYGTLLDTADKVLRYKYVVKNTALRAGKTVTFMPKPIFMDNGSGMHTHQSL
                     WKEGENLFWDEVGYAQISDMCRWYIGGLLSHAPALLAFAAPTTNSYRRLVPGYEAPIN
                     LVYSQRNRSACIRIPVFFKSPGAKRLEFRSPDPTANPYLAFSAMLLAGLDGIKNKTEP
                     PDPIDKDLYDLPPEEAVQVKQVPGSLEEVLDALEDDHGFLLEGGVFTQDVIDTWIEYK
                     RIHELDAVRLRPHPHEFALYFDI"
     gene            complement(7131..7808)
                     /locus_tag="VEO00_04110"
     CDS             complement(7131..7808)
                     /locus_tag="VEO00_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HYU57216.1"
                     /translation="MARIVLLTDRSPDEVLPAIHAVDVDLKAEPLGVDSLVHLPDLSP
                     DWVVVDAEENAWHAYRLLTTLASTDLRMPVVVVVARTDLERFAWAEVADEFLTAGASE
                     VEVRARLAMLRRRVGGGTEATIRLGALAINVETYQVTVAGRPLDLTYKEFELLRFLAE
                     RPGRVFSRAALLQEVWGYDFYGGTRTVDVHVRRLRAKLGPEHEHLIETVRGVGYRAAA
                     VRSTEDG"
BASE COUNT         1074 a         2753 c         2961 g         1183 t
ORIGIN      
        1 tgttgttcga ggcttcggcc ggcaggtcgt actcctcctc gacgcccttc aggcccgcgg
       61 cgagcatggc ggcgaacgcg aggtacgggt tgcaggccgg atcgggggag cggaactcga
      121 tccgtgtggc ctgctccttt cccggcttgt acatcgggac ccgcaccagc gcgctgcggt
      181 ttcgccgcgc ccagcagaca tagacagggg cctcgtagcc gggtaccagg cgcttgtacg
      241 agttcaccca ctggttcgtc acgagcgtga tctctcgggc gtgggtcaag aggccggcga
      301 tgtagccctt ggccgtcttc gacaggtggt actcgtcggt ggcgtcgaag aacgcgttgc
      361 gctccccttc gaacagcgac tggtgggtgt gcatgccgga gccgttgacc ccgtccaccg
      421 gcttcggcat gaacgtggcg tacacgccga actcctgcgc cgcctccttc acggtcagcc
      481 ggtaggtcat cacgttgtcg gccatcgtca gcgcgtcggt gtagcgcagg tcgatctcgt
      541 gctggctcgg cgcgacctcg tgatgcacgt actcgaccgg gatgcccatc gactcgaggt
      601 agaagacggt gcgcttccga tagtcggtgg cgacgtcgag cggcgtcagg tcgaaatacc
      661 cgccgcggtc gaggaactgc gtggtctcgg agtcccggaa gtagaagtac tcgagctccg
      721 ggccgacgaa gaacgtgaat ccgaggtcgg cggcacggtc gagctggcgc ttcaggacga
      781 agcgcgggtc gccgccgaac ggctcgccgt cgggcgtgta gatgtcgcag aacatccgcg
      841 ccacctgcgc ctcggacttc cacgggatga tctggaaggt tgccgggtcg ggacgggcga
      901 ccatgtcgga ctcctggatc cggctgaacc cctcgatcga ggagccgtcg aaccccatcc
      961 cctcggcgaa ggccccctcg agctcgctcg gcgtgatggc gaacgacttc aggaacccca
     1021 ggacgtcggt gaaccacagc cggatgaact tgatcccgcg ttcctcgacc gtccgcagga
     1081 cgtactcggc ctcgttggtg agcgtcatcg caccctcctc gactccgatc cgagcgtacg
     1141 cgcagcccgc cctgagggtg ttacgcgcgc gttaacttcg ccgagttggc gctcagggcc
     1201 gcacggtggg agcctgcccg tcgctggcga gggcggtcac ccagtcgagg atggggacgc
     1261 gcgaaccctc ggagtagaag ctcggcgtct gcagcacgca gtggccgttg ccggcggcga
     1321 tgtaggagct gaagttcggg ccggcggtct cgatggcgtc gaagctcacg cggagcgcgc
     1381 gggagaactg ccgcgggaac ccgccgagca ggaggaagta gagcttctgc gccaggtcgt
     1441 tgtcgtggct gaactcggag aaggtctggt ccgggatcgc gcgcaccagc gcgcggtagt
     1501 agtcggtcat cgtcagccgc ttggggtcga ggtcgcgcac cggcgccagc caggtggcga
     1561 ggtgccgtcc ggcgccgtac ggcgcgtcga ggtcgatcgg cttcgggaag acgccggcga
     1621 acgagtcgcc gagctgcgtg acacgcgcct ccgggtaccg acgggcgacg aacggggcgt
     1681 gcgccgccga tccgatgctc ccggcgctgc atccggccac gaacacctcg gccgggctcg
     1741 ggacgttcgt ggacgcccag cggagcgccg agcgcgcgtt cacccacccg cggtggtaga
     1801 tcgtgaaact gcccccgagc ggggatacgt aggcgacgtc gcggtcaccc cagtgcaggt
     1861 ccccggtgca ctccgggatc accacggccg tgtagtcgcg gaaggggttg cgcgggtcgc
     1921 cgaagtcgag gatgccgttc gccggagggc cgtccggccc gtagcggacg gtgtcccgga
     1981 agaatcgcga gcccggccgg cacgtcgccc cgtcctggca ggcgccgccg ccggtgaaga
     2041 agacgagcag gcgcgacggg tcgccgcggt gtacgaagta ggcgaacggg tctccccgcg
     2101 cgcagagcgt ccggcccccc ggctcgacgc gggtccagcc gtcctgcctg atctggtcga
     2161 acgtgggggt gacgaccgac ggctccggcg ggggtggcgc cggtttcgcc gggcacgccg
     2221 agaacagcac cacccacgcc acggcggccg cggcgcgccg gcgagccgtc gttcccgtcg
     2281 ggcagccgtt catcgcggcg gtcaggctac acgcggctat cgcgttcggg gggagtccgg
     2341 aacccacgcc ggaacgccgg agccgctccg gtagccttgg cccgtgacca cgcccgaggt
     2401 gctgggccgg gccctcgcgg ccgcgcccga cccggagcgc gcccgcgtcg cgctgtcgcg
     2461 ggtgggggag gaccccaggg cccgtgaaac cctcgccgat ccggccgtcc tggccgccgc
     2521 cgtgccccta ctggggctct cgaccgcggt gaccgacttc ctcatggcgc atcccgagga
     2581 ggcaacgctg ttcgcggctc tcgagccgcg cgaccacgcc acgctgatgg aggaagcccg
     2641 ggccgacgcg gctcgcctcg gcgcctcggc ggggctgcgg cggttccgcc gccgcgccct
     2701 cgcgcgggtg gccgccgccg acctggccgg gcggccgttc gagccggtcg tcgccgaggt
     2761 cacggcggtc gccgaggcct gcctgcaggt cgcgctcgag gcggtggacg gggcgggcct
     2821 cgcggtcgtc ggcatgggca agctgggcgg ccgggagctg aactacgcct cggacgtgga
     2881 cgtggtgttc gtccacggcc ggccgggcgg cgagccccag gaggctgcct cccgcgtcgc
     2941 cggcgcgctg ctgcgcctgc tcgccgagcc gaccgatgag ggactggcgc tgcgcgtgga
     3001 cgccgatctc cggccggagg gccgggccgg cgcgctctcg cgatcgctcg ccgcgatggg
     3061 tgagtactac gagcgccacg ccgagacctg ggagcggcag gccatggtca aggcccgccc
     3121 ggtcgccggg gacgccgcgc tcggcgccgc gttcctcgac gccgtcaccc ccctggtgtt
     3181 cccggagcga ctggctccgg cggcgatcga ggacgtccgc cggatgaagg tgcgggtgga
     3241 ggagtacgtg cgcgcccggg ggcgccacga cgtgaagcgt gggccgggcg gcatccgcga
     3301 cgtcgagttc gcggtgcagc tgctgcagct ggtacacggg cggcgtgacc cgtcgctccg
     3361 gacgacgaac acgctcgagg cgctccgcgc gctcgccgag ggggggtacg tggccctggc
     3421 cgacgcggtg gcgctcgcgg actcctaccg cttcctgcgg acgctggagc accgcctgca
     3481 gctgttgcgg gaggtgcagg cccacgacct ccccgaggac gagcagaccc tgcgcaccgt
     3541 ggcgcgagcg atgggcctcg ccggcgccgc cgcgctgcgg aagtcggtcg aagggcacgc
     3601 gggcgtcgtc cgcgccctgc acgagggcct gttctaccgc ccgctgctcg aggccttcgc
     3661 cggcgacgag ccccgccccg gcgtggaccg cgaggcgacg caggagctgc tcgccgggct
     3721 ggggttccgc gaccccgccg gtgcgtacgc ccggctggag gcgatcgtgg acccgtccac
     3781 ccggctgggg agggtgctcg gtacgagctt tccgctgctc ggcccggcgc tggcgctggc
     3841 ggcgaacccc gacgccgcgc tggtgcgctt cgcccgggtc gcggagccgc tgcgcgaggc
     3901 gccgggcctg gccgacaggc tcgcctccca gcccgagacg ctgcaggacc tcgcgtccct
     3961 ggtggccgcc tcgtcggcct tcgccgacgt cgtcgtccgc cggcccgacg tcgtgccggt
     4021 cctgggcccc gcggcttccc cgcccgatcc cgcggtcgat ccggaggccg cgctggtgcg
     4081 ggtcgcggcc cgctacgcct cggggaacct cccggtgccg gagagcgggc gcgcgatcgc
     4141 ggccgtggcc gacgcggtcg tccgccgcgc cgtgcgcgcc ggggctccgg cgctgccgtt
     4201 cgcggcgatc gccctcggca agctggggag cgaggagctg aacttcgcct ccgacctcga
     4261 cctggtgttc gtctacgagg gggaaggggc ggaggcgttc ggcgaggccg cccgggcggc
     4321 ggaggccacg ctggcggcga tccgggccga cgggttcgcg cccgaccccg accttcgacc
     4381 ggaggggcgc gccggcccgc tggtgcgatc ggtggcctcc tacctcgagt actgggagcg
     4441 ctgggccctg acctgggagt tccaggccct gttgaaggcg cgtccggtcg ccggggacga
     4501 ggtgctcggc cggcgcttcc gctcgctggc cgaggacttc gcctacccag aggcgctgcc
     4561 gatcgaccgg gtggccgaga tccgcaagat gcgcgtgcga atggagcagg agcgggtccg
     4621 gccgcgcgag gcggggcggt tccacttcaa gctcggcttc gggggcctcg ccgacgtgca
     4681 gttcgccgtg gagctgtcgc agatgcgcca cggccgggat cacccggccg tccgcgagcg
     4741 tcacaccctc atggcgctcg agcggctggt cgaggcgcgc ctgatcgagg acagcgtggc
     4801 ccgcagcctc ggtgaggcgt atctgttcct gaacgaggtc aagaacgcgc tcgagatcga
     4861 caagcgggtg accgccgagg cggtcccgcc ggcgcccgag gagcaggcgg ccctcgcccg
     4921 gcggctcggc tacgaggagt acccgcgggc gcggttcctg gaggagtacc ggcgcatcac
     4981 ccgccgtgcc aggatggcca tggagcgcgt gttctacggg gaggacgagt gagggtccgc
     5041 ttcggcgcgg cgctcgccct ggccgcgctg acgctcgccg cgtgcggcgg agccggtggg
     5101 aacgccggcg agggtggcat cgagttccgc gagatcggct ccggcggggt gcccgggcag
     5161 gagcggctca cgccggaggc gctcgtgctc cgctccgttg ccgaggcccg cgaccgcctg
     5221 gagggcaggg tggacgctca ggacctggag tccgtggcgt cgctggactt cggcgaccgg
     5281 gccctcgtgg ccgtgttcgc cggcatgggg aacagcccgg cggtcgaggc ccacgtcaag
     5341 gccgttcgcg tccgggaccg ggcgctcgag atcgaggtga acgtgcgcca gcagggcggc
     5401 ccggcgatcg aggctctggt cggtctctat gtgctggtgg acgtcgccgc ggcggacgtc
     5461 gagggcatcg aacgcggaac cgtcgaggtc aagaccgtca agggcccccc cgtcgtcggc
     5521 cagaccccca acgacgggta ctgagcgcct cgaacccgag cgggccgccc gaaggcggcc
     5581 cgctgtccgg ttcgacagaa gcgtcttaga tgtcgaagta caacgcgaat tcgtgcgggt
     5641 gcgggcgcag gcggacggcg tcgagctcgt ggatccgctt gtactcgatc caggtgtcga
     5701 tgacgtcctg agtgaacacg ccgccctcca gcaggaagcc gtggtcgtcc tcgagcgcgt
     5761 ccaggacttc ctcgagcgag cccggtacct gcttgacctg gaccgcctcc tcgggcggca
     5821 ggtcgtacag gtccttgtcg atcgggtcgg gcggctcggt cttgttcttg atcccgtcga
     5881 ggccggcgag cagcatcgcc gagaacgcca ggtaggggtt ggccgtcgga tccggcgaac
     5941 ggaactcgag ccgcttcgcg cccggcgact tgaagaacac cgggatgcgg atgcaggcgc
     6001 tgcggttgcg ctgcgagtac accaggttga tcggggcctc gtagcccggg acgagccgcc
     6061 gataggagtt cgtcgtcggt gcggcgaacg ccagcagcgc cggcgcgtgc gacagcaggc
     6121 ccccgatgta ccagcggcac atgtccgaga tctgcgcgta gccgacctcg tcccagaaga
     6181 ggttctcgcc ctccttccac aggctctggt gggtatgcat gccggacccg ttgtccatga
     6241 agatcggctt cggcatgaac gtgacggtct tgccggcgcg cagcgccgtg ttcttgacca
     6301 cgtacttgta gcgcagcacc ttgtcggcgg tgtcgagcag cgtcccgtag cggatgtcga
     6361 tctcggactg gccggccgtc ccgacctcat ggtgctgcac ctcgacgtcg atcccgaccc
     6421 tctgcaggtt gatgaccatc tcggagcgca ggtcctggta gtggtccatc ggcgggaccg
     6481 ggaagtaccc ctccttgtac cgcggcttgt accccttgtt gccgccgggc tcgtccgtgc
     6541 cggagttcca cgctccctcg accgcgtcaa tgaagtagta gcccgagtgc tggttctggt
     6601 cgaagcggat cgagtcgaag atgtagaact cgcactccgg cccccagtag ctgacatcgg
     6661 cgatcccggt cgactgcagg taggcctcgg ccttcttggc gacgtagcgc gggtcgcggc
     6721 tgtaccactc cgtcgtcacc gggtccttca cgaagcagtt cagcaccagc gtcggatgct
     6781 tcaggaacgg gtcgatgaac gccccgctcg tgtccgggac cagcagcatg tcggattcct
     6841 ggatctcctg gaaccctcgg atcgaggacc cgtcgaagcc gaagccgtcc tggaaggact
     6901 cctcggagag ctccgagatc gggaccgaga agtgctgcca gaggccgggc aggtcgcaga
     6961 accgcaggtc aacgatctga gcctcggcgt ccttcgccat cgacaggacg tccttgggcg
     7021 tcgtcatgcc gccgctccct tctggtcggt tgggcggcag gctagcgagc cgtcgttgcc
     7081 cgatggtttc gcccgggtta actccgtgtt acgtggggtc cgggggaggc tcagccgtcc
     7141 tcggtcgacc gcaccgcggc cgcccggtag cccacacccc ggaccgtctc gatcaggtgc
     7201 tcgtgctcgg ggccgagctt cgcccgcagc cgccggacgt ggacgtccac cgtgcgggtg
     7261 cctccgtaga agtcgtagcc ccacacctcc tgcagcaggg cggctcggga gaagacccgg
     7321 cccgggcgct ccgccaggaa gcggagcagc tcgaactcct tgtaggtcag gtccagcggc
     7381 cggccggcca ccgtgacctg gtaggtctcg acgttgatcg ccagcgctcc cagccggatg
     7441 gtggcctcgg tcccgccgcc gacgcggcgg cggagcatgg ccaggcgggc gcggacctcc
     7501 acctccgagg cgccggcggt gaggaactcg tccgccacct cggcccaggc gaaccgctcg
     7561 aggtctgtcc gggccaccac gaccaccacg ggcatccgca ggtcggtgga ggccagcgtc
     7621 gtcagcagtc ggtacgcgtg ccaggcgttc tcctcggcgt ccaccacgac ccagtcgggc
     7681 gacaggtccg ggaggtggac gagcgagtcc acgccgagag gctcggcctt caggtccacg
     7741 tccacggcgt ggatcgcggg cagcacctcg tcgggagaac ggtcggtgag gaggacgatc
     7801 ctcgccactg gttgaacctc gctcggcggc cggcggccgc gagaagcgag cgtagcgtac
     7861 cggggacccg gcgaacgcgc ggtacgctcg atggccatgg tctccgcgct cgcgccgctg
     7921 tcgcgccggt tccgggtccc ggtcgcgccg ttccggatct cggccgagga c
//
LOCUS       DASPWO010000182         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_27780, whole genome shotgun sequence.
ACCESSION   DASPWO010000182 DASPWO010000000
VERSION     DASPWO010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27780"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..251)
                     /locus_tag="VEO00_04115"
     CDS             complement(45..251)
                     /locus_tag="VEO00_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57217.1"
                     /translation="MTERAQRVGVVVLVLTAVALVFVASAVRSYVPLFFVWVPQFAIP
                     VFLSRAGRKARTEQDAGAEPPADS"
     gene            complement(248..1282)
                     /locus_tag="VEO00_04120"
     CDS             complement(248..1282)
                     /locus_tag="VEO00_04120"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HYU57218.1"
                     /translation="MTGLLASISWPVLDRVHFGGSFAISPHGVGIAFGYLLGSWWVLR
                     EGPKRGLKEEHASSILFWALIGTIFGARFFYVVGHFSEFNSFTDMLKIYNGGISLIGG
                     IAGAIAFAYPVMRRHNYRFLQVMDSAAIGLPFGIFVGRIGDLVIGDHLGKPTSVPWAF
                     VYDGGRLSGFTCTGRVCRETLLPGVAFHPTLTITPHRATLIDPLHGGAIQTGVGVHQT
                     AMYDFLIAATLFLFIYLVMARRPRREGVLIMTFAIWYGTGRVITDFLRVDKRYFGLTG
                     SQWSTAAIAALCTVVLVRWAIQARRRPPPAADGGGGGDEKENLGGAKAAAATTTFTPP
                     VEPGGRGGPT"
     gene            complement(1326..1940)
                     /locus_tag="VEO00_04125"
     CDS             complement(1326..1940)
                     /locus_tag="VEO00_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYU57219.1"
                     /translation="MKADAELLQRGGALVAAAVLIERASSSTPGRTIDRRLFEVVNSE
                     RGPGPDRFFAGLTELGSITASIAAAGVLAATGRHRTAARALGSAAATWFVGQGLKRLY
                     LRARPYDTFTVRLLIGKPRGTSWPSSHPAVLYSFLTVAGRELDLSRAARAALAGIAGL
                     VATSRVYLGVHYPSDVVAGMMLGRGIGLCGVAIGNGSRAGRRGA"
BASE COUNT          362 a          798 c          739 g          262 t
ORIGIN      
        1 tgactaggga caaccgaccg ggccgccggg ttcccgggtg gggatcagga atccgcgggg
       61 ggctccgccc ccgcgtcctg ctcggtccga gccttccgtc cggcacggga caggaacacc
      121 gggatggcga actgcggcac ccacacaaag aacagcggca cgtaggaccg cacggcggac
      181 gccacgaaca ccagcgccac ggccgtgagc accagcacca cgacccccac ccgctgggcg
      241 cgctcggtca cgtcgggcct ccccgcccgc cgggctcgac cggcggtgtg aacgtggtcg
      301 tcgctgcggc agccttcgcc ccgccgaggt tctctttctc gtctccgccc ccgcccccgt
      361 ccgcggcggg cggcggacgt cgtcgggcct ggatggccca ccgcaccagc acgacggtgc
      421 agagcgcggc gatcgcggcc gtgctccact ggcttcccgt gaggccgaag taccgcttgt
      481 ccacccgcag gaagtccgtg atcacccggc ccgtcccgta ccagatggcg aacgtcatga
      541 tcagcacgcc ctcgcgccgc ggccttctcg ccatcaccag gtagatgaac aggaacaggg
      601 tcgcggcgat gaggaagtcg tacatcgcgg tctggtggac cccaacgccg gtctggatcg
      661 cgccaccatg aagtgggtcg atgagtgtgg ctctgtgggg ggtgatcgtc agagtcgggt
      721 ggaacgcgac ccccgggaga agggtctccc tgcacacgcg cccggtgcag gtgaacccgg
      781 acaggcgccc gccgtcgtac acgaacgccc acggcacgct ggtcggcttc ccgaggtgat
      841 cgccgatcac caggtccccg atccgcccca cgaagatccc gaacggcagc ccgatcgcgg
      901 cgctgtccat gacctggagg aaccggtagt tgtggcgccg catgacgggg taggcgaagg
      961 cgatggcccc cgcgatcccg ccgatgaggc tgatcccgcc gttgtagatc ttcagcatgt
     1021 cggtgaagga gttgaactcg gagaagtgcc ccaccacgta gaagaaccgg gctccgaaga
     1081 tggtcccgat cagggcccag aacaggatcg agctggcgtg ctcctccttc agcccccgct
     1141 tcgggccctc gcggagcacc caccacgatc cgagcaggta cccgaaggcg atccccaccc
     1201 cgtggggaga gatggcgaag ctgccgccga aatggacccg gtcgaggacg ggccacgaga
     1261 tggacgcgag caggccggtc acgggggtaa ctgtaacgtc cggccccgca tcgcgagccc
     1321 gaggatcagg ccccgcgacg cccggcacgt gacccgttcc cgatggccac gccgcacagg
     1381 ccgatccccc ggccgagcat catccccgcg accacgtccg agggatagtg caccccgaga
     1441 tacacgcgcg acgtcgccac caggcccgcg atcccggcaa gcgcggcccg ggccgcgcgc
     1501 gacaggtcga gctcacggcc ggccacggtg aggaacgagt acagcaccgc ggggtgcgag
     1561 ctcggccacg acgtcccccg gggcttcccg atcagcaggc gaaccgtgaa cgtgtcgtac
     1621 ggccgcgccc gcaggtacag gcgcttcagc ccctgcccga cgaaccacgt ggcggcggcc
     1681 gaccccagcg ccctcgccgc cgtccggtgg cgcccggtcg ccgccagcac gcccgcggcg
     1741 gcgatcgacg cggtgatcga cccgagctcc gtgagcccgg cgaagaaccg gtcggggccg
     1801 gggccccgtt ccgagttcac cacctcgaac agccgccggt cgatcgtccg gccgggggtc
     1861 gacgacgacg cccgctcgat caggacagcg gcggccacga gcgcgccgcc gcgctggagc
     1921 agctcggcgt cggccttcac ctcggcgccc cctggagcgg cggacgggtg tgcgacaatc
     1981 ccttgcgagc accgcccgac gaagggacac catgcgacga gagggcgacc acgagccgat
     2041 cggggccgac gagccgggtg tccagcagga tcgcgaccac taccgggagc tccagaagca
     2101 gcgccagcgc cgcgtggcca agctgctcgt ggcgctgttc atcacggtcg tgctcatcgt
     2161 g
//
LOCUS       DASPWO010000183         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_876425, whole genome shotgun sequence.
ACCESSION   DASPWO010000183 DASPWO010000000
VERSION     DASPWO010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876425"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2106
                     /locus_tag="VEO00_04130"
     CDS             <1..2106
                     /locus_tag="VEO00_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HYU57220.1"
                     /translation="EHIARRREIRSLTWKFALAAAVAGISLLGSMLLMADHPDDTFKQ
                     VDLLGRLLLPAALRLNDALAGRLALDPQWLKLGLALLTLPVVLWSGQQFYRGTWSGLK
                     HRTADMNTLIGVGTGAAYVYSLVATLAPRVFTRAGLPADVYYEAVAAILALILLGRLL
                     EARAKGRTSEAIRRLEGLRAKTAHVVRHGQETAVPVEEVVRGDLVIVRPGERIPADGV
                     VIEGASAVDESMLTGEPSPAAKKVGDEVIGATINTTGSITFRATRVGKDSALGQIVQL
                     VEDAQTTKAPIQRLADRVAGVFVPVVMALAIAAFVVWFDFGPQPALLFSTVALVTVLI
                     IACPCALGLATPTAILVATGKAAEHGILIRSGEALERLSRVRTVLLDKTGTITEGQPS
                     VTHIVTATRPDGTPISPADVLRWVASVERRSEHPLALAILKAAKDRQVELLAVDKFAV
                     MEGRGVRGTVDRRIIEVISLRHARERSLDLGTLGPDADRLAAQGRIPVIAVVNNTVFA
                     VIAISDPIKPTAQAAILHLRKMGLAVVMVSGDTKKGAEAVAQEVGIDEVVAEVLPSQK
                     ADLVRKLQKQGGRVAMVGDGINDAPALAQADVGIAIGTGTDIAMEASDVTLIRGDPRG
                     VVTAIQLARRTMRTIRWNLVWAFGYNVVLIPVAAGALYPFTGWLLSPVLASAAMAWSS
                     LSVVLNSLTLRRFRPAWAA"
     gene            2114..2404
                     /locus_tag="VEO00_04135"
     CDS             2114..2404
                     /locus_tag="VEO00_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57221.1"
                     /translation="MKYFHRTALAPDQVLEAAKAFFGSRMTPAQEGPQRRAYTSPLGK
                     VTLTVRAEGGHYTLIEAGTDQVGESELDRLAKRFLAEIHKRVEPTHELRGAY"
     gene            complement(2401..>2587)
                     /locus_tag="VEO00_04140"
     CDS             complement(2401..>2587)
                     /locus_tag="VEO00_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HYU57222.1"
                     /translation="MTCGHCQMKVENALKQTFGVYSAIVDLQAGEAEIDFNDDTTTTQ
                     QLVGAVERAGYGARLAG"
BASE COUNT          379 a          889 c          935 g          384 t
ORIGIN      
        1 gagcacatcg cgcgccggcg ggagatccgg agcctcacct ggaagttcgc gctggccgcg
       61 gcggtggcgg ggatctcgct gctcggctcg atgctgctga tggcggatca cccggacgac
      121 accttcaagc aggtggacct gctcggccgc ctgctcctcc cggcggcgct gcggctcaac
      181 gacgccctcg ccgggcggct cgccctcgac ccgcaatggc tgaagctcgg gctcgcgctc
      241 ctcaccctgc cggtggtgct gtggtcggga cagcagttct accggggcac atggagcggg
      301 ctcaagcacc ggaccgccga catgaacacc ctgatcggcg tggggacggg ggccgcctac
      361 gtctactccc tcgtcgcgac cctcgccccc cgcgtcttca cccgcgccgg cctgccggcc
      421 gatgtctact acgaagccgt cgctgcgatc cttgcgctga tcctgctcgg ccggctgctc
      481 gaggcgcgcg ccaagggccg gacctcggaa gcgatccgcc ggctcgaggg gctgcgggcc
      541 aagaccgccc acgtggtccg gcacggccag gagaccgccg tgccggtgga ggaggtggtg
      601 cggggcgact tggtgatcgt gcggccgggg gagaggatcc cggcggatgg cgtcgtgatc
      661 gaaggggcgt cggccgtgga cgagtcgatg ctcaccggcg aacccagccc cgcggcgaag
      721 aaggtcggcg acgaggtgat cggcgcgacg atcaacacca cggggagcat caccttccgc
      781 gccacgcgcg tggggaagga ctcggcgctc ggccagatcg tccagctcgt ggaggacgcg
      841 cagaccacca aggcgccgat ccagcggctc gcggaccggg tggccggggt gttcgtaccc
      901 gtcgtcatgg cgctggcgat cgccgcgttc gtggtctggt tcgacttcgg tccccagccg
      961 gcgctgctct tctcgaccgt cgccctcgtc accgtgctga tcatcgcgtg cccctgcgcc
     1021 ctcggcctgg cgacgccgac cgcgatcctc gtcgcgaccg gcaaggccgc cgagcacggg
     1081 atcctgatcc ggagcggcga ggccctggaa cggttgtcgc gggtgcgaac ggtgctgctc
     1141 gacaagaccg gcacgatcac cgaaggtcag cccagcgtca cccacatcgt caccgcgacg
     1201 cggccggacg gcacgccgat ctcccccgcg gacgtgctca ggtgggtggc ctcggtggag
     1261 cggcgctcgg agcacccgct ggcgctcgcg atcctcaagg cggccaagga ccggcaggtc
     1321 gagctcctcg cggtcgacaa gttcgccgtg atggagggcc gcggtgtgcg cgggacggtg
     1381 gaccgacgca tcatcgaggt gatctcgctg cggcacgcgc gggagcgcag cctcgacctc
     1441 ggcaccttgg gtccggacgc cgaccggctc gcggcgcagg gccgcatccc ggtgatcgcg
     1501 gtcgtgaaca acacggtgtt cgcagtcatc gcgatctcgg acccgatcaa gccaaccgca
     1561 caggccgcga tcctccacct caggaagatg ggccttgcgg tcgtgatggt ctccggcgac
     1621 acgaagaagg gcgcggaggc ggtcgcgcag gaagtgggga tcgacgaggt cgtcgccgaa
     1681 gtgctcccgt ctcagaaggc cgacctggtg aggaagctcc agaagcaggg cggccgcgtg
     1741 gcgatggtcg gcgacgggat caacgacgcc cccgcgctgg cgcaggcgga cgtcgggatc
     1801 gcgatcggca ccggcacgga catcgcgatg gaggcgtcgg acgtcacgct catccgggga
     1861 gacccgcggg gagtggtgac ggcgatccag ctggcgcggc ggacgatgcg gacgattcgc
     1921 tggaatctcg tgtgggcgtt cggctacaac gtcgtcctca tcccggtggc cgcgggcgcg
     1981 ttgtacccgt tcacgggctg gctcttgtcg cctgtgctgg cgagcgcggc catggcgtgg
     2041 tcgagcctct ccgtcgtgtt gaacagcctc acgctgcggc ggttccgtcc ggcgtgggcg
     2101 gcttgaggga tctgtgaagt acttccaccg cacagcactc gccccggacc aggtgctcga
     2161 ggcggccaag gcgttcttcg ggtcccggat gacgcccgcc caggaggggc cgcagcgccg
     2221 cgcgtacacg agtccgctcg ggaaggtgac cctcaccgtg cgggcggagg gggggcacta
     2281 cacgctcatc gaggcgggca ccgaccaggt aggggaatcc gagctcgacc ggctcgcgaa
     2341 gcgattcctg gccgagatac acaagcgagt cgagcccacg cacgagctgc gcggggcgta
     2401 ctagccggcc agcctcgccc cgtaccccgc ccgctccacc gccccgacga gctgctgggt
     2461 ggtcgtcgtg tcgtcgttga agtcgatctc ggcttcgccc gcttgcaaat ccacgatcgc
     2521 ggaatagacg ccgaacgtct gcttcagcgc gttctcgact ttcatctggc agtgcccgca
     2581 cgtcatg
//
LOCUS       DASPWO010000184         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1128870, whole genome shotgun sequence.
ACCESSION   DASPWO010000184 DASPWO010000000
VERSION     DASPWO010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128870"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VEO00_04145"
     CDS             <1..447
                     /locus_tag="VEO00_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HYU57223.1"
                     /translation="GSVWPHDNALIAAGLKRYGFLKATNRVTTAIFDAAIHADYMRLP
                     ELFCGFTRRTPNQPVAYPVANSPQAWAAGAPFLMLQAMLGVSARAHENLLTVNKPQLP
                     PWLNTVELRNLRVGESTITLVFRREGEVTGFSMLQKEGDVRVVMEE"
     gene            516..1076
                     /locus_tag="VEO00_04150"
     CDS             516..1076
                     /locus_tag="VEO00_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57224.1"
                     /translation="MRRRTLLLAIAATLSLVLLASACGRASLREGFGGAPGPTGGNTS
                     SPGDGCVSIPPIPGASRNSDSPVGSPCDTTPGGPPEPIEPTPRLVTPSPRMENVHPIG
                     WDGYEVVSPDGRVLRVTFSSGIEPCAVLDHVTVRETDARVTITLYEGSDPAHPDTACI
                     EIAELKAVDATLDAPVGNREVVDPTD"
     gene            complement(1090..2262)
                     /locus_tag="VEO00_04155"
     CDS             complement(1090..2262)
                     /locus_tag="VEO00_04155"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HYU57225.1"
                     /translation="MNLVDLLIAALAVLAIVSGFRRGAALQILTYVGLLAGLFAGALL
                     APPVAGLVDTPLAQAVAALVVLVALTGAGDALGWWVGARAWTAARRSRLSPVDAAAGS
                     VVSVVALLLATWLLAFNLVQGPFPFLARQIRGSAIVRGLDEILPAPPSILTEARRFLD
                     RFGFPEVFTNLPPAPAGPVALPSDAEARRAAAGAVGSTVRIVGEACGLIQEGSGFVAA
                     DGYIVTNAHVVAGVPAPQVQQQNGGSEGATTVLFDPDLDVAVLRVGTSPGPALTLRRD
                     VVDRGARGAVLGYPGGGPLDVERAAVRRSLEAVGRDIYGRRTVVREVYEVQAAVRPGS
                     SGGPFVLGDGTVAGVVFAASTTEGNVGYALTSTSVGPDVRSALGRTAPVDTRPCIR"
     gene            2339..3100
                     /locus_tag="VEO00_04160"
     CDS             2339..3100
                     /locus_tag="VEO00_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU57226.1"
                     /translation="MTNVAAEERRAPAGTACRAVDAVKVYGSGEAAVRALDGVTVDFE
                     AGRFTAIMGPSGSGKSTLLHCLAGLDDLTSGQVFIGGIDLTTLSEKERTLLRRDRVGF
                     VFQAFNLIPTMTAEENINLPVDLARRDPDRKWVERLVSILGLGDRLDHRPSELSGGQQ
                     QRVAAARALSSRPDIVIADEPTGNLDSHLGAELLSFLEQVVRDFGQTIVMVSHDPVAA
                     GHADRVVFLADGRVAGEMHHPTVDGILDRMKLLET"
     gene            3105..>3604
                     /locus_tag="VEO00_04165"
     CDS             3105..>3604
                     /locus_tag="VEO00_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU57227.1"
                     /translation="MLRAALRSLLAHKVRLGLTALAVVLGVSFIVGTYVLTDTINATF
                     ENLFRDVSSGTDVYVRSEAAFESQFQMTRQPVDEEVLDTVLAVPGVSEADGTVQGFAQ
                     LIDKEGEAIVPSGPPTLGVNWSNTSLNPLTLRSGDPPRSDDQVAIDASTARRFEFGPG
                     DQIRIV"
BASE COUNT          540 a         1327 c         1273 g          464 t
ORIGIN      
        1 ggttcggtct ggccgcacga caacgcgctg atcgcagccg gcctgaagcg ttacggcttc
       61 ctgaaggcga ccaaccgcgt caccaccgcg atcttcgacg cggccatcca cgcggactac
      121 atgcgcttgc cggagctctt ctgtggcttc acccgccgca cgccgaacca gccggtggcc
      181 taccccgtgg cgaactcgcc acaagcctgg gcggccggcg caccgttcct gatgctccag
      241 gccatgctcg gcgtctccgc ccgcgcccac gagaacctcc tgacggtgaa caagccgcag
      301 ctgcccccct ggctgaacac cgtcgagctg cggaaccttc gcgtcggcga atcgacgatc
      361 acgctcgtct tccggcgcga gggcgaggtg accggcttct cgatgctgca gaaggagggc
      421 gacgtccggg tggtgatgga ggagtagccc cgccggggaa cgccggccgc ggcccgcccg
      481 tcgtaccggc ggccgatccg atcggagcga ttcagatgcg acgacggacg ctgctcctgg
      541 cgatcgccgc gaccctctcc ctcgtcctgc tggcttcggc ctgcggacga gcctcgctgc
      601 gcgagggatt cggcggggcg cccgggccca ccggcgggaa cacctcctcc ccgggcgacg
      661 ggtgcgtcag catcccgccg atccccggcg cgtcgaggaa ctccgactcg ccggtcggca
      721 gcccctgcga cacgacgccg ggggggccgc ccgagccgat cgagcccact ccccgcctgg
      781 tcactccgag tccgcggatg gagaacgtgc acccgatcgg ctgggacggc tacgaggtcg
      841 tctcccccga cggccgcgtg ctgcgcgtga ccttctccag cggcatcgag ccctgcgccg
      901 tcctggacca cgtcacggtt cgggagaccg acgcgcgggt gacgatcacc ctgtacgagg
      961 ggagcgaccc ggcgcatccc gacaccgcgt gcatcgagat cgccgagctg aaggccgtcg
     1021 acgcgacgct cgatgcgccc gtcggtaacc gcgaggtcgt cgaccccacc gactgatcgc
     1081 agccgaccgt cagcggatgc aggggcgggt gtcgaccggg gcggtgcggc cgagggcgga
     1141 ccgcacgtcg gggccgacgg aggtggacgt cagcgcgtag ccgacgttcc cctcggtcgt
     1201 cgacgccgcg aagacgacac cggcgaccgt cccgtcaccg aggacgaacg ggcccccgga
     1261 gctccccggc cggaccgccg cctgcacctc gtagacctcg cgcaccacgg tgcgccggcc
     1321 gtagatgtcg cgcccgacgg cttccaggct tcggcgcacc gccgcccgct cgacgtcgag
     1381 cggtccacct cccgggtacc cgagcaccgc gccccgcgcc cctcggtcga cgacgtcccg
     1441 ccggagcgtc agcgccggcc cggggctcgt cccgacgcgc agcacggcca cgtcgagatc
     1501 ggggtcgaag aggacggtcg tcgcgccctc cgacccgccg ttctgctgct gcacctgagg
     1561 cgcgggcacg ccggccacca cgtgcgcgtt cgtcacgatg tacccgtcgg cggcgacgaa
     1621 gccgctgccc tcctggatca acccgcaggc ctcgcccacg atccgcaccg tgctcccgac
     1681 ggcgccggcg gctgcccgcc gagcctccgc gtcgctcggc agcgccaccg gcccggcggg
     1741 ggcgggcggg aggttggtga agacctccgg gaacccgaag cggtccagga agcgccgcgc
     1801 ctcggtcagg atcgaggggg gcgcggggag gatctcgtcg aggccgcgga cgatcgccga
     1861 gcctcggatc tggcgcgcca ggaacgggaa gggcccctgg accaggttga aggcgagcag
     1921 ccaggtggcg agcagcagcg ccacgaccga gaccaccgaa ccggccgcgg cgtccaccgg
     1981 cgagagccgg ctgcgccggg ccgccgtcca ggcccgcgct ccgacccacc accccagagc
     2041 gtcgccggcg ccggtcagcg caacgaggac gacgagggcc gccacggcct gggcgagcgg
     2101 cgtgtccacc agcccggcga ccgggggcgc gagcagcgcc ccggcgaaca gacccgccag
     2161 gagcccgacg taggtgagga tctggagggc cgcaccgcgc cggaaccccg agacgatcgc
     2221 cagcaccgca agcgcggcga tcagcaggtc gaccaggttc acgtcgtggc catttccggc
     2281 gcgtatcctg ccatgcgccc gcgtaccggc ccgcgcgacg tatcgacctg gaggcgagat
     2341 gaccaacgtg gctgccgagg agcgccgcgc cccggcggga acggcgtgca gggccgtgga
     2401 cgcggtcaag gtctacgggt cgggggaggc cgccgtgcgc gcgctcgacg gggtgacggt
     2461 cgacttcgag gccgggaggt tcacggcgat catgggcccg tcgggctcgg ggaagtcgac
     2521 gctcctgcac tgcctcgccg ggctggacga tctgacctcg ggacaggtct tcatcggcgg
     2581 catcgacctg accacgctgt ccgagaagga acgcacgctg ctgcgccgcg atcgcgtggg
     2641 gttcgtcttc caggcgttca acctgatccc gacgatgacc gcggaggaga acatcaacct
     2701 cccggtcgac ctggcccggc gcgaccccga ccggaagtgg gtggagcgcc tggtatcgat
     2761 cctcggcctg ggcgaccggc ttgaccaccg gccgagcgaa ctgtccggcg gccagcagca
     2821 gcgcgtcgcc gccgcgcggg ccctctccag ccgcccggac atcgtcatcg cagacgagcc
     2881 gacgggaaac ctcgattccc atctcggggc ggagctgctg tccttcctcg agcaagtggt
     2941 gcgggacttc ggccagacca tcgtgatggt cagccatgat cccgtggcgg cggggcatgc
     3001 cgaccgcgtc gtattcctgg cagacggccg cgtcgccggg gagatgcacc atccgacggt
     3061 cgacggcatc ctcgatcgga tgaagctcct ggagacgtag cggcgtgctg cgagccgcgc
     3121 tgcggagcct cctggcacac aaggtccggc tgggcctgac cgcgctcgcg gtggtgctcg
     3181 gggtgagttt catcgtcggg acctacgtcc tgaccgacac gatcaacgcc acgttcgaga
     3241 acctgttccg cgacgtcagc tccggaaccg acgtctacgt ccggtcggag gcggcgttcg
     3301 agagccagtt ccagatgacc cgccagccgg tggacgagga ggtgctggac accgtactcg
     3361 ccgtccctgg cgtgtcggag gctgacggca cggtccaggg gttcgcgcag ctcatcgaca
     3421 aggaaggcga ggcgatcgtc ccgtccggtc cgccgaccct cggcgtgaac tggtcgaaca
     3481 cctccttgaa tcccctgacg ctgcgatccg gcgacccacc gcgctccgac gaccaggtgg
     3541 cgatcgacgc gagcacggcc cggaggttcg agttcgggcc gggggaccag atccggatcg
     3601 tctt
//
LOCUS       DASPWO010000185         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_795752, whole genome shotgun sequence.
ACCESSION   DASPWO010000185 DASPWO010000000
VERSION     DASPWO010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795752"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VEO00_04170"
     CDS             complement(<1..248)
                     /locus_tag="VEO00_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HYU57228.1"
                     /translation="MSSAFESVRGFFEEAAETLQLELSLREALATPAREVSVQVRVPM
                     DMGGFRVFKGYRVQYNNARGPYKGGLRYHPIVSLDEVR"
     gene            complement(309..1175)
                     /locus_tag="VEO00_04175"
     CDS             complement(309..1175)
                     /locus_tag="VEO00_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU57229.1"
                     /translation="MARDSATLQRTSAEARAAGPGRKHDLRAVKIVWQRELIRFAQDR
                     PRILVSLLQPVLFLFVLGTGLGSLVSGGAGGVSLRTFLFPGVLGMAVMFTAMFSAASI
                     VWDREFGFLREMLVAPVARGGIVLGKCLGGASVAAFQGVILLALAGTVGVPYSPLLIL
                     TLTGELLLLAFVITALGVLLAARIRSIQSFFSLTQMLLMPLFFLSGALFPLTNLPTWL
                     QVLTHVNPLTYAVDPMRRAVFARLSPGGHPLSTDVTWGSWRVPTLLELALVAALALAL
                     LAVAVVQFRRPD"
     gene            complement(1181..2221)
                     /locus_tag="VEO00_04180"
     CDS             complement(1181..2221)
                     /locus_tag="VEO00_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU57230.1"
                     /translation="MRRVMEERATAPESSAAVATEGDGDAAVVVRGLAKRYGEIEAVA
                     GVDFDVRRGETFGFLGPNGAGKSTTINMLCTLTQPTTGSARVAGHDIVAERDAVRRSI
                     GLVFQETTLDSYLTAERNLRFHAELYGVPRAGLDERLREVLEMVGLWDRRGSRVMTFS
                     GGMMRRLEIARGLLHTPRVLFLDEPTIGLDPQTRASIWRYIQDLKQREDITIFMTTHY
                     MDEAEYCDRIAIIDSGRIVALDTPQALKASVGKDRVQIQTPDDDAAISALRQRFGIEA
                     AVHEGAVTFAVPEGEQFVPRLFAELGVQIGSVSVSRPTLDDVFLAYTGTTIRDAEGVD
                     PNRAIVAAFRRR"
BASE COUNT          381 a          855 c          853 g          322 t
ORIGIN      
        1 cagcgcacct cgtcgagcga gacgatcggg tggtagcgca gcccgccctt gtacggaccg
       61 cgggcgttgt tgtactggac gcggtagccc ttgaacaccc ggaagccccc catgtccatc
      121 ggcacgcgga cctggacgga cacctcgcgg gccggcgtgg cgagggcctc gcggagggac
      181 agctcgagct gcagcgtctc ggcggcttcc tcgaagaagc cgcggaccga ctcgaaggcc
      241 gagctcatga cgtcatcgtc ctgaaccggg ctatcctacc ccccggcggc ccccggagga
      301 aacgatggtc agtcggggcg gcggaactgg acgaccgcca cggcgagcag cgccagggcc
      361 agcgcggcga ccagggccag ctcgaggagc gtgggcaccc gccagcttcc ccaggtcacg
      421 tcggtggaca ggggatgccc gccggggctg aggcgggcga acacggcacg gcgcatcggg
      481 tcgacggcgt acgtcagcgg gttgacgtgg gtcaggacct gcagccaggt cgggaggttc
      541 gtcaggggga acagcgcccc cgacaggaag aacagcggca tcagcagcat ctgcgtcaac
      601 gagaagaacg actggatgct gcggatgcgg gcggcgagga gcacgccgag cgcggtgatc
      661 acgaacgcca gcaggagcag ctcgccggtg agcgtcagga tcagcagggg ggagtaggga
      721 acccccaccg tccccgccag agcgagcagg atcacgccct ggaaggccgc gaccgaggcg
      781 ccccccaggc acttcccgag cacgatcccg ccgcgggcga ccggcgccac cagcatctcc
      841 cgcaggaaac cgaattcccg gtcccagact atcgaggccg ccgagaacat cgccgtgaac
      901 atgacggcca tccccaggac tcccgggaac aggaacgtcc gcaggctcac gccgcccgcg
      961 cctcccgaga cgagcgaccc gagcccggtg ccgagcacga acaggaagag caccggctga
     1021 agcaacgaca cgaggatgcg cggccggtcc tgcgcgaacc ggatcagctc gcgctgccag
     1081 acgatcttca ccgcgcgcag gtcgtgcttc cggccgggcc ctgccgcccg ggcctctgcg
     1141 gacgtgcgct gcagcgtggc cgaatcccgc gccaccccga tcaccgcctc cggaacgcgg
     1201 ccacgatggc gcggttcggg tcgacgccct cggcgtcgcg gatcgtcgtt cccgtgtagg
     1261 caaggaagac atcgtcgagg gtcggacggg agacgctcac cgagccgatc tgcaccccga
     1321 gctccgcgaa caggcggggg acgaactgct cgccctccgg gacggcgaag gtcaccgccc
     1381 cctcgtgcac cgcggcctcg atgccgaagc gctgtcgcag cgcggagatg gccgcgtcgt
     1441 cgtcgggggt ctggatctgg acgcggtcct tgccgacgct cgccttcagc gcctgcggcg
     1501 tgtcgagcgc gacgatccgc ccgctgtcga tgatcgcgat gcggtcgcag tactcggcct
     1561 cgtccatgta gtgcgtggtc atgaagatcg tgatgtcctc tcgctgcttg aggtcctgga
     1621 tgtagcgcca gatcgacgcg cgcgtctgag ggtcgagccc gatcgtcggc tcgtcgagga
     1681 acaggacccg cggtgtgtgc agcagcccgc gggcgatctc cagccggcgc atcatgccgc
     1741 cggagaaggt catcacgcgg ctgccgcgcc gatcccacag cccgaccatc tccagcacct
     1801 cgcgcaggcg ctcgtcgagt ccggcgcggg ggaccccgta gagctccgcg tggaaacgca
     1861 ggttgcgctc ggcggtgagg tacgagtcga gggtggtctc ctggaacacg agtccgatgc
     1921 tgcgccggac ggcgtcgcgc tcggcgacga tgtcgtgccc ggccacgcgc gcggaccccg
     1981 tggtcggctg cgtcagcgtg cagagcatgt tgatcgtggt ggacttcccg gcgccgttcg
     2041 gcccgaggaa cccgaacgtc tcgccgcgcc ggacgtcgaa gtcgactccg gcgaccgcct
     2101 cgatctcgcc gtagcgcttg gccaggccgc ggacaaccac ggccgcgtcg ccgtcgccct
     2161 cggtggcgac cgcggcgctc gactccggcg cggtggcccg ttcctccatc acccgccgca
     2221 cgctcccttc cgctccagtc tctccaccgt accggctctc gcccgtttcg aggcgagggg
     2281 ctactgtcgg ggcgtggtcc ggatcctggc ggtggccgac gaggtcgacg agtcgctcgg
     2341 ccgggagacg atgggatcgc tccggcccga cgtggtcgtc tcgtgcgggg acctcccctt
     2401 cgaccacctg g
//
LOCUS       DASPWO010000186         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_450215, whole genome shotgun sequence.
ACCESSION   DASPWO010000186 DASPWO010000000
VERSION     DASPWO010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450215"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..>2027
                     /locus_tag="VEO00_04185"
     CDS             108..>2027
                     /locus_tag="VEO00_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYU57231.1"
                     /translation="MRAVVMAGGQGSRLRPLTSNQPKPMLPIVGRPMLEHILRLVRRH
                     GMVDVVATVQFLGSIIRRAFGDGSDLGLSIRYAIEEEPLGTAGSVKNAEELLDETFLV
                     ISGDALTDIDLTDAVSFHRRRGAAATVVLRRVEDPLEFGIVIVGDDGRVERFLEKPGW
                     GEVFSDTINTGIYVLEPEVLRFVPDGEEFDFAQDLFPVLLDKGLPMYGYAAEGYWTDV
                     GNLEAYQAAHRDVLDGRVDLELEGFSLGEGVWLGDGAEIDPEANVSGPAFIGEGSRVE
                     AGATILEYSVVGRNVVVKPGAFLHRAIVHDNAYIGPSVSLRGCVVGRNADVKHGARLE
                     EGVVVSDDCMVGEGAVLNPQVKVYPFKRVDPGALVSQSIIWESRGARSLFGERGVAGL
                     VNVDVTPEMAVRLGLAFGSILPRRSVVTACRDTSRAARIVKRAMVAGLNAAGVNVHDL
                     ELIPSAVARFYARSARAVGGIAVRTSPYDPAGVEVQFFDGNGVDVDQVTARKLERTFA
                     RDDFRRAYHHEIGELTFPARGREYYARGLVDAVDADVVRESGPKLVVDYLFGSAALTA
                     PGILGRVGADVLAVNAVLDEERLPTSEGDLGAHLETVARLVTASRADLGAVIDPPGER
                     LHLVDDRGRIADPAVA"
BASE COUNT          274 a          689 c          769 g          295 t
ORIGIN      
        1 agtactgggc cgcggcggtg gcgtacctga tcgagcttcg ccgggccgtc cgggcgcggc
       61 ggggggccgg gggttagccc gaaccaccgc ggatatcatc cttgccgatg cgtgccgtgg
      121 tcatggcggg gggccagggc agccgtctcc gccccctgac cagcaaccag ccgaagccga
      181 tgctcccgat cgtcggccgt ccgatgctgg agcacatcct gcggctggtc cgccgccacg
      241 ggatggtcga cgtggtggcc acggtgcagt tcctcgggag catcattcgc cgcgccttcg
      301 gggacggctc ggacctgggg ctctcgatcc gctacgcgat cgaggaggaa ccgctcggta
      361 cggccggaag cgtgaagaac gccgaggagc tgctcgacga gacgttcctc gtcatctccg
      421 gcgacgcgct gaccgacatc gacctgactg acgcggtgtc cttccatcgc cgccgcgggg
      481 ccgcggccac cgtggtcctg cggcgcgtgg aggacccgct cgagttcggg atcgtcatcg
      541 tcggcgacga cggccgcgtg gagcggttcc tggagaagcc gggctggggc gaggtcttca
      601 gcgacacgat caacacgggc atctacgtcc tggagccgga ggtcctgcgc ttcgtccccg
      661 acggcgagga gttcgacttc gcccaggacc tgttccccgt gctgctcgac aagggactcc
      721 cgatgtacgg ctacgccgcc gaggggtact ggaccgacgt cgggaacctg gaggcgtacc
      781 aggcggcgca ccgcgacgtg ctcgacgggc gggtcgacct ggagctcgag gggttctcgc
      841 tcggggaggg ggtctggctg ggcgacggcg ccgagatcga ccccgaggcg aacgtctccg
      901 gcccggcgtt catcggcgag ggctcccgcg tcgaggccgg ggcgacgatc ctggagtaca
      961 gcgtggtggg gcgcaacgtc gtggtgaaac cgggggcgtt cctgcatcgg gccatcgtcc
     1021 acgacaacgc ctacatcgga ccgtccgtat cgctgcgagg ctgcgtggtg gggcgcaacg
     1081 ccgacgtgaa gcacggcgcc cggctcgagg agggcgtggt cgtctccgac gactgcatgg
     1141 tcggcgaggg cgccgtcctg aaccctcagg taaaggtcta ccccttcaag cgggtcgacc
     1201 ccggcgcgct cgtctcgcag agcatcatct gggagtcgag gggggcgcgg tccctgttcg
     1261 gcgagcgcgg ggttgccggc ctcgtgaacg tcgacgtcac gccggagatg gccgtgcggc
     1321 tggggctcgc cttcgggagc atcctgccgc ggcgttcggt ggtcacggcc tgccgggaca
     1381 cgtcgcgcgc ggcgcggatc gtcaagcgcg ccatggtcgc cggcctgaac gccgcagggg
     1441 tgaacgtcca cgacctggag ctgatcccct cggccgtggc acgcttctac gcccgatcgg
     1501 cgcgggcggt gggcgggatc gcggtgcgca cctccccgta cgacccggcc ggggtggagg
     1561 tccagttctt cgacgggaac ggcgtcgacg tcgaccaggt cacggcccgc aagctcgagc
     1621 ggacgttcgc gcgcgacgac ttccgccgcg cctaccacca cgagattggc gagctgacgt
     1681 tcccggcgcg cggacgggag tactacgcgc ggggcctggt ggacgcggtc gacgccgacg
     1741 tggtccgcga gtccggcccg aagctcgtcg tcgactacct gttcggcagc gccgcgctga
     1801 ccgcgcccgg catcctcggg cgggtcgggg cggacgtcct ggcggtgaac gcggtcctcg
     1861 acgaggagcg cctgccgacg tcggagggag acctcggcgc gcacctggag accgtcgccc
     1921 ggctggtcac cgcgagccga gccgacctcg gggcggtgat cgacccgccg ggggaacgcc
     1981 tccacctcgt cgacgaccgg ggccggatcg ccgaccccgc ggtggcg
//
LOCUS       DASPWO010000187         4869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_876940, whole genome shotgun sequence.
ACCESSION   DASPWO010000187 DASPWO010000000
VERSION     DASPWO010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876940"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VEO00_04190"
     CDS             <1..229
                     /locus_tag="VEO00_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57232.1"
                     /translation="LPSGGSSISIIGIEAATGNPADSPTGDGIVPYVALVPIGVTADN
                     MADTVIADGFRTIEEICTGDVAETDFCQENA"
     gene            326..1108
                     /locus_tag="VEO00_04195"
     CDS             326..1108
                     /locus_tag="VEO00_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU57233.1"
                     /translation="MADTALLECRGISKAFGAVRALYKVDFEVRRGEVMALVGDNGAG
                     KSTLIKGIAGIYPFDEGEVLFEDAPVTIHGPREAARLGIEVVYQDLALADNLDVVANM
                     FLGRERTRGGYVLDESSMERSCEETLRGLSVTTLRSVRQTVVGLSGGQRQAVAVAKSV
                     MWNSKLVILDEPTAALGVAQTRQVLDLVRRLAEKDLGVVIISHNLHDVFEVADRITVL
                     RLGQQVGVYRREATNQQEVVHAITAGTLAHVPGMADARVAER"
     gene            1105..2400
                     /locus_tag="VEO00_04200"
     CDS             1105..2400
                     /locus_tag="VEO00_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU57234.1"
                     /translation="MTTEPDTSSGAEPVVRGPEEEVLPPAAPPVEVPAETLGGYARRW
                     WEKIRAGDLGSLPLVVGLVVIVAVFGGLDSTFFVERNFTNLLLQMAPLATIAIGIVFI
                     LLIGEIDLSVAYVSAVGGVMMTLLLRPEASNWPWWAAIGVAVAITTAIGFVHALVVTK
                     AGVPSFVVTLAGLLIWSGVVLILTTTYSTAGTIRIQNPTVIGLANNFLTHTWGWIVAI
                     GVVAGYALTQWQTGRARRAGGLYAKPVSLLALQVAGLAAVTFAAVWYANRDRGVPTVT
                     LILLALLVFWSFIAARTRFGRHVYAVGGNPEAARRAGIGVDRVRIMVFMINGFMAGVG
                     GILFASRLRSVATGTGGGNLLLSVIAAAVIGGTSLFGGTGRVVAALLGALMIASIQNG
                     MGLLNLASGPQFVITGGVLLAAVLIDSLSKRRRAARGVL"
     gene            2428..3591
                     /gene="xylA"
                     /locus_tag="VEO00_04205"
     CDS             2428..3591
                     /gene="xylA"
                     /locus_tag="VEO00_04205"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HYU57235.1"
                     /translation="MSYEPRPEHRFSFGLWTVGHPGFDPFGDPVREVLSPVQLVELLA
                     EVGAWGVNFHDNDLVPIDASAHERDQIVSDFRKALDATGIVVPMATTNLFRDPVFRDG
                     AFTAHDPRVRAFALQKTMRAIDLGVELGATTYVFWGGREGVETDAAKDPLVAFERYRE
                     GMNFLCAYVRDRGYDLHFALESKPNEPRGDIYLSTTGAMLAFIETLDHAEMVGVNPEV
                     AHEHMAGLNSMHHVAQAWAMGKLFHVDLNDQIPGRYDQDLRFGSGNPKAAFFLVKLLG
                     DVGYAGSRHFDAHAYRTSDYEDVKAFARGCMRTYLILEEKAERWNADPDIQALVAEIT
                     ADDGAMAEVLGPYTPERAAALSRLALDRRALGARGQPYERLDQLTMDLLLGVR"
     gene            3588..4400
                     /locus_tag="VEO00_04210"
     CDS             3588..4400
                     /locus_tag="VEO00_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57236.1"
                     /translation="MTLETVRVEGYECARLDDGNSALTITVSTGPRVLELRARGENLL
                     ALVPDATVPVSGGGRLRLLGGHRLWVAPEVPAITYQPDDRPCQAIEVEDGVRVTAPPD
                     GAGLTKSLEVRRAADGWNVDHEIRNDSHEAMELAPWAVTMLRPGGEAVLAAAAGGEGL
                     QADRAIVLWPYTDPADARVSIERDALRVRCAPGPTLKLGVASLDGRATYSLNGETFEK
                     RTVVERGARYADRGAAVQLYVSDAFCEVETLGPLATVEPGGAVAHRERWRLW"
     gene            4397..>4869
                     /locus_tag="VEO00_04215"
     CDS             4397..>4869
                     /locus_tag="VEO00_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HYU57237.1"
                     /translation="MSHVLGLDVSTTATKAVVVDRGGEVRAAAAADYPYETPRPLWSE
                     QEPARWWEATVAAVQRALGEAGIRGADVEAVGLAGQMHGLVLLDEAGAVLRPAILWND
                     QRTGAECDLIREAVGAEALIAITGNDALPGFTAPKLLWVRRHEPEIWSRIAHVLLP"
BASE COUNT          666 a         1682 c         1807 g          714 t
ORIGIN      
        1 gctgccatcg ggcggttcgt cgatctcgat catcgggatc gaggcggcga cagggaaccc
       61 cgccgacagc cccaccgggg acggcatcgt cccctacgtg gccctggtgc cgatcggggt
      121 gaccgcggac aacatggccg acaccgtgat cgccgacggg ttccgcacga tcgaggagat
      181 ctgcacgggc gacgtggccg aaacggactt ctgccaggag aacgcctagc gctacggtcc
      241 ggaggcagga accgcgccat ggacggcggg gacgctccga gcgggctccc cgccgccgtg
      301 gccgctctcc gtgagaggat gggtcgtggc tgatacggcc ctcctcgagt gcaggggcat
      361 ctcgaaggcg ttcggcgcgg tgcgggcgct ctacaaggtc gacttcgagg tgcgtcgcgg
      421 cgaggtgatg gccctcgtcg gcgacaacgg cgccgggaag tccacgctga tcaagggcat
      481 cgccgggatc taccccttcg acgagggcga ggtcctcttc gaggatgcgc cggtcacgat
      541 ccacgggccg cgcgaggcgg cgcggctcgg catcgaggtc gtgtaccagg acctcgccct
      601 ggcggacaac ctcgacgtgg tggcgaacat gttcctcggc cgggagcgga cccgcggcgg
      661 ctacgttctc gacgagtcga gcatggagcg ctcctgcgag gagaccctgc ggggcctgtc
      721 ggtgacgacg ctgcggtcgg tccgccagac cgtggtcggc ctgtccgggg gacagcgcca
      781 ggccgtcgcc gtggcgaagt cggtgatgtg gaactcgaag ctcgtcatcc tcgacgagcc
      841 gacggccgcc ctcggcgtgg cgcagacccg ccaggtgctc gacctggtcc ggcgcctggc
      901 ggagaaggat ctcggcgtgg tgatcatctc gcacaacctg cacgacgtgt tcgaggtggc
      961 cgaccggatc acggtcctgc gcctcgggca acaggtgggg gtgtaccggc gcgaggccac
     1021 caaccagcag gaggtggtgc acgccatcac ggccggcacg cttgcccacg tgcccgggat
     1081 ggctgatgcc agggtggccg agcgatgacg accgagcccg acacctcgtc cggcgccgag
     1141 ccggtcgtcc ggggcccgga ggaggaggtc ctaccgccgg ccgcgccgcc ggtggaggtc
     1201 cccgccgaga cgctgggcgg ctacgcccgg cgctggtggg agaagatccg ggccggcgac
     1261 cttggctccc tgcccctcgt cgtcgggctg gtggtcatcg tcgccgtctt cggcggcctg
     1321 gactccacgt tcttcgtcga gcggaacttc accaacctcc tgctgcagat ggcgccgctc
     1381 gccaccatcg ccatcgggat cgtcttcatc ctgctgatcg gcgagatcga cctatccgtg
     1441 gcctacgtca gcgccgtcgg cggcgtgatg atgacgctcc tgctccgccc ggaggcctcg
     1501 aactggccgt ggtgggccgc catcggggtg gccgtcgcca tcaccaccgc catcggcttc
     1561 gtccacgccc tggtggtcac caaggccggc gtgccgtcct tcgtcgtcac gctcgccggc
     1621 ctgctgatct ggtctggcgt cgtcctgatc ctgaccacca cctactcgac cgccgggacg
     1681 atccggatcc agaacccgac ggtgatcggg ctggcgaaca acttcctgac gcacacgtgg
     1741 ggctggatcg tcgccattgg ggtggtggca ggctacgcgc tcacgcaatg gcagacggga
     1801 cgcgcgcggc gggccggcgg gctgtacgcg aagccggtct cgctcctcgc gctgcaggtc
     1861 gccggcctcg cggccgtcac gttcgccgcc gtctggtacg ccaaccgcga ccgcggcgtc
     1921 cccaccgtca cgctgatcct gctggcgctc ctggtgttct ggtcgttcat cgcggcacgg
     1981 acccggttcg ggcgccacgt ctacgcggtc ggcggcaacc ccgaggcggc ccgccgcgcc
     2041 gggatcggcg tggaccgcgt ccggatcatg gtgttcatga tcaacgggtt catggccggt
     2101 gtgggcggca tcctgttcgc ctcgcggctg cgctcggtgg ccacgggcac cggcggcggc
     2161 aacctgctgc tgagcgtcat cgccgccgcc gtgatcggcg ggacgagcct gttcggcggc
     2221 accgggcgcg tcgtcgcggc ccttcttggg gcgctgatga tcgcctcgat ccagaacggc
     2281 atgggactgc tgaacctggc gtcgggcccg cagttcgtga tcaccggtgg cgtcctgctc
     2341 gccgccgtgc tgatcgactc actgtcgaag cggcgacgcg ccgcccgcgg cgtgctctga
     2401 tcggccccgc cgaacggagg ggagcggatg agctacgaac cgcggcccga gcatcgcttc
     2461 tccttcggcc tgtggacggt cgggcacccc ggcttcgatc cgttcggcga tccggtccgc
     2521 gaggtcctct ccccggtgca gctcgtggag ctcctggcgg aggtcggcgc ctggggagtg
     2581 aacttccacg acaacgacct cgtcccgatc gacgcgagcg cgcacgagcg tgaccagatc
     2641 gtgtcggact tccgcaaggc gctcgacgcc accgggatcg tcgtccccat ggcgacgacc
     2701 aacctgttcc gggaccccgt gttccgcgac ggcgccttca ccgcccatga cccgcgcgtc
     2761 cgcgcgttcg cgttgcagaa gacgatgcgc gcgatcgacc tcggcgtcga gctcggggcg
     2821 acgacgtacg tgttctgggg cgggcgcgag ggcgtggaga ccgacgccgc gaaggacccg
     2881 ctcgtggcgt tcgagcgcta ccgggagggg atgaacttcc tgtgcgcgta cgttcgggac
     2941 cgcggatacg acctgcactt cgcgctcgag tcgaagccga acgagccgcg tggcgacatc
     3001 tacctgtcga cgaccggcgc gatgctcgcg ttcatcgaga cgctcgacca cgcggagatg
     3061 gtcggcgtca accccgaggt ggcgcacgag cacatggccg gcctgaactc gatgcatcac
     3121 gtggcccagg catgggcgat gggcaagctg ttccacgtcg acctgaacga ccagatcccg
     3181 ggccgctacg accaggacct gcggttcggc tccgggaacc cgaaggcggc gttcttcctg
     3241 gtcaagctgc tgggggacgt cgggtacgcg ggaagccggc acttcgacgc gcacgcatac
     3301 cgcacgtctg actacgagga cgtcaaggct ttcgcccggg gctgcatgcg cacctacctg
     3361 atcctcgagg agaaggccga gcgctggaac gccgaccccg atatccaggc gctggtggcc
     3421 gagatcaccg cggacgacgg ggcgatggcg gaggtcctcg gcccctacac gccggagcgg
     3481 gcggcggcgc tgtcgcgcct cgcgctcgac cggcgggcgc tcggcgcgcg cgggcagccg
     3541 tacgagcggc tcgatcagct gacgatggac ctgctgctcg gcgttcggtg acgctcgaga
     3601 ccgtccgtgt cgagggctac gagtgcgcac gcctcgacga cgggaacagc gcgctgacga
     3661 tcacggtgtc caccgggccg cgggtcctcg agctccgagc acggggcgag aacctgctgg
     3721 cgctggtgcc ggacgcgacc gtcccggtct ccgggggcgg gcgcctccgg ctgctcggcg
     3781 ggcatcgcct gtgggtggcg cccgaggtgc ccgcgatcac gtaccagccg gacgaccggc
     3841 cctgtcaggc gatcgaggtg gaggacggag ttcgcgtgac ggcgcccccg gacggggccg
     3901 ggctgacgaa gtcgctcgag gtgcggcggg cggcggacgg ctggaacgtc gatcacgaga
     3961 tccgcaacga ctcccacgag gcgatggagc tcgccccgtg ggctgtgacc atgctgcggc
     4021 cgggcggcga ggcggtgctc gccgccgcgg cggggggcga ggggctgcag gcggaccgcg
     4081 cgatcgtgct gtggccgtac acggatccgg ccgacgcgcg cgtctcgatc gagcgcgacg
     4141 cgcttcgggt ccgctgcgcg cccgggccca cgctcaagct cggtgtggcc tccctcgacg
     4201 gccgcgccac ctattcgctg aacggcgaga cgttcgagaa gcggaccgtc gtcgagcgcg
     4261 gcgcgcgcta cgcggaccgc ggggccgcgg tgcagctcta cgtcagcgac gcgttctgcg
     4321 aggtcgagac gctcgggccg ctggcgaccg tcgagcccgg cggcgccgtg gcgcaccgcg
     4381 agcgctggag gctctggtga gccacgtcct gggcctggac gtgtcgacga ccgcgacgaa
     4441 ggcggtcgtg gtggaccgcg gcggggaggt ccgggcggcc gcggcggccg actacccgta
     4501 cgagaccccg cgcccgctgt ggagcgagca ggaacccgcg cgctggtggg aggcgacggt
     4561 cgccgcggta cagcgcgccc tgggcgaggc cgggattcgc ggcgccgacg tcgaggcggt
     4621 cggcctggcc ggccagatgc acggcctggt cctgctggac gaggcgggcg cggtcctgcg
     4681 accggcgatc ctgtggaacg accagcgcac cggcgcggag tgcgacctga tccgggaggc
     4741 cgtcggcgcc gaggcgctca tcgccatcac ggggaatgac gcgctcccgg gattcaccgc
     4801 gccgaagctg ctgtgggtgc ggcggcacga gccggagatc tggtcccgca tcgcccacgt
     4861 gctgttgcc
//
LOCUS       DASPWO010000188         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_369157, whole genome shotgun sequence.
ACCESSION   DASPWO010000188 DASPWO010000000
VERSION     DASPWO010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369157"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VEO00_04220"
     CDS             complement(<1..438)
                     /locus_tag="VEO00_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HYU57238.1"
                     /translation="MPETLPRFASLAFDDIPRPDHADVAVVPLPPRATTDPAEWARVI
                     FDRGSIPVWVKALFVARQALVPVLGIPQGERGVFGVRKVVGEEALIAADDVHLDWRTG
                     VGVDAAAGLVRLTTAVRLHGRRGRLYFAPVRLLHPPIVRAMLTR"
     gene            493..1059
                     /locus_tag="VEO00_04225"
     CDS             493..1059
                     /locus_tag="VEO00_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYU57239.1"
                     /translation="MPRLGKDDWVTAGLAALREHGPDAVAVQPLAARLGATKGSFYWH
                     FATRDELLDAVLARWLEESTERVIADQERQHGDAAPAARFERLFEAVAAASEATPGEQ
                     RILAAAGHPAVGRAVRAAVERRIGYVTGLLMQAGLEDGPARRRALLAYMMYLGHAQLI
                     AVAPGELAAGQARQAREDAVRMLLGDTR"
     gene            complement(1170..>2002)
                     /locus_tag="VEO00_04230"
     CDS             complement(1170..>2002)
                     /locus_tag="VEO00_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HYU57240.1"
                     /translation="SGLSISQLVSTAWASAASFRGTDKRGGANGARIRLAPQKDWEVN
                     NPAELANVLQTLEQIRQGFNLSQSGGTKVSLADLIVLGGCAAVEQAAKNAGHDITVPF
                     APGRTDASQEQTDVETFAVLEPTADGFRNYLRAGEKLSPESLLLDRANLLTLTAPEMT
                     VLIGGMRALNANYGQSQHGVFTDRPETLTNDFFVNLLDMGTEWKASASAENLFEGRDR
                     ATGEVKCTATAVDLVFGSNSQLRAISEVYACDDAKEKFVRDFVATWDKVMNIDRFDVA
                     "
BASE COUNT          300 a          702 c          706 g          294 t
ORIGIN      
        1 gcgggtaagc atggcgcgga cgatcggtgg gtggaggagg cggacagggg cgaagtagag
       61 ccggccgcgc cggccgtgca ggcgcacggc ggtggtcagc cggaccagcc cggcggcggc
      121 gtccacgccg acgcccgtcc gccagtccag gtgcacatcg tccgcggcga tcagggcctc
      181 ctcgcccacc accttgcgga caccgaacac cccccgctcg ccctgcggga tgccgagcac
      241 cggcaccagc gcctgccgcg ccacgaacag cgccttcacc cacaccggga tcgacccccg
      301 gtcgaagatc acccgcgccc actcggcggg gtccgtcgtc gcccgcggcg gaagcgggac
      361 gacagcgaca tcggcgtggt ccggccgggg gatgtcgtcg aaggccagtg acgcgaaccg
      421 gggcagggtc tcgggcatcg tcggtctcct ccatacgccg gcgtacggtt tacgtacgct
      481 agcgtacgga acgtgccgcg gctagggaag gacgactggg tcacggccgg tctcgcggcg
      541 ttgcgcgagc acggccccga cgccgtcgcc gtccagccgc tcgccgcccg gctcggcgcc
      601 acaaagggct cgttctactg gcacttcgcc acccgggacg agctgctcga tgccgtcctg
      661 gcgcgctggc tggaggagag caccgaacgg gtgatcgccg accaggagcg ccagcacggc
      721 gacgccgccc ccgcggcgcg attcgagcgg ctgttcgagg ccgtcgcagc ggccagcgag
      781 gccacgccgg gcgagcagcg catcctcgcc gcggccggac acccggcggt cggccgcgcg
      841 gtccgggccg cggtcgagcg ccggatcggc tacgtcaccg gcctgctcat gcaggccggg
      901 ctggaggacg gcccggccag gcggcgggcc ctgctcgcct acatgatgta cctcggccac
      961 gcccagctga tcgccgtcgc ccccggcgag ctggccgccg gccaggcccg gcaggcccgc
     1021 gaggacgccg tccggatgct gctcggggac acccgctaga cgagtacgtc cttacggtgc
     1081 ggcggccgct gacgtggtcg gagggtgttc acgatcagtt tgtgacgaca gacctgaaca
     1141 cgtggccagg acggacctgg acatcgggat caggcgacgt cgaaccgatc gatgttcatg
     1201 accttgtccc acgtggccac gaagtcacgc acgaacttct ccttggcgtc gtcgcacgcg
     1261 tagacctccg agatggctcg gagctgggag ttcgaaccga agacgaggtc gacggcggtg
     1321 gcggtacact tgacctcacc cgtggcgcga tcccgaccct cgaacaggtt ctcagccgag
     1381 gcggatgcct tccactccgt gcccatgtcg agcaggttca cgaagaagtc gttggtcaac
     1441 gtctcgggcc ggtcggtgaa gacgccgtgt tgggactgcc cgtagttggc gttcagggcc
     1501 cgcatgccgc cgatcagaac cgtcatctcg ggagcggtca gcgtcaacag gttggcccgg
     1561 tccagcagca ggctctccgg cgacaacttc tctccggctc ggaggtagtt gcggaaccca
     1621 tctgcggtcg gttcgaggac tgcgaacgtc tccacgtcgg tttgctcctg cgaggcgtcc
     1681 gtgcgccccg gcgcgaacgg gactgtgatg tcgtgcccgg cgttcttcgc cgcttgctcg
     1741 acggccgcgc acccgcccag gacgatcagg tcagcgagcg agaccttcgt tccgccggac
     1801 tgtgagaggt tgaagccctg ccggatctgc tcgagggtct gcagcacgtt ggccagttcg
     1861 gccgggttgt tgacctccca gtccttttgc ggcgcaaggc gaatccgcgc cccgtttgcc
     1921 ccgccacgct tgtcggtgcc gcggaagctt gccgccgagg cccaagcggt ggagaccagc
     1981 tgggagatgg acagtcccga ct
//
LOCUS       DASPWO010000189         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_959722, whole genome shotgun sequence.
ACCESSION   DASPWO010000189 DASPWO010000000
VERSION     DASPWO010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959722"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VEO00_04235"
     CDS             complement(<1..1017)
                     /locus_tag="VEO00_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYU57241.1"
                     /translation="MRRTWQTILSTVAIAALLSSASFVAGFRSADPPPSGFDEIVQTA
                     QELRDRAARPVSDRELVDAAIQGMLDALGDPYANLLAPPEEETIDRLVDGSIVGIGVW
                     LDVEDRGLRITQIVPDTPAERAGLRAGDLILSVDGHTLAGLGQDGTDLLRGEAGSVAR
                     LQVERDDRERSVEVRRARIHVPNVSSRTLASGVGYVRLYQFGDGAAEDLRAALEDLLG
                     SGARGIVLDLRDNPGGLADEAYDAASLFIEDGVIATIRQRGRSEYDVRASGDALPDFP
                     LGVLVNGGSASASEILAGALKDRGRGLLVGERTYGKGSVLTVESLSEDTTIQFTTAFF
                     FSPSG"
     gene            complement(1113..2327)
                     /locus_tag="VEO00_04240"
     CDS             complement(1113..2327)
                     /locus_tag="VEO00_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYU57242.1"
                     /translation="MRAGSTTRRVLPSVLAAGLLLAPLAVPSAAHSPEDQLEQINAEL
                     DAKREWLAESGAREKSLVDQIAASDHRRAGVAARLEDLEAELAGAEARLAPVQAALDS
                     AVARHERLKVEVAETRDELDRQQAVLDAHAATSYKFGPGSYLDVILGASSFGELIDRT
                     ALATRVLMFDSDLVTELDATRARLAERRAMARESRETVEARRDDLRAERNRIRRLKEE
                     QEAAQAALDLEIAIRGAALEDIESSRAEYRASIAQLEADAAQITAILQGGGSIGDGDY
                     GGELYWPTAGSIGSGYGWRTHPVYGDQRFHAGVDIGGACGQPIWAAESGTVVSSTWSG
                     GYGLFTVVDHGDGLATAYAHQSQSYVSAGQRVTRGQTVGAVGTTGLSTGCHLHFEVRV
                     NGEPVDPVPYLT"
BASE COUNT          328 a          876 c          864 g          317 t
ORIGIN      
        1 tccagagggg ctgaagaaga acgccgtcgt gaactggatc gtcgtgtcct cggagagcga
       61 ctcgaccgtc aggaccgacc ccttcccgta ggtccgctcg ccgaccagca ggcctcgccc
      121 gcggtccttc agcgccccgg ccaggatctc cgacgcgctg gcgctcccgc cgttcacgag
      181 cacccccagc gggaagtccg gcagcgcgtc tccgctggcc cgcacgtcgt actcggaccg
      241 cccccgctga cggatggtgg cgatcacgcc gtcctcgatg aagaggctcg ccgcgtcgta
      301 ggcctcgtcg gccagcccgc cggggttgtc tcgcaggtcg agcacgatcc cccgggcgcc
      361 gctccccagc aggtcctcca gggcggcgcg gaggtcctcc gccgcgccgt cgccgaactg
      421 atagagccgc acgtatccca ccccggacgc gagggtccgc gacgatacgt tcgggacgtg
      481 aatccgagcg cggcgcacct cgacggagcg ctcccggtca tcccgctcga cctgcaggcg
      541 agccaccgag cccgcctcgc cacgcagcag gtcggtgccg tcctggccga ggcccgccag
      601 ggtgtgcccg tcgaccgaga ggatcaggtc cccggcccgg agcccggcgc gctcggccgg
      661 cgtgtccggg acgatctgtg tgatacgcag gccacggtcc tcgacgtcca accacacccc
      721 gatcccgacg atcgagccgt cgacgaggcg atcgatcgtc tcctcctcgg ggggcgccag
      781 caggttggcg taggggtccc cgagcgcgtc gagcatcccc tggatcgcgg cgtccaccag
      841 ctcgcggtcg ctgaccggcc gcgccgcgcg gtcccgcagc tcctgagccg tctggacgat
      901 ctcgtcgaac ccggagggcg gcgggtcggc cgagcggaac ccggccacga acgaggccga
      961 ggagagcagg gcggcgatcg ccaccgtaga gaggatcgtc tgccaggtgc ggcgcatgcg
     1021 aggtccgttt ccggtcgggg gtacgcccgc cgatgctacc ggatgccccg gcgagagaag
     1081 ccgggggccc acggacgcgg ccgccgcggc gatcaggtca ggtacgggac cgggtcgacc
     1141 ggctcgccgt tcaccctgac ctcgaagtgc aggtgacagc cggtggagag cccggtggtg
     1201 ccgaccgcgc cgaccgtctg cccacgggtc acgcgctggc cggcggagac gtagctctgc
     1261 gactggtggg cgtaggcggt cgccaggccg tcgccgtggt ctaccaccgt gaacaggccg
     1321 tagccacccg accaggtcga ggagaccacc gtccccgact cggcggccca aatgggctga
     1381 ccgcaggccc cgccgatgtc caccccggcg tggaaccgct ggtcgccgta gaccgggtgc
     1441 gtgcgccagc cgtagcccga accgatcgac ccggcggtcg gccagtagag ctcgccgccg
     1501 tagtcgccgt cgccgatcga gccgccgccc tgcaggatcg cggtgatctg cgcggcgtcg
     1561 gcctcgagtt gggcgatgga cgcccggtac tcggcgcggg aggactcgat gtcctcgagg
     1621 gccgctcccc ggatggcgat ctcgaggtcg agggccgcct gcgccgcctc ctgctcctcc
     1681 ttcaaccggc ggatgcggtt gcgctcggca cgcaggtcgt cgcggcgcgc ctcgacggtc
     1741 tcgcgggact cccgggccat ggcgcgacgc tcggccagcc gggcgcgcgt ggcgtcgagc
     1801 tccgtgacga ggtccgagtc gaacatcagc acccgggtgg ccagcgcggt ccggtcgatc
     1861 agctcgccga acgaggaggc gccgaggatg acgtcgaggt agctgccggg accgaacttg
     1921 tagctcgtgg cggcgtgggc gtccaggacg gcctgctggc ggtcgagctc gtcgcgcgtc
     1981 tcggcgacct ccaccttcag ccgctcgtgg cgagccacgg cgctgtcgag ggcggcctgc
     2041 accggcgcga ggcgggcctc ggcccccgca agctcggcct ccaggtcctc caggcgggcc
     2101 gcaaccccgg cgcggcggtg atcggacgcc gcgatctggt cgacgaggct cttctcgcgc
     2161 gccccggact ccgccagcca ttcgcgcttc gcgtcgagct cggcgttgat ctgctcgagc
     2221 tggtcctcag gagagtgggc ggcagaaggt acggcgagcg gggcaagcag cagccccgct
     2281 gccagcacgg agggaaggac ccgacgggtc gtcgagccgg cacgcatgga tcggtcagtc
     2341 tacggggccg attgccccgg ggcaacgacc tgtcgctaga cctcg
//
LOCUS       DASPWO010000190         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_28520, whole genome shotgun sequence.
ACCESSION   DASPWO010000190 DASPWO010000000
VERSION     DASPWO010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28520"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1877)
                     /locus_tag="VEO00_04245"
     CDS             complement(<1..1877)
                     /locus_tag="VEO00_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYU57243.1"
                     /translation="METRNEGIAIVGVGAILPDAPTAPAFWRNVKEGRYSITEVPPDR
                     WDPELYYDPDPKAPDKTYSKIGGWVKEWDWDPLGWHLPIPPRVSDAMDDGQKWALACT
                     REALLDYGYPERPLDPERTAVILGNALAGEKHYMTAIRVHFPAFAEELSNSPSFMALP
                     NDVQAAIVSEAHTGIGKRFPEITEDTMPGELSNVLAGRISNLFNFRGPNFTTDAACAS
                     AMAAMNAAVQGLRAGEYDSVITGGIDRNMGPHGFVKFCKIGALSATGTRPYAEGADGF
                     VMGEGAGLFLLKRLADAERDGDRIYAVIRSLAGSSDGKGKGITAPNSVGQRLAVERAW
                     GAIGISPATCTLVEGHGTSTAVGDVVEVESLQAAFGSPDVPAGSIALGSVKSNFGHLK
                     SAAGAAGMLKTVYALHEKVLPPSINFERPNPGIDWSGSPFKVNTELREWAQPASGPRR
                     AGVSAFGFGGTNFHAVLEEYVPGRTDGDGRKVVAVPEKPFASTGETGTGSPKPPLRGA
                     LVIGGVSPGAIAERLAAVKAEADAGRTPEPAAPAGEDLRAPERLAIDFADAADLATKG
                     EAALKALAADNPAVWKALKARGIFRGSGPAPKVAFLYTGQGSQYANMLRTLRDAEPIV
                     A"
     gene            complement(1913..>2807)
                     /locus_tag="VEO00_04250"
     CDS             complement(1913..>2807)
                     /locus_tag="VEO00_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU57244.1"
                     /translation="LGAGWAALDRAIPYSTERIQAGGPLSEKQGYTHKLIVPHAIRLE
                     AGRAYIEETAERIDAGEGSLNTEGAIAKYLATEAGLAAAEAAIQALGGYGYVKEYLVE
                     KIRRDVRITTIYEGTSEIMEMTIARDRWQEHLKSRGRHYHDVAEGLETLHAEFPTVGA
                     DVAALGMHALAEILERCRIARLTRNQHVLLRLGELIAYAECAGALARKAARADRSELS
                     PKADRRFEAEGLAAVSRVFAREAAFKVAHEGLRWVLGADPEVDAEALAASVRLTEIDR
                     AQGGLIDDMDVIADVLYGRAK"
BASE COUNT          360 a         1033 c          961 g          453 t
ORIGIN      
        1 tcggccacga tcggctcggc gtcgcgaagc gtccgcagca tgttcgcgta ctgcgaaccc
       61 tggcccgtgt acaggaacgc caccttgggc gcgggaccgg acccacggaa gatgccccga
      121 gccttcaggg ccttccacac cgccgggttg tccgcggcca gcgccttgag ggccgcctcg
      181 cccttggtcg cgaggtccgc ggcgtcggcg aagtcgatgg ccagtcgttc gggggcccga
      241 aggtcttccc ccgctggcgc cgccggctcg ggagtccggc ccgcatcggc ctcggccttc
      301 accgcggcaa gccgctcggc gatggcgccc ggggaaacgc ccccgatcac cagcgccccg
      361 cggagcggcg gcttgggcga gccggttccc gtttcgccgg tggaggcgaa gggcttctcg
      421 ggcacggcca cgaccttccg gccgtccccg tcggtccggc cgggcacgta ctcctcgagc
      481 accgcgtgga agttcgtgcc gccgaacccg aacgcactga ccccggcgcg gcgcgggccg
      541 gaggccggct gggcccactc ccgaagctcg gtgttgacct tgaacggcga gccgctccag
      601 tcgatccccg ggttcggccg ctcgaagttg atggagggcg gtagcacctt ctcgtgcagc
      661 gcgtagaccg tcttcagcat cccggccgcc ccggcggcgg acttcaggtg cccgaagttc
      721 gacttcaccg aacccagcgc gatcgagccg gcggggacgt cggggcttcc gaacgcggcc
      781 tgcaggctct ccacctcgac cacgtcgccc acggcggtgg acgtgccgtg tccctcgacc
      841 agcgtgcagg tggcgggcga gatgccgatc gctccccagg cccgctctac cgccagccgc
      901 tggcccaccg agttgggcgc ggtgatgccc ttccccttgc cgtcgctgga gcccgccagg
      961 gaccggatca cggcgtagat ccggtcgccg tcgcgttcgg cgtccgcaag gcgcttcagc
     1021 aggaacagcc cggccccctc gcccatcacg aagccgtcgg ccccctcggc gtacgggcgg
     1081 gtgccggtgg cggacagcgc cccgatcttg cagaacttca cgaacccgtg gggccccatg
     1141 ttccggtcga tcccgccagt gatcacggag tcgtactcgc cggcccgcag gccctggacc
     1201 gcggcgttca tggcggccat ggccgaggcg caggccgcgt cggtggtgaa gttcgggcct
     1261 cggaaattga acaggttcga gatccggccg gccagcacgt tggacagctc gccgggcatg
     1321 gtgtcctcgg tgatctcggg aaaccgcttg ccgatgcccg tgtgcgcctc agacacgatg
     1381 gcggcctgca cgtcgttcgg cagggccatg aagctgggcg agttcgacag ctcctcggcg
     1441 aaggccggga agtggacccg aatcgcggtc atgtagtgct tctcgccggc cagcgcgttg
     1501 cccaggatca ccgcagtacg ctcgggatcg agcggccgct ccgggtagcc gtagtcgagg
     1561 agcgcctcgc gcgtgcacgc cagcgcccac ttctggccgt cgtccatggc gtcgctgacc
     1621 cgcgggggga tcggaaggtg ccagccaagg ggatcccagt cccactcctt cacccagccg
     1681 ccgatcttcg agtacgtctt gtccggcgcc ttggggtccg ggtcgtagta cagctccggg
     1741 tcccaccggt cgggcgggac ctcggtgatg ctgtagcgac cctccttcac gttgcgccag
     1801 aacgccggcg ccgtgggcgc gtcgggcagg atggctccca ccccgacgat ggcgatcccc
     1861 tcgttcctgg tctccatgat gtcctcgctc ccgttccgtc gctttcctga ggtcacttgg
     1921 cgcgtccgta gagcacgtcc gcgatcacgt ccatgtcgtc gatcagtccc ccttgggccc
     1981 ggtcgatctc ggtgaggcga accgacgccg ccagggcctc agcgtccacc tccgggtccg
     2041 cgcccagcac ccaccgcagt ccctcgtggg ctaccttgaa cgcggcctcg cgggcgaaca
     2101 cgcgcgacac tgcggccagg ccctcggcct cgaaccggcg gtcagccttc gggctcagct
     2161 cgctccggtc ggccctagcc gccttccggg ccagcgcccc cgcgcattcg gcgtacgcga
     2221 tcagctcgcc caggcgcagc aggacgtgct ggttcctggt gagccgggcg atccggcacc
     2281 gctcgaggat ctccgccagc gcgtgcatcc cgagggcggc gacgtccgcg cccaccgtcg
     2341 ggaactcggc gtgcagcgtc tccagtccct ccgcgacgtc gtggtagtgg cgcccccggg
     2401 acttgaggtg ctcctgccac cggtcccggg cgatggtcat ctccatgatc tcggaggtgc
     2461 cctcgtagat cgtggtgatc ctgacgtcgc gcctgatctt ctcgaccagg tactccttca
     2521 cgtagccgta gccgccgagc gcctggatgg cggcttccgc ggcggccagg ccggcctcgg
     2581 tggccaggta cttggcgatg gctccctcgg tgttcaggga tccctcgccg gcgtcgatcc
     2641 gctcggcggt ctcctcgatg taggcccggc ccgcctccag ccgaatagcg tgcgggacga
     2701 tcagcttgtg ggtgtagccc tgcttctccg acagcggacc gccggcctgg atgcgctcgg
     2761 tggagtacgg gatggcgcgg tcgagcgcgg cccatccggc gccgagg
//
LOCUS       DASPWO010000191         3732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1049479, whole genome shotgun sequence.
ACCESSION   DASPWO010000191 DASPWO010000000
VERSION     DASPWO010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049479"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1255
                     /locus_tag="VEO00_04255"
     CDS             77..1255
                     /locus_tag="VEO00_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HYU57245.1"
                     /translation="MDEPREGQEPQVPLGDVLADIPLFREIQRVLLSGAGPVNWELAR
                     QVGIAVASWGKEDPLPSEEDRRAFEDMARAAELAVAEFTGLDAPAQVAAVTVHRRASW
                     VEANVSSLRPLVDPVASKLARLLSDVRGEGPPEAIGLGALVDRMAPLLLGAQVGTVLG
                     YLGQRVLGQYDVAVPTDAGTIAFVVPNIAEFEREWSLPPAELKEFVALHEVTHRFEFS
                     RPWVREHFQGMIRDFTERTEFDLAALEERLAGLDLSDPERLQEAFGNPSDLVDRVLDD
                     EGRLLLGRLQAFMSAAEGYGDHVMHAIGERMLPSHGQIDEALRRRREGASGETRLLER
                     LLGIEMKLDQYRLGRTFCDAVAERAGEEILARMWSDPEAMPSLPELEEPTLWLARTAA
                     "
     gene            1270..2052
                     /locus_tag="VEO00_04260"
     CDS             1270..2052
                     /locus_tag="VEO00_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57246.1"
                     /translation="MERGELIFANAVDDRVRTSGDDGGPTGAYVPSLPGRALPFWLIR
                     HYKAPAGLVGETIQFVAPSGRTTYEWGPRARRMVGMMDVTRQEDLITDARFVEGGVHL
                     ATFLLDEVPVGQFEFEVVLQASTGSLPAEVERGLKNTDVIWVGLEHEGRDVAAPSWYV
                     YQQGKLYVISDPDRKAGEQFVPGIPDSDEVVVITRREKGKDTALHRFMAAVRVIDPSS
                     PEYDRLAGVLADRRRSRVGPAQESIQRWKKAGVVIGELTPAV"
     gene            complement(2056..3078)
                     /gene="aroF"
                     /locus_tag="VEO00_04265"
     CDS             complement(2056..3078)
                     /gene="aroF"
                     /locus_tag="VEO00_04265"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HYU57247.1"
                     /translation="MVVVMKPTAADADVAHVVERVREAGGQAFVSPGKYRTIIGLVGD
                     TDRFMELPLASLPGVEQVVRVGRPYKLVARELHPEPTTVKIGSHPVDRHTITVIAGPC
                     AVESPEQAAEAARMAKGAGAHLLRGGAYKPRTSPYSFQGLAERGLEILAACRDATGLP
                     VVTEVMEARDVDLVAGTADALQIGARNMQNFALLKEVGRALLPVILKRGLSATVEEWL
                     MAAEYVAREGNTDIVLCERGIRTFETATRNTLDLAGMVVARQATHLPVIVDPSHATGQ
                     RSLVAPMARAAVAAGADGIMVEVHPHPEAALSDGPQALTGNDLEDLVSELAALGQALG
                     KAVPGR"
     gene            complement(3289..>3732)
                     /locus_tag="VEO00_04270"
     CDS             complement(3289..>3732)
                     /locus_tag="VEO00_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010142972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU57248.1"
                     /translation="HVGLFHTRWGLRVRAVGEHPRAADTVGIRVLFTRYRNVVLGGMV
                     AGIGGAYFTLGSVGRFNEGMTAGRGFIGLAAMIFGRWTPIGALGAGLVFGFADSLRVK
                     LSILNVPIPSEFLAMAPYLVTILVVAGVVGRARPPAADGKPYVKE"
BASE COUNT          520 a         1347 c         1352 g          513 t
ORIGIN      
        1 tgcgcatcgc ccgaccctac cgcggtggcg cgcgcggtcg ggtgcgaacc gagcgccctc
       61 cctacaatgg atgctcatgg acgagccgcg ggagggtcag gagccacagg tcccgctcgg
      121 cgacgtcctc gccgacatcc ccctgttccg ggagatccag cgtgtgctgc tttcgggcgc
      181 cggcccggtc aactgggagc tggcccgcca ggtcggcatc gcggtggcgt cgtggggcaa
      241 ggaggaccca ctcccgagcg aggaggatcg ccgcgcgttc gaggacatgg cccgggcggc
      301 ggagctggcg gtggccgagt tcaccgggct cgacgcgccg gcgcaggtgg cggcggtgac
      361 ggtgcaccgg cgagcctcct gggtcgaggc gaacgtctcc tcgctccgcc cgctggtcga
      421 ccccgtggcg tcgaagctgg cgcgcctgct gtcggacgtc cgcggggagg gaccgccgga
      481 ggcgatcggg ctcggggccc tcgtcgaccg gatggcgccg ctcctgctcg gggcgcaggt
      541 cggcaccgtc ctcggctacc tcgggcagcg ggtgctcggt cagtacgacg tggccgttcc
      601 caccgacgcc gggacgatcg ccttcgtcgt cccgaacatc gccgagttcg agcgcgagtg
      661 gagcctcccg ccggccgagc tgaaggagtt cgtcgcgctg cacgaggtga cccatcgctt
      721 cgagttctcc cggccgtggg tgcgcgagca cttccaggga atgatcaggg acttcaccga
      781 gcgcaccgag ttcgacctcg ccgcgctcga ggagcggctg gccgggctcg acctctccga
      841 ccccgaacgg ctgcaggagg cgttcggcaa cccgagcgac ctggtagacc gcgtgctcga
      901 cgacgagggc cgcctgctgc tcgggcggct gcaggccttc atgtcggcgg ccgagggcta
      961 cggcgaccac gtgatgcacg ccatcggcga gcggatgctc ccttcgcacg ggcagatcga
     1021 cgaggcgctg cgccgccggc gcgagggcgc gagcggcgaa acccgcctcc tggagcggct
     1081 cctcgggatc gagatgaagc tcgaccagta ccggctgggg cgcacgttct gcgacgcggt
     1141 cgccgagcgg gccggggagg agatcctggc ccggatgtgg tcggaccccg aggccatgcc
     1201 ctcgctgccg gagctggagg aaccgacgct ctggctcgcc cgcacggcgg cgtaactaga
     1261 atcacgcccg tggagcgcgg cgagttgatc ttcgcgaatg ccgtcgacga ccgcgtgcgg
     1321 acctccggcg acgacggcgg gccgaccggc gcgtatgtcc cgtcgctccc cgggcgcgcg
     1381 cttcccttct ggctgatccg ccattacaag gcgcccgccg ggctcgtcgg cgagacgatc
     1441 cagttcgtcg cgccctccgg ccggacgacc tacgagtggg ggccccgggc gcggcggatg
     1501 gtcgggatga tggacgtgac gcgccaggag gacctgatca ccgatgcccg cttcgtcgag
     1561 ggcggggtgc acctggccac gttcctgctc gatgaggtcc cggtcgggca gttcgagttc
     1621 gaagtcgtcc tgcaggcctc cacgggctcg ctgccggcgg aggtggagcg cggcctgaag
     1681 aacaccgacg tgatctgggt cgggctggag cacgaagggc gcgacgtggc cgccccgtcc
     1741 tggtacgtct accagcaggg gaagctctac gtgatctccg atcccgaccg caaggccggc
     1801 gagcagttcg tccccgggat cccggactcg gacgaggtcg tcgtcatcac ccggcgggag
     1861 aaggggaagg acaccgcgct gcaccggttc atggccgcgg tgcgcgtcat cgacccgtcg
     1921 agcccggagt acgaccggct ggccggcgtg ctggccgacc gccgccgtag ccgcgtcggc
     1981 ccggcgcagg agtcgatcca gcgctggaag aaggcaggcg tggtgatcgg cgagctcacc
     2041 cccgccgtct gacggtcagc gtcccggcac cgcctttccc agcgcctggc cgagcgcggc
     2101 caactccgag accaggtcct cgaggtcgtt cccggtcagg gcctggggac cgtcggacag
     2161 ggcggcctcc gggtgggggt gcacctcgac catgatcccg tcggcccccg ccgccaccgc
     2221 ggcgcgcgcc atcggcgcga ccagggaacg ctgccccgtg gcgtgcgacg ggtccacgat
     2281 caccggcagg tgggtggcct ggcgggccac gaccatcccg gccaggtcca gcgtgttgcg
     2341 cgtggccgtc tcgaaggtcc ggatgccgcg ctcgcagagc acgatgtcgg tgttgccctc
     2401 gcgggcgacg tactccgcgg ccatcagcca ctcctctacc gtcgccgaca ggccgcgctt
     2461 caggatcacc ggcaggaggg cgcgcccgac ctccttcagc agcgcgaagt tctgcatgtt
     2521 ccgcgccccg atctgcaagg cgtcggccgt cccggcgacc agatccacgt cgcgcgcctc
     2581 catgacctcg gtgacgaccg gcagcccggt ggcgtcccgg caggcggcca ggatctcgag
     2641 gcctcgctcg gcgagcccct ggaacgagta cggcgacgtg cgcggcttgt aggcgccccc
     2701 gcgcagcagg tgggccccgg cgcccttcgc catccgcgcc gcctcggcgg cctgctccgg
     2761 cgactcgacc gcgcacgggc cggcgatcac cgtgatcgtg tggcggtcca cggggtgcga
     2821 gccgatcttc accgtggtcg gctcgggatg cagctcgcgg gcgacgagct tgtacggccg
     2881 gccgacgcgc acgacctgct ccacgccggg gagcgaggcg agcggcagct ccatgaaccg
     2941 gtcggtgtcg ccgaccagcc cgatgatcgt gcggtacttg cccggggaga cgaacgcctg
     3001 cccaccggcc tcgcgcacgc gctcgaccac gtgcgccacg tcggcgtcgg ccgccgtcgg
     3061 cttcatcacg accaccatcg ctccacctct tctcgccggt gccggcccgg gaacgaagga
     3121 ccccgggccg ctggcccggg gtccgtgtcc ctttcggttt ctggtgacgt ggactacgcc
     3181 gggctgcgcc ccgcgaaata ccaccatccg ccaaagaacc gcttcatgtg aggggagatt
     3241 ctgcgtgatc tgcggacctc ctgcaacccc tcgcgggaac ccggaggttc attccttgac
     3301 gtagggcttg ccgtcggcgg cgggcggccg ggcacggccg accacgccgg ccaccaccag
     3361 gatcgtcacc aggtagggcg ccatcgccag gaactccgag gggatcggca cgttcaggat
     3421 cgacagcttg acccgcagcg agtcggcaaa gccgaagacc agcccggcgc cgagcgcgcc
     3481 gatcggggtc cagcgcccga agatcatcgc ggcgagcccg atgaacccgc gcccggccgt
     3541 catgccctcg ttgaagcgcc cgaccgaccc gagcgtgaag tacgccccgc cgatcccggc
     3601 gaccatcccg ccgaggacga cattgcggta gcgcgtgaag agcacgcgga tgccgacggt
     3661 gtccgccgcg cgcgggtgct cgccgacggc ccgcacgcgc aggccccagc gggtgtggaa
     3721 cagcccgacg tg
//
LOCUS       DASPWO010000192         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_960394, whole genome shotgun sequence.
ACCESSION   DASPWO010000192 DASPWO010000000
VERSION     DASPWO010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960394"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VEO00_04275"
     CDS             complement(<1..528)
                     /locus_tag="VEO00_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57249.1"
                     /translation="MAIKGKSKGRSAKAVTRGPKPVYQPVKKRLIAKREFWLVILSIL
                     GVMVVVGLVAGFVAERNSSEQDELEQRMRATMSSFQGQVEPVLATIGQAQPPAGYQVF
                     TDLSTAVDNLVAEKPDAPVDPKALTQTAGDAATSAKTALEALQSIDVMALIRGKGFSE
                     DFVLHVINAKDNFVQA"
     gene            complement(540..1325)
                     /gene="tatC"
                     /locus_tag="VEO00_04280"
     CDS             complement(540..1325)
                     /gene="tatC"
                     /locus_tag="VEO00_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HYU57250.1"
                     /translation="MMKLIPERLRRRSEPDPDGTMTLVDHLSELRYRLLVVIAAIGVG
                     AIFGWFLFDRVIDLLLNPYCDYWRTVPRGVRATRDCALFFSGALEPLVIKLKLVGFLG
                     LFLALPVVLYQVWAFIVPGLTRRERRMAIPFVASSVLLFALGALVAYWTLPKALNFLL
                     GFAGSQFAPLLTADRFLTFVMLVALSFGLAFEFPIVLVFLLLAGAVTTRQLRDWRRGA
                     ILFIAVFAAIITPSSDPYTMLAMTIPMVLFYEAAIIVGRLLKR"
     gene            complement(1336..1944)
                     /locus_tag="VEO00_04285"
     CDS             complement(1336..1944)
                     /locus_tag="VEO00_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HYU57251.1"
                     /translation="MLNIGPQELLIILVIALIVVGPQRLPALGRSIGRGLRELRKAQD
                     EVKRTIQVNLDDEPPNGRSGASPTGRVRPGGRADAHAADPSAEPATTTGEPGPSVADA
                     TPTASEPLPPDEVREISRSLGRSLAELRRAKEEVQRSFRIDLTPATRSPEPKRARPAP
                     PEPSTAAADTGGPSATPASSPAALPAEPTPDGPAATPDRASD"
     gene            complement(2042..2263)
                     /locus_tag="VEO00_04290"
     CDS             complement(2042..2263)
                     /locus_tag="VEO00_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57252.1"
                     /translation="MTTTALVWLVVGLLSLVAVLAVLIALTRHLFVVGRAAGRFQDEV
                     GPLTQEIGELADAASDRSRSMPRGPRSGP"
BASE COUNT          369 a          792 c          835 g          330 t
ORIGIN      
        1 cgcctggacg aagttgtcct tcgcgttgat cacatgaagg acgaagtcct cggagaaccc
       61 cttgccacgg atcagcgcca tcacgtcgat cgactgcagc gcctcgaggg cggtcttggc
      121 agaggtcgcg gcgtcgcccg ccgtctgcgt gagcgccttc ggatcgaccg gcgcgtccgg
      181 cttctccgcc acgaggttgt cgacggccgt cgacaggtcc gtgaacacct ggtagccggc
      241 cggaggctgc gcctggccga tcgtcgcgag cacgggttcg acctgtccct ggaaggagct
      301 catggtggcc cgcatccgct gctcgagctc gtcctgttcg ctcgagttgc gctcggcgac
      361 gaacccggcg accagcccga cgaccaccat gacacccagg atgctgagga tcaccagcca
      421 gaactcgcgc ttggcgatca ggcgcttctt gaccggctga tagacgggct tgggcccgcg
      481 ggtgacggcc ttcgcgctcc gccccttgct cttgcctttg atcgccatgc gagatccacc
      541 tagcgtttca ggagacggcc cacgatgata gcggcttcgt agaacagcac catcgggatc
      601 gtcatggcaa gcatcgtgta cgggtccgag ctcggcgtga tgatcgccgc gaagaccgcg
      661 atgaacagga tcgccccccg ccgccagtcc cgcagctgcc gggtcgtcac ggcgccggcg
      721 agcagcagga acacgagcac gatcgggaac tcgaacgcga gcccgaacga gagcgccacg
      781 agcatcacga acgtcaggaa ccggtcggcg gtcagaagcg gcgcgaactg gctccccgcg
      841 aagccgagca ggaagttgag ggccttcggg agcgtccagt aggcgaccag cgccccgagc
      901 gcgaacagga ggaccgagga ggccacgaac gggatcgcca tccttcgctc gcgacgggtg
      961 aggccgggca cgatgaaggc ccagacctgg tacagcacca ccggcaacgc aaggaacagc
     1021 ccgaggaacc cgaccagctt cagcttgatc accagcggtt cgagcgcccc ggagaagaac
     1081 agggcgcagt ctcgcgtggc tcggacgccg cggggcacgg tccgccagta gtcgcagtag
     1141 gggttcagca acaggtcgat cacgcggtcg aacaggaacc acccgaagat cgccccgacc
     1201 ccgatcgcgg cgatcacgac gagcaggcgg taccgcagct ccgacagatg gtcgacgagc
     1261 gtcatcgtgc cgtccgggtc cggctcggag cgccgccgga ggcgttccgg gatcagcttc
     1321 atcatcgacc cggggtcagt cgctcgcgcg atcgggcgtg gccgccggtc cgtccggcgt
     1381 gggttccgcg ggcaacgcgg cggggctcga ggccggcgtc gccgacggtc cgccggtgtc
     1441 ggccgccgcc gtcgatggtt ccgggggtgc gggccgcgct cgcttcggct ccggggatct
     1501 cgtcgcgggc gtgagatcga tccggaagga gcgctggacc tcctccttgg cacgccggag
     1561 ctccgccagg ctccgcccca gcgaacggga gatctcccgg acctcgtcgg gcggcagcgg
     1621 ttcggaggcg gtgggggtgg cgtccgccac cgacgggccc ggctcgccgg tcgtcgtcgc
     1681 cggttcggcg ctcgggtccg cggcgtgagc atcggcgcgg cctccgggcc tcacgcgacc
     1741 ggtgggactc gcacccgagc ggccgttcgg aggctcgtcg tccaggttga cctggatcgt
     1801 ccgcttgacc tcgtcctgcg ccttgcggag ctcgcgcaga ccgcgcccga tcgaccgtcc
     1861 gagcgcgggc aggcgctgtg gcccgaccac gatcagggcg atcaccagga tgatcaagag
     1921 ctcctgcgga ccgatgttca gcatctcgtc ctgcgctcca ggcggcgccc cagacggcgc
     1981 tccaggtcgg cgatccgggg gcgagagttc ctcgtgaggg ccatcgtact gccgcctcgg
     2041 ttcaggggcc ggacctcggt ccgcgaggca tgcttcggct ccggtcggag gcggcgtccg
     2101 cgagctcgcc gatctcctgc gtgaggggcc cgacctcgtc ctggaagcgg ccggccgcgc
     2161 ggccgaccac gaacaggtgc cgggtcagcg cgatcagcac ggcgaggacg gcgacgagcg
     2221 acagaagccc gacgacgagc cagacgagcg cggtcgtggt catcgggtgt cgatggtagc
     2281 ggaggggacg gccgagcgag gttcagtgcg cggtgaggaa ctgctc
//
LOCUS       DASPWO010000193         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_796949, whole genome shotgun sequence.
ACCESSION   DASPWO010000193 DASPWO010000000
VERSION     DASPWO010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796949"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..798)
                     /locus_tag="VEO00_04295"
     CDS             complement(76..798)
                     /locus_tag="VEO00_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57253.1"
                     /translation="MMLEFSNDLRQALGVWRRSPALPTTSLALGLVYLVPEPYALGMF
                     PLILFGIGWVGTERIWYLRSFRGRSISFGEIWRFTWGFLGRFVALGVLVLLVYVPLFL
                     AFAPTGNLSLLLAIATILTIPVDVALTFVTPALTYSTRRATTAIGLGLQTLRDEWPRC
                     AVYALVPPLAFIVASLLSPTQAVVQALLSGAAMLFNLWVKGATAAFYLRRHDTGDDGA
                     AFIPRGQETWDQPREDWRNPLG"
     gene            complement(795..1214)
                     /locus_tag="VEO00_04300"
     CDS             complement(795..1214)
                     /locus_tag="VEO00_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HYU57254.1"
                     /translation="MSEPRPGLEAEASLDVTDAVTAARVGSGGVPVLATPEVVALVER
                     AVVAALERALAEGQTTVGARIELDHLAPTPVGGTATARARLDDVDGRRLTFSFTVTDD
                     AGEIARGTHVRVVVDLKRFLAAAGERVPRPQPDRSAG"
     gene            complement(1253..1615)
                     /locus_tag="VEO00_04305"
     CDS             complement(1253..1615)
                     /locus_tag="VEO00_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57255.1"
                     /translation="MHPIIVGLAALAGASLLSVVVGIGTGMVAAEVQGPVEGLAVLAP
                     LIVGLGTRSTPGRVRCSAPGWPRAARRGRVARSCSSPLLHPVAVGLAYVLARGIGRED
                     AVYGIGAAAVVLAERPSG"
     gene            1654..>2154
                     /locus_tag="VEO00_04310"
     CDS             1654..>2154
                     /locus_tag="VEO00_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57256.1"
                     /translation="MEGPAGVLALVLAETAVGGSLFLWLAPLWGRVREGFFTLTAAIL
                     AALALAAGGAAASGRVAGASPAPQLLMLALGVLLALSAIANLARQRTIARVLGLLSAG
                     VGVAGLGMLVPLSDASTALAAFQLAAGAAFMGAVMDGLLLGHWYLVDRRLSRDPINLF
                     AVALLVS"
BASE COUNT          312 a          738 c          817 g          287 t
ORIGIN      
        1 gttccgaagg ccggacggct cgatgctgga ggagcgtggg ccgccctcca tcctgaccta
       61 actgaggaag gccagtcagc caagtggatt gcgccagtcc tcgcgtggtt ggtcccaggt
      121 ctcctggccg cgtggaatga acgccgcccc gtcatcgccg gtgtcgtggc ggcggagata
      181 gaaggcggcg gtggctccct tcacccatag attgaatagc atcgccgccc cactgagcag
      241 agcctgcacg actgcctggg tcggcgagag gagtgaagcg acgatgaacg ccagtggcgg
      301 taccagcgcg tacacggcgc accgcggcca ctcgtctcgg agcgtttgca gcccgaggcc
      361 gatcgctgtc gtcgcccggc gcgtggagta ggtgagggct ggcgtgacga acgtcagggc
      421 cacgtcgacc gggatcgtca ggatcgtggc gatggccagc agcaggctca ggttgcctgt
      481 cggcgcgaag gcgaggaaga gcggaacata aactaggagc acgaggaccc cgagcgcgac
      541 gaaacgcccc aagaagcccc aggtgaagcg ccagatctcc ccgaaggaga tcgagcggcc
      601 tcggaaggac cgcaggtacc agatgcgttc ggtccccacc catccgatcc cgaacaggat
      661 gagagggaac atgccgagtg catagggctc cggtacgagg tacacgaggc cgagcgccaa
      721 cgacgttgtg gggagcgccg ggctgcgcct ccagacaccg agtgcctgtc gaaggtcgtt
      781 gctgaactcc agcatcaccc ggcgctccta tccggctgcg gtcgggggac ccgttctccg
      841 gcggcggcca ggaaccgttt gagatcgacc acgacgcgga cgtgggtgcc gcgggcgatc
      901 tcgccggcgt cgtcggtgac ggtgaaggag aatgtcaggc ggcggccgtc gacgtcgtcc
      961 aggcgggcgc gggcggtggc ggtcccgccg accggcgtgg gggcgagatg gtcgagctcg
     1021 atgcgggccc ccacggtcgt ctggccctcg gcgagagccc gctcgagggc cgcgacgacg
     1081 gcccgctcga cgagcgccac cacctccggc gtggcgagga cgggcacgcc accggaaccg
     1141 acccgcgcgg cggtcacggc gtcggtcacg tcgagggagg cctcggcttc gagacccgga
     1201 cgcggctcgc tcacgaggtg aggctacccc cggggcgtcc cggcgatggc gatcacccgg
     1261 acggccgctc agcgaggacc accgcggcgg cgccgatgcc gtagacggcg tcctcgcgcc
     1321 cgatcccgcg cgccaggacg tacgcgaggc cgacggcgac ggggtgaagg aggggcgacg
     1381 agcaggaccg tgcgacgcga ccgcgccgtg cggcgcgcgg ccagccaggc gccgagcacc
     1441 gcacccggcc aggtgtcgaa cgcgtcccca ggccgacgat cagcggcgcc agaaccgcca
     1501 ggccctcgac cgggccctgc acctcggcgg cgaccatccc cgtgccgatc ccgaccacga
     1561 cggagaggag cgacgccccg gcgagggcgg ccagcccgac gatgatcggg tgcacgtgtt
     1621 cgcgacccgt gacgggtcgc taccctaccg gccgtggagg gtccggcggg ggtgctggcg
     1681 ctggtgctgg cggagacggc ggtcgggggc tcgctgttcc tgtggctggc cccgctctgg
     1741 gggcgcgtgc gggaaggctt cttcacgctg accgcggcga tcctggcggc cctcgcgctg
     1801 gccgcgggcg gcgccgccgc gtccgggagg gtcgccggcg cctcgcccgc gccgcagctg
     1861 ctcatgctgg ccctcggtgt gctcctggcg ctgtccgcga tcgcgaacct ggcgcggcaa
     1921 cgaacgattg cccgggtcct cggcctgctc tcggcgggcg tgggggtggc cggtctgggc
     1981 atgttggtgc cgctgtccga cgcctccacg gcgctcgccg cgttccagct cgcggcgggg
     2041 gcggcgttca tgggtgccgt catggacggg ctgctgctcg ggcactggta cctggtggac
     2101 cgccggctct cccgcgaccc gatcaacctg ttcgcggttg cgctcctcgt ctcg
//
LOCUS       DASPWO010000194         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_370461, whole genome shotgun sequence.
ACCESSION   DASPWO010000194 DASPWO010000000
VERSION     DASPWO010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370461"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VEO00_04315"
     CDS             <1..276
                     /locus_tag="VEO00_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HYU57257.1"
                     /translation="GFITVAPIEPAAALVYARTHGHEWILPQADALVAAGPGIVEEAH
                     GSALRVGTWTVDDEDRLAELFAWGVDAVASNDPALAVRVRDRVRAAR"
     gene            complement(290..1207)
                     /locus_tag="VEO00_04320"
     CDS             complement(290..1207)
                     /locus_tag="VEO00_04320"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYU57258.1"
                     /translation="MGKRLLLIANANAKTMTPRVREVITKALSADYQVRVADTKRHGH
                     AIHVARGAAHEGFDLVVALGGDGTVNEVANGLAGSDVPLAVIPGGGTNVFARSLGIPQ
                     DPVEAVAELLENGDRGPRRIPLGRVDDRYFVFGCGMGFDAAIVRQVERRQRLKKAVGE
                     WYYVWSGLRIFLTRYDRRRPHVRMSWGEHLEHERTGLFLGIAQKASPFTYLGDRELRV
                     CPDVTPEGGIDCFALDSLKVFRTVRIAVQAFGSAKHVRNRHAVYVHDEARIRLESDVP
                     LPVQMDGEYLGERTVVDMHCVPDALALLG"
     gene            complement(1219..1983)
                     /locus_tag="VEO00_04325"
     CDS             complement(1219..1983)
                     /locus_tag="VEO00_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HYU57259.1"
                     /translation="MTEAGERRAVDRAQLKEQFRRYRETGDQDLRTALIEAHLGLVEY
                     LARRFAGRGEQLDDLVQVGTIGLLKAVDRFEPHREVEFSTYATPTIVGELKRHFRDRG
                     WAVRVPRRLQELSLQVSKVVARLSQDRGRSPTVAEIALEAGATEDEVLEALDSAHAYS
                     LISLDAPATDEGGPLHETLGSEDEMLDVLEHRASLAPMLKRLPARERHLLYLRFFKGL
                     TQSEIARELSISQMHVSRLLTRTLAQLRDELTDEGS"
     gene            complement(1980..2354)
                     /locus_tag="VEO00_04330"
     CDS             complement(1980..2354)
                     /locus_tag="VEO00_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57260.1"
                     /translation="MSGRLVIRIPPATAYAGLVRAAASALAARLDFTYDQIMDLHIAV
                     DEICSRLLATTAAPTALEVVLEARDGALVVHARAEGSARPDRPFLNEWSERILRSVVD
                     RFTVEQRDGSTTVRLDVARAGR"
     gene            2493..>3001
                     /locus_tag="VEO00_04335"
     CDS             2493..>3001
                     /locus_tag="VEO00_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYU57261.1"
                     /translation="MGAINLVTELPGPRSRAVFDRKRRVVADPLDLHAPVVISEGRGA
                     VITDVDGNTLLDFSGGLGCQITGYSHPRVVEAVTRQAARFSHTDFSVIAYEVYVELAE
                     RLVALSGGDRRAAFFNSGAEAVENAVKFAKAATGRPGVVAFEGAFHGRTLLTMTLTSR
                     YHPYKAGFGP"
BASE COUNT          402 a         1090 c         1087 g          422 t
ORIGIN      
        1 gggttcatca ccgtcgcccc gatcgagccg gccgcggcgc tcgtctacgc gcggacgcac
       61 gggcacgagt ggatcctccc gcaggccgac gccctggtgg cggccggccc ggggatcgtg
      121 gaggaggcgc acggctctgc cctgcgcgtc ggcacctgga ccgtggacga cgaggatcgg
      181 ctcgccgagc tgttcgcgtg gggcgtggac gccgtggcct ccaacgaccc cgccctcgcc
      241 gtgcgcgtgc gcgaccgggt ccgcgccgcc cgctagatcg ctcgccgagc tagccgagca
      301 acgccagggc gtcgggaacg cagtgcatgt ccaccaccgt gcgctcgccg aggtactcgc
      361 cgtccatctg caccggcagc gggacgtcgc tctccaggcg gatgcgcgcc tcgtcgtgga
      421 cgtagacggc gtgccggttg cgcacgtgct tggccgatcc gaacgcctgc actgcgatcc
      481 gcaccgtgcg gaacaccttc agcgagtcga gcgcgaagca gtcgatcccg ccctcgggag
      541 tcacgtccgg gcagacccgg agctcccggt cgccgaggta ggtgaacggc gacgccttct
      601 gggcgatccc caggaacagg ccggtccgct cgtgctccag gtgctcgccc caggacatcc
      661 ggacgtgcgg acggcggcgg tcgtaccgcg tcaggaagat ccgcaggccg gaccacacgt
      721 agtaccactc gccgaccgcc ttcttcagtc gctgccgccg ctccacctgg cggacgatcg
      781 cggcgtcgaa ccccatcccg cagccgaaca cgaaatagcg gtcgtcgacc cggccgagcg
      841 ggatccgccg cgggccacgg tcgccgttct ccaggagctc ggcgacggcc tcgaccgggt
      901 cctgcgggat gccgagcgag cgggcgaaga cgttcgtccc gccgccgggg atcaccgcca
      961 ggggaacgtc ggatccggcc agcccgttcg ccacctcgtt gacggtcccg tcgccgccga
     1021 gggccaccac cagatcgaag ccctcgtggg cggcgccgcg cgcgacgtgg atcgcgtggc
     1081 cgtggcgctt cgtgtcggcc acccggacct ggtagtcggc cgacagggcc ttggtgatga
     1141 cctcacgcac gcgcggggtc atggtcttcg cgttggcgtt cgcgatcagc agcagtcgct
     1201 tccccacggg cacccgcctc aggacccctc atcggtcagc tcgtcgcgga gctgggcgag
     1261 cgtgcgggtc aggaggcgcg agacgtgcat ctgcgagatc gacagctcgc gcgcgatctc
     1321 gctctgcgtc aggcccttga agaagcgcag gtacagcagg tggcgctcgc gggcggggag
     1381 ccgcttgagc atcggggcga gactggcgcg atgctcgagc acgtcgagca tctcgtcctc
     1441 gctgcccagg gtctcgtgca gcgggccacc ctcgtcggtg gccggcgcgt cgagcgagat
     1501 cagcgagtag gcgtgcgccg agtcgagcgc ctcgaggacc tcgtcctcgg tggcgcccgc
     1561 ctccagcgcg atctcggcga ccgtcggcga tcgcccccgg tcctgggaca ggcgggcgac
     1621 gaccttcgac acctgcaggg agagttcctg gagccgccgc gggacgcgca cggcccagcc
     1681 ccggtcgcgg aagtggcgct tcagctcgcc gacgatcgtc ggcgtggcgt aggtcgagaa
     1741 ctccacctcc cggtgcggct cgaaccggtc caccgccttc agcaggccga tcgttccgac
     1801 ctgcaccagg tcgtcgagct gctcgccgcg cccggcgaag cggcgggcca ggtactcgac
     1861 cagcccgagg tgcgcctcga tcagcgcggt gcgcagatcc tggtcgccgg tctctcggta
     1921 ccggcggaac tgctccttca gctgtgcccg gtccacggcg cggcgctccc ccgcctcggt
     1981 catcggccgg cccgggccac gtcgaggcgg acggtggtgg acccgtcacg ctgctccacc
     2041 gtgaagcgat cgaccaccga ccgcaggatc cgctcggacc actcgttcag gaacggccgg
     2101 tcggggcggg ccgatccctc ggcccgcgcg tgcacgacga gggcgccgtc gcgggcctcg
     2161 agcacgacct ccagggcggt gggagccgcg gtcgtcgcca gcaggcgcga gcagatctcg
     2221 tccacggcga tgtgcaggtc catgatctgg tcgtaggtga agtcgagccg ggcggcgagg
     2281 gcgctggcgg ccgcgcggac caggcccgcg tacgcggtgg ccggcgggat gcgaatcacg
     2341 agtcgtccac tcatcggctc ggccatccta cgatgagctc gtgccgcctc cccccgtcgg
     2401 ccgtgccgcc ggtgcacggt ccaccgccgg ggggcgtccg gcgtgggccc ggcgctctgg
     2461 tagcctcgcg cacgaggaag cgaggaggag cgttgggcgc catcaacctc gtcacggagc
     2521 tgccggggcc tcgttcgagg gccgtcttcg accgcaagcg gcgggtggtc gccgacccgc
     2581 tcgacctgca cgcgccggtg gtgatctccg aggggcgcgg ggccgtcatc accgacgtgg
     2641 acggcaacac gctcctcgac ttcagcggag ggctcggctg ccagatcacc ggctactccc
     2701 acccgcgggt ggtcgaggcg gtgacccggc aggcggcgcg cttctcgcac accgacttct
     2761 cggtgatcgc ctacgaggtc tacgtcgagc tggccgagcg gctggtggcg ctctccggcg
     2821 gcgaccgtcg ggcggcgttc ttcaactcgg gtgcggaggc cgtcgagaac gcggtgaagt
     2881 tcgcgaaggc ggccaccggc cgccccggcg tggtcgcgtt cgagggggcg ttccacgggc
     2941 gcacgctcct gacgatgacg ctgaccagcc gctaccaccc ctacaaggcc gggttcggtc
     3001 c
//
LOCUS       DASPWO010000195         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_797034, whole genome shotgun sequence.
ACCESSION   DASPWO010000195 DASPWO010000000
VERSION     DASPWO010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797034"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VEO00_04340"
     CDS             <1..361
                     /locus_tag="VEO00_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57262.1"
                     /translation="TLGPDGTFGVAVNPTATTDYRLITGAFKAFPLRVNVAPVVTLTR
                     APDNTALFGTTNPVLTGGTVEVQRQDGIVWTTAATASVNAGAFSVPLPLAPGTYRARF
                     APGGGLVAGTSAPLLLG"
     gene            358..1803
                     /locus_tag="VEO00_04345"
     CDS             358..1803
                     /locus_tag="VEO00_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYU57263.1"
                     /translation="MKRLVLAALGAAALAAATSAAAFAPLDPLSSRQWYLTQDRAFDY
                     WTAPEDATLVPVKVAVIDSGIDGRHPDLAAKVVAARSFVGGSPYRDRQGHGTFVAGVI
                     AASLDNGEGIAGIAFNAQLVVAKVVKQNGSVPLGAEVAAIRWAADQGARVINLSLGGV
                     RDPLDPDKDTYSPLEAAAVQYAFAKGAVVVAAVGNGPQSPSTPWPYAHYPAALPHVLG
                     VGALARNGEVPAFSNRDSVYVDIAAPGTGIVSTFPLALTAETPSCTEQGYSICAPGSF
                     RAEGTSFAAPQVTAAAALVLGIRPDFAPEQVVGVLERTAEDVRTLTGCRRCPLGRDRF
                     SGWGRLNILDALTEAASTEPPTDRFETNDNAGAQAFRLYGRDRTLTATIDYWDDQIDV
                     YAVRIRRGERLFARLSSSPGAAAKLLLWKPETETVEGLLVPTSARAAQSSHVGVQERL
                     AYTARDEGWYYLEVVLERPGAGPYTLSYAKS"
     gene            complement(2016..>2249)
                     /locus_tag="VEO00_04350"
     CDS             complement(2016..>2249)
                     /locus_tag="VEO00_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HYU57264.1"
                     /translation="FMGRTERRHALMKGPTIRRGARVGGGAILCPAVEIGEEAFVGAG
                     AVVTKDVPPRMVVVGSPARVLRAVADDELLENQ"
BASE COUNT          309 a          873 c          750 g          317 t
ORIGIN      
        1 gacgttgggg ccggacggaa cgttcggcgt cgccgtcaac ccgaccgcca cgaccgatta
       61 ccgcctcatc accggtgcgt tcaaggcgtt tccgctgcgc gtcaacgttg ccccggtggt
      121 gaccctcacg cgcgccccgg acaacacggc gctcttcgga acgaccaacc ccgtcctcac
      181 cggcggcacc gtcgaagtcc agcgccagga cggcatcgtc tggacgaccg ccgccacggc
      241 cagcgtcaac gccggcgcct tcagcgtccc gctgccactt gcacccggca cctaccgagc
      301 ccgcttcgcg cccggcggcg gcctcgtcgc gggcacctcg gcgccgctcc tgctcgggtg
      361 aagcggctcg ttctcgccgc tctcggggca gccgccctcg ccgcggcgac ctccgccgcg
      421 gcgttcgctc cgctcgaccc gctgtcctcc cggcagtggt atctgaccca ggaccgtgcg
      481 ttcgattact ggacggcgcc cgaggacgcg acgctcgtgc cggtgaaggt ggctgtgatc
      541 gactccggga tcgacggccg ccatccggat ctcgctgcga aggtcgtcgc cgcgcggagc
      601 ttcgtcggcg gctcgccgta ccgggaccgg cagggccacg gaacgttcgt ggccggcgtg
      661 atcgcggcaa gcctcgacaa cggtgagggg atcgcgggga tcgccttcaa cgcccagctg
      721 gtcgtcgcga aggtcgtcaa gcagaacggc tcggttccgc tcggcgccga ggtggccgcg
      781 attcgctggg cggccgacca gggcgcgcgc gtgatcaacc tcagcctcgg cggagtccgc
      841 gacccgctcg atcccgacaa ggacacctac tcgccgctcg aggcggcggc cgtccagtac
      901 gcgttcgcca agggcgctgt cgtcgtcgcc gcggtcggga acgggcccca gtcgccgtcg
      961 acgccgtggc cttacgcgca ctacccggcg gcgctgccgc acgttctggg cgtcggtgcg
     1021 ctggcacgga acggcgaggt gccggcgttc tcgaaccggg actccgtcta cgtcgacatc
     1081 gcggccccgg ggaccgggat cgtctcgacc ttcccgctcg ctctcaccgc cgagacgccg
     1141 tcctgtaccg agcagggcta ctcgatctgc gccccgggga gctttcgcgc cgagggcaca
     1201 tccttcgcgg cgccacaggt cacggccgcc gccgccctcg tgctcggcat tcgcccggac
     1261 ttcgcgcccg agcaggtcgt cggcgtgctc gagcggactg ccgaagacgt gaggaccttg
     1321 acgggctgcc gccgctgccc actgggccgc gatcggttca gcggctgggg acgcctgaac
     1381 atcctcgacg cgctgacgga ggcggcctcc accgagccgc ccacggaccg gttcgagacg
     1441 aacgacaacg ccggcgcgca ggccttccgc ctctatggcc gcgaccgcac gctgacggcg
     1501 acgatcgact actgggacga ccagatcgac gtctacgcgg tgcgaatcag gcgcggtgag
     1561 cgcctgttcg cccggctgag ctcctcgccc ggggccgcgg cgaagctcct gctgtggaag
     1621 cccgagacgg agaccgtcga aggcctgctg gtgccgacct cggcccgcgc cgcccagtcg
     1681 tcgcacgtcg gggtccagga gcggctcgcc tacaccgcgc gcgacgaggg ctggtactac
     1741 ctcgaggtgg tgctcgagcg cccgggcgcc ggcccgtaca cgctcagcta cgcgaaatcc
     1801 tgaccttccc gccggccgcg atcggccccc ggcctctcac catggacctg cccgaccgcc
     1861 tcatccgccg gggcacccca tcccaccacc cactgccgat cctatccacg aaaatcgcac
     1921 gcgtgtgtgc caacttcgta acaaaagcgt ggcatgcccg agccaggccg ggagcgcgct
     1981 cgaccgcgcc gcgcgccagt cagtgagccg gcgcgctact ggttctcgag cagctcgtcg
     2041 tcggcgacgg cgcgcagcac gcgcgccggg ctcccgacga cgaccattcg cggcggcacg
     2101 tccttcgtca ccacggcgcc ggcaccgacg aacgcctcct ccccgatctc caccgccggg
     2161 cagaggatcg ctcctccgcc gacacgggcg ccgcgacgga tggtgggccc cttcatcagc
     2221 gcgtgtcgcc gctcggtgcg gcccatgaa
//
LOCUS       DASPWO010000196         5186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_451345, whole genome shotgun sequence.
ACCESSION   DASPWO010000196 DASPWO010000000
VERSION     DASPWO010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451345"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VEO00_04355"
     CDS             complement(<1..1006)
                     /locus_tag="VEO00_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57265.1"
                     /translation="MATARRRGIEAAARIADRAAGERVLVFGSVPPEGRDLDLLARGA
                     GRARIAAALQAKGFTRRGDEWVRFASCGAESVEVIPAGRWHLPPAEEASLFGEARPLP
                     GARHLVRPAPAHALLILARRLVAGQGGIDERRLARVADALREDRDAWRPARERAPAWG
                     AGRALALLHRADRGGPRPTTSERAAALAEGFRAAGSGPAVAAIRAAGAVAPRRRRGRV
                     IALSGLDGSGKSAQASGLRDALAALGHDPVVAWTRLAQDPGLDRVALPVKRAVAAMTR
                     ARGRAGAPGSSDLRASWTQADPGGDVRGPRFVLSWTWALFVALRNGTAQRRATRPNVR
                     A"
     gene            complement(1048..2211)
                     /locus_tag="VEO00_04360"
     CDS             complement(1048..2211)
                     /locus_tag="VEO00_04360"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HYU57266.1"
                     /translation="MRVLIVDDGYSRGAVTAVRSLARAGWSVAVGSPGSLGPAAASRH
                     AGARHDVPPAAADPERFLERVRRAIAAGAYDVVLPTGDAELLALSAARDSLAARFPYA
                     AHPVVERALDKLELGRAAAAAGLDVPQTMDPEAAANALGERPVIVKARRHWTAGDGPR
                     PSRLEAAPARGPTAIRSLADAIRAAGGEPFVQPFIAGTLLAYVALVDQEGRVRAELQQ
                     EALRVCPPDAGTSARARTVPVDPRLAGPARRMLAGLGWFGVAELQFVRDDAGRTYLVD
                     LNGRVYGSLALAAAAGLDLPAAWLALATGGPVPPLRPAAVGVRYQWLEGDLRRARLER
                     RGGLARDVLGCLAFAPGAAHSLLSVSDPGPAMHRGRAVVAHLLRGRAGSRRAG"
     gene            complement(2237..3718)
                     /locus_tag="VEO00_04365"
     CDS             complement(2237..3718)
                     /locus_tag="VEO00_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HYU57267.1"
                     /translation="MDEPNLTEAIEVGTSRRILRNTAYRAVADVGSKLVSIVLYVVMA
                     RRLGQSQFGVFTFGLAFVTLVSALGELGQDQILVREVARDRRKLSSYFGNTLALRVAV
                     TLPVLIVVLAIGAAIGFDGRKLLVVAFLGAGVIVELGISTVIGSFQAFERLEFAPLVL
                     ITQRVLTTAVAVIAIAFGADVVAVALIYLGSALLAFFVGLALLRSKVVRPKADIDVRR
                     WWPLMRAALPLGIAGISATILFRIDTVMLQAYKPDAVVGAYGAAYRLFETTLFLSWSV
                     GAAIYPVMSRLTRTTDPPVALVFERGLKLAIALTLPLAVGAAVLANGVLATLYGNEFL
                     DAAPALRLLAPAIATFPFAFIAGQLLVSQDRQAAMTITYLVVTVENIALNLYLIPRFS
                     LDGAAFGTSLSEVLVVTGLLAFCFARIGRIAWGRAFAGPVVASALAGAAMFLLRDRFL
                     PAAAAGALVYGAALLAVERLAFPGEFTALLGLVRREERVPPAS"
     gene            complement(3747..>5186)
                     /locus_tag="VEO00_04370"
     CDS             complement(3747..>5186)
                     /locus_tag="VEO00_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYU57268.1"
                     /translation="RRRRTLAVAGGLVRDLGPGGTIVKSTEVGTFDWWLRAGRRRTPP
                     EGLPAFFFPDGACMIRRDAFLEVGGFYEPFFLSDIDGLDLATRLLARGWDVRYYPQAL
                     FDHLKIRSGRQVNTADRRLYMRVRNGLWYFWLHFPPWVAARRMTAYLAYELVECVYRG
                     APGAWIGGIRAAWRDRERVRPDRRPLPRAVLRRAEMNRGRTHVRLLVEQARRRVPLGG
                     WTERPPVVLMYHGFGSRTPDQDPHNLFVPLEEFDWQLEYLARRLVPIDLATYVAGWHL
                     RRWPRRSVLVTMDDGYRSTLEGAARLARYGIPAVVFVPPGILGQTTSWMPEMPGERIL
                     SADELREIGALGIELGVHGMDHRTLTGLDPAELRRQVAESRTALADVTGSLPTAFAYP
                     EGEFDAAAVDAVRAAGYTLAFSVGTDGGRFAIPRMDVNGRDTHRTFAIKTAGSLERLI
                     RAADRVPQLRYLGGRIAGKGPQPSRRGPA"
BASE COUNT          753 a         1987 c         1896 g          550 t
ORIGIN      
        1 cggcacggac gttgggccgc gtcgcccggc gctgggccgt cccgttgcgc aacgccacga
       61 acagcgccca tgtccaggag agcacgaacc gcgggccccg gacatccccg cctggatcgg
      121 cctgcgtcca cgaggctcgc aggtcggacg accccggcgc gccggcacgt ccccgggccc
      181 gggtcatcgc ggcgacggcg cgcttcaccg ggagcgccac ccggtcgagc ccggggtcct
      241 gggccaggcg ggtccaggcc accaccgggt cgtggccgag cgcggccagg gcatccctca
      301 ggcccgaggc ctgggcgctc ttgccggatc cgtcgagccc cgacagcgcg atcacccggc
      361 cgcgcctgcg gcgcggggcc acggcgcccg ccgcgcggat ggcggccacc gcgggaccgc
      421 ttccggccgc gcgaaagccc tcggcgagcg ccgccgctcg ctccgaggtg gtcggccgcg
      481 gccctccccg gtcggcccga tggagcaggg cgagggccct gccggcaccc caggcggggg
      541 cgcgttcccg cgcgggcctc caggcgtccc ggtcctcccg gagggcatcg gcgacccggg
      601 ccagccgccg ctcgtcgatg ccgccctgcc ccgcgaccag gcgccgggcc agtatcagca
      661 gcgcatgggc cggggctggg cggaccaggt ggcgtgcgcc gggcagcggg cgcgcctccc
      721 cgaacaggct cgcttcctcg gcgggcggga ggtgccagcg gccggccggg atcacctcga
      781 ccgattcggc gccgcacgag gcgaaacgga cccactcgtc tccgcggcgg gtgaagccct
      841 tcgcctgcag ggcggcggcg atgcgggcac gcccggcgcc gcgtgccagc aggtcgaggt
      901 cccggccctc gggcgggacc gagccgaaca ccaggacgcg ctccccggcg gcgcggtcgg
      961 cgatccgcgc tgccgcctcg atcccgcgtc gtcgtgccgt tgccacgggc gacaccccga
     1021 tcctcggtcc gctgccggcc attatcgtca gccggcccgg cgcgacccgg ctcggccgcg
     1081 caggaggtgg gcgacgacgg cccgcccccg gtgcatggcg gggccggggt ccgataccga
     1141 caacagggag tgcgccgcgc ccggcgcgaa ggccaggcac cccagcacgt cccgggccag
     1201 gccgccccgg cgctcgaggc gggcacggcg aaggtcgccc tccagccatt ggtaccggac
     1261 gccgacggcc gcgggccgga gaggcggaac gggtccgccg gtggccaggg cgagccaggc
     1321 tgccggaagg tcgaggccgg cggcggcggc cagggccagc gaaccgtaca cgcggccgtt
     1381 caggtcgacc aggtacgtcc ggccggcgtc gtcccgcacg aactgcagct cggccacccc
     1441 gaaccagccc agtccggcca gcatccgccg ggccggcccg gcgagccgcg gatcgaccgg
     1501 caccgtccgc gcccgggcac tggtcccggc gtcgggcgga cacacccgaa gcgcctcctg
     1561 ctgcagttcg gcccggaccc ggccctcttg gtccaccagc gccacgtagg cgaggagggt
     1621 gccggcgatg aacggctgca cgaacggctc gccgcccgcg gcccggatgg cgtccgccag
     1681 gctccggatg gcggtggggc cccgcgcggg cgcggcctcg agccgggagg gccggggccc
     1741 gtcgcccgcc gtccagtgcc ggcgggcctt gacgatcacg ggacgctccc ccagtgcgtt
     1801 ggccgccgcc tccgggtcca tcgtctgcgg gacgtccagg cccgccgccg ccgccgcccg
     1861 cccgagctcc agcttgtcga gcgcccgctc gaccacgggg tgcgcggcgt aggggaagcg
     1921 agcggccagg gaatcgcgcg ccgcggacag cgcgagcagc tccgcgtccc cggtcgggag
     1981 cacgacgtcg tacgccccag cggcgatcgc ccggcggacg cgctcgagga accgctccgg
     2041 gtccgccgcc gccggcggaa cgtcatgccg cgcgccggcg tgacgggagg ccgccgcggg
     2101 gccgagactt cccggggacc ccaccgccac gctccacccg gcacgcgcca gggagcgaac
     2161 ggccgtcacc gcgccgcggc tgtacccgtc gtccacgatc aggacccgca cgtcacctcc
     2221 tcccgcggcc gcgggctcag ctcgccggcg gcacccgctc ctcccgccgc accaggccca
     2281 ggagcgcggt gaactcgccg gggaaggcca gccgttccac cgccagcagc gccgcgccgt
     2341 acacgagcgc gccggccgcg gcggccggga ggaaccggtc gcgcagcagg aacatggccg
     2401 ccccggccag ggccgacgcc accacgggcc cggcgaaggc gcggccccag gcgatccgcc
     2461 cgatccgcgc gaagcagaag gccagcaacc cggtcaccac cagcacctcg gacaacgagg
     2521 tcccgaaggc ggcgccgtcg agggagaacc gcgggatcag gtacaggttc agcgcgatgt
     2581 tctccacggt gaccaccagg taggtgatcg tcatcgccgc ctggcggtcc tgcgagacga
     2641 gcagctggcc ggcgatgaac gcgaagggga acgtcgcgat cgccggagcc agcagacgca
     2701 gcgcgggcgc ggcgtcgagg aactcgttgc cgtacagggt tgcgaggacg ccgttcgcca
     2761 gcacggccgc gccgacagcg agcggcagcg tcagcgcgat cgccagcttc aggccccgct
     2821 cgaacaccag cgccaccggc gggtcggtcg tccgggtcag ccgcgacatc accgggtaga
     2881 tggccgctcc gacgctccag gacaggaaca gcgtcgtctc gaacagccgg taggcggcac
     2941 cgtaggcgcc gacgaccgcg tcgggcttgt acgcctgaag catcacggtg tcgatccgga
     3001 agaggatggt cgcgctgatc ccggcgatgc cgagcggcag cgccgcccgc atcagcggcc
     3061 accaccggcg aacgtcgatg tcggccttcg gacgcaccac cttgctccgc agcagcgcca
     3121 ggccgacgaa gaacgccagg agggcgctcc ccaggtagat cagcgccacg gccacgacgt
     3181 cggcgccgaa cgcgatcgcg atgaccgcga cggccgttgt gagcacgcgc tgggtgatca
     3241 gcaccagtgg ggcgaactcc agccgctcga aggcctggaa cgagccgatc acggtgctga
     3301 tccccagctc cacgatcacc ccggccccga ggaacgccac gaccagcagc ttgcgaccgt
     3361 cgaagccgat cgcggcaccg atcgccagca cgacgatcag cacggggagg gtcacggcca
     3421 cgcgaagagc gagcgtgttc ccgaagtagc tcgacagctt gcgccggtcg cgcgccacct
     3481 cgcgcacgag gatctggtcc tgtcccagct ccccgagcgc ggacacgagc gtgacgaagg
     3541 cgagcccgaa ggtgaacacc ccgaattgcg actggccgag gcgccgggcc atcaccacgt
     3601 agaggacgat cgacacgagc ttcgacccca cgtcggcgac ggcccggtag gccgtgttcc
     3661 gcaggatgcg ccgcgaggtc ccgacctcga tcgcctcggt caggttcggc tcgtccacgg
     3721 cgactcccct cgagcaggcg acccgcctac gcgggccctc gcctcgaggg ctgcgggccc
     3781 ttgcccgcga tccgcccacc gaggtatctc agctgcggga cccggtcggc ggcacggatc
     3841 aggcgctcga gggagccggc cgtcttgatc gcgaacgtgc ggtgggtatc ccgcccgttg
     3901 acgtccatcc gcgggatcgc gaaccgtccc ccgtcggtcc ccaccgagaa ggccagcgtg
     3961 tatcccgccg cccgcaccgc gtccaccgcg gccgcgtcga actcgccctc cgggtacgcg
     4021 aacgccgtcg ggaggctgcc ggtcacatcg gccagcgccg tccgggactc cgcgacctgg
     4081 cggcggagct cggccgggtc cagcccggtg agcgtgcggt gatccatgcc gtggacgccg
     4141 agctcgattc cgagcgcgcc gatctcgcgg agctcgtcgg ccgacaggat gcgctcgccg
     4201 ggcatctccg gcatccagga cgtggtctgt ccgaggatcc cgggcggcac gaacaccacg
     4261 gccgggatcc cgtaccgagc caggcgcgcc gccccctcca gggtggatcg gtagccgtcg
     4321 tccatcgtga cgaggacgga ccgcctcggc cagcggcgaa ggtgccaccc tgccacgtag
     4381 gtggccaggt cgatcgggac gaggcggcgg gcgaggtact cgagctgcca gtcgaactcc
     4441 tcgagcggca cgaacaggtt gtgcggatcc tggtcgggcg tgcgcgaccc gaacccgtgg
     4501 tacatcagga cgaccggcgg acgttccgtc catccgccca gcggcacgcg gcggcgggcc
     4561 tgctccacca gcaggcggac gtgcgttcgt ccccggttca tctccgcccg ccgaagcacc
     4621 gcgcgaggga gaggccggcg gtccggccgg acccgctcgc ggtcccgcca ggccgcgcgg
     4681 atcccgccga tccaggcgcc gggcgccccg cggtacacgc actcgacgag ctcgtaggcg
     4741 aggtaggcgg tcatccggcg agccgcgacc cacggtggga agtgcagcca gaagtaccag
     4801 agcccgttgc gcacgcgcat gtagagtcgc cggtcggccg tgttcacctg ccggcccgag
     4861 cggatcttca ggtggtcgaa cagggcctgg gggtagtagc gaacgtccca gccccgagcg
     4921 aggagccgtg tcgccaggtc cagaccgtcg atgtcggaga ggaagaacgg ctcgtagaac
     4981 ccgccgacct cgaggaaggc gtcgcggcgg atcatgcagg ccccgtcggg gaagaagaag
     5041 gcgggcaggc cctcgggcgg cgtccgtctc cgtcccgccc gaagccacca gtcgaacgtc
     5101 ccgacctcgg tggacttcac gatcgtgccg ccgggaccga gatcccggac caggccaccg
     5161 gccacggcga gggttcggcg gcgacg
//
LOCUS       DASPWO010000197         4889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_797216, whole genome shotgun sequence.
ACCESSION   DASPWO010000197 DASPWO010000000
VERSION     DASPWO010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797216"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VEO00_04375"
     CDS             <1..202
                     /locus_tag="VEO00_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYU57269.1"
                     /translation="GVDKRRYMSTMFGEADLDVFQRLRCAFDPHGLANPGKVMPTPRL
                     CGEVPGPYRQHPLEAAGVIERF"
     gene            202..1380
                     /locus_tag="VEO00_04380"
     CDS             202..1380
                     /locus_tag="VEO00_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYU57270.1"
                     /translation="MVRSSVRVEEPPTSPEAAEILREAAARSLSVRFRGGGTKFDWGN
                     ATPEPDLIVSTHGLNRIVEHNAADLTAVLEAGVRLADAQAAFAESGQMLAIDPPLGPD
                     DEATIGGIVASGDSGPLRHRYGAARDLLVGMTVVLSDGTIAKSGGRVIKNVAGYDLAK
                     LFTGSFGTLGMIVQVAVRLHPKPREQMTVIGSTDDPDALGRAAAALAPAPLEFESLDA
                     DWDQSSGRILARCAGTATRPRIEAASRLLPEAMAGSRYTSSIERDEDDELIWSSVRSR
                     SLVGADGVLVKVSGLPSEVPRLIRTVTRLRGHLVARAGLGLAWVWLPPTNDGATIAAI
                     EELRRDLAPYPCVVLEAPPGVREGIDVWGPPDGSIELMRRIKERFDPAGICNPGVFVV
                     "
     gene            1382..2725
                     /locus_tag="VEO00_04385"
     CDS             1382..2725
                     /locus_tag="VEO00_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HYU57271.1"
                     /translation="MDRAFDLHRPPDQDLMDVCVHCGFCLPTCPTYVLWGEEMDSPRG
                     RISLMHHGLEGGSDMAEMAIHFDRCLGCMACVTACPSGVRYDRLIEATRPQVERNAPR
                     SRRERAFRRVLFEVVTHPGRLRALAPVLSAARAFGLVGLARTRAAGRFPRLRAIARLA
                     PRVAAGPSARRLPEITPAVGPVRGRVALLQGCVQRVFFHDVNAATVRVLASEGFEVDA
                     PRLPRCCGALQLHSGDERGALDLARRSIAVFERFDHVVVNAAGCGSAMKDYGHLLADD
                     RRWGGRAAAFSAKVRDVTELLAEAGTVAERNPVPVRLAYHDACHLAHAQGVRRQPRDL
                     LREVPGLELVEPAEWEICCGSAGVYNILQPDAADELGRRKARHVMATGAEVIAAGNPG
                     CALQIRTHLEALGHPIRIVHPVEILDASIRGADLLAPGAPGDGLRASGGGRTSPG"
     gene            complement(2688..4631)
                     /locus_tag="VEO00_04390"
     CDS             complement(2688..4631)
                     /locus_tag="VEO00_04390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYU57272.1"
                     /translation="MAGMVPQDVYALTGVADPRISPDERWVAYGVWSVDAERNEYRGA
                     IWVTPVDGSSPPRRVTAGTKRDANPRWSPDGARLAFTSNRDTKAAQLYLLPMDGGDAR
                     RLTDLPEDVADPRWSPDGTRLLFASRVRDEPYAEEDDARRRPRRFRRLRYRHDNIGWI
                     GDRRQHLFVVPADGSGKPRQITDGDFDDADGFWSPDGSTIAFSSSRDEDWDLDPVSDI
                     YVVDAEGGDPTKLTHGDGSCDGPAWSPDGSRIAYRFVPGLYDIPRHSQIAVIPSSGGP
                     RTVLTAALDRTCNLYPPGREPIWRGDRIVFAVEDRGNAHLYQVLADGSAPPSPLMDGE
                     TVVTGWDAVGETVVYSAGRPTALDELFVADRPRTSVGDAFAHGRELVEPEPFTAVSWD
                     GTEVDAWIVRPAGFVEGTRYPVLLSIHGGPFTRYGNRLFDEFQVYAGAGYAVVYSNPR
                     GSSGYTEAWGRAIRGPVDGGPGWGTLDYQDVMAAIDTALQRFDFCDPDRVGVLGGSYG
                     GFMTSWIVSHTDRFRAACSERAVNQWVSFYGASDIGPWFPKGYMGGFLFEDFDAWVAY
                     SPATYAQNIRTPLLILHSEDDLRCPIDQGEQLFTTLRLLKREVEFVRFPKESHELSRS
                     GNPVHRVMRFEVILDWFDRHLKP"
BASE COUNT          709 a         1684 c         1752 g          744 t
ORIGIN      
        1 cggggtcgac aagcgccggt acatgtcgac gatgttcggc gaggccgacc tggacgtgtt
       61 ccagcggctc cgctgcgcgt tcgatccgca cgggctggcc aatcccggca aggtcatgcc
      121 cacgccgagg ctgtgcggcg aggtccccgg cccctaccgg cagcatccgc tggaggcggc
      181 cggtgtgatc gagcggttct gatggttcgg tcgtccgtgc gggtggagga gcctccgact
      241 tcccccgagg ctgccgagat cctgcgcgag gcggcggcgc gatccctgag cgttcggttc
      301 cggggcggcg ggacgaagtt cgactggggc aacgccaccc ccgagccgga cctcatcgtg
      361 tccacccacg gcctcaaccg catcgtggag cacaacgcgg cggacctcac tgcggtgctc
      421 gaggcgggcg tgcggctggc cgacgcccag gcggccttcg ccgagtcagg ccagatgctg
      481 gcgatcgacc cgccgctcgg tccggacgac gaggcgacga tcggtggcat cgtggcctcg
      541 ggggactcgg gtccgcttcg acaccgatat ggcgccgcgc gcgacctgtt ggtcgggatg
      601 accgtggtgc tgagcgacgg aacgatcgcc aaatccggtg gcagggtgat caagaacgtg
      661 gcgggctacg atctggcgaa gctcttcaca ggttcgttcg ggacccttgg gatgatcgtt
      721 caggtcgccg tgaggctgca ccccaagccg cgagaacaga tgaccgtgat cgggtccacg
      781 gatgatcccg acgcacttgg gagggccgcc gctgccctgg cgcctgcccc gctcgagttc
      841 gagtccctgg acgccgactg ggaccagtcg agcggacgga tcctggcccg gtgcgcgggg
      901 acggcgactc ggccccggat cgaagcggcg tcccgcctcc tgccggaggc catggccggc
      961 tcccggtaca cctcctcgat cgaacgggac gaggacgacg agctcatctg gagctcggtc
     1021 cgttctcgat cccttgttgg cgcggatggc gtcctggtaa aggtctccgg cctgccatcc
     1081 gaagtgcctc gcctgatcag gaccgtgacg cggctccgtg gtcacctggt ggcaagggcg
     1141 ggactggggc tggcctgggt ctggctcccg cccaccaacg acggcgcgac gatcgcggcc
     1201 atcgaagagc ttcgccggga cctggcgccc tacccgtgcg tcgtgctcga ggcgccgccc
     1261 ggggtccgcg aagggatcga cgtgtgggga ccgccggacg gctccatcga gctgatgcgg
     1321 cggatcaagg aacggttcga tcccgccggg atctgcaacc ccggcgtctt cgtggtctga
     1381 catggatcgg gccttcgacc tccaccggcc gcccgaccag gacctcatgg acgtgtgcgt
     1441 ccactgcggg ttctgcctgc cgacgtgccc gacctacgtc ctgtggggcg aggagatgga
     1501 ctccccccgg gggcggatct ccctcatgca ccacgggctc gaagggggca gcgacatggc
     1561 cgaaatggcg atccatttcg accgctgcct ggggtgcatg gcatgtgtga cggcgtgccc
     1621 gtcgggggtg cggtacgacc gcctgatcga agcgacccgc ccgcaggtcg aacggaacgc
     1681 tccgcgctcg cggcgggagc gagccttccg ccgggtgctg ttcgaagtcg tcacgcatcc
     1741 ggggcgcctc cgcgcgctgg cgccggtgct ctccgcggcg cgcgcgttcg ggctcgtcgg
     1801 actggctcga accagggcgg ctggccggtt cccgaggctc cgggccatcg ctcgactcgc
     1861 tcccagggtc gccgccggcc cttcggcccg ccggctcccg gagatcacac cggcggtggg
     1921 cccggtcagg ggccgggtcg cgctcctgca gggatgcgtc cagcgggtgt tcttccacga
     1981 cgtgaacgcg gcgacggttc gggtgctcgc ctcggagggg ttcgaggtcg acgcgccgcg
     2041 cctgccgcga tgttgcggcg ccctgcagct ccattcgggc gatgaacggg gtgcgctgga
     2101 cctcgcccgg cgatcgatcg ccgtgttcga acgattcgac catgtggtcg tgaatgcggc
     2161 cggttgcggt tccgcgatga aggactacgg gcacctgctc gccgacgacc ggcggtgggg
     2221 cggccgggcc gcggcgttct cggcgaaggt ccgagacgtc accgaactgc tggccgaggc
     2281 gggtacggtg gcggagcgga atccggtccc ggtcaggctg gcctatcacg acgcgtgcca
     2341 cctggcccac gcgcagggag tccggcgcca gccgcgcgac ctgctgcggg aggttccggg
     2401 cctcgagctc gtcgagcccg cggaatggga gatctgctgc ggctcagccg gcgtctacaa
     2461 catcctgcag ccggacgcgg cagacgagct gggacggcgc aaggcgcgac acgtcatggc
     2521 caccggagcc gaggtcatcg ccgccgggaa tcccgggtgc gcgctgcaga tccggaccca
     2581 cctggaagcc ctcggtcacc cgatccggat cgtccacccg gtggagatcc tcgacgcctc
     2641 gatccggggc gccgatctgc tggcaccggg ggcgcccggc gacggcctca gggcttcagg
     2701 tggcggtcga accagtccag gatgacctcg aagcgcatca cccggtgcac gggattgccc
     2761 gaccgcgaca gctcgtggct ctccttcggg aaccggacga attccacctc ccgcttcagg
     2821 aggcgcagcg tcgtgaacag ctgctcaccc tggtcgatcg ggcaccgcag gtcgtcctcc
     2881 gaatgcagga tcagcagcgg ggtccggatg ttctgcgcgt acgtcgcggg cgagtacgct
     2941 acccacgcgt cgaagtcctc gaacaggaaa ccgcccatat agcccttcgg gaaccagggg
     3001 ccgatgtcgc tcgccccgta gaacgagacc cactggttga ccgcacgctc cgagcaggcg
     3061 gctctgaacc ggtcggtgtg ggaaacgatc caggacgtca tgaacccgcc gtacgagccg
     3121 cccaacaccc ccacccggtc ggggtcgcag aagtcgaacc gctgaagcgc cgtgtcgatg
     3181 gcggccatga cgtcctggta atcgagggtg ccccatccgg gaccgccgtc gaccggtccg
     3241 cggatggccc gcccccaggc ttcggtgtat ccggaggagc ctcgggggtt cgagtagacc
     3301 accgcgtacc cggcgcccgc gtacacctgg aactcgtcga acagtcgatt cccgtaccgg
     3361 gtgaacggcc cgccgtggat gctgagcagg acggggtacc gcgtgccctc cacgaaccca
     3421 gccggccgca cgatccaggc gtcgacctcg gttccgtccc aggagactgc ggtgaatggc
     3481 tccggctcga ccagctcccg gccgtgggcg aacgcgtctc ccacgctcgt ccggggtcga
     3541 tcggccacga acaattcgtc cagggccgtc ggccggcccg ccgaatagac gacggtctcg
     3601 ccgaccgcat cccacccggt gacgacggtc tcgccgtcca tcagcggcga ggggggcgcc
     3661 gagccgtcgg cgaggacctg gtagagatgg gcgttcccgc ggtcctcgac cgcgaagacg
     3721 atccgatcgc cgcgccagat cggctcgcgt cccggcgggt agaggttgca ggttcgatcg
     3781 agcgcggccg tgagcaccgt ccggggcccg ccgctggaag ggatcaccgc gatctgggag
     3841 tggcgcggga tgtcgtagag accggggacg aatcggtagg cgatgcgcga gccgtcgggt
     3901 gaccaggcgg gcccgtcaca cgagccgtcc ccgtgagtca gcttcgtcgg atcgccaccc
     3961 tcggcgtcca ccacgtagat gtccgagacg gggtcgaggt cccaatcctc gtcccgggac
     4021 gatgagaacg cgatcgtgct gccgtcgggt gaccagaagc cgtcggcgtc gtcgaagtcg
     4081 ccgtcggtga tctgccgagg cttccccgag ccgtcggccg gcactacgaa caggtgctgc
     4141 cgccgatccc cgatccatcc gatgttgtcg tgtcggtatc gaagccggcg gaatcgccgg
     4201 ggccggcgcc tggcgtcgtc ctcttcggcg tagggctcgt cccggacccg agaggcgaac
     4261 agcagccggg tcccgtcggg cgaccagcgc gggtcggcca cgtcctcggg gagatcggtg
     4321 agccttcgcg catctcctcc gtccatcggg agcaggtaga gctgcgcggc cttggtgtcg
     4381 cggttcgagg tgaaggccag tcgggcgccg tcgggcgacc atcgggggtt cgcgtcgcgc
     4441 ttcgtcccgg cggtcacccg gcgggggggc gagcttccat ccaccggggt gacccagatc
     4501 gcgccgcggt actcgttgcg ctccgcatcg accgaccaca cgccgtaggc cacccaccgc
     4561 tcgtccggcg agatgcgggg gtcggccacg ccggtcaacg cgtacacatc ctgcgggacc
     4621 atcccggcca tgcgtggctc ctctcgggcc cgaacgcttc tgctcgaggt ccgggaaccc
     4681 tatccgttca ggaaggcgtc gaaccccgtg accacgctgg gatggtcgaa ctcgagggcg
     4741 ggagccacgg cgacgggaat gctgacgccc tcgggccgat cgatcaaacg acgggtgtca
     4801 ggtcccctcg acgtcgtccg gcctcacgaa cgggcgacgg cggttggctg tcccgcgccg
     4861 gagtcgcctt cgcgcttcga gttcggaat
//
LOCUS       DASPWO010000198         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_29700, whole genome shotgun sequence.
ACCESSION   DASPWO010000198 DASPWO010000000
VERSION     DASPWO010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29700"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="VEO00_04395"
     CDS             <1..1383
                     /locus_tag="VEO00_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYU57273.1"
                     /translation="KVGTADLDRALAAHLCRCTGWRTVYDAIAHAAEPTPTAPARRDP
                     DAAARRAALEGGAPQRVGADIPLGGGGFADDLAPREALVAVPLPPGSDAPSREAAGLR
                     WVLADTLLEARGLAAKVQGRRTTIDERSPLPLPPLPGGGVRLATGWVEPAYLEPDASW
                     CEPGREPATPLANGGAFGGKESSPVAAAARQLADGTGRTVRVVLSREDVVRIGPKRPP
                     MAASAIAADGRVTVAGSVAGGGLSAFLSPVAGWPYRIGVEADWELVDVPGPPVADHLR
                     AVGLAEHAVLVEGALSEAGVDRSERSRDERAAAALLDTCAPGPGESGALAGARVDIDP
                     ASGAVERLHVRVAAGDPLDEVVLRSYATGAAHMALGWVLSEGIAVDPATGEAHDLTIR
                     SFGVIRAKDTPPIEIEIVDDPGPPQARASDAVFAAVAAATWNAVTDAEGARPESFPAR
                     ETRAARRLRR"
     gene            complement(1388..2026)
                     /locus_tag="VEO00_04400"
     CDS             complement(1388..2026)
                     /locus_tag="VEO00_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57274.1"
                     /translation="MDDTKWERYAGIAGFLFVILVLVVSFIPGAPPKPDDVGDIAKYF
                     KDHDGAIQAAAFLNGFAGLAFLVFVTAVSGMIRRAEGAPRLSVVAFGGGAVALVFAFA
                     NAAILSAAAITDSGSQGTYVLSLTFTGVGTFGLIALAGATAVAAFQTRMLPNWVAWIS
                     AATAVVAIPGASIASTSRDAAFFLSFIGFLVFVVWVLSVSFVLFRPQAAAAK"
     gene            complement(2082..>2654)
                     /locus_tag="VEO00_04405"
     CDS             complement(2082..>2654)
                     /locus_tag="VEO00_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57275.1"
                     /translation="PPATSDSAAKIGKFFADNDYALKCGAFISGLASIPFLVFLGVLY
                     ARLRGAEGDHGRLSLVMFGGGVLSAALILVAFAVFGAIAISVDEIGGGGTKFFYILAN
                     GLTGMAGLALVVVAGATALVTFRTQALPAWLGWLSVASAVLWIPGGLVVASDSDAAFV
                     INIIAFPLFLVWVLATSIVLFRTQTAATTR"
BASE COUNT          406 a          951 c          961 g          336 t
ORIGIN      
        1 aaggtgggca ccgccgacct cgaccgggcc ctggcggcgc acctctgtcg ttgcaccggg
       61 tggcgcacgg tgtacgacgc gatcgcacac gcggccgagc cgacaccaac cgcgcctgcc
      121 cgacgtgatc ccgacgccgc cgcccgtcgc gccgccctgg aaggcggggc tccgcagcgg
      181 gtcggggccg acatcccact cggcggcggc gggttcgccg acgacctcgc gccgcgcgag
      241 gcgctggtgg cggtgccgct gccgccgggc tcggacgcgc cgtcccggga ggcggcgggc
      301 ctgcgctggg tgctggccga cacgctgctg gaagcgcgag gcctcgccgc caaggtccag
      361 ggtcggcgca ccaccatcga cgagcggtcg cccctgccac tgccgccgct gccgggcgga
      421 ggagtgcgcc tcgccaccgg ctgggtggag ccggcgtacc tcgagccgga cgcgtcgtgg
      481 tgcgagcccg gccgcgagcc ggccacgccg ctggccaacg gcggcgcctt cggcggcaag
      541 gagtcgtcac cggtggcggc ggccgcgcgc cagctcgccg acggcaccgg gcgaacggtg
      601 cgggtggtgc tgtcgcgaga ggacgtcgtc cgcatcgggc ccaagcggcc gccgatggcg
      661 gcgagcgcga tcgcggccga cggccgggtc acggtcgcgg gctcggtcgc gggcggcggg
      721 ctgtcggcat tcctctcacc ggtcgcgggt tggccctacc gcatcggtgt cgaggccgac
      781 tgggagctcg tcgacgtccc cgggccgccg gtggcagacc acctccgagc ggtgggtctc
      841 gccgagcacg cggtcctcgt cgagggcgcg ctgtccgagg cgggcgtcga ccggagtgag
      901 cgctcccgtg acgagcgcgc cgccgcagcc ctcctcgaca cctgcgcgcc gggaccgggc
      961 gagagtggcg cgctcgccgg cgcccgggtc gacatcgatc cggcatcggg agccgtcgaa
     1021 cgcctccatg tgcgggtggc ggccggcgat cctctcgacg aggtggtgct gcgctcctac
     1081 gcgaccggcg ccgcccacat ggccctgggc tgggtgctct cggagggcat cgcggtcgac
     1141 cccgcgaccg gcgaggcgca cgacctgacc atccgttcct tcggggtcat ccgggccaag
     1201 gacacgcccc cgatcgagat cgagatcgtc gacgaccccg gtccaccgca agcgcgggcg
     1261 tcggacgcgg tgttcgcggc ggtcgccgcc gccacctgga acgcggtgac ggacgcggag
     1321 ggcgcgcggc ccgagtcgtt cccggcgagg gagacccgag ccgcgcgccg actccgccgc
     1381 tagagaccta cttcgctgcg gccgcctggg gacggaacag cacgaagctg accgagagca
     1441 cccagaccac gaataccagg aacccgatga agctgaggaa gaacgcggca tccctcgacg
     1501 tcgaggcgat ggaggcgccg gggatcgcca ccaccgcggt cgcggcgctg atccaggcga
     1561 cccagttcgg gagcatccgc gtctggaagg cagccacggc ggtcgcaccc gcgagggcga
     1621 tgaggccgaa ggtgcccaca cccgtgaatg tgaggctgag cacatacgtg ccctggcttc
     1681 cgctgtcggt gatcgccgct gcgctcagga tcgccgcatt cgcgaaggcg aagaccagcg
     1741 ccacggcgcc gcccccgaac gccaccacgc tcagtcgtgg ggcgccttcg gcgcgccgaa
     1801 tcatccccga cacggcggtc acgaacacga ggaacgcgag tccggcgaag ccgttgagaa
     1861 aggctgccgc ctggatcgct ccgtcgtggt ccttgaagta cttcgcgatg tcgccgacgt
     1921 catccggctt tggtggcgct ccgggaatga acgagaccac cagcacgagg atcacgaaca
     1981 agaacccggc tatccccgcg taccgctccc atttcgtgtc gtccatgaca gattccccct
     2041 gtggtcagcc cgccgtagcg gttcggttgc tccgccggtg attacctcgt cgtcgccgcc
     2101 gtctgcgtgc ggaacagcac gatgctggtc gcgaggaccc agacgaggaa caacgggaag
     2161 gcgatgatgt tgatgacgaa ggcagcgtcg ctgtctgacg cgacgacgag cccgccaggt
     2221 atccacagga cagcactcgc cacgctgagc catccgagcc acgcaggcag cgcctgcgta
     2281 cggaatgtga cgagcgcggt tgcgccggcg acgacgacga gcgcgagccc tgccatgccc
     2341 gtgagcccgt tggcgaggat gtagaagaac ttggtccctc cgcctccgat ctcgtcgacc
     2401 gagatggcga tcgcgccgaa gaccgcgaac gcgacgagga tgagcgcggc cgacaggacc
     2461 ccgccaccga acatcaccag gctcaatcgc ccgtggtccc cctcggcgcc gcggagcctc
     2521 gcgtagagaa ccccgaggaa tacgaggaac gggatcgagg ccagccccga gatgaacgcg
     2581 ccgcacttga gcgcgtagtc gttgtcggcg aagaacttcc cgatcttggc ggccgagtcg
     2641 ctcgttgccg gcgg
//
LOCUS       DASPWO010000199         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_29780, whole genome shotgun sequence.
ACCESSION   DASPWO010000199 DASPWO010000000
VERSION     DASPWO010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29780"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="VEO00_04410"
     CDS             <1..1253
                     /locus_tag="VEO00_04410"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR02692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HYU57276.1"
                     /translation="DFATDAKPEEIVRVLRGWADRQYLVGVRFGTVSAAKDALHLEIT
                     TFRSEAYPEDDRHPQVTFTDDLEADLSRRDFTINAMAVSVPDVRFVDPFGGLADLAHR
                     RLATPLDPHVAFSDDPLRMLRAARFASTLEATPAPDVLTAIREMRQRLAIVSPERIRA
                     ELDKLLLSEKPSRGLEIVVDTGLADAFLPELPALRLEQDPVHRHKDVFRHTLAVIERV
                     ERELTVRLAAMLHDIGKPKTRSFTDEGVQFHHHEVVGARMAEERLRALRYPERVVADV
                     VKLVEMHLRFHTYRLGWSDGAVRRYVRDAGPLLDRLNQLVRADCTTRNPFKARALAAL
                     QDELEERVARLAEEENLARIRPPLDGNQVMAHLGIPPGPLVGEALEHLLELRMDRGPI
                     PEDEAHALLDEWARGKGLSPGRPE"
     gene            1254..>2318
                     /locus_tag="VEO00_04415"
     CDS             1254..>2318
                     /locus_tag="VEO00_04415"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU57277.1"
                     /translation="MADAFPEAGPLGEARPKARHPAVRALSNRPFRLFFIGNLISQTG
                     TFLQSAALAVLVFDLTGTNAAVGLTNAATFLPVLALALVGGELADRFNRRRLFLVTQV
                     VSTIAVAALAALTAAGRVTPGWIYALAGVLGLCYAVAIPTMHSLIPSLVPPADLGPSI
                     GLTSVTFNAARIIGPAVASAAIAGLGFAGAFGLNALSFVPMIVALALIRVTPRAADRP
                     RVSLVEGVRYAWREPAVRGMLLAVASIAFAFDPVMTLAPGFARRVFGHSGAAAPVLMA
                     AFGVGSVLSAAFLGRMFRRPEANVRRGSATLFLLGAGMAGFALSPTFEVGVAMLLVAG
                     LGSLSSVTLFTTGLQQIVSED"
BASE COUNT          269 a          841 c          852 g          356 t
ORIGIN      
        1 tcgacttcgc caccgacgcc aagccggagg agatcgtgcg cgtactgcgc ggatgggcgg
       61 accgccagta cctggtcggc gtgcggttcg ggaccgtctc ggcggcgaag gacgccctcc
      121 acctggagat cacgacgttc cgcagcgagg cctacccgga ggacgaccgc cacccgcagg
      181 tgaccttcac ggacgacctg gaggcggacc tgtcgcggcg cgacttcacg atcaacgcga
      241 tggcggtctc ggttccggac gtccggttcg tggacccgtt cgggggcctg gccgatctgg
      301 cccatcgccg tctggccacg ccgctcgacc cccacgtggc gttctccgac gacccgctgc
      361 ggatgctgcg cgccgcgcgg ttcgcctcga ccctcgaggc gacgccggcg ccggacgtgc
      421 tgaccgcgat ccgcgagatg cgccagcgcc tggccatcgt ctcccccgag cgcatccgag
      481 ccgagctcga caagctgctg ctgtcggaga agccgtcgcg cggcctggag atcgtcgtgg
      541 acacgggact ggcggatgcg ttcctccccg agctcccggc gctgcggctg gagcaggatc
      601 cggtgcaccg gcacaaggac gtgttccgcc acacgctcgc cgtgatcgag cgcgtggagc
      661 gggagctgac cgtccggctg gcagcgatgc tgcacgacat cgggaagccg aagacgcgct
      721 cgttcaccga cgagggcgtg caattccatc accacgaggt ggtgggggcg cggatggccg
      781 aggaacggct gcgggcgctc cggtacccgg agcgcgtcgt cgccgacgtc gtgaagctgg
      841 tcgagatgca cctgcgcttc cacacctacc ggctggggtg gtcggacggc gccgtgcgcc
      901 gctacgtccg cgacgcgggg ccgctgctcg accggctgaa ccagctggtc cgcgcggact
      961 gcaccacgcg caacccgttc aaggctcgcg cgctcgccgc gttgcaggac gagctggagg
     1021 agcgcgtcgc gcgcctcgcc gaggaggaga atctcgcccg gatccgcccg cccctcgacg
     1081 gcaatcaggt catggcccac ctcgggatcc cgccgggtcc gctggtgggc gaggcgctcg
     1141 agcacctgct cgagctgcgg atggaccgcg gcccgatccc ggaggacgag gcccacgcgc
     1201 tcctcgacga gtgggcgcgg ggcaaggggc tctctcccgg aaggccggag taggtggccg
     1261 acgcgtttcc ggaggccggc ccgctcggcg aggcccgccc gaaggcgcgc caccccgccg
     1321 tccgggcgct gtcgaaccgc ccgttccggc tgttcttcat cggcaacctg atctcgcaga
     1381 ccgggacctt cctgcagtcg gcggcgctgg cggtgctggt gttcgacctc accgggacga
     1441 acgccgccgt cggactgacg aacgccgcga ccttcctgcc ggtcctcgcg ctcgcgctgg
     1501 tcggcgggga gctggccgac cgcttcaacc ggcgccggct gttcctggtc acccaggtcg
     1561 tgtccaccat cgccgtcgcg gcgctcgccg ccctgaccgc cgccggccgc gtcacgcccg
     1621 ggtggatcta cgctctggcg ggcgtgctcg gcctctgcta cgcggtcgcc atcccgacga
     1681 tgcactcgct gatcccgagc ctggtgccgc cggccgacct cgggccctcc atcgggctga
     1741 cctcggtgac gttcaacgcc gcccggatca tcggcccggc cgtggcctcg gcggcgatcg
     1801 ccgggctggg cttcgcgggg gcgttcgggc tgaacgcgct gtcgttcgtg ccgatgatcg
     1861 tggcgctggc gctgatccgg gtgacgccgc gtgccgcgga ccgcccgcgc gtgtcgctgg
     1921 tggagggggt gcgctacgcg tggcgcgagc cggccgtgcg cggcatgctg ctcgccgtcg
     1981 cctcgatcgc gttcgcgttc gatcccgtga tgacgctcgc gccgggattc gcgcggcggg
     2041 tgttcggcca ctccggcgcg gcggccccgg tgctgatggc ggccttcggg gtgggctcgg
     2101 tgctgtcggc cgcgttcctc gggcggatgt tccgccgccc ggaggcgaac gtgcgccgcg
     2161 ggtcggcgac gctcttcctg ctcggcgccg gcatggccgg gttcgcgctc agcccgacgt
     2221 tcgaggtcgg cgtggccatg ctgctggtgg ccgggttggg gtcgctctcg tcggtgacgc
     2281 tgttcaccac cggcttgcag cagatcgtct ccgaggac
//
LOCUS       DASPWO010000200         5236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_370793, whole genome shotgun sequence.
ACCESSION   DASPWO010000200 DASPWO010000000
VERSION     DASPWO010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370793"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..873)
                     /gene="rph"
                     /locus_tag="VEO00_04420"
     CDS             complement(133..873)
                     /gene="rph"
                     /locus_tag="VEO00_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HYU57278.1"
                     /translation="MTAPVQALPRSDGRAANDLRPIRIEVGYQEWAEGSVLFEMGKTR
                     VLVAASWEPKVPRWLAGTGRGWVTAEYSMLPRATSQRTPREVQQGRPSGRTQEIQRLI
                     GRSLRAVTHLDRMAEQAITVDCDVLQADGGTRTASVTAGYIALGLAVRRLQERGVLGE
                     DVLADSVAAVSAGIVGEERLLDLCYEEDARAEVDFNVVMTGSGRLVEVQGTAEGLPFG
                     REDLDALLDQSADGIAVLTKIQRDALSV"
     gene            complement(870..1616)
                     /locus_tag="VEO00_04425"
     CDS             complement(870..1616)
                     /locus_tag="VEO00_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYU57279.1"
                     /translation="MDLTALGTSGAWPGAGRAASGYLLRHDGFRVALDLGTGTLSRLQ
                     QLIDLKDLDGIVISHAHYDHFLDLYPLFVARFFHPEELPPIPVLAPNGFFDYFCRLTE
                     DEGKDMPSVFECRELGFGQETDLGPFRIRTWEMTHLVPTIGVRLQADGTSVAYTGDTG
                     PTDEVEALARGTDLLLSEAALQDMNEPPWFHLTARQAGEYARRAEVPSLVLSHFWPTA
                     DRDLSREQAAEVYQGDLTMFEEGDTVEVGS"
     gene            complement(1607..2455)
                     /gene="murI"
                     /locus_tag="VEO00_04430"
     CDS             complement(1607..2455)
                     /gene="murI"
                     /locus_tag="VEO00_04430"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HYU57280.1"
                     /translation="MSDGLGGHLERDDRPIGTFDSGVGGLTVVRAMIDLLPHESVLYF
                     GDTARFPYGQRSLEEIAGFAREIAAYLLSRDVKMLVVACNSIEVAAIEALTEASGVPV
                     AGVVDPGARAAVAATRNGRIGVIGTEATIASGAYERAIARTGADVELLTAACPAFVRF
                     VERGDTTSPALRAHAEQYLAPLAAAGVDTLVLGCTHYPLLAGLLRLVMGPEVTLVSSA
                     EETAKDVYATLVGSGSLAAGPPRHEFVSSGDPELFRALGGMFLGPEVSEVRGATVAGV
                     GSGPWT"
     gene            complement(2452..3384)
                     /locus_tag="VEO00_04435"
     CDS             complement(2452..3384)
                     /locus_tag="VEO00_04435"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HYU57281.1"
                     /translation="MKVASVLDSIGNTPLVGLPRTSPNPSVRLWAKLEGQNPTGSLKD
                     RIARKMVEEAEAAGTLVPGKELLEPTSGNTGIALALVARLKGYRLTVVIPENASDERV
                     RLLELFGAEIVYSPAEKGTNGAIEVAEAMARDDKYFMPFQYGNPANPAAHYEGTGAEI
                     LRDLPDVSHFVAGLGTGGTLMGVGRRLREHDPSVQIVAAEPELGELVYGLRSLDEGFI
                     PPIFDEAILDRKFLVGSSDALRATRQLVEREGIFAGISSGAVVHVARRLAEEIDEGDV
                     VCLLADGGWKYLSTEAWAADLGRAEKNVGETLWW"
     gene            complement(3437..>5236)
                     /locus_tag="VEO00_04440"
     CDS             complement(3437..>5236)
                     /locus_tag="VEO00_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYU57282.1"
                     /translation="QADGKIVVAGSVAGGSLRHFGVARYLPDGSLDPTFDGDGRAVTV
                     FSVYGEEALGVAIQPNGKIVVAGRASNGTINRFALARYNANGSLDTNFNGDGRLTTTF
                     VNNESVVAMAVAILSDGRIVAGGYRSCGLFTCGFAAARYTSNGDPDNMFSGNGQLTTG
                     DSLDLGRDLAVQADGKLVMAGWGNGVFKLVRLTTSGGTDSTFSGDGVQTTDFGAPSEG
                     ANGVVIQSNGRIVAAGFAGDHVALARYTTGGALDTSFSGDGKVTGATGSFTDSAEELA
                     IQDDGKLVVPGRHTPAGDDPRFVLWRLESGGTFDGTFSGDGRATLAFGTGPDIGRAAA
                     IQDDGRLVVAGSAWTGANTDLALARFEGETIVTGANLGITMSDSPDPVTVGSTITYHL
                     TVTNGGPEPATGVTVATTLPAGATKQSATASQGSCSGTTTVTCTLGAIGVAGSATVDI
                     VASTSMAGTATATAVVSGNESDPQPANDSATAQTTVNANANGCTILGTGGDDVIVGSP
                     GNDVVCASTGNDTVSGGGGDDDLRGGAGNDTLNGGADDDILTGGPDSDDLFGNAGMDT
                     LQAQDGVTGETVDGGTEGDTCTADPGDVLVSCP"
BASE COUNT          720 a         1965 c         1807 g          744 t
ORIGIN      
        1 ccgtctcgag gatctcgggc acctcgacgt ccctcgcggt cagccacgac accggccagt
       61 ccccgcagat ctccaggatc tcgcgaacct tccccgggtt gcgggtggcc aggacgatcc
      121 ggtcggggaa cgtcaaaccg agagggcgtc ccgctggatc ttcgtcagca ccgcgatgcc
      181 gtcggccgac tggtcgagca gcgcgtcgag gtcctcgcgg ccaaagggga gcccctcggc
      241 ggtcccctga acctcgacca ggcgtccgct gccggtcatg accacgttga agtcgacctc
      301 ggcccgggcg tcctcctcgt agcagaggtc gagcaggcgc tcctcgccga cgatgcccgc
      361 ggacaccgcg gccaccgagt cggccaggac gtcctcgccg agcaccccgc gctcctgcaa
      421 gcgccggacg gccaggccga gcgcgatgta gccggcggtg accgaggcgg tgcgcgtgcc
      481 gccgtcggcc tgcagcacgt cgcagtcgac cgtgatcgcc tgctccgcca tccggtcgag
      541 gtgcgtcacc gcccgtagcg accgtccgat cagccgctgg atctcctggg tgcgcccgga
      601 cgggcggccc tgctggacct cgcgcggcgt gcgctgcgac gtcgcgcggg gcagcatcga
      661 gtactcggcc gtcacccagc cgcggccggt cccggcgagc caccggggga ccttcggctc
      721 ccacgaggcg gccaccagca cgcgcgtctt ccccatctcg aacaggaccg agccctccgc
      781 ccactcctgg tacccgacct cgatccggat cgggcgcagg tcgttcgccg cgcggccgtc
      841 cgatcgcggg agcgcctgca ccggcgccgt cacgatccga cctccaccgt gtcgccttcc
      901 tcgaacatcg tcaggtcgcc ctggtacacc tcggccgcct gctcccggga caggtcgcgg
      961 tcggcggtcg gccagaaatg cgagaggacg aggctcggga cctccgcgcg ccgcgcgtac
     1021 tctccggcct ggcgagcggt gaggtggaac cacggcggct cattcatgtc ctgcaaggcc
     1081 gcctcggaga gcagcaggtc ggtcccgcgg gcgagcgcct cgacctcgtc ggtcgggccc
     1141 gtgtcgcccg tgtaggccac ggaggtgccg tcggcctgca ggcgcacgcc gatcgtcggg
     1201 acgagatgcg tcatctccca cgtccggatg cggaacggcc ccagatcggt ctcctgcccg
     1261 aatcccagct cacggcactc gaagacgctc ggcatgtcct tgccctcgtc ctcggtcagc
     1321 cggcagaagt agtcgaagaa cccgttcggg gccagcaccg ggatcggcgg gagctcctct
     1381 gggtggaaga agcgcgcaac gaacagcggg tagaggtcga ggaagtggtc gtagtgcgcg
     1441 tgcgagatga cgatgccgtc gaggtccttc aggtcgatca gctgctgcag gcgggagagc
     1501 gttccggtgc cgaggtcgag cgccacccgg aagccgtcgt ggcgcagcag gtagccgctc
     1561 gccgctcgtc cggcacccgg ccaggctccg ctggtgccca gcgcggtcag gtccacggcc
     1621 cgctccccac cccggcgacg gtcgcaccgc gcacctcgga cacctccggc ccgaggaaca
     1681 tcccaccgag cgcccggaac agctccgggt cacccgagga gacgaactcg tgccgcggcg
     1741 ggccggcggc cagggagccc gatccgacga gcgttgcgta gacgtccttc gccgtctcct
     1801 ccgcgcttga gacgagcgtc acctccggcc ccatcaccag ccgcagcagg ccggcgagga
     1861 gcgggtagtg cgtgcagccg aggacgagcg tgtccacgcc agcggccgcg agcggcgcca
     1921 ggtactgctc ggcgtgggcg cgcagcgccg gcgaggtcgt gtcgccgcgc tcgacgaacc
     1981 ggacgaatgc ggggcaggcc gcggtgagca gctccacgtc cgccccggtg cgggcgatgg
     2041 cccgctcgta cgcgccgctg gcgatggtcg cctcggtgcc gatcacgccg atccgcccgt
     2101 tccgcgtggc cgccaccgcg gcgcgcgctc ccggatcgac caccccggcc accgggaccc
     2161 cgctcgcctc ggtcagcgcc tcgatcgccg cgacctcgat cgagttgcag gccaccacga
     2221 gcatcttcac gtcgcgggag aggaggtagg cggcgatctc ccgggcgaac ccggcgatct
     2281 cctcgagcga ccgctggccg tacgggaacc gtgccgtgtc gccgaagtac agcaccgact
     2341 cgtgcggcag gaggtcgatc atggcccgga ccaccgtcag gccgccgacg ccggaatcga
     2401 acgtcccgat cggccggtcg tcgcgctcaa ggtgcccccc aagcccgtcg ctcaccacca
     2461 cagcgtctcc ccgacgttct tctccgcgcg gcccaggtcg gcggcccagg cttcggtgga
     2521 gaggtacttc cacccgccgt cggcgagcag gcagacgacg tcgccctcgt cgatctcctc
     2581 ggccaggcgg cgggcgacgt ggacgacggc gccggaggag atcccggcga agatcccctc
     2641 gcgctcgacc agctgccggg tggcgcgcag cgcgtcggag gacccgacca ggaacttccg
     2701 gtccaggatc gcctcgtcga agatcggggg gatgaacccc tcgtcgagcg accgcagccc
     2761 gtagacgagc tcgccgagct ccggctcggc agccacgatc tgcacgctcg gatcgtgctc
     2821 ccggagccgc cggccgacgc ccatcagagt cccaccggtg ccgagcccgg cgacgaaatg
     2881 cgacacatcg ggcaggtcgc ggaggatctc ggcgccggtc ccctcgtagt gcgccgccgg
     2941 gttggcgggg ttcccgtact ggaagggcat gaagtacttg tcgtcgcggg ccatcgcctc
     3001 ggcgacctcg atcgccccgt tcgttccctt ctcggccggg ctgtagacga tctcggcgcc
     3061 gaacagctcc agcaggcgga cccgctcgtc gctggcgttc tcggggatga ccaccgtcag
     3121 ccggtacccc ttcagccgcg ccacgagcgc aagggcgatg cccgtgttgc cggaggtcgg
     3181 ctccaggagc tccttccccg ggacgagcgt cccggccgcc tcggcctcct ccaccatctt
     3241 ccgggcgatg cggtccttga gggacccggt cgggttctgc ccctccagct tggcccacag
     3301 tcgaaccgag gggttcgggg acgtccgggg caggccgacg agcggcgtgt tcccgatcga
     3361 gtcgagcacg gaagcgacct tcacaggccc caattatccc aggggccggc cgaagcgacc
     3421 ggagcgccgc ccgccgctac gggcagctca ccaggacgtc gccagggtcg gcggtgcagg
     3481 tgtcgccctc ggtgccgccg tccaccgtct caccggtcac cccgtcctgg gcctgcagcg
     3541 tgtccatccc ggcgttcccg aacaggtcgt cggagtcggg gccgccggtc aggatgtcgt
     3601 cgtcggcacc gccgttcagc gtgtcgttgc ccgcgccccc gcgcaggtcg tcgtcgccgc
     3661 cgccgccgga caccgtgtcg ttccccgtcg aggcgcacac gacgtcattg cccggcgacc
     3721 cgacgatgac gtcgtcgccg cccgtcccaa ggatcgtgca gccgttcgcg ttcgcgttga
     3781 ccgtggtctg tgcggtcgcc gagtcgttgg ccggctgcgg atcggactcg ttcccggaga
     3841 ccacggcagt agcggtggcc gtgccggcca tcgacgtcga ggcgacgata tccacggtcg
     3901 ccgagcccgc caccccgatc gcaccgagcg tgcaggtaac ggtcgtcgtc ccgctgcacg
     3961 acccctggct cgccgtcgcc gactgcttgg tcgcgcccgc gggaagtgtc gtggccaccg
     4021 tgacgccggt ggccggctcg ggccccccgt tggtcacggt gaggtgatat gtgatggtgc
     4081 tgccgacggt caccgggtcg ggagagtccg acatcgtgat gccgaggttc gcgcccgtga
     4141 cgatggtctc gccctcgaac cgggccagcg cgaggtcggt gttcgcgccg gtccaggccg
     4201 agccggcgac gaccagccgg ccgtcgtcct ggatggcggc cgcccggccg atgtccgggc
     4261 ccgtcccgaa ggccagggtg gcccgcccgt cgccggagaa cgtcccgtcg aaggtgccgc
     4321 cgctctccag ccgccacagc acgaaccgcg ggtcgtcgcc cgcgggcgtg tgtcggccgg
     4381 gcacgacgag cttgccgtcg tcctggatcg cgagctcctc cgccgagtcg gtgaagctcc
     4441 cggtggcccc tgtgaccttc ccgtcgccgg agaaggacgt gtccagcgcg ccgccggtcg
     4501 tgtagcgggc gagggccacg tggtcaccgg cgaaccccgc cgcgacgatc cgcccgttcg
     4561 actggatcac cacaccgttt gcaccctcgc tcggggcgcc gaagtcggtg gtctgcacgc
     4621 catcgccgga gaacgtcgag tcggtgccgc cggaggtggt caggcggacc agcttgaaca
     4681 ccccgttccc ccaccccgcc atgacgagct tcccgtcggc ctggaccgcg aggtcgcgcc
     4741 cgaggtccag gctgtccccc gtcgtgagct gcccgttccc cgagaacatg ttgtccgggt
     4801 caccgttcga cgtgtagcgc gccgccgcga acccgcaggt gaacaggccg cacgagcgat
     4861 acccgccggc cacgatgcgg ccgtcggaca ggatcgccac cgccatggcg accacgctct
     4921 cgttgttcac gaaggtcgtg gtaagccggc cgtcgccgtt gaagttcgtg tcgaggctcc
     4981 cgttggcgtt gtaccgggcc agcgcgaacc ggttgatcgt cccgttcgag gcgcgcccgg
     5041 ccacgacgat cttgccgttc ggctggatcg ccacgccgag cgcctcctcc ccgtagacgg
     5101 agaagacggt gaccgcgcgc ccgtccccgt cgaacgtcgg gtcgagcgac ccgtccggta
     5161 gatagcgggc cacgccgaag tgccgcagcg agccgccggc gacgctcccc gccacgacga
     5221 tcttgccatc ggcctg
//
LOCUS       DASPWO010000201         8147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_200801, whole genome shotgun sequence.
ACCESSION   DASPWO010000201 DASPWO010000000
VERSION     DASPWO010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200801"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VEO00_04445"
     CDS             <1..531
                     /locus_tag="VEO00_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57283.1"
                     /translation="RAVVASLLRMPWTAKSHLRHAERWRFDQRMVAQARTTLGPGRCV
                     VLRYEDVVEDPAGARVVLAGLLRLDGGTPAGEPAAAPPEGRLIAEGEWWKEAALGPVD
                     AARVEAWREELTAAQAADVVAICRREMGWFGYDGPTATAAWARLARMSPRDRWRRARF
                     RRRLARRAAEVPPVLP"
     gene            594..1718
                     /locus_tag="VEO00_04450"
     CDS             594..1718
                     /locus_tag="VEO00_04450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU57284.1"
                     /translation="MKVLEGLARAGDVELFSFVNPGRTEPLELPAGAPVRRVEVVEAA
                     RARLTPFRRARWLLGGRLPLELATRDYRSVRSAFRRWAADRYDLVWFVRAEAFAALGG
                     AVEAPQVVDLDDLEDHKTLARLQASGPGGARGAGARMQGSVNARRWSGLQRRIAAVAE
                     RVVVCSGLDRRRLGAPNAVVIPNGYDPPAHPAGRDAIGDPPTILLQGFLLYPANVDAA
                     RFLVERVLPLVRQRVPDVGVRLVGKSDGRVERLADPPRVVVTGFVPEIAEELARADAV
                     AVPIRYGSGTRIKILEAFAHRIPVVSTALGVEGVDAVPGAHLLVADDPAEFAEACVTL
                     LTDVDRRHQLVDAAHRLFLGGYRWDTIQDRIAALARDVAG"
     gene            complement(1726..2544)
                     /locus_tag="VEO00_04455"
     CDS             complement(1726..2544)
                     /locus_tag="VEO00_04455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HYU57285.1"
                     /translation="MPLFSVVLPTHGRPAFLAEAIDSVLTQTVGDLELIVVDDASERP
                     AAVPDDARVRLVRRDTNGGPAAARNTGLDHATGRYLAFIDDDDLFVDERLAIALEGLA
                     RAPVAVCWNRYMDWPGREGRSLEGNVYDTILDGMAPHLGRTALVREVAPRFDERFRGA
                     EDVDWWLRLAKDAEVTTVPRIGYLVRRHEGARQGYGAAERARARMRLLDLHAGYYRAH
                     PRAAAFAWKRIGLLAQKAGDHRLARKAFARSFRIRPEPRTAAHLARSLRPSAGG"
     gene            2658..3809
                     /locus_tag="VEO00_04460"
     CDS             2658..3809
                     /locus_tag="VEO00_04460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU57286.1"
                     /translation="MRPARILHLIDSLGMGGAERGLAATLGHLDPGRFASEVAFLWGP
                     DLLATAIHRLGIPVHRVRAPRGPGALRAIEPVRRLLVRRRIDLIHTSVVWASIVGRIA
                     GHRAGVKVVSHVTNVDPRGFQRAELSRAVGLKARAVAWLDEWTGARYVDRFVAITEAV
                     REHPIRGGSWDRSKIVVVRRGQDLHALAAAARGDPDPPIADPGSPTILAVGRLSEQKG
                     HRVLLEAMPKVLAELPRARLSVVGDGHLRGRLQKQAEPLGDRVAFLGIRRDVPALLAR
                     CDAFCFPSLWEGQGNALLEAMAVGAPIVATSIPAVTETVRDGETAVLVPPGDPAALAD
                     GLLRVLREPGESSVMAVKARLDATRFDIRQTTRDLEAVYDDVLRPTSSA"
     gene            3812..4144
                     /locus_tag="VEO00_04465"
     CDS             3812..4144
                     /locus_tag="VEO00_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57287.1"
                     /translation="MARREGIDQVVRFGVGWTIIAVGGVVVWVIFGTEDGLSLVAGAI
                     LLAPLLGAVVASVSPWSSPALVVAAVLTGVTAVLLLIGGTGLLFIPPAVLFTVGAVRG
                     RRRRLPRA"
     gene            complement(4255..5658)
                     /locus_tag="VEO00_04470"
     CDS             complement(4255..5658)
                     /locus_tag="VEO00_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYU57288.1"
                     /translation="MSRTSDLPNVVLVVFDTARRDRFGCYGYGRPTTPTTDSLAREGL
                     VFETMISNAPWTLPAHGSLFTGLYPTEHGSQWQTGPALRDAVPLTMAEFLKGIGYDTW
                     CVTNNGLISSRTRLARGFDTYAARLDIERGLRRMARRVPKALVGGDSGGKVVNAWMRR
                     RLPRARRPMFLFVNYVECHWSYAPPPSMVRRVGGPRHRALEGLRYRTGVAAKAGPWEA
                     VARADERGLEVLSTLYDGEVADVDRHLNGLLGVLEESGHLRQPTIVIVASDHGEHLGE
                     RGLADHHASLDDLLLRVPFVVWGPGVIGGARRVPETFEFVDVLPSLVQLLDQPMPDHL
                     AGRRTGVFDGEGDGNPVSLSEWRAWSKSELRRLKRRNPSFDFTGLRRDLACARDDRFK
                     LVRGSDGSAAVYDLRADPVESADVSAAHPDAASRLAKALDAAIPRWAGWEAAGAAQIT
                     AAEEQEIEARLEELGYI"
     gene            complement(5655..5930)
                     /locus_tag="VEO00_04475"
     CDS             complement(5655..5930)
                     /locus_tag="VEO00_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYU57289.1"
                     /translation="MSAPDVRGRLRALVDEALVVTEGPPVEAAPEEDLFLPEVLDSIG
                     LVTLLGMIEDEWGVRFDDRELTLDLLESLDGLAATIASRVAAAGRKG"
     gene            complement(5927..7600)
                     /locus_tag="VEO00_04480"
     CDS             complement(5927..7600)
                     /locus_tag="VEO00_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57290.1"
                     /translation="MNRPPSEAVRAVLAEAQRREEPGLVEKTGTLSGVRGEALLFHIA
                     RPPGAARPVGLVICHSFLEFALLQGEEIAFARAAARRGFATLYVEAPGMGDSAGASED
                     CSIEDRVGAALAGFAELRRRVPEVVRPCFVGAVLGGLVAALAAARAGDASLALWHPDL
                     EPAAYWSEVRRMARVTALKQDRDVEDPQDALDRRGRAAVLGMRITRDQLADVFASAKG
                     LPAGSAGGPAFVLALQDEGGRAQVAAVRRLVRGPVDVKDVPGRRGDVWHLGLRRGGQA
                     GSWTLDWLEGVACAPPHAGDRGGRPEPARPEPMDPPSRPGEVPFFLPTSGGDLAAVAS
                     LPERGPLAGPPTLLLHSPFAPRPRGVAMLLLARRLADAGVPALRLDVPGSGLTPMADP
                     DDRVRTIEILADAARWFAAACGEKALAVAGTCLGGRYALGVAARIPWTERVTAVAAPI
                     RHASHRRRGLQGRRAPAPFQPGLPEDLRALPPTAEVTFVYGDQDAMYADFLALGDGGP
                     ADRRWEVVVRPGVRLHGFRELTIENESWVVDLLAERMAPAERASVAEPS"
     gene            complement(7597..>8147)
                     /locus_tag="VEO00_04485"
     CDS             complement(7597..>8147)
                     /locus_tag="VEO00_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57291.1"
                     /translation="PAGRPDEEAVVRMLAEAGGFFRDACGASELAIVGSCLGARLGIA
                     VAARDPAVTHVVGVAAPLGVARREPRFALRRRRPDWVPGMAEAVVAAARTARLTFVYG
                     GEDRSYRLFGEFTARAVPAEAAARLEVVAREGVRVHGFLDLSAEPERWLVDTIDAMLS
                     GETAASAASARGEAATPAGSER"
BASE COUNT         1007 a         3054 c         3050 g         1036 t
ORIGIN      
        1 cgagcggtgg tggcgtccct gctgcggatg ccctggacgg cgaagagcca cctgcgccac
       61 gccgagcgct ggcggttcga ccagcggatg gtggcgcagg cacggacgac gctcgggccg
      121 gggcggtgcg tcgtgctgcg ctacgaggac gtggtcgagg acccggccgg cgcgcgcgtg
      181 gtgctggcgg gactgctgcg actggacgga gggacccccg cgggcgagcc ggcggcggcc
      241 ccgcccgagg ggcggctgat cgcggagggc gagtggtgga aggaggccgc gctcgggccg
      301 gtcgacgctg cgcgggtcga ggcctggcgc gaggagttga cggcggccca ggcggccgat
      361 gtcgtcgcga tctgccggcg ggagatggga tggttcgggt acgacgggcc gaccgcgacc
      421 gccgcctggg cccgtctggc gcggatgtcc ccgcgcgacc ggtggcggcg agcccggttc
      481 cggcgacggc tcgcccgccg cgccgccgag gtccccccgg tccttccatg aggattctgg
      541 tcgtcgccag gagctttcca tggccgatcc gcagcggagc ggggatccgg ctggtgaagg
      601 tactggaggg gttggcacgc gccggcgacg tcgagctgtt ctcgttcgtc aaccccgggc
      661 gaaccgagcc gctcgagctt cccgccggcg cgccggtgcg ccgcgtcgag gtggtggagg
      721 ccgcgcgggc ccggctgacc ccgttccggc gcgcgcgctg gctgctcggc ggccgccttc
      781 ctctggagct cgcgacgcgc gactatcgca gcgtccgctc ggcgttccgg cgatgggccg
      841 ccgatcgcta cgacctggtc tggttcgtcc gcgcggaagc cttcgccgcg ctcggcggtg
      901 cggtcgaggc cccgcaggta gtggacctcg acgacctcga ggaccacaag acgctcgccc
      961 ggctgcaggc gtcggggccc ggcggggcgc gcggcgccgg ggcgcggatg cagggcagcg
     1021 tcaacgcccg gcggtggagc ggcctgcagc ggcgtatcgc cgccgtggcg gagcgcgtcg
     1081 tggtgtgcag cggtctcgac cggcggcggc tcggcgcgcc caacgctgtg gtcatcccga
     1141 acggctacga cccaccggcc catccggccg ggcgggacgc gatcggggac ccgccgacga
     1201 tcctgctgca ggggttcctg ctgtacccgg cgaacgtcga cgcggcgcgc ttcctggtgg
     1261 agcgggtgct gccgctcgtg cgccagcgcg ttccggacgt cggcgtgcgc ctggtcggga
     1321 agagcgatgg gcgtgtcgaa cggctggccg atcccccgcg cgtggtggtg acggggttcg
     1381 tcccggagat cgccgaggag ctcgcgcggg cggacgccgt tgcggtcccg atccgctacg
     1441 gcagcggcac gcggatcaag atcctcgagg cgttcgcgca ccgcatcccg gtggtctcga
     1501 cggcgctcgg cgtggagggc gtcgacgccg tccccggcgc gcacctgctg gtcgccgacg
     1561 acccggcgga gttcgccgag gcctgcgtca cgctgctgac cgatgtcgat cggcggcatc
     1621 agctggtcga tgccgcgcac cgcctgttcc tcggcggcta ccgctgggac accatccagg
     1681 accgcatcgc cgccctggcg cgggacgtcg ccggctgact cgcggtcaac ctcccgccga
     1741 ggggcggagg ctgcgggcca ggtgggcggc ggtgcggggc tcggggcgga tgcggaagga
     1801 ccgcgcgaac gccttccggg ccaggcggtg gtcgccggcc ttctgcgcca gcaggccgat
     1861 gcgcttccag gcgaaggcgg cggcgcgggg gtgcgcgcga tagtagccgg cgtgcaggtc
     1921 gaggagccgc atccgggccc gggcgcgctc ggcggcgccg tagccctggc gggcgccctc
     1981 gtgccggcgg accaggtagc cgatcctcgg cacggtcgtc acctcggcgt ccttcgccag
     2041 gcgcagccac cagtcgacgt cctcggcgcc tcggaagcgc tcgtcgaagc gcggcgccac
     2101 ctcgcggacc agggccgtgc gcccgaggtg cggggccatg ccgtcgagga tcgtgtcgta
     2161 cacgttgccc tcgagcgagc gcccctcgcg cccgggccag tccatgtagc ggttccaaca
     2221 caccgcgacc ggcgcccgcg ccagtccctc cagagcgatc gccagccgct cgtcgacgaa
     2281 caggtcgtcg tcgtcgatga aggcgaggta gcggccggtg gcgtggtcga ggccggtgtt
     2341 gcgcgcggcg gccggcccgc cgttcgtgtc gcgccggacc aagcgcaccc gcgcgtcgtc
     2401 cggcacggcc gccggccgct cgctggcgtc gtcaaccacg atcagctcga ggtccccgac
     2461 cgtctgggtg agcacggagt cgatcgcctc ggccaggaac gccggccggc cgtgcgtggg
     2521 gaggacgacg ctgaacagcg gcacgccgcg gagcgtagcg gagcggccgc acggggacga
     2581 tcgcgccgcg ggcgcacctt gccattcggg ggtacacgga gggcggagcc ccccgtggcg
     2641 gtgctagcgt cgccgtcgtg aggccggcgc gcatcctgca cctgatcgac agcctcggca
     2701 tgggcggcgc cgagcgcggc ctggccgcga cgctcggcca cctggatcct ggccgcttcg
     2761 cgtccgaggt ggcgttcctg tggggtcccg acctgctggc cacggccatc caccgcctcg
     2821 ggatcccggt gcaccgggtc cgggcgccgc gcgggccggg cgcgctgcgt gcgatcgagc
     2881 cggtccgccg gctgctggtc cggcgccgga tcgatctgat ccacacgagc gttgtctggg
     2941 catcgatcgt cggccggatc gccgggcatc gcgccggagt gaaggtcgtc tcgcacgtca
     3001 cgaacgtcga cccccgcggg ttccagcgtg ccgagctgtc gcgggcggtc gggttgaagg
     3061 ctcgcgccgt cgcctggctc gacgagtgga cgggggcccg ctacgtggac cgcttcgtgg
     3121 cgatcaccga ggccgtgcga gagcacccga tccggggcgg ctcgtgggac cgctcgaaga
     3181 tcgtcgtcgt ccgccgcggg caggacctcc acgcgctggc ggcggcggcg cggggcgacc
     3241 ccgacccgcc gatcgccgat cccggatcgc cgacgatcct cgcggtcggg cgcctgtccg
     3301 agcagaaggg ccaccgggtc ctgctcgagg cgatgccgaa ggtgctggcc gagctcccgc
     3361 gggcgcgcct ctccgtcgtc ggcgacggcc acctgcgcgg gcggctgcag aagcaggccg
     3421 aaccgctggg cgatcgggta gcgttcctcg ggatccgccg cgacgtcccc gcgctccttg
     3481 cccgctgcga cgcgttctgc ttcccgtcgc tctgggaggg gcaggggaac gccctgctcg
     3541 aggcgatggc ggtcggcgcg ccgatcgtcg ccacgtcgat ccccgcggtc accgagaccg
     3601 tccgcgacgg cgagacagcc gtcctcgtcc cgcccggcga cccggcggcg ctggccgacg
     3661 gcctgctgcg cgtcctgcgc gagcccggcg agtcctcggt gatggcggtc aaggcccgcc
     3721 tggacgccac ccgcttcgac atccgccaga ccacccgcga cctcgaggcc gtctacgacg
     3781 acgtgctccg gcccacctcg agcgcatgac gatggcccgg cgggagggaa tcgatcaggt
     3841 ggtccgcttc ggggtgggct ggacgatcat cgcggtcggg ggcgtggtcg tttgggtgat
     3901 ctttggaacc gaagacggtc tgagtctggt cgcgggcgcg atcctccttg cgccgcttct
     3961 cggcgctgtc gtcgcgtctg tctcgccttg gagcagccct gctctggtgg tggcggctgt
     4021 cttgacggga gttaccgcgg tcctcctgct gatcggtggg accggccttc tcttcattcc
     4081 cccggcggtt ctcttcaccg tcggagcagt tcggggacgg aggcggaggc tcccacgggc
     4141 ctgaaccaca gactgggtag cgtcaccgga ctcggatcga cggatggatc ctccgtgagg
     4201 tgggtctggc cgatcagccg gctgacggcg cacgagtcct cgagtgtgat gaacctagat
     4261 gtagccgagc tcctcgagtc tcgcttcgat ctcctgctcc tcggcggcgg tgatctgcgc
     4321 ggcgccggcc gcttcccacc cggcccagcg ggggatcgcg gcgtcgaggg ccttcgcgag
     4381 gcgcgacgcg gcgtcggggt gcgcggccga tacgtccgcc gactccacgg ggtcggcccg
     4441 caggtcgtac acggcggccg agccgtcgga cccgcgaacg agcttgaagc gatcgtcacg
     4501 ggcgcaggcc aggtcgcggc gcaggccggt gaagtcgaac gaggggttgc ggcgcttcag
     4561 gcggcgcagc tcgctcttcg accacgcgcg ccactcggac agcgacaccg ggttgccgtc
     4621 cccctcgccg tcgaacaccc cggtgcgtcg gccggcgaga tggtccggca tcggttggtc
     4681 aaggagctgt accaatgagg ggagcacgtc gacgaactcg aacgtctccg gcacccgccg
     4741 cgcgccgccg atcactcccg gcccccacac gacgaacggc acccgcagca acaggtcgtc
     4801 gagcgatgcg tggtggtcgg ccagcccccg ctcgccgagg tgctccccgt ggtccgaggc
     4861 cacgatgacg atcgtcggct ggcgcaggtg gccgctctcc tccagtaccc cgagcagtcc
     4921 gttcaggtgt cggtcgacgt cggccacctc cccgtcgtac agcgtcgaca ggacctccag
     4981 accccgctcg tcggcgcggg ccacggcctc ccacgggccg gccttcgcgg cgacgccggt
     5041 gcgatagcgc aggccctcca gcgcccggtg gcgcggcccg ccgacgcggc ggaccatcga
     5101 gggcggcggc gcgtaggacc agtggcactc cacgtagttc acgaacagga acatcggccg
     5161 ccgcgcccgc ggcagtcgcc ggcgcatcca ggcgttgacg accttcccgc ccgagtcgcc
     5221 gccgaccagc gccttcggga cccgccgggc catccggcgc agcccgcgct cgatgtccag
     5281 gcgcgcggcg taggtgtcga acccgcgggc cagcctggtc cgcgatgaga tcagcccgtt
     5341 gttcgtcacg caccaggtgt cgtagccgat ccccttcagg aactcggcca tggtcagcgg
     5401 gaccgcgtcg cgcagggccg gcccggtctg ccactggctg ccgtgctcgg tcggatagag
     5461 cccggtgaac agcgagccgt gagccggcag cgtccacggc gcgttcgaga tcatcgtctc
     5521 gaacacgagg ccctcgcgcg ccagcgagtc ggtggtcggc gtcgtcggcc tcccgtagcc
     5581 gtagcagccg aagcggtccc gccgggccgt gtcgaacacg accagtacga cgttcgggag
     5641 gtccgacgtc cggctcatcc cttcctcccc gccgcggcca cccgcgacgc gatcgtcgcg
     5701 gccaggccgt cgagcgactc caggaggtcg agcgtcagct cccgatcgtc gaagcggacg
     5761 ccccactcgt cctcgatcat gccgaggagc gtgaccagcc cgatcgagtc gagcacctcg
     5821 ggcaggaaca ggtcctcctc cggcgcggcc tccaccggcg gcccctcggt gaccacgagc
     5881 gcctcgtcga cgagcgcccg cagccgaccc cgcacgtcgg gggcgctcac gatggctccg
     5941 ccacggaggc gcgctcggcc ggggccattc gctccgccag gagatccacc acccaggact
     6001 cgttctcgat cgtcagctcc cggaacccgt gcagccgcac cccggggcga acgaccacct
     6061 cccagcgccg gtctgccggg ccgccgtctc cgagcgccag gaagtccgcg tacatcgcgt
     6121 cctggtcccc gtagacgaac gtgacctctg ccgtcggcgg gagcgcccgg aggtcctccg
     6181 gcaggccggg ctggaaggga gccggcgcgc gtcgcccctg cagcccgcgg cgacggtgac
     6241 tggcatggcg gatcggagcc gccaccgcgg tgacgcgctc ggtccacggg atccgcgccg
     6301 ccacgcccag ggcgtaccgc cccccgaggc aggtcccggc gacggccagc gccttctcgc
     6361 cgcacgcggc cgcgaaccag cgagccgcat cggcgaggat ctcgatggtc cgcacccggt
     6421 cgtccgggtc cgccatcggc gtcagccccg acccggggac gtccagccgc agcgcgggaa
     6481 caccggcatc ggccaggcgg cgcgcgagca ggagcatcgc cacgccacgc ggccgcggcg
     6541 cgaacgggga gtgcaggagc aaggtgggag ggcccgccag cgggccgcgt tccggcaggc
     6601 tggcaaccgc cgccaggtcg ccgcccgagg tcggcaggaa gaacgggacc tcgccgggcc
     6661 gcgagggagg atccatgggc tcgggccggg cgggctccgg acggcctccg cgatcgccgg
     6721 cgtgcggcgg tgcgcaggcg accccttcca gccagtcgag cgtccagctg ccggcctggc
     6781 cgccgcggcg cagcccgaga tgccagacgt ccccgcgccg tcccggaacg tccttcacgt
     6841 cgacggggcc acggaccagc cgccggacgg cggcgacctg ggctcgccca ccctcgtcct
     6901 gcagtgcgag gacgaacgca ggcccgccgg cgcttcccgc cggcagcccc ttcgccgacg
     6961 cgaacacgtc cgccagctgg tcccgggtga tccgcatgcc gagcacggcc gcccggccgc
     7021 ggcggtcgag cgcgtcctgc ggatcctcga cgtcccggtc ctgcttcagc gccgtcaccc
     7081 gggccatccg gcgcacctcg gaccagtacg ccgcaggctc gaggtcgggg tgccagagcg
     7141 cgagcgacgc gtcgcccgcc cgcgccgcgg cgagggccgc gaccagcccg ccgagcaccg
     7201 cgccgacgaa gcacggccgg acgacctcgg ggacccgccg gcgcagctcg gcgaacccgg
     7261 ccagcgccgc gcccacccgg tcctcgatcg agcagtcctc gctggccccg gcgctgtcgc
     7321 ccatccccgg cgcctccacg tagagcgtgg cgaacccgcg ccgggcggcc gcgcgggcga
     7381 aggcgatctc ctcgccctgg agcagcgcga actccaggaa cgagtggcag atcaccagcc
     7441 cgaccggtcg cgccgccccg gggggccggg cgatatggaa caggagcgcc tcgccgcgga
     7501 ccccggagag cgtgccggtc ttctcgacga gccccggttc ctcccgccgc tgcgcctcgg
     7561 cgaggaccgc gcgaaccgcc tcggacgggg gccggttcat cgctccgatc ccgccggcgt
     7621 cgccgcctcc ccccgggcac tcgcggcgga agcggcggtc tctccggaga gcatcgcgtc
     7681 gatcgtgtcc accagccagc gctcgggttc ggccgacagg tcgaggaacc cgtgcacccg
     7741 cacgccctcc cgcgccacca cctcgagccg ggcggcggcc tcggccggga cggcgcgcgc
     7801 cgtgaactcg ccgaacaggc ggtaggagcg gtcctctccc ccgtagacga acgtgaggcg
     7861 cgcggtacgg gccgcggcga cgaccgcctc ggccatgccg ggcacccagt ccggccggcg
     7921 gcggcgcagc gcgaagcgcg gctcccggcg ggccacgccg agcggggcgg cgacgccgac
     7981 cacgtgcgtc acggccgggt cgcgggcggc cacggcgatc cccagccgcg cgccgaggca
     8041 cgagcccacg atcgccagtt ccgatgcgcc gcaggcgtcg cggaagaagc cgcccgcctc
     8101 ggcgagcatg cgcaccaccg cctcctcgtc cggccgtccg gccgggg
//
LOCUS       DASPWO010000202         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_286523, whole genome shotgun sequence.
ACCESSION   DASPWO010000202 DASPWO010000000
VERSION     DASPWO010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286523"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /gene="metG"
                     /locus_tag="VEO00_04490"
     CDS             complement(<1..958)
                     /gene="metG"
                     /locus_tag="VEO00_04490"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HYU57292.1"
                     /translation="MPAKDTYYVTTPIYYVNDVPHLGHAYTTVATDVVARFHRQRGER
                     VFFLTGTDENSQKIPRAAEAAGKDPNQWLEEIVPRWREVWERLDISHDDFIRTTEERH
                     VKPVQRFAQALYDKGEVYLGTYRGLYCVSCEQFYKEDELVDGKCPVHGIPVEHVEEEN
                     YFFRLSSYQDRLLQLYEENPTFVQPDERRNEVIAFVKGGLEDLSFSRTTFTWGVPIPW
                     DPKHVIYVWVDALQNYITAVGYGDDDARFERIWPADVHFVGKDILRFHAVIWPALLMA
                     AGLPVPKQVFAHGWLLVGGEKMSKTKLTGIHPLELIDQFGTDA"
     gene            complement(1023..1880)
                     /gene="rsmI"
                     /locus_tag="VEO00_04495"
     CDS             complement(1023..1880)
                     /gene="rsmI"
                     /locus_tag="VEO00_04495"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HYU57293.1"
                     /translation="MGGSSPGTLYVVGTPIGNLGDLTERARGTLAVVGVVVAEDTRRT
                     RALLSHLGVRARLLSLPAERERERAGVVLARLRAGDDVALVTDAGMPGISDPGAHLVA
                     AALEAGIPVAAVPGPSAALAALVVSGLRADRFAFEGFLPRGGAGRAERLVALAAEPRT
                     VVLFESPRRTAATLRDLLAALGGERRAAVCRELTKLHEEVLRGTLAELVQELDQRELR
                     GEVTIVVEGGVATAAVTVEVAAGEAVALVAGGMRPRAAASEAARRHGLHANAVYAALL
                     ALRRPNGTG"
     gene            1912..>2505
                     /locus_tag="VEO00_04500"
     CDS             1912..>2505
                     /locus_tag="VEO00_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HYU57294.1"
                     /translation="MERSSRAVRFLGRPVVLIAIVTLAAGWIRFVGLSRPDILVFDET
                     YYAVDACLYLNRPLAVCNAFKEDENKLKVPSEQSWVHPPLGKYMIAAGEAIYGVRPFG
                     WRFSSALFGTLTVTLVALIAYRLTRSALWAGVAGVLLGVESLNFVQSRMSMLDIFEAF
                     WVVAGFLFLVLDRQWIDRRQTPSLEPVPLLARSAGVPA"
BASE COUNT          335 a          920 c          862 g          388 t
ORIGIN      
        1 aggcgtcggt cccgaactgg tcgatcagct cgagcgggtg aatgccggtc agcttggtct
       61 tgctcatctt ctccccgccc accagcagcc atccgtgcgc gaacacctgc ttcggcaccg
      121 gcagcccggc cgccatcagc agcgccggcc agatcaccgc gtgaaagcgc aggatgtcct
      181 tgccgacgaa gtggacgtcc gccggccaga tccgctcgaa ccgggcgtcg tcgtccccgt
      241 accccaccgc cgtgatgtag ttctgcagcg cgtccaccca gacgtagatc acgtgcttcg
      301 ggtcccaggg gatcggcacg ccccaggtga acgtggtccg gctgaacgac agatcctcca
      361 gcccgccctt gacgaacgcg atgacctcgt tccgccgctc gtcgggctgc acgaaggtgg
      421 ggttctcctc gtagagctgc agcagccggt cctggtagct cgacaggcgg aagaagtaat
      481 tctcctcctc gacgtgctcg accgggatgc cgtggaccgg gcacttcccg tcgacgagct
      541 cgtcctcctt gtagaactgc tcgcacgaca cgcagtagag gccgcgatag gtgccgaggt
      601 agacctcgcc cttgtcgtag agcgcctgtg cgaaccgctg gacgggcttc acgtggcgct
      661 cctcggtcgt gcggatgaag tcgtcgtgag agatgtccag gcgctcccag acctccctcc
      721 agcgcgggac gatctcctcg agccactggt tcgggtcctt gccggcggcc tcggccgccc
      781 gtgggatctt ctgcgagttc tcgtcggtgc cggtcaggaa gaacacgcgc tccccgcgct
      841 ggcggtggaa ccgcgccacc acgtccgtgg ccaccgtggt gtaggcgtgt cccaggtggg
      901 gcacgtcgtt cacgtagtag atcggcgtgg tgacgtagta ggtgtccttc gccggcatcg
      961 tcgggccatt gtacggatgc acacgggagg ctcccgcccc cgtgtccccc ccgcaacccg
     1021 ggtcagcccg tgccgttcgg gcggcggagg gccagcagcg cggcgtagac cgcgttggcg
     1081 tgcaggccgt ggcggcgggc ggcctccgac gcggccgcgc gggggcgcat cccgccggcg
     1141 accagggcga ccgcctcgcc ggcggcgacc tcgacggtca ccgcggcggt cgcgacgccg
     1201 ccctcgacga cgatcgtcac ctcgccgcgc agctcacgtt ggtcaagttc ctgtaccagc
     1261 tcggcgaggg tgccgcggag gacctcctca tggagcttgg tcagctcgcg gcagacggcg
     1321 gcccggcgct cgccgccgag cgcggccagc aggtcccgga gcgtcgccgc cgtccgccgg
     1381 ggcgactcga acagcacgac cgtccgcggc tcggcggcga gcgccaccag ccgctcggcc
     1441 cggccggcgc cgccacgcgg caggaacccc tcgaatgcga accggtccgc ccgcagcccg
     1501 gagaccacga gcgccgcgag cgccgccgac ggtccgggga cggcggccac ggggatgccc
     1561 gcctcgagcg ccgctgcgac caggtgggca ccggggtcgg agatgcccgg catgccggcg
     1621 tcggtgacga gcgccacgtc atccccggcc cgcagacgtg ccaggacgac ccccgcccgc
     1681 tcgcgctcgc gctccgcggg gagggacagc aggcgggcgc ggacgccgag gtgggagagc
     1741 agcgctcgtg tccgccgggt gtcctcggcc accacgacgc cgaccaccgc aagcgtcccc
     1801 cgggcgcgct cggtcaggtc gccgaggttg ccgatcgggg tgccgaccac gtacagcgtg
     1861 cccggcgacg accctcccac gcgcggatca tgccctagtg ttttcgtcgt cgtggaacgc
     1921 tcctcccgcg cggtccgatt cctcgggcgt ccggtcgtgc tcatcgccat cgtcacgctc
     1981 gccgccggct ggatccgctt cgtcggcctg tcccggccgg acatcctggt gttcgacgag
     2041 acgtactacg cggtcgacgc ctgcctctac ctgaaccggc cgctggccgt atgcaacgcc
     2101 ttcaaggagg acgagaacaa gctcaaggtg ccctccgagc agtcctgggt gcacccgccg
     2161 ctcggcaagt acatgatcgc cgccggcgag gcgatctacg gggtccgtcc gttcggctgg
     2221 cggttctcct cggcactgtt cggcacgctc acggtgacgc tcgtcgcact gatcgcctac
     2281 cggctgacgc ggagcgccct gtgggcgggc gtggccggcg tcctgctcgg cgtcgagagc
     2341 ctgaacttcg tgcagtcgcg gatgtcgatg ctcgacatct tcgaggcgtt ctgggtggtg
     2401 gccgggttcc tgttcctggt gctcgaccgg caatggatcg accgccggca gaccccgtcc
     2461 ctggagcccg tgccgcttct cgcccgttcg gccggcgtcc ccgcc
//
LOCUS       DASPWO010000203         6865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_622171, whole genome shotgun sequence.
ACCESSION   DASPWO010000203 DASPWO010000000
VERSION     DASPWO010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622171"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VEO00_04505"
     CDS             <1..651
                     /locus_tag="VEO00_04505"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYU57295.1"
                     /translation="MDNDRRAAYETLRSVDDGIRSIFTALEDRGVLDNTVVVFLSDNG
                     FLYGEHRYNGKLAVYEENVRTPLAIRYPGATAKVEPALASNVDLASTITELTGAAAAI
                     PQDGQSLVPLLTGPAPAGWYEDGLLLEKQRHTGKPNFWAVRTTDYKYVELDSNTNGVR
                     DNADEIELYDLTSDPLELVSQCTGTIGVPQCTPSYVTVQQTLSVKLAALKAAPPGG"
     gene            704..2131
                     /locus_tag="VEO00_04510"
     CDS             704..2131
                     /locus_tag="VEO00_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYU57296.1"
                     /translation="MSPLAARGRSGVLLLAVLSLLAFGSPDGPATARPSAGLAGTRPN
                     IVLILTDDQGIDSFPRTPPVMPWLQGRVQDPADRWVSFPNAFENTPVCCPSRATILSG
                     QWSHHTGVLNNGTGELFDESSTIATWLQAAGYRTGLYGRYLNDYPWDRGPYVPPGWDE
                     WHALMSGENSDTNYYGYTMNENGTPVAYGTAAEDYLTDVVRDKAVAFIEGTAPEEPFF
                     LHVTFTAPHRPWTPAPRHAQAYKTLEFPKRPNFNEADRSDKPAWVQALAGVSALKMDN
                     FLRGHARTLLAVDEAVHAMLDALAARGVLDDTVVIVLTDNGFSFGEHRLVGKGCVYEE
                     CIRTPLVIRNSAAAKRTVTNLVGNVDLAATIAGLAGVTPGRPQDGASLVPLLEGERPT
                     WRTAYLLRSDQWREVTGYWGLRTALWSYAELFSGEIELYDLTADPYQLQNLCPPPSFG
                     CGSYEAKRSRLHRMLQALKNAPPGG"
     gene            complement(2139..3032)
                     /locus_tag="VEO00_04515"
     CDS             complement(2139..3032)
                     /locus_tag="VEO00_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HYU57297.1"
                     /translation="MTFWIVVGVAAVVALLFLRAALKVVPEYQRMMVFRLGRSIGTKG
                     PGLIILIPIVDRGVRVDLREFYLEIPHQSAITQDNASISIDFIVFYKVIDAAMSVLQV
                     QNFAGAAQNIAATTLRSVVGDMSLDNVLAEREKMNEILRVKLDDVTERWGVKVTNVEV
                     REIIPPPEVQEAMTRQMSAERTRRAVVTEAEGTRQAAVTIATGNKDAAILNAEGDRQA
                     AILRAEGFSLALQRIFEIAQQVDANTMSLQYLEALKSLGASPSTKFVIPMEFASLLSG
                     VSGLTGRAFGGDGEAASRGKE"
     gene            complement(3029..4354)
                     /locus_tag="VEO00_04520"
     CDS             complement(3029..4354)
                     /locus_tag="VEO00_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HYU57298.1"
                     /translation="MNRARLVAGGVLLAGGLALAPGAANGQGQAPAVVALSVDGVVDP
                     FLASYVERGIADANAAGSAAILITIDTPGGLDSSMRRIIQSVLASRVPVICYVSPPGA
                     RAASAGTFILIACPVAAMAPGTNVGAAHPVGISGAIESQKVLNDAAAYIRSLAEQRGR
                     NADWAERAVRQSISASAEEALDLGVIDLISADRGALLADLDGESVVVAGDERVALRTA
                     GATIDDRSLGLGPRILHAVLTPDLAFLFFFLGLGLIVVELLHPGVSVPGVLGVVFLVA
                     AFIAFGLLPVTIVGLALLIASAVAFLIDLKVPGSGVATAVGVITLVLGGLLLFDRSVP
                     SARVSIPLIVTVAALLALFFAVTVRAVLLARRRPVEMGIEGVVGQTGVATTVLGPQGT
                     VYAAREHWSAESATGEPIAKGTPVRVVAMRGLRVLVEPAPVEAETREAT"
     gene            complement(4351..5712)
                     /locus_tag="VEO00_04525"
     CDS             complement(4351..5712)
                     /locus_tag="VEO00_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012410350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57299.1"
                     /translation="MAGTTERPAEEAGTSGSSADDALRNILQSAARLGVEMDEEEALR
                     WLTAMAAGQGPGSDLEVDVATGAFGHRVAMLDFSPKDLQYFRGIGRIVGFDDQPGRVE
                     TALALSGSAAQSKVQSFPGDCDFFERVNILAPTREGACRILAEIMREKALATAAGPTH
                     RFIEAKLGSYPVDVLRRGETMKAGSPIAWTLEEIREGRLTGTLPDGSAWEHSWDEAAL
                     DPGWCKLDWVVADPVRGKLSNASNMLDVTWEAPDGSITPLDGYLDSYFQEVYLDAASV
                     PIFSKLAKHMAGDALDEYVDALEKEVRKYTVEKPNFGKAAKRMYNVFRITGRYPEAAY
                     LRELFDEKMTVLYQISALVRTLDEASGADSEIPVDLLQRQIDELIMEATAVLEGETES
                     AVLQHLLAVKSALDRGEDPGERGGATDETIHAVGDVVNEFFRERVLGLPSIREYVEAL
                     AKR"
     gene            complement(5740..6411)
                     /locus_tag="VEO00_04530"
     CDS             complement(5740..6411)
                     /locus_tag="VEO00_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57300.1"
                     /translation="MDVEVTATPTGKTSFEKWLAVLVGLAAVVASLLATLQTHSSKRE
                     ELSLEIANRLSVAIFERIAGSAPVQSFSLASSQDALTVGLNGTARLIAAFDHPDVAET
                     AVAIGNASNAASDRLVRLATTMGTPPGEESGVDAHTREVAAATDDQLNALVAEQNRAV
                     DVANRFGERGNRAVFGLSLLALGAVLLGLAGVVGEGGAGRIAIVAGSAALGLSILWGA
                     SALLV"
     gene            complement(6417..>6865)
                     /locus_tag="VEO00_04535"
     CDS             complement(6417..>6865)
                     /locus_tag="VEO00_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57301.1"
                     /translation="AFRVLTAETLADRYRRQADAATGATRSYLLLEAEAQENVVRSIV
                     ESSAIAADPRYRTEDGRFDLALRLADNRAENPELVTTDPDDPQAAGDDAADEAVLLVA
                     STVPVAVAFLFGVLGQGFPNRRRAFLAAGVVFLVLGALAFMLVEVL"
BASE COUNT         1028 a         2566 c         2315 g          956 t
ORIGIN      
        1 atggacaacg accgccgcgc cgcgtatgag acgctgcgct ccgtcgacga cggcatccgg
       61 tcgatcttca cggcgctcga ggaccgcggc gtgctcgaca acacggtcgt cgtcttcctg
      121 tccgacaacg gcttcctgta cggcgagcac cgctacaacg ggaagctggc ggtgtacgag
      181 gagaacgtgc gcacgccgct cgcgatccgc tacccgggcg cgacggcgaa ggtcgagccg
      241 gctctcgcct cgaacgtcga cctggcctcc acgatcaccg agctgaccgg ggcggcggcg
      301 gccatccctc aggacgggca gagcctggtg ccactgctga ccggcccggc cccggccgga
      361 tggtacgagg acgggctgct gctcgagaag cagcgccaca ccggcaagcc gaacttctgg
      421 gccgtccgca ccaccgacta caagtacgtc gagctcgact cgaacacgaa cggcgtgcgc
      481 gacaacgccg acgagatcga gctgtacgac ctgacgagcg acccgctcga gctggtcagc
      541 cagtgcaccg ggacgatcgg cgtcccgcaa tgcacgccct cgtacgtgac ggtccagcag
      601 accctgtcgg tgaagctcgc cgccctgaag gccgccccgc ccggcggctg actcgcccgg
      661 cccctccctg ttcccgccgc cacggcggcg ctatggtgac ggcgtgtctc ccctcgcggc
      721 gcgcggccgc tcgggcgtgc tcctcctggc cgtgctgtcg ctcctggcgt tcggatcgcc
      781 cgacggtccg gcgaccgccc gaccctccgc cggcctcgcc gggacgcgcc cgaacatcgt
      841 cctgatcctc accgacgacc agggcatcga ctccttcccg cgaacgccgc cggtgatgcc
      901 ctggctgcag gggcgggtgc aggatcctgc cgaccgatgg gtgtcgttcc cgaacgcctt
      961 cgagaacacc cctgtctgct gcccgtcgcg ggccacgatc ctgtcgggcc agtggtccca
     1021 tcacaccggc gtcctgaaca acggcaccgg cgagctgttc gacgagtcgt ccacgatcgc
     1081 gacctggctg caggccgccg ggtaccgcac cggcctctac gggcggtacc tgaacgacta
     1141 cccgtgggac cggggaccgt acgtcccgcc cggctgggac gagtggcacg ccctgatgag
     1201 cggcgagaac agcgacacga actactacgg gtacacgatg aacgagaacg gcacgccggt
     1261 cgcctacggg acggcggccg aggactatct gaccgacgtg gtgcgggaca aggcggtcgc
     1321 cttcatcgag ggaacggcgc cggaggagcc gttctttctc cacgtgacct tcaccgcccc
     1381 ccaccgcccc tggacgccgg ccccccggca cgcgcaggcc tacaagacct tggagttccc
     1441 gaaacgtccc aacttcaacg aggccgatcg ttcggacaag cccgcctggg tccaggcgct
     1501 ggccggagtg tccgccctga agatggacaa cttcctgcgc ggccacgcgc gcacgctcct
     1561 cgccgtcgac gaggccgtgc acgcgatgct cgacgcgctc gccgcgcgcg gggtcctcga
     1621 cgacacggtc gtgatcgtgc tcaccgacaa cgggttctcc ttcggggagc accgcctggt
     1681 cggcaagggg tgcgtgtacg aggagtgcat ccgcaccccg ctggtgatca ggaactccgc
     1741 ggccgcgaag cggaccgtga cgaacctggt cggcaacgtc gatctcgcgg cgaccatcgc
     1801 ggggctcgcg ggggtgacgc ccgggcggcc tcaggacgga gcgagcctcg tgcccctcct
     1861 cgagggggag cgcccgacct ggcgcaccgc ctatctcctg cgctcggatc aatggcgtga
     1921 ggtcaccggg tactggggcc tgcgcaccgc gctctggtcc tacgccgagc tgttcagtgg
     1981 cgagatcgag ctctacgacc tcaccgcgga cccctatcag ctgcagaacc tgtgcccgcc
     2041 cccgtcgttc ggatgcgggt cgtacgaggc gaaacgctcg cggctccacc ggatgctgca
     2101 ggcgctgaag aacgcgcctc ccggcggctg atggccggct actccttccc cctcgaggcc
     2161 gcctcgccgt cgccgccgaa ggcccggccg gtcaggccgg agacgcccga cagcagcgac
     2221 gcgaactcca tcgggatgac gaacttcgtc gacggcgacg ccccgaggct cttcagcgcc
     2281 tcgaggtact gcaggctcat ggtgttcgcg tcgacctgct gggcgatctc gaagatccgt
     2341 tgcagcgcca gcgagaaccc ctcggcgcgc aggatggcgg cctggcggtc gccctcggcg
     2401 ttcaggatgg ccgcgtcctt gttgccggtg gcgatcgtga cggcggcctg gcgcgtgccc
     2461 tcggcctcgg tgaccacggc ccggcgggtc cgctcggcgg acatctgacg ggtcatcgcc
     2521 tcctgcacct cgggtggcgg gatgatctcg cggacctcga cgttggtcac cttgacgccc
     2581 cagcgctcgg tcacgtcgtc gagcttcacc cgcaggatct cgttcatctt ctcccgctcg
     2641 gccagcacgt tgtcgagcga catgtcgccg accaccgacc gaagcgtggt ggcggcgatg
     2701 ttctgcgccg cgccggcgaa gttctgcacc tgcagcacgc tcatcgccgc gtcgatcacc
     2761 ttgtagaaga cgatgaagtc gatcgagatc gacgcgttgt cctgggtgat cgccgactgg
     2821 tgcgggatct ccaggtagaa ctcgcgcagg tcgacccgca cgccccggtc cacgatcggg
     2881 atcaggatga tcagcccggg gcccttcgtc ccgatcgacc ggcccaagcg gaacaccatc
     2941 atccgctggt attcgggaac caccttcagc gcggcccgca ggaacagcag ggccaccacc
     3001 gccgccacgc cgaccacgat ccagaacgtc atgtcgcctc cctcgtctcg gcttccacgg
     3061 gcgcgggctc cacgagcacg cgcagccccc gcatcgcgac cacccggacc ggcgttccct
     3121 tcgcgatcgg ctccccggtg gccgactcgg ccgaccagtg ctcccgggcg gcgtacacgg
     3181 tcccctgcgg gccgaggacc gtggtcgcca cccccgtctg gccgaccacg ccctcgatcc
     3241 ccatctcgac cggccgccgc cgggccagga gcaccgcccg caccgtgacg gcgaagaaga
     3301 gcgccaggag cgcggcgacg gtgacgatca gggggatcga cactcgcgcg ctcggcacgc
     3361 tgcgatcgaa gagcagcagc ccgccgagca cgagcgtgat cacccccacc gccgtggcca
     3421 cgccgctgcc cggcaccttc aggtcgatca ggaacgcgac ggcggaggcg atcaggaggg
     3481 ccaggccgac gatcgtcacc ggcagcagcc cgaacgcgat gaaggccgcg acgaggaaga
     3541 caacgccgag caccccgggg accgagacgc ccggatggag cagctcgacc acgatcaggc
     3601 cgaggccgag gaagaagaac aggaacgcca gatcgggcgt caggacggcg tgcaggatgc
     3661 gcgggcccaa accgagcgag cggtcgtcga tcgtcgcgcc ggcggttcgg agcgccaccc
     3721 gctcgtcgcc ggcgaccacg acgctctcgc cgtcgaggtc ggccaggagc gcaccccggt
     3781 ccgccgagat caggtcgatc acgccgaggt cgagcgcctc ctccgcgctg gcgctgatcg
     3841 actggcgcac ggctcgctcg gcccagtcgg cgttgcgccc gcgctgctcg gcgaggctgc
     3901 ggatgtacgc cgcggcgtcg ttcaggacct tctgggactc gatcgcgccc gagatgccga
     3961 ccgggtgcgc cgcgccgacg ttcgtccccg gtgccatcgc cgccaccggg caggcgatca
     4021 ggatgaacgt accggcgctc gccgcccgcg cccccggcgg ggagacgtag cagatcaccg
     4081 ggacgcgcga cgcgagcacc gactggatga tccggcgcat cgaggagtcg agtcctccgg
     4141 gcgtgtcgat cgtgatcagg atcgcggcgg acccggcggc gttcgcgtcg gcgatgccgc
     4201 gctcgacgta ggaggcgaga aacgggtcca ccacgccgtc gaccgacagc gcaaccaccg
     4261 ccggcgcctg gccctgcccg ttcgccgccc cgggggcgag cgccaggcca cccgcgagga
     4321 ggacgccccc cgcgaccagc cgcgcgcggt tcatcgcttc gccagcgcct ccacgtactc
     4381 gcggatcgag gggagcccga ggacgcgctc gcggaagaac tcgttcacga cgtccccgac
     4441 ggcgtggatg gtctcgtcgg tcgcgccgcc ccgctccccg ggatcctcgc cgcggtcgag
     4501 ggccgacttc acggccagca ggtgctgcag gaccgcggac tcggtctccc cctcgagcac
     4561 ggccgtggcc tccatgatca gttcgtcgat ctgacgctgc agcaggtcca ccgggatctc
     4621 ggagtcggcc ccggacgcct cgtcgagcgt tcgcaccagc gcggagatct ggtacaggac
     4681 ggtcatcttc tcgtcgaaca gctcgcgcag gtaggcggcc tccgggtagc ggccggtgat
     4741 ccggaacacg ttgtacatgc gcttggccgc cttgccgaag ttcggcttct cgacggtgta
     4801 cttgcggacc tccttctcca gcgcgtcgac gtactcgtcg agggcgtccc cggccatgtg
     4861 cttggcgagc ttggagaaga tcggcaccga cgccgcgtcc aggtagacct cctggaagta
     4921 ggagtcgagg tagccgtcga gcggcgtgat cgacccgtcc ggcgcctccc aggtcacgtc
     4981 gagcatgttg ctcgcgttcg acagcttgcc ccggaccggg tcggcgacca cccaatcgag
     5041 cttgcaccag cccgggtcga gagcggcctc gtcccacgag tgctcccagg ccgacccgtc
     5101 ggggagcgtc ccggtcaggc gcccctcccg gatctcctcg agcgtccagg cgatcgggga
     5161 gccggccttc atggtctcgc cgcggcggag aacgtccacc gggtagctgc cgagcttcgc
     5221 ctcgatgaag cggtgcgtcg ggccggccgc ggtggccagc gccttctcgc gcatgatctc
     5281 ggcgaggatc cggcaggccc cctcgcgggt cggcgccagg atgttcacgc gctcgaagaa
     5341 gtcgcagtca cccgggaagc tctggacctt ggactgcgcc gccgaccccg agagcgccag
     5401 cgccgtctcg acccgcccgg gctggtcgtc gaacccgacg atccggccga tcccccggaa
     5461 gtactgcagg tccttcggcg agaagtcgag catggccacg cgatgaccga acgcgccggt
     5521 ggcgacgtcc acctcgaggt cgctccccgg gccctgtccg gcggccatcg ccgtcagcca
     5581 ccgcagcgcc tcttcctcgt ccatctccac gccgagccgc gcggcggact ggaggatgtt
     5641 ccgcagggcg tcgtcggcgc tcgagccgga cgtgcccgcc tcctcggcgg ggcgttccgt
     5701 ggtccctgcc atcgctggcc ccttcccggc gaggcgccgc tagacgagca gcgccgacgc
     5761 tccccatagt atcgagaggc cgagcgctgc ggaccccgcg acgatggcga tcctgccggc
     5821 gccgccctcg cccaccacgc ccgccaggcc gagcaggacc gccccgaggg ccagcagcga
     5881 cagcccgaac accgcccggt tgccgcgctc tccgaaccgg ttcgccacgt ccacggcccg
     5941 gttctgctcg gcgaccagcg cgttcagctg gtcgtcggtc gccgccgcca cctcccgggt
     6001 gtgcgcgtcg accccgctct cctcaccggg aggcgtcccc atcgtcgtgg ccaggcgcac
     6061 cagccggtcg ctggcggcgt tgctcgcgtt gccgatcgcc acggccgtct cggcgacgtc
     6121 gggatgatcg aacgcggcga tcagccgggc cgttccgttc aggcccaccg tcagcgcgtc
     6181 ctgcgaggag gccagcgaga agctctgcac cggcgcgctg ccggcgatcc gctcgaagat
     6241 cgcgaccgac aggcggttgg cgatctccag tgacagttcc tcccgtttcg acgagtgcgt
     6301 ctgcagcgtc gccagcagcg acgccaccac cgcggcgagc ccgacgagca ccgccagcca
     6361 cttctcgaac gacgtcttcc ccgtcggcgt ggccgtgacc tcgacgtcca tcgccatcac
     6421 agcacctcga cgagcatgaa cgcgagggcc ccgagaacca ggaacacgac gccggcggcg
     6481 aggaaggcgc ggcgccggtt cgggaatccc tggcccagca cgccgaacag gaacgcgacg
     6541 gccaccggaa cggtcgaggc caccagcagc acggcctcgt cagcggcgtc gtcgccggcc
     6601 gcctgcgggt cgtccgggtc ggtggtcaca agctcggggt tctcggcgcg gttgtcggcc
     6661 aggcgcagcg cgaggtcgaa ccggccgtcc tcggtccggt acctcgggtc ggccgcgatc
     6721 gccgaggact ccacgatcga gcgcacgacg ttctcctgtg cctcggcctc cagcagcagg
     6781 tacgagcgcg ttgccccggt ggcggcgtcc gcctggcgcc ggtaccggtc ggcgagcgtt
     6841 tcggccgtga gcacgcgaaa ggcct
//
LOCUS       DASPWO010000204         6814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_452492, whole genome shotgun sequence.
ACCESSION   DASPWO010000204 DASPWO010000000
VERSION     DASPWO010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452492"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VEO00_04540"
     CDS             complement(<1..860)
                     /locus_tag="VEO00_04540"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYU57302.1"
                     /translation="MTDGRTKNRMTVLAATVAFMFAALTTRLWFLQVLAQDEFAARAQ
                     DNRVRIVTVPAPRGRILDRNGKLLVTNRFSIAVTIDRTKLQADQQEQVLFRLSELLDE
                     PVADLVRRMEDLRYYTFEPVPVAFDVTKDAAIYIGEHSDDFPGVGVQPVGIREYPLNA
                     MAAHLLGYAGEISADELDEPRFAGYGQGSIVGKSGLEAVYERDLRGKEGLTKYEVNSR
                     GEVLRSLGEQKPEVGNSLVVSIDSGIQKLTEDSLELGMELARGNGFHAPGGAALVMDP
                     NNGQILAMAS"
     gene            complement(910..1416)
                     /gene="mreD"
                     /locus_tag="VEO00_04545"
     CDS             complement(910..1416)
                     /gene="mreD"
                     /locus_tag="VEO00_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HYU57303.1"
                     /translation="MRRAVSWGAVIVTVLLLQSTVFAQIKLFGAKPELTYLITVVLAM
                     LEGPASGAIAGFAAGMAQDFLLNEPKGITALTLTLLGYAVGQIRQFVVTPSPLLPVAL
                     VGGATAIGVLFNRLVAFLLGQLDVGFLFVLRTALLSAIYNAILTPLLYPVLRRVAESS
                     RAKRVFRW"
     gene            complement(1452..2276)
                     /gene="mreC"
                     /locus_tag="VEO00_04550"
     CDS             complement(1452..2276)
                     /gene="mreC"
                     /locus_tag="VEO00_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HYU57304.1"
                     /translation="MALSRRSLRSTRLLMIGLVTASLVTITVDFRGGDSGPLAAAGRA
                     ALGIISPLQNAVSRVFRPVGDFVSTITHLGSLRAEIRQLETQLAEQQQQSGSYWTLLA
                     RTHQLEELMRFKQRYGRGIGADVIAEGVSNFEWTITVNAGSDGGVKVNDPVIAPEGLA
                     GHVVEVSGSSSVVQLIIDPDSGVGARLSISRETGLLVGSREDEMRLELLDPAVDVEPG
                     EPVETSGLEGGRYPGGIPIGVVSRVLEDPSALSKEVWVRPNLDVSRLEIVVILLTH"
     gene            complement(2290..3342)
                     /locus_tag="VEO00_04555"
     CDS             complement(2290..3342)
                     /locus_tag="VEO00_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018658314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYU57305.1"
                     /translation="MQRTAPRSEGLTGFLGRDMAVDLGTANTLVYVRGRGIVLNEPSV
                     VAINTVNRAILAVGAEAKRMIGRTPSHIQAVRPLKDGVIADFDVTEKMLRYFIQKVHK
                     RRFLAKPRVVVCVPSGITGVEQRAVEEATISAGARAAYIIEEPMAAAIGAGLPIHEPT
                     GNMVVDIGGGTTEVAVVSLGGIVTANSIRVGGDELDEAIIQYVKKEYSLMLGERTSEA
                     IKLAVGSAFPTPDEMIAEIKGRDLVSGLPKTIHISAEEIRKAIEEPVNQIIDAVKNAL
                     DKCPPELAADIMDKGIVLTGGGALLKGLDERLKHETGMPIHVADNPLSCVAIGSGKCL
                     EEFEVLRRVLITSARH"
     gene            3679..4608
                     /locus_tag="VEO00_04560"
     CDS             3679..4608
                     /locus_tag="VEO00_04560"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HYU57306.1"
                     /translation="MTVAVVFPGQGSQEVGMADPWLAHPVSAAALQEASDVLETDIVE
                     VCRDGEALDRTEVVQTAVFACDLAAWRLLESAGVEPAAVAGHSLGEFVALVAAGVIEL
                     RPALVAVRDRALAMGEASASPKGGMTALVGLSPEDAAEVCQIAGRGDVLTVANENSPK
                     QTVLSGTIAAIERAEDLARTRGARPVRLKVAGAFHSPLMRPALQGVRDAVARLHFEEA
                     RFPVVPNVSGRPTTHPFALRDLLSRQLVSPVRWEKSMRAMADIGVTTIVEAGPGEVLA
                     KLAKRGVPGIAAVSVGTPGGALEFVASVREEAV"
     gene            4609..4848
                     /locus_tag="VEO00_04565"
     CDS             4609..4848
                     /locus_tag="VEO00_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYU57307.1"
                     /translation="MTPGISLAVAPVAGRVRLLPPKAFAEGREVVTKGQAVAVVEGGV
                     VRGTVVAPVAGVVDGLLVHEGEPVVTGQPVLAVRS"
     gene            4845..5846
                     /locus_tag="VEO00_04570"
     CDS             4845..5846
                     /locus_tag="VEO00_04570"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HYU57308.1"
                     /translation="MTRAARIVAAGSALPERVVPNEWFSQFVDTSDEWIRSRTGIAER
                     RFAAEGETASTLAAAAVERALASAGLEPESVDLTLVATVTGDRPFPSTAAYVQRRVGL
                     RGGAFDLAAGCAGFVYALSVGSALVSGGQAETVVIAGAEVLSRLMDLTDRGTCVLFGD
                     GAGAVVLQPSDEPGVIDSLLENDATQTELLRLPAGGSAMPTTSETIAAGENFIKMPDG
                     KEVFRRAVIGMADVCGRLLDKAGVPPEEVTLVVPHQANARIIKAVADRLKFPPERVFV
                     DVEKVGNTSAASIPVALDHAWRGGRLSPGDLVLTTAFGAGLAYGANLIRWTAESPGA"
     gene            5843..6574
                     /gene="fabG"
                     /locus_tag="VEO00_04575"
     CDS             5843..6574
                     /gene="fabG"
                     /locus_tag="VEO00_04575"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HYU57309.1"
                     /translation="MMTAFVTGGSRGIGRACAEALGAAGLAVAVGYRAGEAEAEKTAA
                     AIQEAGGRATTLALDVTDEASVDAAFDAIEGSLGAVAVLVNNAGVSVDGLALRYKAET
                     FRQTLETNLTGAFLCSRRALGPMLKARWGRIVNLSSVAGLRGSAGQSAYSASKFGLIG
                     LTRTLAREVGKRGITVNAVCPGLVETDMTDAMPEASRRAILETTPAGRMATPAEVAAV
                     VRFLAREDAGYVNGAAIPVDGGLTA"
     gene            6626..>6814
                     /gene="acpP"
                     /locus_tag="VEO00_04580"
     CDS             6626..>6814
                     /gene="acpP"
                     /locus_tag="VEO00_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYU57310.1"
                     /translation="MDRQEIEQRVRAVLAEQLAVDEAQVTMDATFADDLDADSLDLVE
                     AVMALEEALGIEIPEEEME"
BASE COUNT          961 a         2388 c         2461 g         1004 t
ORIGIN      
        1 aaggaggcca tcgccaggat ctgcccgttg ttggggtcca tgacgagcgc ggcgcccccc
       61 ggcgcgtgga acccgttgcc gcgtgccagt tccatcccca gctcgagcga gtcctcggtg
      121 agcttctgga tgccggagtc gatcgagacc accaggctgt tcccgacctc gggcttctgc
      181 tcgcccagcg agcggagcac ctcgccgcgc gagttcacct cgtacttcgt caacccttcc
      241 ttgccgcgca ggtcccgctc gtacacggcc tccagccccg acttcccgac gatcgatccc
      301 tgtccgtagc ccgcgaaacg cggttcgtcc agctcgtcgg ccgagatctc accggcgtag
      361 ccgaggagat gggcggccat cgcgttgagc ggatactccc ggatccccac cggttggacc
      421 cccacgccgg ggaagtcgtc gctgtgctcg ccgatgtaga tcgccgcgtc cttcgtcacg
      481 tcgaaggcca ccgggaccgg ctcgaacgtg tagtagcgca ggtcctccat ccgccggacc
      541 aggtccgcta ccggctcgtc gagcagctcc gagagccgga acaggacctg ctcctgctgg
      601 tcggcctgga gcttcgtccg gtcgatcgtc accgcgatcg agaaccggtt cgtcaccaag
      661 agcttgccgt tccggtcgag gatccggccg cgcggcgccg gcacggtgac gatccgcacc
      721 cggttgtcct gggcgcgggc ggcgaactcg tcctgcgcca ggacctgcag gaaccatagg
      781 cgcgtggtca gcgcggcgaa catgaacgcc acggtcgcgg cgagcacggt catgcggttc
      841 ttggttcggc cgtcggtcat gatcgtctgg cggatcgtcc ggggggagga gcgggtgatc
      901 gcagacgttc taccacctga acacccgttt cgcccgggag gactccgcca cccgccggag
      961 cacagggtag agcagcggtg tcaggatcgc gttgtagatc gccgacagca atgccgtccg
     1021 cagcacgaac aggaacccga cgtccagctg gccaagcagg aaggccacca ggcggttgaa
     1081 cagcacgccg atggcagtgg cccccccgac gagcgccacc gggagcagtg ggctcggcgt
     1141 gacgacgaac tgccggatct gtccgaccgc gtagccgagc agggtcaggg tcagcgcggt
     1201 gatccccttc ggctcgttca gcaggaagtc ctgggccatc ccggcggcga accctgcgat
     1261 cgccccggac gccgggccct cgagcatcgc cagcaccacc gtgatcagat acgtcagctc
     1321 gggcttcgcc ccgaacagct tgatctgggc gaagaccgtg gactggagca ggaggacggt
     1381 gacgatcacc gccccccaag agaccgcgcg gcgcacgggc cgagtctacc gacgccctcg
     1441 ccgtgggcgg actagtgcgt caggaggatc acgacgatct cgaggcggga gacgtcgagg
     1501 ttggggcgga cccagacctc cttcgagagg gcgctggggt cctccaggac gcgggagacc
     1561 acgccgatcg ggatgccgcc ggggtagcgg ccgccctcca ggccggaggt ctcgacgggc
     1621 tcccccggct cgacgtcgac ggccgggtcg aggagctcga ggcgcatctc gtcctcgcgg
     1681 cttccgacga gcagcccggt ttcgcggctg atcgacagac gggcgccgac gccggagtcg
     1741 gggtcgatga tcagctggac gaccgacgag gagccgctca cctcgacgac gtggccggcg
     1801 agcccctcgg gcgcgatcac cggatcgttg accttcacgc cgccgtccga cccggcattg
     1861 accgtgatcg tccactcgaa gttcgagacc ccctcggcta tcacatccgc gccgatgccg
     1921 cgtccatacc gctgcttgaa gcgcatgagc tcctcgagct ggtgggtacg cgccaggagc
     1981 gtccagtacg acccgctctg ctgctgctgt tcggcgagct gcgtctcgag ctggcggatc
     2041 tcggcgcgca gtgagccgag gtgcgtgatc gtcgagacga agtcgccgac cgggcggaac
     2101 acgcgcgaca cggcgttctg cagcgggctg atgatcccga gcgcggcgcg gccggcggcc
     2161 gcgagcgggc cgctgtcgcc cccgcggaag tcgacggtga tcgtgaccag gctcgcggtg
     2221 acgagcccga tcatcaggag ccgcgtgctg cggaggctgc gtcgggagag cgccatggcg
     2281 gcgcccggcc tagtgccggg cggaggtgat caggacgcgg cggaggacct cgaactcctc
     2341 caggcacttg ccggagccga tcgccacgca cgacagtggg ttgtcggcca cgtggatcgg
     2401 catcccggtc tcgtgcttca ggcgctcgtc gaggcccttc agcagcgccc cgcccccggt
     2461 cagcacgatg cccttgtcca tgatgtccgc ggcgagctcc ggcgggcact tgtcgagcgc
     2521 gttcttcacc gcgtcgatga tctggttgac gggctcctcg atcgccttgc ggatctcctc
     2581 cgccgagatg tggatcgtct tcggcaggcc cgacaccagg tcgcgcccct tgatctcggc
     2641 gatcatctcg tcgggcgtcg ggaacgccga cccgaccgcg agcttgatcg cctcggaggt
     2701 gcgctcgccg agcatcagcg agtactcctt cttcacgtac tggatgatcg cctcgtccag
     2761 ctcgtcgccg ccgacgcgga tcgagttcgc ggtgacgatg ccaccgagcg agaccacggc
     2821 gacctcggtc gtgccgccgc cgatgtcgac gaccatgttg cccgtcggct cgtggatcgg
     2881 gagaccggcg ccgatcgccg ccgccatggg ctcctcgatg atgtacgcgg cgcgcgcgcc
     2941 ggcggagatc gtcgcctcct cgaccgcgcg ctgctcgacg ccggtgatcc ccgagggcac
     3001 gcacaccacc acgcgcggct tcgccaggaa gcgccgcttg tgcaccttct ggatgaagta
     3061 gcgcagcatc ttctcggtca cgtcgaagtc ggcgatgacg ccgtccttca gcgggcggac
     3121 cgcctggatg tgcgagggcg tgcgcccgat catccgcttg gcctcggcgc cgaccgcgag
     3181 gatcgcccgg ttcaccgtgt tgatcgcgac gaccgagggc tcgttcagca cgattccccg
     3241 cccgcggacg tagaccaggg tgttcgccgt gccgaggtcg accgccatgt cgcggccgag
     3301 gaaacccgtc aggccctcgc tgcgaggcgc ggtgcgctgc atcgaggctt ggtgtgggaa
     3361 ctcagacatc ggccctttgt acaggagcga ccccgatctt gcaaccgcgc tgacctgcga
     3421 aaacgtggat cgaggacctc actgtcgcgt cgaatgtgaa ccatgctcca acctactgtc
     3481 ggttaggttg gcaccacatg gtaaccaatg gagcgccatt gtggtcggtt ccatcgtgac
     3541 cccgcgatcc gtgtgttcag ccacgtccgc ggtggccccc ccgcgctcac ggaaccttcc
     3601 ggtttggggg gtccgctcgc cgggcgcggt gggatcggtc gctccccagg gcgagctccc
     3661 cccacgaacc ccccggccgt gacggtcgcc gtcgtcttcc ccgggcaggg ctcccaggag
     3721 gtcggcatgg ccgacccctg gctggcacat cccgtctcgg ccgccgccct gcaggaagcc
     3781 tccgacgtgc tcgagacgga catcgtggag gtctgccgcg acggcgaggc actggatcgc
     3841 accgaggtcg tccagaccgc cgtgttcgcc tgcgatctgg ccgcctggcg gctgctcgag
     3901 tccgccggcg tcgagccggc ggcggtcgcc ggccacagcc tcggcgagtt cgtcgcgctg
     3961 gtggccgccg gcgtgatcga gctgcgtccg gcgctcgtcg cggtgcgcga ccgggccctg
     4021 gcgatgggcg aggcctccgc ctccccaaag ggcggcatga ccgccctggt cggcctctca
     4081 ccggaggacg cggccgaggt ctgccagatc gccgggcgcg gcgacgtgct cacggtggcc
     4141 aacgagaaca gcccgaagca gacggtcctc tccggcacga tcgcggcgat cgagcgggcc
     4201 gaggacctgg cgcggacccg gggggcgcgc ccggttcggc tgaaggtggc cggggcgttc
     4261 cactccccgc tgatgcgccc ggcgctgcag ggagtgcgcg acgcggtggc acggctgcac
     4321 ttcgaggagg cgcggttccc cgtggtgccg aacgtctccg ggcgcccgac gacacatccc
     4381 ttcgcgctgc gcgaccttct gtcccgtcag ctcgtatccc cggtacgctg ggagaagtcg
     4441 atgcgggcga tggccgacat cggggtgacg acgatcgtcg aggcgggccc gggcgaggtc
     4501 ctggcgaagc tcgccaagcg cggcgtgccg gggatcgcgg cggtgtcggt cggcacgccc
     4561 gggggggcgc tcgagttcgt ggccagcgtc cgcgaggagg cggtgtaggt gacgcccggg
     4621 atcagcctgg cggtcgcccc cgtcgcgggg cgcgtccgcc tgttgccacc gaaggccttc
     4681 gccgagggcc gcgaggtcgt gacgaaggga caggcggtgg ccgtcgtcga gggcggcgtg
     4741 gtgcgcggaa ccgtggtcgc cccggtggcc ggcgtggtcg acggcctcct cgtgcacgag
     4801 ggcgagccgg tcgtcaccgg ccagcccgtc ctcgcggtcc gctcgtgacc cgcgcggcgc
     4861 gcatcgtcgc ggccggctcc gcgctccccg agcgcgtggt cccgaacgag tggttctcgc
     4921 agttcgtcga cacctccgac gagtggatcc gcagccggac cggcatcgcc gagcggcgct
     4981 tcgccgccga gggggagacc gcctccacgc tcgccgcggc cgcggtcgag cgggccctcg
     5041 ccagcgccgg cctggagccc gagagcgtcg acctcaccct ggttgccacg gttaccggcg
     5101 accggccgtt cccctcaacg gccgcgtacg tgcagcgccg cgtcggcctg cgcggcggcg
     5161 cgttcgacct cgccgcgggg tgcgccggat tcgtctacgc cctgtcggtg ggctcggccc
     5221 tggtgtcggg cgggcaggcc gagaccgtgg tcatcgccgg cgccgaggtg ctctcgcggc
     5281 tgatggacct gaccgaccgc ggcacgtgcg tgctgttcgg cgacggcgcc ggggccgtgg
     5341 tcctgcagcc gtccgacgag cccggcgtga tcgactcgct cctcgagaac gacgcgacgc
     5401 agaccgagct gctgcgcctg ccggccggcg ggtcggcgat gccgaccacc tcggagacga
     5461 tcgccgccgg agagaacttc atcaagatgc cggacggcaa ggaagtgttc cggcgtgcgg
     5521 tgatcgggat ggccgacgtc tgcggccggc tgctggacaa ggccggggta ccgcccgagg
     5581 aggtcacgct cgtcgtcccg caccaggcga acgcacggat catcaaggcc gtcgccgatc
     5641 gcctgaagtt cccacccgag cgcgtcttcg tggacgtcga gaaggtcggg aacacgtcgg
     5701 cggcgtcgat ccccgtggcc ctcgaccacg cctggcgcgg cgggcgcctc tcccccggcg
     5761 acctggtgct caccaccgcc ttcggcgccg gcctggccta cggcgcgaac ctgatccgct
     5821 ggacggcgga gtccccgggc gcatgatgac ggccttcgtc accggtggat cgcgcgggat
     5881 cgggcgcgcc tgcgccgagg cgctcggcgc ggcgggcctg gcggttgcgg tgggttaccg
     5941 cgccggcgag gcggaggcgg agaagaccgc cgccgcgatc caggaggccg gcggccgcgc
     6001 cacgacgctc gccctcgacg tgaccgacga ggcgagcgtc gacgccgcgt tcgacgcgat
     6061 cgagggatcg ctcggggcgg tcgcggtgct ggtgaacaac gccggggtct ccgtggacgg
     6121 gctggccctg cgctacaagg cggagacgtt tcggcagacg ctggagacga acctgaccgg
     6181 cgcgttcctc tgctcgcggc gagcgctcgg cccgatgctg aaggcgcgat ggggacggat
     6241 cgtcaacctc tcctcggtgg ccgggttgcg cggcagcgcc ggtcagtccg cctactcggc
     6301 ctccaaattc gggctgatcg ggctgacccg caccctggcg cgcgaggttg gcaagcgcgg
     6361 gatcaccgtg aacgcggtct gccctggcct ggtggagacc gacatgaccg acgcgatgcc
     6421 cgaagcctcc cgccgggcga tcctcgagac cacgccggca ggccggatgg ccacgcccgc
     6481 cgaggtggcc gccgtggtgc gcttcctggc gcgtgaggac gccgggtacg tgaacggcgc
     6541 ggccatcccg gtcgatggcg ggctgaccgc ctaggctggg cggagcgaaa cggacgcaac
     6601 cgagggcaac gggaaaggag accggatgga tcggcaggag atcgagcaga gggtccgggc
     6661 cgtgctggcc gagcagctgg ccgtggacga ggcccaggtg acgatggacg ccacgttcgc
     6721 cgacgacctg gacgcagact ccctcgacct cgtggaggcc gtgatggccc tcgaggaggc
     6781 gctcgggatc gagatccccg aggaggagat ggag
//
LOCUS       DASPWO010000205         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_708678, whole genome shotgun sequence.
ACCESSION   DASPWO010000205 DASPWO010000000
VERSION     DASPWO010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708678"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VEO00_04585"
     CDS             <1..273
                     /locus_tag="VEO00_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HYU57311.1"
                     /translation="HVRDAATADEDLRALLEVEAVEPADGALLFTCNGRGSRLFRVSD
                     HDASLVWERLGGLPLAGFNCSGEIGPVGGKNFLHGFTASVALFVDA"
     gene            complement(569..2101)
                     /locus_tag="VEO00_04590"
     CDS             complement(569..2101)
                     /locus_tag="VEO00_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HYU57312.1"
                     /translation="MAEQPEGGIGTILAYHDSTKHSPVSIRSQAHLLDWDNKPHPLKV
                     YAGLERVPLPDDVPSLAVPALEAIGVSSVDAAAPGPDLAALARLLVVGAGVHHTVRFP
                     GGDLIHFRNYASAGALYPEEVYVACADLPGLPAGVFHFDPALRALTRLREGDHRGQLV
                     RAAASEASIARAPVVLVLTGIPWRTGWKYAERGYRHLFWDAGMILANVLALAASAKLP
                     ARVVLGFVDTDVASLLGLEERRKVPLCLVSIGLRDREVAAASGEPEEVSFEVRALSRT
                     EYVFQGILQANDAGRLHAPEEVARWREGFSGSQVGDRSGEALASPQAGLSTDSLEEVI
                     RRRGSARVFARGSIRAEVLAAILARCTVGVPTDYAPNGAHLAEPYLIANAVEGMGPGA
                     YVFRSGELWLLRAGEFRREAGFLCLEQRLGADAAATHFLMADLPRTLDALGARGYRTA
                     QLEAGIVAGRIYLGAYASRFGATGLTFYDDEVTRFFSPDAEGKSCMLVVAVGESPRLR
                     RA"
     gene            complement(2103..2495)
                     /locus_tag="VEO00_04595"
     CDS             complement(2103..2495)
                     /locus_tag="VEO00_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU57313.1"
                     /translation="MHSVDYVVLYVGDLDASLAFYRDVLGLPFKFQESGYAEFATGGT
                     KFALYERVRLPELIGRHASGGGPAGEVLLLVDDVDKEAKRLRGLGVEILAGPVDRPWG
                     HRTLHVRDPDGFVVELAQEIPRQRPRQG"
     gene            2721..2998
                     /locus_tag="VEO00_04600"
                     /pseudo
     CDS             2721..2998
                     /locus_tag="VEO00_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677021.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            3030..>3315
                     /locus_tag="VEO00_04605"
     CDS             3030..>3315
                     /locus_tag="VEO00_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010593318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYU57314.1"
                     /translation="MRGAFIYGPRDVRLEERDDPKIIEPTDAIIRLSATCVCGSDLWP
                     YRGDETVQGPAPMGHEYVGIVEEVCSAVNAIKPGQFVIGSFFASDNTCEIC"
BASE COUNT          546 a         1115 c         1161 g          493 t
ORIGIN      
        1 cacgtccggg acgcggccac cgccgacgag gatctccgcg cgcttctcga ggtcgaggcc
       61 gtcgagccgg cggatggtgc tctgctcttc acgtgcaacg ggcgaggctc gcggctgttc
      121 cgggtctccg atcacgacgc ctcgttggta tgggagagac tcgggggcct gcccctcgcc
      181 ggcttcaatt gctctgggga gatcggcccg gtcggcggga agaacttctt gcacggcttc
      241 actgcctcgg tggccctgtt cgtcgacgcc tgagcctagc caacccccga ccggatcgcc
      301 gcggcgaatc gtgccacctc acgagaccga cacaaacgtt gacgttcgtg attgtgccgg
      361 gccatgtgcg caggatctcg gaatccttgg gcagctccct tgtcgtcgga agcagcgggg
      421 gcagggaccc gtaatgttca gactaacctc gtccccgatg gagagggtac gacgatgggc
      481 tgctccttct gcgcggaagc gacagtccgg agacccgacg cagtcagcgg gacacttggg
      541 accggttcgg cccgggaagg cgcgggcctt acgcgcgacg aaggcgcggg ctctcgccga
      601 ccgcgacgac cagcatgcag ctcttcccct cggcgtccgg cgagaagaat cgggtgacct
      661 cgtcgtcgta gaacgtgagg ccggtggcgc cgaagcggct cgcgtaggcc ccgaggtaga
      721 tcctcccggc gacgatgccg gcctcgagct gcgcggtgcg gtagccgcgg gcgccgagcg
      781 catcgagcgt gcggggaaga tcggccatca ggaagtgggt ggccgccgcg tccgctccca
      841 ggcgctgctc gaggcagagg aacccggcct ctcgacggaa ttcgccggcg cggagcaacc
      901 agagctcacc gctacggaac acgtacgcgc cgggccccat cccctcgacg gcgttcgcga
      961 tcaggtaggg ctcggcgagg tgcgccccgt tcggcgcgta gtcggtcggg actccgaccg
     1021 tgcaacgtgc caggatcgcg gcgagcacct cggcgcggat ggagccccgg gcgaagacgc
     1081 gagccgaacc gcgccgccgg atgacctctt cgagggagtc ggtcgacagg cccgcctgag
     1141 gcgatgcaag cgcctcgcca gacctgtcgc ccacctgcga cccactgaag ccctcgcgcc
     1201 accgggcaac ctcctcgggg gcgtgcagcc ggccggcgtc gttcgcttgg aggatcccct
     1261 ggaacacgta ttcggtccgg gagagcgccc ggacctcgaa cgagacttcc tcgggttccc
     1321 ccgacgcggc ggccacctcc cgatccctca gcccgatcga caccaggcag agcggaacct
     1381 tccggcgttc ctcgaggccc aggagcgagg cgacgtcggt gtccacgaac cccaacacga
     1441 cccgagcggg gagcttggca gaggcggcca aagcgagcac gttcgccagg atcatcccgg
     1501 cgtcccagaa caggtggcgg tacccgcgct cggcgtactt ccacccggtg cgccagggga
     1561 tgccggtgag gacgaggacc accggggccc tggcgatgga ggcctcggag gcggccgccc
     1621 ggacgagttg gcctcggtgg tcgccctcgc ggaggcgggt aagcgcgcgg agcgccgggt
     1681 cgaagtggaa caccccggcg ggaaggccgg ggaggtccgc gcaggcaacg tagacctcct
     1741 ctgggtacag ggcgccggcc gaggcgtagt tccggaagtg gatgaggtcg ccgccgggga
     1801 acctcaccgt gtggtgcacg ccagcgccga ccaccaagag ccgcgcgagg gcggcgaggt
     1861 ccggtccggg cgcggcggca tcgaccgacg agacgccgat cgcctcgagg gccgggacgg
     1921 cgagcgacgg gacgtcgtcg gggagcggga cccgttcgag gccggcatag accttgagcg
     1981 gatgcggctt gttgtcccag tccagcaagt gcgcttggct ccgaatactg accggcgagt
     2041 gcttggtcga gtcgtggtag gcgaggatcg tgccgatgcc gccttccggt tgctcggcca
     2101 cctcagccct ggcggggccg ctggcgcggg atctcctgag ccagttcgac cacgaacccg
     2161 tcggggtcgc ggacgtggag ggtgcggtgt ccccacggcc ggtcgaccgg accggccagg
     2221 atctcgacgc ccaggccgcg cagccgcttc gcctccttat cgacgtcgtc gacgaggagc
     2281 agcacctcgc cggcaggccc gcccccggac gcatggcgtc cgatcagctc gggcagccga
     2341 acgcgctcat agagagcgaa cttcgtgcct cccgtggcga actcggcgta gccgctctcc
     2401 tggaacttaa atgggagccc gagcacgtct cggtagaacg cgagcgaagc atccagatcg
     2461 ccgacataaa ggacgacata gtcgaccgag tgcacgacgg tatctaatca cggcgtcgac
     2521 ttcggccacc ccggcggacc ctgtggggca cagaaccctc ggatcgggcc cgtgactagg
     2581 tctttcttcg agttcgctcg accttctccg cctcggcggc cacgataagg gatccagacc
     2641 gtccaccggc cgagacacct gtttcgtgaa cattgtcagg ggataaggtt gaagcgagtg
     2701 agccttcggg tgctcagagg atgctcggtg acttcgcgcc ggcgctcgtg gcttcaccga
     2761 cgacgtcctg ttcggacaag tctggccgcg cccccagctg tcgccgaagg agcgcagcct
     2821 cgtgaccgtc gcggcgttga cgacgagcgg gaacatcgac cagctcgtat tccacctcgg
     2881 ccttgccaag gagagcggcg caaccgagga ggagctcatc gaggtgatca cccacctggc
     2941 cttctacgct gggtggccga aggccatgtc cggcatggcc gtcgcgaagc gggtcttccg
     3001 cctgagacca cggctgagag gacgacacca tgcgaggagc attcatctac ggtccgcggg
     3061 acgtccgcct cgaggagcgc gacgacccga agatcatcga gccgaccgac gccatcattc
     3121 gactgtcggc cacttgtgtc tgcggttcgg acctgtggcc gtaccggggg gacgagaccg
     3181 tccaggggcc cgccccgatg ggtcacgagt acgtcggcat cgtcgaggag gtctgcagcg
     3241 ctgtcaacgc aatcaagcct ggtcagttcg tgatcggctc gttcttcgcc tcggacaaca
     3301 cctgcgagat ctgcc
//
LOCUS       DASPWO010000206         3827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1052098, whole genome shotgun sequence.
ACCESSION   DASPWO010000206 DASPWO010000000
VERSION     DASPWO010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052098"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..494
                     /locus_tag="VEO00_04610"
     CDS             72..494
                     /locus_tag="VEO00_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57315.1"
                     /translation="MESAQLQTTMAEDQVRAAILAALTPFDAVAEQNQPGMLVLETGS
                     VGKAYLAGGFRAEAKMPMRITVATAGGTAGTGVSIQVASRGGGGLMSGGILGMRKARR
                     AEKTWLDRTVAAVQPAGGMGPQQPPPPPPGPPPATPSV"
     gene            complement(486..1031)
                     /locus_tag="VEO00_04615"
     CDS             complement(486..1031)
                     /locus_tag="VEO00_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HYU57316.1"
                     /translation="MNGPRIILLGPPGSGKGTQGRRLAEALGVEHVSTGDLLRATLQT
                     GDPFGVASVMAAGGLVADAILERLLTERLPPAMVLDGYPRTAAQAAALDRLLAPDGPS
                     HVLELVVPEDVLLSRLLGRAREGDRPDDTAAIIAARFEVYRNEIEDLRRHYGARLRRV
                     DGVGSLDEVAGRLLDDVRGSD"
     gene            complement(1090..1323)
                     /locus_tag="VEO00_04620"
     CDS             complement(1090..1323)
                     /locus_tag="VEO00_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HYU57317.1"
                     /translation="MVNAYILIQTEVGKAAQVAKEVAEIKGVNAAEDVTGPYDVIVRA
                     EARNVDDLGKLVVAKIQAVDGITRTLTCPVVHL"
     gene            1410..1583
                     /locus_tag="VEO00_04625"
     CDS             1410..1583
                     /locus_tag="VEO00_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57318.1"
                     /translation="MSPKRRKRAPDDPLEPYRRVRKAVPRPGRPIPDRRDKLAERDER
                     RRRREEGEQEDGR"
     gene            1580..2212
                     /locus_tag="VEO00_04630"
     CDS             1580..2212
                     /locus_tag="VEO00_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011750934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HYU57319.1"
                     /translation="MSFADRRAAGQRLGERLLGLREEETVVLAIPRGGVEVGAEVARA
                     LGAPLDVVIPRKVRAPQNPELAIGAVAPGVRVLDERLLTILRVDPEYLDREIATEEAE
                     IARRTEAYRQGRPPVSVARKVAIVVDDGIATGSTAAAAVRWTRAQGAARTILAVPVAP
                     AEAVARLRAEVDEVVVLETPEPFYAVGQWYEEFPQLEDDEVVRLLAEATR"
     gene            2209..2511
                     /locus_tag="VEO00_04635"
     CDS             2209..2511
                     /locus_tag="VEO00_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57320.1"
                     /translation="MSVKAWLAMALAAGVTGAGIASLVRSLGNTRPGDSGRLRALIVL
                     HDVARAGFWFGLAAFFTAYALADDPDAARGILIVPLALAGVRLLTAQALARSLPPD"
     gene            complement(2520..>3827)
                     /locus_tag="VEO00_04640"
     CDS             complement(2520..>3827)
                     /locus_tag="VEO00_04640"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HYU57321.1"
                     /translation="LSKLQDDHIAEREMVRVDGYIGNVEDFRTLARLTIDRANANIAG
                     MQQKLYYPRVEGADPEVQVIYTPNLDAPGYPDDRIIAVDLQNGVTRILSSDYFGESKK
                     GGLRMWNKIVFDRGGLALHAGCKMVPVGGDQKVFLIIGLSGTGKTTTTFTTQNDSSPV
                     QDDFVALMPGGKVYATENGCFAKTFSLDPDFEPNIYRAVTQPSAYLENVYQDETGAVN
                     FSETSYTHNGRAVFEMADLGHFMDAQQLERVDYLLILNWNENIIPAVSRLSRTQAAAY
                     FMLGETTGTSAGGKAEEGRFLRIPGTNPFFPLRHEQQGNRFLELLDSHPMEVFLMNTG
                     RVGGKADDERSKKVQIPHSSAVVKAIAEGSVAWTDDPDFGYEVAGSVPGIDDPELLQP
                     RRLYERQGRSGEYADLVERFKAERAAYLRGYAGLEEEIIKAVG"
BASE COUNT          515 a         1340 c         1408 g          564 t
ORIGIN      
        1 cccggtaggg gcgccccgtg cggcggagta tcctgctcgg gccggcgtgt gacccggagt
       61 gaggaggggc gatggagagc gcgcagctgc agacgaccat ggcggaggat caggtccggg
      121 cggcgatcct ggccgcgctg acgcccttcg atgccgtcgc cgagcagaac cagcccggca
      181 tgctggtcct cgagaccgga tcggtgggga aggcgtacct ggccggcggc ttccgtgcgg
      241 aggcgaagat gccgatgcgc atcaccgtcg cgaccgccgg tgggaccgcc ggcaccggcg
      301 tctcgatcca ggtcgcctcc cggggcgggg gagggttgat gtcgggcggg atattgggga
      361 tgcgcaaggc acgccgggcg gagaagacgt ggctcgaccg taccgttgcg gccgtccagc
      421 cggccggcgg gatggggccc cagcagccac caccaccgcc gccgggtccg ccccccgcga
      481 cgccctcagt ctgagccgcg cacgtcgtcc aggagccgcc cggcgacctc gtcgagggag
      541 ccgaccccgt ccacgcgcct gagccgcgcg ccgtagtgcc ggcggaggtc ctcgatctcg
      601 ttccggtaga cctcgaaccg cgcggcgatg atcgccgccg tgtcgtccgg gcgatcaccc
      661 tcgcgggcac gccccaggag gcgggacagc aggacgtcct cgggcacgac gagctccagg
      721 acgtggctcg gaccgtcggg cgcgaggagc cggtccaggg cggcggcctg cgccgccgtt
      781 cgcggatacc catccagaac catggccggg gggaggcgct ccgtcaggag ccgttcgagg
      841 atcgcgtcgg cgaccaggcc gccggccgcc atgaccgacg ccacgccgaa ggggtccccg
      901 gtctggagcg tcgcccggag caggtcgccg gtcgagacgt gctcgacgcc gagggcctcg
      961 gccaggcggc ggccctgggt gcccttgccg ctccccggcg ggccgagaag gatgatccgc
     1021 ggcccgttca cgggttgccg gaccaggcgt taccgggctc ggcgcggcct catcccgcgc
     1081 cggcccgggc tacaggtgga cgacggggca ggtcagcgtg cgcgtgatcc cgtcgacggc
     1141 ctggatcttc gcgaccacga gcttgccgag gtcgtccacg ttgcgtgcct cggcgcggac
     1201 gatgacgtcg tacgggcccg tgacgtcctc cgcggcgttc acgcccttga tctcggccac
     1261 ttccttggct acctgcgccg ccttccccac ctcggtctgg atgaggatgt aggcgttgac
     1321 cattcgtcac ctccgcgccc gagcgaggcc acatcgtatt cagtgcggcc cgaggggaca
     1381 aacgccgccg gggcgggcat gatcgaacga tgagtcccaa gcggcgcaag cgagcgcccg
     1441 acgaccccct ggagccgtac cgcagggtgc gcaaggccgt gccgcgcccg ggccgtccta
     1501 tccccgaccg ccgcgacaag ctcgccgagc gagacgagcg gcgccgccgt cgggaagaag
     1561 gggaacagga ggatgggcga tgagcttcgc ggaccggagg gcggcggggc agcgcctggg
     1621 cgagcgcctc ctgggcctgc gcgaagagga gaccgtggtc ctggccatcc cccgcggcgg
     1681 ggtggaggtc ggggccgagg tggcgcgggc cctcggcgcg ccgctcgacg tggtcatccc
     1741 gaggaaggtc cgcgccccgc agaaccccga gctcgccatc ggcgccgtgg cgcccggcgt
     1801 gcgggtcctc gacgagcgcc tcctgacgat cttgcgggtg gacccggaat acctggaccg
     1861 cgagatcgcg acggaggagg cggagatcgc ccggcggacc gaggcgtatc ggcagggccg
     1921 tccgccggtc tcggtggcga ggaaggtggc catcgtggtg gacgacggca tcgccacggg
     1981 cagcacggcc gccgccgccg tccgctggac ccgggcgcag ggcgccgcgc ggacgatcct
     2041 cgccgtcccg gtggccccgg ccgaggccgt cgcccgcctg cgggcggagg tggacgaggt
     2101 ggtcgtcctc gagaccccgg agccgttcta cgcggtcggg cagtggtacg aggagttccc
     2161 ccagctcgag gacgatgagg tcgtgcggct gctcgcggag gcgacgcgat gagcgtgaag
     2221 gcgtggctcg cgatggccct cgcggccggg gtcacggggg cggggatcgc ctcgctggtg
     2281 cggtcgctcg gcaacacgcg ccccggggac tccgggcgcc tgcgcgcgct gatcgtgctc
     2341 cacgacgtgg cgcgggccgg gttctggttc ggcctggccg ccttcttcac cgcctacgcc
     2401 ctggcggacg accccgatgc cgcccggggc atccttatcg tgcccctggc gctggccggc
     2461 gtgcgcctgc tgaccgccca ggccctggcc cgctcgctcc ccccggactg agccgaccgt
     2521 cagccgacgg ccttgatgat ctcctcctcc aggcccgcgt agccgcggag gtacgccgca
     2581 cgctcggcct tgaagcgctc gaccaggtcg gcgtactcgc cgctccgccc ctgccgctcg
     2641 tagaggcggc gcggttgcag cagctcaggg tcgtcgatgc cggggaccga cccggcgacc
     2701 tcgtagccga agtccgggtc gtcggtccac gcgacggatc cctcggcgat cgccttgacg
     2761 accgccgacg agtgcgggat ctggaccttc ttcgagcgct cgtcgtcggc ctttccgccg
     2821 acccggccgg tgttcatcag gaacacctcc atcgggtgcg agtcgagcag ctcgaggaag
     2881 cggttgccct gctgctcgtg ccggagcggg aagaacgggt tcgtcccggg aatgcggagg
     2941 aacctgccct cctcggcctt gccgccggcg gaggtccccg tggtctcgcc gagcatgaag
     3001 tacgccgcgg cctgcgtccg cgacagccgc gacaccgccg ggatgatgtt ctcgttccag
     3061 ttcaggatca gcaggtagtc gacgcgctcc agctgctgtg cgtccatgaa atgcccgagg
     3121 tcggccatct cgaagaccgc tcgtccgttg tgcgtgtacg aggtctcgga gaagttcacg
     3181 gcccccgtct cgtcctggta cacgttctcc aggtaggcgc tcggctgcgt cacggcgcgg
     3241 tagatgttcg gctcgaagtc gggatcgagc gagaaggtct tcgcgaagca gccgttctcc
     3301 gtcgcgtaga ccttgccgcc cggcatcagc gcgacgaagt cgtcctgcac cggcgaggag
     3361 tcgttctgtg tcgtgaacgt cgtggtggtc ttcccggtcc cggagagccc gatgatcagg
     3421 aacaccttct ggtcgccacc gaccgggacc atcttgcagc cggcgtgcag cgccaggccg
     3481 ccccggtcga agacgatctt gttccacatc cgcaggccgc ccttcttcga ctcaccgaag
     3541 tagtcggagc tcaggatgcg ggtgaccccg ttctggaggt cgacggcgat gatccggtcg
     3601 tccgggtacc ccggggcatc gaggttcggg gtgtagatca cctgcacctc gggatccgca
     3661 ccctcgacgc gcgggtagta gagcttctgc tgcatcccgg cgatgttcgc gttcgcgcga
     3721 tcgatggtga gccgggcgag tgtgcggaag tcctccacgt tgccgatgta gccgtccacc
     3781 cggaccatct ctcgctccgc gatgtgatcg tcctggagct tcgacag
//
LOCUS       DASPWO010000207         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1052132, whole genome shotgun sequence.
ACCESSION   DASPWO010000207 DASPWO010000000
VERSION     DASPWO010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052132"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..436)
                     /locus_tag="VEO00_04645"
     CDS             complement(50..436)
                     /locus_tag="VEO00_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57322.1"
                     /translation="MDNVSPRGALGVTLLTALAMIGAACGSSPSGSTSPSVSGSAAGD
                     FVEVQVESGQVRGPETITVAVGDRVRFEVQADVSDEVHVHTYDLKQDVTPDEPARFDL
                     VADIPGVFEVELESAGLLLFHLEVGG"
     gene            complement(524..703)
                     /locus_tag="VEO00_04650"
     CDS             complement(524..703)
                     /locus_tag="VEO00_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57323.1"
                     /translation="MPKDLDWIFEKSLDLTAATMGGRAGAGRAPEFAAQVFKEIYATL
                     KDAAQELPAKSKPGF"
     gene            824..>2023
                     /locus_tag="VEO00_04655"
     CDS             824..>2023
                     /locus_tag="VEO00_04655"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HYU57324.1"
                     /translation="MADRTGPAPTPEPWRIKSVEPIRQISREARGHALEEAGYNVFRL
                     RSGDVFVDLLTDSGTGAMSVSQWAAIQRGDEAYAGARNWAGLEHTLRGITGYEHVIPV
                     HQGRGAETVYTRAFVDEGDFVLGNMHFDTTRAHIYNRGAWPVNLVVDEGLDPEDPSPF
                     KGDADLAKLERAVAERGVDRCPLFILTITSNTNGGQPVSLHNARAVSEFCRAHGIRLL
                     LDAARFAENAYLVREREPGMAGHSVAQIAREFFDLADGAAMSAKKDALVNIGGWLAFR
                     RREDYLEARKWAVLYEGYLTYGGMAGRDLEAMAVGLREGLDPRYLEARIRQVRLLHGM
                     LSDAGVPVMRPPGGHSVMIDVNRFLDHLPREQYPAESLAAALYLEAGVRSVGLGALAF
                     AEINPDTG"
BASE COUNT          295 a          716 c          734 g          278 t
ORIGIN      
        1 gggaccggca ggtcgaggcg cccaccgatc ccgtgcgcga ccccgctgat caaccgccga
       61 cctcgaggtg gaagagcagg agtccggccg actcgagctc cacctcgaac acccccggga
      121 tgtcggcgac gaggtcaaag cgggccggct cgtcgggggt gacgtcctgc ttcaggtcgt
      181 acgtgtgaac gtgcacctcg tcggacacat cggcctgcac ctcgaagcgc acccggtcgc
      241 ccacggcaac ggtgatcgtc tccggccctc ggacctgccc gctctccacc tggacctcca
      301 cgaaatcgcc ggcggcgctc ccgctcaccg acggcgaggt cgatccggag ggtgaggagc
      361 cgcaggccgc gccgatcatg gcgagcgccg tcaggagcgt gacgcccaac gcgccgcgag
      421 gagagacgtt gtccatcggt tcctccgttc ctcggtgggt cggcccccgc acgccggccg
      481 cgtggccatg cgaaggagga aggcccagcg ggggggatcc cggtcagaag cccggcttcg
      541 acttcgccgg cagctcctgc gccgcgtcct tcagcgtggc gtagatctcc ttgaagacct
      601 gcgccgcgaa ctcaggggcc cggccggcgc cggcccgccc gcccatcgtc gcagcggtga
      661 ggtcgagcga cttctcgaat atccagtcga ggtccttcgg catctccggt cctccctcct
      721 cgtcggggtc gaatcgtgcg gctggtaggc tagccgagcc cgcgggagag caacccgccc
      781 gcgggaacgc gggaacaggt acccctggcg cgaggaggcc gcgttggcgg atcggaccgg
      841 ccccgccccg acccccgagc cctggcgcat caagtccgtc gagccgatcc ggcagatctc
      901 gcgcgaggcg cgcgggcacg cgctcgaaga ggccgggtac aacgtcttcc gcctgcgttc
      961 gggcgacgtg ttcgtcgacc tgctgaccga ttccggcacg ggcgcgatga gcgtctcgca
     1021 gtgggcggcc atccagcgcg gcgacgaggc ctacgccggc gcgcggaact gggcaggcct
     1081 cgagcacacg ctccgcggga tcacgggcta cgagcacgtc atcccggtgc accagggccg
     1141 cggtgccgag accgtctaca cgcgagcgtt cgtggacgag ggcgacttcg tcctcgggaa
     1201 catgcacttc gacacgacgc gcgcccacat ctacaaccgc ggcgcctggc cggtgaacct
     1261 cgtggtcgac gaggggctcg accccgagga cccctcgccg ttcaagggcg acgcggacct
     1321 ggcgaagctc gagcgcgccg tcgccgagcg cggcgtcgat cggtgcccgc tgttcatcct
     1381 gacgatcacg tcgaacacca acgggggcca gccggtgtcg ctccacaacg cgcgggcagt
     1441 gtcggagttc tgccgcgcgc atgggatccg cctcctgctc gacgcggcgc gctttgccga
     1501 gaacgcctac ctggtgcggg agcgcgagcc gggtatggcg gggcactcgg tggcgcagat
     1561 cgcgcgcgag ttcttcgacc tggccgacgg cgcggcgatg agcgcgaaga aggacgcgct
     1621 ggtgaacatc ggcggttggc tggcgttccg caggcgcgag gactacctgg aggcgcggaa
     1681 gtgggccgtg ctgtacgagg ggtacctgac gtacggcggg atggccgggc gcgacctgga
     1741 ggcgatggcc gtcggcctgc gcgagggcct cgacccccgg tacctcgagg cgcggatccg
     1801 ccaggtcagg ctgctgcacg ggatgctctc ggacgccggc gtgccggtga tgcgcccgcc
     1861 cggcgggcac tcggtgatga tcgacgtcaa ccgcttcctc gaccacctgc cgcgggagca
     1921 gtacccggcg gagtcgctgg cggcggcgct gtacctcgag gccggcgtgc gcagtgtggg
     1981 gctcggcgcg ctggcgttcg ccgagatcaa ccccgacacc ggc
//
LOCUS       DASPWO010000208         3765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_962561, whole genome shotgun sequence.
ACCESSION   DASPWO010000208 DASPWO010000000
VERSION     DASPWO010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962561"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VEO00_04660"
     CDS             <1..392
                     /locus_tag="VEO00_04660"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYU57325.1"
                     /translation="GVLHVCPVGSPNTFYSDFGVPRPGGRTHQGNDIFAAYGTPIYAP
                     FDGYAYDSSNDLGGNAVKVDGAGGYVYNAHLSSFGTLGNVSAGTIIGHVGNSGNARYT
                     SPHDHFEWHPGGGAAVDPYPYLVEACR"
     gene            383..1537
                     /locus_tag="VEO00_04665"
     CDS             383..1537
                     /locus_tag="VEO00_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYU57326.1"
                     /translation="MPVRRLVAAVAVVVVALTYSGPVSADTASELKAARKRLGQLEKE
                     TAAARAELADLQARADALQVQVEAAQTALEITGHNIVLTQGAITRAEDRIAALQGRLD
                     DRARSAYIQGPGTVFDIFFGASSFADLQERLALYDRVQQQDSDLANRVRDRRETLDRK
                     KGDLRALQAQQERQKAELEEKQAEQQKQLDAQGKVVAAIEEKAAEAQTLVKTLEKQKA
                     REDAARARALAAGGGAIAGEGIFQFCPVDAPNAFGDDFGAPRYAGGYHPHAGNDIVAS
                     LGTPIRAPFDGYASDASNGLGGMSVHVDGALGYTYNAHLSRFGTLGNVSAGTIVGYVG
                     NSGDAMGGPYHDHFEWHPDTIPPNLHTSPYGYSVIGSAIDPYPYLVAACR"
     gene            1551..3524
                     /locus_tag="VEO00_04670"
     CDS             1551..3524
                     /locus_tag="VEO00_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018633474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYU57327.1"
                     /translation="MSDVSGHPWIFRTYAGYGTPRATNERFLQNLAKGQTGLSIAFDL
                     PTQTGYDADHAMARGEVGKVGVPVGHLGDMRRVFEGIPVEGMNTSMTINATAVWLLAL
                     YMSVADEHGAARRGLEGTTQNDVLKEYLSRGTYVFPPAPSMRLTTDLIAWTLAEVPRW
                     NPINICSYHLQEAGATPVLEAAYTMANAVAVLDAARARPDVAADDLPRLVGRISFFCN
                     AGIRFVEETCKLRAMARLWDRIARERYRVADETFRRFRYGVQVNSLGLTANQPENNVH
                     RILLEMLAVTLSADARARAVQLPAWNEAFGLPRAWDQQWSLRAQQILALETDLLEYGD
                     ILNGSPVIEEVTDRIETEAWDELGRVLDRGGSVEALSYMKVRLVTAHAERLRAIEAGE
                     RTVVGVNAYTEGEPSPLMHGDEEFAYQVIDPALEREQLEGLQAWRAERDAGAARAALD
                     RLKAVAEGGENLVPPSIDAAKAGATTGEWADALREVFGEFRAPTGVTDVTAAETDALL
                     AARARVMAAAGRLGVARLRMLVGKPGLDGHSNAAEQIAVRARDCGAEVVYQGIRLTPE
                     EIARAAAEEDVHVIGLSVLSGAHNLLVPDVIDRLKAEGIDLERVPLVVGGVIPDDDAA
                     KLASLGVARVFTPKEHDLTAMVAEIADLVAARA"
     gene            complement(3546..>3765)
                     /locus_tag="VEO00_04675"
     CDS             complement(3546..>3765)
                     /locus_tag="VEO00_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57328.1"
                     /translation="IVFVCDAYHAMTTDRPYRASLRIGEAIRRLKLSAGTQFDPRVVE
                     VFLRLHARGKIDFHGHGGAAHPAHAATS"
BASE COUNT          568 a         1323 c         1393 g          481 t
ORIGIN      
        1 gcggcgtcct gcacgtctgc ccggtcggga gtcccaacac cttctacagc gacttcggcg
       61 tcccccggcc gggcgggcgc actcaccagg ggaacgacat cttcgccgcc tacgggacgc
      121 cgatctacgc tccgttcgac ggctacgcat acgacagctc gaacgacctc ggcgggaacg
      181 ccgtgaaggt ggacggcgcc ggcggctacg tctacaacgc gcacctgtcg agcttcggga
      241 cgctcggcaa cgtctcggcc ggaacgatca tcggccacgt cgggaactcc gggaacgccc
      301 gctacaccag cccgcacgac cacttcgagt ggcacccggg cggcggcgcg gccgtcgacc
      361 cgtaccccta cctggtcgag gcgtgccggt gaggcggctg gtggcggccg tcgccgtggt
      421 ggtggtcgcg ctgacgtact ccgggccggt cagcgccgac accgcctccg agctgaaggc
      481 cgcccgcaag cgcctggggc agctggaaaa ggagactgcg gccgcccgcg ccgagctggc
      541 cgacctgcag gcgcgcgccg acgcgctgca ggtccaggtg gaggcggccc agaccgcgct
      601 cgagatcacg gggcacaaca tcgtgctgac ccagggtgcg atcacgcgcg ccgaggaccg
      661 catcgccgcg ctccaggggc ggctcgacga ccgggcgcgc agcgcgtaca tccaggggcc
      721 cggcaccgtc ttcgacatct tcttcggcgc cagctcgttc gccgacctcc aggagcggct
      781 ggcgctctac gatcgcgtcc agcagcagga ctcggacctg gccaaccgcg tgcgcgatcg
      841 ccgggagacg ctcgaccgga agaagggcga cctgcgcgcg ctgcaggccc agcaggagcg
      901 gcagaaggcc gagctggagg agaagcaggc cgagcagcag aagcagctcg acgcccaggg
      961 gaaggtcgtg gcggcgatcg aggagaaggc ggccgaggcg caaacgctcg tcaagacgct
     1021 cgagaagcag aaggcgcgcg aggacgccgc gcgggcccgc gcactggcgg cgggcggcgg
     1081 cgcgatcgcg ggggagggga tcttccagtt ctgcccggtg gacgcgccga acgcgttcgg
     1141 cgacgacttc ggcgccccga gatacgccgg cggctaccac ccgcacgccg ggaacgacat
     1201 cgtcgcgtcg ctcggcacgc cgatccgggc ccccttcgac ggctacgcct ccgatgcgtc
     1261 gaacgggctc ggcggcatgt cggtccatgt cgacggcgcg ctcgggtaca cgtacaacgc
     1321 ccacctgtcg cgcttcggga cgctcggcaa cgtcagtgcc gggacgatcg tcggctacgt
     1381 cgggaactcc ggtgacgcga tgggtggccc gtaccacgac cacttcgagt ggcacccgga
     1441 cacgatcccg ccgaacctgc acaccagccc gtacggctac agcgtgatcg gctccgcgat
     1501 cgacccgtac ccgtacctgg tcgcggcctg ccgatagccg cggcggtacg ctgtcggacg
     1561 tgagcggtca cccctggatc ttccgtacct acgccggcta cgggacgccg cgagcgacga
     1621 acgagcggtt cctgcagaac ctggcgaagg ggcagaccgg gctctcgatc gcgttcgacc
     1681 tgccgaccca gaccggctac gacgccgacc acgcgatggc ccgtggcgag gtcgggaagg
     1741 tcggcgtccc ggtcgggcac ctcggcgaca tgcgccgggt gttcgagggg atcccggtcg
     1801 aggggatgaa cacctcgatg acgatcaacg ccacggccgt ctggctgctg gcgctgtaca
     1861 tgagcgtcgc cgacgagcac ggcgcggcgc ggcgcggcct ggagggcacg acccagaacg
     1921 acgtgctgaa ggagtacctc tcacgcggga cctacgtgtt cccgccggcc ccctcgatgc
     1981 gcctgaccac cgacctgatc gcctggaccc tggccgaggt gccgcgctgg aacccgatca
     2041 acatctgctc ctaccacctg caggaggccg gggcgacgcc ggtgctcgag gccgcgtaca
     2101 cgatggccaa cgccgtcgcg gtgctcgacg cggcccgggc ccggcccgac gtcgcagcgg
     2161 acgacctgcc ccggctcgtc gggcggatct cgttcttctg caacgccggg atccgcttcg
     2221 tcgaagagac gtgcaagctg cgggcgatgg cgcggctgtg ggaccgcatc gctcgggagc
     2281 gataccgcgt ggcggacgag acgttccgcc ggttccgcta cggcgtgcag gtgaactcgc
     2341 tcgggctgac cgcgaaccag ccggagaaca acgtccaccg gatcctgctc gagatgctgg
     2401 cggtgacgct ctccgccgac gcccgggcgc gcgcggtgca gctcccggcc tggaacgagg
     2461 cgttcggcct gccgcgcgcc tgggaccagc agtggtcgct tcgggcgcag cagatcctgg
     2521 cgctcgagac cgacctgctc gagtacggcg acatcctgaa cggctcgccc gtgatcgagg
     2581 aggtcaccga ccgcatcgag accgaggcct gggatgagct cgggcgcgtg ctcgaccgcg
     2641 gcggctcggt cgaggcgctg tcgtacatga aggtgcgtct ggtgacggcg cacgcggagc
     2701 gcctgcgcgc catcgaggcc ggcgagcgca ccgtggtcgg cgtgaacgcc tacaccgagg
     2761 gcgagccctc gccgctcatg cacggcgatg aggagttcgc ctaccaggtg atcgaccccg
     2821 cgctcgagcg cgagcagctc gaggggctgc aggcctggcg cgcggaacgg gacgcgggcg
     2881 cggcccgcgc cgcgctcgac cggctgaagg ccgtcgcgga gggcggggag aacctcgttc
     2941 cgccctcgat cgacgcggcg aaggccggcg cgaccaccgg cgagtgggcc gacgcgttgc
     3001 gcgaggtgtt cggcgagttc cgcgcgccaa ccggcgtgac cgatgtcacg gccgctgaga
     3061 ccgacgcgct tctcgccgcg cgagcgcggg tgatggcggc ggcgggacgg ctcggggtgg
     3121 cccgcctgcg gatgctcgtc ggcaagccgg ggttggacgg ccactcgaac gcggccgagc
     3181 agatcgcggt gcgcgcccgg gattgcggcg cggaggtggt ctaccagggc atccggctga
     3241 cgcccgagga gatcgcccgc gcggcggcgg aggaggatgt ccacgtcatc gggctctcgg
     3301 tgctctccgg cgcgcacaac ctgctggtcc ccgacgtcat cgaccgtctg aaggccgagg
     3361 ggatcgacct ggagcgcgtc ccgctggtcg tcgggggcgt gatccccgac gacgacgcgg
     3421 cgaagctcgc ctccctcggc gtcgcccggg tgttcacccc gaaggagcac gacctcacgg
     3481 cgatggtcgc cgagatcgcc gatctggtgg ccgcccgcgc ctgaccggca gggaggccgg
     3541 cccgattacg aggtggccgc gtgcgcgggg tgcgcggcgc cgccgtggcc gtggaagtcg
     3601 atcttccccc gggcgtgcag tcggaggaac acctcgacga cccgggggtc gaactgggtg
     3661 ccggcggaga gcttcaggcg ccggatcgcc tcgccgatcc gcagcgacgc gcggtacggg
     3721 cggtcggtgg tcatcgcgtg gtacgcgtcg cagacgaaca cgatg
//
LOCUS       DASPWO010000209         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_287459, whole genome shotgun sequence.
ACCESSION   DASPWO010000209 DASPWO010000000
VERSION     DASPWO010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287459"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..453)
                     /locus_tag="VEO00_04680"
     CDS             complement(28..453)
                     /locus_tag="VEO00_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57329.1"
                     /translation="MRLLRRSLYAYALVSAVGGAALAAFPRSVLVGLFGQVHYPEYAW
                     VRVVGVQALGTAMLAVLVAQRVRDQWWWTWAFVIPVMLLFLVFGVNAVLGPGCASLPG
                     GERECPSSALWWVATAISAALAGGMVVGLARAGREAPPL"
     gene            533..1918
                     /locus_tag="VEO00_04685"
     CDS             533..1918
                     /locus_tag="VEO00_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYU57330.1"
                     /translation="MANDARVAVIGLDCGTPQLLFDRLQDELPNISNLMARSMWGELE
                     SITPPITIPAWACAMSGTTPGRLGIYGMRNRKDTTYDGLSIATSGAVTQPQLWDLLGA
                     AGKKSLLIGVPPGYPPKPVEGWRISCFLTPPSAKSYTWPEDLAAEVEAEVGEYIFDIP
                     NFREKGERFVLDQVYAMTERRFKVARRFIREKPWDYFMLVEMGPDRLHHVFWHYCDPA
                     HPKYVPGNEFETAFRDYYRYLDREVGSLLEAIPDDAVTILMSDHGARPMIGGICFNDW
                     LMREGYLAMTSSLDGATPIGKAPIDWTKTTAWGDGGYYGRLFVNLKGREPDGVVDPAD
                     YEKVRDDLIAKLEAMPGPEGRPLGTIVYRPQDVYPEVRGVAPDLIVYFGDLEWRSVGT
                     VGTGDVYTFENDTGPDGANHAQHGVFMMTGAPGQPAGRQEGLRLIDIGPTLMKLYGLE
                     EPEGVQGRSML"
     gene            1924..2502
                     /gene="cysC"
                     /locus_tag="VEO00_04690"
     CDS             1924..2502
                     /gene="cysC"
                     /locus_tag="VEO00_04690"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012362314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HYU57331.1"
                     /translation="MTTGVATQERTLGPRDRGPDRGFTVWFTGLSGAGKSTLAHLLVE
                     ELRGRDFKIELLDGDIVRTNLSKGLGFSKEDRDTNILRIGFVANLLTRNGVAAVVSAI
                     SPYRDVRDKVRALIGDFVEVYVNAPIEVCESRDVKGLYAKARAGEITGFTGVDDPYEP
                     PAQPEVEVRTDLHSEDECLAQILAALEERGYL"
     gene            2548..>2871
                     /locus_tag="VEO00_04695"
     CDS             2548..>2871
                     /locus_tag="VEO00_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57332.1"
                     /translation="MNDTATPSRAPGLVLLLGAILVIVSGFLAWFGLTFAGGSDDLKG
                     FDLTMGVGAVVISAVVLILAILVIVGVGRRALSITAIVLSAILLIIGAYAAFDPEGAI
                     TSFEAG"
BASE COUNT          456 a         1004 c         1008 g          403 t
ORIGIN      
        1 gttccgccgg cgggggccgg acggtagcta gaggggcgga gcctctcgcc cggcgcgggc
       61 caggccgacc accatcccgc cggccagcgc cgcgctgatc gcggtggcca cccaccagag
      121 ggcgctgctc gggcactctc gttcgcctcc cggcagcgac gcgcacccgg gaccgaggac
      181 cgcgttcacc ccgaacacca ggaagagcag catcaccggg atgacgaacg cccaggtcca
      241 ccaccactgg tcgcggacgc gctgagccac cagcacggcg agcatggccg tccccagggc
      301 ctgcaccccg accacgcgga cccaggcgta ctcggggtaa tggacctggc cgaacaggcc
      361 gaccaggacc gaccgcggga aggccgccag ggcggccccg ccgaccgccg agaccagcgc
      421 gtacgcgtac agggaccgcc gcaggagccg cacgacttgg taggataccg gggctctggg
      481 cggccgcggc cgtccccttc cgacccgggt cgacgatccc agaaggaacc tcgtggcgaa
      541 cgacgcgcgc gtggcggtga tcggactgga ctgcggcacc ccgcagctcc tgttcgatcg
      601 cttgcaggac gagctgccga acatctccaa cctgatggcc cgctccatgt ggggcgagct
      661 ggagagcatc accccgccga tcacgatccc ggcgtgggcc tgcgcgatga gcgggacgac
      721 ccccgggcgg ctcggcatct acgggatgcg caaccgcaag gacaccacct acgacggcct
      781 ctcgatcgcc acgagcgggg cggtcaccca gcctcagctg tgggacctgc tcggggcggc
      841 cggcaagaag tcgctgctga tcggcgtgcc gcccggctac ccgccgaagc cggtcgaggg
      901 ctggcggatc tcctgcttcc tgacgccccc ctccgcgaag tcctacacct ggccggagga
      961 ccttgccgcc gaggtcgagg ccgaggtcgg cgagtacatc ttcgacatcc cgaacttccg
     1021 cgagaagggc gagcgcttcg tgctcgatca ggtctacgcg atgaccgagc gccgcttcaa
     1081 ggtggcccgg cgcttcatcc gcgagaagcc ctgggactac ttcatgctcg tcgagatggg
     1141 gccggaccgc ctgcaccacg tgttctggca ctattgcgac ccggcgcacc cgaagtacgt
     1201 tcccggcaac gagttcgaga ccgcgttccg ggactactac cgctacctcg accgcgaggt
     1261 cggctcgctc ctggaggcga tccccgacga cgccgtgacg atcctgatgt ccgaccacgg
     1321 ggcgcggccg atgatcggcg ggatctgctt caacgactgg ctgatgcgcg agggctatct
     1381 ggcgatgacc tcgtcgctcg acggcgcgac gccgatcggc aaggcaccga tcgactggac
     1441 caaaacgacg gcctggggcg acggcgggta ctacgggcgc ctgttcgtca acctgaaggg
     1501 ccgcgagccc gacggcgtgg tcgatcccgc cgactacgag aaggtgcgcg acgatctgat
     1561 cgcgaagctc gaggcgatgc ccgggccgga gggccggccg ctcggcacga tcgtgtaccg
     1621 gccgcaggac gtctaccccg aggtgcgcgg ggtggccccc gacctgatcg tgtacttcgg
     1681 cgacctggag tggcgctcgg tcggcacggt cggcaccggc gacgtgtaca cgttcgagaa
     1741 cgacaccggc cccgatggcg ccaaccacgc gcagcacggg gtgttcatga tgaccggcgc
     1801 gccgggccag ccggccggcc ggcaggaggg cctgcgcctg atcgacatcg gcccgacgct
     1861 gatgaagctc tacgggctgg aggagcccga gggcgtccag ggaaggagca tgctgtgacc
     1921 gagatgacga ccggggtggc gacgcaggag cggacgctcg gcccgaggga ccggggcccc
     1981 gaccggggct tcacggtctg gttcacgggc ctgtccgggg ccggcaagtc cacgctcgcc
     2041 cacctgctgg tcgaggagct ccgcgggcgg gacttcaaga tcgagctcct cgacggcgac
     2101 atcgtgcgca cgaacctgtc caagggcctg gggttctcca aggaggaccg cgacacgaac
     2161 atcctgcgga tcgggttcgt ggccaacctg ctgacccgca acggcgtcgc cgcggtcgtg
     2221 tcggccatct ccccctaccg cgacgtgcgc gacaaggtgc gcgcgctgat cggtgacttc
     2281 gtcgaggtct acgtcaacgc gccgatcgag gtctgcgaga gccgcgacgt gaagggcctc
     2341 tacgccaagg cccgcgccgg cgagatcacg gggttcaccg gcgtggacga tccctacgag
     2401 cccccggcgc aacccgaggt cgaggtccgt accgacctgc actccgagga cgagtgcctt
     2461 gcccagatcc tcgccgcgct cgaggagcgc ggctacctgt agcggtccta cccgcgtaga
     2521 ggtcgccggc cgaaggagga ggggatcatg aacgacaccg caacgccatc gagggcaccc
     2581 ggcctcgtgt tgctcctggg ggcgatcctc gtgatcgtct ccggcttcct ggcgtggttc
     2641 gggctgacgt tcgccggagg gtccgacgac ctgaaggggt tcgacctcac catgggggtc
     2701 ggcgcggtgg tgatctcggc cgtcgtgctg atcctggcta ttctggtgat cgtcggtgtc
     2761 gggcggcgcg ccctgtcgat cacggccatc gtgctgtcgg cgatcctgct gatcatcggt
     2821 gcctacgccg ccttcgaccc cgagggcgcg atcacctcgt tcgaggcggg g
//
LOCUS       DASPWO010000210         3594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1052426, whole genome shotgun sequence.
ACCESSION   DASPWO010000210 DASPWO010000000
VERSION     DASPWO010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052426"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VEO00_04700"
     CDS             complement(<1..1076)
                     /locus_tag="VEO00_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017945808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYU57333.1"
                     /translation="MEVLDTFLDAMGNTPLVRLSTITRGVKPIILAKCEMLNPGGSVK
                     DRIGLRMIVTAEREGHLPAGGTIVEPTSGNTGHGLAIAAAIRGYRCIFVMPDKMSAEK
                     IALLRAYGAEVVVCPTAVPPESPESYYRVADRLTEEIPGAFQPNQYFNQENPAAHYET
                     TGPEIWRQTDGRIDVLVAGIGTGGTITGTGRYLKEQKPSVRVVGADPEGSVFSGDEPR
                     PYLVEGIGEDFWPETFDPSVVDEYVKVSDRDSLLTARAVTREEGILVGGSGGTAVFAA
                     LEVAKDLGEEHVVVVILPDTGRNYLSKLYSDTWMLDHGFVERREVIRIGEVLKAKHGQ
                     TPPLVTIGAHAKVRQAIGVLQEFD"
     gene            complement(1124..2215)
                     /locus_tag="VEO00_04705"
     CDS             complement(1124..2215)
                     /locus_tag="VEO00_04705"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYU57334.1"
                     /translation="MRRILGTLIALALTAPLGSAAPVHAASTAVGAQLVVGGLAFPAA
                     FTFAKDGTIYYGERYTGKIRSYRPSTGQFKDVWTIKNVVTNGEQGLLGLALHPDFPTN
                     RYLFAYATRNVSGTPKNQIIRITVQNGVGSASRRIFTSQTNAGTYHDGGRILFGPDRR
                     LYAVVGESHMPANAQNLGNNAGKILRMTGGGLRPSDNPFPNSLIWSYGHRNSFGFTFD
                     PQTGLIWETEAGPSCNDEINLVEKGKNYGWGPSQTCAGTAPGNTNKDGPSPVLPLAWF
                     TPTVTPTGIAFCLSTGCGLTGAQGTAFYDEYETGVIYQMTLTGDRRGIASRVSVHDHP
                     ATVLSMERGPDGALYFSSTDGGIYKLVQT"
     gene            2353..>3594
                     /gene="hutU"
                     /locus_tag="VEO00_04710"
     CDS             2353..>3594
                     /gene="hutU"
                     /locus_tag="VEO00_04710"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HYU57335.1"
                     /translation="MSARTVCAPRGRELSCRGWPQEAALRMLMNNLDPEVAERPEDLI
                     VYGGTGKAARSWEAFDAIVRALRALEGDETLLVQSGKAVGVFRTHPEAPRVLISNAML
                     VPKWADWETFWELERAGLTMYGQMTAGSWIYIGTQGILQGTYETLAELGQQHFGGSLA
                     GRVVLTAGLGGMGGAQPLAVTMNEGIALCVEVDPARIERRIKTRYLDRETDDLDEALR
                     WVERARAGGEAVSIGLSGNAAETHPELLRRGFAADVVTDQTSAHDPLAGYVPAGLTVE
                     EAAGLRVSDPEGYVKRAHASMAAQVEAMVGFQERGAVVFDYGNNLRAGAEQGGAASAY
                     AYPGFVQAFIRPLFCEGKGPFRWAVLSGDPADIAATDRAILALFPDDERLHRWIRMAE
                     ERVAFQGLPARICWLGYGERDR"
BASE COUNT          486 a         1239 c         1301 g          568 t
ORIGIN      
        1 atgtcgaact cctgcagcac gccgatcgcc tggcgcacct tggcgtgcgc gccgatcgtc
       61 accagcggcg gggtctgccc gtgcttcgct ttcaggacct ccccgatccg gatgacctcg
      121 cgccgctcga cgaagccgtg atcgagcatc caggtgtccg agtagagctt ggacaggtag
      181 ttccgcccgg tgtcggggag gatcacgacg accacgtgct cctcgccgag gtccttcgcc
      241 acctccagcg cggcgaacac cgccgtcccg ccggagccgc cgaccaggat cccctcctcg
      301 cgggtcacgg cgcgggccgt cagcagcgag tcacggtcgg ataccttgac gtactcgtcg
      361 acgacggacg ggtcgaacgt ctcgggccag aagtcctcgc cgatcccctc caccaggtac
      421 gggcggggct cgtcgccgga gaagaccgat ccctccgggt cggcgccgac cacgcggacc
      481 gacggcttct gctccttcag gtagcgcccg gtgccggtga tcgtcccgcc ggtcccgatc
      541 cctgcgacca gcacgtcgat ccgcccgtcg gtctggcgcc agatctcggg gccggtggtc
      601 tcgtagtgcg cggccgggtt ctcctggttg aagtactggt tcggctggaa cgcccccgga
      661 atctcctcgg tcagccggtc ggcgacgcgg tagtaggact ccggggactc gggcgggacg
      721 gcggtgggac agacgaccac ctcggcgccg tacgcgcgca gcagcgcgat cttctcggcg
      781 gacatcttgt ccggcatgac gaagatgcag cgatacccgc ggatcgccgc ggcgatcgcc
      841 aggccgtgcc ccgtgttgcc cgaggtcggc tcgacgatcg tgccgccggc cggcaggtgg
      901 ccctcccgct cggccgtcac gatcatccgc aggccgatgc ggtccttgac ggacccgccc
      961 gggttgagca tctcgcactt cgcgaggatg atcggcttca ccccgcgcgt gatcgtgctg
     1021 aggcgcacga ggggcgtgtt gcccatcgcg tccaggaacg tgtcgaggac ttccatcccg
     1081 ggctcccttg ccttccttcc cgtcgagggc caggacagtt tccctaggtc tgcaccagtt
     1141 tgtaaatgcc cccgtcggtg gacgagaagt agagggcgcc gtccgggccc cgttccatcg
     1201 agagcaccgt tgccgggtgg tcgtggacgc tcacgcgcga ggcgatgccg cgccggtcgc
     1261 cggtgagcgt catctggtag atcacgccgg tctcgtactc gtcgtagaac gcggtgccct
     1321 gcgcgcccgt caggccgcag ccggtcgaga gacagaacgc gatcccggtc ggggtgaccg
     1381 tcggggtgaa ccaggccagc ggcaggaccg ggctcggacc gtccttgttc gtgttccccg
     1441 gcgccgtccc ggcgcacgtc tggctcggtc cccagccgta gttcttgccc ttctcgacca
     1501 ggttgatctc gtcgttgcag ctcggcccgg cctcggtctc ccaaatgagc ccggtctgcg
     1561 ggtcgaaggt gaacccgaac gagttgcggt gcccgtacga ccagatcagc gagttcggga
     1621 acgggttgtc ggacgggcgc aggcccccgc cggtcatccg caggatcttc ccggcgttgt
     1681 tgccgagatt ctgggcgttc gccggcatgt gcgactcgcc gaccaccgcg tacagccgac
     1741 ggtcgggccc gaacaggatc cgcccgccgt cgtggtaggt cccggcgttc gtctggctgg
     1801 tgaagatcct ccgcgacgcg gagccgaccc cgttctggac ggtgatccgg atgatctggt
     1861 tcttcggggt gccgctcacg ttccgggtgg cgtaggcgaa caggtacctg ttggtgggga
     1921 agtcggggtg cagcgccagc ccgagcaggc cctgctcgcc gttcgtgacc acgttcttga
     1981 tggtccagac atccttgaac tgcccggtgg agggcctgta cgagcggatc ttccccgtgt
     2041 aacgctcgcc gtagtagatc gtcccgtcct tggcgaacgt gaacgccgcc gggaacgcca
     2101 gcccgcccac caccagctgc gcgcccaccg cggtcgaggc ggcgtggacc ggggcggcgc
     2161 tcccgagcgg agcggtcagc gcgagcgcga tcagggttcc caggatccgt cgcatgcctg
     2221 ttcccttcct ccgtccggtt cggtccgctc cattctgtcg gcttcccact ccggccgctt
     2281 gaccgaccac acggccatac gggcgggcgg ggcgtcggga cgcaccggcc gcgctaggct
     2341 ccggggagag cgatgagcgc caggacggtg tgcgcgccgc gggggcggga gctctcgtgc
     2401 cgggggtggc cgcaggaggc cgccctgcgg atgctgatga acaacctcga ccccgaggtg
     2461 gccgagcgcc ccgaggacct gatcgtctac gggggcaccg gcaaggccgc gcggtcctgg
     2521 gaggcgttcg acgccatcgt ccgcgcgctg cgtgccctgg agggcgacga gaccctgctg
     2581 gtgcagtccg gcaaggcggt cggggtgttc cggacccacc ccgaggcccc gcgcgtgctc
     2641 atctcgaacg cgatgctggt gccgaaatgg gccgactggg agacgttctg ggagctcgag
     2701 cgcgccggcc tgacaatgta cggccagatg acggccgggt cgtggatcta catcgggacc
     2761 caggggatct tgcaggggac ctacgagacg ctcgccgagc tgggccagca gcatttcggc
     2821 gggtcactgg ccggtcgggt ggtgctcact gccggcctcg gcgggatggg gggcgcgcag
     2881 ccgctcgcgg tgacgatgaa cgaggggatc gcgctgtgcg tcgaggtgga cccggcgcgg
     2941 atcgagcgcc ggatcaagac gcgctacctg gaccgcgaga ccgacgacct cgacgaggcg
     3001 ctgcgctggg tcgagcgggc ccgcgccggc ggcgaggctg tgtcgatcgg cctctccggg
     3061 aacgcggccg agacgcaccc cgagctgctg cgccgcgggt tcgccgcgga cgtggtcacc
     3121 gaccagacca gcgcgcacga cccgctggcc gggtacgtgc cggccggcct gaccgtggag
     3181 gaggccgccg ggctgcgcgt ctccgacccc gaggggtacg tgaagcgggc ccacgcctcg
     3241 atggccgccc aggtcgaggc gatggtcggc ttccaggagc gcggcgcggt ggtgttcgac
     3301 tacggcaaca acctgcgcgc cggcgccgag caggggggcg ccgcgagcgc gtacgcctat
     3361 cccgggttcg tgcaagcgtt catccgcccg ctgttctgcg aggggaaggg cccgtttcgc
     3421 tgggcggtgc tgtcaggaga cccggcggac atcgcggcca ccgaccgggc gatcctcgcg
     3481 ctgttcccgg acgacgagcg gttgcaccgc tggatccgga tggccgagga gcgggtggcg
     3541 ttccaggggc tccccgcccg gatctgctgg ctcgggtacg gcgagcgcga ccgc
//
LOCUS       DASPWO010000211         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_31462, whole genome shotgun sequence.
ACCESSION   DASPWO010000211 DASPWO010000000
VERSION     DASPWO010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31462"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VEO00_04715"
     CDS             complement(<1..336)
                     /locus_tag="VEO00_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57336.1"
                     /translation="MKGGITSGVVYPWAICAIAKRYRLHSIGGTSAGAIAAAAAAAAE
                     VGRRAAGGGFARLAELPEWLGAPPVSARGTNLRNLFRPSAATAPLFRIAARAFGDRSS
                     GGAVPVALRA"
     gene            complement(458..1372)
                     /locus_tag="VEO00_04720"
     CDS             complement(458..1372)
                     /locus_tag="VEO00_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57337.1"
                     /translation="MTELLVGTDRGLFRAGDGEAATVPEAPGGRIAALAEDGGTVWVL
                     ADGSSLVRSEDGAWRDAAILTPWRGTCLLPDGDAVLVGTAEAGLLEVRGGATSPVTGF
                     DAVPGRGDWYTPWGGPPDTRSLARGPEGALHANVHVGGIPRSDDGGATWRPTIDVDAD
                     VHQVLAHPARPDVVLASTARGLAVGTDGGRSWTFHTEGLHGPYSRAVALAGDGETVLV
                     SASEGHVGRRSALYRGTLGDAIRFARCAEGLPESFDANIDTATLVAAGERAAFGTADG
                     RVYVSEDVGATWALTASGLPPVRALHLG"
     gene            complement(1369..>2600)
                     /locus_tag="VEO00_04725"
     CDS             complement(1369..>2600)
                     /locus_tag="VEO00_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYU57338.1"
                     /translation="TRAMGTPGYDASVEHVADRLAEAGLEPHTERFTMPAFFQDGPTD
                     LAVGDRRFEDGRQVRALLYSPPGNVAAPLAPLGLDAGGPGRGGGCLAGDWGAFPAGSV
                     ALLQPGPCFRRQQVLEAQDTGAIAVIFWFAAPAGSLLRPTLIDPAGLRVPVVGVSQDA
                     GGALASSAGRTASLRTRTRTDVIEARSVLAEVAGTDPERVIVLGGHLDSVIDGPGVND
                     NGSGVALLLALADALSGTKPEATIRFEFWGGEELGLWGSNAYVDLLGAERDRIGAYLN
                     LDMVGSPNFARLVYDDRDAAEGSEAITRAFLDHFAALGLPADTIDISGRADHGAFQRA
                     GIPTGGLFSEAEEVKRAQEAARYGGTAGEPYDACYHQACDRLQRVDVDLVVEMAAATL
                     AVTADLAGLERPASMAA"
BASE COUNT          338 a         1005 c          957 g          300 t
ORIGIN      
        1 cgcccggagc gccacgggga ccgcgccgcc cgagctccgg tccccgaagg cccgggcggc
       61 gatgcggaac agcggcgccg tcgccgcgct cggccggaac aggttgcgga ggttcgtgcc
      121 gcgggccgag acggggggag cgcccagcca ctcgggaagc tctgccaggc gggcgaaccc
      181 accgcccgcc gcccggcgac cgacctcggc cgcggccgcg gcagccgcgg cgatcgcgcc
      241 ggcgctggtg cccccgatgc tgtgcagccg gtatcgcttc gcgatagcgc agatcgccca
      301 cggatacacg actccgctcg tgatgccgcc cttcatcacg acgtcgcagt cgagcgtggg
      361 gctcgcgtag acggccggat cgaggaggtc tccgggcagc ggtgcgcgca cggacgtgtc
      421 catcgccgct cctttgtcgc cggtggtagg tgtcgggtca gcctaggtgc aacgcgcgca
      481 cggggggaag cccggacgcg gtcagcgccc acgtcgcgcc cacgtcctcg gagacgtaca
      541 cgcgcccgtc ggcggtcccg aaagcggcgc gttccccggc ggcgacgagc gtcgcggtgt
      601 cgatgttcgc gtcgaaagac tccgggaggc cctccgcgca gcgcgcgaag cggatcgcgt
      661 caccgagcgt gccgcggtac agcgccgatc ggcgcccgac gtgaccctcg gaagccgaga
      721 cgagaacggt ctcaccgtcg cccgcgagcg ccaccgcccg cgagtagggg ccgtgcaggc
      781 cctccgtgtg gaaggtccac gaccggccgc cgtcggtgcc gaccgcgagc cctcgggcgg
      841 tggaggccag gaccacgtcc ggccgggccg ggtgggccag cacctggtgc acgtcggcgt
      901 cgacgtcgat cgtcgggcgc cacgtcgcgc cgccgtcgtc ggaacggggg atgccgccga
      961 cgtggacgtt cgcgtgcagc gcgccctccg ggccacgggc gagcgaacgg gtgtcgggcg
     1021 gcccgcccca gggcgtgtac cagtcgcccc gtcccgggac ggcgtcgaac ccggtgaccg
     1081 ggctcgtcgc gccgccgcgc acctccagga gcccggcctc ggccgtcccg acgaggacgg
     1141 cgtcgccgtc cggcagcagg caggtgccgc gccacggcgt caggatcgcg gcgtcgcgcc
     1201 acgcgccgtc ctccgaacgg accagactcg agccgtcggc cagcacccag acggtgccgc
     1261 cgtcctcggc gagcgccgcg atcctcccgc ccggcgcctc gggcaccgtg gctgcctctc
     1321 cgtcgccggc ccggaacagc ccgcggtcgg tccccaccag cagttcggtc atgccgccat
     1381 gctagcgggc cgctccaacc cggccaggtc cgcggtcaca gccagcgtgg ccgcggccat
     1441 ctcgacgacc aggtcgacgt cgacccgctg caggcggtcg caagcctggt ggtagcaggc
     1501 atcgtagggc tcgccggcgg tgccgccgta gcgcgcggcc tcctgcgccc tcttgacctc
     1561 ctcggcctcg ctgaacagcc cgccggtggg gatgccagcg cgctggaacg cgccgtggtc
     1621 ggcccggccg cttatgtcga tcgtgtcggc cggcagcccg agcgcggcga agtggtcgag
     1681 gaaggcgcgc gtgatcgcct ccgagccctc cgccgcgtcc cggtcgtcgt acacgagccg
     1741 cgcgaagttc ggggagccga ccatgtcgag gttgaggtat gccccgatcc ggtcgcgctc
     1801 ggcgccgagc aagtccacgt aggcgttcga cccccacagg cccagctcct cgccgcccca
     1861 gaactcgaac cggatcgtgg cctccggctt ggtcccggac aacgcgtcag cgagggccag
     1921 gagcagggcg acgcccgacc cgttgtcgtt gacccccgga ccgtcgatca ccgagtccag
     1981 gtggccgccg agcacgatca cgcgctcggg atcggtcccg gcgacctcgg cgagcacgct
     2041 gcgcgcctcg atcacgtccg tccgcgtccg ggtgcgcagc gaggcggtcc gcccggcgga
     2101 tgacgccagg gcccctccag cgtcctgcga gacgccgacg acggggacgc gcagcccggc
     2161 cggatcgatg agcgtggggc gcaggaggga cccggcgggc gcggcgaacc agaagatcac
     2221 cgcgatggct ccggtgtcct gcgcctcgag gacctgctgc cgccggaagc acggccccgg
     2281 ctggagcagc gccaccgacc cggccgggaa cgccccccag tcgcccgcca ggcagccgcc
     2341 gccccggccc ggcccgccgg cgtccaatcc gagcggcgcg agcggcgcgg cgacgttccc
     2401 gggcggggag tacaggagcg cccgcacctg ccgcccgtcc tcgaatcggc ggtcgccgac
     2461 ggcgagatcg gtcgggccat cctggaagaa cgccggcatc gtgaaccgct ccgtgtgtgg
     2521 ctcgagcccg gcctccgcca gccggtcggc gacatgctcg accgacgcgt cgtagccggg
     2581 cgtccccatg gcccgcgtgc
//
LOCUS       DASPWO010000212         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_623616, whole genome shotgun sequence.
ACCESSION   DASPWO010000212 DASPWO010000000
VERSION     DASPWO010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623616"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VEO00_04730"
     CDS             <1..543
                     /locus_tag="VEO00_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYU57339.1"
                     /translation="PRNRLVAGALHGRSIATLLFDLLTPSEERDRANVFDIALLAGRL
                     VRATAWLRERPEATGLPVGYFGASTGAGAALAAAADLGHAIAAVVSRGGRPDLAGPRL
                     PEVTAPTLLVVGGNDEVVLGLNREAEARLRCPTRLEVVPGATHLFEEPGTLERVAALA
                     AEWFAEHFAPNGRNGTTESG"
     gene            588..866
                     /locus_tag="VEO00_04735"
     CDS             588..866
                     /locus_tag="VEO00_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018530347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HYU57340.1"
                     /translation="MATKRQTQAAKRNIKKAISAARRQRTITKLPAAVRRDLGRQASR
                     ARQRGGRAGHALEDRTRTQLYEEAKRMGIEGRSKMGKWDLIRAIRKAG"
     gene            1102..2220
                     /locus_tag="VEO00_04740"
     CDS             1102..2220
                     /locus_tag="VEO00_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HYU57341.1"
                     /translation="MSELLEVLDAVRSFRDAGEALALATIVDVRGSTYRRPGARLVVT
                     RRGDLVGNLSGGCLEGEVADVARAVMEDGRPRLASFDLTADDEAVWGWGLGCNGAFEV
                     FVEPAEPAAAVGEAIRSAMEAERPLAIVTVIESADDGVPAGARVLVGTEGSAEGSLGN
                     RDADGGAASAAREALAAGRSEIRALPSPAGGMRAFVEVVEPPLRLLVCGAGHDAIPLV
                     RFAASLGWRVDVVDDRDAFLASERFPEARAFVYVERPEEAARASGADARTYVVVMTHN
                     YLRDRGYLRAFLESDVAYLGMLGPRRRLERLLGDLRDEGVLAAGEPPDRLHGPAGLDV
                     GAEGPEEIAWAIVGEVLAVRRGRAGGFLRDRSSPIHQR"
     gene            2217..>2789
                     /locus_tag="VEO00_04745"
     CDS             2217..>2789
                     /locus_tag="VEO00_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYU57342.1"
                     /translation="MNVTGAPGHVAVVILAAGTGSRMGRVKQMLPLAGRPVLQHVLDA
                     AAASGAGEIVVVLGHAADEVTAALALHPHARTVMAADHREGQSASLRTGLRALGPGAR
                     AAVILLGDQPTLPPEAIRSVIETFERTGGPVVQARYGGKPGHPVALGRAVWEAVEALR
                     GDAGARDLLAEHPEWIVHADVEGAPPPDLDT"
BASE COUNT          370 a          960 c         1097 g          362 t
ORIGIN      
        1 ccgcgcaacc ggctggtggc cggggcgctg cacggacgct cgatcgccac cctgctgttc
       61 gacctcctga cgccgtcgga ggaacgcgac cgcgccaacg tcttcgacat cgcgctgctc
      121 gccgggcggc tggtgcgcgc caccgcctgg ctgcgggagc gcccggaggc gaccggactg
      181 ccggtcggat atttcggggc gagcaccggc gccggcgctg cgctggcggc cgccgccgac
      241 ctcggccacg cgatcgcggc ggtcgtgtcg cgcggcggac ggccggatct cgccgggccg
      301 cgactccccg aggtcacagc gcccaccctg ctcgtggtcg gcggcaacga cgaggtcgtg
      361 ctcggcctga accgggaggc ggaggcgcgg ctgcgctgcc cgacccggtt ggaggtggtt
      421 cccggcgcaa cccatctgtt cgaggagccc gggacgctgg agcgcgtggc cgcgctggcc
      481 gccgagtggt tcgcggagca cttcgccccg aacgggcgga atggaaccac cgaaagcggg
      541 taggcacaga tcgaaccggg tgtccttcga tctggaggtg aagggcaatg gcgacgaagc
      601 ggcagacaca agccgccaag cgcaacatca agaaggcgat cagcgcagcg cggcgtcagc
      661 gcacgattac caagctgccc gcggccgtgc ggcgggacct cggtcgccag gcttcgcggg
      721 cccggcaacg aggtggcagg gcggggcacg ccctcgagga tcgcacgcga acgcagctct
      781 acgaggaagc gaagcgcatg ggcatcgagg gccgctcgaa gatggggaag tgggacctga
      841 tccgagcgat ccgcaaggct ggctgagggg cgattcagcg gcggctacct tcgatcctcg
      901 aggatccacg cggcggtgag gatgagggcg aaaccgccga ccgccaccag cgcgatggcc
      961 ccagaccggc gcgccaacca cgtagaggag cacggccagc gcctcgagga ccaccgcggc
     1021 cgcatacacg acacgacgat cgtacgccgc cgtccgcgtg cccgccgccg cccgttcgtc
     1081 tggcattagg ctgcatgtgc cgtgagcgaa ctgctcgagg tcctcgatgc cgtgcggtcg
     1141 ttccgcgacg ccggcgaggc gctggcgctg gcgacgatcg tcgacgtccg gggctccacg
     1201 taccggcggc ccggcgcgcg gctggtggtc acccggcgag gcgacctggt cggaaatctg
     1261 agcggtggct gtctggaggg cgaggtcgcc gacgtcgctc gtgcggtgat ggaggacggc
     1321 cggccccgcc tggcttcgtt cgacctcacg gccgacgacg aggccgtctg gggctggggt
     1381 ctcggctgca acggtgcctt cgaggtcttc gtcgagcccg ccgagcccgc ggccgcggtg
     1441 ggcgaggcga tccgctcggc gatggaggcg gaacgaccgc tcgcgatcgt caccgtgatc
     1501 gagtcggccg acgacggcgt gccggccggg gcgcgtgtgc tggtgggcac ggagggatcg
     1561 gccgaggggt cgctcgggaa cagggacgcc gacggcggcg cggcatcggc ggcgcgggag
     1621 gcgctcgccg ccggtcgttc cgagatccgg gccctgccgt cgccggccgg cggcatgcgg
     1681 gccttcgtcg aggtggtcga gccgccgctc cgcctcctgg tgtgcggcgc ggggcacgac
     1741 gcgatccccc tggttcggtt cgccgcctcg ctcggctggc gcgtcgacgt cgtggatgac
     1801 cgcgatgcgt tcctggcgag cgagcgcttc ccggaggcgc gcgcgttcgt gtacgtcgag
     1861 cgaccggagg aggcggcacg ggcctccggc gcggacgcgc ggacgtacgt ggtcgtgatg
     1921 acccacaact acctgcgcga tcggggctac ctgcgggcgt tcctggaatc cgacgtggct
     1981 tacctcggca tgctcggccc gcgccgccgc ctggagcggc tgctgggtga cctgcgcgac
     2041 gagggggtgc tggcagccgg tgagcctccc gatcgactgc acggcccggc cggtctcgac
     2101 gtgggagccg agggtccgga ggagatcgcc tgggccatcg tgggggaagt gctggcggtt
     2161 cgccgtggcc gcgccggagg gttcctgcgc gatcgatcga gcccgatcca ccagcggtga
     2221 acgtcacggg cgcgcccggt cacgtggcgg tcgtgatcct cgcggcgggc acggggtcgc
     2281 gcatgggccg cgtcaagcag atgcttccgc tcgccgggcg gccggtactg cagcacgtcc
     2341 tggacgcggc cgccgcgtcg ggagcgggcg agatcgtcgt cgtgctcggc cacgccgccg
     2401 acgaggtgac cgccgcgctc gcgctgcacc cccacgcgcg aaccgtcatg gccgccgatc
     2461 accgtgaggg gcagtccgcg tcgctccgga ccggcctgcg agcgctgggg cccggcgcga
     2521 gggcggcggt gatcctgctc ggggatcagc cgacgcttcc ccccgaggct atacgcagcg
     2581 tgatcgagac attcgagcga accggcgggc cggtggtcca ggcccgctat ggggggaagc
     2641 cgggacaccc cgtggcgctg ggccgcgcgg tgtgggaggc ggtggaggcc ctgcgcggcg
     2701 acgcaggcgc gcgcgacctc ctcgccgaac acccggagtg gatcgtgcac gccgacgtgg
     2761 agggggcgcc gccgccggac ctcgacacc
//
LOCUS       DASPWO010000213         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_799703, whole genome shotgun sequence.
ACCESSION   DASPWO010000213 DASPWO010000000
VERSION     DASPWO010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799703"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VEO00_04750"
     CDS             complement(<1..836)
                     /locus_tag="VEO00_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU57343.1"
                     /translation="MDFALTEEQEAFRRTVRRFADEVVAPRAEEMDAAEEFPLDIVKQ
                     MGELGLFGLPFPERYGGQGADFLSFCLAIEELARVDSSVAITLEAAVGLGANPIYRFG
                     TEAQKERWLVPMCRAEILGAFGLTEPDGGSDAGATRTTATLEGGEWVINGTKAFITNA
                     GTPITGLVTITAVTGTEPGGHKEISSIIVPTGTEGFSAGKAYRKIGWHASDTRELVFH
                     DCSVPEENLLGERGEGFRNFLQVLDDGRIAIAALAVGLAQGALDQSVAYARERRAFGH
                     PIG"
     gene            complement(865..>2967)
                     /locus_tag="VEO00_04755"
     CDS             complement(865..>2967)
                     /locus_tag="VEO00_04755"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYU57344.1"
                     /translation="RPQQDPGAAHRTLVEGAFPQQEIQHGFKTLLRGMSGRGPVALIL
                     EDAHAAKPAMLDLVDHVVRRIGAAPLFVLTVAREDLLEHRREWGSGLDAVTLRLDPLD
                     PEQSAELALASGGEAIDLDTARAVAEQTGGNPFFIVETTGMLMHASREGEWRRAPLPM
                     TVQAVVASRLDHLNQPARDLARKMSVFRPPVHRTELALVAAVTEQAIRELEEEEILVP
                     DDRVKDAWRFRHQFLRDVAYESLPKRERVRLHAAAADRLDQPDLKRRFPEALAYHLEQ
                     AARNALDLDPTDRTPADRAKHALGEAGDRLRRRMDFRGSIDLYERALALAGPNERWGE
                     DEAGILAGIGESQYWLAEYSIARANLERALEIAPDSLRVQAHASRFLGDIVLNVDGDV
                     DGAEALFDRARAAADAMDDPWVTARVKLMAAWVYSWRGDPQAAEREWREALEIAHRNG
                     DRWAEARALGSLCSTVSSTGDEREAQRLAEQALRLGEEGEDRFSSAVALQHIGNSYRR
                     TGRYAEAIEPLDRAIVLFDELGARWEWAASIGDRGHVRLVTGDLDAAERDLKKAQGVM
                     EALEEKGYLGFIHEALALVAAQRGDASRAERHLGLADRATSPDEFGGEASLLEARGEV
                     LWLLGDRQGARESFGKALEFYTGTPYVNAWARLATDLARRYGEDTLDSADLKRARKTM
                     ERSGWALPPLPVSVTETG"
BASE COUNT          380 a         1108 c         1060 g          419 t
ORIGIN      
        1 ccgatcgggt ggccgaacgc gcgccgttcc cgcgcgtagg ccacgctctg gtcgagcgcg
       61 ccctgggcga gcccgaccgc gagcgccgcg atggcgatgc gcccgtcgtc gagcacctgc
      121 aggaagttgc ggaacccctc cccgcgctcc ccgagcaggt tctcctccgg cacggagcag
      181 tcgtggaaca ccagctcccg ggtgtcggag gcgtgccagc cgatcttgcg gtaggccttg
      241 ccggcggaga acccctcggt tccggtgggg acgatgatcg aggagatctc cttgtggccg
      301 ccgggctcgg tgccggtgac ggcggtgatc gtcaccaggc cggtgatcgg cgtgccggcg
      361 ttcgtgatga acgccttggt cccgttgatc acccactcgc cgccctcgag cgtggcggtg
      421 gtgcgggtgg cgccggcatc ggacccgccg tccggctcgg tcagcccgaa cgcgccgagg
      481 atctcggcgc ggcacatcgg gacgagccac cgctccttct gcgcctcggt cccgaaacgg
      541 tagatggggt tggcgcccag accgaccgcc gcctcgagcg tgatcgccac cgacgagtcg
      601 acccgggcga gctcctcgat cgccaggcag aacgacagga agtcggcgcc ctgcccgccg
      661 taccgctcgg ggaacgggag cccgaagagc cccagctcgc ccatctgctt gacgatgtcg
      721 agagggaact cctcggccgc gtccatctcc tcggcccgcg gggcaaccac ctcgtccgcg
      781 aaacgccgca cggttcggcg gaacgcctcc tgctcctcgg tgagcgcgaa gtccacgggg
      841 cggagtgtac cggggcgctc agcgctaccc ggtttccgtc accgagaccg gcagcggcgg
      901 cagggcccag ccggagcgtt ccatggtctt ccgggcccgc ttgaggtcgg cggagtcgag
      961 ggtgtcctcc ccataccgac gggccaggtc cgtcgccaga cgggcccagg cgttcacgta
     1021 cggggtgccg gtgtagaact cgagcgcctt gccgaaggac tcgcgcgcgc cctggcgatc
     1081 gcccagcagc cagagcacct ccccacgcgc ttcgaggagg ctggcctcgc cgccgaactc
     1141 atccggcgac gtcgcgcgat ccgccaggcc gagatgacgc tcggcccgag acgcgtcgcc
     1201 gcgctgcgcg gcgacgagcg ccagggcctc gtggatgaag cccaggtagc ccttctcctc
     1261 gagtgcctcc atgacgccct gcgccttctt caggtcgcgc tcggcggcgt cgaggtcgcc
     1321 ggtgaccagc cggacgtgcc cgcggtcgcc gatggaggcg gcccactccc accgcgcgcc
     1381 gagttcgtcg aagaggacga tggcgcggtc gagcggttcg atcgcctccg cgtagcgccc
     1441 cgtccgccgg tacgagttcc cgatgtgctg cagcgccacg gcgctcgaga agcgatcctc
     1501 accctcctcc ccgagccgga gggcctgctc cgccaggcgc tgggcctcgc gttcgtcgcc
     1561 ggtggaagag accgtcgagc agaggctgcc gagcgcgcgc gcttcggccc agcggtcgcc
     1621 gttccggtga gcgatctcca gcgcctcgcg ccactcgcgc tcggcggcct ggggatcgcc
     1681 gcgccaggag tacacccagg cggccatcag cttgacccgg gccgtcaccc acgggtcgtc
     1741 catggcgtcg gccgccgcgc gggcgcggtc gaacagcgcc tcggccccgt ccacgtcgcc
     1801 gtccacgttc agcacgatgt cgccgaggaa ccgcgacgcg tgcgcctgca cccgcaggct
     1861 gtcgggcgcg atctccaggg cccgttccag gttggcccgg gcgatcgagt actccgccag
     1921 ccagtactgc gactccccga tccccgccag gatgccggcc tcgtcctcgc cccaccgctc
     1981 gttcggcccg gcgagcgcca gcgcccgctc gtagaggtcg atcgagccgc ggaagtccat
     2041 ccgtcgccgc aggcggtcgc cggcctcgcc gagcgcgtgc ttcgcccggt cggccggggt
     2101 gcggtcggtg ggatcgaggt ccagcgcgtt gcgcgcggcc tgctccaggt ggtaggcgag
     2161 cgcctccggg aaccgccgct tcaggtcggg ctggtccagc cggtcggcgg ccgcggcgtg
     2221 caggcggacc cgctcgcgct tcggcaggct ttcgtaggcc acgtcgcgca ggaactggtg
     2281 tcggaagcgc caggcgtcct tcacccggtc gtcgggcacc aggatctcct cctcctccaa
     2341 ctcgcggatg gcctgctcgg tgaccgcggc cacgagggcg agttcggtgc ggtgcaccgg
     2401 gggacggaag acggacatct tgcgggccag gtcgcgggcc ggctggttca ggtggtcgag
     2461 gcgggaggcc accaccgcct ggacggtcat cggcagcggc gcgcgccgcc actccccctc
     2521 ccggctggcg tgcatcagca tcccggtggt ctcgacgatg aagaacgggt tgccgccggt
     2581 ctgctcggcc accgcccgcg cggtatccag gtcgatggcc tcgccgccag aggcgagcgc
     2641 cagctcggcg ctctgctccg ggtcgagcgg gtcgaggcgc agcgtcaccg cgtcgagtcc
     2701 ggagccccac tcccgccgat gctcgaggag gtcctcgcgc gccacggtga ggacgaagag
     2761 cggcgccgcg ccgatccggc ggaccacgtg gtcgaccagg tcgagcatgg ccggcttcgc
     2821 ggcgtgcgcg tcctccagga tcagggccac cggcccccgt cccgacatcc cgcgcagcag
     2881 cgtcttgaac ccgtgctgga tctcctgctg cgggaacgcg ccctcgacaa gggtacggtg
     2941 cgcggccccc gggtcctgct ggggccg
//
LOCUS       DASPWO010000214         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1053056, whole genome shotgun sequence.
ACCESSION   DASPWO010000214 DASPWO010000000
VERSION     DASPWO010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053056"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VEO00_04760"
     CDS             complement(<1..791)
                     /locus_tag="VEO00_04760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYU57345.1"
                     /translation="MNVLVVTNDFPPRVGGINYYVGHIARRFPPGEVTVFASAWEGAR
                     AFDADHPARVVRWPARTMLPTPAVLDRVAGLARETRADVVLFGAAFPLALLGPAIRRR
                     TGVPYAAFTHGWEVGAASVPGGALPLRRIASGAALLTAVSGWTRDVISRAVGPGVRIE
                     LLPPGIDRERFHPGVDDRPVRERHGLGDRPVVCCVSRLVPRKGQDQVIRALPRIAQEF
                     PDVAFLVVGAGPYGHRLRSLAAERGVAGRVVFAGEVPYEELPAYFR"
     gene            complement(852..1526)
                     /locus_tag="VEO00_04765"
     CDS             complement(852..1526)
                     /locus_tag="VEO00_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57346.1"
                     /translation="MGTTPAPPAPSPSPMPPARRGAPPGVRYVPPALYLVAILCFFLP
                     FVTVSCASVQIAEVTGFDLLLNKDIETPPGLEDLVPGGTTPSDVSVQEIFRDDPRPFA
                     IAAAAAAVLALGLAFVPRRPVRVTGLILGVATAALVFSLRFRFHMDVPSAEDLPQGTP
                     DIFGGVNPITVTYGLGYWGAMLLALAAAGVQFWLVLRPPAGEGAAPAASALPTTAAPP
                     PAPAPG"
     gene            complement(1610..2254)
                     /locus_tag="VEO00_04770"
     CDS             complement(1610..2254)
                     /locus_tag="VEO00_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYU57347.1"
                     /translation="MSAVLDVTEETFEQDVLEASKTMPVVVDFWADWCQPCRILGPIL
                     ERLAAEPDAGWRLVRVDTDANPNLAAAFSIQSIPNVKAFLDGRLAGEFIGVQPEETIR
                     TWVGQFLPDPAQDVLAQARAAAARGRYDDARALLAPIADRFDADRLLKAIERVQAAMP
                     ELAEGVPEAGLERLLARAPEDEDARETMIAAFEILGDGDPATGEYRRRLANVIF"
     gene            complement(2352..>2769)
                     /locus_tag="VEO00_04775"
     CDS             complement(2352..>2769)
                     /locus_tag="VEO00_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYU57348.1"
                     /translation="IELFRGPAASWFFHALGGFSVRRQVADLRAMEVALAVVRSGRLL
                     GMFPEGTRSPDELLPFLPGAAWVALAEGVPLVPAAIRGTVESLPRGRVVPKRTDVRVA
                     FAPPIEVAREDDPVRRRARAVEITDGLRADVVRLLG"
BASE COUNT          411 a         1029 c         1024 g          305 t
ORIGIN      
        1 cggaagtacg ccggcagctc ctcgtagggg acctccccgg cgaagaccac ccgaccggcg
       61 acgcccctct ccgcggccag ggaccggagc cggtgcccgt acgggccggc gccgaccacc
      121 aggaaggcga cgtcggggaa ctcctgggcg atccgcggca gcgcccggat cacctgatcc
      181 tggcccttgc gcggcacgag gcgcgagacg cagcacacca ccgggcgatc ccccagcccg
      241 tggcgctcgc gaaccgggcg gtcgtccacc ccggggtgga atcgctcccg gtcgatcccc
      301 ggcgggagca gctcgatccg aacgcccggc ccgacggccc ggctgatcac gtcccgggtc
      361 caccccgaga ccgccgtcag gagcgcggct ccggaggcga tgcggcgcag cgggagcgcg
      421 ccgcccggga cgctggcggc gccgacctcc cagccgtgcg tgaacgccgc gtacgggacg
      481 cccgtccggc gccggatcgc cgggccgagg agcgccaggg ggaaggccgc cccgaacagc
      541 acgacgtcgg cccgggtctc gcgcgccagt ccggcgacgc ggtcgaggac cgccggcgtc
      601 ggcagcatcg tccgcgccgg ccagcgcacc acgcgcgccg gatggtcggc gtcgaacgcc
      661 cgggcaccct cccaggccga cgcgaacacg gtgacctcgc ccggcgggaa gcgccgggcg
      721 atgtgaccga cgtagtagtt gatcccgccg acgcgcggcg ggaagtcgtt ggtgacgacg
      781 aggacgttca atgggaggcc tccgggtccg ccgctcgacg cggcgccggc ggcgattcta
      841 cggcccagcc gtcagccggg cgcgggtgcc ggcggcggtg cggccgtcgt cgggagcgct
      901 gaggcggccg gagcggcgcc ctcgccggcc gggggccgca gcacgagcca gaactgcacc
      961 ccggccgcgg ccagagcgag cagcatcgcc ccccagtacc ccagcccgta ggtcacagtg
     1021 atcgggttca cgcccccgaa gatgtccggc gtgccctgcg gcaggtcctc ggcgctgggc
     1081 acatccatgt ggaacctgaa gcgcagcgag aacacgagcg cggcggtggc aacgccgagg
     1141 atcagccccg tgacccgcac gggacggcgc ggcacgaacg ccaggccgag ggcgaggacg
     1201 gcagccgcgg ctgcggcgat ggcgaacggc ctggggtcgt cgcggaagat ctcttggacg
     1261 gaaacgtcgc tcggcgtggt gcctcccggg acgaggtctt ccagcccggg cggcgtctcg
     1321 atgtccttgt tgaggaggag gtcgaacccg gtgacctcgg cgatctggac actggcgcag
     1381 gagacggtca cgaacggcag gaagaagcag aggatcgcca ccaggtagag cgcgggggga
     1441 acgtagcgga ccccgggcgg agcgccgcga cgggccggcg gcatcgggga cggcgacggg
     1501 gcgggcggag ccggcgtggt tcccacggat cctcctctcc ggcgggcggt gggtgaccgc
     1561 cgagcacggt agcccgcccc ccagaggggg tcaagcgccg gtcggccggt cagaagatga
     1621 cgttcgccag gcggcgccgg tactcgccgg tggcggggtc gccgtcgccg aggatctcga
     1681 acgcggcgat catcgtctcg cgggcgtcct cgtcctcggg cgccctggcg agcagtcgct
     1741 ccagcccggc ctccgggaca ccctcggcca gctccggcat cgcggcctgc acccgctcga
     1801 tcgccttcag cagccggtcc gcgtcgaagc ggtctgcgat cggagcgagg agggcgcggg
     1861 catcgtcgta ccgtccccga gccgcggcgg cccgagcctg cgccaggacg tcctgcgccg
     1921 ggtccggcag gaactgccca acccacgtcc ggatcgtctc ctcgggctgc acgccgatga
     1981 actcgccggc aagcctcccg tcgaggaatg ccttcacgtt cggaatgctc tggatgctga
     2041 aggcggcggc gaggttcggg ttcgcgtcgg tgtcgaccct caccagccgc cagccggcat
     2101 cgggttcggc ggccagccgc tcgaggatcg ggccgaggat ccggcagggc tggcaccagt
     2161 cggcccagaa gtccacgacc acgggcatcg tcttggacgc ctcgagcacg tcctgctcga
     2221 aggtctcctc ggtgacgtcg agtacggcgc tcatctgtcc atgtggggag gagacccacc
     2281 ccacccccca cccgtcgagt catgccgtgg agcgctcatc ccgtcgaacc tagcggaagc
     2341 cgcgggaacg gttaccccag cagccggacc acgtcggccc gcagcccatc ggtgatctcc
     2401 acggcgcggg cccggcggcg gacggggtcg tcctcgcgcg ccacctcgat cggtggggcg
     2461 aaggccacgc gcacgtccgt gcgcttcggc accacccgcc cgcgcggcag ggactcgacc
     2521 gtcccgcgga tcgctgccgg cacgaggggc acaccctcgg cgagcgccac ccacgccgcc
     2581 cccgggagga atggcaggag ctcgtcgggg cttcgcgtgc cctccggaaa catcccgagc
     2641 agccggcccg agcgtacgac ggccagcgcg acctccatcg cccgcaggtc ggccacctgg
     2701 cggcgcaccg agaagccgcc cagagcatgg aagaaccacg acgccgccgg cccacggaac
     2761 agctcgatc
//
LOCUS       DASPWO010000215         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_624078, whole genome shotgun sequence.
ACCESSION   DASPWO010000215 DASPWO010000000
VERSION     DASPWO010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624078"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VEO00_04780"
     CDS             <1..379
                     /locus_tag="VEO00_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU57349.1"
                     /translation="WLASMPNGLDTRVGERGEALSVGERQLVSLSRAQVGDPGLLILD
                     EATSAVDPAMERRISEALRRLSAGRTSITIAHRLSTAEGAETILVFDGGRVVERGRHD
                     ELVAAGGVYAGLYRSWLGNVRAP"
     gene            complement(416..1777)
                     /locus_tag="VEO00_04785"
     CDS             complement(416..1777)
                     /locus_tag="VEO00_04785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HYU57350.1"
                     /translation="MRRPRSPMTVAGAVERIVGPDLLIAVETYDGGRIGPADARATLV
                     VRSPDALRRILTAPGELGLGRAYVAGDLDVEGDIYAALASLRERLPRVRLGPSQLVAL
                     ARLVGLDGLRPLPPPPEEARLRGRRHSKGRDAQAIAHHYDVSNRFYELVLGPSMTYSC
                     AVWPRPDATLEEAQAAKYELICRKLDLGEGMRLLDVGCGWGGMAMHAARHHGASVVGV
                     TLSRRQVEWAQKEVADAGLADRVEIRLQDYRDVLDGPFDAISSIGMFEHVGLRNLGLY
                     FRHLYRLLRPGGRLLNHGISRPARPGARTRAGVPVTHPRLARRSFIDRYVFPDGELHE
                     VGAVVSTMQAAGFEVRHLESLREHYALTLRAWVANLELNWEEAVAEVGAARARVWRLY
                     MAASALNFEAGRTQVHQVLGVRADRGRFPANHGPNTDPLRPVEGSSPAAAGPRAVGSR
                     LDP"
     gene            complement(2276..>2465)
                     /locus_tag="VEO00_04790"
     CDS             complement(2276..>2465)
                     /locus_tag="VEO00_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57351.1"
                     /translation="FSVCRREFQRLADLELPAAADKRMVLQDLESRIGVVGFDDGPTV
                     KGSFGRSLTRPVPIDRLE"
BASE COUNT          396 a          828 c          864 g          377 t
ORIGIN      
        1 gtggctggcg tcgatgccga acggcctgga cacccgcgtc ggcgagcggg gcgaggcgct
       61 gtcggtgggg gagcgccagc tcgtgtcgct ctcgcgcgcg caggtcggcg accccggcct
      121 gctgatcctc gacgaggcga caagcgcggt tgaccccgcg atggagcgac ggatttccga
      181 ggcactccgg cggctttcag ctggccggac gtcgatcacc atcgcgcatc ggctgtcgac
      241 cgccgagggc gccgagacga tcctcgtgtt cgacgggggg cgcgtcgtcg agcgcggccg
      301 tcacgacgag ctggtcgcgg ccggcggcgt gtacgcgggg ttgtaccgaa gctggctcgg
      361 caacgtccga gcgccctgag ggccggtcta gggtcctcga accgcacatt cgagttcaag
      421 gatccaaccg agacccgacc gcgcgcggtc ccgcggctgc gggactcgaa ccctcaacgg
      481 gccgaagtgg gtccgtattg ggtccgtgat tggcggggaa acggccccgg tcagctcgga
      541 ccccgaggac ctggtgcacc tgggtccggc ccgcctcgaa gttgagggcg ctggcggcca
      601 tgtacagccg ccagacccga gcccgtgccg cgccgacctc ggccacggcc tcctcccaat
      661 tcaactcgag gttggccacc cacgcccgga gcgtcagcgc gtagtgctct cgcaggctct
      721 ctaggtgccg aacttcgaac ccggcggcct gcatggtgga gacgaccgcg ccgacctcgt
      781 gcagctctcc gtcggggaac acgtatcggt cgatgaagct gcgccgcgcg aggcgcgggt
      841 gagtgaccgg cacgccggcc ctcgtgcgcg ccccgggcct ggctggccgg ctgatcccgt
      901 ggttcaacaa cctgccgccc ggccggagca ggcggtagag atgccggaag tacagcccga
      961 ggttccgaag gcccacgtgt tcgaacatgc cgatcgagga gatcgcatcg aacgggccgt
     1021 cgaggacgtc ccggtagtcc tggaggcgga tctccacccg atcggccagg ccggcgtcgg
     1081 ccacctcctt ctgggcccac tcgacctggc gcctggagag cgtgaccccg acgaccgacg
     1141 cgccgtggtg cctcgccgcg tgcatggcca tcccgcccca cccgcacccc acgtcgagga
     1201 gccgcatccc ctcgccaagg tcgagctttc ggcagatcag ctcgtacttg gcggcctgcg
     1261 cctcctcgag cgtcgcgtcg ggccgcggcc acacggcgca cgagtaggtc atggatggtc
     1321 cgaggaccag ctcgtagaac cggttcgaca cgtcgtagtg gtgggcgatg gcttgcgcgt
     1381 cccggccttt cgagtgccgc cggccgcgga gcctggcttc ctccggcggc ggcgggagtg
     1441 gccggaggcc gtcgaggccc accaaccggg cgagagccac cagctgggac ggtccgagcc
     1501 ggacccgcgg caggcgctct cggagggacg cgagcgccgc gtagatgtcg ccctcgacgt
     1561 cgaggtcgcc ggcaacgtag gcccggccga gcccgagctc gcccggagcg gtcaggatcc
     1621 ggcggagcgc gtcgggcgaa cgaacgacca gcgtggcccg ggcgtcggcg gggccaatcc
     1681 ggccaccgtc gtacgtctcg accgcgatta gcaggtcggg cccgacgatc cgctcgacgg
     1741 cccccgcgac cgtcatcggc gatctcggtc tcctcatgtc gtttgaaata ggtgcgctca
     1801 gtgatgctat tcggttttca ccgcggcatt gacgtcctcg atcgactgcg ggaaacgcca
     1861 ctctgcgctt ggtccgaagg ttgacaaggt cgcccaggac cttaaacgga tggggcccgg
     1921 gtgcagacca attttctcag cgcctcttta cgccccttgc ctaacggagc gccagattca
     1981 ggaaagggag tatgaggagg gtgcctgatg cgtctcgatc ggtccgtggg gttgaccccg
     2041 cccagcattg atgacggaac ggccgtgtcg gagtggagga ttatcctgat gcgatcagat
     2101 cacgcgatcg ggaaagcgcg atcgggaagg agggaccaag accaggttct tggtcaccta
     2161 ccacggaagc gagatgcctc acgaaccgga ggccatcgcc cagggtcgcg acgcctttat
     2221 ccagtgggcc ggcaagacag gagccgccct cgtcgaacct ggcgatccgg tcaggtcatt
     2281 caagacgatc gatcggaacg ggacgcgtga gggagcggcc gaaggaccct ttaacggttg
     2341 gtccgtcatc gaagccgacg acgccgatgc ggctctccag atcttgaagg accatccgtt
     2401 tatcggccgc ggcgggcagc tccaggtcag cgagcctttg gaattcccgc cggcagactg
     2461 agaat
//
LOCUS       DASPWO010000216         8714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1132719, whole genome shotgun sequence.
ACCESSION   DASPWO010000216 DASPWO010000000
VERSION     DASPWO010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8714
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132719"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VEO00_04795"
     CDS             complement(<1..310)
                     /locus_tag="VEO00_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013475161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU57352.1"
                     /translation="MAELPTGTVTFLFTDIEGSTRLLQALGDRWPPVLEDHNRLLRDA
                     IRGAGGVDLRTEGDAFFAVFGSAPAAVQAAAAAQRALAAHPWPAGATVRVRMGLHTGE
                     G"
     gene            507..578
                     /locus_tag="VEO00_04800"
     tRNA            507..578
                     /locus_tag="VEO00_04800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:539..541,aa:Val,seq:cac)
     gene            616..1386
                     /locus_tag="VEO00_04805"
     CDS             616..1386
                     /locus_tag="VEO00_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57353.1"
                     /translation="MRRASLASFLVSVALLGAACSGNDVTIGVPSGSPSTTSPAATPS
                     PSPSPSPVPNPADAELAGLLRPGTVEVGHLFPELNGVPPAEIVISSRDTTSADAFPPQ
                     PYLDVFSWNPGVARWVNVFDATTFLNPAGPNPGPVILTEPGLGQAVDFLDALELDPDI
                     GFELLVGIQISGAAPGPLHLWVFSWPSEAFSVDFYDETERGGIVTVDPARPAVIIETG
                     KYGPNDPGCCPSEIETRTIAFDPAAGEIRIVDRVTRPA"
     gene            1392..4493
                     /locus_tag="VEO00_04810"
     CDS             1392..4493
                     /locus_tag="VEO00_04810"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HYU57354.1"
                     /translation="MADLFVSYSRKDLEFVRGLHRLLTARGKDVWVDWEDIPPTAEWE
                     EEIARAVDSADAFLFVLSPDSVASRVCARELERAIESHKRLIPVLFREVAEPVPDALA
                     RLNWIYTRDGDDLAHAVNTIIGAAETDLERARAHSRLLVRASEWEARGRDASYLLRGS
                     DLVDAEALVTALVEGTELESTAQQSSYVVASRQAATRRQRVTGGAVALGLVVAVVLAV
                     LALIQRNQAVREGARAERQAQIAQSRELAALAEGQLSIDPELSILLAREGAKLAPTNQ
                     IADALRAAIPRSHLRALLSGHSDAVSVAEFGPDGSQVVTGSWDGTVRLWNARSGAAVA
                     TVARDLGQVHDVAFSPDGRRVAAAVFGRTFLWDLDARGDEVPLAGASSDSEINVVAFS
                     PSGALLASGSGSGEVKVWDANDGSLVASMRDAATHVGALAFGPDGRTLASAPGHFITG
                     APNILTSLAGPPRLWDVARGRLVATLEISEADVVTDIAFAPDGRLVVGATSKGARLWD
                     LHGRLVRTMPAPGTFVVGVPGTGNLLATGGLDGVARVWDSRTGAELATFRGHDGAVLS
                     VAFRHDGKFVVSTGSDRTARVWVALTGREASVLRGHEGWVLGGSFEPDGTRVVTASLD
                     RTARVWEPGVQEGIVELKGHLGPVTVATFAADGTRLATGELQEAPLVRVWDPETGREV
                     ATTESHHEVVRSIAFSPGGELLLSLSGGMDTRIHVADGRTGATVLLYDAQGIRGASSA
                     VWAPDGRRILSAGADGIARLWDARSGRTILTLDDRDEEPDRIALSAAAPSPDGTRIVT
                     AGSFATGPLDDPDIVGILSVWDGATGGRLRSRRDLPYPITTAAFSADGRTILTNSGNG
                     ATLWDPETLHAVTELRGHTAPVSVASFSPEGSRIVTASTFELDPVTFEPLPGDLNVRI
                     WDARSGTLLRTLPQPAAVRDVAFNPAGTRLAVATDLGGSVWDVAPGRQIAALPGLPEG
                     ASDAEFSPDGRYLALAGSDGIARIYSAETFAPLDALIQLADRRVTRGLTPDERRAFLH
                     EPG"
     gene            4550..6469
                     /gene="thrS"
                     /locus_tag="VEO00_04815"
     CDS             4550..6469
                     /gene="thrS"
                     /locus_tag="VEO00_04815"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HYU57355.1"
                     /translation="MARITLPDGSVREVGAETPAGDLLGRDAVAARLDGVLIDLSAPL
                     ERDGRLEPLTLDEHDGLHVLRHSTAHVLAQAVCDLIPGAHYAIGPPIEDGFYYDFDLP
                     QPLTPEDLEKVEARMREIIAADQLFVREELSRAEALERFADQALKREIIESLDPSEVG
                     SGDSVSVYVNDGWSDLCLGPHLPSTGRIGAFRLLSLAGAYWRGDEHRPMLQRVYGTAW
                     SSEDDLEAYLHRLEEAERRDHRRLGRDLDLFSFPDEIGAGLAVWHQRGAILRKELEDF
                     VRDIHIRHGYELVQTPHVARGGLWATSGHLAKYRQNMYPAMEEPGGGEYFLKPMNCPL
                     HVLIYRSRTRSYRDLPLRLSELGTVYRYERPGTLHGLLRIRGLTIDDAHIFCRPDQLV
                     DEIVKVFEMTLEVHRTFGFEEPKVELSTKPGEAIGTEEMWRRATETLRAALDVSGMAY
                     SDAEGEGAFYGPKVDFHFRDAIGRLWQLTTVQCDFALPERFGIEYMTEENTRERPVMI
                     HRALLGSVERFLGVLTEHYAGAFPLWLAPEQVRVVPIADRHAAHAEALAARLREAGLR
                     AGVDDGRETVSKKVRDAQLLKVPYTLVVGDKEIEGGTVAVRDRRGNETRGVAFTEFMA
                     RVQEEVRSRALETAG"
     gene            6469..6957
                     /locus_tag="VEO00_04820"
     CDS             6469..6957
                     /locus_tag="VEO00_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HYU57356.1"
                     /translation="MERLWTPWRMEYVKGADLADGCFLCDAPAAGDDGATYILHRGEN
                     AFVILNIYPYNSGHLMVAPYRHAADIEELEPDEAVEGWSLLLRSVRVLREAMAPQGFN
                     IGMNLGRVAGAGVPDHLHFHVVPRWGGDTNFMPVTGAAKVLPELLDETYARLKPLFEE
                     SA"
     gene            6954..>8714
                     /gene="glgP"
                     /locus_tag="VEO00_04825"
     CDS             6954..>8714
                     /gene="glgP"
                     /locus_tag="VEO00_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HYU57357.1"
                     /translation="MTRDGRGDVAGAVRALADGIPPALGSLAELAYNYRWAWTSGGAD
                     FASIDPLGWEREGGNPVRLLSGASGHALARAAADDGLVRRAAAEAAALREELGRPAAD
                     GPVRPERPIAFLCTEFGVHPSLPIYAGGLGVLAGDLLKEASDRAVPMVGVGLLYRQGY
                     FHQRMDDSGWQHEFWFDADPERMPAALVTGQDGEPVTVAVEVRGRPVAVQIWRVDVGR
                     TPLFLLDADRPENHPIDRWITSRLYIRERHVRLAQYALLGIGGIRALRTLGIEPGVVH
                     LNEGHASLAAFELIREQVDGGVPFAEALERARSMTVFTTHTPVAAGNESYPTADVLEA
                     LTDLRPTFGADLQPLLELGRARPEDAEEPLGLTTLALRVSRTSGGVSRRHGGVARAMW
                     QHLFPGHEVGEVPIGHVTNGVHLPTWMAPPMRALLERHLGPGFARAASDPAVWARVEE
                     IPDEALWAVRGELRQELVAHARERAAADRLARGNSLAFAEAAARALDPDTLTLGFARR
                     ATAYKRIHLLSLDPERALGLLSDPHPIQLLLAGKPHPADEEGKRLIQRLFELRTAPAV
                     ADRVAFLEDYQMGMAAKLIAG"
BASE COUNT         1179 a         3084 c         3195 g         1256 t
ORIGIN      
        1 cgccctcgcc ggtgtgcagt cccatccgca cgcggacggt ggcccccgcc ggccacgggt
       61 gggccgcgag ggcacgctgg gccgcggcgg cggcttgcac cgcggccggg gcgctgccga
      121 acaccgcgaa gaaggcgtca ccctcggttc gcaggtcgac cccgcccgcc ccgcggatgg
      181 catcgcggag cagacgattg tggtcctcga ggaccggcgg ccaccggtcg ccgagcgctt
      241 ggagcagccg tgtggacccc tcgatgtcgg tgaacaggaa ggtgacggtc ccggtgggca
      301 gctcggccat cggccgcccg agcctacgcg atggccatgg cgcccgcctg ctcggaacgg
      361 ttggcggttt tccctcccgt ctgtcaaact ctggcgcgtc gggccttcgg gttcggcacg
      421 ggcggcgggg cgccgcgggc acgccctgcc gcccactctt tccggccctc cgcggcagcc
      481 ccgtttgcta tcgtggtcgt cctcccgggc gcgtagctca gcgggagagc gctcgcttca
      541 cacgcgagag gtcgcaggtt caaatcctgc cgtgcccacc gattcgggcc cgggcccggg
      601 atagcatcgg ccgacatgcg ccgcgcctcg ctcgcctcgt tcctggtctc cgtggcgctc
      661 ctcggcgcgg cctgttccgg gaacgacgtg acgatcggcg tgccgtccgg ctcgccctca
      721 accacctcgc ccgccgccac cccgtccccg agcccgtcgc cctcgccggt cccgaacccc
      781 gccgacgccg agctggcggg gctgctgcgg ccggggaccg tggaggtcgg gcacctcttc
      841 cccgagctga acggggtccc accggccgag atcgtcatct cctcccgcga cacgacgtcg
      901 gcagacgctt tcccgccgca gccctacctc gacgtcttct cgtggaaccc cggggtggcg
      961 cggtgggtga acgtcttcga cgccaccacg ttcctgaacc cggccggacc gaacccgggc
     1021 ccggtgatcc tgaccgagcc cgggctgggc caggccgtcg acttcctgga cgccctggag
     1081 ctcgaccccg acatcgggtt cgagctgctg gtcggcatcc agatctccgg cgcagcgccc
     1141 ggaccgctgc acctctgggt gttctcgtgg ccgtcggagg cgttcagcgt cgacttctac
     1201 gacgagaccg agcgcggggg gatcgtcacc gtcgacccgg cccgcccggc cgtgatcatc
     1261 gagacgggga agtacgggcc gaacgacccc ggctgctgcc cgagcgagat cgagacgcgc
     1321 acgatcgcct tcgaccccgc cgcgggcgag atacggatcg tcgatcgggt cacccggccg
     1381 gcctgaccga catggcggac ctgttcgtct cctattcgcg gaaggacctc gagttcgtgc
     1441 gcgggctcca tcgcctcctc acggcgcgcg gcaaggacgt ctgggtggac tgggaggaca
     1501 tcccgccaac cgcggagtgg gaggaggaga tcgcgcgagc ggtcgattcg gccgatgcct
     1561 tcctgttcgt gctcagcccg gactccgtgg cttcccgggt ctgcgctcgc gagctcgaac
     1621 gggcgatcga gtcacacaag cggctcatcc cggtcctgtt cagggaggtc gccgagccgg
     1681 ttcccgacgc gctcgctcgc ctgaactgga tctacacacg ggacggcgac gacctcgcgc
     1741 atgcggtgaa caccatcatc ggcgcagcgg agaccgacct ggaacgcgcg cgcgcacact
     1801 cccgcctcct cgtgcgcgcg tcggagtggg aggcccgtgg acgggacgcg agctacctgc
     1861 tgcgaggcag cgacctcgtc gacgcggaag ccctggtcac cgccttggtc gaaggaacgg
     1921 agctcgaatc gacggcgcag cagagcagct atgtcgtggc gagccgtcag gcggcaaccc
     1981 ggcggcagcg ggtcaccggc ggtgccgtgg cgctcggact ggtggtggcg gtggtcctcg
     2041 cagtgctcgc tctgatccag cgaaaccagg cggtgcgcga gggcgcacgg gccgaacggc
     2101 aagcgcagat cgctcaatcc cgggaactgg cggccctcgc ggagggccag ctgtcgatcg
     2161 acccggagct ctcgatcctc ctggcacgcg aaggggcgaa gctcgcgccc accaaccaga
     2221 tcgccgatgc cctccgtgcg gcgatcccgc ggtcgcacct ccgggcgctg ctgtcggggc
     2281 attcggacgc ggtgtccgtc gccgagttcg ggcccgacgg ttcgcaggtc gtgacgggga
     2341 gctgggatgg gaccgttcga ctgtggaatg cccgctccgg cgcggccgtc gccacggtgg
     2401 ctcgcgacct cggtcaggtt cacgacgtgg cgttcagccc cgacggacgg cgggtcgccg
     2461 cggcggtgtt cggccgcacg ttcctgtggg acctggatgc ccggggcgac gaggtccccc
     2521 tcgccggagc atcctccgat tccgagatca atgtcgttgc gttcagtccg agcggcgccc
     2581 tcctcgcctc tggaagtggc tcgggcgagg tgaaggtctg ggatgcgaac gatggaagcc
     2641 ttgtcgcgtc gatgagagac gccgcaaccc atgtcggggc gctcgccttc ggtccggacg
     2701 gccgcacact cgcaagcgcc cccggccact tcatcaccgg cgcgccgaac atcctgacgt
     2761 ccctggccgg tccaccgcgg ctctgggacg tggcgagggg gcggctcgtg gccacgctgg
     2821 agatctcgga ggcagacgtc gtcacggaca tcgcgttcgc tccggacggt cgactcgtgg
     2881 tcggggcaac gtcgaagggc gcgcgtctct gggatctgca cggtcggctc gtacggacga
     2941 tgcccgcccc gggcacgttc gtcgtgggcg tgccgggcac cgggaacctc ctggcgacgg
     3001 gtggtctgga cggcgtcgcg cgggtgtggg actcccggac cggggcggag ctcgcgacgt
     3061 tccgcggaca cgacggagcc gtcttgagcg tcgcattccg ccacgacggg aagttcgtcg
     3121 tcagcaccgg atcggaccga acggctcggg tctgggtggc cctcaccggc cgcgaggcgt
     3181 cggtccttcg agggcatgag gggtgggtgc tggggggctc cttcgagccc gatggaacgc
     3241 gggtcgtgac cgcgagcctc gaccggaccg cccgcgtctg ggagccgggc gtccaggagg
     3301 ggatcgtcga gctcaagggc cacctgggtc ccgtgacggt cgcgaccttc gccgccgacg
     3361 gcacgcgcct ggccaccgga gagctccagg aggcgccact ggttcgggtg tgggacccgg
     3421 agaccgggcg agaagtggcc acgaccgaga gccaccacga ggtggtgcgg tcgatcgcct
     3481 tcagtcccgg cggcgagttg ctcttgagcc tgagtggcgg gatggacacg cggatccacg
     3541 tcgccgacgg gcgaaccggt gcgacggtcc tcctctacga tgcgcagggg atccgcggtg
     3601 cctcgtcggc ggtctgggca cccgacggcc gacggatcct gtctgccggc gccgatggca
     3661 tcgcgcggct gtgggacgcg cgttcggggc ggacgatcct caccctcgat gaccgggatg
     3721 aggagcccga tcggatcgcg ctctccgcag cagcaccgtc gcccgacgga acgaggatcg
     3781 tcaccgccgg gagcttcgcg acgggaccgc tggacgaccc cgacatcgtc ggcatcctga
     3841 gcgtctggga cggcgccacc ggcgggcggc ttcgctcgcg gcgcgacctc ccgtatccga
     3901 tcaccacggc tgccttcagc gccgacggac gcacgatcct cacgaacagc gggaatgggg
     3961 caacgctgtg ggatccggag acgctgcacg cggtgacgga gctgcgcggt cacacggctc
     4021 cggtatccgt cgcgtccttc agtcccgagg gcagccgcat cgtcaccgcg agcacgttcg
     4081 agctcgaccc cgtcactttc gaaccgctcc ccggtgacct gaacgtcagg atctgggatg
     4141 cgaggagtgg gaccctgctc cgaaccctgc ctcagcccgc cgccgtccga gacgttgcct
     4201 tcaacccggc gggaacgagg ctcgcggtgg cgacggatct cggcggaagc gtgtgggatg
     4261 ttgcccccgg acggcagatc gcggcgcttc ccggcttgcc cgagggggca tccgatgccg
     4321 agttctcgcc ggacggtcga tacctcgcgc tggcggggtc agatggcatc gcccggatct
     4381 actcggccga gacgttcgcc ccgttggatg cgctcatcca gttggcggat cggcgcgtca
     4441 cccgcgggct gacacccgac gaacggcggg ccttcctgca cgaacccggt tgacgcccct
     4501 gcccggcggc ccgggccgac cgcgcccccg gagccggtac gatgggccga tggcgcggat
     4561 cacccttccg gacggctcgg tccgcgaggt gggggcggag accccggccg gcgacctgct
     4621 cgggcgggat gccgtcgccg cccggctcga cggcgtgctg atcgacctgt cggcgccgct
     4681 cgagcgcgac gggcggctcg agcccctgac gctcgacgag cacgacggac ttcacgtgct
     4741 ccgccactcg accgcgcacg tcctggccca ggcggtgtgc gacctgatcc ccggcgcgca
     4801 ctacgcgatc gggccgccga tcgaggacgg cttctactac gacttcgacc tgccacagcc
     4861 gttgacgccg gaggacctgg agaaggtcga ggcgcgcatg cgcgagatca tcgcggccga
     4921 ccagctgttc gtgcgcgagg agctgtcgcg cgcggaggcg ctcgagcgct tcgcggacca
     4981 ggcgctgaaa cgggagatca tcgagagcct cgacccgtcc gaggtcggga gcggcgattc
     5041 cgtctccgtc tacgtcaacg acggctggtc ggacctgtgc ctcgggccgc acctgccctc
     5101 gaccggccgg atcggggcgt tccggctgct gtcactcgcc ggtgcgtact ggcgcggcga
     5161 cgagcaccgc ccgatgctgc agcgggtcta cggcaccgcc tggtccagcg aggacgacct
     5221 cgaggcctac ctgcaccggt tggaggaggc ggagcggcga gatcaccgcc gtctcggccg
     5281 cgacctcgac ctgttctcgt tcccggacga gatcggggcg gggctcgcgg tgtggcacca
     5341 gcgcggggcg atcctgcgca aggaactgga ggacttcgtc cgcgacatcc acatccgcca
     5401 cggctacgag ctggtgcaga ccccgcacgt ggcgcgcggt gggctctggg ccacctccgg
     5461 gcacctggcc aagtaccggc agaacatgta cccggcgatg gaagagcccg gggggggcga
     5521 gtacttcctg aagcccatga actgcccgct gcacgtgctg atctatcgat cgcgtacccg
     5581 ctcgtaccgc gatctcccgc tgcgcctgtc cgagctcggc accgtctacc gctacgagcg
     5641 gccggggacc ctccacgggc tgctccggat ccgcggcctg accatcgacg acgcgcacat
     5701 cttctgccgc cccgaccagt tggtggacga gatcgtcaag gtgttcgaga tgaccctcga
     5761 ggtgcaccgc acgttcggct tcgaggagcc gaaggtcgag ctctccacga agccgggcga
     5821 ggcgatcggg accgaggaga tgtggcgccg ggccaccgag accctgcgtg ccgcgctcga
     5881 cgtcagcggg atggcgtact ccgacgccga aggcgagggg gcgttctatg ggccgaaggt
     5941 ggacttccac ttccgcgacg cgatcggccg actgtggcag ctgaccacgg tgcagtgtga
     6001 tttcgcgctg ccggagcgct tcggcatcga gtacatgacc gaggagaaca cccgcgagcg
     6061 gccggtgatg atccaccggg ccctgctcgg ctcggtcgag cgcttcctcg gggtgcttac
     6121 cgagcactac gccggggcct tcccgctgtg gctggcgccc gagcaggtcc gcgtggtgcc
     6181 gatcgccgac cgccatgccg cgcacgctga ggcgctcgcc gcccggctgc gcgaggcggg
     6241 cctgcgcgcc ggggtggacg acggccgcga gacggtgtcg aagaaggtgc gcgacgcgca
     6301 gctgctgaag gtgccctaca ccctggtggt cggcgacaag gagatcgagg gcgggacggt
     6361 ggccgtgcgt gaccggcggg ggaacgagac gcgcggagtg gcgttcacgg agttcatggc
     6421 ccgcgtgcag gaggaggtcc ggtcgcgcgc actggaaacc gcggggtgat ggagcggctc
     6481 tggacgccct ggcggatgga gtacgtcaag ggcgccgatc tggcggacgg ctgcttcctg
     6541 tgcgacgcgc cggccgcggg ggacgacggg gccacctaca tcctgcaccg cggcgagaac
     6601 gccttcgtca tcctgaacat ctacccctac aactcggggc atctgatggt ggcgccgtac
     6661 cggcacgccg ctgacatcga ggagcttgag ccggacgagg ccgtcgaggg gtggtcgctg
     6721 ctgctgcggt cggtgcgggt gctgcgcgag gcgatggcgc cgcagggctt caacatcggg
     6781 atgaacctcg ggcgcgtcgc cggcgccggg gtccccgacc acctgcactt ccacgtcgtc
     6841 ccccgttggg gcggggacac gaacttcatg ccggtgaccg gggcggcgaa ggtcctccca
     6901 gagctgctcg acgagacgta cgcccggctg aagccgctgt tcgaggagtc cgcgtgacgc
     6961 gagacggtcg aggggacgtc gcgggggcgg tgcgggcgct ggccgacggc atcccgccgg
     7021 ccctcgggtc cctcgccgag ctcgcctaca actaccgatg ggcgtggacg tcgggcgggg
     7081 ctgatttcgc gtcgatcgat cccctggggt gggaacggga gggcgggaat cccgtccgtc
     7141 tcctgtccgg ggcctccggc cacgcgctcg cccgtgcggc cgcggacgac ggcctggtgc
     7201 gccgggccgc ggccgaggcg gcggcgctgc gcgaggagct cgggcgcccg gcggcggacg
     7261 gcccggtccg gcccgaaagg ccgatcgcct tcctgtgcac cgagttcggg gtccacccgt
     7321 cgctgccgat ctacgccggc ggcctcggcg tcctggccgg cgatctgctg aaggaggcgt
     7381 cggaccgggc ggtccccatg gtcggggtcg gcctgctcta ccggcagggc tacttccacc
     7441 agcggatgga tgactcgggc tggcagcacg agttctggtt cgacgccgac ccggagcgca
     7501 tgccggccgc cctcgtcacc ggccaggacg gcgagccggt caccgtggcg gtggaggtgc
     7561 gcgggcggcc ggtcgccgtg cagatctggc gcgtcgacgt cgggcggacg ccgctcttcc
     7621 tcctcgacgc cgaccgtccc gagaaccatc cgatcgaccg gtggatcacc tcgcgcctct
     7681 acatccggga gcgccacgtc cgcctggccc agtacgcgct gctggggatc ggcgggatcc
     7741 gggccctgcg cacgctgggg atcgagcccg gggtcgtgca cctgaacgag gggcacgcct
     7801 ccctggccgc gttcgagctg atccgcgagc aggttgacgg gggcgttccg ttcgcggagg
     7861 cgcttgagcg ggcccggagc atgacggtct tcaccacgca cacgccggtc gcggcgggca
     7921 acgagtcgta cccgaccgcc gacgtcctgg aggccctgac ggacctcagg ccgaccttcg
     7981 gcgccgatct gcagccgctc ctcgagctgg gccgtgcccg gccggaggac gccgaggagc
     8041 cgctcggact gaccacgctc gcgctccgcg tcagccgcac ctcgggcggc gtgagccgcc
     8101 ggcacggggg cgtcgcccga gcgatgtggc agcatctgtt cccgggtcac gaggtcggcg
     8161 aggtcccgat cggccacgtc acgaacggcg tgcacctgcc gacgtggatg gcgccgccga
     8221 tgcgcgcgct gctcgagcgc cacctcgggc cgggcttcgc gcgggccgcc tccgaccccg
     8281 ccgtctgggc gcgggtggag gagattccgg acgaagcgct gtgggcggtc cgtggcgagc
     8341 tgcggcagga gctggtggcc cacgcccggg agcgcgccgc cgccgaccgc ctggcccgcg
     8401 ggaactcgct cgcgttcgcc gaggccgccg cccgcgcgct cgacccggac accctgacgc
     8461 tgggcttcgc ccgccgggcg accgcctaca agcgcatcca cctgctcagc ctcgacccgg
     8521 agcgggcgct cgggctgctc agcgacccgc acccgatcca gctgctcctg gccggcaagc
     8581 cgcaccccgc cgacgaggag gggaagcggc tgatccagcg gctcttcgag ctgcggacgg
     8641 ccccggcggt ggccgatcgc gtcgcgttcc tggaggacta ccagatgggc atggcggcga
     8701 agctgatcgc cggc
//
LOCUS       DASPWO010000217         5825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_288418, whole genome shotgun sequence.
ACCESSION   DASPWO010000217 DASPWO010000000
VERSION     DASPWO010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288418"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3227)
                     /locus_tag="VEO00_04830"
     CDS             complement(<1..3227)
                     /locus_tag="VEO00_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYU57358.1"
                     /translation="MTNPEADRAFEIVGATLPGWPDPAVAIAASRAGATGLLNLEHTT
                     DLAAARSALARLGSFGRGSLGVKVRPGRGTSGDLAASLPPTINLVVVAGADALSARSA
                     IALVRAPGRRVLLECTSVEEARAALESGADGLVAKGHEAGGHVGEETTFILLQRLLRE
                     TDRPVLAYGGIGLHSAPAAYVAGCSGVVVDAQLALARESALPPRLRAALERMEGDETL
                     CVGAEIGDLYRVYQRPGMAALDALPQVEATLAAQPAGAARDRWREAIQARVGWERPEA
                     QLWPLGQDGAFAARLARRFHTVAGILDGLRASVVDHVRLARGQRALDRGAPLARSHGT
                     EYPILQGPMTRVSDTAAFAEAVARGGALPFLALALLRGPQVRALLQETGERLGDRPWG
                     VGILGFVPLELRQEQLEVIHDFRPPFAIIAGGRPDQAARLEANGIATYLHVPAPGLLR
                     MFVEDGARRFIFEGRECGGHVGPRTSFVLWQTMIDALLDALEAGIPADHLHIVFAGGV
                     HDDRSAAMVAAMAAPLVERGARIGVLLGTGYLFTEEAVAAGAIVEGFQREALGCAHTV
                     LLETGPGHSTRCVDTPFYETFREAKRRLLAEGKSPEELRRELEALNLGRLRIASKGIA
                     RDGEAEGSGPRYVEVDPERQHRDGMFMIGQLAALRKGVCRIADLHEGVSRGGARLLAG
                     LALPAGPAPRAVPEAARPCNVAIIGLGCLLPKAPDVPTFWANVLNKVDAITEVPKERF
                     DIDLYFDPDRKARDKVYSRWGGFLDDVPFDPMRYGIPPAALPSIDSLQLLSLEVARQA
                     LADAGYLDRDFPRARTSVILGVSGGLGDLGLGYGIRSNLPAVLGRAPAQVLERLPEWT
                     EDSFAGILLNVAAGRVANRFDLGGVNYTVDAACASSLAAAYLAVSELEAGTSDMVVVG
                     GVDTVQSPFGFLCFSKSQALSPRGRCRTFDAGADGIAISEGITMLVLKRLADAERDGD
                     RIYAVVKAVAGSSDGRGRSLTAPRPEGQTLALERAYARAGISPATVGLVEAHGTGTVV
                     GDAAEVAALSEVFQAAGAAPSSCAIGSVKSMVGHTKSAAGVTGMM"
     gene            complement(3313..3906)
                     /locus_tag="VEO00_04835"
     CDS             complement(3313..3906)
                     /locus_tag="VEO00_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYU57359.1"
                     /translation="MSNAATSNQEILERFARVVAQSLRIEASLVTEEAYLDDLGAESL
                     DLAEITMESEEEFDVLIAQKTILQTATEVFGEGVLVHDGKLTEEGKRLLRRRMPDFPG
                     DKDEITVADLGKLFLRVGTWVRMIRGLTEHTPRDCAKCGAAYGKPLAGRLKCRSCGFE
                     HDIPSGEDLNRQWVQQYYQREYVPSRPLAASPPAPVA"
     gene            complement(3951..5225)
                     /locus_tag="VEO00_04840"
     CDS             complement(3951..5225)
                     /locus_tag="VEO00_04840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HYU57360.1"
                     /translation="MSKHRVVVTGLGIVTALGTGVPVVWRRLLAGENGIGPISYFDAS
                     QYSCRVAAEVRNLPLNDSGLQSVPLGYCRRGARLFLHAARGAHADAGLTGAGLAPHRI
                     GVAAGTTVNYVNMRLVRHYFRFRRAQSPHIDLARFAREGEQPPALFYRRQGEMTSVLA
                     AKALGLGGPNLAIDTACAASAYAVGEAFRMVQRGQVDAMMAGGACSIVSPVAILAFAI
                     LGALSANPDPDEASRPFDRRRDGFVMGEGGGAVVLERLEHARARGAGIYGEIAGYGTT
                     MNAANLTDPSPDGTSEERAMRLALQEASLAPEEIDYVAAHGTSTPKNDAVETAALKRV
                     FGAHARRLMVSSNKGQIGHTISAAGVCNLICALKAIEEGWVPPTAHYRDPDPQCDLDY
                     VPNVGRQAGVRAALVNAFAFGGQNAVLAVKAA"
BASE COUNT          824 a         2123 c         2076 g          802 t
ORIGIN      
        1 ttcatcatgc ccgtcacgcc cgccgccgac ttggtgtgcc cgaccatgga tttgacggag
       61 ccgatggcgc agctcgaagg agcggcgccc gcggcctgga acacctccga gagcgcggcg
      121 acctcggcgg cgtcaccgac gaccgtgccc gtgccgtggg cctcgacgag gccgaccgtg
      181 gccggagaga tgccggcccg cgcgtacgcg cgctcgaggg ccagggtctg gccttccggc
      241 cggggcgccg tgaggctgcg gccccgcccg tcgctcgagc ccgccaccgc cttgaccacg
      301 gcgtagatgc ggtcgccgtc gcgctcggcg tcggccagcc gcttcagcac gagcatggtg
      361 atgccttcgc tgatggcgat cccgtccgcg ccggcgtcga aggtgcggca acgtccgcgg
      421 ggggagagcg cctgcgattt gctgaagcag aggaagccga acgggctctg caccgtgtcg
      481 accccgccca cgaccaccat gtcgctggtg cccgcctcca gctcggacac cgccaggtag
      541 gcggcggcca gcgacgacgc acaggcggcg tccaccgtgt agttcacgcc gccgaggtcg
      601 aagcggttgg ccacccggcc cgcggccacg ttgagcagga tccccgcgaa agagtcctcc
      661 gtccactccg gcagccgctc gagcacctgg gccggcgctc ggccgaggac cgccggcagg
      721 ttcgagcgga tcccgtagcc gaggccgagg tcgccgagcc cgccgctcac gccgaggatc
      781 accgaggtgc gcgcacgcgg gaagtcgcgg tcgagatatc cggcgtcggc gagggcctgg
      841 cgcgccacct ccagggagag caactgcaag gagtcgatcg aggggagcgc ggccggcggg
      901 atgccgtacc gcatggggtc gaagggcacg tcgtcgagga acccgcccca gcgcgagtag
      961 accttgtcgc gcgccttgcg gtcgggatcg aagtacaggt cgatgtcgaa ccgctccttc
     1021 ggcacctcgg tgatcgcgtc gaccttgttc aggacgttcg cccagaaggt cggcacgtcc
     1081 ggcgccttgg gcagcagaca ccccaagccg atgatcgcga cattgcaggg gcgcgccgcc
     1141 tcggggacgg ctctcggcgc aggcccggcc ggcagggcca ggccggcgag caggcgcgcg
     1201 ccccccctcg acacgccctc gtgcagatcg gcgatgcggc acacgccctt ccggagcgcg
     1261 gcgagctggc cgatcatgaa cattccgtcc cggtgctgcc gctcggggtc gacctcgacg
     1321 tatcggggcc cgctcccctc cgcctcccca tcgcgggcga tacccttgga cgcgatgcgg
     1381 agccgcccca ggttgagggc ctccagctcg cggcggagct cctccggcga cttgccttcc
     1441 gccagcagcc ggcgcttggc ctcgcggaac gtctcgtaga agggcgtgtc gacgcagcgc
     1501 gtcgagtggc ccgggcccgt ctcgagcagc accgtatggg cgcagcccag ggcttcgcgc
     1561 tgaaatccct cgacgatggc gccggcggcg acggcttcct cggtgaagag gtagcccgtg
     1621 ccgaggagca ccccgatgcg tgccccccgc tcgacgaggg gcgccgccat ggcggcgacc
     1681 atggccgccg agcggtcgtc atggacaccg cccgcgaaca cgatgtggag gtggtcggcg
     1741 ggaatcccgg cctcgagggc gtcgagcagc gcgtcgatca tggtctgcca cagcacgaag
     1801 ctcgtgcgcg gccccacgtg cccgccgcac tcgcggccct cgaagatgaa gcggcgcgcc
     1861 ccgtcttcca cgaacatccg caggaggccg ggcgcgggga cgtgcaggta ggtggcgatg
     1921 ccgttggcct cgagccgcgc cgcctggtcg ggccgcccgc ccgcgatgat cgcgaagggc
     1981 ggccggaagt cgtggatcac ctcgagctgc tcctggcgca gctcgagggg tacgaacccc
     2041 aggatcccga cgccccacgg ccggtccccg aggcgctcgc ccgtctcctg gaggagcgcc
     2101 cgcacctggg ggccgcgcag cagcgcgagg gccaggaacg gcagggcgcc gccccgcgcc
     2161 accgcttccg cgaacgccgc ggtgtcgctc acgcgggtca tcggaccctg caggatcggg
     2221 tactcggtgc cgtgcgaccg ggcgaggggg gcgccgcggt ccagcgcgcg ctggccgcgc
     2281 gcgagcctca cgtgatcaac gacggaggcg cgcaggccgt cgaggatgcc ggcgacggtg
     2341 tggaagcgcc gggcgagccg cgcggcgaag gccccgtcct gccccagggg ccagagctgc
     2401 gcttccgggc gttcccagcc gacccgcgcc tggatcgcct cccgccagcg gtcgcgggcc
     2461 gccccggcgg gctgggccgc gagcgtggcc tccacctgcg ggagcgcgtc cagggcggcc
     2521 atgcccggcc gctgatagac ccggtagagg tccccgatct ccgcgccgac gcagagcgtc
     2581 tcgtcgccct ccatgcgctc gagggccgcg cgcagccttg gcggcagcgc cgactcccgc
     2641 gccagggcga gctgcgcgtc gacgacgacg ccgctgcatc cggccacgta ggcggcggga
     2701 gcggagtgca ggccgatgcc gccgtaggcg aggacggggc ggtcggtctc ccgcaacagt
     2761 cgctggagga gtatgaacgt cgtctcttcg ccgacgtgcc ccccggcctc gtggcccttt
     2821 gcgaccagac cgtcggcgcc gctctccagc gccgcccggg cctcttcgac gctggtgcac
     2881 tccaacagga cccggcggcc gggcgcgcgc acgagcgcga tcgcggatcg ggccgagagg
     2941 gcgtcggccc cggcgacgac cacgaggttg atcgtcggcg gcagggaggc cgcgaggtcg
     3001 ccgctcgtcc ccctcccggg ccggaccttc acgccgagcg agccccggcc gaaggagccg
     3061 agccgggcga gggccgaccg ggccgccgcg aggtcggtcg tgtgctcgag gttgagcagc
     3121 ccggtggctc cggcgcgact cgccgcgatg gcgacggcgg gatcgggcca gccgggcagg
     3181 gtggcgccga cgatttcaaa ggcgcggtcg gcttctgggt tcgtcacggc aaccatgctt
     3241 cctcgggccc ggaggtggct cggagccacc gccgcccgga gcatcgaccc ggaccgaacg
     3301 gggacgatcg cgtcacgcca ccggcgccgg aggcgacgcc gcgagcggcc gcgaggggac
     3361 gtactcccgc tggtagtact gctgcaccca ttgccggttc aggtcctcgc cggagggaat
     3421 gtcgtgctcg aagccgcagg aacgacactt gagacggccg gccaacggct tgccgtaggc
     3481 ggcgccgcac ttcgcacagt ccctgggcgt gtgctccgtc agccccctga tcatccgcac
     3541 ccacgtgccg acccgcagga acagcttgcc caggtcggcc accgtgatct cgtccttgtc
     3601 gcccggaaag tccggcatcc gccggcggag aagccgcttg ccttcctcgg tgagcttgcc
     3661 gtcatggacg agcacgccct cgccgaatac ctcggtggcc gtctggagga tggtcttctg
     3721 cgcgatgagg acgtcgaact cctcctcgct ctccatggtg atctcggcca gatccagcga
     3781 ctcggcgccg aggtcgtcga ggtaggcttc ctcggtgacc agggacgcct cgatccggag
     3841 gctctgcgcg accacccgcg cgaacctctc cagaatctcc tggttcgacg ttgccgcatt
     3901 cgacatcgtt tctctcctgc tccttctgaa gcgagcccga cccgtcccgc ctacgccgcc
     3961 ttcaccgcca gcacggcgtt ctgtcccccg aacgcgaacg cgttcaccag cgccgcccgg
     4021 accccggcct gccggccgac gttgggcacg tagtcgaggt cgcactgcgg atcagggtcg
     4081 cggtagtggg cggtcggagg cacccacccc tcttcgatcg ccttcagggc gcagatcagg
     4141 ttgcagacgc ccgccgccga gatggtgtgc ccgatctggc ccttgttcga cgacaccatc
     4201 agccgccggg catgggcccc gaacacgcgc ttcagggccg ccgtctccac cgcgtcgttc
     4261 ttgggggtcg aggtgccgtg ggcggccacg tagtcgatct cttcgggggc gagcgaggct
     4321 tcctgcagag ccaggcgcat cgcccgctcc tcgctggtcc cgtccggcga cgggtcggtg
     4381 aggttcgcgg cgttcatggt cgtgccgtag ccggcgatct cgccgtagat cccggccccg
     4441 cgggcccggg cgtgctcgag gcgctcgagc accacggcgc cgcccccctc gcccatcacg
     4501 aagccgtccc ggcggcggtc gaacggccgg gaagcctcgt cgggatccgg gttggcgctc
     4561 agcgccccca ggatcgcgaa ggcgaggatg gccaccgggc tgacgatgga gcaggcgccg
     4621 ccggccatca tcgcgtcgac ctggccgcgc tggaccatgc gaaacgcctc gccgacggcg
     4681 tacgcgctgg cggcgcaggc ggtgtcgatg gcgaggttcg ggccgcccag cccgagggcc
     4741 ttcgcggcga gcacgctggt catctcgccc tggcggcggt agaagagcgc gggaggctgt
     4801 tcgccttcgc gcgcgaagcg ggcgaggtcg atgtgcgggc tctgggcccg gcggaaccgg
     4861 aagtagtggc ggacgaggcg catgttgacg tagttcacgg tggtgccggc ggccacgccg
     4921 atccggtggg gggccagccc ggctccggtc agccccgcgt cggcgtgcgc cccgcgcgcg
     4981 gcgtggagga acagccgcgc gccccgacgg cagtagccga gcggcaccga ctggaggccc
     5041 gagtcattca ggggaaggtt ccggacctcg gccgcgacac ggcaggagta ctgggaggcg
     5101 tcgaagtagc tgatgggccc gatgccgttc tcccccgcga ggagacgacg ccagacgacc
     5161 ggcacaccgg tgcccagcgc ggtgacgatt ccgaggcccg tgaccacgac tcgatgcttc
     5221 gacaccagag cctttctcga cgatgcgggg ctcaccctgg cccggtccgg atcctgcgct
     5281 ctccggcacg ctcagcgcct cccaccacga ggatggtgtc gcgaagaacg cctccctcaa
     5341 cacccacgat ccgcgcccgg agccatgcgc agggccgcgg gtgtccggct ttttttgaac
     5401 acggtctgca agatgtcagc cagatctgcc gatcgctaag gcgttgtgac aggccggcat
     5461 ctgaggcact tgtgccggcc gtgatgacgc taatcgtcgc cggagaagac ttttgtctcc
     5521 cctcctgccg ctcccggccc gccaatacgc gctcttcgga gagaccgcac acctcggccc
     5581 gggagtgcgg ttcggagagg gaagacttca aacatccaag cggtagactt ccacgacgcc
     5641 gccgctcaac ccgtccggct gttctcgacc ggatcgcaac ggagaatctg caaccgccgt
     5701 tgacgccctg cggaccccgc agtacaatcc gccgcgttcg aattcacaca gctgcgtgac
     5761 gttgcccgag gcgcccgatc ctcgacggtc cgatctttca cactggaggc ctgcatgccg
     5821 cggat
//
LOCUS       DASPWO010000218         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_288421, whole genome shotgun sequence.
ACCESSION   DASPWO010000218 DASPWO010000000
VERSION     DASPWO010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288421"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VEO00_04845"
     CDS             complement(<1..258)
                     /locus_tag="VEO00_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57361.1"
                     /translation="MRDVNLFVSRLLAGLMIVSVPAGALGEGTKVGGHVGLATSLVTR
                     TSESSRDTDISDTFTLVAPIGVTVKLSDRLAIDFETQVANRL"
     gene            914..1390
                     /locus_tag="VEO00_04850"
     CDS             914..1390
                     /locus_tag="VEO00_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57362.1"
                     /translation="MGGTSTGARPGEVSGAGPAGEVSASAGAAKEPGHEISGKVDKYD
                     REHRTLSLANSPTTLQLSDDTEVVKDGQKVSPGQIMEGDEVRASYAGSGETVQAQHIE
                     ISSGTPSSTGTGGTSAGPATGTESGSAPGGGSPKGTAAGKGSDVSGGSTGGNAPPR"
     gene            complement(1505..2431)
                     /locus_tag="VEO00_04855"
     CDS             complement(1505..2431)
                     /locus_tag="VEO00_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYU57363.1"
                     /translation="MIRTALLLLACALAACKGYRAPHFDAPQKLGGQVVPAGVLTSGA
                     RAYALYCRSCHGDKGDGKGTAARGLRPPPRDFTLGMFKFGAVPAGSLPHDEDLERIVR
                     TGLHGTAMLAWDGIPQPTVREIIQYVKTFSPRWKDEEPGEAITPTDDPWKDRAAEGVA
                     RGRKVYHGLARCQGCHPAFASKPFIYAATKELSGSGSADFRDDMYGSEVKESEFGVKL
                     LPPDFTRSELRSIREDHRIEDLYRVIASGVGGTAMPTWHGSLPEEEIWALVHYVDSLV
                     ALKGTNAARRLVEENLAADARWTPPQEPAQSR"
BASE COUNT          389 a          889 c          926 g          330 t
ORIGIN      
        1 aaggcggttc gcgacctggg tctcgaagtc gatggcgagc cggtcgctca gcttcacggt
       61 gacgccgatg ggagccacga gggtgaaggt gtcggagatg tccgtgtccc tgctcgactc
      121 cgaggtgcgc gtcaccagcg acgtcgccag gccgacgtga ccgccgacct tcgtgccttc
      181 gcccagggcg ccggcgggga cgcttacgat catcaggccg gccagcagcc ggctcacgaa
      241 cagattcacg tcgcgcatgc tcatataccc tccacgcgag gcgagcgagt cgcctgccgg
      301 gattcacggt aggtactcgg ccgcgcgggg gcgaggtcgt ggaggtgatt caacgctcgg
      361 tcgttgaggc gtcggcgcgc gctgggtgaa cgggcggtcg gtccacgacc ggtgtacgaa
      421 ccgctcgcgc cccgtgcgac tcagacttcg gcggcaccca gcgcgcgtcg gccgcgaagg
      481 cgggatgtga cagttcgggg agggcgggaa ttcccgaaca cgcaccgcgt cggcggcccg
      541 ctcgagaagt aggcgacgag ctcacgtccg cgcgtctccc gctccagcct ggctcggcgc
      601 cgcgcgggtc cctaccctcc tgaggaaggc cgactggcga ccacggccat ggggtggggc
      661 tccgccgtcg gcgggagcga tgcagatgaa caggatcgca tggagtgcag tgaccgggat
      721 cggaatcgcg ggcgcgctgc tcgcgacgcc actggtccgg ggggctgccg aggaaacgaa
      781 gggacactcg acctcggcag acgagacgcg gcaaggtggc accgccaccg gaggcgcctc
      841 gacagcgccg caggaccagc ccggcgcgaa gagcggcacg accccgtcga gcgagggcgc
      901 gaggggcagt gggatgggcg gcacgtcgac cggagcacgg cctggcgagg tcagtggcgc
      961 cggccccgct ggagaggtct cggcctccgc gggagccgcg aaggagccag ggcacgagat
     1021 cagcggcaag gtcgacaagt acgaccggga gcaccggacg ctctcgctcg cgaactcccc
     1081 cacgacgctg cagctctcgg acgatacgga ggtcgtgaag gacggccaga aggtctcccc
     1141 cgggcagatc atggagggag acgaggtccg ggcgagctac gcggggtcgg gcgaaacggt
     1201 ccaggcgcag cacatcgaga ttagttcggg gaccccgagc tccaccggaa ccggtgggac
     1261 gagcgccggt ccggcgaccg ggactgagtc cgggtccgcc cccggtggcg gctctcccaa
     1321 gggcaccgcc gcgggcaagg gaagcgacgt gagcggcggc agcaccggcg gcaacgcgcc
     1381 gcctcgctga cgcgctgagc gacggggccc tgccggcctg ccgctgcgct cgacggtccg
     1441 gccccgtccc tcgctcgaat gccctggcgg gacggcgatg acgcctcgtg ccgtccccac
     1501 cgtcctaccg cgactgcgcg ggctcctgcg gaggggtcca gcgcgcgtcg gccgcgaggt
     1561 tctcctcgac gagccgtcgc gcggcgttgg tccccttcag ggcgacgaga gagtccacgt
     1621 agtggacgag tgcccagatc tcctcctccg ggagagaccc gtgccacgtc ggcatcgcgg
     1681 tcccgccgac gcccgaggcg atcacgcggt acaggtcctc gatccggtgg tcctcccgga
     1741 tcgagcgcag ctccgatcgc gtgaagtccg gcggcagcag cttcaccccg aactcgctct
     1801 ccttgacctc ggagccgtac atgtcgtccc ggaagtcggc ggacccggac ccggacagct
     1861 ccttcgtcgc cgcgtagatg aacggcttcg acgcgaacgc ggggtggcag ccctgacagc
     1921 gggccaggcc gtggtagacc ttccggccgc gggcgacccc ctcggccgcg cggtccttcc
     1981 acggatcgtc ggtgggggtg atcgcctcgc ccggctcctc gtccttccag cgcggcgaga
     2041 acgtcttcac gtactggatg atctcccgga cggtgggctg cggaatgcca tcccacgcca
     2101 gcatggcggt gccgtgcagg ccggtccgca cgatccgctc gaggtcctcg tcgtgcggca
     2161 gcgagccggc gggcactgcc ccgaacttga acatcccgag ggtgaagtcg cgcggcggcg
     2221 ggcgcagccc cctcgccgcc gtgcccttcc cgtcaccctt gtcgccgtgg cacgagcggc
     2281 agtagagcgc gtatgcccgt gcgccgctgg tgagcacccc ggcgggcacg acctggcccc
     2341 cgagcttctg tggcgcgtcg aagtgcggcg cgcggtaccc cttgcacgcg gccagggcgc
     2401 aggcgaggag caggagcgcg gttcggatca tgacgacctc ggatcacacg aggtagacgg
     2461 ccacgtacag cgcggcccac accaccccga cgaagtgcca gtaccatccc cacagccgca
     2521 cctccgtccg gcgg
//
LOCUS       DASPWO010000219         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1217484, whole genome shotgun sequence.
ACCESSION   DASPWO010000219 DASPWO010000000
VERSION     DASPWO010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217484"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VEO00_04860"
     CDS             complement(<1..596)
                     /locus_tag="VEO00_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HYU57364.1"
                     /translation="MATIPAELGKRLRKRITARATEADLDAFDPEFIRDNLPAMWLLA
                     TLYHRAEVRGFERVPAAPTLLVGNHNGGIVIPDTWITGLGWYAYFGVERPMRSLVHSA
                     VMSLPIVGDAMRKLGSMPASPENARLGLRRGEDVLVYPGGDVETWRPWSKRNEIDFDG
                     RTGFLRMAAEEGVPIVPVVSIGAHETLVVLTDGKDLAKR"
     gene            697..1431
                     /locus_tag="VEO00_04865"
     CDS             697..1431
                     /locus_tag="VEO00_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU57365.1"
                     /translation="MSKPRVLVVEDDPAIAEPLGESLAREGFLPHLAASAAEALSAFG
                     SEKFDAVLLDVMLPDGDGRDVARQIRSGSDVSIIMVTARGEEVDRVLGLELGADDYVV
                     KPFSAKELAARIRAVLRRGRSVAERVESIEVGEVRLDPASRAVTLAGDAVELAAREFD
                     LLHYLMRHAGAVVRREDIMDDVWDPHWFGSTKTLDVHVSWLRKKLETDPAKPQYLTTV
                     RGVGFRFAAPQELPGGGRRGRRGRSR"
     gene            1439..>2029
                     /locus_tag="VEO00_04870"
     CDS             1439..>2029
                     /locus_tag="VEO00_04870"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57366.1"
                     /translation="MRVRTRLLLAFAYILIVVIVALEVPLAFNLNRRALAELEARALV
                     QAQAMAGAVEEQELSPANAGVLQRLAAQVAEPGVTRVIVTDAQGALLADSDSPGTTGA
                     SYENRPEIQRVLAGEPFVQVTRFSTDLGQDILATAVPVTRSNRLAGAVRITQSLTGVR
                     AEVRRIVLGLAAVGLVGLAAGLAVAWVLAGTLSRPLT"
BASE COUNT          260 a          728 c          748 g          293 t
ORIGIN      
        1 cgcttggcca ggtccttgcc gtcggtcagc acgacgagcg tctcgtgcgc gccgatcgac
       61 acgaccggga cgatcgggac gccctcctcg gcggccatcc gcaggaaccc ggtccgcccg
      121 tcgaagtcga tctcgttgcg cttcgaccac ggacgccacg tctccacgtc gccgcccggg
      181 tacacaagga cgtcctcgcc gcggcgaagc ccgagccggg cgttctcggg cgacgccggc
      241 atcgaaccga gcttgcgcat cgcgtcgccg acgatcggca gcgacatcac cgccgagtgc
      301 accagcgagc gcatcggccg ctcgacgccg aagtacgcgt accagcccag gccggtgatc
      361 cacgtgtcgg ggatgacgat cccgccgttg tggttgccga ccaggagcgt cggggcggcg
      421 gggacccgct cgaacccccg cacctcggcc cggtggtaga gggtggcgag cagccacatc
      481 gccgggaggt tgtcccggat gaactccggg tcgaacgcgt cgaggtccgc ctcggtcgcg
      541 cgggccgtga tgcgcttccg gagccgcttg ccgagctccg ccggaatcgt cgccaccgct
      601 ccaagtctgg ccccacgccc ggccctcgca caacccggcc gggctgcgcc gatcctttac
      661 cggaccttgg cctgcgcttc gctacgatcg gggcgcatgt cgaagccgcg cgtgctggtc
      721 gtggaggacg acccggcgat cgccgagccg ctcggcgaga gcctggcgcg cgaggggttc
      781 cttccccacc tggccgcgag cgccgccgag gccctctcgg cattcgggag cgagaagttc
      841 gacgcggtgc tgctcgacgt gatgctcccc gacggcgacg ggcgcgacgt cgcgcgccag
      901 atccgttccg gatctgacgt gtcgatcatc atggtcaccg cgcgtggcga ggaggttgac
      961 cgcgtgctcg gcctggagct cggcgccgac gactacgtgg tcaagccgtt ctccgcgaag
     1021 gagctcgcgg cgcggatccg cgccgtcctg cgccgggggc gctcggtcgc cgagcgcgtc
     1081 gagtcgatcg aggtcgggga ggtgcggctc gacccggcct cccgcgcggt gacgctggcc
     1141 ggcgacgcgg tcgagctcgc ggcgcgggag ttcgacctgc tgcactacct gatgcgtcac
     1201 gccggggcgg tggtgcggcg cgaggacatc atggacgatg tctgggaccc gcactggttc
     1261 ggctcgacga agacgctcga cgtgcacgtg tcctggctgc gcaagaagct cgagaccgac
     1321 ccggcgaaac cgcagtacct gaccacggtg cgcggggtgg ggttccgctt cgccgccccg
     1381 caggagctcc ccgggggcgg tcggcgcggt cggcggggcc gctcccggta ggagatccgt
     1441 gcgcgtccgc acgcggctgc tgctcgcgtt cgcctacatc ctgatcgtag tcatcgtcgc
     1501 gctcgaggtg ccgctggcgt tcaacctgaa ccgccgggcg ctcgccgagc tggaggcgcg
     1561 cgcgctggtc caggcccagg ctatggccgg cgcggtcgaa gagcaggagc tctcccctgc
     1621 gaacgctggg gtcctgcagc ggctggccgc ccaggtggcc gagccggggg tcacccgggt
     1681 gatcgtcacc gacgcgcagg gggcgttgct ggccgacagc gattcgccgg gtaccaccgg
     1741 cgcctcctac gagaatcggc ccgagatcca gcgcgtcctg gccggcgagc cattcgttca
     1801 ggtcacccgg ttcagcaccg acctgggcca ggacatcctg gccaccgccg tcccggtgac
     1861 gcgcagcaac cgcctggccg gggccgtccg gatcacgcag agcctgacgg gcgttcgcgc
     1921 cgaggtgcgg cggatcgtgc tcgggctggc ggccgtcggg ctggtcggcc tggccgcggg
     1981 gctggccgtg gcgtgggtgc tggcggggac cctctcccgg ccgctaacg
//
LOCUS       DASPWO010000220         7131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_114208, whole genome shotgun sequence.
ACCESSION   DASPWO010000220 DASPWO010000000
VERSION     DASPWO010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114208"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2650)
                     /gene="cphA"
                     /locus_tag="VEO00_04875"
     CDS             complement(<1..2650)
                     /gene="cphA"
                     /locus_tag="VEO00_04875"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HYU57367.1"
                     /translation="MRVYRGPNYWSYEPAVRMLVDLGSLEAFPSNTIPGFNEALTELL
                     PGLADHHCSLGRRGGFLGRLRDGTWAGHVAEHVALELQRETGAEIRRGKTRSADEPGR
                     YNVIYGYQEEAVAAEAGKLAVRLVNHLVRAERGFDYRASLEALIRLAQRSAFGPSTQA
                     LVDEALARDIPYIRLNEYSLVQLGQGVHQKRIRATMTSTTPSIAVDIASDKKLTNRLL
                     AAAGLPVPRSVVVTGEDEAVAAARRIGFPLVTKPLDGNHGRGVSLDIRDLEGVRKGFA
                     LARSETRSGRVVVESYVDGNDYRVLVVGGNMVAVAQRVPANVTGDGTRTVADLVEEAN
                     ADPRRGIGHEKVLTRITVDAESLEIVRHQGFGLDDVPPKGEMVKLKLTGNMSTGGISI
                     DRTDEAHPDNVEIAEEAARVVGLDVAGIDFIAPDISRPVRETGGAIVEVNAAPGFRMH
                     THPTEGEPQYVAKPVIDLLFPPGSASRIPIVAVTGSNGKTTTTRIIAHVFKGMGRKVG
                     MTSTDGIYIDERLVKKADASGPKSAQMVLQNPRVDFAVFEVARGGILREGLGYGRNDV
                     AVVMNVTGDHMGLGEVKTLRQLAAVKRVIVEAVPRDGQAVLNADDPLVAEMRKHCSGS
                     IVFFTMKPPSELVERHVRRGGAAVVLGEGTHGEMIVIRHGRRAMPLAYTHLLPATFGG
                     KARMNVQNALAAAGAAYAAGAHLHDIRQGLRTFSTSYYQAPGRLNLFELDRVRVLVDY
                     AHNPAAMQALGDFVDHMTTGGDGWQANLRVGVIATPGDRRDQDMRQVGRIAARYFDEI
                     IVREDHNPRGRQRGETAALVAEGIRQSMRKGARCGAVDVIVDEMEATRAALDRSRPGD
                     LVVLCIDYATEVWRELQARLEASTEAV"
     gene            complement(2761..3717)
                     /locus_tag="VEO00_04880"
     CDS             complement(2761..3717)
                     /locus_tag="VEO00_04880"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HYU57368.1"
                     /translation="MASRASDPGAVLAIGGAEDKLKDRVILGRFIQLAGGEGARIAVV
                     STASSLGDEATELYRALFRQAGAGEVAGMRPTTRAEANDPSLARMVRAASGVFLTGGN
                     QLRLSSVVAGTLLGRELLAAHRRGAVIAGTSAGSSALSTHMVAFGASGATPKHRMVQV
                     ASGLGIVTNVIIDQHFEQRNRLGRLLSAIAQSPNLLGVGLDEDTAALFEGDELEALGK
                     GAVTIVDGSDIVTDAFFTKGHRPMMVSGAVIHSLPGGWRYDLVERRLVSRDVGTVSPD
                     AQRWAGQRVRRQVRELAAEGADDRAPIRAARRRAQVKEEASE"
     gene            3820..4788
                     /locus_tag="VEO00_04885"
     CDS             3820..4788
                     /locus_tag="VEO00_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYU57369.1"
                     /translation="MRTDTAPRAFVPPPRWLDLDGRTHYVRWDGPELGIPWVLVHGLG
                     GSLLNWMAVGPALAERGPVYALDLAGFGRTPLDDRSATPHANRLLVHRFIHRVVGRPV
                     LLAGNSMGGSIAMLEAAVGDRTVAGLILTDPALPLVRRTPSDPRVVALFAAQFIPGFG
                     EWFARSRYRRLGPERLMDETLRLCTQDPSRIAPEVREAHVALARERAQMGHAVPAFLR
                     ATRSVVYATLFPAAPWRAARAVPAPALVVHGAADRLVPLAAAHRLHRLRPDFHLTVID
                     DVGHIPQLEAPERWLEAVESWLADRDVASEVERRAAAPLRPPRIAG"
     gene            4803..5840
                     /gene="speB"
                     /locus_tag="VEO00_04890"
     CDS             4803..5840
                     /gene="speB"
                     /locus_tag="VEO00_04890"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HYU57370.1"
                     /translation="MGDKRPSPGVRVNRAHEPSYAGGPTFSKLRLVLEPSGLAGVDVA
                     IVGAPTDETTSYRPGARFGPRAIRTADYGGGSPPSDHHMDLGLDPYTVLSVVDYGDVP
                     VRPADALYNRDRIRETVGEIVAAGAVPVVLGGDHAIAYPDVSAVAEALRPATVGIVHF
                     DAHADDAEDVWEVRYSHGTPLRYLVEEGWVRGEHVVQIGLRGYWPFPEEFAWARERGL
                     RWFRMDVVEDRGIGPVIEETLEMLARCDHLFLSVDIDVVDPSAAPGTGTPEPGGMTAR
                     QLLRAVRRLAAARGFAGMEVVEVSPPYDVAEITARLAHRVVLEALSGLALHRTGRAPA
                     PEDPTPSAASG"
     gene            complement(5815..6915)
                     /locus_tag="VEO00_04895"
     CDS             complement(5815..6915)
                     /locus_tag="VEO00_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYU57371.1"
                     /translation="MVHALPFSIGEPGPLMSALLPSIEEAIRLGLEETRAVVAAAAPE
                     ATLLADELERAVLAGGKRLRPVLCVLAHGAAGGTAGTEILQAAAALELLHAFALVQDD
                     VMDRAETRRGATALHRRLAASPALPPGAGESAGVVAALLVADLALLAADRLLDECGFP
                     PERILAARGEYDRARLDAVAGQFLDVVLPADPSLDEAGAERIGALKSGRYSVEGPVLV
                     GVALAPGGGPARAALARYAAALGRAFQIRDDVLGAFAGPELTGKDGGADLRHGKRTVV
                     LTKTLLRATVAERRFLEARVGTSGLTDGEVEQVREVMSDSGALAETLTAIDALIAEAV
                     AALDGAPLAPGAAAGLAELAEGLRLTPTRPTG"
BASE COUNT          881 a         2752 c         2512 g          986 t
ORIGIN      
        1 cgacggcctc ggtggaggcc tccaggcgcg cctgcagctc gcgccacacc tcggtggcgt
       61 agtcgatgca gagcacgacc aggtcgcccg ggcggctgcg gtcgagcgcg gcgcgggtgg
      121 cttccatctc gtcgacgatg acgtccaccg ccccgcagcg ggcgcccttg cgcatcgact
      181 ggcggatgcc ctcggccacc agcgccgccg tctccccacg ctggcgcccg cgcgggttgt
      241 ggtcctcgcg gacgatgatc tcgtcgaagt agcgggcggc gatccggccc acctggcgca
      301 tgtcctggtc gcgccggtcc cccggggtgg cgatcacccc gacgcgcagg ttcgcctgcc
      361 agccgtcgcc gccggtggtc atgtgatcca cgaagtcgcc gagcgcctgc atcgccgcgg
      421 ggttgtgcgc gtagtccacc agcaccctca cccgatcgag ctcgaacagg ttcaggcggc
      481 cgggagcctg gtagtaggac gtcgagaacg tgcgcaggcc ctgccggatg tcgtgcaggt
      541 gcgcgccggc cgcataggcc gcgccggccg cggccagcgc gttctggacg ttcatgcgcg
      601 ccttcccgcc gaacgtggcc ggcaggaggt gcgtgtaggc gagcggcatc gcccggcggc
      661 cgtggcggat cacgatcatc tcgccgtgcg tgccctcgcc gagcaccacg gccgcgccgc
      721 cgcggcggac gtgtcgctcc accagctcgc tcggcggctt catcgtgaag aacacgatcg
      781 agccggagca atgcttgcgc atctcggcca ccaacgggtc gtcggcgttc aggacggcct
      841 ggccgtcgcg gggcaccgcc tcgacgatca cccgcttcac cgccgccagc tgccggagcg
      901 tcttcacctc cccgagcccc atgtgatcgc cggtgacgtt catcaccacc gccacgtcgt
      961 tgcggccgta gccgaggccc tcgcgcagga tcccgccacg ggcgacctcg aacaccgcga
     1021 aatccacgcg ggggttctgc agcaccatct gcgccgactt cgggcccgag gcgtcggcct
     1081 tcttcaccag ccgctcgtcg atgtagatgc cgtcggtcga ggtcatcccg accttccgcc
     1141 ccatcccctt gaatacgtgc gcgatgatcc gcgtcgtcgt cgtcttcccg ttcgagccgg
     1201 ttaccgcgac gatcgggatg cgcgacgccg agcccggcgg aaagagcaga tcgatcaccg
     1261 gcttggccac gtactgcggc tcgccctcgg ttggatgggt gtgcatccga aagcccggtg
     1321 ccgcgttcac ctcgacgatc gcgccgccgg tctcgcgcac cggacgcgag atgtccgggg
     1381 cgatgaagtc gatcccggcg acgtcgaggc caaccacccg cgcggcctct tcggcgatct
     1441 cgacgttgtc ggggtgtgcc tcgtcggtcc ggtcgatcga gatgccgccg gtggacatgt
     1501 tcccggtcag tttcagcttg accatctcgc ccttcggcgg gacgtcgtcc agcccgaagc
     1561 cctggtggcg gacgatctcc agggactcgg cgtcgaccgt gatccgggtc aggaccttct
     1621 cgtggccgat cccgcgccgc gggtcggcgt tcgcctcctc cacgaggtcg gcgacggtcc
     1681 gcgtgccgtc gccggtcacg ttggccggga cgcgctgggc gacggcgacc atgttcccgc
     1741 cgacgaccag cacccggtag tcgttgccgt ccacgtagga ctcgaccacc acccggccgc
     1801 ttcgcgtctc cgagcgcgcc agggcgaacc ccttccggac tccctccagg tcgcggatgt
     1861 cgagcgacac gccgcggccg tggttcccgt ccagcggctt cgtcaccagc gggaacccga
     1921 tccgccgcgc ggccgccacc gcctcgtcct cgcccgtgac caccaccgac cgtgggaccg
     1981 gaagcccggc cgccgccagg aggcggtttg tcagcttctt gtcgctcgcg atgtcgaccg
     2041 cgatcgaggg cgtcgtcgag gtcatcgtgg cccggatgcg cttctggtgg acaccctgtc
     2101 cgagctgcac gagcgagtac tcgttcagcc ggatgtaggg gatgtcgcgc gccagggcct
     2161 cgtccaccag ggcctgcgtg gaggggccga acgccgagcg ctgcgccagc cggatcagcg
     2221 cttccagcga cgcgcggtag tcgaatcccc gctcggcgcg gaccaggtgg ttgaccagcc
     2281 ggacggcgag cttcccggcc tccgccgcga cggcctcctc ctgatagccg tagatgacgt
     2341 tgtagcggcc cggctcgtcc gcgctgcgcg tcttgccgcg ccggatctcg gcgccggtct
     2401 cgcgctgcag ctccagcgcg acgtgctcgg cgacgtggcc ggcccaggtg ccgtcgcgca
     2461 ggcgccccag gaacccgcct cggcgcccaa gcgagcagtg gtggtcggcg aggcccggaa
     2521 gcagctcggt cagggcctcg ttgaagccag ggatcgtgtt cgacgggaag gcctcgagcg
     2581 agccgaggtc gacgagcatg cgcaccgccg gctcgtagga ccagtagttc gggccgcggt
     2641 agacgcgcat ctccaggatg cgcagatcgg gcgccggacg cgccttcgtc tgcttgggcg
     2701 gcgcggggcg cgccttcgtc cgcttcgagg gcgccccggc caccgacgcc cggggcgggc
     2761 tcactccgag gcctcctcct tcacctgcgc ccggcggcga gccgcgcgga tcggggcccg
     2821 gtcgtcggcg ccctcggcgg cgagctcccg gacctggcgc cggacccgct gccccgccca
     2881 gcgctgcgcg tcgggagaga ccgtccccac gtcgcgcgac accaggcggc gctccaccag
     2941 gtcatatcgc catcctcccg ggagcgaatg gatcaccgcg ccggacacca tcatcggccg
     3001 gtggcccttg gtgaagaacg catcggtcac gatgtccgag ccgtcgacga tcgtcacggc
     3061 ccccttgccg agcgcctcga gctcgtcgcc ctcgaacagc gcagcggtgt cctcgtcaag
     3121 gccgacgccc agcaggttcg gcgactgcgc gatcgccgag agcagccgcc cgaggcggtt
     3181 ccgttgctcg aagtgctggt cgatgatcac gttcgtgacg atgcccaggc ccgaggccac
     3241 ctggaccatg cggtgcttcg gcgtggcgcc cgaggccccg aaggccacca tgtgcgtgct
     3301 gagcgcgctc gatcccgccg aggtgccggc gatcacggcg ccgcggcggt gcgcggcgag
     3361 gagctcccgt ccgagcagcg tgccggccac cacgctcgac agccgcagct ggttgccgcc
     3421 ggtcaggaag acgcccgagg cggcccgcac catccgggcg agcgaggggt cgttcgcctc
     3481 cgcgcgcgtg gtgggccgca tcccggccac ctcgccggcc cccgcctgcc ggaacagcgc
     3541 ccggtagagc tccgtggcct cgtcgccgag cgacgaggcg gtgctgacga ccgcgatccg
     3601 ggcgccctcg cccccggcga gctggatgaa cctgccgagg atcacccgat ccttgagctt
     3661 gtcctccgcc ccgccgatgg ccagaaccgc acccggatcg gaagcgcgag aggccatgtt
     3721 cggcaagtgt agccgaccgt tcccaccccc ccgttctcat cggggggttc gctcgcccca
     3781 acccctggcg ccgcgggaac gcggtgtacg ctcgcggccg tgcgcaccga caccgcgccc
     3841 cgcgcgttcg tcccgccgcc gcgatggctg gacctcgacg gccggacgca ctacgtccgg
     3901 tgggacgggc ctgaactcgg catcccctgg gtgctcgtcc acggactggg cgggtcgctc
     3961 ttgaactgga tggccgtcgg accggcgctc gccgagcggg gaccggtgta cgcgctcgac
     4021 ctggccgggt tcgggcgcac gccgctcgac gaccgctcgg ccaccccgca cgccaaccgg
     4081 ctcctcgtcc accggttcat ccaccgggtg gtcggccggc cggtgctgct ggccgggaac
     4141 tccatgggcg gctcgatcgc catgctcgag gccgccgtgg gtgaccgcac cgtggccggc
     4201 ctgatcctga ccgaccccgc gctgccactc gtccgccgca ccccctccga cccgcgcgtc
     4261 gtcgccctgt tcgccgcgca gttcatcccc ggcttcggcg agtggttcgc gcggtcccgg
     4321 taccgcaggc tcggcccgga gcgcctgatg gacgagaccc tccgcctgtg cacgcaggac
     4381 ccctctcgga tcgcccccga ggtccgcgag gcccacgtgg cgctggcgcg ggaacgggcg
     4441 cagatggggc acgccgtgcc ggccttcctg cgggcgacgc gctccgtcgt ctacgcgacc
     4501 ctcttcccgg ccgcgccctg gcgcgcggcg cgggcggtgc cggccccggc gctcgtcgtc
     4561 cacggcgccg cggatcgact ggtccccctg gcggcggccc accggctgca ccgcctgcgc
     4621 cccgacttcc acctgacggt catcgacgac gtcgggcaca tcccccagct cgaggcgccg
     4681 gagcgctggc tcgaggccgt cgagagctgg ctggcggacc gcgacgtcgc ctccgaggtc
     4741 gagcgccgcg ccgcggcgcc gctgcgcccg ccgcggattg ccgggtagcc gctcgatgct
     4801 cgatgggtga caagcgcccc tcgccgggtg tgcgagtgaa ccgggcgcac gagccctcgt
     4861 acgccggcgg gccgacgttc tcgaagctcc ggctcgtcct ggagccgtcc ggcctggcgg
     4921 gggtggacgt ggcgatcgtc ggcgcgccga ccgacgagac gacgagctac cggcccgggg
     4981 cgcggttcgg cccgcgggcg atccgcaccg ccgactacgg cggtgggtcg cctccgtcgg
     5041 accaccacat ggacctcggc ctcgacccgt acaccgtcct gtcggtcgtg gattacgggg
     5101 acgtcccggt ccgcccggcg gacgcgctct acaaccgcga ccgcatccgt gagaccgtcg
     5161 gcgagatcgt cgccgccggc gccgtgccgg tggtcctcgg cggcgaccac gccatcgcct
     5221 acccggacgt ctcggcggtg gcggaggccc tccgtccggc gaccgtgggc atcgtccatt
     5281 tcgacgcgca cgccgacgac gccgaggacg tctgggaggt gcgctactcg cacggcacgc
     5341 cgctgcgcta cctggtggag gagggctggg tgcgcggcga gcacgtcgtc cagatcggcc
     5401 tgcgcggcta ctggccgttc cccgaggagt tcgcctgggc gcgcgagcgg ggcctgcgct
     5461 ggttccggat ggacgtggtc gaggatcgtg gcatcggccc ggtgatcgag gagacgctcg
     5521 agatgctcgc ccgctgcgac cacctgttcc tgtcggtgga catcgacgtc gtcgatccct
     5581 cggcggctcc cggcaccggg acacccgagc cgggtgggat gaccgcccgc caactgctgc
     5641 gggcggtgcg gcggctggcg gcggcgcgcg gcttcgccgg catggaggtg gtcgaggtct
     5701 ccccgcctta cgacgtcgcc gagatcaccg ctcgcctggc ccaccgggtc gtcctcgagg
     5761 ccctctccgg actggcgctg catcgcaccg gccgcgcccc cgccccggag gaccctaccc
     5821 cgtcggccgc gtcggggtga gccgcaggcc ctcggcgagc tcggcgaggc cggcggcggc
     5881 gcccggcgcg agcggcgccc cgtcgagcgc ggcgaccgcc tcggcgatga gcgcgtcgat
     5941 cgcggtgagc gtctcggcca gggcgcccga gtcgctcatc acctcccgga cctgctcgac
     6001 ctccccgtcg gtcaacccgg aggtgccgac gcgcgcctcc aggaaccgcc gctccgccac
     6061 ggtggcccgg agcagcgtct tcgtcagcac gaccgtccgc ttgccgtggc gcaggtccgc
     6121 ccccccgtcc ttgccggtca gctcgggtcc ggcgaacgcg ccgagcacgt cgtcgcggat
     6181 ctggaacgcg cggccgagcg ccgccgcgta gcgggcaaga gccgcccgcg ccggcccgcc
     6241 gcccggggcc agggccacgc cgaccaggac gggcccctcc accgagtatc ggccgctctt
     6301 caacgctccg atccgctccg cgccggcctc gtccagagac gggtccgcgg gcaggacgac
     6361 atcgaggaac tgcccggcca ccgcatcgag ccgtgcgcgg tcgtactcgc cgcgggcggc
     6421 gaggatccgc tccgggggga acccgcattc gtcgagcaac cggtcggccg ccagcagggc
     6481 caggtcggcc accagcaggg cggcgacgac ccctgccgac tcgccggccc ccggcgggag
     6541 cgccgggctc gccgccagcc ggcggtgcag cgccgtcgcg ccgcgccgtg tctcggcccg
     6601 gtccatcacg tcgtcctgca ccagcgcgaa cgcgtgcagc agctcgagcg ctgccgccgc
     6661 ctgcaggatc tccgtgccgg ccgtccctcc ggccgccccg tgggccagaa cgcagagcac
     6721 cggccgcagg cgcttcccgc cggcgagcac ggcacgctcc agctcgtcgg ccaggagcgt
     6781 ggcctccggc gcggccgccg cgaccaccgc gcgggtctcc tccaggccca ggcggatcgc
     6841 ctcctcgatc gacggaagga gcgccgacat cagcggcccg ggctcgccga tcgagaacgg
     6901 cagcgcgtgc accacggccg cagtgtacgg tccgccgccg cacgcggtgc ggcccgggcg
     6961 tcaccctccg tacaggcgct ggatctcgtg gaacagctcg ccgggctcga acgacgcctc
     7021 gaacttcgcc tggacgattc ccagtgccgt caccgcgaag acccaggtgt cgctggtcag
     7081 accccaccgc ccgaggaccg ggtcgagcac gtcgggggtc ggcgagtcga g
//
LOCUS       DASPWO010000221        12413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1133105, whole genome shotgun sequence.
ACCESSION   DASPWO010000221 DASPWO010000000
VERSION     DASPWO010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133105"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VEO00_04900"
     CDS             <1..606
                     /locus_tag="VEO00_04900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU57372.1"
                     /translation="RRRAADLLRSPPLRAAVVAIGVAQASMVAVMGVTPVVIDEHGGG
                     DLAVATTISFHVAGMFAFSPLIGAALDRWGRKPGLVAGALVSAGGAILASVTDNTTVI
                     ATGVFLVGLGWSGAFLASTTMISDLTTAEERAGALGFTDLLASLAAVAGALAAGFLLE
                     GVGFWVVGVSMAALLVPVLALVLPLRESSPGRWGTGAAAAA"
     gene            611..1840
                     /locus_tag="VEO00_04905"
     CDS             611..1840
                     /locus_tag="VEO00_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HYU57373.1"
                     /translation="MARRVLIVALLLAIAAVALVRLHREPPPRPREPFAFTCPAPAPS
                     PPALAEPAPAVGVNTHLLRDDTRNATRCALLDRLTAAGVTWLRLDLRWSEAQPRDPAS
                     GDRFADSLDRGRLARLGSTVDAARARGLRVLVVVFATPQWASGGPVESAGPPLDAAGY
                     GRFLGFLASRYRGRVDAWQVWNEPNESTFFDADGEASFSAVDAAAYATLLRRAAPAVR
                     RGDPDALVVLGGPSYNNVPWLELIYAQGVRGLFDVMAVQPYSIPSCRAPEVDDGSIYV
                     LPAVSRVRALMERVGDGALPIWFTEFGWSSHANPPLAPDYRLGVPEQAQGDYLVRTLV
                     LLRERYPYVEAAFWYRDRDAVIDGQDPTEAAHLGGFGLLRRDLAAKPAYRTLRAYLLT
                     GTVPGRRDAPCPDAAGA"
     gene            complement(1841..2878)
                     /locus_tag="VEO00_04910"
     CDS             complement(1841..2878)
                     /locus_tag="VEO00_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYU57374.1"
                     /translation="MRAARFHAANEPLRLEEVPDPVPGAGEAVVRVEACGICASDLHF
                     ILGEMPLPAPAPVTMGHEAAGVVEALGPGVEGWAPADRVALTAGKGCGACHACREGRW
                     EDCEDPRVLGVHYDGAWAERVLVPAYSLTALPDAVPFEHGAIAADAIATVYSAIVDRA
                     AVRPGDRVGLWGIGGLGTHAVQLARLAGAGFVAAVDPLPAARERALALGADVALDPSE
                     DVPAAIRAAAGGRGLDAAFDLIGRAAVVKQALRSLARGGRAVVVGQSLEPIEAGPVVL
                     YSFRALALIGNYGYRKRHLEAVLDLIATGRLDLSGSVSERLPLARVNEGVERLASKAG
                     DPVRLVVTPTA"
     gene            complement(2891..3697)
                     /locus_tag="VEO00_04915"
     CDS             complement(2891..3697)
                     /locus_tag="VEO00_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57375.1"
                     /translation="MAQGTQQLPEILTTFEGVAGTLRAEQARFESADTIPTSSIPATT
                     IPWSLVGVGILAIAIGAWMAFTKTRVAAIVAVILGLLLVVVPLALTLPSKASNADVMN
                     DHLRPVYTAQMIDGGTDALSVIGAMGTEMQESMLPALGEQLGMTPEQVQAFLQENLPA
                     TAAGMEAMPGGPAALHEDDRGVRGEPRELHHPEGRLVRADRVDADRVRDHDRGPRGAR
                     PGAHRAQGARPGLRAHADPREGVRLNGTRRTCTGWGAPWTRRPSPRPGGP"
     gene            complement(3661..3984)
                     /locus_tag="VEO00_04920"
     CDS             complement(3661..3984)
                     /locus_tag="VEO00_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57376.1"
                     /translation="MVRRRVAGGFVAAAGAVLIAVAFIAGLFSVGPAFEDLSTGFRPV
                     MTTESINAVTTDLQGLSGVKEELGTVAVPLFSQALRMTPEAFQAFVRSSSRRWPRGPS
                     SCRRS"
     gene            4166..4894
                     /locus_tag="VEO00_04925"
     CDS             4166..4894
                     /locus_tag="VEO00_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57377.1"
                     /translation="MVARGVVTAVLSVLLVGCGGGGATEPFGSAATVSVTTSLTAAAH
                     ASDAALSIYAAWGRGDLETARFYATTEVADFLLNHPFPGDAFAFTGCLPSTEGQGREV
                     CTFAGGTDALDMTFQPDPKGGFRAVGARFYEKTSEGTAASPPSTSPVDTYVAQSPMDA
                     AMGLFSSWASDSPDTALLFATQPAVDTLFGHPYSEPAPTFTGCEPGGGEVTCAYVDEG
                     GAIDMVAEPVGAHYRVREVTFVAD"
     gene            4972..7638
                     /gene="secA"
                     /locus_tag="VEO00_04930"
     CDS             4972..7638
                     /gene="secA"
                     /locus_tag="VEO00_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HYU57378.1"
                     /translation="MAVLGRLQKIGEGRKIKRLQDHVDLVNRLEPDVEKLSDAELKQA
                     SGELRARVSEGDEALDDVLHEAFALVREAARRTLGQRHYDVQVMGAVVLHQGNIAEMK
                     TGEGKTLVSTMPAVLNALAGRGVHVVTVNDYLAKRDSEWMGPIYRALGVSVGLIQSPM
                     RPEQRRPAYGADITYGTNNEFGFDYLRDNMAMRLDDMVQRGHHYAIVDEVDSILIDEA
                     RTPLIISGMATDSAKWYQTFARIVPRLAAGEDYEFDESKRTIAPLEPGVAKVEEILGV
                     ENLYEHVHTPLVHHLQNALRAKELYKRDVDYIVRSGEVLIVDEFTGRVLEGRRYSEGL
                     HQAIEAKEGVRVKAENQTLATITIQNYFRMYEKLAGMTGTASTQAREFEEVYELGVIS
                     IPTNREMVRADRPDEIYKTEDAKWRALVEDIVERHEQGQPFLVGTVSIEKSEKLSGYL
                     QRRGVEHHVLNAKNHEKEAMIVAQAGRIGSVTVATNMAGRGVDILLGGNPEYLARQEM
                     AARGFDNDRYLMFEMDEEERSEYEAEYQPLYRKFKKETDEEHDQVVALGGLYVLGTER
                     HESRRIDNQLRGRSGRQGDPGESKFYLSLQDDLMRLFASERIASIMERLKWPEDEPIT
                     AKIVSRAIESAQGQVEGVNFERRKNILKYDDVKNTQRSIVYEDRLRILEGEDLKDQAL
                     EMVGEVIAGIVAQFANAEVFPEEWDLEGLHGALAAIYPTQLGVERLREFHDANELSET
                     IVQEALEQYAAREAQLGEEVMRQLERIVLLNITDNKWREHLYEIDYLEEGIGLRAYGQ
                     KDPLTEFQREAYHMFDEMKAGIVEEFARYIYRVELIRQDEMERRPRVVTNQPDDGGPQ
                     QPARATSDKIPRNAPCPCGSGRKYKKCHGAVA"
     gene            complement(7661..9061)
                     /locus_tag="VEO00_04935"
     CDS             complement(7661..9061)
                     /locus_tag="VEO00_04935"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYU57379.1"
                     /translation="MMPLSGHRPALRTALALAVVAALAGPAHGAVSAATQTRTRTVPR
                     TLAATASLGALSGPSRVVRPDFPVGYVGVSWASGRVEAVRFEAAGGWTPWRALEPDGD
                     GDRGARTYADLVGGDGAGAFQARGTAGDVRVVALNTTDGPGRPVAAAPEVTASTAGPA
                     IVTRAGWGADESYRFDAGGHEIWHPTFFHLQKMIVHHTDGSNSSTNWAADVRAIYYYH
                     AVTRGYGDIAYNYLIDPLGRLYKGRWSGPAGSRNQSLDTPTAEDARAWSVKAAHAYGA
                     NSGTIGVALLGTFSAVSPTAAARAELAALFAWKSWRHGIDPTAWSTYTNPESGDQWFT
                     ANIGGHRDYVATDCPGDVLYGQLPAIRGAVAAALEDWSGPPGAPMLSARRATGGVRLA
                     WTAPVADGGNPITEYRVYRGTSTGQESLLATVSGATLTHFDTATERFHRYVYEVAAVT
                     VAGEGPPSEEVSARAG"
     gene            9147..9569
                     /locus_tag="VEO00_04940"
     CDS             9147..9569
                     /locus_tag="VEO00_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYU57380.1"
                     /translation="MGELRNVVEALFEVEARGEADVAARLYAPDVEFKGPMIEVHGRD
                     GMKELVESSHRAFPDMKREIITAAEEGDTIAVQIRSKGTHLGPLGFTGGEIPATGRPV
                     DTESAIFIRVENGLVKSWHTFYDGRILMQQLGLAGVGG"
     gene            9649..10765
                     /gene="prfB"
                     /locus_tag="VEO00_04945"
     CDS             join(9649..9714,9716..10765)
                     /gene="prfB"
                     /locus_tag="VEO00_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153759895.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HYU57381.1"
                     /translation="MTEHAERLEDLSRRLTSAKEYLDVAGKEREAAELEGATLAPGFW
                     DDQANAQRVTTRLARLRDDLDRYARLAASLEDLRAIEDLLAEGDDPDLARELADGLTA
                     LERTVERLELAALLSGPYDDNDAVASIHAGAGGTESQDWVEMLLRMYLRWAEREGLEV
                     ELDEVLPGDEAGIKSATFVLKGRYAYGLLSAERGVHRLVRISPFDANKRRHTSFASLD
                     VIPLIEEDDAEIEIDPKDLRIDTYRSSGAGGQHVNVTDSAVRITHLPTGISAACQNER
                     SQMQNRAVAMRILKARLADRARRERLERIEDLRGERRDIEFGSQIRSYVLAPYRLVKD
                     HRTNLEIGDVDRVLDGDLGRLIDAGLRWRAGQGAAAS"
     gene            11152..>12413
                     /locus_tag="VEO00_04950"
     CDS             11152..>12413
                     /locus_tag="VEO00_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HYU57382.1"
                     /translation="MAAIVVLQFLGGPDQARAKQFATLDVLEGTVDVSQGGGAFSEGR
                     DGQSLGEGDVVRTGPQGRAEIEYFDGSMTRLDWSTTFTLEELATLDNAARSKRIVGAQ
                     GAGGKTWSRVSRLTDSQSRFEVRTPTATGAVRGTIFATQCEADGTCLYVVIDGSVVVS
                     SAKGSASLHAFDVVEVQPDGTLGEVEHLSLEELLDLFPWIALNGCVLDNVLVCSVGEN
                     PPPGEEGGGEPPAGPDTGVPTEGDTGTTGDTGIGPESEPPANNVPNAAFDASETSGPA
                     PLEVAFTSRASDRDGDALAHHWSFGDGETSDAPTPTHTFGPGVHAVTLTVTDPDGASD
                     SASLLISASPPGAPVIGEHPPAETTERSATFTFGGSGAAAAGLGLSVAALAPALGFEC
                     SLNGAPFVPCQSPVTYDDLLPGGHAFSVR"
BASE COUNT         1797 a         4479 c         4499 g         1638 t
ORIGIN      
        1 cgccgccggg cggccgacct cttacggagc ccgccgctgc gcgccgcggt cgtcgccatc
       61 ggggttgccc aggcctcgat ggtggcggtg atgggcgtca cgccggtggt catcgacgag
      121 cacggcggcg gggacctggc cgtcgccacc acgatcagct tccacgtggc cgggatgttc
      181 gcgttctccc cgctgatcgg cgcggcactg gaccgctggg gacgcaagcc gggcctcgtg
      241 gcgggcgccc tggtgtcggc cggcggggcg atcctggcgt cggtgaccga caacacgact
      301 gtgatcgcga ccggcgtgtt cctggtcggg ctggggtggt cgggcgcgtt cctggcttcg
      361 accaccatga tcagcgacct gaccaccgcc gaggagcggg ccggcgccct ggggttcacc
      421 gacctgctgg cgtcgctggc ggccgtcgcc ggcgcgctcg cggcggggtt cctgctcgag
      481 ggcgtcggct tctgggtggt cggcgtctcg atggcggcgc tgctcgtgcc ggtgctcgcc
      541 ctggtgctgc cgctgcgcga atcctctccc ggccggtggg gcaccggcgc cgcggcagcc
      601 gcctgagcgg gtggcccgcc gcgtcctgat cgtcgccctc ctgctcgcga tcgcggccgt
      661 ggccctggtc cgcctgcacc gcgaacctcc tcccaggccg cgggagccgt tcgccttcac
      721 gtgcccggcg ccggcgccgt cgccgccggc gctcgccgag ccggcgcccg ccgtcggcgt
      781 caacacgcac ctgctgcgcg acgacacgcg caacgcgacc cgctgcgcgc tgctcgaccg
      841 gctgacggcg gccggcgtga cctggctccg gctcgacctg cggtggtcgg aggcccagcc
      901 gcgcgacccg gcgtccggcg accgcttcgc cgacagcctg gaccgcggcc ggctggcccg
      961 gctcggctcg accgtggacg ccgcccgggc ccgcggccta cgggtgctgg tggtggtgtt
     1021 cgccacgccg cagtgggcga gcgggggacc ggtcgagtcg gccgggccgc cgcttgatgc
     1081 cgccggctac gggcggttcc tcgggttcct cgcgtcccgg taccgcgggc gggtggacgc
     1141 ctggcaggtc tggaacgagc cgaacgagtc cacgttcttc gacgccgacg gcgaggcgtc
     1201 cttctccgcg gtggacgcgg cagcgtacgc cacgctgctc cgccgggccg ccccggccgt
     1261 gcgccggggg gatcccgacg cgctggtcgt cctcggcggg ccctcctaca acaacgttcc
     1321 gtggctggag ctcatctacg cgcagggggt ccgggggctc ttcgacgtca tggccgtgca
     1381 gccgtactcg atcccgtcgt gccgggcgcc cgaggtcgac gacggctcga tctacgtcct
     1441 gcccgcggtg tcgcgggtgc gcgcgttgat ggagcgtgtg ggggacggcg ccctcccgat
     1501 ctggttcacc gagttcggct ggtcctcgca cgcgaacccg ccgctggcgc ccgactaccg
     1561 gctcggcgtc cccgagcagg cgcaggggga ctacctcgtg cgcacgctgg tcctcctccg
     1621 cgagcggtat ccgtacgtgg aggcggcgtt ctggtaccgg gaccgcgatg cggtgatcga
     1681 cgggcaggac ccgacggagg cggcccacct cggcggcttc ggactgctcc ggcgagacct
     1741 ggcggcgaag cccgcgtacc ggacccttcg cgcctacctg ctcacgggga cggtcccggg
     1801 gcgccgcgac gcgccctgcc cggacgccgc cggcgcctga tcaggccgtg ggcgtgacca
     1861 ccaggcggac ggggtcgccg gccttcgacg ccaggcgctc gacgccctcg ttgacgcgcg
     1921 cgagcggcag ccgctcgctg acagacccgg agaggtcgag ccggccggtg gcgatcaggt
     1981 cgaggacggc ctcgaggtgc cgcttgcggt agccgtagtt cccgatcagt gccagcgcgc
     2041 ggaacgagta gaggacgacc ggcccggcct cgatcggttc gagcgactgg ccgacgacga
     2101 ccgcgcggcc gcctcgtgcc agcgatcgca gggcctgctt gacgacggcc gcccgcccga
     2161 tcaggtcgaa cgcggcgtcc agcccgcgcc cgccggcagc agcccggatc gccgcgggga
     2221 cgtcctcgga tgggtcgagg gcgacgtccg cgccgagggc cagggcccgt tcgcgcgccg
     2281 cggggagcgg gtccacggcg gcgacgaagc cggcgcccgc cagacgggcc agctggacgg
     2341 cgtgcgtccc cagcccgccg atgccccaca gcccgacccg gtcgccgggg cggacggcgg
     2401 cccggtcgac gatcgccgag tagacggtgg cgattgcgtc ggcggcgatc gccccgtgct
     2461 cgaacggcac cgcgtccgga agggcggtca gcgagtaggc cgggacgagc acccgctcgg
     2521 cccaggcgcc gtcgtagtgc acgccgagca cccgagggtc ctcgcagtcc tcccagcggc
     2581 cctcgcggca ggcgtggcag gcgccgcacc ccttcccggc cgtgagcgcc acgcggtccg
     2641 cgggcgccca cccctccacg cccggcccga gcgcctcgac gaccccggcc gcctcgtggc
     2701 ccatcgtcac cggggcgggg gcggggagcg gcatctcccc gaggatgaag tgcaggtccg
     2761 aagcacagat cccgcaggcc tcgacccgga cgaccgcctc tccggccccc ggcacggggt
     2821 cgggtacctc ctccaggcgc aatggctcgt tcgccgcgtg gaaccgggcg gcccgcatgt
     2881 cgggcgcagt ctacgggccg cccgggcgcg gcgaggggcg ccgcgtccac ggcgcccccc
     2941 acccggtgca ggtgcggcgg gttccgttca gacggacacc ttctcgcgga tcggcgtggg
     3001 ctcgaaggcc ggggcgggct ccttgcgcgc gatgcgcacc aggccgagcg ccccgaggac
     3061 cgcgatcgtg atcccggacc cgatcagcgt ccacacgatc ggcacgaacg agacgtcctt
     3121 cagggtggtg tagttcgcga ggttcgcctc gaacaccccg atcatcttcg tgaagcgctg
     3181 cagggccccc gggcatcgcc tccatgcccg cggcggtcgc cggcaggttc tcctgcagga
     3241 aggcctgcac ctgctcgggg gtcatgccga gctgctcgcc gagcgcgggg agcatcgact
     3301 cctgcatctc ggtgcccatc gccccgatca cggagagcgc gtccgtgccg ccgtcgatca
     3361 tctgcgcggt gtagaccggc cgcaggtggt cgttcatcac gtcggcgttg gacgccttgg
     3421 acggaagggt cagcgccagc ggcacgacca ccaggagcag ccccaggatg acggccacga
     3481 tcgccgccac acgggtcttg gtgaacgcca tccaggcgcc gatcgcgatg gccaggatcc
     3541 cgaccccgac cagggaccac ggaatcgtgg tggccgggat cgacgacgtc gggatggtgt
     3601 cggccgactc gaagcgggcc tgctcggccc gcagcgtccc ggcgacgccc tcgaacgtgg
     3661 tcaggatctc cggcagctgc tgggtcccct gggccaccgc cgggaactgc tccgcacgaa
     3721 ggcctggaac gcctcggggg tcatccgcag cgcctgcgag aacagcggca ccgccaccgt
     3781 gcctaactcc tccttcaccc ccgagagccc ttgcaggtcg gtggtcacgg cgttgatcga
     3841 ctcggtggtc atcaccggcc ggaagccggt cgacaggtcc tcgaacgccg gaccaacgct
     3901 gaacaggccg gcgatgaacg caaccgcgat caggaccgct cccgcggcgg ccacgaagcc
     3961 gccggcaacc cgccgacgca ccatctggaa ctccctcctc ccgcgagggc cgtgcccacg
     4021 gccgtcgaca tggccgccgc ccacacggcc gcaaccagtc tgcgttgggc ccggtgccgg
     4081 gtcgcggggc gatggtcccg gcccgcgagg gcttcgctcg cgccctggac gggggcgcgg
     4141 gtgggtgcga tgctcgggca gtcggatggt cgcgcgtggc gtggtcacgg cagtgctgtc
     4201 cgtcctcctc gtagggtgtg ggggaggcgg ggcgaccgag ccgttcggct ccgcggcgac
     4261 cgtgtccgtc acgacgtcgc tgaccgcggc ggcgcacgcc agcgacgccg ccctgtcgat
     4321 ctacgccgcc tgggggcggg gcgacctcga gacggcgcgg ttctacgcca ccacggaggt
     4381 ggccgacttc ctgctgaacc atcccttccc gggcgacgcg ttcgcgttca ccggctgtct
     4441 cccctccacg gagggccagg gccgcgaggt ctgcacgttc gccggtggca ccgacgcgct
     4501 ggacatgacc ttccagccgg accccaaggg cggcttccgc gccgtcggcg cgcgcttcta
     4561 cgagaagacc agcgagggaa cggccgccag cccgccgtcc acctcgccgg tggacacgta
     4621 cgtggcgcag agcccgatgg acgccgccat gggtctgttc agctcgtggg cctccgacag
     4681 cccggacacg gcgctgctgt tcgccacgca gccggcggtc gacaccctgt tcgggcaccc
     4741 gtactcggag cccgcgccga cgttcaccgg atgcgagccg ggcggcggcg aggtgacctg
     4801 cgcctacgtc gacgagggcg gggcgatcga catggtcgcc gagcccgtcg gcgcgcacta
     4861 ccgcgttcgc gaggtcacct tcgtggcgga ctgaccccgc cacggggggc tctgcccccc
     4921 gtgaaccccc aagacctcgt tccggggacc accgcctaga atcgagcgaa cgtggcggta
     4981 ctcggcaggc tgcagaagat cggcgagggg cgcaagatca agcgcctgca ggaccacgtc
     5041 gacctcgtca accggctcga gcccgacgtg gagaagctct ccgacgccga gttgaagcag
     5101 gcgtcggggg agctccgggc gcgggtgagc gagggcgacg aggcgctcga cgacgtcctg
     5161 cacgaggcct tcgcgctggt gcgcgaggcc gcccggcgca ctctcggcca gcgccactac
     5221 gacgtccagg tgatgggcgc cgtcgtcctg caccagggca acatcgccga gatgaagacc
     5281 ggtgagggca agacgctcgt ctcgaccatg ccggccgtcc tcaacgcgct ggccggccgg
     5341 ggcgtgcacg tcgtcaccgt caacgactac ctggccaagc gcgactcgga atggatgggc
     5401 cccatctacc gcgcgctcgg cgtgtcggtc gggctgatcc agtccccgat gcgtcccgag
     5461 cagcgccgcc cggcctacgg cgccgacatc acgtacggga cgaacaacga gttcgggttc
     5521 gactacctgc gcgacaacat ggcgatgcgc ctggacgaca tggtccagcg cggccaccac
     5581 tacgcgatcg tcgacgaggt cgactcgatc ctcatcgacg aggctcgcac cccgctgatc
     5641 atctccggga tggcgaccga ctcggccaag tggtaccaga ccttcgcccg catcgttcct
     5701 cgcctcgcgg ccggcgagga ctacgagttc gacgagagca agcggacgat cgcgccgctc
     5761 gagccgggcg tggcgaaggt cgaggagatc ctcggcgtcg agaacctcta cgagcacgtc
     5821 cacacgccgc tcgtccacca cctgcagaac gccctgcggg ccaaggagct ctacaagcgt
     5881 gacgtggact acatcgtccg cagcggcgag gtgctgatcg tcgacgagtt caccggccgc
     5941 gtgctggagg gccggcgcta ctccgagggc ctgcaccagg cgatcgaggc gaaggagggc
     6001 gtgcgggtca aggccgagaa ccagacgctc gccacgatca ccatccagaa ctacttccgg
     6061 atgtacgaga agctcgccgg catgaccggc accgcctcga cccaggcccg cgagttcgaa
     6121 gaggtctacg agctcggcgt gatctcgatc ccgaccaacc gcgagatggt ccgagcggac
     6181 cggcccgacg agatctacaa gaccgaggac gccaagtggc gggccctggt cgaggacatc
     6241 gtcgagcgcc acgagcaggg ccagccgttc ctggtcggca ccgtctcgat cgagaaatcc
     6301 gagaagctct ccggctacct gcagcgccgc ggcgtcgagc accacgtcct gaacgcgaag
     6361 aaccacgaga aggaggcgat gatcgtcgcc caggccgggc ggatcggctc ggtcacggtg
     6421 gccacgaaca tggccgggcg gggcgtggac atcctcctcg gcggcaatcc cgagtacctg
     6481 gcgcgccagg agatggcggc gcgcggcttc gacaacgacc gctacctgat gttcgagatg
     6541 gacgaggagg agcggtcgga gtacgaggcc gagtaccagc cgctctaccg taagttcaag
     6601 aaggagaccg acgaggagca cgaccaggtc gtggcgctcg gcggcctgta cgtcctgggg
     6661 accgagcggc acgagtcgcg gcgcatcgac aaccagctgc gcggccgctc cggccggcag
     6721 ggcgacccgg gcgagtcgaa gttctacctc tcgctgcagg acgacctgat gcgcctgttc
     6781 gcgtcggaac gcatcgcctc gatcatggag cgcctgaagt ggccggagga cgagccgatc
     6841 accgcgaaga tcgtgtcgcg ggcgatcgag tcggcacagg gccaggtgga gggcgtcaac
     6901 ttcgaacgcc gcaagaacat cctgaagtac gacgacgtca agaacaccca gcgctcgatc
     6961 gtctacgagg accgcctgcg gatcctggag ggcgaggacc tcaaggacca ggcgctggag
     7021 atggtcggcg aggtcatcgc cgggatcgtg gcgcagttcg cgaacgccga ggtcttcccg
     7081 gaggagtggg acctcgaggg cctgcacggc gcgctggccg cgatctaccc gacgcagctc
     7141 ggcgtggaac ggctccggga gttccacgac gcgaacgaac tctccgagac gatcgtccag
     7201 gaggcgctcg agcagtacgc cgcccgcgag gcgcaactcg gcgaggaagt catgcgccag
     7261 ctggagcgga tcgtcctgct gaacatcacc gacaacaagt ggcgcgagca cctctacgag
     7321 atcgactacc tcgaggaggg gatcgggctc cgcgcctacg ggcagaagga cccgctgacg
     7381 gagttccagc gcgaggcgta ccacatgttc gacgagatga aggccgggat cgtcgaggag
     7441 ttcgcgcgct acatctaccg cgtggagctg atccgccagg acgagatgga gcgccgcccg
     7501 cgcgtcgtga cgaaccagcc cgatgacggc ggcccgcagc agcccgcccg cgccacgagc
     7561 gacaagatcc cgcgcaacgc cccctgcccc tgcggctccg ggcgcaagta caagaagtgc
     7621 cacggcgccg tcgcctgacg gccaccccgc accgtctgcg ctagccggct cgcgccgaga
     7681 cctcctccga gggcggcccc tcgccggcga cggtcacggc cgcgacctcg tagacgtagc
     7741 ggtggaaccg ctccgtggcg gtgtcgaagt gcgtgagcgt cgcgccgctc accgtggcga
     7801 ggagcgactc ctggcccgtg gacgtccccc ggtagacccg gtactcggtg atcgggttgc
     7861 ccccgtcggc gaccggcgcg gtccacgcga ggcggacgcc gccggtggcc cggcgcgcgg
     7921 agagcatcgg cgcgccggga ggtccggacc agtcctcgag ggccgcggcc accgcgcccc
     7981 ggatcgccgg cagctggccg tagagcacgt cacccggaca gtccgtcgcc acgtagtccc
     8041 gatgcccgcc gatgttcgcg gtgaaccact ggtcgccgct ctccgggttc gtgtaggtcg
     8101 accacgcggt cgggtcgatc ccgtggcgcc aggacttcca cgcgaacagg gccgcgagtt
     8161 cggcccgggc ggccgcggtc ggcgagacgg cggagaaggt gccgagcagc gcgacgccga
     8221 tggtgccgga gttcgccccg taggcgtggg cggccttcac cgaccaggcc cgcgcatcct
     8281 cggccgtcgg cgtgtccagg ctctggttcc ggctccctgc cggccccgac cagcgcccct
     8341 tgtagagccg cccgagcggg tcgatcaggt agttgtaggc gatgtcgccg tagccccgcg
     8401 tgacggcgtg gtagtagtag attgcccgca cgtccgccgc ccagttcgtc gagctgttcg
     8461 agccgtccgt atggtggacg atcatcttct gcaggtggaa gaacgtcggg tgccagatct
     8521 cgtggccgcc ggcgtcgaac cggtaggact cgtcggctcc ccatcccgcc cgggtgacga
     8581 tcgccggccc cgccgtggag gcggtgacct ccggcgcggc ggcgaccggt cgccccggtc
     8641 cgtccgtcgt gttgagcgcg acgacgcgaa cgtcgccggc cgtcccccgg gcctgaaacg
     8701 cgccggcccc gtcgccaccg accaggtcgg cgtaggtgcg ggctccgcga tcgccgtcgc
     8761 cgtccggctc cagcgctcgc cagggcgtcc atccgccggc ggcctcgaag cgcacggcct
     8821 ccacccggcc cgacgcccac gacacgccga cgtacccgac cgggaagtcg gggcgcacca
     8881 cgcggctggg ccctgacagc gctccgagcg acgcggtcgc cgcgagggtt cgcggaacgg
     8941 tccgcgtccg cgtctgcgtc gccgcggaca cggcgccgtg cgccggcccg gcgagcgccg
     9001 cgacgacggc gagggccagc gccgtgcgca gcgcagggcg gtgtcccgag agcggcatca
     9061 tgcgccgatc atccgacgac ggcggccggc tgtccatcac cggatagact gcggcacctc
     9121 acggctcggc gaacggaggc gatcggatgg gagagctgcg gaacgtggtc gaggcgctgt
     9181 tcgaggtcga ggcgcgcggc gaggctgacg tcgccgcgcg gctgtacgcg ccggacgtcg
     9241 agttcaaggg tccgatgatc gaggtccacg gccgcgatgg catgaaggag ctggtggagt
     9301 cctcgcatcg ggccttcccg gacatgaagc gcgagatcat caccgccgcc gaggaggggg
     9361 acacgatcgc ggtccagatc cgctcgaagg gcacgcacct cggcccgctc gggttcacgg
     9421 ggggcgagat cccggcgacc gggcgacccg tcgacaccga gtcggccatc ttcatccgcg
     9481 tcgagaacgg cctggtgaag tcctggcaca cgttctacga cggccggatc ctgatgcagc
     9541 agctgggatt ggcgggtgtg ggggggtagc gcgccctggg gcgtgatctg ctcaaccacc
     9601 gatggcgagc ggatccccac gagaggaccc ggccagtaac atcgacgcgt gaccgagcac
     9661 gccgaacgcc tggaggatct ctctcgccgc ctgaccagcg cgaaggagta cctttgacgt
     9721 cgccgggaag gagcgcgagg cggcggagct cgagggcgcc acgctcgcgc ccggcttctg
     9781 ggacgaccag gcgaacgcac agcgggtgac cacccgcctg gcgcggctgc gggacgacct
     9841 cgaccgctac gcccggctgg ccgcgtccct cgaggacctg cgcgccatcg aggatctgct
     9901 ggccgagggg gacgaccccg acctggctcg cgagctggcc gacgggctga ccgccctgga
     9961 acgcacggtc gagcgcctcg agctcgccgc gctcctctcc ggcccttacg acgacaacga
    10021 cgccgtggcc tcgatccacg cgggggccgg cggcacggag agccaggact gggtcgagat
    10081 gctcctgcgc atgtacctgc gctgggccga gcgcgagggg ctcgaggtgg agctcgacga
    10141 ggtcctgccc ggggacgagg ccgggatcaa gagcgcgacg ttcgtgctga aggggcgcta
    10201 cgcgtatggc ctgctgtcgg ccgagcgcgg ggtgcaccgg ctggtgcgca tctcgccctt
    10261 cgacgcgaac aagcgccgcc atacctcgtt cgccagtctc gacgtcatcc ccctgatcga
    10321 ggaggacgac gcggagatcg agatcgaccc gaaggacctg cggatcgaca cctaccggtc
    10381 cagcggcgcc gggggccagc acgtcaacgt caccgactcc gccgtgcgga tcacccacct
    10441 cccgaccggg atctccgccg cctgccagaa cgagcgctcg cagatgcaga accgcgccgt
    10501 ggcgatgcgc atcctgaagg cccgcctggc cgaccgggcc cgccgggagc gcctggagcg
    10561 gatcgaggac ctccgcggcg agcgccggga catcgagttc ggctcccaga tccggtccta
    10621 cgtgctcgct ccgtatcgcc tcgtgaagga tcaccggacg aacctcgaga tcggcgacgt
    10681 ggaccgggtc ctcgacggcg acctgggacg gctgatcgac gccggccttc gctggcgtgc
    10741 ggggcagggg gccgcggcct cgtagcggac cgatccggcc ccccggccgc ggcgaacgtc
    10801 cccatatggc gcctcggata tgggtgacga acggcaccgg ccggcggtga aggcgctcac
    10861 atcggcgccc ggcctcctga gcacagaatc ggtcgcggca ggccggcgag gggtcggccc
    10921 gaagggggac ccggagatgt tccgcccaaa cggaccgaac gcggcataaa tctccgggct
    10981 ggtggcagaa atcccttgcc agaaggcccg acaagacgag acaatcacgc ttggcgcgtc
    11041 cgggcgtctc ggtttccccg aaccccctca cgccaacccg cgcgaagggg tcctctcgtg
    11101 agcacggccc agaaacggtg gttctctctc ggcacgatac tggccgtctt ggtggcggcc
    11161 atcgtcgtgc tgcagttcct gggcgggccc gaccaggcgc gcgccaagca gttcgccacg
    11221 ctcgacgtcc tggaggggac ggtcgacgtc agccagggcg gcggggcgtt ctccgagggg
    11281 cgcgacgggc agtcgctcgg cgagggggac gtggtccgca ccggtccgca gggccgggcc
    11341 gagatcgagt acttcgacgg ctccatgacc cgcctcgact ggagcacgac cttcacgctg
    11401 gaggagctgg ccacgctcga caacgccgcg cggtcgaagc ggatcgtcgg cgcgcagggc
    11461 gcgggcggca agacctggag ccgcgtctcg cggctcaccg actcgcagag ccgcttcgag
    11521 gtccgcactc cgacggcgac cggcgccgtt cgcgggacca tcttcgccac gcagtgcgag
    11581 gcggacggga cctgtctgta cgtggtgatc gacgggagcg tcgtggtcag ctccgcgaag
    11641 gggtcggcga gccttcacgc gttcgacgtg gtcgaggtgc agccggacgg gacgctgggc
    11701 gaggtggagc acctgtcgct cgaggagctc ctggacctgt tcccgtggat cgcgctgaac
    11761 ggctgcgtcc tcgacaacgt gctcgtctgc tcggtgggcg agaacccgcc gcccggcgag
    11821 gagggcgggg gcgagccgcc ggccggcccg gacaccggcg tgccgaccga gggcgacacc
    11881 ggcaccacgg gtgacacggg gatcggcccg gagagcgagc cgccggcgaa caacgtgccg
    11941 aacgcggcgt tcgacgcgtc cgagacctcc ggtcccgctc cgctcgaggt ggcgttcacc
    12001 agccgggcat cggaccgcga cggcgacgcc ctcgcgcacc actggagctt cggcgacggg
    12061 gagacctccg acgcgcccac cccgacgcac accttcggtc caggggtcca cgccgtgacg
    12121 ttgaccgtca ccgaccccga cggcgcctcc gactcggcgt cgctcttgat ctccgcgtcg
    12181 ccgcccggtg cgccggtgat cggcgagcac ccgccggcag agaccaccga gcggtcggcg
    12241 acgttcacct tcggcgggtc cggtgcggcc gccgccggcc tcgggctctc ggtcgccgcg
    12301 ctcgcgccgg ccctcgggtt cgagtgcagc ctcaacggcg cgccgttcgt gccctgccag
    12361 tcgccagtca cgtacgacga cctgcttccc ggcgggcatg cgttctccgt ccg
//
LOCUS       DASPWO010000222         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_624699, whole genome shotgun sequence.
ACCESSION   DASPWO010000222 DASPWO010000000
VERSION     DASPWO010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624699"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VEO00_04955"
     CDS             complement(<1..808)
                     /locus_tag="VEO00_04955"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HYU57383.1"
                     /translation="MEPDAAQRTLDQELQRGLELIAQADSVDAVERARQAVLGRKSAF
                     SEVQRSLGSFSEQDRRSIGHRANEVRDALEQGIRQRREALAESEELERLERDRVDVTL
                     PGRRPRPGAMHPLAVTEHEIVDIFTRMGYRVVEGNEVETDWYNFTALNIPPAHPARTE
                     KDTIFLDIPGHDDLLLRTETSAMQIRTMEKQEPPVFVIAPGRVFRQETPDPTHTPVFH
                     QVEGLAVDEGITFADLKGTLHAFAKAMFGEDTRVRMTPDFFPFVEPGAQLA"
     gene            complement(822..>2258)
                     /locus_tag="VEO00_04960"
     CDS             complement(822..>2258)
                     /locus_tag="VEO00_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HYU57384.1"
                     /translation="LKNPRVAEALKLRKRGLREDRRQFLVEGPQAVGEAIGRRPGGAL
                     SVLFHTPAEGLHPVVGRARDAGVPLVEVSEEVVRHLTSTVTPQGLVGVAGFVDVSLAE
                     LPADVSLAAVLYEVRDPGNAGTVLRSADAAAADAVVFTGSSVDVYNPKTVRSSAGSLF
                     HLPVVRDAAVQDAVTELKNRGLAVYAATAAGDRDLYSLDLAAPAAFLFGNEAWGLPDE
                     VAALADASVRVPISPRAESLNLAAAASVFLFEAVRQRTAGGADLASLVAGAAHDIRSP
                     LTAARGFATTLVSRWRTMGDEDREAMLRAIAHDAGRMNAILRELVDAARATTGHLELS
                     PERVELGEVVRAAVEAVAVRPDFPAVDTRIDEGRALVDRARLQGAVEAMLEAAAWWGE
                     EGPIRLEVGSRSRSSGNAVDVEVSRESTSLTRDEAAALLAPRRAGSGGGSKLGLFVAR
                     AVAEAHGGTLTVDVEGGIRLRLVLPGGG"
BASE COUNT          301 a          853 c          804 g          300 t
ORIGIN      
        1 cggccagctg cgcgcccggc tccacgaacg ggaagaagtc gggggtcatc cggacccgcg
       61 tgtcctcgcc gaacatggcc ttcgcgaagg cgtggagcgt tcccttcagg tcggcgaacg
      121 tgatgccctc gtcgacggcc agcccctcca cctggtggaa caccggcgtg tgggtggggt
      181 cgggcgtctc ctggcggaag acgcggccgg gcgcgatcac gaagaccggg ggctcctgct
      241 tctccatggt tcggatctgc atggccgagg tctcggttcg cagcagcagg tcgtcgtgcc
      301 cggggatgtc gaggaagatg gtgtccttct cggtgcgggc cggatgggcc ggcgggatgt
      361 tcagcgccgt gaagttgtac cagtcggtct ccacctcgtt cccctcgacg acgcggtagc
      421 ccatgcgcgt gaagatgtcg acgatctcgt gctcggtgac ggcgaggggg tgcatcgcgc
      481 cggggcgagg acggcgtccc gggagggtga cgtcgacacg gtcgcgctcg agccgttcga
      541 gctcctccga ttccgcgagc gcctcccggc gctgccggat gccctgctcg agcgcatccc
      601 ggacctcgtt ggcccggtgg ccgatcgagc gccggtcctg ctcggagaac gaccccagtg
      661 agcgctggac ctccgagaac gccgacttgc gtccgaggac ggcctgccgg gcccgctcca
      721 cggcgtccac cgagtcggcc tgggcgatga gctccaggcc gcgctggagc tcctgatcga
      781 gggtgcgctg cgccgcgtcc ggttccatgg gtccggccag tttagccccc gcccgggagg
      841 accagcctca gccggatacc gccctcgacg tccacggtca gcgtcccgcc gtgggcttcc
      901 gccacggccc gggccacgaa caggccgagc ttgctccccc cgccggaccc ggccctgcgc
      961 ggcgcgagga gcgcggctgc ctcgtcccgc gtcagcgagg tgctctcccg ggagacctcc
     1021 acgtcgaccg cgttcccact cgagcgcgac cgtgagccca cctcgagccg gatcggcccc
     1081 tcctcgcccc accacgccgc ggcctcgagc atcgcctcga ccgctccctg gaggcgagcg
     1141 cggtccacga gcgcccggcc ctcatcgatc cgcgtgtcca cggcggggaa gtccgggcgc
     1201 actgcgacgg cctcgaccgc cgcccggacg acctccccca gttccacgcg ctcgggcgac
     1261 agctcgagat gcccggtcgt ggcccgggcg gcgtccacca gctcgcgcag gatcgcgttc
     1321 atccgcccgg cgtcgtgcgc gatggcccgg agcatcgcct cccggtcctc gtcgcccatc
     1381 gtccgccacc tggagacgag ggtcgtcgcg aagccgcggg cggccgtgag gggagaccgg
     1441 atgtcgtggg cggctccggc cacgagcgag gccaggtccg cgcccccggc ggtgcgctgc
     1501 cggacggcct cgaacaggaa cacggacgcc gccgcagcga ggttcagcga ctccgcgcgc
     1561 ggcgagatcg ggacccgaac cgaagcgtcg gcgagggcag cgacctcgtc gggcagcccc
     1621 caggcctcgt tgccgaacag gaacgcggcc ggcgccgcca ggtcgagcga gtacaggtcg
     1681 cgatccccgg cggcggtcgc cgcgtacacg gccaggcccc ggttcttcag ctcggtcacg
     1741 gcgtcttgga cggccgcgtc gcgcaccacc ggcaggtgga acagcgaccc ggccgaggac
     1801 cgaacggtct tggggttgta cacgtccacg gaagaccccg tgaagacgac cgcgtccgcc
     1861 gccgccgcgt cggcggagcg cagcaccgtc ccggcgttcc cgggatcccg cacctcgtag
     1921 agcacggccg cgagggacac gtctgctggg agctccgcga gcgagacgtc gacgaacccg
     1981 gccacgccga ccaggccctg cggcgtcacg gtcgacgtga ggtggcggac gacctcctcg
     2041 ctcacctcca ccagtggaac gcctgcgtcc cgggcccgcc cgaccacggg gtgcaggccc
     2101 tcggccggcg tgtggaacag caccgagagc gctcccccgg gccggcggcc gatggcctcc
     2161 cccacggcct gggggccctc caccaggaac tgccggcggt cctcgcgaag ccctcgcttg
     2221 cgaagcttca gcgcctcggc cacgcggggg ttcttcag
//
LOCUS       DASPWO010000223         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_454784, whole genome shotgun sequence.
ACCESSION   DASPWO010000223 DASPWO010000000
VERSION     DASPWO010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454784"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1142
                     /locus_tag="VEO00_04965"
     CDS             141..1142
                     /locus_tag="VEO00_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU57385.1"
                     /translation="MRFGLALPHYGFSFPEGGPLDWGRLVDHARRAEALGFDSVWISD
                     HFFLDLARYGGPPEPHAAAEPFTALAGIAVATRRVRLGTLVACTGFRHPAHVVKMATT
                     LDLLSEGRMELGLGAGWYEREFRAFGYPFGTAGERFDRLEEFLAVARALFGEGEPVDH
                     EGRHFRLHRAYNHPRPAAAGRPPIWVGSKGKPRSARLAARYADGWNTVWRWAPQDYRA
                     AARAMDAACEAEGRDPATLRRSLGLHTLVGENDRDLRARYEALRSWTPGRSLDPEPLE
                     SFAAGNLVGTVDAVREQVAAWEARGVEELIVSLASLPFAVADGSLADLFAEGVIHAA"
     gene            1190..>2018
                     /locus_tag="VEO00_04970"
     CDS             1190..>2018
                     /locus_tag="VEO00_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU57386.1"
                     /translation="MSGPADPPDLREERKVVTVVFADLVGSTALAERLDPEEVRLVVG
                     EAMARMVSIVDDLGGTVKDLAGDGVLALFGAPVAHEDDAERAVRAGMRIAAEVGAYAA
                     EVERSWGVGGFDVRVGVETGPVVLGPVGAGSRVEYAAFGDTVNTAARIQSEAEPGGVL
                     VGAGTRRLVEPLFTWSEPRALTVKGKARPVRASAVTGVGAPHPRLRGLGGVTVSIVGR
                     GRELETAGQLVEAVLAGAGGALFVTGEAGIGKTRLVAEIRERVERSGAARGRPTWLEG
                     "
BASE COUNT          220 a          714 c          813 g          271 t
ORIGIN      
        1 ggtggtctac gagggagcgc acctcgccga ccacgtctcc gtctacccgc gcctgagggt
       61 cccggccgtg ctgcgcgtgc cgcccgggat cgagctgacc gacgccgacg acgtcctgaa
      121 gtacctgtag cctcgcccgg atgcggttcg ggctggcgct gccccactac gggttctcgt
      181 tccccgaggg cggtcccctg gactgggggc ggctggtcga ccacgcccgg cgggccgagg
      241 cgctcggctt cgactccgtg tggatctccg accacttctt cctcgacctc gcccggtacg
      301 gcggtccgcc cgagccgcac gcggcggcgg aacccttcac ggcgctggcc gggatcgccg
      361 tcgccacgcg ccgcgtccgg ctggggacgc tcgtcgcctg caccgggttc cgccacccgg
      421 ctcacgtggt gaagatggcg acgacgctcg acctcctctc cgaggggcgg atggagctcg
      481 gactgggcgc cggctggtac gagcgggagt tccgggcgtt cggctacccg ttcgggacgg
      541 ccggggagcg gttcgaccgg ctcgaggagt tcctcgccgt cgcccgggcg ctgttcggag
      601 agggcgaacc ggtcgaccac gagggccggc acttccggct gcaccgggcc tacaaccacc
      661 cgcgtccggc ggcggcggga cgcccgccga tctgggtcgg gtcgaagggg aagccccgca
      721 gcgcgcgcct ggccgcccgc tacgcggacg gctggaacac cgtgtggcgc tgggcccccc
      781 aggactaccg ggccgcggcg cgggcgatgg acgccgcctg cgaggccgag gggcgtgacc
      841 cggccacgct ccggcgctcg ctgggcctcc acacgctcgt cggcgagaac gaccgggacc
      901 tgcgggcccg gtacgaggcg ctgcggtcct ggacgcccgg ccgctccctc gatccggagc
      961 cgctcgagtc cttcgctgcc ggcaacctgg tcggcaccgt cgacgcggtg cgcgagcagg
     1021 tggccgcctg ggaggcgcgg ggcgtcgagg agctgatcgt gtcgctggcc tcgctgccgt
     1081 tcgccgtcgc cgacggctcg ctcgccgacc tgttcgccga gggcgtgatc cacgcggcct
     1141 gaggtccggg tccttcccgc gtcgtcggga gggaccacaa tgtcctccca tgtccggccc
     1201 ggccgatccc ccggacctgc gcgaggagcg caaggtcgtc acggtggtgt tcgcggacct
     1261 ggtcgggtcg acggcgctcg ccgagcggct cgaccccgag gaggtccgcc tggtcgtcgg
     1321 cgaggcgatg gcccggatgg tgtcgatcgt cgatgatctc ggcggcaccg tgaaggacct
     1381 cgccggggac ggcgtgctcg cgctgttcgg cgcgccggtg gcgcacgagg acgacgccga
     1441 gcgcgccgtg cgcgcgggga tgcgcatcgc ggccgaggtc ggagcgtacg cggccgaggt
     1501 cgagcggagc tggggggtcg gcggattcga cgtccgggtg ggcgtcgaga ccgggccggt
     1561 ggtgctcggg ccggtcggcg cggggagccg ggtggaatac gcggccttcg gcgataccgt
     1621 gaacaccgcg gcgcggatcc agtccgaggc agagccgggg ggcgtcctgg tcggggcggg
     1681 gacccggcga ctggtggagc cgctgttcac ctggagcgag ccccgtgccc tcacggtgaa
     1741 gggcaaggcg cggccggtcc gcgcgtcggc ggtgaccggg gtcggggcgc cccacccacg
     1801 cctgcgcggc ctgggcgggg ttaccgtgtc gatcgtcggg cgcgggcgcg agctcgagac
     1861 ggccgggcag ctcgtcgagg ccgtcctcgc cggggccggc ggcgcgctgt tcgtcaccgg
     1921 cgaggcgggg atcggcaaga cgcggctggt cgccgagatc cgggagcgcg tcgagcggtc
     1981 cggggcggcc cgcgggcgtc cgacctggct cgagggac
//
LOCUS       DASPWO010000224         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_114951, whole genome shotgun sequence.
ACCESSION   DASPWO010000224 DASPWO010000000
VERSION     DASPWO010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114951"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VEO00_04975"
     CDS             complement(<1..1319)
                     /locus_tag="VEO00_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYU57387.1"
                     /translation="MATTDRTATTRPRLGDRMPGRARTGARRAGWWRGWFAPAGGLRP
                     RLVDVLVALVIAVVQVAGTIAYSHDEPPSPWAFDAFAGVLLIAGPVALLVRRRWPVAT
                     LVVAFAAAAGYTAGGYPRGPAGLPAFAFAMVSAIMMGRRALAWGVLVVAYPAFTLLPS
                     LLPDEVEKARGLADAAANLLWLPLVGAATEIARSRLERRAERAHVAREEARRRASEER
                     LLIARELHDVLAHHISLINVQSGVALHLLEERPEQARPALQAINEASDEALAELRSVL
                     EVLSRGLAEDGAHGESGAWAPRAPTAGLGDLDGLVRRTRAAGLDVHLAVEGEARPVPA
                     RVDLAAFRIVQEALTNVVRHAGGDARATVRLVYASAELVVQVDDDGRGADPPAAGSGG
                     STTAGKGAGRGRGIAGMRERVHALDGTFTAGPRPGRGFRVRARFPLR"
     gene            1594..2055
                     /locus_tag="VEO00_04980"
                     /pseudo
     CDS             1594..2055
                     /locus_tag="VEO00_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
BASE COUNT          301 a          861 c          774 g          265 t
ORIGIN      
        1 tcccgtagtg ggaaccgcgc ccggacgcgg aagccccggc cgggacgggg gccggcggtg
       61 aacgtcccgt ccagggcgtg gacccgctcc cgcatgcccg cgatgccgcg gccacggccc
      121 gcgcccttgc ccgccgttgt cgagccgccc gacccggcgg caggcgggtc ggcgccccgg
      181 ccgtcgtcgt cgacctgcac gaccagctcg gcggaggcat acaccaggcg gaccgttgcc
      241 ctggcgtcgc cgccggcgtg gcggacgacg ttggtgagcg cctcctgcac gatgcggaac
      301 gccgccaggt cgaccctggc gggcaccggc cgggcctcgc cctcgacggc caggtggacg
      361 tcgaggccgg ccgcccgggt ccgtcgcacc aggccgtcga ggtcgcccag gccggccgtg
      421 ggagcgcggg gcgcccacgc cccggactcg ccgtgggccc cgtcctcggc gaggccgcgg
      481 ctcagcacct caagcaccga gcgcagctcc gccagcgcct cgtcgctcgc ctcgttgatg
      541 gcctgcaggg cgggccgggc ctgctcgggc cgctcctcca gcaggtgcag cgccacgccc
      601 gactgcacgt tgatgagcga gatgtggtgg gccagcacgt cgtgcagctc ccgggcgatc
      661 agcagccgct cctcgctcgc gcgacgccgg gcctcctccc gcgccacgtg agcccgctcg
      721 gcacgccgct cgaggcggct tcgcgcgatc tcggtggcgg cgccgaccag cggcaaccag
      781 aggaggttcg cggccgcgtc ggcgaggccg cgggccttct ccacctcgtc gggaagcagg
      841 gacggcagca gggtgaaggc cgggtaggcg accaccagca caccccacgc gagcgcgcgg
      901 cggcccatca tgatggcgct caccatggcg aacgcgaagg cggggagccc ggccggccca
      961 cgcgggtagc caccagcggt atagcccgcg gccgccgcga aggcgaccac cagggtggcc
     1021 acgggccagc gtcggcgaac cagcaacgcc accgggccgg cgatgagcag cacgcctgcg
     1081 aacgcgtcga acgcccaggg gctgggcggc tcgtcgtggc tgtaggcgat cgtgccggcg
     1141 acctggacga cggcgatcac cagggcgaca agcacatcca ccagccgggg ccgcagcccc
     1201 ccggccggcg cgaaccagcc ccgccaccac ccggcccgac gggcgccggt ccgggcgcgc
     1261 ccgggcatcc ggtcgccaag gcggggccgg gtggtggcag tgcggtcggt ggtggccacg
     1321 ccagcacggt aagccgccgc tcacccgccc gacaacgtcc ccggggagta acgccacctc
     1381 ctccgccggt agtaccagcg gcagcggacg gagtaaccac tcccgggttt cccgagcccc
     1441 tcatgtcgcc gcggggccgg cgcctccggc aacgcgcccc ccttcccgga cacctgagtt
     1501 ctcagtttca gacgcgctct cagtcgacgt ccgacccggc gcggcctggg tcgagccggc
     1561 cgactacgtc cagttcgtcc gcggcacctt caacatgtcg ctgacgccac cgcgctgcct
     1621 ctcgacgacg gctcggtcac cggctaccgc gccgacaagg tcctgcacgc cctcgccgag
     1681 cccgaacgag ccgtcgtcga ggcccgccgg gtcctggcca ggaacggccg tgcggtgctc
     1741 gtcggccacg actgggacac gttcgtgatc gactccgacg atcccgaact cacccgcacg
     1801 ctcgtccacg ctcgcgcgga cggcatgcca aacccgcggg tcgctcgccg ataccggaac
     1861 ctcctgctgg acaacgggtt cgccaacgtc accgtcgagg tccacacgat cgtgtggacc
     1921 gacgccgcgt ctctgccgat gctcgccact atcggagaag gctcctggct cgacgaccag
     1981 gccgccaggg cacgcaacga ccggctgttc gtcgccgtcc cggtctttct cgcctccggg
     2041 actcgcgccg attgagtact agtggctcgg cgccggcggc gatcgtcgga aatgtggcgg
     2101 gcgatgccca gctgtcgggg cctgcgaggt cgcggaggcc tcgtgccgat cgtcttcatc
     2161 atcgagggcg acacgttgta ctccgccacc gacgccggcc c
//
LOCUS       DASPWO010000225         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_455056, whole genome shotgun sequence.
ACCESSION   DASPWO010000225 DASPWO010000000
VERSION     DASPWO010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455056"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..401
                     /locus_tag="VEO00_04985"
     CDS             129..401
                     /locus_tag="VEO00_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57388.1"
                     /translation="MSPRDSFQEFEATSLFCPRCKRATRARQKLLLVLPSGNKYDYVC
                     AECGTAVGAKTDNDSTSFYRTAPPPAAAPRPGAVPPRGLPGPRRRP"
     gene            665..>2642
                     /locus_tag="VEO00_04990"
     CDS             665..>2642
                     /locus_tag="VEO00_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYU57389.1"
                     /translation="MASLIGRSVLRKEDARLVTGHGCFVDDLRLPDMAHLAIVRSPHA
                     HARVRAIDGRAAHAVPGVLAVLSLADLPECAAPVPSLVPSPKFRSYVQPVLAGPRVRC
                     AGEAVAVVVAADRYVAEDAAAAVLVEYEVLPAAATPAQALAANAPRVHEDWPDNLAGT
                     SRSAVGDVDAAWSGAEVVVGARLAYPRLAGMPIEPRGVAAAVDVTGLLTVWTSTQVPF
                     AVRSGIAVALGMPEERVRVIAPDVGGGFGVKGHAYPEDILIPAVARRLGRPVKWTETR
                     REHLLAAAHDRDQVHDARIGVRRSGEIVALETRFTRDHGAYPTLGDAITLNTINHLPG
                     PYRVPAYRATGDNVVTHKTFAAAYRGAGRPEAAFVLDRLLDRAARAIGMDPADLRRHN
                     LVRPDEMPFASGLSYRDGVPIAYDPANYVAGFDRLLSRFDYDGWRAEQKRRRGSGTPL
                     GLGLSAYVEGTGLGPFEGAEVRVDPGGTVFLHLGVSSQGQAHETTLAQIAADRLGVGV
                     EDVVVMGGDTSLVGFGMGTIASRVAAVAGPAIDRTAGEVARKTRLVAADLLECAPDDV
                     VLAGGRVHVAGLPDRGFPLARVAREAVRSRRLATEGGPGLTACGFFYPDTVTWAFGAH
                     ACALEVDVETGAVRLLRYVAVHDCGRPINPMIVEG"
BASE COUNT          332 a          993 c          935 g          382 t
ORIGIN      
        1 catcgcgaag ctggcaactc ggttggcctg aacgccgtac tttcgtggtt cccgttttcc
       61 cggcgcgtca atcaggattc ttcttgctcg gctcgccttc ggctgcgcct cgcactacaa
      121 tttcgggcat gagcccgcga gacagcttcc aggagttcga ggccaccagc ctcttctgcc
      181 cccgctgcaa gcgggcgacc cgcgcccgcc agaagctcct gctcgtcctg ccgagtggca
      241 acaagtacga ctacgtgtgc gccgagtgcg gcaccgccgt cggggccaag accgacaacg
      301 acagcacgag cttttaccgc actgcccctc cgcccgcggc cgctccgcgc ccaggagcgg
      361 ttccgccccg cggcctgccc ggtccgcgcc ggcggcccta aatacgttcg gagggggcct
      421 cgacggcgtg tcgatccagg tcggggggag cgacgccggg ttccggattt cgctcccctc
      481 gggactggcg gggcccagcc caccggagtc ccgcgggtcg cccttcgccc gacacccccc
      541 accggtctta cgcggcggcc gtcgtcccag agagttgcgc gggcgaagcc cgcgctcgaa
      601 gggcgtgaaa ccgtgagaat gcccgacaca caccctggct gggacacgaa cgtcgagcat
      661 gtccgtggct agcctgatcg gccgttcggt tcttcgcaag gaggacgcac ggctcgtgac
      721 cgggcacggg tgcttcgtcg acgacctgcg gctgccggac atggcgcatc tcgcgatcgt
      781 ccggagcccc catgcgcacg cgcgggtgag ggccatcgat ggccgggccg cgcacgccgt
      841 cccgggcgtg ctcgccgtcc tctcgctcgc cgatctcccc gagtgcgcgg cccccgttcc
      901 cagcctggtg ccgtccccga agtttcgctc gtacgtccag cccgtgctcg cgggcccgcg
      961 cgtgcgctgc gcaggcgagg ccgtggccgt cgtcgtcgcg gccgatcgct acgtggccga
     1021 ggacgccgcg gccgccgtcc tcgtcgagta cgaggtcctg cccgccgccg ccacgcccgc
     1081 ccaggcgctg gccgccaacg cgccgcgcgt ccacgaggac tggccggaca acctcgcggg
     1141 gacgtcgcgg agcgcggtcg gagacgtcga cgcggcgtgg agtggagccg aggtcgtcgt
     1201 cggcgcgcgg ctggcgtatc cccgactggc gggcatgccg atcgagccgc gtggcgtggc
     1261 agccgccgtc gacgtcaccg ggctgctgac ggtctggacg tccacccagg tcccgttcgc
     1321 ggtccgcagc ggcatcgccg tggcgctggg gatgcccgag gagcgcgtgc gcgtgatcgc
     1381 gcccgacgtc ggcggcgggt tcggcgtcaa gggccacgcc taccccgagg acatcctgat
     1441 cccggccgtg gcgcgccggc tcgggcggcc cgtgaagtgg accgagacac gccgggagca
     1501 cttgctcgcc gcggcccacg accgcgatca ggtgcacgac gcgcggatcg gcgtgcgccg
     1561 ctcgggcgag atcgtcgcgc tggagacccg gttcacgcgc gatcacggcg cctacccgac
     1621 gctcggcgac gcgatcacgc tgaacacgat caatcacctg cccggcccat accgcgtgcc
     1681 cgcgtatcgg gccaccggcg acaacgtggt cacgcacaag acgttcgccg cggcgtaccg
     1741 tggagcaggc cggccggagg cggcgttcgt gctcgaccga ctcctcgatc gcgccgcgcg
     1801 agccatcggg atggatcccg cggacctgcg ccggcacaac ctcgtccgcc ccgacgagat
     1861 gccgttcgcc tccgggctct cctatcgcga cggcgtgccc atcgcctacg atccggcgaa
     1921 ctacgtcgcc ggcttcgacc ggttgctctc gcggttcgac tacgacggct ggcgtgcgga
     1981 gcagaagcgg cggcggggct cgggcacgcc cctgggcctc ggtctctccg cgtacgtcga
     2041 gggcaccgga ctcggtccgt tcgagggggc cgaggtccgc gtggatcccg gcggcacggt
     2101 cttcctgcac ctcggcgtgt cgagccaggg ccaggcgcac gagaccacgc tggcccagat
     2161 cgcggccgac cgcctcggcg tcggggtcga ggacgtcgtg gtgatgggcg gcgacacgtc
     2221 gctcgtcggc ttcggcatgg ggacgatcgc cagtcgcgtg gctgccgtcg ccggccccgc
     2281 catcgacagg acggccggag aggtcgcccg caagacacgg ctcgtggccg ccgacctcct
     2341 cgagtgcgcg ccagacgacg tcgtgctggc cggcggccgc gtacacgtcg ccgggcttcc
     2401 ggaccggggc ttcccgctcg cccgcgtcgc gcgcgaggcc gtgcggagtc ggcggctggc
     2461 caccgagggc ggcccgggcc tgaccgcctg tggcttcttc tatcccgaca ccgtgacctg
     2521 ggcgttcggc gcgcacgcct gcgccctcga ggtcgacgtg gagacgggcg ctgtccggct
     2581 ccttcgctac gtcgccgtcc acgactgcgg gcgcccgatc aacccgatga tcgtcgaggg
     2641 ac
//
LOCUS       DASPWO010000226        12063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_882253, whole genome shotgun sequence.
ACCESSION   DASPWO010000226 DASPWO010000000
VERSION     DASPWO010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12063
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882253"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2502)
                     /locus_tag="VEO00_04995"
     CDS             complement(<1..2502)
                     /locus_tag="VEO00_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYU57390.1"
                     /translation="MASMTGAYDAVAEIGVGLLNAVLGAVHENQEPGYPAMPHAVSGW
                     IDDTYRGGSDPLPEAERTGFRARVEVQVSTPIVSLPVDGLVVPPLILAAAGGAASRSE
                     PERRGPGPETAWLGRDIGVAWPWRGPQVATRVRVRAWVRDGEGPPLPEFIDGNLTVST
                     GIRRSDVTGVGTFLTMDRSSGPTVAFEPAPGTQITDEQRQLVASIVRNVIRANLAPET
                     FRVTVPPEVRHFDFVLRPEARRPCVQLLLNLTDRAVGPGDRGSAAPGILPDGADFAIA
                     VSRDFLIAQFASRLFQGLPASYSYSTWGVGATVRPDWAGATFELEPGRVVFKLRGDGD
                     ISWFGVDDHFTFAIQQAFGLVLVGGRIDVVADGDPVVDLTDVAVGGGYLEGKARDTIR
                     AERDAALAQAGPQIRRALDLGRHLEQILGGFHPRPAGVAITGVQLGPDGVLASGTVAL
                     AASGPVVVAHDDRGGYRDALESWIPGGTVDRFTWERSRFPRGTPEVHVEEHRFVTEGM
                     TIAIMGSVCLMVEGTRVGPGGSILPVSLRTCLPFIPVIGPVNDLPTDVPRPVLPFRET
                     LADGTARTIGHYDPWGPGGAGGDTVLLLLFGSEEGEDPAELLREARKRAKKGDAAIVA
                     VTIAPGDRAGAAREPAADIVVAEDPQGPWAEAFGVSTTPAAVLVGPGGGGVWRGTGAK
                     EVTAARLARAIDEHAVPDGRMAMRPIRLPVRVGQRVPDASLRLAGGGELSLRRLRGRP
                     IAITFWSSRSAPSLEQLDVLRDVADRSGGGGPLVVAVGDGESQERATEVAKERRLPYL
                     VLPDPDRLVSSAFGVWCWPATVWIRPDLRVEAIDVG"
     gene            complement(2513..2968)
                     /locus_tag="VEO00_05000"
     CDS             complement(2513..2968)
                     /locus_tag="VEO00_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57391.1"
                     /translation="MDWFVLLTPLLALAVVLLLGFTGCEFEHGTLPTLPKLLLRTKVP
                     TAITVTVCRFRYLEPGATVEVPEDGVPRVEGSDTFYEFTLTGPLPGTWTINCRLGAKN
                     PAGQTVERNRMGTFALDPVATPNVVSNWETVGMPGDPGYDLLWIGRDPP"
     gene            complement(3074..3532)
                     /locus_tag="VEO00_05005"
     CDS             complement(3074..3532)
                     /locus_tag="VEO00_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57392.1"
                     /translation="MDWFVLLTPLLALAIVLLVGFTGCDRIFRLDPLPTGTLLLRTRV
                     PTAITVTVCKFRYRPPGATSDLVSDGVPRAEGSDTFYEFSVAGPPPGTWSINCRLGAK
                     NAAGQTVERNRMGTFALDPVATPAVVANWETVGMPGDPNYDLLWIGRDPP"
     gene            complement(3553..4284)
                     /locus_tag="VEO00_05010"
     CDS             complement(3553..4284)
                     /locus_tag="VEO00_05010"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HYU57393.1"
                     /translation="MRPVLFRWRGRPIPSYPAMLYLGIVAALAAGNALANARGLSGAR
                     VYLASVLLAFPALLGGRLAYVAAHWSAYRIDPHRIWRRSDGGQVTYGALLAVPVSVPL
                     LAALGLPFALFWDIGACSLLAAMVFTRVGCVLNGCCAGRPTESRFGLTLAGVHGVRAR
                     RVPTQLLEAGVAAVLLASSAVLVLRHAPPGTVFAGALAAYALGRLVLDALRERQRRVA
                     GVPALRAVAAVLLAVALWSLLPRVL"
     gene            complement(4319..4756)
                     /locus_tag="VEO00_05015"
     CDS             complement(4319..4756)
                     /locus_tag="VEO00_05015"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HYU57394.1"
                     /translation="MPALGSWLLPLSLAVVLVVDQALKTVALAGLRDGGVVGLGAVLR
                     IRPVVARGGIAARLGAHRAALAAVWLGCLLVILLAAPRAGLFDTSLARVALGAALGGA
                     AGNLLDLFRRGGVVDYVALPFWPAFNLADVAIVAGVTVAFIER"
     gene            complement(4811..5278)
                     /locus_tag="VEO00_05020"
     CDS             complement(4811..5278)
                     /locus_tag="VEO00_05020"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYU57395.1"
                     /translation="MSYELRVERLIDAPPEVVFDTLVDPDAQKEIFSGQVEGWSLLES
                     DIDLRVGGTWTTVIGPAEGEPDRLTNVFTAVERPRRLAYRVSMYVGDWGRTIDSEVTI
                     TFDDRDGKTLVTIVQGGFETEADRDAYLSGAPGFLDALQKAVASRVAGRHDNG"
     gene            complement(5275..5628)
                     /locus_tag="VEO00_05025"
     CDS             complement(5275..5628)
                     /locus_tag="VEO00_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYU57396.1"
                     /translation="MVKSQLLDRTFAALSDPTRRDILEQLSAGPASISELARPSGISL
                     PGVMKHVRVLEAASLVTTEKHGRVRQCRLGPGGMDDATRWIESHRRQWERRLDRLETI
                     VARKTRPAMRKGDLP"
     gene            5782..7152
                     /locus_tag="VEO00_05030"
     CDS             5782..7152
                     /locus_tag="VEO00_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018742381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYU57397.1"
                     /translation="MPTSPTLSIPDLRAAVDGRVIAPDDEEYDRARTPFYGGFDRRPA
                     AIVRVANANDVATVISVARDTGLELAVRSGGHSIPAHSVSDGGIVLDLADMRALEIDA
                     EGRTAWAETGLTTGAYSNATAAHGLATGFGDTGTVGIGGLTLGGGVGLLVRKYGLTID
                     DLLAADVVTADGRLLRADDETNPDLFWALRGGGGNFGVATRFRFRLHELSAIVGGMLF
                     LPATPEVVAGFIAEAVAAPEELSTIANVMVAPPMPFVPQEAHGKLVVMALMVHAGGGE
                     AGERAVAPFRALATPIADMLRPMTYPEMFEPAQEDYHPIATGRTMFVDHVDLPVAETI
                     FEQLESSPATMKVAQLRVLGGAMARVPADATAYAHRASRIMVNVAALSERPEEAEGYE
                     PWVEGFAAILRQGDGGAYVNFLDDEGESRIRAAYPGKTWDRLREIKARYDPANLFRLN
                     QNIRPA"
     gene            7550..8194
                     /locus_tag="VEO00_05035"
     CDS             7550..8194
                     /locus_tag="VEO00_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYU57398.1"
                     /translation="MDRALVRLQEAGARAEGAVGDADPFLAIEETLRSQRFDEIIIST
                     FPPRLSAWLHSDLVRQVASTFNIPVTHVVAPPERATRETALMRVPLFTGLSKRRIRAL
                     ARASMVNLYRKGETIVRQGSAGSELFVILDGGVKVVRGGRTVARLRPGEVFGEISLLD
                     GGPRTADVIAEMTTRGVCLSGADFRAVLEADPVIATRILQEAGRRLRKLAQSAT"
     gene            8400..10544
                     /locus_tag="VEO00_05040"
     CDS             8400..10544
                     /locus_tag="VEO00_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYU57399.1"
                     /translation="MDRPSQAPGSTERLVVVPHAHRQRRPTGAPPPLPKKIGLTGKLW
                     VAAVLVVVVSGAAWLDVTTGPLDHFDAVIIRFVTAARTPWLDSFARFLDSVGSRLWLA
                     IWGLLTVAVTVAFRRWRHLLVFIVSLATLEIVFPFLYRTAARPRPYSVTAIGHWEGFS
                     SPSQSMAALTAVLMGFIYMVVVAGRPRWNAKLAIMAVLAGISLNRIYLGIDHPTDLAF
                     AVILGVAIPVALFRAFTPSDIFPVRYGPRGKTAHLDVGGRRGEAIRRAMQDQLGFTIL
                     EMKLVGLEGSGGSTPLKLRVSDEQGVERSVFAKLYAKNHVRADRWYKLGRMMMYGRLE
                     DETPFQTVRRFVEYEDYTLRLLGEYGFPTPAPLGVVEITPEVEYLIAMEFFDGGEEIG
                     DVDIDEDVIDEGLRMIRRMWDVGLAHRDIKPANLMVQDGHLRLIDVFFVQVRPSPWRQ
                     AVDLGNMMLVLALRSDAQIVYERALGYFTPEELSEAFAATRGVASPTQLRTFLKRDGR
                     DLLEQFRRLAPSRPPVRLQLWSVRRVGMIILTVVAVVLALVYSLSLFVPSRGHVLTPS
                     CETNRTMILMAQAVPSAEQLPCIRSLPLGWSVSGVTIARGRATFELLDTGEDRGGGGY
                     IQLQIAQGGESPAVDVTLTPTCPATSEDPTIQTIEVEGGCVTYHSVPAGGEPVPSFDP
                     DAELSFVSRSQLVTFVEQDEDLVLCGAGAPCP"
     gene            10718..11407
                     /locus_tag="VEO00_05045"
                     /pseudo
     CDS             10718..11407
                     /locus_tag="VEO00_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434138.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
     gene            11407..11730
                     /locus_tag="VEO00_05050"
     CDS             11407..11730
                     /locus_tag="VEO00_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HYU57400.1"
                     /translation="MTADAGTRRYGTFSYLPPLTKEEIGKQIDYILGQSLVPAIEFTP
                     DPGPRNVFWSMWKLPLFDVETAAAILQEIEACAREHPDSFIKLNGYDPDRQAQAASFV
                     VRRPD"
BASE COUNT         1872 a         4304 c         4141 g         1746 t
ORIGIN      
        1 gccgacgtcg atcgcctcca cgcgcaggtc cggacggatc cagacggtcg cgggccagca
       61 ccacacgccg aacgcgctcg agacgaggcg gtccgggtcg ggcaggacga ggtatggcag
      121 ccgccgttcc ttcgccacct cggtggcccg ctcctggctc tctccgtcgc cgacggccac
      181 aacgaggggg ccacctcccc cggagcggtc cgcgacgtcg cgcaggacgt cgagctgctc
      241 caggctcggt gccgatcgcg agctccagaa cgtgatcgcg atgggccggc cccgaagccg
      301 ccggaggctc agctccccgc cgccggccag ccggagcgag gcgtctggca cgcgctgccc
      361 gacacgcacg ggaagccgga tcggccgcat ggccatccgt ccatcgggca cggcgtgctc
      421 gtcgatcgcg cgcgccagcc tggcggcggt cacctccttc gcgcccgtcc cgcgccagac
      481 gccgccgccg ccggggccga ccaggacggc ggcgggggtc gtggacaccc cgaacgcctc
      541 cgcccacggg ccctgcgggt cctcggccac gacgatgtcc gccgcgggct cgcgagccgc
      601 cccggcacgg tcgccaggag cgatcgtgac ggcgacgatc gccgcatctc ccttcttcgc
      661 gcgcttccgc gcctcccgca ggagctccgc cgggtcctcg ccctcctccg acccgaacag
      721 gagcaacagg acggtgtcgc cgcccgcccc tcccggtccc cacgggtcgt agtgaccgat
      781 cgtccgcgcc gtcccgtcgg ccagcgtctc ccggaagggc aataccgggc ggggaacatc
      841 tgtcggcagg tcgttcaccg gcccgatcac cgggatgaac gggaggcacg tccgcaggct
      901 gaccgggagg atggaccctc ccgggccgac ccgggtcccc tccaccatga ggcagacgct
      961 gcccatgatc gcgatggtca tgccctcggt gacgaagcgg tgctcctcca cgtggacctc
     1021 cggcgtgccg cgcgggaacc gcgaccgctc ccacgtgaac cggtccaccg tcccacccgg
     1081 gatccagctc tcgagcgcgt cgcgatatcc gccgcgatcg tcgtgggcga cgacgaccgg
     1141 tccggaggcc gccagggcca cggtccccga cgccaggacg ccgtccggcc cgagctgcac
     1201 gccggtgatc gccactcccg cgggacgggg gtggaagccc ccgaggatct gctcgagatg
     1261 gcgcccgagg tcgagtgccc gacggatctg cggccccgcc tgcgccagcg cggcgtcgcg
     1321 ttcggccctg atcgtgtccc gggccttgcc ctccaggtac ccgcccccca cggcgacgtc
     1381 tgtcaggtcg accaccggat cgccgtcggc caccacgtcg atcctcccac cgacgaggac
     1441 cagtccgaac gcctgttgga tggcgaacgt gaagtgatcg tccacaccga accacgagat
     1501 gtccccgtcg ccgcggagct tgaagaccac gcgccctggc tccagctcga acgtcgcgcc
     1561 cgcccaatcg ggacgcacgg tcgcaccgac gccccacgtc gagtacgagt acgaggccgg
     1621 cagcccctgg aacagccgcg acgcgaactg ggcgatcagg aaatcgcggc tcaccgcgat
     1681 ggcgaagtcg gctccgtccg gcaggatccc cggagcggcg ctcccgcggt cgcccggccc
     1741 gaccgcccgg tccgtcaggt tgagcagcag ctgcacgcac ggccggcggg cctccggccg
     1801 caggacgaag tcgaagtggc ggacctccgg cggaacggtg acccggaacg tctcgggagc
     1861 gaggttcgcc cggatcacgt tgcgcacgat gctcgccacg agctgccgtt gctcgtcggt
     1921 gatctgcgtt ccgggcgccg gctcgaacgc cacggtcggc ccggacgagc ggtccatcgt
     1981 caggaacgtc ccgaccccgg tgacgtccga tcgacggatg cccgtgctga ccgtgaggtt
     2041 gccgtcgatg aactcgggga gcggtggccc ctcgccgtcc cgaacccacg cccggacgcg
     2101 gacccgggtc gcgacctgcg gcccccgcca tggccaagcg accccgatgt ctcggccgag
     2161 ccacgcggtc tccggccccg gcccgcgtct ctccggctcc gagcgcgacg ccgcaccccc
     2221 cgcggcggcg aggatcagtg gcgggaccac gaggccgtcc accggcagcg acacgatcgg
     2281 cgtggacacc tggacctcga cgcgagcgcg gaatcccgtg cgctccgcct ccggaagcgg
     2341 gtcgctgccg ccccggtacg tgtcgtcgat ccacccgctc acggcgtggg gcatcgcagg
     2401 gtacccgggc tcctggttct cgtggacggc cccgagcacg gcgttgagca gtcccacgcc
     2461 gatctcggcg acagcatcgt acgcaccggt catgctagcc atgcgctgcc tcctatggcg
     2521 gatcgcgccc gatccatagc aggtcgtagc ccgggtcgcc gggcatcccg acagtctccc
     2581 agttcgacac gacgttcggc gtggccaccg ggtccagggc gaacgtcccc atccggttcc
     2641 gctccaccgt ctggccggcg gggttctttg ctccgaggcg gcagttgatc gtccaggttc
     2701 cgggaagcgg tccggtgagc gtgaactcgt agaacgtgtc gctcccctcg acgcgaggga
     2761 cgccgtcttc cgggacctcg accgtcgccc cgggctccag gtaccggaac ctgcagaccg
     2821 tgaccgtgat ggccgtcggg accttggtcc gcagcaggag cttcggcagc gtcggcagcg
     2881 tcccgtgctc aaattcgcag ccggtgaagc cgaggagcag gacgacggcg agcgccagga
     2941 gcggcgtgag gagcacgaac cagtccattc cccctcccct cccccctaga gcgccctcgg
     3001 ggccagcgag gggccagtgc gaacggcgag gagcaccgcc accgcgcgcc tcagcctggc
     3061 catgcgctgc ctcctatggc ggatcgcgcc cgatccacag caggtcgtag ttcgggtcgc
     3121 cgggcatccc gaccgtctcc cagttcgcca cgacggccgg cgtcgccacc gggtccaggg
     3181 cgaacgtccc catccggttc cgctcgaccg tctggccggc ggcgttcttt gcgccgagcc
     3241 ggcagttgat cgaccaggtt ccgggcggcg gtcccgcgac gctgaactcg tagaacgtgt
     3301 cgctcccctc ggcccgaggg acgccgtcgg acacgaggtc gctcgtcgcc ccgggaggcc
     3361 ggtaccggaa cttgcagacc gtgaccgtga tggccgtcgg gaccctggtc cgcagcagga
     3421 gcgtacccgt cggcagcggg tccagcctga aaatcctgtc gcagccggtg aagccgacga
     3481 gcaggacgat ggcgagcgcc aggagtggcg tcaggagcac gaaccagtcc atgcctctcg
     3541 tcccctcctc cgctagagca cccgagggag caacgaccac agcgccacgg cgaggagcac
     3601 cgccgccacc gcccgcagcg ccgggacgcc ggccacgcgg cgctgccgct cgcgcagcgc
     3661 gtccaggacc agccgtccaa gcgcgtacgc tgccaacgcg cccgcgaaca ccgtgccggg
     3721 gggcgcgtgc cgcaggacca ggacggcgga gctcgccagc aggacggccg cgacccccgc
     3781 ctccagcagc tgcgtgggca cccgccgcgc gcggacgccg tggacgccgg ccagcgtcag
     3841 gccgaagcga ctctcggtag ggcggcccgc gcagcacccg ttcagcacgc agcccacccg
     3901 ggtgaagacc atcgccgcca ggagcgagca cgccccaatg tcccagaaca gcgcgaacgg
     3961 cagcccgagc gccgccagca gcggcaccga gacgggcacc gccagcaggg cgccgtacgt
     4021 gacctggccc ccgtctgacc ttcgccagat ccggtgtggg tcgatccggt aggcactcca
     4081 gtgggccgcc acgtacgcca ggcggccacc gaggagcgcc gggaaggcca gcagcacgct
     4141 cgccaggtag acccgcgcgc cgctcagccc gcgcgcgttc gccagggcgt tgccggccgc
     4201 cagggcggcc acgatgccca gatagagcat cgccgggtag ctcgggatcg gccggccccg
     4261 ccagcggaac aggaccggtc gcacctagcg gactccccgg gacggccgcg gagaggcgtc
     4321 agcgttcgat gaaggcaacg gtcacccccg caacgatcgc gacgtccgcc aggttgaagg
     4381 ccggccagaa cgggagggcg acgtagtcga ccacgccgcc ccgccggaag aggtcgagca
     4441 ggttgcccgc tgcgccaccg agggcggcgc ccagtgccac ccgggcgagc gacgtgtcga
     4501 agagaccggc acggggcgcg gccaggagga tcaccagcag gcaccccagc cagacagccg
     4561 ccagggcggc gcggtgcgct ccgagccggg cggcgatgcc cccgcgggcc acgaccggcc
     4621 ggatccggag gaccgcaccg agcccgacga ccccgccgtc ccggaggccg gccaacgcga
     4681 cggtcttcag cgcctggtcg acgacgagga cgacggcgag cgagagcggg agcagccaac
     4741 tccccagcgc cggcatcgtg ccgggatcat accgagagcc cggatcgcga aggaaatccc
     4801 gatcgcgtcc tcagccgttg tcgtgccgtc ccgcgaccct ggacgccacg gccttctgca
     4861 gcgcgtcgag gaacccgggg gcgccgctca ggtacgcgtc gcggtccgcc tcggtctcga
     4921 acccgccctg cacgatcgtc acgagcgtct tcccgtcccg atcgtcgaac gtgatggtca
     4981 cctcgctgtc gatcgtccgt ccccaatcac cgacgtacat ggacacgcga tacgccagtc
     5041 gacgcggccg ttcgaccgcg gtgaacacgt tcgtcaggcg gtcgggctcc ccctccgccg
     5101 gccctatcac ggtggtccac gtaccgccaa cgcggagatc gatgtcggac tccagcaggc
     5161 tccatccctc cacctggccg gagaagatct ccttctgggc gtccgggtcg acgagcgtgt
     5221 cgaagacgac ctctggtgga gcgtcgatca ggcgctcgac cctcagttcg tagctcacgg
     5281 cagatctccc ttcctcattg ccgggcgcgt cttccgcgcg acgatcgtct cgagacggtc
     5341 cagccggcgt tcccattgcc gccggtgcga ctcgatccat cgcgtcgcgt cgtccatgcc
     5401 cccggggccg agtcgacact ggcggacgcg cccgtgcttc tccgtggtca ccaggctcgc
     5461 ggcctcgagg acacgcacgt gcttcatcac tcccggcagt gagatcccgg agggccgcgc
     5521 cagctcgctg atcgaggcgg gcccggccga gagctgctcg aggatgtcgc gccgcgtcgg
     5581 gtccgacaac gcggcgaacg tccggtcgag cagttgagac ttaaccatac agtttactat
     5641 catctcgaca ccgcgaacgt caactgctcg ggagcgcgta cccgaggact cctgcgggct
     5701 gccctagggt ggaatgctcc gggcgggccg accgccgacg atacgagaac cgaacctcac
     5761 ggaccgatcg gagggacctc gatgccaaca tcccccacgc tctcgatccc cgatcttcgc
     5821 gccgcggtcg acggccgggt catcgcgccg gacgacgagg agtacgaccg ggcgcggacc
     5881 ccgttctacg gcgggttcga ccgccggccg gcggcgatcg tccgggtcgc gaacgcgaac
     5941 gacgtcgcga ccgtcatctc ggtggcacgg gacacgggcc tggagctcgc cgtgcgcagc
     6001 ggcggacaca gcatccccgc gcacagcgtc tccgacggcg ggatcgtgct cgacctcgcg
     6061 gacatgcggg cgctggagat cgacgccgag ggacgcacgg cgtgggccga gaccggcctg
     6121 acgaccggcg cgtactcgaa cgcgacggcg gcccacgggt tggcgaccgg gttcggcgac
     6181 accggcacgg tcgggatcgg gggcctgacg ctcggcggcg gtgtcggcct cctggtccgc
     6241 aagtacggcc tgacgatcga cgacctgctg gccgccgacg tggtgactgc ggacggccgg
     6301 ctgctgcgcg ccgacgacga gaccaacccc gacctgttct gggcgctccg cggcggcggc
     6361 gggaacttcg gggtggcgac gcggttccgg ttccggctgc acgagctctc cgccatcgtc
     6421 ggcggcatgc tgttcctgcc ggcgacgccc gaggtcgtcg cgggcttcat cgccgaggcg
     6481 gtggccgcgc cggaggagct gtcgacgatc gccaacgtca tggtggcgcc gccgatgccg
     6541 ttcgtgcccc aggaggccca cggcaagctg gtggtgatgg ccctgatggt gcacgccggc
     6601 ggcggggagg ccggcgagcg cgcggtggcg ccgttccggg cgctggccac gccgatcgcg
     6661 gacatgctcc gtccgatgac ctacccggag atgttcgagc cggcccagga ggactaccac
     6721 ccgatcgcca ccgggcgaac gatgttcgtc gaccacgtcg acctgcccgt ggcggagacg
     6781 atcttcgagc agctggagtc ctcgcccgcg acgatgaagg tggcccagct ccgggtgctc
     6841 ggcggggcga tggcccgcgt gccggcggac gcgacggcgt acgcgcatcg cgccagccgg
     6901 atcatggtga acgtcgccgc gctctccgag cggcccgagg aagccgaggg gtacgaaccg
     6961 tgggtcgaag gcttcgccgc gatcctgcgc caaggcgacg gcggcgcgta cgtcaacttc
     7021 ctcgacgacg agggcgaatc acggatccgc gccgcctacc cgggcaagac ctgggaccgt
     7081 ctgcgggaga tcaaggcccg ctacgacccc gccaacctgt tccgcctgaa ccagaacatc
     7141 cggccggcgt agaatcggga cgcgacctca cgtcgtggcc ttgcctggcg tcccgtagga
     7201 ctggaggcgg gggggactct gtgtgttcgt tcagttccac gtggcagcgg gacaatgatc
     7261 tggccgggac cgaggcgcct gtggacccga gcgggcggcc tcgatatgct aggtgagcgg
     7321 atacagagtt gtccccgctc ggtccagaaa gacgtctcat ccgggaaccg acatgcagcg
     7381 ctatttggtg gttgccaacc agaccctcat tagcgacgcc ctccttggcg aggtcatcga
     7441 ccgcctgaag agagggccat gtcgcttcca tgtcctggtg cctgccactc cccccgctga
     7501 gggctggacc tggacggacg gagaagcaag aggactggcc gaggagcggt tggaccgggc
     7561 cctcgtgcgg ctccaagagg ccggcgcccg ggcggaaggc gctgtggggg atgctgaccc
     7621 gttcctcgcc atcgaggaaa ccctcaggag ccagcggttc gacgagatca tcatctcgac
     7681 gttcccgccc cgactgtctg catggctaca ttccgacctc gtccgccaag tcgcttccac
     7741 gttcaacatc cccgtgacgc acgtcgtcgc tccgccggag cgggcgacga gggagactgc
     7801 tctcatgcgg gtaccactct tcacggggct ctccaagcgc cggatccgcg cgctcgccag
     7861 agcgagcatg gtcaacctgt acagaaaggg agagaccatc gttcgacagg gctctgcagg
     7921 atccgagttg ttcgtgatcc tcgacggagg agtgaaggtg gtccgcggag gacgtaccgt
     7981 ggcccggctg cgcccggggg aagtgttcgg ggagatctcc cttctcgacg gcggccctcg
     8041 caccgccgac gtcatcgccg agatgaccac tcgaggcgtc tgcctctcgg gcgcggattt
     8101 ccgtgccgtt ctcgaagccg atcctgtgat cgccacgagg atcctgcagg aggccgggag
     8161 acgattgcgc aaactggctc agtccgcgac ctgaaccatc tcggtttgtc cgctgcacac
     8221 cccggccgac actgggcaca accagcaact cgaggccgct cgctccggtc gatctcagta
     8281 ttggtgcagg gctcctttag ccccttcgga cgagattcgt tacccgcggc tgttcgacag
     8341 gttgagcggg cagccgggct cgggattatc gttcgaggat ctcctgacag ggtggccgtg
     8401 tggacagacc gtcgcaggcg ccgggatcga cggaacgcct cgtggtcgtg ccgcacgccc
     8461 accgacaacg gcgtccgacg ggagcgccgc cgccccttcc gaagaagatc ggcctgaccg
     8521 gcaagctctg ggtggcggcc gtcctggtcg tcgtggtctc gggggccgcc tggctggacg
     8581 tcacgaccgg ccccctcgac cacttcgacg ccgtgatcat ccggttcgtg acggcggcgc
     8641 gcacgccctg gctcgactcg ttcgccaggt tcctcgactc ggttggatcc cggttgtggc
     8701 tggccatctg gggcctcctc acggtggcgg tcaccgtcgc cttccgtcga tggaggcatc
     8761 tgctggtctt catcgtgagc ctcgcgacgc tcgagatcgt cttccccttc ctgtaccgca
     8821 cggctgctcg gccgcgtccc tacagcgtca cggcgatcgg ccattgggag gggttctcat
     8881 caccctcaca gtcgatggcg gctctcaccg ccgtgctcat ggggttcatc tacatggtgg
     8941 tggtggccgg ccgtccccga tggaacgcga agctcgcgat catggccgtc ctcgcgggga
     9001 tctcgctcaa ccggatctac ctcggcatcg accaccccac cgatctggcg ttcgcggtca
     9061 tcctcggcgt ggcgatcccg gtcgcgctgt tccgcgcctt caccccgagc gacatcttcc
     9121 ccgtgcgtta cgggccccgc ggcaagacgg cgcacctgga cgtgggcggc cggcggggtg
     9181 aggcgatcag gcgcgccatg caggaccaac tcgggttcac gatccttgag atgaagttgg
     9241 ttgggctcga gggctccggg ggatcgacgc cgctgaagct ccgagtctcc gacgagcagg
     9301 gggtcgagcg gtcggtgttc gcgaagctgt acgcgaagaa ccacgtacgc gccgaccgct
     9361 ggtacaagct cggccggatg atgatgtacg ggcggctcga ggacgaaacg ccgttccaga
     9421 cggttcgccg gttcgtcgag tacgaggact acacgctccg actcttgggc gagtacgggt
     9481 tcccgacgcc ggcgccgctc ggcgtcgtcg agatcactcc cgaggtcgaa tacctcatcg
     9541 cgatggagtt cttcgacggg ggcgaggaga tcggagacgt cgacatcgac gaggacgtga
     9601 tcgacgaagg actccggatg atccggcgca tgtgggacgt gggactggcc caccgcgaca
     9661 tcaagccggc gaacctgatg gtgcaggacg ggcatctgcg gctgatcgac gtcttcttcg
     9721 tccaggttcg gccctcgccg tggcggcagg cggtggacct cgggaacatg atgctagtgc
     9781 tcgcgctccg ctcggacgcc cagatcgtgt acgagagggc cctcgggtac ttcacacccg
     9841 aggagctctc cgaggcgttc gccgcgacgc gtggcgtggc gagccccacg cagctgcgca
     9901 ccttcctgaa gcgggacgga cgcgacctgc tggagcagtt ccggaggctc gctccatcgc
     9961 gaccgcccgt caggcttcag ctgtggagcg tcaggcgggt ggggatgatc atcctcacgg
    10021 tggtcgctgt cgtcctcgcg ctggtctaca gcctatcgct gttcgttccg agccgggggc
    10081 acgtgctgac cccgtcctgc gaaacgaacc ggacgatgat actgatggcc caggccgtgc
    10141 cctcagcaga acagctgcct tgcattcggt cgctcccgtt ggggtggagc gtttccggag
    10201 tcacgatcgc gcgcgggcgt gcgacgttcg agctgttgga tacgggcgag gacaggggcg
    10261 gcggcggata catccagctg caaatagccc agggcggcga gagtccggcc gtcgacgtga
    10321 ccttgacacc gacctgcccc gcgacgagcg aggatccgac gatccaaacg atcgaggtcg
    10381 agggcggatg cgttacctac cattcggttc cggccggcgg cgagccggtg ccatcgttcg
    10441 accccgacgc cgagctgtcg ttcgtctcgc ggtcccagct cgtgacgttc gtcgagcaag
    10501 acgaggatct ggtcctctgc ggcgccggcg cgccgtgccc gtgagagtcc gccgcccgtc
    10561 gcgtcggtta gtttcgctcg aggtcgtgca ggagcactgg gtcgaatggc ccgacgccgt
    10621 ttaagacaaa gtcggaaaca tcctcctgtc gcggatcatg ggcgccgagg tgcggctcga
    10681 cccggctggc caccaccatg tcgccggcgc ccgaccgcgg gccgagttcg caaaggagct
    10741 gggcagcccg atcatcatgc tggacttcct caccgccggc ttcacggcac acacctcctt
    10801 gtcgaagtgg tgccgcgcga acgggatgct gctgcactgc caccgtgcca tgcatgccgt
    10861 gatcgatcga cagcgcaatc acgggatcca ctggcgggtg ctggccaagt ggtgccggat
    10921 gatcggggcc gaccacctcc acaacggcac cgtagtcggc aagctcgagg gcgatcgcgc
    10981 cgcgaccgtg gccatcaacg acctcctgcg cgaggacttc atcgccgccg acccgtcgcg
    11041 cgggatctac ttcgaccagt cgtgggggtc gctcccgagc atgtttccgg tggcctcggg
    11101 cgggatccac gtgtggcaca tgccggacct cgtggcgatc ttcggcgacg attccatcct
    11161 gcagttcgga ggcgggacgc tcggtcaccc gtgggggaat gccgccggtg cgaccgcgaa
    11221 ccgcgtggcc ctggaggccg tggttcaggc ccggaacgag ggccggggcc tgtccgcaga
    11281 gggccccgac atcctgcgcg gggcagccaa gcactccccc gaactggggg cggcgctcga
    11341 ggtgtggcag gaggtcacgt tcgacttcga agccgtcgat gccccggacc gtgaggaggt
    11401 gaagtgatga ctgcggacgc cggtaccagg cgctacggga cgttctcgta cctgcctccg
    11461 cttacgaagg aagagatcgg gaagcagatc gactacatcc ttggccagag tctggtcccg
    11521 gcgatcgagt tcacgcccga ccccggtccg cgcaacgtgt tctggagcat gtggaagcta
    11581 cccctgttcg atgtggaaac cgcggctgcg atcctccagg agatcgaagc ctgtgcgcgc
    11641 gagcacccgg acagcttcat caagctcaac ggctacgatc cggaccggca agcccaggcg
    11701 gccagcttcg tcgtccgtcg cccggactga catggcggca tcgccgcgac gccactggcc
    11761 agccgctggg gatcctgtgg acctaggggg acgctattcg aactccatcg cgacgctgtt
    11821 gaaatcgctc gagcccaccg atccatagcg cctcggaagc cgaccctgga gtccagattg
    11881 gagatgggca acgcgaacgc gcggaccgtc ctgcgaacca gaatccccac gcggcctcgg
    11941 ctccgcatct cgagcggggg cagcgcggaa ccctaccggg gtcggtgagc gaggcttcag
    12001 cggcggggaa cgctgcgacc gcgagcgatg cgaggaagaa gagcgggaaa acgtgccgct
    12061 gat
//
LOCUS       DASPWO010000227         4855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_711488, whole genome shotgun sequence.
ACCESSION   DASPWO010000227 DASPWO010000000
VERSION     DASPWO010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711488"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VEO00_05055"
     CDS             <1..365
                     /locus_tag="VEO00_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57401.1"
                     /translation="AVDPERADELLRTWSETRLPVALEAVHCPDRNIGRCTVKTVARL
                     QGPRSEVTLVIPRRDYTQGWHRLLHDRTSRTIARAVGDLAHVDVVVVPYRLGAPKAPP
                     APAEAEAPVAEPAAPARV"
     gene            376..1035
                     /locus_tag="VEO00_05060"
     CDS             376..1035
                     /locus_tag="VEO00_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HYU57402.1"
                     /translation="MHFVIMGCGRVGAELTVQLGKGGHSVSIIDKRPQAFDRLPPGFE
                     ARTIVGLGFDRETLEEAGIKEADAFIAVSNGDNSNIVSARIAREHYHVDKVIARIYDP
                     RRADIYEKLNIPTIASVRWAARQIQFLLFHGREQLLDSLAGGSLFVLRIEVPAHLVGK
                     PVSQVGTEGQVMVVGVDRGGTGFIPTPDSAFQEGDVMDVVVARDGMETLDVQLEAPAE
                     H"
     gene            1055..1774
                     /locus_tag="VEO00_05065"
     CDS             1055..1774
                     /locus_tag="VEO00_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HYU57403.1"
                     /translation="MTLQQERTEDTGRTQRGMAVVVAGAGNVGRHLAADLSERGHRIT
                     LIEQQPEMLRRAKQDVPSSVTFILGDACEPWVLEKASCNGADVMIAATGDDEDNLVIS
                     LLAKQEYAVPRVIARVNHPKNEWMFNEQWGVDVAVSPPHLLTALVEEAVTVGDLVRLM
                     RLESGQVSLVEITLREGSPNTGRPIYELRLPLDSAVVAIVRRGHVVIPQPETTLAAGD
                     EVMAISSTASEGQLRQAITGE"
     gene            complement(2140..2568)
                     /locus_tag="VEO00_05070"
     CDS             complement(2140..2568)
                     /locus_tag="VEO00_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57404.1"
                     /translation="MFDEALARLAACGCPGPAPVGIASQSRVLWAASSGEGVGLMFDS
                     GLALDYVPDSRTPEEYGTDAAAMIADDAANGGSGFRLVTVRGVVGMGADPDAHGPGAL
                     TWVTGRYSVRMLTQMLPGRTGVQFSLDDLLSIAEQLQVPR"
     gene            complement(2618..2974)
                     /locus_tag="VEO00_05075"
     CDS             complement(2618..2974)
                     /locus_tag="VEO00_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HYU57405.1"
                     /translation="MGLLSRIFRRLARTDEEVLAEETLQWASTVPGSARMTEATPRQH
                     VKLAGVVERITVRPTQGFQSLEAVLSDGTGEVTAVWLGRTSIPGLVLGSRLVLEGVLG
                     EDRGVRRLINPSYDFA"
     gene            complement(3022..>4855)
                     /locus_tag="VEO00_05080"
     CDS             complement(3022..>4855)
                     /locus_tag="VEO00_05080"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HYU57406.1"
                     /translation="SGLRRAMKIAFRAGGVAGMFTVGLGLLGATTILLIYKSDATSVL
                     VGFGFGGALLAMFMRVGGGIFTKAADVGADLVGKVEQGIPEDDPRNAAVIADNVGDNV
                     GDCAGMAADLFESYEVTLVASLILGAAAFAGSPKGAIVGVMFPLFVRAVGVVTSIVGI
                     LAVAPRSEEEHGMRAINRGFFLSAALSAAAVFAISKVYLDSWAPAVAVLLGLVLASII
                     QVLTQYFTDTKYKPVREIAEATVTGPATTILSGFSTGLESTVWSILIIAGAVIGAFLL
                     GDTNAERLYFISLAGMGMLTTVGVVVSMDTYGPVSDNAQGIAEMSGEFKGRPAVILGG
                     LDAIGNSTKAITKGMAIATAVLAATSLFGSFEEALKGAGLNFQGLRIDHPDVLVGLLL
                     GGAVAFLFSSLAIRAVGRAAQQVVVEVRNQFRDHPGIMDFSEKPDYKKVVDICTRTSL
                     RELMTPGLLAVLAPLITGFFLKAEALGAFLAGAILTGQLLAVMFANSGGAWDNAKKYI
                     EEGHYGGKGSEQHKAAVIGDTVGDPFKDTAGPALNPLIKVMNLVALLSAPLVVSHAGD
                     TGLRIAVGVVGALILAAAIWVSKSRRSELEAELKAADAKATTVA"
BASE COUNT          793 a         1767 c         1635 g          660 t
ORIGIN      
        1 tcgccgtcga ccccgaacgg gccgacgagc tgctgcgcac ctggagcgag acgcgcctgc
       61 cggtcgccct cgaggccgtt cactgccccg accgcaacat cgggcgctgc acggtgaaga
      121 ccgtcgcccg gctgcagggc ccgcggagcg aggtgacgct cgtgatcccg cgccgcgact
      181 acacgcaggg ctggcaccgg ctgctgcacg accgcaccag ccggacgatc gcccgggcgg
      241 tgggcgatct tgcccacgtg gacgtcgtgg tggtgcccta ccgtctgggc gcccccaagg
      301 cgccgcccgc gccggcggag gccgaggccc ccgtcgccga gcccgcagct ccggcccgcg
      361 tgtaggctgt cgttgatgca cttcgtgatc atgggttgcg gccgggtcgg cgcggaactc
      421 accgtgcagc tcggcaaggg cggccacagc gtctcgatca tcgacaagcg gccccaggcc
      481 ttcgaccgcc tcccgcccgg gttcgaggcg cgcacgatcg tcggcctcgg gttcgaccgg
      541 gagaccctcg aggaggccgg gatcaaggag gccgacgcct tcatcgccgt ctcgaacggc
      601 gacaactcga acatcgtctc cgcgcggatc gcgcgcgagc actaccacgt cgacaaggtg
      661 atcgcccgga tctacgaccc gcgccgggcg gacatctacg agaagctgaa catcccgacc
      721 atcgccagcg tgcgctgggc ggcgcgccag atccagttcc tgctcttcca cggcagggag
      781 cagctgctcg actcgctcgc cggcggcagc ctgttcgtgc tgcggatcga ggtcccggcc
      841 cacctcgtcg gcaagccggt gtcgcaggtc gggaccgagg gccaggtgat ggtcgtcggg
      901 gtagaccgcg gcggcacggg cttcatcccc acgcccgaca gcgcgttcca ggagggcgac
      961 gtgatggacg tcgtcgtggc gcgcgacggg atggagaccc tggacgtcca gctcgaggcc
     1021 ccggcagagc attagcgacg ggggagggaa cggcgtgacc ctgcagcagg aacggacgga
     1081 ggacaccggc cgcacgcagc gcgggatggc ggtcgtggtc gccggcgccg gcaacgtcgg
     1141 gcggcacctg gccgccgacc tgtccgagcg cggccaccgg atcacgctga tcgagcagca
     1201 gcccgagatg ctgcggcgcg ccaagcagga cgtgccgtcg tcggtgacgt tcatcctcgg
     1261 cgacgcgtgc gagccgtggg tgctggagaa ggcgtcctgc aacggggccg acgtgatgat
     1321 cgccgccacc ggcgacgacg aggacaacct ggtgatctcg ctgctggcga agcaggagta
     1381 cgccgtcccg cgcgtcatcg cccgggtgaa ccacccgaag aacgagtgga tgttcaacga
     1441 gcagtggggc gtcgacgtgg cggtcagccc gccgcacctg ctgacagcgc tcgtcgagga
     1501 ggccgtgacg gtcggggacc tggtccggct gatgcgcctg gagtcggggc aggtttccct
     1561 ggtcgagatc acgctgcgcg agggctcgcc gaacaccggc cgcccgatct acgagctgcg
     1621 cctcccgctc gactccgcgg tcgtcgccat cgtccgccgc ggccacgtgg tcatccccca
     1681 gccagagacc acgctcgccg ccggtgacga ggtgatggcg atctcctcca ccgccagcga
     1741 gggccagctc cgccaggcga tcacgggcga gtagcgctca ggcgacggct gatcgttcag
     1801 ccacctcccg gcctggcgcg ggccctgtag cccgcaagca acaggatggt gccggcgacc
     1861 actcctccag caatgaagct caagcggaga gtcgtccgag catcgcgcga cgcatccatc
     1921 atgttgagct gactcaccgc tacgaatctc gctatgcaaa cgggggtgtg tctacctagc
     1981 acgtaaccgc tcgggcagaa gtatcgggac ggccaggcca gcgccagcac cacggaagcg
     2041 acagcgacca ccgcggcggc aacccacatg accacggagg cggggcccgc gacctttcgt
     2101 tccgcggagc gcctcgccag gcccttcacg aacctcacgc tagcgcggaa cttgcagctg
     2161 ttcggcgatc gacaacagat cgtccagcga gaactgaacg cccgttcgcc ctggaagcat
     2221 ctgcgtcagc atccgcacgg aatatctccc ggttacccac gtcaaggccc ctggaccgtg
     2281 cgcgtccgga tccgcgccca tcccgacgac tccacggaca gtcaccagac ggaatccgct
     2341 acccccgttc gctgcgtcat cggcaatcat ggccgcagcg tccgttccgt actcctccgg
     2401 cgtgcgggag tccggcacat agtcaagcgc aaggccggag tcgaacatca ggccgacgcc
     2461 ctccccagaa ctcgccgccc acaggactct ggactgcgac gcgatgccga caggcgccgg
     2521 cccgggacac ccgcaggcgg cgaggcgggc gagcgcctcg tcgaacatcg ggacgctcag
     2581 ggatcggcca cgcctcagtc tccgggacgg gagtcagtca tgcgaagtcg tagctcgggt
     2641 tgatcaggcg gcggacgccg cggtcctcgc cgaggacgcc ctcgaggacc aggcgcgagc
     2701 cgagcaccag gccggggatc gaggtccggc cgagccacac cgcggtgacc tcgccggtgc
     2761 cgtcggagag caccgcctcg agcgactgga acccctgcgt cggacggacg gtgatccgct
     2821 cgaccacgcc ggccagcttg acgtgctggc gcggggtggc ttctgtcatc cgcgcgctcc
     2881 cggggacggt cgaggcccac tgcagcgtct cctcggcgag gacctcctcg tcggtccggg
     2941 ccagtcgccg gaagatccgg ctcaggagtc ccacgccgtc gatccgtccg ctcccgcgcg
     3001 agcccgtcgc ccgatcgccg gctacgccac cgtggtggcc ttcgcgtccg ccgccttcag
     3061 ctccgcctcg agctccgagc gccgcgactt cgagacccag atcgcggcgg caaggatcag
     3121 ggccccgacg acgccgaccg cgatgcgcag cccggtgtcc ccggcgtggg agacgacgag
     3181 cggcgcggac agcagcgcca ccaggttcat caccttgatc agcgggttga gcgccggccc
     3241 ggccgtgtcc ttgaacgggt cgccgaccgt gtccccgatc accgcggcct tgtgctgctc
     3301 ggagcccttg ccaccgtagt ggccctcctc gatgtatttc ttggcgttgt cccaggcgcc
     3361 tccggagttc gcgaacatca ccgccagcag ctgaccggtg aggatcgcgc ccgccaggaa
     3421 cgctccgagc gcctcggcct tcaggaagaa cccggtgatc agcggcgcga gcacggcgag
     3481 caggccgggc gtcatcagct cgcgcagcga cgtccgcgtg catatgtcga ccaccttctt
     3541 gtagtccggc ttctcgctga agtccatgat cccggggtgg tcccggaact ggttgcgcac
     3601 ctcgaccacc acctgctgtg ccgcgcggcc gacggcgcgg atggcgagcg aggagaacag
     3661 gaacgccacc gccccgccca gcaacaggcc gaccagcacg tccgggtggt cgatgcgcag
     3721 gccctggaag ttcaacccgg cgcccttcag cgcttcctcg aacgagccga acagcgaggt
     3781 cgccgccagc accgcggtgg cgatggccat ccccttggtg atcgccttcg tcgagttccc
     3841 gatggcgtcc aggccgccaa ggatcaccgc cggccggccc ttgaactcgc ccgacatctc
     3901 ggcgatcccc tgcgcgttgt ccgacaccgg gccgtaggtg tccatcgaca ccaccacacc
     3961 gaccgtggtc agcatcccca tgccggccag cgagatgaag tagagacgct cggcgttggt
     4021 gtccccgagc aggaacgccc cgatcaccgc cccggcgatg atcaggatcg accagaccgt
     4081 ggactccaga ccggtggaga atccggacag gatcgtcgtc gccggcccgg tcaccgtggc
     4141 ctcggcgatc tcccgaaccg gcttgtactt cgtgtcggtg aagtactggg tcaggacctg
     4201 gatgatgctc gccagcacca ggccgagcag cacggcgacg gccggcgccc acgagtcgag
     4261 gtagaccttc gagatggcga acacggccgc cgccgacagc gcggcggaga ggaagaagcc
     4321 gcggttgatc gcgcgcatcc cgtgctcctc ctcgctgcgc ggcgccaccg ccaggatgcc
     4381 gacgatcgag gtgacgacgc cgaccgcgcg gacgaacagc gggaacatca cgccgacgat
     4441 ggcgcccttc ggcgagccgg cgaacgccgc cgccccgagg atcagcgacg ccaccagcgt
     4501 gacctcgtag gactcgaaca ggtcggccgc catcccggca cagtcgccga cgttatctcc
     4561 gacgttgtcg gcgatgacgg ctgcgttgcg cgggtcgtcc tccgggatgc cctgctcgac
     4621 cttgccgacc aggtcggcgc cgacgtcggc cgccttggtg aagataccgc cgccgacccg
     4681 catgaacatc gccagcagcg ccccgccgaa accgaacccg accagcaccg aggtggcgtc
     4741 cgacttgtag atcagcagga tcgtggtcgc gccgagcagc ccgaggccga ccgtgaacat
     4801 gcctgcaacc ccgcccgcgc ggaaggcgat cttcatcgcc cggcgcaggc cgctc
//
LOCUS       DASPWO010000228         6438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_455483, whole genome shotgun sequence.
ACCESSION   DASPWO010000228 DASPWO010000000
VERSION     DASPWO010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455483"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VEO00_05085"
     CDS             complement(<1..253)
                     /locus_tag="VEO00_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014673943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HYU57407.1"
                     /translation="MRSPGRVLTVLAVVAAAIGVPASGRAAPTGRPAAAHAVSAAPSD
                     PPADFNGDGYGDLAVGVPGENIGSVANAGAVNVIYGSPTG"
     gene            complement(474..547)
                     /locus_tag="VEO00_05090"
     tRNA            complement(474..547)
                     /locus_tag="VEO00_05090"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(513..515),aa:Gln,seq:ctg)
     gene            complement(716..1150)
                     /locus_tag="VEO00_05095"
     CDS             complement(716..1150)
                     /locus_tag="VEO00_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYU57408.1"
                     /translation="MREIRTEIEIGASVQRVWSVLTDLEDFGEWNPFIVRADGELLPG
                     ERLVLTMRLPGRRARTFRPTVLAHDPNRELRWLGRLGIPGIFDGEHAHRVQALPDGRT
                     RYVQEEGFRGILVPFVGGILRATEEAFRGMNEALKTRAEAQS"
     gene            1423..2019
                     /locus_tag="VEO00_05100"
     CDS             1423..2019
                     /locus_tag="VEO00_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57409.1"
                     /translation="MTALVLAVLVATVLLPVGAQAAQLLNVAITDPGGVNQATVDSGG
                     NLHVSGEVTLDSSAPVAVTSADDPEREAFQAEVLITITPGETGGGANIDVPAGKRLLI
                     THLSGEVKLPAGQELIRVVLSTTGGEGDVVVIHHFVPTPTGTIGVEDVFAVSQETMIH
                     DDGPQFLVSVTRNTNVDTGTAIVAVSGYLVDCSIAPCN"
     gene            complement(2078..3634)
                     /locus_tag="VEO00_05105"
     CDS             complement(2078..3634)
                     /locus_tag="VEO00_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYU57410.1"
                     /translation="MLGSIRSGNFGVGSWIERRARFAPDDVALIAVDRSSTYAELRTR
                     IRRLANGLRQLGVARGDRVAWLGPNHPAFLEALFATGLLGAVLAPVNHRLQAAEILPV
                     LEDTEPTVLIQHRADPLPVPPSVRHRVDVTGSLDTALAFEQLVAESPDDAIEEPVGLE
                     DLCLLPHTSGTTGRPRQVMLTHGNVTWNVVNFLTCADFRSNDVTVAIAPFFRVGGTGV
                     NVLPVLFIGGTVVVPTDPDAEEILRLIERHRVTVGFGNPDILDALVRSQLWMDVDLSS
                     IRFVLTGGAPVPERLIRAYLERGVTLLQGYGLSEAAPLALLLDPESALQKIGSAGRPP
                     LLVDVRIESAAGVAVEPGETGELLVRGPNVMAGYRNRPEATREVLTSDGWLRTGDAAR
                     VDDEGFVWIVDRVRDGFTTDGYRVYPGDIERALGGHPAVADVGVVGVLAQARGEVGAA
                     FVVLTNGAEATERELLAFCDQRLAPHQVPASVTFVDRLPRSSVGKLIRDQLRALASRG
                     PADGIGRPAV"
     gene            4408..4977
                     /locus_tag="VEO00_05110"
     CDS             4408..4977
                     /locus_tag="VEO00_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylase"
                     /protein_id="HYU57411.1"
                     /translation="MVSVGWPRAEFDEHRRHLEASFWDVLARFGIEERVRAGRRQEPL
                     RGTGDVPNRYRRSHGPGWALIGDAGYYKDPVTALGIADAFRDAEAVAGAIHSALAGKR
                     PVDEALADYEARRDAATRAHFEFTTRVARLPALDPASRAMLSGFRDHADLAEGYFRVF
                     AGLLEWPRLFTPENVTRAVSGPSAIRPQA"
     gene            5123..5464
                     /locus_tag="VEO00_05115"
     CDS             5123..5464
                     /locus_tag="VEO00_05115"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HYU57412.1"
                     /translation="MNKDALVAEVAKRSGAAKAEVAKIVDAMTDTIRGTVARGQKVAL
                     VGFGTFERRARNPRTARNPRTNEELRVPRTNVPQFRPGAAFKEAVAGKRRAKAPVRKR
                     AVARKAAGRTR"
     gene            complement(5551..>6438)
                     /locus_tag="VEO00_05120"
     CDS             complement(5551..>6438)
                     /locus_tag="VEO00_05120"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYU57413.1"
                     /translation="QEDWVADGWWTGLARQAAAILPGVTPELVREHLIPPMFDPDGSA
                     QLGAMEQAGIDVAIVFAFDWSTGPHWGPPAPVGWREQNAWYRDLAAANPGRIRWAFGA
                     DPRHPGAALALRRAVREDGAVAMKIHPGNGFDLDDPAVYPAVEAAGELDVPVICHVGP
                     ISAPGRSRWAAPERLDTLAASFPEVRFVAAHCGNALWREVLAFCSVSPNVWVDLSGWQ
                     IRFQRNPDRFHADVREVLEELGPGRVMWGSDAPHYRPAVPDDEYLRAFTDAPPGTYTA
                     EEVEAITGATAATLFRLPS"
BASE COUNT          967 a         2276 c         2286 g          909 t
ORIGIN      
        1 ggccggtggg ggagccgtag atcacgttca ccgcccccgc gttggcgacc gagccgatgt
       61 tctcgcccgg aacccccacc gccaggtcgc cgtagccgtc cccgttgaag tccgccgggg
      121 gatcggacgg agccgcggag acggcgtggg cggcggccgg tctgccggtg ggggcagccc
      181 ggccggaggc agggaccccg atcgccgccg caacgaccgc gaggaccgtg aggacccggc
      241 cgggagagcg catccctgcc tcctaagact tatcgaggcg ggctcccgtg gcgggcctga
      301 tcggatgctg acacgatggg gcgaacgtgt ccacggacca cgcgcagggc aacgctcatg
      361 gcctcgtcgg tagaggatga cgacgagcat cgccgggatc atcaagccgc agttcagtct
      421 gcacgccgga tgatcgcgcc ggtctcatca gatcggttca gcgacaagca ccttggctgg
      481 gacgccagga ttcgaacctg gactaactgg tccagagcca gtcgggctac cgttacccca
      541 cgtcccaccg tatgacctgg acctcccaat caacccgagt ggcagcgctc tggtgagcga
      601 ctactcggag actgcgatga ccagggtcac ggttgcgagg ttagcagagg gggcgacacg
      661 gatacgctcc gcggagctcg aggtcccttc acggttccgt catccggcat ctctttcagc
      721 tctgcgcctc cgcccgggtc ttgagggcct cgttcatccc gcggaacgcc tcctcggtcg
      781 cgcggaggat cccgcctacg aacgggacca gaatgccgcg gaagccctcc tcctggacgt
      841 acctggttcg cccgtcggga agggcctgga cccggtgggc gtgctcgccg tcgaagatgc
      901 ccggaatccc cagacggccg agccaacgga gctcccgatt gggatcatgg gcgagcaccg
      961 tcggacggaa cgtcctggcc cttcgtccag ggaggcgcat cgtcaggacc agccgttccc
     1021 cgggcagcag ctcgccatcc gcacgcacga tgaacgggtt ccattccccg aagtcctcga
     1081 ggtccgtgag gacactccag acgcgctgga ccgacgcgcc gatctcgatc tccgtcctga
     1141 tctctcgcat cgctcctccc gtccggcgtg ctccaacctg gtcagcctat ccacgcccgc
     1201 ggatcgagag gagtctgtcc cgcttgttcg gttcaagagc cgctgctagc gtcgctgcga
     1261 acgaatcatg gccgctgggc ctctcgcacg ctccccaccg cccgccgctt cctgacgtgt
     1321 ggccgtctgc ccgccatgat cggagggaag gcgatgaggc gtcgcggaca gtccagcggt
     1381 acgaatgacc ttcttcccgg atccacgttc acggtacggc aggtgaccgc cctggtcctg
     1441 gccgtgctgg tggcaacggt cctgctcccg gtcggggcgc aggcagcaca gttgctcaac
     1501 gtcgccatca ccgacccggg gggcgtcaac caggccacgg tcgattccgg gggcaacctc
     1561 cacgtgagcg gggaggtgac cctggactcc tccgctccgg tggccgtcac gagcgcggac
     1621 gacccggaga gggaggcgtt ccaggcggag gtgctgatta ccatcacgcc cggggagacg
     1681 ggcggcggag ccaacatcga cgtccccgcc gggaagcggc tgctgatcac ccatctctct
     1741 ggggaggtca agctgccggc cggacaggag ctcatccggg tcgttctttc gacgaccggc
     1801 ggtgaggggg acgtcgtcgt catccaccac ttcgttccga cccctaccgg aacgatcggc
     1861 gtcgaagatg tcttcgcggt gtcccaggaa accatgatcc acgacgacgg cccccagttc
     1921 ctggtctcgg tgacgcgcaa caccaacgtc gacacgggga cagccatcgt tgccgtctcc
     1981 ggctacctcg tcgactgctc gatcgcgccc tgcaactgag tcgagatggc cggccggagg
     2041 gaggggccga ggtctcttcg gttggagccc ggtccgccta cacggccggc cgtccgatgc
     2101 catcggcagg gccccgggag gcgagggctc gaagctggtc cctgatcagc ttccccacgg
     2161 aactccgcgg caggcgatcc acgaacgtca ccgaggcagg gacctggtgg ggggcgaggc
     2221 gctggtcgca gaacgccagg agctctcgct ccgtcgcctc cgctccgttc gtcaacacga
     2281 cgaaggccgc tcccacctcg cctctcgcct gtgccaggac gcccaccacc cccacgtccg
     2341 cgaccgccgg atgcccgcca agcgctcgtt cgatgtcgcc cgggtacaca cgatacccat
     2401 cggtggtgaa gccatcccgc acgcgatcga cgatccagac gaacccctcg tcgtccacgc
     2461 gcgccgcgtc tccggttcgg agccacccat ccgatgtcag gacctcgcgc gtggcctccg
     2521 gccggttccg gtacccggcc atgacgttcg ggccacgcac cagcagctcc ccggtctccc
     2581 cgggctccac ggccacaccg gccgcgctct cgatccggac atcgaccagc agcggcggcc
     2641 tgccggcaga cccgatcttc tgaagggcgc tctccggatc cagcaggagc gcaagcggcg
     2701 ccgcttcgga cagtccgtac ccctgcagca aggtcacgcc tcgctcgaga tacgcgcgga
     2761 tgagccgttc gggaaccggg gcaccgccgg tgaggacgaa tcggatgctc gagaggtcga
     2821 cgtccatcca gagctgggat cgcaccagcg cgtccaggat gtcgggatta ccgaagccca
     2881 cggtgacccg atgacgctcg atcagtcgga ggatctcctc agcgtccggg tcggtcggca
     2941 ccaccacagt tccgccaatg aacaggaccg gcaggacgtt cacccccgtc ccaccgaccc
     3001 ggaagaaggg ggcgatggcc accgtgacgt cgttgctgcg aaagtcggcg caggtgagga
     3061 agttgacgac gttccaggtc acgttgccgt gcgtcagcat cacctgtctc ggccgccccg
     3121 tggtcccgga ggtgtgcggg agcagacaca ggtcttccag gccgaccggt tcctcgatcg
     3181 cgtcgtctgg cgactcggcc acgagctgct cgaaggccag cgcggtgtcc agggagccgg
     3241 tcacgtcgac tcggtgccgg accgaggggg ggaccggcag ggggtccgca cgatgctgga
     3301 tcaggacggt cggctcggtg tcctcgagaa ccgggaggat ctctgccgcc tgcaaccgat
     3361 ggttcaccgg ggccagcacc gccccgagca ggcccgtggc gaagagtgcc tccaggaacg
     3421 cgggatgatt cggccccagc cacgccactc gatcgcctct ggccacgccc agctgccgga
     3481 gcccgttggc cagtcgccgg atccgggtgc gcagttccgc atacgtggac gagcgatcga
     3541 cggcgatcag cgccacgtcg tcgggggcga accgcgcccg acgctcgatc cacgagccga
     3601 ccccgaagtt tcccgatcgg atgctcccga gcatgccccc aggctacagg ggagggcgtc
     3661 gatgcaggcg cgcacgggcg acccggcgtt tcgaggtgga tcgcccagag tcggtcgggc
     3721 taccgttccc tccggtcccg ggaacggata gaggatatcg gccgcatgta cgacgccatc
     3781 gtggtcggtg ctcggtgcgc ggggctcgcc ggccgcgatg ctgctggcgc ggctcgggca
     3841 tcgcgtgctg ctcctcgacc gggcccgctt cccgagcgac acgatctcat cgcactacct
     3901 ccagccccag gccgtggcgg cgctatggcg ctggggcctg ctcgacgacc tgctggcgac
     3961 gggctgtccg ccgatccagc aggggcggtt cgcgttcgac ggcttcgcgc tcgaggcgcc
     4021 ggttccgacg cacgacggga ccgagttcac gctgagcagc cgccgcacct acctcgacga
     4081 gctgctcgtt cacgcggccg gggacgccgg cgtcgaggtc cgggagcggt tcgcggtccg
     4141 cgacctcgtg tcggaccacg gccgggtggc cggcgtgcgc ggggaggcgg acgggcggga
     4201 aacggaggag cgcgcggcgg tcgtcgtcgg ggccgacggc gtccactcgc gcatcgcgcg
     4261 gctggtcgag tcgccgatcc acgaggatcg gggccgctgg cgtgctggta ctactcctac
     4321 tggaccggcg tcgatccgct gggcttcgag ctcttcgccg cgccggcgcg gggggcggcg
     4381 atcaatccga cccaggacgg cctggcgatg gtctccgtgg gctggccgcg cgcggagttc
     4441 gacgagcacc gccgccacct cgaggcctcg ttctgggacg tgctggcccg cttcgggatc
     4501 gaggagcgcg ttcgcgccgg gaggcgccag gagcccctgc gcgggaccgg cgacgtcccg
     4561 aaccgctacc ggcgctcaca cgggcccggc tgggccctga tcggcgacgc cggctactac
     4621 aaggatccgg tcacggcgct cgggatcgcc gacgcgttcc gcgacgcgga ggccgtggcc
     4681 ggggcgatcc actcggcgct cgccgggaag cggcccgtcg atgaggcgct cgccgattac
     4741 gaggccaggc gtgacgcagc gacccgggcg cacttcgagt tcaccacgcg ggtggcgcgg
     4801 ctgccggcgc tcgatcccgc gtcccgggcg atgctgtcgg gcttccggga ccatgccgac
     4861 ctcgccgaag gctacttccg cgtcttcgcc ggcctgctcg agtggccacg gctgttcacg
     4921 ccggagaacg tgacccgggc ggtctccgga ccgagcgcca tccggccgca ggcgtgacgt
     4981 tccgtcatgg atggggggtc tgctcttcgg acgtggtcgg tcagatgccg acccagggcg
     5041 agctcccccc aatcctctgg cgttgacggc gttcgccgcc cgtggctaag gtgccgcgtc
     5101 gccccggcgg cggagggagc gggtgaacaa ggacgcgctg gtcgctgagg tcgccaagcg
     5161 aagcggcgcc gcgaaggccg aggtggcgaa gatcgtggac gccatgaccg acacgatcag
     5221 gggaaccgtg gcccgcggtc agaaggtggc cctggtcggg ttcggcacgt tcgagcggcg
     5281 cgcacgcaac ccgcgcacgg cgcgcaaccc gcgcacgaac gaggagctca gggtgccgag
     5341 gacgaacgtc ccgcagttcc ggccaggcgc ggcgttcaag gaggcggtcg ccggcaagcg
     5401 ccgggcgaag gcgcccgtca ggaagcgggc cgtggctcgg aaggcggccg gcaggacgcg
     5461 gtaggcgggg gaaacagatg gtcaaggtcc ttgaccatct cggcgccgcg acgggaagga
     5521 gccgggccac gcgcccggct cctcccgctt tcaggacggg agccggaaga gggtcgcggc
     5581 cgtggcgccg gtgatcgcct cgacctcctc ggcggtgtag gtcccggggg gggcgtcggt
     5641 gaaggcccgc aggtactcgt cgtcgggcac cgccgggcgg tagtgaggcg cgtcggagcc
     5701 ccacatcacg cggccgggcc cgagctcctc gagcacctcg cggacgtcgg cgtggaagcg
     5761 gtccgggttg cgctggaagc ggatctgcca gccggagagg tcgacccaca cgttcggcga
     5821 caccgagcag aacgccagca cctcgcgcca gagggcgttg ccgcagtgcg cggcgacgaa
     5881 gcgcacctcg gggaacgagg ccgcgagggt gtcgaggcgc tccggcgccg cccagcgcga
     5941 gcggccgggg gcgctgatcg gcccgacgtg gcagatcacc ggcacgtcga gctcgcccgc
     6001 cgcctccacg gccgggtaga cggcggggtc gtccaggtcg aacccgttgc ccgggtggat
     6061 cttcatcgcc accgcgccgt cctcccgcac cgcccggcgc agggccaggg cggcgccggg
     6121 gtgacggggg tccgcgccga acgcccagcg gatccgcccc gggttcgccg ccgcgaggtc
     6181 gcggtaccac gcgttctgct cccgccagcc gaccggcgcc ggcggacccc agtgcgggcc
     6241 cgtggaccag tcgaaggcga agacgatcgc cacgtcgatc ccggcctgct ccatggcgcc
     6301 gagctgcgcc gagccatcgg ggtcgaacat cggagggatc aggtgctcgc gcaccagctc
     6361 gggagtgacg ccggggagga tcgctgccgc ctgcctggcg agcccggtcc accagccgtc
     6421 ggcgacccag tcctcctg
//
LOCUS       DASPWO010000229         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_965172, whole genome shotgun sequence.
ACCESSION   DASPWO010000229 DASPWO010000000
VERSION     DASPWO010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965172"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VEO00_05125"
     CDS             <1..558
                     /locus_tag="VEO00_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010274792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HYU57414.1"
                     /translation="EILEAGALPLRYTGFSTNFRREAGAAGRDTRGMFRVHQFDKVEM
                     FSFTLPEASWDEHEFLLACQEEIVGGLGLPYRVVNVCVGELGASAAKKYDIEVWLPGQ
                     GTYRELTSCSSCTDYQARRLQARYRAEGENRPMHTLNGTACAVGRTIIAVLENGQRED
                     GSVALPEALHPYLPPGMEILTPVQP"
     gene            complement(549..1322)
                     /locus_tag="VEO00_05130"
     CDS             complement(549..1322)
                     /locus_tag="VEO00_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU57415.1"
                     /translation="MSLRTSGAIIRHEFRVMMTDPSTVIFLIFIPLFMVAFMRGLFAG
                     ALADAGFPGANGSEFAIPGMAVAFAAFGVSWAGFTFFRDHGWGTWDRLRATPITSVDL
                     IAGKVIPNVAVTVVQLGALFVIGGPLFGLRVQGSIFGLALTVLVLALALSAFGMAVTS
                     VSRTMQQLNAIGSVGAFAMAMIGGAWVPVESMPGWGQAIAPVMPTYWGMKAFRSVILE
                     GGGIAQIALPLLVIVGFGVLFTLLAAAKFRLDDTKAYYG"
     gene            complement(1306..>2071)
                     /locus_tag="VEO00_05135"
     CDS             complement(1306..>2071)
                     /locus_tag="VEO00_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU57416.1"
                     /translation="IVEVDGLDALKRTAEVRRRIGLAPQDLGIYPVVSVRNNLLLFGR
                     LAGLGKAELKKRIDEVAEALDIADLLDRQGGTLSGGQKRRAHTAIALLHRPPLLLLDE
                     ATTGADVETRQHILNLVRGLAADGSAVLYSTHYLKEVEDLDATVVLLDEGRVIARGRA
                     EELVAAHATPVVDLTFEGDVPPVKLDGQASIDGSRLRVQTADPAATLASVLPRLPANS
                     LAAVEIVRPDLEAVYLALTGRRYDEADKEAEHVAAN"
BASE COUNT          336 a          729 c          729 g          277 t
ORIGIN      
        1 gagatcctgg aggcgggcgc gctaccgctg cggtacacgg ggttctcgac gaacttcagg
       61 cgcgaggcgg gcgccgcggg gcgcgacacc cgcgggatgt tccgcgtcca ccagttcgac
      121 aaggtcgaga tgttcagctt cacgctgccc gaggcctcgt gggacgagca cgagttcctg
      181 ctggcctgcc aggaggagat cgtcggaggg ctggggctgc cgtaccgcgt ggtgaacgtc
      241 tgcgtcggcg agctgggggc ctcggcggcg aagaagtacg acatcgaggt gtggctcccg
      301 ggccagggga cctatcgcga gctgacgtcg tgctcgagct gcaccgacta ccaggcccgc
      361 cgcctgcagg cgcgctaccg cgccgagggc gagaaccgcc cgatgcacac gctgaacggc
      421 accgcctgcg cggtcggtcg gaccatcatc gccgtcctcg agaacggcca gcgcgaggac
      481 ggctcggtgg cccttcccga ggccctccac ccgtacctgc cgccagggat ggagatcctg
      541 acgccggttc agccgtagta ggccttggtg tcgtcgaggc ggaacttcgc tgctgccagc
      601 aacgtgaaca gcaccccgaa gccgacgatg acgaggagcg gcagggcgat ctgggcgatg
      661 ccgccgccct cgaggatcac cgaccggaag gccttcattc cccagtaggt cggcatgacc
      721 ggggcgatcg cctgacccca gcccggcatg gactccaccg gcacccaggc gccgccgatc
      781 atcgccatcg cgaacgcgcc gacgctgccg atcgcgttca gctgctgcat ggtccgtgac
      841 acggaggtca ccgccatgcc gaacgcggag agcgcgagcg cgagcaccag gacggtcagg
      901 gcgagcccga agatcgaccc ctgcacgcgc aggccgaaca gcggcccacc gatcacgaac
      961 agggccccga gctggaccac cgtgaccgcc acgttcggga tgaccttccc ggcgatcagg
     1021 tccaccgacg tgatcggggt cgcccgcagc cggtcccacg tcccccagcc gtggtcgcgg
     1081 aagaacgtga acccggccca ggagacgccg aacgcggcga acgcgacggc catccccggg
     1141 atggcgaact cggacccgtt cgcgccggga aagcccgcgt ccgcgagggc gccggcgaag
     1201 agcccgcgca tgaacgccac catgaacagc gggatgaaga tcaggaagat gacggtcgag
     1261 gggtcggtca tcatcacccg gaactcgtgc cggatgatcg ccccgctagt tcgcagcgac
     1321 atgctccgcc tccttgtcgg cctcgtcgta gcgccgtccg gtgagcgcca ggtagacggc
     1381 ctccaggtcg ggacggacga tctcgacggc cgccaggctg ttcgcgggaa ggcgggggag
     1441 gacggacgcc agcgtggcgg ccggatcggc ggtttggacg cgcagccgcg acccgtcgat
     1501 gctcgcctgg ccgtcgagct tcaccggcgg cacgtcgccc tcgaacgtga ggtccacgac
     1561 aggggtggcg tgcgcagcca cgagctcctc ggcccgcccc cgcgcgatca cgcggccctc
     1621 gtcgagcagc accaccgtgg cgtcgaggtc ctcgacctcc ttcaggtagt gcgtcgagta
     1681 gagaaccgcc gacccatcgg cggccagtcc ccggacgagg ttcaggatgt gctggcgggt
     1741 ctcgacatcg gcgccggtgg tggcctcgtc cagcagcagc aggggcggtc ggtgcagcag
     1801 cgcgatggcg gtgtgggcgc gccgcttctg tccgcccgac agcgtgcccc cttgccggtc
     1861 gagcaggtcc gcgatgtcga gcgcctcggc aacctcgtcg atccgcttct tcagctccgc
     1921 cttcccgagc ccggccaggc gtccgaacaa cagcaggttg ttgcggacgg agaccaccgg
     1981 atagatgccc aggtcctgag gcgccaggcc gatccgccgg cggacctcgg cggtgcgctt
     2041 cagggcgtcg agcccgtcca cctccacgat g
//
LOCUS       DASPWO010000230         8727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1219281, whole genome shotgun sequence.
ACCESSION   DASPWO010000230 DASPWO010000000
VERSION     DASPWO010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219281"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VEO00_05140"
     CDS             <1..348
                     /locus_tag="VEO00_05140"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYU57417.1"
                     /translation="KFMLLIYGEEAAAARATDGERQAEAAAYEEFTQSIRASGNYLDG
                     DPFLPTSAARTVQLRGGEAKSTDGPAVNSAPQLLAYYKVEAETAEQAVEMASRIPGAR
                     FGSIEVRPVMQFD"
     gene            complement(353..1135)
                     /locus_tag="VEO00_05145"
     CDS             complement(353..1135)
                     /locus_tag="VEO00_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU57418.1"
                     /translation="MGDVTHYPNGTFCWIDLATPDVAGARAFYGDLLGWEMDDLPAEQ
                     GGTWTMCRLRGKAVAAIHEDPDEPAEWDSFISVDDVDATTLRAGELGATVLVDPFDVP
                     DAGRMSVLRDPVDAVVSLWQPRGRIGAEYVNDVGAWNWNELVTPEIDRATAFYGELFG
                     WTSQELPVQIRRASLQLGDLLIGGVHAPTPPEGDAARWTISFTVDDADRAAARTEALG
                     GRVLLPPMAIPNGTFTIVADPAGAAITLAAAPGGPLRGVDGS"
     gene            complement(1210..1497)
                     /locus_tag="VEO00_05150"
     CDS             complement(1210..1497)
                     /locus_tag="VEO00_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57419.1"
                     /translation="MGEHHEVMHDRLYLKEPGTERVRKRAAARYAHLLSTGWRETERW
                     HAPNYITVRMERSGVVPRKELRAVPPPPRTQRRDARGGFPGRGGFPGRGGR"
     gene            complement(1590..2762)
                     /locus_tag="VEO00_05155"
     CDS             complement(1590..2762)
                     /locus_tag="VEO00_05155"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HYU57420.1"
                     /translation="MTRAVVLSAVRTPVGRYGGALSGVRPDDLAAVAIAAAVERAGVE
                     PAEIEDVYLGCANQAGEDNRNVARMAGLLAGLPESVAGVTVNRLCASGLSAVASACHA
                     ILAGDGDLFVAGGVESMSRAPLVMAKPERAFPRGNRTIFDTTLGWRFPNPRMEALFPL
                     ETMGETGENVAERWTVSREEQDAFALRSQRRWAAAQAAGRFDDEIVPVEGVDTDEHPR
                     PDTTTEKLAALAPAFREGGSVTAGNASGINDGAAALVIAGEERARAIGTPPLGAFAGS
                     AVAGVDPRVMGIGPVPAVRKLLARAGVDPSEIDLVELNEAFASQSLVVIRELGLDPER
                     VNVNGGAIAIGHPLGMSGARLAVTLLHELRRRGGRYGLATLCVGVGQGQAVLFERG"
     gene            complement(2759..3784)
                     /locus_tag="VEO00_05160"
     CDS             complement(2759..3784)
                     /locus_tag="VEO00_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57421.1"
                     /translation="MSDDTVPRATLIPSWSERYLGGSEAREADLLRAYAAQIQRVQRR
                     NRRGDEQIRRGFHAKLLAGIATARFVVAENVRPELRVGLILPGASYPAMVRYSSASGV
                     AASRSIKDLRGIAIRVQTGGERGDRVQDFLLSDTPTSHARDARQFMAAADAMAGGRRL
                     TALPGLIGKLGPWEGVRVLRALRHASAQLIDSVATVPFFSRAPFAIGPYAVKFKLQPV
                     APPAERVGRLGHDELRRDLIERLRREAVRYELQVQHYVDDRTTPIEDGTVEWPEPGRP
                     ETLAELVIPRQDLASAEGQEGERLVEALEFSPWRTDGTIRPIGGLNRARKPVYEASAR
                     LRSGGSA"
     gene            3819..5213
                     /locus_tag="VEO00_05165"
     CDS             3819..5213
                     /locus_tag="VEO00_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HYU57422.1"
                     /translation="MVAATKRRAIASADMAGPPLPPGPRTWGVLSNAISAARFAPGYL
                     QRVAARYGDIACIKAGPVRVYLLSSPELAQDLLETNEARFEKGRAEQRVTRRLLGEGV
                     LASEGEFHRRQHALIEPLLHGAALERFAPIVVERAVRMQEAWQDGQVVDLWDRLIRTM
                     TEIMVEALFGVSVDAPAGAELLQLLNGAVDAVERLPFAALRIGERLPFPRNRRFEEVR
                     ARLDAVLLGLVAERRGDRRQRGDLLSSLARARHPDGEPMPDRLVRDEALTLFRAAKTT
                     GTALSWTWHLLSRHPDPEARLLDEIDTVVGDELPSASDLARLTYCRRVLDEALRLFPP
                     AWISGRRPVAEHEIGGYVIPVGSTVATSPWVIHRDARVHPAPERFDPDRFSPERRAGW
                     HPFAYFPFGGGSRTCLGDEFAPLEALMLLATVGRRWRLRPVPGHEVRPAAKAILRPRG
                     GLPVVLQRRAQPVR"
     gene            5397..5639
                     /locus_tag="VEO00_05170"
                     /pseudo
     CDS             5397..5639
                     /locus_tag="VEO00_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(5722..6426)
                     /locus_tag="VEO00_05175"
     CDS             complement(5722..6426)
                     /locus_tag="VEO00_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYU57423.1"
                     /translation="MRSTEVVAGVRRMWFPSVNNNAYLIAGREATLIDCGPPRDAVEV
                     LEPLRQLGLRPSDVRHVAVTHCHTDHTGQLAAVVRATGATVYAHARDARIVRDGAERP
                     RGNAHGPTGWIMRLLARGPSRADAAPVAALVEDGQELPAAGGLRCIHTPGHTAGHVSF
                     LWPEGAVLFVGDAAANMLRTLDIAPLNDDDVAARASFRRLAELDFDVACFGHGPPIRS
                     GAAPRFRRKLERVANR"
     gene            complement(6429..7292)
                     /locus_tag="VEO00_05180"
     CDS             complement(6429..7292)
                     /locus_tag="VEO00_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57424.1"
                     /translation="MSPEAVETGAVKPFSDAMRASVVGPATRGLLGALLFLLSTRTAD
                     YFAWTIATPLTAATLGANYWASTVLAILASRERYWANGRISISVAFVFSPLMTAATFL
                     HLGEFHFDAGGVAVVITWFWVLAYGFYPIQLTVQMVQQLRLPGGDPPRTDPLPLWVKT
                     VLGAHALVFIPLGVLTFVAPGVGGPLWPWEIGAPLALRALSAWVLAFGVGALHAILEN
                     DFDRVKVALWGYPVLGALQLIALVRFGEVLQTNEAGWWFYLAFLASAFVLGAYGYVMA
                     RRLRSRRAATA"
     gene            7311..8054
                     /locus_tag="VEO00_05185"
     CDS             7311..8054
                     /locus_tag="VEO00_05185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYU57425.1"
                     /translation="MSSVTEIPLPPEDLWRVGRGGDFRLIGRDLVDLLVYHGLRPDHR
                     VLDVGSGIGRVAAPMTAYLTTGSYEGFDVDREMVAWCRRNITMRFRNFRFQVADLYNK
                     QYNPTGRQRAETYRFPYEDASFDYVLLTSVFTHMPPKEVDHYLSEISRVLVPGGIASI
                     TWFLVDQDSRKILIGRDRGPDFRFRRRKHRIANRRNPAAAVAHDLRNVRAMYRRNGLW
                     IRELRRGSWRGSAGGPGATHQDLIVATRR"
     gene            8112..>8727
                     /locus_tag="VEO00_05190"
     CDS             8112..>8727
                     /locus_tag="VEO00_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU57426.1"
                     /translation="MEFGVCFSADPPTRRWVDLSALAERHGFDYVWMWDSHVLWREVY
                     PLFTLIGEATERVRIGPLVTNPVTRDPTVTASVLATLDELNGGRMVMGIGRGDSARRV
                     IGQTPVGVERMEWASGLIRDLVAGRQVDYEGAPVKLEWAHWDLPVWMAAYGPKALRAV
                     GRVADGLIMQLADPFIIEWSLAYVRQGAEEAGRDPGAVKIMSAAP"
BASE COUNT         1197 a         3186 c         3183 g         1161 t
ORIGIN      
        1 aagttcatgc tgctgatcta cggcgaggag gcagcggcgg cgcgggccac cgacggagag
       61 cggcaggccg aggcggctgc gtacgaggag ttcacccagt cgatccgggc gagcgggaac
      121 tacctggacg gcgatccgtt cctgcccacg agcgcggcga ggaccgtcca gctccggggc
      181 ggcgaggcga agtccaccga cggcccggcc gtgaacagcg cgccgcagct cctggcgtac
      241 tacaaggtcg aggccgagac ggctgagcag gccgtcgaga tggcgtcgcg gatcccgggg
      301 gcgcgcttcg gctcgatcga ggtccgcccg gtgatgcagt tcgactgagg cgtcaggagc
      361 cgtcgacgcc gcgcagcggc ccgccggggg cggcggcgag ggtgatcgcc gccccggcgg
      421 gatccgcgac gatcgtgaac gtgccgttcg ggatggccat cggcgggagc aggaccctac
      481 ccccgagcgc ctcggtgcgc gccgcggccc ggtcggcgtc gtccacggtg aacgagatgg
      541 tccaccgcgc cgcgtcgccc tcgggcggcg tgggagcgtg gacgccgccg atcagcaggt
      601 cgccgagctg gaggctcgcc cggcgaatct ggacggggag ctcctggctg gtccatccga
      661 acagctcccc gtagaaggcg gtcgcccggt cgatctcggg agtcacgagc tcgttccagt
      721 tccaggcgcc gacgtcgttc acgtactcgg cgccgatgcg gccgcgcggc tgccagaggg
      781 agacgactgc atcgacgggg tcccggagca cggacatccg gccggcgtcg ggaacgtcga
      841 acgggtccac cagcacggtc gccccgagct cgccggcgcg cagggtcgtc gcgtccacgt
      901 cgtcgaccga gatgaaggag tcccactccg cgggctcgtc cgggtcctcg tggatcgccg
      961 ccacggcctt cccgcgcagc cggcacatcg tccacgttcc gccctgttcg gccgggaggt
     1021 cgtccatctc ccagccgagc agatcgccgt agaaggctcg cgccccggcg acgtcgggcg
     1081 tggcgaggtc gatccagcag aacgtcccgt tcgggtagtg ggtgacgtcg cccatcgccg
     1141 aaacgctacc gcgccggagc ggcggcggct acgtcgtgga tgcggacttc cgcgagcggt
     1201 tggtgcaggc tatcggccgc cgcggcccgg gaagccgccg cgaccgggga acccgccacg
     1261 ggcgtcacgg cgctgggtgc gaggcggcgg cggcacggcc ctgagctcct tgcgcgggac
     1321 gacgcccgac cgctccatgc gcaccgtgat gtagttcggc gcgtgccagc gctcggtctc
     1381 gcgccatccg gtggaaagga ggtgggcgta gcgtgccgcg gcgcgcttgc gcacccgctc
     1441 ggtgcccggc tccttcaggt acaggcggtc gtgcatgacc tcgtggtgct cgcccatggg
     1501 gggggctcct ctcgtcgcga cgggggtcgg tggtcccgat caagtgtagg gactcgcgct
     1561 cgccacgcgc ggccggactc cgagcggcct cagccgcgct cgaacaggac cgcctggcct
     1621 tgcccgaccc cgacgcagag cgtggccagg ccgtagcggc cgccccggcg ccggagctcg
     1681 tgcagcaggg tgaccgccag ccgcgcgccg ctcatcccga gcgggtggcc gatggcgatg
     1741 gcgccgccgt tcacgttgac gcgctccggg tcgaggccca gctctcggat caccacgagg
     1801 ctctgtgacg cgaaggcctc gttcagctcg accaggtcga tctccgacgg gtccaccccg
     1861 gcgcgcgcca ggagcttccg caccgccggc accggaccga tccccatcac tctcgggtcg
     1921 acgccggcga ccgccgagcc ggcgaacgcg ccgagcggcg gggtcccgat cgcccgggcg
     1981 cgctcctcgc cggcgatcac cagcgcggcg gccccgtcgt tgatcccgct ggcgttcccg
     2041 gcggtcaccg agccgccctc ccggaacgcc ggcgccagcg cggcgagctt ctccgtcgtg
     2101 gtgtccggcc ggggatgctc gtcggtgtcg acgccctcga ccgggacgat ctcgtcgtcg
     2161 aaccggccag ccgcctgggc cgcggcccat cgccgctgcg agcgcaacgc gaacgcgtcc
     2221 tgctcctcgc gcgacacggt ccagcgctcg gcgacgttct cgccggtctc gcccatcgtc
     2281 tcgagcggga acagggcctc catccgcggg ttcgggaacc gccagccgag cgtggtgtcg
     2341 aagatggtcc ggttcccgcg ggggaaggct cgttcgggct tcgccatcac cagcggcgcg
     2401 cggctcatcg actcgacgcc gccggccacg aacaggtcgc cgtccccggc caggatcgcg
     2461 tggcaggcgg acgcgaccgc cgacagcccg gacgcgcaca gccggttcac cgtgacgccg
     2521 gcgacggact ccgggagccc cgccagcagc cccgccatcc gggcgacgtt gcggttgtcc
     2581 tcgcccgcct ggttcgcgca gccgaggtag acgtcctcga tctcggccgg ctccacgccg
     2641 gcgcgctcca ccgccgccgc gatcgccacg gccgccaggt cgtcgggccg gacgccggac
     2701 agggccccgc cgtagcgccc gaccggcgtg cgcacggccg agaggaccac ggcgcgcgtc
     2761 acgccgaacc cccggagcga aggcgggcgc tggcctcgta gaccggcttg cgcgcgcggt
     2821 tcaggccacc gatcgggcgg atcgtcccgt ccgtcctcca cgggctgaac tcgagggcct
     2881 cgacgaggcg ctcgccctcc tggccctccg cgctcgccag gtcctgacgc gggatgacga
     2941 gctccgccag ggtctccggg cgcccgggct cgggccactc cacggttccg tcctcgatcg
     3001 gcgtggtgcg atcgtccacg tagtgctgca cctgcagctc gtagcgaacg gcctcgcgcc
     3061 gcaggcgctc gatcaggtcc cggcgcagct cgtcgtgtcc cagccttccg acacgctcgg
     3121 cgggcggcgc cacgggctgc agcttgaatt tcaccgcgta gggcccgatc gcgaacgggg
     3181 cgcggctgaa gaagggcacc gtggcgacgc tgtcgatcag ctgggcggac gcgtggcgca
     3241 gggcgcgcag cacgcgcacc ccctcccacg ggccgagctt gccgatcagc cccgggagcg
     3301 ccgtcagccg ccgcccgccg gccatcgcgt cggccgccgc catgaactgg cgggcgtcgc
     3361 gcgcgtggga cgtcggggtg tcggagagca ggaagtcctg gacgcggtcc ccccgctcgc
     3421 cgccggtctg cacccggatg gcgatcccgc gcaggtcctt gatcgagcgg ctggcggcga
     3481 cccccgacgc gctcgagtag cgcaccatcg ccgggtagga cgcccccggc aggatcaggc
     3541 cgacccgcag ctccgggcgc acgttctcgg ccaccacgaa ccgggccgtc gcgatcccgg
     3601 cgagcagctt cgcgtggaag ccccggcgga tctgctcgtc gccccggcgg ttccgtcgct
     3661 gcacgcgctg gatctgcgcg gcgtaggcgc gcaggaggtc ggcctcgcgc gcctcgctcc
     3721 cgccgaggta tcgctcgctc cacgacggga tgagcgtcgc ccgcggaacg gtgtcgtcgc
     3781 tcacgtctcg ggatcctccg ggaggaggct acccggtcgt ggttgccgcc acgaagcggc
     3841 gggcgatagc ctccgccgac atggccgggc cgccgcttcc ccccgggccg cgcacctggg
     3901 gcgtcctctc gaacgcgatc tcggccgccc ggttcgcccc cggctacctg cagcgcgtcg
     3961 cggcacgcta cggggacatc gcctgcatca aggcgggacc ggtccgcgtc tacctgctga
     4021 gcagccccga gctcgcgcag gacctgctcg agacgaacga ggcgcggttc gagaaggggc
     4081 gcgcggagca gcgggtcacg cgccgactgc tcggcgaagg ggtgctggcg agcgaggggg
     4141 agttccaccg gaggcagcac gcgctgatcg agcccctgct ccacggggcc gccctcgagc
     4201 gattcgcgcc aatcgtcgtc gagcgcgccg tgcgcatgca ggaagcgtgg caggacgggc
     4261 aggtggtcga cctgtgggat cggctgatcc gaacgatgac ggagatcatg gtggaggcgc
     4321 tgttcggggt ctccgtggac gctccggcgg gcgccgagct cctccagttg ctgaacgggg
     4381 ccgtcgacgc cgtcgaacgc ctcccgttcg ccgcgctccg gatcggtgag cgtctcccct
     4441 tcccgcggaa ccggcgcttc gaggaggtgc gtgcccggct cgacgccgtc ctgctcgggc
     4501 tggtggccga gcgccgcggc gaccgcaggc agaggggcga cctgctctcc tcactggccc
     4561 gcgcccgtca tcccgacggc gagccgatgc ccgaccggct ggtgcgggac gaggcgctga
     4621 cgctcttccg cgcggcgaag accaccggga ccgccctgtc ctggacctgg cacctgctgt
     4681 cgcggcaccc ggacccggag gcgcgcctgc tcgacgagat cgacacggtc gtcggcgacg
     4741 agcttccttc ggcatcggat ctcgcccgcc tcacgtactg ccgcagggtc ctggacgagg
     4801 ccctgcgcct gtttcccccc gcctggatct cggggcgccg ccccgtcgcc gagcacgaga
     4861 tcggcggcta cgtcatcccg gttggctcca ccgtcgccac cagcccgtgg gtgatccacc
     4921 gggacgcccg cgtccacccg gccccggagc gcttcgaccc cgaccggttc tcgccggagc
     4981 ggcgggcagg ctggcacccg ttcgcctact tcccgttcgg cggcggctcg cgcacctgcc
     5041 tgggcgacga gttcgccccg ctcgaggcgc tcatgctgct ggcgaccgtc ggccgccgat
     5101 ggcgtttgcg tccggtgccg gggcacgagg tgcgcccggc cgccaaggcc atcctcaggc
     5161 cgcgcggcgg gttaccggta gtcctgcagc gccgggcgca gccggtccgg tgaccccgcc
     5221 gcacgcctga gggcgcctga cgggagggcc agggtcacgc catggcgcag accacccaca
     5281 tgtccgccag atcatccggt ccgcccaatg ggatctctgg tttgtgtaga cggagaagat
     5341 ccacgcggca cgcccagccg gcatcgacgt tctgaccacg agtcgaacgc ttctccagcg
     5401 ccgcgccgca ggaacctccg cctgcgctcg ccgagctgac accaagggag ctcgaagtgc
     5461 tgcgcctgct cgcccgcggt ctctcgaacg cggagatcgc tgctgaactc gtcctctccg
     5521 agcacaccgc caagacccat gtcgctcaca tcctcagcaa gctcgacctg cgcgatcggg
     5581 tgcaggcggt tgtactcgca tacgagtccg ggatcgtccg cgccccgacc aggcagtaag
     5641 ccgcgccggc tgccatccgc agtaggcctg tcatccatgt tggtggcccc ggccgctgtc
     5701 ctgggcgctc ggcgtcaggc ttcagcggtt ggctacccgc tccagctttc gccggaaccg
     5761 gggcgccgcg ccggagcgga tcggcggccc gtgaccgaag cacgccacgt cgaagtcgag
     5821 ctcggccagc cggcggaagc tcgcccgggc ggcgacgtcg tcgtcgttca gcggggcgat
     5881 gtcgagcgtg cgcagcatgt tcgccgcggc gtcgccgacg aacagcaccg cgccctccgg
     5941 ccacaggaac gagacgtggc cggccgtgtg gccgggggta tggatgcagc gcagcccgcc
     6001 ggcggcgggc agctcctggc cgtcctcgac gagggcggcg accggcgctg cgtcggcccg
     6061 cgacgggccg cgcgccagga gacgcatgat ccagccggtc ggaccgtgcg cgtttccccg
     6121 cgggcgctcg gcgccgtccc ggacgatccg ggcgtcgcgc gcgtgggcgt agacggtcgc
     6181 gccggtggcg cgcacgacgg cggcgagctg cccggtgtgg tcagtgtggc agtgggtgac
     6241 ggcgacgtgg cggacgtcgg agggccgcag gcccagctgc cggagcggct cgaggacctc
     6301 gacggcgtcg cgtgggggcc cgcagtcgat cagggtcgcc tcgcggccgg cgatcaggta
     6361 ggcgttgttg ttcacgctcg ggaaccacat ccggcgaacg ccggcgacga cctccgtcga
     6421 gcgcacgctc aggccgtggc ggcccgccgg ctccgcaggc gccgagccat cacgtagccg
     6481 taggcgccga ggacgaacgc gctggccagg aacgcgaggt agaaccacca cccggcctcg
     6541 ttcgtctgca gcacctcgcc gaagcggacc agcgcgatca gctgcagcgc gccgagcacc
     6601 gggtagcccc acagcgccac cttgacccgg tcgaagtcgt tctccaggat cgcgtgcagg
     6661 gcgccgacgc cgaacgcgag cacccaggcc gagagcgcgc gcagcgcgag gggcgcgccg
     6721 atctcccacg gccacagcgg gccgcccacg ccgggggcaa cgaacgtcag gacgccgagc
     6781 gggatgaaga ccagagcatg ggcgccaagg accgtcttga cccacagcgg gagcgggtcg
     6841 gtgcgcggcg ggtccccgcc cgggaggcgg agctgctgga ccatctggac ggtcagctgg
     6901 atcgggtaga agccataggc cagcacccag aaccaggtga tgaccaccgc gaccccgccg
     6961 gcgtcgaagt ggaactcccc gaggtgcagg aacgtcgcgg cggtcatcag cggcgagaag
     7021 acgaacgcca ccgagatcga gatccggccg ttcgcccagt aacgctcccg ggacgccagg
     7081 atcgcgagga ccgtgctcgc ccagtagttg gcccccaggg tggccgccgt gagcggggtg
     7141 gcgatcgtcc aggcgaagta gtccgcggtc cgggtcgaga gcaggaagag caacgccccg
     7201 aggagcccgc gagtggccgg cccgaccacg ctcgcgcgca tggcgtcgga gaagggcttg
     7261 acggccccgg tctccacggc ctccgggctc acggcaggag actaccgtga gtgtccagcg
     7321 tgaccgagat accgcttccg ccagaagacc tgtggcgggt tggacgcggc ggcgacttcc
     7381 gactgatcgg ccgggacctc gtcgacctgc tcgtgtatca cgggctgcgg cccgaccatc
     7441 gcgtcctcga cgtcggctcc ggcatcgggc gcgtcgccgc cccgatgacg gcctacctca
     7501 ccacggggtc gtacgagggc ttcgacgtcg accgggagat ggtggcgtgg tgccgccgga
     7561 acatcaccat gcggttccgc aacttccggt tccaggtggc cgacctgtac aacaagcagt
     7621 acaaccccac cggaaggcag cgcgccgaga cctaccggtt cccgtacgag gatgcgtcgt
     7681 tcgactacgt gctgctcacg tcggtgttca cgcacatgcc gccgaaggag gtcgaccact
     7741 acctgtccga gatctcccgc gtgctggtcc ccggggggat cgcgtccatc acctggttcc
     7801 tggtcgacca ggactccagg aagatcctga tcgggcgtga tcgggggccc gatttccgct
     7861 tccggcggcg aaagcaccgc atcgccaacc gccggaaccc tgcggcggcg gtcgcgcacg
     7921 acctgcgcaa cgtgcgagcc atgtaccggc gaaacggcct gtggattcga gagcttcgcc
     7981 gcgggtcatg gcgcggatcc gccggcggcc cgggagcgac ccatcaggat ctgatcgtgg
     8041 ccacccgtcg ctgacctcat caccgggcga gcggtaggct gcgaagcgga cggagcgaga
     8101 cgggggcgag gatggagttc ggggtgtgct tctcggccga cccgccgacc cggcgctggg
     8161 tggacctctc ggcgctcgcc gagcggcacg ggttcgacta cgtctggatg tgggactccc
     8221 acgtcctgtg gcgcgaggtc taccccctct tcacgctgat cggggaggcc accgagcgcg
     8281 tccggatcgg tccgctggtc accaacccgg tcacccgcga cccgaccgtg acggcgagcg
     8341 tcctggctac gctcgacgag ctgaacggcg gccgcatggt gatggggatc ggccgcggcg
     8401 acagcgcgcg gcgagtgatc gggcagaccc cggtcggcgt cgagcgcatg gaatgggcct
     8461 ccggcctgat ccgggacctg gtggcgggac ggcaggtcga ctacgagggc gcccccgtca
     8521 agctcgagtg ggcgcactgg gacctgccgg tgtggatggc cgcctacggg ccgaaggccc
     8581 tgcgcgcggt cggccgcgtc gccgacgggc tgatcatgca gctcgccgat ccgttcatca
     8641 tcgagtggtc gctcgcctac gtccgccagg gggccgagga ggcggggcgc gaccccggcg
     8701 cggtgaagat catgagcgcg gcgccga
//
LOCUS       DASPWO010000231         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_34062, whole genome shotgun sequence.
ACCESSION   DASPWO010000231 DASPWO010000000
VERSION     DASPWO010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34062"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VEO00_05195"
     CDS             <1..731
                     /locus_tag="VEO00_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HYU57427.1"
                     /translation="GREAMVPHNVPMVITGAAILWFGWFGFNAGSALGANGLAASAFA
                     NTHLATAAAVLGWLIPERAKHGKATTIGAATGAVAGLVAITPAAGFVEPWAALVIGFV
                     AGAVCWKAVDLKTRIGYDDSLDVVGVHFVGGVVGALLTGVFASAAVNAFSGSLAQFGK
                     QALAVGVTIAFSFLMTLLILKVVDVLLGGVRVPEEEEVTGLDLSQHSEVGYTFQDRGG
                     SLAAGHDEPAGAEPVGAVDREGAR"
     gene            728..1066
                     /locus_tag="VEO00_05200"
     CDS             728..1066
                     /locus_tag="VEO00_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HYU57428.1"
                     /translation="MKLVVAIVKPFKLDEVKEALRAAGVQGLTATEAQGFGRQRGHTE
                     VYRGAEYRVDFVPKVKVEVLCDDDQVQGVVDAIMKSARTGKIGDGKIFVLPAEQAYRI
                     RTGEMGPDAI"
     gene            1185..>2416
                     /locus_tag="VEO00_05205"
     CDS             1185..>2416
                     /locus_tag="VEO00_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57429.1"
                     /translation="MDEALRELLERAVAGTARTGPLALVALGGYGRGELCPRSDVDLM
                     VLHGERRPERVRVVAERVFYPLWDAGIALGHAVRTVKQAAAEAEAGVETATALLEARH
                     LAGEPALVEQLHTALAATLGADRAGFLERVAAADAERHVRFGSAAHLMEPDVKECAGG
                     LRDVHSVGWAARAIGLPGPEALEAEGLLRQAEREALEAGEEFLVRVRSALHLEAGRRS
                     DRLVLDLQPRLADAFGFGATGGLDAPDALMRALFDHARHVEHVRALAFERARAGVAVR
                     GAEETIAPPLVAGPEGVMAAFAEWAEGRPAPAPTTLDALEETPFEDGSPWSAATLDAF
                     LRILRAGGAGARALEGMDRVGLLSRFLPEWADVRCRPQRDPYHTFTVDVHLTRTVAEA
                     AALLAGRSEDPAVRAAAAA"
BASE COUNT          282 a          824 c          987 g          323 t
ORIGIN      
        1 tcggcaggga ggcgatggtc ccgcacaacg tgccgatggt gatcaccggc gcggccatcc
       61 tctggttcgg gtggttcggc ttcaacgcgg gatcggcgct cggggcgaac ggcctggcgg
      121 cgtcggcgtt cgccaatacc cacctggcca ccgcggccgc cgtcctcggc tggctgatcc
      181 ccgagcgggc caagcacggg aaggcgacca cgatcggcgc ggccaccggt gcggtggccg
      241 ggctggtggc gatcaccccg gcggccgggt tcgtcgagcc gtgggcggcc ctcgtgatcg
      301 ggttcgtcgc cggggcggtc tgctggaagg cggtggatct caagacgcgg atcggctacg
      361 acgattcgct cgacgtcgtc ggcgtgcact tcgtcggcgg ggtcgtcggc gcgctcctga
      421 ccggcgtgtt cgccagcgcg gcggtgaacg ccttctccgg gagcctggcg cagttcggca
      481 agcaggccct tgccgtcggc gtgacgatcg ccttctcgtt cctgatgacg ctcctgatct
      541 tgaaggtggt cgacgtcctg ctcggcgggg tgcgggtgcc ggaggaggag gaggtcacgg
      601 gactcgacct cagccagcat tccgaggtgg gctacacgtt ccaggaccgg ggcggatcgc
      661 tcgcggccgg gcacgatgag ccggcgggcg ccgagcccgt cggggcggtc gatcgggagg
      721 gggcgcgatg aagctggtcg tggcgatcgt caagccgttc aagctcgacg aggtcaagga
      781 ggcgcttcgc gcggcaggcg tgcagggctt gaccgcgacc gaggcgcagg ggttcggccg
      841 gcagcggggg cacaccgagg tctatcgcgg cgccgagtac cgggtcgact tcgtgccgaa
      901 ggtgaaggtc gaggtgctct gcgacgacga ccaggtccag ggagtcgtcg atgcgatcat
      961 gaagtcggcc cggaccggca agatcggcga cgggaagatc ttcgtcctgc cggccgagca
     1021 ggcttaccgg atccgcaccg gggagatggg gcccgacgcg atctgaggtc gccgttggga
     1081 acggggggcc cgccgcccgg ctgatcgagg ggctgcgggc gctcgatcgc gcctacacga
     1141 gcggccacca cgggcgctgg tcggcgcgcc ggcgtgccgc gctcgtggac gaggccctgc
     1201 gcgagctgct cgagcgggcg gtggccggga ccgcccggac cgggccgctc gccctcgtcg
     1261 ccctcggcgg gtacggacgc ggtgagctct gcccccgttc cgacgtggac ctgatggtgc
     1321 tgcacggcga gcgccgcccc gagcgggtcc gggtggtggc cgagcgggtg ttctacccgc
     1381 tgtgggacgc cgggatcgcc ctcggccacg cggtgcgcac ggtgaaacag gccgccgccg
     1441 aggcggaggc cggcgtggag accgcgacgg cgctcctcga ggcgcgccac ctcgccggcg
     1501 agccggccct ggtcgagcag ctgcacaccg cgctggccgc cacgctgggg gccgaccggg
     1561 cagggttcct ggaacgggtc gccgcggccg atgccgagcg gcacgtgcgg ttcgggtcgg
     1621 cggcgcacct gatggagccc gacgtgaagg agtgcgccgg cgggcttcgc gacgtccact
     1681 ccgtcggctg ggcggcgcgg gccatcggcc tgcccggacc ggaggcgctc gaggcggagg
     1741 gcctgttgcg gcaggccgag cgggaggcgc tggaggccgg cgaggagttc ctggttcgcg
     1801 tgcggagcgc cttacacctg gaggccggca ggcgctccga ccggctcgtg ctcgacctgc
     1861 agccgcgcct ggccgatgcg ttcgggttcg gggccaccgg cggcctcgac gcaccggacg
     1921 cgctgatgcg cgcgctgttc gaccacgccc gccacgtcga gcacgtccgc gccctggcgt
     1981 tcgagcgcgc gcgggcgggg gtggccgtcc ggggagcgga ggagacgatc gcgccgccac
     2041 tcgtcgccgg gcccgagggg gtgatggcgg cgttcgccga gtgggccgag gggcgcccgg
     2101 cgcccgcgcc gacgacgctc gacgccctgg aggagacccc cttcgaggac gggtcaccgt
     2161 ggtcggcggc caccctggac gcgttcctgc gcatcctgcg ggccggcggc gccggggcgc
     2221 gcgcgctgga gggcatggac cgcgtcggcc tgctgtcgcg cttcctgccc gagtgggccg
     2281 acgtgcgctg ccggccgcag cgggacccgt accacacgtt caccgtggac gtgcacctga
     2341 cgcggacggt cgccgaggcg gcggccctgc tcgccggccg gtccgaggac cccgcggtgc
     2401 gcgcggcggc ggccgc
//
LOCUS       DASPWO010000232         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_34148, whole genome shotgun sequence.
ACCESSION   DASPWO010000232 DASPWO010000000
VERSION     DASPWO010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34148"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VEO00_05210"
     CDS             <1..499
                     /locus_tag="VEO00_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HYU57430.1"
                     /translation="ARFVGLPFPGGPEIDRLAQRGNPAAIAFPRALAGSGDFDFSLSG
                     LKTAVVRHVRAERDAGREVDLADLAASFQEAVVDVQVDKTMGAAEERKVSTVLLAGGV
                     VANTRLRERMAEAAEANGLALRYPSPELCTDNAAMIACAGFFRLAKGERTSLEVGADA
                     GLPLA"
     gene            558..1508
                     /locus_tag="VEO00_05215"
     CDS             558..1508
                     /locus_tag="VEO00_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYU57431.1"
                     /translation="METVVVTGAAGFIGSHLVDRLLAEEKQVVGIDDLSTGRIANLSE
                     ARQYGHAFTFYNLDIRTEGLGQLLERHRPDVVMHLAAQAGVRPSLSTPALDASINVMG
                     LLNLLEASVGCGARKVVYASSGGTIYGEPRRLPVKETALGGSHPSSPYGVSKRVGGDY
                     LRYYHRYRGLDYTALALGNVYGPRQDPYGEAGVIAIFIGRMLAGEPVTIFGDGNQTRD
                     YVFVDDTVHAFALAAGEAGSGRLINIGTALETSVNGLFRMLADVIGYGLEPALGPLPA
                     GEPRRIALDNALAASELGWRPWTHLEDGLKETVAYLRDSN"
     gene            1589..>2210
                     /locus_tag="VEO00_05220"
     CDS             1589..>2210
                     /locus_tag="VEO00_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU57432.1"
                     /translation="MGASARTIVVVEDEATIATAVAARLRKEGFRVEVAGDGPSGVEV
                     VERLRPDLVILDLMLPGFDGLEVCRRIQRRRAVAVIMLTARDSETDVLVGLGVGADDY
                     VTKPFSPRELVARVQAVLRRAERAPAPEREPVRAGDVEVDPRTRRVSRAGRQVHLTPI
                     EFDLLLELAARPGEVFDRERLLADVWGYRDGSGARTVDSHVRALRKK"
BASE COUNT          294 a          792 c          809 g          315 t
ORIGIN      
        1 ggcccgcttc gtcggcttgc cgttcccggg cggcccggag atcgaccggc tggcccaacg
       61 tggcaacccg gcggcgatcg ccttcccccg cgccctggcc ggctccggcg acttcgactt
      121 ctcgctgtcg ggcctgaaga ccgcggtggt gcgccacgtt cgcgccgaac gcgacgccgg
      181 ccgggaggtg gacctggccg acctggcggc ctcgttccag gaggcggtgg tcgacgtcca
      241 ggtcgacaag acgatggggg cggcggaaga gcggaaggtt tccaccgtgc tgctcgcggg
      301 cggcgtggtg gcgaacacgc gcctgcgcga gcggatggcc gaggccgcgg aggcgaacgg
      361 cttggcgctg cggtacccgt cgccggaact ctgcaccgac aacgcggcga tgatcgcctg
      421 cgcgggcttc ttccggctgg ccaaggggga gcgcacctcg ctcgaggtcg gagccgacgc
      481 cggcctcccc cttgcctgag cggctgctgc tcggggaaca cggggggcgg agctccccgg
      541 gcgggtagca ttcgggcgtg gagaccgtcg tggtcaccgg ggcggccggg ttcatcggct
      601 cccacctggt ggaccgccta ctggccgagg agaagcaggt cgtggggatc gacgacctgt
      661 ccaccggccg gatcgccaac ctctccgagg cgcgccagta cgggcacgcg ttcacgttct
      721 acaacctcga catccggacc gagggactgg gacagctgct cgagcgccat cgacccgacg
      781 tggtgatgca cctcgcggcg caggccggag tgcgcccgtc gctctcgacc cccgccctcg
      841 acgcgagcat caacgtgatg gggctcctga acctgctgga ggcgtcggtc gggtgcggcg
      901 cgcgcaaggt cgtctacgcc tcgtccggcg gcacgatcta cggcgagccg cgccggctgc
      961 cggtcaagga gaccgcgctc ggcgggtcgc atccctcgtc gccctacggg gtatcgaagc
     1021 gggtcggcgg cgactacctc cgctattacc accgctaccg cggtctcgac tacacggcgc
     1081 tcgctctggg caacgtctac gggccgcgac aggacccgta cggcgaggcc ggcgtgatcg
     1141 ccatcttcat cgggaggatg cttgccggcg agccggtgac gatcttcggc gacggcaacc
     1201 agacccgcga ctacgtgttc gtcgacgaca ccgtgcacgc gttcgccctc gccgccgggg
     1261 aggcgggctc cgggcggctg atcaacatcg gcaccgcgct cgagacctcg gtcaacggcc
     1321 tgttccggat gctggccgac gtcatcggct acggcctcga gccggcgctc gggccgctcc
     1381 ccgccggtga gccgcggcgc atcgcgctcg acaacgccct cgccgcgtcc gagctcggct
     1441 ggcgcccgtg gacgcatctg gaggacggcc tgaaggagac ggtggcctac ctgcgcgatt
     1501 cgaactgaac gggcgcgcat cggacctgca cggaatctcc acagcgatgc gaccgaccgc
     1561 gcacgtgcgg atgcctagac ttcatggtat gggcgccagc gcgcgcacga tcgtcgtcgt
     1621 cgaggacgag gcgaccatcg ccaccgccgt cgccgcccgg ctgcgcaagg aggggttccg
     1681 ggtggaggtc gccggcgacg gcccgtcggg cgtcgaggtc gtcgagcgcc tgcggccgga
     1741 cctggtgatc ctcgacctga tgctcccggg gttcgacggg ctcgaggtct gccggcggat
     1801 ccaacggcgc cgggcggtgg cggtgatcat gctcaccgcg cgcgactctg agaccgacgt
     1861 cctggtcggc ctcggcgtcg gggccgacga ctacgtgacg aagcccttct cgccgcgcga
     1921 gctcgtcgca cgggtgcagg cggtcctgcg ccgcgccgag cgcgcacccg cgcccgagcg
     1981 cgagccggtc cgcgccggcg acgtcgaggt ggatccgcgc acccgccgcg tcagccgggc
     2041 cggtcgccag gttcacctga ccccgatcga gttcgacctc ctgctcgagc tcgccgctcg
     2101 cccgggcgag gtcttcgacc gcgagcggct gctcgccgac gtctggggct accgggacgg
     2161 gtccggggcg cggaccgtcg attcccacgt tcgggcatta cgcaagaagc
//
LOCUS       DASPWO010000233         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_545518, whole genome shotgun sequence.
ACCESSION   DASPWO010000233 DASPWO010000000
VERSION     DASPWO010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545518"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VEO00_05225"
     CDS             <1..370
                     /locus_tag="VEO00_05225"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HYU57433.1"
                     /translation="AVLVSMLALAAVGVETAKAQMASRTPESYLRVESTVEPSKSGRQ
                     RITGYVYNERDLRAANVQLLVEALDASGQVVGSTPAWVYGEVPPRNRSYFEVPLSLSG
                     SSYRVTVRSVDWRGYGAGGG"
     gene            complement(743..880)
                     /locus_tag="VEO00_05230"
     CDS             complement(743..880)
                     /locus_tag="VEO00_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57434.1"
                     /translation="MFRRLEGDGYRAHYMMAFGSLDDMLKGRDALVRSAGPVEPRGLP
                     S"
     gene            1167..1472
                     /locus_tag="VEO00_05235"
     CDS             1167..1472
                     /locus_tag="VEO00_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57435.1"
                     /translation="MRSTAVGLLMVVAASGCATAHNTPAQDLSWERWRACDHFTTIAL
                     DRVDVDGRLVVKGYQYEAALFTACVREAAADQVRRGVPAEPSVAVLVKLYGCLGGAM"
     gene            1499..1888
                     /locus_tag="VEO00_05240"
     CDS             1499..1888
                     /locus_tag="VEO00_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57436.1"
                     /translation="MTIIHRTIGAVLLAGFMWQDAGSARAERSTVVGNAGTITLDWQA
                     TQDRAGRPLIEGHVITYGGKSGYCRPRLLVETLDAQGVVTASNVGFIPGYVGGFGDVY
                     FQERVRAPGPAYRVSIASWDKCAGGGQ"
     gene            complement(1916..>2128)
                     /locus_tag="VEO00_05245"
     CDS             complement(1916..>2128)
                     /locus_tag="VEO00_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYU57437.1"
                     /translation="SMPVLFLHGEYDYTCETVMSPLAEPMRRDCRNLTEVVVASGHWM
                     AQEKPVAVNAALAKWLAVQFPEIWPV"
BASE COUNT          313 a          714 c          777 g          324 t
ORIGIN      
        1 ggcggtgctc gtctcgatgc tggctctcgc cgccgtcggc gtggagacgg ccaaggccca
       61 gatggcgtcg cgaacgcccg agtcctacct gcgagtcgaa tcgacggtgg agccgtcgaa
      121 gagcggacgc caacgcatca cgggctacgt ctacaatgag cgagacctca gggccgccaa
      181 tgtccagctc ctcgtagagg ccctggacgc ctccgggcaa gtggtgggct caacgcccgc
      241 ctgggtctac ggcgaggtgc cgcccaggaa ccgctcgtac ttcgaggtcc cgctctcgct
      301 gagtggctct agctaccggg tgacggtgcg ctccgtcgac tggcgcggct acggcgccgg
      361 cggcggctaa ggtcgacgct cgggacgagg tcgctccacg cgccggcgcc gagtggcgga
      421 ggagagacga gcagcccggg cgctacgcgg cgacgcccct gcgcgtatcc acgaatcacg
      481 acgtgcagtc ccgaagatca gcccgccggg ccgcaacagg tcgattcgac taccgggctc
      541 ggctgcggat gctcgcttcg ggcgactgaa ggacggtgaa ctatcgggcg tggtcgcgtt
      601 cggcggatct gctcgcggcg cgcgatcgga tgtcttcagc cggcgacgca ttgccgtgtt
      661 cggtcgacgg ccgctccgtg acgcgccgga atctcgacgt tggtgatgat cttccccgcc
      721 gccgggtctc gcccgggagg cgtcacgacg gcagcccccg gggctcgacg gggcccgcgg
      781 accgcacgag cgcgtctcgg cccttcagca tgtcatcgag cgagccgaac gccatcatgt
      841 agtgagcgcg atagccgtcg ccctcgagcc gccggaacat gcggccgaag tcggtcgttc
      901 cctcgcccgg cctcaggtgt tgctcgcggt cccggccgtt gtcggcgagt cgacctcgcc
      961 gcaccgatcg aggcccatgg cgtcgaggaa gccctctcct tgttcaatcc ccggagaggc
     1021 gagatggggc ggacgccgcc gtccgagacc acgcccgcaa tcggcgcgcc tcggcgtgca
     1081 aggggtgtag gctggggggc agcgggcagg gcgatcggcc ggcgcctgcc cgccgggtga
     1141 cctccgggga gaaaggcagg agaatcatgc gatcgaccgc ggtcggcctc ctcatggtgg
     1201 tggcggcttc tggatgtgcc accgctcaca acacgcccgc ccaggacctc tcgtgggagc
     1261 gctggagggc gtgcgatcac ttcacaacga tcgccctcga ccgggtcgat gtggacggcc
     1321 ggctcgtggt caaggggtac cagtacgagg ccgcgctctt cacggcgtgc gtgcgcgagg
     1381 cggcggccga tcaggtccgc cggggcgtcc cggccgagcc atcggtggcc gtactggtga
     1441 agctctacgg gtgcctgggc ggggcgatgt gagtcgggga gagcgcggag gaatcgcgat
     1501 gacgataatt caccggacga tcggagcggt gctcctggcc ggcttcatgt ggcaggacgc
     1561 cggctcggcg cgggccgagc gcagcaccgt cgtgggtaat gctggcacca tcaccctcga
     1621 ctggcaggcg acccaggaca gggccggccg tcccctcatc gagggtcacg tcatcaccta
     1681 cgggggcaag tccggctact gccggccccg gctcctggtg gagacactcg acgcccaggg
     1741 tgtggtcacc gcgtccaacg tgggcttcat ccccggctac gtgggcggct tcggcgacgt
     1801 ctatttccag gagcgggtac gcgcgcccgg tcccgcctat cgcgtcagca tcgcgtcctg
     1861 ggacaagtgc gccggggggg gccagtaggc gcgtgccggg cgcgcgggtg gccgatcaga
     1921 cgggccagat ctccgggaac tggacggcca gccatttggc gagggcggcg ttgacggcca
     1981 cgggcttctc ctgtgccatc cagtgccccg acgctacgac cacctcggtc aggttgcgac
     2041 agtcccgtcg catgggctcc gccagggggg acatcacggt ctcgcaggtg tagtcgtact
     2101 ccccgtgcag gaacagcacc ggcatgga
//
LOCUS       DASPWO010000234         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_116495, whole genome shotgun sequence.
ACCESSION   DASPWO010000234 DASPWO010000000
VERSION     DASPWO010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116495"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VEO00_05250"
     CDS             <1..1277
                     /locus_tag="VEO00_05250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU57438.1"
                     /translation="DALKRADALAGLEPAGIHVHIGSQVLEVEPYLAALEVVLDLVAE
                     ARRGLELALSTLDLGGGFGVTYTDERPPEPAVLGALVHQRLAAAARERGIAPPDLVVE
                     PGRAIVANPVLTLYRVGTIKEVPGVRTYVSVDGGMSDNIRPALYGSRYAFALANRPGA
                     DERTPVTVAGRHSESGDILGTDVPVPADIERGDLLAVAATGAYAYAMASTYNRAGRPA
                     AVALGDGEARLILRREEDGDLDRLEMSGGPLPEAAPPAGVVVRPARPRDARSFLALWT
                     GVVAERRFVRTEQVRQTLRAERRRFKHSWTEQGTHLVAVEGRRVVGQLTAARDANAVN
                     HHVATLGIAIAPDRRGRGIGAALMSTCVRWAQQVGVEKLALSVYPENRAALALYAKFG
                     FVEEGRLSGHSKKSYGYEDEIVMGRWLPGSRS"
     gene            1274..2560
                     /locus_tag="VEO00_05255"
     CDS             1274..2560
                     /locus_tag="VEO00_05255"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HYU57439.1"
                     /translation="MTRSVRVGLLGCGNVGTALVRLLAEVADVIEARAGCRVEVTRVA
                     VRSLSKDRDVPLPPEALTADAASVVADPDVDVVVEVIGGIEPARSLILSAFAAGKHVV
                     TANKELLSTLGRELFDAAEAARVDLYFEGAVGGGIPLIHPLKESLAAEQVARLTGIVN
                     GTTNYVLTRMTEEGLSFSDAIAQAQGLGYAERDPSADVEGYDAAAKCAILASIAFNAR
                     VVAGDVYREGIGSVTREDIDFAGRLGYVVKLLAIAELDDGEIAVRVHPAMIPGDHPLA
                     AVRDSFNAVFVEGTHVGELMFYGRGAGGAPTATAVAGDVVDVARNLVAGGRGVGCTCY
                     EDRAIRPMDRMESQYYLLLDVVDRPGVLAQVAGAFGANRVSIRSVWQEGTGEDALLVI
                     ITHRGNEGALQSTVHGLRALDVVNEVRSVMRVEGAE"
     gene            2566..2715
                     /locus_tag="VEO00_05260"
                     /pseudo
     CDS             2566..2715
                     /locus_tag="VEO00_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151374.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
BASE COUNT          350 a          957 c         1041 g          381 t
ORIGIN      
        1 tcgacgcgct gaagcgcgcc gacgcgctgg ccggcctcga gcccgccggg atccacgtgc
       61 acatcgggtc gcaggtcctc gaggtggagc cgtacctggc ggcgctggag gtcgtgctcg
      121 acctggtcgc cgaggcccgc cgcggcctcg agctcgcgct ctcgacactc gatctcggcg
      181 ggggcttcgg ggtgacctac accgacgagc gtcctcccga gccggcggtg ctcggcgccc
      241 tcgtgcacca gcggctggcc gccgcggcgc gcgaacgggg gatcgcccct ccggacctcg
      301 tcgtcgagcc cggacgggcg atcgtggcga acccggtcct gacgctctac cgggtcggga
      361 cgatcaagga ggtccccggc gtgcggacct acgtctcggt ggacggcggc atgtcggaca
      421 acatccggcc ggcgctgtac gggtcgcgct acgcgttcgc cctggcgaac cggcccggcg
      481 ccgacgagcg gacgccggtg accgtggccg gccgccacag cgagtctggg gacatcctgg
      541 ggaccgacgt gcccgtgccg gccgacatcg agcgcggcga cctgctggcg gtggcggcca
      601 ccggcgcgta cgcgtacgcc atggcgagca cgtacaaccg cgccggccgg ccggcggcgg
      661 tcgccctcgg tgacggcgag gcccggctga tcctgcggcg cgaggaggac ggggacctcg
      721 accggctcga gatgtccggc ggcccgctgc ccgaggccgc gccgccggcc ggcgtggtcg
      781 tccgcccggc ccgtccccgc gacgcgcggt cgttcctggc actgtggacc ggggtggtcg
      841 ccgagcggcg cttcgtccgg accgagcagg tccgccagac gctccgggcc gagcgccggc
      901 ggttcaaaca ctcctggacc gagcagggga cgcacctcgt cgccgtggaa ggccggcggg
      961 tggtcgggca gctgacggcg gcccgcgacg cgaacgcggt caaccaccac gtggcgacgc
     1021 tcggcatcgc catcgctccc gatcgccgcg ggcgggggat cggggcggcg ctgatgtcca
     1081 cgtgcgtccg ctgggcgcag caggtgggcg tggagaagct cgcgctgtcg gtctacccgg
     1141 agaaccgggc cgcgctggcc ctgtacgcca agttcggctt cgtcgaggag ggccgcctgt
     1201 cgggccactc caagaagtcc tacggctacg aggacgagat cgtcatgggc cgctggctcc
     1261 ccgggagtcg gtcgtgaccc gctcggtccg cgtcggcctg ttgggctgcg gcaacgtcgg
     1321 gaccgccctc gtgcgcctgc tggccgaggt cgccgacgtg atcgaagccc gcgccggctg
     1381 tcgtgtggag gtgacgcgcg tcgcggtgcg ctcgctctcg aaggaccgcg acgtgcccct
     1441 gccgccggag gcgctgacgg cggacgccgc gagcgtggtg gccgatcccg acgtcgacgt
     1501 cgtggtcgag gtgatcggcg ggatcgagcc cgcccgctca ctcatcctct ccgcgttcgc
     1561 cgccgggaag cacgtcgtca ccgcgaacaa ggagttgctc tccacgctcg gacgcgagct
     1621 gttcgacgcg gccgaggccg cgcgcgtcga cctgtacttc gagggtgccg tggggggcgg
     1681 gatcccgctg atccatccgc tgaaggagtc cctggccgcc gagcaggtcg cccggctgac
     1741 cggcatcgtc aatgggacca cgaactacgt cctgacccgg atgaccgagg agggcctctc
     1801 gttctccgac gcgatcgcgc aggcgcaggg cctcggctac gcggagcgcg acccgagcgc
     1861 cgacgtggaa ggctacgacg ccgcggcgaa gtgcgccatc ctcgcgtcga tcgccttcaa
     1921 cgcccgggtc gtggccggcg acgtctaccg cgaggggatc ggctcggtca cccgggagga
     1981 catcgatttc gccggtcggc tcgggtacgt ggtcaagctc ttggccatcg cggagctgga
     2041 cgacggcgag atcgccgttc gcgtccaccc ggcgatgatc cccggcgacc atccgctggc
     2101 cgccgtgcgc gactccttca acgcggtgtt cgtcgaggga acgcacgtcg gtgagctcat
     2161 gttctacggc cgtggcgcgg gcggcgcgcc gaccgccacc gcggtcgccg gagacgtcgt
     2221 ggacgttgcg cggaacctgg tggccggcgg gcgcggcgtg gggtgcacct gctacgagga
     2281 ccgggccatc cgtccgatgg accgcatgga gagccagtac tacctgctcc tcgacgtggt
     2341 cgaccgtccc ggcgtgctgg cgcaggtggc cggggccttc ggcgccaacc gcgtttcgat
     2401 cagaagcgtg tggcaggagg ggaccggcga ggatgcgctc ctggtgatca tcacgcatcg
     2461 cggcaacgag ggcgcgctgc agagcacggt gcacggcctg cgcgcgctcg acgtcgtgaa
     2521 cgaggtccgc agcgtcatgc gcgtcgaagg cgcggagtag gtccaatggc ccggccgtgg
     2581 cggggggtga tcgaggagta ccgggatcgc ctcccggtca ccgacgccac ccccgtgatc
     2641 accctgcagg aggggggcac gccgctcgtg cgctcagcgt cgctgtccga ggagacgggg
     2701 cgttccgtgt ggctgtagtt cgagggcgc
//
LOCUS       DASPWO010000235         9570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1055663, whole genome shotgun sequence.
ACCESSION   DASPWO010000235 DASPWO010000000
VERSION     DASPWO010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055663"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VEO00_05265"
     CDS             <1..401
                     /locus_tag="VEO00_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57440.1"
                     /translation="EAPPGPAGVGTEFFSTGTDGKVARWSDRSVVTEATRPAVFEFVT
                     DGLRRGKPGSRPWRATAVHRYEIVPAGEGCRVTYTEDLTRLTGAPRILFSAPMSRIVF
                     LIAARYMRRGFDNLLALAEERAGEPAEGRV"
     gene            401..613
                     /locus_tag="VEO00_05270"
     CDS             401..613
                     /locus_tag="VEO00_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU57441.1"
                     /translation="MGSLARFPVYAVLPASDFERARAWYREKLELTPSEELPGNAWYR
                     CADGTWFILTQTPNARTARTRRPGSR"
     gene            610..786
                     /locus_tag="VEO00_05275"
     CDS             610..786
                     /locus_tag="VEO00_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57442.1"
                     /translation="MKEIESAMEGLRSRGVVFEEYDFGEMGGTENGLMKMGSTKAAWF
                     KDSEGNIIELSQTG"
     gene            complement(825..1844)
                     /locus_tag="VEO00_05280"
     CDS             complement(825..1844)
                     /locus_tag="VEO00_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYU57443.1"
                     /translation="MPLKTLRLGTTGPRVSAIGLGCMGMSGAYGPVDEAESIATIHAA
                     LDAGIDLLDTGDYYGMGHNELLIREALRGRDRDAVAISVKFGALRAPDGAFIGVDGRP
                     VAVKSFLAYTLRRLDTDHVDIYRLGRVDPAVPIEDTVGAIAEMIAAGHVRHVGLSEAG
                     AETVRRANAVHHVADLQIEYSLLSRGIEAEILPTCRELGVGVTAYGVLARGLLAGAWS
                     RDRALSPGDIRARMPRFRPENLDRNLALVEALRAIADQKGATVAQIAIGWLLTRGDDI
                     VPLVGTTRRARLAEALAALELQLTDADLEAIERAAPPGAAAGDRYDERQMAMLDSERS
                     AAPEA"
     gene            complement(1870..2673)
                     /gene="amrB"
                     /locus_tag="VEO00_05285"
     CDS             complement(1870..2673)
                     /gene="amrB"
                     /locus_tag="VEO00_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HYU57444.1"
                     /translation="MTPPVRRPVAGGTFYPGDPRSLAAEVDRLLSEAFPPSGVPDPLG
                     LIVPHAGYRYSGPVAATAYALLSRAASRPDRVALLGPAHFVPLRGAAVSAARAWATPL
                     GTVDVDAELAGVAAACGAVRDEEPHDQDHALEVQLPFLQRVLASALRVLPVAVGDADP
                     GEIAALIGALADAGALVLVSTDLSHYRDHRMAQAIDGRTAAAVVARDAFAIAADAACG
                     VHALRGLVAHARHRGLAITPLDLRTSGDVTGDRGSVVGYGAFAVVSGDH"
     gene            complement(2670..3737)
                     /gene="amrS"
                     /locus_tag="VEO00_05290"
     CDS             complement(2670..3737)
                     /gene="amrS"
                     /locus_tag="VEO00_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HYU57445.1"
                     /translation="MYPTRHWHRLDDGRVQCDVCPRACRLHEGQRGLCFVRMRQDDRI
                     VLTTYGRSSGFCVDPIEKKPLNHFLPGTSVLSFGTAGCNLACRFCQNWDISKSREIDT
                     LADAASPEDLAAAAERLGCRSVAFTYNDPVIFMEYAMDVADACRARGIRAVAVTAGYM
                     CADPRADFYRHMDAANVDLKAFTDGFYHDVCAAHLEPVKETLEYLVHETDVWVEVTNL
                     LIPGLNDSDDEIDDMTRWAVERLGPDVPMHFTAFHPDWKMRDRPPTPPATLSRARRLA
                     MANGLRHAYTGNAHDTEGQSTYCTGCGRMLIERDWYRLGDWHLTADGRCASCGTRCAG
                     VFESEPGRWGPRRLPVLLGGG"
     gene            3842..5050
                     /locus_tag="VEO00_05295"
     CDS             3842..5050
                     /locus_tag="VEO00_05295"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYU57446.1"
                     /translation="MAPRVGASSEPPPSPEAGAPRSTPGGARRYVATAVALLLTAVVV
                     ATTVYLVDRRELSRVRSDLADVRRDLEGLNDRVAGLTDERDRLRERVDALSRLAGDPL
                     DDGDYTVRVRAAVHGRPDRLGVSVLTGPAGGTGWRLLEVAAGADVRLAAVPGLDPTVM
                     NFVRFARMFNGGRPEDDALHTVRFRIALAGGRVTAIKSLDAVAPAALEPGATGPFVEA
                     LQERLAALRFDVGPRDGTFGDDTSHAVVAFQKVNGLPRDGTVTRATWEAMARPVIPAA
                     RNRSSGETIEVDLTRQVLYLVRDGRIVRIVDASTGGGYAFTTKGVQKVAITVTGEFRI
                     YWKIEGWYQSSLGPMYRSSFYYRGFAIHGYGSVPPYPASHGCVRVTFAAVDRLWPHLF
                     EGMPVSVYRG"
     gene            5164..6153
                     /locus_tag="VEO00_05300"
     CDS             5164..6153
                     /locus_tag="VEO00_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU57447.1"
                     /translation="MNEPAIVAEGLVKRFGDVRALDGVDLEVDQGSVLGLLGPNGAGK
                     TTAIRILSTLLSPDAGSARVGGFDVVHQPVQVRRLIGLTGQYAAVDELLSGHENLYMI
                     GRLLGLSKPAARRRASELLANFELSEAAVKVVKAYSGGMRRRLDLAASLVGRPRFLYL
                     DEPTTGLDPRSRRQLWNMIRGLVREGATVLLTTQYLDEADSLAHRIAVIDHGHVIAVG
                     TSDELKAKTGGLVIEARIEARYLDAVARILAEISGNEAAIDREANVITVPVGDPGALP
                     VALRRVDEAGCLVTELALRRPSLDEVFFALTGHPAEPESSAEEPEPEPERSIR"
     gene            6150..6950
                     /locus_tag="VEO00_05305"
     CDS             6150..6950
                     /locus_tag="VEO00_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019984918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU57448.1"
                     /translation="MSVATATIPAGRVGLREAVRHALVLMRRSLLHIKSDPEQLVGMT
                     IQPLMFLVLFVYVFGGAIAGSSREYLQFALPGILVQGLAFTGFSTALGLNKDFQQGLI
                     DRFRSLPISRAAVVSGRIAGDAVRVLWSVVLIVGFGVLIGFRFHGGFAGGIGGLALAA
                     LFGVVMCWPMAFIGIAAKSPEAVNTGGFMVILPLTFASTVFAPAESMPGWLAAFVKVN
                     PITKVVDATRGLLLGGAVARPLWQALVWLVVITAVFAPLAIRLYRRRV"
     gene            complement(6955..8967)
                     /locus_tag="VEO00_05310"
     CDS             complement(6955..8967)
                     /locus_tag="VEO00_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018216712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HYU57449.1"
                     /translation="MAGDTISILDGSTFVVSDRRGDIDAGPDQPHGLFYRDTRFLSRW
                     ILTVDDRPLEVLSTDDVDYFSAQFFLYPPTGTIYTNPALSVIRQRTVGDGIHEDVHML
                     NHSADPVTVRLSVAAHADFADLFEVKDALQKKGDLYREVRDGSLVLGYRREDFVRETV
                     ISSGEPATVGEEGLRFSVEIPPHGDWTTCLDVIPRAEAAPRPKHGHGSIHHPLPNMKL
                     GLDEWIERAPRLESSWDKLGHIYWRSLVDLAALRFYPDILPGASVPAAGLPWFMALFG
                     RDSLITSFQSLPFTPELAQTTLRVLAARQGQTVDDFRDEEPGKILHELRVGELTHFAE
                     RPHSPYYGTADATPLWLVLLEETERWTGDAELVRELEPNARAALAWIDEHGDRDGDGY
                     VEYQRRNEETGLENMCWKDSWNSILHADGSNSTLPRATCELQGYVYDAKRRCARLARE
                     VWDDPALGERLDRDANELKARFNEDFWIPDREFFALALDGEKRQVDSLTSNIGHLLWS
                     GIVDDHRAEAVARHLMSERMFSGWGVRTMAEGMVGYNPIEYHNGTVWPHDSAFVAAGL
                     ARYGYREDAARIAYALLEAATYFDWRLPEVFAGYPRSLTEFPVEYPTASSPQAWATGT
                     PLLLLRVLLGLEPAGDVLRSDPVLPPQIERLSLAGVRGRWADAGAG"
     gene            9284..>9570
                     /locus_tag="VEO00_05315"
     CDS             9284..>9570
                     /locus_tag="VEO00_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYU57450.1"
                     /translation="MKAVVVVPGQAASAHLGDVPDPSLDDVSGGRGVMVRILRVGLDG
                     TDREINAGEYGAAPEGSDFLVLGHECLGVVEEVGPAVTDLAPGDLVVARVRR"
BASE COUNT         1336 a         3343 c         3503 g         1388 t
ORIGIN      
        1 tggaggctcc gcccggtccc gcgggggtgg ggaccgagtt cttcagcacg ggaacggacg
       61 gcaaggtcgc ccggtggtcg gaccggtcgg tggtcacgga ggccacgcgc ccggcggtgt
      121 tcgagttcgt cacggacggc ctgcgccggg ggaagcctgg cagccgcccc tggcgggcca
      181 ccgcggtgca ccgttacgag atcgttccgg cgggcgaggg gtgccgggtg acctacacgg
      241 aggacctgac caggctgacg ggcgcgcccc gcatcctgtt ctccgcaccg atgagccgga
      301 tcgtgttcct gatcgccgcc aggtacatgc gccggggctt cgacaacctg ctggccctgg
      361 ccgaggagcg ggccggcgag cccgcggaag ggagggtgtg atgggatcgc tggcgaggtt
      421 cccggtgtac gcggtgctcc ccgcctcgga cttcgagcgg gccagagcgt ggtaccggga
      481 gaagctggag ctcacgccat cggaggagct gcccggcaac gcctggtacc gctgcgccga
      541 cggaacgtgg ttcatcctca cccagacgcc caacgccaga acggccagaa cacggcggcc
      601 gggttcacgg tgaaggagat cgagtcggcg atggagggac tccgatcgcg gggcgtggtg
      661 ttcgaggagt acgacttcgg cgagatgggc gggaccgaga acggcctgat gaagatgggc
      721 tccacgaagg cggcatggtt caaggacagc gaaggcaaca tcatcgagct cagccagacc
      781 ggctaacctc ggcatccggc cggacccgag gtcagcccgg gtggctacgc ttcgggtgcc
      841 gcgctccgct cgctgtcgag catcgccatc tggcgctcgt cgtagcggtc gccggcggcc
      901 gcgccggggg gcgccgcgcg ctcgatcgcc tcgaggtctg catcggtcag ctgcagctcg
      961 agcgcggcaa gcgcctcggc caggcgcgca cgccgggtcg tccccacgag cgggacgatg
     1021 tcgtcgccgc gggtgaggag ccatccgatc gcgatctggg ccaccgtcgc gcccttctgg
     1081 tcggcgatcg cgcggagggc ctcgacgagc gcgaggttgc gatccaggtt ctccggccgg
     1141 aaccgcggca tgcgcgcccg gatgtcgccg ggcgacagcg cgcgatccct cgaccacgca
     1201 cccgcgagca gcccgcgagc gagcacgccg tacgcggtga cgcccacgcc cagctcgcgg
     1261 caggtcggaa ggatctccgc ctcgatccca cgcgacagca gggagtattc gatctgcaga
     1321 tcggccacgt gatgcaccgc attcgcgcgg cgaaccgtct cggcccccgc ctccgagagg
     1381 ccgacgtggc gcacgtggcc ggccgcgatc atctccgcga tggccccgac cgtgtcctcg
     1441 atcgggaccg ctgggtccac ccgtccgagg cgatagatgt cgacgtggtc ggtgtccagc
     1501 cgccgaaggg tgtaggcgag gaagctcttc acggccaccg gtcgcccgtc cacgccgatg
     1561 aaggcgccgt cgggcgcgcg gagcgctccg aacttgacgc tgatcgcgac ggcgtcgcgg
     1621 tcgcggcctc gcagggcttc gcggatcagg agctcgttgt gacccatgcc gtagtagtcg
     1681 cccgtgtcga gcaggtcgat cccggcgtca agcgccgcat ggatcgtggc gatgctctcc
     1741 gcctcgtcga ccgggccgta ggccccggac atccccatgc agcccaggcc gatcgcggag
     1801 acgcgaggac cggtggtgcc cagccgcagg gtcttcaatg gcatccccct ccttcgagtc
     1861 gacgtgtctt caatgatcgc cggagaccac ggcgaacgcg ccgtagccga cgacgctccc
     1921 acgatcgccg gtcacgtcgc cggaggtgcg gaggtcgagc ggggtgatcg ccaggccgcg
     1981 gtggcgcgcg tgggcgacga ggccgcgcag ggcgtgaacc ccgcaggcgg cgtccgccgc
     2041 gatcgcaaag gcgtcccggg ccaccacagc agccgcggtg cgcccgtcga tcgcctgcgc
     2101 catacggtga tcgcggtagt ggctgaggtc ggtgctgacc aggaccaacg cgcccgcgtc
     2161 ggccagcgcg ccgatcagcg cggcgatctc tccggggtcg gcgtcgccaa cggccaccgg
     2221 gagcacccgg agggccgaag cgagcacccg ctgcaggaac ggtagctgga cctcgagcgc
     2281 atggtcctgg tcatgcggct cttcgtcccg gaccgcgcca caggcggctg ccaccccggc
     2341 gagctccgcg tccacgtcga cggtcccgag cggcgtcgcc caggctcgcg ccgccgatac
     2401 ggcggccccc cgcagcggga cgaagtgcgc cggcccgagc agggccacgc ggtccggccg
     2461 cgaggccgct cgggacagca gcgcgtaggc cgtggcggcg accgggcccg agtagcggta
     2521 gccggcgtgc ggcacgatca gccccagcgg atcggggacg cccgacggcg ggaaggcttc
     2581 ggacagcagg cgatcgacct cggcggccag cgaacgcgga tcgccggggt agaaggtgcc
     2641 ccccgccacc gggcggcgga ccgggggcgt catcctccgc cgagcagcac ggggagccgg
     2701 cgtggccccc accggccggg ctcgctctcg aacacgccgg cgcaccgggt tccgcatgag
     2761 gcgcaccggc cgtcggcggt caggtgccag tccccgagcc ggtaccagtc gcgttcgatc
     2821 agcatccggc cgcagcccgt gcagtacgtg ctctgtccct ccgtgtcatg ggcgtttccc
     2881 gtgtaggcgt ggcggaggcc gttcgccatg gccaggcgcc gggcgcggct cagcgtggcc
     2941 ggcggcgtcg gcggccggtc gcgcatcttc cagtccgggt ggaacgccgt gaagtgcatc
     3001 ggcacgtcgg gcccgaggcg ctcgacggcc caccgcgtca tgtcgtcgat ctcgtcgtcg
     3061 gagtcgttca gcccggggat cagcaggttc gtgacctcca cccagacgtc ggtctcgtgc
     3121 accaggtact cgagcgtctc cttcaccggc tcgaggtggg ccgcgcagac gtcgtggtag
     3181 aagccgtcgg tgaaggcctt caggtcgacg ttcgccgcat ccatgtggcg gtagaagtcg
     3241 gcccgcggat cagcgcacat gtagccggcg gtcaccgcca ccgcccggat gccccgcgcc
     3301 cggcacgcgt cggccacgtc catggcgtac tccatgaaga tcacggggtc gttgtaggtg
     3361 aacgccacgc tgcggcatcc gaggcgctcg gccgccgccg caaggtcctc gggcgaggcg
     3421 gcgtcggcca gcgtgtcgat ctcgcgggac ttggagatgt cccagttctg gcagaaccgg
     3481 caggccaggt tgcacccggc ggtaccgaac gacagcaccg acgtgccggg gaggaagtgg
     3541 ttgagcggct tcttctcgat cggatcgacg cagaacccgc tggaccgacc gtaggtggtc
     3601 aggacgatcc ggtcgtcctg ccgcatccgc acgaagcaca gcccccgctg cccctcgtgg
     3661 agccggcagg cccgcggaca gacgtcgcac tgcacccgcc cgtcgtccag gcggtgccag
     3721 tggcgcgttg ggtacacgtc ggcgggttcg gaaaggcccg tttccacacc tccatgatgc
     3781 gcctaaaacc tgcggaggtc agccagatcc cgcgcacccg aacggtacgc tgttcagcgc
     3841 gatggccccg cgggtcggtg cttcgtcgga accgccaccc tcgcccgagg cgggggcgcc
     3901 ccgctcgacg ccgggcgggg cccggcggta cgtcgcgacc gcggtggcgc tgctcctgac
     3961 cgcggtcgtt gtcgcgacga ccgtgtacct ggtggaccgc cgggagctct cgcgggtccg
     4021 gtcggacctg gcggacgtgc ggcgtgacct cgaggggctg aacgaccgtg tcgccggtct
     4081 caccgacgag cgagaccggt tgcgcgagcg cgtggacgcc ctgtcgcggc tggccggtga
     4141 tccactcgac gacggcgact acaccgttcg ggtgcgcgcc gcggtgcacg gccggccgga
     4201 ccgcctgggc gtctcggtcc tgaccggccc cgcgggcggg acgggctggc ggctgctcga
     4261 ggtggccgcc ggcgccgacg tccggctcgc ggccgtcccc gggctcgacc cgacggtgat
     4321 gaatttcgtg cggttcgcgc ggatgttcaa cggtgggagg cccgaggacg acgccctgca
     4381 taccgtgcgc ttccggatcg ccctggccgg cggccgggtc accgcgatca agagcctcga
     4441 cgcggtggcg ccggccgcgc tcgagccagg cgcgaccggt ccgttcgtcg aggcgctgca
     4501 agagcgactc gccgcgctgc ggttcgacgt gggccccagg gacgggacgt tcggcgacga
     4561 cacctcccac gcggtggtgg ccttccagaa ggtcaacggg ctgcctcgtg acgggacggt
     4621 gacgcgagcg acctgggagg ccatggcgcg accggtgatc ccggccgcac ggaaccggtc
     4681 gagcggggag accatcgagg tcgacctgac ccggcaggtc ctgtacctgg tgcgcgatgg
     4741 gcggatcgtg cgcatcgtcg acgcgtcgac gggcgggggg tacgccttca cgacgaaggg
     4801 cgtccagaag gtcgcgatca cggtcaccgg cgagttccgg atctactgga agatcgaggg
     4861 ctggtaccag agcagcctgg gcccgatgta ccggtcgagc ttctattacc gcggcttcgc
     4921 catccacggg tacggctcgg tgccgccgta ccccgcgagc cacggctgcg tccgcgtgac
     4981 gttcgccgcg gtggaccgcc tgtggccgca cctgttcgag gggatgccgg tctccgtcta
     5041 ccggggctag ggcgccccga gggtcacggt ttccttcgtc cttcctatac tcgaccgaac
     5101 gtcgtcgaca tcgtcggcga cgttctggtt cctcggacgc ccgtgcggaa ggggggaagg
     5161 caggtgaacg aaccggcgat cgtcgccgaa ggcctggtga agcgcttcgg cgacgtgcgg
     5221 gccctcgacg gcgtcgatct ggaggtcgac cagggctcgg tcctcggcct cctcggcccg
     5281 aacggggccg gcaagacgac ggcgatccgg atcctgtcga cgctgctctc tcccgacgcc
     5341 gggagcgccc gggttggcgg cttcgacgtg gtccaccaac cggtccaggt ccgccgcctg
     5401 atcggcctga ccggccagta cgccgccgtg gacgagctgc tgtccgggca cgagaacctc
     5461 tacatgatcg gccggctgtt gggactctcg aagccggccg cgcgccggcg ggcgagcgag
     5521 ctcctggcca acttcgagct gagcgaggcg gccgtgaagg tggtcaaggc gtactccggc
     5581 ggtatgcgcc ggcgcctcga cctggccgcc agcctggtcg ggcggccgcg gttcctctac
     5641 ctggacgagc cgaccaccgg cctcgacccg cgcagccgcc gccagctctg gaacatgatt
     5701 cgcgggctcg tccgcgaggg ggcgaccgtc ctgctgacga cccagtacct ggacgaggcc
     5761 gacagcctgg cgcaccgcat cgcggtgatc gaccacgggc acgtgatcgc cgtggggacc
     5821 tcggacgagc tgaaggcgaa gaccggcggc ctggtgatcg aggcgcggat cgaggcgagg
     5881 tacctcgacg cggtggcgcg gatcctggcc gagatctccg ggaacgaggc ggccatcgac
     5941 cgggaggcga acgtgatcac cgtgccggtc ggcgacccgg gcgcgctgcc ggtcgcactg
     6001 cggcgcgtgg acgaggccgg ctgcctggtg accgagctgg cgctgcgccg gccgagcctc
     6061 gacgaggtct tcttcgccct caccggtcac cccgcggagc cggaatcgtc cgcggaggag
     6121 cccgagccag aacccgagag gagcatccga tgagcgtcgc gaccgccacg atcccggccg
     6181 gccgcgtcgg cctgcgcgag gctgtccgtc acgcgctcgt gctgatgcgc cggagcctgc
     6241 tgcacatcaa gtccgacccg gagcagctgg tcgggatgac catccagccg ctgatgttcc
     6301 tggtgctgtt cgtctacgtg ttcggcgggg cgatcgccgg ctcgagccgg gagtacctgc
     6361 agttcgcgct gccggggatc ctggtgcaag gcctcgcgtt caccggcttc agcaccgcgc
     6421 tcgggctgaa caaggacttc cagcagggac tgatcgaccg gttccgctcg ctcccgatct
     6481 cccgggcggc cgtggtcagc gggcgcatcg ccggggacgc ggtgcgcgtg ctctggagcg
     6541 tggtgctgat cgtcggcttc ggcgtgttga tcgggttccg cttccacggc ggcttcgccg
     6601 gcgggatcgg cggcctggcc ctggcggcgc tgttcggcgt cgtgatgtgc tggccgatgg
     6661 cgttcatcgg catcgcggcg aagagcccgg aggcggtgaa cacgggcggc ttcatggtga
     6721 tcctgccact gacgttcgcc agcacggtgt tcgcgccggc cgagtcgatg cccggctggc
     6781 tcgcggcgtt cgtgaaggtc aatccgatca cgaaggtcgt cgacgccacc cgtgggctgc
     6841 tgctcggcgg ggcggtggcc cggccgctgt ggcaggccct cgtctggctg gtcgtgatca
     6901 ccgcggtgtt cgcgccgctg gcgatccgcc tgtaccgccg ccgggtctga gcggtcagcc
     6961 cgcgccggcg tccgcccagc gcccgcggac gcccgcgagc gacagccgct cgatctgggg
     7021 cgggaggacc ggatcgctgc gcaggacgtc gccggcgggc tccaggccga gcagcacccg
     7081 gagcagcagc aggggggtcc ccgtcgccca ggcctgcggg ctcgacgccg tggggtactc
     7141 gaccgggaac tccgtcagcg agcgcgggta gccggcgaac acttcgggca agcgccagtc
     7201 gaagtacgtc gccgcctcga gcagcgcgta cgcgatgcgg gccgcgtcct cacggtagcc
     7261 gtaccgcgcc agcccggccg cgacgaacgc gctgtcgtgg ggccacacgg tcccgttgtg
     7321 gtactcgatc gggttgtacc cgaccatccc ttcggccatc gtccgcacgc cccagcccga
     7381 gaacatccgc tcgctcatca ggtgccgcgc gaccgcctcg gccctgtggt cgtcgacgat
     7441 cccgctccac agcaggtggc cgatgttgga cgtcaggctg tccacctggc gcttctcccc
     7501 gtcgagggcg agcgcgaaga actcgcggtc ggggatccag aagtcctcgt tgaaccgggc
     7561 cttcagctcg ttcgcgtccc ggtccagccg ctcgccgagc gccggatcgt cccagacctc
     7621 acgggccagc ctggcgcacc ggcgcttggc gtcgtagacg tacccctgca gctcgcacgt
     7681 cgcgcgggga agcgtggagt tgctcccgtc ggcgtgcagg atcgagttcc aggagtcctt
     7741 ccagcacatg ttctccagtc cggtctcctc gttccggcgc tggtactcca cgtagccgtc
     7801 gccgtcgcgg tccccgtgct cgtcgatcca cgccagggcc gcccgggcgt tcggctcgag
     7861 ctcccggacc agctcggcgt cgcccgtcca gcgctcggtc tcctccagca ggacgagcca
     7921 cagcggcgtg gcgtccgcgg tcccgtagta gggggagtgc ggccgctccg cgaagtgggt
     7981 gagctcgccg acgcggagct cgtgcaggat cttgccgggc tcctcgtcgc ggaagtcgtc
     8041 caccgtctgc ccctggcgcg cggccagcac gcgcagcgtg gtctgcgcga gctcgggcgt
     8101 gaacgggagg ctctggaagc tcgtgatgag gctgtcgcgg ccgaacaaag ccatgaacca
     8161 gggaaggccg gccgccggga ccgacgcccc ggggaggatg tccggataga agcggagcgc
     8221 ggcgaggtcc accaggcttc gccagtagat gtgcccgagc ttgtcccagc tcgactccag
     8281 ccggggcgca cgctcgatcc attcgtccaa accgagcttc atgttcggga gcgggtgatg
     8341 gatcgacccg tgcccgtgct tcggacgcgg cgccgcctcc gctcgcggga tcacgtccag
     8401 gcacgtcgtc cagtccccgt ggggtgggat ctcgacggag aagcggagcc cctcctcccc
     8461 caccgtggcc ggctcgcccg acgagatcac ggtctcccga acgaagtcct cgcgccggta
     8521 gccgagcacg agcgacccgt cgcgcacctc gcgatacagg tcccccttct tctgcagcgc
     8581 gtccttcacc tcgaacaggt cggcaaagtc cgcgtgcgcc gccacgctca acctgaccgt
     8641 gaccgggtcg gcggagtggt tcagcatgtg cacgtcctcg tggatgccgt cgccgaccgt
     8701 gcgctgccgg atcaccgaga gcgcggggtt cgtgtagatc gtccccgtcg gcgggtacag
     8761 gaagaactgc gccgagaagt agtccacgtc atccgtcgag aggacctcga gcggccggtc
     8821 atcgacggtc aggatccagc gcgagaggaa gcgggtgtcg cggtagaaca gcccgtgcgg
     8881 ctgatcgggt ccggcgtcga tgtccccccg ccgatcgctc acgacgaagg tgctcccgtc
     8941 caggatgctg atcgtgtccc ccgccatcgg tgctcccttc cggccggccc atccccgcgc
     9001 tctcgccggt cctcgcgctc aggatagggt caagccgatg aaggcagccg tcgaggacgg
     9061 tgaacccccg cgcaacgcag gcggacgact gatccactgc gttcggacgg gacgaagtgg
     9121 tcgcggcgag gaaccggcag tacggatgac cgttggcctc gtgaagatcg accgacggtg
     9181 gaccgttatg cacgagcacc actccgagcc gagcagctga gctccccgac caggcgctcc
     9241 cttccgagcg ccgcgtcctc gggctcgggt tagggtgagg cggatgaagg ccgtggtcgt
     9301 cgtcccgggg caggcagcga gcgcccacct cggagacgtc ccggacccgt cgctcgacga
     9361 cgtctccgga ggccggggcg tcatggtgcg gatcctgcgg gtgggactgg acggaaccga
     9421 ccgcgagatc aacgccggcg agtacggggc ggcccccgag gggtcggact tcctggtgct
     9481 gggacacgag tgcctgggcg tcgtcgagga ggtcggtccc gcggtgacgg acctcgcccc
     9541 gggggacctg gtggtcgccc gcgtgcggcg
//
LOCUS       DASPWO010000236         4870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_205551, whole genome shotgun sequence.
ACCESSION   DASPWO010000236 DASPWO010000000
VERSION     DASPWO010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205551"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VEO00_05320"
     CDS             complement(<1..218)
                     /locus_tag="VEO00_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HYU57451.1"
                     /translation="MSTPPATGLGTGVGDARVIVVVGPGGVGKTTTAAAIAVRTAGEG
                     RRTIVLTVDPARRLASALGLPDIPGDPVP"
     gene            complement(215..1168)
                     /locus_tag="VEO00_05325"
     CDS             complement(215..1168)
                     /locus_tag="VEO00_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HYU57452.1"
                     /translation="MDLAGARVAIVSGKGGTGKTTVACALALGAARAGRSVLLVELEG
                     RQGAARLLGLRPATFEERPTPLGFSLESVGPRDALLEYLRLFYGITRVAGPLLRSRAV
                     ELATEAAPGFRDMMLAGKLYEVAEWRRGSPRGRGRPRYDLVVADAPPTGQIVPFLSAP
                     RVFAELIRMGRPHGQARRIDAFLRRRALILLVTTAEELAVTETLEARDAIGALGMRVG
                     PVIVNRLLEDPLPRGTVRAFERLAPADARRLAQAAGAPMPQPAAATALEVARTQRARA
                     REASAQVGRLGDSPVIRLPFLFSERFGPAEVARLAATLEEA"
     gene            1196..2509
                     /locus_tag="VEO00_05330"
     CDS             1196..2509
                     /locus_tag="VEO00_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HYU57453.1"
                     /translation="MRRLPLLALLVASVLWPLATPVRAQASNQVEVVKVEGIIDRTVE
                     GFLLSRLEDVPDGGALLLQIDSPGTLGVDAVALARRFAEAPVPVVAWAGPPGALVQGG
                     AVLLFEAAAIAATSGGSGIGPARPIDLGRRPDAETGDAVGRAAAAAAGFAEARDRDGE
                     AARRLVEDAATILAPQAALDAGVADLAAASAPTLLAEIDGQLVETALGPVELVTQGDP
                     GSFTLRFSDLGPVARVLHAVASPTASYVLLVLALWAVAFEVTQPGFGLAGVAAVVLGG
                     LAIYSLTVAPAGALGLALLLSGVAALTLDVRLRRVGLLTLLGVGAFAAGSLLVFRRVA
                     ASVDPSVWLIGFAVVGTALYFGFALTVATKAREEAITARQTGLVGLVGEAASDLDPEG
                     HVLVKGAMWQARAVAAPIAGGARVRVRGVDGLVLQVTEESRPDQG"
     gene            2600..2869
                     /locus_tag="VEO00_05335"
     CDS             2600..2869
                     /locus_tag="VEO00_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HYU57454.1"
                     /translation="MARGDLGWQFRAACRGDASGLFFPPHTSEERDERRERESRAKAI
                     CSGCAVRAECLEYALRIREPHGIWGGLNELERRHLLRARDREGAA"
     gene            2916..3383
                     /locus_tag="VEO00_05340"
     CDS             2916..3383
                     /locus_tag="VEO00_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYU57455.1"
                     /translation="MGVEERLADLGLELPVPAAPVAAYVPVVLTGSLAFVSGQVPLEE
                     GTPLVRGHVGAEVSVEDGRAAARRCALQALAALKAKLGSLDRVRRIVKLSVFVASAPG
                     FTEQPAVANGASELLTEVFGESGRHARTAVAVPDLPLGAPVEVDVIAEVGPPH"
     gene            complement(3380..>4870)
                     /locus_tag="VEO00_05345"
     CDS             complement(3380..>4870)
                     /locus_tag="VEO00_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HYU57456.1"
                     /translation="DLVDDATVQRRREEIRARLAGTAMADASIVPCSAATGAGVADVA
                     AALDALVAAAPAPETDGRPRLFVDRVFTIRGAGTVVTGTLTGGPLRVDDRVELYPSGT
                     QARVRGLQRHERPLQEAWPVSRVAVNLGGLTTRDALRGDVLGMPGQWRPTRVLDAEVR
                     PVRGLSHPLTARGSHKLHAGAAERDARLRLLEVDAIRPGERALVRVTLGRQLALDVGE
                     PFVLREAGRRETVAGGIVLDPDPRGSPADPERLRRRAAAARSELPGLLVAERGAIRAT
                     EVGPLTGAFGDAAGAERAGPWLVAPEVLEALAAAAHGLLAEHHAARPLLPGLELDAVR
                     RALAERVPAVAPALEQSLAGALVERLAADGRLARDGTNVRLPGHRVSLGEREPEARRL
                     EEALRSGWPTPPTARELGVDRELAAALVATGRVVAAGDDVLLLPERWAEAVRVARELA
                     ARPEGMTAGAFRVALGTSRKYAIPLLERLDAEGVTRRDGDVRRAVG"
BASE COUNT          537 a         1845 c         1901 g          587 t
ORIGIN      
        1 gggaccgggt cgccggggat gtcgggaagc ccgagcgccg aggccagccg ccgggccggg
       61 tccacggtca ggacgatcgt ccgccggcct tcgccggccg tgcgcaccgc gatcgccgcc
      121 gcggtggtgg tcttgccgac cccgccgggc ccgaccacga cgatcacccg ggcatcgccg
      181 accccggtcc ccagcccggt ggccggcggg gtgctcacgc ctcctccagc gtcgccgcca
      241 gccgcgccac ctcggcaggc ccgaaccgct cggagaacag gaacgggagc cggatcaccg
      301 gggagtcacc cagcctccca acctgcgccg aggcctcccg ggcccgggcc cgctgcgtcc
      361 gggccacctc gagcgcggtc gcggccgccg gctgcggcat cggggcgccg gccgcctggg
      421 ccagccgccg ggcgtcggcg ggcgccaggc gctcgaacgc gcggacggtc ccacggggca
      481 ggggatcctc cagcagccgg ttcacgatca ccgggcctac gcgcatcccg agcgcgccga
      541 tcgcgtcacg cgcttcgagg gtctccgtga cggcgagctc ctcggccgtg gtcaccaaga
      601 ggatcagggc ccgccggcgc aggaacgcgt cgatccgcct cgcctggccg tgcgggcgtc
      661 ccatccggat cagctcggcg aagacgcggg gcgccgacag gaacgggacg atctgcccgg
      721 tcggcggggc atcggcgacc accaggtcgt agcgcgggcg gccgcgaccg cgcgggctgc
      781 cgcgccgcca ttcggcgact tcgtagagct tcccggcgag catcatgtcc cggaaacccg
      841 gcgcggcttc ggtggcgagc tcaacggccc gcgagcggag cagcgggccg gcgacgcggg
      901 tgatgccgta gaacaggcgc aggtactcga ggagggcgtc ccgcggcccg accgattcca
      961 gcgagaaccc gagcggggtc ggccgttcct cgaacgtcgc cggccgcagg ccgagcaggc
     1021 gtgccgcgcc ctgccggccc tccagctcga cgagcagcac ggagcggccg gcgcgtgccg
     1081 cgccgagcgc gagcgcgcag gccacggtgg tcttccccgt gccgcccttg cccgagacga
     1141 tcgccacgcg cgcgccggcc aggtccacgc cgccggatgc tagtgtgcgg cgccgatgcg
     1201 gcgcctcccc ctcctcgctc tgctcgtcgc gtccgtcctg tggccgctcg ccacgccggt
     1261 ccgggcgcag gccagcaacc aggtcgaggt ggtgaaggtg gaggggatca tcgaccggac
     1321 cgtcgagggc ttcctgctgt cgcggctgga ggatgtcccc gacggcggcg cgctcctcct
     1381 gcagatcgac tccccgggaa cgctcggcgt ggacgccgtc gcgctggccc ggcggttcgc
     1441 cgaggccccg gtcccggtcg tggcctgggc cggcccgccc ggggcactcg tccagggggg
     1501 cgccgtcctt ctcttcgagg cggcggcgat cgcggcaacc tcggggggct cggggatcgg
     1561 gccggcgcgg ccgatcgacc tcgggcgccg ccccgacgcc gagacggggg acgcggtggg
     1621 gcgcgccgcc gcagcggcgg ccgggttcgc ggaggcgcgc gaccgcgacg gggaggcggc
     1681 ccggcgcctg gtcgaggatg cggcgacgat cctcgcgccg caggccgccc tcgatgccgg
     1741 cgtcgccgac ctggccgccg ccagcgcgcc cacgctcctg gccgagatcg acgggcagct
     1801 cgtcgagacg gcgctcggcc ccgtcgagct cgtgacgcag ggcgaccccg ggagcttcac
     1861 cctgcggttc agcgatctcg gtcccgtcgc ccgcgtcctg cacgcggtcg cctcgccgac
     1921 ggcgtcctac gtgctgctgg tgctcgcgct gtgggcggtg gcgttcgagg tcacccagcc
     1981 gggcttcggc ctggccgggg tggccgcggt ggtgctcggg gggctggcga tctactcgct
     2041 gacggtggcg ccagcgggcg cgctcggcct ggcgctgctg ctctccggcg tcgcggccct
     2101 gaccctcgac gtcaggctcc gccgggtggg actgctgacg ctcctcggcg tcggcgcgtt
     2161 cgccgccggg tcgctcctcg tgttccggcg cgtcgctgcg tcggtcgacc cctcggtgtg
     2221 gctgatcggg ttcgccgtgg tcggcaccgc gctctacttc gggttcgcgc tgaccgtcgc
     2281 gacgaaggcc cgggaggagg cgatcaccgc gcggcaaacc ggcctggtcg ggctggtggg
     2341 cgaagcggcg agcgacctgg accccgaggg ccacgtcttg gtcaagggcg cgatgtggca
     2401 ggcgcgcgcc gtggcggccc cgatcgccgg gggcgcgcgg gtgcgggtgc gcggcgtgga
     2461 cgggctggtc ctgcaggtca ccgaggagtc ccgccccgac cagggctagg ccccagcaca
     2521 cggaaccggt ccaatccctc gaacgtctca tggcgaagaa ctcccgaagg aggcccgggg
     2581 gttgggaggg agcacgtcga tggctcgggg ggacctcgga tggcagttcc gggcggcgtg
     2641 ccgcggggac gcttccgggc tgttcttccc gccccacacg tccgaggagc gggacgagcg
     2701 ccgcgagcgc gagtcacggg ccaaggccat ctgttcgggg tgcgccgtcc gcgccgagtg
     2761 cctcgagtac gcgttgcgca tccgcgagcc ccacggcatc tggggcgggc tgaacgagct
     2821 ggagcgccgc cacctcctgc gcgcgcgcga ccgggagggc gctgcctgac gtccgtcgct
     2881 tgtccgggag cccgcccggc tggcaggatg gcctcgtggg cgtggaggag cgtctggccg
     2941 atctggggct cgagcttccg gtgcccgccg ccccggtcgc cgcgtacgtc cccgtcgtcc
     3001 tgaccggctc gctcgcgttc gtgtcgggcc aggttcccct cgaggagggc acgccgctcg
     3061 tgcgcggcca cgtcggggcg gaggtctccg tcgaggacgg acgggccgcg gcccggcgct
     3121 gcgccctgca ggcgctggcg gcgctgaagg cgaagctcgg ttccctcgac cgggtgcgcc
     3181 ggatcgtgaa gctgtcggtc ttcgtggcgt cggcgccggg gttcacggag cagcccgcgg
     3241 tcgcgaacgg agccagcgag ctgctgaccg aggtgttcgg ggagagcggg cggcacgccc
     3301 gcaccgcggt cgccgtcccc gatctcccgc tcggcgctcc ggtcgaggtc gatgtgatcg
     3361 cggaggtcgg cccgccccac tagcccacgg cacggcgcac gtcaccgtcg cggcgggtga
     3421 cgccctcggc atcgagccgt tcgaggagcg ggatcgcgta cttccggctg gtcccgagcg
     3481 ccacccggaa cgcgccggcg gtcatcccct caggccgtgc cgccagttcg cgggccaccc
     3541 ggacggcctc tgcccaccgc tcggggagga gaaggacgtc gtcgccggcc gccaccaccc
     3601 ggccggtggc caccagggcg gcggccagct cgcggtccac cccgagctcg cgggcggtcg
     3661 gaggggtggg ccacccgctc cgcagtgcct cctccagtcg gcgcgcctcc ggctcccgct
     3721 cgccgagcga gacccggtga ccgggaagac ggacgttcgt cccgtcgcgc gccagtcgcc
     3781 cgtcggcggc aagtcgctcc acgagcgcgc cggcgaggga ttgctccagc gccggcgcca
     3841 ccgcggggac gcgctcggcg agcgcgcggc gcacggcgtc gagctcgagc ccgggcagga
     3901 gcggccgggc ggcgtggtgc tccgcgagca ggccgtgggc cgcggcggcg agggcttcca
     3961 ggacctccgg ggcgaccagc caggggccgg cgcgctcggc gccggcggca tcgccgaacg
     4021 ctccggtcag cgggccgacc tcggtcgccc ggatcgcccc ccgttccgcg acgagcaggc
     4081 cgggaagctc cgagcgggcg gcggccgccc ggcggcggag gcgctccggg tcggcggggc
     4141 tgccgcgcgg atccgggtcg aggacgatcc cgccggccac cgtctcccgc cggccggcct
     4201 cccgcagcac gaacggttcg ccgacgtcga gcgccagttg ccggccgagg gtgaccctga
     4261 ccagcgcgcg ttcgccgggg cggatcgcgt cgacctcgag gagccgcagg cgggcgtcgc
     4321 gctccgccgc gccggcatgg agcttgtgcg atccccgcgc ggtcagaggg tggctcagtc
     4381 cgcggaccgg gcggacctcc gcgtcgagga cgcgcgtcgg gcgccactgg ccgggcattc
     4441 cgagcacatc accgcgcagg gcgtcgcggg tggtcagccc gccgaggttc accgccaccc
     4501 gcgatacggg ccacgcctcc tgcagcggac gttcgtggcg ctgcaacccc cgaacgcggg
     4561 cttgcgtgcc ggacgggtag agctcgaccc ggtcgtcgac gcgcagcggc ccgccggtca
     4621 acgtcccggt caccacggtc ccggcgccgc ggatcgtgaa gacgcggtcg acgaacagcc
     4681 gcgggcggcc gtcggtctcg ggggccggcg cggccgccac gagcgcgtcg agcgccgccg
     4741 ccacgtcggc cacgcccgcc ccggtggccg ccgagcacgg gacgatcgag gcgtcggcca
     4801 tggccgtacc ggccaggcgc gcgcggatct cctcgcggcg ccgttgcacg gtcgcgtcgt
     4861 ccaccaggtc
//
LOCUS       DASPWO010000237         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_803047, whole genome shotgun sequence.
ACCESSION   DASPWO010000237 DASPWO010000000
VERSION     DASPWO010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803047"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VEO00_05350"
     CDS             complement(<1..790)
                     /locus_tag="VEO00_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU57457.1"
                     /translation="MRFGLFYEHQLPRPWEDGAEERLLSDALEQVELADRLGLHSVWE
                     VEHHFLEEYSHSSAPEVFLAAASQRTERIRLGHGIVQLPPAVNHPARVAERIATLDLV
                     SRGRVEFGTGEGSSQVELGGFGVVRDEKRDQWDEALDAVTRMFVEEPFAGYRGRWIDA
                     PQRNVVPKPKQKPHPPLWVACSRRETILLAARKGLGALSFSFIEPEAAKEWSDEYYRL
                     LSSDECVPAGFSVNPNLAVVLPFMCHQDEATAIARGIDGAHFFGY"
     gene            complement(889..1290)
                     /locus_tag="VEO00_05355"
     CDS             complement(889..1290)
                     /locus_tag="VEO00_05355"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HYU57458.1"
                     /translation="MTGWRTALLYDSDCAFCRWATNKLLTWDRHGRLEPVALQDPRGD
                     ELLAGMSEDQKMGSWHLVTADGRRYSAGAAVPPLMRLLPGGRPIAAMAETFPRTTERL
                     YRWVSRHRGQMANLLGAKACSVDPKPREDAA"
     gene            1386..>2050
                     /locus_tag="VEO00_05360"
     CDS             1386..>2050
                     /locus_tag="VEO00_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYU57459.1"
                     /translation="MSRSEELFERALRVIPGGVNSPVRAFRAVGGTPRFIARGEGAYV
                     EDVDGHRYVDFVQSWGALLFGHARTEIVDAAVTAAERGTSFGAPTEAEVLLAERIAGM
                     VPSIEQVRLVSSGTEACMSAIRLARGATGRSKVVKFEGCYHGHADGLLAKAGSGVATF
                     GLPGSAGVTGGAAGDTIILAYNDREAVRDVLRAIGDEVAAVILEPVAANMGVVLPEAG
                     FLEG"
BASE COUNT          284 a          713 c          744 g          309 t
ORIGIN      
        1 agtagccgaa gaagtgcgcc ccgtcgatcc cgcgggcgat cgcggtcgcc tcgtcctggt
       61 ggcacatgaa cggcaggacg acggccaggt tcgggttgac cgagaagccc gccggcacgc
      121 attcgtccga ggacagcagg cggtagtact cgtcggacca ttccttcgct gcctcgggct
      181 cgatgaagga gaaggagagc gcgcccaggc ccttccgcgc ggccaggagg atcgtctccc
      241 ggcggctgca cgcgacccac agcggtgggt gaggtttctg cttcggcttc ggcaccacgt
      301 tgcgctgcgg cgcgtcgatc cagcgtcccc ggtacccggc gaagggctcc tcgacgaaca
      361 tccgggtgac ggcgtcgagc gcctcgtccc actggtcgcg tttctcgtcg cggaccacgc
      421 cgaacccgcc gagctcgacc tgcgagctgc cctcgccggt gccgaactcg acccggccgc
      481 ggctcaccag gtccagggtc gcgatccgct ccgcgacccg ggcggggtgg ttgaccgccg
      541 gagggagctg gacgatcccg tgcccgagcc tgatgcgctc ggtgcgctgg ctggccgcgg
      601 ccaggaacac ctcgggcgcg ctcgaatggg agtactcctc caggaagtgg tgctcgacct
      661 cccacacgct gtggaggccc agccggtcgg ccagctcgac ctgctcgagg gcgtcggaga
      721 ggagccgttc ctccgctccg tcctcccacg ggcgggggag ctggtgctcg tagaacagtc
      781 cgaacctcat gtcgatgctc cttcggctga tgacgtccgt ccgtgggcca cgccaggccc
      841 ggcggccgag caatgctcgt tcaccgcggc ggatcggcgc aagcggcgtc aggccgcgtc
      901 ctcgcgcggc ttggggtcga cggagcaggc cttcgccccc agcaggtttg ccatctggcc
      961 tcggtggcgc gagacccacc gatagaggcg ctcggtggtt cgggggaacg tctcggccat
     1021 cgcggcgatg ggtcgccctc caggaagcag ccgcatgagc ggcgggacgg ccgcacccgc
     1081 cgagtaccgc cggccgtcgg cggtcaccag gtgccatgac cccatcttct ggtcctcgct
     1141 catgccggcc agcagctcat cgcctcgggg gtcctggagc gccaccggct ccagccttcc
     1201 gtgtcgatcc caggtcagga gcttgttcgt cgcccaccgg cagaaggcgc agtccgagtc
     1261 gtagagcagc gcagtgcgcc agccggtcat accaccagcc tacggccacc ccggcgaagg
     1321 cgcctcggat gccgtcactg cgagcgaacc gttaccgtgg gcgctccagt gtggagcgat
     1381 cttcgatgag ccggtccgaa gaactcttcg agcgggctct acgcgtgatc ccgggcgggg
     1441 tgaactcgcc ggtgcgggcg ttccgggcgg tcgggggcac ccctcggttc atcgcgcgag
     1501 gggagggcgc ctacgtcgag gacgtcgacg gccatcggta cgtcgacttc gtccagtcgt
     1561 ggggagccct gctgttcggc catgcccgca ccgagatcgt cgatgcggcc gtgaccgcgg
     1621 ccgagcgggg cacgtcgttc ggggctccca ccgaggccga ggtcctgctg gcggagcgga
     1681 tcgcggggat ggtcccaagc atcgagcagg tccgcctggt ctcctccggc accgaggcct
     1741 gcatgagcgc gatccgactg gcgcgcggag ccaccgggcg gtcgaaggtc gtgaagttcg
     1801 agggctgcta ccacggccac gccgacgggc tcctggcgaa ggcgggatcg ggcgtcgcca
     1861 cgttcggcct gcccggatcg gccggcgtga ccgggggggc cgccggggac accatcatcc
     1921 tcgcctacaa cgaccgcgaa gcggtgcggg acgtgttgcg cgccatcggt gacgaggtcg
     1981 ccgcggtcat cctcgagccg gtggcggcga acatgggggt ggtcctcccc gaggccggct
     2041 tcctggaggg
//
LOCUS       DASPWO010000238         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_35382, whole genome shotgun sequence.
ACCESSION   DASPWO010000238 DASPWO010000000
VERSION     DASPWO010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35382"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VEO00_05365"
     CDS             complement(<1..827)
                     /locus_tag="VEO00_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57460.1"
                     /translation="MQEDLLRDSVRRYFRPFSPFYRRLLDEAGVGPRDVRRLSDLQRI
                     PMSSASSFSGQTDPARPFEALFRPDEQTLKRFLHRSLMRRVVVEKFLRGDEAAERVLA
                     EEFKPVHLQLPFAGGPLVGFSMRDLVAMAQAGARMLAVLGLARTDVLVSTLPFDAGLP
                     SWFATYAAQAAGMSAAHLGGGQAVRPSQTASWMARLGATAFVTQPGYAEGLLRAAPAS
                     AFAALRVLALWGGVGMLGARARFAELLRASGAPAAQAAMVLSVPETRVAWGECPAPAG
                     "
     gene            980..1387
                     /locus_tag="VEO00_05370"
     CDS             980..1387
                     /locus_tag="VEO00_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57461.1"
                     /translation="MRAAAAVLLLLSLSLAGCDDLTSPGPSAPASTTPGSPAATGEGQ
                     PPVVQIGGTIASIDEASLTVAQPDGAEVRMRRLAGDATRFLFPDGDGYRQLAPAEVAG
                     IAPGTRACVEALLDGQVVLAIRVFLDSVCGAQR"
     gene            complement(1388..>2111)
                     /locus_tag="VEO00_05375"
     CDS             complement(1388..>2111)
                     /locus_tag="VEO00_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HYU57462.1"
                     /translation="MSHTEFGGRAINGFDAVDGGQANDCNGHGTHVAGTVGGTTFGVA
                     KAVTIVAVRVLNCDGVGTTVDVVAGVDWVTGNHAANQPAVANMSLGGSPSPALDQAVK
                     GSIQDGITYAVAAGNGNIFGQAVNACNVSPARVPAAITVSATNKSDAKPTWANKGKCV
                     DWFAPGVDVKSAWASSNTATAVLSGTSMSTPHTTGVAALFLQTHPAAQPGTVRQALFA
                     RTTKDIVTSSSTPNNDLLFTNF"
BASE COUNT          281 a          779 c          767 g          284 t
ORIGIN      
        1 ccggcaggcg ccgggcactc gccccacgcc acgcgcgtct ccgggaccga caggaccatc
       61 gcggcctggg cggccggcgc gccgctcgcc cgcagcagct cggcgaaccg cgcccgcgcg
      121 ccgagcatcc cgacacctcc ccacaacgcc aggacgcgca gcgcggcgaa ggcgctcgcc
      181 ggcgccgctc gcagcaagcc ctccgcgtac ccgggctggg tcacgaacgc ggtggccccc
      241 agacgggcca tccacgacgc ggtctgggag gggcggaccg cctggccgcc cccgaggtgc
      301 gcagccgaca tgccggcggc ctgcgccgca tacgtcgcga accaggacgg cagtccggcg
      361 tcgaacggca gcgtcgagac caggacgtcg gtgcgggcca gcccgagcac tgcgagcatc
      421 cgcgcgccgg cctgggccat cgccaccagg tcgcgcatgc tgaacccgac cagcggcccc
      481 ccagcgaacg gcagctgcag gtggacgggc ttgaactcct cggccaggac ccgctcggcc
      541 gcctcgtccc cgcggaggaa cttctcgacc accacccgtc gcatcagcga ccggtgcagg
      601 aagcgcttca gggtctgttc gtccggccgg aacagcgcct cgaaggggcg cgccgggtcg
      661 gtctgcccgg agaacgacga cgccgaggac atcggaatcc gctggaggtc cgagagtcgc
      721 cgcacgtcgc gcggcccgac cccggcctcg tccagcagcc ggcgatagaa ggggctgaac
      781 gggcgaaagt accgccgcac ggagtcccgg aggaggtcct cctgcatgcg gaccacgtcc
      841 ttgggcgaga gccggtccca ggcgcttgac agggtgaccc cctagccgag caacgagagg
      901 gcgggccgga gcccgccctc tcggatggta gccgccccct tccgggcggt tcaaggaccg
      961 cgggtggttc aatccggtcg tgcgcgcggc cgcggcggtg ctcctgctcc tttccctctc
     1021 ccttgccggg tgcgacgacc tgacgtcccc cggcccgtcg gcccccgcct cgacgacgcc
     1081 cgggtcgccg gccgcgacgg gcgaggggca accccccgtg gtgcagatcg gcggcacgat
     1141 cgcgtcgatc gacgaggcgt cgctgacggt ggcgcagccc gacggcgcgg aggttcggat
     1201 gcgccgcctg gcgggggacg ccacgcggtt cctgttcccc gacggcgacg gctaccggca
     1261 actggcgccg gccgaggtcg ccgggatcgc gccggggacg cgggcctgcg tcgaggcgct
     1321 gctcgacggc caggtcgtgc tggcgatcag ggtgttcctc gactccgtct gcggcgcgca
     1381 gcggtgatca gaagttggtg aagaggaggt cgttgttcgg tgtcgacgag ctcgtgacga
     1441 tgtccttggt cgtccgggcg aagagcgcct ggcggacggt gccgggctgg gcggccgggt
     1501 gggtctgcag gaacagggcc gccacgccgg tcgtgtgcgg ggtggacatc gacgttcccg
     1561 agaggaccgc ggtggccgtg ttgctcgatg cccaggccga cttgacgtcc acgccgggcg
     1621 cgaaccagtc cacgcacttc cccttgttgg cccaggtcgg cttggcgtcg ctcttgttcg
     1681 tcgcgctgac cgtgatcgcc gcggggaccc gcgccggcga gacgttacac gcgttcaccg
     1741 cctggccgaa gatgtttccg ttccccgccg cgaccgcgta ggtgatgccg tcctggatcg
     1801 agcccttcac cgcctggtcg agcgccgggc tcggcgaccc cccgagactc atgttggcga
     1861 cggcaggctg gttcgcggcg tggttccccg tcacccagtc gaccccggcg acaacgtcga
     1921 ccgtggtgcc gacgccgtcg cagttcagca cgcgtaccgc gacgatcgtc acggccttcg
     1981 cgacgccgaa cgtggtcccg ccgacggtgc cggcgacgtg ggtcccgtgg ccgttgcagt
     2041 cgttggcctg gccgccgtcc accgcgtcga acccgttgat ggcccgtccg ccgaactcgg
     2101 tgtgcgacat g
//
LOCUS       DASPWO010000239         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_376476, whole genome shotgun sequence.
ACCESSION   DASPWO010000239 DASPWO010000000
VERSION     DASPWO010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376476"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VEO00_05380"
     CDS             <1..1245
                     /locus_tag="VEO00_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HYU57463.1"
                     /translation="PLNPKAMIMGIRDMAVEAEHGLNLIPNSPLVRETYGLDDGLAPE
                     RLAKPLVDDAIPYDAVWDCVTCGACVEACPVLIEHVDKIVGLRRNLVLEESRFPSELT
                     GAFRNMEQVGNPWGQPPATRLAWAKDLPFAVPSLAEVAAEGRLDDLEVIYWVGCAAAF
                     DERNRRVARAFAACLDAAGVSFAVLGQEESCTGDPARRMGNEYVFQILAQGNVQTLER
                     YRAGERTIVTACPHCFNTLANEYGQFGGQYRVVHHSQYLADLLESGRLRTLAADGESR
                     TVTYHDACYLSRYNGVAASPRDVLGAVPGVELREMERSGRNTFCCGAGGGRMWMEETR
                     GTRINVERTRQALETGADTVATGCPFCMTMLKDGLADAGRGAGTDAPVASADIAEILA
                     GSLAASPWAGARGGDRELPVIN"
     gene            1667..>2591
                     /locus_tag="VEO00_05385"
     CDS             1667..>2591
                     /locus_tag="VEO00_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU57464.1"
                     /translation="MSLSTAATGYRLTEEQLMLRDAVRILADERIAPRAAEIDRTAEF
                     PTDVKDLLASHDILGLPFPSEYGGVGGDLLSVCLAVEAIARVCATSALILAVQELDSL
                     PIMLAGSAEQKERWFPDLAAGRRLIAFALTEAEAGSDAAAIRTRATRDGAEYVISGSK
                     RFISQGNVADLVVVFAVTDPDAGRHRNLSCFVVEAGTPGFSAPRLEHKMGIRGSPTAE
                     LAFDEVRVPEANRLGEAGDGFSIAMRTLDRSRPGVAAQAVGIAQGALEVAARYATERR
                     QFGRPIGDFQLIQGMLADMDAATEAARQLLYK"
BASE COUNT          388 a          930 c          903 g          370 t
ORIGIN      
        1 ccgctcaacc cgaaggccat gatcatgggc atccgggaca tggccgtcga agccgagcat
       61 ggcctgaacc tcattcccaa ttcgccgctg gtccgcgaga cgtatggcct ggacgacggg
      121 ctcgcaccgg agcggctcgc gaagccgctc gtggacgacg ccatcccgta cgacgccgtc
      181 tgggattgcg ttacgtgcgg agcctgcgtg gaggcctgtc cggtgctcat cgagcacgtg
      241 gacaagatcg tcggtcttcg ccggaatctc gtcctcgaag agagccgctt cccctcggag
      301 ctgaccggcg cgttccggaa catggagcaa gtgggcaacc cctggggcca gccgccggcc
      361 acccgcctgg cctgggcgaa ggacctcccg ttcgcagtgc cgtccctcgc ggaggtcgcg
      421 gcggagggcc ggctcgacga cctggaggtg atctactggg tcggctgtgc cgccgccttc
      481 gacgaacgca acaggcgcgt ggctcgggcc ttcgccgcgt gtctcgacgc cgccggcgtc
      541 tcattcgccg tgctcggcca ggaagagtcc tgcacgggcg acccggcgcg ccggatgggc
      601 aacgagtacg tcttccagat cctcgcccag ggcaacgtac agacgctgga acgctaccgg
      661 gcgggcgagc gaacgatcgt caccgcctgc ccccactgct tcaacacgct ggccaacgag
      721 tacggccagt tcggcggtca ataccgcgtc gtccaccaca gccagtacct ggccgacctc
      781 ctggagagcg gccggctccg aaccctggcg gccgacggcg agtcgcggac cgtcacctac
      841 cacgacgcgt gctacctgtc ccgctacaac ggcgttgccg cgtcaccccg tgacgtcctg
      901 ggcgccgttc ccggcgtcga gctccgcgag atggagcgaa gcggccgcaa cacgttctgc
      961 tgcggtgcgg gcggcggccg catgtggatg gaggagacgc gcggcacgcg aatcaacgtg
     1021 gagcgcaccc gccaggccct ggagacgggc gccgacaccg tcgcgacggg ttgcccgttc
     1081 tgcatgacca tgctcaagga cgggctggcc gatgcgggac gcggtgccgg caccgatgca
     1141 ccggtcgcgt cggccgacat cgccgagatc ctcgccggca gcctggccgc aagcccttgg
     1201 gcaggcgcgc gcggtggcga ccgggagcta ccggtcatca actgaccgct cgcccccgtc
     1261 gcataggctg atcggccgtc gccgtcgttg acgaccgacc tcccgctacg aactcagcct
     1321 ggcaggcgat gcccgcagtg gaggcaggtg ctgtccgtcc agaggttcgg ccggccgcag
     1381 ctcgggcaga ccttcatgcc ttcggtggac gtggcgaagg ggctctcacg ggtggccgcc
     1441 tgtcgccgat cgcgaaggag gatccagatc gccgcggcca gcccgacggc tgcggcagca
     1501 agcatcgcga gtatccacgc ttggtccatg gcgcgccccg caggctcgga tgggcggacg
     1561 cgcccccgga gcacgaccgg ccatcgcggc aaatctacgc ctcggacggt cactgcgcca
     1621 gacagcgggc ccggaacgcg gcgaggccgg tgctacgatg ccggtcgtga gcctgtcaac
     1681 ggccgccacg ggctaccgcc tcaccgaaga gcagctcatg ctccgggacg cggtccgcat
     1741 cctggccgat gagcgcatcg cgccgcgcgc ggcggagatc gatcgaacgg ccgagttccc
     1801 gacagacgtc aaggatctcc tcgccagcca cgacatcctg gggctgccct ttccctccga
     1861 gtacggcggc gtcggtggcg acctgctgtc ggtctgcctg gcggtcgagg ccatcgcgcg
     1921 ggtatgcgcg acgtccgccc tcatcctggc cgtccaggag ctggactctc tgccgatcat
     1981 gctggccggc tccgccgagc agaaggagcg ctggttcccg gacctggccg ccggccgccg
     2041 gctcatcgcc ttcgcgctca cggaggccga ggcgggatcg gacgcggccg ccatccggac
     2101 ccgggccacc cgcgatggcg ccgagtacgt catcagcggc agcaagcggt tcatcagcca
     2161 gggcaacgtc gccgatctcg tcgtcgtgtt cgcggtcacc gatccggatg ctggccggca
     2221 tcgcaacctg agctgcttcg tggtggaggc gggcacgccc ggcttcagcg cgccgcgcct
     2281 cgagcacaag atgggcatcc ggggctcgcc gaccgccgag ctggccttcg atgaggtacg
     2341 ggtcccggag gccaaccgcc tgggtgaggc gggcgacggc ttctcgatcg ccatgcgcac
     2401 gctcgatcgc tcgcggccgg gagtggccgc ccaggccgtg gggatcgccc agggcgcact
     2461 ggaggtcgcc gcccgctacg ccaccgagcg ccgccagttc ggtcgcccga tcggcgactt
     2521 ccagctcatc cagggcatgc tggccgacat ggacgccgcg acggaggcgg ctcggcagct
     2581 gctgtacaag g
//
LOCUS       DASPWO010000240         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_457434, whole genome shotgun sequence.
ACCESSION   DASPWO010000240 DASPWO010000000
VERSION     DASPWO010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457434"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VEO00_05390"
     CDS             complement(<1..797)
                     /locus_tag="VEO00_05390"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HYU57465.1"
                     /translation="MHIEDERLYPLLERYLAGETSAGDAEIVREWLAEDAAHPQLLAD
                     LRLIKHVTSDTPPPTSVDAAWMQAVKTLGLRRKPRVSRRSLMVALAAAAVLIAVIGGG
                     RVLRRAPQWKEYATAAAQRMVIRLQDGTQVTIAPKSRVRYTADFGRAHRDLYLDGEAY
                     FQVAPDSQRPLRVHTSGSVTEDLGTAFVVNAYADQIATEVVVTEGRVSLSRGETTSSV
                     VLGLRDLGRLDASGRATVRRGVDVDRYLAWTKGVLAFDGEPLRDVVLT"
     gene            complement(910..>2574)
                     /locus_tag="VEO00_05395"
     CDS             complement(910..>2574)
                     /locus_tag="VEO00_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HYU57466.1"
                     /translation="EFFLSGRRLSWWLLGVSLVATTFSTDTPNLVTDLVRTGGVSQNW
                     VWWAFAITGMCTVFFYAKLWRRSAALTDIAFYESRYSGKPAAFLRGFRAIYLGVFFNV
                     MIMASVNLSATKIAGVMLGWDRSTAVLVGGAVTVLYAASSGLSGVVLTDFVQFVIAMV
                     GSVMAAVVALKLPQVGGLSGLFSHPAVTGHLSLLPDFSDWKTAAAVLVVPLAVQWWST
                     WYPGAEPGGGGYVAQRMLAAPTERDAMRSTLLFNVLHYAVRPWPWIIVALASLIIYPD
                     LVSIAARFPSIDKAIVRHDLAYSAMLVYLPHGLLGLMVASLAAAYMSTISTHLNWGAS
                     YAVEDCYRRFVAPDKTERHYVMVGRAVTVGLIVLASAVSLWLHNSYQAFQILLQIGAG
                     TGLIFLLRWFWWRINAWSEIAGMAISFVVAVYFQFVHTALGFAPLAPTVQLVIGIAIT
                     TVGWLAVTFLTPPTDRATLQTFYDRIRPMGPGWKAAVTTGPANPGESVSAAFLCWFLG
                     VAVIYAALFGTGYVLYGKQLPGIACLVVAGLAAWGLFRTLPRVGFE"
BASE COUNT          498 a          864 c          856 g          356 t
ORIGIN      
        1 gtgagtacga cgtcacgcaa tggctcgccg tcgaaggcca gcacaccctt cgtccacgcg
       61 aggtatcggt cgacatcgac gccacgtcgc accgttgcgc ggccgctggc atcgagccgc
      121 ccgaggtccc ggagcccgag caccacggac gaagtcgtct cgccccgcga gagcgacacc
      181 cgcccctcag tcacgacgac ttcggtcgcg atctgatcgg cgtacgcatt gaccacgaac
      241 gccgtcccca gatcctcggt gacgctgccg gatgtgtgga cgcggagtgg acgctgggag
      301 tcgggtgcga cttggaaata ggcttcgccg tccagataca ggtcgcggtg cgctcggccg
      361 aagtcggccg tgtagcgcac gcgactcttg ggcgcgatgg tgacctgcgt gccgtcttgc
      421 agccgaatga ccatgcgctg cgcagcggcg gtcgcatatt ccttccactg cggcgctcgc
      481 cgcagcacgc gccctccccc gatcaccgcg atgagaaccg ccgcagcggc gagcgccacc
      541 atgagcgacc gccgcgacac ccgtggcttc cgtcgaaggc cgagggtttt gaccgcctgc
      601 atccaggccg catcgacgct ggtcggtggt ggcgtgtcgc ttgtgacgtg cttgatcagc
      661 cgcagatccg cgaggagttg cgggtgcgcg gcgtcttccg cgagccactc gcgcacgatc
      721 tccgcgtctc ccgccgacgt ctccccggcc aggtagcgct cgagcagggg atagagacgc
      781 tcgtcttcga tatgcatact gctatgacgg ggcaaccacg tcccaacccc caagagggct
      841 cggtgctagg ggctaggggc taggggctcg ggttggcagg cctaacccct agccccgaac
      901 ccctagcccc tactcaaatc cgactcgagg cagggtgcgg aacagccccc aggccgccaa
      961 gcccgcgacg actaagcacg cgatcccggg cagctgcttc ccgtacagca cgtatcccgt
     1021 gccaaacagc gcggcgtaga tgacggcgac acccaggaac cagcacagga aagccgcgga
     1081 cacgctttcg ccggggtttg ccggcccggt ggtgaccgcg gccttccatc ccgggcccat
     1141 gggccgaatc cggtcataaa acgtctggag cgtagcgcga tcggtgggcg gtgtcaggaa
     1201 cgtcaccgcc agccacccga cagtggtgat cgcgatgccg atcacgagct ggacggtcgg
     1261 ggcgagcggc gcgaacccga gcgccgtatg cacgaactgg aagtagacag ccaccacgaa
     1321 cgagatcgcc atccccgcga tttcgctcca cgcgttgatc cgccaccaga accagcgcag
     1381 caggaagatc agtccggtcc ccgcgccgat ctggagcagg atctggaacg cctggtagga
     1441 gttgtgcaac cagagcgaga cggccgacgc gagcacgatc agtccgaccg tgacggcgcg
     1501 cccgaccatc acgtagtggc gttcggtctt gtcgggtgcc acgaagcggc ggtagcagtc
     1561 ctcgaccgcg taggaagcgc cccagttcag atgggtgctg atggtggaca tgtacgccgc
     1621 cgcaagtgag gcgaccatga ggccgaggag cccgtgtggg agatagacga gcatcgcgga
     1681 gtaggccaga tcgtgccgta cgatcgcctt gtcgatggag gggaaccgcg cggcgatgga
     1741 aacaaggtcg ggatagatga tgagcgacgc gagggccacg atgatccacg gccacggccg
     1801 cacggcgtag tgaagcacgt tgaacagcag cgtcgagcgc atggcgtcgc gctccgtggg
     1861 tgcggcgagc atacgttgcg ccacgtagcc cccacctccc ggctcggcgc cgggatacca
     1921 ggtgctccac cactgcactg cgagcggcac gacgaggacc gcggccgccg tcttccagtc
     1981 cgagaaatcg ggtaagagcg acaggtgccc cgtcaccgcg gggtgggaga acagccccga
     2041 caggccgccg acctggggga gcttgagcgc cacgacggcc gccatcacgg acccgaccat
     2101 cgcgatcacg aactggacga aatcggtcaa gacgacaccc gacaacccgg agcttgccgc
     2161 gtacagcacg gtgacggcgc cgccgacgag caccgccgtc gagcgatccc agccgagcat
     2221 caccccggcg atcttggtcg cggaaaggtt gaccgaggcc atgatcatca cgttgaagaa
     2281 cacgccgagg tagatcgccc gaaacccacg gaggaaggcc gcgggcttgc cggagtagcg
     2341 cgactcgtag aacgcgatgt cggtgagggc cgccgaacgc cgccacagct tggcgtagaa
     2401 gaacacggtg cacattcccg tgatcgcaaa ggcccaccag acccagttct ggctcacgcc
     2461 gccggtgcgt acgagatcgg tgacgaggtt cggcgtgtcg gtcgagaacg tcgtcgccac
     2521 gagcgacacg cccagcagcc accacgacag ccggcgcccc gagagaaaga actc
//
LOCUS       DASPWO010000241         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_457859, whole genome shotgun sequence.
ACCESSION   DASPWO010000241 DASPWO010000000
VERSION     DASPWO010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457859"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VEO00_05400"
     CDS             complement(<1..439)
                     /locus_tag="VEO00_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HYU57467.1"
                     /translation="MNPAARGEPALTEDQLLAAIRKVLSGQGPGVLAGVGDDAALVET
                     GSGDGVLTTDLLVEGVHFDRGTTGPRDLGYKAIVVNVSDLAAMAASPRYALVSIALPP
                     GVGPSWVMELYGGMRTACEEYALSLVGGDLSRGGEVVISVSAMG"
     gene            681..878
                     /locus_tag="VEO00_05405"
     CDS             681..878
                     /locus_tag="VEO00_05405"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HYU57468.1"
                     /translation="MNTIELEGVSDKSWGDAAREALREAAKTIRGINRLEVLSTSTRV
                     DGERIVEFRTQVRLYFLVESR"
     gene            975..2492
                     /locus_tag="VEO00_05410"
     CDS             975..2492
                     /locus_tag="VEO00_05410"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HYU57469.1"
                     /translation="MSVEAKQVHDNPSPDELRAFTEEMPSCRIAEFDNVNVQTRVTSR
                     SAGSTYIVTDDPSTASGQSMTREEYARIATLQDDYIREREMVVVDGYIGNDADFRVPA
                     RLSIEKANANIAGMQQKLYFPREGEEPRLHVIYTPNLPAPGYPDERCIAVDLEAGVTR
                     VLNSDYFGESKKGGLRMWNTIVYDRGGLSLHAGCKVVPANGGEKVFLIIGLSGTGKTT
                     TTFTTQNDSLPVQDDFVALMPGGKVYGSENGCFAKTFSLDPHFEPNIFNAVTKPDSYL
                     ENVYQDAQGKVNFFETSYTKNGRAVFGMDALGRFRDAREVGAVDFLLILNWNENIIPA
                     VARLSQAQAAAYFMLGETTGTSAGGKAEEGKFLRVPGTNPFFPLPHGLQGNRFLDLLA
                     THPIEVFLMNTGRAGGKADDKRSKKVKIPHSSAIVQAIAENTIKWTDSPDFGYEVAED
                     VPGIDDVELLQPWRLYEAQDRMDEYRQIVERLTAERRDRLAEFPELGQDIVRAVG"
     gene            complement(2557..2847)
                     /locus_tag="VEO00_05415"
     CDS             complement(2557..2847)
                     /locus_tag="VEO00_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57470.1"
                     /translation="MGVFELSVAGILALGGVLSFGKWVRRPFASPRLGDQVLYALHRT
                     GRVGLWFAMAGLFAGYALLEEPQRFRWYVYVPIALAIMQLLAGIALGRSRSE"
     gene            complement(2867..>3246)
                     /locus_tag="VEO00_05420"
     CDS             complement(2867..>3246)
                     /locus_tag="VEO00_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HYU57471.1"
                     /translation="AAQEDEIARRQEVFRKGRAASDVSGRTVVVVDDGVATGGTAVAA
                     CRWARAAGARKVVLAVPVAPRQAIPHLSEEADELVVLATPDPFYAVGQWYQDFEQVSD
                     EEVARILDRAAQDRDAGSGPGGP"
BASE COUNT          548 a         1161 c         1134 g          403 t
ORIGIN      
        1 cccccatcgc cgacaccgag atgaccacct cgccgccgcg ggacaggtcg ccgcccacga
       61 gcgagagggc gtactcctcg caggccgtcc gcatcccccc gtacagctcc atcacccacg
      121 agggccccac gcccgggggc agcgcgatgg acacgagcgc gtagcggggg ctcgccgcca
      181 tcgcggcgag gtcggacacg ttcacgacga tggctttgta gcccaggtcg cgcgggccgg
      241 tggttccccg atcgaagtgc acgccctcca ccagcaggtc ggtggtgagc acgccgtccc
      301 ccgagccggt ctccacgagc gccgcgtcgt cccccacccc ggccaggacg cccgggccct
      361 ggcccgacag gaccttccgg atggcggcga ggagctggtc ctcggtcagg gcgggctcgc
      421 cgcgtgccgc gggattcacg agcgctccct cccgtgggca cggccagcac cgagggcgtc
      481 gatgtgcccc gagcgcaagg ccccgaggag cgccgcgcac cgtgcggtac gcaagcgacg
      541 aggaacgccg cgatcgggga tgcagcggcg tccgaattcg gccgcggccg cgggagggag
      601 cgccatatac tcagcgtacc cgggcggttg tccgctgggg ttcttccgaa caggagagag
      661 gaggcctcgc cgtggcggtc atgaacacca tcgagctcga gggcgtgtcc gacaagagct
      721 ggggcgacgc cgctcgagag gcgctgcgcg aggcggccaa gacgatccgc ggaatcaacc
      781 ggctggaggt cctctccacc tcgacacggg tggacggcga gcgcatcgtg gagttccgca
      841 cgcaggtccg cctgtacttc ctcgtcgagt caaggtagcc gccggcggcg gcacgtccgg
      901 ttcgggccac aggccggttg ggtagaatcg gctccccgcc agtggcgtgg tccctccacg
      961 aaacaggagc cgacgtgtcc gtcgaagcga agcaggtcca cgacaaccca tcgcccgacg
     1021 agctcagggc gttcaccgaa gagatgccga gctgccgcat cgccgagttc gacaacgtga
     1081 acgtgcagac gcgggtgacc tcccgcagcg ccggcagcac gtacatcgtc acggacgacc
     1141 cctcgacggc cagcggccag agcatgaccc gcgaggagta cgcgcgcatc gccacgctcc
     1201 aggacgacta catccgcgag cgggagatgg tggtggtcga cggctacatc gggaacgacg
     1261 cggacttccg ggtcccggcg cgcctgtcca tcgagaaggc gaacgcgaac atcgccggca
     1321 tgcagcagaa gctgtacttc ccccgggagg gcgaggagcc gcggctgcac gtcatctaca
     1381 cgccgaacct cccggcgccg gggtacccgg acgagcgctg catcgcggtc gacctggaag
     1441 ccggcgtgac gcgggtgctc aactccgact acttcggcga gtcgaagaag ggcggcctgc
     1501 ggatgtggaa caccatcgtc tacgaccgtg ggggcctgtc gctgcacgcg ggatgcaagg
     1561 tcgtgccggc gaacggcggc gagaaggtgt tcctcatcat cgggctctcc ggcacgggca
     1621 agacgaccac cacgttcacc acgcagaacg actcgctgcc ggtgcaggac gacttcgtgg
     1681 ccctgatgcc gggcgggaag gtctacggct ccgagaacgg ttgcttcgcg aagacgttct
     1741 cgctcgaccc ccacttcgag ccgaacatct tcaacgcggt cacgaagccg gactcctact
     1801 tggagaacgt gtaccaggac gcgcagggga aggtgaactt cttcgagacc tcgtacacga
     1861 agaacggccg ggcggtgttc ggcatggacg cgctggggcg gttccgcgac gcgcgcgagg
     1921 tcggcgcggt cgacttcctg ctcatcctga actggaacga gaacatcatc ccggcggtgg
     1981 cgcggctctc gcaggcgcag gccgcggcgt acttcatgct cggcgagacc accgggacgt
     2041 cggcgggcgg caaggccgag gaggggaagt tcctgcgggt gccggggacg aaccccttct
     2101 tccccctgcc gcacggcctc caggggaacc gcttcctgga cctgctggcc acgcacccca
     2161 tcgaggtgtt cctgatgaac acgggacggg cgggcggcaa ggccgacgac aagcggtcga
     2221 agaaggtgaa gatcccccac tcgtccgcca tcgtgcaggc catcgccgag aacaccatca
     2281 agtggacgga ctcgccggac ttcggctacg aggtggccga ggacgtcccg ggcatcgacg
     2341 acgtcgagct gctccagccg tggcgtctgt acgaggccca ggaccggatg gacgagtacc
     2401 gccagatcgt ggagcgcctc accgccgagc gccgggaccg cctcgcggag ttccccgagc
     2461 tcggccagga catcgtccgc gccgtcggct gaccggaccc ggcacgagcg aacgcgcgag
     2521 acccgatccc ggcacgaccg ccacgcgggc catccgctac tcggagcggg agcggccgag
     2581 ggcgatgccc gccagcagct gcatgatggc cagggcgatc gggacgtaga cgtaccaccg
     2641 gaaccgctgc ggctcctcca gcagcgcata gccggcgaag agcccggcca tggcgaacca
     2701 cagccccacc cggcccgtgc ggtgcagcgc gtacagcacc tggtcgccca ggcgcggcga
     2761 ggcgaacgga cgtcggaccc acttcccgaa cgagagcacc ccgccgagcg cgaggatccc
     2821 ggccaccgac agctcgaaga cgcccacggg tcagggcctc acggggtcag gggccgccgg
     2881 gcccggaccc ggcgtcgcgg tcctgggcgg cccggtcgag gatccgggca acctcctcgt
     2941 cgctcacctg ctcgaagtcc tggtaccact gtcccaccgc gtagaagggg tcgggcgtgg
     3001 ccaggaccac cagctcgtcg gcctcctccg acaggtgcgg gatggcctgg cgcggcgcga
     3061 cggggaccgc caggacgacc ttcctggcgc cggccgcccg cgcccaccgg cacgccgcca
     3121 cggccgtccc cccggtggcc acgccgtcgt cgaccacgac gacggtccgg cccgagacgt
     3181 ccgaggccgc ccggcccttc cggaacacct cctggcggcg ggcgatctcg tcttcctggg
     3241 cggcga
//
LOCUS       DASPWO010000242         5111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_36098, whole genome shotgun sequence.
ACCESSION   DASPWO010000242 DASPWO010000000
VERSION     DASPWO010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36098"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VEO00_05425"
     CDS             complement(<1..1119)
                     /locus_tag="VEO00_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HYU57472.1"
                     /translation="MSGEVAIVGMACVFPGARDVQAYWENIIHKVDAVSDPPPDWEGQ
                     LFLDPGVEANDRLYCARGGYLGRLAEFSPAEYGVMPNSVDGAEPDHFLALRAAFDAIA
                     DAGYLGRDIDRERTEVIIGRGTYVNRGNTTVIQHGIVVDSVLRILRQLHPEHGDEELA
                     AIKRQLKASLPAFNAETAPGLVPNIISGRIANRLDCMGPNFIVDAACASSLVAVDLGM
                     RDLLSGRCDLAVVGGVHASTPPPIVMIFCQLKAISKRGAIRPFDARADGTLLGEGVGM
                     VVLKRREDAERDGDRVYAIVKGVGVASDGKALGLLAPRLEGEELALRRAYEAAGVSPS
                     TVGLIEAHGTATAVGDVVEIDALNRVFGRRGGDAPTCAL"
     gene            complement(1116..>5111)
                     /locus_tag="VEO00_05430"
     CDS             complement(1116..>5111)
                     /locus_tag="VEO00_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYU57473.1"
                     /translation="KVALALHHKVLPPTLHVETPNPRLREADNPFFVSTDALPWLPAE
                     PGTPRRAGVSSFGFGGTNFHAVLEEHVDDYTDPMARKTWEAWPAELFVWNGASAAGLE
                     AALAALSTPLERGARPVLRSLAAAVCSAADGGSGNDSRRLAIVATDLDDLRGKLGVAR
                     EALKTGRSRLQDPKGVYLASGDRPRGRLAFLFPGQGSQSPHMMRDLALHFPHLRVACE
                     QADRVLRGRLPRRLSGYMHPPSAFSPAEQSAQMQAITDTVVAQPALGAVEVGLLHLLR
                     DLGLEPDMVAGHSYGEYVALAAAGVLSEDALFEISEARGRAIKESVGETPGTMAAVLA
                     PAETVAAALRDLPNVWLANFNAPKQTIIAGAPADLERAMAVLASAGLRAKPIPVACAF
                     HSPLMERARVRLAAELARLELKPPRIPVFSNTEAGAYPSEPARMAALLSEHLVRPVKF
                     LDEIRAMYDRGARLFVEVGPKSVLTSLVRQILDRPDAAAIPLDVSDRNGLVQLLHALA
                     QVTASGLNVRLGRLFAGRSVGKLELDKLDESRAPSPAWVVSGAGARPAGQAPGRPAPT
                     LPPVPIPAEPPAVQEAVSAPAAPADAVVLQFQSLMSQFLQTQAAVMSAYLRGGGAADE
                     AALEMRAPVALSASPLPVISAPAATLAVVAASSPAEAPAAPRPAAAARLPSAGPRDVA
                     GELLRIVSERTGYPADMLNPDLNLEADLGIDSIKRVEILSAFQRRQSAEDQGRVSGVM
                     ERLTSRKTLREAIATLDEALSGASGPVPPAAAGGPPPERTPAVAPALPDVEEVPRLTL
                     RAVGAPLEKARSRSFSGRAWLITDDEGGLAEAIAARLVQLDERPVILRQEAAVSAQGP
                     ARYGADLTRPEQVAAVLDRVRGAHGAPGVILHLLPLKAGPGFDGLSIDAWRERVQSDI
                     KTLYGLLRAAGDDLTAAGRAGGAMVVAATGLGSSLAGGEAAVSPTHGGVAALLKTVAA
                     EMPEVGCRAVHLDPASPAEEKAAQLLAEVGSRDGPVEVGYSGGRRVTVVPRLAPLGEG
                     AHGSNGAEVGPDWVFLITGGARGITAEIASYLASVFKPTLVLAGLSPRPEDEAPETAA
                     IDDPQQLKAALTASMRAANAAVRPSEVEVAWQRLLRSREIRRNLDGLRQTGARVEYQP
                     VDVRDEAAFGGLIDGIYRTHGRIDAVIHGAGIIEDKLLRDKTAESFDRVVHTKTDSAF
                     ILARRLRPEGLKLLIFMSSVTATFGNRGQADYGAANGVLNALATLLSFRWPARVRALN
                     WGPWDKTGMVSEQVKRQFASRGIQVISASAGVAAIAREIKARDSEPIVVVGGGPWVAD
                     AVRPPELEVTA"
BASE COUNT          703 a         1877 c         1878 g          653 t
ORIGIN      
        1 cagcgcgcag gtcggcgcgt cgcccccgcg gcggccgaac acccggttga gggcgtcgat
       61 ctccaccaca tcgcccaccg cggtggcggt gccgtgcgct tcgatgaggc cgacggtgga
      121 cggggagacg ccggccgcct cgtaggcgcg gcgcagggcc agctcttcgc cttcgagccg
      181 gggcgcgagc agccccaggg ccttgccgtc gctcgccact ccgacgccct tcacgatcgc
      241 gtagacccgg tccccgtcgc gctcggcgtc ctcccggcgc ttgagcacca ccatgccgac
      301 cccctcgccg agcagggttc cgtcggcgcg ggcgtcgaag ggccggatcg ccccccgctt
      361 cgagatggcc ttgagctggc agaagatcat cacgatgggc gggggggtcg aggcgtgcac
      421 gccgcccacc accgcgagat cgcagcggcc gctcaagagg tctcgcatcc cgaggtccac
      481 cgccaccagg gacgacgcgc aggccgcgtc gacgatgaag ttggggccca tgcagtcgag
      541 gcggttggcg atgcggcccg agatgatgtt cggcaccaga cccggcgcgg tctccgcgtt
      601 gaaggcgggc aggcttgcct tgagctgccg tttgatcgcg gccagctcct cgtcgccgtg
      661 ctccggatgg agctgcctga ggatgcggag gaccgagtcg accacgatcc cgtgctggat
      721 caccgtggtg ttgccgcggt tcacgtaggt gccgcggccg atgatcacct cggtgcgctc
      781 gcggtcgatg tcccgcccga ggtagccggc gtcggcgatg gcgtcgaagg ccgcgcgcag
      841 ggcgagaaag tggtcgggct cggcgccgtc caccgaattc ggcatgacgc cgtactcggc
      901 ggggctgaac tccgccagac gccccaggta cccgccgcgc gcgcagtaga gccggtcgtt
      961 ggcctcgacc ccgggatcga ggaagagctg cccctcccag tcgggaggcg ggtcggagac
     1021 cgcgtccacc ttgtggatga tgttctccca gtaggcctgg acgtcccgcg cgcccgggaa
     1081 cacgcacgcc atcccgacga tggcgacctc gccgctcacg ccgtgacctc cagctccggg
     1141 gggcgcacgg catccgcgac ccagggaccg ccgccgacca cgacgatcgg ctccgagtcc
     1201 cgggccttga tctcgcgggc gatcgccgcg accccggccg aggccgagat cacctggatg
     1261 ccccggctcg cgaactgccg cttcacctgc tccgagacca tgcccgtctt gtcccacggc
     1321 ccccagttca gggcgcgcac gcgggccggc cagcggaacg acagcagggt ggcgagggcg
     1381 ttcagcacgc cgttggcggc gccgtagtcg gcctggcccc ggttgccgaa ggtggccgtg
     1441 accgacgaca tgaagatcag gagcttcagg ccctcgggcc gcagccgccg ggcgaggatg
     1501 aatgcgctgt ccgtcttggt gtggaccacg cggtcgaagg actccgcggt cttgtcccga
     1561 aggagcttgt cctcgatgat gccggccccg tggatgacgg cgtcgatgcg gccgtgcgtc
     1621 cggtagatcc cgtcgatcag ccctccgaag gcggcctcgt cgcgcacgtc gaccggctgg
     1681 tactccaccc gggcgccggt ctgtcggagg ccgtccaggt tgcggcggat ctcccggctg
     1741 cgcagcagcc gctgccaggc cacttccacc tccgagggcc tcacggccgc gttcgccgcg
     1801 cgcatggacg cggtgagcgc cgccttgagc tgctgcggat cgtcgatagc cgcggtctcg
     1861 ggcgcctcgt cctccggacg cggggagagt cccgccagga ccagcgtcgg cttgaacacc
     1921 gaggcgaggt aggacgcgat ctcggcggtg atgccgcgag cgccgcccgt gatgaggaag
     1981 acccagtccg gtcccacctc cgcgccattc gagccgtggg cgccctcccc cagcggtgcg
     2041 aggcggggaa cgacggtgac tcggcgcccg ccggagtacc cgacctcgac cgggccgtcc
     2101 cgcgagccga cctcggccag gagctgggcc gccttctcct ccgccggcga ggccggatcg
     2161 agatgcaccg cgcggcagcc gacctccggc atctccgcgg cgacggtctt cagcagggcg
     2221 gccacgccac catgggtggg agagacggcc gcctcgccgc ccgccagaga ggagccgagg
     2281 cccgtcgcgg cgaccaccat cgcccccccc gcacggccgg ccgcggtgag atcgtccccg
     2341 gcggcgcgaa ggagcccata gagggtcttg atgtcggact gcacgcgctc gcgccaggca
     2401 tcgatcgaaa gaccgtcgaa gccgggcccc gccttcaggg gcagcaggtg caggatgacc
     2461 cccggcgcgc cgtgcgcacc gcggacgcgg tcgagcaccg ccgcgacctg ctcgggccgc
     2521 gtcaagtcgg cgccgtaacg ggcgggcccc tgcgcggaga ccgccgcctc ctgacgcagg
     2581 atcacgggcc gctcgtccag ttgcacgagc cgggcggcga tcgcctcggc caggccgccc
     2641 tcatcgtcgg tgatgagcca cgcccggccc gagaagctcc gcgagcgcgc cttctcgagc
     2701 ggcgcgccca cggccctcaa ggtgagacgg ggcacctcct ccacgtcggg cagcgcgggc
     2761 gcgaccgccg gcgtcctctc cggaggtggc ccgccggccg cggccggcgg gacggggccg
     2821 gacgcgcccg acagcgcctc gtccagggtc gcgatcgcct cgcgaagcgt cttgcggctc
     2881 gtgagacgct ccatcacccc cgagacgcgg ccctgatcct cggcggactg cctccgctgg
     2941 aacgcgctca ggatctccac ccgcttgatg gagtcgatgc ccaggtcggc ctcgaggttc
     3001 aggtcgggat tgagcatgtc cgccggatag ccggtccgct cgctgacgat gcggagcagc
     3061 tcaccggcga catcgcgcgg cccggccgat ggcagacgag ccgccgccgc cggccgtggc
     3121 gcggcgggcg cctcggccgg agacgaggcc gccaccacgg cgagcgtggc ggccggcgcg
     3181 gaaatgacgg gcagagggga cgcggagagg gccacgggtg cccgcatctc gagcgccgcc
     3241 tcgtcggcgg cgccgccgcc tcgcaggtac gcgctcatca ccgcggcctg tgtttgcagg
     3301 aactgcgaca tcaggctctg gaactgcagt acgaccgcat cggcgggcgc ggccggcgcc
     3361 gagaccgcct cctgaacggc gggcggctcc gccgggatcg ggaccggcgg cagggtgggc
     3421 gcgggacggc ccggcgcctg tcccgccggc cgggcccccg cgccactcac gacccacgcc
     3481 gggctcggag cgcgggactc gtcgagcttg tcgagctcga gcttgcccac ggagcgtccc
     3541 gcgaacaggc ggccgagcct cacgttgagc ccggaggccg tgacctgggc caaagcgtgg
     3601 agcagctgca ccaacccgtt gcggtccgac acgtcgaggg ggatggcggc ggcgtcgggc
     3661 cggtcaagga tctggcggac gaggctcgtg agcacgctct tcggcccgac ctcgacgaag
     3721 aggcgggccc cgcggtcgta catggcccgt atctcgtcga ggaacttgac cggcctcacc
     3781 aggtgctcgg agaggagcgc ggccatccgc gccggctcgg acgggtacgc ccccgcctcg
     3841 gtgttcgaga acaccggaat gcgcggcggc ttcagctcca ggcgtgccag ctcggccgcc
     3901 agccggaccc gcgcgcgctc catgagcggg gaatggaagg cgcaggccac cgggatcggc
     3961 ttcgcccgga gcccggccga cgcgagcacg gccatggcgc gctccaggtc cgcgggagca
     4021 ccggcgatga tcgtctgctt gggggcgttg aagttggcca gccatacgtt cggcaggtcg
     4081 cggagcgcgg cggcgaccgt ctccgccggg gcgagcacgg ccgccatcgt gcccggggtc
     4141 tcccccaccg actccttgat cgcgcggccc ctcgcctccg agatctcgaa gagggcatcc
     4201 tccgagagga cgccggccgc ggcgagggcc acgtactcgc cgtagctgtg gccggccacc
     4261 atgtccggtt ccagccccag gtcgcgcaag agatggagca gccccacctc gaccgcgccc
     4321 agcgcaggtt gggcgaccac ggtgtcggtg atcgcctgca tctgcgcgct ctgctcggcg
     4381 gggctgaagg cggagggcgg gtgcatgtag ccactcaacc gccggggcaa gcgtccgcgg
     4441 agcacgcggt ccgcctgctc gcaggcgacg cgcaggtgcg ggaaatggag cgcgaggtcc
     4501 cgcatcatgt gcggggactg cgagccctgg ccggggaaca ggaaggccag cctcccccgg
     4561 ggccgatccc cactggcgag gtagacgccc ttcgggtcct ggaggcggct ccggcccgtc
     4621 ttgagcgcct cccgggccac cccgagcttg ccccgcaggt cgtcgaggtc ggtggccacg
     4681 atcgcgagcc gccgcgagtc gttgcccgac ccgccgtccg ccgcggagca gaccgccgcc
     4741 gccagggagc gcagaacggg gcgggcgccg cgctccaggg gcgtcgagag cgccgccagc
     4801 gccgcctcga ggccggcggc ggacgcgccg ttccacacga acagctcggc cggccaggcc
     4861 tcccacgtct tgcgcgccat cggatcggtg tagtcgtcga catgctcttc gagaacggcg
     4921 tggaagttgg tcccgccgaa gccgaacgag ctgacgcccg cccggcgcgg cgtacccggc
     4981 tcggcgggca gccacggcag cgcatcggtg ctcacgaaga acgggttgtc ggcctcgcgc
     5041 agcctggggt tcggggtctc gacgtgaagg gtcgggggca gcaccttgtg gtggagcgcg
     5101 agggccacct t
//
LOCUS       DASPWO010000243         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_36204, whole genome shotgun sequence.
ACCESSION   DASPWO010000243 DASPWO010000000
VERSION     DASPWO010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36204"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /gene="nuoG"
                     /locus_tag="VEO00_05435"
     CDS             complement(<1..1282)
                     /gene="nuoG"
                     /locus_tag="VEO00_05435"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HYU57474.1"
                     /translation="MSGGEALATLTIDGKELTVPKGTLIIRAAEELGIEIPRFCDHPL
                     LEPVAACRQCYVKVEGQRKLMTSCSTPVADGMVVFTQYADEEVRTAQTAVLEFLLINH
                     PLDCPVCDRGGECPLQDQALAFGPGESRYLEAKRTYRKPLPLSPLVNLDRERCVLCAR
                     CTRFCDQISGDRFIELFDRGAAEQVSIAPGEDFRSPFSGNTIQICPVGALTSSVYRFA
                     ARPFDLQTGDSICPHCACGCNLRVDMRRGQVVRHLARDNADVNDAWTCDKGRFAHQFA
                     DTPTRLETPLLRLRGLEPVAFDEALTAVASWCADRKVAILAGGRLSDEDAYALSKLAR
                     TVLHTNDVDHRMSGAPDIPLQVERAQAAGTPVTYRDVERAKVIVLVGLDAEQELPILH
                     LRIRKAVHGHEAKVFVVHPRRTRLWDVGEHLLCLP"
     gene            complement(1279..>2030)
                     /locus_tag="VEO00_05440"
     CDS             complement(1279..>2030)
                     /locus_tag="VEO00_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HYU57475.1"
                     /translation="NVETLSCVPHIIDRGAEWFGSIGTERSTGPGVFSISGKVNRPGN
                     YEAPMGTTARELIEEYAGGILEGKRIKAWTPGGSSTPFLTGDHLDVQMGFDEVQAAGS
                     LLGTKAMIVLDETDCVVDAVLRFTQFYAHESCGKCTPCREGTWWMTRVLRRMQMGRGR
                     MEDIDLMRDVGQNMLFKSFCALADGAVSPIDSSIKYFRDEYEEHVRLGRCPFQESPRL
                     EAASARTGGTRGVLAQVLPEGLEIELGEVLG"
BASE COUNT          312 a          713 c          688 g          317 t
ORIGIN      
        1 ccggcaggca gagcaggtgc tcccccacgt cccacaaacg ggtccggcgg ggatggacca
       61 cgaacacctt cgcctcgtgg ccgtggaccg cctttcggat ccgcaggtgc aggatgggta
      121 gctcctgttc ggcgtccagc cccaccagca cgatcacctt ggcccgctcg acgtcccggt
      181 aggtcaccgg ggttccggcc gcctgagccc gttcgacctg cagcgggatg tcgggggcgc
      241 cgctcatccg gtggtcgacg tcgttcgtgt ggagcaccgt gcgggccagc ttcgacaggg
      301 cgtaggcgtc ttcgtccgac aggcgccccc cggccaagat ggccaccttg cggtccgcgc
      361 accacgacgc cacggccgtc agcgcttcgt cgaacgccac cggctccaac ccccgcaggc
      421 gaagcagcgg ggtctccagg cgggtcggcg tgtccgcgaa ctgatgggcg aaccggccct
      481 tgtcgcaggt ccaggcgtcg ttgacgtcgg cgttgtcccg ggccaggtga cggaccacct
      541 ggccccgacg catgtcgacc cgcaggttgc agccgcacgc gcagtgcggg cagatcgaat
      601 cgccggtttg caggtcgaac ggccgggcgg cgaaccggta caccgacgag gtcagcgccc
      661 ccaccgggca gatctggatg gtgttgccgg agaacggcga gcggaagtcc tcaccgggcg
      721 cgatggacac ctgctcggcg gcgccccggt cgaacagttc gatgaaccgg tcgccggaga
      781 tctggtcgca gaaccgggtg caccgggcgc acagcacgca gcgctcgcgg tccaggttga
      841 ccagcgggga cagcgggagc ggcttacggt aggtgcgctt ggcctcgaga tatcggctct
      901 cgcccggccc gaaggcgagt gcctggtcct gcagcggaca ctcgccgccc cgatcgcaca
      961 ccgggcagtc gagcgggtgg ttgatcagca ggaactcgag cacggcggtc tgggcggtcc
     1021 ggacctcctc gtcggcgtac tgggtgaaga cgaccatgcc gtcggcgacg ggcgtcgagc
     1081 aggacgtcat gagcttgcgc tgtccctcga ccttgacgta gcactggcgg cacgcggcca
     1141 ccggctccag cagcggatgg tcgcagaacc gggggatctc gatgcccagc tcctccgcgg
     1201 cgcggatgat cagcgttccc ttgggaacgg tgagctcttt cccgtcgatg gtcagcgtgg
     1261 cgagggcctc gccgccgctc aacccaggac ctccccgagc tcgatctcga gaccttccgg
     1321 aagcacctgg gccagcacgc cccgggtccc gccggtccgc gccgatgcgg cttccagccg
     1381 ggggctctcc tggaatggac agcggcccag ccggacgtgc tcctcgtact cgtcacggaa
     1441 gtacttgatg gacgaatcga tcggggagac cgcgccgtcg gcgagcgcgc agaacgactt
     1501 gaacagcatg ttctgcccga cgtcgcgcat caggtcgatg tcctccatgc gcccccggcc
     1561 catctgcatc cggcggagga cccgcgtcat ccaccacgtt ccctcgcgac acggcgtgca
     1621 tttgccgcag gactcgtggg cgtagaactg ggtgaatcgc agcaccgcgt cgacgacgca
     1681 gtcggtctcg tcgagcacga tcatggcctt cgtccccagc agggagcccg ccgcctggac
     1741 ctcgtcgaac cccatctgca cgtcgaggtg gtctccggtc aggaatggcg tcgacgagcc
     1801 tccgggcgtc caggctttga tccgcttccc ctcgaggatg cccccggcat actcctcgat
     1861 cagctcgcgg gccgtcgtcc ccatgggggc ctcgtagttg ccgggacggt tgacctttcc
     1921 cgagatcgag aagacaccgg ggccggtcga cctctcggtc ccgatcgatc cgaaccactc
     1981 cgcgccccgg tcgatgatgt gcggcacgca cgagagcgtc tcgacattgt
//
LOCUS       DASPWO010000244         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_36288, whole genome shotgun sequence.
ACCESSION   DASPWO010000244 DASPWO010000000
VERSION     DASPWO010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36288"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VEO00_05445"
     CDS             complement(<1..229)
                     /locus_tag="VEO00_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HYU57476.1"
                     /translation="MARDRAAVPEAKPLRLFVAVDIPEAAKDLLIARTDRFRNRIPGA
                     RWTTPPGWHVTLKFLGATWPRLVEEVRAAVAE"
     gene            complement(316..1734)
                     /locus_tag="VEO00_05450"
     CDS             complement(316..1734)
                     /locus_tag="VEO00_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57477.1"
                     /translation="MRLRWCLALLTSGSVLAALAGPASAGAGGFTKPFPTHPAHAAPF
                     GPETIRAATSGGGRVPTNINASRKGGNQSEQTISVNPTNPNNVVIISNDEAGAGVFEA
                     YTFDGGVTWTRTEIADGDNLGNACCDAQGSFDTFGNYFFVYLGTGVQVGWSTDGGATF
                     RPAFTVATGGVDQPSLATGANSVWVSWNQSGVIKARGAKVTGLGTIGTVGGMQNVPNA
                     NGSFGDTEVGPNGEVAVVYQNPYNNEGPSTVYVNVDPDGVGGAGFGPRVTVTTTNVGG
                     FDYIPAQSGRSVDSETDLEWDRSTSAFRGRLYLIYTEETAPENNNTEILLRYSDNNGA
                     TWSGSVRVNDDATTRSQFLPKPAVDQTTGKLAVGWHDARNDAGNHGPGDTNGVANDDA
                     QFWGAVSGPGGTSFGANFQISVGTSNDNDANSGVDYGDYIGLDANAGVFWPAWADNSN
                     STGDNPNGTLNQFDVYTAKVAM"
     gene            complement(1877..>2376)
                     /locus_tag="VEO00_05455"
     CDS             complement(1877..>2376)
                     /locus_tag="VEO00_05455"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYU57478.1"
                     /translation="LIYNDEFPNESDNTEIVELYSDDNGATWSGRIRVNDDATNRSQF
                     LPWASLDQTTGWLAASWHDARMDAGDHTDGDTNGIRNDDAQFWAAVSKDGGVTWETNI
                     RVSAGTSNEDQAQNGIDYGDYTGLSFHAGVFYPIWADNSNSTGDNPNGTLNQFDLYTA
                     RVQVS"
BASE COUNT          299 a          819 c          821 g          437 t
ORIGIN      
        1 cctcggcgac ggcggccctg acctcctcga ccagtcgagg ccacgtggcg cccaggaact
       61 tgagcgtcac gtgccacccg ggcggtgtgg tccaccgggc ccccgggatg cggttccgga
      121 accgatccgt gcgcgcgatg aggaggtcct tggccgcctc agggatgtcg acggccacga
      181 agagccggag cggcttggcc tcaggtacgg cggcgcggtc gcgagccatg tcgggtccga
      241 ctgtacgaga agagggccgc tccggcgtcc gccggagcgg ccctcttcca tccgatctcg
      301 accgcgacgt ctcccctaca tcgcgacttt ggcggtgtag acatcgaact ggttcagggt
      361 tccgttcggg ttgtcccccg tgctgttcga gttgtcggcc cacgccggcc agaagacgcc
      421 cgcgttcgcg tccagcccga tgtagtcgcc gtagtccacc ccgctgttgg cgtcgttgtc
      481 gttcgacgtg cccacgctga tctggaagtt ggcgccgaag ctcgtccctc cggggccgct
      541 gaccgctccc cagaactggg cgtcgtcgtt ggccaccccg ttcgtgtcgc ccgggccgtg
      601 gttcccggca tcgttgcggg cgtcgtgcca gccgaccgcc agcttcccgg tggtctggtc
      661 caccgcaggc ttcggcagga actggctgcg ggtggttgcg tcgtcgttga ccctgacgga
      721 cccgctccac gtggcgccgt tgttgtccga gtaccggagc aggatctccg tgttgttgtt
      781 ctcgggagcg gtctcctccg tgtagatcag gtagaggcgc cctcggaagg cgctggtgct
      841 gcggtcccac tcaaggtcgg tctccgagtc gaccgaacgg ccggactgcg ccgggatgta
      901 gtcgaacccg ccgacgttgg tggtggtcac ggtgaccctg gggccgaagc cggccccgcc
      961 gaccccatcc gggtcgacgt tcacgtagac ggtcgagggc ccctcgttgt tgtaggggtt
     1021 ctggtagacc accgccacct cgccgttcgg accgacctcg gtgtccccga agctcccgtt
     1081 cgcattgggg acgttctgca tcccgccgac cgtcccgatg gtcccgaggc cggtgacctt
     1141 cgcgccgcgc gccttgatca ccccgctctg gttccagctg acccacacgc tgttcgcacc
     1201 cgtggccaga gacggctggt cgaccccgcc cgtcgccacg gtgaaggccg gccggaaggt
     1261 ggccccccca tcggtgctcc acccgacctg gaccccggtg ccgaggtaga cgaagaagta
     1321 attgccgaag gtgtcgaagg acccctgggc gtcgcagcag gcgttcccga ggttgtcgcc
     1381 gtcggcgatc tccgtccggg tccaggtgac cccgccgtcg aaggtgtagg cctcgaagac
     1441 gccggccccg gcctcgtcgt tcgagatgat cacgacgttg ttcggattgg tcgggttgac
     1501 gctgatcgtc tgctcggact ggttcccgcc cttccggctg gcattgatgt tcgtggggac
     1561 gcgccccccg ccggaggtcg cggcccggat ggtctccggt ccgaacgggg cggcatgggc
     1621 ggggtgggtc gggaacggct tggtgaaccc gccggctccg gccgaagcgg gtcccgccag
     1681 agccgcgagg accgatcccg atgtcagcaa tgccaggcac catcgcaggc gcatgagcga
     1741 accccccttg actcgaagga gtcgggcggg ccggccgccc gtcggccggc ggacggccga
     1801 ggctatccga ccgggcgggg aaccgcaatc ctgccgactg cgggccccca cccggccgtt
     1861 caccggtgtg gccggctcag ctgacctgga ccctggccgt gtacaggtcg aactggttca
     1921 gcgtcccgtt ggggttgtcg cccgtgctgt tcgagttgtc cgcccagatc gggtagaaga
     1981 ccccggcgtg gaacgacagc ccggtgtagt cgccgtagtc gatcccgttc tgggcctggt
     2041 cctcgttcga ggtgcccgcg ctgacccgga tgttcgtctc ccacgtcacg ccgccgtcct
     2101 tgctcaccgc ggcccagaac tgggcgtcgt cgttgcggat cccgttggtg tcgccgtccg
     2161 tgtggtcccc ggcgtccatc cgggcgtcgt gccatgacgc ggcgagccag ccggtcgtct
     2221 ggtccagcga ggcccacggg aggaattggc tccggttggt ggcgtcgtcg ttcacgcgga
     2281 tccggccact ccaggtcgcg ccgttgtcgt ccgagtacag ctcgacgatc tccgtgttgt
     2341 cgctctcgtt cgggaactcg tcgttgtaga tgaggt
//
LOCUS       DASPWO010000245         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_714408, whole genome shotgun sequence.
ACCESSION   DASPWO010000245 DASPWO010000000
VERSION     DASPWO010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714408"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VEO00_05460"
     CDS             complement(<1..431)
                     /locus_tag="VEO00_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HYU57479.1"
                     /translation="MRTHTCGELRASDAGGPAILAGWVARRRDHGGVIFLDLRDRDGL
                     VQVVFHPEEAPEAHAAAGAVRSEYVVRVEGEVRPRPEGTVNPNLPTGEVELAARTLEV
                     LSASETPPFPVEDDIEVDETTRLQYRYLDLRRPLMTDVLRTR"
     gene            complement(428..1690)
                     /gene="hisS"
                     /locus_tag="VEO00_05465"
     CDS             complement(428..1690)
                     /gene="hisS"
                     /locus_tag="VEO00_05465"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HYU57480.1"
                     /translation="MSAGFDPPRGTDDLLPPESDRIEALAAAAGHTARLFGFRPVETP
                     SLDHTEVFARTSGESSDVVTKEMYTFEDRAGRSLTLKPEGTASVVRAYLRHRHELPLP
                     FRGYYVERTWRYGRPQSGRLREFRQFGVEILGTDAPEADVEVIAVGDRYLRDHGLERL
                     DLRLNTIGDGACRPAYRDALLAYLRDHREELTDEHRDGFERNPLRVLDCKDAGCRAVA
                     GGAPLITDFLCEPCAAHHAAVKAGLDAWSIGYTEDPRLVRGLDYYTRTAFEFESDVLS
                     EAQATVCGGGRYDGLAEVLGGDRTPGVGFGMGLERVLMAMGKEGVPLPVARGAACYVV
                     AVGRGREAAWTLVRALRAAGVAAETTLGERPLKAQLRAADRAGAVYAAIVGDREADAG
                     VVTMRRLSDGEQQAVPREEVTAWLASRA"
     gene            complement(1698..>2128)
                     /locus_tag="VEO00_05470"
     CDS             complement(1698..>2128)
                     /locus_tag="VEO00_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYU57481.1"
                     /translation="AAATAGGELPVYVHAADEAAFTDPVGWGSGFPVTPPVPADLRTL
                     TDGRVLEFAGFSIEVQHTPGHTPGSVCFVTDAFVLSGDLVFRGTIGRSDFPHSDPEAM
                     VESLRRFLKLPDHLDVYPGHMERTTVGLERRTNPFLAGLA"
BASE COUNT          276 a          823 c          768 g          261 t
ORIGIN      
        1 cgggtccgca gcacgtcggt catcagcggg cggcgcaggt ccaggtagcg gtactgcagg
       61 cgggtcgtct cgtcgacctc gatgtcgtcc tccaccggga acggcggcgt ctccgaggcc
      121 gacaggacct cgagggtgcg cgcggcgagc tccacctcgc cggtcggcag gttcgggttc
      181 accgtcccct ccgggcgagg gcgaacctcc ccctcgaccc gcacgacgta ctcgctgcgc
      241 accgcgccgg ccgcggcgtg ggcctcgggc gcctcctcgg ggtggaagac cacctgcacc
      301 aggccgtcgc ggtcgcgcag gtcgaggaag atcaccccgc cgtggtcgcg acggcgcgcc
      361 acccacccgg ccaggatcgc cggcccgccg gcgtccgacg cacggagctc gccgcaggtg
      421 tgggtcctca ggcccgtgag gcgagccacg ccgtcacctc ctcgcgcggg accgcctgct
      481 gctcgccgtc ggacaggcgg cgcatcgtga ccactccggc atcggcctcg cggtcgccga
      541 cgatggcggc atagaccgcg ccggcccggt cggccgcgcg cagctgcgcc ttcagcgggc
      601 gctcgccgag ggtcgtctcg gccgcgacgc cggcggcccg cagcgcgcgg acgagcgtcc
      661 aggccgcctc gcgcccccgc cccacggcga ccacgtagca cgcggcgccg cgggcgaccg
      721 gcagcgggac gccctccttc cccatcgcca tcagcacccg ctccaggccc atcccgaagc
      781 ccacgcccgg cgtgcggtcg ccgccgagga cctcggccag cccgtcgtag cgcccgcccc
      841 cgcagacggt ggcctgcgcc tccgacagca cgtcgctctc gaactcgaac gccgtccgcg
      901 tgtagtaatc gagcccgcgc acgaggcgcg ggtcctccgt gtaaccgatg ctccacgcgt
      961 cgagcccggc cttcacggcg gcgtggtgcg cggcgcacgg ctcgcacagg aagtcggtga
     1021 tcagcggggc gccacccgcc accgcgcggc agcccgcgtc cttgcagtcc aggacgcgca
     1081 gcgggttgcg ctcgaagccg tcgcggtgct cgtcggtcag ctcctcgcgg tggtcgcgga
     1141 ggtaggcgag cagcgcgtcg cggtaggccg ggcggcacgc cccgtcaccg atcgtgttca
     1201 gccgcaggtc gagccgttcc aggccatggt cgcgcaggta gcggtccccg accgcgatga
     1261 cctccacgtc ggcctccggc gcgtcggtgc cgaggatctc cacgccgaac tggcggaact
     1321 cccgcagccg gcccgactgc ggtcggccgt agcgccaggt ccgctcgacg tagtacccgc
     1381 ggaacggcag cggcagctcg tggcgatgcc tcagatacgc gcggacgacc gacgcggtcc
     1441 cctccggctt cagcgtcagc gagcgaccgg cgcggtcctc gaacgtgtac atctccttcg
     1501 tcaccacatc ggagctctcc ccggaggtcc gggcgaacac ctcggtgtgg tcgagcgacg
     1561 gcgtctcgac cgggcggaag ccgaagaggc gcgcggtgtg gcccgccgcg gcggcgagcg
     1621 cctcgatccg gtcggactcg ggcggcagga ggtcgtcggt cccgcgcggc gggtcgaacc
     1681 cggcgctcac ccgcgcctca cgccaggccg gccaggaacg ggttcgtccg ccgctccagg
     1741 ccgacggtgg tccgctccat gtgcccgggg tagacgtcga ggtggtcggg gagcttcagg
     1801 aaccggcgca gggactcgac catggcctcg gggtcggagt gcgggaaatc cgagcgcccg
     1861 atcgtccccc ggaagaccag gtcccccgag agcacgaacg cgtcggtgac gaagcacacg
     1921 ctgcccgggg tgtggccggg cgtgtgctgc acctcgatcg agaacccggc gaactcgaga
     1981 acccgcccgt ctgtcagcgt gcgcaggtct gccggcaccg gcggcgtcac cgggaatccc
     2041 gagccccagc cgaccgggtc ggtgaacgcc gcctcgtcgg ccgcgtggac gtagaccggc
     2101 agctcgcccc cggcggtggc cgcggcct
//
LOCUS       DASPWO010000246         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_36424, whole genome shotgun sequence.
ACCESSION   DASPWO010000246 DASPWO010000000
VERSION     DASPWO010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36424"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..556)
                     /locus_tag="VEO00_05475"
     CDS             complement(209..556)
                     /locus_tag="VEO00_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57482.1"
                     /translation="MARRGRFLTHLLSAALLAAGCRAAPEPSASPPPVTATRPSTSPE
                     PNATTAPSAPAFHGSVAAIDAATRVRMAHTWRPGCPVGLAALRLVTLDHWGFDGRVHR
                     GELVVCRLNSDLS"
     gene            679..1029
                     /locus_tag="VEO00_05480"
     CDS             679..1029
                     /locus_tag="VEO00_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57483.1"
                     /translation="MARIGVLLVVLVALLPACSGSSVPAFCGVTDEARVAISNVPPEQ
                     YPAAAAAQVDAVRAAAEELSGDQGELARKVADDLEAASKATAGTVEFTEAYNAFVADS
                     NEFNHAYCNEPEVD"
     gene            complement(1050..1700)
                     /locus_tag="VEO00_05485"
     CDS             complement(1050..1700)
                     /locus_tag="VEO00_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYU57484.1"
                     /translation="MATRERLSASARRAQLVDVGRHVFAQRGYAATTVEEIAERAQVS
                     KPIVYEHFGGKEGLYAVIVDREMEQVVTRITEAISEGTPRERLERAALTFLHYVEENP
                     DGFAVLARDAPVSTPTGGISGLLNEVADRVGKVFANEFKRAGYDARTAPIYAHGLVGM
                     VTFVGQWWTETRKPSVKEVAGHIAALAWMGLRHLPNKPALATSVARGGDPGRGIRA"
     gene            1773..2693
                     /locus_tag="VEO00_05490"
     CDS             1773..2693
                     /locus_tag="VEO00_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HYU57485.1"
                     /translation="MLTPTDLLRELEPTVETLLARHLHAAEEWFPHEYVPWEEGRSFS
                     DDPWEPGDSTLSPIARIALELNLLTEDNLPYYHLAIWHAFGADGAWGEWSRRWTAEEG
                     RHSIAMRDLLTVTRALDPTELERARKEHVGRGYSPPGGAFSALDGLVYATLQELATRI
                     SHRGTGTITGDATADRLMRRIATDENLHYVFYRDVAATALALEPSAMMQAIGRQVLGF
                     SMPGLELPGFREKAISMAKAGIYDLRIHRDQVLMQVLFKQWRITEVEGLDDDAERARA
                     QVLSFLDDLDRTATRYEERRAEREGRSTAA"
     gene            complement(2694..>3147)
                     /locus_tag="VEO00_05495"
     CDS             complement(2694..>3147)
                     /locus_tag="VEO00_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57486.1"
                     /translation="VHDVVTEDGRVTEDTYDWYGQDERGNVWYLGEDTKEYENGEVVT
                     TAGSWEAGVDGAQAGILIPGAPEPGQSYRQEYLAGQAEDAGEVLSVSEQAEVPFGAFD
                     RVLMTRDFTPLEPDLVEHKFYAPGVGPVLALTVSGGADREELVAFERG"
BASE COUNT          464 a         1129 c         1107 g          447 t
ORIGIN      
        1 ctatcgaagc atgtcgactt gaagctcgtg agccggctgg agttcggctc gggggcagtg
       61 agtggtgatg cggtacgagc cgagaaggta gccatcgggc ttgctagccc gagccggcgc
      121 ggtgtgcgaa cgaccaagct cccccaggtc aatccgctgc ccacccgtca tctccaaagc
      181 cccagccgca gccaccatcc cacccacact acgacagatc actatttaag cgacacacca
      241 ctagctcccc tcggtggacc cggccgtcga acccccagtg gtcgagcgtg actaaccgga
      301 gggccgccag ccccaccggg cagccgggcc gccaggtgtg ggccatccgg acgcgggtgg
      361 ccgcgtcgat cgcggcgacc gagccatgga acgcgggcgc cgatggagcg gtcgtggcgt
      421 tcggctcggg agaggtggag ggccgggtcg ccgtcaccgg cggcggggag gcggagggct
      481 ccggcgccgc gcgacatccg gcggcgagga gcgccgcgga gagcaggtgc gtcaggaagc
      541 ggccgcggcg cgccatgtcg gttccatgac gcacgggatc gcggccgggt tccgacgggg
      601 gtgtccggac acggcgatag cccgcggcgc ggccgccaca ctcctgccat ccctcgtcat
      661 cgggtagcct tcccgcgcgt ggcccggatc ggagtgctcc tcgtcgtgct ggtcgcgctg
      721 ctccccgcct gcagcggcag cagcgtcccg gcgttctgcg gggtgaccga cgaggcgcgg
      781 gtcgcgatct cgaacgtgcc gcccgagcag tacccggccg cggccgcggc gcaggtggac
      841 gcggtgcgcg ccgcggccga ggaactgtcg ggcgatcagg gcgagctggc gcggaaggtc
      901 gccgacgatc tggaggccgc ctcgaaggcc acggcgggca cggtcgagtt caccgaggcg
      961 tacaacgcgt tcgtggccga ctcgaacgag ttcaaccacg cctactgcaa cgagccggag
     1021 gtcgactgag cctgcccgcg gccctccccc taggctcgga tgccgcgtcc tgggtcaccg
     1081 cctcgcgcca cgctcgtggc gagcgcgggc ttgttcggca ggtggcgcag ccccatccac
     1141 gccagcgcgg cgatgtggcc ggcgacctcc ttgaccgacg gcttgcgcgt ctccgtccac
     1201 cattggccga cgaaggtcac catgcccacc agcccgtgcg cgtagatcgg cgcggtgcgc
     1261 gcgtcatagc cggcccgctt gaactcgttc gcgaacacct tgccgacgcg gtcggccacc
     1321 tcgttcagca ggcccgagat gccgccggtg ggcgtcgaca ccggtgcgtc gcgcgcgagg
     1381 accgcgaagc cgtccgggtt ctcctccacg tagtgcagga aggtcagggc tgcgcgctcg
     1441 aggcgctcgc ggggtgtgcc ctccgagatg gcttccgtga tccgggtcac gacctgctcc
     1501 atctcgcggt cgacgatcac cgcgtacagg ccctccttgc ccccgaagtg ctcgtagacg
     1561 atcggcttcg agacctgcgc gcgctcggcg atctcctcca ccgtggtcgc ggcgtagccg
     1621 cgctgcgcga acacgtgccg cccgacgtcg acgagctgcg cgcggcgggc ggacgcgctc
     1681 aggcgttccc tggtggccat gggtttgacg aagatactac gctagcgtaa cctacgatgc
     1741 cgtaacctac gcgaacgtag ggggagattg ccatgctcac gcccaccgac ctgctccgcg
     1801 agctcgagcc gaccgtcgag acgctgctcg cgcgccacct ccacgcggcg gaggaatggt
     1861 tcccgcacga gtacgtcccg tgggaggagg gccggagctt cagcgacgac ccatgggagc
     1921 ccggcgacag cacgctctcc ccgatcgcgc gcatcgcgct ggagctgaac ctactgaccg
     1981 aggacaacct gccctactac cacctggcga tctggcacgc gttcggggcc gacggggcct
     2041 ggggcgaatg gagccgccgc tggacggccg aggaaggccg ccactcgatc gcgatgcgcg
     2101 acctgctgac ggtgacccgg gccctcgacc ccaccgagct cgagcgcgcg cggaaggaac
     2161 atgtcggccg cggctattcg ccgccgggcg gcgcattctc ggccctcgac ggcctggtct
     2221 acgcgacgct ccaggagctc gcgacgcgca tctcgcatcg cgggacgggg acgatcaccg
     2281 gtgacgcgac ggcggaccgg ttgatgcgcc ggatcgccac cgacgagaac ctgcactacg
     2341 tcttctaccg ggacgtcgcc gcgaccgcgc tggcgctcga gccgtcggcg atgatgcagg
     2401 cgatcggacg ccaggtcctc ggtttctcga tgcccggtct ggagctgccg gggttccggg
     2461 agaaggccat cagcatggcg aaggcaggca tctacgacct gcgcatccac cgcgaccagg
     2521 tgctgatgca ggtcctattt aagcagtggc ggatcacgga ggtggagggc ctcgacgacg
     2581 acgccgagcg ggcccgggcc caggtcctgt ccttcctgga cgacctcgac cggaccgcga
     2641 cgcggtacga ggagcggcgc gcggagcggg agggccgctc gaccgccgcc tgatcaccct
     2701 cgctcgaagg cgacgagctc ctcgcggtcg gcgccgccgg acacggtcag ggcgagcacc
     2761 ggcccgacgc ccggggcgta gaacttgtgc tcgacgaggt cgggctcgag cggcgtgaaa
     2821 tccctggtca tcagcacgcg gtcgaaggcg ccgaacggca cctcggcctg ctccgacacg
     2881 ctgagcacct caccggcgtc ctcggcctgc ccggcgaggt actcctggcg atagctctgg
     2941 cccggctccg gggcccccgg gatcaggatg cccgcctgcg ccccgtcgac gccggcctcc
     3001 cacgagccgg cggtggtgac gacctcgccg ttctcgtact ccttcgtgtc ctcgccgagg
     3061 taccagacgt tcccgcgttc gtcctggccg taccagtcgt acgtgtcctc cgtcacccgg
     3121 ccgtcctcgg tgacgacgtc gtgcacg
//
LOCUS       DASPWO010000247         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1137110, whole genome shotgun sequence.
ACCESSION   DASPWO010000247 DASPWO010000000
VERSION     DASPWO010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137110"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VEO00_05500"
     CDS             complement(<1..557)
                     /locus_tag="VEO00_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HYU57487.1"
                     /translation="MATAEEFSLDPEEIASALRKYVEEYRPDTAREEVGRVLETSDGI
                     ARVAGLPRTMANELLLFEGGVLGLALNLDEREIGCVVLGNAAEVEEGGAVKQTGRILS
                     VPVGDAFLGRVTDPLGRAIDGKGEIGPQETRALEIQAPSVVQRQPVKEPLQTGIKAID
                     AMTAIGRGQRELIIGDRQTGKTAIAV"
     gene            complement(563..1105)
                     /gene="atpH"
                     /locus_tag="VEO00_05505"
     CDS             complement(563..1105)
                     /gene="atpH"
                     /locus_tag="VEO00_05505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HYU57488.1"
                     /translation="MADGGDAVRGYAQAIFQIAEAEGALEAVEDELFRFAKALESEDR
                     LRDALSDPVLPAERKKAVLAKVLGGRANPHTVSILGFLVEQGRGRELARIIEELARVA
                     AARRNRAVAEVRTAVPLSDEQRERLSAALSKATGKTVELKVLVDPAVIGGVLARVGDQ
                     VIDGTVRRRLELARERLGGV"
     gene            complement(1098..1733)
                     /gene="atpF"
                     /locus_tag="VEO00_05510"
     CDS             complement(1098..1733)
                     /gene="atpF"
                     /locus_tag="VEO00_05510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HYU57489.1"
                     /translation="MHALIVGFASVLAQAEEEVSEPKDLYPHAEELIVGALAFTILFV
                     FMAKFVIPRVNTMIEERRTRIQGELEKAESTRSEADQVLGQYREQLANARAESNRIIE
                     EARKTAEEMRRDLLERARQEGQQVVAKARDEIRAERDRAFADLKRQVGELSVELATRV
                     VGESLDRERQVRMVEQYIEEVAAMDAPPEQGNGADPFSAWRGDEGSGETDG"
     gene            complement(1744..1980)
                     /gene="atpE"
                     /locus_tag="VEO00_05515"
     CDS             complement(1744..1980)
                     /gene="atpE"
                     /locus_tag="VEO00_05515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007447723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HYU57490.1"
                     /translation="MLGLLAATTGDVSSIGKGLAYGLAAIGPGIGIGILMGKSVEAMA
                     RQPEAAGMVRGTMFIGIGLIEALALFGFVLSFII"
     gene            complement(2018..>2375)
                     /locus_tag="VEO00_05520"
     CDS             complement(2018..>2375)
                     /locus_tag="VEO00_05520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HYU57491.1"
                     /translation="IRRHGLFGYLKIIMFPPGAPWPIYILLSPIELLQVVVVRPLTLS
                     VRLFANMVAGHFLLAVFFLGSLALVTGIPYVLGIVSFGIGVALVGFEIFVASLQAFIF
                     SMLTASYIAGAMAEEH"
BASE COUNT          343 a          900 c          779 g          353 t
ORIGIN      
        1 accgcgatgg ccgtcttgcc ggtctggcgg tccccgatga tcaattcgcg ctggccgcgc
       61 ccgatggccg tcatcgcgtc gatggccttg atcccggtct gcagcggctc cttcaccggc
      121 tggcgctgca ccaccgacgg ggcctggatc tcgagggcgc gggtctcctg gggcccgatc
      181 tcccccttgc cgtcgatcgc ccggccgagc gggtcggtga cccggccgag gaacgcgtcc
      241 ccgacgggca ccgacaggat gcgcccggtc tgcttgaccg cgccgccctc ctcgacctcc
      301 gccgcgttgc cgaggaccac gcacccgatc tcgcgctcgt cgaggttcag ggccagcccg
      361 agcacaccgc cctcgaacag gagcagctcg ttggccattg tccgcggcag gccggcgacg
      421 cgggcgatcc cgtcgctggt ctcgaggacg cgcccgacct cctcgcgcgc ggtgtcgggg
      481 cgatactcct cgacgtactt ccggagcgcc gaggcgatct cttccgggtc gagcgagaat
      541 tcttcggccg tcgccatgtc cgtcacacgc ctccgagtcg ttcgcgggcc agctccaggc
      601 gccggcggac ggtcccgtcg atcacctggt cgccgacccg ggccaggacc ccgccgatca
      661 cggcgggatc caccagcacc ttcagctcga ccgtcttccc ggtcgccttc gacagcgccg
      721 ccgacagccg ctcgcgctgc tcgtccgaga gcggcacggc ggtgcgcacc tcggccaccg
      781 cgcggttgcg ccgcgccgcc gccacccgcg ccagctcctc gatgatccgg gccagctccc
      841 ggccgcggcc ctgctcgacc aggaacccga ggatcgagac cgtgtgcggg ttggcccggc
      901 cgccgagcac tttcgcgaga acggccttct tgcgctcggc cggcaggacc gggtcggaca
      961 gggcgtcgcg caggcggtcc tcgctctcca gagccttggc gaagcggaag agctcgtcct
     1021 cgacggcctc gagggcgccc tcggcctcgg cgatctggaa gatcgcctgg gcgtagccgc
     1081 ggaccgcgtc gccgccgtca gccatccgtt tctcccgaac cctcgtcgcc gcgccaggcg
     1141 ctgaaggggt ccgcgccgtt cccctgctcg ggtggcgcgt ccatggcggc gacctcctcg
     1201 atgtactgct cgaccatccg cacctggcgc tcgcgatcga gggactcgcc cacgacgcgc
     1261 gtcgccagct cgaccgacag ctcgccgacc tggcgcttca ggtcggcgaa ggcgcggtcg
     1321 cgctcggccc ggatctcgtc gcgggccttg gccaccacct gctgcccctc ctgccgggcg
     1381 cgctcgagca ggtcgcggcg catctcctcg gcggtcttgc gcgcctcctc gatgatccgg
     1441 ttcgactccg cccgcgcgtt cgccagctgc tcccggtact ggcccagaac ctggtcggcc
     1501 tcgctccggg tggactcggc cttctccagc tcgccctgga tccgcgtgcg ccgctcctcg
     1561 atcatcgtgt tcacgcgcgg gatgacgaac ttggccatga agacgaacag gatcgtgaac
     1621 gccagggcgc cgacgatcag ctcctcggca tgcgggtaga ggtccttcgg ttcgctgacc
     1681 tcctcctcgg cctgcgccag gacggaggcg aacccgacga tcagcgcgtg cacggccgcc
     1741 ccctcagatg atgaacgaca ggacgaagcc gaagagggcc agcgcctcga tcagcccgat
     1801 gccgatgaac atggtccccc ggaccatgcc agcggcctcc ggctgccggg ccatcgcctc
     1861 caccgacttg cccatcagga tgccgatgcc gatgccgggc ccgatggcgg ccagaccgta
     1921 ggccagcccc ttcccgatcg agctgacgtc gccggtggtc gcggccagca gccccagcac
     1981 gtgcgtcacc actccaacca tgtctcctcc ttgagcgtca atgctcctcc gccatcgctc
     2041 ccgcgatgta cgacgccgtg agcatgctga agatgaacgc ctgcaggctc gcgacgaaga
     2101 tctcgaatcc gaccagggcc acgccgatgc cgaacgagac gatgccgagc acgtagggga
     2161 tgccggtgac gagagcgagg gaccccagga agaacacggc cagcaggaag tgcccggcca
     2221 ccatgttggc gaagagtcgg acggagagcg tgaggggccg gaccacgacc acctggagca
     2281 gctcgatggg cgacagcagg atgtagatgg gccacggcgc gcccggcggg aacatgatga
     2341 tcttcaggta cccgaacaac ccgtgccgcc tgatg
//
LOCUS       DASPWO010000248         5648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1221927, whole genome shotgun sequence.
ACCESSION   DASPWO010000248 DASPWO010000000
VERSION     DASPWO010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221927"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VEO00_05525"
     CDS             <1..836
                     /locus_tag="VEO00_05525"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYU57492.1"
                     /translation="GCEYASIFIALGARVTLVQGEARLVPFLDGEISDLLAEVLLQEG
                     MDLRLGVKARAVERANGMVQVTLEDGAVVKPNTVLVAMGTVGNSAGLGLEEAGVEVDA
                     RGRIIVDDEYRTTAEGVYAAGDVIGSPALASTGMEQGRVAVCHAFGIPFKEAVDPHAP
                     YGVYTIPEVAMAGLTEEAARAEGLDVAVGRGRFSENPRARIAGFGEGMVKLVFRRSDH
                     ALVGVHVLGEMASELVHVGQLAIAEGLPIDRFIQATFNVPTRTDAYKYAAYDGLQNLA
                     D"
     gene            complement(837..1985)
                     /locus_tag="VEO00_05530"
     CDS             complement(837..1985)
                     /locus_tag="VEO00_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HYU57493.1"
                     /translation="MDERAERERRYERGDDAWPLGSAEWEAAARDALTREAFDYLAGG
                     AGEESTMEANRAAFERWRLAPRMLTGNAERDLSVEVLGTRSPVPFLLAPIGVQGIMHP
                     DGELATADAAAATRVPMVVSSAASRSTEETAARLGEAPWWFQLYWVNDREVAASFVSR
                     AEAAGARAIVLTVDTLTIGFRDRDQRNSAYLPFLEGEGIGQFVSDPVFRSRLSVPPEE
                     DMAAAGMAMVAMFPHLALSWDDLGWLRERTGLPVLLKGILRGDDAVRARDAGAAGVVV
                     SNHGGRQVDGEIAALDALPEVRDALGDAFPVLVDSGVRRASDILKALALGANAVLLGR
                     PYAWGLAAGGSAGVRRVIETLRGELDVVTALCGLRRPTEADRDLLRPV"
     gene            complement(2027..3598)
                     /locus_tag="VEO00_05535"
     CDS             complement(2027..3598)
                     /locus_tag="VEO00_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU57494.1"
                     /translation="MPFEGENRREKIVVLLTMCFALFMAMLDNTVVNVALPTLSRDLD
                     AGVSSLQWIVDGYVLAFASLLLTGGILGDRYGRKRMFLTGLGLFTLASLLCGLSANTG
                     QLIGFRALQGVGAAMLMPGTLSILTVTFPAHERARAIGIWAGVSGLALALGPTAGGLL
                     VEHVGWESVFFLNVPIGVVGFLVAWRVVRESRSEEERHLDIAGLLLGTGALFSVTYAL
                     IEANQLGWGDARIVGSLVAAAVLGVGFLAWERRSPHAMLPLRFFRIPAFSAGNSVAFS
                     MSLGMFAIFFFLSLYMQLIRGYSPFQAGIRFLPLTLMIFIVAPQAGHFAQRHGSRWPM
                     TFGLALAGSGLLLLSRVDATTSFALIFPVFVMMGAGIASTMAPMTAAVMNAVGPQRAG
                     LGSATTNTSREVGGVFGIALLGTLLTTKLKSSLTAAIAGLGLGAAQESAIVAAAGHGR
                     LDPALLQGLRPDQAGAVQQAFGAAFIDGFHLALLVASLVLFAAALIAFRFIPSGGPRV
                     EVAEAGAPEAAAAAH"
     gene            complement(3640..4680)
                     /locus_tag="VEO00_05540"
     CDS             complement(3640..4680)
                     /locus_tag="VEO00_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYU57495.1"
                     /translation="MTYGKVLVGTDGSETAQRAVRAAIGMARSVYGELLVAVAYDPGH
                     TAKQTAWKVLDAALAEARAEGLTARGWAKPGDPLEVITEVAEQPGADLVVTGNKGMGR
                     PRRVFLGSVPERLAHAAPSDLLIVRTGSERGTDGRYRSILVGTDGSPTATEAVRTTFA
                     LARTLECPVTVVHVGDEAFGRIVLKDVAEKLGQHVEVRARAVEGDPAERICDVAEEED
                     VDLIVVGNRGMEGAQRYLLPAIPNKVAHYATTDVLIVRTVGRSAKDLVPGEGGVVTLD
                     GQRVAAYRDADGTLHAVSARCTHLGCTVGWNPGEGTWDCPCHGSRYHADGTVLRGPAE
                     KALTPVDLSGKG"
     gene            complement(4697..5173)
                     /locus_tag="VEO00_05545"
     CDS             complement(4697..5173)
                     /locus_tag="VEO00_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57496.1"
                     /translation="MTAPELREEWLLVHIAGVLVFVLAHGVSAGVGLRLRKEREPGRI
                     DALLQLSSTALRWMYLGLLLLLAGGILSGFVIKAWRHAWIWVALGTLVTEMIAMYVIA
                     TRYYQRVRKVLQMRLGAESATEDVASVMSSTRPIVLAVVGYAGLFFIAYLMVFKPF"
     gene            complement(5217..>5648)
                     /locus_tag="VEO00_05550"
     CDS             complement(5217..>5648)
                     /locus_tag="VEO00_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYU57497.1"
                     /translation="FDLKVVGWGVRKIHQIPIRRGMADRAALREAAAVIRDGALAGIF
                     PEGRVSEDASLARLQKGAARLALTAAVPLVPMGIWGTNLRWPRGGLRLGLPLRPRVAI
                     VVGEPIAVAGDPRSAADVRALTVQIAERLGELAAVARRAAR"
BASE COUNT          846 a         2123 c         1993 g          686 t
ORIGIN      
        1 tcggctgcga gtacgcctcg atcttcatcg cgctcggcgc gcgggtcacg ctcgtccagg
       61 gcgaggcccg tctggtcccg ttcctcgacg gcgagatctc ggacctgctg gccgaggtcc
      121 tgctgcagga ggggatggac ctccgtctgg gggtcaaggc ccgcgcggtc gagcgcgcga
      181 acgggatggt ccaggtgacc ctggaggacg gcgcggtggt caagcccaac accgtgctcg
      241 tggcgatggg gacggtcggg aactcggccg gcctcggcct ggaggaggcg ggggtcgagg
      301 tggacgcccg ggggcggatc atcgtggacg acgagtaccg cacgaccgcc gagggcgtct
      361 acgcggccgg ggacgtcatc ggatcaccgg cgctggcctc gaccggcatg gagcaggggc
      421 gcgtggccgt gtgccacgcg ttcggcatcc cgttcaagga ggcggtcgac ccgcacgcgc
      481 cgtacggcgt gtacacgatc cccgaggtgg cgatggccgg cctgaccgag gaggccgccc
      541 gggcggaggg gctcgacgtg gcggtcggac gggggcggtt ctcggagaac ccccgggcgc
      601 ggatcgccgg gttcggcgag ggcatggtca agctcgtgtt ccgtcgctcc gaccacgcgc
      661 tggtcggggt gcacgtgctc ggcgagatgg cctcggagct ggtccacgtc gggcagctgg
      721 cgatcgccga gggcctgccg atcgaccgct tcatccaggc gaccttcaac gttccgacgc
      781 gaaccgacgc ctacaagtac gccgcctacg acggcctgca gaacctggca gactgatcag
      841 accgggcgca gcagatcgcg atccgcctcg gtggggcggc gcaggccgca gagcgcggtg
      901 acgacgtcga gctcgccgcg cagcgtctcg atcacccggc ggacacccgc gctgccgccg
      961 gcggccaggc cccaggcgta cgggcggccg agcagcaccg cgttcgcgcc gagggccagg
     1021 gccttcagga tgtccgacgc gcgccggacg ccggagtcca ccagcaccgg gaacgcatcg
     1081 ccgagcgcgt cgcgcacctc gggcagcgcg tcgagcgcgg cgatctcacc atcgacctgc
     1141 cgtccgccgt ggttcgagac caccaccccc gcggcgccag cgtcgcgcgc ccggaccgcg
     1201 tcgtcgccgc gcaggatccc cttcagcagc acgggcagcc ccgtgcgctc gcgcagccag
     1261 ccgaggtcgt cccaggacag cgcgaggtgc gggaacatcg ccaccatcgc catgccggcg
     1321 gcggccatgt cctcctcggg cggaaccgat agtcggctgc gaaagaccgg gtcgctgacg
     1381 aactgcccga tcccctcgcc ctcgaggaag gggaggtagg cgctgttgcg ctggtcacgg
     1441 tcccggaacc cgatcgtcag ggtgtcgacg gtcagcacga tcgcacgcgc ccccgccgcc
     1501 tccgcccggg aaacgaagct ggccgccacc tcgcggtcgt tgacccagta gagctggaac
     1561 caccacggcg cctcgccgag ccgtgcggcg gtctcctccg tcgagcggga cgcggcgctc
     1621 gacaccacca tcggcactcg ggtcgccgcg gcggcgtccg ccgtcgccag ctcgccgtcc
     1681 gggtgcatga tcccctgcac cccgatcggc gccaggagga acggcaccgg cgagcgggtg
     1741 ccgagcacct ccaccgacag gtcgcgctcg gcgttgccgg tcagcatccg cggcgcgagg
     1801 cgccagcgct cgaaggccgc gcggttcgcc tccatcgtgg attcctcgcc ggcgcccccg
     1861 gccaggtagt cgaacgcctc gcgcgtcagg gcgtcgcggg cggcggcctc ccactccgcc
     1921 gacccgagcg gccaggcatc gtcgccgcgc tcgtaccgcc gctcgcgctc ggcccgctcg
     1981 tccatgcggg cgaagcctat cgccggggcg cgggatcagc ggtcgttcag tgggcggcgg
     2041 ccgcggcttc gggggcgccg gcttcggcca cctcgacgcg ggggccgccg gaggggatga
     2101 agcggaacgc gatcagcgcc gcggcgaaga gcaccaggct ggccacgagc agggcgaggt
     2161 ggaagccgtc gatgaacgcg gcgccgaagg cctgctgcac cgccccggcc tgatcgggcc
     2221 gcagcccctg cagcagggcg ggatcgagcc ggccgtgccc ggccgcggcc acgatggcgc
     2281 tctcctgcgc cgcgccgagg ccgagcccgg cgatcgcggc cgtgagcgag gacttcagct
     2341 tggtggtcag gagcgtcccg aggagggcga tcccgaacac gccgccgacc tcgcggctgg
     2401 tgttcgtggt cgccgacccc aggccggcgc gctgcgggcc gaccgcgttc atcaccgccg
     2461 ccgtcatcgg cgccatcgtc gaggcgatcc cggcgcccat catcacgaac accgggaaga
     2521 tcagcgcgaa cgacgtggtg gcgtccaccc ggctgagcag cagcagcccg ctccccgcca
     2581 gcgccagccc gaaggtcatc ggccagcggc tgccgtggcg ctgcgcgaag tgccccgcct
     2641 gcggcgcgac gatgaagatc atcagcgtca gcggcaggaa gcggatcccc gcctggaacg
     2701 gcgagtagcc ccggatcagc tgcatgtaca ggctgaggaa gaagaagatc gcgaacatcc
     2761 ccagcgacat cgagaaggcc acgctgttgc ccgcgctgaa cgcggggatg cggaagaagc
     2821 gcagcggcag catggcgtgc gggctgcgcc gctcccacgc caggaacccc acgccgagca
     2881 ccgcggcggc caccaggctc ccgacgatgc gcgcgtcgcc ccacccgagc tggttcgcct
     2941 cgatcagggc gtaggtcacc gagaagagcg ctccggtgcc gagcagcagg ccggcgatgt
     3001 ccaggtggcg ctcctcctcg gagcgcgact cgcgcaccac gcgccaggcc accaggaacc
     3061 cgacgacgcc gatcggcacg ttcaggaaga acaccgactc ccacccgacg tgctcgacga
     3121 gcagcccacc ggcggtcggt ccgagcgcca gggccaggcc ggacacgccg gcccagatgc
     3181 cgatcgcccg cgcccgctcg tgcgccggga acgtcacggt caggatcgac agggttcccg
     3241 gcatgagcat cgccgcgccg acgccctgca gggcgcggaa cccgatcagc tgcccggtgt
     3301 tcgccgacag cccacagagc agggaggcga gcgtgaacag gccgagaccg gtcaggaaca
     3361 tccgcttgcg cccgtagcgg tcgccgagga tgccgccggt cagcagcagg ctggcaaagg
     3421 ccaggacgta cccgtcgacg atccactgca ggctgctgac gccggcgtcc agatcgcgcg
     3481 acagggtggg cagggcgacg ttcacgacgg tgttgtcgag catcgccatg aacagggcga
     3541 agcacatcgt cagcaggacg acgatcttct cgcggcggtt ctctccctcg aacggcatcg
     3601 gttcagtatg ccgggatcac gagaaatggc cggcggtcgt cagcccttcc cggagagatc
     3661 caccggtgtc agggccttct cggccgggcc gcggagcacg gtgccgtcgg cgtggtaccg
     3721 ggagccgtgg caggggcagt cccaggtccc ctcgccgggg ttccacccca ccgtgcagcc
     3781 gaggtgcgtg caacgcgccg acaccgcgtg cagggtcccg tccgcgtcgc ggtaggccgc
     3841 cacccgctgg ccatcgagcg tcaccacccc gccctccccg ggcacgaggt ccttcgccga
     3901 acgccccacc gtgcgcacga tcagcacgtc ggtggtcgcg tagtgggcga ccttgttcgg
     3961 gatcgccggc agcaggtacc gctgcgcgcc ctccatcccg cggttcccga ccacgatcag
     4021 gtcgacgtcc tcctcctcgg ccacgtcaca gatgcgctcg gccgggtcgc cctccacggc
     4081 gcgggcccgc acctcgacgt gctgcccgag cttctcggcg acgtccttca gcacgatgcg
     4141 cccgaacgcc tcgtcgccga catgcaccac cgtcacgggg cactcgagcg tccgggccag
     4201 cgcgaacgtc gtgcgcacgg cctcggtcgc ggtcggcgag ccgtcggtcc cgaccaggat
     4261 cgagcggtac cggccgtcgg tcccccgttc ggacccggtg cgcacgatca gcaggtcgct
     4321 cggcgcggcg tgcgccagcc gctccggaac gctgccgagg aagacccgcc gcggccggcc
     4381 catccccttg ttcccggtga cgaccaggtc ggcgcccggc tgctcggcga cctcggtgat
     4441 gacctcgagc ggatcgcccg gcttcgccca cccgcgggcc gtcagcccct cggcccgcgc
     4501 ctcggcgagc gcggcgtcga gcaccttcca ggcggtctgc ttggcagtgt ggccgggatc
     4561 ataggccacc gccaccagca gctcgccgta caccgagcgg gccatcccga tcgcggcgcg
     4621 caccgcccgc tgggccgtct ccgagccgtc cgtaccgacg aggaccttgc cgtacgtcac
     4681 cgcgcgtgca ccgccatcag aagggcttga acaccatcag gtaggcgatg aagaacaggc
     4741 cggcgtagcc gaccacggcc agaacgatcg ggcgggtcga ggacatgacc gaggccacgt
     4801 cctcggtcgc cgactcggcg cccagccgca tctgcagcac cttgcggaca cgctggtagt
     4861 accgcgtcgc gatcacgtac atcgcgatca tctccgtgac gagcgttccc agcgcgaccc
     4921 agatccacgc gtgcctccag gccttgatca cgaacccgct gaggatgccc ccggccagca
     4981 gcagcagcag gccgaggtac atccaccgca gcgcggtcga cgacagctgc agcagggcat
     5041 cgatccggcc cggctcgcgc tccttgcgca gccggaggcc gacccccgcg gacaccccgt
     5101 gcgccagcac gaacaccagc acgcctgcga tatgcacgag cagccactcc tcacgcagtt
     5161 cgggcgcggt caacgatgcg atcacacgga cctcggctcg gccggaaggc ggaatgctac
     5221 cgcgcggccc ggcgggcgac cgccgccagc tccccgagcc gctccgcgat ctgcaccgtc
     5281 agcgcgcgga cgtccgcggc ggagcggggg tcgccggcga ccgcgatggg ctccccgacc
     5341 acgatggcga ctcgcgggcg cagcggcagg cccaatcgga ggcccccgcg cggccagcgc
     5401 aggttcgtcc cccagatccc catcgggacg agcgggacgg cggcggtcag cgccagccgg
     5461 gcggcgccct tctgcagccg cgccaggctg gcgtcctccg acacccggcc ctcggggaag
     5521 atccccgcca gcgccccgtc gcggatcacg gcggcggctt ccctcagcgc ggcgcggtcg
     5581 gccatccccc ggcggatcgg gatctgatgg atcttgcgca ctccccaccc gaccaccttg
     5641 agatcgaa
//
LOCUS       DASPWO010000249         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_547821, whole genome shotgun sequence.
ACCESSION   DASPWO010000249 DASPWO010000000
VERSION     DASPWO010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547821"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /gene="infC"
                     /locus_tag="VEO00_05555"
     CDS             complement(<1..573)
                     /gene="infC"
                     /locus_tag="VEO00_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007263145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HYU57498.1"
                     /translation="MNDRIRAPEVRLVGADGAQIGIVPIQEALGLAREQDLDLVEVAP
                     QANPPVCRIMDYGKFKYERDVRQKEARKKQSRIDVKEIKFRPKIDRHDYDTKKGHVVR
                     FLQAGARVKVTIMFRGREMAHTELGRKILDRLVEDLKDIAVVDTIPKLDGRNMVMVIA
                     PIRRPAPPKVRETPNGETRPAEAPAQAPAAE"
     gene            complement(703..909)
                     /locus_tag="VEO00_05560"
     CDS             complement(703..909)
                     /locus_tag="VEO00_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HYU57499.1"
                     /translation="MAQGTVKWFSNEKGYGFIARPDGDDVFVHFTAIAGDGYRTLTEG
                     QEVEFDVVDGPKGKQAANVRPVGG"
     gene            complement(1058..1408)
                     /locus_tag="VEO00_05565"
     CDS             complement(1058..1408)
                     /locus_tag="VEO00_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HYU57500.1"
                     /translation="MIVEKPWGKVTTYALNQPSSVRIITIEPGQETSEHYHQLRDEMW
                     VVLDPGLRIQIGNRQVDAKPGQEFVVSAEEPHRITNTGAERGRVLEVAHGYTTEDDTF
                     RLQDDYGRSLEPEW"
     gene            complement(1405..1899)
                     /gene="ribH"
                     /locus_tag="VEO00_05570"
     CDS             complement(1405..1899)
                     /gene="ribH"
                     /locus_tag="VEO00_05570"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009655927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HYU57501.1"
                     /translation="MTEYRWSGAARGRRFAIVAARFNRVVVERLVEGARACLTKHGVD
                     GEDVDVAWVPGAFEIPQAAVALARSGEYDAIVCLGAVVRGETAHFDFVAAEAARGIAA
                     VGRATGVPATFGVLTTESLEQALDRAGGRHGNKGWDAAQAAIEMAGLLDNLPGKNRMV
                     GGET"
     gene            complement(1896..>2841)
                     /gene="ribA"
                     /locus_tag="VEO00_05575"
     CDS             complement(1896..>2841)
                     /gene="ribA"
                     /locus_tag="VEO00_05575"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009678163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HYU57502.1"
                     /translation="RAATILRACHPDARAEDFLMPGHVFPLRAQEGGVLRRAGHTEAA
                     VDLARLAGLSPAGVICEIMHPDGSMARLEELQRVAAEHGLRMVSIADLIQFRRRTEVL
                     VERVATARIPTEYGEFNASAYQSRVDGRVHVAMVLGDVGDGQGVMVRVHSECLTGDVF
                     GSLRCDCGPQLSAAFAHIGREGRGVVLYIRGHEGRAIGLTHKLRAYALQEEGRDTVEA
                     NVELGFPPDPRDYGIGAQILRDVGVRTMRLLTNNPAKRAGLEGWGLSIVERIPLQTDP
                     TEENIEYLRTKREKLGHLIDNLGLPTPPADAAGPAERM"
BASE COUNT          373 a         1075 c          943 g          450 t
ORIGIN      
        1 ctcggctgcc ggcgcctgcg ccggcgcctc cgccggccgg gtctccccgt tcggggtctc
       61 ccgcaccttc ggcggggcgg gccttcggat cggcgcgatg accatcacca tgttccggcc
      121 atcgagcttc gggatcgtgt ccaccaccgc gatgtccttc agatcctcca ccaggcggtc
      181 caggatcttg cggccgagct ccgtgtgcgc catctcgcgc ccccggaaca tgatcgtcac
      241 cttgacccgg gccccggcct gcaggaaccg aacgacgtgg cccttcttcg tgtcgtagtc
      301 gtgccggtcg atcttcgggc ggaacttgat ctccttcacg tcgatccgcg actgcttctt
      361 gcgggcctcc ttctgccgga cgtcgcgctc gtacttgaac ttgccgtaat ccatgatcct
      421 gcagacgggc gggttggcct ggggcgccac ctccaccagg tcaaggtcct gttccctggc
      481 cagcccgagc gcctcctgga tcgggacgat gccgatctga gcgccgtccg caccgaccag
      541 gcgcacctcg ggagcgcgga tccggtcgtt tatccgcgga tcaaccgaga ccgagcatca
      601 cccccagaca cgtcatgccg ctcgccgggc gagcggcagg ttccaacgac ttcgagagta
      661 taggggtgcc gcggggagcc gccagatgca gccgtccgtc gctcagcccc cgaccggccg
      721 cacgttggcg gcctgcttcc ccttcgggcc gtccacgacg tcgaactcga cctcctggcc
      781 ctcggtgagg gtgcggtagc cgtcgccggc gatcgcggtg aagtggacga acacgtcgtc
      841 cccgtccgga cgggcgatga acccgtagcc cttctcgttg ctgaaccact tgacggtgcc
      901 ctgcgccaac gtctcgacct cctggtgcgc gccccgccgg agagcgggga aaccgcgcga
      961 gactaccacc gccgggggtt tggggggtag ctcgccctgg ggagcgatgc gaccgacccc
     1021 cgacggcgag cgaaccccca aggcgggacg ctcgtggtta ccactccggt tccagtgagc
     1081 ggccgtagtc gtcctgcagg cggaacgtgt cgtcctccgt cgtgtagccg tgggccacct
     1141 cgaggacgcg cccacgctcg gctccggtgt tcgtgatccg gtgcggctcc tccgcggaga
     1201 cgacgaactc ctggcccggc ttggcgtcca cctggcggtt gccgatctgg atgcgcaggc
     1261 ccgggtccag cacgacccac atctcgtccc gcagttggtg gtagtgctcg ctggtctcct
     1321 gcccgggctc gatcgtgatg atccgcaccg acgacggctg gttcagcgcg tacgtcgtga
     1381 ccttccccca cggcttctcg acgatcacgt ctcgcccccc accatcctgt tcttccccgg
     1441 gaggttgtcg agcagacccg ccatctcgat ggcggcctgg gccgcgtccc accccttgtt
     1501 gccgtgccgg ccgccggccc tgtcgagcgc ctgctccagg ctctcagtcg tcagcacgcc
     1561 gaacgtcgcc gggacgccgg tcgcgcgccc gaccgccgcg atgcccctgg ccgcctcggc
     1621 ggccacgaag tcgaaatggg ccgtttcgcc gcggaccacc gcccccaggc agacgatggc
     1681 gtcgtactcg ccggagcgtg cgagcgccac tgccgcctgc gggatctcga acgcgcccgg
     1741 gacccaggcc acgtcgacgt cctcgccgtc gacgccgtgc ttcgtcaggc aggcccgggc
     1801 gccctcgacc agccgctcga ccaccacccg gttgaagcgg gccgcgacga tcgcgaagcg
     1861 gcgcccgcga gccgcgcccg accaacggta ctcggtcaca tccgctccgc cggaccggcg
     1921 gcgtcggccg gcggcgtggg cagcccgagg ttgtcgatca ggtggccgag cttctcgcgc
     1981 ttcgtgcgca ggtactcgat gttctcctcg gtcgggtcgg tctgcagcgg gatgcgctcc
     2041 acgatcgaga gcccccaccc ttccagcccg gcgcgcttgg ccggattgtt cgtcagcagg
     2101 cgcatcgtcc gcacccccac gtcgcgcagg atctgggcgc cgatcccgta atcgcgcggg
     2161 tccggcggga acccgagctc cacgttcgcc tcgacggtgt cccggccttc ctcctgcagc
     2221 gcgtacgcgc gcagcttgtg ggtcaggccg atcgcgcggc cctcgtgccc gcggatgtag
     2281 agcaccaccc cgcggccctc gcgaccgata tgggcgaacg ccgccgagag ttgcgggccg
     2341 cagtcgcagc gcagcgaccc gaacacgtcg ccggtcagac actccgagtg gacgcggacc
     2401 atgacgccct ggccgtcgcc gacgtcgccg agaaccatgg cgacgtggac ccggccgtcg
     2461 acccggctct ggtaggcgga ggcgttgaac tcgccgtact ccgtgggaat cctggcggtg
     2521 gcgacccgct cgaccagcac ctccgtgcgc cggcggaact ggatgaggtc ggcgatcgac
     2581 accatccgca gcccgtgctc cgccgccacc cgctgcagtt cctccaggcg ggccatgctg
     2641 ccgtcggggt gcatgatctc gcagatcacg ccggctggtg agagccccgc caggcgagcg
     2701 aggtcgaccg cggcctcggt atggccggcc cggcgcagca cgccgccctc ctgcgcccgc
     2761 agtgggaaga cgtgccccgg catcaggaag tcctcggccc gggcgtcggg gtggcacgcg
     2821 cgcaggatcg tcgccgcgcg g
//
LOCUS       DASPWO010000250         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1057966, whole genome shotgun sequence.
ACCESSION   DASPWO010000250 DASPWO010000000
VERSION     DASPWO010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057966"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VEO00_05580"
     CDS             complement(<1..257)
                     /locus_tag="VEO00_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 39"
                     /protein_id="HYU57503.1"
                     /translation="MRVRGRLTLAIATGLLVVPVAALAAKHNGHQHAPGPGGGHPSQP
                     WHPGAQGGHPGAPGGHPGSQGDHQPGGQDGGPGGQPGGPTPG"
     gene            376..1281
                     /locus_tag="VEO00_05585"
     CDS             376..1281
                     /locus_tag="VEO00_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57504.1"
                     /translation="MTAAADPPSAATAPPSVWSIASKMVGGALRSTPAKVIGAVIAAL
                     VLLGAVLSGIANGIGGFFGTLLVALFVLGVVGFIVAVPVFVVVLFIASSRARSAESSA
                     MQQYALTRRLTYSPSGTLLEATPLLSAGTSRQTQDVMTGLLPGGLQGTLAHYTYYVRH
                     HTQQGSHRVPYPNTVVIAQIPESTAYVPKLLCHDGRRIGGANIFGLDLSGDAEVELES
                     TALNERYSIRTAAGQDQAWLRELFTPVFIDWLAAKAAAEFSFELVNGLLCVSIAGRLD
                     RADQLDWLCGAATYVAGRIRGEVTE"
     gene            complement(1437..2099)
                     /locus_tag="VEO00_05590"
     CDS             complement(1437..2099)
                     /locus_tag="VEO00_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57505.1"
                     /translation="MTQDRVHANRVAHIAIGVCVGVALLMIGLVGSASARDVNSDHIP
                     DQWEKHYDLSLKVNQAKRDQDGDQMRNRAEYHAGTNPREDDSDGDGVPDADENAGEVS
                     SFTPGTEEGTGTLVIDLYAGGTLSGEVTAETRIICAPEGDEEQTAMFAPQPPAEGTGP
                     SGTSQQPPEGGALPSGDGEHNCPEGTECSTDDLVPGTIVHEATVEVSANGNEFEKVVL
                     GS"
BASE COUNT          298 a          847 c          800 g          393 t
ORIGIN      
        1 ccgggggtgg ggccccctgg ttgaccgccc ggcccgccgt cctgtcctcc gggctgatgg
       61 tcgccctgac tgccggggtg accgccaggg gcgccggggt gcccaccctg ggcgccgggg
      121 tgccagggct gggagggatg cccgcccccc ggccccggag catgctggtg gccgttgtgc
      181 ttggcggcga gggcggcgac cggtacgacc agcagccccg tcgcgatcgc aagtgtcaag
      241 cgccctcgta ccctcatcgg ccctccttgc ctgtggcctc ggctccccca cttggaaagt
      301 ccgatcgtcc ggatcagtca cacccctaac gcgagccgag ggccggcatc acgcggcgct
      361 ggctagggtt gtcccgtgac cgcggcagcc gatcccccat ccgcagccac cgcaccgccg
      421 agcgtgtgga gcatcgcctc gaagatggtc ggcggggcgc tgcgctcgac cccggcgaag
      481 gtcatcgggg ccgtgatcgc ggccctggtc ctactaggcg cggtgctcag cgggatcgcg
      541 aacggcatcg gcggcttctt cggcacgctg ctggtcgcgc tgttcgtgct cggcgtggtc
      601 gggttcatcg tcgcggtccc ggtgttcgtc gtcgtcctgt tcatcgcctc gagccgggcc
      661 cgaagcgccg agtcgagcgc gatgcagcag tacgcgctga ctcggcggct cacctactcg
      721 ccgtcgggca cgctcctcga agcgacgccg ctgctgtcgg ccggaacctc gcgccagacc
      781 caggacgtga tgacgggcct gctgcccggc ggccttcagg ggacgctcgc ccactacacc
      841 tactacgtga ggcaccacac ccagcagggc agccaccgag tcccctatcc gaacaccgtc
      901 gtgatagccc agatccccga gagcacggca tacgtcccca agctgctgtg ccacgacggg
      961 cgccggatcg ggggagcgaa catcttcggg ctcgacctct cgggtgacgc cgaggtcgag
     1021 ctggagagca cggcgctcaa cgagcgctac agcatcagga cggcggccgg ccaggaccag
     1081 gcgtggcttc gcgagctgtt caccccggtg ttcatcgact ggctcgccgc caaggcggcc
     1141 gccgagttct ccttcgagct cgtcaacggc ctgctctgcg tctcgatcgc agggcgcctc
     1201 gaccgcgccg accagctcga ctggctctgc ggcgccgcca cctacgtagc gggacggatc
     1261 cgaggcgagg tcaccgagta gggcgcccgg ctatgcctgg accccgagcg cgacctttgc
     1321 ctcgtagccc ttcttcttct tgcccttctt gttacgcgcg cgcttgacgt tgagcaccaa
     1381 cgcgaaaggg gcggccatca tggccgccct ttcgctgggc cagtgctgcg cccgggttag
     1441 gagccgagta cgaccttctc gaactcgttc ccgttcgcgc tcacctcgac cgtcgcctcg
     1501 tggacgatcg tcccgggcac gaggtcgtcg gtgctgcact cggtgccctc cgggcagttg
     1561 tgctcgccgt cgccagacgg cagcgcgcca ccctcgggcg gctgctgcga ggtcccgctc
     1621 ggacccgtcc cctcggcggg cggctggggc gcaaacatcg cggtctgctc ctcatcgccc
     1681 tccggggcgc agatgatcct ggtctcggcg gtgacctcac cactaagggt gccgccggcg
     1741 tagaggtcga tcaccagcgt tccggtgccc tcctctgtgc cgggcgtgaa cgagctcacc
     1801 tcgccggcgt tctcgtcggc gtcggggaca ccgtcaccgt cgctgtcgtc ctcgcgcggg
     1861 ttggtgccgg cgtgatactc ggcgcgattg cgcatctggt cgccgtcctg gtcgcgcttt
     1921 gcctggttga ccttcagcga caggtcgtag tgcttctccc actggtccgg gatgtggtcg
     1981 gagttgacgt cccgcgccga cgccgacccg actaggccga tcatcagcaa tgcgacaccg
     2041 acgcacacgc cgatcgcgat gtgggccacc cggttcgcgt gaactcgatc ctgcgtcatg
     2101 gccttccttc cccggcgccg gcctgcgctc gccgtgagct gtggccgggc cgcttcgccg
     2161 tttgcgattg cggccctcta acgcggcccc gcggccgata tcacggccgg gccgcgaagg
     2221 caggaaaggc gccggctact ggaagtcgcc ggggccgccg gagcccggcg gaggctgggt
     2281 gccgctgtcg ggataggagg tcgaggtgta ggtcgagcct ccatccggcg gcggacag
//
LOCUS       DASPWO010000251         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_548039, whole genome shotgun sequence.
ACCESSION   DASPWO010000251 DASPWO010000000
VERSION     DASPWO010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548039"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2010
                     /locus_tag="VEO00_05595"
     CDS             <1..2010
                     /locus_tag="VEO00_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57506.1"
                     /translation="RIALCFALGIAVAGTIGFALALARSLYPATFFGLWAVATAGLLL
                     ASWRRAGARARLSDARDEVRGAPWTIGVGLAVFAAFAAVRLAVDPALHFGTSAAWRYW
                     ADALEIADAHRIPAEVLQYGQVFPAVVNKVFLNALNAGVAYGAGTGAPGALAGLGATL
                     WVGAVGSFAALWALGHELGLRLTAPLLPLLAFADRVVLNTETTTDLDTFKAEIGGRLL
                     AFAGAALLVRALRRSWERSGDPDAERVRGRRDAAVGGALIGLAAAVHAVPVLIVLPFL
                     AWYAAAGVARRRLARPVLGRAVRVAVAGGLVAAAVAAVLLVLPRGDLGLGLRGPGGNE
                     ASEFGPGFDPTRYLNAGTLPGEQEVGPDTWYIPPGRALREYTASALAVRAGSLPRLLP
                     PALFAAGLLVALAMAWRFPARLRPLGVAAWGLGATIVGLTWFFSARYDFYIPSFFGVR
                     RLFDYSSLPLALGGAAVLEGGLALLGRRRPRPTVIAGAVAVVAVAAVLLPTALPGRAG
                     GTRLAAFDEAFAWIRGHTPCDARLLADQHTEGAFEVNTGRVAILEGATPYLRPEILNA
                     IVRLHLLAREFFQLPPAGGRVLIGLRATHVIDVKAGGIGYSEPIGPVDRQELPDAGFL
                     RLVYESPVMDVYAVEAPEGAPGMPRQAAPREFPGYACPVTAQTGP"
     gene            complement(1988..>2588)
                     /locus_tag="VEO00_05600"
     CDS             complement(1988..>2588)
                     /locus_tag="VEO00_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57507.1"
                     /translation="QGIRERGRAELGGVDGLAMLKRTLAYQRPVTTLDTKLADGSLDL
                     DGIAGRIEENLFYGIYPGAWVRAENEDHQTEPTWSTEAKRAVFARFTPLFRRLAAAGW
                     EPVTDARTDDSGVWVERYGSAADGDLHLALRNHTGRERDVTLEVDLRAQPLGEESRVE
                     ELLTGAEVRPDRAGDVLFLTVSVPADSTRLLAFTDPSAR"
BASE COUNT          237 a          972 c          993 g          386 t
ORIGIN      
        1 cgcatcgcgc tgtgcttcgc gctcgggatc gccgtcgcgg gcacgatcgg gttcgcgctg
       61 gcgctggcgc ggtcgctgta cccggcgacg ttcttcgggc tgtgggccgt ggcgacggcg
      121 gggctgctgc tggcgtcctg gcgccgggcc ggcgcgcggg cgcgtctctc cgacgcgcgc
      181 gacgaggtcc gcggcgcgcc ctggacgatc ggggtcgggc tcgcggtgtt cgccgcgttc
      241 gccgccgtcc ggctggccgt cgaccccgcg ctgcacttcg gcacgagcgc cgcctggcgc
      301 tactgggccg acgcgctgga gatcgccgac gcccaccgca tcccggccga agtcctgcag
      361 tacggccagg tcttccccgc cgtggtgaac aaggtgttcc tgaacgcgct gaacgccggc
      421 gtggcgtacg gcgccgggac cggggcgccc ggggcgctgg ccgggctggg tgcgacgctg
      481 tgggtcggcg ctgtggggtc gttcgccgcg ctctgggcgc tcggccacga gctcgggctg
      541 cgcctgacgg ccccgctgct gccgctgctg gcgttcgccg accgggtcgt gctgaacacg
      601 gagacgacga cggacctgga cacgttcaag gccgagatcg gcggacggct cctggcgttc
      661 gccggcgcgg cgctcctggt ccgcgccctg cgccggtcgt gggagcggtc gggcgacccc
      721 gacgcggaac gcgtgcgggg gcgccgggac gccgccgtcg gcggggccct gatcggcctg
      781 gcggccgccg tgcacgccgt ccccgtgctg atcgtgctgc cgttcctggc ctggtacgcg
      841 gccgccggcg tggcccgccg ccgcctggcc cgcccggtcc tcggccgggc cgtgcgggtc
      901 gccgtcgccg gcggcctggt ggcggccgcc gtggccgccg tcctcctggt gctcccgcgg
      961 ggtgacctcg ggctcgggct gcgcgggccg ggcggcaacg aggcgtcgga gttcggcccc
     1021 gggttcgacc ccacccggta cctgaacgcc ggcacgcttc ccggtgagca ggaggtcggt
     1081 cccgacacgt ggtacatccc gcccgggcgg gcactccgcg agtacacggc gagcgcgctg
     1141 gccgtgcgcg ccggctcgct cccccggctg ctgccgccgg cactgttcgc ggcggggctg
     1201 ctcgtcgcgc tggcgatggc ctggcgcttc ccggcacggc tgcggccgct gggggtcgcg
     1261 gcctgggggc tcggcgcgac gatcgtcggg ctgacgtggt tcttctccgc gcgctacgac
     1321 ttctacatcc cgtccttctt cggcgtccgc cggctcttcg attactcctc cctgccgctc
     1381 gcgctcggcg gcgccgcggt gctcgagggc gggctggcgc tcctcggccg tcgccgaccg
     1441 cggccgacgg tgatcgccgg cgcggtcgcc gtggtggccg tcgccgcggt gctcctgccg
     1501 accgcgctgc ccggccgggc cggtggcacc cgcctggccg ccttcgatga ggcgttcgcg
     1561 tggatccgcg ggcacacccc ctgcgacgcg cggctgctgg ccgaccagca caccgagggc
     1621 gccttcgagg tgaataccgg gcgcgtggcg atcctggagg gggccacgcc gtacctgcgc
     1681 ccggagatcc tgaacgccat cgtccgcctg caccttctcg cccgcgagtt cttccagctg
     1741 cctccggccg gggggcgcgt cttgatcggc ctgcgcgcga cgcatgtcat cgacgtcaag
     1801 gccggcggga tcggctattc cgagccgatc ggcccggtcg accgccaaga gctgcctgac
     1861 gccgggttcc tgcggctcgt ctacgagagc ccggtcatgg atgtctatgc ggtcgaggcc
     1921 ccggagggcg cgccggggat gcctcggcag gcagcgcctc gggagttccc cggctacgcg
     1981 tgcccggtca ccgcgcagac gggtccgtga acgccagcag ccgggtcgag tcggcgggga
     2041 cggacacggt caggaagagc acgtcgccgg cgcggtcggg ccgtacctcg gcgccggtca
     2101 gcagctcctc gacgcgggac tcctccccga gcggctgcgc gcggaggtcg acctccagcg
     2161 tgacgtcgcg ctcccgcccg gtgtggttgc gcagcgccag gtggaggtcg ccgtccgcgg
     2221 ccgagccgta gcgctccacc cacaccccgg agtcgtcggt gcgggcgtcc gtgaccggct
     2281 cccagccggc cgccgccagg cggcggaaca gcggcgtgaa gcgggcgaac acggcacgct
     2341 tcgcctcggt gctccaggtc ggctcggtct ggtggtcctc gttctcggcg cggacccagg
     2401 cgccggggta gatcccgtag aagaggttct cctcgatccg cccggcgatc ccatcgaggt
     2461 cgaggctgcc gtcggcgagc ttcgtgtcga gcgtcgtcac cgggcgctgg taggcgagtg
     2521 tccgcttcag catcgccagg ccgtccacgc cgccgagctc ggccctcccc cgctcgcgga
     2581 tgccctgc
//
LOCUS       DASPWO010000252         6482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_37453, whole genome shotgun sequence.
ACCESSION   DASPWO010000252 DASPWO010000000
VERSION     DASPWO010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37453"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1562
                     /locus_tag="VEO00_05605"
     CDS             156..1562
                     /locus_tag="VEO00_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57508.1"
                     /translation="MTNVSRAIPAPYMSAGPVCTAGAAGAGTVGSGAVGAPASTGVGC
                     VAARSWPHAAQKRASDSFSVPQFQLPQDGGVGTAGAAVSASVTLAMALLLGTAILVWL
                     LYRAGRGIGRRAGGNGWVRGLHALGLALAYALVALAVSFAFRWRLSVPDTPFTRQGGT
                     ITIQPSPLPAFLWPFAIAAVAGFLGAFRSAPAPAESEARAADRWTRGAVSGGWRMAAI
                     GLVLSYAGFAVNAAVTPGLPREYFRSVVRTDASYGVVNAFLVPNIAGLVLPFAMGSTL
                     GLEGGGSSVTIASLTSFPEGVDLSALDPSAAVGGQIPANVLLSGALPPAYFLYLLVPL
                     AAVLAGGIAAARRVGPRSRAGGAAAGALAGVAFVPLAALIVVLAGVGMRVSLSFAGLA
                     TAQTARVGASFWSTILLAAAWGIGGGALGGLIARPAEAPSAPALAGSAEPPPPPPAEA
                     PPTEPTPTTTSPPEPPAE"
     gene            complement(1622..2098)
                     /locus_tag="VEO00_05610"
     CDS             complement(1622..2098)
                     /locus_tag="VEO00_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57509.1"
                     /translation="MATSPGFDMSRMSTASKILIGASALFFIDGFLPWQRVCIDVGIP
                     GIGGCASASLWSGIGFLAALLGLALLVWEGLRVAGVNLGTMPEATKNMISAGLAAGVV
                     LFTALKVLIDNEAIAYGAWIGIILALAIGYGGFMRWQESKTATPMPPAAPPPPPPA"
     gene            complement(2181..2642)
                     /locus_tag="VEO00_05615"
     CDS             complement(2181..2642)
                     /locus_tag="VEO00_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57510.1"
                     /translation="MGQECLRRVLPDPPPLPDPGPAGPSLADRVAATAFAVAAMVSLF
                     AWSRFGAGAGGFTPWGLSPPRWAWLAGAPAVIGLLLASVGARARSRLPGALRGWLAVL
                     FGGIVLSGAVLFVWWPPFATRANFVPWIAAAAATTAVGAGVALLRAARLVA"
     gene            complement(2782..3804)
                     /locus_tag="VEO00_05620"
     CDS             complement(2782..3804)
                     /locus_tag="VEO00_05620"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HYU57511.1"
                     /translation="MTRCPWCAEEIPPEEAGARFCPFCGSDLTQAPPSPPAVAVPQFT
                     HSGNRFVLGHGPDFYGIWDRTSPAEPAQRFPRTDQGWAEAWRWFSSWEPSAAPVNPDG
                     SLGPPIGPLFQPTDALSRWVIAGLVAVPLLNLAQIPHNLRQMRLLSDTIAGRISIREL
                     NARLAGPGVIGGLADLVGLTLIVLWLVWQHRSQSNLRALGMQDLRYTPGWAVGWWFIP
                     VANIWMPYLTVRELWRASDPAAGPSDWKTRQARPVLAWWLTYLLPGVVIVPLFVIAAV
                     QAADTEDVLPLLRVLQAMTIGGAVLQVAAAALATVIVLTVTRNQVAKRAVSVAAPTAN
                     LPGADG"
     gene            complement(3844..4188)
                     /locus_tag="VEO00_05625"
     CDS             complement(3844..4188)
                     /locus_tag="VEO00_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57512.1"
                     /translation="MRPPLVTAAAVLLIIGGALGILGGLLLFGLSGIGGFFTLFGIIG
                     IGVGALELYAGVQVLALKRIGWTLGIALAAISAVVSIVLVTKTAGTNIIGILIDAFII
                     YALYTNSSHFRN"
     gene            4376..5014
                     /gene="purN"
                     /locus_tag="VEO00_05630"
     CDS             4376..5014
                     /gene="purN"
                     /locus_tag="VEO00_05630"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006694314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HYU57513.1"
                     /translation="MDGRVAVLASGAGSNLGALLSDPEVGPRIVLVLTDRPGAGALER
                     AREAGVATETVDREAFPDRAAFDRGLLERLRAHAVDALVLAGYMRILGPEVVRAFPDR
                     ILNVHPSLLPAFPGPHAVREALAWGVRVTGCTVHLVDEEVDHGPIVFQEAVPVVPGDD
                     EASLHARIKQAEHRLLPAAARLLVEGRLAVEDRMVRVLPVPDGAPLGERVGP"
     gene            5011..>6482
                     /gene="purH"
                     /locus_tag="VEO00_05635"
     CDS             5011..>6482
                     /gene="purH"
                     /locus_tag="VEO00_05635"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HYU57514.1"
                     /translation="MIDVLPIRRALVSVADKAGLIPFAQALLRHGVEIVSSGATAALL
                     VDAGVHAVPVADVTGFPEMLGGRVKTLHPRVHAGILADKDEPEHLRQLAEQGIAPFDL
                     VVANLYPFREAVRAGARPEEVIEQIDVGGPAMVRAAAKNFGSVAVVVSPQRYLAIAEE
                     IRDQGGLSRETRAALAREAFAHTAAYDAAIASWFGREGEGLPATIGAAYLKLGDLRYG
                     ENPHQRGALYRLEDGPGPLGGAEVLQGKEMSFNNWLDSEAAWALARTLRGPAAAIVKH
                     HNPCGAAEAADLASAYRAALASDPVSAYGGVVAFAGPPGGAAAAAMAGVFTEVVVAPD
                     FTPEALAAFVDRPNLRVVRVPVHEPAELELRPIDGGALVQEADRVEEGREEMKVVSTR
                     APTEAEWTDLLFAWRVAARVKSNAIVLAAGRATVGIGAGQMSRVDSVDLARAKAGERA
                     AGSAMASDAFFPFRDGIDHAAAAGVAAVIHPGGSVRDEEVV"
BASE COUNT          867 a         2297 c         2502 g          816 t
ORIGIN      
        1 gcggcagctg gaactgcggg atcgtgacgc gcacgcccac gtgatggaac gagtacgtga
       61 acaggccgcc gacccgcacg acgtcggtcg cgccgaggtc gccggggttc agcgcgtaga
      121 tcaggaacgc cagcgcctcc ccgaggagca gtgcgatgac gaacgtcagc agggccatcc
      181 cggcgccgta catgagcgcc ggtcccgtct gcaccgccgg cgcggccgga gcgggaaccg
      241 tcggcagcgg ggcggtcggc gcgccggcat cgacgggcgt cggctgcgtg gcagccaggt
      301 cgtggccgca cgcggcacag aagcgggcgt cggactcgtt ctcggtcccg cagttccagc
      361 tgccgcagga cgggggcgtc ggcaccgccg gcgccgcggt ctcggcgtcg gtgacgctgg
      421 cgatggcgct gctgctcggg acggcgatcc tggtctggct gctgtaccgg gcaggccgcg
      481 ggatcggccg gcgcgcgggc gggaacgggt gggtccgtgg cctgcacgcg ctgggcctgg
      541 cgctggccta cgcgctggtg gcgctcgccg tctcgttcgc gttccggtgg aggctctcgg
      601 tgccggacac gccgttcacg cggcagggcg ggacgataac gatccagccg tcccccctcc
      661 ccgcgttcct ctggccgttc gccatcgccg ccgtggcggg gttcctgggc gccttccgga
      721 gcgcgccggc cccggcggag agcgaggcgc gcgcggcgga ccgctggacg cgcggggcgg
      781 tgagcggtgg atggcggatg gccgccatcg ggctggtgct gtcctacgcc gggttcgcgg
      841 tgaacgcggc ggtgacgccg gggctgccac gcgagtactt ccggagcgtc gtccggaccg
      901 acgcgtccta cggggtggtg aacgctttcc tggtgccgaa tatcgccggc ctggttctcc
      961 cgttcgcgat gggctccacg ctgggcttgg aggggggcgg ctcgagcgtg acgatcgcgt
     1021 cactgaccag cttcccggaa ggcgtggatc tctccgcgct cgacccgagc gccgccgtcg
     1081 ggggacagat cccggcaaac gtgctcctat cgggggcgct cccgcccgcg tacttcctgt
     1141 acctcctcgt cccgctcgct gcggtcctcg ccggcgggat cgccgccgcc cggcgcgttg
     1201 ggccccgttc ccgcgccggg ggagcagcgg ccggcgcgct ggcgggcgtc gccttcgtcc
     1261 ccctcgcggc tctgatcgtg gtcctcgccg gcgtgggcat gcgcgtctcg ctctcgttcg
     1321 ccgggctggc caccgctcag acggcccggg tcggggcctc gttctggagc acgatcctgc
     1381 tggcggcggc ctgggggatc gggggcgggg cgctcggcgg gctcatcgct cgtccggccg
     1441 aggcgccgtc ggcgccggcc ctcgccggct ccgccgagcc gccgccgcca ccacccgccg
     1501 aggcgccacc aaccgagccg acgccgacca cgacgagtcc accggagccg ccggccgagt
     1561 gaagccgccc aacgaacggg cccgggcgat gagcccgggc ccgtcggtgc tgccgggtcg
     1621 atcaggcagg cggcggaggc ggtggggcgg ccggcggcat gggggtcgcc gtcttcgact
     1681 cctgccaccg catgaacccg ccgtagccga tggcgagcgc caggatgatc ccgatccagg
     1741 cgccgtacgc gatggcttcg ttgtcgatca ggaccttgag ggccgtgaac aggaccaccc
     1801 cggcggccag gcccgcgctg atcatgttct tggtcgcctc gggcatcgtg cccaggttca
     1861 cgcccgcgac ccgcagcccc tcccacacca ggagggccag gcccagcagg gcggcgagga
     1921 acccgatgcc ggaccagaga gaggctgagg cacacccacc gatcccgggg attcccacgt
     1981 cgatgcaaac ccgctgccag ggcaggaacc cgtcgatgaa gaacagggca ctggcgccga
     2041 tcaggatctt gctcgcggtc gacatgcgcg acatgtcgaa ccccggactg gtcgccatac
     2101 ggtctccttt cggaacgctt cccctgggac ggccgaacgg cccgcccgag cgccgattgt
     2161 tccgcctggg gtatgcggag tcaagcgacc agccgagcag cccggagcag ggccacaccg
     2221 gccccgacgg ccgtggtggc cgccgcggcg gcgatccagg ggacgaagtt cgcccgggta
     2281 gcgaacggcg gccaccacac gaacaggacc gcaccggaga ggacgatccc gccgaacagg
     2341 acggccagcc agccgcggag ggcgccgggc aggcggctcc gcgcccgggc ccccacggag
     2401 gcgagcagga gcccgatcac ggccggggcg ccggccagcc aggcccaccg cggcggggag
     2461 aggccccagg gagtgaagcc gcccgcgccg gcgccgaagc ggctccaggc gaacagcgac
     2521 accatcgcgg ccaccgcgaa ggccgtggcg gcaacccggt cggcgaggct cgggccggca
     2581 gggccggggt cggggagcgg cggcgggtcg gggagcacgc ggcgcaggca ctcctggccc
     2641 acgagctcgc cgcgtaccgg gatcgcacat tcgaggcaca ccggccgccc gcacgccccg
     2701 caggccccga tggccgcacg gccggggtgg gtgtgacacc gggaccgctg agcgaccgtc
     2761 ggctccatcc gacggccgag tctacccgtc cgctccgggg aggttcgccg tcggggcggc
     2821 cacggagacc gcgcgcttcg cgacctggtt ccgggtcacc gtcaggacga tcacggtcgc
     2881 cagggcagcc gccgcgacct gcaggaccgc ccctccgatc gtcatcgcct gcagcacgcg
     2941 aaggaggggc agcacgtctt cagtgtccgc cgcctgaacg gcggcgatta cgaagagcgg
     3001 cacgatcacc acgcccggca ggaggtaggt cagccaccag gccaggacgg gtcgcgcctg
     3061 acgggtcttc cagtcgctcg ggccggcggc cgggtcgctc gcccgccaca gctcgcgaac
     3121 cgtcaggtat ggcatccaga tgttcgccac tgggatgaac caccacccga ccgcccaccc
     3181 cggggtgtag cgcaggtcct gcatccccag tgcgcggagg ttggactgac tgcgatgctg
     3241 ccagacgagc cagagaacga tgagggtcag gccgaccagg tccgccagcc ccccgatgac
     3301 gccgggaccg gccaggcgcg cgttcagctc ccggatcgag atcctgccgg cgatggtgtc
     3361 cgagaggagc cgcatctgac ggaggttgtg ggggatctgc gccaggttga ggagcggaac
     3421 cgcgacgagg cccgcgatga cccatcgcga caaagcgtcg gtgggctgga agagcgggcc
     3481 gatcggcggt ccaaggctcc cgtccgggtt gaccggggcg gcggagggtt cccaggagga
     3541 gaaccagcgc caggcctcgg cccagccctg gtcggtgcgg gggaagcgct gggccggctc
     3601 ggcggggctg gtgcgatccc agatcccgta gaagtccggg ccgtgcccca gcacgaagcg
     3661 gttcccggag tgggtgaact ggggaacggc cacggcgggc ggcgatggcg gggcctgggt
     3721 caggtcgctc ccgcagaagg ggcagaagcg agcgcccgcc tcctcggggg ggatctcctc
     3781 cgcgcaccag ggacagcgcg tcatcgttcg tgcgggggcg gccgaagccg ccccgcgaag
     3841 ccctcagttc cggaagtgag aggagttcgt gtacagcgcg tagatgatga acgcgtcgat
     3901 caggatcccg atgatgttgg tcccggccgt cttcgtgacc agcacgatcg agacgacggc
     3961 ggatatggcg gccagcgcga tcccgagcgt ccagccgatc cgcttcagcg cgagaacctg
     4021 aacgccggca tacagctcga gggctccgac accgatcccg atgatcccga acagggtgaa
     4081 gaaccctccg atcccggaca ggccgaacag caacagcccg ccgaggatcc ccagcgcccc
     4141 gccgatgatc agcagcacgg cggcggcggt caccagtggc ggtcgcatcg cggacccacc
     4201 ggaggcgggc ggaggcattt gggacatacg cgctcccttc tcgagcgtcg gacagggcgt
     4261 cggcgcagtc taggggttgg ggggaggggc gcacaagggc gctccgaatg ccccggcctc
     4321 cgcgagccag gggttcgtat tgacagtgcg gcggggcgcc cataggttcc gcgcggtgga
     4381 cggtcgggtg gcggtgctcg cctcgggggc cgggtccaac ctcggggcgc tcctgtccga
     4441 cccggaggtc ggcccgcgga tcgtgctcgt cctgaccgac cggcccggcg ccggcgcgct
     4501 ggagcgggcc cgcgaggccg gggtcgcaac agagaccgtg gaccgcgagg ccttccccga
     4561 ccgggccgcc ttcgaccgcg ggctgctgga gcggctgcgg gcccatgcgg tggacgccct
     4621 ggtgctcgcc ggctacatgc gcatcctcgg cccggaggtc gtgcgcgcct tcccggaccg
     4681 catcctgaac gtccacccct cgctgctgcc cgccttcccc gggccgcacg ccgtccggga
     4741 agccctggcc tggggggtgc gcgtcaccgg ctgcacggtc cacctggtgg acgaggaggt
     4801 ggaccacggc ccgatcgtct tccaggaggc ggtgccggtg gtccccggcg acgacgaggc
     4861 ctcgctgcac gcccggatca agcaggccga gcaccggctg ctgccggcgg cggcccgcct
     4921 gctggtggag gggcggctcg cggtcgagga ccggatggtg cgggtcctcc ccgtgccgga
     4981 tggagcgccg ctcggggagc gggtcggccc gtgatcgacg tcctgccgat ccgccgcgcg
     5041 ctcgtctccg tcgcggacaa ggccgggctg atcccgttcg cccaggcgct gctgcgccac
     5101 ggggtcgaga tcgtctcgtc gggggcgacg gcggcgctcc tggtcgacgc cggcgtccat
     5161 gctgtgccgg tggccgacgt caccgggttc ccggagatgc tcggcggccg ggtgaagacg
     5221 ctccacccgc gcgtccacgc cggcatcctg gccgacaagg acgagcccga gcaccttcgg
     5281 cagctggccg agcagggcat cgcgccgttc gacctggtgg tggccaacct ctatccgttc
     5341 cgtgaggccg tccgcgccgg tgcccgcccg gaggaggtca tcgagcagat cgacgtcggc
     5401 gggccggcga tggtccgggc cgcggcgaag aacttcgggt cggtcgccgt ggtggtctcg
     5461 ccgcagcgct acctggcgat cgccgaggag atccgcgacc agggcgggct gtcccgcgag
     5521 acgcgggcgg cgctggcgcg ggaggcgttc gcgcacaccg ccgcctacga cgccgcgatc
     5581 gcctcgtggt tcggtcgcga aggggagggc ctgccggcga cgatcggcgc ggcctacctc
     5641 aagctgggcg acctgcgcta cggggagaac ccccaccagc gcggcgccct gtaccggctg
     5701 gaggacggac ccgggccgct cggcggcgcc gaggtgctgc aggggaagga gatgtcgttc
     5761 aacaactggc tggactccga ggcggcctgg gcgctggcgc gcacgctccg gggaccggcc
     5821 gcggccatcg tcaagcacca caacccctgc ggggcggccg aggccgccga cctcgcgtcg
     5881 gcgtatcgcg ccgcgctggc ctccgacccc gtctcggcct acggaggcgt ggtggcgttc
     5941 gccggcccgc cgggcggcgc cgccgccgcg gcgatggccg gggtcttcac cgaggtcgtg
     6001 gtggcgcccg acttcacccc cgaggcgctg gcggcgttcg tcgatcggcc gaacctccgc
     6061 gtggtgcggg tgccggtcca cgagccggcc gagctcgagc tgcgcccgat cgacggcgga
     6121 gcgctggtcc aggaggccga ccgggtggag gaagggcgcg aggagatgaa ggtggtctcc
     6181 acgcgcgccc cgaccgaggc ggagtggacc gacctgctgt tcgcgtggcg ggtggcggcg
     6241 cgagtgaagt cgaacgcgat cgtgctggcg gcggggcggg cgacggtcgg gatcggcgcc
     6301 ggtcagatga gccgggtgga ctcggtggac ctcgcccggg ccaaggccgg ggagcgggcg
     6361 gccgggtcgg ccatggccag cgacgcgttc ttcccgttcc gcgacgggat cgaccatgcg
     6421 gccgcggcgg gcgtcgcggc ggtgatccac ccgggcggct cggtccgcga cgaggaggtc
     6481 gt
//
LOCUS       DASPWO010000253         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_459665, whole genome shotgun sequence.
ACCESSION   DASPWO010000253 DASPWO010000000
VERSION     DASPWO010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459665"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VEO00_05640"
     CDS             complement(<1..803)
                     /locus_tag="VEO00_05640"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYU57515.1"
                     /translation="MTMQPASLRALTPSFADLGASHPVIRALQRRGIDEPFPIQSLVL
                     PDAIAGRDVLAKSRTGSGKTLAFAVPIVERLRPSDRAPSALILVPTRELAVQVAGEFE
                     EVARARNLNVAVAYGGVSIREQSGRVGRAHILVATPGRLDDLARRKLIALDRIRILVL
                     DEADRMLDMGFQPQVDAIVRRLPKERQTMFFSATLDGSVGLLARAYTNGPAHHEVASP
                     RQTVDEVEHRFVPVRPEAKVEALAAMLREHQEPTLVFVRTRRGVSRLVTR"
     gene            complement(1076..1606)
                     /locus_tag="VEO00_05645"
     CDS             complement(1076..1606)
                     /locus_tag="VEO00_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYU57516.1"
                     /translation="MRRALALVLLAALVVAGGIVTGVALLAEEDTAAEARADRLAAVS
                     IFASLPRDDLVALAEVTDAVTFGDGEVIFREGDPGGTLYVIDAGAVRISTGSTTLATL
                     GAGEVFGELSLFGSDTRSADATADSAVTLLAIAHDDFTPFLESRPTVGIALLDTLADR
                     LRRTNADLAACQAGDA"
     gene            complement(1599..1976)
                     /locus_tag="VEO00_05650"
     CDS             complement(1599..1976)
                     /locus_tag="VEO00_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57517.1"
                     /translation="MDRKYRMYERRFLNRRGFYSSAFVLASVEDTSAGEGSEQHTPEV
                     ELRIGNCNRSISLEFDLYGADDRRNSLEKIDHLVFVLSAFRDALREEADLRDARQARR
                     ACSAPGEGATVPGDGSSEGGNDA"
BASE COUNT          327 a          776 c          860 g          307 t
ORIGIN      
        1 cgggtcacca gccgcgacac gccgcggcgc gtgcgcacga agacgagcgt cggctcctgg
       61 tgctcacgca gcatcgccgc cagcgcctcc accttcgcct ccgggcggac cgggacgaag
      121 cggtgctcga cctcgtcgac ggtctggcgg ggcgaggcca cctcgtggtg cgccgggccg
      181 ttcgtgtacg cgcgagccag cagcccgacc gacccgtcga gcgtggcgga gaagaacatc
      241 gtctggcgtt ccttcggcag gcggcggacg atggcgtcga cctgcggctg gaaccccatg
      301 tcgagcatcc ggtccgcctc gtcgaggacc aggatccgga tgcggtccag ggcgatcagc
      361 ttgcggcggg ccaggtcgtc gaggcgcccg ggcgtggcga ccaggatgtg ggcccggccg
      421 acccgcccgg actgctcgcg gatcgagacg ccgccgtagg ccacggcgac gttcaggttc
      481 cgggcgcggg cgacctcctc gaactccccg gcgacctgca ccgccagctc tcgggtcggc
      541 accaggatca gggcggaggg ggcgcggtcc gacggccgga gccgctcgac gatcgggacg
      601 gcgaaggcca gggtcttgcc cgaccccgtg cgggacttgg ccagcacgtc gcgcccggcg
      661 atggcgtcgg ggaggaccag ggactggatc gggaacggct cgtcgatgcc ccggcgctgc
      721 aacgcccgga tcacggggtg cgaggcgccg aggtcggcga agctgggcgt gagcgcgcgc
      781 aaagacgcgg gctgcatggt catggtgagg ctccttccgc ggcgccgggc gccgcacggt
      841 tcatcgggga ggcttcgtct tcgacccgat gccccggaaa ggaaccagag agaaacgaac
      901 cctcacggga cggccgagcg gccgtccgtg cgccgaggat agcagctcga ggcgcttctg
      961 accgtgggtc agccggaccg aactcgaagt gctatgggat ccgcaggtcc tcgatctgaa
     1021 cggacgccgg tgcgagtcgc gtcgatcgcc gatcctggtg ggacgcgcac caccgctagg
     1081 cgtcaccggc ctggcaggcg gccaggtcgg cgttggtccg gcgcaggcgg tcggccagcg
     1141 tgtcgagcag cgcgatgccg accgtcggcc gcgactcgag gaacggcgtg aagtcgtcgt
     1201 gcgcgatcgc gagcagggtg accgcggagt ccgccgtggc gtcggcggag cgggtgtcgg
     1261 acccgaagag cgagagctca ccgaacacct cgccggcccc gagcgtggcc agcgtggtcg
     1321 agccggtcga gatgcggacc gcgccggcgt cgatcacgta gagggtgccg ccgggatcgc
     1381 cctctcggaa gatcacctcg ccgtcgccaa acgtcaccgc gtcggtcacc tccgccagcg
     1441 cgacgaggtc atcgcggggg agggacgcga agatcgagac ggcggcgagc cggtcggcgc
     1501 gcgcctcggc cgccgtgtcc tcctcggcga gaagcgcgac gccggtgacg atgccccccg
     1561 ccaccacgag cgccgccagc agcaccagcg ccagagccct acgcatcgtt ccccccttcg
     1621 ctcgatccgt cgccgggaac cgtcgcacct tcgccggggg cggaacaagc gcggcgcgcc
     1681 tggcgggcgt cacggagatc cgcctcctcg cgcagcgcgt cgcggaaggc gctcaggacg
     1741 aagaccaggt ggtcgatctt ctccaggctg ttgcggcggt cgtcggcgcc gtagaggtcg
     1801 aactcgaggg agatggaacg gttgcagttg ccgatgcgga gctcgacctc gggtgtgtgc
     1861 tgctcggacc cctcgccggc ggaggtgtcc tcgacggagg cgaggacgaa cgcgctgctg
     1921 tagaacccgc gccggttcag gaaccggcgc tcgtacatgc ggtacttcct gtccacggtg
     1981 gcctcctcgg gcctcgggtc gctagcgcat gtccgtcgcc tcctctcggt cgatcatcgg
     2041 gattcgccgc cgccgcacgg ccggttcgct cgctcggata gggttgccgg cgagatggga
     2101 cggacgctga tcctcggcgg agggttcggc ggcgtgtcga ccgccgtgga gctccggcgg
     2161 atcgcccccg agcacgcggt gctgctcgtc gatcggggga cccatttcat gatgggcttc
     2221 cgcaagaacg acgaggtcgt cgggcgggct ccgatggccg aggggcgacg
//
LOCUS       DASPWO010000254        10797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_630285, whole genome shotgun sequence.
ACCESSION   DASPWO010000254 DASPWO010000000
VERSION     DASPWO010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10797
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630285"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VEO00_05655"
     CDS             <1..483
                     /locus_tag="VEO00_05655"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HYU57518.1"
                     /translation="RGLTDHTWAVYASDHGRLWGEHGLARKLFAYEESIRFPLLLWAP
                     GFPRTTIDQIVANVDVVPTLTALALDLEDHRYDGRSLLPLLAGDAADWRDALLIQNWA
                     TYRYDAVRTPRYTYVHWPRTGHEELYDLRRDPYELQSVAATRPAELERMRALLGRLLH
                     "
     gene            567..2387
                     /locus_tag="VEO00_05660"
     CDS             567..2387
                     /locus_tag="VEO00_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HYU57519.1"
                     /translation="MAIADETLCTMFWARVAERGEGPALRFRQGDGWGTITWRGYGEA
                     VRETASGLAALGVEPRQTVAVLSRNRPEWHVADVAGFTAGAITVPVYVTNSPPQVAYI
                     LGHSEARVAFVEDEEQLLKIEKERDGLPDLAHVVILDGVPSADGFVLTLDDLRERGRA
                     HDAAHPGAYEERWRAVRPEDTATIVYTSGTTGPPKGAMLSHHNAVWTVDSLLQVVQEP
                     PNAGRRLSFLPLSHIAERVTSHLSQIYNGLEVYFAESLDTVLRDLQEARPTVFFAVPR
                     VWEKFYAGIKAKLAQLPDEQRQMAEGAITVATAVVEMEQEGQEVVEEMRHGLEAAEGM
                     LFAPLRAQLGLDQAQDLISGAAPINEDILKFFHAVGLRIQEVYGQTEDTGPTSLNPKE
                     RIKIGTVGPPIPGLEVRIADDGEILVRGENVFQGYFKDPEATAEMLEDGWMRSGDVGE
                     LDEDGYLRITDRKKDLIITAAGKNIAPQELENLLKYHPLVSQAVVIGDRRPFLTALIT
                     LDAEALAKFAAERGLGDVPAGELAGREEVRQAIAGAVDAVNAEFSRAEQIKRWHVLPR
                     DFLQEEDEITPTLKVRRRAIIEKYGETIESLYAAGWEGSG"
     gene            complement(2384..3481)
                     /locus_tag="VEO00_05665"
     CDS             complement(2384..3481)
                     /locus_tag="VEO00_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57520.1"
                     /translation="MSRAGVRTVRTILATALVATVVTATGPPAGAVTPWPGPPPHQVL
                     PNDQADPKCPAGHHCTGVYVRCNWSGTWRTLRAWYAEDDRNPATPRGMVVFLSGGEGT
                     GYNWSNTPQSVPFRTALINAGLAIVHVRWYAAWNIAPSGDRAGMANVACRPVALLDRI
                     YTQEYVPLNITTPTGECGFCVVGNSDGADALAYALTHYGPDTTLKLAIDAVVPVSGPQ
                     QAAMRAGCLNQAGYQWDASHLIDIDESYGYRYTVGDPNPPPCYRHDTSPAWKDGRWVQ
                     DGVESGGTDYDYPETRIHLILGTVDKTTIRNHARAWETVLEAAAPALCDFQSTTTGSR
                     CREEFVAAMDVKIRSDPEGLNAVRLALVWTA"
     gene            complement(3484..4494)
                     /locus_tag="VEO00_05670"
     CDS             complement(3484..4494)
                     /locus_tag="VEO00_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57521.1"
                     /translation="MIQRAFLAAALIAAVLAVPATADTYPRPFGTFTALAGTDTTDCV
                     KIPGHTCKVYKVTGCPGLIGGRKLFIGTRRPPTGVATRGVVVTFSGGSGKRYWGAEGP
                     YSKAWIDNVVNAGMISAQVRWNGKWFQSNSGELAGPGAVTCRPATVVDWLYTDLYQPL
                     VAAGLNPADGRCGFCVVGSSMGSTVPVYLIEHFGMDSWIDGVFPTGGPIHAELARGCR
                     GEQGFNYGAEAEEWVDPTYGYVGGGGPCETHNLTDAWSARWTEDGLDTAGDHFHPTTR
                     VVIFVGELDPKLHKQAQFWEATLEAAGTPEVTLDIVPGMKHSIKGSQQGMNELQDAVL
                     LT"
     gene            complement(4564..5589)
                     /locus_tag="VEO00_05675"
     CDS             complement(4564..5589)
                     /locus_tag="VEO00_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57522.1"
                     /translation="MSARRRPAGAATVVAALLLATLGVALGVALAAPPAAAAPSPTGT
                     FRLNGRLDSTCPRGHSCRGFDVSCPGISRVARGFLAEPAKPRSPRGLVALFPGGSGKT
                     WWLGDGPNAGPFLADLRDAGLHAIQIRWTVKWSMAPAGERIGLARLACRPATVVRYLR
                     QTLYPQLGAPRHAAGVCGFCVVGNSWGSAQAAYPLAYYGLDGMLDMVALASGPTSTML
                     AAACEGDPDYRIEAHTAEADATYGFTGSDGPCARENASWHDRWVADGVATGGKDYHHP
                     ATRVHVILGGSDKPAVHNHARAYEARLRAAGTAEVTEEVIPGMGHSIKASAEGMKALL
                     EALLRVG"
     gene            complement(5586..6635)
                     /locus_tag="VEO00_05680"
     CDS             complement(5586..6635)
                     /locus_tag="VEO00_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57523.1"
                     /translation="MIEPVSPAPGRRARRAAQLAVFALAVAALPATPAAAGPLPLGTL
                     ATTAAVDKSCPSGYGCQGIEVHCPAVRKDARGFVATGPRPAAPRGLVVFLNAGSGKRY
                     WTETHDTARALVAELVDRGLRVVMVRWQTPWAVALSGERAGASALGCRPATALSWIRA
                     HRYPVPGMAPNEKVGRCGFCVVGSSSGTNQAAYPLSHYGLDASIDAAVLTSGPTLGAL
                     EKGCLDTPGYRYQGSYETDVDRAYGYIDSAGPCRRSEPAYQGIDWPARWRADGVATGG
                     SDYRHDTTRVHIIVGENDVNIPNHAADYRDRLLAAGSPQVCFQVIPDMGHGIKGSPEG
                     MRALRDALLGTACPA"
     gene            complement(6642..7844)
                     /locus_tag="VEO00_05685"
     CDS             complement(6642..7844)
                     /locus_tag="VEO00_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57524.1"
                     /translation="MRAAPRRRAIAVAMVALLIGLSGALTPGPAAATTDPVPTLQPLE
                     ELLPEIKYADSKCPVGYKCKAYKAVCDWGAGVRKTKLWIAENKNNPGYVGGTREFTVE
                     PRGVAVFFSGGSGKTYWYPEKNTSNQYYVNKMMDAGIYVVDLRWNGTWNAAPSGDRSA
                     GFDDLACRSSEVVKHVYQNIYVPKVLTPGNAPTTPFECGFCVSGYSAGTAEAVYSISH
                     YTPQLAAVNAVPDAVVIASGPIYTALDLACQGKGVGEFKMAPDHYVDVDESYGYIGVQ
                     GPCANTNPAGVNWPPKWRNDGIANTSAYGATPYGAPQGTYNHPDTRIEVLLGAVDNHP
                     VWHHSEVWQGVVEQYTAGCDFSAGVLGSHCFEYFVPYMSHSIKESPLGMLAMTRAITW
                     PTAVPPPS"
     gene            complement(7956..9086)
                     /locus_tag="VEO00_05690"
     CDS             complement(7956..9086)
                     /locus_tag="VEO00_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57525.1"
                     /translation="MPLVPRALLPLLVLALLSPATGRAAVPAATEAVATPAQMQLVPL
                     TGSLANDTKCPTGSRCNGTGVIVKCNWNGTWRNSKAWFAERNDGLTSPRGVVVFLSGG
                     NGKGYTWGTGDASPQMFTDLKNHNIHVVQARWNGNWMLGPSGDVSAGFAHLGCRPYGL
                     ITWVYQKVYQQEFHLDQVPHPQFECGFCVAGTSYGTEQVAYPMTVYGVAGDGTGANAF
                     VDAAFFAAGPTLAGMVKACTKVYGYYYDKTHRADVDKSYGDTSGTGPCVSYSTLAIWR
                     QTRWPNDGVDTGAIYTAFPNTRVHIILGKLDKESVWKHARDWEGQLENASPGDCDFQT
                     SGGDVIGEPCYEILIPGMGHAIRDSLAGLANLTRGVIWPETL"
     gene            complement(9131..10258)
                     /locus_tag="VEO00_05695"
     CDS             complement(9131..10258)
                     /locus_tag="VEO00_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57526.1"
                     /translation="MIRTSPSIRRAVLSALAAGLALAACSPKPVPIGPQRTSPRGGHG
                     GSPTGSPGTLPSGSGPPTVIPRAEPLPLGDLTEEGGSDSMCPPGFTCHAFTVDCPGVE
                     RADPGFYAVSEIPDGAAGMAVFFNAGPGKRYWGEEQAPQDFLGSVVDAGLVVVEVRWQ
                     IPWQLAEPGEDAGPAALACRPATAIRFMHDTLYPDLGLTPEAGAGACGFCLVGSSSGA
                     SQVAYALTHYGLQDLVDAAVLTSGPVHGALAAGCLIGGGEGYGAYARVIDRAYGYANG
                     EGPCRAHDPSWEERWLEDGVATGGAAYDYPDTRVQFIAGERDHGVQGLAHPYFERLVA
                     SGTPEVGFAVIPGMGHGIKGSAEGLDSLLRALTTARVDNPG"
     gene            10352..10759
                     /locus_tag="VEO00_05700"
     CDS             10352..10759
                     /locus_tag="VEO00_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57527.1"
                     /translation="MRSRHVSTVDQGFVPATAAVCYGRLADPAGYPAWWPGAAVRDAP
                     GGTAIEIGGLRMEGVRPDGVRPGVGLYLRFNGRGGAGSLEWYLEPFDGGTIVSAIAEL
                     DPRRPWGRRRVLRFRAAIRDGLVGLRAFAEANA"
BASE COUNT         1474 a         3773 c         4014 g         1536 t
ORIGIN      
        1 cgcgggctga ccgaccacac ctgggcggtg tacgcctccg accacggccg gctgtggggc
       61 gagcacggcc tggcgcggaa gctgttcgcc tacgaggaga gcatccgctt cccgctgctg
      121 ctgtgggcgc caggcttccc gcggaccacg atcgaccaga tcgtggcgaa cgtggacgtg
      181 gtgccgaccc tcaccgcgct cgccctggac ctcgaggacc accgctacga cggccgcagc
      241 ctgctgcccc tgctcgccgg cgacgcggca gactggcggg acgccttgct gatccagaac
      301 tgggcgacct accgctacga cgcggtgcgc acgccgcgct acacgtacgt gcactggccg
      361 cgtaccggcc acgaggagct gtacgacctg cgccgcgacc cctacgagct gcagagcgtg
      421 gcggcgacgc gcccggccga gctcgagcgg atgcgcgcgc tgctcgggcg gctgctccac
      481 tgagggcgac gcccgccggg tggcgccgac ggccgcggat cggcacgata gggatctcgg
      541 cgagcctggg tgaggggaga ggaacgatgg cgatcgcgga cgagacgctc tgcacgatgt
      601 tctgggccag ggtcgcggag cggggcgaag gcccggcgct gcgcttccgg cagggcgacg
      661 gctgggggac gatcacctgg cggggctacg gcgaggcggt ccgcgagacg gcgtccgggc
      721 tcgccgcgct cggcgtcgag ccgcgccaga cggtggccgt cctgtcgcgg aaccggcccg
      781 agtggcacgt cgccgacgtc gccgggttca cggccggcgc gatcaccgtc ccggtttacg
      841 tgaccaactc gccgccgcag gtggcctaca tcctgggcca ctccgaggcc cgcgtggcct
      901 tcgtcgagga cgaggagcag ctgctgaaga tcgagaagga gcgcgacggg ctgccggatc
      961 tcgcacacgt ggtgatcctc gacggcgtcc cctcggccga cgggttcgtg ctgacgctcg
     1021 acgacctgcg cgaacgcggc cgggcgcacg acgcggcgca ccccggcgcg tacgaggagc
     1081 gctggcgggc cgtgcggccc gaggacacgg cgacgatcgt ctacacgagc gggacgaccg
     1141 ggccgccgaa gggcgcgatg ctctcgcatc acaacgcggt gtggacggtc gactcgctgc
     1201 tgcaggtggt gcaggagcca ccgaacgccg ggcgccgcct gtcgttcctc ccgctgtcgc
     1261 acatcgccga gcgggtcacg tcgcacctct cgcagatcta caacgggctc gaggtctact
     1321 tcgccgagtc gctcgacacg gtgctgcgcg acctgcagga ggcccggccg accgtgttct
     1381 tcgccgtccc gcgcgtctgg gagaagttct acgccgggat caaggcgaag ctggcccagc
     1441 tcccggatga gcagcggcag atggccgagg gcgcgatcac cgtcgccacg gcggtcgtcg
     1501 agatggagca ggagggccag gaggtggtgg aggagatgcg ccacggcctg gaggccgccg
     1561 aggggatgct gttcgctccc ctgcgggcgc agctcgggct ggaccaggcc caggacctga
     1621 tctccggcgc ggcgccgatc aacgaggaca tcctgaagtt cttccacgcg gtcgggctcc
     1681 gcatccagga ggtctacggg cagaccgagg acaccggccc gacctcgctg aacccgaagg
     1741 agcggatcaa gatcggtacc gtcggcccgc cgatccccgg cctcgaggtg cgcatcgccg
     1801 acgacggcga gatcctggtg cggggcgaga acgtcttcca gggctacttc aaggacccgg
     1861 aggccaccgc cgagatgctg gaggacggct ggatgcgctc cggcgacgtc ggcgagctgg
     1921 acgaggacgg ctacctgcgg atcacggacc ggaagaagga cctgatcatc acggcggccg
     1981 ggaagaacat cgcgccgcag gagctggaga acctgctgaa gtaccaccca ctggtctccc
     2041 aggcagtggt gatcggggac cgccggccgt tcctgaccgc gctgatcacg ctcgacgccg
     2101 aggcgctggc gaagttcgcc gccgagcgcg ggctggggga cgtccccgcc ggggagctcg
     2161 ccgggcgcga ggaggtgcgg caggccatcg ccggggcggt cgacgcggtg aacgccgagt
     2221 tctcgcgggc cgagcagatc aagcgctggc acgtcctgcc gcgcgacttc ctgcaggagg
     2281 aggacgagat cacgccgacg ctgaaggtcc gccgccgggc gatcatcgag aagtacgggg
     2341 agacgatcga gtcgctgtac gcggccgggt gggagggctc gggctaagcg gtccagacga
     2401 gggccaggcg cacggcgttc aggccttcgg ggtcgctgcg gatcttgacg tccattgccg
     2461 cgacgaactc ctcgcggcag cgggacccgg tggtcgtgct ctggaagtcg cacagcgcgg
     2521 gggcggcggc ctcgaggacc gtctcccagg cccgggcgtg gttgcggatc gtcgtcttgt
     2581 cgaccgtccc gaggatcaga tggatccggg tctctgggta gtcgtagtcg gtgccgcccg
     2641 actcgacgcc gtcctgcacc cagcgcccgt ccttccacgc cggcgacgtg tcgtgccggt
     2701 agcagggcgg cgggttcggg tcgcccaccg tgtagcggta cccgtaggac tcgtcgatgt
     2761 cgatcaggtg cgacgcgtcc cattgatagc cggcctggtt caggcacccc gcccgcatgg
     2821 ccgcctgctg cggcccggac acggggacca cggcgtcgat ggcgagcttc agcgtcgtgt
     2881 ccgggccgta gtgggtcagc gcgtaggcca gcgcgtcggc gccgtcgctg ttgccgacca
     2941 cgcagaagcc gcactcgccg gtgggcgtcg tgatgttgag cggaacgtat tcctgggtgt
     3001 agatgcgatc gaggagcgcg acgggacggc aggccacgtt cgccatcccg gcccgatcgc
     3061 cgctcggggc gatgttccag gccgcgtacc agcgcacgtg gacgatggcc agcccggcat
     3121 tgatcagcgc cgtgcggaac gggacgctct gcggggtgtt cgaccagttg tagccggtgc
     3181 cctccccgcc ggacaggaac accaccatgc cgcggggcgt cgcggggttg cggtcgtcct
     3241 cggcgtacca ggcgcgaagc gtccgccagg tcccggacca gttgcaacgc acgtacaccc
     3301 cggtgcagtg gtggccggcc gggcatttcg ggtcggcctg gtcgttcggc agcacctggt
     3361 gcggcggcgg gccgggccac ggcgtgacgg ccccggcggg agggcccgtg gccgtgacga
     3421 cggtcgcgac gagcgcggtc gcgaggatcg tccggacggt ccgtaccccg gccctggaca
     3481 cctctaggtc agcaggaccg cgtcctgcag ctcgttcatg ccctgctggg agcccttgat
     3541 cgagtgcttc atgccgggga cgatgtcgag cgtgacctct ggggtgccgg ccgcctcgag
     3601 ggtggcctcc cagaactgcg cctgcttgtg cagcttgggg tccagctcgc cgacgaagat
     3661 gaccacacgg gtcgtggggt ggaagtggtc gccggcggtg tccaggccgt cctcggtcca
     3721 gcgggcggac caggcgtcgg tcaggttgtg cgtctcgcac ggcccgccgc ccccgacgta
     3781 tccgtacgtc gggtccaccc actcctcggc ttccgccccg tagttgaacc cttgctcgcc
     3841 gcggcagccc ctggccagct cggcgtggat cgggccgccg gtggggaaga ccccgtcgat
     3901 ccagctgtcc atcccgaagt gctcgatcag gtacacggga acggtgctcc ccatgctgct
     3961 gccgaccacg cagaacccgc accggccgtc ggcggggttc agcccggctg ctaccaacgg
     4021 ctggtagagg tccgtgtaga gccagtccac gaccgtcgcc gggcggcagg tcacggcccc
     4081 cggcccggcc agctccccgg agttcgactg gaaccacttg ccgttccagc gcacctgcgc
     4141 ggagatcatc cccgcgttga cgacgttgtc gatccacgcc ttcgagtagg ggccctcggc
     4201 gccccagtac cgcttcccgg agccgccgct gaaggtcacg accacgccgc gggtcgccac
     4261 gccggtgggc ggcctgcggg tgccgatgaa cagcttgcgc ccgccgatca ggcccgggca
     4321 gccggtgacc ttgtagacct tgcaggtgtg gccggggatc ttgacgcagt cggtggtgtc
     4381 ggtgccggca agggcggtga aggtaccgaa cgggcgcggg taggtgtcgg ccgtagccgg
     4441 gacggcgagc accgcggcga tcagggctgc cgccaggaac gccctctgga tcatggcgag
     4501 tggccggcga cgacgaggca cgcgcgaacg atacgcgtga tccgccacgg cgtccacggt
     4561 ttcctacccg acccggagga gggcctcgag gagggccttc atcccctcgg ccgacgcctt
     4621 gatcgaatgc cccatcccgg ggatgacctc ctcggtgacc tctgccgttc cggcggcgcg
     4681 caggcgggcc tcgtaggcgc gggcgtggtt gtggacggcc ggcttgtcgg atcctcccag
     4741 gatcacgtgc acgcgggtgg ccgggtggtg gtagtccttg ccgccggtgg cgacgccgtc
     4801 ggccacccag cggtcgtgcc aggaggcgtt ctcgcgcgcg caggggccgt ccgatccggt
     4861 gaagccgtag gtcgcgtcgg cctccgcggt atgggcctcg atgcggtagt cggggtcgcc
     4921 ctcgcaggcc gccgccagca tcgtgctggt cgggccggag gcgagcgcca ccatgtccag
     4981 catcccgtcc aggccgtagt acgccagcgg gtaggccgcc tgggccgacc cccagctgtt
     5041 cccgaccacg cagaacccgc agacgccggc cgcgtgccga ggcgccccga gctgcgggta
     5101 gagcgtctgc cgcaggtagc gcaccaccgt cgccgggcgg caggccaggc gggccagccc
     5161 gatccgctcc ccggccggcg ccatcgacca cttcaccgtc caccggatct ggatcgcgtg
     5221 caacccggcg tcgcggaggt cggccaggaa cgggccggcg ttcgggccgt cgcccagcca
     5281 ccaggtcttc cccgagccgc ccgggaacag cgccaccagc ccgcgcggcg accgcggctt
     5341 cgccggctcg gccaggaatc cgcgggcgac ccggctgatg cccgggcagc tcacgtcgaa
     5401 cccgcggcag gagtggcccc gcgggcaggt ggagtcgagg cggccgttca ggcggaacgt
     5461 cccggtcggc gacggcgcgg ccgccgcggg gggcgccgcg agcgccaccc cgagcgccac
     5521 cccgagcgtg gcgaggagga gcgccgccac aacggtcgcg gctcccgccg gacgccggcg
     5581 cgcgctcacg ccgggcaggc cgtcccgagc agcgcgtccc gcaacgcccg catcccctcc
     5641 ggcgacccct tgatgccgtg ccccatgtcg gggatcacct ggaagcagac ctgcggggaa
     5701 ccggcggcga gcagccggtc gcggtagtcg gcggcgtggt tcgggatgtt cacgtcgttc
     5761 tcgccgacga tgatgtgcac gcgggtggtg tcgtggcggt agtcactgcc gccggtggcc
     5821 acgccgtccg cccgccatcg cgccggccag tcgatcccct ggtaggcggg ctccgaccgg
     5881 cggcagggcc cggcgctgtc gatgtagccg tacgcccggt cgacgtccgt ctcgtagctg
     5941 ccctggtagc gatagccagg cgtgtccagg caccccttct ccagcgcccc gagcgtcggg
     6001 cccgaggtca ggacggcggc gtcgatcgag gcgtccaggc cgtagtggct gagcgggtag
     6061 gccgcctggt tcgtccccga gctgctcccg acgacgcaga agccgcaccg gccgaccttc
     6121 tcgttcggcg ccatgccggg gaccgggtac cggtgcgcgc ggatccagga cagcgccgtg
     6181 gccgggcggc atccgagcgc cgacgccccg gcgcgctcgc cggacagcgc gacggcccac
     6241 ggcgtctgcc agcgcaccat gacgacgcga agcccccggt cgaccagctc ggccaccagc
     6301 gcccgggcgg tgtcgtgcgt ttccgtccag tagcgcttgc cggagccggc gttcaagaac
     6361 accaccaggc cgcgcggcgc ggcgggccgc gggccggtgg cgacgaaccc gcgggcgtcc
     6421 ttccggacgg cgggacagtg cacctcgatc ccctggcagc cgtagccgct cgggcaggac
     6481 ttgtcgaccg cggccgtggt ggccagggtg ccgaggggaa gaggccccgc ggccgccggg
     6541 gtggccggga gcgccgccac cgccagggcg aacacggcga gctgcgccgc gcgacgcgcc
     6601 cgacggcccg gggcgggcga gaccggctcg atcaccccgg cctacgaggg gggcggcacc
     6661 gcggtcggcc aggtgatcgc cctggtcatc gccagcatcc cgagcggcga ctccttgatc
     6721 gagtgcgaca tgtacgggac gaagtactcg aagcagtggc tccccagcac gcccgcgctg
     6781 aagtcgcagc ccgcggtgta ctgctccacg acgccctgcc acacctcgga gtggtgccag
     6841 accgggtggt tgtcgacggc gccgaggagg acctcgatcc gcgtgtccgg gtggttgtag
     6901 gtgccctggg gcgcgccgta gggggtggcg ccgtaggccg aggtgttggc gatcccgtcg
     6961 ttgcgccact tcggcggcca gttgaccccg gcggggttgg tgttggcgca cggcccctgg
     7021 acgccgatgt agccgtagga ctcgtccacg tccacgtagt ggtcgggcgc catcttgaac
     7081 tcaccaacgc ccttcccctg gcacgccagg tcgagcgccg tgtagatcgg ccccgaggcg
     7141 atcacgacgg cgtcgggcac cgcgttcacc gcggccagct gcggcgtgta gtggctgatc
     7201 gagtacacgg cctccgccgt gccggcgctg tagccgctga cgcagaaccc gcattcgaac
     7261 ggggtcgtcg gcgcgttccc cggcgtgagc accttcggga cgtagatgtt ctggtagacg
     7321 tgcttcacga cctccgagct gcggcacgcc aggtcgtcga accccgccga gcggtctccg
     7381 gagggcgccg cgttccacgt gccattccag cgcaggtcga cgacgtagat ccccgcgtcc
     7441 atcatcttgt tcacgtagta ctggttgctg gtgttcttct ccgggtacca gtaggtcttg
     7501 cccgagcccc cggagaagaa gaccgccact ccgcggggct cgaccgtgaa ctcccgcgtt
     7561 ccgcccacgt agccggggtt gttcttgttc tccgcgatcc agagcttggt cttgcgcacg
     7621 ccggcgcccc agtcgcacac ggccttgtag gccttgcact tgtacccgac ggggcacttc
     7681 gaatccgcgt acttgatctc ggggagcagc tcctcgagcg gctgcagcgt gggaacagga
     7741 tcggtcgtcg ccgcagcggg tccgggcgta agagctccgg acagcccgat cagcagtgcc
     7801 accatggcca cggcgatggc ccggcgacgg ggggcggctc gcattcggtt cctcctcaga
     7861 tggtgaaggt gatgcaggac gttcggatgg gtctcgcgcc cgaggataat acgtacccgg
     7921 ctggcgggat gggagtaatc caagcccgcg tcacgtcaaa gggtctccgg ccagatcacg
     7981 ccgcgggtga ggttcgccag gccggcgagc gagtcgcgga tggcgtggcc catccccggg
     8041 atcaggatct cgtagcaggg ttcgccgatg acgtcgccgc cgctcgtctg gaagtcacag
     8101 tcacccggcg acgcgttctc gagctgcccc tcccagtcgc gggcgtgctt ccacaccgac
     8161 tccttgtcca gctttccgag gatgatgtgc acccgggtgt tcgggaacgc cgtgtagatc
     8221 gctccggtgt ccacgccgtc gttcggccac cgggtctgcc gccagatggc gagggtgctg
     8281 tagctcacgc aggggccggt cccagaggtg tcgccgtagg acttgtcgac gtccgcccgg
     8341 tgcgtcttgt cgtagtagta gccgtagacc ttcgtgcatg ccttgaccat gcccgcgagc
     8401 gtcggtccgg cggcgaagaa cgcggcgtcg acgaacgcat tcgccccggt tccgtccccg
     8461 gccaccccgt acacggtcat cgggtaggcc acctgctcgg tcccgtagct cgtcccggcg
     8521 acgcagaacc cgcactcgaa ctgcgggtgt ggcacttgat ccaggtggaa ctcctgctgg
     8581 tacaccttct ggtagaccca tgtgatcagc ccgtatggcc ggcagccgag gtgggcgaag
     8641 ccggcgctca cgtcgccgct cggtccgagc atccagttgc cgttccagcg ggcctggacg
     8701 acgtggatgt tgtggttctt caggtcggtg aacatctgcg ggctggcgtc gcccgtgccc
     8761 cacgtatacc ccttgccgtt gccgccggag aggaacacga cgaccccgcg cgggctcgtc
     8821 agtccgtcgt tgcgctcggc gaaccaggcc ttcgagttgc gccaggtgcc gttccagttg
     8881 cacttgacga tgacgccggt cccgttgcac cgcgatccgg tcgggcactt cgtgtcgttc
     8941 gccagcgagc ccgtgagcgg tacgagctgc atctgcgccg gggtggccac cgcctccgtt
     9001 gcggccggca cggcggctcg ccccgtggcc ggcgacagga gggcgaggac gaggagcggg
     9061 aggagggcgc gaggcacgag aggcacggcc agagcgtaga ggttgccgtc cggctcgtcc
     9121 agataccggg ctagcccggg ttgtcgacgc gggccgtggt cagggcgcgc agcagcgagt
     9181 cgagcccctc cgcggacccc ttgatgccgt gccccatgcc ggggatgacg gcgaagccga
     9241 cctcgggggt ccccgacgcc accaggcgct cgaagtaggg gtgtgccagg ccctgcacgc
     9301 cgtggtcgcg ttcgccggcg atgaactgca cgcgggtgtc cgggtagtcg tacgcggcgc
     9361 cgcccgttgc cacgccgtcc tccagccagc gctcctccca ggacgggtcg tgggcgcggc
     9421 aggggccctc gccgttcgcg tagccgtagg cgcggtcgat cacccgcgcg tacgccccgt
     9481 agccctcgcc gcccccgatc aggcacccgg ccgccagcgc gccgtgcacg gggccggagg
     9541 tcaggacggc cgcgtcgacg aggtcctgca gcccgtagtg cgtcagcgcg tacgccacct
     9601 gggaggcgcc gctgctgctg ccgacgagac agaacccgca cgcgccggcc cccgcctcgg
     9661 gagtcaggcc gaggtccgga tagagcgtgt cgtgcatgaa gcggatcgcg gtggcggggc
     9721 ggcaggcgag cgccgccggg ccggcgtcct cgccgggctc ggcgagctgc cacgggatct
     9781 gccagcggac ctccaccacg accaggccgg cgtcgaccac gctccccagg aagtcctgcg
     9841 gggcctgctc ctcgccccag taccgcttgc ccgggccggc gttgaagaac accgccatcc
     9901 cggccgcgcc gtcggggatc tcggacacgg cgtagaaccc ggggtcggcg cgctccacgc
     9961 cggggcagtc aaccgtgaac gcgtgacagg tgaagccggg cgggcacatc gagtcgctgc
    10021 cgccctcctc ggtgaggtcg ccgaggggca ggggctcggc gcgggggatg acggtcggcg
    10081 ggccgctgcc ggagggcagc gtgccggggc tcccggtggg gctgccgccg tgcccgccgc
    10141 ggggggaggt gcgctgcggc ccgatcggga ccggcttcgg gctgcacgcg gcgagcgcca
    10201 ggcccgcggc tagcgcggac agcacggcac gacggatgct gggggaggtt cggatcatcg
    10261 gtgtcgagtg gtggccgggg ccgtgccacc atgtacccgt tccccgagcc tacatgcgag
    10321 gacccggagt gacgatgaga gaacgacggt gatgcgcagc cggcacgttt ccacggtcga
    10381 tcaggggttc gtcccggcca ccgccgcggt ttgctacggc cggctggccg acccggccgg
    10441 ctacccggcg tggtggccgg gggccgccgt tcgcgacgcg ccgggcggga cggcgataga
    10501 gatcggcggc ctgcgcatgg agggcgtccg gccggacggc gtccggcccg gcgtgggcct
    10561 gtacctgcgc ttcaacgggc ggggcggcgc ggggagcctg gagtggtacc tggagccctt
    10621 cgacgggggg acgatcgtca gcgccatcgc cgagctcgac ccccggcgcc cgtggggccg
    10681 gcggcgcgtg ctgcgcttcc gggcggcgat ccgcgacggg ctggtcggcc tgcgcgcgtt
    10741 cgcggaagcg aacgcgtgag cgagcggcgc tgcccgcgct gccgggcgct cgtcggg
//
LOCUS       DASPWO010000255         8620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_120096, whole genome shotgun sequence.
ACCESSION   DASPWO010000255 DASPWO010000000
VERSION     DASPWO010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120096"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="VEO00_05705"
     CDS             complement(<1..169)
                     /locus_tag="VEO00_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HYU57528.1"
                     /translation="MVEDPRLTLLLALADDELIIGHRHSEWTGWAPHIEEDLAFSSIA
                     QDEMAHARLLYG"
     gene            210..788
                     /locus_tag="VEO00_05710"
     CDS             210..788
                     /locus_tag="VEO00_05710"
                     /inference="COORDINATES: protein motif:HMM:NF023502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 family protein"
                     /protein_id="HYU57529.1"
                     /translation="MGSRTRRAITPVVALVVLAGCSNGTPTAQGSPSEAGSATGSTTT
                     SASPGASPLEAGPVHYYECASLLSDQEVQAATGMADAAFLSEEQGEAVEGQTYCQFFA
                     GQGAISIAVSVDTGPAFLVYQQLEAAGAALPDVPGLGDAAKWSEQGGTLGVRVGNVGL
                     TIAFTNLGGGALGIADAQGAAVDMATLILSRL"
     gene            complement(793..1173)
                     /locus_tag="VEO00_05715"
     CDS             complement(793..1173)
                     /locus_tag="VEO00_05715"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57530.1"
                     /translation="MEVWDVLGKRTDDDELEVVGGVNAPDRALALLLAHEAYFRHREG
                     VGFAVRRRGDGAVHVYDGGAPLGGVTDRSYRRQDGYVGVGAKLKRVREEMRRRGVVVD
                     RPRPPGRGRDADAGRDAGRDSDDG"
     gene            complement(1189..2103)
                     /gene="paaA"
                     /locus_tag="VEO00_05720"
     CDS             complement(1189..2103)
                     /gene="paaA"
                     /locus_tag="VEO00_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HYU57531.1"
                     /translation="MTYAERVAEFRARIDGGEKVEAGDWMPDEYRQAALKFIEMHANS
                     EVMGALPEREWIPRAPTLRRKLSLTAKVQDEVGHGQILYRVAEDLGKSRAAMFDDLVN
                     GRTKFHNVFHYPTETWGDVAVIGWLIDGAALVTQKALLDASYMPYVRAMRRICPEESL
                     HLRHGEDLTLELCSGTDLQRAMFQDAVNRWWRPIMHFFGPPSNPAKDVLLYWGIKTRS
                     NEDLRQEFFSTYVPKVWDLGISVPDPVLAWNAARDEWDWSDPGWDDFWEVVRGRGPMT
                     SVRLLYRKSMWDTHAWVREAFSGIPRAA"
     gene            2230..2532
                     /locus_tag="VEO00_05725"
     CDS             2230..2532
                     /locus_tag="VEO00_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57532.1"
                     /translation="MKESPATRGQMFGMPVLKIGTKVFTGVWGDAMNFKLGEKERAAA
                     LKLDGAEQFDPGMGRPMKEWVLVPASHAARWTRLAKQALAYVWAGSTPPTSATRRT"
     gene            complement(2576..3886)
                     /locus_tag="VEO00_05730"
     CDS             complement(2576..3886)
                     /locus_tag="VEO00_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HYU57533.1"
                     /translation="MSEPVPADPALHRLAEALDPAGMRAFIRRCLRRPGWEIEGCIVD
                     RVRYRRGVRAVVGYRVRVVNGSTNESRDHWVTALLNRPDRTRHVWKRLQHVIPGAAEA
                     DDLPLAPAAMASDESLVLQTFPIDRRLPSLLALLHPERLISAALPSGEDTMGPWVVEP
                     VRYRPGLGCAMRWRTSLGTSRGTTRYVKAYRDDQGAATEKVLASLAPRRGDGFAVPAP
                     VAYLSEHRALVQEAIAGQTLTDLIVGGGDPGPLMERAARALIEFQRAPALTASRRTRR
                     DHLADLGRSVTLVSWVRPSLAAVARDVMACVADRLPEAEPFAAHGDLKPDHVIVGDER
                     MALVDLDAYARSDPVADAGAMLGRLAGLAVRRPWVAERIHAAERSFTETYFDAAPPGW
                     EARLPAHLSAAQLSEAAACFRHQVAEWPVHMDTLTRRAAEALGG"
     gene            complement(3883..4824)
                     /locus_tag="VEO00_05735"
     CDS             complement(3883..4824)
                     /locus_tag="VEO00_05735"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYU57534.1"
                     /translation="MRGLPCMFVVGCPRSGTTLLQRMLDHHPQLAVANDTHFIPRAVD
                     PAGGDVPLSPALVERVLRYHRFPRLGVPEADTRRAAAGARTYAEFVGTLYREVAARRS
                     KPLAGEKTPDYVRYLAFLGRLFPAARFVHIVRDGRDVTLSTLQWARPDKGPGRFSLWR
                     QDPVATCALWWRWQVTTGRSEGEALGARYREVRYEDLVRRPRETVEGLCRFLDLPFAA
                     EMLDYHVGRTRRGAGLSAKRAWLPPTPGLRDWRTQMADGDVEVFESLAGDLLTSLGYA
                     RRTTAPSRAVAARAERAEAGWEAELERRAKRRASARS"
     gene            complement(4821..5705)
                     /locus_tag="VEO00_05740"
     CDS             complement(4821..5705)
                     /locus_tag="VEO00_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HYU57535.1"
                     /translation="MHWPADHPAITDPALSPWAGEVDSLCPEAEVVRLLRHLPGRRAT
                     TMVRSEAGPAVLKLFATSRARGGHRRLAAFGETAAADLVPRALGHRKGHLALVEFVPG
                     TPLVDLVGDEPVRSCAVAGLALRRLHASGADLGRTWGPDDEIDQLRRTAGPVTRHGVE
                     QAARWARPGARAVVPSHRDCHPAQVIRTASGVRFIDLDDCAMAPPALDVGNFVAHLQM
                     EGHLGRWSREVAGAAAAAFMEGYGSEPDGLAWWNLVSLARLAALAETRHGWPSRARGL
                     LELLAETRAGATARTGPA"
     gene            complement(5689..6861)
                     /locus_tag="VEO00_05745"
     CDS             complement(5689..6861)
                     /locus_tag="VEO00_05745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYU57536.1"
                     /translation="MRIAVLCSDTGIRVPDTKGASLHLVAVASALARIGHDVMLIGVA
                     GHGGPPSGMEALLVRHPGWSKGIRRELRKLSTVERLVREARDPLARFVPDIVYERLAL
                     FGTAGVRLAAAVGAPHVIEVNALLAEEEARWRGLRLAGMARRRERVVLRSADLRVAVS
                     DEVASAVEATAPGRPTAVLPNGVDGALFARLPGAAEARAFLGLPADAPVVGFAGSLRP
                     WHGLEVAVEALRELPGVVLAVAGEGEVRAGLEERARRLGVADRVVWIGQLPHQLIPGF
                     LAALDVAVLPYPALPDFAFSPLKLYEYLAAGIPVVASDLGQVRAVLEDGQGVLVPPGQ
                     PAVLADAVREVLGDLGAARERAARVRRVALEEHTWERRARSLTEFVQRGRVGALAG"
     gene            complement(6858..8051)
                     /locus_tag="VEO00_05750"
     CDS             complement(6858..8051)
                     /locus_tag="VEO00_05750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYU57537.1"
                     /translation="MTSGPILYVLKRFPRLSETFVLREILTLEALGERILVDSLLPAE
                     DGPRHPDLDRVQAEVRYLPKHPRLRDPSVRRSHLRLALRRPARWLRLATRARRAGTWR
                     RFLQAGLSADRARREGVRHIHAHFLTAAAEVARDAAALSGIPLTATAHAKDIFHKDNV
                     PLIRSRVRGAAALVTVSEHNVGYLRRVIGNGMPIRFVPNGVALASPANDRPNGPVLCV
                     ARLVPKKGVDTLIDAGALLTTRLPGLSIEVVGDGPEAQPLCERVRTLGMTDRVRFLGQ
                     RPWDDVERAFRRCSIVALPCRVDANGDRDGMPTVLVEALALGLPVVSTDVAGISELVR
                     DGETGLLVRPDDPAALADAIDRLARDRPLARRLGRAGRALVAERFDPMGSARLLQRVF
                     EGVRA"
     gene            complement(8048..>8620)
                     /locus_tag="VEO00_05755"
     CDS             complement(8048..>8620)
                     /locus_tag="VEO00_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU57538.1"
                     /translation="YDPQRGSVSIDGRDVRDSTLASIRSQVALVLQDSVLFHGTLWDN
                     VACGRPDAAPDDVTRAIRLALVDEFADRLPDGLGTMLGERGVNLSGGQRQRIAIARAI
                     VRDAPILILDEPTSALDAGSEQLVVDALRNLMQGRTTLVIAHRLSTIRRADRVIVLKD
                     GAVVEEGSHAELMRARGEYAHVAMLQGVGP"
BASE COUNT         1228 a         3204 c         3087 g         1101 t
ORIGIN      
        1 gcccgtacag cagccgggcg tgggccatct cgtcctgggc gatcgacgag aacgccaggt
       61 cctcctcgat gtgcggcgcc cagccggtcc actccgagtg acggtgcccg atgatcagct
      121 cgtcgtcggc gagcgccagg agcagggtga gccgcggatc ctccaccatc tctctatgct
      181 acgagtccca cagacggcga ggaggccggt tgggatcgcg gacccggcga gcgatcacgc
      241 cggtggtggc cctggtcgtc ctggcgggat gctcgaacgg gacgcccacc gcccagggtt
      301 ccccgagcga ggcagggagc gcgacgggga gcacgacgac ctccgcctcg ccgggcgcct
      361 ccccgctcga ggcggggccg gtgcactact acgagtgtgc ctcgctcctc tccgaccagg
      421 aggtccaggc ggccaccggc atggcggacg ccgcgttctt gagcgaggag cagggagagg
      481 ccgtcgaggg ccagacctac tgccagttct tcgcggggca gggcgcgatc tcgatcgccg
      541 tctccgtcga caccgggccg gcgttcctcg tgtaccagca gctcgaagcg gcgggggcgg
      601 cactccccga cgtccccggc ctcggcgacg cggcgaagtg gagcgaacag ggcgggaccc
      661 tcggcgtgcg ggtggggaac gtcgggctga cgatcgcctt caccaacctc ggagggggcg
      721 cgctcggcat cgccgacgcg cagggcgcgg ccgtggacat ggcaaccctg atcctgtcgc
      781 ggctgtagcg actcagccgt cgtcagagtc ccggcccgcg tcccggccgg cgtccgcgtc
      841 gcggccacgt ccgggcgggc gggggcggtc gacaaccact ccgcggcggc gcatctcctc
      901 gcggacgcgc ttcagtttcg cgccgacccc cacgtagccg tcctggcggc ggtacgagcg
      961 gtcggtcacg ccgccgagcg gagcgccgcc gtcgtagacg tgcaccgcgc catcgccccg
     1021 ccggcgaacc gcgaacccga cgccctcgcg gtgacggaag tacgcctcgt gcgccaggag
     1081 gagggccagg gcgcgatccg gcgcgttcac cccgccgacg acctccagtt cgtcgtcgtc
     1141 ggtccgcttg ccgaggacgt cccagacctc catccgagat cgctgccgtc aggcggcgcg
     1201 tgggataccg gagaaggcct cccggaccca ggcgtgcgtg tcccacatcg acttgcggta
     1261 caagagccgc acgctcgtca tcgggccgcg gccccggacg acctcccaga agtcgtccca
     1321 gccgggatcc gaccagtccc actcgtcgcg cgccgcgttc caggcgagca cggggtcggg
     1381 cacggagatg ccgaggtccc acaccttcgg cacgtaggtg gagaagaact cctggcgcag
     1441 gtcctcgttc gagcgggtct tgatccccca gtacagcaac acgtccttcg ccgggttcga
     1501 gggcggcccg aagaagtgca tgatcggccg ccaccagcgg ttcacggcgt cctggaacat
     1561 cgcccgctgc aggtcggtgc cgctgcacag ctcgagcgtc aggtcctcgc cgtggcgcag
     1621 gtgcagtgac tcctccgggc agatccggcg catcgcgcgg acgtagggca tgtaggacgc
     1681 gtcgagcagc gccttctgcg tgacgagcgc ggcgccgtcg atcagccagc cgatcacggc
     1741 gacgtcgccc cacgtctcgg tcgggtagtg gaagacgttg tggaacttcg tccggccgtt
     1801 gacgaggtcg tcgaacatcg ccgcgcggct cttcccgagg tcctcggcca cccggtacag
     1861 gatctggccg tgcccgacct cgtcctggac cttcgcggtc agcgagagct tgcggcgcag
     1921 cgtcggcgcg cgcgggatcc actcgcgctc gggcagcgcc cccatcacct cggagttcgc
     1981 gtgcatctcg atgaacttca gcgcggcctg gcggtactcg tccggcatcc agtcgccggc
     2041 ctcgaccttc tccccgccgt cgatccgcgc ccggaactcc gcaacccgct ccgcgtaggt
     2101 catcctcgac cccccttcag ggcacaatcg tactcgccgg ggacgggtag tctcttcgcc
     2161 gtgccgtcga agatgccgca gccttcgccc gagacctcgg cgcggttcga tcgcgttgtc
     2221 gcagctctca tgaaggagtc gccggccacg cgcggccaga tgttcggcat gccggtgctg
     2281 aagatcggca cgaaggtctt caccggggtc tggggcgacg cgatgaactt caagttgggc
     2341 gagaaggagc gcgccgcagc gctgaagctc gacggcgccg agcagttcga tcccgggatg
     2401 ggccgcccga tgaaggaatg ggtgctggtc cccgcgtccc acgccgcccg ctggacccgc
     2461 ctggccaagc aggccctcgc ctacgtctgg gcggggtcca ccccgccgac gtccgccacg
     2521 cgccggacct gacccgctcg gtccccgggc ggcgctccga ctcgagccgc cgggatcagc
     2581 cgccgagggc ctcggcggcc ctcctggtga gggtatccat gtgcaccggc cattcggcca
     2641 cctggtggcg aaagcacgcc gccgcctcgg acagctgggc ggctgagagg tgggccggaa
     2701 ggcgagcctc ccagcccggc ggtgcggcgt cgaagtaggt ctcggtgaac gaccgttccg
     2761 ccgcgtggat ccgctccgcc acccacgggc ggcgaacggc gaggcccgcc agccgtccga
     2821 gcatggcgcc ggcatcggcg acgggatccg accgggcgta ggcgtcgagg tccacgagcg
     2881 ccatcctctc gtcgccgacg atcacgtggt cgggcttgag gtcgccgtga gccgcgaacg
     2941 gctccgcctc gggcagccga tcggccacgc acgccatcac gtcgcgggcc accgccgcca
     3001 gcgagggccg gacccacgac acgagggtga cggaccggcc gaggtcggcg aggtgatccc
     3061 ggcgggtacg ccggctggcc gttagcgcgg gggcgcgctg gaactcgatc agcgcacgtg
     3121 ccgctcgttc catcaacgga cccgggtcgc cgccgccgac aatgaggtcg gtcaacgtct
     3181 ggccggcgat cgcctcctgg accagggccc ggtgctccga caggtacgca accggggccg
     3241 gcaccgcaaa cccgtcaccg cgacgaggcg ctagggacgc cagcaccttc tccgtcgccg
     3301 cgccctggtc gtcccggtag gccttcacgt atcgcgtggt cccccgcgac gtgccgaggc
     3361 tcgttcgcca ccgcatggcg caccccaggc ccggccggta tcgaaccggc tccacgaccc
     3421 agggccccat ggtgtcctca ccggacggca gggccgcgga aatcagtctc tccgggtgca
     3481 acagggccag cagcgacggc agccgccggt cgatggggaa ggtctgcagg acaaggctct
     3541 cgtctgacgc catcgcggcc ggcgccaacg gaaggtcgtc ggcttcggcc gctccgggga
     3601 tcacgtgctg caggcgcttc cacacgtggc gggtccggtc cgggcggttc agcagcgccg
     3661 tcacccagtg atcgcgggac tcgttcgtgg agccgttgac gacacggacg cggtacccga
     3721 cgacggcgcg gacgcctcgg cgatacctga cgcgatcgac gatgcatcct tcgatctccc
     3781 atccggggcg ccgcaggcac cgccggatga acgcgcgcat accggccgga tccagggcct
     3841 cggcgagacg atggagggca ggatcggcgg ggaccggctc gctcacgatc gagcgctcgc
     3901 ccgccgcttt gcgcgccgct cgagctcggc ctcccatccg gcctcggcgc gctcggctcg
     3961 cgcggccacc gcccgggacg gggccgtcgt tcggcgcgcg tatccgaggg acgtgagcag
     4021 atcgccggcg agggactcga acacctcgac gtcgccgtcg gccatctggg tgcgccagtc
     4081 gcggagcccg ggggttggag gcagccatgc ccgcttggcc gacaggcccg ccccccgccg
     4141 cgtccggccc acgtgatagt cgagcatctc ggcggcgaac gggagatcca ggaagcggca
     4201 caggccctcg accgtttccc gcggccggcg taccaggtcc tcgtaccgga cctcccggta
     4261 gcgcgctccc agggcctcgc cctcggaacg gccggtggtc acctgccatc gccaccagag
     4321 cgcgcacgtc gcgacggggt cctgcctcca cagcgagaac ctgcccgggc ccttgtccgg
     4381 ccgggcccac tgcagcgtcg acagcgtcac gtcgcggcca tcgcggacga tgtgcacgaa
     4441 ccgtgccgca gggaacaggc ggccgaggaa cgccaggtag cggacgtagt cgggcgtctt
     4501 ctcgcccgcg agcggcttgg accgccgggc cgcgacctcc cggtacagcg ttcccacgaa
     4561 ctcggcgtac gtacgcgcac cggcggcggc tcggcgagta tcggcttcgg ggaccccgag
     4621 gcgcgggaac cggtgatagc ggagaacgcg ctcgacgagc gccggcgaga gcggcacatc
     4681 gccccccgcc ggatcgacgg cccgggggat gaagtgcgtg tcgttggcga cggcgagctg
     4741 cggatggtgg tcaagcatcc gctgcaggag ggtcgtgccc gatcgcgggc agccgaccac
     4801 gaacatgcac ggcagccccc tcacgccggc cccgttcgcg cggtggctcc cgcgcgcgtc
     4861 tccgcgagga gctcgagcaa tccgcgagcc cgcgacggcc atccgtgacg ggtctcggcc
     4921 agcgccgcca gcctggccag gctcacgagg ttccaccagg ccagcccgtc cggctcggac
     4981 ccgtagcctt ccatgaaggc cgccgcggcg gccccggcca cctcccggga ccatcgaccc
     5041 aggtgtcctt ccatctggag gtgggcgacg aagttgccga cgtccagcgc gggaggcgcc
     5101 atcgcgcagt cgtccaggtc gatgaatcga acacccgagg cggtccggat cacctgggcc
     5161 gggtggcaat cccggtggga cgggacgacg gccctcgctc ctggtcgggc ccagcgcgcg
     5221 gcctgctcca cgccgtgccg ggtgaccggt ccggcggtgc gccggagctg atcgatctcg
     5281 tcatccggcc cccacgtccg gcccagatcg gccccgctcg cgtggagccg gcgcagtgcc
     5341 aggccggcca cggcgcagct ccgcaccggc tcgtccccga cgaggtcgac cagcggcgtc
     5401 ccggggacga actccaccag tgccaggtgg cccttgcggt gccccagtgc ccgcggcacc
     5461 agatcggcgg cggccgtttc gccgaacgcg gcgagcctgc ggtgcccgcc cctggcccgg
     5521 gacgtcgcga agagcttcag cacggccggg ccggcttcgc tccggaccat ggtggtggcc
     5581 cggcgcccgg ggaggtgccg gagcagccgg acgacctcgg cctcgggaca gaggctgtcc
     5641 acctcgcccg cccagggaga cagggcggga tcggtgatcg cggggtgatc agccggccag
     5701 tgcaccgacc cggcctcgct ggacgaactc ggtcagcgat cgagcccggc gctcccacgt
     5761 gtgctcctcg agggccaccc ggcggacccg cgcggcgcgc tcccgggccg cccccagatc
     5821 gcccaggacc tctcgaacgg cgtcggccag cacggccggc tgtcccggcg gcaccaggac
     5881 gccctgcccg tcctccagga ccgcccggac ctgccccaga tcgctggcca cgaccgggat
     5941 cccggcagcc aggtactcgt acagcttgag gggggagaag gcgaagtccg gcagggccgg
     6001 atatggcagc acggccacgt cgagcgccgc caggaacccc gggatgagct ggtgcggcag
     6061 ctgtccgatc cacaccacgc ggtcggctac gccgagtcgg cgagcacgct cctccagccc
     6121 ggcccggacc tcgccctcgc ctgccaccgc caggacgacc ccgggcagct cacgcagcgc
     6181 ttcgaccgcg acctccaggc cgtgccacgg ccggaggctg ccggcgaagc cgacgaccgg
     6241 cgcatcggcg ggaagcccga ggaatgctcg tgcctcggcg gccccgggga ggcgggcgaa
     6301 cagggccccg tcgacgccgt tgggcagcac ggcggtgggt cggccgggtg cggtggcctc
     6361 cacggccgag gccacctcgt cgctcaccgc gacgcgaagg tccgcgcttc ggaggacgac
     6421 tcgctctcgc cggcgggcca taccggcgag cctcaggccc cgccaccgtg cctcctcctc
     6481 ggcgaggagg gcgttcacct cgatgacgtg gggcgctccc accgccgccg cgagccgaac
     6541 gcctgccgtg ccgaacagcg ccagccgctc gtagacgatg tcggggacga accgcgccag
     6601 gggatcccgg gcctcccgaa ccagccgctc cacggtggag agcttccgaa gctcccggcg
     6661 gatgcccttc gaccaccccg ggtggcggac cagcagtgct tccatccccg aaggaggtcc
     6721 accgtggccg gcgacgccga tcagcatgac gtcgtggccg atccgggcca gggcgctggc
     6781 gacggccacc aggtgcagcg aggcgccctt cgtgtcgggg acgcggatcc cggtatcgga
     6841 gcagaggacg gcgatcctca tgccctcacc ccctcgaaga cccgctgcag gagccgggcg
     6901 ctgcccatcg gatcgaaccg ctcggccacg agcgcgcgcc ccgcccgccc cagccgccgg
     6961 gccagcggcc tgtcgcgcgc gagccggtcg atcgcgtcgg cgagggcggc gggatcgtcc
     7021 ggccggacca acaggcccgt ttccccgtcc ctgacgagct cggagatgcc cgcgacgtcg
     7081 gtggagacga ccgggaggcc gagggcgagc gcctccacga ggaccgtcgg catcccgtca
     7141 cggtcgccgt tcgcgtccac gcggcacggc agcgcgacga tcgagcagcg acggaaggcg
     7201 cgctcgacat cgtcccacgg ccgttgcccg aggaagcgaa cccggtccgt catcccgaga
     7261 gtgcggacgc gctcgcacag cggctgcgct tcgggaccgt cgcccacgac ctcgatcgag
     7321 agcccgggca gcctcgtggt gagaagggca cccgcgtcga tcagcgtgtc gacacctttc
     7381 ttgggcacca accgcgccac gcacagaacc ggaccgttcg gccggtcgtt cgccggcgag
     7441 gcgagcgcga ccccgttggg tacgaaccgg atcggcatcc cgttgccgat cacgcggcgc
     7501 aggtacccga cgttgtgctc ggacacggtg accagcgccg cggcgccccg gacccgcgat
     7561 cggatcagcg ggacgttgtc cttgtggaat atgtccttcg cgtgggcggt ggccgtgagg
     7621 gggatccccg acagggcggc ggcgtcccgg gcgacctccg ccgcagcggt caggaaatgg
     7681 gcgtggatgt gccgaacccc ctcccgccgg gcccggtcgg ccgacagtcc cgcttgcagg
     7741 aaccgccgcc aggtcccggc ccggcgcgcc cgcgtcgcca gccgcagcca gcgggccggg
     7801 cgccgcagcg ccagccggag gtgcgaccgg cggactgacg ggtcgcgcag gcgcgggtgc
     7861 ttcgggaggt agcgaacctc ggcctgcacc cgatcgaggt cgggatgccg gggaccgtcc
     7921 tctgccggga ggagcgagtc gaccaggatc cgctccccca gcgcctcgag cgtcaggatc
     7981 tcccgcagga cgaaggtctc cgagaggcgg gggaaccgct tcaggacgta gaggatcggc
     8041 ccggaggtca tgggccgacc ccctgcagca tggccacgtg ggcgtactcg ccccgggccc
     8101 gcatcagctc ggcgtgcgag ccctcctcca cgaccgcgcc gtccttcagg acgatcaccc
     8161 gatcggcccg gcggatcgtc gacagccggt gagcgatcac gagggtcgtg cggccctgca
     8221 tcaggttgcg cagggcgtcc acgaccagct gctccgagcc cgcgtcgagg gcgctggtgg
     8281 gctcgtccag gatcaggatc ggagcgtcgc ggacgatggc ccgggcgatc gcgatccgct
     8341 gacgctggcc tcccgacagg ttcaccccgc gctcgccgag catcgtcccg aggccgtccg
     8401 ggagccggtc ggcgaactcg tcgaccaggg ccagccggat ggcccgcgtg acgtcgtcgg
     8461 gcgcggcgtc gggccggccg caagcgacgt tgtcccagag cgtgccgtgg aagaggacgc
     8521 tgtcctgcag caccagcgcg acctggcttc ggatcgacgc cagcgtggag tcgcggacgt
     8581 cgcggccgtc gatcgagacc gagccgcgct gcgggtcgta
//
LOCUS       DASPWO010000256        10344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_549248, whole genome shotgun sequence.
ACCESSION   DASPWO010000256 DASPWO010000000
VERSION     DASPWO010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10344
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549248"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..3762)
                     /locus_tag="VEO00_05760"
     CDS             complement(385..3762)
                     /locus_tag="VEO00_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HYU57539.1"
                     /translation="MTSGPRQHDDPGGRSAWFHRSRGGRRGLPGEAFLRAATARREAA
                     FAPAAAAAPAAPAAPGGAVNWIPLGPFAITGGQASGHPVVSGRIEAIAAGPGGTRVYI
                     GAADGGAWFSPDGGATWEVLDDFATTAPGTPGRVANSLSVGALAVAFGADRAGDQVFV
                     GTGEYRAGYRGLFGIGVRVSTAGGQLGSWSLEGTNLAGLGCTKLVIDPDDPALVVVAT
                     TRGLITRPAAAAARATWTQVASPAFTNGLGVVSDVLIAGAGAAKTYYAAFENDGVYSS
                     PDLAAWSRVPAAAVGGGPAAGDGRIVLAASEVTPSVVYAFCQSRRLFRLTAGSFAAVA
                     GVPTNVFGTTGTGWYNIILAVDPAAADTVYLGGSVAWDLAPGDPVADWTLALFRGTVT
                     NPAVTPAFPFNAANNGPGTAGVFRESTDPTFVGRGIHADAQSIAFSRRADNTGLDGAG
                     VWIGCDGGIFHSAAAGALGTFTSRGAGLSTAQMTYLAAHPTLEAVALAGCQDNGTIRW
                     TSGPTWHETLVADGGGVAIDPNNPYRMMRQYIRASLSQTATGGATRANWTALNFPPRT
                     ANTVAQGNAAATEGGGANSDFYSPIATTPAGVVPTLVAKGTHRVWLSSNWGTSWVTLP
                     TGTNPYAAAVPSLVQDTLAGRVRGLAFPSAVLLFAATATSVFRFDLAAGVWSRTTLPA
                     IPGVAAATPITSIASAGGTTLYATVGGLGVDHCWYFDGAAWHATGLMLMPGGGAGTPQ
                     VVDIPASCVAIDPANPTTVFAGTDIGVYRSVRTGTAHSVWTLHSTGLPESAVMDLQVH
                     AASRTLRAATHGRGVWELPLDFVAPPHPELFLRMNVGDRGRRLAAADGGAHPDTPGAV
                     LGRAASPDIRVRRGVAAAAPPPFPGRLLRQVRPVRMKGEDIRTWQQGVADRGFALGVD
                     GTYGPESDRVCRRFQARFGLVVDGIVGPITWPATFAYPRLADATSALAVELGTVDDVD
                     PVSGATRADSAGTNRLFVEVHSRSVHPVDPGEVVVCLLFADDAGAGAPALPNGYANRI
                     RARDTGAWLAGSGWVFANPGAPYAPLPRPLSASSPQVVEYLVDFSTLALPSKNVLALA
                     LVSTADGTDPLTAPETAPLTLVRTERRAAAKRLLLVP"
     gene            complement(3759..7538)
                     /locus_tag="VEO00_05765"
     CDS             complement(3759..7538)
                     /locus_tag="VEO00_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57540.1"
                     /translation="MPIDRDSPLLGPEFSATARSGLSAATAATWSPTLPRDPRLLVPV
                     DVEALIIPPGAAPEAHADIAGRLLRAPDAPGETFADDTAPAPPPFTDAPARPAGAYLH
                     WAVPDGLTAGRVTDGTDPDGRLNLAPLPDRWLVVRLGDERPRTLTAWVIEADRKRRVR
                     LADWREDPDGTEGETPTLDPAGLTAVSGGDPAWAAVFDNVEDRFAMYDDLAGVGAGAT
                     VSYVVVGWYSRAELDPLRGAGDLASFTRRLAELHWSVDEARLAAAFARSRARADAAGA
                     RKLATPPPQTGPFTAEAEVEGRSVPVPGPAGSPKIAAAGARILVHPAPSGPRQSLFHG
                     TVHGVRVDGAGGDARPRTEDLTLAVGATSVEAMAASLRSRLQSDAATERLLCAFGYRV
                     LGALGRPDGLVAFDEEVHRRAFVSLPGGSVEERIRMGDALAGLRPPPPPAPGTRGRGG
                     GRDVEAERPGFEALARDPRVPASAVEEAAKRLVGGRVRFDLVERDPASVAGIFTGASV
                     PSRVPSADPPRIVTVRRALPRWYFPADPVVVAIGLNRSLRHGHDGRLEPDETLACRLS
                     DEIATGYQGLLAGADLVSGSLEHGGVPDEADELVREAALRDWGQIDAMIDTAARGRGL
                     PRAPVAARMQAERDLALHGSLPGRDSALLLPGSLIDGRIPSAMWVTYWRQPWVPLYLE
                     WELELRVDDRLDRWTLGEVDLEPAGEPAAGAPARTVRGRALLSSGATKALVQDITEFL
                     AQETLRDQEGTGIVSDLDAALLRDLGERAGRLDALGAALEGIREFLLGFDTNAGLSGP
                     DDEHVEPAPSRDPELVRGGTATLARLRVVDAFGRTLELDPAALQRVTLSDQLEAGPAT
                     LLLPPRVTAPSRLLLRFVDPTDEEVEARVDQEDPARAVSPVAGWLLPDHVDGALELFD
                     AEGEPAGQLLHEGLGGAVVYEGAPGVPGPAGVAPRTDRPGDRHLAAFAVAVARRDAED
                     RKLPAEARPGESALAALLRVIDTTLWTVDPFGSAGVEHLSVLTGRPLALVRARLELEV
                     RSDVDELPLDPAAAARRQARFDALAATAFPVRLGALTRGDDGLLGYFLDDDYRVFHPL
                     HDGVLRDALPAGPQQGFLGSPAEVGAFAGDLGSHTAPIRHPFVQGRPELLLRAGQRRT
                     LTLLVVPGGAVHLTSGISPRKDVALLRSWTAEPLGRIAPSFRVGPVLVDPETIRMPRV
                     STFDPEQVFTRRTGPESWRDDPIVAASQQALLPDTPAEAQEGYLRVRIEQEDGPS"
     gene            complement(7549..8454)
                     /locus_tag="VEO00_05770"
     CDS             complement(7549..8454)
                     /locus_tag="VEO00_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57541.1"
                     /translation="MNVLIDPGRILTEAYATATAPAPVQGEDQELPGQVARFLAHLRL
                     LEDVPFQYLVPDSALLPPESIRFFYLDRNWSDAAVDGALAAGTFTSAERATLTARHAE
                     IRAAVDRAERDVWADRLGSAGHDGPAEVVTGFLLRSRAVSGWPGMHVRAYREGNAAPM
                     RMLRIERLAPAVLFALIDGIPDSVVLEEPRQGIQFGVDADPSGRFHANVRDPADGSFT
                     GTTAPVPFRRDSPGLIDMTTLRARLVATGEVGSALDAAEYALQMLQLPYQQWFGEGGG
                     PVTMRDFLHTVIAVGEMRAWLRTDG"
     gene            complement(8451..10277)
                     /locus_tag="VEO00_05775"
     CDS             complement(8451..10277)
                     /locus_tag="VEO00_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57542.1"
                     /translation="MAQNRVEVFPAMIPPLTAGRYELRLGQRMAAAGGTNPLGDVAEE
                     VRHVEVTAPRVAMSGTELFSTYPPPNASGPFSTRLPQVAFRRRTLPWERAMPAPAGGS
                     GATLNPWLALVTVTAQEATFLTGLKAPDAYTPAVAASLGVVDDGSRADAIELTRTGVH
                     QLFPRKDELHLLAHVRKVDVHDSELAGNDDDGWVAVLLSNRLPQPGIAYRVCVVSLEG
                     QFAALADPQAAVVDTPSKVHLYADLDAAVLEAATFSAAGEPVPLGATDQPTPDAAGRL
                     TGARARPPDGWVQAPGAVRAGRGGGAPVTARDIAVAAFGGGFVRPELDLRLVEPAVPR
                     FRFPVLASWEFVCEGSGDFESLMQGLDVGLLGTVPAAGEAAQGPAHLVLETGHLELDH
                     RRRRGVSGRAWYRGPLTPREVARRPATVPFAVADQARRVATDGLEDLSEAAAFEIGRL
                     LALAAPRFVAALRTWAREGFVVRQTRSRLSGLGVLGRPELELAPSLAALGLLSRLADD
                     EARVLGPQVPVVDLGPMAGRAERLDAGSLAAGLGLDAAFVEDVLAPGPTTAGLRDVPA
                     AALLDDADRLAQDPATLAHLGEALHEAVGRLAGGAGIGRGNG"
BASE COUNT         1300 a         3833 c         4016 g         1195 t
ORIGIN      
        1 gctcacgcag gaacggcgat cccggaaccg ctggccggct gagaacgtcg acgccaaccg
       61 cgccgcccag tccgaaaccc agccgaccac agcgcaaacg agagaagacc ccgctccgag
      121 gctttgcgca acagcctcaa agcaccggcc ttttgctttc tcatttcctt gcacttctcc
      181 cagtacgccc aggtatggaa caggttttgg aacagccaga ttcagaacgg gccggttttg
      241 tcgtctggct tggcaacgga gggatcgggc gccagacgcg ctcccatcgg ttgacctagc
      301 tctctgagag aaacgaggcc gtcgcgcacg ccctcggtga cacggcacgc ctccggtcgc
      361 ctcgggcgct ccgcctgctc gtggtcaggg cacgagcagt aagcgcttcg ccgccgcgcg
      421 ccgctcggtg cgcaccagcg tcagcggggc cgtctcgggc gcggtcaggg gatccgtccc
      481 gtcggcggtc gagaccaggg ccagcgctag gacgttcttg gacggcagcg cgagcgtgct
      541 gaagtcgact aggtactcga cgacctgggg gctcgatgcg gagagcggcc gcggcagcgg
      601 ggcgtacggc gcgcccgggt tcgcgaagac ccagccgctg cccgctagcc acgcgcccgt
      661 gtcccgggcg cggatccggt tcgcgtagcc gttgggcagc gcgggggcgc ccgccccggc
      721 gtcgtcggcg aacaggaggc agacgacgac ctcgccggga tcgaccgggt gaacgctgcg
      781 gctgtgcacc tccacgaaca ggcggttggt gcccgcggag tccgcgcgcg tcgcgccgct
      841 caccgggtcg acgtcgtcga ccgtccccaa ctccacggcc agcgcgctcg tggcgtcggc
      901 gagcctcggg tacgcgaacg tcgccggcca cgtgatgggg ccgacgatgc cgtcgacgac
      961 caggccgaac cgggcctgga agcggcggca gacgcggtcg gactccggtc cgtaggtgcc
     1021 gtcgacgccc agcgcgaagc cccggtcagc cacgccctgc tgccaggtgc ggatgtcctc
     1081 gcccttcatg cggaccggcc gcacctgccg cagtagccgc ccagggaaag gcggcggcgc
     1141 ggcggcggcc acgccgcgcc ggacgcggat gtcggggctc gccgcgcgtc cgagtacggc
     1201 ccctggcgtg tcggggtgcg cgccgccgtc ggcggccgcg agccggcgcc cgcggtcgcc
     1261 cacgttcatc cgcaggaaga gctcggggtg gggcggcgcg acgaagtcga ggggcagctc
     1321 ccacaccccg cgcccatgcg tcgctgcacg cagggtgcgg gatgcggcgt ggacctgcag
     1381 gtccatgacg gcggactcgg gcaggcccgt ggagtggaga gtccagacgc tgtgcgccgt
     1441 tccggtccgg acggagcggt agacgccgat gtccgtgccg gcgaacaccg tcgtcgggtt
     1501 ggccgggtcg atggccacgc aggaggccgg aatgtcgacg acctggggcg tcccggcccc
     1561 gccgccgggc atgagcatga gaccggtggc gtgccaggcc gcgccgtcga agtaccaaca
     1621 gtggtcgacg ccgagaccgc cgaccgtcgc gtagagcgtc gtcccgcccg cggaggcgat
     1681 cgacgtgatc ggggtcgccg ccgcgacgcc tgggatcgcg ggcagcgtgg tccggctcca
     1741 cacccccgcg gcgaggtcga accggaacac gctcgtggcg gtggcggcga agagcaggac
     1801 ggcggacggg aaggccaggc cgcggacgcg cccggcaagc gtgtcctgga ccaggctggg
     1861 gacggccgcg gcgtacgggt tcgttcccgt cggcagtgtc acccacgacg tgccccagtt
     1921 gctcgacagc cacacgcggt gggtgccctt ggcgacgagg gtcggcacga cgccggcggg
     1981 cgtggtggcg atcggcgagt agaagtcgga gttcgccccg cccccctcgg tggccgccgc
     2041 gttgccctgc gcgaccgtgt tcgccgtccg gggcgggaag ttcagcgcgg tccagttcgc
     2101 ccgggtggcg ccgcccgtgg cggtctgcga cagcgacgcc cggatgtact gccgcatcat
     2161 gcggtagggg ttgttgggat cgatcgcgac gccaccgccg tcggcgacga gcgtctcgtg
     2221 ccacgtcggg ccgctcgtcc agcggatggt gccgttgtcc tggcagccgg cgagggcgac
     2281 ggcctcgagc gtggggtggg cggccaggta ggtcatctgc gcggtggaca gtccagcccc
     2341 gcgcgaggtg aaggtgccga gcgcgcccgc cgcggcggag tggaagatcc cgccgtcgca
     2401 gccgatccag acgccggcgc cgtcgagccc ggtgttgtcg gcgcgccgcg agaaggcgat
     2461 ggactgggcg tcggcgtgga tcccgcggcc gacgaacgtg ggatcggtgc tctcgcggaa
     2521 cacgcccgcg gtgcccggcc cgttgttggc ggcgttgaac gggaaggcgg gcgtcaccgc
     2581 tgggttggtc accgtcccgc ggaagagcgc gagcgtccag tccgcgacgg gatcgcccgg
     2641 cgcgaggtcc cacgccaccg agccgccgag gtaaaccgtg tcggccgcgg cggggtcgac
     2701 cgcgaggatg atgttgtacc agccggtccc ggtcgtcccg aagacgttgg tcggcacgcc
     2761 ggcgacggcc gcgaagctgc cggcggtgag ccggaacagc cggcgcgact ggcagaacgc
     2821 gtagaccacc gagggcgtga cctccgacgc tgcgagcacg atgcgcccgt cgccggccgc
     2881 gggcccgccg cctaccgcgg ccgcgggcac gcggctccac gccgcgaggt cggggctcga
     2941 gtagacgccg tcgttctcga acgccgcgta gtaggtcttg gccgcgcccg ctcccgcgat
     3001 cagcacgtcg ctcaccacgc cgaggccgtt ggtgaaggcc gggctggcga cctgcgtcca
     3061 cgttgcgcgc gcggcggccg cggcggggcg ggtgatgagc ccgcgggtgg tggccacgac
     3121 aacgagggcc gggtcgtccg ggtcgatgac gagcttcgtg cagccgaggc cggcgaggtt
     3181 cgtcccctcg agcgaccacg acccgagctg gccgcccgcg gtggagacgc gcacgccgat
     3241 gccgaacagc ccccggtagc cggcgcggta ctccccggtg ccgacgaaca cctggtcgcc
     3301 ggcgcggtcg gcgccgaagg ccacggccag ggcgccgacg gagagcgagt tcgccacgcg
     3361 gccgggcgtc ccgggcgccg tggtggcgaa gtcgtcgagt acctcccagg tggcgccccc
     3421 gtcgggggag aaccaggcgc cgccgtcagc ggcgccgatg taaacgcggg tgccgccggg
     3481 gccggccgcg atcgcctcga tgcgccccga gacgaccggg tgccccgacg cctggccgcc
     3541 ggtgatggcg aacgggccga gcgggatcca gttcacggcg ccgccgggcg cggccggggc
     3601 ggcgggcgcg gccgcggcgg ccggcgcgaa ggcggcctca cgtcgggcgg tcgccgcgcg
     3661 gaggaacgcc tcgccgggca gtccccgccg gccgccgcgg gagcggtgga accaggcgga
     3721 cctcccgccg gggtcgtcat gctggcgcgg cccgctcgtc acgacgggcc gtcctcctgc
     3781 tcgatgcgca cgcgcaggta gccctcctgg gcctcggccg gcgtgtcggg cagcagcgcc
     3841 tgctggctcg ccgcgacaat cgggtcgtcc cgccaggact ccggcccggt acgccgggtg
     3901 aacacctgct cgggatcgaa ggtggacacc cgcggcatcc ggatcgtctc cgggtcgacc
     3961 agcaccggcc cgacccggaa cgacggcgcg atccggccga gcggctccgc ggtccacgag
     4021 cgcaggaggg cgacgtcctt gcgcggcgag atccccgagg ttaggtgcac ggcgccgccg
     4081 gggacgacca gcaaggtcag cgtccgccgc tgcccggcgc gcaggagcag ctctgggcgg
     4141 ccctgcacga acgggtgccg gatcggcgcg gtgtggctgc cgaggtcgcc ggcgaaggcg
     4201 ccgacctcgg ccggggagcc gaggaacccc tgctgcggcc cggcggggag cgcgtccctc
     4261 agcaccccgt cgtggagggg atggaacacc cggtagtcgt cgtccaggaa gtagccgagc
     4321 aggccgtcgt cgcctcgggt gagcgcgccg aggcggacgg ggaaggccgt cgcggcgagc
     4381 gcgtcgaagc gcgcctggcg ccgcgccgcc gccgcggggt cgagcggcag ctcgtctacg
     4441 tcggagcgga cctcgagctc gaggcgtgcg cgcaccaggg ccaacgggcg ccccgtaagg
     4501 acggacaggt gctcgacgcc ggccgagccg aagggatcca cggtccagag cgtcgtatcg
     4561 attacgcgca gcagggcggc gagcgcggac tcgcccgggc gcgcctccgc cggcagcttt
     4621 cggtcctcgg cgtcgcggcg cgcgacggcc accgcgaacg ccgccagatg ccggtcgccg
     4681 gggcggtcgg tgcgcggtgc cacgccagcg ggcccgggca cgccaggcgc gccctcgtag
     4741 accaccgcgc cgccgaggcc ctcgtgtaac agctgcccgg cgggctctcc ctcagcgtcg
     4801 aagagctcga gcgcgccgtc gacgtggtcg ggcagcagcc accctgcgac tgggctgacc
     4861 gcccgggcgg gatcctcctg gtcgacgcgc gcctcgacct cctcgtcggt cgggtcgaca
     4921 aagcgcagca gcaggcggct cggcgcggtg actcgcggcg gcagcagcag ggtggccggc
     4981 cccgcctcta gctggtcgga cagcgtcacg cgctgcagcg cggcggggtc gagctcgagt
     5041 gtgcgcccga atgcgtccac gactcgcagt cgggcgagcg tggccgtgcc gccgcgcacg
     5101 agctcggggt cgcggctcgg cgccggctcg acgtgctcgt cgtcgggacc cgacaacccg
     5161 gcgttcgtat cgaagccgag caggaactcg cggatgccct ccagggcggc gcccagcgcg
     5221 tcgagccggc cggcccgctc gccgaggtcg cgcagcagcg cggcgtccag gtccgagacg
     5281 atgccggtgc cctcctggtc gcggagcgtc tcctgggcga ggaactccgt gatgtcctgc
     5341 accagggcct tggtcgcgcc cgagctcagc agtgcgcgcc cgcgcacggt gcgcgcgggc
     5401 gcgcccgccg ccggctcccc ggccggctcg aggtcgacct cccccagcgt ccagcggtcc
     5461 agccggtcgt ccacgcggag ctcgagctcc cactcgaggt acagcgggac ccatggctgg
     5521 cgccagtagg tgacccacat cgccgagggg atccggccgt cgatgaggga gccgggaagg
     5581 agcagggcgc tgtcgcgccc gggcagcgac ccgtgcagcg cgaggtcgcg ctcggcctgc
     5641 atccgggccg cgaccggcgc cctgggcagt ccccggccgc gggcggccgt gtcgatcatt
     5701 gcatcgatct gtccccagtc gcgcagggcc gcctcgcgca cgagctcgtc ggcctcgtcc
     5761 gggacgccgc cgtgctcgag gctgcccgag acgaggtcgg cgccggcgag caggccctgg
     5821 tagcccgtcg cgatctcgtc cgagagccgg caggcgagcg tctcgtcggg ctcgagccgg
     5881 ccgtcgtgcc cgtgccgcag cgaccggttg aggccgatcg ccacgacgac cgggtccgcg
     5941 gggaagtacc agcgcggcag cgcgcgccgc acggtgacga tgcgcggcgg atcggcggac
     6001 gggacccggc tcgggacgga ggcgcccgtg aagatcccgg cgaccgaagc cgggtcgcgc
     6061 tcgaccaggt cgaaccgcac gcggccgccc acgagccgtt tggcggcctc ctccaccgcc
     6121 gaggcgggca ccctggggtc gcgcgcgagc gcctcgaagc cgggacgctc ggcctcgacg
     6181 tcgcgcccgc cgccccgtcc cctcgttccg ggcgcgggcg gcgggggcgg ccgcaggccg
     6241 gcgagcgcgt cccccatgcg gatgcgctcc tcgacggagc cgcccgggag gctcacgaag
     6301 gcgcgccggt ggacctcctc gtcgaaggcc acgaggccgt ccggccgccc gagcgcgccg
     6361 agcacccggt agccgaacgc gcacaggagc cgctccgtcg ccgcgtcgct ctgcaggcgc
     6421 gagcgcagcg acgccgccat cgcctcgacg ctcgtggcgc ccacggctag cgtgaggtcc
     6481 tccgtccgcg ggcgcgcgtc gccgccggcg ccatcgaccc tgacgccgtg cacggtgccg
     6541 tggaagagcg actggcgcgg cccggacggc gccgggtgga cgaggatccg ggcgcccgcg
     6601 gcggcgatct tcggcgaccc ggccggtccc ggcaccggga cggaacggcc ctcgacctcc
     6661 gcctccgccg tgaacgggcc ggtctggggc ggcggggtgg cgagcttgcg ggctcccgcc
     6721 gcgtccgctc gggcccggga gcgcgcgaag gccgcggcaa ggcgtgcctc gtcgacgctc
     6781 cagtgcagct ccgccagccg gcgggtgaac gacgcgaggt cgcccgctcc gcgcagcggg
     6841 tcgagctcgg cgcgcgagta ccagccgacc acgacgtagc tgaccgtcgc gccggcgccg
     6901 acgcccgcga ggtcgtcgta catggcgaac cggtcctcga cgttgtcgaa gacggccgcc
     6961 caggccgggt cgccgccgct cacggcggtc aggccggccg gatcgagcgt gggggtctcg
     7021 ccctcggtgc cgtcggggtc ctcgcgccag tccgcgagcc ggacgcggcg cttccggtcg
     7081 gcttcgatca cccacgccgt cagcgtccgc ggccgctcgt cgccgagccg gacgacgagc
     7141 cagcggtcgg gcagcggcgc gaggttgagc cggccgtcgg ggtccgtccc gtccgtgacc
     7201 cggccggccg tgagcccgtc cggcaccgcc cagtgcaggt acgccccggc cgggcgggcg
     7261 ggggcgtccg tgaacggcgg cggcgccggc gccgtgtcgt ccgcgaacgt ctcccccggc
     7321 gcgtccggag cccgcagcag ccgtcccgcg atgtcggcgt gcgcctcggg cgcggcgccc
     7381 ggcggaatga tcagcgcctc gacgtcgacc gggacgagga gccgcgggtc ccgcggcagc
     7441 gtaggggacc acgtggcggc ggtggcggcg ctcaggccgc tgcgcgccgt ggccgagaac
     7501 tccgggccga ggagggggga gtcgcggtcg atcggcatcg cggccgcctc agccgtccgt
     7561 ccgcagccac gcgcgcatct cgccgaccgc gatcaccgtg tgcaggaagt cccgcatggt
     7621 gaccgggccg ccgccctcgc cgaaccactg ctggtagggc agctggagca tctgcagggc
     7681 gtactcggcg gcgtcgagcg cgctgccgac ctcacccgtg gccaccagcc gggcgcgcag
     7741 ggtggtcatg tcgatcaacc cgggggagtc ccggcggaac gggaccggcg ccgtcgtccc
     7801 cgtgaagctc ccgtccgcgg ggtcgcggac gttcgcgtgg aagcgaccgc tcggatcggc
     7861 gtcgacgccg aactggatgc cctggcgcgg ctcctccagg accacgctgt cggggatccc
     7921 gtcgatcagc gcgaagagca ccgcgggcgc cagccgttcg atccgcagca tccgcatggg
     7981 cgccgcgttc ccctcccggt aggcgcgcac gtgcatcccc ggccaccccg agacggcgcg
     8041 gctgcggagc aggaagcccg tgaccacctc ggccgggccg tcgtggcccg cgctcccgag
     8101 ccggtcggcc cagacgtcgc gctcggcgcg gtcgaccgcc gcgcggatct cggcgtgccg
     8161 cgccgtgagc gtggcgcgct ccgcggaggt gaacgtgcca gccgccagcg cgccgtccac
     8221 cgcggcgtcg ctccagttgc ggtcgaggta gaagaatcgg attgactcgg gcggtaggag
     8281 cgcgctgtcg ggcacgaggt actggaacgg cacgtcctcc agcagccgca ggtgggcgag
     8341 gaagcgggcc acctggccgg gcagttcctg gtcctccccc tgaaccggcg cgggggcggt
     8401 cgcggtggcg taggcctccg tgaggatccg gcccggatcg atgagtacat tcatccgttc
     8461 cccctcccga tcccggcgcc gccggcgagc cggccgacgg cctcgtgcag cgcctcgccg
     8521 aggtgggcga gggtggcggg gtcctgcgcc agccggtcgg catcgtccag cagcgccgcc
     8581 gccggcacgt cgcggagccc ggccgtggtg gggcccggag cgaggacgtc ctcgacgaag
     8641 gcggcgtcca gccccaggcc ggcggccagc gagcccgcgt cgagccgctc ggcccggccc
     8701 gccatcggac ccaggtcgac gaccggcacc tgcggcccga ggacgcgcgc ctcatcgtcc
     8761 gccagccggc tcagcaggcc gagggcggcg agggacggcg ccagctcgag ctcggggcgc
     8821 ccgaggacgc ccaggccgga gagccggctg cgcgtctgcc gcaccacgaa cccctcgcgc
     8881 gcccaggttc gcagcgccgc gacgaagcgc ggcgctgcga gcgcgagcag gcgcccgatc
     8941 tcgaaggcgg cggcctcgga gaggtcctcc agcccgtccg tcgccacccg ccgcgcctgg
     9001 tcggcgaccg cgaagggcac ggtggccggg cgccgggcca cctcgcgcgg cgtcagcggc
     9061 ccgcggtacc aggcgcggcc ggacaccccg cggcgccggc gatggtcgag ctccaggtgc
     9121 cccgtctcga ggacgaggtg ggccgggccc tgcgcggcct cgcccgcggc cggcaccgtc
     9181 ccgagcagcc cgacgtcgag gccctgcatg agcgactcga agtcgccgct gccctcgcag
     9241 acgaactccc agctagcgag gacggggaag cggaaccgcg gcacggcggg ctcgaccagg
     9301 cgcaggtcga gctcgggccg cacgaagccg ccgccgaatg ccgcgacggc gatgtcgcgc
     9361 gccgtcacag gcgcgccgcc gccgcgcccg gcgcgcaccg cgccgggcgc ctggacccac
     9421 ccgtcgggtg gcctcgcgcg cgcgcccgtg agccggccgg cggcgtccgg ggtcggctgg
     9481 tccgtcgcgc ccagggggac gggctcgccc gcggcgctga acgtcgccgc ctccagcacc
     9541 gcggcgtcga ggtcggcgta gaggtggacc ttggacggcg tgtcgaccac ggcggcctgg
     9601 ggatcggcca gcgccgcgaa ctgcccctcg agcgagacca cgcagacccg gtaggcgatc
     9661 ccgggctgcg gcagccggtt cgacagcagc actgccaccc agccgtcgtc gtcgttgccg
     9721 gcgagttcgc tgtcgtggac gtcgaccttg cggacgtgcg cgagcaggtg cagttcgtcc
     9781 ttgcgaggga agagctggtg gacgccggtg cgggtgagct cgatggcgtc cgcccggctg
     9841 ccgtcgtcga ccacgcccag gctcgccgcg accgcgggcg tgtaggcgtc cggcgccttg
     9901 aggccggtca ggaaggtggc ctcctgcgcg gtgaccgtca cgagcgcgag ccacgggttc
     9961 agcgtcgcgc cggagccgcc ggcgggcgcc ggcatcgcgc gctcccaggg gagggtccgg
    10021 cgccggaacg cgacctgcgg caggcgcgtg gagaagggcc ctgaggcgtt gggcggtggg
    10081 taggtgctga acagctcggt gccgctcatc gcgacgcgcg gcgccgtgac ctcgacgtgg
    10141 cggacctcct ccgcgacgtc gccgaggggg ttcgtcccgc cggcggcggc catccgctgg
    10201 ccgagccgca gctcgtagcg gccggccgtc agcggcggga tcatcgctgg gaacacctcg
    10261 acccggttct gcgccatcgc tctcccctca ctcgctccag gccaccgcgg acgagcccgt
    10321 cgggccgccc acgagctccg ccac
//
LOCUS       DASPWO010000257         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_38406, whole genome shotgun sequence.
ACCESSION   DASPWO010000257 DASPWO010000000
VERSION     DASPWO010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38406"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2097)
                     /locus_tag="VEO00_05780"
     CDS             complement(<1..2097)
                     /locus_tag="VEO00_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57543.1"
                     /translation="MREALWEAIDDAKGDDPLAPVTVAAPSAYAGLSLRRLLAARPPG
                     RAGGRPGLVNVRFLPLARVAELLGAPALAAAGRRPLTAPLRTEAVRAVLAVEPGAFAG
                     VASHPATVRSLESSFRDLRRAPLATVRALEARPDPAASVARLFGAYRTRTSSFYDEED
                     LAEAAAIAAGSGTPALEELGHLVVHLPSRLSPGEGALLEALAARGRLSAVLGRTGDDA
                     DEPVGAAVLDRFGAIVGPPSSTGVAPDPPTGTTIISAPDPEDEVRAVLRSVIERAARG
                     VPLHRLAVLYREADPYARLVRELFEAAGVAWTGPATRRLADSVAGRVLLGMLGLADRG
                     FRRDEVAAWVASGPVLDPATGHPVPAARWDTLSREAGIVDGLDQWRARLERHARACTT
                     DLAAAQAEDTAPESLVARLESDIEQVARLAAFVDDLGARLTPPEPADWPTLTAWAREL
                     LRVYLGGEGRRGRWPEDELDAARRVDAALDGLGVLAEIRPGTDPVTFLRAVEAELDAP
                     IARTGRFGRGVLVGPLRHAYGGDFDALFMLGMAEGSFPPRTRDDPVLPDRDREAVTTD
                     ALPLHALRRAEERRDYLAALAAAPERILCFPRANPRAQRKRLPARWLVESAAALAGRS
                     IGAEELVGLANAPWLQVVPSFEGGMCGDGDAGSVGEHDVRSLRSWQLAGHRTVRHPLV
                     ASQPDLRAGVTATDERA"
BASE COUNT          276 a          773 c          855 g          214 t
ORIGIN      
        1 cgcccgctca tcggtcgcgg tgacgccggc ccggaggtcg ggctggctcg cgacgagggg
       61 atgacggacg gtgcggtggc cggcgagttg ccagctgcgc aggctgcgca cgtcgtgctc
      121 gccgaccgat ccggcgtcgc cgtcgccgca catgcccccc tcgaaggacg gcacgacctg
      181 cagccacggc gcgttcgcca accccaccag ctcctcggca ccgatggagc ggccagccag
      241 cgcggcggcc gattcgacga gccagcgggc gggcagccgc ttgcgctggg cgcggggatt
      301 ggcgcgcggg aagcacagga ttcgttccgg ggccgctgcg agcgcggcga ggtagtcgcg
      361 gcgttcctcg gcgcgccgca gcgcgtgcag cgggagcgcg tcggtggtca ccgcctcgcg
      421 gtcgcggtcg ggcagcacgg ggtcgtcacg ggtgcgaggc gggaacgagc cttcggccat
      481 gccgagcatg aacaacgcgt cgaaatcgcc gccgtacgcg tgccggagcg ggccgacgag
      541 caccccacga ccgaagcgcc ccgtccgggc gatcggcgcg tcgagttcgg cctcgaccgc
      601 ccgcaggaag gtgacgggat cggtgcccgg acggatctcg gcgagaacac cgagcccgtc
      661 gagcgcggcg tcgacccggc gggccgcgtc gagctcgtct tcgggccaac gaccgcgacg
      721 tccctcgcca ccgaggtaga cccgcagcag ctcgcgcgcc cacgcggtca gggtcggcca
      781 gtcggcgggc tcgggcggcg tcaggcgggc gccgaggtcg tcgacgaacg ccgccagtcg
      841 cgcgacctgc tcgatgtcgg actcgagccg cgccaccagc gactcggggg cggtgtcctc
      901 ggcctgggcg gcggccaggt cggtcgtgca ggcgcgggca tggcgctcga ggcgggcgcg
      961 ccactggtcg agcccgtcga cgatgccggc ctcgcgcgag agcgtgtccc agcgggcggc
     1021 cggtaccggg tggccggtgg cggggtcgag caccggaccc gacgccaccc atgctgccac
     1081 ctcgtcgcgg cggaaccccc ggtcggcgag cccgagcatg ccaagcagga cgcgcccggc
     1141 gacgctgtcg gcgagccggc gcgtcgccgg gcccgtccag gcgacgccgg cggcctcgaa
     1201 cagctcgcgg acgaggcggg cgtacggatc ggcctcgcga tacagcaccg ccaagcggtg
     1261 caggggcacg ccgcgtgccg cccgctcgat gaccgaacgc agcaccgccc gcacctcgtc
     1321 ttcggggtcg ggggcgctga tgatcgtggt gccggtgggc gggtcggggg ccaccccggt
     1381 cgacgacggc ggcccgacga tcgcgccgaa ccggtcgagc accgcggcgc cgacgggctc
     1441 gtcggcgtcg tcgccggtgc ggccgagcac cgcgctgagc cgcccccgtg cggcgagcgc
     1501 ctcgagcagc gcgccctcgc cgggcgacag gcgcgaggga aggtggacga cgagatgccc
     1561 gagctcctcg agcgccggtg tgcccgagcc cgccgcgatc gcggcggcct cggcgaggtc
     1621 ctcctcgtcg tagaaggagg aggtgcgggt gcggtaggcg ccgaacaggc gggccaccga
     1681 cgcggcggga tcgggtcggg cttcgagcgc ccggacggtg gcgagcggag cccggcggag
     1741 gtcacggaag gacgattcga ggctgcgcac ggtcgcggga tggctggcga cgccggcgaa
     1801 cgcgcccggc tcgaccgcca ggaccgcccg caccgcctcc gtccgcagcg gcgcggtgag
     1861 cgggcgccgg cccgcggccg ccagcgcggg cgcgccgagc aactcggcga cgcgggcgag
     1921 gggcaggaat cggacgttga ccagccccgg acgcccccca gctcgacctg ggggccgcgc
     1981 cgccagcagc cgtcgcagcg agaggccggc gtaggccgag ggagcggcga cggtcacggg
     2041 ggcgaggggg tcgtcgccct tggcgtcgtc gatcgcctcc cacagcgcct cccgcaccgg
     2101 ggaaccatgg gcacagag
//
LOCUS       DASPWO010000258         6420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_294908, whole genome shotgun sequence.
ACCESSION   DASPWO010000258 DASPWO010000000
VERSION     DASPWO010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294908"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2965
                     /locus_tag="VEO00_05785"
     CDS             <1..2965
                     /locus_tag="VEO00_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57544.1"
                     /translation="GVDPALFQDLRWRLIGPFRGGRVLAVAGVPGEREHFYFGSVNGG
                     VWETVDAGRTWQPIFDGQPIGAIGAIALAPSDPKVIYVGSGEADMRSDIAQGDGMYKS
                     ADAGKTWTKIGLPDTQQIGRILVDPSDPGRVLVAALGHPYGPNAERGVFRSPDGGAAW
                     RKVLFKDENTGAIDLAFEPGDSRVVYAALWQTRRPPWSVYPPSNGPGSGLYKSTDGGD
                     TWRPLVGHGFPARPGRIGLALSPSRPERVYAMVDAPDGGLYRSDDRGASWTRASGDRR
                     IWGRGWYFGGITVEPRDPDVVYACNTSLYRSRDGGRTFVPIQGAPGGDDYHELWIDAD
                     HPERRMLGADQGAVVSLNGGRTWSSWYNQPTGQMYHVSTDDRFPYWVYGSQQDSGAAA
                     VPSRTTTHDGITMMQFREMTAGSESDVIAPDPKDPDVIYGGRVDRLDLRTQQTQSIDP
                     TLLHRDVDRATWTLPLAFSRRDPRVLYFSRERLFRTMDGGRHWTVISPDLTREDPGVP
                     ATLDPAAAADAPRPGRRHGVIYAIAPSRLADHDLWIGTDDGLVWRTRDEGAHWENVTP
                     AGLGPWSKVGIIDPSHFDGESAYAAVDRHRLDDSRPYVYRTHDGGRTWTLAATGIPAG
                     SFVNAVREDPVRRGLLYAGTEKGVCVSFDDGDHWQPLQLNLPVTSVRDIDVHGDDLVI
                     ATHGRAFWVLDDVTPLRQAGPDVASAPAWLFAPATAIRLRPAGFTGTPLPRDEPTAAN
                     PPPGAVIDYVLSKAGTAPVVLRILDARGALVRRCSSDEGPPAPDLAKIRVAPEWMTPP
                     VALTTKPGMHRFVWPIRYPAPPALSEHNLYADGVWAPPGRYTVALTVDRQRLTQPLTI
                     APDPRVALPPEAYARQLALARRVEALRESIAAALAEAEKLHVDLAAKGASDLDARVRA
                     LTGPDFGEAATAGPPAGLSSLRALASSLATLANAVDGADAEPTPDAEAAIPKVEQAVQ
                     ATLAAWATLKQQWSP"
     gene            complement(3169..4449)
                     /locus_tag="VEO00_05790"
     CDS             complement(3169..4449)
                     /locus_tag="VEO00_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYU57545.1"
                     /translation="MPLDRRRFLVATGMSVAATALARPASTASPQDWAWVRDQFDLRR
                     DLLHFASFYLVSHPRPVREAIETLRRALDENPFEVVDHGLFTRPDEVRRAAAVYLGGR
                     PEDVALTRSTTEGLALIYAGLRLRSGQEILTTAHDHYVHHESIRLAAQRDGAEMRRVA
                     LYDEGSRASEDEIVDRLRRAVRPGTRVIGVTWVHSSTGVKLPIRSIAASVAELNRSRD
                     PADRVLLVVDGVHGFGVEDESAADLGCDFLAAGAHKWIFGPRGTAIVWGRREAWERVQ
                     PSIPAFEMPPYMAWQHGVEPGPTQGAWMSPGGFHAYEHMWALPAAFAFHREIGRARIA
                     GRIHEMNTRLKDGLAGLRHVTLHTPRAPGLSAGLVAFEVAGVTPEEVVRRLRERRIIA
                     STSPYLQSFARLAGSILNTPEEVDSAVAAVGALA"
     gene            4578..5000
                     /locus_tag="VEO00_05795"
     CDS             4578..5000
                     /locus_tag="VEO00_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HYU57546.1"
                     /translation="MFANSHFALGTHVLVSLALQEGSPVTSAELARSINTNPAFLRML
                     LGRLREAGLIEVALGKGGGATLARPASKLTLADVYHAVERQPPARLHRCPPSRSCIVG
                     RNILPVLQAVVHDVESAALGRLMGITVAKLADQVRRRG"
     gene            5105..5854
                     /locus_tag="VEO00_05800"
     CDS             5105..5854
                     /locus_tag="VEO00_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYU57547.1"
                     /translation="MRRLENKIALITGGTTGIGLATAKRFAEEGAQVIVTGRNPATLE
                     LARRELNGRVQVLESDAADERQVAELFESVGREHGRLDVLFLNAGIAKFAPLAEAPLS
                     DFDAMWHLNVRGPWLALKHALPILTEGAAVIVNTSVVNQKGMAGTTAYAATKAALRVI
                     VRAAAAELAGRKVRVNAISPGPIETPIFEKTGLSAQEREAFRKDVAARVPLGRFGAAH
                     EVASAAAFLASPDASFITGTELAVDGGFAQV"
     gene            complement(5984..>6420)
                     /locus_tag="VEO00_05805"
     CDS             complement(5984..>6420)
                     /locus_tag="VEO00_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57548.1"
                     /translation="KLSGIFQARSGVPMTLQDSRGSSLQAVRGVERPNCIGDPKPADQ
                     TIDHWLDINAFSRAPLGTWGTCGVGTARAPGYRNIDAVLSKRFDMGGERYLEVRAEAF
                     NLTNTPSFTFPGRNIADVNTFGKITNTVSLPRNVELVVKFFF"
BASE COUNT          936 a         2362 c         2262 g          860 t
ORIGIN      
        1 cggcgtcgac cccgctctct tccaggacct gcgctggcgg ctcatcggac ccttccgcgg
       61 cggccgggtg ctggcggtcg caggggtgcc cggcgagcgc gagcatttct acttcggcag
      121 cgtgaacggc ggggtctggg agacggtcga cgccggccgc acctggcagc cgatcttcga
      181 cggccagccc atcggcgcca tcggcgcgat cgcgctcgcg ccctcggacc cgaaggtgat
      241 ctacgtcggc agcggagaag ccgacatgcg ctccgacatc gcccagggcg acggcatgta
      301 caagtctgcc gacgccggga agacctggac gaagatcggc ctccccgaca cgcagcagat
      361 cggccggatc ctcgtggacc cgagcgaccc gggccgggtg ctcgtggccg ccctcggcca
      421 tccctacggg cccaacgccg agcggggcgt gttccgctcc cccgacggag gcgctgcctg
      481 gcggaaggtc ctcttcaagg acgagaacac gggggccatc gacctcgcct tcgagcccgg
      541 cgactcccgg gtcgtgtacg ccgcgctgtg gcagacgcgc cgcccgccgt ggagcgtgta
      601 cccgccgtcg aacggccccg gcagcggcct ctacaagtcg accgacggcg gcgatacctg
      661 gaggccgctc gtcggccacg gcttccccgc gaggcccggc cgcatcgggc tcgccctctc
      721 tccgagccgt ccggagcggg tctacgccat ggttgacgcg cccgacggcg ggctctaccg
      781 ctccgacgac cgcggggcga gctggacccg cgcgagcggc gaccggcgga tctggggccg
      841 cgggtggtac ttcggcggga tcacggtcga gccgcgcgac ccggacgtgg tctacgcctg
      901 caacacctcg ctctatcgct cgcgcgacgg cgggaggacc ttcgtcccca tccagggagc
      961 gcccgggggc gacgactacc acgagctgtg gatcgacgcc gatcatccgg agcgccggat
     1021 gctcggggcc gaccagggcg cggtcgtgtc gctcaacggc ggccggacct ggagctcctg
     1081 gtacaaccag ccgacgggcc agatgtacca cgtcagcacc gacgaccggt tcccctactg
     1141 ggtgtacggc tcccagcagg actcgggggc agcagcggtt cccagccgca ccaccaccca
     1201 cgacggcatc accatgatgc agttccgcga gatgacggcc ggctccgaga gcgacgtgat
     1261 cgcccccgac ccgaaggacc cggacgtgat ctacgggggg cgggtggatc ggctcgacct
     1321 gcggacgcag cagacgcagt cgatcgaccc gacgctcctc caccgcgacg tcgatcgcgc
     1381 gacctggacc ctgcccctgg ccttctcgcg ccgcgacccg cgcgtcctct atttctcgcg
     1441 cgagcgactg ttccgcacga tggacggcgg ccgacactgg acggtgatca gccccgacct
     1501 cacgcgcgag gatcccgggg tgccggccac cctcgatccc gcggccgccg ccgacgcgcc
     1561 gcggcccgga aggcgtcacg gcgtcatcta cgcgatcgcg ccctcgcgcc tggccgacca
     1621 cgacctctgg atcggcaccg acgacggcct cgtctggcgc acccgcgacg agggcgcgca
     1681 ctgggagaac gtcacccccg ccgggctcgg cccctggtcg aaggtgggga tcatcgaccc
     1741 ctcgcacttc gacggggaga gcgcctacgc cgccgtcgac cgccaccggc tcgacgactc
     1801 caggccctac gtctaccgga cgcacgacgg gggccggacc tggaccctgg ccgcgaccgg
     1861 catccccgcg gggagcttcg tcaacgcggt gcgggaagat ccggtgcgca gggggctgct
     1921 ctacgccggc accgagaagg gcgtctgcgt gtcgttcgac gacggggacc actggcagcc
     1981 gctgcagctc aacctgccgg tcacctcggt gcgcgacatc gacgtccacg gcgacgacct
     2041 cgtgatcgcc acccacggcc gcgccttctg ggtcctcgat gacgtgaccc cgctgcgcca
     2101 ggccgggccc gacgtcgcgt ccgcccccgc gtggctcttc gctcccgcga ccgcgatccg
     2161 gctgcgcccc gccgggttca ccggcacccc gctgcccagg gacgagccca ccgccgccaa
     2221 ccctccgccg ggcgccgtga tcgactacgt gctctcgaag gcggggacgg cgccggtcgt
     2281 gctgcggatc ctggacgccc ggggcgcgct cgtgcgccgc tgctcgagcg acgagggccc
     2341 gcccgccccg gacctggcga agatccgggt ggccccggag tggatgacgc ccccggtggc
     2401 cctcaccacg aagcccggca tgcatcgctt cgtctggccc atccgctatc cggccccgcc
     2461 ggcgctctcc gagcacaacc tctacgccga cggcgtgtgg gcgccccccg ggcgctacac
     2521 ggtcgcgctg accgtggacc gccagcgcct cacgcagccg ctcacgatcg cgcccgaccc
     2581 gcgcgtcgcg ctcccgccgg aagcctacgc gcgtcagctc gccctcgcgc gccgcgtgga
     2641 ggcgctgcgc gagagcatcg ccgccgccct ggcggaggcg gagaagctgc atgtggatct
     2701 cgccgcgaag ggcgcgtccg acctcgacgc gcgcgtgcgg gccctcaccg gtcccgactt
     2761 cggcgaggct gcgacggcgg ggcctccggc cggactcagc agcctgcgcg cgctcgcgag
     2821 ctccctcgcc accctcgcca atgccgtgga cggcgcggac gcggagccca cccccgacgc
     2881 cgaggccgcc atccccaagg tcgagcaggc cgtgcaggcg acgctcgccg cgtgggcaac
     2941 gctcaagcag cagtggagcc cgtagaggag cggcatactc gggctcggag gcaatgaagc
     3001 cggactggac ggccgcaccc acccaccttc ccgctgtgcc cgcggcgtcg cggagcggag
     3061 ccacgctcgc ggccgggggc ccgggcgtcg gagttggagt gggcacggcg tagtcaccgc
     3121 ccccgccgca ggcggcgagc gccacgagca gcgctatcga ctcgcccctc aggccagcgc
     3181 ccccacggcc gcgaccgccg agtccacctc ctccggcgtg ttcaggatgc tccccgcgag
     3241 ccgggcgaag gactggagat aggggctcgt ggaggcgatg atcctccgct ctcgcaagcg
     3301 cctgacgacc tcttcgggcg tgacccccgc cacctcgaag gcgaccaggc cggcggagag
     3361 gccgggagcg cggggagtgt gcagggtcac gtggcgcagc ccggccaggc cgtccttgag
     3421 gcgcgtgttc atctcgtgga tgcggccggc gatccgggct cgcccgatct cgcggtggaa
     3481 ggcgaaggcg gcgggcagcg cccacatatg ctcgtacgcg tggaagccgc ccggcgacat
     3541 ccaggctccc tgggtcgggc cgggctccac cccgtgctgc cacgccatgt agggcggcat
     3601 ctcgaaggcg gggatgctcg gctgcactct ttcccaggcc tcgcgccgtc cccagacgat
     3661 cgcggtgccg cgaggcccga agatccactt gtgggctccg gcggcgagga agtcgcaacc
     3721 caggtcggcc gccgactcgt cctcgacccc gaagccgtga acgccgtcga ccacgagcag
     3781 cacgcgatcg gcagggtcgc gcgagcggtt cagctccgcc accgaggccg cgatcgaccg
     3841 gatgggcagc ttcaccccgg tggacgagtg gacccacgtg acgccgatca cccgcgtccc
     3901 ggggcgcacg gctcggcgga gccgatcgac gatctcgtcc tcggacgccc ggctgccctc
     3961 gtcgtagagc gccacccggc gcatctcggc accgtctcgc tgggcagcga gacgaatcga
     4021 ctcgtggtgc acgtagtggt cgtgcgccgt ggtcaggatc tcctggcccg accgcagccg
     4081 cagcccggcg tagatgaggg ccagcccttc ggtcgtggag cgggtgagag ccacgtcctc
     4141 gggccggccc ccgagataca cggcggcggc tcgtcggacc tcgtcgggcc gggtgaagag
     4201 gccgtggtca acgacctcga agggattctc gtcgagggcc cgtcgcaggg tctcgatcgc
     4261 ctcccgcacc gggcgaggat gcgacaccag gtagaagctc gcgaagtgca ggaggtcgcg
     4321 ccggaggtcg aactggtcgc gcacccaggc ccagtcctgg ggtgaggcgg tgctcgcggg
     4381 gcgagcgagc gcggtcgcgg ccaccgacat cccggtagcg acgaggaacc ggcgacggtc
     4441 caagggcatg ctgcctccgt tccgagatga cgaccattct gggcggacgg cgaccgcgcg
     4501 tcaaagccga aacgcgaccg gccggcgacg gggagcgccg ttgacgggcc gcctaattgt
     4561 tgctagattg gttacacatg ttcgccaact cgcacttcgc ccttggcacc cacgtgctgg
     4621 tgtcgctcgc gctccaggag ggctcacccg tcacctccgc cgagcttgcc cgcagcatca
     4681 acaccaaccc tgcctttctc aggatgctgc tggggaggct gcgggaggcc gggttgatcg
     4741 aggtggctct gggcaagggg ggcggcgcca ccctggcccg gcccgcgtcg aagctgaccc
     4801 tggccgacgt gtatcacgcg gtcgagcgcc agccgccggc ccgcctgcac cgctgcccgc
     4861 ccagtcgaag ctgcatcgtc ggtcgcaaca tcctccccgt gctccaggcc gtggttcatg
     4921 acgtggagtc ggcggccctg ggccggctca tggggatcac ggtcgcaaag ctggccgatc
     4981 aggtacggcg caggggctga cctggggagc cggagggcct cttcacgact ttttacggac
     5041 ctatatgtaa ctgtatcacg tacacacaag catgacgcat cccaatgacc gaggagggga
     5101 catgatgaga cggctcgaaa acaagatcgc gctgatcacg gggggcacga ccggcatcgg
     5161 gctggccacc gcgaaacgct tcgccgagga gggagcccag gtgatcgtga ccggtcggaa
     5221 cccggccacc ctcgagctcg cccggcggga gctcaacggt cgggtccagg tcctggaatc
     5281 cgacgccgcc gacgagcgcc aggtggcgga gctcttcgaa tcggtcggtc gcgagcacgg
     5341 gcggctggac gtgctgttcc tcaacgccgg gatcgcgaag ttcgctcccc tcgccgaggc
     5401 ccccctctcg gacttcgacg ccatgtggca cctgaacgtg cgcggcccct ggctggccct
     5461 caagcacgcc ctcccgatcc tcaccgaggg ggccgccgtc atcgtcaaca cgtcggtggt
     5521 caaccagaag ggaatggcgg gcacgacggc ctatgccgcg accaaggcgg ccctgcgcgt
     5581 gatcgtgcgc gcggcggctg ccgagctggc cgggcgcaag gtgcgagtga acgccatcag
     5641 cccggggccg atcgagactc cgatcttcga gaagacgggc ctttcggccc aggagcggga
     5701 ggcgtttcgg aaggacgtgg ccgcccgcgt cccgctcgga agattcgggg cggcccacga
     5761 ggtcgccagc gcagccgcgt tcctggcctc cccggacgca tcgttcatca cgggcaccga
     5821 gctcgccgtc gacggcgggt tcgcgcaggt gtgatgcggc agcggagtcg ggccctgtcc
     5881 gggcagggga tcggccgccg cgccgccgcg gccgatccag cgaagcaggt ggtacccgag
     5941 ggctccgccg accattcgca cggcggccgc gcctcggcga gtatcagaag aagaacttca
     6001 ccaccagctc gacgttgcgc ggcaggctga ccgtgttcgt gatcttgccg aacgtgttca
     6061 cgtccgcgat gttccggccc gggaaggtga agctcggggt gttggtcagg ttgaaggcct
     6121 cggcccgaac ctccaggtag cgctcgccgc ccatgtcgaa gcgcttggag agcacggcgt
     6181 cgatgttgcg gtagccgggc gctctggcgg tcccgacgcc gcaggtgccc cacgtcccga
     6241 ggggcgcccg cgagaaggcg ttgatgtcca gccagtggtc gatggtctgg tccgcgggct
     6301 tcgggtcgcc gatgcagttc ggccgctcga cgccgcgcac cgcctggagc gaagacccgc
     6361 ggctgtcctg gagggtcatc ggaacgccgc tgcgagcctg gaagatgccg ctcagcttcc
//
LOCUS       DASPWO010000259         5870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_549735, whole genome shotgun sequence.
ACCESSION   DASPWO010000259 DASPWO010000000
VERSION     DASPWO010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549735"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VEO00_05810"
     CDS             <1..223
                     /locus_tag="VEO00_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU57549.1"
                     /translation="GDQSTTRAAGGTGLGLYICRRLATALGGRLWLERTSATGSVFCL
                     WVPRTPPSGVFDERPAPAEPLRGEGAPGA"
     gene            complement(231..449)
                     /locus_tag="VEO00_05815"
     CDS             complement(231..449)
                     /locus_tag="VEO00_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57550.1"
                     /translation="MASRNQVAPSDVGKRVSFQYELPNGYVGEVVGVLRTFDDAADAY
                     VLIDRDGRLVRVPRAGVRFGRVVGPAPS"
     gene            complement(510..1787)
                     /locus_tag="VEO00_05820"
     CDS             complement(510..1787)
                     /locus_tag="VEO00_05820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010142767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HYU57551.1"
                     /translation="MATRDFRLPDLGEGLEEAEVTKWLVAEGDTVALNQPIAEVETAK
                     AVVEIPSPFAGTVVTLHAAEGAVVAVGAPLVTFEVGKEDAGEAAPPAAGDEGGDGAGP
                     KRQAVLVGYGVGVDEEPAAPAAPVGPKATERGRGRVRTTPPVRKLARELGVDLTAVRG
                     TGPEGRITREDVEAAAEGTTDAQPAGRRAVTPREDERVSVHGVRRTMARHMARAAAEI
                     PHVTTFVTVDCTWLLALRDELQAVAGEGTNVTPLAIVGRALAEVVREHPLLNASFDAE
                     REEIVLRGAFNLGIATDTERGLLVPVVKVADGLGIVGLASEIASVVAAARAGTATVEQ
                     LTGSTITITNVGTFGAEFGTPIINHPESAILALGVIEPRVLVVEGSVVARSACTLSLS
                     FDHRVLDGAQAGRAAAELRDLLQSPFRLGALPR"
     gene            complement(1787..2758)
                     /locus_tag="VEO00_05825"
     CDS             complement(1787..2758)
                     /locus_tag="VEO00_05825"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYU57552.1"
                     /translation="MTELTMASALNRALRDALAADERTLVLGEDVATLGGVFRITDGL
                     EAEFGPERVFDTPLAESGIAGVSLGLALAGWRPVAEMQFDGFSYPALDQVISHVAKHR
                     YRSRGTAPVPLVIRIPFAGGIGAAEHHSESPETYYAHTAGLKVVVPSSALDAYALLRA
                     SIEDPDPVVFLEPKSRYWSKETGEPGPGLPIGRARVVRQGADCTVVAYGAMVARSLAA
                     AERAAGRGLELEVLDLRSLVPLDVEALAESVRRTGRAVVVHEAPMTAGFGAEISARIM
                     EEAFDHLEAPVARVTGWDTPYPPATLEQHYLPSVQRILRAVDRIVEY"
     gene            complement(2751..3836)
                     /gene="pdhA"
                     /locus_tag="VEO00_05830"
     CDS             complement(2751..3836)
                     /gene="pdhA"
                     /locus_tag="VEO00_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HYU57553.1"
                     /translation="MSIPAGDGGDPFSILGENGTADERALSDLDLKEDELLALYRLMA
                     QTRRADLESTALQRQGELAVYPPLIGQEAAQVGSAFALGEEDFVFPSYRELGAALVRG
                     IDLVHYLYFYRATWHGGTYDPLERRFGMVSVPVGSQALHAVGWALGAKLDRKPVCSLV
                     YFGDGATSEGDVHEAWNFAGVFRAPVVFFCQNNQWAISVPVARQTAAPIWRKAEGYGF
                     PGILVDGNDVLAVYAVTREAAARAREDGTPTLIEARTYRLGPHSTADDAGRYQPSEEV
                     EAWRARDPLPRYERFLEAQGVLTDVAREEAARACDELAAHIRDGIVGAPPRPVAELFE
                     WVYEELPETLSRQRHEALELAGEEDDD"
     gene            complement(3833..4918)
                     /locus_tag="VEO00_05835"
     CDS             complement(3833..4918)
                     /locus_tag="VEO00_05835"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HYU57554.1"
                     /translation="MSIFEALGREYEEIVFFHDASSGLRAIVAIHSTVLGPALGGTRF
                     YPFETEEDALRDVLRLARGMTFKAAAAGLDLGGGKAVIVGDPKRIKSEELLRAYGRFI
                     DSLGGRYITAEDVGTSRDDMDLVRRETRFVTGVSPHLGGSGDPSPVTAIGVFEGLRAC
                     AQEVWGDPSLAGKRVAVQGVGKVGHHLVRLLVDAGAHCVVADVDVDAVARVVREFGVE
                     TAEPDKVHAVHCDVFAPCALGAVIRDDTLPELKCRVVAGSANNQLERPEHGRALHEAG
                     ILYAPDYVINAGGLINVSDELMGYSEERAMARVRGIYQTLRAVFHLASTKGIPTAEAA
                     DQFAAERMRKIGRLRLLHVPPRAGPSA"
     gene            5215..>5870
                     /locus_tag="VEO00_05840"
     CDS             5215..>5870
                     /locus_tag="VEO00_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57555.1"
                     /translation="MVNCAYLRVYRLLAAFPPDERARWERYIVGGGHVPPVRRVYRDQ
                     VTMVEGRVGLLAAAEGDYADIRFVDDTYFVCPWRTRLRVLASILSLRESAPPEVVEAF
                     VPEAEARRAARELARQRRRDPASMPSMLQSPWHVPIRWFVLFADEERRIVEREDGRHR
                     LFYWTGIGHARRRAERALRVLRRSELAPVAELVADLARWLSAFDPRSAVELDYDTVSD
                     "
BASE COUNT          748 a         2236 c         2095 g          791 t
ORIGIN      
        1 gggcgatcag tcgaccaccc gcgcggccgg cgggaccggc ctgggcctct acatctgccg
       61 ccggctggcg acggcgctcg gcggccggct gtggctggag cgcaccagcg cgacgggttc
      121 ggtcttctgc ctctgggtgc cgcgcacgcc tccgagcggc gtcttcgacg agcgccctgc
      181 cccggccgag cccctgcgcg gagagggcgc ccccggggcc tgaccggagc tcagctcgga
      241 gcgggtccga ccacccggcc gaagcgcacg ccggcccgcg ggacccgtac cagccggccg
      301 tcccggtcga tcaggacgta ggcgtcggcc gcgtcgtcga acgtgcggag gacgccgacg
      361 acctcgccga cgtagccgtt cggcagctcg tactggaacg agacccgctt cccgacgtcg
      421 cttggcgcga cctggttccg agaggccacg ggcctcacgc tacccgcggc ccggcggcgg
      481 ctcaagccgg cccgcaccgt gagtcgggct cagcggggga gcgcgcccag ccggaacggg
      541 ctctgcagaa ggtcgcgcag ttcggccgcc gcccgccccg cctgcgcgcc atcgagcacg
      601 cggtggtcga acgagagcga cagggtgcag gcggaccggg cgaccaccga tccctccacc
      661 acgagcaccc tcggctcgat gacgccgagg gccaggatcg ccgactcggg gtggttgatg
      721 atcggcgttc cgaactccgc gccgaacgtc ccgacgttcg tgatcgtgat cgtgctcccg
      781 gtgagctgtt ccaccgtggc cgtgccggcc cgggccgcgg cgaccacgga ggcgatctcg
      841 gaggcgaggc cgacgatgcc cagcccgtca gcgaccttca ccacagggac gagaaggccg
      901 cgctccgtat cggtggcgat cccgaggttg aacgcgccgc gcaggacgat ctcttcgcgc
      961 tcggcatcga acgatgcgtt cagcagcggg tgctcgcgaa cgacctcggc cagcgcccgg
     1021 ccgacgatcg cgagcggcgt gacgttcgtc ccctcgccgg ccaccgcctg caactcgtcg
     1081 cgcagcgcca gcagccaggt gcagtcgacg gtcacgaacg tcgtgacgtg cgggatctcg
     1141 gccgccgcgc gcgccatgtg gcgcgccatc gttcgccgga ccccgtggac cgacactcgc
     1201 tcgtcctcgc gcggagtgac cgcgcgccgg ccggcgggct gcgcgtccgt cgttccctct
     1261 gcggcggcct cgacatcctc gcgggtgatc cggccctcgg gcccggtccc gcggacggcg
     1321 gtgaggtcca cgcccagctc gcgggcgagc ttgcggacgg ggggcgtggt cctgacccgg
     1381 ccgcgcccgc gctccgtcgc cttcggcccg accggtgcgg ccggtgcggc cggctcctcg
     1441 tccaccccca cgccgtagcc caccagcacg gcctggcgct tcggcccggc cccgtccccg
     1501 ccctcgtcgc cggcggccgg cggagcggcc tcccccgcgt cctccttccc gacctcgaac
     1561 gtgacgagcg gggcgccgac cgccaccacc gcgccctcgg ccgcgtggag cgtgaccacc
     1621 gtgccggcga acggcgaggg gatctcgacc acggccttgg ccgtctccac ctcggcgatc
     1681 ggctggttca gggcgaccgt gtcgccctcg gccaccagcc acttcgtgac ctccgcctct
     1741 tccaggccct cccccaggtc gggcagcctg aagtccctcg tcgccatcag tactccacga
     1801 tgcggtcgac ggcgcgcagg atgcgctgca cgctcggcag gtagtgctgc tcgagggtgg
     1861 ccggcgggta cggcgtgtcc cacccggtga cccgcgccac gggggcctcc aggtggtcga
     1921 acgcctcctc catgatccgc gccgagatct cggcgccgaa cccggcggtc atcggcgcct
     1981 cgtgcaccac gaccgcgcgg ccggtcctgc ggacggactc ggcgagcgcc tcgacgtcga
     2041 gcgggaccag gctccgcagg tcgagcacct cgagctccag gccgcgcccc gccgcgcgct
     2101 cggccgcggc cagcgagcgg gccaccatgg ccccgtaggc gaccaccgtg cagtcggccc
     2161 cctggcggac cacccgcgcc cgcccgatcg gcagccccgg tcccggctcg ccggtctcct
     2221 tcgaccagta gcggctcttc ggctccagga acaccaccgg gtcggggtcc tcgatcgagg
     2281 cgcgcagcag cgcgtacgca tcgagcgccg aggacgggac cacgaccttc agcccggccg
     2341 tgtgcgcgta gtaggtttcc gggctctcgg agtggtgctc cgccgcgccg atcccgccgg
     2401 cgaaggggat acggatcacg agcgggaccg gggcggtgcc acgcgagcgg tagcgatgct
     2461 tggcgacgtg cgagatcacc tggtcgagtg ccgggtacga gaacccatcg aactgcatct
     2521 cggccaccgg gcgccacccg gcgagcgcca gcccgagcga gacgccggcg atccccgact
     2581 cggcgagtgg cgtgtcgaag acgcgctcgg gcccgaactc ggcctccagc ccgtcggtga
     2641 tccggaagac gccgccgagc gtggccacgt cctcgccgag gaccagcgtg cgctcgtcgg
     2701 ccgccagcgc gtcgcgcagc gcgcggttca gcgcggaggc catcgtcagc tcagtcatcg
     2761 tcctcctcgc cggccagctc gagcgcctca tggcgctgcc gggaaagcgt ctcgggcagc
     2821 tcctcgtaga cccattcgaa cagctccgcg accgggcgcg gcggcgcgcc gacgatcccg
     2881 tcgcggatgt gcgccgcgag ctcgtcgcag gcgcgggcgg cctcctcgcg cgcgacgtcg
     2941 gtcagcacgc cctgcgcctc gaggaaacgc tcgtagcggg ggagcgggtc gcgcgcccgc
     3001 cacgcctcga cttcctccga gggctggtag cggccggcgt cgtcggcggt cgagtgcggc
     3061 ccgagccggt aggtgcgggc ctcgatcaat gtgggcgtgc cgtcctctcg cgcccgggcc
     3121 gccgcctcgc gcgtcacggc gtacaccgcg aggacgtcgt tcccatccac gaggatgccg
     3181 gggaagccgt acccttcggc cttgcgccag atcggcgcgg ccgtctggcg ggccacgggg
     3241 accgagatgg cccactggtt gttctggcag aagaagacga ccggcgcgcg gaacacgccg
     3301 gcgaagttcc acgcctcgtg cacgtcgccc tccgacgtgg ccccgtcgcc gaagtagacg
     3361 agggagcaga ccggcttgcg gtccagcttc gcgccgagcg cccagcccac cgcgtggagc
     3421 gcctggctgc cgaccggcac cgacaccatc ccgaaccgcc gctcgagcgg gtcgtaggtc
     3481 ccgccgtgcc aggtggcccg gtagaagtac aggtagtgga ccagatcgat gccccgcacg
     3541 agcgccgcgc ccagctcgcg gtaggacggg aacacgaagt cctcctcccc gagggcgaag
     3601 gcgctgccga cctgggccgc ctcctggccg atcagcggcg ggtagacggc gagctccccc
     3661 tggcgctgca gcgcggtgga ctcgaggtcg gccctccgcg tctgggccat caggcgatac
     3721 agcgccagga gttcgtcctc cttcagatcc aggtcggaca gggcgcgctc gtcggccgtg
     3781 ccgttctcgc ccaggatgct gaaggggtcg ccgccgtccc cggccgggat gctcacgcgc
     3841 tcggcccggc tctcggcggg acgtggagga ggcgcagccg cccgatcttc ctcatccgct
     3901 cggccgcgaa ctggtcggcc gcctccgcgg tggggatgcc cttggtgctg gcgaggtgga
     3961 acacggcgcg cagcgtctgg tagatgccgc gcacccgggc catggcccgc tcctccgagt
     4021 accccatcag ctcgtcggag acgttgatca ggccaccggc gttgatcacg tagtcagggg
     4081 cgtagaggat gcccgcctcg tggagcgcgc gcccgtgctc ggggcgctcg agctggttgt
     4141 tcgccgagcc ggccaccacc cggcacttca gctcgggcag cgtgtcgtcg cggatcaccg
     4201 cgccgagcgc gcagggagcg aacacgtcgc agtggaccgc gtggaccttg tcgggctcgg
     4261 ccgtctcgac gccgaactcg cgcaccaccc gcgccacggc gtcgacgtcg acgtcggcca
     4321 ccacgcagtg cgcgccggcg tcgacgagca gccggaccag gtgatggccg accttgccca
     4381 cgccctgcac ggcgacgcgc ttgccggcca gcgagggatc gccccacacc tcctgggcgc
     4441 aggcccgcag cccttcgaac acgccgatgg cggtgacggg ggaggggtcg cccgagccgc
     4501 cgaggtgcgg cgagaccccg gtgacgaacc gggtctcccg ccgcaccagg tccatatcgt
     4561 cgcgcgaggt gccgacgtcc tcggccgtga tgtagcggcc gccgagcgag tcgatgaacc
     4621 ggccataggc ccgcagcagc tcctcagact tgatccgctt cgggtcgccg acgatcaccg
     4681 ccttgccccc gccgaggtcg aggcctgcgg cggcggcctt gaaggtcatg ccgcgcgcca
     4741 ggcgcaggac atcccgcagc gcgtcctcct cggtctcgaa cgggtagaag cgggtgccgc
     4801 cgagcgccgg cccgagcacg gtggagtgga ttgccacgat cgctcggagg ccgctcgacg
     4861 cgtcgtggaa gaagacgatc tcctcgtact cccgcccgag cgcctcgaag atgctcactt
     4921 ggctccgtgg ggaggagacc ctccccaccc cccacccgtc gggtccttcc gtgggtgagt
     4981 cacttggctc cgtggggagg agaccctccc cactccccac ccgtcgggtc cttccgtggg
     5041 ggtctcatcg cagctcccgg gaccgacccc ggtgtcggcg ctcggtcatg tgcgctcccg
     5101 gctcgagggg cggctgaccg gccgagtgta acgcacgccc ggaggcccac ccgaggagcc
     5161 cccgggcatc ggccgggcta cggtggggcc tgtgcccgcg cgtagaatcc ggccgtggtc
     5221 aactgcgcct acctgcgggt ctaccgtctg ctggcggcgt ttccgcctga cgagcgggcc
     5281 cgctgggagc gctacatcgt cgggggcggc cacgtcccgc cggtgcgccg cgtctaccgc
     5341 gaccaggtga cgatggtcga ggggcgcgtc gggctgctgg cggccgcgga gggcgactac
     5401 gcggacatcc gcttcgtcga cgacacctat ttcgtctgcc cctggcggac gcggctgcgc
     5461 gtgttggcga gcatcctctc cctccgcgag tcggcgccgc ccgaggtggt cgaggccttc
     5521 gtccccgagg ccgaggcacg ccgggccgcc cgggagctgg cgcggcagcg gcgccgcgat
     5581 ccggcctcga tgccctcgat gctccagagc ccctggcacg tcccgatccg ctggttcgtc
     5641 ctgttcgccg acgaggagcg ccggatcgtc gagagggagg acggtcgcca ccggctcttc
     5701 tactggacgg gtatcgggca cgctcgccgg cgcgcggagc gggccctgcg cgtgctgcgc
     5761 cgctcggagc tcgccccggt cgccgagctg gttgccgacc tggcccgctg gctgtccgcc
     5821 ttcgacccgc gctcggccgt cgagctcgac tacgacacgg tctcggacat
//
LOCUS       DASPWO010000260         4012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_887527, whole genome shotgun sequence.
ACCESSION   DASPWO010000260 DASPWO010000000
VERSION     DASPWO010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887527"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VEO00_05845"
     CDS             <1..403
                     /locus_tag="VEO00_05845"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667866.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HYU57556.1"
                     /translation="FNELVASGELSAPIVIGRDHLDAGSVASPYRETEGMRDGSDAIA
                     DWPILNALLNTAAGAHWVSVHHGGGVGIGYSIHAGMVVVADGTEDAARRLERVLVTDP
                     GLGVVRHADAGYERALEVAAERGIALPWREP"
     gene            complement(796..1239)
                     /locus_tag="VEO00_05850"
                     /pseudo
     CDS             complement(796..1239)
                     /locus_tag="VEO00_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018959703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
     gene            1454..1696
                     /locus_tag="VEO00_05855"
     CDS             1454..1696
                     /locus_tag="VEO00_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HYU57557.1"
                     /translation="MRRALDGTSYMIRFTPRTFEPGQEKAFRANDRAWAFWQGQPAGY
                     RRTATFWVTSARREETRARRLATLIETPNRATASRR"
     gene            1808..3154
                     /locus_tag="VEO00_05860"
     CDS             1808..3154
                     /locus_tag="VEO00_05860"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HYU57558.1"
                     /translation="MRIAIVGAGHVGLVSGACFAAVGHDVVALDVDRERVAMLEAGEA
                     PFYEPGLAELLAASRASGRLRFTADPAQGLAGAELAFVCVATPNDARGHVDLSAVVAA
                     ARTVARHGEPEAVLVNRSTAPVGTARFLRSLVEEEREGEVLVAVNPEFLAEGSGVRDF
                     LFPDRIVIGAWEPRAAERLHAAYAPIVAGDVPDGLPDGIAPTQRRAGPVPLVETNPPT
                     AELIKYATNAFLAMKISFINEMAGIAEEVGGDVTEVSRAVGLDRRIGPRFLRAGIGWG
                     GSCFPKDIEALQGMAATHGLEARLLKAANDVNADQHRWVIRKLQRHLKTLVGRRVAML
                     GLAFKPDTDDVRNAPALEIAHELARSSVRVRAYDPVVKAIPEELDGSIELVADPISAA
                     RGAEAVVLVTEWPEFRGLDLEALGAVMRERLLLDGRNYLDPEAVRAAGFTYVGVGR"
     gene            complement(3247..3513)
                     /locus_tag="VEO00_05865"
     CDS             complement(3247..3513)
                     /locus_tag="VEO00_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57559.1"
                     /translation="MDAVILAALAALAVLAGLSVGTLFSLGARLDALGSRIDALSPRF
                     DALGGRIDSLSGRMDGFSSRMDEFSARMDGFSDRLESHVSRNAG"
     gene            complement(3551..>4012)
                     /locus_tag="VEO00_05870"
     CDS             complement(3551..>4012)
                     /locus_tag="VEO00_05870"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYU57560.1"
                     /translation="GDGIAPVREMLERGIPVCLGTDGGCTNSRQSVFEEMRMAALLQK
                     GRLADAAALKAEDAMRMGTVRGGEALGLPIGRIAPDHAADLVVLDLASLSLLPEATAP
                     KQVVYAMQPDAVRRVIVAGEAIVEDGHILKADEAEIRAKVNEITKDWTPPD"
BASE COUNT          556 a         1429 c         1499 g          528 t
ORIGIN      
        1 gttcaacgag ctggtcgcga gcggcgagct ttcggcgccg atcgtgatcg ggcgcgacca
       61 cctcgacgcc gggtcggtgg ccagccccta ccgcgagacc gaggggatgc gcgacggctc
      121 agacgccatc gccgactggc cgatcctgaa cgcgctgctc aacacggcgg ccggcgcgca
      181 ctgggtgagc gtccaccacg gcggcggggt ggggatcggc tactcgatcc acgccgggat
      241 ggtcgtcgtc gccgacggca cggaggacgc cgcccgccgc ctggagcgcg tcctggtgac
      301 cgaccccggc ctgggggtcg tccgccatgc cgacgccggc tacgagcgcg cgctcgaggt
      361 cgccgcggag cggggcatcg cgctcccgtg gcgggagccc tgagcgggag ggccgacggc
      421 acgcgggcgc gctcgggacg ggagccctcc gtcagccagc agcagcacca gggagagcgc
      481 gatcgccccg gacgaacgga cggtgcagcc ctccgtcagc gcagggttac ggggagggaa
      541 agcacggatg ggacggatcg gatcgcacga tccgttccgg atccgatggc cgcatcagtc
      601 ctggggcggc agcacgccga gggaggttcc gcagcgcccg cagaacacga acaggacccg
      661 gcgggcgggc tgatccccac ggcccggcac ctcgagcacc tccgctcgcc cctggtccac
      721 cagctcggcg cggcactgtg ggcaccgcgc ggtgcggatc gccgggggct cctcgacgga
      781 gggtccgtcc tcggcgccgc cggcatggcc ggacaggagc tcggtgaccc gctcgcggac
      841 gcggtccagg tcggcgccga acccgtgcag gacctgggcg gcaacgccat cgccctcccg
      901 gatcaggccg agcaggatgt gctcggtgcc gatgtagttg tggccgagct gcagcgcctc
      961 gcgcagcgac agctcgagga ccttcttggc ccgcggcgtg aaggggatgt gcccggtcgg
     1021 aggcgagctt ccgcggccga tcttctcctc gacctgccgc cggacggcct cgagcgagac
     1081 gtcgagcgcc tgtaacgcgc gggcggccac gccctcgccc tcgtggatca ggccgagcag
     1141 gaggtgctcg gtgccgatgt agttgtggtc gaggaggcgc gcctccgcct gggcgaggac
     1201 gaccacgcgc cgggcccggt cggtgaaccg ctcgaacatc cacgccaggg tagcctgccc
     1261 ggacgcccgg cagcatcggg cgagtggggc cccgctcctt cgcgtcaccc gccgcgctgc
     1321 gtcgctgctt cgggcgcaac gacggccgca gcgccgagct gttggtgggg ttccacaggg
     1381 tcggtccggc aggcccggca tcacctaccg gcaggcgctc gacgaggcgc tgtgcttcgg
     1441 gtggatcgac ggcgtgcgca gggctctcga cgggacgagc tacatgatca ggttcacgcc
     1501 gcggaccttc gagccggggc aggagaaggc gttccgcgcc aacgatcgcg cctgggcgtt
     1561 ctggcagggg cagccggcgg ggtaccgccg gaccgccacg ttctgggtca cgagcgccag
     1621 gcgcgaggag acccgagccc gccgcctggc cacgctgatc gagactccga acagggccac
     1681 cgcgtcgcgc cgctgacgcg cccgtcggag cggacggggc ggagcaccgg atcggcgcct
     1741 tctggttcgt ggcaggacgg gcagtagact ggcggcccgc ccgctcgagg tggaggaccc
     1801 acgacccatg cggatcgcga tcgtcggcgc cgggcacgtc ggactggtca gtggcgcgtg
     1861 cttcgcggcc gtcggccacg acgtggtcgc actcgacgtg gaccgtgagc gggtggccat
     1921 gctcgaggcc ggcgaggcgc cgttctacga gccgggcctc gccgagctgc tcgcggcgtc
     1981 ccgcgccagc gggcgcttgc ggttcacggc cgatccggcg cagggcctgg ccggcgcgga
     2041 gctcgccttc gtctgcgtgg ccacgccgaa cgacgcgcgg gggcatgtcg atctctccgc
     2101 ggtggtggcg gcggcgcgga ccgtggcccg ccacggcgag cccgaagccg tcctggtcaa
     2161 tcgcagcacg gcgccggtcg ggacggcccg gttcctgcgc tccctggtcg aggaggagcg
     2221 cgagggggag gttctggtcg cggtgaaccc ggagttcctg gccgaggggt cgggcgtgcg
     2281 cgacttcctg ttcccggacc ggatcgtgat cggcgcctgg gagccccgcg ccgccgagcg
     2341 cctccacgcg gcgtacgccc cgatcgtggc cggggatgtg ccggacggac tccccgacgg
     2401 catcgctccc acgcagcggc gagccggccc ggtgccgctc gtcgagacga accccccgac
     2461 ggcggagctg atcaagtacg ccaccaacgc gttcctggcg atgaagatct cgttcatcaa
     2521 cgagatggcg gggatcgccg aggaggtcgg cggcgacgtc accgaggtct cgcgcgcggt
     2581 cgggctggac cggcgcatcg ggccgcgctt cctgcgcgcc gggatcggct ggggcgggtc
     2641 ctgcttcccg aaggacatcg aggcgctgca ggggatggcc gccacccacg ggctggaggc
     2701 gcgcctgctg aaggcggcga acgacgtgaa cgcggaccag caccgctggg tgatccgcaa
     2761 gctgcagcgc cacctgaaga ccctcgtcgg gcggcgcgtc gcgatgctcg ggctggcgtt
     2821 caagcccgac accgacgacg tgcgcaacgc gccggcactg gagatcgccc atgagctggc
     2881 gcgctccagc gtgcgcgtgc gcgcgtacga cccggtggtg aaggcgatcc cggaggagct
     2941 cgatggttcg atcgagctgg tcgccgaccc gatctcggcc gcgcgcggcg ccgaggcggt
     3001 cgtgctcgtc accgagtggc ccgagttccg aggcctcgac ctcgaggccc tcggcgcggt
     3061 gatgcgtgag cgcctgctcc tcgacggccg caactacctc gaccccgagg ccgtccgcgc
     3121 cgccggcttc acctacgtcg gcgtcggccg ctagattcag cggaatgtca agcgggcccg
     3181 gctggcacaa ctggctgtcg ccggcttcac cgcggtcggc gtcggaggca gtcgcctcct
     3241 tgccgatcac ccggcgttgc ggctgacgtg ggattccaag cggtcggaga acccgtccat
     3301 acgggccgag aactcgtcca tgcggctcga gaagccgtcc atccgacccg agagcgagtc
     3361 gatgcgccca cccagcgcgt cgaagcgagg gctcagggca tcgatgcgag agcccagggc
     3421 gtcgaggcga gcgcccaggg agaagagcgt gccaacgctc aatccggcaa gcaccgcgag
     3481 cgccgcgagc gccgcgagga tcaccgcgtc catctccggg accatagcat ccgggtacga
     3541 cacggattcc tcaatcgggc ggggtccagt ccttcgtgat ctcgttcacc ttggcacgga
     3601 tctcggcttc gtcggccttc aagatgtggc cgtcctcgac gatcgcctcg ccggcgacga
     3661 tcacccgacg gacagcgtcc ggctgcatcg cgtagacgac ctgcttcggg gccgtcgcct
     3721 ccggcaggag cgagagcgac gcgaggtcga gcacgacgag gtcggcggcg tggtcggggg
     3781 cgatccggcc gatcgggagc ccgagcgcct ccccgccgcg caccgtcccc atccgcatgg
     3841 cgtcctcggc cttcagcgcg gcggcgtcgg ccagccgtcc cttctgcagc agcgccgcca
     3901 tccgcatctc ctcgaacacc gactgccggc tgttggtgca gccgccgtcg gtgccgaggc
     3961 agaccgggat gccgcgctcg agcatctccc gcaccggcgc gatgccatcg cc
//
LOCUS       DASPWO010000261         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1224040, whole genome shotgun sequence.
ACCESSION   DASPWO010000261 DASPWO010000000
VERSION     DASPWO010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224040"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VEO00_05875"
     CDS             <1..1036
                     /locus_tag="VEO00_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYU57561.1"
                     /translation="EAVLHVIERIVAPLGLRVDESSPWVDARLPDGSRVHAVIPPLSL
                     CGPVLNIRKFSLIPFTSDDLVRDGTMGPRMLDFLARCVRGKANLVISGGSSSGKTTLL
                     NVLASFIPDGERLITIEDAAELRLGKPHVVRLEARPANVEGKGEVTVRDLVRNALRMR
                     PDRIIVGEVRGGEALDMLQAMNTGHEGSLSTAHANSPHHLLWRLETMALMSDVDLPAA
                     HVREQVAAAIDVVVHMARLRDGRRVVLDISSVEGTAGGEPVVREVFAFQRGGGAPAGR
                     FVSTGRVPGVMGVLAERGEPMPPSLFDPGPDVAPPVPDRPRPGAPLPEPVPEAPPQDL
                     VRIPITVGDP"
     gene            1033..1911
                     /locus_tag="VEO00_05880"
     CDS             1033..1911
                     /locus_tag="VEO00_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYU57562.1"
                     /translation="MSQEVRFAILVALATVMVGSALGGIDRQRTLGRAGARRERSPAP
                     KRRGAPSIRAAAAGVVRWLGGGRPAAMFACGLSGAGIGIVVAAGPGGAICGAAGLAVP
                     GWIRSRRSGRRAALVEQQLADAMDGIAAALRAGMSPVRALSYAAEEGEPPVAAGLAAV
                     VEREELGMPLDQSLETWARSEQGDDVRLAVSVLQLQHRVGGNAPAVLEEAVRTLRQRA
                     SAARDLRSMTAQARLSGAILGLLPVGFFLFMSVVSRHEVTLALRTPLGATSVAGGLLL
                     DGAAFLWIRHLLREAQ"
     gene            1914..>2459
                     /locus_tag="VEO00_05885"
     CDS             1914..>2459
                     /locus_tag="VEO00_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57563.1"
                     /translation="MGGAEILGGAALAAAAASATALAVGWRLEPVVAERMFAGAPAGQ
                     SRTAGPGVAARLGARSWLLRVARPAMLHERFAAAGLAGDVHAYVGRKALVAAATLTVS
                     LVGPSPLPLLGPVLAAAGFVVPDMVLGRLARRRRAGMAAQLPQFLDLLAAGSTAGLSA
                     LLALRRATEAMRDPLAAELRGV"
BASE COUNT          271 a          857 c          969 g          362 t
ORIGIN      
        1 ggaggccgtg ctgcacgtga tcgagaggat cgtggccccg ctgggtcttc gggtcgacga
       61 atcgtcaccc tgggtggacg cgcgacttcc ggacggcagc cgggtccacg cggtcatccc
      121 gcccctttcg ctctgcggtc ccgtcctgaa catcaggaag ttctcgctga tcccgttcac
      181 ctcggacgac ctcgtcaggg acggcacgat ggggccgcgg atgctggact tcctggcccg
      241 gtgcgtccgc ggcaaggcca acctggtgat ctccgggggg tcgagctcgg gaaagaccac
      301 gctgctcaac gtccttgcgt cgttcatccc cgacggggag cgcctgatca cgatcgagga
      361 cgccgccgag ctccgactag ggaagcctca tgtcgtccgc ctcgaggccc gcccggcgaa
      421 cgtcgagggt aagggcgagg tcaccgttcg cgatctcgtc cgcaacgccc tgcgcatgcg
      481 ccccgaccgg atcatcgtcg gtgaggtccg gggcggggag gccctggaca tgctccaggc
      541 catgaacacg ggacacgagg gctcgttgtc cacggcgcac gcgaactcgc cgcaccacct
      601 gctgtggcgc ctcgagacca tggccctgat gtcggacgtc gatctgccgg cggcccatgt
      661 ccgggagcag gtcgccgcgg cgatcgacgt ggtggtgcac atggcccggc tccgcgatgg
      721 acgccgggtc gtgctcgaca tcagctccgt ggaggggacg gccggcggcg aacccgtcgt
      781 ccgcgaggtg ttcgcgttcc agcgcggcgg aggggctcca gcgggcaggt tcgtgtcgac
      841 cggccgggtg ccgggcgtca tgggcgtgct cgccgagagg ggggagccca tgccgccgtc
      901 gctgttcgat ccgggaccgg atgtcgcgcc gccggtcccg gatcgacccc gtcccggcgc
      961 gccacttccg gaaccggttc ccgaggcgcc gccgcaggac ctcgtgcgga tcccgatcac
     1021 ggtgggggac ccgtgagcca ggaggttcgg ttcgcgatcc tcgtcgcgtt ggcgaccgtg
     1081 atggtggggt cggccctcgg cgggatcgat cgccagcgaa ccctcggacg cgccggtgcg
     1141 cggcgggaac ggtccccggc gccgaaacgg cggggtgctc cctcgatccg ggcggccgcg
     1201 gccggggtgg tgcggtggct cggcggcgga cggcccgcag ccatgttcgc gtgcgggctc
     1261 tcgggcgcgg gtatcgggat cgtcgtggcg gccgggcccg gcggggccat ctgcggcgcg
     1321 gcgggtctcg ccgtgcctgg atggatccgg tcgcgccgct cggggcgccg ggcggcgctc
     1381 gtcgaacagc agctcgcgga cgcgatggat gggatcgccg ccgcgctgcg ggccggcatg
     1441 tcgccggtcc gggcgctctc gtacgcggcc gaagaggggg agcccccggt cgccgccggg
     1501 ctggcggcgg tcgtcgaacg cgaggagctg ggaatgccgc tcgaccaatc gttggagacg
     1561 tgggcccggt ccgagcaggg cgacgacgtc cgcctcgcgg tgagcgtcct gcagctgcag
     1621 caccgcgtgg ggggaaacgc ccccgcggtg ctcgaggagg cggtccgcac cctgcggcag
     1681 cgggcctcgg cggcccggga cctgcgatcc atgaccgccc aggccaggct ctccggcgcg
     1741 atcctggggc tgctcccggt cgggttcttc ctgttcatgt ccgtggtgtc ccgccacgag
     1801 gtcacgctcg cgctccgcac gccgctcggg gcgacgtccg tggccggagg gctgctgctg
     1861 gacggggcgg cgttcctgtg gatccggcat ctgctgcggg aggcgcagta gaggtgggtg
     1921 gcgccgagat cctgggaggg gccgcactcg ccgcggcggc agcctcggcg accgcactcg
     1981 cggtggggtg gcggctcgaa cccgtcgtgg ccgagcggat gttcgccggg gcgccggccg
     2041 gccagtctcg cacagcgggg cccggggtgg cggcgcggct gggggccagg tcgtggctcc
     2101 ttcgggtggc cagacccgcg atgctccacg agcggttcgc cgccgcgggg ctggccggtg
     2161 acgtccacgc gtacgtcggc cggaaggcgc tcgtggccgc cgcgacactg accgtttcgc
     2221 tggtggggcc gtctccgcta cccctgctcg gcccggtgct ggccgcggcc ggcttcgtgg
     2281 tcccggacat ggtgctgggc cggctggctc gccggcgccg cgccgggatg gccgcccagc
     2341 tgccccagtt cctcgacctg ctggccgcag gatcgacggc cgggctgtcg gcgctgctcg
     2401 cactccgccg cgcgacggag gccatgcggg atcccctggc ggcggagctc cggggcgtc
//
LOCUS       DASPWO010000262         4652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_806932, whole genome shotgun sequence.
ACCESSION   DASPWO010000262 DASPWO010000000
VERSION     DASPWO010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806932"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VEO00_05890"
     CDS             complement(<1..718)
                     /locus_tag="VEO00_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HYU57564.1"
                     /translation="MIDLKSVREDPAPARAGLARRGRAEDLDRLLAIDEEWRGLTAEV
                     EALRAEQNRVSKEVGRAEATERERLIASVRDLTDRLSVLEARLADVAARRDRLLLEVP
                     NPPHPSVPDGESDEDNEVVRVVGQVPERPWAADHVDLGTALGVLDLDRAVRLSGSRFA
                     YLTGPGVWLEFALVRYALDLVAAKGFVPVVPPVLVREEAMYGTGFLPADEAQIYVTRE
                     DGLYLVGTSEVALAGLHQEEI"
     gene            complement(715..1218)
                     /locus_tag="VEO00_05895"
     CDS             complement(715..1218)
                     /locus_tag="VEO00_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HYU57565.1"
                     /translation="MIWRRDDAAAPEPPAPPQPREVGVTDQGTGTADATIVGQAARLE
                     GTLVSAGALRVDGKVKGKINADGDVTLSPSAQVEADIRAENVTVAGRFKGNVTAAGRA
                     EIQRGGRVDGNITAKSLVVAEGAVFNGQSIMDQQATAPEGSDAKPAPAPARRAPETRA
                     AASPAGT"
     gene            complement(1238..1729)
                     /locus_tag="VEO00_05900"
     CDS             complement(1238..1729)
                     /locus_tag="VEO00_05900"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HYU57566.1"
                     /translation="MTLSNQTLTLLVLASLGLNVLLLFLLGVSRAGRRDGGIGGAPGD
                     GLVGAVDQHGRSLDRLTATVAELGEDQRRLAATVEGAVQHVAVVRFDAFGDMGGRLSF
                     AAALLDARGDGVVVSAINGRTDTRVYAKAVRGGTSVHNLSEEEEIAIREAFGAARQAV
                     KAK"
     gene            1790..2545
                     /gene="larB"
                     /locus_tag="VEO00_05905"
     CDS             1790..2545
                     /gene="larB"
                     /locus_tag="VEO00_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HYU57567.1"
                     /translation="MDERSLRFLLAAVRAGRLSPDQAVERLKDLPFVRAGESRPDLHR
                     ELRTGQAEAVYGPGKTPAEVRDILAALLKTPGGPVFVTKASADQAAAARAVAPDAEYH
                     PRSGLLVARVTDEAPAGTVLIVTAGTADLPVAEEATETARALGLKADLVADVGVAGVH
                     RLLAHRAALEAADCLIVVAGMEGALPSLVAGLTSRPVVAVPTSVGYGASFDGLAALLA
                     MLNACAAGVGVVNIDNGFGAAQLAHRIVRRRDV"
     gene            2455..3768
                     /gene="larC"
                     /locus_tag="VEO00_05910"
     CDS             2455..3768
                     /gene="larC"
                     /locus_tag="VEO00_05910"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HYU57568.1"
                     /translation="MRRRRRRGEHRQRVRRRAARPPDRPAAGRLAPRSEAAVRLAYFD
                     CIAGIAGDMTLGALVDAGADFDRLQEVLLGLPIEPFEIGRERVERQGVRAVKVHVHAP
                     DASVIRTYASIRNLLDAAELPEDARRIAHRIFRRLAEAESRVHGKEIELVTFHEIGAL
                     DSIVDIVGVAVAFSLLGVERVFSSPVPTGFGIHRTEHGVFPIPGPAVVELLRGVPMFS
                     RGVPVELVTPTGAAILAALAEGYGDMPTMRVEAVGYGAGTHKLDFPNVLRVLVGEEEV
                     APAPIPDAAGVRPTDLVLETNVDDLNPELHGYVLERLFSAGVQDAWLTPIVMKKGRPG
                     VTLSVLCSPEQEETVKEIVFGETGTLGVRVHAVAKHPLEREPMKVETPWGPVGVKIGR
                     RNGRVVSVGPEFEDCARVAREAGVPAKDVYAEALRLAREALGLQG"
     gene            complement(3801..4562)
                     /locus_tag="VEO00_05915"
     CDS             complement(3801..4562)
                     /locus_tag="VEO00_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYU57569.1"
                     /translation="MAKVEVEREGAVWTLTLNRPEVRNAVDAETAALLEQAIGAFAAD
                     DVARAMVVTGAGEAFCAGADLKAMDLLARPGLERSSPMGFGRLDPGKPTFAAVNGGCF
                     AGGMELAAWCDVRIADDRAEFGVLNRRWGIPLVDGGTQRLPRIMGLGNALYLIETGVR
                     IGARRALELGFVQEVVPAGTALRRAGELAAVAAAYPAASLRADRASALAALASPLAEG
                     LEREARDGLATIGDPEMTEELRRFAAGDLPEPPRG"
BASE COUNT          571 a         1780 c         1669 g          632 t
ORIGIN      
        1 ggatctcctc ctggtgcagc ccggccagcg cgacctcgct ggtgcccacg aggtacaggc
       61 cgtcctcgcg ggtgacgtag atctgcgcct cgtcggccgg caggaagccc gtcccgtaca
      121 tcgcctcctc gcggaccagc accggcggca ccaccgggac gaaccccttc gcggccacca
      181 gatcgagcgc gtagcggacc agggcgaact cgagccacac ccccgggccg gtcaggtagg
      241 cgaaccgcga gcccgacagc cgcacggcgc gatcgaggtc cagcacgccg agcgcggtgc
      301 cgaggtccac gtggtccgcc gcccacggcc gctccgggac ctgcccgacc acgcggacca
      361 cctcgttgtc ctcgtcgctc tccccgtcgg gcaccgaggg gtgcggcggg ttggggacct
      421 cgagcagcag ccggtcgcgc cgcgcggcca cgtccgccag gcgcgcctcc aggacggaca
      481 gccggtcggt gaggtcccgc acggaggcga tcagccgctc gcgctcggtc gcctccgcgc
      541 gaccgacctc cttcgacacg cgattctgct cggcgcgcag cgcctcgacc tcggccgtca
      601 gcccgcgcca ctcctcgtcg atggcgagga gccggtcgag atcctcggcg cggccgcgcc
      661 tggcaaggcc cgcccgcgcc ggggcgggat cctcgcgaac ggacttcagg tcgatcaagt
      721 accggccggg gaggccgccg cccgcgtctc gggcgccctg cgcgccggcg ccggggccgg
      781 cttggcgtcg ctgccctcgg gggccgtggc ctgctggtcc atgatgctct gcccgttgaa
      841 caccgcgccc tcggcgacga cgagcgactt cgcggtgatg ttgccgtcca cccgaccgcc
      901 gcgctggatc tcggcgcggc cagccgccgt gacgttgccc ttgaaccgcc cggcgaccgt
      961 cacgttctcg gcgcggatat ccgcctccac ctgcgccgag ggcgacaggg tgacgtcgcc
     1021 gtcggcgttg atcttgccct tcaccttgcc gtccacgcga agcgcaccgg ccgagacgag
     1081 cgtcccctcg agcctcgccg cctgaccgac gatcgttgcg tccgccgttc ccgttccctg
     1141 atccgtcacg ccgacctccc gtggttgcgg cggcgcgggc ggctcggggg ccgccgcgtc
     1201 gtctcgcctc cagatcatgg tgccctccga tccgggctca tttggccttc acagcctgcc
     1261 gcgcggcgcc gaaggcctcg cggatcgcga tctcctcctc ctccgacagg ttgtggacgc
     1321 tcgtcccccc gcgcacggcc ttggcgtaga cgcgcgtgtc cgtgcgcccg ttgatcgcgc
     1381 tgaccaccac gccgtcgccc cgggcgtcga ggagcgcggc ggcgaacgac agccggccac
     1441 ccatgtcccc gaaggcgtcg aaacggacca cggcgacgtg ctggacggcc ccctccacgg
     1501 tggccgcgag ccttcgctgg tcctccccca gctcggccac ggtggcggtc aggcggtcga
     1561 ggctgcgccc gtgctggtcg acggctccga ccagaccgtc ccccggcgcg ccgccgatgc
     1621 cgccgtcccg ccgccccgcc cgggagaccc cgagcaggaa caggagcagc acgttcagcc
     1681 ccaggctggc cagcacgagc agcgtgagcg tctggttcga cagcgtcatg agtgaaaagg
     1741 gcctctgacc tgcccttctg atgtggcgga gtatacactc cggcctgtcg tggacgaacg
     1801 ttccctccgg ttcctgctgg ccgcggtccg cgccgggcgc ctgtcacccg accaggccgt
     1861 cgagcggctg aaggacctcc cgttcgtccg ggccggcgag tcccgccccg acctgcaccg
     1921 cgagcttcgc accgggcagg cggaggccgt gtacgggccg ggcaagacgc ccgccgaggt
     1981 cagggacatc ctcgccgcgc tcctcaagac gccgggcggg ccggtgttcg tgaccaaggc
     2041 gtccgctgat caggcggccg cggcgcgcgc ggtggcgccc gacgccgagt accacccgcg
     2101 ttccggcctg ctggtcgccc gggtgaccga cgaggcaccc gcggggacgg tgctcatcgt
     2161 caccgcgggg accgccgacc tgccggtggc cgaggaggcg accgagaccg cccgcgcgct
     2221 cgggctgaag gccgacctgg ttgccgacgt cggcgtcgcc ggggtgcacc ggctcctggc
     2281 gcaccgcgcc gcactggagg ccgccgactg cctgatcgtg gtggccggca tggagggggc
     2341 gctcccgtcg ctcgtcgccg ggctgacgtc gcggccggtg gtggccgtgc cgacgagcgt
     2401 cggctacggc gcgtcgttcg acggcctggc cgcgctgctg gcgatgctga acgcttgcgc
     2461 cgccggcgtc ggcgtggtga acatcgacaa cgggttcggc gccgcgcagc tcgcccaccg
     2521 gatcgtccgg cggcgggacg tctagcgccg cgatcggagg ccgccgtccg cctcgcctac
     2581 ttcgactgca tcgccggtat cgccggcgac atgaccctcg gcgcgctcgt cgacgccggc
     2641 gccgacttcg accggctgca ggaggtgctg ctcggcctcc cgatcgagcc gttcgagatc
     2701 ggccgcgagc gggtggaacg ccagggcgta cgggcggtga aggtccacgt ccacgcgccg
     2761 gacgcgagcg tgatccgcac ctacgccagc atccgcaatc tcctcgacgc cgcggagctc
     2821 cccgaggacg cccggcgaat cgcgcaccgt atcttccgcc ggctggccga ggcggagtcg
     2881 cgggtccacg ggaaggagat cgagctggtc acgttccacg agatcggcgc gctcgactcg
     2941 atcgtggaca tcgtcggcgt cgcggtggcg ttctcgctgc tcggcgtgga gcgggtgttc
     3001 tcctcgccgg tcccgactgg cttcggcatc caccgcaccg agcatggcgt cttcccgatc
     3061 cccggcccgg cggtggtcga gctgctccgc ggggtgccga tgttctcgcg cggggtgccg
     3121 gtggagctgg tcacgccgac cggtgcggcc atcctggcgg cgctggccga gggctacggc
     3181 gacatgccga cgatgcgcgt cgaggccgtc ggctacggcg ccggcacgca caagctcgac
     3241 ttcccgaacg tgctgcgcgt cctggtcggc gaggaggagg tcgccccggc tccgatcccc
     3301 gacgccgccg gcgtccggcc gaccgacctg gtgctcgaga cgaacgtcga cgacctcaac
     3361 cccgagctgc acggctacgt gctcgagcgg ctgttctcgg cgggcgtgca ggacgcgtgg
     3421 ttgacgccca tcgtgatgaa gaagggccgg cctggagtga cgctctcggt gctctgctca
     3481 cccgagcagg aggagacggt caaggagatc gtcttcggcg agaccgggac gctcggggta
     3541 cgcgtccacg cggtggcgaa gcacccgctg gagcgggagc cgatgaaggt cgagacgccg
     3601 tggggcccgg tcggcgtgaa gatcgggcgg cgcaacggcc gggtcgtctc ggtggggccg
     3661 gagttcgagg actgcgcgcg ggtcgcccgt gaggccggcg tccccgcgaa ggacgtctac
     3721 gcggaggcgc tccgcctggc ccgcgaggca ctcggcctgc agggatagaa acccctgatg
     3781 ccgggcccgt tcggccgtgg tcagccgcgg ggtggttccg ggaggtcgcc tgccgcgaac
     3841 cgccgcagct cctcggtcat ctccgggtcc ccgatcgtgg ccagaccgtc gcgggcctcg
     3901 cgctccagcc cctcggccag cggcgaggcg agcgccgcga gcgccgaggc gcggtcggcg
     3961 cgcaggctcg cggcagggta ggcggcggcc acggccgcca gctcccctgc ccgccggagc
     4021 gcggtccccg ccgggacgac ctcctggacg aaccccagct cgagagcgcg ccgcgccccg
     4081 atccgcacgc cggtctcgat caggtacagc gcgttcccca ggcccatgat ccgtggcagg
     4141 cgctgggtgc cgccgtcgac gagcgggatc ccccagcggc ggttcaggac gccgaactcg
     4201 gcgcggtcgt ctgcgatgcg cacgtcgcac caggcggcga gctccatgcc cccggcgaag
     4261 cagccgccgt tcacggcggc gaacgtgggc ttgccggggt cgagccgccc gaaccccatc
     4321 gggctggagc gctccaggcc cggccgcgcg agcaggtcca tcgccttcag gtcggccccg
     4381 gcgcagaacg cctcgcccgc gccggtcacc accatcgccc gcgccacgtc gtcggccgcg
     4441 aaggcgccga tcgcctgctc gagcagcgcc gcggtctcgg cgtcgaccgc gttgcgcacc
     4501 tcgggccggt tgagcgtgag cgtccagacc gcaccctcgc gctcgacctc caccttcgcc
     4561 atcgcgtcct cctctgtggt gcgcactagt atccggggcc gtggccacgc tgatccgccc
     4621 gcccggtccc gaccgcgccg acctgcggga ac
//
LOCUS       DASPWO010000263         6494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_39114, whole genome shotgun sequence.
ACCESSION   DASPWO010000263 DASPWO010000000
VERSION     DASPWO010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39114"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VEO00_05920"
     CDS             <1..667
                     /locus_tag="VEO00_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HYU57570.1"
                     /translation="HEIVASADLAARLGVDRPRYALIDPAPGIGRPELAPLLRGLLPP
                     GTPLRLRAPGETPYFRQGDAVLPPVRLKELFGEFAARPAGADGAIAVDTAWEARYIVH
                     RRVPVIGAITCNRVLVPQLSAALAEIERRGLAHLLDPADVGGCYAPRFLSRDPGAGIS
                     HHAWGAAVDLNVSTNPLGRIPTMDARVVEVFERWGFTWGGRWLVPDGMHFDAIRLAPD
                     AGA"
     gene            complement(702..2411)
                     /locus_tag="VEO00_05925"
     CDS             complement(702..2411)
                     /locus_tag="VEO00_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU57571.1"
                     /translation="MSETNVGERLQAGADGALGLTNAYLQALHETSLGLMKRLDLDEL
                     LQAIVRRAATLANTEHGWVYLVEPGTDELEVKVGIGDFASYVGFRMRRGEGLAGKVWE
                     NGETIRLEDYEGWMGRSPNMERGVVRACVGVPLVSGGEVAGVIGLAHVEPGPVFGDEQ
                     VETLTRFAQLASIALDNARLYRAAQQELADRRRTEVALRVSEGRYQRLFESAPTPMWV
                     TDVATQRFLAVNDAAVARYGYSREEFLGMGLLDVRPADDVPALRQVLADPMLREARRR
                     GRHRAKDGTIFDVDVAWQVVEFGGRTARLALITDVTDRLRVEEDRRRAGEELAHALDQ
                     EREAARRLRALDEMKNTFLSAVSHELRTPLTSVLGFALTLEREPNGDAERRAEVLGRL
                     ASNARKLERLLADLLDLDRLAHGSVELRRRPADLAALARRVLEQVELPPDRTVHLDAH
                     QVTVPVDAAKIERVVENLVVNAVRYAPDNSPIWVRVRPEPGGAVVIVEDAGPGIPQEL
                     RESILEPFVQGPAVQPHSPGVGIGLSLVARFTALHDGTVTVGERDGGGASIRVFLPAE
                     PEG"
     gene            complement(2645..3442)
                     /locus_tag="VEO00_05930"
     CDS             complement(2645..3442)
                     /locus_tag="VEO00_05930"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYU57572.1"
                     /translation="MEAAFFDLDKTIISKSTSLALTRPFYRAGLVTRSMLLKGVYAQL
                     VYLLVGADEKKMDRAKEGMLALSKGWDKAQVEEVVRRALIDVVDPYVYQEALDLIALH
                     RALGRRVFIVSSSPEEVVRPLAEHVGVRDVIATRAEIEDGRYTGRLAFYCYGENKAVA
                     VKETAEGEGIDLSVSYAYSDSITDLPMLEEVGNPVPVNPDRELRKEAEKRGWQVRDFR
                     RPVPLRARIKVPRPSRSATAVVAGAGIAAILGWVLLRARLNQRRARD"
     gene            3496..3954
                     /locus_tag="VEO00_05935"
     CDS             3496..3954
                     /locus_tag="VEO00_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57573.1"
                     /translation="MRIESRLEEWNPARIRAALAGLPAADGYRLVVKPLRYRTRPHLT
                     AYCSFDDHAIVVQVPEPWRAFWERVPYRAKRIRGRGFRFRWEFRDVRFRTKREVIRFL
                     YLHEWYHWYLRERLGKKSAAETACDRFALAHFRRRTPIEIPEGAATARIG"
     gene            complement(4247..4750)
                     /locus_tag="VEO00_05940"
     CDS             complement(4247..4750)
                     /locus_tag="VEO00_05940"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HYU57574.1"
                     /translation="MAIFEPILAALNDAEVRYVVVGGVAVVLHGYARLTAALDLAVDL
                     SPREARKVVAALRGVGLGPSAPVDPDGFGDAEVRARWISEKDMRVFSMRDPIDPMRAV
                     DLFVEEPVPFEELWGRSEVIDLGGLPVRVASVEDLIRMKRGVDRPQDRADVAALEAIR
                     ERREGSS"
     gene            complement(4780..5520)
                     /locus_tag="VEO00_05945"
     CDS             complement(4780..5520)
                     /locus_tag="VEO00_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HYU57575.1"
                     /translation="MTPETSETGTGLSRRTLAEETLIVLGLTFLASAAYAIVSLLSAP
                     IRGVSVAVFREVGLERQVLDIVFGLVPVWLVVYLVRRSGEGTAGIGLAWDEPRRDLGR
                     GAVLALLVATVGLGVYVLAVEIGLNRIVIPVPPTGHWWTVPILALGAFQNGLLEETIV
                     AAYLLHRLRQQGWSDPAALWSSAVLRGAYHLYQGFGGFTGNLALGLFFGAYYQRKRRT
                     WPLVLAHTLVDLLAGLGYLLLRDRASFI"
     gene            complement(5606..5839)
                     /locus_tag="VEO00_05950"
     CDS             complement(5606..5839)
                     /locus_tag="VEO00_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57576.1"
                     /translation="MDEPVRLGIAGPAGRGAGRSPERVEPGAVGEVSGVAETLARRSF
                     LYDDPGAFRAGIAETLRALRGAGLLHPSTPTAS"
     gene            complement(6112..>6494)
                     /locus_tag="VEO00_05955"
     CDS             complement(6112..>6494)
                     /locus_tag="VEO00_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYU57577.1"
                     /translation="AELTAVMRTGRVTAVQVPYNPRERAVEREVLPLAAELGIGVVVM
                     RPFGEGSLTRRPPSEADLAPLAPFGVRTWAQALLKWILSDPRCQTAIPATSRLGRTRE
                     NAAAGDPPWFGPEERELVARLAVP"
BASE COUNT          917 a         2390 c         2287 g          900 t
ORIGIN      
        1 ccacgagatc gtggcctcgg ccgacctggc cgcgcgcctc ggcgtcgacc ggcctcgcta
       61 cgcgctgatc gacccggccc cggggatcgg gcggccggag ctcgccccgc tgctgcgcgg
      121 cctcctcccc cccggaacgc cgctgcgcct tcgcgcgccc ggcgagaccc cgtactttcg
      181 ccagggcgac gcggtgctgc cgccggtccg gctgaaggag ctgttcggcg agttcgccgc
      241 caggccggcc ggggcggacg gggcgatcgc ggtggacacg gcctgggagg cgcgctacat
      301 cgtccacagg cgggtccccg tgatcggcgc gatcacctgc aaccgggtgc tcgtccccca
      361 gctctcggcg gcgctcgccg agatcgagcg ccgcggcctg gcccacctgc tcgacccggc
      421 cgacgtcggc ggatgctacg ccccccggtt cctgtcgcgc gacccgggcg ccggaatctc
      481 ccaccacgcc tggggcgcgg ccgtcgacct gaacgtgtcg accaacccgc tcgggcggat
      541 cccgacgatg gacgcccgcg tggtcgaggt cttcgaacga tggggcttca cctggggcgg
      601 ccgctggctg gtgcccgacg gcatgcactt cgacgccatc cgcctggccc cggacgccgg
      661 cgcctgacgg ccgccggccg ggcgccgccg ggtgggccgc ctcagccctc aggttcggcc
      721 ggcaggaaca cccggatcga ggccccgccg ccgtcgcgct cgccgacggt caccgtcccg
      781 tcgtgcagag cggtgaagcg ggccacgagc gagagcccga tcccgacgcc gggcgagtgg
      841 ggttggacgg cggggccctg gacgaacggc tcgaggatcg attcccgcaa ctcctggggg
      901 atccccgggc cggcgtcctc gacgatcacg acggcgccgc ccggctccgg ccggacccgg
      961 acccagatgg gcgagttgtc cggcgcgtag cgcacggcgt tgacgacaag gttctcgacc
     1021 acccgctcga tcttggccgc gtcgaccggc accgtgacct gatgggcgtc gaggtggacg
     1081 gtccggtccg gtggcaactc cacctgctcc agcacgcgcc gggccagggc cgccaggtcc
     1141 gcaggccgcc ggcggagctc gacgctgccg tgcgccaggc ggtcgaggtc gagcaggtcg
     1201 gccaggagcc gctccagctt gcgggcgttc gaggccagac ggccgagcac ctcggcgcga
     1261 cgctcggcgt cgccgttcgg ctcccgctcg agggtcaggg cgaacccgag gacggaggtc
     1321 agcggcgtgc gcagctcgtg cgagaccgcg gacaggaagg tgttcttcat ctcgtcgagc
     1381 gcgcgcagtc gccgggccgc ctcccgctcc tgatccaggg cgtgggccag ctcctccccg
     1441 gcccgccggc ggtcttcctc gacgcgaagc cggtcggtga cgtcggtgat cagggccagg
     1501 cgggccgtcc ggccgccgaa ctcgacgacc tgccaggcca cgtccacgtc gaatatcgtg
     1561 ccgtccttcg cccggtggcg accccggcgc cgcgcttcgc gcagcatcgg gtcggcgagc
     1621 acctggcgca gggcgggaac gtcgtcggcc ggacggacgt ccaggagtcc catccccagg
     1681 aactcctcgc gggagtaacc gtagcgagcc accgcggcgt cgttcaccgc caggaaccgc
     1741 tgggtggcga cgtcggtcac ccacatcggc gtgggggccg actcgaacag ccgctggtag
     1801 cgcccctcgg aaacgcgcag ggccacttcg gtccgccggc ggtcggccag ctcctgctgg
     1861 gctgcgcgat agagacgcgc gttgtccaga gcgatcgagg cgagctgcgc gaagcgggtc
     1921 agcgtctcca cctgctcgtc cccgaacacc gggcccggct cgacatgcgc cagcccgatc
     1981 accccggcca cctcgccgcc ggagacgagc gggacgccga cgcaggcccg cacgacaccg
     2041 cgctccatgt tcggcgagcg gcccatccac ccctcgtagt cctcgagtcg gatcgtctcg
     2101 ccgttctccc agaccttccc ggccaggccc tcgccgcggc gcatccggaa cccgacgtag
     2161 gaggcgaagt cgccgatgcc caccttgacc tcgagctcgt cggtgcccgg ctcgaccagg
     2221 tagacccagc cgtgctcggt gttcgcgagc gtcgctgccc ggcgaacgat ggcctgcagc
     2281 agctcgtcga ggtcgagccg cttcatcagg cccaggctcg tctcgtgtag ggcctggagg
     2341 taggcgttcg tcagcccgag cgcgccgtcc gctcccgcct gcaggcgctc cccaacgttc
     2401 gtctcgctca tctcgtcgat ccgggctccg cggatccctg gacagggata tcggacggat
     2461 cggggaccag ccttagcccg aacggcccac tcggggaccc gaaatctcgg tggcgtagcg
     2521 aagggaacgg ctaccagagc gctctgggtt cgcgttcggg gaccggcccg aaaggttggg
     2581 accgctcctc gaacctgcct cccgcggcag tccgagcgtg ggtaccgctc ccttcggctc
     2641 gatcctagtc ccgcgctcgg cgctggttca agcgggcccg gagcaggacc cagcccagga
     2701 tcgcggcgat gccggcgccg gccacgaccg cggtggccga ccggctcggg cgggggacct
     2761 tgatccgcgc ccgcagcggg accgggcgcc ggaagtcgcg cacctgccag ccccgcttct
     2821 ccgcctcctt gcgcagctcg cggtcggggt tgaccgggac ggggttcccg acctcctcga
     2881 gcatcggcag gtccgtgatc gagtccgaat aggcgtagga gacggacaga tcgatcccct
     2941 ctccctcggc cgtctccttg accgccaccg ccttgttctc gccgtagcag tagaaggcga
     3001 gcctgcccgt gtacctgccg tcctcgatct cggctcgggt ggcgatgacg tcgcgcacgc
     3061 cgacgtgctc ggcgagcggg cgaaccacct cctccggcga ggaggagacg atgaacaccc
     3121 ggcgcccgag cgcgcggtgc agcgcgatca ggtcgagggc ctcctggtag acgtacgggt
     3181 cgacgacgtc gatcagcgcc cggcggacga cctcctccac ctgggccttg tcccagccct
     3241 tggacagggc gagcatgccc tccttggccc ggtccatctt cttctcgtcc gccccgacca
     3301 gcaggtagac cagctgggcg tagacgccct tcaggagcat ggaccgggtg accaggccgg
     3361 cgcggtagaa gggcctggtc agcgccaggc tggtcgactt cgagatgatc gtcttgtcga
     3421 ggtcgaagaa cgccgcctcc atgtcgctcg atggtacgct acgtgtcacc accggccggg
     3481 atactgtcgc ccgtcatgag gatcgagtcg cggctggagg agtggaaccc ggcccggatc
     3541 cgagccgcgc tggccggcct gcccgcggcc gatggctacc ggctcgtggt caagcccctg
     3601 cgctaccgga cgcgccccca cctgaccgcg tactgcagct tcgacgacca cgcgatcgtg
     3661 gtccaggtcc ccgagccgtg gcgggcgttc tgggaacggg tcccctaccg ggccaagcgc
     3721 atccgcggcc ggggcttccg cttccgctgg gagttccgcg acgtccgctt ccgaaccaag
     3781 cgcgaggtga tccgcttcct ctacctgcac gagtggtacc actggtacct gcgggagcgc
     3841 ctgggcaaga aatccgccgc cgagaccgcc tgcgaccgct tcgcgttggc ccacttccgc
     3901 cgccgaaccc ccatcgagat accggaaggc gccgcaacgg cccggatcgg ctgacgtcga
     3961 gaaggatggc gatatcgtca agctggccat cgagatgcag aggggtgacg tactggagat
     4021 cctcggttct aacgtccaga tctgaatcct tgtgcggggc cctgcggggc tcggcgtgtc
     4081 tcgggtcggg ggaaggcgcc ggttcggtag gcgagggcga tcgcctcctc gagccaggcg
     4141 aggcgctggg cgggcgtcgc gtcgagccag cgggtcaggg cctcgcggcg gtgctgctcc
     4201 catccatcat cgtcggacaa cgagtccttg ggcaccgcgt ggtccgtcaa ctgctcccct
     4261 ctcgccgctc tcggatcgcc tccagggccg ctacgtcggc ccgatcctgc gggcggtcca
     4321 ctccacgttt catcctgatg agatcctcga ccgaggcgac gcggacggga aggccgccaa
     4381 ggtcaatgac ctcggaccgc ccccaaagct cttcgaacgg aaccggctcc tccacgaaca
     4441 ggtccaccgc gcgcatcggg tcgatcggat cgcgcatcga gaacacgcgc atgtccttct
     4501 ccgagatcca cctcgcgcgg acctccgcat cgccgaatcc gtcgggatcg accggcgcgg
     4561 acggtcccag gcccacgcct cgcaacgccg ccaccacctt ccgtgcttca cggggagaga
     4621 gatcgacggc cagatcgagg gcggccgtga gcctggcata cccgtggagc accacggcca
     4681 cccctccgac caccacgtac cggacctcgg cgtcgttcag cgcggccagg atcggttcaa
     4741 agatggccac cggacgagtc tatcggtgcg atctgctgct cagatgaacg acgcgcggtc
     4801 gcggaggagc aggtagccga ggccggccag caggtcgacc agggtgtggg cgagcaccag
     4861 gggccaggtc ctgcgcttgc gctggtagta ggcgccgaag aacaggccga gcgccaggtt
     4921 gccggtgaag ccgccgaacc cctggtacag gtggtaggcg ccgcggagga cggcgctcga
     4981 ccacagggcg gcgggatccg accacccttg ctggcgaagt cggtggagca ggtacgccgc
     5041 gacgatcgtc tcctcgagca ggccgttctg gaacgcgccg agggcgagga tcgggaccgt
     5101 ccaccagtgg ccggtcgggg ggaccgggat cacgatccgg ttcaggccga tctcgacggc
     5161 cagcacgtac acgccgagcc cgacggtggc cacgagcagg gcgagcaccg cgccccgtcc
     5221 gaggtcgcgc cgaggctcgt cccacgccag cccgatcccg gcggttccct cgccgctgcg
     5281 gcgcaccagg tagacgacca gccacaccgg gaccaggccg aacacgatgt ccaggacctg
     5341 gcgctccagc ccgacctcgc ggaagaccgc caccgacacg ccccggatcg gcgccgagag
     5401 gaggctgacg atggcgtaag cggccgacgc caggaacgtc agcccgagga cgatcagcgt
     5461 ctcctcggcc agtgtccgcc gcgagaggcc cgtgccggtt tcggaggtct cgggagtcac
     5521 gcagggaact gtacggcggc ctgatgggct accgcggcgt ggtacacggg tcaccggccg
     5581 gccgggaaga gcgcgggcag gtccttcagc tggccgtcgg ggtcgacggg tgcagcaggc
     5641 cggcgcccct gagtgcccgg agcgtctcgg cgatcccggc gcggaaggct cccgggtcgt
     5701 cgtagaggaa ggagcgccgg gcgagtgtct ccgccacgcc actcacctcg cccaccgctc
     5761 cgggctcgac gcgttcgggc gaccgcccgg caccccggcc ggccggtccc gcgatcccca
     5821 gtctcacggg ctcgtccatg gctgcccccc ttccgtggct gccttcccgg tcggacgccc
     5881 gcagcatgac gggcgcgtat aacgccgtcg ttgccgctcg atgacgtccc ggtgaacgat
     5941 ccgtccaccc tttctccggg attcgaccca ccctagaggc cggttcgcgc cggtgaccgg
     6001 cgttcgccgg cttctcagga agcgttcagg gttctcgggc ggccacggcc tcggtcccgg
     6061 ttcgcggggc cgtgcagccc ggtggtggtg ggaacggctc gtgcccggcg ctcagggcac
     6121 ggccaggcgg gccaccagct cgcgctcctc cggcccgaac cagggagggt cgccggcggc
     6181 ggcgttctcg cgcgtccggc cgagccgcga cgtcgccggg atcgccgtct ggcagcgcgg
     6241 atcggagagg atccacttca gaagggcctg ggcccaggtc cggacgccga agggagcgag
     6301 cggggcgagg tcggcctcgc tcggcggccg gcgcgtcagg ctcccctcgc cgaaggggcg
     6361 catcacgacg accccgatcc cgagctctgc cgccagcgga aggacctcgc gctcgacagc
     6421 gcgctcgcgc gggttgtagg ggacctgcac ggcggtgacc cggccggtgc gcatcacggc
     6481 cgtcagctcg gcga
//
LOCUS       DASPWO010000264         4971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_460903, whole genome shotgun sequence.
ACCESSION   DASPWO010000264 DASPWO010000000
VERSION     DASPWO010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460903"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..708)
                     /locus_tag="VEO00_05960"
     CDS             complement(40..708)
                     /locus_tag="VEO00_05960"
                     /inference="COORDINATES: protein motif:HMM:NF025166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HYU57578.1"
                     /translation="MDEAADVDPIAEILAALAYGERAAAARARANVDLAPDGRARSQQ
                     RMTADRERENADLVEARLEERADPGLIERFRPFFDAFFEHTEPADWLEAQTFHYVGDA
                     LVSDFADALMPLLDPVSATVVRSTIADRSAQASFAIDELGRLMEVDPTARDRIAAYAR
                     RIAGEALTQTRRALQQTRVLRSLMGGADELEKRMVLDLLDRHRQRLDRLGIESVDEPA
                     DEGG"
     gene            1049..1321
                     /locus_tag="VEO00_05965"
     CDS             1049..1321
                     /locus_tag="VEO00_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57579.1"
                     /translation="MGRVIDLEAWREARETAARSGDPAVERLDRAMATLEARVEEGGR
                     LSARHETDLLAITGAIALGMIEEAAERLERLAVRMAHPAGTGDRTG"
     gene            1419..2012
                     /locus_tag="VEO00_05970"
     CDS             1419..2012
                     /locus_tag="VEO00_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57580.1"
                     /translation="MRRVVLAACAFGWLLAFAQPSRAGGGGGEICQPHRTRPGLLTVF
                     VTDNCFTYKTLRVTTGSAVTWRLLGTFAPHTVSAENGLFESGDLSGPFTVRFNQPGRY
                     PYVCTYHGGSWGGMRGTVVVTGAAVDGPEAEELVAESEVDEASARAVVPAPVVTVEVP
                     AAGPVRASLSGPTTLALVVGMFASVLALAFLLRRGRA"
     gene            2046..2567
                     /locus_tag="VEO00_05975"
     CDS             2046..2567
                     /locus_tag="VEO00_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYU57581.1"
                     /translation="MQEPDPALIRRAQQGDMDAFAELVRACQADCWRFARSLLHDRHA
                     ADDVTQEAFLRAYRFLGSYRGESRFSTWLFRLVHNCAMDEVRRIERARRPGPAPAPGT
                     DVHARAEIREALDALPAALREPFLLVEVFGLRYADSAAVLGVKEGTVKSRVHRARAAL
                     IASLTVREEADGL"
     gene            2557..4506
                     /locus_tag="VEO00_05980"
     CDS             2557..4506
                     /locus_tag="VEO00_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57582.1"
                     /translation="MDCRAVRPALSARMDGVARGLDPAAEAHLAACAGCRAFDAAAWR
                     LREAVRFQVAEPVPDLVEAIMDRVRTLPDRPRPLRLTPRRRDRPRNLTPVRSLVAAAL
                     AGALAGALVVGAGLAPRPSPAALATEIPGLIAAAATGVDEYAATFRIEERNWRPEVPA
                     RTFDVDVAFAAPERLRVRVADRTAYPAGPWPRNDSLLVVVGSRWELTGPEACTAEAAP
                     ACGRTPDVRRAVTGRAPFDADAPMPTDIVLPVTTLAGSERVTVLGEGRAAGRDAVRVE
                     LAAADGGPLFAFFQEAGSWRPQYPADRVTLWLDRETWLPLRFTVAAVASPDRDAWAAA
                     NGIPREPAGSIVLQARAVRLSLGPVDRKRFAVSGGTGRDQGFRALGPVGLARALGADP
                     IQPADTGSLRPLRAGRFAGRHGEVLLSYGDGLARMTVAETRTHRGPSFFGNVGPLAER
                     VRLPGGGVAYYEPAVGTAGRRLAVHAAGWDLFLESNLPRAELLAVAGSLPVRGVAVPA
                     SWDVRTGPGGLRVERVGLSEAARRAGFPVLVPSPAPDGFELASVDLVELSTGRGVTLY
                     YRRAGAEPGGYALRLHQARGEDLPPASGTDQVAVRVAGALGRWSPGRHELEWVGGGIY
                     RSLAAPGLELERILAVAGSLAEVAR"
     gene            4503..>4971
                     /locus_tag="VEO00_05985"
     CDS             4503..>4971
                     /locus_tag="VEO00_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57583.1"
                     /translation="MRRSGPMLAAGAAGMMAGALLTLALQGGASPAEPGAPPAPSMPS
                     PVPRTPEPPGAILAWTPGRLPDGFGAAVLRLPDVEGVAVVASGTAWLSASWSADGGPV
                     DRPPDGLAIPLELAGVDALGYGRFLPPAERGVQAALAGGEVALGASSARLRGLG"
BASE COUNT          531 a         1861 c         1914 g          665 t
ORIGIN      
        1 agagtccatg ctccaccgcg tgagctaccc gagagcccct cagccgccct cgtcggcggg
       61 ctcgtcgacg gactcgatgc cgagccggtc caagcgctgc cgatggcggt cgaggagatc
      121 gaggaccatc cgcttctcca attcatcggc accgcccatc agcgacctga gcacccgggt
      181 ctgctggagc gcccggcggg tctgggtgag cgcctctccg gcgatccgcc gggcgtacgc
      241 cgcgatgcgg tcccgggcgg tggggtcgac ctccatcagc cggcccagct cgtcgatggc
      301 gaacgaggcc tgcgccgagc ggtccgcgat cgtggagcgg acgaccgtgg cggagaccgg
      361 gtcgagcagg ggcatcagcg cgtcggcgaa atcggagacg agcgcgtcgc cgacgtagtg
      421 gaaggtctgc gcctcgagcc agtccgccgg ctcggtgtgc tcgaagaagg cgtcgaagaa
      481 cggccggaac cgctcgatga gcccggggtc ggcgcgctcc tcgaggcggg cctcgaccag
      541 gtcggcgttc tcccgctctc ggtcggcggt catccgctgc tgcgagcgtg cccggccgtc
      601 gggcgccagg tccacgttgg cccgggcgcg ggcggccgca gcgcgctcgc cgtacgcgag
      661 cgccgcgagg atctcggcga tgggatcgac gtccgcggcc tcgtccacct gcagcgcatt
      721 gtaggggccg ccctcgcgga agccgccggg taccattcgc tgcgaagggc gtgatcgcga
      781 cagtcttcct ggaatggctc ctgtcgatcg gcgccgccat cgtcgcgctc gcgttcgcca
      841 tgtacgtcgc tgcgttcacg gccgggttcc tgggccgctc ccgagctcga gcggctgcgc
      901 ccgcgcacgg gctgggcggc cgcgcgcgtg gcccgccggg ctccggtgag cccctgacct
      961 gcggttttgc cgctccggcc caggcctgct cgcctggccg ggctcgaaat cccaaacgtg
     1021 tcagaccccc ccggtaccat tcggctcgat gggcagggtc atcgacctcg aggcgtggcg
     1081 ggaggcccgg gaaacggccg cccggtccgg cgatcccgcg gtcgagcggc tcgaccgggc
     1141 catggccacg ctcgaggccc gcgtcgagga gggcggccgg ctctccgcac ggcacgagac
     1201 cgacctgctc gccatcaccg gcgccatcgc cctcgggatg atcgaggagg ccgccgagcg
     1261 cctcgaacgc ctggccgtgc gcatggccca ccccgccggc acgggcgacc gcaccggctg
     1321 agcgcggttc atccggtcac ccggcgacgg tccccgggcg aaccaatcgc cctgccggac
     1381 ggtccgatca gcggactcga aggaaggggg gaccgcccat gcgccgtgtc gtgcttgccg
     1441 cctgcgcgtt cggctggctc ctggcgttcg cgcagccgtc acgggccggc ggcggcggcg
     1501 gagagatctg ccagccgcac cgcaccaggc cgggactgct gacggtgttc gtgaccgaca
     1561 actgcttcac gtacaagacc ctgcgcgtca cgaccgggtc ggcggtcacg tggcggctcc
     1621 tcgggacgtt cgcgccgcac acggtctccg ccgagaacgg gctcttcgag agcggagacc
     1681 tgagcgggcc gttcaccgtc cgcttcaacc agccgggccg ctacccctac gtgtgcacct
     1741 atcacggagg ctcgtggggt gggatgcgcg ggaccgtcgt cgtgacgggc gcggcggtgg
     1801 acgggccgga ggccgaggag ctggtggccg agagcgaggt cgacgaggcg tcggcacggg
     1861 cggtggtccc ggcgccggtg gtcacggtcg aggtgccggc cgccgggccg gtgcgggcgt
     1921 cgctgtccgg gccgacgacg ctcgcgctgg tggtcgggat gttcgcgtcg gtcctggcac
     1981 tggccttcct cctgcgccgt ggccgggcgt aggctccggg tccggctccc ggcgtgggag
     2041 agaggatgca ggagcccgac ccggcactca tccgcagggc gcagcagggc gacatggacg
     2101 ccttcgccga gctcgtgcgg gcttgccagg ccgactgctg gcgattcgcg cggtcactgc
     2161 tccacgaccg ccacgcggcc gacgacgtca cccaggaggc gttcctccga gcgtaccggt
     2221 tcctgggctc ctatcgcggc gagtcccgct tctccacctg gctgttccgg ctggtccaca
     2281 actgcgcgat ggacgaggtg cggcgaatcg agcgggcgcg ccgtccgggc ccggcaccgg
     2341 cgccgggcac cgacgtccac gcccgggccg agatccgcga ggccctcgac gcgctccccg
     2401 cggccctgcg cgagccgttc ctgctggtcg aagtgttcgg gctgcgctac gcagactcgg
     2461 ccgccgtgct cggggtgaag gagggaaccg tgaagagccg cgtccatcgg gcgcgcgcgg
     2521 ccctgatcgc gtccctcacg gtgcgggagg aggccgatgg actgtagggc cgtgcggccg
     2581 gcgctctcgg cgcggatgga cggcgtggcg cgcggcctcg accccgccgc cgaagcgcac
     2641 ctcgcggcgt gcgccgggtg ccgcgccttc gatgccgcgg cctggcgcct gcgcgaggcg
     2701 gtccgcttcc aggtggccga accggtcccc gacctggtcg aggcgatcat ggaccgcgtc
     2761 cggacgcttc ccgaccggcc tcgccccctg cggctcaccc ctcgccggcg ggatcggccg
     2821 cggaacctga ctcccgtgcg ctcgctcgtc gccgcggccc tggccggggc cctggctggc
     2881 gcgctggtcg tgggtgccgg cctggcgccc cggccgtccc cggcggcgct cgcaaccgag
     2941 atccccggcc tgatcgccgc cgccgcgacg ggggtcgacg aatacgcggc gaccttccgg
     3001 atcgaggagc gcaactggcg ccccgaggtc cccgcgcgga cgttcgacgt cgacgtcgcc
     3061 ttcgcggcgc cggagcggct gcgcgtccgg gtggccgacc ggacggcgta cccagccggt
     3121 ccctggccgc gcaatgattc cctgctggtc gtggtcggct cgcgctggga gctgaccggg
     3181 ccggaggcgt gcaccgccga ggcagcgccg gcgtgcgggc ggacgccgga cgtccggcgg
     3241 gcggtgaccg gacgggcccc gttcgacgcc gacgcgccga tgcccaccga catcgtcctc
     3301 ccggtgacca cgctcgccgg gtccgagcgg gtcaccgtcc tcggcgaggg ccgcgccgcc
     3361 ggccgcgacg ccgtgcgcgt cgagctggcc gccgccgacg gcggtccgct gttcgccttc
     3421 ttccaggagg ccggctcgtg gcggccacag tacccggccg accgcgtcac gctctggctg
     3481 gaccgcgaga cctggcttcc gctgcgcttc acggtcgccg cggttgcgag ccccgaccgc
     3541 gatgcctggg ccgcggcgaa cggcatcccc cgcgagcccg ccggctcgat cgtcctccag
     3601 gcgcgggcgg tccggctgtc tctcggaccg gtcgaccgga agcggttcgc cgtctccggc
     3661 gggaccggcc gcgaccaggg gttccgcgcg ctcggaccgg tggggctggc gcgagcactc
     3721 ggcgccgacc cgatccagcc cgcggacacc gggagccttc ggccgctccg cgccggccgc
     3781 ttcgccggcc gtcacgggga ggtcctgctg tcctacggcg acggcctggc ccggatgacc
     3841 gtcgccgaga cccgcacgca tcgcgggccg tcgttcttcg gcaacgtcgg cccgctcgcg
     3901 gagcgggtcc ggctgcccgg cgggggggtc gcgtactacg agcccgccgt cgggacggcc
     3961 ggccggcggc tggccgttca cgcggccggg tgggacctgt tcctggagtc gaacctgccg
     4021 cgcgcggaac tgctcgccgt ggccgggtca ctcccggtgc gcggcgtggc cgtccccgct
     4081 tcgtgggacg tgcgcacggg gcccgggggc ctgcgggtcg agcgggtcgg gctgagcgag
     4141 gccgcccgcc gggccggctt cccggttctg gtgccgagcc ccgccccgga cgggttcgag
     4201 ctcgcgtccg tggacctggt cgagctctcc accggcaggg gcgtgacgct ctactaccgg
     4261 cgcgccggag cggagccggg cggctacgcc ctgcggctgc accaggcccg tggcgaggac
     4321 ctcccgccgg cgagcggcac cgaccaggtg gccgtccggg tggccggcgc tcttgggcgc
     4381 tggtcgcccg ggcggcacga gctggagtgg gtcggcgggg gcatctaccg ctcgctggcc
     4441 gcgcccggcc tggagctgga gcggatcctg gcggtggccg gatcgctcgc ggaggtggct
     4501 cggtgaggcg atccggcccg atgctcgcgg cgggggcggc cggcatgatg gccggcgccc
     4561 tgctgacgct ggccctgcag ggtggggcga gccccgccga gcccggggca cctcccgcgc
     4621 cctccatgcc ctcccccgtg ccgcggaccc ccgagccgcc aggggccatt ctggcctgga
     4681 cgccggggcg gctcccggac ggcttcggcg ccgcggtcct ccgcctgccg gacgtcgagg
     4741 gcgtcgccgt cgtcgccagc gggacggcct ggctgagcgc ctcgtggagc gcggacggcg
     4801 gcccggtcga ccggccgccg gacggcctgg ccatcccgct cgagctggcc ggcgtggacg
     4861 cgctcggcta cggacggttc ctgccgccgg ctgagcgcgg cgtgcaggct gccctggccg
     4921 gcggcgaggt ggccctcgga gcatcgtccg cgcgactgcg aggcctgggg c
//
LOCUS       DASPWO010000265         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1224431, whole genome shotgun sequence.
ACCESSION   DASPWO010000265 DASPWO010000000
VERSION     DASPWO010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224431"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VEO00_05990"
     CDS             <1..540
                     /locus_tag="VEO00_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018805147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYU57584.1"
                     /translation="VDPERDHVRGPQDAAVTVVEYGDFQCPYCGRAEPAVRAILGDTD
                     VRFIWRHLPLTDVHPDAELAAQAAEAAGAQGGFWEMHDLLLAHQDHLRKPDLLAYAEQ
                     LGLDVPRFHKELYSHAHLARVGQDVESADISGVSGTPTFFVNGRRQYGAFDAASLTSA
                     VAAARARNRIRPTRGRDES"
     gene            1258..1482
                     /locus_tag="VEO00_05995"
     CDS             1258..1482
                     /locus_tag="VEO00_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57585.1"
                     /translation="MGERDAVIEPEEIGGLVLRRLVPPRTTRLRTRAAITSVDLVHRL
                     LDDLASGIPVDGHNSIVAADGEHRVQGGPT"
     gene            1566..2018
                     /locus_tag="VEO00_06000"
     CDS             1566..2018
                     /locus_tag="VEO00_06000"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYU57586.1"
                     /translation="MPERHITVQRRMSASTGSVWALYADFPNLASHWSGLRATRAIGD
                     QTSGVGARRRVELKPIGNMEETVTTWEEGRRIDTRNQPSVSVPFKQAESTLTLEPDDD
                     GTLATFDYRYVPRGGPLGRLTGPLIDKMLTATFTDMLAATEKAALANG"
BASE COUNT          397 a          849 c          798 g          378 t
ORIGIN      
        1 gtcgatccgg agcgggacca cgtgcgtggt ccgcaggacg cggcggtgac cgtggtcgag
       61 tacggcgact tccaatgccc ctactgcggc cgggccgagc cagcggtgcg cgccatcctc
      121 ggcgataccg acgtgaggtt catctggcga cacctgccgc tcaccgacgt gcatcccgac
      181 gccgagcttg cggcgcaggc agccgaggcc gccggcgccc aaggcgggtt ctgggagatg
      241 cacgacctgc tgctcgccca ccaggaccac ctgcggaaac ccgatctgct cgcgtacgcc
      301 gagcagctcg gcctggacgt gccgcgcttc cacaaggagc tgtactcgca cgcgcacctc
      361 gctcgcgtgg gacaagatgt tgagtccgcg gacatcagcg gtgtctccgg gacgccgacg
      421 ttcttcgtca acggccgccg ccagtacggc gccttcgacg ccgcatcact cacgagtgcc
      481 gtcgcggctg cccgcgcccg caaccggatc cggccgaccc gcggacgcga tgagtcgtga
      541 ccgttcagac cgcgctgcta ccgcgatccg ggcggtagca gcgccgcctc atctagccgc
      601 gagggccgtg gaccaccacc ccggcctcat ctacctagcg tggctggagg ccagagtcca
      661 gttgtcggtt cgggccagct tcgggagtcc tgggtgggca cgctgaaggt tccccacggg
      721 acccggacga ctcatccccg ccacccgaat tgcctattcg gactcgttcc gacgagcagc
      781 accacgcgcg tcgacgtacg ctcactgagc cctcgccggt gacccgagac gccggcgggg
      841 gcaccatcgg ggtcgttcgg gtcgtggggg accccacgcg tcggatcacc aacgcggaac
      901 cgcctcctcg cggagattgc gggaaggctt cacccaccga ccttgccgac ctcgagccaa
      961 gaacgggtcg ttcggcagcc tcccggccca ccaggcggga cgcaagcagc ccccgttcga
     1021 ggccaaatca atccccctgg tcacgaacgg gggctcttca cgtgcctgtg gcggtaagaa
     1081 cggtgtcagc agccgccgtc gccctgtggt ggtcgtcgtc tcagatcgcc gctgtcggcg
     1141 gcggcgccgc aagatctgcc tcgccaatag acattcgcgc tcgtggccga tcgcggagtt
     1201 gcggtgccgg tcaccgctca ccggtaatcg gtcatcgggg gcgccccaac tgtcgcggtg
     1261 ggtgagcgcg acgccgtcat agagcccgag gagatcggcg ggctggtcct tcggcggctc
     1321 gtcccgccga ggacgaccag gctgcgcacc cgcgcggcga tcacctcggt ggatctcgtc
     1381 caccgcctgc tcgatgatct cgcgtccggc atccctgtcg acggccacaa ctccatcgtg
     1441 gcggccgacg gggaacaccg ggtccagggc ggtccaacct gattgcgcgg gcggatctgc
     1501 tggcgcgacc atcggaaggg gcggcgcact gccctacagc cagccggaca cttggagtgg
     1561 tcctcatgcc tgaacgtcac atcacggtgc aacgtcgcat gtctgcgtcc acgggttccg
     1621 tatgggccct gtacgccgac ttcccgaatc tcgcgagcca ttggagtggc ctcagggcaa
     1681 cgagagccat cggcgaccag accagtggcg tgggcgctcg gcgccgcgtc gaactgaagc
     1741 cgatcggcaa catggaagag accgtcacca cctgggagga gggtcgccgg atcgacacac
     1801 ggaatcagcc gagcgtgtcg gtgccgttca agcaggcaga gtccacgctc acgctcgagc
     1861 ccgacgacga cgggacgttg gccacgttcg actaccgata tgtacccagg ggtggcccac
     1921 tcgggcgact caccggccca ttgatcgaca agatgctgac ggcgaccttc accgacatgc
     1981 tggccgccac cgagaaggcc gccctcgcga acgggtagcg agcgctgaac cgccccggag
     2041 cccgaggaac tgcgtgtgtt cactcacggc gccactgagc tcgctgacgg acaggactgg
     2101 aacggtgcgc tcgttgatca tccccctact cggtcagcgc gagggatcgc catccagacg
     2161 tgctccgtag cggccgctat ccgcgcgcta tcgagtgccc gctctgtact ttgcgcagcg
     2221 tgcgcgcaga cggccgatcc gtgcaagggt tccccgcggg cgatagcgaa cccagaaatg
     2281 actcgctctc ttcttagaaa accccttcac cggtggactc tgcctgccac tgcctcgggt
     2341 ctcgggtcag cgcgcgtcac ggcgctgggg ggtggtactt atatgagggc gtcgtcaagg
     2401 actcttgacg cggtgaggca cg
//
LOCUS       DASPWO010000266         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1224570, whole genome shotgun sequence.
ACCESSION   DASPWO010000266 DASPWO010000000
VERSION     DASPWO010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224570"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VEO00_06005"
     CDS             complement(<1..312)
                     /locus_tag="VEO00_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HYU57587.1"
                     /translation="MGDTVTAPVVSVTERAAEKAIELAAREGHARSILRLRVTAGGCS
                     GFQYVLGFEEGTSADDLIHEFHGLTLVVDPKSAPIVEGSTLEFIDAMLGGGFKVQNPR
                     AV"
     gene            370..1143
                     /locus_tag="VEO00_06010"
     CDS             370..1143
                     /locus_tag="VEO00_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYU57588.1"
                     /translation="MDLGLSGRRALVAAGSRGLGRASAEALLAEGARVAICGRDRDAL
                     QAAASEIGAVPIVADVSTVEGALGLVQGGAEALGGCEILVPNSGGPPTARFGDLGDDD
                     WQAAFELLLRSWARMIRAALPHMRAAGYGRVVGIGSSAMKQPIPNLILSNALRAGVAG
                     MLRTLADEIAPEGITVNVVLPGRILTDRVRFMARDRSARSGRPEEDELRAIAGDIPMG
                     RIGDPADVGAMVAYLASERAAYVTGAFVAVDGGLYRGLF"
     gene            1219..1782
                     /locus_tag="VEO00_06015"
     CDS             1219..1782
                     /locus_tag="VEO00_06015"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HYU57589.1"
                     /translation="MALDLRAAVAPHLDARPSVPEGGVPAGVLVPVIEGEEPSLVFTV
                     RADTLSHHGGEVSFPGGRPHDEDPDLRATALRETQEELGIDPGSVEVLGALPPVPTFV
                     SGFVVVPFVGMLPELPALRPNPAEIAEVLEIPVRTLLSVEREVEWLIEGRTWTGHVFE
                     HDGHVIWGATARMLKTFLDAIREEGWE"
     gene            1779..2207
                     /locus_tag="VEO00_06020"
     CDS             1779..2207
                     /locus_tag="VEO00_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HYU57590.1"
                     /translation="MSEWAAPDDRELRELLRSWKTIAMVGASSREDRPSNRVFRYLLS
                     KGYRVIPVNPNETEVEGEPAYPSLAAANEALGEIDVVDVFRRAEDTPPIARQAVEVGA
                     KALWLQQGIYSEEAGRIASDAGLDVVMGICMKTVHQELVG"
     gene            2236..2400
                     /locus_tag="VEO00_06025"
     CDS             2236..2400
                     /locus_tag="VEO00_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019174463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57591.1"
                     /translation="MQQWEYFVAPLLPHNPGEILNSFGDDGWELVTLIEQSTPAGGAS
                     IVAYLKRPKG"
     gene            complement(2410..>3180)
                     /locus_tag="VEO00_06030"
     CDS             complement(2410..>3180)
                     /locus_tag="VEO00_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HYU57592.1"
                     /translation="PAMYRMLEETGAEEADLRTIRLWISGANAMPRGLARRFQRRGSS
                     LAVGSRSLLPAFFVEGYGLVEATGPALFRALGPVGVMRTFRPLPGWRARAVDEDGEPV
                     AHGEPGTLELSGPGVVAGYLGERPRERGWLRTQDLATVSRTQGVEILGRSDDLLKVGG
                     HRLHPGEVEAALLAHPAVADAGVTSVPHRTMGAVPAAGVVLRAGAATRPDELAAFAAK
                     RLASYKMPRRVQVVDAIPRTPTGKIRRGVLRERLSADR"
BASE COUNT          421 a         1130 c         1207 g          422 t
ORIGIN      
        1 cacggcgcgc gggttctgca ccttgaagcc gccgccgagc atcgcgtcga tgaactcgag
       61 ggtcgagccc tcgacgatcg gcgcgctctt ggggtcgacg acgagcgtca gcccgtggaa
      121 ctcgtggatg aggtcgtcgg ccgaggttcc ttcctcgaag ccgagcacgt actggaagcc
      181 cgagcacccg ccggcggtga cccgtaggcg caggatcgac cgggcgtgtc cctcgcgggc
      241 ggccagctcg atcgccttct cggcggcccg ctcggtgacg gacacgacgg gcgcggtgac
      301 ggtgtccccc atggcggttc gatggtatca aagcgccgtg gagtgcctgc tcggcgcgcg
      361 ggaaccgtcg tggacctcgg gctgagcggg cggcgggcgc tcgtcgccgc cggcagccgc
      421 gggctcgggc gggcgagtgc cgaggcgctg ctggccgagg gcgcgcgggt ggcgatctgc
      481 ggccgggacc gcgacgcgct gcaggcggcc gcgtcagaga tcggcgcggt cccgatcgtc
      541 gccgacgtgt ccaccgtcga gggggcgctc gggttagtcc agggcggcgc cgaggcactc
      601 ggcggctgcg agatcctggt cccgaactcg ggcggcccgc cgaccgcccg cttcggcgac
      661 cttggcgacg acgattggca ggccgcgttc gagctcctgc tgcgctcctg ggcccggatg
      721 atccgcgccg cgctgccgca catgagggcc gccgggtacg ggcgggtcgt cgggatcggc
      781 tcgtcggcga tgaagcagcc gatcccgaac ctgatcctgt cgaacgcgct gcgcgccggt
      841 gtcgccggca tgcttcggac gctggccgac gagatcgccc ccgaggggat caccgtgaac
      901 gtggtcctgc ccggccggat cctgaccgac cgcgtccgat tcatggcgcg ggaccgctcg
      961 gcgcggagcg gccgccccga ggaggatgag ctccgggcca tcgccggcga catcccgatg
     1021 ggccggatcg gcgacccggc cgacgtcggc gccatggtcg cctacctggc gagcgagcgc
     1081 gccgcctacg tcaccggcgc cttcgtcgcc gtcgacggcg gcctgtaccg cggcctgttc
     1141 tagccgtggg aaaccatcat caccacgtct cggagggtac ccgacgggcg cgccgcgctc
     1201 ccggcctaga cttccctcgt ggccctcgac ctgcgcgccg ccgtcgcgcc gcacctcgat
     1261 gcgcggccgt cggttcccga gggcggggtc ccggccggcg tcctcgtccc ggtgatcgag
     1321 ggggaggagc cgtcgctcgt gttcacggtc cgggcggaca cgctcagcca ccacgggggc
     1381 gaggtctcgt tccccggcgg ccgcccccac gacgaggacc cggacctgcg cgccaccgcc
     1441 ctgcgcgaga cgcaggagga gctcggcatc gacccgggct cggtggaggt gctcggggcg
     1501 ctgccgccgg tgccgacctt cgtcagcggc ttcgtggtcg tcccgttcgt cgggatgctg
     1561 cccgagctgc cggcgctcag gccgaacccc gcggagatcg ccgaggtgct ggagatccca
     1621 gtgcgcaccc tcctgtcggt ggagcgggag gtggagtggc tgatcgaggg aagaacctgg
     1681 accggccacg tgttcgaaca tgacgggcac gtcatctggg gcgcgacggc gcgcatgctg
     1741 aagacgttcc tcgacgcgat ccgcgaggag ggctgggaat gagcgagtgg gcggcaccgg
     1801 acgaccgcga gctgcgcgag ttgctccggt cctggaagac catcgcgatg gtcggcgcct
     1861 cgagtcgtga ggaccggccg tcgaaccggg tgttccgcta cctgctctcg aagggctacc
     1921 gcgtgatccc ggtcaacccg aacgagaccg aggtggaggg cgaaccggcc tatccgtcgc
     1981 tcgccgcggc gaacgaggcg ctcggggaga tcgacgtggt ggacgtgttc cgccgtgcgg
     2041 aggacacgcc gccgatcgcg cgccaggcgg tcgaggtcgg ggcgaaggcc ctgtggctgc
     2101 agcaggggat ctacagtgag gaggcggggc ggatcgcctc ggacgccggg ctcgacgtcg
     2161 tgatgggcat ctgcatgaag acggtccacc aggagctggt cggctgaccg gcgcaccacg
     2221 gggaggaggt ctccagtgca gcagtgggag tacttcgtgg cgccgctcct tccgcacaac
     2281 ccgggcgaga tcctgaacag cttcggcgac gacggatggg agctggtgac gctcatcgaa
     2341 cagtccacgc cggcgggcgg cgcctcgatc gtcgcctacc tgaagcgccc gaagggctga
     2401 ccggcccggt cagcggtcgg cggacagccg ctcgcggagc acgccacgcc tgatcttccc
     2461 ggtgggcgtc cgggggatgg cgtccaccac ctggacgcgg cgcggcatct tgtaggacgc
     2521 cagccgcttc gcggcgaacg cggccagctc gtcgggcctc gttgccgcgc cggcccggag
     2581 aacgacgccg gcggcgggga ccgcgcccat cgtccggtgg ggcacggagg tgacgccggc
     2641 gtcggccacc gccgggtgcg ccagcagagc cgcctcgacc tcgccggggt ggagccggtg
     2701 tccgcccacc ttcagcaggt cgtcggaccg ccctaggatc tcgacgccct gcgtccgcga
     2761 cacggtggcc aggtcctggg tgcgcagcca tccgcgctcg cgcgggcgct cgcccaggta
     2821 gccggccacc acgccgggac cggacagttc gagcgtcccc ggctccccgt gggcgacggg
     2881 ctccccgtcc tcgtccacgg cccgggcgcg ccagccgggc agcggccgga acgtgcgcat
     2941 cacgccgaca ggcccgagcg cccggaacag cgccggcccg gtcgcctcca ccaggccgta
     3001 cccctcgacg aagaacgccg ggagcagcga gcgcgacccc acggcgagcg aggagcctcg
     3061 ccgctggaac cgacgggcca ggccgcgggg catggcgttg gcgccggaga tccagagccg
     3121 gatcgttcgc aggtcggcct cctccgcgcc ggtctcctcg agcatccggt acatcgcggg
//
LOCUS       DASPWO010000267         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_121587, whole genome shotgun sequence.
ACCESSION   DASPWO010000267 DASPWO010000000
VERSION     DASPWO010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121587"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VEO00_06035"
     CDS             complement(<1..178)
                     /locus_tag="VEO00_06035"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HYU57593.1"
                     /translation="MMVAVVSFDLRIPGSTSLKEKRHVVKALSASLRQRFNVSVAEVD
                     HHDLWQRATVAVSAV"
     gene            complement(188..>2090)
                     /gene="infB"
                     /locus_tag="VEO00_06040"
     CDS             complement(188..>2090)
                     /gene="infB"
                     /locus_tag="VEO00_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HYU57594.1"
                     /translation="PVEATVEAVGRRPRRAKEPEAVAPEPEAEPEPVAVAAPEPEPEE
                     EPGGRRTLKIHRGATAQEIAERTGATAAEIVKTLIMAGEMSTATQPLSDEALALVLDS
                     LGLDGEIVEPEEEEAEEEEAEEVDESRLVGRAPVVTVMGHVDHGKSSILDAIRQTDVV
                     SGEAGGITQHIGAYQVHQNGREITFIDTPGHEAFTAMRARGAQVTDIAILVVAADDGV
                     KPQTLEAIDHARAAEVPIVVAVNKIDKPEADPTRVRQQLSEQGVQPEEWGGDTVFVDV
                     SAKTKDGLEQLLEMISLVADLEDLKADPEAPARGVAIEAHLDRGRGPSATVLVSRGTL
                     RIGDAVVAGTAHGRVRQMLDENGQAVQAAGPSKPVQVLGWSSVPQAGDEVRVVADERE
                     ARRIAQEREAKHRAAEQVAAGRPSSLAELLARAQQGEIPELPIILKADVQGSIEALTE
                     SLEKLPSDEVRVRILHRGTGQIGENDVTLAQASGAIIIGFNVRPSPAARELAEKEGVD
                     VRLYDVIYQAVDDVRAALSGMLAPAEQEVELGRAEVRALFRVPRLGVIAGSYVQGGTI
                     VRSGRVRVVRDGTVVHDGRIASLRRFKDDVREVAEGFECGIGLESFQDLKEGDVLEAY
                     EVREVARSL"
BASE COUNT          251 a          813 c          709 g          317 t
ORIGIN      
        1 tcaccgccga cacggcgacc gtcgcgcgct gccacaggtc gtggtgatcg acctcggcga
       61 ccgacacgtt gaagcgctgc cgcagcgagg cgctcagggc cttgaccacg tgccgcttct
      121 ccttcagcga ggtggacccc ggtatccgga ggtcgaacga caccaccgcc accatcatgg
      181 cggtgcgcta caggctccgg gcgacctcgc ggacctcgta ggcctcgagg acgtcgccct
      241 ccttgaggtc ctggaacgac tcgaggccga tcccgcactc gaagccctcg gccacctcgc
      301 ggacgtcgtc cttgaaccgg cgcagcgagg cgatccggcc atcgtgcacg accgtcccgt
      361 cgcgtaccac ccggacccgc ccgctgcgga cgatcgtccc cccctggacg taggagccgg
      421 cgatgacgcc gagccgcggg acgcggaaca gggcccggac ctcggcccgt ccgagctcca
      481 cctcctgctc ggccggggcg agcatcccgg agagcgcggc gcggacgtcg tccaccgcct
      541 ggtagatcac gtcgtacagg cggacgtcca cgccctcctt ctcggccagc tcacgcgccg
      601 ccggcgaggg ccggacgttg aacccgatga tgatcgcccc cgacgcctgc gccagcgtga
      661 cgtcgttctc gccgatctgc ccggtcccgc ggtgcaggat ccgcacccgg acctcgtccg
      721 agggcagctt ctcgagcgac tcggtgagcg cctcgatcga gccctgcacg tcggccttca
      781 ggatgatcgg cagctcgggg atctcgccct gctgcgcccg ggccagcagc tcggccagcg
      841 aggacggccg gccggcggcg acctgttcgg cggcgcggtg cttcgcctcg cgctcctggg
      901 cgatccggcg ggcctcgcgc tcgtcggcga ccacgcgcac ctcgtccccg gcctgcggga
      961 ccgaggacca tccgagcacc tgcacgggct tggacgggcc ggcggcctga acggcctgcc
     1021 cgttctcgtc gagcatctgg cggacccggc cgtgcgcggt cccggcgacc accgcgtcgc
     1081 cgatgcgcag cgtcccgcga gacacgagta cggtcgcgct cgggccgcgc ccgcggtcga
     1141 ggtgggcctc gatggcgacc ccgcgcgccg gagcctccgg atcggccttc aggtcctcca
     1201 ggtcggcgac cagcgagatc atctccagca gctgctcgag accatccttc gtcttcgccg
     1261 agacgtcgac gaacacggtg tcgccgcccc actcctccgg ctgtacgccc tgctccgaca
     1321 gttgctgccg gacgcgggtc gggtcggcct ccggtttgtc gatcttgttc accgccacga
     1381 cgatcgggac ctccgccgcc cgggcatggt cgatcgcctc gagggtctgc ggcttgacgc
     1441 cgtcgtcggc ggccacgacg aggatcgcga tgtcggtgac ctgcgccccg cgggcccgca
     1501 tcgcggtgaa cgcctcgtgg cccggcgtat cgatgaacgt gatctcgcgc ccgttctggt
     1561 gcacctggta ggcgccgatg tgctgggtga tgccgcccgc ctcgccggac accacgtcgg
     1621 tctggcggat cgcgtcgagg atcgaggact tgccgtggtc gacgtgcccc atcacggtga
     1681 cgaccggcgc ccgtccgacc aggcggctct cgtcgacctc ctcggcctcc tcttcctcgg
     1741 cctcctcctc ctcgggctcg acgatctcgc cgtcgaggcc gagcgagtcg aggaccaggg
     1801 ccagggcctc gtccgagagc ggctgcgtgg ccgtggacat ctccccggcc atgatcaggg
     1861 tcttcacgat ctcggcggcc gtggcaccgg tgcgctcggc gatctcctgg gcggtcgcgc
     1921 cgcggtggat cttcagcgtc cgccgcccgc cgggttcctc ctccggctcc ggctccggcg
     1981 cagccacggc cacgggttcg ggttccgcct ccggctcggg ggccacggcc tccggctcct
     2041 tcgcccggcg cggccgccgg ccgacggctt ccacggtggc ctcgaccggc
//
LOCUS       DASPWO010000268         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_381734, whole genome shotgun sequence.
ACCESSION   DASPWO010000268 DASPWO010000000
VERSION     DASPWO010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381734"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1244)
                     /locus_tag="VEO00_06045"
     CDS             complement(51..1244)
                     /locus_tag="VEO00_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYU57595.1"
                     /translation="MPSDSGGRGWGPPWRHGHGPGYEHGWGRGPGRPPWWPEDQPFPP
                     RDREAWRGVGRRFLRRVALTIALVFALTVAANGLTYLLLSRILGIEAHRRVLPFGAVL
                     VISLIVAAVASVRAARRIARPMAEVMEAAERVAGGDYGVRVDERGPRDLRRLARSFNA
                     MTERLEAGEARRRDLLADIAHELRTPLSVIQGNAEGMLDGLYPADRAHLEPVIEEARV
                     MSRLLEDLRTLSTAEAGALRLHREPADPKRLVEDAVASFRARADERGVMLEARAANGL
                     TDLDVDPVRIGEVLANLLSNALRHTPRGGRVQVSVEPGTEPGAVAFAVADTGPGIAED
                     LLPHVFDRFVTAAAAGPGAGAAGAGLGLAIARRLVEAHGGTIAAENAPQGGATIRFTL
                     PTASS"
     gene            complement(1237..1920)
                     /locus_tag="VEO00_06050"
     CDS             complement(1237..1920)
                     /locus_tag="VEO00_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU57596.1"
                     /translation="MRTILVVEDEMRIARLVRDFLEHAGFAVIVTGDGEAALASARSV
                     RPDLVVLDLGLPGRDGLDVARELRRTSNVPIVMLTARGDETDRVTGLELGADDYVVKP
                     FSPKELVARVRAVLRRTDAARVGGAEVLRVAGVEVDVPRMRATVEGRPVELTPTEFQL
                     LATLVRQPGRVFTRAQLLDAVHGVAFESYERAIDAHVKNLRRKIEPEPGRPRYLLTVH
                     GVGYRFADA"
     gene            complement(2036..2410)
                     /locus_tag="VEO00_06055"
     CDS             complement(2036..2410)
                     /locus_tag="VEO00_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57597.1"
                     /translation="MRRTWIGVVLLLIAVSIAVGIGAYNAGYSNGLESGAGEVVRVVG
                     PGYGYHGFPFGLLLFPLFLFGIFFLIRGAFWARHGHPHGEHGWGPGWSRRNELFEEWH
                     RHQHEEGDRPAGSGSGGEPANV"
BASE COUNT          323 a          967 c          899 g          304 t
ORIGIN      
        1 tccggtcccg gggggacggg cgctcccgcc tcggtccggc cgggggacga tcaggaggaa
       61 gcggtcggca gggtgaaccg gatcgtcgcg cccccctgcg gggcgttctc ggcggcgatc
      121 gtcccgccgt gcgcctccac caggcggcgg gcgatggcca gcccgagccc cgcgccggcc
      181 gctccggcgc ccggtccggc ggctgcggcg gtgacgaagc gatcgaagac gtgggggagc
      241 aggtcctcgg cgatgcccgg cccggtgtcg gccacggcga acgcgacggc gcccggttcc
      301 gttccgggtt cgacagacac ctgcacgcgg ccgccgcgcg gcgtgtggcg cagcgcgttc
      361 gagagcaggt tcgccaggac ctcgccgatc cgcacaggat cgacgtccag gtcggtaagc
      421 ccgttcgcgg cccgggcctc gagcatgaca ccccgctcgt ccgcgcgcgc ccggaacgag
      481 gcgaccgcgt cctcgaccag ccgcttcggg tcggccggct cgcggtgcag gcgcagggcc
      541 ccggcttcgg ccgtcgacag cgtccgcagg tcctcgagca gccgcgacat cacccgcgcc
      601 tcctcgatga ccggctccag gtgcgcgcgg tcggccgggt agagcccgtc gagcatgccc
      661 tcggcgttgc cctggatcac cgagaggggc gtgcgcagct cgtgggcgat gtcggccagc
      721 aggtcccggc ggcgcgcctc gccggcctcg agccgctcgg tcatcgcgtt gaacgaccgc
      781 gccaggcggc gcaggtcgcg cggcccgcgc tcgtcgaccc gcacgccgta gtcgccgccg
      841 gccacccgtt cggccgcctc catcacctcc gccatcggcc gggcgatgcg tcgcgccgcg
      901 cgcacgctgg cgacggcggc gacgatcagg ctgatcacca ggacggcccc gaacggcagg
      961 acccggcggt gggcctcgat gcccaggatg cgggagagca gcaggtaggt caggccgttc
     1021 gcggcgacgg tcagcgcgaa gaccagcgcg atcgtcagcg cgacgcggcg caggaaccgc
     1081 ctgcccacgc cgcgccaggc ctcgcggtcg cgcggcggaa agggctggtc ctccggccac
     1141 cacggcgggc ggcccggtcc ccggccccag ccgtgctcgt accctggacc gtggccgtgg
     1201 cgccagggtg ggccccaccc gcggccgccg gagtcgctag gcatcggcga agcggtagcc
     1261 gaccccgtgg acggtcagca ggtaccgcgg ccggcccggc tcgggctcga tcttccggcg
     1321 caggttcttc acgtgcgcgt cgatggcgcg ctcgtaggac tcgaacgcca cgccgtgcac
     1381 cgcgtcgagc agctgcgcgc gggtgaacac gcgccccggc tggcggacga gcgtcgccag
     1441 gagctggaac tcggtcgggg tcaactcgac cggtcggccc tccaccgtgg cccgcatccg
     1501 cggcacgtcg acctcgaccc cggccacgcg caggacctcc gcgccgccga cccgggcggc
     1561 gtcggtccgg cgcagcaccg cccgaacccg ggcgacgagc tccttcgggc tgaagggctt
     1621 cacgacgtag tcgtcggcgc ccagctcgag ccccgtcacc cggtcggtct cgtccccgcg
     1681 cgcggtgagc atgacgatcg gcacgttgga ggtccggcgc agctcgcggg cgacgtccag
     1741 gccgtcgcgc cccggcaggc cgaggtcgag cacgaccagg tccggccgga cgctcctcgc
     1801 gctggcgagt gccgcctcac cgtcgccggt gacgatgacc gcgaaccccg cgtgctcgag
     1861 gaagtcacgg accagccggg cgatccgcat ctcgtcctcc accaccagga tcgtccgcac
     1921 cgccggttcc cccctcccag ggtcaagcgt ggcgacgcga cggggcggag gcaagcgccc
     1981 ccgcccgtcc cgccggcgga tccggctcgc ggaccccccc gccatcgccg gttggtcaga
     2041 cgttcgcggg ctcaccgccc gacccggagc ctgccggccg gtcgccttcc tcgtgctggt
     2101 ggcggtgcca ttcctcgaac agctcgttcc ggcgcgacca gcccgggccc cagccgtgct
     2161 caccgtgggg gtgtccgtgg cgggcccaga acgctccgcg gatcaggaag aagatcccga
     2221 acaggaacag cgggaacagc agcagcccga aggggaagcc gtggtatccg tagccggggc
     2281 cgaccacgcg caccacctct ccggcgccgc tctccaggcc gttcgagtac ccggcgttgt
     2341 aggcgccgat cccgacggcg atcgacaccg cgatcagcag gagcaccacg ccgatccagg
     2401 tccgtcgcat ctcgtcctcc tttcgagggt tccatccctc gtttcaccaa cgccatcgtg
     2461 cggccgcgct gtgaagggct cgtgaacgcg gtg
//
LOCUS       DASPWO010000269         4518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_889244, whole genome shotgun sequence.
ACCESSION   DASPWO010000269 DASPWO010000000
VERSION     DASPWO010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889244"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VEO00_06060"
     CDS             <1..367
                     /locus_tag="VEO00_06060"
                     /inference="COORDINATES: protein motif:HMM:NF022219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HYU57598.1"
                     /translation="EARRIRPQSGALDHLQRVHDQISAELRQSLPEDLYRELDDLTPE
                     IRDGTLEELALAHAEILGWLEGLFQGTQLAIRAQAANAAGQLQRQMEEAAHAGLPEGA
                     RPGQRPDGEEPGPSRGTYL"
     gene            complement(384..755)
                     /locus_tag="VEO00_06065"
     CDS             complement(384..755)
                     /locus_tag="VEO00_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57599.1"
                     /translation="MTRRPALAVLLALLGSACGLTGPLEVPSPEPTGPYVITAIDYHF
                     HDAHPSRPVSLSRAVAWNSQARNVHNVTIEGTDFSMDLPPGERIEIDPISSLLPEPGR
                     YRFFCAYHVDRGMRGVLVLVP"
     gene            complement(752..1474)
                     /locus_tag="VEO00_06070"
     CDS             complement(752..1474)
                     /locus_tag="VEO00_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014650980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HYU57600.1"
                     /translation="MIRVEGVHKWFGNNHVLKGIDISVAPGEVLVVIGPSGSGKSTLL
                     RCMNLLEEPTRGRVYFDGHDITDIRTDLNKVRTHIGIVFQAFNLFPHMTARRNITVAL
                     EKVLKLSREEARERALEELRHVGLGERGDAYPGRLSGGQQQRVAIARALAMRPRLMLF
                     DEVTSALDPELVQEVLDVMQRLASEGMTMVVVTHEMGFARGVGTHLVFMDDGEIVEQG
                     EPGAVFDRPERERTQRFLSKVL"
     gene            complement(1504..2550)
                     /locus_tag="VEO00_06075"
     CDS             complement(1504..2550)
                     /locus_tag="VEO00_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HYU57601.1"
                     /translation="MEAAEVRGGREETLGAAILVLGAVAVAVAGLGPIAMVAIRGFTP
                     YGHVVDGLTASSTGALLLVAIGMGAGAIAAGAATFRSMPTKPSRERAISGAILGVHAV
                     TLAALLLWFRSGDPERFAFHFLNFEKLSGYGFAFLRGAKNTVLLAFVGEGGGVILGLV
                     LAILVLSKRAVTRAPARAYINFFRGTPLIWQLSIFYFGFALGLGLHLSAYNSAMIVFA
                     LNTGAYAAEVFRAGIQSIERGQMEAARSLGMSYLQAMRHAIVPQAIRRVIPPLMNEFV
                     ILIKDTSLIVVLGLLDRELDLYSLARQGYSSTFNATFYVAAAAGYLAVTLPLIRAVNA
                     VERRLRSGLVGVGA"
     gene            complement(2621..3490)
                     /locus_tag="VEO00_06080"
     CDS             complement(2621..3490)
                     /locus_tag="VEO00_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HYU57602.1"
                     /translation="MRGRSAILALVAVLAIAAAACGGNGETGGGGTPTASATTGEPLP
                     EFTTLQEGVLTVGSCLDYAPFETVEGGDEVGFDVDLSEEIAKRLGLTVKWVTADFDTI
                     FTAVAADQFDMVAAAVTATGKDGEERDQVVDFSDFYFNSRQSLTVNSEQTPDITSTDD
                     LGEGDIVGAQKGTTGLTWARDNLEPQGVEIKTFQTAPDAFRDLEAGNIQGVINDEQSS
                     VGIIEDLPALEVVEPIDTNEKYAFPFSPSNPELREAVDIVLKQIIDDGTYAEIFEKYF
                     PGQEVPPEYQPAG"
     gene            complement(3566..>4518)
                     /locus_tag="VEO00_06085"
     CDS             complement(3566..>4518)
                     /locus_tag="VEO00_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYU57603.1"
                     /translation="LLERFSIGHDRGSSHGRSRIFRFSYHDPRYVRMAQEALPLWRAL
                     EAEAGEAILQTTGGLDTGTALDDHLAALEACGARFEVLDGVEVRRRFPALSLPDGTAA
                     LFQPDAGIVLADRAWRAFVAGAVAAGAAVREEAPALAIRPGDESVEVDAGGDRIHAGA
                     AVVTAGAWAPGLLGPLGIDLPLRPTRETVAFFRLPEEARIPTVVDWGEAAVYALPSPG
                     QGVKAGEHQAGPTADPDRNGGPDTVSVGRLGAWVAERYPGADPEPHLVQTCLYTNAPG
                     DEFVLERRGPVVVGSPCSGHGFKFAPWVGERLADLAEETL"
BASE COUNT          679 a         1707 c         1508 g          624 t
ORIGIN      
        1 ggaggcacgc cggatccggc cgcagtcggg tgcgctcgac cacctgcagc gagtccacga
       61 ccagatcagc gcggagctgc gccagtcgct ccccgaggac ctgtaccggg agctcgacga
      121 cctgacgccg gagatccgcg atggcaccct cgaggaactg gcgctggccc acgccgagat
      181 cctgggatgg ctcgagggcc tgttccaggg gacgcagctg gccatccggg cccaggcggc
      241 gaacgcggcc gggcagctgc aacggcagat ggaggaagcg gcgcacgccg gcctccccga
      301 gggcgcccgc cccggccagc gccccgacgg cgaggagccc ggcccctctc gtggcacgta
      361 cctgtagcgc cgcagcccgc gtcctacggc accaggacca ggaccccacg catcccgcgg
      421 tctacgtggt aggcgcagaa gaaccggtag cggcccggct ccgggagcag cgacgagatc
      481 gggtcgatct cgatccgctc cccgggcgga aggtccatcg agaagtccgt cccctcgatg
      541 gtgacgttgt gcacgttccg cgcctgactg ttccaggcca cggcccgtga cagcgagacc
      601 ggcctggacg ggtgcgcgtc gtggaagtgg tagtcgatcg cggtgatcac gtaaggcccg
      661 gtcggctccg gcgacgggac ctccagcggt cccgtgagcc cgcaggccga gccgagcagg
      721 gcgaggagca ccgcgagcgc cggccggcgg gtcacagcac tttcgacagg aaccgctggg
      781 tccgctcccg ctccgggcgg tcgaacaccg cgcccggctc gccctgctcg acgatctcgc
      841 cgtcgtccat gaacaccagg tgcgtcccca ccccccgggc gaagcccatc tcgtgggtga
      901 cgaccaccat cgtcatcccc tcggacgcca gtcgctgcat gacgtcgagc acctcctgca
      961 ccagctcggg gtccagggcc gacgtgacct cgtcgaagag catcaggcga gggcgcatgg
     1021 ccagcgcccg ggcgatggcc acgcgctgct gctgcccgcc cgacagccgg cccgggtagg
     1081 cgtcaccgcg ctcccccagc ccgacgtgcc gcagctcctc gagcgcccgc tcccgtgcct
     1141 cctctcggga gagcttcagc accttctcca gggcgaccgt gatgttgcgc cgcgcggtca
     1201 tgtgcgggaa caggttgaac gcctggaaca cgatgccgat gtgggtgcgc accttgttca
     1261 ggtcggtgcg gatgtcggtg atgtcgtgac cgtcgaagta gacccgccct ctcgtcggct
     1321 cctccagcag gttcatgcag cgcagcagcg tggacttccc ggagccggat gggccgatca
     1381 ccacgagcac ctcaccggga gccaccgaga tgtcgatccc cttcaggacg tggttgttgc
     1441 cgaaccactt gtgcacgccc tccacgcgga tcagcgccgc ccgtcggtgc gctgccggct
     1501 ccgtcacgcg cccaccccga ccaggccgct gcgcaggcgg cgctcgaccg cgttgaccgc
     1561 cctgatcagc ggcagcgtca ccgccaggta gccggccgcg gcggccacgt agaacgtcgc
     1621 gttgaacgtc gaggagtacc cctggcgggc gagcgaatac agatccagct cccggtccag
     1681 cagcccgagg accacgatca gcgaggtgtc cttgatcagg atgacgaact cgttcatcag
     1741 cggggggatc acgcgtcgga tcgcctgcgg gacgatcgcg tggcgcatcg cctgcaggta
     1801 ggacatcccc agcgatcgcg ccgcctccat ctgcccccgt tcgatcgatt ggatgccggc
     1861 gcggaacacc tctgcggcgt aggccccggt gttcagcgcg aagacgatca tcgcgctgtt
     1921 gtacgcggac aggtgcaggc cgagaccgag ggcgaagcca aagtagaaga tcgagagctg
     1981 ccagatcagc ggcgtcccgc ggaagaagtt gatgtaggcg cgggcggggg cgcgggtcac
     2041 cgcgcgcttc gagagcacca ggatcgccag gaccaggcca aggatgacac caccgccctc
     2101 cccgacgaac gccaggagga ccgtgttctt cgccccgcgc aggaaggcga acccgtatcc
     2161 cgacagcttc tcgaagttca ggaagtggaa cgcgaaccgc tccgggtcgc cggagcggaa
     2221 ccagaggagc agagctgcca gcgtcacggc gtggacgccg aggatcgctc ccgagatcgc
     2281 ccgttcgcga gacggcttcg tcggcatcga ccggaacgtc gccgcgcccg cggcgatcgc
     2341 cccggcgccc atcccgatcg ccaccagaag cagcgcgccg gtcgacgacg ccgtcaggcc
     2401 atcgacgacg tgcccgtacg gcgtgaagcc ccggatcgcg accatcgcga tcggtccgag
     2461 cccggcgacg gccacggcga ccgccccgag cacgaggatc gcggccccga gagtctcctc
     2521 ccggccgccc cgtacctcag ctgcctccat cgcattccct cccggaaagg gcggggcccg
     2581 cgcccggatc cggacgcggg ccccagccgg tcgctctccg tcagccggcg ggctggtact
     2641 ccggcggcac ttcctggccg gggaagtact tctcgaagat ctcggcgtac gtcccgtcgt
     2701 cgatgatctg cttcagcacg atgtccaccg cctcgcgcag ctccggattg ctgggcgaga
     2761 acgggaaggc gtacttctcg ttcgtgtcga tcggctccac cacctcgagc gccgggagat
     2821 cctcgatgat ccccaccgac gactgctcgt cgttgatcac cccctggatg ttcccggcct
     2881 cgaggtcgcg gaacgcgtcc ggcgccgtct ggaaggtctt gatctccacg ccctgtggct
     2941 ccagattgtc gcgcgcccac gtcaggccgg tggtcccctt ctgcgcgccg acgatgtcgc
     3001 cctcgccgag gtcgtcggtg gacgtgatgt cgggcgtctg ctcggagttc accgtcagcg
     3061 actggcggga gttgaagtag aagtcggaga agtcgacaac ctggtcgcgc tcctcgccgt
     3121 ccttgccggt cgccgtcact gcggccgcca ccatgtcgaa ctggtccgcc gccaccgcgg
     3181 tgaagatcgt gtcgaagtcg gccgtcaccc acttcaccgt caggcccagc cgtttggcga
     3241 tctcctcgct gaggtcgacg tcgaacccca cctcgtctcc gccttcgacc gtctcgaacg
     3301 gtgcgtagtc caggcaggag ccgaccgtca gcacgccctc ctgcagggtc gtgaactcgg
     3361 gtagcggctc gcccgtcgtc gcagatgcgg tgggcgtgcc gccaccgccc gtctcgccgt
     3421 tcccgccgca cgccgccgcc gcgatggcca gtaccgcgac gagcgccagt atcgcgcttc
     3481 gtcccctcat ctgtcccccc tccgttgcat ccggccgatc ccacccgtcg cacacatcct
     3541 gccagcggtg gaagcggcgc tcaacctaca gcgtctcctc ggccaggtcc gccaggcgct
     3601 cgccgaccca cggggcgaac ttgaacccgt gcccgctgca cggtgagccg accaccaccg
     3661 gaccgcgccg ctcgaggacg aactcgtcgc ccggcgcgtt cgtgtacagg caggtctgga
     3721 ccaggtgcgg ctccgggtcg gcccccgggt accgctcggc gacccaggcc cccagccggc
     3781 cgaccgagac ggtgtccggc cccccgttcc ggtcggggtc ggccgtgggc ccggcctggt
     3841 gctccccggc cttcacgccc tgccccgggc tcggcagcgc gtacacggcc gcctcccccc
     3901 agtcgacgac cgtcgggatc cgcgcctcct ccggcaggcg gaagaaagcc accgtctcgc
     3961 gggtcgggcg cagcgggaga tcgatgccga gcgggccgag caatcccggc gcccacgcgc
     4021 ccgcggtgac caccgccgcc ccagcgtgga tcctgtcgcc gccggcgtcc acctccacac
     4081 tctcgtcccc ggggcggatc gccagggccg gagcctcctc gcggaccgcc gccccggcgg
     4141 cgaccgctcc ggcgacgaac gcccgccagg cgcggtcggc caggacgatg ccggcgtcgg
     4201 gctggaacag cgccgcggtc ccgtcgggga gcgagagggc cgggaaccgc cggcgcacct
     4261 ccaccccgtc gagcacctcg aaccgcgccc cgcacgcctc gagcgccgca agatggtcgt
     4321 cgagcgccgt ccccgtgtcc agcccgcccg tggtctgaag gatcgcctct cccgcctcgg
     4381 cctccagggc ccgccacagc gggagcgcct cttgggccat ccgcacgtag cgcgggtcgt
     4441 ggtaggagaa ccggaagatc cgcgaccggc cgtggctcga cccgcggtcg tgcccgatcg
     4501 agaaccgctc caggagga
//
LOCUS       DASPWO010000270         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_297133, whole genome shotgun sequence.
ACCESSION   DASPWO010000270 DASPWO010000000
VERSION     DASPWO010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297133"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1390
                     /locus_tag="VEO00_06090"
     CDS             146..1390
                     /locus_tag="VEO00_06090"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HYU57604.1"
                     /translation="MSSGSAVPPGPASRRLGVGTVLWALIPALSVGFLAAVPFAHAAI
                     RLKQPRMWAVTAAYGAGTLVALVFSTGPEGGQGGEVLGYLVIALMIVATVHAFALRRR
                     VFAPSQQQEVIAAALAARRRRERARAITVRDPALARELRIGRPDLPRQFDDGGLIDVN
                     HVPAQVLVGKLGLSPEQAAQVAETRERLGGLTGPEELVGFSELSEGTVNAVRERLLFL
                     ADGVDVSAPPKPEAQPEPEQPPVDGHDDQPGTAAAPEDHQDDRPGAAAEDGQGLPPPG
                     WYPDPAGGPGVRWWDGTRWADRAAEPPLATAARQTSRQLSFAFIVEPVPVAIGFFFAV
                     FVVAMSSDGCASDSCFDRINLTLVLLLLVVQAAVVVACGAIAVVGSWKRRIAVKRWAV
                     IALPVGSVITWVAALLMIDAAP"
     gene            1578..2054
                     /gene="dut"
                     /locus_tag="VEO00_06095"
     CDS             1578..2054
                     /gene="dut"
                     /locus_tag="VEO00_06095"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HYU57605.1"
                     /translation="MEAVDLPVLRLDPEQPLPAYAHQGDAGADLCAAEDVVLPPGGRA
                     VVGTGLAVAVPDGFAAFVHPRSGLAARHGIALVNAPGTIDAGYRGEVRVILLNTDPAE
                     PFTVRRGDRIAQLVIQPVVRARFVVVDELPPLAPAPAASASPARARGEGGFGSTGR"
     gene            2180..>2486
                     /locus_tag="VEO00_06100"
     CDS             2180..>2486
                     /locus_tag="VEO00_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57606.1"
                     /translation="MDPRSGQSWGEQLADESAAAVGDERSTRKLVTDAMKVVSGRLGD
                     IGRHGIQPLLDEAAAQVRPATLQELRGRFPGLPDDEVAHRLIDRAARTAAGVALGIGG
                     "
BASE COUNT          305 a          886 c          946 g          349 t
ORIGIN      
        1 cggccggacc cgcgcaccgc cgcgaccgtt ctggccagcc gagccaggtg ccccaccggc
       61 ggaccgaccg tggcctcgtc aagcaaggca agggtccgta tactctgttc gcaggagcct
      121 tggagccccg gcgctggagg gacttgtgag ctcgggttcc gccgtcccgc ctggacccgc
      181 gagcaggcga ctgggtgtcg gcaccgtcct gtgggcgctg atccccgcgc tctcggtggg
      241 ctttctggca gccgtcccct tcgcccatgc ggcgatccgg ctcaagcagc cgcggatgtg
      301 ggcggtcacc gccgcctatg gcgccggcac gctggtcgcg ctcgtcttct cgacgggccc
      361 cgagggcggc cagggcggcg aggtgctcgg atacctggtc atcgcgctca tgatcgtcgc
      421 gacggtccat gcgtttgcgc tgcgccgccg cgtgttcgcg ccctcgcagc agcaggaggt
      481 gatcgccgcc gcgcttgccg ccagaaggcg gcgtgagcgg gccagggcga tcacggtccg
      541 cgatcccgcg ctggctcgcg agttgcgcat cggccgcccg gacctgccgc gccagttcga
      601 tgacggcggc ctgatcgacg tcaatcacgt gcccgcgcag gtcctggtcg gaaagctcgg
      661 cctgtcgccg gagcaggcgg cgcaggtggc cgagacgcgt gagcgccttg gcgggctcac
      721 cggcccggag gagctcgtcg gcttcagcga gctgtcggag gggaccgtga acgccgtgcg
      781 ggaacgactg ctgttccttg cggacggcgt ggacgtctcc gccccaccga agccggaggc
      841 gcagccggag ccggagcaac cgccggtcga cggccacgac gaccagcccg ggacggcggc
      901 ggcgcccgag gaccaccagg acgaccgccc cggggcggcg gcagaggacg gccagggcct
      961 gcccccacca ggctggtacc cggatccggc gggtggcccg ggggtgcgct ggtgggatgg
     1021 tacgcgctgg gccgaccggg cggccgagcc ccccctcgcg acagcggcca gacagacctc
     1081 gcgccagctc tctttcgcct tcatcgtcga gcccgtcccg gtcgcgatcg ggttcttctt
     1141 cgcggtgttc gtcgtcgcaa tgtcgtccga tgggtgcgcg tcggacagct gcttcgaccg
     1201 gatcaacctc accttggtgc tgctgctgct ggtcgtccag gcggcggtgg tggtcgcgtg
     1261 cggggcgatc gccgtcgtgg gaagctggaa gcggcggatc gcagtcaagc gctgggcggt
     1321 catcgcgttg ccggtcggca gtgtgatcac ctgggtcgcc gccctgctga tgatcgacgc
     1381 cgcgccatag cccgcgccag cgaggcgtcg ctcgcggata tgcttccatc cggcccgccg
     1441 gccgtgtaaa atcacatcgt tgtaattcgc ccgccggatg gccggacggc cgtcccgcgg
     1501 ggacaggatg ggcctcgggc cgcgagggga agcgtggtcc ggggccctat ccatttgcgt
     1561 gactgaaggg aggcgcggtg gaagctgtcg accttccggt gctccggctg gaccctgagc
     1621 agccgctgcc cgcctatgcg caccagggcg acgccggcgc cgacctgtgc gcggccgagg
     1681 acgtcgtgct gccgcccggc ggcagggcgg tggtcggcac cgggctggcg gtcgcggtgc
     1741 ccgacggctt cgcggccttc gtgcatcccc gctccgggct cgcggcccgg cacgggatcg
     1801 ccctggtcaa cgcgccaggg acgatcgacg cgggctaccg gggcgaggtg cgcgtgatcc
     1861 tgctcaacac cgatccggcc gagccgttca cggtccggcg cggcgaccgc atcgcccagc
     1921 tcgtgatcca gccggtggtc cgcgcccgct tcgtcgtggt cgacgagctg cccccgctcg
     1981 cccccgcgcc ggcggccagc gcctccccgg cccgcgcgcg gggtgagggc ggcttcggtt
     2041 ccaccgggag atagcgaccc cttcggccgc cgccgacgag acgagcccgc cgttgcggcg
     2101 gggaaccgtt aggctggtag gagagcgccg gcctccggcc ggttgcggcg cggctccggc
     2161 gggaaggcaa gaaggcacga tggatccacg cagcgggcaa tcgtggggag agcagctggc
     2221 cgacgagagc gcggccgcgg tcggcgacga gcgctcgacc cgcaagctgg tcacggatgc
     2281 gatgaaggtg gtcagcgggc gcctcggcga catcggccgc cacgggatcc agcccctgct
     2341 cgacgaggcc gccgcgcagg tgcgaccggc gacgctgcag gagctccgcg gccgcttccc
     2401 gggtctgccc gacgacgagg tcgcccatcg cctgatcgac cgcgcggcgc gcaccgcggc
     2461 gggcgtggcg ctggggatcg gcgggt
//
LOCUS       DASPWO010000271         9081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_632781, whole genome shotgun sequence.
ACCESSION   DASPWO010000271 DASPWO010000000
VERSION     DASPWO010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632781"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VEO00_06105"
     CDS             <1..518
                     /locus_tag="VEO00_06105"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYU57607.1"
                     /translation="IRAGSLDAVVCIQVIEHLHCAAGFVRACRRALSPGGILVVATPN
                     RPTFTPGSAVNPFHVHEYDASELQGLLGTAFDRVHLYGLRHGPRLALIDRLLGEPVPR
                     RLIRGGWAGTPSWVRSHLRGIGPRSFRIAPARPTDLDLLAVCGGVRRGARPGERSKRL
                     RPRSEPPKGRT"
     gene            515..775
                     /locus_tag="VEO00_06110"
     CDS             515..775
                     /locus_tag="VEO00_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57608.1"
                     /translation="MKPEGDRDWPDATVHLGQFDRATANDIAGELEAAGIFWWYKEPG
                     FFSQIWEVGVRLFVDRERLSEAKEIVERVRRGGPSPGARSND"
     gene            768..2273
                     /locus_tag="VEO00_06115"
     CDS             768..2273
                     /locus_tag="VEO00_06115"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HYU57609.1"
                     /translation="MTKRGGLFRHAVASGDPLADRVVLWTRVTTGSEEPVELAWTIAR
                     DPDLRDVVASGTAASDPDADQTVHVEASGLEPGTRYHYGFEARGERSPVARTGTLPGP
                     GTEHLRFAMCSCAKYSAGFFNAYARIADRHDVQFLLHLGDYIYEVSNHPPPSQTPPAD
                     IGRSYDPDNECVTLEDYRRRYAHYRLDPDVQRLHLTHPVIATVDDHEFADGVWREGSI
                     EHREDRDGPWSARRAAAFRSRWEWQPYRMPDPADPERVWRTVDLGGLAELFLIDTRSR
                     RDEPIDGAGMRDPARSQLGPDQRAWLLGALSSSTAAWRLLGNSSVMGHIWDPRLTPAA
                     REGLGILKMVNPEMTGPDPDQWDGYPAERDAILRALARGPDTVVLSGDVHVGLALDLV
                     LDPTAGEPARVGGEFVTTSLTSQNVDDKRGWPIHTGSVPMERAFVESLPHVRYTDFDS
                     HGYVMVDVTPERVLGEWWFVDSVLERTGGEARGAAWQIRRGERRLEPAPVE"
     gene            2284..2559
                     /locus_tag="VEO00_06120"
     CDS             2284..2559
                     /locus_tag="VEO00_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57610.1"
                     /translation="MATREELEALSSEELYERAAARAKHHLDVGFFWNLLRQIPAAEA
                     VVGDLDEAQEDTISLWRRLGDYLEADEGELADALRPYYVEYLLEHGR"
     gene            complement(2635..2997)
                     /locus_tag="VEO00_06125"
     CDS             complement(2635..2997)
                     /locus_tag="VEO00_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57611.1"
                     /translation="MTDENSTEELYRHRHDLGEWSSEPEEIEVRSSKTEVVSFRLPSG
                     ELDVLEDAAQRAAESLSEYIRKAVALRLHGLPIGPSVEVTSGAERLTIRSHIVVSSRR
                     DAPDSFVPDLPPLTVANT"
     gene            complement(4086..5624)
                     /locus_tag="VEO00_06130"
     CDS             complement(4086..5624)
                     /locus_tag="VEO00_06130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU57612.1"
                     /translation="MRVLLWHGWLLEATGSNVYTAKAAEAMRRAGHHVAILCQDPHPE
                     RYPFVDAWGVVDGTGVRLAGDTGVPRATGRVTLLRPEIGRLLPVFVLDEYEGFDVRRF
                     VDLDDAALSGYLERNVQALRAAAAWHRPDAAIAGHVVPGGVVAARALGAGAYVLKVHG
                     SDLEYAIRPQARYRPLAAEALAGARAVTGASRDVLERALAFAPEARGRERVVAPGVEA
                     DRFRPRARREALQDAGARLAADPQPRAGRPDALDDVMREALAARDGRALHDLALRYDQ
                     GAPDPGAAARLRALAAFEGPLVGYCGKLIPQKGVEVLLEALVRARPVAAALLVVGYGQ
                     YREWLAGLVDALDGGDADAVAWLREAGPMALDLDPAAVRAAAGFGSRITFTGRLDHRY
                     APEALAAMDVQVTPSLLEEAFGMVAAEGAAAGNLPLVARHSGLAEVGQSLEAAVGRPG
                     LFTYEPGPGAGARIAAGIERLLGLPEAERHDLRATVSDVARREWSWDHTARELLAPFA
                     GATR"
     gene            complement(5703..5912)
                     /locus_tag="VEO00_06135"
     CDS             complement(5703..5912)
                     /locus_tag="VEO00_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57613.1"
                     /translation="MREDTLKLRGARVHALPRANRTYSGGRVCAVDGCDTRLSVYNRG
                     TLCWQHTPLRYDVLRGRKKKRPEAA"
     gene            complement(6070..6642)
                     /locus_tag="VEO00_06140"
     CDS             complement(6070..6642)
                     /locus_tag="VEO00_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU57614.1"
                     /translation="MGEILIRRAGAGDLEAVRQLLELLSEHQKAWRVFEPRSGYREEI
                     MNRYRRALGAAHARHLLAEDDGELVGMAFAEEITPSGFSDERACEVTSVVVRPSHRRA
                     GVGRGLMAGVIAFARERGLARLTLHTFMPNEEARAFFEGLGFWPRYVQLIGDVEDLAA
                     GLGLPAAAGGTSTAAPAETSSAPTERPAAC"
     gene            complement(6635..7282)
                     /locus_tag="VEO00_06145"
     CDS             complement(6635..7282)
                     /locus_tag="VEO00_06145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HYU57615.1"
                     /translation="MGSVAGERPLARPVPADLPERGFLARVRFLRRNRMYTPRYWVLG
                     LRYLWRFKVRNRHVVTRGMVFIDRSAEVSCRRGLGHLEIGRWVWIGAGNAIRCHEGFL
                     RVGDKVVFGGHNTVNAYLDIEIGEDCILADRIYVCDFDHQYADPAVPIRKQGIVKSPV
                     RIAPDCWIGEKATVLRGVTIGRGSVIGAQTVVNRDVPPLSVAVGNPVRIVGRRGG"
     gene            complement(7275..8201)
                     /locus_tag="VEO00_06150"
     CDS             complement(7275..8201)
                     /locus_tag="VEO00_06150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYU57616.1"
                     /translation="MVATSPPDLEGLDPKAANVVYHDAAARSYEAKWAISFDERCIGY
                     VRERAERMLPARRYGKVLEVGAGTGFFILNLWQAGFVEEAHATDIAPGMIEALRENGR
                     QLGCDVRARPADAEGLPYPDGSFDLVVGHAFLHHLPEPRVFLAEAFRVLRPGGAILVA
                     GEPTVVGDRMARIAGRLAGGAFRLAGRVRPGMVREPAEPATDDERVLRDLEFAVDLHT
                     FEPDTMARWANAAGFSAVRVETEELLSALFGWSVRTIEAEARPGLLGRRWAWFAYRNY
                     LRLYRVDRKVLARLVSRRLFYNLLLYGERPGG"
     gene            complement(8205..8984)
                     /locus_tag="VEO00_06155"
     CDS             complement(8205..8984)
                     /locus_tag="VEO00_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYU57617.1"
                     /translation="MGDLVRLEVEDGVGTIRLDRPPMNAIDEALTHDLREAAEEATAR
                     DDVGAVVLWGGEKIFAAGADVKMMVDMAPAQVAGMITGLQDAFSLVEQIPKVTIAALN
                     GYALGGGCELAMCADLRYAAEDAQLGQPEINLGIIPGSGGTQRLPRLVGFSRAKEIIY
                     SGRFVKAEEARAIGLVDEVFPAEEVYAEAVDAARRYANGPRVALRAAKQAINWGTKLP
                     VPAGLVVEREAFIALFGTEDQKEGMRSFVEKGPGKATFSGR"
BASE COUNT         1339 a         3402 c         3103 g         1237 t
ORIGIN      
        1 cgatccgagc cggctcgctg gacgccgtcg tctgcatcca ggtcatcgag cacctgcact
       61 gcgcggcggg gttcgtacgg gcctgtcgcc gggccctctc ccccggtggg atcctcgtcg
      121 tcgccacccc gaaccgtccg acgttcaccc cggggagcgc ggtgaacccg ttccacgtcc
      181 acgagtacga cgcctccgag ctgcagggcc tgctcggcac cgccttcgac cgcgtccacc
      241 tgtacggcct tcgccacggc ccgcgactgg ccctgatcga ccggctgctc ggcgagccgg
      301 tgccccgacg gctgatccgc gggggctggg ccggcacccc atcctgggtc cggtcgcacc
      361 tgcgcggcat cggcccccgc tcgttccgga tcgcccccgc ccggccgacc gacctggacc
      421 tcctcgcggt ctgtggcggg gttaggcggg gagctcgccc cggagagcga tcgaaacgac
      481 tacgtccgcg gagcgaaccc cccaagggga gaacgtgaag cccgagggcg accgcgactg
      541 gccggacgcg accgtgcacc tcgggcagtt cgaccgggcc acggcgaacg acatcgccgg
      601 cgagctggag gccgccggca tcttctggtg gtacaaggag cccgggttct tcagccagat
      661 ctgggaggtc ggcgtgcgcc tgttcgtgga ccgcgagcgt ctgtccgagg cgaaggagat
      721 cgtcgagcgg gtccgccgcg gcggccccag ccccggcgcg cgatcgaatg actaagcgcg
      781 gcggcctctt ccgccacgcg gtcgccagcg gtgacccgct ggccgaccgc gtggtgctct
      841 ggacccgggt gaccaccggc tcggaggagc ccgtggagct ggcctggacc atcgcgcgcg
      901 acccggacct gcgcgacgtg gtcgcgtcgg gcacggcggc cagcgacccc gacgccgacc
      961 agaccgtcca cgtcgaggca agcgggctcg agcccggcac gcgctaccac tacgggttcg
     1021 aggcccgcgg cgagcgctcg ccggtcgcgc ggacgggtac cctgccgggg ccgggaaccg
     1081 agcacctccg gttcgcgatg tgctcgtgcg ctaagtacag cgcgggcttc ttcaacgcgt
     1141 acgcgcggat cgccgaccgg cacgacgtcc agttcctgct gcacctcggg gactacatct
     1201 acgaggtcag caaccacccc ccgccgagcc agacgccgcc ggcggacatc gggcgttcct
     1261 acgaccccga caacgagtgc gtcacgctcg aggactaccg ccggcggtac gcgcactatc
     1321 ggctggaccc cgacgtccag cgcctgcacc tcacccaccc ggtgatcgcc accgtcgacg
     1381 accacgagtt cgccgacggg gtctggcgtg agggatcgat cgagcaccgg gaggaccgcg
     1441 acggtccctg gtccgcgagg cgggccgccg cgttccgctc ccgctgggag tggcagccct
     1501 atcggatgcc cgacccggcc gacccggaac gggtctggcg caccgtggac ctcggtgggc
     1561 tcgccgaact cttcctgatc gacacgcgat cgcggcgcga cgagccgatc gacggcgcgg
     1621 ggatgcgcga cccggcgcgc tcccagctcg ggcccgacca gcgggcctgg ctcctcggcg
     1681 cgctgtcgtc gtcgacggcg gcctggcggc tcctgggcaa cagctccgtg atggggcaca
     1741 tctgggaccc gcggctcacg ccggcggcgc gcgagggact ggggatcctg aagatggtga
     1801 accccgagat gaccggcccc gacccggacc agtgggacgg ataccccgct gagcgggacg
     1861 ccatcctgcg ggccctggcg agggggcccg acaccgtggt cctctccggc gacgtgcacg
     1921 tcgggctggc cctcgacttg gtgctcgacc cgacggccgg ggagccggcg cgcgtcgggg
     1981 gggaattcgt gacgacgagc ctcacgtcgc agaacgtgga cgacaagcgc ggctggccga
     2041 tccacaccgg ctcggtgccg atggagcggg ccttcgtcga atccctcccg cacgtgcgct
     2101 atacggattt cgacagccac ggctacgtga tggtggacgt gacgcccgaa cgcgtgctcg
     2161 gcgagtggtg gttcgtcgac tccgtcctgg agcgcaccgg cggcgaggcg cgcggcgccg
     2221 cgtggcagat ccgccgcggc gagcggcgcc tggagcccgc cccggtcgag tagcatccgc
     2281 gcgatggcga cccgcgagga gctcgaggcg ctctcgtccg aggagcttta cgagcgcgcc
     2341 gcggcgcgcg ccaagcacca cctcgacgtc ggcttcttct ggaacctgct gcggcagatc
     2401 ccggcggccg aggccgtcgt cggggacctg gacgaggctc aggaggacac catcagcctg
     2461 tggaggcgcc tcggcgacta cctggaggcc gacgagggcg agctggcaga cgccctgcgc
     2521 ccctactacg tcgagtacct cctcgagcac ggccggtagc gatcgatagt agccccgacc
     2581 gcttgagaca gagccacccg cgaatcggcc ctgtcttccc tgatgcagtg gtcctcaggt
     2641 attagccacg gtcagcggcg gtagatcggg gacgaacgaa tcaggcgcat cgcgtcggct
     2701 actcacaacg atatgactcc gaatcgtcag tcgctctgcc ccggaagtca cctcaaccga
     2761 tgggccaatc ggcagaccgt gaagcctcaa ggcaactgct ttcctgatgt actcggagag
     2821 actctcggcc gccctttgcg ccgcgtcttc aagtacatca agctccccag agggtagacg
     2881 gaatgaaacg acttcagtct tcgaggagcg gacctcaatt tcctcgggtt cgctactcca
     2941 ctcgccaaga tcgtgtctgt gccggtagag ctcctccgtc gaattttcgt ccgtcatttg
     3001 ctcctcctgt accactcgat tccggatcct ccgcttccca ccccgtcacc gcgcgccaca
     3061 agtcaggccc acctggaacc tgaacgatac acacaacgca gattcgtcca ccatgttcgc
     3121 gaccgatgat ctgatgtgtc gccgcccgat cttgtttgtt ccgacgaaac cgaggcgcct
     3181 ccatcgcaac ctggaggacc cggcggcggg acatgcgatg gtgccttgtc agctctgcca
     3241 gattagcttc atcccactgc cactcctgga tccgcagacg caaccgtcat ctcctgcaga
     3301 cgaatgtata cttcgtcaga ctgagtatac acaacgcgga ggggggagat ggccgacatc
     3361 gaatacttgc tcctcgcgaa tcatgcggag gcagtgaacg ggcttctgta tgtcctggga
     3421 gcaggctgga cagatcagtg gcggggagta atcccagagg acacgcctcc cccagttacc
     3481 catttcggga tcgctgcgag cgttctggtc ccctggacag agacaaatcg gccacaccat
     3541 ctgtcgatca ggatcgaagg agaggatggt gctccagagc tagggcggct tgaggctgat
     3601 ctcgaggttg ggcggccccc tggctccaga cctggaaccg atcaacgggc agtgatggct
     3661 gtgaatgtca accttcagtt tcctcgggcg gggggatacc ggatcgtcgc ggagctaggc
     3721 gacaaggtgc ggaccacctc attccgagtc aacgacgcgc ccctgccgac cctgcgatag
     3781 gtcaggcaaa cccacggggg cgataggcac tccgccagta ccgcccggcg ggcgggaggc
     3841 gcggcggccg cgtaagcacg gggacgagcc ggccctgcgg atccgcgccc ggcaggatcc
     3901 gggccgccac catcacccac ccgcggtcga ggtagccctg caccacgggg cggttctccg
     3961 ggccctcgga cgtccggcgg cgccggcagc gccatcatcc aggcgaaccc gccttcggtg
     4021 ccgggaccgg ccaggatcgt ggcggccgcg agcgacgcca ccaccgagcg gcgcgtgggg
     4081 gcgcgttacc gcgtggcgcc ggcgaacggg gccagcagct cgcgggccgt gtggtcccag
     4141 gaccactcgc ggcgcgcgac gtcgctgacg gtggcgcgca ggtcgtggcg ctccgcctcc
     4201 ggcaggccga ggagccgttc gatccccgcg gcgatcctcg cccccgcacc gggacccggc
     4261 tcgtacgtga acagtcccgg ccggccgacc gcggcctcga gcgactgccc cacctcggcg
     4321 agcccggagt ggcgcgccac gagcgggagg ttccccgccg ccgccccctc tgccgccacc
     4381 atgccgaagg cctcctcgag cagtgagggc gtgacctgca cgtccatcgc cgcgagcgct
     4441 tccggcgcgt accggtggtc caggcgcccg gtgaaggtga tccgcgaccc gaacccggcc
     4501 gccgcgcgaa cggccgccgg atcgaggtcg agcgccatcg ggccggcctc gcgcagccag
     4561 gccaccgcat ccgcgtcccc gccgtcgagc gcatccacga gccccgccag ccattcgcgg
     4621 tactgtccgt acccgacgac cagcagtgca gcggccacgg gcctcgcccg caccagcgcc
     4681 tcgaggagca cctcgacgcc cttttgcggg atgagcttgc cgcagtatcc gaccagcgga
     4741 ccctcgaacg ccgccagcgc ccgcagccgg gccgcggcgc ccgggtcggg agcgccctgg
     4801 tcgtagcgca gggccagatc gtgcagcgcc cggccgtcgc gcgccgcgag cgcctcccgc
     4861 atcacgtcgt ctagcgcgtc aggccgcccg gcgcgcggct gcgggtcggc ggcgagccgc
     4921 gcccccgcgt cctggagggc ctcgcggcgc gcacgggggc ggaagcggtc ggcctccacg
     4981 cccggtgcca ccacgcgctc ccgcccgcgc gcttcgggcg cgaacgccag cgcgcgctcg
     5041 agcacgtcgc gcgaggcacc ggtcacggcc cgcgcgcccg cgagcgcctc ggccgccagc
     5101 ggccggtagc gcgcctgtgg acggatggcg tactcgaggt cgctgccgtg gaccttcagg
     5161 acgtaggcgc cggcgccgag cgcgcgggcc gcgacgacgc cgccggggac cacgtgcccg
     5221 gcgatcgctg cgtcggggcg gtgccaggcc gccgccgccc gcagcgcctg gacgtttcgc
     5281 tcgaggtagc cggacagcgc ggcatcgtcg aggtcgacga agcgacggac gtcgaacccc
     5341 tcgtattcgt cgagtacgaa caccggcagc aggcgcccga tctccggccg cagcagcgtc
     5401 acccggcccg tggcgcgcgg cacgccggtg tcaccggcga ggcggacgcc cgtcccgtcc
     5461 accacccccc aggcatcgac gaaggggtaa cgctcgggat gggggtcctg gcagaggatg
     5521 gccacgtggt ggccggcgcg gcgcatggcc tcggccgctt tcgcggtgta gacgttcgag
     5581 ccggtggcct cgagcagcca accgtgccac aggaggaccc gcaccggcgg gagcatagcg
     5641 gcgggggacg cctcgggccg ggacgcgtgg tcccggcccg cccaactcgt ctgccggttc
     5701 ggctacgccg cctcggggcg cttcttcttg cggccgcgca gcacgtcgta gcgcagcggc
     5761 gtgtgctgcc agcacagggt tccgcggttg tagaccgaca ggcgggtgtc gcaaccgtcg
     5821 acggcgcaga cccgcccacc ggagtacgtg cggttcgccc tcgggagcgc gtggacgcgc
     5881 gctccccgga gcttcagggt gtcctctcgc atccggtgac cttcctccgg tccagtggcc
     5941 gtggccacgg gacccactag acctgcctca cctctgatac gaacggacgg cgccccggga
     6001 tcattcccga ccggagtgat gcgaatcaca ctccccgttc gcgaaggcgt cccgggggaa
     6061 acgtgctggt cagcaggccg ccggacgctc cgtgggcgcc gaggacgtct ccgccggtgc
     6121 ggccgtggac gtcccgccgg ccgccgccgg caggccgagg ccggccgcca ggtcctccac
     6181 gtccccgatc agctggacgt agcgcggcca gaagcccagc ccctcgaaga acgcccgtgc
     6241 ctcctcgttc ggcatgaacg tgtgcagcgt cagtcgggcc aggccgcgct cccgggcgaa
     6301 ggcgatcacc ccggccatca gcccccggcc cacaccggcc cggcggtggg aggggcggac
     6361 gacaacgctc gtcacctcgc aggcccgctc atcggagaac cccgagggcg tgatctcctc
     6421 ggcgaaagcc atccctacca gctcgccgtc gtcctcggcg agcaggtgcc gggcgtgcgc
     6481 ggcccccagg gctctccggt agcgattcat gatctcctcg cggtagccgg accggggctc
     6541 gaagacgcgc cacgccttct gatgctcgga gagcagctcg aggagctggc ggacggcctc
     6601 caggtcgcca gccccggcgc ggcggatcag gatctcaccc accgcgccgc ccgacgatcc
     6661 gcaccgggtt gccgacggcg accgagagcg gcgggacgtc gcggttgacc accgtctgcg
     6721 cgccgatcac cgagccccgc ccgatcgtca ccccgcgcag cacggtggcc ttctcaccga
     6781 tccagcagtc cggcgcgatc cgcaccggcg acttgacgat cccctgcttg cggatcggaa
     6841 ccgccgggtc ggcgtactgg tggtcgaagt cgcagacgta gatgcggtcg gccaggatgc
     6901 agtcctcgcc gatctcgatg tcgaggtacg cgttgacggt gttgtggccg ccgaacacca
     6961 ccttgtcccc cacccgcagg aagccctcgt ggcagcggat ggcgttcccc gcgccgatcc
     7021 acacccagcg gccgatctcc aggtgcccca gcccccggcg gcaggagacc tcggcgctcc
     7081 gatcgatgaa caccatcccg cgggtcacga cgtggcggtt gcggaccttg aagcgccaca
     7141 ggtagcggag cccgagcacc cagtagcgcg gggtgtacat ccggttccgt cgcagaaacc
     7201 ggacccgggc caggaacccg cgctccggga ggtcggccgg caccggccgc gccagcgggc
     7261 gctcgccggc gacgctaccc accaggtcgc tccccgtaga ggagcaggtt gtagaagagc
     7321 cggcgcgaca ccaaccgggc caggaccttc cggtcgaccc ggtacaggcg caggtagttc
     7381 cggtacgcga accatgccca gcgccggccg agcaggcccg gacgagcttc cgcctcgatc
     7441 gtgcgcacgc tccagccgaa cagcgccgag agcagttcct cggtctcgac acgaaccgcg
     7501 ctgaagcccg ccgcgttcgc ccagcgcgcc attgtgtccg gctcgaacgt gtgcaggtcg
     7561 acggcgaact ccaggtcgcg caggacgcgc tcgtcgtcgg tggccggttc ggccggctcc
     7621 cggaccatac cgggccggac ccggccggcc agccggaacg cgccgccggc cagacggccc
     7681 gcgattcgag ccatccggtc ccccaccacc gtcggctccc cggccacgag gatcgcgccg
     7741 cccgggcgca gcacacggaa cgcctcggcc aggaacacgc gcggctccgg caggtggtgc
     7801 aggaacgcgt gcccgaccac caggtcgaat gaaccgtccg ggtacggcag gccctcggcg
     7861 tcggccgggc gggcccgaac gtcgcaccca agctggcggc cgttctcccg cagcgcctcg
     7921 atcatccccg gcgcgatgtc ggtggcgtgc gcctcctcga cgaatccggc ctgccacagg
     7981 ttcaggatga agaacccggt ccccgcgccg acctccaaga ccttcccgta gcgccgcgcc
     8041 gggagcatcc gctcggcccg ctctcggacg tagccgatgc agcgctcgtc gaacgagatc
     8101 gcccacttcg cctcgtagga gcgggccgcc gcgtcgtggt agacgacgtt cgcggccttc
     8161 ggatcgagtc cctccagatc gggcggggac gtggcgacca cgggctaccg cccggagaag
     8221 gttgccttgc ccggaccctt ctcgacgaac gagcgcatgc cctccttctg gtcctcggtg
     8281 ccgaacagcg cgatgaacgc ctcgcgctcg acgacgaggc ccgcgggcac gggcagcttg
     8341 gtcccccagt tgatggcctg cttcgcggcc cgcagcgcga cccgcggccc gttcgcgtag
     8401 cggcgcgccg catcgacggc ctccgcgtag acctcctcgg ccgggaagac ctcgtcgacc
     8461 agcccgatgg cccgcgcctc ctccgccttc acgaaccgcc cggagtagat gatctccttc
     8521 gccctcgaga acccgaccag gcgcggcagg cgctgcgtgc cgccggagcc tgggatgatc
     8581 ccgaggttga tctcgggctg gccgagctgg gcgtcctcgg ccgcgtagcg caggtcggcg
     8641 cacatggcca gctcgcaccc gccacctagc gcgtagccgt tcagcgcggc gatcgtcacc
     8701 ttggggatct gctcgacgag cgagaacgcg tcctgcaggc cggtgatcat ccccgcgacc
     8761 tgcgccgggg ccatgtcgac catcatcttc acgtcggccc ccgccgcgaa gatcttctcg
     8821 ccgccccaca gcacgaccgc cccgacgtcg tcgcgcgcgg tcgcctcctc ggccgcctcg
     8881 cgcaggtcgt gtgtcagcgc ctcgtcgatc gcgttcatcg gcggccggtc gagccggatc
     8941 gtccccaccc cgtcctcgac ctccaaccgc accaaatcgc ccatcgcgtt cccccgttct
     9001 ccgtctcggg ccggtcggcc catggtacgg ggaacccgcg gcggggcgct cgccggtcag
     9061 gcgccggagc gggccaggcc g
//
LOCUS       DASPWO010000272         5951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_297316, whole genome shotgun sequence.
ACCESSION   DASPWO010000272 DASPWO010000000
VERSION     DASPWO010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297316"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..432
                     /locus_tag="VEO00_06160"
     CDS             262..432
                     /locus_tag="VEO00_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HYU57618.1"
                     /translation="MMNALALTGGAATLLWILAVILVIAGIVTLIRGEVLTGIVLIII
                     GALVGPGGVSLF"
     gene            complement(492..881)
                     /locus_tag="VEO00_06165"
     CDS             complement(492..881)
                     /locus_tag="VEO00_06165"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYU57619.1"
                     /translation="MAHPNEELARRGYAAFSSGDMDALNELLADDIKWHVGGRSQLTG
                     EYEGKEAVFGLFARLVELTGGSFKVDLHDVLANDEHTVGLIHATADREGKHLDDNGVQ
                     VFHVRDGKITEAWLHPGDAYASDEFFA"
     gene            complement(959..1750)
                     /locus_tag="VEO00_06170"
     CDS             complement(959..1750)
                     /locus_tag="VEO00_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYU57620.1"
                     /translation="MSDLETLKYGVHNGVATISLNRPTKLNAVNAQMFADLAEASERA
                     SFDPGVRVIVVRAEGRAFSAGIDVNLMGQLAGSRGARFRTWVRGAQQGFLNLARADKP
                     TLAAVHGHALGAGFQIALACDLRIVTHDVSFGMFEVKFGLIPDLGGTHRLTRLVGPAV
                     AKELVWTGRTVDAEEALRLGLANRIVEREVLDKEAEAFARELAASPPIPVSLAKSLIT
                     RATETPLETDLDAAAHAQAQCVETEDHREAVAAFLEKRPPRFTGR"
     gene            1814..2074
                     /locus_tag="VEO00_06175"
     CDS             1814..2074
                     /locus_tag="VEO00_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57621.1"
                     /translation="MAGATCFLCGRPTYDPDKRERPWARAVAGGAVALVCPRCRRDRP
                     EWPALVDHCPACDSTRLSVTLGESVCRECGAVAGLEAPREGL"
     gene            2095..2166
                     /locus_tag="VEO00_06180"
     tRNA            2095..2166
                     /locus_tag="VEO00_06180"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2127..2129,aa:Val,seq:tac)
     gene            complement(2624..4348)
                     /locus_tag="VEO00_06185"
     CDS             complement(2624..4348)
                     /locus_tag="VEO00_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HYU57622.1"
                     /translation="MRRIVQTTLLMAAVAVVLLVPQVTFAATPPVSTFTYTSNMHPLG
                     FSERTNTANPFTANSDLAFWGKTAYHGNYDGFRIIDITEPDNPVELNDYRDCAGNQGD
                     VMVWGHLLIRSWNSPASTTQTCDGQLVGSGFEGLHIFDVSNPADPVLVGSVRLSATLP
                     NKVIVDPPSTAAGTYLASGAAFGPPPPDAGISGDFVLVNDGVNTPTDGCEAFSMPAGA
                     IALVDRGNCTFVQKVHNAQEAGASAVVVVNNVAGLPITMGGTDPTIAIPSVMVSLDDG
                     NLIKSGLPATGTVAHDPTVPPGNCGSHTATLVPDVGHGRVLVYNSASDGACPGLDIIE
                     IPLADPGSASFLHRVPAGRPCHDTGVILGDALKAACAGGNGFSVFSMDPADGGSLATP
                     VLLYSKPVPGVTVGHSATFTWDGNVIVFGHEPGGGGEARCQATSSTVDKSLFFFDADS
                     GAPLGSFVNPRPQTATENCTWHNYNVVPTADRDVLVSAQYQGGISVVDFTDPANAREI
                     AYADPAPLSTTQLILGGDWSDYWYNGRIYESDITRGLLVWRLSDMAVAKAMKLGHLNP
                     QTQEFTIG"
     gene            complement(4632..5138)
                     /locus_tag="VEO00_06190"
     CDS             complement(4632..5138)
                     /locus_tag="VEO00_06190"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYU57623.1"
                     /translation="MEHIRSSIEDAVRYLSEHPDEARYTDSAATAILEEGLRCRVEGP
                     GGASVVTDMPQSVGGGNAAPSPGWLFRAALASCVGTLIAMESAREGLALEGLEVTVDS
                     ESDDRGLLAIDPSVPPGPLSIRVGVRANAAGGEPAALREIIERGKWLCPVCDTATRAV
                     PVTLEIGS"
     gene            complement(5251..5733)
                     /locus_tag="VEO00_06195"
     CDS             complement(5251..5733)
                     /locus_tag="VEO00_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57624.1"
                     /translation="METTLEREVEAPAQRKRSVGWALGSLVALAVAAVAVATVMTVSP
                     RPAVGPAEQARPADAGLYTADEEAVMRLVSQGLIPAKTLEGGVFLIKSLVNRDLIPAE
                     TLKPYQAPVQPLWCPQERALMAAVAAGLVPQEALQGEPFRTKRLIAKGLIPRQSATPC
                     "
BASE COUNT          884 a         2085 c         2054 g          928 t
ORIGIN      
        1 acggtggttc gaggtcgaac gtcaccgcgg tctcgcgaac gtagcgcgcg tggatgtctt
       61 cgatggcggc caggtcgtcc ccagcccccg tccgcaccgt cgtcggcgcc gccgctcgct
      121 ccaccaacag gaggctaggg gccgagtgtt acggacgcgt gacgcgcccc gcgtttcacg
      181 cgtcctcttg ccgggtaccg aagcgactgc cgacggcaag gcagccgctg cccgccggcc
      241 gagtcgaggg agagggacgc gatgatgaac gcacttgccc tgaccggagg agcggccacc
      301 ctgctctgga tcctggccgt catcctggtg atcgccggga tcgtcacgct gatccggggc
      361 gaggtgctga cggggatcgt cctgatcatc atcggggcgc tggtcggccc ggggggcgtg
      421 agcctgttct gaacgcgaag ggggccgcgc ctcttcacgg gcgcggctcc cttgctttcg
      481 gtagccaagt cctacgcgaa gaactcgtcg ctggcgtagg cgtctcccgg atggagccac
      541 gcctccgtga tcttgccgtc gcggacgtgg aacacctgga cgccgttgtc gtccaggtgc
      601 ttgccttccc ggtcggcggt cgcgtggatc agcccgacgg tgtgctcgtc gttcgccagg
      661 acgtcgtgca ggtcgacctt gaagctgccc ccggtcagct ccacgagcct ggcgaagagg
      721 ccgaacacgg cctccttccc ttcgtactca ccggtcagct ggctccgtcc cccgacgtgc
      781 cacttgatgt cgtcggcgag cagctcgttc agcgcgtcca tgtccccgct gctgaacgcc
      841 gcgtaccccc tccgggccag ctcctcgttc ggatgcgcca tgcctgcctc cttcctgttg
      901 gtcggccaac gctacgccaa aggtcggctt ccagcacgag ccgatgaccg gttcgtcgtc
      961 agcgccccgt gaatctcggc gggcgcttct cgaggaaggc ggcgaccgcc tcgcggtggt
     1021 cctcggtctc cacgcactgc gcctgggcgt gcgccgcggc gtcgaggtcc gtttcgagcg
     1081 gggtctctgt ggctcgggtg atcagcgact tcgccaagct gaccgggatc ggtggggacg
     1141 cggccagctc gcgggcgaac gcctcggcct ccttgtcgag cacctcccgc tcgacgatcc
     1201 ggttcgcgag ccccagccgc agcgcctcct cggcgtccac cgtccggccc gtccagacca
     1261 gctccttcgc cacggccgga ccgaccagtc gcgtcagccg gtgcgtgccc ccgaggtcgg
     1321 ggatcagccc gaacttgacc tcgaacatcc cgaagctcac atcgtgggtc acgatgcgaa
     1381 ggtcgcaggc aagcgcgatc tggaagccgg ccccgagcgc gtgcccgtgc actgcggcca
     1441 gggtgggctt gtcggcacgg gcgaggttca ggaacccctg ctgggcgccc cggacccatg
     1501 tccggaaccg cgccccgcgg ctcccggcga gctgccccat caggttgacg tcgatccccg
     1561 ccgagaaggc gcgcccctcg gcacgcacga cgatcacgcg cacgccgggg tcgaaactgg
     1621 cgcgctcgga cgcctcggcc aggtcggcga acatctgggc gttcaccgcg ttcagcttgg
     1681 tcggccggtt cagcgagatc gtggccacgc cgttgtggac gccgtacttc agggtctcga
     1741 gatcggacac gcgcacctcc tcgggaatcc cgcgcgaggt ccccatcctg gcacaccgct
     1801 atccttcgac gcgatggcgg gcgcgacgtg cttcctctgc ggccggccga cctacgaccc
     1861 ggacaagcga gagcggccct gggcccgcgc ggtcgccggc ggcgcggtgg ccctggtctg
     1921 tccgcgctgc cggcgggacc gcccggagtg gccggcgctc gtcgaccact gccccgcctg
     1981 cgacagcacg cggctctccg tgacgctcgg ggagtcggtg tgccgcgaat gcggcgcggt
     2041 cgccggcctt gaggctcccc gggagggcct gtagcctcgg agcgtcggaa acgcgggcgc
     2101 ttagctcagc gggagagcgc cgcccttaca aggcggaggt cggtggttcg aaaccaccag
     2161 cgcccacgac ggaaaaccgc aggtcagggg ccggattcgg tttgcactgc ctcgatggga
     2221 cggaccactc ttgcgccagc gtcacctccc catcacggcg cgcagcaagt cagcgaacgc
     2281 ctgcgggtgc tccacgcggg gaaggagctc cgcacaatga gtggcgtcca ccgatccgcg
     2341 agaatcttgg cggccagggt gagggcgcag aactccccgt aagaatccac cggcgcgatc
     2401 cttctacacc tccggcccgg aggtagtccg gatttcgtac cgccccgggg caccgctcga
     2461 actggcagca ggttgcgggc gaagccggct gagccatcac gggtgagcgc agaagcgaga
     2521 tgcaacccca gcgggaagac atacccgcgc agctcgagcc gggtcgatcg acgcaggggg
     2581 gcgggcccca cgcccgcccc ccgacacgtt cgccgtcaca cggctatccg atcgtgaact
     2641 cctgcgtctg cggattgagg tgcccgagct tcatggcctt cgcgacggcc atgtcgctca
     2701 gcctccagac caggagtccc cgggtgatgt cggattcgta gatgcgcccg ttgtaccagt
     2761 agtccgacca gtcgccgccc aggatgagct gtgtggtcga gaggggggcc gggtccgcgt
     2821 aggcgatctc cctcgcgttg gcggggtcgg tgaagtcgac cacgctgatg cctccctggt
     2881 actgggcgga caccagcacg tcccgatcgg ccgtgggcac gacattgtag ttgtgccacg
     2941 tgcagttctc cgtggccgtc tggggcctcg gattgacgaa gctgcccagc ggagcgcccg
     3001 agtccgcgtc gaagaagaac aacgacttgt cgacggtgct gctcgtcgcc tgacaccgag
     3061 cctcgccgcc gccgccgggc tcgtggccga acacgatgac gttcccgtcc caggtgaacg
     3121 ttgcggagtg accgacggtg acgccgggca ccggcttgga atagagcaag acgggcgtgg
     3181 ccagcgatcc gccgtccgcc gggtccatgc tgaagacgga gaacccgttg cccccggcac
     3241 acgccgcctt cagcgcgtct cccagtatga ccccggtgtc gtggcagggc cggccggcgg
     3301 ggacacggtg caggaagctg gcgctccccg gatcggcgag ggggatctcg atgatgtcga
     3361 ggcccggaca ggccccgtcc gaggcgctgt tgtagacgag cactcgtccg tgcccgacgt
     3421 cgggcacgag ggtggccgtg tgtgacccgc agttcccggg gggcaccgtc gggtcgtgtg
     3481 cgacggtgcc ggtagccggc agcccgctct tgattaggtt gccgtcgtcc agcgacacca
     3541 tgaccgaggg gatggcgatg gtgggatccg tcccgcccat ggtgatcggg agacccgcca
     3601 cgttgttgac cacgaccacc gcgctcgccc cggcctcctg agcgttgtgc accttctgga
     3661 cgaaggtgca gttaccccgg tcgacgagcg cgatggcgcc ggcaggcatg gagaacgcct
     3721 cacagccatc cgtgggggtg ttgacgccgt cgttcaccag gacgaagtcg ccggagatcc
     3781 ccgcgtcggg aggtggggga ccgaacgccg cgccgctggc caggtacgta cccgccgccg
     3841 tcgagggcgg atcgacgatc accttgttcg gcagtgtggc agacagccgc acggagccca
     3901 ccaggacggg atcggccggg ttgctgacgt cgaagatgtg aagcccctcg aatcccgatc
     3961 ccaccagctg gccgtcgcac gtctgggtgg tggaggcggg tgagttccag gaccggatca
     4021 ggaggtgacc ccagaccatg acgtcgccct ggttcccggc gcagtcacgg tagtcgttca
     4081 actccaccgg gttgtcgggt tcggtgatgt cgatgatgcg gaacccgtcg tagttaccgt
     4141 gataggccgt cttcccccag aacgcgaggt cggagttcgc ggtgaaggga ttcgcggtgt
     4201 tcgtgcgttc ggagaatcca agagggtgca tgttgctcgt gtacgtgaac gtgctcaccg
     4261 gcggagtggc ggcaaatgtc acctgaggga ccaggagcac taccgccacc gcggccatga
     4321 gcagggtcgt ctggacgatc cgtcgcattc attcctccca gagggtcgag agcggatcgg
     4381 agtgagtccc tcgacccgcc cggagggccc cggggcgccc agggcgggat ggaatcagtc
     4441 gctgcgggcc tctggagcgc ggaccaacag gcggtgggtg gcgttcgctg cacggatctc
     4501 cgctccgagg aggaatgcga actgtagcca tccgccccat agcggcgcaa acaccagaat
     4561 tcaaccctga cccctccatc atcgacatcc gcgacgtcgc tcccggacga gcgtccagtc
     4621 gccggtcgag ctcaggatcc gatctccagg gtgaccggca ccgcccgggt ggcggtgtcg
     4681 cagactgggc agagccactt ccctcgctcg atgatctcgc ggagtgcggc cggctcgcca
     4741 cccgcggcgt tcgcccgcac gccgacgcgg atcgagagcg gccccggcgg gaccgacggg
     4801 tcgatcgcca ggaggccacg gtcgtccgat tcgctgtcca cggtcacctc cagcccctcg
     4861 agggcgaggc cctcccgcgc ggactccatg gcgatgagcg tgcccacgca gctggcgagc
     4921 gccgcccgga acagccagcc gggcgagggc gccgcattgc cgccgcccac cgactgtggc
     4981 atgtcggtga cgaccgaagc accccccggc ccctccaccc ggcagcgcaa tccctcctcc
     5041 aggatcgccg tggccgccga gtccgtgtag cgagcctcgt ccgggtgctc gctcaggtag
     5101 cggaccgcgt cctcgatcga cgatcggatg tgctccacga tgtctccctt cgcaagcgcc
     5161 gcacgtctca ctcgaggcga gctctgacac tacgacgagg atcgccgagg gtgccggggg
     5221 ttcccggcac cctcgggatg agcgttccgc tcagcagggt gtggcggact gccgggggat
     5281 gagccccttc gcgatcaggc gcttcgtgcg gaacggctcg ccctgcagcg cctcctgcgg
     5341 gaccagcccg gccgcgacgg ccgccatcag ggcacgctcc tgcgggcacc agagtggctg
     5401 gaccggagcc tggtagggct tcagcgtctc ggcggggatc aggtcccggt tcaccaggct
     5461 cttgatcagg aagacgccgc cctccagcgt cttcgccggg atgagcccct gcgacaccag
     5521 ccgcatgacg gcctcttcgt ccgccgtgta caggcccgca tccgcggggc gggcctgctc
     5581 ggccggcccg accgccggcc gcggcgagac cgtcatcacc gtggccacgg caaccgccgc
     5641 caccgccagc gcgacgaggc tgccgagcgc ccacccgacg gaccgcttcc gctgggccgg
     5701 tgcctcgacc tctcgttcga gggtcgtctc catggtgtcc tcccttcctc cgccctcacg
     5761 ccgagggctc gtgaacggag ggtgccggac gccgctggaa gaccccgggg agaacgctgg
     5821 aagaacgctg gaagcgggca aacgcagtat tccgttcgaa cgatcgccgc gctacggttc
     5881 gggaatgcgc ttcctcctgc tcggtccgct cgaggtctcg gacggcgagc ggacactcgc
     5941 actcggcggg c
//
LOCUS       DASPWO010000273         9037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_552052, whole genome shotgun sequence.
ACCESSION   DASPWO010000273 DASPWO010000000
VERSION     DASPWO010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552052"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VEO00_06200"
     CDS             complement(<1..339)
                     /locus_tag="VEO00_06200"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HYU57625.1"
                     /translation="MRIGILTGGGDVPGLNACIKAVVGRVSEQGQEVVGIRRGWNGLY
                     TTDPDDPRTTEANLTPLDPAAVRTIDRSGGTVLHTSRTNPGRVKRSEVPPFLTAEGVE
                     PFDFTPHVLRV"
     gene            528..2036
                     /locus_tag="VEO00_06205"
     CDS             528..2036
                     /locus_tag="VEO00_06205"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYU57626.1"
                     /translation="MTDAYRLVIGGERVDAASGETFDSIDPSTGEAFAAVAKAGQEDV
                     RRAVQAARTAFDEGPWPRLRGRERATAMLKVADLVKARGKELAELEARDSGGTIRKAQ
                     NADVGMVISTFRTYAEMAAREDDEEPLSHGPASLNYLRREPIGVCAAISPWNFPIQMA
                     AWKIAPAIAAGNAVVLKPASFTPLTSLELGAICAEAGVPEGVVSVLSGPGAAAGEALV
                     ESPQVDKVAFTGSTEVGRRIMQLASGSIKKCTLELGGKSPAIVLDDAPLDYAVDGVLW
                     GVFFHQGQVCSAGTRAFVHHAILDDFVAELVKRAEAIRVGPALDPASDIGPVVAKSQL
                     ETVERYVETGREEGAELLTGGERAAVEGHDGGFYYRPTIFAGTNDLSIAQEEIFGPVL
                     VVIPVEDDDEAVRLANRSIYGLAGAVWSGSNPRAIEVAEGLRTGTVWINDYHMINPRF
                     PFGGYKESGLGREHGRLGFDEYRETKHVHVDLLGPDRTRHPWWDITVPKRGG"
     gene            2036..2566
                     /locus_tag="VEO00_06210"
     CDS             2036..2566
                     /locus_tag="VEO00_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HYU57627.1"
                     /translation="MGQVSREEAVATLAAGRERLLALLGRLGEDERERRGTIGGGDWS
                     AKDLLGHVAYWQELALDSLAAWRRGEVPWAEAEVFRGPDGAEHAPNAADVERKRALPY
                     EEVLEVHERTFRALIVEFHGFDDETWWSKAPYPAERRNRLGTMLGAVLGSDRGPFSHA
                     EIHDDDLAAYVGSLNT"
     gene            complement(2577..3026)
                     /locus_tag="VEO00_06215"
     CDS             complement(2577..3026)
                     /locus_tag="VEO00_06215"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYU57628.1"
                     /translation="MRVAVSGVLPAPPARAWTVLADWERQADWMPDADSVTVLGDLRE
                     GVGTRIAVRTRLFGVPAFTEILEVTNWHPGVGLEVRHGGPVRGFGRWRLRPAGDGAEF
                     LWEEEFRLAFGGPLGELAAVVYAPLLRWLIRRAINGLTGRLRTGPPG"
     gene            3074..3538
                     /locus_tag="VEO00_06220"
     CDS             3074..3538
                     /locus_tag="VEO00_06220"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HYU57629.1"
                     /translation="MVFDLVLFSHVALSIVAFGPTFVFPIWLALARRAGPSAPPFAVH
                     AVITIAERLLVPLAILVPATGIALIAETRTDWLHTGWLLASMALYALAFGVATALGLP
                     NARRILALSEGGPDPEAQAEVRRRALWQAVYGYAINLLVLAVLALMVFKPGA"
     gene            3605..3853
                     /locus_tag="VEO00_06225"
     CDS             3605..3853
                     /locus_tag="VEO00_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57630.1"
                     /translation="MPFSWAAPWLILHIASALYGVVLLVAVAVITPSSGKLLALLKDR
                     EAGTPPADPGQFIARGTRLRLGGTFLTISVLVLIALML"
     gene            complement(3972..4391)
                     /locus_tag="VEO00_06230"
     CDS             complement(3972..4391)
                     /locus_tag="VEO00_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYU57631.1"
                     /translation="MDENAIDEVVGRLFHGEPTFDWVGVYVVEGDTLVLGPFRGEPPT
                     GHERIPIPEGVCGAVVGSGETEVVPDVRARPGHIACSLATRSEVVAPVTVDRRVVAVL
                     DVDSDTLGAFHDREVALVETAAAEIAARSFASARPSA"
     gene            complement(4433..>9037)
                     /locus_tag="VEO00_06235"
     CDS             complement(4433..>9037)
                     /locus_tag="VEO00_06235"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0034081 - polyketide synthase complex
                     [Evidence IEA];
                     GO_process: GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HYU57632.1"
                     /translation="LSATGTRPYDEGADGFVMGEGAAVFLLKRLADAERDGDRIHAVI
                     RGLAGSSDGKGRGLTAPNPVGQRLAVEWAWERAALSPATAGLIEGHGTSTALGDVVEV
                     ESLHQAFGPLHLPVGSIALGSAKSNIGHLKAAAGAAGLLKAVFALHEKVLPPSLNFDR
                     PNPNIDWAHSPFSVNTELREWEVPAGGTRRAGVSAFGFGGTNFHAILDEYVPGASNGR
                     GRKSFAVTQGEGPAPGRAAEGPAVPRSPLRGALVVGAGSAAELMTRLRSIETEAGAGR
                     APVVGPPPEAELRAPERVAIDFGDGAELHEKAGLALHALAAENPAEWRALRGRGIFRG
                     SGPAHKVAFLYTGQGSQYVNMLADLRASEPVVRDVFDEADRVMTPLLGKPLSRYVFVG
                     GTDPRALEEAAADLMQTDVTQPAVLAADLALDRLLAEFGISPDMVMGHSLGEYAALVA
                     AGCLTFESAARAVGARGRAVASLAVEDRGRMAAVFAPLPEVDRILRGIDGYVVVANLN
                     SNSQAVISGASDAVERAIRAFDAAGHRVVPLPISHAFHTRIVAPATEPLRRALAGLGL
                     RPARLPIVANVTGEFYPAGSTAVSGMLDMLAAQIASPVQFVKGLQTLYEAGARVFVEL
                     GPKSALHRFVEDVLGDRIDVLPLFTNHPKRGDVVSFNHALCGLYAAGLGAGLPATEAP
                     TTVPVPARALAGLEPADAVPRRVPVPVLRPRLEVCKPTGVPLGPGSRVVIMPDGGGVA
                     KEVIGQLEERGVAPLVVEDDPDAATLQRRLESWAAEGAVQGVYWLPALDGEPSIGAMG
                     PDQWREGLRRRVKLLSTTMRTLYDQIAATGTFLVTATRLGGMHGYDEAGAVAPMGGAV
                     TGFAKAYKRERVHPLVKAVDVEATADPALVAGLLVDETIRDPGTVEIGHSDGLRWSVA
                     LRESPAEDGRPGLPLTSETVFVVTGAAGGIVSAIVADLAAASGGTFHLLDLAPEPDPS
                     DPDLARFVQDRDGLKRVLFDRIKARGGRATPVMVERELASIERKQAGLSAIEAIRSAG
                     GTAHYHELDLTDGAAASAVIDGVRRQHGHIDVLIHAAGIEISHLLPDKEAREFDLVFD
                     VKADGWFNLLHAIGDMPLGATVAFTSVAGRFGNTGQTDYAAANDLLCKCASSLRTTRP
                     GTRAIAIDWTAWADIGMATRGSIPKVMEAAGIDMMLPEIGIPAVRRELVSGGMRGEVV
                     LAGSLGTLTEDWDATGGLDPELATRALGAAGAAGPMVGRVTGMSVHEGLTVETVLDPE
                     AQPFLWDHRMDGTALLPGVMGAEAFAELARLALPGWRVAAVEDMRFQAPFKFYRGEPR
                     TLLLGAVFRPDGQDIVAECRLTGERTLPLRPEPQVTTHFTGKVRLTRAPLEPGRIDAV
                     ALPDGPFIGAADIYSVYFHGPSYRVLERAWPPVVGGTALGVLTEDLPPDRTPASAPLA
                     ASPRLIELCFQTIGIWELATRGRFALPRSFERVTAFGGPEARRVPLVAVVTARDGGER
                     FDAMVADAEGNVCVKLEGYRSVELPGAIAPTELRIFQAAG"
BASE COUNT         1262 a         3275 c         3242 g         1258 t
ORIGIN      
        1 cacccgcagg acgtgagggg tgaagtcgaa gggctccacg ccctcagcgg tgaggaacgg
       61 cggaacctcg ctccgcttca cccggccggg gttcgtgcga gaggtgtgca gcaccgtgcc
      121 gccgctgcgg tcgatcgtcc gcaccgcggc ggggtcgagc ggggtgaggt tcgcctcggt
      181 ggtccgcggg tcgtcggggt cggtggtgta caggccgttc cagccgcggc ggatgccgac
      241 gacctcctgc ccctgttccg agacgcgccc gaccaccgcc ttgatacagg cgttcagccc
      301 ggggacgtca cccccacccg tcaggatgcc gatgcgcact ggttccctcc aaccgctgcg
      361 acccgagtcc cgacatccta ccggccgccg ccggaggctc cgggggttcg cgggtagctc
      421 gcccggtggt gcgatggaca ctgccgcgtc cggcgagcga accccccgag gaggaggagg
      481 agtactcggc gcgggtacca tgccatctgc gaacgaggag gttcgacatg accgacgcgt
      541 accggctggt gatcggcggc gagcgggtgg acgccgcgtc cggcgagacc ttcgactcga
      601 tcgacccctc gaccggcgag gcgttcgccg cggtggcgaa ggccggccag gaggacgtcc
      661 gccgtgcggt gcaggccgcc cggaccgcct tcgacgaggg cccgtggccg cggctgcggg
      721 gacgcgagcg cgccaccgcg atgctgaagg tcgccgacct ggtgaaggcg cgcgggaagg
      781 agctcgccga gctggaggct cgcgactcgg gcgggacgat ccggaaggca cagaacgccg
      841 acgtcgggat ggtgatctcg acgttccgca cctacgcgga gatggcggcg cgcgaggacg
      901 acgaggagcc gctgtcgcac ggcccggcct cgctgaacta cctgcgccgc gagccgatcg
      961 gagtctgcgc ggcgatctcg ccgtggaact tcccgatcca gatggccgcc tggaagatcg
     1021 ccccggccat cgccgcgggg aacgccgtcg tgctgaagcc agcgtccttc acaccgctca
     1081 cctcgctgga gctcggcgcg atctgcgcgg aggccggtgt ccccgagggc gtggtgtccg
     1141 tgctctccgg gccgggcgcc gccgctggcg aggcgctcgt cgagagcccg caggtggaca
     1201 aggtcgcgtt caccgggtcc accgaggtcg ggcgacgaat catgcagctc gcctcggggt
     1261 cgatcaagaa gtgcacgctc gagctcggcg gcaagtcgcc ggcgatcgtc ctggacgacg
     1321 cgccgctcga ctatgcggtg gacggcgtgc tgtggggcgt gttcttccac cagggccagg
     1381 tgtgcagcgc ggggacgcgc gcgttcgtgc accacgcgat cctcgacgac ttcgtggccg
     1441 agctggtcaa gcgcgccgag gcgatccgtg tcgggccggc actcgatccc gcgtccgaca
     1501 tcgggccggt cgtggcgaag agccagctcg agaccgtcga gcgctacgtc gagaccggac
     1561 gggaggaagg ggctgagctc ctgaccggcg gcgagcgcgc ggcggtcgag ggccacgacg
     1621 gcggcttcta ttaccggccg acgatcttcg ccggtacgaa cgacctgtcc atcgcgcagg
     1681 aggagatctt cgggccggtg ctcgtcgtga tcccggtcga ggacgacgac gaggccgtgc
     1741 gcctggcgaa ccgctcgatc tacgggctgg ccggagcggt ctggtcgggg tcgaacccca
     1801 gggcgatcga ggtggcggag gggctgcgca ccgggacggt gtggatcaac gactaccaca
     1861 tgatcaaccc gcgcttcccg ttcggcggct acaaggagtc gggcctcggg cgcgagcacg
     1921 gccgccttgg gttcgacgag taccgcgaga cgaagcacgt ccacgttgac ctgctcggac
     1981 ccgaccgtac ccggcacccc tggtgggaca tcacggtccc gaagcgcggc ggctgatggg
     2041 gcaggtctcg cgcgaggagg cggtcgcgac gctcgcggcc gggcgggagc ggctgctcgc
     2101 gctgctcggc cggctcgggg aggacgagcg cgagcggcga gggacgatcg gcggcgggga
     2161 ctggtcggcg aaggacctcc tcggccacgt cgcctactgg caggagctgg cgctcgactc
     2221 gctggcggcc tggcggcgcg gcgaggtgcc gtgggccgag gcggaggtgt tccgcgggcc
     2281 cgacggcgcc gagcacgccc cgaacgcggc cgacgtcgag cgcaagcggg cgctgccgta
     2341 cgaggaggtg ctcgaggtcc acgagcggac cttccgtgcc ctgatcgtgg aattccacgg
     2401 gttcgacgac gagacctggt ggtcgaaggc cccgtatccg gccgagcgtc gcaaccggct
     2461 cgggacgatg ctcggcgccg tactcggctc ggaccgcggt ccgttctcgc acgcggagat
     2521 ccacgacgac gacctggccg cctacgtcgg atccctgaac acgtaaaggg actgtactat
     2581 ccgggtggac ccgtgcgaag acggccggtg aggccgttga tcgcccggcg gatgagccag
     2641 cgcagcaacg gcgcgtagac gaccgcggcc agttccccga gcggcccgcc gaacgccagc
     2701 cggaactcct cctcccacag gaactccgcg ccatcgccgg ccgggcgaag ccgccatcgc
     2761 ccgaacccgc ggaccggccc gccgtggcgg acctccaggc cgacgcccgg gtgccagttc
     2821 gtaacctcga ggatctcggt gaacgccggc acgccgaaca gacgggtgcg cacggcgatc
     2881 cgggtgccga ccccctcacg caggtcgccg agcaccgtga ccgagtccgc gtcgggcatc
     2941 cagtctgcct ggcgctccca atccgcgagg accgtccagg cccgtgccgg tggcgccggc
     3001 aggaccccgc tcaccgcgac ccgcacgtca ccctctccac cgtctccgcg ggcggtgtga
     3061 tcgaatcggc gccgtggtct tcgacctcgt gctcttctct cacgtcgccc tgtccatcgt
     3121 ggcgttcggc ccgacgttcg tcttcccgat ctggctggcg ctggcgcggc gggccgggcc
     3181 gtccgcgccg ccgttcgcgg tccacgcggt gatcacgatc gccgagcggc tgctggtccc
     3241 cctggcgatc ctcgtcccgg ccaccggcat cgcgctgatc gccgagacga ggaccgactg
     3301 gctgcacacc ggctggctgc tggcgtccat ggcgctgtac gccctggcgt tcggcgtcgc
     3361 caccgccctg gggctgccga acgcccgtcg gatcctggcg ctctccgagg gcggcccgga
     3421 tcccgaggcc caggcggagg tccggcggcg ggccctctgg caggcggtgt acgggtacgc
     3481 gatcaacctc ctggtgctcg cggtgctggc gctgatggtg ttcaaaccgg gcgcgtaggc
     3541 acggaaggtt gaccgtcggc ggcgcttgcc gctttgctgc gccgcggcgc tcacccgaag
     3601 gaggatgccg ttctcgtggg ccgccccgtg gctgatcctg cacatcgcct cggccctgta
     3661 cggcgtcgtg ctcctggtgg ccgtcgccgt catcacgcct tcctccggca agctgctcgc
     3721 gttgctgaaa gacagggagg cggggacccc gccggccgat ccgggccagt tcattgcccg
     3781 cggaacgcga ctccggctcg ggggaacgtt cctcacgatc tcggtcctgg tcctcatcgc
     3841 gctgatgctg tgaaggccgg gcaccgccta cctcccaccg ccggttccgt aggcggtcgc
     3901 gttacggggg ttggcgggta gctcgtcccg tggatcgatc gacccgagca cgacgggcga
     3961 gtggaccccc ctcaggccga cgggcgggcg gacgcgaacg agcgggcggc gatctcggcc
     4021 gccgcggtct cgacgagggc gacctcgcgg tcgtggaacg cgccgagggt gtcggagtcg
     4081 acgtccagga cggcgaccac gcgccggtcg accgtcaccg gggcgaccac ctcggaccgc
     4141 gtcgccaggc tgcaggcgat gtggccgggg cgcgcgcgca cgtcgggcac cacctcggtc
     4201 tcgccgctcc cgacgaccgc gccgcagacg ccctcgggga tcgggatgcg ctcgtggccg
     4261 gtggggggct cgccgcggaa ggggccgagg acgagcgtgt cgccctcgac gacatacacc
     4321 cccacccagt cgaacgtcgg ctcgccgtgg aacaggcgcc cgaccacctc gtcgatcgcg
     4381 ttctcgtcca tggagccaag tgtatcggcg agagaagaag cgctcccctg ctctatcccg
     4441 ccgcctggaa gatcctcagc tcggtcgggg cgatggcgcc cggcagctcg accgaccggt
     4501 agccctccag cttcacgcag acattgccct cggcgtccgc caccatggcg tcgaatctct
     4561 caccaccgtc acgggcggta accaccgcca cgagcgggac tcgccgagct tccgggccac
     4621 cgaacgcggt gacccgttcg aaggaacgcg gcagggcgaa cctgcccctc gtcgcgagct
     4681 cccagatgcc gatggtctgg aagcagagct cgatcagtct cggtgaggcc gcgagcggtg
     4741 cgctcgccgg cgtgcggtcg gggggcaggt cctcggtgag gactcccagg gccgtgcccc
     4801 ccacgaccgg cggccacgct cgctcgagca cccggtacga cggcccatgg aagtagacgc
     4861 tgtagatgtc cgcggccccg atgaaaggac catcggggag tgccaccgca tcgatccggc
     4921 ccggctccag gggtgcgcgc gtcagccgca ccttccccgt gaagtgcgtg gtcacctggg
     4981 gctccgggcg gaggggcagc gttcgttccc ccgtgagccg gcactcggcg acgatgtcct
     5041 ggccgtcggg ccggaagacg gcccccagga ggagcgtccg cggttcgccc cggtagaact
     5101 tgaaaggcgc ctggaaccgc atgtcctcga cggcggcgac ccgccagccc gggagggcga
     5161 gccgagccag ttcggcgaac gcttcagcgc ccatcacgcc gggaagcagc gcggtcccgt
     5221 ccatccggtg gtcccacagg aacggttggg cctccgggtc gaggacggtc tccacggtca
     5281 gaccctcgtg cacgctcatg cccgtgacgc ggccgaccat cgggcccgcg gcgccggccg
     5341 ccccaagggc gcgagtggcg agctcggggt cgagcccgcc cgtggcgtcc cagtcctccg
     5401 tgagggtccc gagactgccg gcgagcacga cctctccgcg catgcctccg gaaaccagct
     5461 ccctgcggac ggcggggatg ccgatctccg gcagcatcat gtcgatgccg gccgcctcca
     5521 tcaccttcgg gatcgaaccc cgggtggcca tcccgatgtc cgcccaggcc gtccagtcga
     5581 tagcgatggc acgcgtcccc ggtcgagtgg tccggaggct ggaagcgcac ttgcacagca
     5641 ggtcgttcgc ggccgcgtag tccgtctgtc ccgtgttgcc gaaccgcccg gccacggagg
     5701 tgaacgcgac cgttgccccg aggggcatgt ccccgatggc gtgcaggagg ttgaaccacc
     5761 cgtcggcctt cacgtcgaag accaggtcga actcccgcgc ttccttgtcc ggaagcaggt
     5821 ggctgatctc gatgcccgcc gcatggatga gcacgtcgat gtggccgtgc tgccggcgga
     5881 ccccatcgat cacggcgctg gctgccgcgc cgtcggtcag gtcgagctcg tggtagtgag
     5941 ccgttccccc cgcggaccgg atggcctcga tcgccgacag gccggcctgc ttccgttcga
     6001 tggatgccag ttcccgctcg accatcaccg gagtggcccg gcctccgcga gccttgatcc
     6061 gatcgaacag gacgcgcttc aggccatccc ggtcctgcac gaaccgggcc aggtcggggt
     6121 cgctcgggtc gggctcgggt gcgagatcca gcaggtggaa cgtccccccc gatgcggcgg
     6181 cgaggtccgc cacaatggcc gagacgatcc ctcccgctgc cccggtcacg acgaagaccg
     6241 tctcggacgt gagcggaagg ccgggccggc cgtcctctgc cggactctcc cgcagggcga
     6301 ccgaccaccg cagcccatcg ctgtgcccga tctcgacggt gccgggatcg cgaatcgtct
     6361 cgtcgacgag cagtccggcg acgagggccg gatcggcggt ggcctccacg tccaccgcct
     6421 tgaccagcgg gtgaacccgt tcccgcttgt aggccttggc gaatcccgtg accgcgcctc
     6481 ccatgggagc cacggcgccc gcttcgtcgt acccgtgcat gccgcccagc ctggtcgcgg
     6541 tgacgaggaa ggtccccgtg gccgcgatct ggtcgtacaa cgtccgcatg gtcgtggaaa
     6601 ggagcttcac ccggcggcga aggccctccc gccactgatc gggccccatc gcgccgatcg
     6661 agggctcccc gtccagggcg ggcagccaat agacgccctg gacggcgccc tcggccgccc
     6721 aggactcgag ccgccgctgc agggtcgcgg catctggatc gtcctccacg accagcgggg
     6781 cgactccgcg ctcctccagc tggccgatca cctccttcgc gacgccgccc ccgtcgggca
     6841 tgatgaccac cctgctgccg gggcccaagg gcacgccggt gggcttgcag acttcgaggc
     6901 ggggccgcag gaccgggacg ggaacgcggc gcggaacggc atcggcgggc tccaggccgg
     6961 ccagtgcccg tgccgggacc gggacggtgg tgggcgcctc cgtggccggg aggccggccc
     7021 ccagtcctgc ggcgtacaag ccgcacaggg cgtggttgaa cgagaccacg tcgccccgct
     7081 tcgggtggtt cgtgaacaat gggaggacgt cgattcggtc gccgagcacg tcctcgacga
     7141 aacgatgaag ggccgacttc ggccccagct cgacgaagac tcgcgcgccg gcctcgtaca
     7201 gcgtctgaag gcccttcacg aactgcaccg gcgaggcgat ctgggcggcc agcatgtcga
     7261 gcattccgga gaccgcggtc gaccccgcgg ggtagaactc gccggtcacg ttagcgacga
     7321 tgggcagcct cgcgggccgc aggccgagtc cggccagcgc ccgccggagc ggctcggtcg
     7381 ccggtgccac gatccgggtg tggaaggcgt ggctgatcgg gagcggcacg acgcggtgcc
     7441 cggcggcgtc gaaggcgcga atggcccgct ccacggcatc gctcgccccg ctgatcaccg
     7501 cctggctgtt gctgttgaga ttggcgacga ccacgtatcc gtcgatgccg cgaaggatcc
     7561 ggtcgacctc cggaaggggc gcgaacacgg cggccatccg cccccggtcc tcgaccgcca
     7621 ggctcgccac cgcccggcct cgcgcgccga ccgcccgcgc ggcgctctcg aacgtgaggc
     7681 atccggccgc cacgagcgcg gcgtactcgc ccaggctgtg ccccatgacc atgtcgggtg
     7741 agatgccgaa ctccgcgagc agcctgtcca gcgcaaggtc ggcggcaagg accgccggct
     7801 gggtcacgtc cgtctgcatc aggtcggccg cagcctcttc cagggccctc gggtcggtcc
     7861 ctccgacgaa gacgtaccga ctgaggggct tccccagcag gggcgtcatc acccggtcgg
     7921 cttcgtcgaa cacgtcccgc acgacgggct cggaggcgcg gaggtccgcc agcatgttca
     7981 cgtactgcga gccctggccc gtgtagagga acgccacctt gtgggccgga ccgctccccc
     8041 ggaagatgcc cctgccgcgc agggccctcc actcggccgg gttctccgcc gcaagggcgt
     8101 gcagcgccag cccggccttc tcgtggagct cggccccgtc gccgaagtcg atggcgacac
     8161 gctccggagc gcgaagctcg gcctcgggcg gcggccccac gacgggagcg cgtccggcac
     8221 cggcctcggt ctcgatcgat cgcagccggg tcatgagctc ggcggccgag ccggcaccga
     8281 cgaccagcgc tccccgcagg ggggacctgg gcacggccgg cccctcggcg gcacgtccgg
     8341 gcgccggccc ctcaccctga gtcaccgcga acgacttccg cccgcgcccg ttcgatgctc
     8401 cgggcacgta ctcgtccagg atcgcgtgga agttggttcc cccgaagccg aacgcgctca
     8461 ccccggcccg gcgggtgcca ccggcgggca cctcccactc ccgcagttcg gtgttcacgg
     8521 agaacggcga gtgggcccag tcgatgttcg ggttgggccg gtcgaagttg aggctgggcg
     8581 gcagcacctt ctcgtgcagc gcgaacaccg ccttgagcag cccggccgcg ccggctgctg
     8641 ccttcaggtg gccgatgttc gacttggcag atcccagggc gatcgagccc acgggcaggt
     8701 gaagcgggcc gaacgcctgg tggaggctct ccacctcgac cacgtcgccc agcgcggtcg
     8761 aggtgccgtg gccctcgatg agcccggccg tggccggcga cagcgcggca cgctcccagg
     8821 cccactccac ggccaggcgc tggcccacgg ggttcggcgc cgtcaggccc ctgcccttcc
     8881 cgtcgctcga tccggccagt cctcggatca ccgcgtggat ccggtcgccg tcccgttcgg
     8941 cgtcggccag gcgcttcagg aggaagacgg ccgctccctc gcccatgacg aacccgtcgg
     9001 cgccctcgtc gtacggccgg gtcccggtcg cggacag
//
LOCUS       DASPWO010000274         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_552374, whole genome shotgun sequence.
ACCESSION   DASPWO010000274 DASPWO010000000
VERSION     DASPWO010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552374"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2090
                     /locus_tag="VEO00_06240"
     CDS             <1..2090
                     /locus_tag="VEO00_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HYU57633.1"
                     /translation="EPSAPLPAAAPASGLVKRIVAATDRTHSADRACQVAASISGPIG
                     ADLVLLQVILASDSPDQDRAKAEAARHDLEAFAERLAGPRGTARVAIREDAAQGIVDE
                     ARDADADLLVVGNYGMAGRKQFLLKNVPNRISHMARCSVLIVNTTPVDEAETVVALGE
                     RTEQEPHAGKLLGRAVTIARTMSRHGVREMFTPVDPKDEDELRRRAKRLRTAMEELGP
                     AFGKLGQVMSTRPDLLPAPFVEELSTLQSDVPPLTEAQVVQVMEEELGVPWEDVFESI
                     EPEPLAAGTMAQVHGATLQDGEDVVVKVQRPNARHDILLDLGLLERFGQEIHERPLFR
                     EVVDLPAVIEQLSEALRQEMDFRQEADNIERLAEVLKPYPRLAVPKLFRDYCTDRLLV
                     MERVKGTRLREAPEGQARREAARQLLESYYRQIMTAGFFHADPHPGNLQWWNDKVYLL
                     DIGLVGRLEPETREQLMLLLLAFWQEDVPFLSDVVLMLSGDEARPDLDVEAFQDELSG
                     LLGRYRGKSLKEIQIGPLLQELTEISVRYRIRLPSSLTLTGKALAQVQLAISELDPTI
                     DPFSVAGNFMMKSMAGDLREKLSPQKLIYEGRKLGLRVSRLIEAVERIAGSRPGPNLQ
                     IRFRGAEPLERAIKQGARQLALAIVSLAALAGAAVAGGATNVPDWVAPAFGGAGGITT
                     VALIVDLIRSARR"
     gene            2154..3431
                     /locus_tag="VEO00_06245"
     CDS             2154..3431
                     /locus_tag="VEO00_06245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU57634.1"
                     /translation="MVDRSAGGGPAPIPASAGAPAGARPGSWWTLAAYVGLVASSHVL
                     WISFVSVSGQAARAFHTTEVSIGLLVSVGPLSSAALSIPAGALSDRYGYRTPLLWAGL
                     ATVAFAFLRPIAGAFPLLLLLTVGLLLPQPFLINAVADLVNRHFPEEEAATATGLGTM
                     AIFLGITVGLVATPALVEAVGVRGSQVAWAVMSLAALGAFWAAAPRRVPDRVAASEEL
                     SVREAIGRVLRSPTLWKLSAALFLGFGLYLGITTWLDQILKPKGIGESGAGLVAGMIT
                     IAGMAGSVALGALSDRIRRRKPFLVVAGAVAAPTLWLLGRLGSLPALVAVAFVLGFFL
                     LAALPVAISVASEDRSLGPQVGSTAVGVMLMAGNLGGALVVTVMGLLERPGNDFTAAV
                     ALASALALGVVVLAATVPEPLRRTEPVSADPAT"
BASE COUNT          447 a         1248 c         1309 g          511 t
ORIGIN      
        1 gcgaaccgtc cgcacccctc cccgcagccg ccccggcgag cggtttggtg aagcgcatcg
       61 tcgccgccac cgaccgcacc cacagcgccg accgcgcctg ccaggtggcc gcctcgatct
      121 cgggaccgat cggcgccgac ctggtcctgc tgcaggtgat cctggcgagc gactcgccgg
      181 atcaggaccg ggcgaaggcc gaggccgcgc ggcacgatct tgaggcgttc gccgagcggc
      241 tggccggccc gcgcgggacc gcgcgcgtgg cgatccgcga ggatgccgcg cagggcatcg
      301 tcgacgaggc gcgcgatgcc gacgccgacc tgctggtggt cgggaactac ggcatggccg
      361 ggcgcaagca gttcctgctg aagaacgtcc cgaaccgcat ctcgcacatg gcgcgctgca
      421 gcgtcctgat cgtgaacacc acgccggtcg acgaggccga gacggtggtc gccctcggcg
      481 agcgcacgga gcaggaaccg cacgcgggca agctcctcgg gcgcgcggtg accatcgccc
      541 ggacgatgag ccgccacggc gtccgcgaga tgttcacccc cgtcgacccg aaggacgagg
      601 acgagctgcg ccgccgcgcc aagcgcctgc gcacggcgat ggaggagctc ggcccggcgt
      661 tcggcaagct cggccaggtg atgtccaccc gcccggacct gctgccggcg ccgttcgtcg
      721 aggagctctc gacgctgcag agcgacgtcc cgccgctcac cgaggcccag gtggtccagg
      781 tgatggagga ggagctcggc gtgccctggg aggacgtgtt cgagagcatc gagcccgagc
      841 cgctcgccgc cgggacgatg gcgcaggtgc acggcgccac gctgcaggac ggcgaggacg
      901 tcgtggtcaa ggtgcagcgc ccgaacgccc ggcacgacat cctgctcgac ctcgggctgc
      961 tggagcgctt cggccaggag atccacgagc ggccgctgtt ccgcgaggtg gtcgacctgc
     1021 cggcggtgat cgagcagctc tccgaggcgc tgcgccagga gatggacttc cggcaggagg
     1081 cggacaacat cgagcggctg gcggaggtgc tgaagccgta tccgcgcctg gccgtcccga
     1141 agctgttccg cgactactgc accgaccggc tgctggtgat ggagcgcgtg aaggggacgc
     1201 gcctgcgcga ggcgccggag ggacaggcgc gccgggaggc cgcccgccag ctgctggagt
     1261 cctactaccg ccagatcatg acggccggct tcttccacgc cgacccccac cccggcaacc
     1321 tgcagtggtg gaacgacaag gtctacctgc tggacatcgg gctggtcggg cggctcgagc
     1381 cggagacccg cgagcagctg atgctcctcc tgctcgcgtt ctggcaggag gacgtcccgt
     1441 tcctctccga cgtcgtgttg atgctctccg gcgacgaggc ccgccccgac ctcgacgtcg
     1501 aggcgttcca ggacgagctc tccggccttc tggggcgcta ccgtggcaag tcgctgaagg
     1561 agatccagat cggccctctg ctgcaggagc tgaccgagat ctcggtgcgc taccgcatcc
     1621 ggctgccgtc ctcgctgacg ctcaccggga aggcgctggc ccaggtgcag ctcgcgatca
     1681 gcgagctcga cccgacgatc gacccgttct cggtggcggg caacttcatg atgaagagca
     1741 tggccggcga cctgagggag aagctgtcgc cgcagaagct gatctacgag ggccgcaagc
     1801 tcgggctgag ggtgtcgcgc ctgatcgagg ccgtcgagcg catcgccggc tcgcgtccgg
     1861 gcccgaacct gcagatccgg ttccgcggcg ccgagcccct cgagcgcgcc atcaagcagg
     1921 gcgcccgcca gctcgcgctc gcgatcgtct ccctcgccgc gctggccggc gcggccgtcg
     1981 ccggcggcgc cacgaacgtt cccgactggg tcgcccccgc attcggcggc gccggtggca
     2041 tcaccaccgt cgcgctgatc gtcgacctga tccgctcggc acggcgatag tcacgttccg
     2101 gtgtatcggc caggaggggt cgactgaccc ggtccggccg ggatgaggga gcgatggtgg
     2161 accggtccgc cggaggaggg cccgctccga tccccgcgtc cgccggcgcg ccggccggcg
     2221 cgcgcccggg ctcgtggtgg accctggcgg cctacgtggg cctggtggcg tcgagccacg
     2281 tgctgtggat ctcgttcgtc tcggtctccg ggcaggcggc ccgggccttc cacaccaccg
     2341 aggtgtccat cgggttgctg gtctccgtgg ggccgctctc ctccgcagcc ctgtcgatcc
     2401 ccgccggcgc actgtccgac cggtacggct accgaacccc gctgctgtgg gcgggtctgg
     2461 ccacggtggc gttcgcgttc ctccggccga tcgccggggc cttcccgctg ctcctcctcc
     2521 tgaccgtggg gctcctcctg ccgcaaccgt tcctgatcaa cgcggtggcc gacctggtga
     2581 accggcactt ccccgaggag gaggccgcca cggccacggg cctcgggacc atggccatct
     2641 tcctgggcat cacggtgggg ctggtggcca cgccggcgct ggtggaggcg gtgggcgtgc
     2701 ggggctcgca ggtggcgtgg gcggtgatgt cgctcgcggc cctgggggcg ttctgggccg
     2761 cagcgccccg gcgcgtgccg gaccgggtgg ccgcgtccga ggagctcagc gtgcgggagg
     2821 ccatcggacg ggtgctccgc tcgccgaccc tgtggaagct ttccgccgct ctgttcctcg
     2881 ggttcggcct ctacctcggg atcaccacgt ggctcgatca gatcctgaag ccgaagggga
     2941 tcggcgagtc gggcgccggg ctggtggccg ggatgatcac catcgccggg atggccggct
     3001 cggtggccct cggcgcgctg agcgaccgga tccgtcgacg gaagccgttc ctggtcgtgg
     3061 ccggggcggt cgcggcgccc acgctgtggc tgctggggag gctgggttcc ctgcccgccc
     3121 tggtcgcggt ggcgttcgtg ctggggttct tcctgctggc cgcgctgccg gtggccatct
     3181 cggtggcgtc cgaggaccgc tccctcggcc cgcaggtcgg aagcaccgcg gtgggggtga
     3241 tgctgatggc cggcaacctc ggcggggccc tggtggtcac ggtgatgggc ctgctcgagc
     3301 ggccggggaa cgacttcacg gccgccgtgg ccctggcctc cgccctggcg ctgggggtgg
     3361 tggtgctggc cgccaccgtt ccggagccgc tccgccggac cgaacccgtc tcggcggacc
     3421 cggcgacctg acccgggccc cggagcgacc cacccacgag tagcatgggc ccacggggga
     3481 gcccggcggg taccgggctg agagggcagg tgagg
//
LOCUS       DASPWO010000275         5427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_41374, whole genome shotgun sequence.
ACCESSION   DASPWO010000275 DASPWO010000000
VERSION     DASPWO010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41374"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VEO00_06250"
     CDS             <1..335
                     /locus_tag="VEO00_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYU57635.1"
                     /translation="PAGVLMYEVAGPLFFGAARQAMAALARTNKGVRVVVLDLRAVPA
                     MDVTGLVALESAIERLNHAQVFVVLAGVQRQPLRVMARAGWRDRHGKLAIYQSFERSL
                     DEVRKAFE"
     gene            480..1493
                     /gene="add"
                     /locus_tag="VEO00_06255"
     CDS             480..1493
                     /gene="add"
                     /locus_tag="VEO00_06255"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HYU57636.1"
                     /translation="MNDLLARLPKAELHLHLEGTISAETLWAMAQANHVALPVGSLPE
                     LRRLYHFESFDKFIELWLAMCSCFRAEADYERMVDAFLAECGRQNIRYAEVHFTPYNH
                     EKFGIGGRLSLEAVTRRLLASEAAGGPVVRIITDIPSESADASGPFTVALLEQEANPL
                     VVALGLGGPEAGHPRARFSRWFDRARSAGYPAVAHAGETGGAEHVRQAVLDLRVRRVQ
                     HGVRAVEDPEVLRLLAERGICCDVALTSNECLKVVPSLKAHPLRRMMAAGVPVTLSTD
                     DPPFFGTDLLREWERAQGELDLNVAELWELNLNGLRYGLADTALRRKLLREFETAGRG
                     FGL"
     gene            1635..2789
                     /locus_tag="VEO00_06260"
     CDS             1635..2789
                     /locus_tag="VEO00_06260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU57637.1"
                     /translation="MDFAESAEQRMLREAVAKIASGFGHGYYAEKARGGGKPTELWDA
                     LAGAGYLGVNLPEEYGGGGCGVTELAIVCEEMAAVGCSLFLVVVSPGICGSILARHGT
                     EEQKRRWLPGIADGSFKMAFAITEPDAGSNSHNLSTTADREGDGYRLRGQKYFISGVD
                     EADAILVVAKTGTDPGTGRGRLSLFVVDAGAPALKKTLIPVEMVSTDKQYTLFFDDVR
                     VGADRLLGTEGDGLRQAFTGLNPERITGAAAGLGLARYALDKAARYASQRKVWGAPIG
                     THQGLAHPLAQAKIDLELAKLMTYKAAWLFDTGQDAGEAANMAKFAAAETALFALDQA
                     IQTHGGNGLASEFGLADLWFVARLLRTAPVSREMVLNFVAEHSLGLPRSY"
     gene            complement(2796..4553)
                     /locus_tag="VEO00_06265"
     CDS             complement(2796..4553)
                     /locus_tag="VEO00_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HYU57638.1"
                     /translation="MPREPIRIANCSGFYGDRFSAAREMLEGGPLDVLTGDYLAELTM
                     FLLWRARERDPAGGYAATFLRQMEDVLGLALERGVRIVANAGGLNPAGLSARLRDLCA
                     RLGLRARVAHIEGDDLLARLPAVQAAGQALAHADTGRPLAEAGGRTVTANAYLGGWGI
                     AEALRGGADVVVGGRIADASLVVGPAAWHFGWARDDWDRLAGAAVGGHILECGPQATG
                     GNYAFFTEVPDLRHPGFPIAEVFADGSAVITKHPGTGGLVSVGTVTAQLLYEVEGPRY
                     VTPDVVARFDSAVVTPDGPDRVRISGTRGHPAPPTSKVGIQLLGGYRNGVTLVLTGLD
                     VDAKARVAVEALRPVLDACAQADVQLLRTDKPNAPACEEASAFLRLTVKDPDARKVGR
                     AFSGAVVELALASYPGFHATGPPEDASPYGIAWPALVDADLVEEVVVFDDGRRVRVPA
                     PPRAAAPPGLVPEAGVSAPPAGPTRRAPLGAAFGARSGDKGGNANVGVWARSDTGYAW
                     LAAQLTVERFKELVAEAAPLEVRRYLFPNLRAVNFVIVGLLGDGAAASSRLDPQAKGL
                     GEYLRSRLVDLPVSLLEAT"
     gene            complement(4560..>5427)
                     /locus_tag="VEO00_06270"
     CDS             complement(4560..>5427)
                     /locus_tag="VEO00_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYU57639.1"
                     /translation="ADGSVVSHHYDPLLAKLIVHASTRAQAAARLAAALERMRLHGVV
                     TNRDLLVGVLRHPEFLAGGTDTGFLDRHPPGTLVSAEAEAEAERLHAVAAALAGAAAR
                     RARAPVLAFAPAGWRNNPSQPQQAVFQGRRGRVEVSYRSARSGLTVAVDGRPLDVRLA
                     QATADVVVLENAGVRRRFEVHQVGEVAYVDSPLGHSALSEVDRFPSRATGTEAAGELV
                     APMPGVVVRLGARAGDEVERGAVVAVLEAMKMEHRVRAPHGGRVAEVRVREGQTIEAG
                     VVLAVIEQRRAG"
BASE COUNT          753 a         1939 c         2015 g          720 t
ORIGIN      
        1 tgcccgcggg cgtcctcatg tacgaggtgg cgggcccgct gttcttcggc gcggcccggc
       61 aggccatggc cgccctcgcc cgcaccaaca agggagtgcg ggtggtggtc ctggacctgc
      121 gcgcggtccc cgccatggac gtgaccgggc tggtggccct ggagtccgcc atcgagcggc
      181 tgaaccacgc ccaggtgttc gtggtgctcg cgggggtcca gcgccagcca ctgcgggtga
      241 tggcgcgggc gggctggcgg gatcgacacg gcaagctggc catctatcag tccttcgagc
      301 ggtccctcga cgaggtccgc aaagccttcg aatagcgcgg agccattcgt ggggcagcat
      361 tccctccgcc ctgcgtgggg gaggttagga gggggggtcg atgaatgaag ggcggagcca
      421 tacgtggggc agcattccct cccccctacg tgggggaggt taggaggggg ggtcgacgaa
      481 tgaacgatct cctcgcccgg ctgcccaagg ccgagctgca tctgcacctc gagggaacga
      541 tctcggcgga gacgctctgg gccatggcgc aggccaacca cgtggccctg cccgtgggct
      601 cgctcccgga gctgcggcgc ctctaccact tcgagagctt cgacaagttc atcgagctct
      661 ggctcgccat gtgctcctgc ttccgggcgg aggccgatta cgagcgcatg gtggacgcct
      721 tcctggccga gtgcggccgc cagaacatcc gctacgccga ggtgcacttc accccctaca
      781 accacgagaa gttcgggatc gggggccgcc tttccctcga ggcggtgacc cggcgcctgc
      841 tcgcgtccga ggcggcgggg gggccggtgg tccggatcat caccgacatc ccgagcgagt
      901 cggccgacgc gagcgggccc ttcaccgtcg ccctcctgga gcaggaggcg aacccgctgg
      961 tggtggctct cggcctggga gggccggagg ccggccatcc ccgcgcccgg ttcagccgct
     1021 ggttcgaccg ggcgcggagc gccggctacc cggcggtggc ccacgccggc gagaccgggg
     1081 gagccgagca cgtccggcag gcggtgctgg acctgcgggt ccgtcgcgtc cagcacggag
     1141 tccgcgcggt cgaggacccg gaggtgctgc gcctcctggc cgagcgcggg atctgctgcg
     1201 acgtggccct cacgagcaac gagtgcctga aggtcgtccc gtcgctgaaa gcgcacccgc
     1261 tgcggcggat gatggcggcc ggggtcccgg tcaccctctc caccgacgac ccgcccttct
     1321 tcggcaccga cctcctgcgg gagtgggagc gcgcccaggg ggagctcgac ctgaacgtgg
     1381 ccgagctgtg ggagctgaac ctgaacggcc tgcgctacgg cctcgccgac accgccctcc
     1441 ggcggaagct gctccgtgag ttcgagacgg cgggccgggg gtttgggctg tagacgggcg
     1501 gggcccgcac agcgcgctct gcgcgccacg cctgcctacg ccgcatcgtg gaagcgagcg
     1561 agcctcatcc ctgccgattg actccgagcc ggtggcgggc atagcttcgc gtcaggcttt
     1621 tccaggaggc caccatggac ttcgcggaga gcgccgagca gcggatgctt cgcgaggcgg
     1681 tcgccaagat cgcctcgggt tttggccacg gctactacgc cgagaaggcc cgcggcggcg
     1741 gcaagcccac cgagctgtgg gacgcgctgg ccggggccgg ctatctcggc gtgaacctac
     1801 ccgaggagta cgggggcggc ggttgcggcg tcaccgagct ggcaatcgtc tgcgaggaga
     1861 tggccgccgt cggctgctcc ctgttcctcg tcgtcgtctc tcccggcatc tgcggctcga
     1921 tcctcgcccg ccacggaacc gaggagcaga agcggcgctg gctgccgggc atcgcggacg
     1981 gaagcttcaa gatggccttc gccatcaccg agcctgacgc cggctccaac tcccacaacc
     2041 tctcgacgac ggccgaccgt gagggcgacg gctaccggct ccgcggccag aagtacttca
     2101 tctccggcgt cgacgaagcc gacgcgatcc tggtggtggc gaaaacgggg acggacccgg
     2161 gtaccggccg cggacggctc tcgctcttcg tcgtggatgc cggcgcgccg gcgctcaaga
     2221 agaccctgat tcccgtcgag atggtctcga ccgacaagca gtacaccctc ttcttcgacg
     2281 acgtccgcgt tggcgcggac cggctcctgg gcaccgaggg cgacggactg cgccaggcct
     2341 tcacgggcct gaacccggag cggatcacgg gcgccgccgc gggcctgggc ctcgcgcgct
     2401 acgctctcga caaggccgcc cggtatgcca gccagcgcaa ggtgtggggc gcgccgatcg
     2461 gcacccacca ggggctggcc catcccctgg cgcaggccaa gatcgacctc gagctggcga
     2521 agctgatgac ctacaaggcg gcctggctct tcgacacggg gcaggacgcg ggcgaggcgg
     2581 cgaacatggc caagttcgcc gcggccgaga cggcgctgtt cgcgctcgac caggcgatcc
     2641 agacccacgg cggcaacggc ctggcctcgg agttcggcct ggccgaccta tggttcgtgg
     2701 cgcggctgct gcggacagcc cccgtgagcc gcgagatggt cttgaacttc gtggccgagc
     2761 actcgctggg cctgccacgc tcctactgat cccggtcagg tggcctccag cagcgacacg
     2821 ggcaggtcca cgagccgtga gcgcaggtac tcgccgaggc ccttggcctg gggatcgagc
     2881 cgggacgagg cggcggcgcc gtcgccgagc agcccgacga tcacgaagtt gacggcgcgc
     2941 aggttcggga agaggtaccg gcgcacttcg aggggcgccg cttcggcgac gagctccttg
     3001 aagcgctcga cggtgagctg ggcggcgagc caggcgtagc cggtatcgga tcgcgcccac
     3061 accccgacgt tggcattgcc ccccttgtcg ccggagcggg cgccgaaggc cgccccgagg
     3121 ggggcgcggc gtgtcggccc ggcgggcggc gccgaaaccc ccgcctcggg caccaggccc
     3181 ggcggggccg cggcgcgggg cggtgcgggc acgcgcactc gccggccatc gtcgaagacg
     3241 acgacctctt cgaccaggtc ggcgtcgacc agggccggcc aggcgatccc gtacgggctc
     3301 gcgtcctcgg gcgggccggt ggcatggaag cctggatagc tcgccagggc cagctcgacc
     3361 accgcgccgg agaacgcgcg ccccaccttg cgggcgtcgg ggtccttgac cgtcaagcgg
     3421 aggaacgcgc tcgcctcctc gcaggcgggc gcgttcggct tgtcggtccg gaggagctgg
     3481 acatcggctt gagcgcacgc gtcgaggacg ggccggagcg cctccaccgc cactctcgct
     3541 ttggcgtcca cgtcgaggcc cgtgaggacg agggtgacgc cgttgcggta acccccgagc
     3601 agttggatcc ccaccttgga ggtgggagga gccgggtggc cgcgagtccc cgagatgcgg
     3661 acgcggtccg gcccgtcggg cgtgaccacg gcgctgtcga agcgggcgac gacgtcgggg
     3721 gtcacgtatc gcggcccctc cacctcgtag aggagctgcg cggtcacggt gccgaccgag
     3781 acgagcccac ccgtgcccgg atgcttggtg atgacggcgc tgccgtcggc gaacacctcg
     3841 gcgatcggaa agcccggatg gcgcaggtcg ggcacttcgg tgaagaaggc gtagttgccg
     3901 ccggtcgcct gcgggccgca ctcgaggatg tgccccccta cggcggcccc ggcgaggcgg
     3961 tcccagtcgt cccgtgccca gccgaagtgc cacgccgcgg gaccgacgac gagcgaggcg
     4021 tcggcgatcc ggcctcccac cacgacgtcg gcgccaccgc ggagcgcctc tgcgatcccc
     4081 cacccgccca ggtacgcgtt ggccgtcacg gtgcgccctc cggcttcggc cagggggcgg
     4141 cccgtgtcgg cgtgggccag ggcctggccg gcggcctgca cggccggaag ccgcgcgagc
     4201 aggtcgtccc cctcgatgtg ggcgacgcgg gcccgcaggc ccagacgggc gcagagatcg
     4261 cgcaggcgcg cggagaggcc ggcggggttc aggcctcccg cgttggccac gatgcgcacc
     4321 ccccgctcga gggctaggcc gaggacgtcc tccatctgtc tgaggaacgt cgctgcgtag
     4381 ccgcccgccg gatccctctc ccgcgcgcgc cagaggagga acatggtcag ctcggcgaga
     4441 tagtccccgg tgaggacgtc gagagggccg ccctcgagca tctcccgagc ggcggagaag
     4501 cggtcgccgt agaagcccga gcagttggcg atgcggatcg gctcgcgcgg catcgcggct
     4561 cagcccgctc gccgctgctc gatcacggcc agcacgaccc cggcctcgat cgtctggccc
     4621 tcccgcaccc gcacctcggc cacgcggccg ccgtgcggcg ccctcacacg gtgctccatc
     4681 ttcatggcct cgaggacggc cacgaccgct ccccgctcca cctcgtcgcc ggcgcgcgcc
     4741 ccgagccgca cgacgacgcc cggcatgggc gccaccagct cgcccgcggc ctcggtgccc
     4801 gtcgcccggg acgggaaacg gtcgacctcg ctcagggcgg agtggccgag ggggctgtcg
     4861 acgtaggcga cctcgccgac ctggtggacc tcgaagcggc ggcgcacgcc cgcgttctcg
     4921 aggacgacga cgtcggccgt ggcctgggcg agacgcacgt cgaggggccg gccgtccacc
     4981 gcgacggtga gacccgaacg cgcggaccga tagctcacct cgacgcgtcc gcggcggccc
     5041 tggaagacgg cttgctgcgg ctgcgaggga ttgttgcgcc agcccgcggg ggcgaaggcg
     5101 agcaccggcg ctcgcgcccg gcgggccgcc gcccccgcca gggccgcggc cacggcgtgg
     5161 agccgctcgg cctcggcctc cgcctcggcc gagacgaggg tccccggcgg gtggcgatcg
     5221 aggaaaccgg tgtcggtccc gccggcgagg aactccggat gacgcaggac gcccacgagc
     5281 aagtcgcggt tggtcacgac cccgtggagc cgcatccgct cgagggccgc ggccagcctc
     5341 gccgcggcct gggcccgggt cgaagcgtgg acgatgagct tcgcgaggag cgggtcgtag
     5401 tggtggctca cgaccgagcc gtcggcg
//
LOCUS       DASPWO010000276         2685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_41484, whole genome shotgun sequence.
ACCESSION   DASPWO010000276 DASPWO010000000
VERSION     DASPWO010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41484"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VEO00_06275"
     CDS             complement(<1..878)
                     /locus_tag="VEO00_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYU57640.1"
                     /translation="MSLRRAVLVAAAAILALLGFGAQGAEATEHCGKANAPGGEWPSY
                     GHDYANTRYQQQEHVISPADVPLLSPAWTVSTVDAGGKGDITGTPIVAFGCVYVATNR
                     GWAFAFNADTGKRVWKASVPYGGGVSSSVGVTAGRVYLSVTRTQQAKGCPKGDPCVGP
                     YVVALDRRTGRLAWATRPIDRQPGADVFGSPVTFDGVVMIGVSGGAAELGDEADRYAF
                     QGSMNFLDARTGRLLRKTWTIHRPNHPKDDFAGAGIWSTPAIDRRAKVAYVGAGNPFR
                     PQAEHKHADAVLKVDV"
     gene            complement(875..2563)
                     /locus_tag="VEO00_06280"
     CDS             complement(875..2563)
                     /locus_tag="VEO00_06280"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYU57641.1"
                     /translation="MPCSNEEYLPPPPSKRQLAIMRLADAETERWRRKFSMSRAQFVR
                     TAAATAIGFWAIDALSPGLWGNYGWAHNTATTDACDLQWDGKKGLQTLHNLAGEFIFD
                     VQSHHVDPDGMWRVTNPAIHAFFAAVWPQSSAATGDQPGVREDGSIRGGGAGEIDPIE
                     NLSRYHYLKELFLDSATTMTVLSCVPTSPDTNNPLPLAEAALTVHTVNDLARSQRSIM
                     HAFVMPNRGSAGHNSPNMKPLYLDEELQLMMERAHQYRGILRGWKTYCPWGDVPNASG
                     WFLDSDTGMAFLEQVRKVSKAVPEVPPLVASHKGFALPGFDQSAATPRDIGPAARQFP
                     DVNFLVYHSGHDIGDAQRRYAGDAKARSDSNTVDALIKSLRENDYDSSRFRRKGQRFG
                     NVPNVYAELGSVWRDVMHDPDQCAHLLGKLIKYVGPKRVAWGTDSLWYGSPQAEIVAL
                     RRFEFSERAKEFYGLPHGLEGDVEDPTRKAPRPERTIRNAILGRNAARAYRIDPDARR
                     HEIACDAVNGLRQHGYLSGAGTELESAPLASNTAPGPRTRREVLKALRENPWSP"
BASE COUNT          370 a          923 c          937 g          455 t
ORIGIN      
        1 tcgacgtcga ccttcagcac cgcgtcggcg tgcttgtgct ccgcttgcgg gcggaacggg
       61 ttgccggctc cgacgtaggc gaccttcgcc cggcggtcga tcgcgggggt cgaccagatg
      121 cccgcgcccg cgaagtcgtc cttggggtgg ttcggtcggt gaatggtcca ggtcttgcgc
      181 agcaaccgcc ccgtgcgggc gtccaggaag ttcatcgagc cctggaacgc gtagcggtcg
      241 gcctcgtccc ccagctcggc ggcacccccg ctgaccccga tcatcacaac gccgtcgaag
      301 gtcaccgggc tgccgaaaac gtcggctccg ggctgcctgt cgatcggtcg ggtcgcccac
      361 gccaatctcc cggtgcgccg gtcgagggca acgacatacg ggcccacgca ggggtcgccc
      421 ttcggacagc ctttcgcctg ctgcgtgcgg gtcacggata gatagacgcg ccccgccgtc
      481 acccccaccg agctgctgac gccccccccg tagggcaccg aagccttcca tacccgcttg
      541 cctgtgtcgg cgttgaaggc gaacgcccac ccgcggttcg tcgccacgta gacgcagccg
      601 aacgcgacga tcggagtccc ggtgatgtcg ccctttccgc cggcgtcgac cgtcgacacg
      661 gtccaggcgg gggacagcag gggcacgtcc gccggcgaga tcacgtgctc ctgctgctgg
      721 taccgggtgt ttgcgtaatc gtgcccgtag ctcggccact cgcctccggg cgcgttggcc
      781 ttgccgcagt gctccgtcgc ctccgctccc tgcgccccga acccgagcag cgccaggatt
      841 gcggcggcag cgacgagaac ggcacggcgc aggctcatgg agaccagggg ttttcccgca
      901 gcgccttgag aacctcgcgg cgggtgcggg ggccaggcgc cgtgttcgag gcgaggggtg
      961 cggactcgag ctccgttccc gcaccgctca ggtagccgtg ttgccggagc ccgttcacgg
     1021 cgtcgcaggc gatctcgtgc cggcgggcgt cgggatcgat ccggtaggcc cgcgcggcgt
     1081 tgcggccgag gatcgcgttg cggatcgtcc gctcgggccg cggggccttg cgggtcgggt
     1141 cctcgacgtc gccctcgagg ccgtgcggga gcccgtagaa ctccttggcg cgctcgctga
     1201 actcgaagcg gcgcagtgcg acgatctcgg cctgcggtga cccgtaccac aggctgtcgg
     1261 tgccccaggc gacccgcttc gggcctacgt acttgatcag cttgcccagc aggtgggcgc
     1321 actggtccgg gtcgtgcatg acgtcgcgcc acacggatcc gagctccgcg tacacgttcg
     1381 ggacgttgcc gaacctctgg cccttgcgcc tgaatctgct cgagtcgtag tcgttctcgc
     1441 gcaggctctt gatcagcgcg tcgaccgtat tcgagtcgga gcgggccttg gcgtctcccg
     1501 catagcgccg ctgcgcgtcg ccgatgtcgt gccccgagtg gtagacgagg aagttgacgt
     1561 cggggaactg gcgcgcggcg ggcccgatgt cgcggggggt ggcggcgctc tggtcgaatc
     1621 cgggcagcgc gaaccccttg tgcgaggcga ccagcggtgg cacctccggc acggccttgc
     1681 tgaccttgcg gacctgctcc aggaaggcca tcccggtgtc gctgtccagg aaccagccgc
     1741 tggcattggg gacgtctccc cacgggcagt aggtcttcca gccgcggaga attccgcggt
     1801 actggtgggc ccgctccatc atcagctgga gctcctcgtc gagatagaga ggcttcatgt
     1861 tcgggctgtt gtggcccgcg gagcctcggt tgggcatcac gaacgcgtgc atgatcgatc
     1921 gctgggagcg cgccaggtcg ttgacggtgt gcaccgtgag cgccgcctcc gcgagcggga
     1981 gcgggttgtt cgtgtcgggc gacgttggta cgcacgacag cacggtcatc gtcgtcgccg
     2041 agtcgaggaa cagctccttc aggtagtggt agcgcgacag gttctcgatc gggtcgatct
     2101 ctccggcccc gccgccgcgg atcgagccat cctcgcggac gccgggctga tcgcccgtgg
     2161 ccgccgagga ctgcggccac acggcggcga agaaggcgtg gatcgccgga ttggtgaccc
     2221 gccacatgcc gtccgggtcg acgtggtgtg actgaacgtc gaagatgaac tcaccggcga
     2281 ggttgtgcag ggtctggagt cccttcttgc cgtcccactg caggtcgcag gcgtccgtcg
     2341 tggcggtgtt gtgggcccag ccgtagttgc cccacaaccc ggggctcaac gcgtcgatcg
     2401 cccagaaccc gatcgccgtc gccgcggcgg tgcgcacgaa ctgcgcgcgg gacatgctga
     2461 acttccggcg ccaacgctcc gtctccgcgt ccgcgagcct catgatcgcc agctgcctct
     2521 tgctcggcgg aggcgggaga tactcctcgt tcgagcacgg catcacgtcc accggcaggt
     2581 cctcggagag gtccggccac gagaagcgct cgggtccgga ggtaagcgaa ggccctgact
     2641 gcctccagtc gatcacttca cgacctcgaa cgctccccgc atcga
//
LOCUS       DASPWO010000277         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_890034, whole genome shotgun sequence.
ACCESSION   DASPWO010000277 DASPWO010000000
VERSION     DASPWO010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890034"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VEO00_06285"
     CDS             <1..643
                     /locus_tag="VEO00_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57642.1"
                     /translation="STPAEFEEWMKAEERSRVDFHELNRWAVARRAETAVISLALEWG
                     RAADLLLARAAKADKAAWTGRVVWPAEEIPHHLFVQSRVAEWWAHGEDIRAATGLEAR
                     IEHPPIYCVNDLSIRTIPYGLGLAGLSFPGRSVRIDLTGAGGGSWHHGLAAGDRPAPD
                     KAPDAYIEGRAHAFALVAEQHADPDEFLYDGLLVLGGDTGLARTVLRHVRTYI"
     gene            695..1291
                     /locus_tag="VEO00_06290"
     CDS             695..1291
                     /locus_tag="VEO00_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU57643.1"
                     /translation="MGRLRRAARSAITVNDRSGADLMRGFMRREGRAAEELYAEFAPR
                     IFGLGMVILGNPSAAEDLVQDTMVKMWRNAERFDPARGSLETWVMRMARNLAIDAVRR
                     RVLESRHTDRSAAAEGPDPDPGPEHVAEVTDLVGRARAAMRSLSDEQRVALELAYFGG
                     RTAAEVAQMEGVPLGTVKTRIRRALLSLRDTLEVRRDL"
     gene            1281..2015
                     /locus_tag="VEO00_06295"
     CDS             1281..2015
                     /locus_tag="VEO00_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYU57644.1"
                     /translation="MTCEGVRDRLPDLLLGHLGEVDEAGVRRHLRACGPCRRDLAALE
                     EGLAVFSRAAHTVEPPAELRERVLAAVEEEWTATGRRSSSGRARRAVLAALGAAALVA
                     GIAGATWGVLAERRADRLDAAAAVLRADAGRYREFLDALGGRAVRVARLASPSDPEIH
                     GSAVVYIGDEGPSWILLYVGGADPGQGAHVELVGDRRTIQAGRLQLDEDGSGSVYWVT
                     RRDLSWQHTVRVVDADGRVLALGKIA"
     gene            complement(2026..>2920)
                     /locus_tag="VEO00_06300"
     CDS             complement(2026..>2920)
                     /locus_tag="VEO00_06300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU57645.1"
                     /translation="WFHTPQFLKTWWEEFHGDAELRLAFVQDGGETVAACAFEVRNGL
                     LAFLGGFDVTDYLGPVGRPGVEDGAAAALADAVAGLGWARADLAGLVSDGRWLPALTR
                     AFERAGLLPDEDDDGVTPLLVLPSTYEAYEASLPPKLRHEARRKARKLEREAGPYRLV
                     ETTRDTLDAHVGRFVELHRGSKGPKGRFMQPGMELFFRHLADDFLAPGPFRLTFIEAA
                     GELMAGAISFRFKESVLLYNSAFDRAHRALSPGMVLVADMIRQAIADGCRRFDLLKGD
                     LGYKYRFGARPRPVRRLRLSR"
BASE COUNT          388 a         1070 c         1094 g          368 t
ORIGIN      
        1 atctacgccc gcggagttcg aggagtggat gaaggccgag gagcgctcgc gggtcgactt
       61 ccacgagctc aaccgctggg ccgtggcacg ccgcgccgag acggcggtca tctcgctggc
      121 actggagtgg ggacgcgccg ctgacctgct gctggcccgg gccgcgaagg ccgacaaggc
      181 ggcctggacc gggcgggtgg tctggccggc ggaggagatc ccccatcacc tgttcgtcca
      241 gtcccgcgtg gcagagtggt gggcgcacgg cgaggacatc cgcgcggcga ccgggctcga
      301 ggcacggatc gagcacccgc cgatctactg cgtgaacgac ctctcgatcc ggacgatccc
      361 ctacggtctc ggcctggcag gcctctcctt ccccgggcgc agcgtgcgga tcgacctcac
      421 cggcgccggc ggcggctcgt ggcaccacgg cctggccgcg ggcgaccgtc cggcgcccga
      481 caaggcgccg gatgcctaca tcgagggccg ggcgcacgcg ttcgcgctgg tcgcggagca
      541 gcacgccgac ccggacgagt tcctgtacga cggcctgctg gtgctcggcg gcgacaccgg
      601 cctggcgcga acagtcctcc ggcacgtccg cacctacatc tgagcggaga ccggtggcga
      661 atcccggccg ccgcggcccg cgtacttcct acggatggga cggctgcgcc gggccgcacg
      721 gagcgccatc accgtgaacg accggtcggg cgccgacctg atgcgcgggt tcatgcggcg
      781 ggagggccgc gcggcggagg agctgtacgc ggaattcgca ccgcgcatct tcggcctcgg
      841 catggtcatc ctcggtaacc cgtcggcggc ggaggacctg gtgcaggaca cgatggtcaa
      901 gatgtggcgg aacgcggaac ggttcgaccc ggcacgcgga tcgctggaga cctgggtgat
      961 gcggatggca cggaacctgg ccatcgacgc ggtgcgccgg cgggtgctgg agagccggca
     1021 caccgaccga tccgccgcgg ccgaggggcc cgaccccgat cccggacccg agcacgtggc
     1081 cgaggtcacc gacctggtcg gccgggcgcg cgccgcgatg cgctcgctct cggacgagca
     1141 gcgggtcgcc ctggagctcg cctacttcgg cggccgcact gccgccgagg tggcgcagat
     1201 ggagggcgtc cccctcggca cggtgaagac gcgcatccgc cgggcgctgc tctcgttgcg
     1261 cgacacgctg gaggttcgcc gtgacctgtg agggggtccg cgaccggctg cccgacctgc
     1321 tgctcgggca cctgggcgaa gtcgatgagg ccggcgtgcg ccgccacctg cgcgcgtgcg
     1381 gcccgtgccg gcgcgacctc gccgcgctcg aggaaggcct ggccgtcttc tcgcgcgcgg
     1441 cgcacacggt cgagccgccc gcggagctgc gcgagcgggt gctcgccgcc gtggaggagg
     1501 aatggacggc gaccgggcga cggtcgtcgt ccgggagggc ccggcgggcc gtcctggccg
     1561 cgctgggcgc cgcggcgctc gtcgccggga tcgccggcgc gacgtggggc gtcctggccg
     1621 agcggcgcgc cgaccgtctg gacgccgcgg ccgcggtcct gcgggccgac gccggccgct
     1681 atcgggagtt cctcgacgcg ctcggcggac gcgccgttcg cgtcgcccgg ctcgcatcgc
     1741 cgtccgaccc ggagatccac gggagcgcgg tcgtctacat cggtgacgag gggccctcgt
     1801 ggatcctgct gtacgtcggc ggtgccgacc ccggccaggg cgcgcacgtc gagctggtcg
     1861 gcgaccgccg cacgatccag gccgggaggc tgcagctcga cgaggacggc agcgggtccg
     1921 tgtactgggt cacccgccgc gacctctcct ggcagcacac cgtccgcgtc gtggacgcgg
     1981 acgggcgcgt gctggccctc ggcaagatcg cctgagcgga cgagctcatc gggacagccg
     2041 cagccggcgc accggccgcg ggcgcgcgcc gaaccggtac ttgtaaccga gatcgccctt
     2101 cagcaggtcg aagcggcggc acccgtccgc gatggcctgc cggatcatgt cggccaccag
     2161 caccatcccc ggcgagagcg cccggtgggc gcgatcgaag gccgagttgt agaggagcac
     2221 gctctccttg aaccggaagg agatcgcccc ggccatcagc tcgccggccg cctcgatgaa
     2281 cgtcaggcgg aacggccccg gcgccaggaa gtcgtcggcc aggtggcgga agaagagctc
     2341 catgcccggc tgcatgaacc tccccttcgg gcccttgctg ccgcggtgca gctcgacgaa
     2401 ccggccgacg tgcgcgtcga gggtgtctcg cgtcgtctcg accagccggt acgggcccgc
     2461 ctcgcgttcc agcttgcgcg ccttgcgccg cgcctcgtgg cgcagcttcg gcggcagcga
     2521 cgcctcgtag gcctcgtacg tggacggcag gacgagcagc ggcgtgacgc cgtcgtcgtc
     2581 ctcgtccggc agcagtccgg ctcgctcgaa cgcccgcgtc agggcgggaa gccaccgtcc
     2641 gtccgagacg agcccggcca ggtcggcgcg ggcccatccc agcccggcga cggcatccgc
     2701 gagcgccgcc gccgcgccgt cctccacgcc gggccgcccg acggggccca ggtagtcggt
     2761 gacgtcgaac ccgccgagga acgccagcag cccgttgcgc acctcgaacg cgcaggcagc
     2821 cacggtctcg ccgccgtcct gcacgaacgc caggcgcagc tccgcgtcgc cgtggaactc
     2881 ctcccaccac gtcttcagga actgcggggt gtggaaccag
//
LOCUS       DASPWO010000278         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_123357, whole genome shotgun sequence.
ACCESSION   DASPWO010000278 DASPWO010000000
VERSION     DASPWO010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123357"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..618
                     /locus_tag="VEO00_06305"
     CDS             262..618
                     /locus_tag="VEO00_06305"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HYU57646.1"
                     /translation="MSGGDWALIVGAAFWALLVLFISLVLVNVFRVLESVKMMIDGVR
                     TETVPMLSEVTESVKRVNRELDRVDGVLVSAGKISRSVERLSTVVENTVSSPLIKVAA
                     FSAGAARAVRRLRGEK"
     gene            615..845
                     /locus_tag="VEO00_06310"
     CDS             615..845
                     /locus_tag="VEO00_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57647.1"
                     /translation="MRRLFTLAVGIGAGAAVAIVVTRWARRKREAVTAKAIVGRASEG
                     ARDLAALFRSAIDEGRKAMAAREAEIRALLPE"
     gene            874..>3466
                     /gene="alaS"
                     /locus_tag="VEO00_06315"
     CDS             874..>3466
                     /gene="alaS"
                     /locus_tag="VEO00_06315"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HYU57648.1"
                     /translation="MESAAIRDAFLRFFEERGHTRVPSSSLIPNDPTLLLTTAGMVQF
                     KPYFLGEQEPPWPRATSVQKVFRTTDIENVGRTIRHNTFFEMLGNFSFGDYFKEGAIA
                     HAWDLVTEVYGLDPDSLWATVFEQDEESEALWGAYLPAARIVRRGKADNYWSMGVAGP
                     CGWCSEIYVDRGPEFGPEGGPAVDEERFLEIWNLVFMQFLRDDDDNIVGPLPRTGVDT
                     GSGLERVAIVLQKAPSIYETDLLAPVLEVAQSVTGRTYGRDHRDDVSLRILAEHGRAT
                     TFLIADGVLPSNEGRGYVLRRMLRRVVTHARRLGVETTVTVPLAERTIELMGDHYPEL
                     AENRAFVLQVAATEEERFARTLRQGLVVFEDERARLDDRTTLPGEAAFRLHDTYGFPL
                     ELTLELAEEAGLAVDRDGFAELMERQRAQARGALERRREDVDAALAPLSAGLGPTRAL
                     AYEATEAEGTLQAILRGPEAVEMAGEGEAVHVVLDRTPFYAEGGGQVGDAGTIVTDTG
                     RLEVEDTRPGPGGVIVHRARVAAGEVLAGQAARAEVDRPRREATMRSHTATHVLHWTL
                     RHLLGEHARQAGSLVAPGRLRFDFTHFAGLSPEELEAAELEANRRLAEDAPVRPYETT
                     YEYARGRGAIALFGEKYGDVVRVVEIGDWSIELCGGTHVARTGNVAMLRLLHESSVGS
                     GLRRVEAVVGPDAIAHVNQERRLLEEVVEALGGGDPAQAPERIRRTMDRLKDLENELG
                     RLRAEQRGAEQASLLEGAVDVAGTALVVADVPADADELRQRALALRDALAGKPAAIVL
                     GHADDRRALLVASVTDALRDLRVTAPALLAEAATRVGGGAGGKDHLAFAGGGKPGELA
                     AALASIPD"
BASE COUNT          473 a         1191 c         1339 g          463 t
ORIGIN      
        1 agccccccgt ggcggtatgc tcacgggcca tgacggagcg tgcgcgcagg accccgtcta
       61 tcttcatccg caactggcgg gattcggacg acccgttctg gacgaaggtc ggccagttcg
      121 tcacgaaccg ggcgcgcaag cccgggaggc tgtgctgcgg ccacccgggt caacccgggt
      181 gttgagaggc ccgtccggac acgggccccg gtcggggccc gcacgaccac ggggacgggg
      241 atctctgaga ggaggggagc cgtgtcggga ggcgactggg cgctgatcgt cggggccgcg
      301 ttctgggcgc tgctggtgct cttcatctcg ctcgtcctgg tgaacgtgtt ccgggtgctc
      361 gagagcgtga agatgatgat cgacggcgtc cgcaccgaga cggtcccgat gctgtccgag
      421 gtgaccgagt cggtgaagcg ggtcaatcgc gagctcgacc gcgtggacgg cgtgctggtt
      481 tcggccggca agatctcgcg gtccgtcgag cgactctcga cggtggtcga gaacacggtg
      541 tccagccccc tgatcaaggt ggccgccttc tccgccggcg ccgcgcgggc ggtccggcgg
      601 ctgcgaggag agaagtgagg cgcctgttca cgctcgccgt cggcatcggc gccggggccg
      661 cggtggcgat cgtcgtcacc cgctgggccc ggcgcaagcg cgaagccgtg acggcgaagg
      721 cgatcgtcgg ccgggcctcc gaaggggcac gcgacctcgc cgctctcttc cgctcggcga
      781 tcgacgaggg ccgcaaggcg atggccgccc gcgaggccga gatccgcgcg ctgctgcccg
      841 agtaggccca tcccgctccc gtagactcgc gccgtggaga gcgcggcgat ccgggacgcc
      901 ttcctgcggt tcttcgagga gcgcgggcac actcgggtgc cctcttcgtc gctgatcccc
      961 aacgacccga cgctcctgct gaccaccgcg gggatggtgc agttcaagcc gtacttcctc
     1021 ggcgagcagg aaccgccctg gccgcgggcc acgtccgtgc agaaggtgtt ccgcaccacc
     1081 gacatcgaga acgtcgggcg gacgatccgc cacaacacgt tcttcgagat gctcgggaac
     1141 ttcagcttcg gcgactactt caaggagggg gcgatcgccc acgcctggga cctggtgacc
     1201 gaggtctacg gcctcgaccc cgacagcctg tgggcaaccg tgttcgagca ggacgaagag
     1261 tccgaggcgc tctggggagc gtacctgccg gccgcccgga tcgtccgccg cggcaaggcc
     1321 gacaactact ggtcgatggg ggtggccggg ccgtgcggat ggtgctcgga gatctacgtg
     1381 gaccgcgggc cggagttcgg gccggaggga ggcccggcgg tcgacgagga gcggttcctc
     1441 gagatctgga acctcgtgtt catgcagttc ctccgggacg acgacgacaa catcgtcggc
     1501 cctctgccga ggaccggggt ggacaccggc tcgggcctcg agcgcgtcgc gatcgtgctg
     1561 cagaaggccc cctcgatcta cgagaccgac ctgctggcgc cggtgctcga ggtggcccag
     1621 tcggtgaccg gccggacgta cgggcgcgac caccgcgacg acgtctcgct gcgtatcctc
     1681 gccgagcacg gccgggccac gacgttcctg atcgccgacg gcgtgctgcc gtcgaacgag
     1741 gggcgcggct acgtgctgcg gcgcatgctc cgccgcgtgg tcacccacgc gcgccgcctc
     1801 ggcgtcgaga ccacggtcac cgtcccgctc gccgagcgga cgatcgagct gatgggcgat
     1861 cactatcccg agctcgccga gaaccgtgcg ttcgtcctgc aggtcgcggc gaccgaagag
     1921 gagcgcttcg cccggaccct gcgccagggg ctggtggtct tcgaggatga gcgggcccgg
     1981 ctggacgacc ggacgacgct cccgggcgag gcggcgttcc gcctgcacga cacctacggc
     2041 ttcccgctcg agctgaccct tgagctggcc gaggaggcgg gtctcgcggt cgaccgcgac
     2101 gggttcgccg agctgatgga gcgtcagcgc gcgcaggccc gcggggcgct cgaacggcgg
     2161 cgtgaggacg tggatgccgc gctggcgccg ctgtcggcgg gactggggcc gacccgcgcg
     2221 ctggcctacg aggccaccga ggccgagggc acgctgcagg cgatcctgcg cggccccgag
     2281 gccgtcgaga tggccggcga gggcgaggcg gtgcacgtgg tgctcgaccg gacgccgttc
     2341 tacgcggagg gcggcggcca ggtgggggac gccggaacga tcgtcaccga caccgggagg
     2401 ctggaggtcg aggacactcg gccgggcccg ggcggagtga tcgtccaccg tgcccgcgtc
     2461 gcggccggcg aggtgctcgc cggacaagcg gctcgagccg aggtggaccg gccccggcgc
     2521 gaggcgacga tgcgctcgca caccgccacc cacgtgctgc actggacgct gcgccacctg
     2581 ctcggcgagc atgcccggca ggcggggtcg ctcgtggcgc ccgggcgtct gcgcttcgac
     2641 ttcacgcact tcgcgggcct ctcgccggag gagctcgagg ccgccgagct ggaggccaac
     2701 cgacggctgg ccgaggacgc gccggtccgg ccgtacgaga ccacgtacga gtacgcgcgg
     2761 ggccgcggcg ccatcgcgct gttcggcgag aagtacggcg acgtggtccg cgtggtcgag
     2821 atcggcgact ggtcgatcga gctgtgcggc ggcacccacg tcgcgcggac cgggaacgtc
     2881 gcgatgctcc ggctgctgca cgagtcgagc gtcggctcag ggctgcggcg ggtcgaggcc
     2941 gtggtcgggc ccgacgcgat cgcccacgtc aaccaggagc ggcgcctgct ggaggaggtg
     3001 gtcgaggccc tcggtggcgg cgaccccgcc caggcgccgg agcggatccg gcggaccatg
     3061 gaccggctca aggacctgga gaacgagctc ggccggctgc gcgccgagca gcgcggggcc
     3121 gagcaggcgt cgctgctcga gggggccgtg gacgtcgccg ggaccgcgct ggtggtcgcc
     3181 gacgtgccgg cggacgccga cgagctccgg cagcgggcgc tcgcactacg cgacgccctg
     3241 gccggtaagc ccgccgcgat cgtgctcggc catgccgacg accggcgcgc gctgctggtg
     3301 gcgtcggtga ccgacgcgct gcgggacctg cgggtgaccg ccccggccct gctggcggag
     3361 gccgccacac gggtcggagg gggcgcgggc gggaaggatc acctggcctt cgccgggggc
     3421 gggaagccag gcgagctggc cgcggccctc gcctcgatcc ccgacc
//
LOCUS       DASPWO010000279         6887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_463493, whole genome shotgun sequence.
ACCESSION   DASPWO010000279 DASPWO010000000
VERSION     DASPWO010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463493"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1654)
                     /locus_tag="VEO00_06320"
     CDS             complement(26..1654)
                     /locus_tag="VEO00_06320"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HYU57649.1"
                     /translation="MLEFVHEEFSDEERRLLAPYVTNLDGPVFALANLSETVKGALFA
                     RYSRSPKSLRRLFLDEFAEPAERSAGAAEAAVGERHAEELYGRIFFEYGDDSVAQLGG
                     VHLACEQASQLLGRVLERGRLAAYLEQSTRYVPYDDRPAGRWRFRTPSELEDTALAAP
                     YRAALDRAFETYARWLGPLQAFFRERFPRQAEDSDFVYRMTIRAKACDTLRGLLPAAT
                     VSNVGIFATGQSYEHLLLRMRAHPLREVREYGDLMLVELRKVIPAFLRRVDLEGRGMA
                     WSRYLEERRKATAERAETFLADVPDPPRPLVTLTDFDPDGELKVTAACLYEETHLPDD
                     VLLERVRALSAGERGAILSAAVGTRENRRHRPGRAFERTGYRFEVLCDYGAFRDLQRH
                     RPLTIQWQELTPRHGYETPPEVDEAGARAEWDGVMAECADLSTRIREAGLGPAAQYPV
                     PMAHRIRFVMQMNAREAMHLIELRSSPQGHPAYRSVAHELHRMIGEVAGHRGIAAAMR
                     HVDRSAVDLERLAAERRTDERRRARRAGTAGSSP"
     gene            complement(1704..3551)
                     /gene="pknB"
                     /locus_tag="VEO00_06325"
     CDS             complement(1704..3551)
                     /gene="pknB"
                     /locus_tag="VEO00_06325"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HYU57650.1"
                     /translation="MLGGRYEVHAPLGEGGMATVFRGVDTVLGRPVAVKVLAPRYAND
                     ATFVARFRREAQSAAAMNHPNVVNVYDTGSDRGVHYIVMELVEGRTLREVIREEGPLL
                     PERAAEIAAAVCQALAFAHERGIVHRDVKPGNIMLTPTGEVKVMDFGIARATSGEALT
                     QTATVLGTASYISPEQAQGESVDARSDIYSLGVVLYEMLTRQVPFSGDNPVAIAYKHV
                     QEEAIRPSRLNADVPQPLESIVLKALAKNPANRYQSAAEMLADLQRFLAGQSVQATPL
                     LPPEPTEVIAREARETLVLPATEAGAGPAGRSAGRTAAFVIAVILLLATLGVLFFLLA
                     RQIIGDGDVAQVTVPNCVNRRLDACRLLLRQEGLKANVSERRANPDIPAGVVIEQDPA
                     ADDTADEGATVDLVVSNGPVEVEVPDLTGKTEDQARQTLEDNDLTLGSVDRAFSPDVE
                     EDHVFRQDPGAGENVPQGSAVDITLSAGQEPVDVPALTGLSPDDAQQVLETAGLRLGT
                     VEEGTCTSDGGPGTICAQDPDPNVQVNPDTEVDVTVQPQNIEVPDLTGMTETEAKQAL
                     KDGGLQLGDTTTGGATCISPNGSGTVCAQEPDDGTQVPPGTSVDIALQA"
     gene            complement(3569..4867)
                     /locus_tag="VEO00_06330"
     CDS             complement(3569..4867)
                     /locus_tag="VEO00_06330"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYU57651.1"
                     /translation="MSPPATESLARGRYRLSERLASGGMGAVWRGEDIELGRPVAVKL
                     LHEGLLDDPSSRERFRREARAAAALAHPNIAAVYDFGEDGARPFLVMELVEGETLAAR
                     LAREGPLDPAEVTRIGAAVAEALAAAHEAGVVHRDVKPGNVMLATSGQVKVLDFGIAA
                     TPDATTLTASGSVLGTAPYLAPERVRGDRATPASDLYALGVMLYEMLAGEAPFARETP
                     AATALAHLHDEPPPLRARRPDTPPALAALVAALLEKDPAARPASGAVVAASLGAAPPT
                     RPIAVPEPATAPIPLGPGRAPSGRRSRLPLVALALLLALVAGVIAFQGTGDRLEPVPP
                     ASPRPTTTAPALVRVPDLRGPADEDRVRSVLAAAGLDFGGIRVVDEGPAGIVGSEPSA
                     GTLVPKGTDVVVLVNLPGRDGGKGKDKGGDQARGNEEGQD"
     gene            complement(4864..6342)
                     /locus_tag="VEO00_06335"
     CDS             complement(4864..6342)
                     /locus_tag="VEO00_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYU57652.1"
                     /translation="MDRQIRRLGAGLLVLFLALFAQVNYIQVIRADDLANNPGNGKRL
                     LIAEYDVQRGAILARDKRTVLARSRPTEGEFRFLRVYPHGPRYGQITGFYSIASGRSD
                     LELTFNDYLSGRAGELAPQRLVDEILGRPKRGGTVVTTIDARLQEAAEQALGNRRGAV
                     AAIDPRTGEVLALVSNPSFDPNPLATHDIDAARQAYLDLEGQSPTPLRSNAYQQLFFP
                     GSTFKLVTAAAALENGIRPDTSFPNPPTLDLPQTDRTLQNFGGGHCLGGAARITLAQA
                     FQISCNVTFAEIGLQLGADRLLAQAGAFGWNGSLPFDAEFSEGLFPPESVGPDRLPAV
                     AFSAIGQQDVRANPMQMALVAGSIANGGVMMRPRLVREILDPSGRVIRSFGPDEIGRP
                     VSPQTAAELTAMMVSVVEGGTGSAAAIPGVRVAGKTGTAQAAEGQAPHAWFVSFAPAD
                     SPQIAVAVVVLDGGDLGSEATGGAVAAPIAKQVIEAALGGGA"
     gene            complement(6342..>6887)
                     /locus_tag="VEO00_06340"
     CDS             complement(6342..>6887)
                     /locus_tag="VEO00_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009232666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HYU57653.1"
                     /translation="LFVVGAYFGYLAFAHVQDRVFIWLHALDADLISREGASQLAQSM
                     FAMGTGGISGVGLGQGSPGLIPFAPTDFIFAAIGEELGLFGTVALLMLYLAMVSHGFK
                     AAVEADDGFGKLLAVGLSTSLALQTFVIVGGVTRLIPLTGITLPFVSYGGSSLVANFA
                     VIALLVRTSAASRVREREGAR"
BASE COUNT          898 a         2495 c         2550 g          944 t
ORIGIN      
        1 gtgaggggct tcggcgcggt tcctctcatg gcgacgatcc ggctgtgccg gctcgtcgcg
       61 cgcggcggcg ctcgtcggtg cggcgctccg cggccagacg ctccaggtcc acggcggagc
      121 ggtccacgtg gcgcatggcc gccgcgatgc cgcgatgccc cgccacctcg ccgatcatcc
      181 ggtgcagctc gtgcgcgacg ctccggtagg cggggtggcc ctgcggcgac gagcgcagct
      241 cgatcagatg catcgcctct ctggcgttca tctgcatgac gaagcggata cggtgcgcca
      301 tcggcaccgg gtactgggcc gccggcccga gccccgcctc ccggatccgc gtggacaggt
      361 cggcgcactc ggccatcacg ccgtcccact cggcgcgcgc tccggcctcg tccacctcgg
      421 gcggggtctc gtacccgtgg cgcggcgtga gctcctgcca ctggatcgtc agcggccggt
      481 ggcgctgcag gtcgcggaac gcgccgtagt cgcacagcac ctcgaaccgg tacccggtgc
      541 gctcgaaggc ccgccccgga cggtggcggc ggttctcgcg cgtccccacc gcggcggaga
      601 ggatcgcgcc acgctcgccc gcggagaggg cgcggacgcg ctcgagcagg acgtcgtcgg
      661 gcaggtgggt ctcctcgtac aggcatgcgg cggtgacctt cagctcgccg tcggggtcga
      721 agtcggtcag cgtgacgagc gggcgcggcg ggtcgggcac gtccgccagg aacgtctcgg
      781 cgcgctcggc cgtcgccttc cggcgttcct ccagatagcg cgaccaggcc atgccgcgac
      841 cctcgaggtc cacgcggcgc aggaaggcgg ggatcacctt gcgcagctcc acgagcatca
      901 ggtcgccgta ctcgcggacc tcgcgcagcg ggtgagcgcg catccgcagc aggaggtgct
      961 cgtaggactg gccggtggcg aagatgccca cgttcgagac ggtggcggcg gggagcagcc
     1021 cgcgcagcgt gtcgcaggcc ttcgcccgga tcgtcatccg gtagacgaag tccgagtcct
     1081 cagcctggcg tgggaagcgt tcgcggaaga aggcctgcaa cggtccgagc cagcgggcgt
     1141 aggtctcgaa cgcccggtcg agcgcggcgc ggtacggcgc ggcgagcgcc gtgtcctcga
     1201 gctccgacgg ggtgcggaat cgccagcgcc cggccgggcg gtcgtcgtag gggacgtagc
     1261 gcgtggactg ctcgaggtac gcggcgaggc gcccgcgctc gaggaccctg ccgagcaact
     1321 gcgacgcctg ctcgcaggcc aggtgaacgc cgccgagctg ggccacggag tcgtcgccgt
     1381 actcgaagaa gatccgcccg tacagctcct cggcgtggcg ttcgccgacc gccgcctcgg
     1441 ccgcgccggc ggaccgctcg gcaggctcgg cgaactcgtc caggaacagc cgccggagcg
     1501 acttcggcga ccgcgagtag cgggcaaaga gcgccccctt cacggtctcg gacaggttgg
     1561 ccagcgcgaa caccggcccg tcgaggttcg tcacgtaggg cgccagcagg cgccgttcct
     1621 cgtcgctgaa ctcctcgtgg acgaactcga gcatcgccgg cccctccgaa agcggggtca
     1681 tgctacatgc ggggggtgac gcgtcaggcc tgcagcgcga tgtcgaccga ggtcccggga
     1741 gggacctgcg tgccgtcgtc gggttcctgg gcgcagaccg tgcccgagcc gttcggggag
     1801 atgcaggtcg caccgccggt ggtcgtgtcg ccgagctgca gcccgccgtc cttgagggcc
     1861 tgcttggcct cggtctcggt catcccggtc aggtcgggga cctcgatgtt ctgcggctgc
     1921 accgtcacgt cgacctcggt gtccggattc acctggacgt tcgggtcggg gtcctgggca
     1981 cagatcgtcc ccggcccgcc gtcggaggtg caggtcccct cctcgacggt gccgagccga
     2041 agccccgcag tctccagcac ctgctgcgcg tcgtccggcg acagtccggt cagcgcgggg
     2101 acgtccacgg gctcctggcc ggcggagagc gtgatgtcga cggcgcttcc ctgcggcacg
     2161 ttctcgccgg cccctgggtc ctggcggaag acgtggtcct cctcgacgtc cgggctgaac
     2221 gcccggtcaa ccgagccgag cgtcaggtcg ttgtcctcga gcgtctggcg ggcctgatcc
     2281 tccgtcttgc cggtgagatc cgggacctcc acctccacag gcccgttcga gacgaccagg
     2341 tcgacggtcg cgccctcgtc ggccgtgtcg tccgccgccg gatcctgctc gatgaccacg
     2401 cccgccggga tgtccgggtt ggcgcggcgc tccgagacgt tcgccttcag gccctcctgc
     2461 cggagcagca accggcaggc gtcgagccgg cggttcacgc agttcgggac cgtgacctgc
     2521 gccacatccc cgtcccctat gatctggcgg gcgagcagga agaacagcac gccgagcgtc
     2581 gccagcagca ggatcacggc gatgacgaac gccgccgtcc ggccggcgct gcggcccgcg
     2641 ggcccggcgc cggcttcggt ggcggggagc acgagcgtct cccgcgcctc gcgagcgatg
     2701 acctcggtcg gctcgggcgg gagcagcgga gtggcctgga ccgactggcc ggccaggaag
     2761 cgctgcaggt ccgcgagcat ctccgccgcg ctctgatagc ggttcgccgg attcttcgcc
     2821 agcgccttca gcacgatcga ctcgagcggc tggggcacgt cggcgttcag ccgcgacggg
     2881 cggatcgcct cctcctggac gtgcttgtag gcgatggcca ccgggttgtc cccgctgaac
     2941 gggacctggc gcgtcagcat ctcgtagagc accaccccca gcgagtagat gtcggagcgg
     3001 gcgtcgaccg actcgccctg ggcctgctcg ggggagatgt aggaggcggt gccgaggacg
     3061 gtggccgtct gcgtcagggc ctcgccgctc gtggcccggg cgatgccgaa gtccatcacc
     3121 ttcacctcgc ccgtcggcgt gagcatgatg ttccccggct tcacgtcgcg gtggacgatg
     3181 ccgcgctcgt gcgcgaaggc cagcgcctgg cagaccgccg ccgcgatctc ggcggcgcgc
     3241 tcggggagga gcggtccctc ctcgcggatg acctcgcgga gcgtccggcc ctccacgagc
     3301 tccatgacga tgtagtgcac gccgcggtcc gagccggtgt cgtagacgtt gacgacgttc
     3361 gggtggttca tcgccgccgc cgactgcgcc tcgcgccgga accgcgcgac gaacgtggcg
     3421 tcgttcgcgt agcggggggc gaggaccttc accgccacgg ggcgccccag cacggtgtct
     3481 accccgcgga acacggtggc catcccgccc tcaccgagcg gcgcgtggac ctcgtagcgg
     3541 ccgccgagca cgcgccgtcg ctctccgctc agtcctggcc ctcctcgttt cctcgggcct
     3601 ggtccccgcc cttgtccttg cccttcccgc cgtcgcgtcc ggggaggttg accaggacga
     3661 cgacgtcggt ccccttcggg accagggttc cggcgctcgg ttcgctgccc acgataccgg
     3721 ccggaccctc gtccacgacg cggatgccgc cgaagtcgag cccggcggcg gccaggacgc
     3781 ttctgacccg gtcctcgtcc gcggggccgc gcaggtccgg gacccgcacg agggcgggag
     3841 cggtcgtggt cggacggggc gatgcgggcg ggaccggctc caggcggtcg ccggtgccct
     3901 ggaaggcgat gaccccggcg acgagggcca gcagcaacgc cagggccacg agcggcaacc
     3961 ggctgcggcg gcccgagggt gcgcgccccg gtcccagcgg gatgggtgct gtcgcgggct
     4021 ccgggaccgc gatcgggcgg gtggggggag cggcgccgag actcgcggcg accacggccc
     4081 ccgaggcggg ccgggccgcg gggtccttct ccaggagcgc cgcgacgagg gcggccaggg
     4141 ccggcggcgt gtccggccgg cgggcgcgca ggggaggggg ctcgtcgtgc aggtgtgcga
     4201 gggccgtggc ggccggggtc tcgcgagcga agggcgcctc gccggccagc atctcgtaga
     4261 gcatcacgcc gagcgcgtag aggtcggaag ccggggtggc gcggtcgccg cgcacgcgct
     4321 cgggcgccag gtacggcgcg gtcccgagca ccgacccgga ggccgtcagc gtcgtcgcgt
     4381 cgggggtggc ggcgatcccg aagtcgagca ccttcacctg gccggacgtg gcgagcatga
     4441 cgttccccgg cttgacgtcg cggtgaacca cgccggcctc gtgggcggcg gccagggcct
     4501 ccgccacggc ggcgccgatc cgggtcacct cggccgggtc cagcggtcct tcccgggcca
     4561 ggcgcgcggc cagcgtctcg ccctcgacca gctccatcac caggaacggc cgggcgccgt
     4621 cctcgccgaa gtcgtagacc gcggcgatgt tcgggtgcgc cagggcggcc gcggctcgcg
     4681 cctcccgccg gaagcgctcg cgtgaggacg gatcgtcgag cagcccctcg tgcagcagct
     4741 tgacggccac cggccggccg agctcgatgt cctcgccgcg ccagacggcg cccatgccgc
     4801 ccgatgcgag ccgctcgctc agccggtagc ggccccgcgc cagcgactcg gtggccggcg
     4861 ggctcacgcg ccgcctccca gcgccgcctc gatcacctgc ttggcgatcg gggcggccac
     4921 agcgccgccg gtcgcctcgc tgcccagatc gccgccgtcg aggaccacga ccgccacggc
     4981 tatctgcggg gagtcggccg gcgcgaacga cacgaaccag gcgtgcggcg cctggccctc
     5041 ggcagcctgc gcggtgccgg tcttgccggc cacgcggacg ccggggatcg ccgccgccga
     5101 cccggtgccg ccctcgacga cgctgaccat catcgcggtc agctcggcgg cggtctgcgg
     5161 cgagaccggc cggccgatct cgtccgggcc gaaggagcgg atcacgcgcc cggaggggtc
     5221 gaggatctcg cgcaccagcc gcggccgcat catcaccccg ccgttcgcga tcgagcccgc
     5281 gacgagcgcc atctgcatcg ggttggcccg tacgtcctgt tggccgatgg ccgagaacgc
     5341 caccgccggc aggcggtccg gcccgacgct ttccggtggg aacaggcctt cgctgaactc
     5401 cgcgtcgaac gggaggctgc cgttccagcc gaaggccccg gcctgggcga gcaggcggtc
     5461 cgccccgagt tgcagcccga tctcggcgaa cgtcacgttg caggagatct ggaacgcctg
     5521 cgccagcgtg atccgcgccg ccccgccgag gcagtgcccc ccgccgaagt tctgcagcgt
     5581 gcggtcggtc tgggggaggt cgagcgtcgg cgggttcggg aaggaggtgt ccggccggat
     5641 gccgttctcg agcgccgccg cggcggtgac gagcttgaac gttgagcccg ggaagaacag
     5701 ctgctggtag gcgttcgagc gcagcggggt tgggctctgc ccctcgagat ccaggtacgc
     5761 ctgccgcgcg gcgtcgatgt cgtgcgtggc cagcgggttt ggatcgaacg acgggttcga
     5821 gacgagcgcc agcacctcgc ccgtccgcgg gtcgatggcg gccaccgcgc ctcggcggtt
     5881 gccgagcgcc tgctccgccg cttcctgcag ccgagcgtcg atcgtcgtca ccaccgtgcc
     5941 gccgcgcttc ggccggccga ggatctcgtc caccagccgc tgtggggcca gttcgcccgc
     6001 gcggcccgac aggtagtcgt tgaaggtgag ctcgaggtcg ctgcgcccgg aggcgatcga
     6061 gtagaacccg gtgatctgcc cgtagcgcgg gccgtgggga tagacgcgca ggaagcggaa
     6121 ctcgccctcg gtcgggcggc tgcgcgccag gaccgttcgc ttgtcgcgcg ccaggatcgc
     6181 gccacgctgc acgtcgtact ccgcgatcag gaggcgcttg ccgttccccg ggttgttcgc
     6241 cagatcgtcg gcccggatca cctggatgta gttgacctgc gcgaacagcg ccaggaacag
     6301 gacgagcagg cccgccccca gccgccggat ctgccgatcc atcacctcgc cccctcccgc
     6361 tcccggaccc ggctggcggc cgaggtgcgc acgaggagcg cgatcacggc gaagttcgcc
     6421 accaggctcg agccgccgta ggagacgaac ggcagcgtga tcccggtgag cgggatcagg
     6481 cgggtcacgc ccccgacgat gacgaacgtc tgcagcgcca ggctggtgga caggccgacc
     6541 gccaggagct tgccgaaccc gtcgtcggct tccaccgccg ccttgaagcc gtggctgacc
     6601 atcgccaggt acagcatcag cagcgcgacg gtgccgaaca ggcccagctc ctcgccgatc
     6661 gcggcgaaga tgaagtcggt gggggcgaac gggatcagcc cggggctccc ctggccgagc
     6721 ccgacgccgg agataccgcc ggtccccatc gcgaacatcg actgcgccag ctggctggcg
     6781 ccctcccgcg agatcaggtc ggcgtccagg gcgtgcagcc agatgaacac ccggtcctgg
     6841 acgtgggcga acgccaggta gccgaagtac gccccgacga cgaagag
//
LOCUS       DASPWO010000280         3095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_298438, whole genome shotgun sequence.
ACCESSION   DASPWO010000280 DASPWO010000000
VERSION     DASPWO010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298438"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1810
                     /gene="dnaK"
                     /locus_tag="VEO00_06345"
     CDS             <1..1810
                     /gene="dnaK"
                     /locus_tag="VEO00_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HYU57654.1"
                     /translation="PNAEGSRITPSVVAFSKSGEILVGEVAKRQAITNPDRTIRSVKR
                     QMGSDWSVEIDGKRWTPQEVSAQILGKLKRDAEAYLGGPVAEAVITVPAYFDDAQRQA
                     TKEAGQIAGLEVLRIINEPTAASLAYGLDREHEEQTILVFDLGGGTFDVSLLEIGEGV
                     FMVKATNGNTQLGGDDWDQRIIDWMVKEFKASHGVDLSKDRMAMQRLKETAEKAKIEL
                     SQVQETTINLPFITATKSDDGTMSPLHLELKLTRSEFERMTEDLVQAAAGPFQQAVKD
                     WGKDLSAIHHVILVGGATRMPMIQNLIRELTGGKEPHKGVNPDEVVAVGAALQAGVLR
                     GEVKDVLLLDVTPLSLGIETKGGIFTKLIERNTTIPTKKSEIFTTAEDGQTQVEVHVL
                     QGETETVFSPGSKSLGKFQLMDIPPAPRGMPQIEVTFDIDANGIVNVSAKDLGTGKEQ
                     AMTITGGTALPKDEMDRMVKEAEAFAEEDRKRREAAEARNTADNLTYQVDRALSEYGD
                     KIPDAERQGVVTANDELKEALKGDDTDRITAATDRLMSEFQKVGQAVYQAQQAAGAGA
                     GGGGGASGPASETGDPGEGGGEDVVEGEIVDEGGAS"
     gene            1841..2296
                     /locus_tag="VEO00_06350"
     CDS             1841..2296
                     /locus_tag="VEO00_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYU57655.1"
                     /translation="MADLVRRWDPFRDLLGIQEELNRLFGRTLRYGDEVEGESPGAWI
                     PAIDIRETADGYVVETDLPGIDPKDVDITVEDNTLTIKGERRVERETEGEGFHRSERR
                     YGSFVRSITLPATAKQDEIEASFDRGVLRLSIPKAEQAKPRKIEVKAQG"
     gene            2293..2892
                     /locus_tag="VEO00_06355"
     CDS             2293..2892
                     /locus_tag="VEO00_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HYU57656.1"
                     /translation="MKDDEGREAREREVPQGPRVRVTDKRRVGQEGEPAEPAEPGALA
                     PSEASLPDEVGEARARAAEYLDHLQRLKAEFENYRKRVVKEQTRALEYAAEGVVRRLL
                     EVVDEFQLALMAAGAKPDFDRFLRGVELVYAKLSDILHEEGLAPIEARGKPFDPELHE
                     ALMQTDEGEGEAYVADVLRPGYTLKGRVLRPAGVRVARR"
     gene            2924..>3095
                     /locus_tag="VEO00_06360"
     CDS             2924..>3095
                     /locus_tag="VEO00_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HYU57657.1"
                     /translation="MNGNVSRDWFEKDYYAVLGVPRNASAADIKKAYRKLAQQLHPDA
                     NPGNREAEERFKE"
BASE COUNT          574 a          974 c         1173 g          374 t
ORIGIN      
        1 cccgaacgca gaggggtccc gcatcacgcc gtccgtcgtc gcgttctcca agagcgggga
       61 gatcctcgtc ggcgaggtgg cgaagcggca ggccatcacc aaccccgacc ggacgatccg
      121 gtcggtcaag cgacagatgg ggtccgattg gtccgtcgag atcgacggca agcggtggac
      181 cccccaggag gtctcggcgc agatcctcgg caagctgaag cgcgacgccg aggcgtacct
      241 cggcgggccg gtggccgagg cggtgatcac cgtgcccgcg tacttcgacg acgcccagcg
      301 acaggcgacc aaggaggccg gccagatcgc gggcctcgag gtgctgcgca tcatcaacga
      361 gccgacggcc gcgtcactcg cctacggcct ggaccgggag cacgaggagc agaccatcct
      421 ggtcttcgac ctcggcggcg gcacgttcga cgtctcgctg ctcgagatcg gcgagggcgt
      481 gttcatggtc aaggcgacca acgggaacac gcagctcggg ggcgacgact gggaccagcg
      541 gatcatcgac tggatggtca aggagttcaa ggcgagccac ggcgtggacc tctccaagga
      601 ccgcatggcc atgcagcggc tgaaggagac ggccgagaag gccaagatcg agctgtccca
      661 ggtgcaggag acgacgatca acctgccgtt catcacggca acgaagtccg acgacggcac
      721 catgtcgccg ctgcacctcg agctgaagtt gacccgctcg gagttcgagc ggatgaccga
      781 ggacctggtg caggccgcgg ccggcccgtt ccagcaggcc gtgaaggact gggggaagga
      841 tctctcggcg atccaccacg tgatcctggt cggtggggcg acccgcatgc cgatgatcca
      901 gaacctgatc cgtgagctga ccgggggcaa ggagccgcac aagggcgtca accccgacga
      961 ggtggtggcg gtcggcgcgg ccctgcaggc cggcgtgctc cgcggcgagg tgaaggacgt
     1021 cctgctcctc gacgtcacgc cgctgtcgct cggcatcgag acgaagggcg gcatcttcac
     1081 caagctcatc gagcgcaaca ccacgatccc gacgaagaag tcggagatct tcaccacggc
     1141 ggaggacggc cagacccagg tcgaggtgca tgtcctgcag ggcgagaccg agaccgtgtt
     1201 ctcgccgggg tcgaagagcc tcggcaagtt ccagctgatg gacatcccgc cggcgccgcg
     1261 cggcatgccg cagatcgagg tgacgttcga catcgacgcg aacggcatcg tgaacgtgtc
     1321 ggcgaaggac ctcggcacgg gcaaggagca ggccatgacc atcaccggcg gcaccgcgct
     1381 gcccaaggac gagatggacc ggatggtcaa ggaggccgag gcgttcgcgg aagaggaccg
     1441 caagcggcgc gaggcggccg aggcccgcaa cacggcggac aacctgacgt accaggtcga
     1501 ccgggcgctc tcggagtacg gggacaagat ccccgacgcc gagcggcagg gggtcgtcac
     1561 cgcgaacgac gagctgaagg aagccctgaa gggcgacgac accgaccgga tcacggcggc
     1621 caccgaccgg ctgatgagcg agttccagaa ggtcgggcag gccgtgtatc aggcccagca
     1681 ggctgcgggc gccggcgccg gcgggggcgg aggcgcttcc ggcccggcgt cggagaccgg
     1741 cgacccgggc gagggcggcg gtgaggatgt cgtcgagggc gagatcgtgg acgagggcgg
     1801 cgcctcctga cgcgcttgag gaacgaaagg agtgagtgcc atggctgatc tggtcaggcg
     1861 atgggatccg ttcagggacc tcctgggcat ccaggaggag ctgaaccggc tgttcggccg
     1921 gaccctccgc tacggcgacg aggtcgaggg ggagagcccc ggggcctgga tccccgcgat
     1981 cgacatccgc gagaccgcgg acgggtacgt ggtggagacc gatcttccgg ggatcgaccc
     2041 gaaggacgtc gacatcacgg tggaggacaa cacgctgacg atcaagggcg agcgccgcgt
     2101 cgagcgggag accgaaggcg agggattcca ccgctccgag cggcggtacg gctccttcgt
     2161 ccgcagcatc accctgccgg cgaccgcgaa gcaggacgag atcgaggcct cgttcgaccg
     2221 cggcgtgctg cgcctgtcga tcccgaaggc ggagcaggcc aagccgcgca agatcgaggt
     2281 caaggcgcag ggatgaagga cgacgagggc cgcgaggccc gtgagcggga agtgccgcag
     2341 gggccccggg tgcgggtgac ggacaagcgg cgagttggcc aggagggcga gcccgcggag
     2401 cccgccgagc ccggggccct tgccccgtcg gaggcgtccc tgccggacga ggtcggcgag
     2461 gcgcgggctc gcgccgccga gtacctcgac catctgcagc ggttgaaggc ggagttcgag
     2521 aactaccgca agcgcgtggt gaaggagcag acgcgcgcgc tggagtacgc cgccgaaggg
     2581 gtggtgcgcc ggctgctcga ggttgtcgac gagttccagc tggcgctgat ggcggccggg
     2641 gcgaagccgg acttcgatcg gttcctgcgc ggggtcgagc tcgtctacgc caagctgtcg
     2701 gacatcctgc acgaggaggg gcttgcgccg atcgaggcga ggggcaagcc gttcgacccc
     2761 gagctgcacg aggccctgat gcagacggac gagggcgagg gcgaggccta cgtggccgac
     2821 gtcctgcggc ccggttacac gctgaaggga cgggtcctgc ggccggccgg cgttcgcgtg
     2881 gcgcgccggt gaccctgccg gccgtcgcga aggacgggag gccgtgaacg gtaacgtcag
     2941 cagggactgg ttcgagaagg actactacgc ggtccttggc gtgccgcgca acgcctcggc
     3001 ggccgacatc aagaaggcct accgcaagct ggcccagcag ctccaccccg acgccaaccc
     3061 cgggaacagg gaggccgagg agcggttcaa ggagg
//
LOCUS       DASPWO010000281         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_464530, whole genome shotgun sequence.
ACCESSION   DASPWO010000281 DASPWO010000000
VERSION     DASPWO010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464530"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VEO00_06365"
     CDS             <1..1153
                     /locus_tag="VEO00_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HYU57658.1"
                     /translation="RGSYLPSVLNIVQLLGWTAFELWAMALVADRVGRDVLGIGGYGL
                     WLAIVAVVCTLLALGGPLVVVRRWMERFGAWVVAAVALWITIRLLAETDLGAAWSAPA
                     AGGLAFMVGLDLVIVMPISWLPLVADYNRFARPGESSARGTFWGYLVGNVWFYALGAL
                     LVLGAGLSDPSPAGVATAIASLAGGAVVLVALLVGETDQAFANIYSTAVSAQNLAPRL
                     PQRPAILAVGTGGAAIAAWIGADAAGALGNYELFLFGLGSVFVPLYGVLLADYFVLRN
                     HDHAAGELFAPGGRYRFQGGFNVRAFVPWVLGFLVYQWAVPTGPDWWVSAATTFYDRW
                     LQLPFPLFGSRLGASLPAFAVAFIAHLALRPRAVARATRTPPAGRATRS"
     gene            complement(1071..1412)
                     /locus_tag="VEO00_06370"
     CDS             complement(1071..1412)
                     /locus_tag="VEO00_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57659.1"
                     /translation="MEARDLRSFVHFSDDAPVRETVFETERLWAQVICVGRNQSYGPV
                     ADPAADAIVEIVAGEAVVLADRQRKRVKQWETLLIPAGAELSITNASPDPLVVFVLLA
                     PPPAAEAPGGR"
     gene            1527..>2994
                     /locus_tag="VEO00_06375"
     CDS             1527..>2994
                     /locus_tag="VEO00_06375"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HYU57660.1"
                     /translation="MPTFEYSTKAPAEVEADLLIVPVYEGPEAGPGVKEVEKALGVSV
                     LSTLTDNTLKGKVHEFLILPAYGKLGAKTVLVVGLGPRREVSADAIRRAAGYAAKWAS
                     KYGSVASTLPQAGAARIGPAEAVQAFVEGFLLGSYRFVQYKSKPDDERRLDRVLVLGA
                     TRWDARAARTAIERGQVYAEAVAWARDLVNTPAGDATPAFLAEEARTMAREAGLTFKA
                     WTKAELEKGGFGGILGVGQGSANEPRLIELRYRGGGRETVAITGKGITFDSGGLSIKD
                     AVGMEWMKADMGGAAAAIAAMRAIARLKPKINVIAAIPSAENLPSGTALKPGDVLAHR
                     GGKTSEVLNTDAEGRLVLADALAYLAEQKPAAILDAATLTGACMIALGEDVWGVMGND
                     RRLVREVLDAGDQAGEPGWELPLWRRYKRKIESPVADVKNIGDRYGGAITAALFLAEF
                     VGDVPWAHLDVAGTAFQEKAGDYWPKGATGSPARTFIRW"
BASE COUNT          381 a         1041 c         1130 g          442 t
ORIGIN      
        1 ccgcggctcg tacctgccgt cggtgctgaa catcgtgcag ctcctcggct ggacggcctt
       61 cgagctgtgg gcgatggcgc tcgtcgccga ccgtgtcggg cgcgacgtgc tggggatcgg
      121 cggctacggg ctgtggctcg cgatcgtggc ggtggtgtgc acgctgctcg cgctcggcgg
      181 gccgctcgtc gtcgtccgtc gctggatgga acggttcggc gcgtgggtgg tggccgccgt
      241 cgcgctgtgg atcacgatcc ggctgctcgc cgagacggac ctcggcgcgg cctggtcggc
      301 gcccgccgcg ggcggcctgg cgttcatggt cgggctcgat ctggtgatcg tgatgccgat
      361 ctcctggctg ccgctggtgg ccgattacaa ccggttcgcg cgccccggcg agtcctccgc
      421 ccgcggcacg ttctggggct acctggtcgg gaacgtctgg ttctacgcgc tcggcgcgct
      481 gctcgtcctc ggcgccggcc tgtccgaccc gtctccggcg ggggtcgcca cggcgatcgc
      541 ctccctcgcc gggggcgcgg tcgtcctggt ggcgctcctg gtcggcgaga ccgaccaggc
      601 cttcgccaac atctactcca cggcggtctc ggcccagaac ctggcgccgc gcctgccgca
      661 gcgcccagcg atcctggcgg tcgggacggg cggcgccgcg atcgcggcgt ggatcggcgc
      721 ggacgccgcc ggcgcgctgg gcaactacga gctgttcctg ttcgggctgg ggtcggtgtt
      781 cgtcccgctg tacggcgtgt tgctcgccga ctacttcgtg ctgcgcaacc acgaccatgc
      841 ggccggcgag ctgttcgcgc cgggcggccg ctaccgcttc cagggcgggt tcaacgtgcg
      901 ggcgttcgtg ccgtgggtgc tcgggttcct ggtgtaccag tgggcggtgc cgaccggccc
      961 cgactggtgg gtctcggccg ccacgacgtt ctacgaccgc tggttgcagc taccgttccc
     1021 gctgttcggg tcgcgcctcg gggccagcct gccggccttc gccgtcgcct tcatcgccca
     1081 cctggcgctt cggccgcggg cggtggcgcg agcaacacga acaccaccag cgggtcgggc
     1141 gacgcgttcg tgatcgacag ctcggcgccg gcggggatca gaagcgtctc ccactgcttg
     1201 acgcgcttgc gctggcgatc ggccaggacg acggcctccc cggcgacgat ctccacgatc
     1261 gcgtcggccg cggggtcggc gaccggtccg taggactggt tgcgcccgac gcagatcacc
     1321 tgcgcccaca ggcgctcggt ctcgaacacg gtctcccgaa cgggagcgtc gtccgagaag
     1381 tggacgaagc tccgcaggtc cctggcctcc atgcggccgc caagcctacg ggcggcgagc
     1441 cgaaacgact agactcaccc gagtttcgcg ccggcccgcc gccatcgcag ccgcgccccg
     1501 gaaccgaaat ctgaccagga ggcccagtgc ccacgttcga gtactccacg aaggcgccgg
     1561 ccgaggtcga ggccgacctc ctcatcgtgc cggtctacga ggggccagag gccggccccg
     1621 gcgtgaagga ggtcgagaag gcgctcggcg tctcggtcct gtcgaccctc accgacaaca
     1681 ccctgaaggg caaggtgcac gagttcctga tcctccccgc ctacggcaag ctcggtgcga
     1741 agacggtgct cgtcgtgggg ctcggccccc gcagggaggt gagcgccgac gcgatccgcc
     1801 gcgccgccgg gtacgccgcc aagtgggcct cgaagtacgg ctcggtggcg tccacgctgc
     1861 cgcaggccgg ggccgcccgg atcggccccg ccgaggccgt ccaggccttc gtggagggct
     1921 tcctgctcgg ctcgtaccgc ttcgtgcagt acaagtcgaa gccggacgac gagcggcgtc
     1981 tcgaccgggt cctggtgctc ggcgcgaccc ggtgggacgc gagggccgcc cggaccgcca
     2041 tcgagcgcgg ccaggtctac gcggaggcgg tggcctgggc gcgggatctg gtgaacacgc
     2101 cggccggtga cgccacgccg gcattcctgg cagaggaggc ccggacgatg gcccgcgagg
     2161 cgggcctgac gttcaaggcc tggacgaagg cggagcttga gaagggcggg ttcggcggga
     2221 tcctcggcgt cggccagggg agcgccaacg agccgcggct gatcgagctc cggtaccggg
     2281 gcggcgggcg ggagaccgtg gcgatcaccg gcaagggcat cacgttcgac tccggtgggc
     2341 tgtcgatcaa ggacgccgtc gggatggagt ggatgaaggc cgacatgggc ggggccgccg
     2401 ccgcgatcgc ggcgatgcgc gcaatcgccc gcctgaagcc gaagatcaac gtgatcgcgg
     2461 cgatcccgtc ggccgagaac ctgccgagcg gcaccgcgct gaagcccggc gacgtgctcg
     2521 cccatcgcgg cggcaagacc tcggaggtgc tgaacaccga cgccgagggc cgcctggtgc
     2581 tcgccgacgc cctggcctac ttggccgagc agaagccggc ggcgatcctc gacgcggcca
     2641 cgctgaccgg cgcctgcatg atcgcgctgg gggaggacgt ctggggcgtg atgggcaacg
     2701 accggcggct ggtccgcgag gtgctcgacg ccggcgatca ggccggggag ccgggctggg
     2761 agctgccgct ctggcgccgg tacaagcgga agatcgagtc gccggtggcc gacgtgaaga
     2821 acattggcga ccgctacggc ggcgcgatca ccgcggcgct gttcctggcg gagttcgtcg
     2881 gcgacgtccc gtgggcgcac ctcgacgtgg ccggaaccgc gttccaggag aaggccggcg
     2941 actactggcc gaagggtgcc acggggtctc cggcccgcac gttcatccga tggg
//
LOCUS       DASPWO010000282         4602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_721109, whole genome shotgun sequence.
ACCESSION   DASPWO010000282 DASPWO010000000
VERSION     DASPWO010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721109"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VEO00_06380"
     CDS             complement(<1..259)
                     /locus_tag="VEO00_06380"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYU57661.1"
                     /translation="MKLLELRRHAPRDPERDALTDEGRALAERVGRGMDGPYDAVFSS
                     PALRAAETLAWFLSGFGQPLPHNHAVVDALAGDIDDPTAVEA"
     gene            complement(282..605)
                     /locus_tag="VEO00_06385"
     CDS             complement(282..605)
                     /locus_tag="VEO00_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HYU57662.1"
                     /translation="MDVVTAHIWLKKKDGMSVDEFRDHWLNTHAPIARDGYQHLRSYV
                     VSLVTGAPGGAEPLYDGVAELTWDDREGFVADMRSEASKRGNEDLASFTEAFGMAYVE
                     SHTVK"
     gene            complement(629..1939)
                     /locus_tag="VEO00_06390"
     CDS             complement(629..1939)
                     /locus_tag="VEO00_06390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU57663.1"
                     /translation="MAEGTVTGDDAAIPPEPEKLRIREILADPAVRTIIGCVFIVMLG
                     YGIILPILPLFARSFGVSRSATGFLISSFAIARLVFDLVGGPIVDRYGERATAVAGIL
                     FVAVTSVATGLAPNFALAVLFRGIGGAGSAVLFSALFSYLLKVVPSDRMGRTLSVFYG
                     SFNVGVIAGGPIGGFVAQRFGLRAPLHVYAILLLVSGLLFWRYVKDPAPRATGAEPRL
                     TPEEVLAEREMPLLRRTRARMGALLRRPGFIMVVVVNLAYLWMVGGFFDTLVPLFGAE
                     EIGLNPAGIGVVFSVTLAMEFVVLFPAGALADRIGRKPVMVPALAALAASMAVLGFAP
                     GVIVFGAILAVVGLASGFAGVPPAAMLSDVTPEEASGTAIGMFRFAGDLGFVFGPLLG
                     GWAAQTFGFEAAFAVSTLPILVALALVVRTPETLELRRARQAGR"
     gene            complement(1948..2499)
                     /locus_tag="VEO00_06395"
     CDS             complement(1948..2499)
                     /locus_tag="VEO00_06395"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HYU57664.1"
                     /translation="MVRIYTKRGDDGTTGLLYGGRVSKGGPVPEASGSVDEAVSDLGV
                     ARAACEDASLAGELLRLQRELFVAGADLATNPAERAKLEPGVSLVTAAMVERLEELID
                     ATVEAHPLPDVFVVPGSTPLEAALDVARTSVRRAERMAVQLAGVGEHPVNPEVLRYLN
                     RLSDLLFVLGRAHAGGPEPPSRG"
     gene            2720..3601
                     /gene="dapA"
                     /locus_tag="VEO00_06400"
     CDS             2720..3601
                     /gene="dapA"
                     /locus_tag="VEO00_06400"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HYU57665.1"
                     /translation="MSGRFGAVVTAMATPFTEQGDLDLEAARSLARWLLDTGTDALVV
                     HGSTGEAATMTDGEKADLVRLVKEVAGDAPVIAGTGTYDTRHSIHLSEAARDAGADAL
                     LIVCPYYNKPPQRGVVAHYRAIAEAVDLPVLAYNIPGRTAMRIEHGTLLQLAEIPGVV
                     GVKDATADLDGVSRLIAEAPAGFEVYSGDDWATFAMVALGAVGVISVAAHLAGERMGQ
                     MIALAQSGDAGGARKIHESLLPLYRGLFITSNPIPLKAALDLAGHPVGPPRLPLVPAT
                     EDERAAIRKAMEDAGVL"
     gene            3585..>4602
                     /locus_tag="VEO00_06405"
     CDS             3585..>4602
                     /locus_tag="VEO00_06405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HYU57666.1"
                     /translation="MPGSFDGGRGRSKGPAGKGKPGARLVFLGGLGEVGRNMAALEMG
                     GRILVVDAGLSFPEEDMPGVDLVLPDFGWLHERRADVEAVVLTHGHEDHVGALPYLLR
                     EFKLPVFATALTLAMLEGKLDEHGVRKRAKFHEVKPGGQAATGPFRMRFHRMTHSIPD
                     GCAVAVDTPHGTLLHSGDFKLDQTPLDGRVTDLQGLAEEARRGVHVFLSDSTNAEQPG
                     HVPSEREVGPVLVEIFRRAPRLIVTACFASHVHRVQQICDAARAVGRKVAFLGRSMNQ
                     NMAAATATGNLNVPPKDVVPIEAVKDMDPRSVVIVSTGSQGEPMSAVALMAAHENRWI
                     RLERG"
BASE COUNT          691 a         1656 c         1679 g          576 t
ORIGIN      
        1 gcgcctcgac ggccgtcgga tcgtcgatgt cgccggccag ggcgtccaca actgcgtggt
       61 tgtgggggag cggctggccg aagccgctca ggaaccaggc cagcgtctcg gccgcgcgca
      121 gcgccggcga ggagaacacc gcgtcgtagg gcccgtccat ccctcgcccg acccgctcgg
      181 cgagggcgcg tccctcgtcg gtgagcgcgt cgcgctcggg gtcgcgcggc gcgtgccggc
      241 gaagctcgag gagcttcacg tgccggggcg gccgaaccgt ctcacttcac cgtgtgcgac
      301 tcgacgtagg ccatcccgaa cgcctcggtg aagctcgcca ggtcctcgtt cccgcgcttc
      361 gacgcctccg agcgcatgtc ggcgacgaac ccctcgcggt cgtcccaggt cagctcggcc
      421 acgccgtcgt agaggggctc ggcgccgccg ggcgccccgg tgaccagcga cacgacgtaa
      481 ctccgcaggt gctggtagcc gtcgcgcgcg atcggcgcgt gcgtgttcag ccagtggtcg
      541 cggaactcgt ccaccgacat cccgtccttc ttcttcagcc agatgtgcgc ggtgaccacg
      601 tccaccccct gtcagtctcg cgggcatcct acctacctgc ctggcgggcc ctgcgcagct
      661 ccagcgtctc gggggtccgg accacgaggg ccagcgccac caggatcggg agcgtggaca
      721 cggcgaacgc ggcttcgaaa ccgaacgtct gggcggccca cccgccgagc agcggtccga
      781 acacgaaccc caggtcgccg gcgaaccgga acatcccgat ggcggtgccg gatgcctcct
      841 ccggcgtcac gtctgacagc atggcggccg gcgggacgcc ggcgaacccc gaggccagcc
      901 cgaccaccgc caggatcgcg ccgaacacga tcaccccggg agcgaacccg agcacggcca
      961 tcgaggcggc gagggcggcg agcgcgggga ccatcaccgg cttgcggccg atccggtcgg
     1021 ccagggcgcc ggccgggaag aggacgacga actccatggc cagcgtgacc gagaacacca
     1081 cgccgatccc cgccgggttc agcccgatct cctccgcccc gaagagcggg acgagcgtgt
     1141 cgaagaaccc gcccaccatc cacaggtagg cgaggttcac gaccacgacc atgatgaagc
     1201 ccgggcggcg gagcagcgcc cccatccggg cgcgcgtccg ccgcaggagc ggcatctcgc
     1261 gctcggcgag gacctcctcc ggcgtcagtc gcggctcggc gcccgtcgcg cgcggggcgg
     1321 gatccttgac gtagcgccag aacagcaagc ccgagaccag cagcaggatc gcgtagacgt
     1381 ggagcggcgc gcgaagcccg aacctctggg cgacgaaccc gccgatcgga ccgccggcga
     1441 tcacgccgac gttgaaggac ccgtagaaca ccgacagtgt ccggcccatc cggtcgctgg
     1501 gcacgacctt cagcaggtag ctgaagagcg ccgagaacag gacggcggac cccgccccgc
     1561 cgatcccccg gaacagcacc gcgagggcga agttcggggc gagccccgtc gccaccgagg
     1621 tgacggcgac gaacaggatc ccggccactg ccgtggcgcg ttccccgtac cggtcgacga
     1681 tcggcccgcc caccaggtcg aagaccaggc gggcgatggc gaacgaggag atcaggaagc
     1741 cggtggcgct gcggctgacg ccgaacgacc gggcgaagag cggcaggatc gggaggatga
     1801 tcccgtagcc gagcatgacg atgaagacgc acccgatgat cgtgcgcacg gcggggtcgg
     1861 ccaggatctc ccggatgcgg agcttctcgg gctccggggg gatcgcggcg tcgtctccgg
     1921 tgacggtgcc ctcggccatc ggccgggtca gccgcggctc ggcggctcgg gccctccggc
     1981 gtgcgcgcgg ccgagcacga acaggaggtc ggacaggcgg ttcaggtagc gaagcacctc
     2041 cgggttgacg gggtgctcgc cgacgccggc cagctgcacc gccatgcgct ccgcgcggcg
     2101 cacgctcgtc cgggcgacgt ccagcgcggc ctcgagcggc gtcgagcccg gcaccacgaa
     2161 gacgtcgggg agcgggtgcg cctcgacggt ggcgtcgatc agttcctcga gccgctcgac
     2221 catcgccgcg gtgaccaggg agacgcccgg ctcgagcttc gcgcgctcgg cggggttcgt
     2281 cgccaggtcg gcgccggcga cgaagagctc ccgttgcagc cggaggagct ctcccgccag
     2341 cgaggcgtcc tcgcaggcgg cgcgggcgac cccgaggtcg gagaccgcct cgtcgacgct
     2401 gcccgatgcc tccgggaccg gcccgccctt gctgacgcgg ccgccgtaga ggagcccggt
     2461 ggtgccgtcg tcgccgcgct tcgtgtagat ccggaccatg gatgagctcc cgttcccgct
     2521 ccgggctagg atagccgagg cgccggccgc ggacgcggta tgccacgggt tgaacatgtg
     2581 cttcctgtgg ctcttgtgag tcggcggcaa caggtgttac agtcggcgaa ccgccggagc
     2641 gggggcgacc gtcgcaaagg cagggatcct gcccggggcc cggtcgcctc gctcctcgcg
     2701 gtcccgcgaa ggaggagcca tgtccggacg attcggcgcc gtcgtgaccg cgatggccac
     2761 cccgttcaca gagcagggag acctcgacct ggaggccgcc cgctcgctgg cgcggtggct
     2821 gctcgacaca ggcaccgacg ccctggtggt gcacgggtcg accggcgagg cggccacgat
     2881 gaccgacggg gagaaggcgg acctggtccg cctggtgaag gaggtcgccg gagacgcccc
     2941 cgtcatcgcc gggaccggga cctacgacac gaggcactcg atccacctct ccgaggcagc
     3001 gcgcgatgcc ggcgccgacg cgctgctgat cgtctgcccc tactacaaca agccgccaca
     3061 gcgcggggtc gtggcgcact accgcgccat cgccgaggcg gtggacctcc cggtgctcgc
     3121 ctacaacatc cccgggcgca ccgcgatgcg catcgagcac ggaacgctcc tgcagctcgc
     3181 ggagatcccc ggcgtggtcg gcgtgaagga cgccaccgct gacctcgacg gcgtctcgcg
     3241 cctgatcgcg gaggcgccgg ccggcttcga ggtgtactcg ggggacgact gggcgacgtt
     3301 cgccatggtc gcactcgggg cggtcggcgt gatctcggtc gccgcgcacc tcgccggcga
     3361 acggatgggc cagatgatcg ccctggcaca gtcgggcgac gccggcggcg cgcgcaagat
     3421 ccacgagagc ctccttccct tgtaccgggg cctgttcatc accagcaacc cgatcccgct
     3481 gaaggccgcc ctcgacctgg ccggccaccc ggtcgggccg ccacggctgc cgctcgttcc
     3541 ggccaccgag gacgagcgtg ccgcgatccg gaaagcgatg gaggatgccg gggtcctttg
     3601 acggcgggcg tgggcgctcg aaggggccgg cggggaaggg gaagcccggg gcgcggctcg
     3661 tcttcctcgg ggggctcggc gaggtcggcc ggaacatggc cgccctggag atgggcggcc
     3721 ggatcctggt ggtcgacgcc ggcctgtcgt tcccggagga ggacatgccc ggcgtggacc
     3781 tggtcctgcc cgacttcggg tggctccacg agcggcgcgc cgacgtcgag gccgtcgtcc
     3841 tgacccacgg ccacgaggac cacgtcggcg ccctcccgta cctgctgcgc gagttcaagc
     3901 tgccggtgtt cgccaccgcg ctgacgctgg cgatgctcga gggcaagctc gatgagcacg
     3961 gcgtccgcaa gcgcgcgaag ttccacgagg tgaagccggg cgggcaggcc gcaaccggcc
     4021 cgttccggat gcgcttccac cggatgaccc actccatccc ggacggctgc gcggtggccg
     4081 tcgacacccc gcacggcacg ctgctgcact ccggggactt caagctcgac cagaccccac
     4141 tcgacgggcg ggtgaccgac ctgcagggcc tggccgagga ggcgcgccgc ggcgtccacg
     4201 tcttcctgtc ggactcgacc aacgccgagc agccggggca cgtgccctcg gagcgagagg
     4261 tcgggcccgt cctcgtcgag atcttccggc gcgcgccgcg cctgatcgtc acggcctgct
     4321 tcgcgagtca cgtgcaccgc gtgcagcaga tctgcgacgc ggcccgcgcg gtggggcgga
     4381 aggtggcgtt cctggggcgc tcgatgaacc agaacatggc cgccgccacc gcgaccggga
     4441 acctgaacgt cccgccgaag gacgtcgtgc cgatcgaggc ggtgaaggac atggacccgc
     4501 ggtcggtggt gatcgtgtcc accggaagcc agggcgagcc gatgtcggcg gtcgccctga
     4561 tggcggcgca cgagaaccgg tggatccggc tggagcgcgg cg
//
LOCUS       DASPWO010000283         3345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_43243, whole genome shotgun sequence.
ACCESSION   DASPWO010000283 DASPWO010000000
VERSION     DASPWO010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43243"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VEO00_06410"
     CDS             complement(<1..658)
                     /locus_tag="VEO00_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYU57667.1"
                     /translation="MTASELARGEEPVLREPTFVDVLHARRVIEPYLGRTAFHESISL
                     GTMLGLTLYIKYENHQPIGSFKVRGTLNKVLSLSVEERAAGVVTASMGNHGQGVCYAA
                     RLLGVRATVVMPDVANPDKVEAVRNLGAEVIFAGEDFEAANVAARELERDKGFVYFHS
                     GNDPFIIAGHGTIGLEMLEDAPDLDTIVVPIGGGGLMSGISIAARAGKPDIELIGVEA
                     E"
     gene            complement(678..1703)
                     /locus_tag="VEO00_06415"
     CDS             complement(678..1703)
                     /locus_tag="VEO00_06415"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018751424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HYU57668.1"
                     /translation="MSERVAVVGAGSWGTAFATITAEKGIDTMLWARRREIAQEIAGD
                     HSNARYLPDVGLPASLGATDDLEAAVSGAEVVVMAVPSHAYREIFRTLVPFLGPEAPV
                     VSLTKGIEQDTQKRMTEVMAEEAGLGEARVAVLTGPNLAREVMDRMPSATVVACADQA
                     VAARLQDLFMAPFFRVYTNPDVVGCELAGAMKNVLAIAAGIADGLGFGDNSKASWITR
                     GLAELARLGQKLGGNPLTFAGLAGMGDLIATCISKLSRNRHVGEELGKGRSLETIVNE
                     MNMVAEGVKTSRAVVAIAEREGVEVPIAEHVVKVLYEDVTPRDMVMSLMLRSAKAELA
                     GIQGETG"
     gene            complement(1717..2385)
                     /locus_tag="VEO00_06420"
     CDS             complement(1717..2385)
                     /locus_tag="VEO00_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYU57669.1"
                     /translation="MAEPWYALTVGVLRPSLALYFNWRFEGLEQVPRTGPLLVAANHI
                     SELDPLAHGYFLVKAGRRPRFLGKSELWRNALLRPMLRGTRQIPVHRGTGEATPVETA
                     VERLRRGECVVVYPEATLTKNPDYSPMRAKTGIARVAIEASVPVLPVAAWGTHKVLPR
                     HGEQRNLAWARPIMVKAGPPLDLSRYEDDEDRGAYREATDEVMAELSGLVSDLRARYP
                     KRWA"
     gene            2419..3093
                     /gene="cofC"
                     /locus_tag="VEO00_06425"
     CDS             2419..3093
                     /gene="cofC"
                     /locus_tag="VEO00_06425"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HYU57670.1"
                     /translation="MRVVAVPAKAFRLSKSRLAPVLTPAERASLSAAMLEDVLAATAA
                     TTGWERWMVSADPAALALAERLGARAVPEERRSLLAAVRQAEALAAAEDAEALAVVLA
                     DLPLLSTPALAAALAEPAAVVLAPAESDGGTNLLLRRPPGVIRARFGRDSFRRHLEEA
                     GAAGVAARVIRRPELAFDLDGPADLDALARRAARPGGRGTPGPRTRAVCLELDVAGRL
                     RAFAAR"
     gene            3124..3330
                     /locus_tag="VEO00_06430"
     CDS             3124..3330
                     /locus_tag="VEO00_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57671.1"
                     /translation="MAQGTIRDFDEEARTGSLLLDDSTEVAIDPISIEGSGIRTLRVG
                     QRVKFDLAEEAGTKLARNLTIITF"
BASE COUNT          443 a         1260 c         1163 g          479 t
ORIGIN      
        1 gctcggcctc gaccccgatc agctcgatat ccggcttccc cgcccgcgcg gcgatggaaa
       61 tgcccgacat cagcccgccg ccgccgatcg gcacgacgat cgtgtcgaga tcgggcgcgt
      121 cctcgagcat ctcgagcccg atcgtcccgt ggccggcgat gatgaagggg tcgttgccgg
      181 agtggaagta gacgaagccc ttgtcgcgtt ccagctcgcg cgcggcgacg ttcgcggcct
      241 cgaagtcctc gccggcgaag atcacctcgg ccccgaggtt gcgcaccgcc tcgaccttgt
      301 ccgggttcgc cacgtcgggc atcaccaccg ttgcccgaac gccgagcagg cgcgccgcgt
      361 agcagacgcc ctggccgtgg ttgcccatcg aggctgtcac gaccccggcg gcgcgctcct
      421 cgacgctcag cgagaggacc ttgttcaggg tgccgcggac cttgaaggac ccgatcggct
      481 ggtggttctc gtacttgatg tacagggtca gcccgagcat ggtgccgagc gagatcgact
      541 cgtggaacgc cgtgcgtcca aggtagggct cgatcacgcg ccgggcgtgg aggacgtcga
      601 cgaacgtcgg ctcgcgcagg accggctcct caccccgggc gagctcggag gccgtcatcc
      661 ccccggtggc gcgcccatca acccgtctcg ccctggatgc cggccagctc cgccttcgcc
      721 gagcgcagca tcagcgacat gaccatgtcg cgcggcgtga cgtcttcgta caggaccttg
      781 accacgtgct cggcgatcgg gacctcgacg ccctcccgct cggcgatggc aaccaccgcc
      841 cggctcgtct tgaccccctc ggccaccatg ttcatctcgt tgacgatcgt ctccaggctg
      901 cgccccttcc cgagctcctc gccgacgtgg cggttccggg agagcttgct gatgcaggtg
      961 gcgatgaggt cgcccatccc ggccagcccg gcgaacgtca gggggttccc cccgagcttc
     1021 tggccgagcc gggccagctc ggccaggccg cgggtgatcc acgacgcctt ggagttgtcg
     1081 ccgaagccca gcccgtcggc gatgccggcc gcgatggcca ggacgttctt catcgcgccg
     1141 gccagctcgc acccgaccac gtcggggttc gtgtaaacgc ggaagaacgg ggccatgaac
     1201 aggtcctgca gccgggcggc aacggcctgg tcggcgcacg ccaccaccgt cgccgacggc
     1261 atgcggtcca tgacctccct ggcaaggttc gggccggtga gcactgccac gcgcgcctcg
     1321 ccgagcccgg cctcctcggc catcacctcg gtcatccgct tctgggtgtc ctgctcgatc
     1381 cccttggtca gcgagacgac cggcgcctcc gggccgagga acgggacgag cgtccggaag
     1441 atctcgcggt aggcgtgcga ggggacggcc atcaccacca cctccgcccc ggagacggcc
     1501 gcctcgaggt cgtcggtcgc cccgagcgat gccgggagcc cgacgtcggg caggtagcgg
     1561 gcgttcgagt ggtcaccggc gatctcctgg gcgatctccc ggcggcgggc ccagagcatc
     1621 gtgtcgatgc ccttctccgc ggtgatcgtg gcgaacgccg tgccccacga ccccgccccg
     1681 acgaccgcca cccgctcgct catcggaccg ttcacctcac gcccatcgct tcgggtagcg
     1741 cgcccgcagg tcggagacca ggccggagag ttcggccatc acctcgtcgg tggcctcgcg
     1801 gtacgcgccc cggtcttcgt cgtcctcgta ccgggagagg tcgagcggcg gcccggcctt
     1861 gaccatgatc ggccgcgccc acgccaggtt gcgctgctcc ccgtggcgcg gcagcacctt
     1921 gtgggtgccc caggcggcga ccggcagcac cgggaccgac gcctcgatcg ccacgcgggc
     1981 gatccccgtc ttcgcccgca tcgggctgta gtcggggttc ttcgtcagcg tcgcctccgg
     2041 gtagaccacc acgcactccc cgcgccgcag gcgctccacc gccgtctcca cgggcgtcgc
     2101 ctcgccggtc ccccggtgga ccgggatctg gcgcgtcccg cgcagcatcg gccgcagcag
     2161 tgcgttccgc cacagctcgg acttccccag gaaccgtggg cggcggccgg ccttcaccag
     2221 gaagtagccg tgcgcaagcg ggtcgagctc cgagatgtgg ttggccgcca cgaggagggg
     2281 gcccgtccgc ggaacttgct ccagcccctc gaaccgccag ttgaagtaga gcgccaggga
     2341 cggtcgcagg accccaaccg tcagcgcgta ccacggctcg gccacgacca ctcatcgtag
     2401 tggagcgata ctggcgccgt gcgcgtggtc gccgtcccgg cgaaggcctt ccggctctcc
     2461 aagtcgcggc tcgctccggt gctcaccccg gccgagcgcg cgtccctctc ggcggcgatg
     2521 ctcgaggacg tcctggccgc gaccgccgcc accaccggat gggagcgatg gatggtgtcc
     2581 gccgacccgg ccgcgctggc gctcgcggag cgcctcggcg cgcgggcggt ccccgaggag
     2641 cggcgctccc tgctcgccgc cgtccggcag gccgaggcgc tggccgcggc cgaggacgcc
     2701 gaggcgctgg ccgtcgtgct ggccgacctt ccgctcctct cgacgcccgc actggcagcc
     2761 gcgctcgccg agccggccgc ggtcgtgctc gcgcccgcgg agagcgacgg tggaacgaac
     2821 ctgctcctgc gccgcccgcc gggcgtgatc cgggcgcgct tcgggcgcga ctcgttccgg
     2881 cggcatctgg aggaggctgg ggccgccggg gtggccgcac gggtgatccg ccgtcccgag
     2941 ctggctttcg acctggacgg cccggccgat ctggacgccc tggcccggcg tgccgcccgc
     3001 cccggcgggc gaggaacccc gggtccgcgg acgcgcgccg tttgcctgga gctggacgtc
     3061 gcggggagac tacgggcctt cgcggcccgc tgaggagccg cgaggacggc acgggagcgc
     3121 gtgatggcgc aggggacgat cagggacttc gacgaggagg cgcggaccgg cagcctcctg
     3181 ctcgacgaca gtaccgaggt cgcgatcgac ccgatctcca tcgaggggag cgggatccgc
     3241 accctgcgcg tcggccagcg ggtcaagttc gacctcgccg aggaggccgg cacgaaactc
     3301 gcccgcaacc tgaccatcat cacgttctga gggcctcggc ggtcc
//
LOCUS       DASPWO010000284         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_384385, whole genome shotgun sequence.
ACCESSION   DASPWO010000284 DASPWO010000000
VERSION     DASPWO010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384385"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..644
                     /locus_tag="VEO00_06435"
     CDS             183..644
                     /locus_tag="VEO00_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYU57672.1"
                     /translation="MAHPRRRDTKTHPQSRPRRLIAPPPSARPGRRLNLSSPHGLRRS
                     RPAPFGAHVRALPLPLAQQPPTSDVLTSPTRSFLLRKLPHPSRAVLSSAGLVINTITM
                     AVLERTREIGVLRSIGGHARDIRRIFGTEGLVVALAGWVIGIPLGLGLAHA"
     gene            complement(652..996)
                     /locus_tag="VEO00_06440"
     CDS             complement(652..996)
                     /locus_tag="VEO00_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57673.1"
                     /translation="MTATGGYTQDSGDTRLQDAASSLAGGGTGAGRTVSISGGTLSGV
                     GTVDGDLVNEGTVQPGNSPGIFTVTGTYDQKGGTLPIEIAGVEAGTQYDSLSVSGAAT
                     LGGTLEISTDPD"
     gene            complement(993..1304)
                     /locus_tag="VEO00_06445"
     CDS             complement(993..1304)
                     /locus_tag="VEO00_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57674.1"
                     /translation="MDHATPNSIVLDSPGASDGGSNPPAAGTFTNLGTVTKQNGTALT
                     SILIPTDNQGTFTVSAGDTEFSSVTNETAGDVSLADGTTASVDTTFANSGQVTLPEAR
                     R"
     gene            1508..1690
                     /locus_tag="VEO00_06450"
     CDS             1508..1690
                     /locus_tag="VEO00_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57675.1"
                     /translation="MSGLAPPTAPPVSARSVIDEGRSPSVIWSPPAETTIASTLSSAW
                     IERDLGSEYVMVGWGS"
     gene            2211..2384
                     /locus_tag="VEO00_06455"
                     /pseudo
     CDS             2211..2384
                     /locus_tag="VEO00_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2506..2721
                     /locus_tag="VEO00_06460"
                     /pseudo
     CDS             2506..2721
                     /locus_tag="VEO00_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
BASE COUNT          415 a         1167 c         1071 g          467 t
ORIGIN      
        1 gctccggctg aagggcctca tcgagcgcgt tcccggcacg cacacctacc tcatcaccgc
       61 ctacggacgg cggatcgcca cgttcttcac ccggctcgcc gctcgggtgg tcgtgcccgg
      121 tctgaccgca ctggacaagg cggcgcggcc accgcggacc gccccccgtc cctgctcgcg
      181 gcgtggcgca cccacgacgc cgagatacga agactcatcc tcagagccgg cctcgccgcc
      241 tgatcgcacc cccaccgtcc gcccggcctg gccgcaggct caacctcagc tcgcctcacg
      301 ggctgcggcg gtcccgcccg gcacctttcg gggcccacgt ccgcgcccta ccattgccac
      361 tggcacagca gccccccact tcagacgtcc tcactagccc cacgcgctcg ttcctgctcc
      421 gaaagttgcc tcatccgtcg cgagctgtct tgtcaagcgc ggggctagtg atcaacacga
      481 tcaccatggc cgtgctggag cgcacccgcg agatcggcgt cctccgctcg atcggcggcc
      541 acgctcgcga catccggcgc atcttcggca ccgagggcct ggtggtggcg ctggcgggct
      601 gggtgatcgg catcccgctc ggcctcggcc tagcccacgc ttagcccacg cttagtccgg
      661 atccgtcgag atctcgagcg tcccgccgag cgtcgccgcg ccgctcaccg agaggctgtc
      721 gtactgcgtc cccgcctcga cgcccgcgat ctcgatcggc agggtcccgc ccttctggtc
      781 gtaggtcccc gtcaccgtga agatgccggg cgagttcccg ggctggaccg tcccctcgtt
      841 caccaggtcg ccgtccaccg tgccgacccc cgacagcgtc ccgcccgaga tcgagaccgt
      901 acgcccggcc ccggtcccgc ccccggccag cgacgatgcg gcatcctgta accgcgtgtc
      961 ccccgagtcc tgggtgtagc cgccggtcgc ggtcatcgac gcgcttccgg cagggtgacc
     1021 tggccggagt tcgcgaacgt cgtgtccacc gacgcggtgg tcccgtcggc caacgagaca
     1081 tccccggcgg tctcgttcgt cacgctcgag aactcggtgt cgccggctga gaccgtgaac
     1141 gtgccctggt tgtcggtcgg gatcaggatg ctcgtcaggg ccgtcccgtt ctgcttggtg
     1201 accgtgccca ggttcgtgaa cgtcccggcc gccggcgggt tcgagccgcc gtccgaggcg
     1261 cccggcgagt ccaacacgat cgagttcggc gtggcgtggt ccatcgtgaa ctgaccggtg
     1321 ttcgcgaagg cccccggcgg ccacggcgat cgtcccgtcg gtccacgtgg ccgtcccgtc
     1381 catcgcgagg tccccgtcca cggatttctg cgccgcgccc ccgaactgca ggtcggcgcc
     1441 ctgggccacg gtggtggcgc ccggaccggt gagccgcccg ccctcgaacg agaaggacac
     1501 ggtcagggtg agcgggctcg cacccccgac ggcgccgccg gtcagcgcga ggtcggtgat
     1561 cgacgagggc aggtcgccgt cggtcatctg gagcccgccg gccgaaacga cgatcgcctc
     1621 gacgctctcc agcgcctgga tcgaacgcga cctggggagc gagtacgtga tggtcggatg
     1681 gggttcgtga acgggacgca gacgaaatcg gcggcgcccg ggacgcgcga gccgccggtg
     1741 gcgacgtccc gccagttccg ggggtcgttc cggtcgtcgg tggtgccccg gccggtccac
     1801 tcgatcgtct cggtgccgct gctgcaggcg gaagccgcgg ccgggacggt gggatggagc
     1861 cagatccccg cggccacgac ggcgaggagg ccggccgcga agcgccgcgc catgcgggcc
     1921 cgcggccgag ctcgatcgcg gcctccacgg gccgatcgtg ttcccccagt cacccccacc
     1981 ccccagctcc ccgccggcct ggtctcgcgc tgccggcagc cccgctgccg cggtcgaggc
     2041 gcgggacaca taccacccac gcggccgggc tgtcgaggca ggatccgccc cggacggctg
     2101 gccggaccgc cgtatcgtgg gctcgacggg tggagggttg aggataccgc tgggcggatt
     2161 cgagctcgat ggtcccctgg agacggagcg gctgatcctt tcggccgtac gtggaagacg
     2221 acttcgaagc gctgttcgcg atccgctccc gcgaggccgt cacgcgatac ctgtactgga
     2281 gcgcccagac caaggcccag gtccgcgaga tcctcgaaca gaagatcgcg agcgtggcca
     2341 tccgctccga gggcgacgtc ctcgccctcg gcgtggtgcc gaaggggacc gacgtcccgg
     2401 tcggcgacgt gatcttccgg tggaccagcg aggcccaccg gcagggcgag ctcgggtacg
     2461 tcgtccaccc ccgaccacca gggccgcggc tacgcgaccg aggcctctcg ggtgctcctc
     2521 cggatcgcgt tcgacgacct ggggctgcat cgcgtgttcg ggagcgtcga ggcccgcaac
     2581 gcggcgtccg cccgcgtgct cgggaagctc ggcatgcgcc gggaggcgca cttcgtggag
     2641 aacgagttcg tcaagggcga atggcagagc gagctcgtgt acgcgatcct cgcgaacgag
     2701 tggcgggccc gcgccggctg atcccctcgc cgagcggtcg ctcgcgtcgg ctcgctaccg
     2761 gccttcaccc acgcccggat cgacgtgggg ctgctcccgc tctggatcgt ccaggcccac
     2821 gacgcgttgc ccgtgttgcc ggcctgatcg gtcgccgaca cgacgaacat gtggtctccc
     2881 ggcggcaggc ccgcatagct gacccccgac gcgcacggct ggggcgtcgc tccgtcgagc
     2941 gagcacgtca cgctcgtgtt cgactcggtc gagaaggtga aggtcccagt gggatcgagc
     3001 gtgggattcg ccgggcccga gtcgatgatc accgccggcg cgctcgtgtc gaccgaccac
     3061 gtcgccgtct cggggcttga cgagttgccg gccgcgtcgg tggcagtcac cgcgaacgag
//
LOCUS       DASPWO010000285         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_299989, whole genome shotgun sequence.
ACCESSION   DASPWO010000285 DASPWO010000000
VERSION     DASPWO010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299989"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..934
                     /locus_tag="VEO00_06465"
     CDS             50..934
                     /locus_tag="VEO00_06465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU57676.1"
                     /translation="MRFAVVIPTLGRPAVLADTLAGLARCTPPPDEVVVVDGDPAASA
                     RATTEGAAAAGGAIRYLIAPASATGQRNRGMADAEGDVLVFLDDDVLVDPAAFAVLAR
                     AYEDPAVIGATGRVIEPASHRYGGRTSRARRWLFGGGREGTFTRFGYPRYLVHADRPR
                     DVEFMPGCFMSARRSDALAVGFDEHLEGYALAEDEDFSYRLSRRGRIRYLPDAVVHHR
                     KLGFGSQDPRAFGRRVVVNRAYLFRKNFPQTALARAQFGLLVAVQIGHRLLNGDWAGA
                     RGLVDGAAAAWRERRSPR"
     gene            931..2070
                     /locus_tag="VEO00_06470"
     CDS             931..2070
                     /locus_tag="VEO00_06470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYU57677.1"
                     /translation="MTAPAQSPSVRVAFVSSHARLGGAERVLETLVEALGPAWEAGMV
                     ALEEGPFARRLDARGHRVEVVPAGRRGVDVAVAARRLRPVLRRMTPQVVHANGVKAAL
                     VTVLARPGAPVMWMKHDFARDGWQARWIARRCAAVVGVSGAVLETFGGRLGGRAAVIH
                     NGLRLPATDRAAGRAALLAAMGHPEPSAVVALVGRVDPFKGHRELLEIATTLRGSVPG
                     VAFAFVGAEDPAHPGTVAALRRRAAELGVADAVRFLGYRDDAVDLIAGADVLTVPSVP
                     DASGMGREGFPLVAIEALAAGTPVIAYAHGGVPELVGDCGRLVPPGDRRHLLAALVEV
                     LTDAALRERLSLCGRARARDAFSVEHMAGAMAARYRAVAAGGMLP"
     gene            2070..>2393
                     /locus_tag="VEO00_06475"
     CDS             2070..>2393
                     /locus_tag="VEO00_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57678.1"
                     /translation="MRAIDRVVGASPVRTALRAVSLIAGLAVTVAIAGAVTAAAVHYG
                     GAAAPALVLAVAILPLVTLLIFLSPPVGPMAVFVTFAAGAASVPFGIVTLQVVEAATL
                     YVALVV"
BASE COUNT          239 a          890 c          924 g          340 t
ORIGIN      
        1 gcgctgaagc tcctcgggac ccgccgccac ggccgctagc cttcttcccg tgcggttcgc
       61 cgtggtcatc cccacgctcg gccggcccgc ggtgctcgcc gacacgctcg ccggcctggc
      121 ccggtgcacg ccgccccccg acgaggtggt cgtcgtggac ggagacccgg ccgcgtcggc
      181 gcgcgcgacc accgagggcg ccgccgcggc tggcggggcg atccgctacc tcatcgcgcc
      241 ggcaagcgcc accggacagc gcaaccgcgg gatggcggat gccgagggcg acgtcctcgt
      301 cttcctcgac gacgacgtcc tggtcgaccc cgccgcgttc gccgtcctcg cccgggccta
      361 cgaggacccg gcggtgatcg gcgccacggg ccgggtgatc gagccggcca gccaccgcta
      421 cggcggccgg acgtcgcgag cgcgccggtg gctgttcggc ggcgggcgcg agggcacgtt
      481 cacccgcttc gggtatccgc gctacctggt ccacgccgac cggccccgcg acgtcgagtt
      541 catgcccggg tgcttcatga gcgcgcgccg gtccgacgcg ctcgccgtcg gcttcgacga
      601 gcacctcgag ggctacgcgc tggccgagga cgaggacttc tcgtaccgac tttcccggcg
      661 cggacggatc cgctacctgc cggacgcggt cgtccaccac cgcaagctgg ggttcggatc
      721 gcaggacccg cgcgcgttcg gccgccgggt ggtggtcaac cgcgcctacc tcttccggaa
      781 gaacttcccc cagacggccc tggcgcgagc gcagttcggg ctgctcgtcg ccgtccagat
      841 cgggcaccgg ctcctgaacg gcgactgggc cggcgcccgt gggctcgtcg acggcgccgc
      901 cgcggcgtgg cgagagcgac ggtcgccgcg atgacggcgc cggcccagag cccatcggtc
      961 cgggtggcgt tcgtgtcctc gcacgccagg ctcggcggag ccgagcgggt cctggagacg
     1021 ctggtcgagg cgctcgggcc ggcctgggag gcgggaatgg tcgcgctgga ggaggggccc
     1081 ttcgcccggc gcctcgacgc acggggccac cgtgtggagg tcgtcccggc cggacggcga
     1141 ggcgtggacg tcgccgtggc cgcgcgccgg ctgcggcccg tcctgcggcg aatgacgccg
     1201 caggtggtgc acgcgaacgg cgtcaaggcc gcgctggtga ccgtcctggc ccggccgggc
     1261 gcgccggtga tgtggatgaa gcacgatttc gcgcgcgacg ggtggcaggc gcgttggatc
     1321 gctcgtcgct gcgccgcagt cgtcggcgtg agcggagcgg tgctcgagac gttcgggggc
     1381 cgcctcggcg gccgcgccgc ggtgatccac aacgggctgc ggctccccgc gaccgaccgg
     1441 gcggccgggc gggccgccct gctcgcggcg atgggccacc ccgagccgtc cgccgtggtc
     1501 gcgctcgtcg ggcgggtcga cccgttcaag ggccaccgcg aactgctcga gatcgccacg
     1561 acgctgcggg gatcggtgcc gggggtggcg ttcgccttcg tcggggccga ggacccggcg
     1621 caccccggaa cggtggcggc gctgcgccgg cgggcggccg agctcggcgt ggccgacgcg
     1681 gtgcggttcc tcggctaccg ggacgacgcg gtcgatctca tcgccggcgc cgacgtcctg
     1741 acggtgccga gcgtgccgga cgcctccggg atggggcggg agggattccc cctggtcgcg
     1801 atcgaggcgc tcgcggccgg gacgcccgtg atcgcctacg cgcacggggg cgtgcccgag
     1861 ctggtcggcg attgcgggcg gctggtcccg cccggggatc gccggcacct gctcgccgcc
     1921 ctggtcgagg tgctgacaga cgcggccctc cgcgagcgac tttcgctctg tggccgcgct
     1981 cgggcccgcg atgccttctc cgttgagcac atggccggcg cgatggccgc ccggtaccgg
     2041 gcggtcgccg ccggtgggat gcttccgtga tgcgagcgat cgaccgcgtg gtcggggcgt
     2101 ctcccgtccg gacggccctt cgggccgtat ccctgatcgc gggactggcc gtgaccgtcg
     2161 ccatcgcggg cgcggtgacg gcggccgccg tccactatgg gggggcggcg gccccggcgc
     2221 tcgtcctcgc ggttgccatc ctcccgctcg tgaccctgct catcttcctc tccccgcccg
     2281 tcgggcccat ggccgtgttc gtgacgttcg ccgccggtgc cgcctcggtc ccgttcggca
     2341 tcgtgacgct gcaggtcgtc gaggcagcca cgctgtatgt cgcgctcgtg gtg
//
LOCUS       DASPWO010000286        11670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_464969, whole genome shotgun sequence.
ACCESSION   DASPWO010000286 DASPWO010000000
VERSION     DASPWO010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11670
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464969"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VEO00_06480"
     CDS             complement(<1..812)
                     /locus_tag="VEO00_06480"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU57679.1"
                     /translation="MSSPDDQGRTEIRTFLIADVRGYTLFSQERGDEAAAKLAAKFAA
                     ISREEIESRGGILLELRGDEALSVFGSARQALRVAIDLQARFVEETESDPSLPLLVGI
                     GLDVGEAVPVEGGYRGRALNLAARLCGEAGPGDVLASRETVHLAGRIDGLTFTERGEL
                     HLKGIAQPVRPIRVASEHEDAALRLAPLATRPPSGPPRRFAPRARVAVAVGAALALVA
                     AAVTIPVLVSGNGDGGPTRTVTPNSVGLVDMTTGRVTGEVTLDARPGSVAAGA"
     gene            complement(903..1673)
                     /locus_tag="VEO00_06485"
     CDS             complement(903..1673)
                     /locus_tag="VEO00_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU57680.1"
                     /translation="MSDLGLAARQVKYQNKLFWRNPASAFFTFAFPLMFLVIFTTLFG
                     NDTIPVLGRTIRVSTYYVSAVATFSIITATYTNLAISVSFSRDAGILKRTRGTPLPSW
                     AFMSGRIVHAILLALLLVVIVAAFGAAFYDAELPTRTLPAFLIAIVVGAGSFSALGLA
                     MTAAIPNAEASPAVVNASILPLLFLSGIFVPLRDTTAWYVTVAKAFPIYHFAQAMQSA
                     YFAPTGNGFAWSDVGIVGAWGVAGVLLATRFFSWEPRK"
     gene            complement(1735..2670)
                     /locus_tag="VEO00_06490"
     CDS             complement(1735..2670)
                     /locus_tag="VEO00_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU57681.1"
                     /translation="MRSSDGGPAISVRGLRKAYGEVEAVRGIDLTVRAGEVFALLGPN
                     GAGKTTTVEILEGFRPRDGGDVDVLGFDPAAGPRHLKERIGIVLQSTGIEPYLTVAET
                     IEMYRGYYPHPRPVDEIVEVVGLSEKRDGRVSKLSGGQQRRLDVAIALAGDPVLLFLD
                     EPTTGFDPGARRNAWDMIRNLRALGKTVLLTTHYMDEAQALADDVAIINRGEIVARGH
                     PTTLGGRDTADTRIRFRLPEADVGELPDPLRSAASDHEGDVVLTTPEPTRLLHELTGW
                     AIGRGIELANLHVTKPSLEDVYLEITGGDAGPEEG"
     gene            complement(2693..3070)
                     /locus_tag="VEO00_06495"
     CDS             complement(2693..3070)
                     /locus_tag="VEO00_06495"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HYU57682.1"
                     /translation="MTREGERRFTVEEANAELEELRTALARIREARQVILRSGERIRS
                     AAPSDGGGEDAGPYLDALTTLRREMERITERGIVVRDAESGLVDFPAEREGRAVFLCW
                     RLGEEAVAWWHPPETGFPGRRPL"
     gene            complement(3067..3771)
                     /locus_tag="VEO00_06500"
     CDS             complement(3067..3771)
                     /locus_tag="VEO00_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYU57683.1"
                     /translation="MPIEAVFFDAGETLIHPDPSFPELFAAVMADAGHPLEPEVVSQT
                     ATVASKRFQELATTRTLWSTNAEVSRAFWLELYADFLKGMGIDSDHAALAEALYETFS
                     DPGSYAVFPDAIPTLETLRDRGLALGLISNFEEWLELVLEARELTPFFPVRVISGSVG
                     VEKPDPQIFRIALERAGARAEDAVYVGDHPFFDVEAAEAVGMRGVLIDRRERYPDHPG
                     PRLPDLKGLPDLLETL"
     gene            complement(3865..4317)
                     /locus_tag="VEO00_06505"
     CDS             complement(3865..4317)
                     /locus_tag="VEO00_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU57684.1"
                     /translation="MVVNPIPESYQRVTPYLLVDGAAGEIDFLVAAFDAEERGERMPG
                     PNDTIGHAEVNVGDSVIMLADAGEQFPATRALSLVYVENVDATYERAMAAGATSDQEP
                     ETQFYGDRTANITDPFGQRWTIASHVEEVPPDEMGRRVQALLEQAQDA"
     gene            4422..5315
                     /locus_tag="VEO00_06510"
     CDS             4422..5315
                     /locus_tag="VEO00_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYU57685.1"
                     /translation="MDVRTVTIHGHHTAYRQLGSGPVVLLVHGMASSSSTWQGVIPRL
                     AERSTVIAPDLLGHGGSAKDATDHTLGGLASSLRDLLVLLGHERATLVGHSLGGGVAM
                     QFAYQFPERVERLVLVSSGGLGKEVAFILRALALPGVEYLLPPAFAPGLWRAGATVAS
                     WLGRMRVPRAAAIEEIWEAYGSLTDPDTRRAFFQVLRAVIGADGQRVSATDRLYLAAH
                     VPTLIVWGDRDGIIPVSHGRAAHETMPGSVLRVFEGVGHFPQRECPEEFTEALLEFLA
                     ATEPASLSGAGLRELLEAVEP"
     gene            5207..6502
                     /locus_tag="VEO00_06515"
     CDS             5207..6502
                     /locus_tag="VEO00_06515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU57686.1"
                     /translation="MSGGVHRGAARVPGGHRAGVAQWRGASGAPGGRGALTGPIADPP
                     ETRRTPRAARISIALAFAIHAAAAGTLGPRIPALKARSALSDGDLGIALTAFAAGLFA
                     GARLAAPLLRRAGSRRVIRLGTPVLCAALAFTGAVRGLVALAAALALFGALSGLLDVA
                     NNAQAVAVERSYGRPIISGIHATWSAGLLVSGAVGAILAALDVPLPVNVATMATLLAA
                     VSFPALRALLPTEPRDSSGEGGPPPRRMIPILGAVAFASFMVEGAAVDWSAVHLRDSL
                     GASAGVAGVGFAAFAGGMTVSRLVADRAVRLLGPTKVVRTAGVVGGAGFAAAALAGAT
                     GMAVVAFALVGVAMAPVVPEVFSAAGNAPRGHVVLGWVATMGYVGAVLGPAVIGAVAE
                     WTSLRTALLVPAGAALLVALLGRIRSPARPAAPAPHAAV"
     gene            6642..7127
                     /locus_tag="VEO00_06520"
     CDS             6642..7127
                     /locus_tag="VEO00_06520"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYU57687.1"
                     /translation="MTAEGEGAGMAPAAVEALRERARRDVEDGILPSCQIALAVDGRV
                     ALFETFGRDRRDPRYQVYSVTKALVAGAVWILLGEGRLATDTRVAEVIPGFGTNGKDA
                     VRVEHLLTHTAGFPRAPMRPLEGADRERRMARFPEWRLDWEPGTRFEYHPRRRTGCSR
                     S"
     gene            7817..8905
                     /locus_tag="VEO00_06525"
     CDS             7817..8905
                     /locus_tag="VEO00_06525"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HYU57688.1"
                     /translation="MELDDVAVRRLRQRAQLLHRPGRLSALGVVRRLVGIQAQDRATF
                     RLAIRARSASPIAADVDRALWSRRSIVRSWLMRGTLHLVASEDYPWIRLLTAEPVRNY
                     PRLRLRQEGVRDADVERGLRAIRRMLANDGPLTRQEIADRLARRGIHTEGQAAPWFLA
                     LASIQGIVCQGPDRGARSTFVLVDDWLGRPQRPVDRDAALAEFARRYLLSHAPAAPDD
                     FQAWSGLGLRDARRAWSAIAGSLTEARWRDRTLWMPRRTNRRAPRGPVRLASSFEEYL
                     LGWKGREATLPPEHAAVVVPGGGLLKPCLIVDGLVAGAWRIERRRDGITVEARPFAGA
                     GPSLRRALHAEASDVGRFLGAPAELRLA"
     gene            complement(8914..9501)
                     /locus_tag="VEO00_06530"
     CDS             complement(8914..9501)
                     /locus_tag="VEO00_06530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HYU57689.1"
                     /translation="MTTPHVVGIGGTTRDGSLTDKALRFALKTCQAEGATTTVFDGAF
                     LETLPHYAPDRFERAEGAIRLIDEIRRADGLIVASPGYHGGVSGLVKNAIDYAEDLRD
                     DPSPYLSGKPVGLIATGAGWQGIVTTIEALRAIVHALRGWPTPLGAAVNSAFSRFDQN
                     GDPSDEKVLSALTTVAEEVMQFVRRHQADLQTTED"
     gene            complement(9586..10251)
                     /locus_tag="VEO00_06535"
     CDS             complement(9586..10251)
                     /locus_tag="VEO00_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019359517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HYU57690.1"
                     /translation="MATQKALRAVPEDLPAVDGRVPGRRGKATRERLLRCTAQLLQKT
                     SYRDLSVIDIAQCAGTSPATFYQYFQDVEAAVLILAEALRAEGRRLAAIIEGSDWEGL
                     AGPDTAAALTDAFLGLWEDHRAVLRVVDLSIAEGDQRFRNVRTHMLNGVTESLRTVID
                     DFRHRDAHPKDLDPMAQAAALVSLLAHVAAHRYSFGFWGIKTEDMRRSVAGLVLAGVT
                     GTP"
     gene            complement(10336..10587)
                     /locus_tag="VEO00_06540"
     CDS             complement(10336..10587)
                     /locus_tag="VEO00_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HYU57691.1"
                     /translation="MTGDGRVASWTVIHPPVLPVWEDAVPFVVLLVELDEGVRMIGQL
                     VDATGERAGTDDVAFGSRASLVWRTDEAGQALPAWMLTP"
     gene            complement(10764..>11670)
                     /locus_tag="VEO00_06545"
     CDS             complement(10764..>11670)
                     /locus_tag="VEO00_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HYU57692.1"
                     /translation="VDDGGGAGAPTIQPAAMAIELGLAEVVVAYRSRNRSSGGRTWAV
                     QMLAAGQGQFERPHGVVRPADGFALIARVWMHRYGWTREDLGRVAIAIREHALRNPAA
                     MMHGHPLTMEEYLGAPMVAEPLCVHDTCLETDGALAMVLTSAERAADLDVVPAYVTGY
                     AMGSGPDLYTVTNVYGTEIGTTPNAFIAPDLWRNTGLGPEDVDVVQFYDAFTPQIPVA
                     FQEFGFCADGEGAAYLVGGEAPPYNTSGGGLSEAYTHGFNLLVEGVRQIRETSTDQVA
                     GAAHALVTSGNVVPTGAIVFSREPR"
BASE COUNT         1500 a         4209 c         4290 g         1671 t
ORIGIN      
        1 gctcccgccg ccacgctccc cggccgggcg tcgagcgtga cctccccggt gacccgtccg
       61 gtggtcatgt cgaccagccc caccgagttg ggcgtgacgg tccgggtggg acccccgtct
      121 ccgttaccgg agaccaggac cgggatcgtg acggccgcgg cgacgagagc gagcgcggcc
      181 cccaccgcca cggccaccct ggcgcgcgga gcgaagcggc gagggggccc ggagggcggg
      241 cgggtcgcca gaggagcgag ccgcagcgcc gcgtcctcgt gctcggaggc cacccggatg
      301 ggccggaccg gctgggcgat ccccttcagg tggagttcgc cccgctcggt gaaggtcagg
      361 ccgtcgatcc gccccgcgag gtggacggtc tcccggctgg ccaggacgtc gccgggcccg
      421 gcctcgccgc acaggcgggc cgcgaggttc agggcccggc cgcggtagcc cccctccacg
      481 ggcacggcct ctccgacgtc cagcccgatc cccaccagca gcgggagaga cgggtcgctc
      541 tccgtctcct cgacgaatcg agcctggagg tcgatcgcga cgcggagagc ctggcgcgcc
      601 gagccgaaca cggacagggc ctcgtctccc cgcagctcga ggaggatccc gcctctcgac
      661 tcgatctcct cccgagagat cgcggcgaac ttcgcggcga gcttggccgc cgcctcgtcc
      721 ccgcgctcct gggagaacag cgtgtatccg cggacgtccg cgatcaggaa ggtcctgatc
      781 tcggtacgac cctgatcatc gggcgagctc aacggcgact ccctcggcgt gcgcgcagat
      841 tatcgagcga aggcggcctc ctgaccaggc atcctcccgc tctcgtcaga gctcgtgagc
      901 gtctacttgc gcggttccca ggagaagaag cgcgtggcca ggaggacgcc ggcgacgccc
      961 cacgcgccga cgatgccgac gtcgctccag gcgaagccgt tcccggtcgg agcgaagtag
     1021 gcggactgca tggcctgcgc gaagtggtag atcgggaacg ccttcgccac cgtgacgtac
     1081 cacgcggtcg tgtcccgcag cggcacgaag atcccggaca ggaacagcag cggcaggatc
     1141 gaggcgttga cgacggcagg ggacgcctcg gcgttcggga tcgcagcggt catcgccagc
     1201 ccgagcgcgg agaagctccc ggccccgacg acgatggcga tcaggaacgc cggcagggtc
     1261 cgggtcggca gctccgcgtc gtagaaggcg gccccgaacg ccgcgacgat gaccaccagc
     1321 aggagggcca gcaggatcgc gtggacgatc cttccggaca tgaacgccca cgacggcagc
     1381 ggggtcccgc gcgtgcgctt caggatcccc gcgtcgcggg agaacgagac gctgatcgcc
     1441 agattcgtgt acgtcgcggt gatgatcgag aacgtggcca ccgccgagac gtagtaggtc
     1501 gagacgcgga tcgtccggcc gaggaccggg atcgtgtcgt tgccgaacag cgtggtgaag
     1561 atcaccagga acatcagcgg gaaggcgaac gtgaagaacg ccgatgccgg gttccgccag
     1621 aacagcttgt tctggtactt cacctgccgt gcggcgagtc cgaggtcgct cattcggttg
     1681 cgtggggagg ggaccctccc caccctccac ccgtcaggac cagccgtggg tgtctcaccc
     1741 ctcctccggt ccggcgtcgc cgccggtgat ctcgaggtag acgtcctcca gcgacggctt
     1801 cgtcacgtgc aggttggcga gctcgatccc acggccgatc gcccagccgg tcagctcgtg
     1861 cagcagccgc gtcggctcgg gggtggtgag caccacgtca ccttcgtggt cggaggccgc
     1921 cgaccggaga gggtcgggga gttcgccgac gtccgcctcg gggagccgga accggatccg
     1981 cgtgtccgcc gtgtcacgcc caccgagcgt ggtcgggtga ccgcgcgcca cgatctcgcc
     2041 gcggttgatg atcgccacgt cgtcggccag cgcctgcgcc tcgtccatgt agtgggtggt
     2101 cagcaggacc gtcttgccca gcgcgcggag gttgcggatc atgtcccagg cgttgcgccg
     2161 cgcgcccggg tcgaaccccg tggtcggctc gtcgaggaag agcaggacgg ggtcgccggc
     2221 cagcgcgatc gccacgtcca ggcgccgctg ctgcccgccc gacaacttgc tgacccggcc
     2281 atcgcgcttc tcgctgaggc ccacgacctc gacgatctcg tccaccggcc gcggatgcgg
     2341 gtagtagccc cggtacatct cgatcgtctc ggcaaccgtc aggtacggct cgatcccggt
     2401 cgactgcagg acgatcccga tccgctcctt caggtggcgc gggccggccg cggggtcgaa
     2461 cccgagcacg tcgacgtccc cgccgtcgcg cgggcgaaac ccctcgagga tctcgacggt
     2521 cgtggtcttg ccggcgccgt tcgggccgag cagcgcgaag acctcgccgg cccggaccgt
     2581 caggtcgatg ccgcgcaccg cctcgacctc gccgtaggcc ttccggagcc cgcggacgct
     2641 gatcgcgggg ccgccgtcgg aggatctcac gcggccatct tcccatcggc gctcacagcg
     2701 ggcgccttcc ggggaagccg gtctccggcg gatgccacca cgcgacggcc tcctccccca
     2761 gccgccagca gaggaacacg gcccggccct cccgctcggc ggggaagtcc accagtcccg
     2821 actccgcgtc gcgcacgacg atcccgcgct ccgtgatgcg ctccatctcc cggcgcaggg
     2881 tggtcagggc gtcgaggtag ggtccggcgt cctcgccgcc gccgtccgag ggcgcggcgc
     2941 tccggatccg ctcgccggag cgcaggatga cctgccgggc ctcgcggatc cgcgccagcg
     3001 ccgtccgcag ctcctcgagc tcggcgttcg cctcctcaac ggtgaaccgt cgctcccctt
     3061 cccgagtcat agcgtctcga gcaggtccgg gagtcccttc aggtccggga ggcgggggcc
     3121 cggatggtcg ggataccgct cgcggcggtc gatcaggacg ccgcgcatgc cgaccgcctc
     3181 cgccgcctcg acgtcgaaga acgggtggtc cccgacgtag accgcgtcct cggcccgcgc
     3241 gccggcccgt tccagcgcga tccggaagat ctgcgggtcg ggcttctcca cgcccaccga
     3301 tcccgagatc acgcgcaccg ggaagaacgg cgtcagctcg cgggcctcga gcaccaactc
     3361 cagccactcc tcgaagttcg agatcaggcc gagcgccaga ccgcggtcgc gcagcgtctc
     3421 gagcgtcggg atggcgtcgg ggaacacggc gtaggagccc gggtcggaga acgtctcgta
     3481 gagcgcctcg gcaagtgccg cgtggtcgct gtcgatcccc atgcccttca ggaagtcggc
     3541 gtacagttcc agccagaacg cgcgcgagac ctcggcgttc gtcgaccaca gcgtccgggt
     3601 ggtggccagc tcctggaatc gcttcgacgc cacggtggcc gtctggctga cgacctcggg
     3661 ctcgaggggg tggccggcgt cggccatcac cgccgcgaag agctccggga acgaggggtc
     3721 cgggtggatc agcgtctcgc cggcgtcgaa gaacaccgcc tcgatgggca cgggccgatg
     3781 ataggcatgc gtgcgtcgtg cgggagatcg aggcgacagg cgacggccgc ggcagttgac
     3841 gcgccggtcc tctgatccgc cgcgtcaggc gtcctgggcc tgctccagca gcgcctgcac
     3901 gcgccggccc atctcgtcgg gcggtacctc ctcgacgtgc gaggcgatcg tccagcgctg
     3961 cccgaagggg tcggtgatgt tcgcggtgcg gtcgccgtag aactgcgtct ccggctcctg
     4021 gtcggacgtc gccccggcgg ccatcgcccg ctcgtaggtg gcatcgacgt tctccacgta
     4081 cacgaggctg agggcgcggg tcgcggggaa ctgctcgccc gcgtcggcga gcatgatgac
     4141 cgagtcgccc acgttcacct cggcgtgccc gatcgtgtcg ttcgggcccg gcatccgctc
     4201 cccacgctcc tcggcatcga acgcggccac gaggaagtcg atctcgcccg cggcgccgtc
     4261 gacgaggaga tagggcgtca cgcgctggta gctctcgggg atcgggttca ctaccatgct
     4321 gcctcctcgg ctcgatggag cgccctgcga tcctacggcc ggacggcgac gcgcgtaccg
     4381 gcatgcgacg cccagcggtc gggcagggta cggttcgtcc gatggacgtc cgcacggtga
     4441 ccatccacgg gcaccacacg gcgtaccggc agctgggctc ggggccggtg gttctgctcg
     4501 tgcacgggat ggcgtcgagc tcgtcgacgt ggcagggcgt gatcccgcgg ctggccgagc
     4561 gctccacggt catcgcgccc gacctcctgg ggcacggcgg gtccgccaag gacgccaccg
     4621 atcacacgct cggcggcctg gcgagctccc tccgtgacct gctcgtcctg ctcgggcacg
     4681 agcgggccac cctggtgggg cactcgctcg gcgggggcgt ggcgatgcag ttcgcgtacc
     4741 agttcccgga acgggtcgag cgactggtgc tcgtgtcgag cggcggtctc ggcaaggagg
     4801 tcgcgttcat cctgcgcgcg ctggcgttgc cgggcgtcga gtacctgctg ccgcccgcgt
     4861 tcgcgccggg gctctggcgc gccggggcaa cggtcgcgtc gtggctcggc cggatgcgcg
     4921 tgccgcgcgc cgccgcgatc gaggagatct gggaggcgta cggctccctg accgacccgg
     4981 acacgcggcg ggccttcttc caggtcctgc gcgccgtgat cggcgccgac ggtcagcgcg
     5041 tcagcgcgac cgaccggctg tacctggccg cccacgtgcc gacgctgatc gtgtggggtg
     5101 accgcgacgg gatcatcccg gtctcgcacg gccgcgcggc gcacgagacc atgcccggga
     5161 gcgtcctgcg cgtgttcgag ggcgtcgggc acttcccgca gcgtgagtgt ccggaggagt
     5221 tcaccgaggc gctgctcgag ttcctggcgg ccaccgagcc ggcgtcgctc agtggcgcgg
     5281 ggcttcggga gctcctggag gccgtggagc cctgaccggc cccatcgccg atcccccgga
     5341 gacgcggcgc acaccacgcg cggcgcggat ctccatcgcg ctcgcgttcg cgatacacgc
     5401 ggccgccgcc ggcacgctcg gaccccgcat tccggcgctg aaggcccggt cggcgctgtc
     5461 ggacggcgac ctcgggatcg ccctgaccgc gttcgcggcg ggcctgttcg ccggcgctcg
     5521 gctggccgcc ccgctgctgc gccgggccgg gagccgtcgg gtgatccgcc tggggacgcc
     5581 ggtgctctgt gccgccctcg cgttcaccgg tgccgtccgc gggctcgtcg cgctggcggc
     5641 ggcgctcgcc ctgttcggcg ccctgagcgg cctcctcgac gtggcgaaca acgcccaggc
     5701 cgtggcggtc gagcgctcct acgggcgacc gatcatctcg gggatccacg cgacgtggag
     5761 tgccggactg ctcgtctcgg gggccgtcgg agcaatcctc gcggccctcg atgttccgct
     5821 gcccgtgaac gtcgccacga tggcgacgct gctggccgcg gtgtcgttcc cggcgcttcg
     5881 cgccctcctg ccgacggagc cgcgtgattc atccggcgaa ggcgggccgc ccccgcgaag
     5941 gatgatcccg atccttggcg ccgtcgcgtt cgcgtcgttc atggtggagg gggccgcggt
     6001 ggactggagc gccgtccacc tgcgtgattc gctcggcgcc tcggccggtg tggcgggggt
     6061 cgggttcgcc gcgttcgccg gcgggatgac cgtctccagg ctcgtcgccg accgggcggt
     6121 gcgcctgctc gggccgacga aggtggtgcg aacggccggt gtcgtcggcg gcgctggctt
     6181 cgccgccgcg gcactcgccg gggccaccgg catggccgtc gtcgcgttcg ccctcgtcgg
     6241 cgtggccatg gcgccggtgg tcccggaggt cttctccgcc gccgggaacg ccccccgcgg
     6301 gcacgtcgtg ctcggctggg tcgcgacgat gggatacgtc ggtgccgtac tcgggcccgc
     6361 cgtgatcggc gcggtggccg agtggaccag cctgcggacg gccctcctgg tccccgccgg
     6421 ggcggcgttg ctggtggccc tgctggggcg gatccggtcg ccggcccgcc cggccgcccc
     6481 cgccccccac gccgcggtct gacagcaggt gtcgcgcctc ccgggagttc tgacggtccg
     6541 tcagattccg gtacgatttc gcgggagacc atggcccacc ccttcatcca gtggcggcac
     6601 acgtcacaca gcccgcttct ccggcgcggg gtagaggggc gatgaccgcc gagggggagg
     6661 gcgccggcat ggctccggcc gcggtcgagg ccctcaggga acgggcgcgg cgcgacgtgg
     6721 aggacggaat tctcccgtcg tgccagatcg ccctcgctgt cgacgggcgg gtggcgctct
     6781 tcgagacctt cggccgggac cgccgggacc cccgttacca ggtctactcc gtgacgaagg
     6841 cactcgtggc cggggccgtg tggatcctgc tcggcgaggg tcggctggcg accgacacgc
     6901 gggtggcgga ggtgatcccc gggttcggca cgaacggcaa ggacgccgtc cgcgtcgagc
     6961 atctcctgac gcacacggcc ggcttccccc gcgcgccgat gcgcccgctc gagggcgccg
     7021 accgcgagcg gcgcatggcc cggttcccgg agtggcgcct cgattgggag ccgggcacgc
     7081 gcttcgagta ccacccacgt cggcgcactg ggtgctcgcg gagttgatcg atcgcgcggg
     7141 ggcgaccgac caccgccgtt acgtggccga gcgcgtgatg gagcccctcg ggctcgcgcg
     7201 gctgcggctc ggcgtccccc gcggcagcag ggggacgtcc tggaggccgt cgcggtcggc
     7261 cagatcgacc cggagaccct gcggctcgtg gagcggatcg acgagagcga gctgatgctg
     7321 aaggagctgc gccaggggaa cctgctgcgc ttcaacgacc cggccgtgcg cgaggcgggg
     7381 gtgcccggcg ccggcgcgat cggcaccgcg gccgacgtgg cggcctactt ccaagcgctg
     7441 ctgcgcgacc cggccggcct gtgggatccc gccgtcctgg aggccgggac acgaacggtc
     7501 cgcaccgcct tcccgacccg tggaccggga tcccggcgaa ccgcacgctc gggctcgtgg
     7561 cggcggggga cgacgggaac gcggtgctgc gcggcttcgg gcagggcgtc gggccccgag
     7621 cgttcggggc gacggggatc ggcggccagg ttgcgtgggc cgaccccgac tcaggcctgt
     7681 ccttctgcta cctcacgaac gggctggacg tggacccggt gcgcgcgttt cggcggcagg
     7741 ccgacctgtc cggctaccgc cgcggcgtgc gtcccagcgt aggcggaggg ctcgcgcgcg
     7801 ttcgtacgat ggctccgtgg aactcgacga cgtcgccgtt cggcggctgc ggcagcgcgc
     7861 acagctgttg caccgcccgg ggcggctgtc ggcgctcggg gtggtgcggc ggctggtggg
     7921 gatccaggcg caggaccggg ccaccttccg gctggcgatc cgggcgcgaa gcgcctcgcc
     7981 gatcgcggcg gacgtcgacc gggcgctctg gagccggcgc tcgatcgtgc ggagctggtt
     8041 gatgcgcgga acgctgcacc tggtcgcctc ggaggactac ccgtggatcc gcctcctcac
     8101 ggccgagccg gtgcggaact acccgcggct gcggctgcgg caggagggcg tccgggacgc
     8161 cgacgtcgag cgcgggctgc gagcgatccg ccgaatgctg gcgaacgacg ggccgctgac
     8221 ccggcaggag atcgccgatc ggctggcccg gcgggggatc cacaccgagg ggcaggccgc
     8281 gccctggttt ctggcgctgg cgtccatcca ggggatcgtc tgccagggcc cggatcgcgg
     8341 cgctcggtcg acgttcgtcc tcgtcgatga ctggctgggc cggcctcagc gtccggtcga
     8401 ccgcgatgcg gcgctcgcgg agttcgcccg gcgttacctc ctctcgcacg cacctgcggc
     8461 gcccgacgac ttccaggcgt ggtcaggcct gggcctgcgg gacgcccggc gcgcctggag
     8521 cgcgatcgcc ggttccctga cggaggctcg ctggagggac cggacgctgt ggatgccgcg
     8581 aaggacgaat cgtcgggcgc cgcgcggccc ggttcggctc gcgagctcgt tcgaggagta
     8641 cctgttgggg tggaagggtc gcgaggccac gcttcccccg gagcacgcgg ccgtggtcgt
     8701 tcccggcggg ggcctgctga agccctgcct gatcgtcgac gggctggtgg cgggcgcctg
     8761 gcggatcgag cgccggcggg acgggatcac cgtggaggcg cggcccttcg ccggggcggg
     8821 cccgagcctc cgccgcgcgc tgcacgccga ggcgagcgac gtcggccggt tcctcggggc
     8881 gcccgccgag ctccggctgg cgtgacgcgg ccctcagtcc tcggtcgtct gcagatcggc
     8941 ctggtgccgc cgtacgaact gcatgacctc ctcagcgacg gtcgtcagcg ccgacaggac
     9001 cttctcgtcg gacgggtcgc cgttctggtc gaagcgggag aaggcggagt tcaccgcggc
     9061 gccgagcggc gtgggccagc cgcgcagcgc gtggacgatc gcgcgcaggg cttcgatggt
     9121 cgtcacgatc ccctgccagc cggcgccggt cgcgatcagc ccgacgggct tgccggagag
     9181 gtacgggctc gggtcgtcgc gcaggtcctc ggcgtagtcg atggcgttct tcaccaggcc
     9241 ggagacaccg ccgtggtacc cgggcgaggc gacgatcagg ccgtcggcgc gccggatctc
     9301 atcgatcagg cggatcgccc cctcggcccg ctcgaaccga tcgggtgcgt agtgcggcag
     9361 ggtctccagg aacgcgccgt cgaacacggt cgtcgtggcg ccctcggcct ggcacgtctt
     9421 cagcgcgaag cggagcgcct tgtcggtcaa ggagccgtcg cgcgtggtcc cgccgatgcc
     9481 gacgacgtgg ggcgtggtca cccctctccc atctccacca ccgcgagatc cagcaggatc
     9541 cgggtgctcg aggcgaggtt cgcgtccacc gtatcgcgcc aggtgtcagg gcgttccggt
     9601 cacgccggcg aggaccaggc cggccacgct gcggcgcatg tcctcggtct tgatccccca
     9661 gaacccgaag ctgtagcggt gcgcggcgac gtgggccagc agcgagacga gggcggcggc
     9721 ctgggccatg ggatcgaggt ccttcgggtg cgcgtcgcgg tggcggaagt cgtcgatcac
     9781 ggtgcgcagg ctctccgtga cgccgttcag catgtgggtg cggacgttgc ggaagcgctg
     9841 gtcgccctct gcgatcgaga ggtcgacgac gcgcagcacg gctcggtggt cttcccacag
     9901 gccgaggaac gcgtcggtca gcgccgccgc ggtgtcgggg ccggcgagtc cttcccagtc
     9961 cgagccctcg atgatcgccg cgagccggcg accctcggcc cgcagcgcct cggccaggat
    10021 cagcaccgcg gcctcgacgt cctggaagta ctggtagaag gtcgccggcg aggtccccgc
    10081 gcactgggcg atgtcgatca ccgacaggtc acggtacgac gtcttctgca ggagctgcgc
    10141 ggtgcagcgc agcaggcgct cgcgggtggc cttcccgcgg cgccccggga cccgcccgtc
    10201 gacggccggt aggtcctccg gcaccgcccg cagtgccttc tgcgtggcca tcagccgccc
    10261 ctcgtccccc tccccaggaa atctgacgga ccgtcacatc atacgaagcc agctcgagct
    10321 gccgccacgc gcccgctacg gagtcagcat ccaggccggg agggcctgcc cggcctcgtc
    10381 ggtacgccag acgagggagg cgcggcttcc gaacgccacg tcgtccgttc ccgcgcgttc
    10441 gccggtggcg tcaacgagct gcccgatcat gcgcacaccc tcgtcgagct cgaccaggag
    10501 cacgacgaac gggaccgcgt cctcccagac gggcagcacc ggcggatgga tcaccgtcca
    10561 cgaggcgacc cggccgtcgc cggtcacctc ggtccacgtc ggatcgtccg tgcggcagcg
    10621 cgggcagacg ggcttcggtt gccagatcca catccggcac ccggcgcaac gctgaacgag
    10681 cagccggcgc ctggcgacgc cgtcccagaa cggttgagta tcggtgtccg ggaccggtcg
    10741 cggcggcagc ggcgcaggct tgctcaccgc ggttcccggc tgaacacgat cgcgcccgtc
    10801 ggcacgacgt tgccgctcgt caccagcgcg tgcgccgcgc ccgcgacctg gtcggtggag
    10861 gtctcgcgga tctggcggac cccctcgacg agcaggttga agccgtgcgt gtacgcctcc
    10921 gacagcccgc cgccgctcgt gttgtacgga ggtgcctcgc cgcccacgag gtacgcggcg
    10981 ccctcgccgt cggcgcagaa gccgaactcc tggaacgcca ccgggatctg cggcgtgaac
    11041 gcgtcgtaga actggacgac gtccacgtcc tccggaccga ggcctgtgtt ccgccacagg
    11101 tccggagcga tgaaggcgtt tggcgtcgtg ccgatctcgg tgccgtacac gttcgtcacg
    11161 gtgtagaggt ccggaccgct ccccatggcg tacccggtga cgtacgccgg gaccacgtcc
    11221 aggtcggcgg cgcgctcggc gctcgtcagg accatcgcca gcgccccgtc ggtctccaga
    11281 cacgtgtcgt gcacgcagag cggctccgca accatgggtg cgccgagata ctcctccatc
    11341 gtcagcggat ggccgtgcat catcgccgcc gggttgcgca gcgcgtgctc gcgtatcgcg
    11401 atggccaccc ggccgaggtc ctcgcgggtc cagccgtagc ggtgcatcca cacgcgggcg
    11461 atgagggcga acccgtcggc cggccggacg acgccgtgcg gccgctcgaa ttggccctgg
    11521 cccgctgcga gcatctgcac ggcccacgtg cggccgcccg aggaacggtt tcgcgaccga
    11581 taggccacga ccacctcggc caggccgagc tcgatggcca tcgccgccgg ctggatggtc
    11641 ggcgccccgg cgcccccgcc gtcgtcgacc
//
LOCUS       DASPWO010000287         9791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_300199, whole genome shotgun sequence.
ACCESSION   DASPWO010000287 DASPWO010000000
VERSION     DASPWO010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300199"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VEO00_06550"
     CDS             complement(<1..537)
                     /locus_tag="VEO00_06550"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HYU57693.1"
                     /translation="MSALARPLIAAVVVDAAHDTGAEAVAHGCTGKGNDQVRFETTFA
                     ALAPDLRVLAPVREHALSREEALAKAAAWGIPIPAESRVYSVDENLWGRTIECGPLED
                     PWTEPLAEAFAITADPLLAPDEPAEAVVAFAGGVPVALDGVGLPALEIVPRVAALAGR
                     HGFGRVDMIENRLVGIKSR"
     gene            complement(615..1862)
                     /locus_tag="VEO00_06555"
     CDS             complement(615..1862)
                     /locus_tag="VEO00_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYU57694.1"
                     /translation="MGGAAARLARQDVAWERIGTLPGRFRRPAAEGLTAEIQITVDDR
                     PFTISIVDGECFVRRGKSIRADTRIDTDVETWLSLDDGVLVTADLFLGGRLRVRGNVD
                     LAVRMQALFEPHGRERTVADLQQVTVRANGVALSAYMLGEGPPLLLLHGLGGAKVSWM
                     PLLAPLSERYRVIAPDLPGHGESDKPRQAEYSPRYYAAVVRKLMDTLDARSAHVVGNS
                     MGGRVALELAARSPKRIRGLALLDPALPGLRWRWFMGFMQVAPVRRGLGAIPFPANER
                     WGQLMLRRLFAQPDRFPRHAYEAGADEFARVYRSARARLAFWASLRHVIYDSGEEFWR
                     LIGRVQAPTLILWGERDRLVPLRLGNQLARAIPHAEYLVLPDVGHVPQFEAPKSTSQA
                     LGRFLHAIDAAGDGEVGGPAGPG"
     gene            1970..2386
                     /locus_tag="VEO00_06560"
     CDS             1970..2386
                     /locus_tag="VEO00_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYU57695.1"
                     /translation="MTELLPQKGIIMILARELASKLATATFLVEPDGTLVYYNEPAEK
                     ILGVEFAEAGPLKPDEWGTMFSPVDEQGNPLPLEKLPLAVTLASKRPSHRTFRITGMD
                     GVTRAIAVTAIPLFAREDEFVGGVAFFWEEGGPAAA"
     gene            2411..3262
                     /locus_tag="VEO00_06565"
     CDS             2411..3262
                     /locus_tag="VEO00_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYU57696.1"
                     /translation="MRVKIWGCRGSLAAPGPDTVKYGGNTSCVEVRLDDDSLVILDAG
                     TGIRPFGLALGDDRPPVIHLLLSHLHLDHLEGLGFFSPLWSPDVELHVWGPPSPLRSL
                     EERIGRYLSPPLFPIHLSDIPSHPTFHNVPEGEFQIGSAWIFSQPVSHPGPTVGYRIE
                     ENGRKLAYIPDHEPALGVDLASVSPDWISGYSIALGADVLLHDAQFSEEEYPTKIGWG
                     HSSTRDLVTFATIAKVDHLVMFHHDPLHTDAELEAIQARAEELWGEDGGEPPVLAYEG
                     MTIDLSG"
     gene            3326..4114
                     /locus_tag="VEO00_06570"
     CDS             3326..4114
                     /locus_tag="VEO00_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYU57697.1"
                     /translation="MIWRIAATVVVGVATGLLSAMFGVGGAVVSTPAIRALGASALEA
                     VGTTLPSILPSAVTGTLRYRRAGLVEWRIVAVSGPAGAAAAVGGSFLSRAVPGRGHLL
                     MVATAVLLGFAAVRAGLGRHEAVEPAEEEDQARPRRRVPGWRRAVIGLIAGGLSGLLG
                     VGGGIVLVPAFTAWLGLPIKRAVGTSLACVGIIAVPGTLTHAFLGDIDWAFAVPLSVA
                     VVPGAAIGARLAIRATDRTLRGVVAVALGGIAVLYGLLELLSLR"
     gene            4278..4784
                     /locus_tag="VEO00_06575"
     CDS             4278..4784
                     /locus_tag="VEO00_06575"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYU57698.1"
                     /translation="MLSFGDELSGYDLLKGIQGSVAYFWSPAKSHVYAELRRLLRLGW
                     ATERAVEQDARPDKRVYRITHRGRTALRAWLADPGVEPDTYRSPLLLKLFFGAEADRE
                     TLVTRVKDYREAARAQLAEFRAIERRIAGDRDALFPSLTLRAGLAHTRAAERWADDVL
                     RTLEEESR"
     gene            4781..5758
                     /locus_tag="VEO00_06580"
     CDS             4781..5758
                     /locus_tag="VEO00_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010272522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU57699.1"
                     /translation="MSAVTRGADAGTTVAVEVEDLVKTFPAPGGGRLAAVNGVSFAVE
                     RGEVFGFLGPNGAGKTTTLEIIEGLQSPTSGRTAVLGLDSQQDRNRMKERIGVQLQAG
                     AYFDYLTLEEILELFGSFYPTALRPDPLLEKVGLLEKRKALVRELSGGQAQRFSIVAS
                     MVNDPDVLFLDEPTTGLDPQARRNLWEFIGSINRDEGKTIVMTSHYMEEAQVLCDRVA
                     IIDHGEIQALDTPLGLIHTLPTAYRILFATQVPIDPGPLRELPGVAEVVAAQDGAFDF
                     ELRVSRAQVTLPAFIGWAERAGVQVDDVQVLPATLEDVFLSLTGRSLRE"
     gene            5764..6849
                     /locus_tag="VEO00_06585"
     CDS             5764..6849
                     /locus_tag="VEO00_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU57700.1"
                     /translation="MGKLLGASIRMLYRDRQALFWALAFPVIFAVIFGLFDFNQAPEV
                     GIAVVADRPSQVSRALVTGLREVESFEVEGRSSLASARTALEDGDVDVALEVPAIPAV
                     PETSTFAVRVLYNRSNFNVNQFALSAIERIVGGLNLRLAGVSSPPIAVRAEAVDAKSV
                     DYYDFLLPGLVAMGVMNFSIIGIAVGITRFREQRILKRMLATPLRPAKFLAAQVLARL
                     VLALAQAAVILAVGVFVFGGRVYGNVLWIFVLATVANLIFLNIGFTVAGRTGNVDAAQ
                     GLANFIALPMMFLSGVFFPTDTLPQAVQAVVKYLPLTPLIEALRKVSVDGLSIVDTGP
                     QLVKLGAWVVASFALAAASFRFGREAG"
     gene            complement(6853..7728)
                     /locus_tag="VEO00_06590"
     CDS             complement(6853..7728)
                     /locus_tag="VEO00_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU57701.1"
                     /translation="MSSLLVGTALRQAVRPGMDPVADARHVESLGFDFLSVTDHLVGR
                     PAYETWTHLTWAAAATERVGLLSDVLGLPYRHPALLAKMAEALQRLSGGRLLLGVGGG
                     GSDREFHALGLAVRRPAEKVDALRESIEVMRSLWTAPAATYDGAYFALRDAELEPKPE
                     PPIPVWVGSYGTRALGVTGRLGDGWLPSLPYLPPERYAERRDVVRRAAAKAGRDPDAI
                     VCGYNVSVAVGEGVTARPNSIAGPPGLVVEHLAALRGLGVTALNLWPALDPEDQRERL
                     AAEVLPGLRAAWREA"
     gene            complement(7725..8450)
                     /locus_tag="VEO00_06595"
     CDS             complement(7725..8450)
                     /locus_tag="VEO00_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HYU57702.1"
                     /translation="MITVRRDGEIHREEGGWFEARWHFSFDRYRDPENMGWGLLRVFN
                     DDRLIPGAIWPLHPHRDIEGLTYVVEGTFRHEDDLGNDGVLRAGSVQRMTLGSGAWHS
                     EQNGSESEPMRFIQMWVLPHERGLPPSVEQREFTVEDRTDRLLRAISGEGGEGGEAVH
                     VHQDASVFVARLNPGVSVAHAFSPGHGGYLYVIGGALRLGEDGEVLRTGDAVKVRDER
                     GLPLAAEETTELLLVDVPMADRP"
     gene            8512..9123
                     /locus_tag="VEO00_06600"
     CDS             8512..9123
                     /locus_tag="VEO00_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU57703.1"
                     /translation="MPKGSGIEVRPVTPDRWDDLAALFGIRGGYGHCWCMWWRVTSAE
                     FEGGIRERGRGNRRRMRTLVRDGAEPGLLAYRDGRPVGWVSVAPRDQFGRIERSRVLG
                     PVDDRPVWSIVCFFIGRAHRASGVGTALLEAAVRHAADRGATEVEAYPVDPAARVYSN
                     ADAYTGLLPMFLRAGFREVERRSAARPIVRKRIRRRARRARSG"
     gene            9299..>9791
                     /locus_tag="VEO00_06605"
     CDS             9299..>9791
                     /locus_tag="VEO00_06605"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57704.1"
                     /translation="MTAAAVGDTVYVVGGFVESGATVRRVEAYDGAAGAWSVAPPLPI
                     AANHAMSAGAGRRLVVAGGYRGPNLSNPTDRAFVFLDGRWRELPRMPETRAAGGAAVA
                     AGRLYVVGGTVTGTGDLARTTLVYTFASGRWSTAPGLPTRREHLGVAAFGGKVYAVGG
                     RTGG"
BASE COUNT         1292 a         3546 c         3583 g         1370 t
ORIGIN      
        1 gcgcgacttg atccccacca ggcggttctc gatcatgtcc acccggccga acccgtggcg
       61 cccggcgagc gcggccacgc gcgggacgat ctcgagcgcc ggcagcccga ccccgtcgag
      121 cgccacgggg acgccgcccg cgaaggcgac gacggcctcc gccggctcgt cgggcgccag
      181 aagggggtcg gcggtgatgg cgaacgcctc ggccagcggc tcggtccagg ggtcctccag
      241 gggcccgcac tcgatcgtcc gtccccacag gttctcgtcg accgagtaga ccctcgactc
      301 ggcggggatg gggatgcccc acgccgccgc cttcgccagc gcctcctcac gggacagcgc
      361 gtgctcgcgc acgggggcca ggacgcgcag gtccggggcg agcgcggcga acgtcgtctc
      421 gaagcggacc tggtcgttgc ccttgccggt gcatccgtgg gcgaccgcct cggcacccgt
      481 gtcgtgagcg gcgtccacga ccaccgcggc gatcagcggg cgcgccagcg ccgacaccag
      541 cggctacttc ccctcgtaga gcgcgttggc gacgagccgc gactgttccg ccacggcctg
      601 gggccggagg agcctcagcc gggcccggcg ggtcccccga cctcaccgtc gccggcggcg
      661 tcgatcgcgt gcaggaagcg tccgagggcc tggctcgtcg acttcggcgc ctcgaactgg
      721 gggacgtgcc cgacgtcggg caacaccagg tactcggcgt gtgggatcgc ccgagcgagc
      781 tggttcccga ggcgcagcgg aaccagccgg tcccgctccc cccacaggat cagcgttggg
      841 gcctggacgc ggcctatcag ccgccagaac tcctcccccg agtcgtagat cacgtggcgc
      901 agcgaggccc agaaggccag ccgggcgcgg gccgaacggt agacccgggc gaactcgtcg
      961 gcgccggcct cgtacgcgtg ccgcgggaac cggtcgggct gcgcgaacag ccggcgcagc
     1021 atgagctgcc cccagcgctc gttggcgggg aacgggatcg ccccgagccc gcgccggacc
     1081 ggggccacct gcatgaaccc catgaaccac cgccagcgca gcccgggcag cgcggggtcg
     1141 agcaacgcca gcccacggat ccgcttcggg gaccgtgccg ccagctcgag cgcgacccgg
     1201 ccacccatcg agttcccgac gacgtgcgcg ctgcgcgcgt ccagcgtgtc catcagcttg
     1261 cgtacgaccg ccgcgtagta gcgtgggctg tactcggcct gccggggctt gtccgactcg
     1321 ccgtggcccg ggaggtctgg ggcgatgaca cggtagcgct ccgagagcgg cgcgagcagg
     1381 ggcatccagg acaccttggc cccccccagc ccgtggagca ggagcagcgg cgggccctct
     1441 cccagcatgt acgcggagag ggcgacgccg ttcgcgcgga cggtcacctg ctggaggtcg
     1501 gcgacggtcc gctcccgccc gtggggctcg aagagcgcct gcatgcgcac cgccagatcc
     1561 acgttcccgc ggacgcgcag gcgaccgccg aggaacaggt ccgccgtgac cagcacgccg
     1621 tcgtcgagcg acagccacgt ctcgacgtcg gtgtcgatgc gggtgtcggc gcggatgctc
     1681 ttgccgcgcc ggacgaagca ctcgccgtcg acgatcgaga tcgtgaacgg ccgatcgtcg
     1741 acggtgatct ggatctcggc ggtgaggccc tcggcggccg gccggcggaa gcgccccggg
     1801 agcgtcccga tccgttccca cgcaacgtcc tgtcgcgcca accgcgccgc ggcaccgccc
     1861 atcgcccgga agggtactcg ctccgcccgc cccggcgggg gagcccgccc ggtgccgtga
     1921 tcggcgcgat gtgttgttcc cgggcgatcg gaggtcgtac cctgcagcga tgacggagct
     1981 cctgccgcag aaggggatca tcatgatcct cgcgcgcgag ctggcgtcga agctcgccac
     2041 ggcgaccttc ctggtggagc ccgacgggac gctcgtctac tacaacgagc ccgccgagaa
     2101 gatcctcggg gtggagttcg ccgaggccgg cccgctcaag cccgacgagt gggggacgat
     2161 gttctctccg gtggacgagc aggggaaccc gctgccgctg gagaagctcc ctctcgccgt
     2221 cacgctggcg tcgaaacggc cgtcacatcg gacatttcgg atcaccggca tggacggtgt
     2281 gacgcgcgcc attgccgtga cggccattcc gctgttcgct cgcgaggacg agttcgtcgg
     2341 cggggtggcg ttcttctggg aagagggcgg ccccgcggcc gcctgatcga ggaacggagg
     2401 accgggaccg gtgcgggtga agatctgggg gtgccgcggc tccctggccg cgccgggtcc
     2461 cgacacggtg aagtacggcg gcaacacctc ctgcgtcgag gtgcggctgg acgacgactc
     2521 gctggtcatc ctcgacgccg ggaccggcat ccggccgttc ggtctggcgc tcggcgacga
     2581 ccgcccgccc gtgatccacc tgctgctctc ccaccttcac ctcgatcacc tcgaggggct
     2641 cgggttcttc tcgccgctct ggagccccga cgtggagctg cacgtctggg gcccgccctc
     2701 gccgctgcga agcctggagg aacggatcgg gcgctacctc tccccgcccc tgttcccgat
     2761 ccacctgtcc gatatcccct cgcacccgac cttccacaac gtgccggagg gggagttcca
     2821 gatcgggagc gcgtggatct tctcccagcc cgtgtcgcac ccgggtccga ccgtcggcta
     2881 ccggatcgag gagaacggac ggaagctggc ctacatcccc gaccacgagc ccgcgctcgg
     2941 cgtcgacctg gcgtcggttt cgccggactg gatctccgga tactcgatcg ccctcggggc
     3001 cgacgtcctg ctgcacgacg cgcagttcag cgaggaggag tacccgacga agatcggctg
     3061 gggtcactcc agcacccgcg acctcgtgac gttcgccacc atcgcaaagg tcgaccacct
     3121 ggtgatgttc caccacgacc ccctgcacac cgacgcggag ctcgaggcga tccaggcccg
     3181 cgccgaggag ctgtggggcg aggacggcgg cgaacccccc gtgctcgcct acgaggggat
     3241 gacgatcgac ctctccggct gacggcgccg acgccccgca cggtccgccg acaccgccgg
     3301 cccctccccg gccggtgact cccccgtgat ctggcggatc gcggccaccg tggtcgtcgg
     3361 cgtggcgacc gggctgctct cggcgatgtt cggcgtcggg ggcgcggttg tctccacgcc
     3421 ggcgatccgg gcgctggggg cgagcgccct ggaggcggtg gggacgacgc ttccatcgat
     3481 cctgccgtcg gcggtcaccg ggacgctccg ctaccgccgc gccgggctgg tggagtggcg
     3541 gatcgtggcc gtcagcggcc ccgccggcgc ggcggcggcg gtcggcgggt cgttcctgtc
     3601 gcgcgccgtg cccgggcggg ggcacctgct gatggtggcc accgccgtgc tgctcggctt
     3661 cgccgccgtc cgcgccgggc tgggccgcca cgaggcggtc gagccggcgg aggaggagga
     3721 tcaagcgagg ccgcgacggc gggtcccggg gtggcggcgg gccgtgatcg gcctgatcgc
     3781 cggcggcctc tccgggctgc tcggcgtggg gggcgggatc gtcctggtcc cggcgttcac
     3841 cgcctggctc ggcctgccga tcaagcgcgc ggtcgggacc tcgctggcct gcgtcgggat
     3901 catcgccgtc cccggcaccc tcacgcacgc gttcctcggc gacatcgact gggcgttcgc
     3961 cgtcccgctc tccgttgcgg tcgtccccgg cgccgcgatc ggcgcccgcc tcgccatccg
     4021 cgccaccgac cggacgctgc gcggggtggt ggcggtcgcg ctcggcggca tcgccgttct
     4081 ctacggcctc ctggagctcc tctcgctgcg gtgacgggcg gcatccggcg accgcttgac
     4141 tcccgggcgg ccggccgtct atggttctgg ctgaactagt cctaaacgaa ctagaggggg
     4201 ggggaggggg aacgaaccga ggcggacgga cggccaaggg gaacggggtc ccgatcacta
     4261 cctacgcggt gctcgggatg ctctcgttcg gcgacgagct ctccggatac gacctgctga
     4321 aagggatcca ggggagcgtg gcctacttct ggtcgccggc gaagagccac gtctacgccg
     4381 agctgcgccg gctgctgcgg ctcggctggg ccaccgagcg agcggtcgag caggacgccc
     4441 ggcccgacaa gcgcgtgtac cggatcaccc accgcggccg gaccgcgctc cgcgcctggc
     4501 tggcggaccc cggggtcgag cccgacacgt accggagccc gctgctgctg aagctgttct
     4561 tcggggccga ggcggaccgc gagacgctcg tcacgcgggt gaaggactac cgggaggcgg
     4621 cgcgcgcgca gctcgcggag ttccgggcga tcgagcggcg gatcgccggt gaccgcgacg
     4681 ccctgttccc gtccctgacc ctgagggcgg gcctggccca cacccgcgcg gccgagcggt
     4741 gggccgacga cgtcctgcgc acgctcgagg aggagtctcg atgagcgctg tgacgcgcgg
     4801 cgcggacgcg ggtacgaccg tcgccgtcga ggtcgaggac ctcgtgaaga ccttccccgc
     4861 cccggggggc ggcaggctcg ccgcggtgaa cggggtgtcg ttcgcggtgg agcgcgggga
     4921 agtgttcggg ttcctcgggc cgaacggcgc cggcaagacg acgacgctcg agatcatcga
     4981 gggactgcag tcgccgacgt ccggacgaac cgcggtgctc ggcctggact cccagcagga
     5041 ccggaaccgg atgaaggagc gcatcggcgt gcagctgcag gctggtgcct acttcgacta
     5101 cctgacgctc gaggagatcc tcgagctgtt cggctcgttc tacccgacgg cgctccggcc
     5161 ggacccgctc ctggagaagg tgggcctgct cgagaagcgc aaggcgctgg ttcgggagct
     5221 gtccggcggg caggcccagc gcttctcgat cgtggcctcg atggtgaacg accccgacgt
     5281 gctcttcctc gacgagccga ccaccgggct cgacccccag gcgcggcgca acttgtggga
     5341 gtttatcggg tcgatcaacc gcgacgaggg caagacgatc gtgatgacct cgcactacat
     5401 ggaagaggcg caggtgctct gcgaccgcgt ggcgatcatc gaccacggcg agatccaggc
     5461 actcgacacg cccctcgggc tgatccacac gctgccgacc gcctaccgga tcctgttcgc
     5521 cacgcaggtc ccgatcgacc ccggcccgct ccgcgagctc cctggcgtcg cggaggtggt
     5581 cgccgcacag gacggcgcgt tcgacttcga gctgcgggtg tccagggccc aggtgacgct
     5641 tcccgccttc atcgggtggg ccgagcgggc cggggtccag gtcgacgacg tgcaggtgct
     5701 cccggcaacg ctggaggacg tgttcctctc gctcaccggg cgcagcctga gggagtgagc
     5761 acgatgggga agctcctcgg ggccagcatc aggatgctat accgggaccg gcaggcgctg
     5821 ttctgggcgc tggcgttccc ggtgatcttc gcggtgatct tcggcctctt cgatttcaac
     5881 caggcgcccg aggtcgggat cgccgtcgtc gccgaccggc cgtcgcaggt ctcgcgggcg
     5941 ctcgtcaccg gactgcggga ggtcgagtcg ttcgaggtcg agggacggtc ctctctcgcc
     6001 tccgcgcgaa ccgcgctgga ggacggcgac gtcgacgtgg ccctcgaggt gccggcgata
     6061 cccgcggtac ccgagacgag cacgttcgcg gtgcgcgtgc tctacaaccg gtccaacttc
     6121 aacgtgaacc agttcgccct ctccgcgatc gagcgcatcg ttggcgggct gaacctgcgc
     6181 ctggccggcg tgtcgtcgcc gccgatcgcg gtgcgggccg aggcggtgga cgccaagtcg
     6241 gtcgactact acgacttcct gctcccgggg ctggtggcga tgggcgtgat gaacttctcg
     6301 atcatcggca tcgcggtggg catcacccgc ttccgcgagc agcgcatcct gaagcggatg
     6361 ctggccacgc ctctgcggcc ggcgaagttc ctggcggccc aggtgctggc gcgcctggtg
     6421 ctggccctgg cgcaggctgc cgtgatcctg gcggtcggcg tgttcgtgtt cggggggcgc
     6481 gtctacggca acgtcctctg gatcttcgtg ctggccacgg tggccaacct gatcttcctg
     6541 aacatcggct tcacggtcgc cggacggacc gggaacgtcg acgcggcgca aggcctggcg
     6601 aacttcatcg cgcttccgat gatgttcctc tccggcgtgt tcttcccgac cgacacgctg
     6661 ccgcaggccg tccaggccgt cgtgaagtac ctgccgctga cgccgctgat cgaggcgttg
     6721 cggaaggtgt cggtcgacgg gctctcgatc gtcgacaccg ggccacagct cgtgaagctc
     6781 ggcgcctggg tcgtcgcctc gttcgcgctc gccgctgcgt cgttccgctt cgggcgggag
     6841 gcggggtagc ggctacgcct cccgccaggc ggcgcgcagg ccggggagca cctccgccgc
     6901 cagccgctcg cgctggtcct ccgggtcgag cgccggccag aggttcagcg ccgtgacccc
     6961 cagaccgcgc agcgcggcca ggtgctcgac gaccagcccg ggaggcccgg cgatgctgtt
     7021 cggccgcgcc gtgaccccct cccccaccgc gaccgacacg ttgtagccgc agacgatcgc
     7081 gtcggggtcg cggccggcct tcgcggctgc ccggcgcacc acgtcgcggc gctccgcgta
     7141 gcgctccggc ggcaggtagg gcagggacgg caaccacccg tcgcccagcc gcccggtcac
     7201 ccccagcgcc cgcgtcccgt aggaaccgac ccacacgggg atcgggggct cgggcttcgg
     7261 ctccagctcg gcatcgcgca gcgcgaagta cgcgccgtcg taggtggccg ccggcgccgt
     7321 ccacagcgat cgcatcacct cgatcgactc gcgcagggcg tccaccttct ccgcgggccg
     7381 gcgcaccgcc agcccgagcg cgtggaactc ccgatccgag cctcccccgc ccaccccgag
     7441 caacagccgg ccgccggaga gccgctgcag cgcctcggcc atcttcgcca ggagggcggg
     7501 gtggcgatag ggaaggccga gcacgtcgga gagcaggccg acccgctcgg ttgccgccgc
     7561 cgcccaggtc aggtgggtcc aggtctcgta cgccgggcgg ccgaccaggt gatcggtcac
     7621 cgacaggaag tcgaagccga gggactcgac gtggcgcgcg tcggcgactg gatccatccc
     7681 cggccggacc gcctgccgca gcgcggtccc gaccagcaga gagctcacgg gcgatcggcc
     7741 atcgggacgt ccacgaggag cagctcggtg gtctcctcgg ccgccagggg caggccgcgc
     7801 tcgtcgcgaa ccttcaccgc gtcgccggtg cggagcacct caccgtcctc ccccaggcgc
     7861 agcgctcccc cgatgacgta caggtagccg ccgtggccgg gcgagaacgc gtgcgccacc
     7921 gagacgccgg ggttcaggcg tgcgacgaac accgaggcgt cctggtggac gtggacggcc
     7981 tcaccaccct ccccgccctc ccccgagatc gcccgcagca ggcggtcggt gcggtcctcc
     8041 accgtgaact cgcgctgctc caccgacggc ggcaggccac gctcgtgcgg cagcacccac
     8101 atctggatga agcgcatcgg ctcgctctcg gagccgttct gctcggagtg ccaggcgccg
     8161 ctgccgagcg tcatccgctg caccgagcca gcccgaagca cgccgtcgtt cccgaggtcg
     8221 tcctcgtgcc tgaaggtccc ctcgacaacg taggtcaggc cctcgatatc gcggtgcggg
     8281 tggagcggcc agatcgcgcc ggggatcagc cggtcgtcgt tgaagacgcg cagcaggccc
     8341 cagcccatgt tctccggatc gcggtagcgg tcgaacgaga agtgccagcg ggcctcgaac
     8401 cacccgccct cctcccgatg gatctccccg tcgcgccgga ccgtgatcat cgatgcccaa
     8461 cctaacagcg agcacggata tgatcggcgg ccggaagcgg gggggtcgag cgtgccgaag
     8521 gggtccggga tcgaggtgcg gccggtcacg ccggaccgct gggacgacct cgccgcactg
     8581 ttcggcatcc gcggcgggta cggccactgc tggtgcatgt ggtggcgggt gacgagtgcc
     8641 gagttcgagg gcgggatccg cgaacgcggc cgggggaacc gtcgccggat gcgcacgctg
     8701 gtcagggacg gcgcggagcc gggactgctc gcctaccgcg acgggcggcc ggtgggctgg
     8761 gtgtcggtgg caccgcgcga ccagttcggg cggatcgagc gctcccgggt gctcgggccg
     8821 gtggacgacc ggccggtctg gtccatcgtc tgcttcttca tcggccgggc gcatcgcgcc
     8881 tccggggtag ggacagccct gctcgaggcg gcggtacgcc acgccgcgga ccgcggcgcc
     8941 accgaggtcg aggcctaccc ggtggatccg gcggctcgcg tctactcgaa cgccgacgcc
     9001 tacaccggac tgttgccgat gttcctgcgc gcgggcttcc gtgaggtcga gcggcgcagc
     9061 gccgcgaggc cgatcgtccg caagcgcatc cgccgccggg cgcggcgcgc gaggagcggc
     9121 tgatgcccgg ccgccgccgg accgagccgc gcgaaggcgt ggtgcgccgc ggtggcgacg
     9181 ggcgcgggtt gcgcactcct ggtgacctgt tcctccacgg cggccgacgg cgccgcgagc
     9241 ccggcgaggg ccccggcctt ggcggtcgga cgccccggcc ccgacggcgc ggaccggggt
     9301 gaccgcggcc gccgtgggag acaccgtcta cgtggtgggc ggcttcgtgg aaagcggggc
     9361 gaccgtccgg cgtgtcgagg cgtacgacgg cgcggccggc gcctggtcgg tggcgccgcc
     9421 gctccccatc gccgcgaacc acgccatgtc ggcgggcgcc ggccggcggc tggtggttgc
     9481 cggcggctac cgcggcccga acctctccaa tccgaccgac cgtgcgttcg tcttcctgga
     9541 cgggcgctgg cgggagctgc cgcggatgcc cgagacgcgg gcggccggtg gtgcggccgt
     9601 cgccgccggc cggctctacg tggtcggtgg aacggtcacc gggaccggcg acctcgcccg
     9661 cacgacgctc gtctacacgt tcgccagcgg gcgctggagc acggcgccgg gccttccgac
     9721 ccgccgcgaa cacctcggtg tggccgcctt cggcggcaag gtgtacgcgg tcggtggccg
     9781 gacgggcggg t
//
LOCUS       DASPWO010000288         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1064528, whole genome shotgun sequence.
ACCESSION   DASPWO010000288 DASPWO010000000
VERSION     DASPWO010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064528"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /gene="rpsI"
                     /locus_tag="VEO00_06610"
     CDS             <1..376
                     /gene="rpsI"
                     /locus_tag="VEO00_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HYU57705.1"
                     /translation="YGTGRRKTSVARVWIRPGPGKIVVNRRLFEEYFPRETLRMIIAQ
                     PLQLTNTFGQFDILVRVAGGGPTGQAGAVRHGLSRALVRFDDKLRTTLKKAGLLTRDP
                     RMRERKKYGQPGARQKFQYSKR"
     gene            411..1448
                     /gene="argC"
                     /locus_tag="VEO00_06615"
                     /pseudo
     CDS             411..1448
                     /gene="argC"
                     /locus_tag="VEO00_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
     gene            1448..>2127
                     /locus_tag="VEO00_06620"
     CDS             1448..>2127
                     /locus_tag="VEO00_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HYU57706.1"
                     /translation="MAELEWLDGGITAVPGILASGVAAGIKPSGKKDLALVYSSVPAR
                     AAAVFTSNQVKGAPVLVSMEHVRGGVGQAIVASSGCSNVCTGEEGLRAAREMTRVVGD
                     LLKIPAQRVLVASTGVIGVPFPIDKVRAALPKLVKSLSPKGGRLAAEAIMTTDTRPKE
                     AAVRVEVNGRLVTVGGIAKGVGMIEPHMATMFCFLATDAAVSSAALGSTLKRAVNRSL
                     NRITVDGD"
BASE COUNT          335 a          726 c          754 g          312 t
ORIGIN      
        1 ctacggcacg ggacggcgga agacctcggt ggcgcgcgtg tggatcaggc ccggtcccgg
       61 gaagatcgtc gtgaaccgcc ggctgttcga ggaatacttc ccccgggaaa ccctccggat
      121 gatcatcgcc cagccgttgc aactcacgaa cacgttcgga cagttcgaca tcctggtacg
      181 ggtggccggg ggtggtccca ccgggcaggc gggcgccgtg cgacatggcc tctcccgcgc
      241 gctcgtccgg ttcgacgaca agctgcggac cacgttgaag aaggcgggac tgctgacgcg
      301 cgacccccgc atgcgggagc gcaagaagta cggccagccg ggcgctcgac agaagttcca
      361 gtactcgaag cggtaacgcc acggccaccc acacgttcgt ccgggtcgca gtcgcaggcg
      421 caagcggcta catgggagcc gagctcctgc gcctgctcct cgtccatccg catgtcacgc
      481 ttgcggccgt cacgtccgag cgccacgccg gtgatcgact cgaccaccag tacccgcacc
      541 ttcgcggtct ttcggacctg acgtttcgcg agctcgatcc cgaagacctc gccaccgccg
      601 ccgacgtcgt cttcctcgcg ctcccgcaca tggagtcgca gaaggccgtg ccggtgttgc
      661 gacgccgcgg gcggaagatc atcgatctct cggccgacta ccgactgcgc aacgccgagg
      721 actacacgac gtggtacaag acgccgcacc tggacgccga gggcctggcg gaggcggtct
      781 acgggttgcc cgagctgcac cgcaaggaga tcgcggccgc ggcgctggtg gccgcgccgg
      841 gctgttatcc ggtgggcgcg atcctcgccc tggcgccgct cctgaagtcg gggctgggac
      901 ggaccgacgg cgtggtgatc gacggcaagt ccggcgtcac cggtgcgggc gcgcagagcc
      961 gcaagatcga tccgatgtac ctctacaccg aagccaacga gaacgtgcag gcctacgggc
     1021 tggcgacgca ccggcacacc ccggagatgg agcaggagct gggagcgctc gccgggggcc
     1081 cactgcgcgt caccttcacg ccgcacctcc tgccgttgaa ccgtggcctc ttcaccaccg
     1141 cgacggtgtc gctgacccga gccgtgtcga ccgccgagct ccagacgctg tacggcgagt
     1201 tctacgccgg cgaaccgttc gtccgtgtgt cgcgtgacgg cgacggactg cccgccgtca
     1261 ggcgggtcgt cggctcgaac atgtgcgacg tcgccgtggt ggcgaacccg cggacggagc
     1321 gggcggtctg tgtctcggcc atcgacaacc tcgggaaggg cggatcggcc aacgggatcc
     1381 agaacctcaa cgtgatgttc ggctgggacg agcgcaccgg tctggccgcg ccgccggtgt
     1441 atccgtagtg gccgagctcg agtggctgga cggcgggatc acggccgtcc cgggaattct
     1501 cgcctccggc gtcgccgcag gcatcaagcc cagcgggaag aaggacctgg ccctcgtcta
     1561 ctcctcggtg ccggcgcgcg ccgccgcggt gttcacgagc aaccaggtga agggcgctcc
     1621 cgtcctggtc tcgatggagc acgtccgggg tggggtcggg caggccatcg tcgcctcgag
     1681 cggctgctcg aacgtgtgca ccggcgagga agggctgcgc gcggcgcgcg agatgacccg
     1741 cgtggtcggc gacctcttga agatcccggc ccagcgcgta ctggtggcgt ccacgggcgt
     1801 gatcggcgtc ccgtttccga tcgacaaggt ccgggccgcc ctgcccaagc tcgtgaagtc
     1861 gttgtcgccg aagggagggc ggctcgccgc cgaggcgatc atgaccacgg atacgcggcc
     1921 caaggaagcc gcggtgcggg tcgaggtgaa cggccggctg gtgaccgtcg ggggcatcgc
     1981 caagggcgtg gggatgatcg agccccacat ggcgacgatg ttctgcttcc tcgccaccga
     2041 tgcggcggtg tcgagcgcgg cgctcgggag cacgctgaag cgcgccgtga accggtcgct
     2101 caaccggatc accgtcgacg gcgacca
//
LOCUS       DASPWO010000289         3728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_214322, whole genome shotgun sequence.
ACCESSION   DASPWO010000289 DASPWO010000000
VERSION     DASPWO010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214322"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..882)
                     /locus_tag="VEO00_06625"
     CDS             complement(49..882)
                     /locus_tag="VEO00_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HYU57707.1"
                     /translation="MPEVPEVQALAERLDALLAGAPLDSATTLQFSGLKTYDPPPESL
                     YGATLERVARRGKYVLLNFGGPRIAFHLSQGGRVDVEDPPKTTRPKFAVVRFGFEGRP
                     SILVKEFGTERKAGWWVLADGDDGPLARLGPEPDSPEFERLVLEGTDRRRVHTILRDQ
                     RTVAGIGRGYSDDALHRARLHPFASLSSLSRRERAALLAAVRDTLTEATEVERRRTGG
                     LPTKIGDHFAVHGRYGTPCPRCGQDLRRVSYESCEVTYCPNCQTGGKVLADRRLSRLI
                     R"
     gene            1039..1329
                     /gene="rpsF"
                     /locus_tag="VEO00_06630"
     CDS             1039..1329
                     /gene="rpsF"
                     /locus_tag="VEO00_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HYU57708.1"
                     /translation="MREYEVMFILDPEADESVVSGVADRITGVVSAAGGSISNIDRWG
                     KRRLAYPLDKRTEGYYVVVDFTAGPDSVAELERVLRLADEVVRFKVVRRAAA"
     gene            1400..1837
                     /gene="ssb"
                     /locus_tag="VEO00_06635"
     CDS             1400..1837
                     /gene="ssb"
                     /locus_tag="VEO00_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HYU57709.1"
                     /translation="MATENQIVIVGNLTDDPELRYTPNGAAVTNFSVAVSRRGKDEVT
                     GQWKDLDTSFFRVNVWRATGENVAESLSRGDRVIVVGRLRQRSWETPEGDKRSAIEIE
                     ADEVGPSLKWAKAKPEKQRSGGGDWGENVAVGAGGPAEESPLE"
     gene            1940..2152
                     /gene="rpsR"
                     /locus_tag="VEO00_06640"
                     /pseudo
     CDS             1940..2152
                     /gene="rpsR"
                     /locus_tag="VEO00_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            2163..2609
                     /gene="rplI"
                     /locus_tag="VEO00_06645"
     CDS             2163..2609
                     /gene="rplI"
                     /locus_tag="VEO00_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HYU57710.1"
                     /translation="MKIILQREVGKLGTPGDLVEVADGYARNYLLPRGLAIPASKGAV
                     RHAERLRSVHEDRVRRARVDAEATAGRLGAVIVRITARAGEEGRLFGQVTPERLAEEI
                     TRVTRVPVDRRRIHLEEPIRSLGSHQVSVQVHPEVAATVNVEVVAE"
     gene            2848..>3728
                     /locus_tag="VEO00_06650"
     CDS             2848..>3728
                     /locus_tag="VEO00_06650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HYU57711.1"
                     /translation="MASTIDNVRPLGPERVPPHNPEAEESVLGAMMLSPGAVADVVEE
                     VRADDFYRSAHGRIFQAAIDLYARGEPVDGVTVVEELRRRDQLEGVGGALYLHRLVQA
                     VPTPASARHYAKIVADHALLRRLIDAGSRIIDLAYAVPEHPERAADLAEALVYAVARH
                     GTAEEVVTLGTLVDESIEQLERLHERESAFAGVPTGFTDLDEMLSGLQGGNLIVVAAR
                     PGVGKSSFVTNLARNVAVNASQAVAMFSLEMSRWEIGMRLLCAEARVPWDKVRAARVA
                     AEDWLRIVEAAEVLHEAP"
BASE COUNT          540 a         1289 c         1384 g          515 t
ORIGIN      
        1 tcgctttcgg tggggagcga catccactgc ccctacgtcc cctgcaggtc agcggatgag
       61 gcgggagagc ctccgatcgg ccaggacctt gccgccggtc tggcagttcg ggcagtacgt
      121 gacctcgcag gactcgtacg agacccggcg gaggtcctgg ccgcagcgtg ggcacggcgt
      181 cccgtaacgg ccgtgcacgg cgaagtggtc gccgatcttc gtcggcagcc cgccggtcct
      241 gcggcgttcg acctcggtcg cctccgtcag cgtgtcgcgc acggcggcca gcagcgccgc
      301 gcgctcccgc cgcgacagcg acgagagcga ggcgaacgga tgcagccgcg cgcggtgcag
      361 cgcgtcgtcc gagtatccgc gcccgatccc ggcgaccgtg cgctggtcgc gcaggatggt
      421 gtggacccgg cggcggtcgg tgccctcgag gaccagccgc tcgaactccg gggagtcggg
      481 ctccggcccg agcctggcca gcggcccgtc gtcgccgtcc gccagcaccc accacccggc
      541 cttccgctcg gtcccgaact ccttgaccag gatcgagggg cgcccctcga acccgaaccg
      601 caccacggcg aacttcggcc gggtggtctt cggaggatcc tcgacgtcca cccggccgcc
      661 ctgggagagg tggaaggcga tgcgcgggcc gccgaagttc agcagcacgt acttcccgcg
      721 gcgcgcgacg cgctcgaggg tcgcgccgta cagcgattcg gggggtgggt cgtaggtctt
      781 cagcccggag aactgcagcg tggtggccga gtcgaggggc gcgccggcca ggagcgcgtc
      841 gagccgctcg gcgagcgcct gcacctcggg aacctccggc acgaagccaa ctgtaggcgc
      901 gcaagagcgc tccgggctcg ccgggcgtgt cggtggcggg tgctatggtc gcccggtcgt
      961 ggccctgagg cgactgctat gatcggcgcg tccaccaccc ctgctccttc gggggccgtc
     1021 cagtccacag gaggtgggat gcgggagtac gaggtcatgt tcatcctcga ccccgaggcc
     1081 gacgagtccg tcgtctcggg cgtcgccgat cggatcaccg gcgtcgtgtc cgccgccggc
     1141 ggctcgatct cgaacatcga tcggtggggc aagcggaggc ttgcctaccc cctcgacaag
     1201 cgcaccgagg gctattacgt ggtcgtggac ttcacggccg gcccggactc ggtggccgag
     1261 ctcgagcgcg tgctgcggct ggccgacgag gtggttcggt tcaaggtcgt gcggcgagcc
     1321 gccgcatgac agggacttcc ccccggcgag gggcggccat cggcccccgc cggcagcaac
     1381 gggtgagggg tgaggcctga tggcgacgga gaaccagatc gtgatcgtcg gcaacctgac
     1441 cgacgacccg gagctgcggt acacgccgaa cggcgccgcg gtgacgaact tctcggtggc
     1501 ggtcagccgc cggggcaagg acgaggtgac gggccaatgg aaggacctcg atacgtcgtt
     1561 cttccgcgtc aacgtctggc gcgccaccgg cgagaacgtc gccgagtccc tgtcccgcgg
     1621 cgatcgggtg atcgtggtgg gccgcctgcg gcagcggtcg tgggagaccc ccgagggtga
     1681 caagcggtcg gccatcgaga tcgaggccga cgaggtgggc cccagcctga agtgggcgaa
     1741 ggccaagccg gagaagcagc gctcgggcgg gggcgactgg ggcgagaacg tcgcggtcgg
     1801 cgccggcggc ccggcggagg aatcgcccct cgagtagggg acgtcaggag gcgtttgcgt
     1861 ggcaggacga ggcagagggg gcggtggcaa gccgagcggt aagccgcgcc gcaaggagaa
     1921 ggacgacaag gcctggcaga agaagcagaa gcgcaaggtc tgcgtgttct gccgggacaa
     1981 ggcgacctac gtcgacttca aggacgtcca gctcctgcgc aagttcgtct ccgaccgggg
     2041 caagatccgg gcgcggcggg tctcgggcac ctgctcgcag caccagcgcg acgtggccgc
     2101 ggcggtgaag aacgcgcggg agatggccct ccttccctac accgtcaggt gaggcggggc
     2161 cggtgaagat catcctccag cgcgaggtcg gaaagctcgg gaccccgggc gacctcgtgg
     2221 aggtcgccga cggctacgcg cgcaactacc tcttgccgcg gggcctggcg atcccggcca
     2281 gcaagggcgc cgtgcgccac gccgagcgcc tgcggtcggt gcacgaggac cgcgtccgcc
     2341 gggcccgcgt cgacgccgag gcgacggcgg gcaggctcgg ggcggtgatc gtgcggatca
     2401 cggcgcgggc cggggaggag ggccgcctgt tcggccaggt caccccggag cgcctggcgg
     2461 aggagatcac ccgggtcacg cgggtgccgg tggaccgccg ccggatccac ctcgaggagc
     2521 ccatccggag cctcggctcc caccaggtct cggtccaggt tcaccccgag gtcgcggcca
     2581 ccgtcaacgt cgaggtcgtc gccgagtaga ccccgctccc ctttctgtca tacccatcgg
     2641 gtacgatcga acccgtgttc gatccagcgg gaccagatag tgcgacaagt gcggaacggt
     2701 ttccccagcc cgaacgccta ggtcggaggc ggtttccaca ggcttcccgc ccctcgccca
     2761 caggcctgtg ccccttgttc cccacaggcc gttctctcta cggtgccacg gtcgctggtt
     2821 cgcccgcccc actcgcgaaa gggccccatg gcctcgacga tcgataacgt ccgtccgctc
     2881 gggcccgagc gggtcccgcc gcacaacccg gaggccgagg agtccgtcct cggtgcgatg
     2941 atgctctccc cgggtgcggt ggcggacgtg gtcgaggagg ttcgggccga cgacttctac
     3001 cgctcggccc acggccggat cttccaggcc gcgatcgacc tctacgctcg cggcgagccg
     3061 gtggacgggg ttacggtcgt cgaggagctg cgtcgccgtg accagctgga gggcgtcggc
     3121 ggagccctct acctccaccg gctggtgcag gccgtcccca cgccggccag cgcccggcac
     3181 tacgcgaaga tcgtcgccga ccacgcgctg ctgcgacgct tgatcgacgc cgggtcgcgg
     3241 atcatcgacc tcgcctacgc ggtcccggag cacccggagc gggccgctga cctcgccgag
     3301 gccctggtct acgccgttgc ccggcacggg accgcggagg aggtggtcac cctcggcacg
     3361 ctcgtcgacg agagtatcga gcagctcgag cggctgcacg agcgcgagag cgcgttcgcc
     3421 ggtgtcccga ccggcttcac cgacctcgac gagatgctct ccgggctgca gggcgggaac
     3481 ctgatcgtgg tcgccgcccg ccccggcgtc ggcaagtcct cgttcgtcac gaacctggcc
     3541 cgaaacgtgg ccgtgaacgc cagccaggcc gtggcgatgt tctcgctcga gatgtcgcgc
     3601 tgggagatcg ggatgcgcct gctgtgcgcg gaggcgcgcg tgccctggga caaggtccgc
     3661 gcggcgcggg tggccgccga ggactggctg cgcatcgtcg aggcggccga ggtgctgcac
     3721 gaggcgcc
//
LOCUS       DASPWO010000290         4231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1228820, whole genome shotgun sequence.
ACCESSION   DASPWO010000290 DASPWO010000000
VERSION     DASPWO010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228820"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1746
                     /locus_tag="VEO00_06655"
     CDS             82..1746
                     /locus_tag="VEO00_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57712.1"
                     /translation="MAAQIGTEPPSTDEARELARRLGARFYVQGSVSYSGSAVTLTAT
                     LYEGGRGRRTGEATGRVGVDESAVMDRVWAQLYPEFRPSTDVTLPNGGPEALAAYLNA
                     EAAFRRGDYRTARDEYTRVIRADTSFAIGRLRLALVAAQVDPTEQGFGAALRGAELHQ
                     QGLSGADSLLLEGFSMLLLRGDGLAALERFKRATESAPAYPQAWYVLGEFYYHFGGLF
                     DQSIGEARVAFDRVLDLDPRFSPAIAHLISLANQAGDRGETESLIRRYLRIDSTSVVA
                     EAVGIADTVILGTPAAQLALLKTVCRHSFLALQYLAFQAAAFGTPAQREGPGRTVLRC
                     LDARGATDAERVLALRMGVAADLATGRPDSARRRLDRATAAWAGAGRERAVWVLVAHA
                     AGLPALGDWRRAAAELATRPRAVADTDVVAHWALARAAVDRPRHAAALARLAAGGAPP
                     PLPASLAADLQAHAALARGDSAAALRLWDGATRRYAVLSVTFDPVASLWPLRLDVART
                     AVARHDSAAAARACESFDMLIGYADQVARPEIERLCRGRGATRSPP"
     gene            complement(1822..2958)
                     /locus_tag="VEO00_06660"
     CDS             complement(1822..2958)
                     /locus_tag="VEO00_06660"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYU57713.1"
                     /translation="MHRRAAVPVALSAAALLFVACSDSSPLAPAPMTLFRGDGAGDLF
                     APGVTALTWNVYVGAEIDRVLEARTPEEAVLLATEQWANVQATDFPARAGAIAAGIAA
                     RRPQLVGLQEVALYRTTDRPFEELATHVAYDFLQLVIDSLHARGLDYVAAAVDRTTDL
                     QVPVIARFDASGQPVFAGVRFTDGDAVLLRADVPHANARSGVYGAYVPVTIGGVTSGV
                     YQGWSSVEATIGGATYRFVVTHLAGQEVAELQVAQTRELVALLADDSRPTVLVGDFNS
                     DAYGADPARATPTYGMLGAAGYADSWIEPDRRAPGLTCCERPDLLNPVPAFDQRIDFI
                     FTRNLPAGAPALGRDVVGDRPGDRTAGLWPSDHAGVVATFRAPR"
     gene            complement(3030..4136)
                     /locus_tag="VEO00_06665"
     CDS             complement(3030..4136)
                     /locus_tag="VEO00_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HYU57714.1"
                     /translation="MTRHAGIFALFVVAACDASQRVAAPPALSAERFGRPDVVVPAGG
                     SIQAAVDAVSPGAVIAIEPGVYTEAVHVAVPGLTLVGRRGGGGGGVVIENPGDEENGI
                     AVAPGSDGFTLANVTVRGFEENGVLLVGVNGFRLSEITAEDNGEYGLFPVHSSHGVIE
                     GCRASGHDDTGIYVGQSHDVTVRRSVAFANVNGIEFENSQRVRALGNETYDNVVGILV
                     VLLPGLDVKTTSDVLVSGNRVHDNNHPNFAPPGDLASFVPSGSGILVVGADRVKVEGN
                     TVTGNQFVGVGMASTLVLGQLAGLPPDAFGDIEPDPDSNRVVDNVVTGNGGPPSPIPG
                     LPAVDLLWVPAGTGDCWSGNTFGTSFPEQLPTCR"
BASE COUNT          574 a         1556 c         1538 g          563 t
ORIGIN      
        1 cccgcgacgc agctcccgga atacttcgtc tcacggttcc ggcccgtgga acgggtgatc
       61 gacgtcgtgt cgttcggccg ggtggccgcc cagatcggca ccgagccgcc atcgaccgac
      121 gaggcgcgcg agctggcgcg ccgcctgggc gcgcgtttct acgtgcaggg gagcgtgtcg
      181 tattccggat cggcggtgac gctgacggcg acgctgtacg agggcggtcg cgggcgccgc
      241 accggcgaag caaccggccg cgtcggcgtg gacgagtcgg cggtgatgga ccgcgtgtgg
      301 gcccagctgt acccggaatt ccggccgagc accgacgtca cactgccgaa cggcggtccc
      361 gaggcgctcg ccgcctacct caacgccgaa gcggccttcc ggcgcggcga ctaccggacc
      421 gcgcgggacg aatacacgcg cgtgatccgc gccgacacga gcttcgccat cgggcgcctg
      481 cgcctcgcgc tggtcgccgc ccaggtggac cccacggagc agggcttcgg tgcggcgctg
      541 cgtggcgccg agctgcatca gcagggtctg tccggcgccg acagcctgct gctcgagggg
      601 ttctcaatgc tgctgctccg cggcgacggg ctcgcggcgc tcgagcggtt caagcgcgcc
      661 accgagtcgg cgccggccta cccccaggca tggtacgtgc tcggcgagtt ctattatcac
      721 ttcggcggcc tgttcgatca gagcatcggg gaggcgcggg tcgccttcga ccgggtgctc
      781 gacctcgatc cccggttctc cccggcgatc gcgcacctga tttcgctcgc gaatcaggcg
      841 ggcgaccggg gcgaaacgga gagcctgatc cggcgatatc tgcggatcga ctcgacctcc
      901 gtcgtcgccg aggccgtcgg catcgcggac acggtcatcc tcggcacgcc cgccgcgcag
      961 ctggccctcc tcaagacggt gtgccggcac agctttctcg cgctccagta tctggcgttc
     1021 caggccgcgg ccttcgggac gccggcgcaa cgcgaaggcc cggggcgcac cgtgctgcgc
     1081 tgcctcgacg cgcgcggcgc gacggatgca gaacgggtgc tggcgctacg gatgggggtc
     1141 gcggccgatc tcgcgaccgg acggccggac agcgcgcgcc gccggctcga ccgcgcgacc
     1201 gccgcctggg cgggggcggg gcgggagcgc gcggtgtggg tgctcgtggc gcacgccgcg
     1261 ggcctgccgg cgctcggcga ttggcggcgc gcggcggcgg agctcgcgac ccggccgcgc
     1321 gccgtggcgg atacggacgt ggtggcgcat tgggcgctcg cgcgcgccgc cgtggaccgg
     1381 ccgcgccacg ccgcggcgct cgcgcgtctc gccgcgggcg gcgctccccc cccgctgccg
     1441 gcgagcctcg cggccgacct ccaggcccac gccgcgctcg cccggggcga ttcggccgcg
     1501 gcgctccggc tctgggacgg ggcaacgcgc cgctacgccg tgctcagcgt gacgttcgac
     1561 ccggtcgcgt cgctgtggcc gctccgactc gacgtggcgc gcacggcggt ggcgcggcac
     1621 gacagcgccg ccgcggcgcg tgcctgcgag tcgtttgata tgctgatcgg ctacgccgac
     1681 caggtcgccc ggccggagat cgagcgtctg tgccgagggc gcggcgccac gcgatcccca
     1741 ccctagaagg gtgggctcgg cgagtcgctg cccttaaagg gcagtgccgt gccgagccca
     1801 ggcttcagcc tgggaagggc ttcaacgcgg cgcgcggaag gtcgccacca cgccggcgtg
     1861 atccgagggc cacagcccgg ccgtccggtc ccccggccgg tcgcccacga cgtcgcgccc
     1921 cagggccggc gcgcccgccg gcaggttccg cgtgaagatg aaatcgatcc gctggtcgaa
     1981 cgccggcacc gggttcaaca ggtcgggccg ctcgcagcag gtgaggccag gtgcccggcg
     2041 gtccggctcg atccagctgt ccgcgtatcc ggccgcgccg agcatgccgt acgtcggcgt
     2101 cgcgcgcgcc ggatcggcgc cgtaggcgtc cgagttgaag tcgcccacga gcacggtggg
     2161 ccgggagtcg tccgcgagga gcgccacgag ctcccgggtc tgcgcgacct gaagctccgc
     2221 gacctcctgg ccggcgaggt gggtgacgac gaagcggtag gtcgcgccgc cgatcgtcgc
     2281 ctccacggag ctccagccct ggtacacgcc cgaggtcacg ccaccgatcg tcacgggaac
     2341 gtaggcgccg tacacgcccg accgcgcgtt ggcgtgcggc acgtcggcgc gcaacagcac
     2401 cgcgtcgccg tcggtgaagc gcacccccgc gaagaccggc tggcccgacg cgtcgaaccg
     2461 cgcgatgacc ggcacctgca ggtcggtcgt gcggtccaca gcggcggcca cgtagtcgag
     2521 accgcgtgcg tggagcgaat cgatcacgag ctggagaaaa tcgtacgcga cgtgcgtcgc
     2581 gagctcctcg aacggccggt ccgtcgtccg gtagagcgcc acttcctgga gccccacgag
     2641 ctgcggccgc cgggcggcga tgcccgcggc gatggcgccc gcgcgcgctg gaaagtccgt
     2701 cgcctggacg ttcgcccact gttcggtcgc gagcagcacg gcctcctcgg gcgtgcgggc
     2761 ctcgaggacg cggtcgatct cggcgccgac gtacacgttc caggtgagcg ccgtgacgcc
     2821 cggcgcgaac aggtcgcccg ccccgtcgcc ccggaacagc gtcatcggag cgggagcgag
     2881 cgggctcgag tcggagcatg cgacgaagag cagggcggcg gcggacaggg cgacgggcac
     2941 ggcggcacgg cgatgcatgt gaccctcctt ggggaccggc gacgagattc gcgcggtccc
     3001 gtcaagaagg ggtcaagcgc cgccggggtt cagcggcagg tcggcagctg ctcggggaag
     3061 ctcgtcccga acgtgttccc gctccagcaa tcgcccgtcc cggccgggac ccacaggaga
     3121 tccacagcgg gcaggcccgg gatgggcgac ggcggtcccc cgttgccggt gacgacgttg
     3181 tccacgaccc ggttcgagtc cgggtcgggc tcgatgtcgc cgaaggcgtc cggcggcagc
     3241 cccgcgagct ggcccagcac cagcgtggac gccattccaa cgccgacgaa ctgattgccc
     3301 gtcaccgtgt tgccctcgac cttcacccgg tcggcgccga cgacgaggat ccccgagccg
     3361 ctcggcacga acgaggcgag atcgcccggc ggcgcgaagt tcgggtggtt gttgtcatgc
     3421 acccggttcc cggacacgag cacgtcactc gtcgtcttca cgtcgagccc cggcaggagc
     3481 actacgagga tcccgactac gttgtcgtag gtctcgttgc ccagggcgcg cacacgctgc
     3541 gagttctcga attcgatgcc gttgacgttg gcgaacgcga cgctgcgccg gaccgtgacg
     3601 tcgtgcgatt gcccgacgta gatcccggtg tcgtcgtgtc ccgacgcgcg gcatccctcg
     3661 atcaccccgt gcgacgaatg cacggggaac agcccgtact ccccgttgtc ctcagcggtg
     3721 atctccgaca gccggaagcc gttcacgccg accaggagga cgccgttctc ctcgaacccc
     3781 cggacggtga cgttcgcgag cgtgaacccg tcgctccccg gcgccacggc aatgccgttc
     3841 tcctcgtcac cggggttctc gatcacgacg ccccccccgc cgccgccgcg gcggccgacg
     3901 agcgtgagcc cgggcaccgc gacgtgcacc gcttcagtgt agacgcccgg ctcgatcgcg
     3961 atcacggcac ccgggctcac cgcgtcgacg gcggcctgga tcgacccgcc ggcgggcacg
     4021 acgacgtcgg gccgcccaaa ccgctcggcg gacagcgccg gcggcgccgc gacccgttgg
     4081 gacgcgtcgc acgcggcgac gacgaatagc gcgaagatcc ccgcatgacg agtcatacga
     4141 ccctccccgg ttgcgatcac gccgaacggc cggcggccac gaggatgcgc tggcgctgcc
     4201 ccggcgtgac catgtcgcgc tccaccacgt c
//
LOCUS       DASPWO010000291        13064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_811922, whole genome shotgun sequence.
ACCESSION   DASPWO010000291 DASPWO010000000
VERSION     DASPWO010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13064
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811922"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..378)
                     /locus_tag="VEO00_06670"
     CDS             complement(55..378)
                     /locus_tag="VEO00_06670"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HYU57715.1"
                     /translation="MDEVKWASGLWHVREGKADEFVERWTAWITWTGENIPGFRSSRL
                     LRAGGDPLRFVSVSDWDDDASLKAWKASPGFREKIESVKELCDEFLGGGYDVAAAVLS
                     PALHA"
     gene            complement(491..2488)
                     /locus_tag="VEO00_06675"
     CDS             complement(491..2488)
                     /locus_tag="VEO00_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HYU57716.1"
                     /translation="MTPSASFRIEMFGGLRVFVEDRAVERSAWSRRSARGVVSLLALA
                     SGHRLNREQVMFALWPELEPEAAAANLRKAVHLVRRALDADPERARSLLIGDAETVSL
                     PPDVWTDVGAFRSAAASARRSSDPSEYATALDLYAGDLLPEDRYEEWVAPTADELRSE
                     WGALLSEQAGLLEARGELDEAAAALRRALVAEPLEEDLVVRLMRVLALTGRRHEAADI
                     YDRFSAALQAEMGVEPSLPTQRLRQEIAAGERLEPEVAAALWARIGELRMRAGDLVGA
                     ADAFESAISLGAPGPEALPNLHIGAARAHLGAHEATRAERHVDAAESLLPGDRGAKSR
                     IATLRANVAWERGDLTQADRLAREAIELSEGGDPDDVAAANEAFAIVCHFRGAWPGGL
                     TEEIDRLGRLPDGDSALAGVFDIHHCIGQYHLYGDGLWEHVEDYARETLDRAGRLGAV
                     RAQAFAWCLLGESLLLQARYEEAEGCLERSGELHASLGERSGALAWQRLAELLVCRGE
                     QDAAAQPLRRASAIATVSPMAPHLWGRIHATHALAALLRDDPGEAVRHVQSAAKAAAR
                     YGDCPTCSALLNPLAAEAYALLGDAAGARSHAEAAERVAAMFGSSAWRAMAETATGAA
                     KAAEGDRQAASAAFEVAAEHYERAGQPYWAARTRDRARAFA"
     gene            2780..5143
                     /gene="lon"
                     /locus_tag="VEO00_06680"
     CDS             2780..5143
                     /gene="lon"
                     /locus_tag="VEO00_06680"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HYU57717.1"
                     /translation="MPEQLDTRILPLLPLTSGVVLPGMVITLMLETGEAKAAATAAYE
                     QGDLLVLVPRSGNRYAAVGTVAKIEDSGQLANGMEALVIRGLHRAVVGTGVAGTGDAT
                     WVQIEDAPETNTNTTRARELAREYRGTVENIVEARGVPQVAEVLRGITDPGALADMAG
                     YSPDLSLEQKIEVLEALDVETRLEKVLNWAKDTLAEVTVKDKIREEVQDNLDKRQREF
                     ILRQQMEAIRKELGEDDEENVVEEYRRRIDEANMPEDVRKQAERELGRLERMSEQSPE
                     QGWIRTYLDWLLDVPWDVRTEDNLDIADARRILDEDHTGLEDVKDRIVEYLAVRKLRR
                     ERGLAEQTQGRGSGAILTLIGPPGVGKTSLGESVARALGRKFVRVALGGIRDEAEIRG
                     HRRTYVGAMPGRIVRALKDAGTKNPVMMLDEIDKVGADWRGDPSSALLEVLDPAQNHT
                     FRDHYLDVDLDLSEVLFIATGNVAETIPGPLYDRMEVIRLDGYTEDEKVAIARDHLLA
                     RQVEKNGLQPDEVVVTDGALRAVVGDYTREAGVRNLERELGKAMRKVATKVAAGDVTP
                     PVTIDSGDVREYLGRQRFFFEAAERTSVPGVATGLAVTGHGGDVLFIEATRMDANEGS
                     DGLTLTGQLGDVMKESAQIALSYVRSHAEELGISPQDLKARFHVHVPAGAVPKDGPSA
                     GVTMTTALASLLTGRNVKPTVGMTGEVTLQGRVLPIGGVKQKVLAAHRAGLKEVILPL
                     RNEGDLDDVPEQVRNEIEFHVAESIDQVLDWALEPETEPRLEPVPAA"
     gene            complement(5223..6389)
                     /locus_tag="VEO00_06685"
     CDS             complement(5223..6389)
                     /locus_tag="VEO00_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57718.1"
                     /translation="MRNWSSRGILALTALLVIVWSNIAVASNGDPLTAGADTSATRQT
                     SLTTTQSSGSGLLVTLDGTGTGSAVRGRSTGAGNGVLGTSVTGFGVKATTDSGSQASL
                     VANNSGGGPAASFTVASGAPFTVSSGTVVTNLNADRLDGKNASAFLPATGKAVDADKL
                     DGHDSSEFIPNTVPLSLTGVAAGAGVVNGTNTGSGNGLQGVTAAAGASGVYGQNDAAG
                     FGVAGRADAAGGVGVYAESTGGGPALQIHTLSNAPPMSVDSSVKVANLNADRVDGASI
                     LSNRIISTTHNDHILQLPGFGDFNVSSCDHTNAAFEWSSGGPQAYVIWADVFNPGDSF
                     VGVANIVFSFARPHHFATVQLARDVGANTSLATVTITTNGADCVFAAQAIVQPA"
     gene            complement(6753..6896)
                     /locus_tag="VEO00_06690"
     CDS             complement(6753..6896)
                     /locus_tag="VEO00_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57719.1"
                     /translation="MTTKTKSAAAKSSPSAAKVVYKTSRRYGRLTAKQVKEAVAAVSR
                     KSS"
     gene            6971..7372
                     /locus_tag="VEO00_06695"
     CDS             6971..7372
                     /locus_tag="VEO00_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005483264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU57720.1"
                     /translation="MTERIPVIELRVALTVEDLDAAVAFYRDALGLPVELSWDTPDGR
                     GFVLQAGQATLELIDAAQADTIDRVEVGRRTAGPVRLALEVGDSEATAGRLVDAGAEL
                     LGGPVETPWGDRNARVRAPDGMQLTLFTRSG"
     gene            7442..7957
                     /locus_tag="VEO00_06700"
     CDS             7442..7957
                     /locus_tag="VEO00_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57721.1"
                     /translation="MGGRSRGAPARRLRAVGAGLVVLMLAIAGCRSARQAEAAHGPCP
                     AGDPLRGVYHPDRLRVLGTCVTYRGVVRDFKHESDGDYHLYVIPALGMDHFLDPRSEG
                     YGDLVVELLPGQALPLPQPGQRVTLVGTWAYDTDHDWNELHPVWAETIRGRIHTGLPP
                     VPPEYGGSSNA"
     gene            complement(8061..8513)
                     /locus_tag="VEO00_06705"
     CDS             complement(8061..8513)
                     /locus_tag="VEO00_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57722.1"
                     /translation="MGKVRNALSTLGITLQTPEHWNKWWILMWFVTGPGLIVLAFFIP
                     FWAWVPAALVGFLLPEIISLVKEKDSLPPLTHTIRHYLKDFLAFPIIYFAVGAVGAHW
                     LDFRRWWAVGGLFALLGWLTDHFSVTYAHPDPFPFTGGPRVEPQRRPL"
     gene            complement(8711..9100)
                     /locus_tag="VEO00_06710"
     CDS             complement(8711..9100)
                     /locus_tag="VEO00_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57723.1"
                     /translation="MRANRIVRLGIVGMIVGAMFAAAPAAMAKGGVTTRGACSGAADW
                     KLKVSPEDGGMLEVQFRVEDAKPGQEWTVQLADNGTQIFSGSRTANDLGKFKVRTMTA
                     DRAGSDTVTAQATNTVTGQTCNGSATL"
     gene            9278..9583
                     /locus_tag="VEO00_06715"
     CDS             9278..9583
                     /locus_tag="VEO00_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014695819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57724.1"
                     /translation="MRTRRALVALSVAAVVGLAPTAAFAKHGADDPKGRHVREDRATV
                     TRVMDTRGKDDPAGDDKGRHAEPEPGDDRGGAARHAEPGDDHGRHGGGEPGDDHGGR"
     gene            9772..11187
                     /locus_tag="VEO00_06720"
     CDS             9772..11187
                     /locus_tag="VEO00_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU57725.1"
                     /translation="MAQILHRPRAHARVSDDEEGSSGTRRGRGAAAMRAQARRTIGRS
                     VAVFAVSALAAVVIVAVIESFVFRSAGTAEAIRDARQVSVTVGTGMVEPALRDGIFTG
                     DPAALNSLDRVVTGRVLSRSIVRVNIWDASGRILYSDEHRLIGASYRLGDDESAALHG
                     DRPEADLSDLSAPENRFERPFGSLLEVYMPVHTPNGTPLLFESYHRYSAVAASGRKVW
                     LGVVPALLIGLAVLALVQVPLAWRMARGLERGRLDRERLLRRAVEASTAERRRIAGDL
                     HDGVVQDFAGLSMSLAAAGERAEADGAPAAAAALREGAGRTRQGMRQLRTLLVEIYPP
                     NLHTAGLEPALSDLLAPLAARAILTHLEVAPGVHALQADRAIEGLVFRTAQEALRNVV
                     AHAEAASVEVRLATGHGMLELTVRDDGRGFSTADLDRSGEEGHMGLPLLAGLAEEAGG
                     SLEVDSEPGHGTSVRLEVPAA"
     gene            11184..11882
                     /locus_tag="VEO00_06725"
     CDS             11184..11882
                     /locus_tag="VEO00_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015036686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU57726.1"
                     /translation="MTNTNANSTSKSNTPEDATDVSRSIRVMVVDDHAVVRAGLEQLL
                     ASFPDVEVVGSAGGGAEAVELSAEHEPDVVLMDLSMPEVDGIEATRRIAERTPATRVV
                     IFTSFSERDRILSAIDAGAIGYLLKDAEPEEIHRGLQAAARGESPLAAKAAQAVVAAG
                     IESRPVRELTDREREVLVLVATGLANKQIARRLGITEKTVKAHLTRLFQRLGVTDRTQ
                     AALWAERHGLLAAS"
     gene            12003..12248
                     /locus_tag="VEO00_06730"
     CDS             12003..12248
                     /locus_tag="VEO00_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57727.1"
                     /translation="MTVPMTISDGSGGDTVQGGSGADVFVPGTSGAEADSYAGGAGAD
                     HLTGSGGPNILAGAGGDAVLKGLGGNDTLLGGPGTTR"
     gene            12418..12840
                     /gene="msrB"
                     /locus_tag="VEO00_06735"
     CDS             12418..12840
                     /gene="msrB"
                     /locus_tag="VEO00_06735"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HYU57728.1"
                     /translation="MTAKVQKSEAEWREQLSPEQFHVLREKGTERAFTGAYWDEHADG
                     VYRCAACGAELFRSDEKFDSGTGWPSFWDPATSDAVELHHDFSHFMHRVEVTCATCGS
                     HLGHVFDDGPRPTGKRYCINSCSLELETTTAAGPSNPA"
BASE COUNT         1899 a         4698 c         4663 g         1804 t
ORIGIN      
        1 ctcatctgac tcctcctggt tcgtggggtc gtgcgtgatc gtcggtggga gagctcaagc
       61 gtgcagtgcc ggcgacagga cggctgccgc gacgtcgtag cccccgccca ggaactcgtc
      121 gcacagctcc ttcaccgatt cgatcttctc ccggaacccc gggctggcct tccacgcctt
      181 cagcgaggcg tcgtcgtccc agtccgagac cgagacgaac cgcaggggat cccccccggc
      241 ccgcaggagc cgggacgacc ggaaccccgg gatgttctcg ccggtccacg tgatccacgc
      301 ggtccagcgt tcgacgaact cgtccgcctt cccctcccgc acgtgccaca ggccggaggc
      361 ccacttgacc tcgtccatcg ccggctcctt ccgcgtccgg gcacctcgat cgggtgccga
      421 tgggcgaaat ctagggaggg ggcgttccct cagcgttccc tctgtccggg cttcggctga
      481 ggctcttcga tcaggcgaac gcccgggccc gatcccgagt ccgggcggcc cagtacggct
      541 gcccggcccg ttcgtagtgt tcggccgcga cctcgaaggc ggcgctcgcg gcctgccggt
      601 cgccctcggc ggccttggcc gctccggtgg ccgtctcggc catggcccgc caggcggagc
      661 tcccgaacat cgccgcgacc ctctcggccg cctcggcgtg cgagcgggcc cccgcggcat
      721 cgccgagcag cgcgtacgcc tcggcggcga gcgggttcag cagggcagag caggtggggc
      781 agtcgccgta ccgggctgcc gccttcgcgg ccgactgcac gtggcgaacg gcctcgccgg
      841 ggtcgtcccg taagagcgcc gcgagggcgt gcgtggcgtg gatccgtccc cacagatgcg
      901 gcgccatggg cgacacggtg gcgatcgccg acgcgcggcg aagcggctgg gcggcggcgt
      961 cctgttcccc tctgcagacg agcagctctg ccagtcgctg ccaggccagg gcgcccgagc
     1021 gttcgcccag cgaggcgtgc agctcgccgc tccgctcgag gcacccctcg gcctcctcgt
     1081 accgggcctg gagcagcagc gactcgccca gcaggcacca cgcgaacgcc tgcgctcgga
     1141 cggccccgag cctccccgcg cggtcgagcg tctcccgcgc gtagtcctcg acgtgctccc
     1201 acaggccgtc gccgtacagg tggtactggc cgatgcagtg gtggatgtcg aacacgccgg
     1261 cgagggccga gtccccgtcg ggcaggcgcc cgagccggtc gatctcctcc gtcagcccac
     1321 ccggccacgc gccccggaag tggcagacga tggcgaacgc ctcgttggcg gccgccacgt
     1381 cgtcgggatc gccgccctcg gacagctcga tcgcctcgcg ggcgagccga tcggcctgcg
     1441 tgaggtcgcc tcgttcccag gccacgttcg cccggagcgt ggcgatccgc gacttcgcgc
     1501 cccggtcgcc gggcagcagc gactcggcgg cgtcgacgtg ccgctcggcc cgggtggcct
     1561 cgtgcgcccc gaggtgggcc ctcgcggcgc cgatgtgcag gttcggcagg gcctcggggc
     1621 cgggcgcccc gagcgagatc gccgattcga acgcgtcggc cgcgccgacg aggtcaccgg
     1681 cgcgcattcg aagctcgccg atccgcgccc acagcgcggc ggccacctcg ggctcgagcc
     1741 gctcgcccgc agcgatctcc tgccggagcc gctgggtggg gagcgagggt tcgactccca
     1801 tctcggcctg gagggctgcg ctgaaccggt cgtagatgtc tgctgcctcg tgccgccgac
     1861 cggtcagcgc caggacacgc atgagccgga cgacgaggtc ctcctccaac ggctccgcga
     1921 ccagcgcccg acgcagcgcc gcggccgcct cgtcgagctc gccacgcgcc tcgagcagcc
     1981 ccgcctgctc ggacagcagc gccccccact cggaccgaag ctcgtcggcg gtgggagcga
     2041 cccactcctc gtaccggtcc tcgggaagaa ggtcgcccgc gtagagatcg agggccgtgg
     2101 cgtattcgga cggatccgag gaacgccgcg ccgaggcggc cgcggagcga aacgcaccca
     2161 catcggtcca gacatcgggc gggagcgaga ccgtctctgc gtccccgatc agcaaggaac
     2221 gagcccgttc ggggtccgca tcgagcgccc gccgaacaag gtgcacggcc ttccgcaggt
     2281 tggcggccgc cgcttcgggt tcgagctcgg gccacagggc gaacatgacc tgctcccggt
     2341 tcagccgatg cccggacgca agagccagca gcgagacgac ccccctcgcg ctccgccggc
     2401 tccacgccga ccgctcgacc gctcgatcct ccacgaagac gcgcagcccc ccgaacatct
     2461 cgatccgaaa tgacgcgctc ggtgtcatcg gagcggatgg taggcgacga cgggatgcag
     2521 acgtggcccg agatgcagca ggggagtctc catacacgtg cttggccagc tccgggccgg
     2581 cgcttgtctt cgtgttcgcg tccggcttcc tttccggggt aaggcttgcc gacatcgcgg
     2641 tggcccgtgg gcgtctgcaa atgcggggtg cacgtagacg accgcacgct cagggttgag
     2701 tctggggggc tcaagtctgg gaatacacgg tagctcgctg cacgtttcac acgtcgacgg
     2761 atctcatgag gagttcgacg tgcctgaaca gcttgataca cggatccttc ccttgctgcc
     2821 gctgacctct ggggtcgtgc tgccggggat ggtcatcaca ctcatgctcg agacgggcga
     2881 ggccaaggcc gcggccaccg cggcttatga gcagggcgac ctgctcgtgc tcgttccgag
     2941 gagcgggaac cgctatgccg cggtcgggac cgtggccaag atcgaggact ccggccagct
     3001 ggccaacggc atggaggccc tggttatccg cggtctgcac cgggccgtcg tcgggaccgg
     3061 cgtggccgga accggggacg ccacctgggt gcagatcgag gacgcgcccg agaccaacac
     3121 caacacgacc cgcgcccgag agctcgcacg cgagtaccgg ggcacggtcg agaacatcgt
     3181 cgaggccagg ggagttcctc aggtcgccga ggtgctccga ggcatcacgg acccgggcgc
     3241 tctggccgac atggccgggt actcgccgga cctttcgctc gaacagaaga tcgaggtgct
     3301 cgaggcgctc gacgtcgaaa cccggctcga gaaggtcctg aactgggcca aggacaccct
     3361 cgccgaggtc accgtcaagg acaagatccg cgaggaggtc caggacaacc tcgacaagcg
     3421 gcagcgggag ttcatcctgc gccagcagat ggaggccatc cgcaaggagc tcggggagga
     3481 cgacgaggag aacgtcgtcg aggaatacag aaggcggatc gacgaggcga acatgcccga
     3541 ggacgttcgc aagcaggcgg agcgggagct cggccggctg gagcgaatgt cggagcagtc
     3601 gcccgagcag gggtggatcc ggacctacct ggactggctg ctcgacgtgc cgtgggacgt
     3661 tcggaccgag gacaacctgg acatcgccga cgcccggcgg atcctcgacg aggaccacac
     3721 cgggctcgag gacgtgaagg accggatcgt cgagtacctc gccgtgcgga agctccgccg
     3781 ggagcgcggg ctggccgagc agacccaggg ccgcggctcg ggcgcgatcc tcacgctgat
     3841 cgggcctccc ggcgtcggga agacctccct gggcgagtcc gtcgcccgcg cgctcggacg
     3901 gaagttcgtt cgggtcgccc tcggcggcat ccgggacgag gccgagatcc gcgggcaccg
     3961 ccggacctac gtcggcgcca tgccgggccg cattgtccgc gccctgaagg acgccggcac
     4021 gaagaacccg gtgatgatgc tcgacgagat cgacaaggtc ggcgcggact ggcggggcga
     4081 cccgtcctcg gcgctgctcg aggtcctgga cccggcgcag aaccacacgt tccgcgacca
     4141 ctacctggac gtcgacctcg acctgtcgga ggtcctgttc atcgccaccg gcaacgtcgc
     4201 cgagaccatc cccgggccgc tctacgaccg gatggaggtc atccgcctcg acggctacac
     4261 cgaggacgag aaggtcgcca tcgcccggga ccacctcctg gcccggcagg tcgagaagaa
     4321 cggcctccag cccgacgagg tcgtcgtgac cgatggcgcc ctgcgcgcgg tggtcggcga
     4381 ctacacccgg gaagccggcg tccggaacct cgaacgggag ctcgggaagg ccatgcgcaa
     4441 ggtcgccacg aaggtcgctg ccggggacgt cacgcccccg gtgacgatcg actccggcga
     4501 cgtccgcgag tacctgggcc ggcagaggtt cttcttcgag gcggccgagc ggacctcggt
     4561 gcccggggtc gccaccggcc tggccgtcac cggccacggc ggcgacgtcc tgttcatcga
     4621 ggccacccgg atggacgcga acgagggctc cgacggcctc accctcaccg ggcagctcgg
     4681 cgacgtgatg aaggagtcgg cgcagatcgc gctgtcctac gtccgctcgc acgccgagga
     4741 gctggggatc tcgccccagg acctcaaggc ccggttccac gtccacgtcc ccgcgggcgc
     4801 ggtgccgaag gacgggccgt cggccggcgt gaccatgacc acggcgctcg cgtccctgct
     4861 caccgggcgg aacgtgaagc cgaccgtcgg catgaccggc gaggtcaccc tccagggcag
     4921 ggtcctgccc atcggcggcg tgaagcagaa ggtcctcgcc gcccaccggg ccgggctgaa
     4981 ggaggtcatc ctcccgctgc gcaacgaggg cgatctcgac gacgtccccg aacaggtccg
     5041 caacgagatc gagttccacg tcgcggagtc gatcgaccag gtcctcgact gggcgctgga
     5101 gcccgagacc gaaccccgcc tggagccggt cccggccgcg taaaggtcaa agacctgccc
     5161 gaaaccgaaa caggaacgag cccgggtctc ccgcccgggc tcgttcctgt cggagctcgt
     5221 tcctatgcgg gctggacgat cgcctgggcg gcgaacacgc agtcggcccc gttcgtcgtg
     5281 atggtcaccg tcgcgaggct cgtgttcgcg cccacgtccc gggcgagctg aacggtcgcg
     5341 aagtggtgcg gccgggcgaa cgagaagacg atgttcgcga cacccacgaa ggaatcacca
     5401 ggattgaaca cgtcggccca gatgacgtac gcctggggcc cgccggagga ccactcgaac
     5461 gcggcgttcg tgtggtcgca cgacgagacg ttgaagtcgc cgaagcccgg cagctgcagg
     5521 atgtggtcgt tgtgcgtcgt gctgatgatc cggttcgaca ggatcgaggc gccgtccacc
     5581 ctgtcggcgt tcaggttcgc caccttcacc gacgagtcga cgctcatcgg cggcgcgttg
     5641 ctgagcgtgt ggatctggag tgcgggcccg ccgcccgtcg actccgcata caccccgacc
     5701 ccgcccgctg cgtccgcccg gccggccacg ccgaagccgg ccgcatcgtt ctggccgtag
     5761 acgccgctgg cgccggccgc ggcggtgacg ccctgaagcc cgtttccgga accggtgttc
     5821 gtgccgttca cgacgcctgc gcccgccgca acgccggtga gcgagagcgg cacggtgttc
     5881 gggatgaact cgctcgaatc gtgaccgtcg agcttgtcgg catcgacggc cttgccggtc
     5941 gcgggcagga acgcactcgc gttcttgccg tcgagacggt cggcgttcag gttcgtcacc
     6001 acggttccgg agctgaccgt gaagggcgcg ccgctcgcga ccgtgaatga ggcggccggg
     6061 ccgccgcccg agttgttcgc cacgagcgac gcctgcgagc cgctgtcggt cgtggccttc
     6121 acgccgaatc cggtcaccga cgtgccgagc acgccgttgc cggctcccgt cgagcgtccc
     6181 cgaacggcgg aacccgtgcc ggttccgtcc agcgtgacca gcaaccccga gccggagctt
     6241 tgcgtcgtgg tgaggctcgt ctgcctggtg gcgctggtgt ccgcgcccgc cgtcagcggg
     6301 tcgccattgc tggccacggc gatattgctc cacacgatga cgagcagagc ggtgagggcc
     6361 aggatccccc ggctgctcca gttccgcatg gcaatctcct cccacgccag gcgggcaccg
     6421 agggactgat tctgtgccgt ctcccgaagc cagcgcaacg agccgatggc ctcccagcgc
     6481 ggcctgatcg tcagggctga cgaagcctga gccccggtct cccgccgggg ctcggtctcg
     6541 ttctcagtcc gcgtggtcga tcaaccattg gctcatgagc tgggttcggt ccacgattct
     6601 ggcccgagtc ggacgcttcg agggatgacc gggcgtagaa cccgcacgcg tgcaccgtga
     6661 agagcacggc ctcgaagatg gaccggtagt cctcatcgaa cgggcagttg acgaagacgt
     6721 tgtcctcgta tcgagcggtg gcggctggcg cgctacgagg acttgcgaga gaccgcggca
     6781 acggcctcct tgacctgctt ggccgtcagg cgcccatacc gcctggacgt cttgtacacg
     6841 accttcgcgg ccgaggggga gctcttcgcc gccgccgact tcgtcttcgt cgtcatctcg
     6901 acgtcagtcg acggcgctcc tgtcgcgatt cgccgtgatg gatcacagag gcagacctac
     6961 actgcggtcc atgacggagc ggatccctgt catcgagctc cgcgtggcac tcacggtcga
     7021 ggacctcgac gcggccgtcg ccttctaccg ggacgcgctc ggcctgccgg tcgagctgtc
     7081 gtgggacacc ccggacggcc gggggttcgt cctccaggcc ggccaggcga cgctcgagct
     7141 gatcgacgcc gctcaggccg acaccatcga ccgcgtggag gtcggccgcc gcacggccgg
     7201 tccggtgagg ctggcgctgg aggtcgggga ctcggaggcg accgcgggcc ggttggtcga
     7261 cgccggcgcc gagctgctcg gcggtcccgt ggagacaccg tggggcgacc gcaacgcccg
     7321 cgttcgcgcg cccgacggca tgcagctcac gctgttcacc cgaagcggct gaccggcgcg
     7381 ccggccgagc cgtcgagcgc ccggcggcca tgtcggtggg gcgtgccaga ctggccgcga
     7441 catggggggt cgttcgcgtg gggccccggc gcggcgcctc cgcgcggtcg gggccggcct
     7501 ggtcgtgttg atgctcgcga tcgcggggtg ccggtcggcg cgccaggccg aggccgcgca
     7561 cggtccctgt ccggcgggcg atccgctccg gggggtctac catcccgatc gcctccgggt
     7621 gctcgggacc tgcgtgacgt atcggggggt cgtccgcgac ttcaagcacg aatccgacgg
     7681 cgactaccac ctgtacgtca tccccgcgct cggcatggac cacttcctcg acccccgcag
     7741 cgaggggtac ggcgacctcg tcgtcgagct gctccccggg caggcgttgc ccctgccgca
     7801 acccggccag cgcgtcaccc tcgtcgggac gtgggcctac gacaccgacc acgactggaa
     7861 cgagctccat cccgtgtggg ccgagacgat ccggggccgc atccacaccg gccttccgcc
     7921 cgtgccgccc gagtacggcg gctcctcgaa cgcctgaatg cccgcccggg acggcccgcc
     7981 cgggacggcc cgcccggctc ggacacgcac ggacggcccg cccggctcgg acgcgtccga
     8041 ggaaccaggc gaggagatcg ctacagcggc cggcgctgcg gctcgacgcg cgggccgccc
     8101 gtgaacggga acgggtcggg gtgcgcgtac gtcaccgaga agtggtcggt gagccacccg
     8161 agcagtgcga acagcccgcc cacggcccac catcgccgga agtcgagcca gtgggcgccc
     8221 actgcgccga cggcgaagta gatgatcggg aacgccagga agtccttcag gtagtggcgg
     8281 atcgtgtggg tgagcggcgg gaggctgtcc ttctccttca ccaggctgat gatctccggg
     8341 aggaggaacc ccacgagggc cgccgggacc caggcccaga aggggatgaa gaacgcgagc
     8401 acgatcagtc cgggcccagt cacaaaccac atcaggatcc accacttgtt ccagtgctcc
     8461 ggggtctgaa gcgtgatccc caacgtggac agcgcgttcc tcaccttgcc catcgcggaa
     8521 cccctttcgg tcctcccgtc cccgcgggcg atcgcagcgt agtccggcgg ctccgctccc
     8581 gggacccggg cgcggggacc tcgctcggag tgacacgccg ccgcaaaggg aagggccccc
     8641 ggaagacttc ccgggggccc tctcgcggga gcccgcgttc ggtgtgttgt gcgcgccggc
     8701 gccggtccgg ctagagcgtc gccgagccgt tgcaggtctg gccggtcacg gtgttcgtcg
     8761 cctgcgcggt gaccgtgtcg ctgcccgccc ggtccgccgt catcgtccgg accttgaact
     8821 tgccgaggtc gttcgccgtc cgcgagccgc tgaagatctg cgtcccgttg tcggcgagct
     8881 gcacggtcca ctcctggccg ggcttggcgt cctccacgcg gaactgcacc tcgagcatgc
     8941 cgccgtcctc ggggctcacc ttgagcttcc agtcggcggc accggaacac gcgccccggg
     9001 tggtgacgcc gcccttggcc atcgccgccg gggccgcggc gaacatggcg ccgacgatca
     9061 tcccgacgat ccccaacctc acgatcctgt tcgctcgcat gtctcctcct tcgtccgtcc
     9121 gagcgatccg atggcgacag gttcggcgcg cgccggtcgg gcccacaagt ggacgggggt
     9181 cgcacgacca ccgggccgtt gttggcggga cctcgcggcg ccgagccttc atcgcgacgg
     9241 gccgcagggg gcggccggaa gcgaaaggag acggacgatg aggactcgca gggcgctggt
     9301 ggccctgtcg gtggccgcgg tggtggggct cgctcccacg gcggcgttcg ccaagcacgg
     9361 ggccgacgac cccaaggggc gccacgtgag ggaggaccgc gcgacggtca cccgcgtcat
     9421 ggacacccgc ggcaaggacg acccggccgg cgacgacaag ggccgccacg ccgagcccga
     9481 gcccggtgac gaccggggcg gcgccgcgcg ccacgctgag cccggggacg accacgggcg
     9541 tcacggcggc ggcgagccgg gcgacgacca cggcggtcgc tgaggaccgt gggctggttc
     9601 gcgcaggggc gcgtccggga ccgccgggcg cgcccttcgc tcgtcggggg ccggtccgag
     9661 ccgtcaggtt cgggggccga gtggtccgaa ctcgacgaga cctttgacct gggtttgccc
     9721 tcccattacc tgttcccctc taccgtagag acgcgggagg ggagggaacc catggcgcag
     9781 atcctgcacc gaccccgggc ccacgcccgg gtctccgacg acgaggaagg atcaagcggc
     9841 acgcgccgcg ggcgcggcgc cgctgccatg cgcgcgcagg cccgccggac gatcggtcgc
     9901 tccgtggccg tgttcgccgt ctccgcgctg gccgcggtgg tgatcgtggc cgtgatcgag
     9961 tcgttcgtgt tccgctcggc cggaaccgcc gaggccatcc gcgacgcgcg gcaggtcagc
    10021 gtgacggtcg gcaccgggat ggtcgaaccg gccctccgcg acgggatctt caccggagac
    10081 ccggcggcgc tgaactcgct cgaccgcgtc gtgaccgggc gcgtcctgag ccgttcgatc
    10141 gtccgcgtca acatctggga cgcctccggg cgcatcctct actcggacga acatcgcctg
    10201 atcggcgcca gctaccggct cggcgacgac gagagcgccg ccctccacgg cgatcgcccc
    10261 gaggccgacc tcagcgatct ctccgcgccc gagaaccggt tcgagcggcc gttcggctcg
    10321 ctcctcgagg tgtacatgcc ggtccacacg ccgaacggca ccccgctgct gttcgagtcc
    10381 taccaccgct acagcgcggt ggcggcgagc ggccggaagg tgtggctcgg cgtggtgccg
    10441 gcgctgctca tcggcctcgc cgtgctggcc ctggtgcagg tgccgctggc gtggcggatg
    10501 gcgcgaggcc tcgaacgcgg gcgcctggac cgcgagcggc tgctccgccg ggccgtcgag
    10561 gcgtccaccg ccgaacgccg ccggatcgcc ggggacctcc acgacggcgt ggtgcaggac
    10621 ttcgccggcc tgtcgatgtc gctggcggcg gcgggcgaac gggcggaggc cgacggcgca
    10681 ccggccgcgg cggccgcgct ccgcgagggg gccggcagga cccgccaggg catgcgccag
    10741 ctccgcaccc tcctggtcga gatctacccg ccgaacctcc acaccgccgg gctcgagccc
    10801 gcgctctccg acctgctggc tccgctcgcc gcgcgagcga tcctcacgca tctcgaggtg
    10861 gcgcccggcg tccacgccct ccaggccgac cgcgccatcg agggcctggt gttccgaacg
    10921 gcccaggagg ccctccggaa cgtggtggcc cacgcggagg ccgcatcggt cgaggtgcgg
    10981 ctcgccaccg ggcacggcat gctggagctg acggtgcggg acgacggccg cgggttctcc
    11041 accgccgatc tcgaccgaag cggcgaggaa ggccacatgg gcctcccgct cctggccggc
    11101 ctggccgagg aggcgggcgg gtcgctcgag gtggactccg agcccggcca cgggacctct
    11161 gttcgtctgg aggtgccggc cgcatgacga acacgaacgc gaactcgacc tcgaagtcga
    11221 acacccctga ggacgccacg gacgtgtccc gcagcatccg cgtgatggtc gtggacgacc
    11281 acgccgtggt tcgcgccggc ctggagcagc tcctcgccag tttccccgac gtcgaggtgg
    11341 tcggttcggc cggcggcggc gcggaggccg tggagctctc cgccgaacac gagcccgacg
    11401 tcgtgctcat ggatctctcg atgcccgagg tcgacggcat cgaggccacc cgccggatcg
    11461 ccgaacgcac cccggcgacg cgcgtggtga tcttcacgtc gttctccgag cgcgaccgga
    11521 tcctctcggc gatcgacgcc ggcgcgatcg gctacctgct gaaggacgcc gagcccgagg
    11581 agatccaccg cgggctccag gccgccgccc ggggcgagtc gccgctcgcc gcgaaggccg
    11641 cgcaggccgt ggtggcggcg ggcatcgagt cccgcccggt ccgggagcta accgaccggg
    11701 agcgggaagt cctcgtcctc gtggcgaccg gcctggcgaa caagcagatc gcccgccggc
    11761 tcggcatcac cgagaagacc gtgaaggccc acctcacgcg cctgttccag cggctcggcg
    11821 tcaccgatcg cacgcaggcg gccctgtggg ccgaacgcca cggcctcctc gccgcgagct
    11881 gaaccgctga gccccacgag cgaggcgtcg gttgactcgg gggtgaccgg ccgcgtctac
    11941 tctgcgtggg gcgccccacg tcgtctcgcc gtgcccgagt cgcgacactc gcgtcgaagc
    12001 cggtgaccgt gccgatgacc atctcggacg ggtcgggtgg ggacaccgtg cagggcggga
    12061 gcggggccga cgtgttcgtg ccggggacgt ccggcgctga agccgacagc tacgcgggcg
    12121 gcgcgggcgc cgaccacctc accgggtccg gcggcccgaa catcctcgcc ggggccgggg
    12181 gcgacgccgt gctcaagggc ctgggaggca acgacaccct gctgggcggc cccgggacga
    12241 cacgctgaac ggcggcaacg ggaccgacac ctgcaagcag ggcacgggca gcggttcgaa
    12301 gacgaattgc gaggcctgat caccctttca gccggtcgcc gggaacgcaa cgaacctccc
    12361 gttcgttgga ccatgtgcat gatgcgatcc accgatccga tccgggaggc attcgacatg
    12421 accgcgaagg tgcagaagag cgaagcggag tggcgggagc agctgtcgcc cgagcagttc
    12481 cacgttctcc gggagaaggg aaccgagcgg gcgttcaccg gcgcgtactg ggacgagcac
    12541 gccgacggcg tgtaccggtg cgcggcgtgc ggggccgagc tgttccggag cgacgagaag
    12601 ttcgactcgg gcacggggtg gccgagcttc tgggacccgg ccacatccga tgccgtcgag
    12661 ctgcaccacg acttcagcca cttcatgcac cgcgtcgagg tgacgtgcgc gacgtgcggc
    12721 tcccacctcg ggcacgtgtt cgacgacggt ccgcggccga ccggcaagcg ctactgcatc
    12781 aactcgtgct cgctggagct ggagacgacc acggccgcgg ggccctccaa ccccgcctga
    12841 cgggcgcccc gacctcgcgc gccggtccgc cggctcgggg gttgaccgtc cgaccggcgc
    12901 aacccatgct tcacgccttg gggcgcgccg gtcgtcggag ggggcgccac ggcagaggga
    12961 gggggcgtat gcgcccgagc ggcatgctca agctggtggt cgcggtcgca gtcctggggc
    13021 tcatcgtcgc gccgggggcc ggcgcgtcgg tggcgggcaa ggcg
//
LOCUS       DASPWO010000292         4442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_722402, whole genome shotgun sequence.
ACCESSION   DASPWO010000292 DASPWO010000000
VERSION     DASPWO010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722402"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VEO00_06740"
     CDS             complement(<1..1055)
                     /locus_tag="VEO00_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HYU57729.1"
                     /translation="MRPQPAPPPSHPQRQRAFMAGAAALTLPGLALRLSGGHPVEWLA
                     AVLFGVAIVGAAFLLAWAAEAVQLDVSQGLALALLALIAVLPEYAVDFVFTWKAGEDP
                     HEFAPLALANMTGANRLLIGVGWSMVVLLAAWRIRRAASAQGYKGPVDTQVELDRSHA
                     IEIAFLAVATIYSLTLPLKRTLTIADAAVLVTLFALYVARISRAPAEEPHLVGPAELI
                     GRLPTARRRTVVAGMFLFAAGAILACAEPFADSLVSTGEELGISTFLLVQWLAPLASE
                     APELLVAGLFAWRLNTNAALGALVSSKVNQWTLLVGTLPIVFSISAGVFHGLPIDTLQ
                     REELFLTAAQSAFAVTVL"
     gene            1200..1463
                     /locus_tag="VEO00_06745"
     CDS             1200..1463
                     /locus_tag="VEO00_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HYU57730.1"
                     /translation="MTLLDHYLPINQSWRQLARCRGVDPEVFYPVSDDDEAAEEAKSI
                     CVLCPVREACLEFALTTREKNGVWGGLTERERRRVLRRRRKTA"
     gene            1537..2703
                     /locus_tag="VEO00_06750"
     CDS             1537..2703
                     /locus_tag="VEO00_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU57731.1"
                     /translation="MNFDLPAELVDLQATVRKLAQERIAPRAREIDTTEEYPEDLFEL
                     FVETGLTGLCVPEEHGGSGAGILGLTVAIEEVAKYSNAAALMLLLTRLPTGPVMIAGS
                     DDLNRRYVTPLATGDARAAFGLSEPQAGSDVMGMRTKAVPDGDDWLLTGTKCWMSGVR
                     QADWYCVFAKTGPADSRAHDSVTAFIVERAWPGVEVGRTDRKMGVKGVDTGELILDSV
                     RVPAGNVVGEIGGFRLAMLGLNSMRPIVAARGIGLAEGALMYAVDYVQQREAFGQTIA
                     DFQGIQWKIAEMATEIEAARLLTYRSAWMADRGQFTKEWVPFLSMAKYYATELAVKVS
                     GEALQMLGAAGYMQDHPLELYYRDAKQLTIVEGTSQVQLGLIARGVLDRDLWWD"
     gene            2705..3769
                     /gene="trpD"
                     /locus_tag="VEO00_06755"
     CDS             2705..3769
                     /gene="trpD"
                     /locus_tag="VEO00_06755"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HYU57732.1"
                     /translation="MTGSPEPPLWPDVLGRLVRREDLPVELVERAMAAILEGQATDAQ
                     IAGFAVALRAKGETISELATLVRTMLSFSEPVELSGSAPVVDTCGTGGDRSHTINVST
                     LAALVVAGAGVRVAKHGNRAASSACGSADLLEALGVVIDLGPAGVARCIEDAGIGFCF
                     AQKFHPCMRFAGPTRKELGVATTFNFLGPLANPARVRRQVLGVSDRGMAERMAGALAE
                     LGSERALVFCGDDGLDELTTTTASTVHDLADGKVRTYSLDPAELGFAHAERGDLVGGD
                     AAHNAEITKRVLGGEPGPQRDVVVLNAAAALVAAGAADDIAAGVEGAQVSIDDGRASA
                     TLDAFVRASVAARDSEGEGT"
     gene            4202..>4442
                     /locus_tag="VEO00_06760"
     CDS             4202..>4442
                     /locus_tag="VEO00_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57733.1"
                     /translation="MAWPFRVIAWLVAVPIGLIVVVVPARKAGFLSGQRLLDVLVGTG
                     WNRYWRVLAIAPAWALVTAVLVQLMLLGMRKLGDRR"
BASE COUNT          638 a         1604 c         1612 g          588 t
ORIGIN      
        1 aggaccgtga ctgcgaaggc cgactgcgcg gcggtgagga acagctcctc gcgctggagg
       61 gtgtcgatcg gcagcccgtg gaacaccccg gccgagatcg agaacacgat cggcagcgtg
      121 ccgaccagca gcgtccactg gttgaccttc gaggacacga gcgcgccgag ggcggcgttg
      181 gtgttgaggc gccaggcgaa caggccggcc accagcaact cgggcgcctc ggacgccagc
      241 ggggccagcc actgcacgag caggaacgtg ctgatcccga gctcctcacc cgtcgacacg
      301 agcgagtcgg cgaagggctc cgcgcaggcg aggatcgcgc cggcggcgaa gaggaacatg
      361 cccgcgacca cggtgcgtcg acgggcggtc gggagccggc cgatcagctc tgccgggccc
      421 acgaggtggg gctcctcggc gggcgcccga ctgatgcgcg cgacgtacag cgcgaacagc
      481 gtcaccagca ccgcggcatc cgcgatcgtg agcgtgcgct tcagcggcag ggtgagcgag
      541 tagatcgtcg cgacggcaag gaaggcgatc tcgatcgcgt gcgaacggtc gagctcgacc
      601 tgggtgtcga ccgggccctt gtagccctgc gccgacgcgg cccggcggat gcgccacgcc
      661 gccagcagca ccaccatcga ccagcccacg ccgatgagga gacggttggc gccggtcatg
      721 ttggcgaggg cgagcggggc gaactcgtgg gggtcctcgc ccgccttcca ggtgaacacg
      781 aagtcgaccg cgtactcggg gagcaccgcg atgagggcga gcagggcgag ggcgaggccc
      841 tgcgagacgt cgagctggac cgcctcggcg gcccacgcga ggaggaacgc ggccccgacg
      901 atggcgaccc cgaagaggac cgccgccagc cactcgaccg gatggccgcc cgagagccgc
      961 agggccagtc ccgggagggt gagcgcggcc gcgccggcca tgaatgcccg ctgccgttgg
     1021 gggtgcgagg gcggcggggc aggctggggg cgcatcggcg tgatcctagg aaaccgcttg
     1081 atccggcccc ggtcccggcc ctacaccggg cgtttccccc tcaggcgcct gggaataggt
     1141 gacgaataac taggcgttga atcaattccc cccaccgttg cacgggtgag atcacgacca
     1201 tgaccctgct cgaccattac ctcccgatca accagtcctg gcgccagctg gcccggtgtc
     1261 gcggcgtcga ccccgaggtc ttctatccgg tctccgacga cgatgaagcc gccgaggagg
     1321 ccaagtcgat ctgtgtgctc tgccccgtgc gcgaggcctg cctcgagttc gccctcacca
     1381 cccgcgagaa gaacggcgtc tggggcggcc tcaccgaacg cgagcgtcgc cgcgtgctgc
     1441 gccgccggcg caagaccgcc taaccggcaa caagttcggg gccccgcagc gccctccgcc
     1501 cggggcgctg cggagaggcc ccgataggct caacgggtga atttcgacct cccggccgag
     1561 ctcgtcgacc tccaggccac cgtgcgcaag ctcgcccagg agcgcatcgc cccccgcgcc
     1621 cgcgagatcg acaccaccga ggagtacccc gaggacctct tcgagctctt cgtcgagacc
     1681 ggcctgaccg gcttgtgcgt ccccgaggag cacggcggct cgggggccgg catcctcggc
     1741 ctcaccgtcg ccatcgaaga ggtggcgaag tactcgaacg ccgccgccct gatgctcctg
     1801 ctcacccggc tgccgaccgg gccggtgatg atcgcaggaa gcgacgacct caaccgccgc
     1861 tacgtgacgc cgctcgccac cggtgacgcc cgggccgcgt tcgggctctc cgagccccaa
     1921 gccggcagcg acgtcatggg catgcggacc aaggccgtcc ccgacggcga cgactggctg
     1981 ctcaccggca cgaagtgctg gatgtcgggc gtgcgccagg ccgactggta ctgcgtgttc
     2041 gccaagaccg gcccggccga ctcgagggcc cacgacagcg tgaccgcgtt catcgtcgag
     2101 cgcgcgtggc ccggagtcga ggtcgggcgc accgaccgca agatgggcgt gaagggggtc
     2161 gacaccggcg agctgatcct cgacagcgtg cgcgtcccgg ccggcaacgt cgtcggcgag
     2221 atcggcggct tccggctcgc catgctcggc ctcaactcca tgcggcccat cgtcgcggcg
     2281 cgcggcatcg ggctggccga gggcgcgttg atgtacgcgg tcgactacgt acagcagcgc
     2341 gaggcattcg gccagacgat cgccgacttc cagggcatcc agtggaagat cgccgagatg
     2401 gccaccgaga tcgaggcggc gcgtctcctc acgtaccggt cggcgtggat ggccgaccgc
     2461 ggccagttca ccaaggagtg ggtgccgttc ctgtcgatgg ccaagtacta cgccaccgag
     2521 ctggcggtga aggtctcggg cgaggcgctc cagatgctgg gcgcggccgg ctacatgcag
     2581 gaccatccgc tcgagctcta ctaccgcgac gccaagcagc tcacgatcgt cgagggcacc
     2641 tcgcaggtgc agctcggcct catcgcccgc ggcgttctcg accgcgatct ctggtgggac
     2701 tgacgtgacc ggttcgcccg agccgcccct gtggccggac gtgctcggcc gcctggtgcg
     2761 ccgcgaggat cttccggtag agctggtcga acgggcgatg gccgcgatcc tcgagggcca
     2821 ggcgaccgac gcccagatcg ccgggttcgc ggtcgcgctg cgggccaagg gcgagacgat
     2881 ctccgagctg gccacgctcg tgcgcacgat gctgtcgttc tcggagccgg tcgagctgtc
     2941 cgggtcagcg ccggtcgtcg acacctgcgg caccgggggc gaccgctcgc acacgatcaa
     3001 cgtgtcgacg ctggccgcgc tcgtggtcgc gggcgcgggt gtccgtgtgg ccaagcacgg
     3061 caaccgggcc gcatcctccg cctgcggctc ggccgacctg ctcgaggcgc tcggggtcgt
     3121 gatcgacctc gggcccgccg gcgtggcccg ctgcatcgag gacgccggca tcggcttctg
     3181 cttcgcgcag aagttccacc cgtgcatgcg cttcgccgga ccgacgcgca aggagctcgg
     3241 ggtcgccacc accttcaact tcctcggtcc gctggccaac ccggcccggg tccggcgcca
     3301 ggtgctgggg gtgtcggatc gcggcatggc cgagcggatg gcgggggcgc tcgccgagct
     3361 gggcagcgag cgggcactcg tcttctgcgg cgacgacggc ctcgacgagc tcacgaccac
     3421 gactgcgtcg accgtccacg acctcgctga cgggaaggtg cgcacgtact cgctcgatcc
     3481 cgccgagctc gggttcgccc acgccgagcg cggcgatctc gtcggcggcg acgccgctca
     3541 caacgccgag atcaccaagc gggtcctcgg cggcgagccg ggcccgcaac gcgacgtggt
     3601 cgtgctgaac gcggccgcgg ccctggtcgc cgccggagcg gctgacgaca tcgccgccgg
     3661 cgtcgagggc gcgcaggtgt cgatcgacga cgggcgggcg tccgccacgc tggacgcctt
     3721 cgtgcgcgcg agcgtcgcgg cgcgcgattc cgagggcgaa gggacgtagg cccgtgcggg
     3781 tgggtctgtg cggaggcgcg tcccggaggg cggcgccggg taacccggcg ccgagcgccg
     3841 gagccatgtg atggcgaaag ctctgcaatg tccggcgtgt ggcagccggc acccactctc
     3901 cgaccttccg gacgcgccga cgttccgctg cacgggctgc gggcgggcgc tgaaggtccc
     3961 ggcccaagtg cgaccccgcc cgccgggccg gtcgggcgcc cctcggcccc gaccggcgga
     4021 ccggaccgcg accatgccgt cggcgccgcg tccccgcgcc acgcccgcgg gcgcggcggc
     4081 tgccggagca gccgccgccg gtgcagtggc ggcgccgccc gcgccgcctc ctgctccccc
     4141 aactccaccc gcggtgccgt cccggcgtcg tgggcccgag ccgggaccgg cgccacatcg
     4201 agtggcctgg cccttccggg tgatcgcctg gctggtggcg gtgccgatcg ggctgatcgt
     4261 cgtggtcgtt cccgcccgca aggccgggtt cctcagcggg cagcggctcc tcgacgtgct
     4321 cgtcggcacc ggctggaacc gctattggcg cgtcctcgcc attgcacccg cctgggcact
     4381 ggtcaccgcg gtgctcgtcc agctcatgct gctcgggatg cgcaagctcg gcgatcgccg
     4441 cc
//
LOCUS       DASPWO010000293         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1229298, whole genome shotgun sequence.
ACCESSION   DASPWO010000293 DASPWO010000000
VERSION     DASPWO010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229298"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VEO00_06765"
     CDS             complement(<1..1005)
                     /locus_tag="VEO00_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU57734.1"
                     /translation="MSLDPDTLGMILTALREFAAGRFPDQRLLDLDEKDEAPVEDLRA
                     MCGEDLGIHLLFLPEEYGGMGGGTFDVYRVSEQLARIDVGIATGVSVGSLGSDVISRG
                     GTAQQQKEWLTRIANEGLLFAYGATEPEAGSDLGALATTAVPVVEDGNVVGYRITGKK
                     QWISNGGIADAYSILANAPGGPTWFVIDAGVPGFEHGKPEDKQGLRTSNTAALFLEDV
                     FVNVDRRVGEAEGQGLLVAQTVFGYSRLMVAALGLGAGWAALERAIPYSVTRIQAGGP
                     LSEKQGYTHKLIVPHVVRLEAARSYIEESAERIDAGEGWLNTEGAIAKFMATEAGHAA
                     A"
     gene            complement(1176..1502)
                     /locus_tag="VEO00_06770"
     CDS             complement(1176..1502)
                     /locus_tag="VEO00_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57735.1"
                     /translation="MIKYVYEVDYPLGQKRNYLEWVRSIADTLQAPEELRRLASYDNV
                     FSATPNRVIEFTFDSLEDAGRYFERKEISRILQGELTAHGTNVGIKVLTRLADYSKDT
                     VTGDAP"
     gene            1847..>2863
                     /locus_tag="VEO00_06775"
     CDS             1847..>2863
                     /locus_tag="VEO00_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYU57736.1"
                     /translation="MTSPPSAAGSGGSGALRASSVVTLAGGPVPRRGRALNGPQVVSD
                     GVVGWRDGRIVYAGPASGWDGPVDERFEARTIVPGFVDCHTHLPFVGWRAEEFEARLA
                     GATYRELHAEGGIYRSARLMAEASDEEVLAFCRPLVAEMLDHGTTAFETKTGYGLSVE
                     RELRQARLAVALAAESPQTVVHTLLACHAVPRGMERQAWVDAVCAALVPRVAAEGLAG
                     AVDVYVEDIAFSVGDLEVVAAAADHHGLALRCHADQLGPSGAAEAAVRFGALSADHLN
                     HLSGAGIEALGAAPTAAVLLPSSSAFLGAPPPPARRLVGAGAAVAVATDFNPGTSPAL
                     SMPEA"
BASE COUNT          393 a         1045 c          977 g          448 t
ORIGIN      
        1 ggccgcggcg tggccggcct cggtagccat gaacttggcg atggcgccct cggtgttcag
       61 ccagccctcg ccggcgtcga tgcgctcggc cgactcctcg atgtacgagc gagccgcctc
      121 cagccggacc acgtgcggaa cgatcagctt gtgggtgtac ccctgcttct cggagagcgg
      181 cccgccggcc tggatgcgcg tcacggagta cgggatggcc cgctcgagcg cggcccaccc
      241 ggcccccagg cccagtgccg ccaccatcag ccggctgtag ccgaacacgg tctgggcaac
      301 gagcaggccc tgtccctccg cctcgccaac ccggcggtcg acgttcacga agacgtcctc
      361 gaggaacagg gccgcggtat tcgaggtccg gagcccctgc ttgtcctcgg gcttgccgtg
      421 ttcgaacccg gggacgccgg catcgatcac gaaccacgtc ggccctcccg gcgcgttggc
      481 caggatcgag taggcgtccg cgatgccacc gttcgatatc cattgcttct tgccggtgat
      541 gcggtacccg acgacgttcc cgtcctccac caccggcacg gccgttgtag cgagcgcgcc
      601 gagatcgctc ccggcctccg gctcggttgc cccgtaggcg aacaggagac cctcgttcgc
      661 gatccgcgtc agccactcct tctgctgctg ggccgtccca ccacgggaga tgacgtcgct
      721 ccccagggat ccgacggaca cgcccgtggc gatcccgacg tcgatccggg ccagctgctc
      781 gctcactcgg tagacgtcga atgtgccgcc gcccatgccc ccgtattcct cggggaggaa
      841 cagcaggtgg atcccgaggt cctcgccgca catcgcccgg aggtcctcga ccggggcttc
      901 gtccttctcg tcaaggtcca ggaggcgctg atccgggaag cgaccggcgg cgaactcccg
      961 gagcgccgtc aggatcatgc cgagcgtgtc cggatccaga ctcatcgacc gtctcccttg
     1021 gggtgcaaag ggctgtcccc tggcaccaac gtccagggaa gcatcccccc gtcgccatcg
     1081 ccacggtggg gttccccggc cctgatcctg cgggccgaac agccccccga tgtcctggtc
     1141 tcaggagtct caggacctac ccgttccggg aagcctcacg gggcgtcccc ggtcacggtg
     1201 tccttgctgt agtccgccag gcgggtcagg accttgatgc cgacgttggt gccgtgtgcc
     1261 gtgagctcgc cctggaggat ccggctgatc tcctttcgct cgaaatacct ccccgcgtcc
     1321 tccagcgagt caaacgtgaa ctcgatgacg cgattgggag tggccgagaa cacgttgtcg
     1381 tatgacgcca gccgtctcag ctcctccgga gcctgaagag tgtcggcgat cgatcggacc
     1441 cactcgaggt agttccgctt ctggccgagc gggtagtcga cctcgtacac gtacttgatc
     1501 accgaagaag ccccccctcg tcttcgtgcc ccacgcggct tcggcgcacc caccagcatg
     1561 gcacacggag ggttgggtag atgaacaccc caggcgacgt cgacggagcg ctcaccgcgg
     1621 gggcgatgcc ggaccccgag agaagaaagc caggggaggg gctgcccgcg aacccctccc
     1681 ctggcacatg cagcgttcga gcgccctctc gtgacgctgc ttcccaccgg cagagccggc
     1741 aggcccttcc gcgggacgca gcgggtctcg cggacgtcgg cctattgtgg cgacacggtc
     1801 atggggtacg tacaggggcc aaacgggtga tttttcatag ttcggaatga ctagtccccc
     1861 ctccgcggcc gggagcggcg gatccggcgc cctgcgggcg tcgtccgtgg tgactctcgc
     1921 cggcgggccg gtcccccgcc gtggccgtgc gctgaacggg ccccaggtcg tgtccgacgg
     1981 cgtggtcggg tggcgcgatg gccgcatcgt ctacgcggga ccggcgtccg gctgggacgg
     2041 cccggtggac gagcgcttcg aggcccggac gatcgtcccc ggcttcgtcg actgccacac
     2101 ccacctgccg ttcgtcggct ggcgggcgga ggagttcgaa gcccgcctgg ccggagcgac
     2161 ctaccgcgaa ctgcacgccg agggcggcat ctaccgcagc gcgcgcctga tggccgaggc
     2221 ctccgacgag gaggtgctgg cgttctgccg cccgctcgtg gccgagatgc tcgaccacgg
     2281 gaccacggcg ttcgagacga agaccggcta cggcctgtcg gtcgagcgag agctgcgcca
     2341 agcgcggctg gccgtggcgc tggctgccga gtcgccgcag accgtggtgc acacgctcct
     2401 cgcctgccac gcggtcccgc gcgggatgga acggcaggcc tgggtcgacg cggtgtgcgc
     2461 ggcgctcgtc ccccgcgtcg cggccgaggg cctggctggc gccgtggacg tctacgtcga
     2521 ggacatcgcg ttctcggtgg gtgacctgga ggtggttgcg gcggcagccg accaccacgg
     2581 actggcgctg cgatgccatg ccgatcagct cggcccgtcg ggcgcggccg aggccgccgt
     2641 ccggttcggg gcgctcagcg ccgatcacct caaccacctc tccggcgcgg gcatcgaggc
     2701 gctcggggcc gcaccgacgg cggccgtgct gctcccctcc tcctcggcct tcctcggcgc
     2761 gcccccgccg ccggcgaggc gcctggtcgg ggccggcgcc gccgtcgccg tcgccacgga
     2821 cttcaacccc gggacctcgc cggcgctctc gatgccggag gca
//
LOCUS       DASPWO010000294         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_44220, whole genome shotgun sequence.
ACCESSION   DASPWO010000294 DASPWO010000000
VERSION     DASPWO010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44220"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VEO00_06780"
     CDS             <1..948
                     /locus_tag="VEO00_06780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU57737.1"
                     /translation="LRALMRRQLDDVHERGEPLAALWASEGPIYGRFGYGLGSWSGSI
                     SVDRGHAAFQTATEASGRIAVLEKERAWPKMRQVYGRVWAGTAGFLGRDDTWWAYRFA
                     DPERHRDGAGALLYALYDGATGPEGYVAYRTKPAWPEGSPAGVLTIEELVASSPQAYA
                     ALWRLALDHDLIARVEAHRRPVDEPLLLMVADPRRLRFTLHDGLYVRLVDVAAALAGR
                     GYAREGSVVLEVRDAFCPWNEDRFAVEASADGASCEPTRAEPDLILDVSAVGAAYLGG
                     NTFAALVRAGAALEARPGAAAGADDLFRTERAPWCPHLF"
     gene            complement(1080..1580)
                     /locus_tag="VEO00_06785"
     CDS             complement(1080..1580)
                     /locus_tag="VEO00_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYU57738.1"
                     /translation="MKVAREIAFEDAIEASLLGPGCHARGLHTNYDPAVALDSAELFT
                     FIGVTQADAWEQVVERYGGDPDVAQRRFVGGLAGGLLPSPTAVVVLTGAVALHRVAFG
                     LALVVAFSVGLATALTAVGALALTARDATTRRLHGRLAAALPVLSAAGIIAVGTFLTA
                     RAALAF"
     gene            complement(1577..1981)
                     /locus_tag="VEO00_06790"
     CDS             complement(1577..1981)
                     /locus_tag="VEO00_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYU57739.1"
                     /translation="MIYVDTSVALAQLLAEDRHPSVTFWSSGPFVSSRLLQYEIWTRI
                     NAMGLAATHGEDVDQLLAGLAFLELAPAVLARALEPFPVPVRTLDALHLASIEFLRTQ
                     GQDVRLATYDGRLSGAAEALGVPLADLTADGS"
     gene            complement(1978..2268)
                     /locus_tag="VEO00_06795"
     CDS             complement(1978..2268)
                     /locus_tag="VEO00_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prevent-host-death protein"
                     /protein_id="HYU57740.1"
                     /translation="MRAVGLKVLKNKLSEYVRMAAAGETVLVTDRDRVVAELGPPREG
                     RGAMASSALLAQAVRDGLVTPPRIDRDGPPPRRPVATLSSVLKELEDDRADR"
     gene            complement(2405..2740)
                     /locus_tag="VEO00_06800"
     CDS             complement(2405..2740)
                     /locus_tag="VEO00_06800"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HYU57741.1"
                     /translation="MKTDDIELDEELLARAKRALGARTIRATVEEALRRAAEEAEGDQ
                     ARRAADQVRYLKRLHTRIDRQELASEGMWRQQAGFSTRAPRWADEPAEDLQGERPAPG
                     PRAVVLAPP"
     gene            2847..>3838
                     /locus_tag="VEO00_06805"
     CDS             2847..>3838
                     /locus_tag="VEO00_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU57742.1"
                     /translation="MTMETLPVTVDRSHLIVIGEKLYARSLELVRELANNAYDADARE
                     VSVQIGHDEVIVQDDGLGMDRDGLFRYFQVGVPDKRENPISSRLKRHRIGQFGIGKFA
                     TLAACRRFEVYTRRDAFSARVVFDRDDWERDEGDWRLPMEIDPQPGRKRDGTTVRMTK
                     LTKALDSNAVREYLVETVPLKAPEFSVYVNGLKLVPRSLAGRIPFLEGTPFGIVQGQA
                     VILPASRAAADSLGLEVKVRGVTVRRELFGMQGWGKLVTRVRGEVNADFLPLTADRST
                     FVIDSPEHRAFLEAMRKVMAEVEAALRQIALRKENRVARRAVVEALDRIEFALRR"
BASE COUNT          535 a         1413 c         1334 g          556 t
ORIGIN      
        1 ctgcgcgccc tgatgcgccg ccagctcgac gacgtgcatg agcgtggcga gccactggcc
       61 gccctctggg cctccgaggg cccgatctac gggcggttcg gctacggact gggctcctgg
      121 agcggctcga tctccgtcga ccgcggtcac gcggcgttcc agacggcgac cgaggcctcc
      181 gggcgcatcg ccgtcctgga gaaggaacga gcctggccga agatgcgcca ggtctacggc
      241 cgcgtctggg cgggaaccgc ggggttcctg gggcgcgacg acacctggtg ggcgtaccgg
      301 ttcgccgatc ccgagcgtca ccgcgacggc gccggcgcgc tcctctacgc gctgtacgac
      361 ggcgcgacgg ggccggaggg ctatgtcgcg taccggacca agcccgcgtg gccggagggc
      421 tcgccggccg gcgtgctcac gatcgaggag ctggtcgcgt cgtcaccgca ggcgtacgcc
      481 gcgttgtggc gcctggcgct cgaccacgac ctgatcgccc gcgtcgaggc ccaccgccgc
      541 ccggtcgatg agccgctgct gttgatggtc gcggacccgc gccggcttcg attcaccctg
      601 cacgacggcc tgtacgtccg gctcgtcgac gtggcggcgg cgctggccgg ccgcggctat
      661 gctcgcgagg ggtcggtggt actggaggtg cgcgacgcat tctgcccctg gaacgaggac
      721 cggttcgccg tggaggcgtc cgccgacggc gcgtcctgcg agccgacgcg agccgagccc
      781 gacctgatcc tggacgtctc agcggtcggc gccgcctacc tgggcgggaa cacgttcgcc
      841 gcgctggtgc gggcgggcgc cgcgctggaa gcccgccccg gcgccgcggc cggcgccgac
      901 gacctgttcc gcacggagcg cgccccctgg tgcccgcacc tgttctgagg cgctccctcc
      961 cgatccgcct aaaccatcac cggtgcggtg ccgaatggtt gggtgagaag catccccctt
     1021 ctcgacaggc cggatgggag gccgcggttc ccgaccgcgg cctcccgcct ttcgggcgct
     1081 cagaacgcca gggcggcgcg agcggtcagg aaggtgccga ccgcgattat cccggcggcg
     1141 gagagcaccg gcagcgccgc cgccaggcgg ccgtgaagcc ttcgggtggt ggcgtcgcgg
     1201 gcagtgagcg ccagggcgcc gacggccgtc agcgcggtgg ccaggcccac gctgaacgcc
     1261 acgaccaggg ccaggccgaa ggcgacccgg tgcagcgcaa cggccccggt cagcacgacc
     1321 accgcggtcg gtgacgggag caggccgccg gccaggcccc ccacgaaccg ccgctgtgcg
     1381 acgtcggggt cacccccgta tcgctcgacg acctgctccc acgcgtcggc ctgcgtgacc
     1441 ccgatgaacg tgaacagctc ggcgctgtcg agggccaccg ccgggtcgta gttggtgtgg
     1501 aggccacgcg cgtggcagcc tggccccagc agcgacgcct cgatcgcgtc ctcgaacgcg
     1561 atctccctgg cgaccttcat gacccgtcgg ccgtcaggtc ggccagcggc accccgagcg
     1621 cctctgccgc gccggatagc cggccgtcat aggtcgcgag ccggacgtcc tggccctggg
     1681 ttcgcaggaa ctcgatcgac gcgagatgaa gcgcgtcgag cgtccgcacc ggcaccggga
     1741 acggttcgag cgcccgcgcc aggaccgccg gcgccagctc caggaaggcc aggccggcca
     1801 gcagctggtc cacgtcctcg ccgtgggtcg cggccaggcc catcgcgttg atccgcgtcc
     1861 atatctcgta ctggagcagg cggctggaca caaagggtcc cgacgaccag aacgtgaccg
     1921 agggatgccg atcctccgcg agcagctggg ccagggcgac cgatgtgtcg acgtagatca
     1981 acgatcggcc cggtcgtcct cgagctcctt caggacggag ctcaaggtgg cgaccgggcg
     2041 tcgaggcggc ggcccgtccc ggtcgatccg cggcggcgtc accaacccgt cgcggaccgc
     2101 ctgggccagc agggcgctgg acgccatggc gccgcgcccc tccctcggcg gcccgagctc
     2161 ggccaccacc cggtcccggt cggtgaccag gacggtctca cctgctgccg ccatccggac
     2221 gtactcgctc agcttgttct tcagcacctt caacccgacc gctctcacgg tatccaaggt
     2281 agcttccaga agctacttcg tcaaccgacc ccgccgccgg atccgcgacc cgccggggag
     2341 ccagcgggtc cgtcctgtcg ggggaccgtg atgtccgaga cccgcgcgcc aggtggcagt
     2401 ctcctcatgg tggagccaga acgacggctc gggggcctgg cgccggcctt tcaccttgca
     2461 ggtcttccgc cggctcgtcc gcccatcgcg gcgctcttgt cgagaaccca gcctgctgcc
     2521 gccacatccc ctccgaggcg agctcctgac gatcgatcct cgtgtgcagc cgcttcaggt
     2581 accgcacctg gtccgctgct cgccgcgcct ggtccccctc cgcttcctcc gcggcccgcc
     2641 gcagcgcttc ctccacggtc gcccggattg tcctggcccc gagggcacgc ttggcccgcg
     2701 cgagcagttc ctcatccagc tcgatatcgt ccgtcttcat gtgcacatct tctatcggag
     2761 gctgaacaca ccccgggcgc attccgccct ggttcgtcct cccgcctggt tcccggccgc
     2821 aggctccacc tctatcattc ggcgcgatga ccatggaaac ccttcccgtc acggtcgacc
     2881 ggagccacct gatcgtgatc ggagagaagc tctacgcccg ctcgctggag ctggtccggg
     2941 agctggcgaa caacgcttac gacgccgacg ccagggaggt ctcggtccag atcggccacg
     3001 acgaggtcat cgtccaggac gacggcctcg gcatggaccg cgacggcctg ttccgatatt
     3061 tccaggtcgg cgtgcccgac aagcgggaga acccgatctc ctcgaggctg aagcgccacc
     3121 ggatcggaca gttcgggatc ggcaagttcg ccacgctggc cgcctgccgg cggttcgagg
     3181 tgtacacgcg acgcgacgcg ttctcggcca gggtcgtgtt cgaccgcgac gactgggagc
     3241 gggacgaggg ggactggcga ctccccatgg agatcgaccc ccagcccggc cgaaaacggg
     3301 acgggaccac cgtccgcatg acgaagctga cgaaggcgct cgactcgaac gccgtccgcg
     3361 agtacctcgt ggagaccgtt cccctgaagg cccccgagtt ctccgtctac gtgaacgggc
     3421 tgaagctggt cccccgcagc ctggccgggc gcatcccctt cctggaaggc accccgttcg
     3481 gcatcgtcca gggccaggcc gtgatcctgc cggcgtcgcg cgccgcggcc gactcgctcg
     3541 ggctggaggt gaaggtccgg ggcgtcacgg tccggcggga gctgttcggg atgcagggct
     3601 gggggaagct cgtgacccgg gtccggggcg aggtgaacgc ggacttcctt cccctcaccg
     3661 cggaccggag caccttcgtg atcgactcgc ccgagcaccg ggcgttcctt gaggccatgc
     3721 gcaaggtgat ggccgaggtc gaggccgcgc tccggcagat cgcgcttcgg aaggagaacc
     3781 gcgtggcccg ccgggcggtc gtagaggcgc tcgaccgcat cgagttcgcc ctccggcg
//
LOCUS       DASPWO010000295         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_385480, whole genome shotgun sequence.
ACCESSION   DASPWO010000295 DASPWO010000000
VERSION     DASPWO010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385480"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="VEO00_06810"
     CDS             <1..155
                     /locus_tag="VEO00_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU57743.1"
                     /translation="FVHVLMAGRIVKQGGPELAHELEEKGYEGIRAELGIAEVEAGAA
                     EPEGVR"
     gene            170..1420
                     /locus_tag="VEO00_06815"
     CDS             170..1420
                     /locus_tag="VEO00_06815"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HYU57744.1"
                     /translation="MTAQRAGLHRLDPEVVRKDFPILERTAHGKPLVYLDSANTSQKP
                     RQVIDAISDFYERHNANLYRGVYELSEEATAMFEGARAKLATFIGAPEPSSIVFTRGT
                     TESLNLVAHGFGRKVLREGDEVLVSEAEHHSNLVPWQLACNVTGASMRALPVADDYSI
                     DLSDLESTLTERTRVVAITGASNVLGTLPPLRRLADAAHAVGAVLVVDGAQLVPHTPV
                     DVVALDADVLTISGHKMLGPTASGGLYAKRDLLERMDPFLGGGEMILEVYLDRSTYKE
                     PPWKFEAGTMNIAEEIGLAAAIDYLQALGMEPVREHEVALTAYALERLASIGATVFGP
                     ADGETRGSAISFWLDDIHPHDLAQVLDEEGVCVRAGHHCAQPLMRRLGVPATTRVSFS
                     VYNTPGEVDVLIGALKRAREVFRG"
     gene            1417..1842
                     /locus_tag="VEO00_06820"
     CDS             1417..1842
                     /locus_tag="VEO00_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012575968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HYU57745.1"
                     /translation="MSLDDLYKEVILDHYKNPRNKRDLPGAEVTLSKNNPLCGDEITI
                     HAHLADGRVAEVTFEGQGCSISQASASMLTEAIAGKPMAEAEGLVRDFRGMMEGTVEP
                     DEDELGDLVALQGVARYPVRIKCAVLAWDILQDALADKT"
     gene            2389..>2984
                     /locus_tag="VEO00_06825"
     CDS             2389..>2984
                     /locus_tag="VEO00_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HYU57746.1"
                     /translation="MRVKPSTSWLLVLVPVSVLADFGLHNELLTFATAAGAIVPLAGT
                     IGRATDELALHAGPRIGGLLNATFGNVTELVIAIFLILDDEIEVVKAALTGSILGNLL
                     LVLGFSFLFGGLRHREQDFNAQSAGVHSSNLTLAVIGLLMPALFVISTGSRQFLEREV
                     VSGTVAAVLMTLYALALVFMLVTHEHLFRTPAPEEEPR"
BASE COUNT          433 a         1012 c         1087 g          452 t
ORIGIN      
        1 acttcgtgca cgtgctgatg gccgggcgca tcgtcaagca gggcggaccc gagctggcgc
       61 acgagctgga ggagaagggg tacgagggca tccgtgccga gctcgggatc gcggaggtcg
      121 aggccggggc cgccgagccc gagggcgtac gctagttcgg atggccagga tgacggccca
      181 gcgcgcgggg cttcaccgtc tcgaccccga ggtcgtccgc aaggacttcc cgatcctcga
      241 gcggacggcg cacggcaagc cgctggtgta cctggacagc gcgaacacga gtcagaagcc
      301 gcgccaggtg atcgacgcga tcagcgactt ctacgagcgg cacaacgcca acctctaccg
      361 cggggtgtac gagctgtccg aggaggccac cgccatgttc gagggcgccc gcgcgaagct
      421 cgcgacgttc atcggcgcgc ccgagccctc gagcatcgtg ttcacccgcg ggacgacgga
      481 gtcgctcaac ctcgtcgccc acggcttcgg ccggaaggtc ctgcgggagg gcgacgaggt
      541 gctggtctcc gaggcggagc accactcgaa cctcgtgccc tggcagctcg cctgcaacgt
      601 gacgggtgcc tcgatgcgcg cgctcccggt agcggacgac tactcgatcg acctctcgga
      661 cctggagtcg acgctcaccg agcggacgag agtggtcgcg atcaccggtg cctcgaacgt
      721 gctcggcacc ctgcccccgc tgcgccggct ggccgacgcc gcgcacgccg tcggcgcggt
      781 gcttgtggtc gacggcgcgc agttggtgcc ccacaccccg gtcgacgtgg tcgcgctcga
      841 cgccgacgtg ctgacgatct cgggacacaa gatgctcggc ccgaccgcgt ccggtggcct
      901 ctacgccaag cgcgacctgc tggagcggat ggacccgttc ctggggggcg gcgagatgat
      961 cctggaggtc tacctcgacc gctccacgta caaggagccg ccatggaagt tcgaagccgg
     1021 gacgatgaac atcgccgagg agatcgggct ggcggcggcg atcgactacc tgcaggcgct
     1081 ggggatggag ccggtccggg agcacgaggt cgccctgacc gcctacgccc tcgagcggct
     1141 ggcgtcgatc ggcgcgacgg tcttcggccc ggccgacggg gagacacggg ggagcgcgat
     1201 ctcgttctgg ctggacgaca tccacccgca cgacctggca caggtgctgg acgaggaggg
     1261 cgtctgcgtc cgcgccggcc atcactgcgc gcagccgctg atgcgccgcc tcggcgtgcc
     1321 ggccaccacc cgcgtctcgt tctcggtgta caacacgccc ggtgaggtgg acgtgctgat
     1381 cggcgcgctg aagcgggcac gggaggtctt ccggggatga gcctggacga cctctacaag
     1441 gaagtgatcc tcgaccacta caagaacccc cggaacaagc gcgatctgcc cggggccgag
     1501 gtgacgctct cgaagaacaa cccgctctgc ggcgacgaga tcaccatcca cgcgcacctc
     1561 gccgacggcc gcgtcgccga ggtcacgttc gagggacagg gctgctcgat ctcccaggcg
     1621 tcggcctcga tgctgaccga ggcgatcgcc ggcaagccga tggcggaggc ggagggcctg
     1681 gtccgggact tccgcgggat gatggagggc accgtcgagc ccgacgagga cgagctcggc
     1741 gacctcgtcg ccctgcaggg cgtggcccgc tacccggtgc gcatcaagtg cgcggtcctc
     1801 gcctgggaca tcctccagga cgccctcgcc gacaagactt gaccaaccag cgcccgcgcg
     1861 gtcccgcagg ccgcggtggc atcggtactg cggccgtcat catcggggcg atcggagtgg
     1921 cagttgggct gaccggcttc ctgtcgatct tcgcactccc gttcgcagcc ctggctctcg
     1981 tcttgggctt ctcgagcgtc acgacacctt gggcgcgggc gggctcccgt ggccatcgtg
     2041 ttcggaatcg tcgggctggt cctcggtacg gctatcttct gggagaggtt cttcttgccg
     2101 ccccgatagc ggagcgtttg cctgtcttcc gggcggtggg gatgcccgtc ggggagggcg
     2161 gccgcaggcc ggcgaagccg gcgagcgcag ggcggagtcg agcggagcct cggtcgcgtc
     2221 agcccgcctc gaagcgagtc acggctcccg ggagagccac gcggagcgag cgcagccgga
     2281 gcgatttcgg ccgcccgacc gtagacgggc gccccgcggc caccccgggc gtgtggggac
     2341 tcccggggcg ggagttccgc gggcatccgc cggggtagat tgcgcgccgt gcgcgtcaag
     2401 ccgtcgacgt cgtggctgct ggtgctggtg ccggtgtccg tgcttgcgga cttcgggctg
     2461 cacaacgagc tgctgacgtt cgccacggcg gcgggggcga tcgtcccgct ggccgggacg
     2521 atcgggcggg ccaccgacga gctggcgctg cacgccgggc cccggatcgg gggcctgctg
     2581 aacgcgacgt tcggcaacgt caccgagctg gtcatcgcga tcttcctgat cctggacgac
     2641 gagatcgagg tggtgaaggc ggcgctgacc ggctcgatcc tcgggaacct gctgctcgtg
     2701 ctcggcttct cgttcttgtt cggcgggctg cggcaccgcg agcaggactt caacgcgcag
     2761 tcggcgggcg tccactcgtc gaacctgacg ctcgcggtga tcggtctgtt gatgcccgca
     2821 ctcttcgtga tctccacggg cagccggcag ttcctggagc gcgaggtcgt cagcggcacg
     2881 gtggccgccg tgctgatgac gctctacgcg ctggcgctcg tcttcatgct cgtcacgcac
     2941 gagcacctgt tccgcacgcc ggcgccggag gaggaaccgc ggtg
//
LOCUS       DASPWO010000296         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1065545, whole genome shotgun sequence.
ACCESSION   DASPWO010000296 DASPWO010000000
VERSION     DASPWO010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065545"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VEO00_06830"
     CDS             complement(<1..209)
                     /locus_tag="VEO00_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYU57747.1"
                     /translation="MYQTDQYEGMIAETVAMRGHNGDFINAYLARPLGAGPFPGMVLI
                     HHAPGWDEWYRECTRRFAHHGYVSIS"
     gene            418..552
                     /locus_tag="VEO00_06835"
     CDS             418..552
                     /locus_tag="VEO00_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57748.1"
                     /translation="MEGEGEGRHRDSLVARAILRRRGLVVKEPISVSVAAGRWRWRST
                     "
     gene            complement(774..>2568)
                     /locus_tag="VEO00_06840"
     CDS             complement(774..>2568)
                     /locus_tag="VEO00_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HYU57749.1"
                     /translation="EIAVRQFQQQVLSTGLPQTTVWGYGSVNHPGTFQYPAQSIEATA
                     DKPVRVKWLNQLVDTSGNFLSHLLPVDQTLHWANPPGDCIVAPKRPDCRGQSQLRYDG
                     PVPIITHLHGAHVVPDSDGYPEAWYLPAAGNIDCIGAPGYPRTPATTDDFFCRGSNFD
                     QIPGAPVEDGAALFQYGNDQRATALWFHDHSVGMTRSNVYAGPAGFYFLRGGPADVSS
                     GVPGGLPGGVYEIPLVIQDRSFNADGSLFYPDSRVFFDGYAGPYIGDPLFASDAAPLH
                     NPEFFGNTMVVNGKTWPFLNVEPRKYRLRILNGSDSRFLILEFPANVKTKFTVIGTEG
                     GFLPAPVTLSQLLIGPAERFDVVVDFTGLKPGTVFNLLNVGPDSPFGGAFPASGQADA
                     TTTGQVMQLRVVALTAADTSAIPALPTITPISPTQTIRQVSLNEAESALICVDKNNVF
                     VPGVTPPVCGKRSAPLAPVMAQLGTIDPVTGLGIPKAFDDPITENIGLNTTEEWEISN
                     FTADAHPIHLHLVMFQVVSRQPFGGVARVPEVWETGFKDTVVAYPGEITRVKAVYDIP
                     GLFLWHCHILSHEDNEMMRPYCVGDPADCNP"
BASE COUNT          413 a          819 c          934 g          402 t
ORIGIN      
        1 gagatcgaca cgtagccgtg gtgagcgaag cggcgagtgc actcgcgata ccactcgtcc
       61 cagcccggcg cgtggtggat gagcaccatg ccgggaaagg ggccggcgcc gagcggtcgg
      121 gcaaggtacg cgttgatgaa atccccgttg tgccccctca tggccaccgt ctcggcgatc
      181 atgccttcgt actgatcggt ctggtacatg tccaccttcc ctccctgtga ggtgttcaag
      241 aaagcccgac ttcgccccgt cccattgctt gaccgcgctg cgccaccgcg ccgtgcagcc
      301 accccgccat gcaaggcctg gccgtacggg cgcacagggg cgatcgagca gcgtcacctc
      361 cgctcggccc gcctgtccgg gccgccctgc gcaagcgccg acggcggaat ggttcggttg
      421 gagggcgaag gtgagggccg gcatcgggat tcgctcgtcg cccgagcgat cctgcgccgc
      481 cgcgggctgg tggtcaagga gccgatcagc gtcagcgtgg cggcggggcg ctggcgatgg
      541 cggtcgacgt gacgagaagg gccgacaccg agcggctcgt cgccgagacg ctgagacatc
      601 gagggctggc tcgcccgcgg cgagcggccc tgagccgcgg cctcagccgg tcaggacacc
      661 cacgccggct tcggcgcggc gagtcggcgt ggctcggaaa agtcggaaaa gaaggagggc
      721 cgccatcagg cggccctcct tgcctccaag gtcgagactg gcgcctgacc agcttacggg
      781 ttgcagtcgg ccggatctcc gacgcagtac gggcgcatca tctcgttgtc ctcgtgcgag
      841 aggatgtggc agtgccacag gaagaggccc gggatgtcgt agacggcctt gacccgggtg
      901 atctcgccgg ggtaggcgac gacggtgtcc ttgaaacccg tctcccagac ctcgggcacg
      961 cgcgccacgc cgccgaacgg ctggcggctc accacctgga acatcaccag gtggagatgg
     1021 atgggatggg cgtcggccgt gaagttgctg atctcccact cctcggtcgt gttgagcccg
     1081 atgttctcgg tgatcgggtc gtcgaacgcc ttcgggatac cgagcccggt caccgggtcg
     1141 atcgtgccga gctgggccat gaccggggcg aggggcgcgg accgcttgcc gcagaccggg
     1201 ggagtgactc cgggcacgaa gacgttgttc ttgtccacgc agatcagcgc ggactcggcc
     1261 tcgttgaggc tcacctggcg gatggtctgc gtggggctga tcggggtgat ggtgggaagc
     1321 gccgggatcg cgctcgtgtc ggccgcggtg agcgccacca cccggagctg catgacctgg
     1381 ccggtcgtgg tggcatcagc ctggcccgac gccgggaagg cgccgccgaa cggcgagtca
     1441 ggacccacgt tgagcaggtt gaacacggtt cccggcttca agcccgtgaa gtcgacgacc
     1501 acgtcgaacc gctcggccgg gccgatcagg agctggctga gcgtcaccgg agcgggcagg
     1561 aagccgccct cggtgccgat cacggtgaac ttcgtcttca cgttcgcggg gaactccagg
     1621 atcaggaacc gcgagtcgga gccgttgagg atgcggaggc ggtacttccg cggctccacg
     1681 ttcaggaacg gccaggtctt cccgttcacc accatcgtgt tgccgaagaa ctcgggattg
     1741 tgcaggggcg cggcgtcgct cgcgaagagc gggtcgccga tgtacggccc cgcatacccg
     1801 tcgaagaaca cccggctgtc cgggtagaac agcgagccgt cggcgttgaa cgaccggtcc
     1861 tggatgacga gcgggatctc gtagacaccg ccggggagcc cgcccgggac gcccgaggac
     1921 acgtcagccg gtccgccacg gaggaagtag aagccggccg ggccggcgta cacgttcgag
     1981 cgggtcatcc cgacgctgtg gtcgtggaac cagagcgccg tggcccgctg gtcgttcccg
     2041 tactggaaga gcgccgctcc gtcctcgacc ggggcaccag ggatctggtc gaagttcgag
     2101 ccgcggcaga agaagtcgtc agtcgtggcc ggtgtcctcg ggtagccggg ggcgccgatg
     2161 cagtcgatgt tgcccgccgc gggcagatac caggcctcgg ggtagccatc gctgtcggga
     2221 acgacatggg cgccgtgcag gtgcgtgatg atgggaacgg gcccatcata gcgaagctga
     2281 ctctgccccc ggcagtcggg ccgcttgggg gcgacgatgc agtctccggg agggtttgcc
     2341 cagtgcagcg tctggtcaac gggcaggagg tgggacagga agttgccgct ggtatccacc
     2401 agctgattca gccacttgac ccggaccggc ttgtcggccg tggcctcgat cgactgcgcg
     2461 gggtactgga aggtccccgg atgattcacc gagccatagc cccacaccgt ggtctgcggg
     2521 agccccgtcg acaggacctg ctgctggaac tgtcgcacgg cgatctcg
//
LOCUS       DASPWO010000297         3755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_44731, whole genome shotgun sequence.
ACCESSION   DASPWO010000297 DASPWO010000000
VERSION     DASPWO010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44731"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VEO00_06845"
     CDS             complement(<1..293)
                     /locus_tag="VEO00_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYU57750.1"
                     /translation="MRRRLDVELLRRGLAGSRAEAALAIREGRVSVGGSPVVRAATLV
                     APEDPVAMAGPPRRFVSRGGEKLDAALDRFGIDVSGRRALDAGASTGGFTDCLL"
     gene            complement(301..753)
                     /locus_tag="VEO00_06850"
     CDS             complement(301..753)
                     /locus_tag="VEO00_06850"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HYU57751.1"
                     /translation="MLEAFRQYVEAGLGALPKDRAMELARQLLAQGQGLLEQGQSLVG
                     QAPGGEQVQRLARDLMSWSRASGERLRDMVQEQVTKQVKAMGLATRDDVDDLRRRIRE
                     LEARGATKKRAAKRAPAKKRTAKRRTAKKRTVKAAARTGTSGGSGSGA"
     gene            940..1293
                     /locus_tag="VEO00_06855"
     CDS             940..1293
                     /locus_tag="VEO00_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HYU57752.1"
                     /translation="MATKHEVEAKLRVLIERLDGAGDGVAETLADALPEAKVVGLHVT
                     DLDAHWWTEMSDGRFSALAPGQPDGADIRIAMTSDDLVDLVDGRANLFSKVVAGHVRV
                     DASLSDLLRLRRLAG"
     gene            1331..2494
                     /locus_tag="VEO00_06860"
     CDS             1331..2494
                     /locus_tag="VEO00_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYU57753.1"
                     /translation="MKRGHRFMAASIGAVAAVAAAGRAASLRGRRDDGEEELLGSVRG
                     EPLTLRGPRASRIYAERLAGTRGTVVLTHGWCLTESVWHYQKRALADAGFTVVAWDLP
                     GHGASTPVARGHLTLDLAVDALARVVEHVADPAGMILVGHSLGGVLTLRYLTSGGCEP
                     PDSVRGVVFASTPMMHFARSVAGSWPGAGLEARALGAAMEFIVANPALDRIFQAGVGG
                     NAYPLSYRVVRVGFGRRPVPAQVRFVRDVIATVPPHVRVDTYKAMTGYDLSDDVRSVK
                     LPALVVIGERDRLINPTESREMAARLPRARVVAFPRAGHASFLEEHERFDRELIAFCE
                     RRLAAPPKLAAPAKGAPSGGSGGNEEGRLRRLRRAPRPPGGRRPSRAPRSTRA"
     gene            2499..3335
                     /locus_tag="VEO00_06865"
     CDS             2499..3335
                     /locus_tag="VEO00_06865"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYU57754.1"
                     /translation="MLPLKDRNPTERTAYLTLAIIGLNVAVFLILQKPFDTSNEVIRT
                     SQGTIEAPAGTTFIFCHALIPWEFANGKPLAQGGADAEEALRASFGSDGGLFLQLLDA
                     KCPDKSVWFSFLSSMFMHGGWLHIAGNMLFLWVFGNNVEDKIGPVGYLVFYLAAGVIA
                     AFTHIFSGAQSAVPTVGASGAIAGVLGAYLVMFPRRRVVTLLMFFFITVVEIPAYVLL
                     GFWFVLQFFQGIASIGGDVTSVAVWAHVGGFAAGVLAALALFPKERGDPNRPAYPLLP
                     SP"
     gene            complement(3311..>3755)
                     /locus_tag="VEO00_06870"
     CDS             complement(3311..>3755)
                     /locus_tag="VEO00_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HYU57755.1"
                     /translation="DVVVVGWDGGLTYAALRRAALLVQRGARLVATNDDATLPAPDGL
                     WPGAGAILAAVVTASGGVPLVVGKPHRTLLEVVAAAAGDGPVLAIGDRLETDVAGAAA
                     MGWDSALVLTGAARREDVAGSGQRPTYVVDSIADLVAVQGEGRRG"
BASE COUNT          464 a         1445 c         1310 g          536 t
ORIGIN      
        1 agcaggcagt cggtgaaccc tccggtggac gccccggcat cgagggcccg gcggccggac
       61 acgtcgatgc cgaaccgatc gagcgccgcg tcgagcttct ccccgccacg cgagacgaag
      121 cgccgcggcg ggccggccat cgccaccggg tcctcgggcg ccacgagtgt tgcggcgcgc
      181 accaccggcg atccgcccac ggacacccgg ccctcgcgga tggcaagcgc ggcctcggcc
      241 cggctgccgg cgaggccgcg acggagcagc tcgacatcga gcctgcgcct cacccgcgga
      301 tcaggccccg cttccggatc cgccggaggt gcccgtcctg gccgccgcct tcacggtgcg
      361 tttctttgcc gtgcgtctct ttgccgtgcg cttcttcgcc ggcgcgcgct tcgcggcgcg
      421 cttcttcgtc gccccacggg cctcgagctc gcggatccgg cggcgcaggt cgtccacgtc
      481 gtcccgcgtg gccaggccca tcgccttgac ctgcttcgtg acctgctcct ggaccatgtc
      541 gcggaggcgc tccccgctcg cgcgcgacca gctcatcagg tcccgcgcca acctctgcac
      601 ctgctcgccg ccgggcgcct gcccgaccag cgactgtccc tgctccagca ggccctggcc
      661 ctgcgcgagc agctggcgcg cgagctccat cgcgcggtcc ttcggcagcg ccccgaggcc
      721 ggcctcgacg tactgccgga acgcctcgag catcaccctc ctccttcgat cgtccgttgt
      781 gatcccgcca ccgcggggtc ccgccgaaca ctgcccccac ccccgcacga aggatagacc
      841 gcggggacga cgcccggacc caatcgtccg agccggtata ctccaggccc ccgcgatgcc
      901 ttccccaccc cccgtcttcc ctgagggcgg atggacgccg tggccacgaa acacgaggtc
      961 gaggcgaagc tccgggtgct gatcgagcgc ctggacggcg ccggcgacgg cgtcgcagag
     1021 acgctcgccg acgccctccc ggaggcgaag gtggtgggac tccacgtcac cgatctggac
     1081 gcgcactggt ggaccgagat gtcggacggc cgattcagcg cgctcgcgcc cggccagccc
     1141 gacggcgccg acatccggat cgcgatgacg agcgacgacc tggtcgacct ggtggacggc
     1201 cgggcgaacc tgttctcgaa ggtcgtcgcc gggcacgtcc gggtggacgc cagcctgtcg
     1261 gacctgctgc ggctgcggag gctcgcgggg tgaggatcac cagcccgcgg ccccggcgga
     1321 ggacggttcg atgaagcgcg gccaccggtt catggcggcc tcgatcgggg ccgtcgcggc
     1381 cgtcgccgcg gccggacgcg ccgcgtcgct ccgcggccgg cgcgatgacg gcgaggagga
     1441 gctgctcggc tcggtccgcg gcgagccgct caccctgcgc ggaccgcggg cctcgcggat
     1501 ctacgcggag cgcctcgccg ggacccgggg gaccgtcgtg ctcacccacg gctggtgcct
     1561 gaccgaatcg gtctggcact accagaagcg ggccctcgcc gatgccggct tcacagtggt
     1621 ggcgtgggac ctccccggcc acggcgcgtc cacaccggtg gcccgcggac acctgaccct
     1681 cgacctcgcg gtggacgccc tggcgcgcgt cgtggagcac gtcgccgacc cggccgggat
     1741 gatcctggtc ggccacagcc tgggcggcgt gctgaccctg cgctacctga cctccggcgg
     1801 ctgcgagccg ccggactcgg tccgcggggt ggtcttcgcc tcgacgccga tgatgcactt
     1861 cgcccggtcc gtggccgggt cgtggccggg ggccggcctg gaggcccggg ccctcggggc
     1921 ggcgatggag ttcatcgtgg cgaacccggc gctcgaccgg atcttccagg cgggggtggg
     1981 cgggaacgcc tatccgctct cctaccgcgt cgtgcgcgtc gggttcggca ggcggccggt
     2041 gccggcgcag gtgcgattcg tgcgcgacgt catcgccacg gtcccgcccc acgtccgggt
     2101 ggacacctac aaggcgatga ccggctacga cctctcggac gacgtccggt cggtcaagct
     2161 ccccgccctg gtcgtgatcg gcgagcgcga ccgcctgatc aaccccaccg agagccgcga
     2221 gatggcggcc cgcctgccgc gcgcccgcgt tgtggccttc ccccgggccg gccacgcctc
     2281 gttcctcgag gagcacgagc gattcgaccg cgagctgatc gcgttctgcg agcgccgcct
     2341 ggcggcgccg ccgaagctcg ccgcgccggc caagggggcc ccgtccggcg ggagcggcgg
     2401 gaacgaggag gggcgcctgc ggcgcctgcg ccgggccccg cgaccacccg gtggccgacg
     2461 gccgtcccgc gcgccccgat cgacgcgggc gtagcccggt gctccccctc aaggaccgca
     2521 atccgaccga gcggacggcc tacctgaccc tcgcgatcat cggcttgaac gttgccgtgt
     2581 tcctgatcct gcagaagccg ttcgacacct cgaacgaggt gatccgcacc tcgcagggaa
     2641 ccatcgaggc gccggccggg accacgttca tcttctgcca cgccctgatc ccctgggaat
     2701 tcgccaacgg gaagcccctg gcgcagggcg gggcggacgc cgaggaagcc ctgcgcgcgt
     2761 cgttcggatc ggatggcggg ctattcctcc agctcctcga cgcgaagtgc cccgacaaga
     2821 gcgtgtggtt ctcgttcctg tcgtcgatgt tcatgcacgg cgggtggctg cacatcgccg
     2881 ggaacatgct gttcctgtgg gtgttcggga acaacgtcga ggacaagatc ggcccggtcg
     2941 ggtacctggt cttctacctg gccgccggcg tgatcgccgc gttcacgcac atcttctccg
     3001 gagcgcagtc ggccgtcccg accgtgggcg cgtccggcgc gatcgccggc gtcctcggcg
     3061 cctacctggt gatgttcccg cgccggcggg tggtgacgtt actgatgttc ttcttcatca
     3121 ccgtcgtgga gatccccgcg tacgtgctgc tggggttctg gttcgtgctg cagttcttcc
     3181 aggggatcgc ctcgatcggc ggcgacgtca ccagcgtcgc ggtgtgggca cacgtcggtg
     3241 gcttcgcggc cggggtgctc gccgcgctgg cgctcttccc caaggagcgc ggcgacccga
     3301 accggccggc ctatcccctc ctgccttcgc cctgaacagc caccaggtcg gcgatcgagt
     3361 cgacgacgta ggttggccgc tggcccgacc ccgcgacgtc ttcccggcgg gcggcgccgg
     3421 tcaggaccag cgcggagtcc cagcccatcg ccgcggcgcc ggcgacgtcg gtctccaagc
     3481 ggtcgccgat cgcgagcacc ggcccgtcgc cggccgccgc ggccaccacc tcgagcagcg
     3541 tgcgatgcgg cttccccacc accagcggaa ccccaccgct ggcggtgact accgcagcga
     3601 ggatcgcccc ggcccccggc cacagcccgt ccggggccgg aagcgtggcg tcgtcgttcg
     3661 tcgccaccag ccgcgccccg cgctggacga ggagcgccgc gcgcctcagc gcggcgtagg
     3721 tcagcccgcc gtcccacccg acgacgacca cgtcg
//
LOCUS       DASPWO010000298         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1145640, whole genome shotgun sequence.
ACCESSION   DASPWO010000298 DASPWO010000000
VERSION     DASPWO010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145640"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VEO00_06875"
     CDS             <1..411
                     /locus_tag="VEO00_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57756.1"
                     /translation="AVLVASLVDVPLRGGYGQRIFQPLATTSVPPAYRLVAGEIQIDL
                     RQLAIQGQTVNVTATVGAGHIAVFAPAGVRLVVHGRAGAGQIDLLGVTYAGIRVSVDR
                     TFGPTSPLGSIVLDLRTGLGAIDVYRAPGAGVAG"
     gene            408..680
                     /locus_tag="VEO00_06880"
     CDS             408..680
                     /locus_tag="VEO00_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57757.1"
                     /translation="MRRHGFDALSFAFGLIFAVMGAAFVIGGADVFDHRFDRLWPFAL
                     IAVGLATIAAGAGWGRTPRTRPDVADAPPETEPETDEEEEEPVSSS"
     gene            complement(667..954)
                     /locus_tag="VEO00_06885"
     CDS             complement(667..954)
                     /locus_tag="VEO00_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57758.1"
                     /translation="MDDREKEIAELLHDAGETHHVVFAITQGADDDWASWYSDWLTNL
                     SKLPELLGTRPVRSELTYMLVRLDKEYVERAPSERWEDYYARALIEHFTTS"
     gene            1071..1664
                     /locus_tag="VEO00_06890"
     CDS             1071..1664
                     /locus_tag="VEO00_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYU57759.1"
                     /translation="MLAIVSSNVVIIAVVAGIPAVVAVLVVLWSLPRRPSAVIGVASV
                     LAIALVAVSVWAVFRSSTETAAAGPSIPAGPSIPAPSLPSTSSPPAPGGGCAPSGTNL
                     QLTAKGIAFDKSCLAAPAQTAFTLAFDNQDAGTLHNIHILAGDPASNPGAQSLFTGEF
                     VTGVATTDYQIGALPPGTYYFQCDVHPPQMHGTFIVK"
     gene            1764..>2339
                     /locus_tag="VEO00_06895"
     CDS             1764..>2339
                     /locus_tag="VEO00_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57760.1"
                     /translation="MQHLRKVLAAAFAVAAVMSIPGSTAGAAPRSRVTEFIVPSAGSR
                     PYQIVAGPDGNLWFTESGAGTVGKITPDGHIIEYKIRKDSAPYGITVGPDGNIWFTEK
                     DPAPDGSTRVGKLTTDGELTEYLVSAFSQPWDIVSGPDGALWFTEEEADLIGRITTDG
                     GLTEYATGTCCFPTQITVGFDGNLWFTEEQGP"
BASE COUNT          346 a          791 c          811 g          391 t
ORIGIN      
        1 gcggtcctgg tggccagcct ggtagacgtg cccctgcggg gaggatacgg gcagcggatc
       61 ttccagccgc ttgcgaccac cagcgtcccc cccgcctacc ggctggttgc gggtgagatc
      121 cagatcgacc ttcgccagct ggcgatccag ggccagacgg tcaacgtcac ggcaaccgtg
      181 ggcgcgggcc acatcgcggt cttcgcgccc gccggtgtcc ggctcgtggt gcacggacgg
      241 gccggagccg gacagatcga cctcctcggc gtgacctacg cggggatccg ggtctccgtg
      301 gatcgaacgt tcggaccgac ctccccgttg gggagcatcg tgctcgacct ccgaacgggg
      361 ctgggagcga tcgacgtgta ccgggcaccg ggagcggggg tggcgggatg aggcggcacg
      421 ggttcgatgc gctgtcgttc gcgttcgggc tgatcttcgc tgtaatgggg gccgcgttcg
      481 tgatcggcgg cgcggacgtg ttcgaccatc ggttcgaccg gttgtggccg ttcgcgttga
      541 tcgcggtcgg cctggccacc atcgccgccg gggcggggtg gggtcgaacg cctcgaacgc
      601 ggcccgacgt ggccgatgcc ccaccggaga ccgaacccga gacggacgaa gaggaagagg
      661 agcccgtcag ctcgtcgtga agtgctcgat gagcgcgcgg gcgtagtagt cctcccatcg
      721 ctcggagggg gcgcgctcga cgtactcctt gtcgaggcgc acgagcatgt aggtcagctc
      781 gctccggacg ggcctggtcc ccagcagctc cggcagcttc gacaggttcg tcagccagtc
      841 cgagtaccac gacgcccagt cgtcgtcggc tccctgggtg atggcgaaca cgacgtggtg
      901 ggtctctccg gcgtcgtgca acagctcggc gatctccttc tcccggtcgt ccatggccct
      961 ccctatcgat gatcgcctgc agcgcgccat ccgattggcg tacgcctgct gaccgggtag
     1021 gggccccggc cggccctgtt ccgccggatt cttcactatg ctgagtggcc gtgctggcga
     1081 tcgtttcgtc gaacgtggtg atcatcgccg tggtggccgg gatcccggcg gtcgtggcgg
     1141 tcctggtcgt gttgtggtcg ctgccccggc ggccgtccgc cgtcatcggc gtggcgtcgg
     1201 tgctggccat cgcgctggtc gcagtgtcgg tgtgggccgt gttccggtcc tcgaccgaga
     1261 ctgccgcggc ggggccctcg atccccgcag gaccgtcgat ccccgcacct tctctgcctt
     1321 cgacgagctc ccctcccgcc ccgggcggcg ggtgcgcgcc gagcgggacg aaccttcagc
     1381 tcacggccaa ggggatcgcc ttcgacaagt cctgccttgc cgcaccggcc cagacggcct
     1441 tcaccctggc cttcgacaac caggacgcgg ggacgctgca caacatccac atcctcgccg
     1501 gcgacccggc gagcaacccg ggggcgcaga gcctgttcac cggcgagttc gtcaccgggg
     1561 tggccaccac ggattaccag atcggggctc tcccgcccgg cacgtactac ttccagtgcg
     1621 acgtgcaccc gccccagatg cacgggacct tcatcgtcaa gtgatgcaaa cggtgatccg
     1681 gaggcacacg cgctccgact gaccctgtgg aatgggcgat cctagggtat ggtcgtcgcc
     1741 ggaccgtctg gaggggagcg ctcatgcaac atcttcggaa agtgcttgcc gcggcgttcg
     1801 ccgtcgccgc ggtgatgtcg atccccggca gcacggcggg agccgctcca aggagccggg
     1861 tgaccgagtt catcgttccc tcggcgggga gccgcccata ccagatcgtg gccggccccg
     1921 acgggaacct gtggttcacc gagtccgggg cgggcaccgt cgggaagatc acacccgatg
     1981 ggcacatcat cgaatacaag atccggaagg acagcgcgcc gtacgggatc acggtgggtc
     2041 ccgacggcaa catctggttc accgaaaagg atccggcgcc cgatgggagc acccgggtcg
     2101 gcaagctgac caccgacggc gagctcaccg agtacctcgt ttccgccttc tcgcagccgt
     2161 gggacatcgt ctccggtccc gacggcgcct tgtggttcac ggaggaagag gccgacctca
     2221 tcgggaggat caccaccgat ggggggctca ccgagtacgc cacgggaacg tgctgtttcc
     2281 caacccagat caccgttggg ttcgacggga acctgtggtt caccgaggaa cagggcccg
//
LOCUS       DASPWO010000299         8623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1230163, whole genome shotgun sequence.
ACCESSION   DASPWO010000299 DASPWO010000000
VERSION     DASPWO010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230163"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VEO00_06900"
     CDS             complement(<1..487)
                     /locus_tag="VEO00_06900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HYU57761.1"
                     /translation="MPAASVVVLCRNDHGTLGRVLEGLLGQRTEEPFEVVLVDSSDDG
                     GGAALATLGTWAFELEVVTSPVGLTTGAARNAGMARARGRYLLFLAADCIPDPDWVGR
                     RVGWHRAGHPVVAGAVTCATPRTLAARVQWLSRFSGALPSGGPRRRTLPQFAWSYDRE
                     IL"
     gene            complement(500..1657)
                     /locus_tag="VEO00_06905"
     CDS             complement(500..1657)
                     /locus_tag="VEO00_06905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU57762.1"
                     /translation="MNTPWDIVCFAGVDWDSHRQRPQWLMQGFADRGASVLYVDNLGT
                     RLPRISDARRVAHRVSRWARSSWTPTTERPGGAGRILRDAPVVLPLQHLALVRAFGRW
                     TLLHRLRRRVGGGRPLVVWTYLPMPVIRDAAAGLGADLLVYDWSDDASAHVLTASPAV
                     RRRIAGWETAMAKAADLVFVASAELLRRRGGGNPETHLIPHGAPDSPRSTTRVPHAVD
                     RLPHPRIGFVGSITEWTDVGLVEELAASRPDWSFVLVGPVRIRVNGLRARDNVLLTGE
                     RPHEEIPALLRAFDAGIIPYRVTPATTAASPVKLREYLAHGLPVVSVDVPEVRPFAPP
                     VRLATGADAFAEALAEALAEGRRPPGRGAPTWDERVDEMAAHVARALERAR"
     gene            complement(1728..2444)
                     /locus_tag="VEO00_06910"
     CDS             complement(1728..2444)
                     /locus_tag="VEO00_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYU57763.1"
                     /translation="MPTSLAPAARRSVVTTMAGTPLLSDLARPAVEGLAGSGRLRSYR
                     RGTYLFHQGDPADEVFFLVAGRIEIGTLSSTGHRQLITALDHPEFFGEFGVLGHMPRT
                     ATAIALEESDVWVTGSADFLGFLEREPRATLALLKALARQIAAMEAHVEDLLFLDLKG
                     RVAKRLLGMVVPSLEEIPEDGAIVPSEITHADLAALSGGSRENVTRILSELARRGLVE
                     RSGRRYVLKDVAGLRRLAGL"
     gene            complement(2556..2753)
                     /locus_tag="VEO00_06915"
     CDS             complement(2556..2753)
                     /locus_tag="VEO00_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57764.1"
                     /translation="MAEKKVTPKKPSGGTAKSGASKAAKQVKAVRATRATRATRATRA
                     VRATRAVRASRAVRASRASRS"
     gene            complement(2874..3179)
                     /locus_tag="VEO00_06920"
     CDS             complement(2874..3179)
                     /locus_tag="VEO00_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57765.1"
                     /translation="MDCWHCRRTAVGTCRFCGRGVCEDHALTHPYVLELYRSAGVPRA
                     LVVEDALYCGACTPRPDPIDLPELDMDDDSGGLGGARRPGTDRPDRVRRGGPPTGEE"
     gene            complement(3209..5371)
                     /locus_tag="VEO00_06925"
     CDS             complement(3209..5371)
                     /locus_tag="VEO00_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYU57766.1"
                     /translation="MSGPPGGLWEGLADAVAGQPTDLERDEGTWVTLAERADPGAFRP
                     ALAPDIEVKSFPTRWGEGYAMAANPREMLHYRLEAGEVELLPLMDGTRTVKEIVVERF
                     RESGEMELDGVADLVRQLHEGNFLQSHFTDVEGMIDKALNPETTVGRKTREFVTDLRL
                     EWGNADRFVRWLYRRGLRVLFTRPAMALSVLVALAGLVAFFANVRSSRFDLAGQSLAI
                     GFLLLLLLQLFHIFLHELGHGSVLVHYGRKVKAAGFFIFFGAPAFYIESSDGLMLERR
                     KRIIQSAAGPFAGLLVAGATSIYVYLVPAGAISALLYKFTVLAYLAVFMNSLPMLELD
                     GYWILADALQIPDLRPRSIAFVRHDLARKLWRRERFTSREAGLALYGVVGITFTIFSF
                     YTAYFFWEAVFGGLVGRLWAGGTTTRLILIALGLFVGGPLIRGLVGLVRSLWLRVKAV
                     ADRIRFHLQTGWRVEAATLIDALPLFDDVPEDVLSDLAGRVRLHTVARDREVVRQGDR
                     ADAFYVVRHGTLQVEEENPETGARRVLRKLGRGESFGELGLLRGTPRAVTVRALEPSQ
                     IFQIDESTFDRLLADMIKVPSFAPVLQETIELRELRAFAHLEPSEASEVLAQGEWTDM
                     PPGEVVMEQGETGDAFYAIGSGQVEVRIDGERKATLGRGDYFGEVALLMDVPRTATVV
                     TLTPVRAFRLGREGFDRLIARSFRRGTLDPHMPSERPS"
     gene            complement(5368..6060)
                     /locus_tag="VEO00_06930"
     CDS             complement(5368..6060)
                     /locus_tag="VEO00_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57767.1"
                     /translation="MHRTVAWGALFVLAGSVVPGFAARAAVAQAGGVTISGDAVGETV
                     DGGLLTVTAEAIDPAGWEDVREIAVELRLRGRSLDRLVVDPAQAVVAIAGEGAAQIAG
                     TEGVIEGAFFVVDVSRVVIAAGGQRVRVTATIRFAADPSPGSRLVLTAAGPPGVPVRT
                     VLLRRIPEPRGGVSVGTLVAAALLALLVGAIVGSAITVRRRRRHGPSVYAAVARRIAE
                     RPAGDGAGGASR"
     gene            complement(6114..7745)
                     /locus_tag="VEO00_06935"
     CDS             complement(6114..7745)
                     /locus_tag="VEO00_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57768.1"
                     /translation="MPRLARASLGAVLGASVLAAPAVPARSTVPAAVSAAVGAVDLSS
                     FMGLGSWVDIYDHRSFADPEGTVAAMAAAGVRTLYLQTSNFRAPGALMYRERLGRFLD
                     AAHARGMATVAWYVPALTDVTVDLNRVLAAVGFRSPTGVAFAAFGLDIEAREVADPAA
                     RNRRLLALSGRMRRRVGPDYPLAAIVFPPAQMEMFPDFWTGFPYRGLAETYDVFMPMG
                     YWTFRVEGRGAAYHYTRRTIEILRTETGRPDVPIHAIGGIADDAEPAEVTGFVRAVRE
                     HGLIGASLYDFATSDARDWGLMAQIAPNPVQAHTLPLAIDEDVRGFGDFAGDDTHPRE
                     VIFRLGRRPGTWELDFQGLGEGLGEVTVYVNWEPVARLEPAESGWSGRRTVALPPEAL
                     DRGSNLVAFVVEATGPPWPEWGVRQVRLVPPTLPRPDRSPHGNVPGGETSRFDRVSYR
                     LPEGAGPLALTVSAFGTAPGEVAIDVNGRRVGTLARSGGRRWSGPQLVVLPRGALHGD
                     GTDALTFDAAGRPGTRPVWGVRLLSLRGLTPFAVL"
     gene            7807..>8623
                     /locus_tag="VEO00_06940"
     CDS             7807..>8623
                     /locus_tag="VEO00_06940"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HYU57769.1"
                     /translation="MPVVVTGAGGMVARRLLPRLVAAGSEIRALVRRPEQVEVIRVAA
                     PGTKVAVADLSDVENLAWALHDAHTVVHLAGGLDQLTTEAYETSNLRTAEWTLEAAER
                     AKVRRFLFLSYTGASRAATNPYLRAKGQAEEAVANSGLDWLVLRSTHVYGQGSAWLDA
                     MVGLARRRPALVVGPGTQRLAPVFVDDVAAALAAADDREAPLTGTYAIAGPDEISADD
                     LTDRLAARRARKVHLSPRVAARLSRLARRPASVTLIEVMAADSLPDAPDARTPL"
BASE COUNT         1263 a         3264 c         3059 g         1037 t
ORIGIN      
        1 cgaggatctc gcgatcgtag gaccaggcga actgtgggag cgtccgccgt cgcggacctc
       61 ccgaggggag cgccccggag aagcgggaga gccactgcac ccgcgccgcc agcgtgcgcg
      121 gcgtggcgca ggtgaccgcg ccggccacca ccgggtgccc ggcccggtgc cagcccaccc
      181 tccgccccac ccagtcggga tccgggatgc aatccgccgc caggaacagc aggtagcgcc
      241 cccgggcgcg ggccatgccc gcgttgcggg ccgccccggt ggtgagcccg acgggggagg
      301 tcaccacctc gagctcgaac gcccaggtac cgagtgttgc caacgcggcg ccgcctccat
      361 cgtcgctcga gtccaccagc accacttcga acggttcctc ggtgcgctgc ccgagcaggc
      421 cctcgaggac gcggccgagg gtgccgtggt cgttgcggca gaggacgacg accgatgcgg
      481 ccggcacggg cccgtccacc taccgcgcgc gctcgagcgc tcgggccacg tgtgcggcca
      541 tctcgtcgac ccgctcgtcc caggtcggtg ccccgcgccc cggcgggcgg cggccctcgg
      601 caagcgcctc ggccagcgcc tcggcgaacg cgtccgcccc cgtcgccagg cggacgggcg
      661 gggcgaacgg ccggacctcg gggacgtcca ccgacaccac cggcaggcca tgcgccaggt
      721 actcgcgcag cttgaccggg ctcgccgcgg tcgtggccgg ggtgacccgg tacgggatga
      781 tcccggcgtc gaacgctcgc aggagcgccg ggatctcctc atgcggccgt tcgccggtca
      841 gcaggacgtt gtcgcgggcc cgcaggccgt tgacccggat ccgcaccggc ccgaccagga
      901 cgaacgacca gtcggggcgc gacgccgcga gctcctcgac cagtcccacg tcggtccact
      961 cggtgatgct cccgacgaac ccgatccgtg gatgggggag gcgatcgacg gcatgcggga
     1021 cgcgcgtcgt cgagcgtggc gaatcgggag cgccgtgggg gatcaggtgc gtctcggggt
     1081 tcccgccccc gcggcggcgc aggagctcgg cggaggccac gaacaccagg tcggccgcct
     1141 tcgccatggc cgtctcccac ccggcgatgc gccggcgcac ggccgggctc gccgtgagga
     1201 cgtgcgcgga ggcgtcgtcg gaccagtcgt agaccaagag gtcggcgccg agcccggcgg
     1261 ccgcgtcgcg gatcaccggc atcggcaggt acgtccagac cacgagcggc cgcccgccac
     1321 cgacccggcg gcgcagccgg tggagcagcg tccaccgccc gaacgcgcgc accagcgcga
     1381 ggtgttgcag gggcaggacg accggagcgt cgcgcaggat ccggccagca ccaccggggc
     1441 gctccgtggt cggcgtccac gacgatcgcg cccagcggct cacgcggtgg gccacccgcc
     1501 gagcgtcgga gatccgcggc agtcgggtgc cgaggttgtc cacgtacagc acgctcgccc
     1561 cgcggtcggc gaacccctgc atgagccatt gcggccgctg tcggtgggag tcccagtcca
     1621 cccccgcgaa gcacacgatg tcccagggcg tgttcatacc gatccgggcg gatggtatcg
     1681 catgacctgc gggcctccgg tcccggggct cgcgatcagc gaggcggcta caggccggcc
     1741 aggcggcgca ggccggccac gtctttcagc acgtagcgcc ggccgctacg ctcgaccagg
     1801 ccgcgtcgcg ccagctccga gaggatgcgc gtcacgttct cccggctgcc accggacagc
     1861 gcggcgaggt ctgcgtgcgt gatctccgac gggacgatcg ccccgtcctc ggggatctcc
     1921 tccaagctgg gcaccaccat gccgaggagc cgcttggcga cgcgcccctt caggtcaagg
     1981 aacagcaggt cctcgacgtg cgcctccatc gcggcgatct gccgggccag ggccttcagc
     2041 agcgccagcg tcgcccgcgg ctcgcgctcc aggaacccca ggaagtcggc gctgccggtg
     2101 acccacacat cgctctcctc cagcgcgatc gccgtcgcgg tgcgcggcat gtgcccgagc
     2161 acgccgaact cgccgaagaa ctccgggtgg tcgagcgcgg tgatcagctg gcggtggccg
     2221 gtggacgaca gcgtgccgat ctcgatccgg ccggcgacca ggaagaagac ctcatcggcc
     2281 gggtctccct ggtggaacag gtacgttccg cgccggtagc tgcgcagccg tccgctcccg
     2341 gccagtccct cgaccgccgg gcgggccagg tcggacagga gcggcgtgcc ggccatcgtc
     2401 gtgacgaccg atcggcgcgc cgcgggtgcg agcgaggtgg gcacgttcgc gagggtagac
     2461 cacagcgtcg gcgcggcgcc agacgcccag gagccggacg cgaaggacgg gcccggaaag
     2521 gcccgccctc gacgttcggt tggagacgga taggttcagg acctgctcgc ccggctggcc
     2581 cggaccgccc gcgacgcgcg cacggccctg gtggcccgca cggcccgcgt cgcccgcgtg
     2641 gccctggtcg cgcgcgtagc gcgaaccgcc ttcacctgct tggccgcctt cgacgccccg
     2701 ctcttcgcgg tgccgcccga gggcttcttc ggagtgacct tcttctccgc cattcctgct
     2761 caccccctcc gtcaggctcg atgactcgat gaccaaagtg tggaccgggc cgcctcggag
     2821 cgatgtgccg tgcgtcaccg gatcggatgt gacgtgcgac acccccgaaa tgactactct
     2881 tcccccgttg ggggtccgcc tcgccgtaca cggtcgggcc gatccgtccc ggggcgacgt
     2941 gcgcccccca aacctcccga atcgtcgtcc atgtccagct ccgggaggtc gatcggatcg
     3001 ggccgcggag tgcacgcgcc gcagtacagc gcgtcctcga ccacgagcgc gcgcgggacg
     3061 cccgccgagc ggtacagctc caggacgtag gggtgcgtca gcgcgtggtc ctcgcacacg
     3121 ccgcgtccgc agaagcggca cgtccccacg gccgtcctcc ggcagtgcca acagtccacg
     3181 gtccctcctt accccttccg aaaccccgtc acgacgggcg ctcggacggc atgtgcgggt
     3241 cgagggtgcc gcggcggaac gagcgggcga tcagccggtc gaacccctcc cgtcccagcc
     3301 ggaaggcccg caccggcgtg agcgtgacga cggtggccgt ccgcgggaca tccatcagca
     3361 gcgccacctc gccgaagtag tctccgcggc cgagcgtcgc cttccgctcc ccgtcgatcc
     3421 gcacctcgac ctggccggag ccgatggcgt agaacgcgtc accggtctcg ccctgctcca
     3481 tgaccacctc cccgggcggc atgtccgtcc actcgccctg ggccagcacc tcggacgcct
     3541 ccgacggctc caggtgggcg aatgcgcgca gctcgcgcaa ctcgatcgtc tcctggagca
     3601 ccggggcgaa cgacggcacc ttgatcatgt cggccagcag gcggtcgaac gtgctctcgt
     3661 cgatctggaa gatctgggac ggctcgagcg cccggacggt caccgcccgc ggggtcccgc
     3721 gcagcaggcc gagctcgccg aacgactcgc cgcgcccgag cttgcgcagc acgcgccgtg
     3781 cccccgtctc agggttctcc tcctcgacct gcagggtgcc gtggcggacg acgtagaacg
     3841 cgtcggcccg gtcgccctgc cgaacgacct cgcgatcgcg ggccaccgtg tgcagccgga
     3901 cccgcccggc caggtcgctc aggacgtcct cgggcacgtc gtcgaacagc gggagcgcgt
     3961 cgatcagcgt ggcggcctcg acgcgccacc cggtctgcag gtggaaccgg atgcggtcgg
     4021 ccaccgcctt cacccgcagc cacagcgagc gcaccaggcc gaccagcccg cggatcaggg
     4081 gtcccccgac gaacaggccc agggcgatca ggatcagccg cgtcgtcgtc cctcccgccc
     4141 acagccggcc gaccaggccg ccgaacaccg cctcccagaa gaagtaggcc gtgtagaacg
     4201 agaagatcgt gaacgtgatg ccgacgacgc cgtagagtgc caggccggcc tcccgcgagg
     4261 tgaagcgctc gcggcgccac agcttgcgcg cgaggtcgtg gcgtacgaac gcgatcgagc
     4321 gcgggcgcag gtcggggatc tgcagggcgt ccgcaaggat ccagtacccg tccagctcga
     4381 gcatcggcag gctgttcatg aacacggcga gatacgccag gaccgtgaac ttgtagagca
     4441 gggcagagat cgcgccggca gggaccaggt agacgtagat cgaggtcgcg ccggcgacga
     4501 gcagcccggc gaacggcccg gcggcggact ggatgatccg cttgcgccgt tccagcatca
     4561 gcccgtcgga ggactcgatg tagaaggccg gcgcgccgaa gaagatgaag aagccggcgg
     4621 ccttgacctt ccgcccgtag tgcacgagca cggacccgtg tcccagctcg tgcaggaaga
     4681 tgtggaacag ctgcaacagt aggagcaaca agaagccgat cgccagcgac tgaccggcaa
     4741 ggtcgaagcg gctcgagcga acgttggcga agaacgccac cagtcccgcc agcgcgacga
     4801 gcaccgacag cgccatcgcc ggcctggtga acagcacccg caggccgcgc cggtagagcc
     4861 agcggacgaa gcggtcggcg ttcccccact cgagccgcag atcggtgacg aactcccgcg
     4921 tcttgcgccc gaccgtggtc tcggggttga gcgccttgtc gatcatgccc tcgacgtcgg
     4981 tgaagtgcga ctgcaggaag ttgccctcgt gcagctggcg aaccaggtcg gcgacgccgt
     5041 cgagctccat ctcgccggat tccctgaagc gctcgacgac gatctccttg accgtgcggg
     5101 tgccgtccat caggggcagg agctccacct ctccggcctc gaggcggtaa tgcagcatct
     5161 cgcgagggtt ggccgccatc gcgtagccct cgccccagcg cgtcgggaag ctcttcacct
     5221 cgatgtcggg ggcgagcgcc gggcggaacg ccccagggtc ggcccgctcg gcgagcgtca
     5281 cccaggtccc ctcgtcgcgc tcgaggtcgg tcggctgccc ggcgaccgcg tcggccaggc
     5341 cctcccacag cccgccgggg ggtccgctca tcgggacgcc ccgccggcgc cgtcccctgc
     5401 cgggcgctcg gcgatccggc gggccacggc ggcgtagacc gagggaccgt gccgccgccg
     5461 gcggcggacg gtgatcgccg accccacgat cgcgccgacg agcagcgcca gcagcgccgc
     5521 ggcgaccagg gtcccgaccg acacgccgcc acgcggctcg gggatgcgtc gcaacaggac
     5581 cgtccgcacc ggcacgcccg gcggtccggc cgccgtcagc accagccggg acccggggga
     5641 ggggtccgcg gcgaaccgga tcgtggcggt cacccggacc cgctgcccac cggcggcgat
     5701 caccactcgg gacacgtcga ccacgaagaa ggcgccctcg atcacgccct cggttccggc
     5761 gatctgcgcg gcgccctcgc cggcgatcgc gaccaccgcc tgggccggat ccaccaccag
     5821 acggtcgagc gaacggccgc ggagccggag ctccacggcg atctcccgaa cgtcctccca
     5881 gcccgccggg tcgatcgcct cggcggtcac ggtgagcagg ccgccgtcga cggtctcacc
     5941 gacggcgtcg ccggagatcg tgacgccgcc ggcctgtgcc accgccgccc gcgcggcgaa
     6001 gccaggcacg accgatcccg ccaggacgaa gagcgcaccc caggcaaccg tccgatgcat
     6061 gagccgacgg gagtgtaggg gccgaccctg ccgcgatgga accccgcggc ccctcagagc
     6121 accgcaaacg gggtcaaacc acggagcgac aggagccgca cgccccagac cggccgggtt
     6181 ccgggacggc cggccgcatc gaacgtcagc gcgtcggtcc cgtctccgtg cagggcgccc
     6241 cgcgggagga ccacgagctg cgggccgctc catcgccgcc ctccggaacg ggccagcgtc
     6301 ccgacccgcc gcccgttcac gtcgatggcc acctcgccgg gcgcggtgcc gaacgccgac
     6361 accgtcaggg ccagggggcc ggcgccctcc ggcagccggt agctgacccg gtcgaatcga
     6421 gaggtctcgc cgccaggcac gttcccgtgc ggcgaccggt ccgggcgggg cagggtgggc
     6481 gggacgagcc tcacctggcg caccccccat tccggccagg gcgggccggt cgcttcgacg
     6541 acgaaggcca ccaggttcga accgcggtcg agggcctcgg gcggcagcgc cacggtccgc
     6601 cggccgctcc agccgctctc ggccggctcg aggcgagcca ccggctccca attgacgtag
     6661 acggtgacct cgccgaggcc ctccccgagg ccctggaagt cgagctccca ggtgcccggc
     6721 cggcggccga gccggaagat cacctcgcgc gggtgggtgt cgtcgccggc gaagtcgccg
     6781 aacccgcgta cgtcctcgtc gatcgccagg ggcagcgtgt gggcctggac cgggttcggc
     6841 gcgatctggg ccatcagtcc ccagtcccgc gcgtcgctcg tggcgaagtc gtacaggctg
     6901 gccccgatca gcccgtgctc tcgcaccgcg cggacgaagc ccgtgacctc ggccggctcc
     6961 gcgtcgtccg cgatcccccc gatggcgtgg atcgggacgt cgggacggcc ggtctcggtg
     7021 cgcaggatct cgatcgtgcg gcgcgtgtag tgataggcgg cgccccgccc ctcgacgcga
     7081 aacgtccagt accccatcgg catgaacacg tcgtaggtct cggccaggcc ccggtacggg
     7141 aaccccgtcc agaagtcggg gaacatctcc atctgcgccg gcgggaagac gatcgccgcc
     7201 agtgggtagt cggggccgac ccggcgccgc atgcgccccg agagggcgag cagccgccgg
     7261 ttgcgcgccg cgggatcggc cacctcgcgc gcctcgatgt ccagcccgaa ggcggcgaag
     7321 gccacgccgg tcggcgaccg gaacccgacc gccgcgagca cgcggttcag gtcgaccgtc
     7381 acgtcggtca gcgccggcac gtaccaggcc accgtggcca ttccgcgggc atgggccgcg
     7441 tcgaggaacc ggccaagcct ctcccggtac atcagcgcgc cgggcgcgcg gaagttcgag
     7501 gtctgcaggt agagcgtgcg cacgccggcc gcggccatcg cggccaccgt cccctccggg
     7561 tcggcgaacg agcggtggtc atagatgtcc acccaggagc cgaggcccat gaaggacgag
     7621 aggtcaacgg cgccgacggc cgccgacacg gcggcgggca cggtggagcg ggcggggacg
     7681 gccggagccg ccaggacgct cgccccgagc accgcgccca gagaagcgcg cgcgagccga
     7741 ggcacgggaa tcaccttcga cgcatgctcc cacacctcct ccgcgctggc ccggtagcct
     7801 cgaagaatgc cggttgtcgt gacgggcgcg ggcgggatgg tggcgcgccg cctgctcccc
     7861 cgcctggtcg ctgcgggctc ggaaatccgg gcgctggtcc gccgaccgga gcaggtcgag
     7921 gtgattcggg tggcggcccc cgggacgaag gtcgcggtcg ccgacctctc cgacgtggag
     7981 aacctggcct gggcgttgca cgacgcgcac acggtcgtgc atctcgccgg ggggctcgac
     8041 cagctgacca cggaggccta cgagacctcg aacctgcgga cggccgagtg gacgctcgag
     8101 gccgccgaac gggcgaaggt ccgccggttc ctgttcctct cgtacacggg cgcgtcgcgg
     8161 gcggccacca acccctacct ccgcgcgaag ggacaggccg aggaggcggt ggcgaactcg
     8221 ggcctggact ggctggtact gcgttccacc cacgtctacg ggcaggggag cgcctggctc
     8281 gacgcgatgg tcgggctcgc ccggcgccgc ccggccctcg tggtcgggcc ggggacccag
     8341 cgcctggcgc cggtgttcgt ggacgacgtc gccgccgccc tggccgccgc cgacgaccgg
     8401 gaggcgccgc tgaccggcac ctacgccatc gccggccccg acgagatctc cgccgacgat
     8461 ctgaccgacc gcctggccgc ccgccgagcc cgcaaggtcc acctctcccc gcgcgtggcg
     8521 gcccgcctct cccgcctggc gagacggccg gcctcggtca cgttgatcga ggtcatggcc
     8581 gccgactccc tccccgacgc ccccgacgcg cgcacaccgc tcc
//
LOCUS       DASPWO010000300         4340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_813065, whole genome shotgun sequence.
ACCESSION   DASPWO010000300 DASPWO010000000
VERSION     DASPWO010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813065"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="VEO00_06945"
     CDS             <1..1638
                     /locus_tag="VEO00_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HYU57770.1"
                     /translation="WIILLFLLLIAAIVGFNARATASERDTVLTINVAARQQALAERY
                     IKDVLLTVEGFPADPQQDATMLADTALALLQGGEVLAVQGADTTVLIQPVQSDWRSVA
                     KLQQAGKLIDKLIATGDSLLVLSRDDPGFDQQLLQLRIVGAQVSSITNDAVGEMTHQA
                     QASLWHLVRIGVGLGLIGAVAAIAMALFLRRAGIQQSAQFRSLVQNASDLVTVLDGAG
                     KVQYQSASAESILGVPSSEFLGRNISELVHADDAEAVSETIQDLLGRPGATARIEYRL
                     VGADGAPRHVESAVTNLLSEPAVQGLVLNTRDMTKRHEADEKLRQLQDERSGLLQQTV
                     HATELERKRVAAELHDGPVQHLTAFDVRLESLRARVERADTAGAAGLLDQLQERLRKE
                     VQELRKMLVGLRPPMLDERGLPAALLEHLTAVERDAGIRCSLESALDTRLSLAQEVIL
                     YRVAQEALTNVAKHARASHVWVSLGESDGRVTLLVRDDGVGFDPSRVAESTRNGHFGL
                     LGMRERVEMAGGTWAVAAGPDGGTLVSADVPREVGAS"
     gene            1635..2063
                     /locus_tag="VEO00_06950"
     CDS             1635..2063
                     /locus_tag="VEO00_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU57771.1"
                     /translation="MNATAERPGKTSIPRVLVVDDDDVLREALCMLLTERGIDVVGEA
                     GDGFAGVELARERHPDVVLIDFRMPGMDGLQATNLIKAEEPLIQSVVFTAYDDQTLDL
                     EAARAEVYCLLVKGCRPQLILDMLHGAAMRKWELETGQPQ"
     gene            2115..2975
                     /locus_tag="VEO00_06955"
     CDS             2115..2975
                     /locus_tag="VEO00_06955"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HYU57772.1"
                     /translation="MEHKRFGAQSMTSRLREVLVKRPGLAFGRAFDDERNGFLHPVEL
                     ERARRQHDRLCELLAGLGVTVHELGVDTGGPDLVYTFDPAIVSDDGVIPLRSGKPTRL
                     GEETVIERWATDREIPVAGRIEAPGTVDGGDTLWLRPDLFCIGRSLRTNQIGATQLEG
                     IVGGDVRVFDVPYANGPDECLHLLSVISPIADDMVVVFLPLLPAGLHEVLGDLGVSLI
                     PVPDDEFATLGCNVLAVSPGVVVVADGNPVTRRALAERGCEVHSFEADEVGLNGSGGP
                     TCLTRPILRD"
     gene            3078..3821
                     /locus_tag="VEO00_06960"
     CDS             3078..3821
                     /locus_tag="VEO00_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYU57773.1"
                     /translation="MLDMATREILDLSAGGTVSQLVPSLPGEVVREAAWAARCAGHID
                     AVLGAELDLDDWDLYGRRRDLAWGEPLFSEGRPAKGVWIVRDGEVELFVGSGRARQVV
                     EVLGPGGIDGDIQSLLGLRLAYSARAATDLSAWFLAIQSFRQLLSDRPVLAGRWLTSV
                     ASRIANSHRRIVALLGQSLTVQVARLLRDEAIDREVHLPQRTLAAMLGVQRPSLNKVL
                     KELEKRGLVDIGYGRITITDPAGLTQLAD"
     gene            4037..>4340
                     /locus_tag="VEO00_06965"
     CDS             4037..>4340
                     /locus_tag="VEO00_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HYU57774.1"
                     /translation="MNRFPEAVATGDFDADGDSDAAYARSDFFDQGMTVQLNSATGPW
                     GQAASYPATDESADIKAGDLDTDGDLDLAVVSMGSSFTNDVIDLYVNDGQGRFVHRT"
BASE COUNT          676 a         1476 c         1535 g          653 t
ORIGIN      
        1 tggatcatcc tgctcttcct gttgctcatc gcggccatcg tgggcttcaa cgcccgggcc
       61 accgccagcg agcgcgacac cgtcctgacc atcaacgtcg ctgcacggca gcaggcgctg
      121 gccgagcgct acatcaagga cgtcctcctc acggtcgagg gcttcccagc ggatccccag
      181 caggacgcca cgatgctggc cgacacggcg ctcgccctgc tccagggcgg ggaggttctg
      241 gcggtccagg gcgccgacac caccgtcctg atccagcccg tccagtcgga ctggaggtcc
      301 gtggccaagc tccagcaggc gggcaagctc atcgacaagc tcatcgccac gggcgactcg
      361 ctgctcgtcc tgagccggga cgatcccggg ttcgaccagc aactgctgca gttgcggatc
      421 gtcggcgccc aggtctcgag catcacgaac gacgcggtcg gagagatgac ccatcaggcc
      481 caggcctccc tgtggcacct ggtgcggatc ggggtcggcc tcggcctgat cggggcggtg
      541 gccgcgatcg cgatggccct gttcctgagg cgggccggga tccagcagtc ggcccagttc
      601 cgatccctgg tgcagaacgc ctccgacctg gtcacggtgt tggacggcgc ggggaaggtc
      661 cagtaccaga gcgcgtccgc cgaatccatc ctgggggtgc ccagctcgga gttcctcggc
      721 cggaacatct ctgagctcgt ccatgccgac gacgcggagg cggtctccga gacgatccag
      781 gacctcctgg gccgtcccgg cgccacggcc aggatcgaat atcggctggt cggggccgat
      841 ggcgcccccc ggcacgtgga gagcgcggtc acgaatctgc tgtccgagcc ggcggtccag
      901 ggactggtgc tgaacacccg ggacatgacg aagcggcacg aggccgacga gaagctccgg
      961 cagctccagg acgagcggag cggcctcctc cagcagacgg tccacgcgac cgagctcgag
     1021 cgcaagcggg tcgcggccga gcttcacgac ggtccagtcc agcacctcac ggccttcgac
     1081 gtgaggttgg aatcgctccg agcccgtgtc gagcgcgccg ataccgcggg cgcggcgggt
     1141 ctgctcgatc agctgcagga gcggctccga aaggaggtcc aggagctccg caagatgctc
     1201 gtcggccttc gccctccgat gctcgacgag cggggcctcc ccgcggcgct gctggagcac
     1261 ctcaccgccg tcgagcgaga cgcaggaatc cgctgctcgc tggagtccgc cctggacacc
     1321 cgactctcgc tggcccagga ggtcatcctg tatcgcgtgg cccaggaggc gctcacgaac
     1381 gtcgcaaagc acgctcgcgc ttcacacgtc tgggtgtcgc tcggggagtc cgacggccgg
     1441 gtcacgctcc tggtccgcga cgacggcgtg gggttcgacc cctccagggt ggccgagtcg
     1501 acgcggaacg gccacttcgg cctgctgggc atgcgggagc gcgtggagat ggccggggga
     1561 acttgggcgg tcgcggccgg cccggacggt ggaacactcg tgtccgcgga cgtcccgaga
     1621 gaggtgggtg catcgtgaac gcgacggcgg aacgacccgg gaagacctcc atcccgcggg
     1681 tgctggtggt cgacgacgac gacgtgcttc gagaagccct gtgcatgctg ctcaccgaac
     1741 gggggatcga tgtggtcggc gaggccgggg acgggttcgc gggggtcgag ctggcccggg
     1801 agcgacaccc cgacgtggtg ctgatcgact ttcggatgcc cggtatggac ggcctgcagg
     1861 ccaccaacct gatcaaggcg gaggagcccc tcatccagtc cgtggtgttc accgcgtacg
     1921 acgaccagac gctcgacctg gaagcggccc gggctgaggt ctactgcctg ctggtgaagg
     1981 ggtgccgccc gcaactgatc ctggacatgc tccacggggc ggccatgcgc aagtgggagc
     2041 tggagaccgg ccaaccccag taacctgctc gtcgtgcccg gggcctccgg acggctcccg
     2101 cgcagccgaa gcccatggag cacaagcggt tcggagccca gagcatgacc tcgcgattgc
     2161 gcgaggtgct ggtgaagcgc ccggggctcg cgttcggccg agccttcgac gacgagcgaa
     2221 acggtttcct ccatcccgtg gaactcgagc gggcccggcg ccagcacgac cgactctgtg
     2281 agctcctggc ggggctcggg gtcaccgtcc acgagctggg cgtcgacacg ggcggcccgg
     2341 acctggtgta cacgttcgac cccgcaatcg tctcggacga cggcgtgatc ccgctccgct
     2401 ctgggaagcc cacccgtctc ggcgaggaga ccgtcatcga gaggtgggcc acggatcgtg
     2461 agatcccggt ggccgggcgg atcgaagccc cggggaccgt cgacggtggg gacacgttgt
     2521 ggctgcggcc cgacctgttc tgcatcggca gatcgcttcg cacgaaccag atcggggcga
     2581 cccaactgga agggatcgtg ggcggcgacg tccgcgtctt cgacgtcccg tacgcgaacg
     2641 gcccggacga gtgcctgcac ctgctgagcg tgatctcgcc gatcgcggac gacatggtgg
     2701 tggtgttcct gccgctcctc ccggcgggcc tgcacgaggt gctcggcgat ctcggggtct
     2761 cgctgatccc cgtccccgat gacgagttcg ccacgctcgg atgcaacgtg ctggcggtct
     2821 cgcccggcgt ggtggtggtg gccgatggca atcccgtcac gcggcgggcc ctcgcggagc
     2881 ggggctgcga agtccactcg ttcgaggcgg acgaggtggg gctcaacgga agcggaggcc
     2941 ccacctgcct gacccgcccg atcctccgcg actaagtcct gcaccgcctg gccgggagcc
     3001 tccgttcgcc tcgggcggaa tcagatcgca ccggcgggcg tcccactggt gacgggcggt
     3061 cgtgacgacg ctgggggatg ctcgacatgg cgacgcggga gatcctcgat ctgtcagccg
     3121 gaggaacggt cagccagctc gtcccgagtc tgccgggcga ggtggtcagg gaagcggcct
     3181 gggcagcccg atgcgccggc cacatcgacg ccgtgctggg cgcggaactc gacctggacg
     3241 actgggacct ctacggtcgg cggcgcgatc tcgcctgggg cgagcccctc ttctcggagg
     3301 gccgccctgc caaaggcgtg tggatcgtcc gggacggcga ggtcgagctg ttcgtgggat
     3361 cgggccgcgc gcgccaggtg gtcgaggtgc tggggcccgg aggtatcgac ggtgacatcc
     3421 agtcgctgct cggccttcgg ctcgcctatt cggcccgggc cgccacggac ctgtccgcct
     3481 ggttcctcgc catccaatcg tttcgccagc tcctgagcga ccggcccgtc ctggccggcc
     3541 gatggctgac gtccgtagcc tcgcggatcg ccaacagcca ccgacggatc gtcgcgctgc
     3601 tcggacagag cctcaccgtc caggtcgcgc ggctgctgcg ggacgaagcg atcgaccggg
     3661 aggtccacct cccgcagcgc accctggccg cgatgctggg cgtgcagcgt ccgtcgctga
     3721 acaaggtcct caaggagttg gagaagcgtg ggctggtgga catcggatac ggccggatca
     3781 cgatcaccga tcccgccggc ctcacccagc tcgcggactg aacctccccg ccggacccca
     3841 gtcctgagcg gcgttgcgcc tggacgtcac atcatacgga gatccgaagt ttggccgttc
     3901 gatcggccgg aaagcgtggc gcgtacatcg aggggcatcg gacacatcgc ttaccggtcc
     3961 tgtggccgag gcaaacgacc gctcccacga ccgggccggg agacggcacc ccgtttacca
     4021 cgtgggcggc tatcccgtga accgatttcc cgaggccgtg gcgacggggg acttcgacgc
     4081 cgatggcgac agcgacgccg cgtatgcccg gagcgacttc ttcgaccaag gcatgaccgt
     4141 ccagctcaac tcggcgacgg gaccatgggg tcaggcggcc tcgtatcctg caacggacga
     4201 gtccgccgac atcaaggcgg gagacctcga cacggacggc gatctggatc tggccgtcgt
     4261 cagcatgggc tcgagcttca ccaacgacgt aatcgacctg tacgtgaacg acgggcaggg
     4321 acggttcgtc caccgaacgg
//
LOCUS       DASPWO010000301         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_894024, whole genome shotgun sequence.
ACCESSION   DASPWO010000301 DASPWO010000000
VERSION     DASPWO010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894024"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VEO00_06970"
     CDS             complement(<1..993)
                     /locus_tag="VEO00_06970"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF009810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HYU57775.1"
                     /translation="MTSIEVLPIEGLPEVGARDDLADLIAAAPGAPAMRDGDVVVVTQ
                     KAVSKAEGRMVPERLGGRAGWVARESVRTVARRGDLVIAETRHGFVCANAGVDASNVP
                     AGFLTLLPEDPDASAARIRDGLRRHTGAEVAVVVTDTFGRPWREGLVNVAIGVAGLPA
                     VVDLRGSPDGGGRTLEATVVALADEVAAAAGLVLGKAARVPAAVVRGLGWTAPPGTGR
                     DLVRAQADDLFRESPLQAIHARRSVRTFGEGPVPRTALEEAVHAACTAPAPHHARPWL
                     YVVLESAPARRRLLGAMAAAWAADLRADGISEDVAARRLARSDALLGAAPVLALP"
     gene            complement(1010..1942)
                     /gene="cofD"
                     /locus_tag="VEO00_06975"
     CDS             complement(1010..1942)
                     /gene="cofD"
                     /locus_tag="VEO00_06975"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HYU57776.1"
                     /translation="MNVTALAGGVGAGKFLRGLVRAVPAEHVTVIVNTGDDLVVNGLR
                     VSPDLDSVTYWLAGVADRERGWGRDGETWRALEELRRLDAEAAWFALGDLDLASHQLR
                     TRLLAEGRTLSEATEAVRRGYGVRTRLLPMSDDPVTTRIETADGPVLDFQDYWVRRGA
                     RDAVAGVSYDGAANARPAPGAIEALAGADAVVICPSNPVASIGPILAIPGFREALETR
                     AGTVAGVSPIVGGAPLRGMADRLMPAAGLEVSAAGAARAYAGLLGRWVIDEADRALEP
                     AIAATGLRVAVTDTIMVDDAAAERVARAALDLAS"
BASE COUNT          253 a          845 c          728 g          204 t
ORIGIN      
        1 gggcagcgcc aggacgggcg cggcgccgag cagcgcgtcc gagcgcgcca ggcggcgggc
       61 ggcgacgtcc tcggagatcc cgtcggcgcg caggtccgcc gcccaggccg cggccatcgc
      121 ccccagcagg cggcgccgcg cgggggcgga ctcgagcacg acgtacagcc acggccgggc
      181 gtgatggggc gccggcgcgg tgcacgcggc gtgaaccgcc tcctccaggg ccgttcgcgg
      241 gaccgggccc tccccgaagg tgcggaccga ccggcgggcg tggatggcct gcagcggcga
      301 ctcccggaac aggtcgtcgg cctgcgcgcg gaccaggtcg cgccccgtgc ccggcggagc
      361 cgtccacccc agcccgcgga ccacggcggc cgggacgcgc gccgccttcc ccaacaccag
      421 ccccgcggcg gcggcgacct cgtcggccag cgccaccacg gtcgcctcca gcgtcctccc
      481 gccgccgtcg gggctcccac ggaggtcgac caccgccggc aggccggcga ccccgatcgc
      541 cacgttcacc aggccctcgc gccagggccg cccgaacgtg tcggtgacca cgacggcgac
      601 ctcggcgccg gtgtggcggc gcaagccgtc gcggatccgg gccgccgacg cgtcggggtc
      661 ctcgggcagc agcgtgagga accccgccgg gacgttcgag gcgtccaccc ccgcgttggc
      721 gcacacgaag ccgtggcgcg tctcggcgat cacgaggtcg ccgcggcggg ccaccgtgcg
      781 caccgactcg cgcgccaccc atccggcccg ccccccgagc cgctcgggca ccatgcggcc
      841 ctccgccttc gagaccgcct tctgcgtcac cacgaccacg tcgccgtcgc gcatcgccgg
      901 cgccccgggc gcggcggcga tgaggtcggc caggtcgtcg cgggcgccaa cctccggcag
      961 cccctcgatc ggcagcacct cgatcgaggt cacgacggga ccgccacgct cacgacgcca
     1021 ggtccagggc ggcgcgggcg acgcgctccg cggccgcgtc gtcgaccatg atcgtgtcgg
     1081 tgaccgccac gcggagcccc gtcgcggcga tggccggctc gagcgcgcgg tcggcctcgt
     1141 cgatcaccca ccggccgagc agcccggcgt aggcccgggc ggccccggcc gccgagacct
     1201 ccagccccgc cgccggcatc agccggtccg ccatcccgcg cagcggcgcc ccgccgacga
     1261 tcgggctgac gccggccacg gtcccggccc gcgtctccag cgcctcccgg aaccctggga
     1321 tcgccaggat cgggccgatg gaggccaccg gattcgatgg gcagatcacg acggcgtcgg
     1381 cgccggcgag cgcctcgatc gccccgggag cggggcgggc gttcgccgcg ccgtcgtagc
     1441 tgacgccggc caccgcgtcg cgcgcacccc gccggaccca gtagtcctgg aagtcgagaa
     1501 cgggtccgtc ggcggtctcg atccgcgtcg tcacggggtc gtcgctcatc ggcaggaggc
     1561 gcgtgcggac cccgtacccg cgccggacgg cctcggtcgc ctccgagagc gtccgcccct
     1621 ccgccagcag ccgcgtccgc agctggtgcg acgccaggtc gagatcgccg agcgcgaacc
     1681 acgccgcctc cgcgtcgagc cgccgcagct cctcgagggc ccgccacgtc tcgccgtcgc
     1741 gaccccagcc gcgctcgcgg tcagccaccc cggcgagcca gtaggtcacc gagtcgaggt
     1801 cgggggagac ccgcagcccg ttcacgacga gatcgtcgcc ggtgttcacg atcaccgtga
     1861 cgtgctccgc gggaaccgcg cgcaccagcc cgcgcaggaa cttccccgcg ccgaccccac
     1921 cggccagggc ggtgacgttc atccggacgc gccgttcacg atggaatcac cgcgaagagc
     1981 cgccactcgc cgggcacgcc cttcagcccg tggctgccgc ggtcctcgaa
//
LOCUS       DASPWO010000302         3489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1146209, whole genome shotgun sequence.
ACCESSION   DASPWO010000302 DASPWO010000000
VERSION     DASPWO010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146209"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..218)
                     /locus_tag="VEO00_06980"
     tRNA            complement(144..218)
                     /locus_tag="VEO00_06980"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(184..186),aa:Val,seq:gac)
     gene            complement(224..294)
                     /locus_tag="VEO00_06985"
     tRNA            complement(224..294)
                     /locus_tag="VEO00_06985"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Cys,seq:gca)
     gene            complement(332..688)
                     /locus_tag="VEO00_06990"
     CDS             complement(332..688)
                     /locus_tag="VEO00_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57777.1"
                     /translation="MSARVVDPRLQRPASGWFWVRWAFGALFSFLGVVMIVASLVGIP
                     LALYFFIGLRWFLAVPLWLVTPLVGWLLLRLGRVMRGLTAHGLPRDAGPRPWQTVPEP
                     RSRASVADAGEMGGVL"
     gene            983..1576
                     /locus_tag="VEO00_06995"
     CDS             983..1576
                     /locus_tag="VEO00_06995"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HYU57778.1"
                     /translation="MSEVREYPARLKLEGPLEIKNWRPLVNWLLAIPQLLVAQALRAV
                     RQVLQLIAFFTILFTKRIPRPLFDMIVMTLRYRWRVTSFTLWMRESYPPFDFTPASED
                     PANDPASVTVDYPEELNRWLPLVKWWLLAIPHYIVLFFLYLGAFFVGVVAFFAVLFTG
                     RYPEGMRSYLVGVNRWSLRVLSYAGLLRDEYPPFSLN"
     gene            complement(2219..2776)
                     /locus_tag="VEO00_07000"
     CDS             complement(2219..2776)
                     /locus_tag="VEO00_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57779.1"
                     /translation="MRRSFVVAAAILAALTLLATQALAADLTGTPGNDYLYGTNGNDN
                     INGLAGHDWIWGNGGGDIIIGSYGNDKLIGNDGPDHLYGRSGGDTLWGEELGCGYWVG
                     GLHATGGPVPCPSPDLDQFYGGTNDDVIHADDRAFQPGDPSLGEFIDCGGGYDIAFVD
                     PSDWARALVGQINLLTTCESIFVSL"
     gene            3194..3361
                     /locus_tag="VEO00_07005"
     CDS             3194..3361
                     /locus_tag="VEO00_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57780.1"
                     /translation="MSEIEIAADLFENEHTWDQVDRLAAELPERKTMSGRAARETLLA
                     SLDGRFPVTRS"
BASE COUNT          568 a         1191 c         1183 g          547 t
ORIGIN      
        1 gctggacgtg cgggtggtgt ctgaggacgg cgaactgctc cggcacctga ccctcgaccc
       61 gaaccgcgac taccagcccc aaggatgacc cgggtggcta cgatgtcccg agacatccgt
      121 ccacgatgtc ccgagacatc agatggtggg cgctggagga tttgaacctc cgacctcttc
      181 cgcgtcaagg aagcgctctc cccctgagct aagcgcccgc gtgaggcggg gagcggaatt
      241 gaaccgctgt gcggggtttt gcagacccct gcctaaccac tcggccaccc cgccggggaa
      301 ccatcagtct aggtggaacc ccggacgcct gtcagagtac tccgcccatc tcaccggcgt
      361 cagcgacgga agcccgcgaa cgtggctcgg ggaccgtctg ccacggcctg gggccggcgt
      421 cgcgcggcag gccgtgcgcg gtcaggccgc gcatcacgcg gccgaggcgg agcagcagcc
      481 agccgacgag cggcgtcacg agccagaggg gcaccgccaa gaaccagcgc agcccgatga
      541 agaagtagag cgccagcggg atccccacca gtgaggccac gatcatgacc acgccgagga
      601 acgagaacag cgcgccgaac gcccaccgca cccagaacca gccggacgcc ggccgctgca
      661 gccgcggatc cacgacgcgt gcgctcatgg gggatcagcc tacccgcacg gggaccggga
      721 gcggcacgag ctcgaccctt ccgtcgcgat cggggctctc cattcccgct ggtcagcgac
      781 gccgtgctcc ggagctgacc atgcgggact gtcaccccaa cctgtcaccc acgagctggc
      841 gggcgacccc ctaacccctt gtcgggcgct gtcaggcggg cgcatgatcc tcgcagcgca
      901 cgtcggcgga ggcagcctaa actgcccgag agcgaaggac cccgtgcgag ggatcgacga
      961 ccatccggag agtggagggt agatgagcga ggtacgcgag tatccggccc gcctgaagct
     1021 ggagggtccg ctcgagatca agaactggcg gcccctggtc aactggttgc tggccatccc
     1081 gcagctcctc gtggcgcaag ccctgcgggc tgtccggcag gtgctccagc tgatcgcgtt
     1141 cttcacgatc ctcttcacga agcggatccc ccgaccactc ttcgacatga tcgtgatgac
     1201 cctgaggtac cggtggcgcg tcacctcctt caccctgtgg atgcgggaga gctacccgcc
     1261 gttcgacttc acacccgcgt cggaggaccc ggcgaacgac cccgcatccg tcacggtcga
     1321 ctatccggag gagctgaacc ggtggctccc gctggtgaag tggtggctcc tggccatccc
     1381 ccactacatc gtgctcttct tcctctacct gggagccttc ttcgtaggtg tggtggcgtt
     1441 cttcgccgtg ctgttcacgg ggaggtatcc ggagggaatg cgttcctacc tggtcggagt
     1501 gaaccgctgg agcctgcggg tcctctccta cgccggcctc ctccgagacg agtacccgcc
     1561 gttctcactc aattgagggc gtcgagcgct cgaccgcagt gtcgcgggcc aagctgagcc
     1621 ttgaacggtt gaatctggcc tgaccgcggc cccgtgccgt ctggagggcc gcggcgttct
     1681 gcaggtcgga tgccctatct gttggagcga acgacgggat tcgaaccgcg acccccacct
     1741 tggcaaggct attgagccgg tcctgtcccg tcccggctcg tactgctacg tcctagacgt
     1801 gctggtcaga cgctcaggca ggacacggca gaacgacgcc ggacggggcg ggacggcagc
     1861 agtggcaccc aaaccggcac ccggttggcg ccggacatag tgagaacggc agcacgccag
     1921 gggcttgcgc aggacgccag cggcctgtct ccgaggcact gtcttcgcgt ggagctcgtc
     1981 gccttcccgc cccacgccct cctgatcgtt ggggagcgac tcccgagcag gaggaggccg
     2041 gggtgcggat gcctccgatc agtcggacgg agctcgcgct caaggatcct cgtggccgcc
     2101 gcgatgtgtg ggccggctac tggctgacgt cgcgactctc ggtcacgagc ctcgtcgagt
     2161 ctcctcgttc agcggacggg ggccgttgtc cgggccccgt ccgtcccgtc tcgctcgctc
     2221 agagggagac gaagatgctc tcgcaggtcg tgagtagatt gatctgtccc accagggcgc
     2281 gggcccagtc gctggggtcc acgaaggcga tgtcgtaccc gccgccgcag tcgatgaact
     2341 cgcccaggct cggatcgccc ggctgaaacg ccctgtcgtc ggcgtggatc acgtcgtcgt
     2401 tggtgccgcc gtagaactgg tcgaggtccg gggaggggca tgggacgggt ccgccggtcg
     2461 cgtgcaggcc gccaacccag tacccgcagc ccagctcctc gccccacagc gtgtcgccgc
     2521 ccgagcgccc gtagaggtgg tcgggcccgt cgttgccgat cagcttgtcg ttgccgtagg
     2581 acccgatgat gatgtcgccg ccgccgttcc cccaaatcca gtcgtgcccg gccaggccgt
     2641 tgatgttgtc gttcccattc gtgccgtaga ggtagtcgtt gccgggggta ccggtgaggt
     2701 cggcggccag ggcctgggtc gccagcaggg tgagtgccgc caggatcgcc gccgccacga
     2761 cgaaggatct gcgcatcgaa cctccccttc cccatcggcg gccggcggcc gccctatgcg
     2821 cgtagtttgc gccgccgcgc gacacggcac aagggtcggg accgggggcg acgctccgcc
     2881 cggccgcatc actcctcggc gtcgtcgggc gacgggaacc cggggaactt tggaacggct
     2941 tcagggactg gcactcctgg tgcgagctcg acgggctcgt ctacgacagc tcgaacagcg
     3001 ccgcctcgcc aacggcggaa cctactcccc cggccgcggt gtggccgggg agtacgcggc
     3061 ggaaggatgg tcgtggagcc agtcgagtcc tactaccgac gcgtgaaggc gagggatgtc
     3121 aggcgctact cgcaggtcga ggtggcccgc catagtgtca ggaccgggtc taaggcgtgt
     3181 gggaagacgc gcaatgagcg agatcgagat cgccgcggac ctgtttgaga acgagcacac
     3241 ctgggaccag gtggaccgac ttgccgccga gctcccggag cgcaagacga tgagcgggcg
     3301 cgctgcacgg gagacgctgc tcgcatcgct ggacgggcgc ttcccggtga cccgttcgta
     3361 gctgactggc acacaaactg gcacccgagc aaaccatggg ggcccccagg ttctccggga
     3421 gcctcatcgt ttccgcaggt cacgtacgct gctcctggag cggacgacgg gattcgaacc
     3481 cgcgacccc
//
LOCUS       DASPWO010000303         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_637730, whole genome shotgun sequence.
ACCESSION   DASPWO010000303 DASPWO010000000
VERSION     DASPWO010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637730"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1526
                     /locus_tag="VEO00_07010"
     CDS             147..1526
                     /locus_tag="VEO00_07010"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYU57781.1"
                     /translation="MANVPLTQVSTDPYTNTSSFHQTELEPDTFSYGSTTVGVHQTGR
                     FTDGGANNIGWDTTTNNGATWTHGFLPSTTVYSTPPGPWARISDPSVAYDAADDVWMI
                     AGLAIDASVTGKAVLVSRSLDGGLTWQAPVTVSQGGAGNFYDKEWITCDNWPSSPYYG
                     NCYVQWDDAFQGNLFKMSRSTNGGTTWTASTVPSNSVLGGQPVVQPGGRVVVPIAGSG
                     LDSYISNNGGQSYTGPYNIANITAVGSPGMRDGTGLPSAEVDNAGVVYVAWNDCRFRT
                     TCTADDPVFSTSTDGQNWSAVKRVPTVPTNSSVGFFLVSIGVDHTTGGATARIGVTAY
                     FMADNNCSPSTCKIHAGFVSSTNGGTTWGAPVKILGPLDERGLANAGGYFLGDYMSTS
                     FGSNGKAYPVLANSSGTNCQLGNITSCHENMVAPTNGLFALGGSLPAVAGPVFSAPGA
                     SILLRSSAF"
     gene            complement(1781..2191)
                     /locus_tag="VEO00_07015"
     CDS             complement(1781..2191)
                     /locus_tag="VEO00_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYU57782.1"
                     /translation="MSEQRLRWDGDLRFEGRDSGGHRIAIGGEPDGAGAKASDLLPLS
                     LAACAVHDVVVILRKQRQDLRGLEVRIDSHQGQHPPWPFEAIRLRMIVSGDVDPVKAR
                     RALDLSMSKYCAVFATLSPTVRIETEVEVPDHTG"
     gene            2300..>2678
                     /locus_tag="VEO00_07020"
     CDS             2300..>2678
                     /locus_tag="VEO00_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57783.1"
                     /translation="MSGNRHRTAATAFALLAGLGMASQAAPARAAFPGQNGVIAWSRL
                     FLTTDAEIYGIRPNGRGERQISHNTRNDFGPAWASDGRRIAYGSSTASDVDVYVLSTV
                     DWTETNVTNDPGNADVEPAWSPSG"
BASE COUNT          434 a          937 c          909 g          398 t
ORIGIN      
        1 cggcctgccc tgccgtagac tactgcgccg ctttcgttcg tcgaggaaag gagtagagcg
       61 tgggctctcg caagcaacgt ttcgttcggg gatccgccat tctcgccggc ggccttgccc
      121 tcgcgctgtg gcaggggggc acggcgttgg cgaacgtccc cctgacccag gtcagcacgg
      181 atccgtacac gaacacctcc tcgtttcacc agacggagct cgagccggac accttctcct
      241 atggctcgac gaccgtcggc gtccatcaga ccggccggtt caccgacggc ggggcgaaca
      301 acatcggctg ggacaccacc accaacaacg gggccacgtg gactcacggg ttccttccga
      361 gcaccaccgt ctactcgacc ccgccggggc cgtgggcgcg catcagcgac ccctccgtgg
      421 cctacgacgc ggccgacgac gtctggatga tcgccggtct cgccatcgac gcgtccgtga
      481 ccgggaaggc cgtcctggtc agccgatccc tcgacggcgg cctcacgtgg caggcgccgg
      541 tcaccgtgtc ccagggtggt gcggggaact tctacgacaa ggaatggatc acctgcgaca
      601 actggccgag cagcccctac tacggcaact gctacgtcca gtgggatgac gcgttccagg
      661 gcaacctgtt caagatgagc cggtccacga acggcggaac cacctggacg gcttcgaccg
      721 tgcccagcaa ctcggtcctc ggtgggcagc cggtggtcca gccgggcggc cgcgtggtcg
      781 ttcccatcgc gggcagcggg ctcgactcgt acatctccaa caacgggggc cagagctaca
      841 cgggccccta caacatcgcc aacatcaccg cggtcggctc tcccggcatg cgggacggga
      901 cgggcctccc atcggccgag gtggacaacg cgggcgtggt gtacgtggca tggaacgact
      961 gccggttccg gactacgtgc acggccgacg acccggtgtt cagcacctcg accgacgggc
     1021 agaactggtc cgcggtgaag cgggttccca ccgtcccgac gaacagcagc gtggggttct
     1081 tcctggtgag catcggcgtc gaccacacca cgggcggggc cacggctcgg atcggggtca
     1141 cggcctactt catggcggac aacaactgct ctccgagcac ctgcaagatc cacgccgggt
     1201 tcgtctcctc gacgaacggc gggacgacgt ggggagctcc cgtgaagatc cttggcccac
     1261 tcgacgagcg tggcctggcg aacgccggcg gctatttcct gggggactac atgtccacct
     1321 cgttcggatc gaacgggaag gcctaccccg tgctggcgaa ctcgagcggg acgaactgcc
     1381 agctcggaaa catcacctcg tgccacgaga acatggtggc cccgaccaac gggctcttcg
     1441 cgctgggcgg ctcgctgccg gccgtcgccg ggcccgtgtt ctccgcaccg ggcgcgagca
     1501 tcctgcttcg ttcgagcgct ttctgagaag gcgacgggag gggggcgggc caccgccccc
     1561 ctccctcacg gcacccggcc gcgttcaagc cctcgccgac gtgacctcgg ggtcgaacgg
     1621 gtcgttcggc agccagacgc tctgctgcac cgccggtccc ggattcgacc tgccgagcgg
     1681 cgtcggcacg ccgaacgggc tgggcggctt ctgaccgggg cgatgctcga aggcggatcc
     1741 cggccctccg tcgggggttt cggttcgccg ctcgggatct ctacccggtg tgatccggga
     1801 cctccacctc ggtctcgatc cgaacggtcg ggctgagggt cgcgaagacc gcgcagtact
     1861 tcgacatcga gaggtccagc gcgcgacgag ccttcacggg atcgacgtcg ccgctcacga
     1921 tcatccgcaa cctgatcgct tcgaacggcc acggcgggtg ctgcccctgg tgcgagtcga
     1981 tgcggacctc caggccgcgc aggtcctgac gctgtttccg caggatgacc accacgtcgt
     2041 gaaccgcgca cgcggcgagg gagaggggga ggagatccga ggccttcgcc cccgccccgt
     2101 cgggctctcc accgatcgcg atccggtggc ccccagagtc ccgcccctcg aaccggaggt
     2161 cgccgtccca tctcagccgc tgctcggaca tgggcggcaa gtgtaggcgc ggacaatgcg
     2221 agaatgcccg gggatccccg cggcggttcg aggcgggccg ccatcggcga gggagccgac
     2281 gactgagtag gaggagagga tgagcgggaa tcgccaccga acggccgcga cggccttcgc
     2341 gctcctggcc ggactgggca tggcaagcca ggctgcgccg gcccgcgccg cgttcccggg
     2401 ccagaacggc gtcatcgcgt ggtcgaggct gttcctcacg accgacgcgg agatctacgg
     2461 gatccggccg aacgggcggg gtgagcggca gatctcccat aacacccgca atgacttcgg
     2521 gccggcgtgg gcgtcggacg gccggcggat cgcctatggg tcgagcacgg ccagtgacgt
     2581 cgacgtctac gtcctgtcga cggtcgactg gaccgaaacg aacgtcacca acgatcccgg
     2641 gaacgccgac gtcgagcccg cctggtcgcc gagcggcg
//
LOCUS       DASPWO010000304         7409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_976901, whole genome shotgun sequence.
ACCESSION   DASPWO010000304 DASPWO010000000
VERSION     DASPWO010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976901"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VEO00_07025"
     CDS             complement(<1..1092)
                     /locus_tag="VEO00_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYU57784.1"
                     /translation="MTEGREDLEPEHDRWRDHYRRAGERDADFETLSGEPLEPLYTPE
                     DVKDLDYERDLGYPGHDPMTRGVYHSMYRGRLWTMRQFAGFGTPAETNARYKFLLQHG
                     QGGLSVAFDMPTLMGRDSDDPRSEGEVGRCGVATDSLADFETLFDGIPLGDITTSMTI
                     SGPATILFAFFLAAAEKQGVDWKTLDGTLQTDILKEYIAQKEWIFPPRPHLRLIGDLI
                     EFCAREVPKFHPISVSGYHIREAGSTAAQELAVTLADGFSYVELGRQRGLDVDHFSQG
                     LSFFFNAHIDFFEEIAKFRAARRIWARWMRSRYGATDEKALWLRFHTQTAGCSLTAQQ
                     PENNIVRTAIEALAGVLGGTQSLHTNALDE"
     gene            complement(1089..2042)
                     /gene="meaB"
                     /locus_tag="VEO00_07030"
     CDS             complement(1089..2042)
                     /gene="meaB"
                     /locus_tag="VEO00_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017590551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HYU57785.1"
                     /translation="MKAEQLVDGVRAGDRRAIARAISMVEDDAAGLPDLIRGLYPHTG
                     NGHTVGLTGSPGVGKSTLTEQLVRAARAADRSVAVLAIDPTSPFTGGALLGDRLRMQS
                     HATDPQVFIRSMATRGHLGGMALAAPEAIRILDAAGKDVIFVETVGVGQAEVQVAGAT
                     DTTLVVVSPGWGDAVQVAKAGILEIADVFVVNKADKEGAAEAVRDLEHMLKMGQQADW
                     TPPVVKASAATGEGTDDVWEAIQSHRKHLEASGDLERTRERRLLEEVEGMAGLRLRAR
                     VAGLLDGDRAADVVQGLRARRIDPYRAAAILMERITEEGAG"
     gene            complement(2127..2960)
                     /locus_tag="VEO00_07035"
     CDS             complement(2127..2960)
                     /locus_tag="VEO00_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57786.1"
                     /translation="MTKATADRIGTWSEEAGVRIAGRSVEGAGFRLDLEIARAGAPVA
                     VVSDDGATGRVECTIVAPAAAGDPALGLSARVADLDRAGSGLLKAVLEPPESVTISHA
                     LYLDDLDRQAFLAALWEVAKVAETVRLIAGVAPAAPADVAAAEPEPAVTAPAGPEPAA
                     VAPAERPAVAMGPVEQPAPVMPAQPAAQPAQPAAAPIVPGYASAAAPATAAAPPWVAT
                     HVVPATGIATYLAPDPNAVGPTLQPGVELQVLERLGDWARVMGSNGWTGWVDARWLAP
                     R"
     gene            complement(3022..3438)
                     /locus_tag="VEO00_07040"
     CDS             complement(3022..3438)
                     /locus_tag="VEO00_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57787.1"
                     /translation="MPGAVEYLDEEPAGLAMMVGGLIEGNLAAHPERESLLRDAVIGI
                     IADDAGVAITLRIHPGLVTVANGLTGGSPGLLIRTTSEDLIALTAVPLRFGLPDALTG
                     DGRAVVRKLLAGKLKVRGMVRHLRTLTRLQRLLSVA"
     gene            complement(3448..4488)
                     /locus_tag="VEO00_07045"
     CDS             complement(3448..4488)
                     /locus_tag="VEO00_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYU57788.1"
                     /translation="MKAVVFEDVGVVRVDEVPEPEIEEPTDAVIRVSTAAICGSDLHM
                     LHGKTPMMPGDQIGHEGIGVIERAGQGVTRFAPGDRVVISFDIVCGECWFCRHGQSGL
                     CENFRNLGAGPFGGNLGGTQAERVRVPSADVNLLRVPEGLADEQAVFVGDILTTGVYA
                     AGIAGIAPGDTVAVIGAGPVGFFTAQAARLHDPADVLVLDLQPDRLALAEKVGATPLN
                     VMERNAQSAIYEMTDGRGADVAIDAVGHVSAWESALDVVRRGGRICVVGQYTTETVEF
                     QIGLVYTRGLTIVMSGLCPVRAWWERAMQAVVDGTIDPLPIISHTLPLAEAVEGYRLF
                     DAREATKVVLRP"
     gene            complement(4496..4915)
                     /locus_tag="VEO00_07050"
     CDS             complement(4496..4915)
                     /locus_tag="VEO00_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HYU57789.1"
                     /translation="MAEFPSEEWLKEYVERINASAEYREASATWEGDVSFVFEAEPDK
                     GVPEDVWAWLDLWHGDCREGKLVGPEEGEKAKFIIRAPYSRWKEVIKRELDPIKGMMQ
                     GKLKLKGDLPTIVRYVKASNELVNLAGTVPTEFVDEK"
     gene            complement(5074..5976)
                     /locus_tag="VEO00_07055"
     CDS             complement(5074..5976)
                     /locus_tag="VEO00_07055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HYU57790.1"
                     /translation="MARPGTARRAAAAGCRTRAGGRPGGEPGLSAQLPPPLGAPRPPP
                     PPPPGPLPLPGPQLPPPPGEGSPAGGSALPRATWRAVEAIPVFVIALVATTAIAAIAA
                     AFVNDRGLLNVVAQLGFEVTVPASVILWIRGVHRTSLRTVGLGRRPLGDLGFGTVSGL
                     VTYLGAFFAVAPVVVLLWEAFSGHEPTAPEQIPTDLSGGALVLLSVVVLVGAPVAEEL
                     FFRGFLFRALRARRSFVVSAVVSGAAFGLVHYLNGNWILVPIMFFVGIALAWIYERRG
                     SILAPMAAHFTFNVIGLTVILATR"
     gene            complement(5889..>7409)
                     /locus_tag="VEO00_07060"
     CDS             complement(5889..>7409)
                     /locus_tag="VEO00_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57791.1"
                     /translation="GDTLRIDTEAVYAREGRAAPERAAALADAGRLVRSIVEASPAEE
                     ALVLVVTAVPSSDMRRVGDVVTPVIAARGAPADLFPPAGMPHGLVSDSTRLDGIVTGD
                     DLAPTVLGFFDLPVPAEMTGSPIRATAGDAPFALHRRHLEHRRARLPVALGFGLPGLA
                     IVLAAFLRLLVRRPVPPAFDSALAFLLMAAAAIPAALLVAGYLPHLTYAWAVPAGLAA
                     CLGFGAAGWAARGRDPAMAVVVVAAAVAALVVVDTLLGGRAQTVSFGGVSAFEGRSYG
                     LRNFYVGPLVAGVVLAGSRFGPRRALLLVLGAAALAGLPGVGSEVGGCATLLVVAPLL
                     SFRLAGRPIRARALLWSLASLVVGLGLLFAADALLASAPGHPGRLAERVGSTGLGAFV
                     GVARERLETTVATIGRVPPAWLLVGWYALEAFAATRIRGRVGAGFDRVRGHRAAVWAL
                     ALAGLLAVAFNDTGISTAIGLAGPTFAAWLVPALLAAPPPPDAAPAPAAALAGSRA"
BASE COUNT         1064 a         2848 c         2598 g          899 t
ORIGIN      
        1 ctcgtcgagc gcgttcgtgt gcagcgactg cgtcccgccg agcacgccgg ccagcgcctc
       61 gatcgccgtg cgcacgatgt tgttctccgg ctgctgtgcc gtcagcgagc acccggcggt
      121 ctgggtgtgg aaccgcagcc acagcgcctt ctcgtcggtc gccccgtacc gcgaacgcat
      181 ccagcgggcc cagatccgcc gggcggcgcg aaacttcgcg atctcctcga agaagtcgat
      241 gtgcgcgttg aagaagaacg acaggccctg ggagaaatgg tcgacgtcaa ggccgcgctg
      301 ccgcccgagc tccacgtacg agaacccgtc ggccaacgtg acggccagct cctgcgcggc
      361 ggtcgagccg gcctcgcgga tgtggtagcc ggagacgctg atcggatgga acttcgggac
      421 ctcacgcgcg cagaactcga tcaagtcgcc gatcaggcgc aggtgcggcc gcggcgggaa
      481 gatccactcc ttctgcgcga tgtactcctt caggatgtcg gtctgcagcg tgccgtcgag
      541 cgtcttccag tccacgccct gcttctccgc ggcggcaagg aagaacgcga acaggatcgt
      601 cgccggcccg gagatggtca tcgacgtcgt gatgtcgccg aggggtatgc cgtcgaacag
      661 cgtctcgaag tcggccagcg agtcggtggc cacgccacag cggccaacct cgccctcgct
      721 tcgcgggtcg tcggagtcgc ggcccatcag ggtcggcatg tcgaaagcca ccgacagccc
      781 gccctggccg tgctgcagca ggaacttgta gcgcgcgttc gtctcggccg gcgttccgaa
      841 gccggcgaac tggcgcatcg tccacagccg cccgcggtac atcgagtggt acacgccgcg
      901 ggtcatcgga tcgtggcccg ggtagccgag gtcgcgctcg tagtcgaggt ccttcacgtc
      961 ctccggcgtg tagagcggct cgagcggctc gccggagagc gtctcgaagt cggcgtcgcg
     1021 ttcgccggcc cgccgatagt ggtcgcgcca gcggtcgtgc tccggctcga gatcctcgcg
     1081 tccctcggtc acccggcccc ctcctccgtg atccgctcca tcaggatagc cgccgcccgg
     1141 tacgggtcga tccggcgcgc gcgcagaccc tgcaccacgt cggccgcgcg gtcgccgtcc
     1201 aggagcccgg ccacccgggc gcgcagccgc aggccggcca tgccctcgac ctcttcgagc
     1261 aggcgccgct cgcgcgttcg ctcgaggtcc ccgctcgcct cgaggtgctt gcgatgggac
     1321 tggatcgcct cccacacgtc gtcggtcccc tctccggtgg cggccgacgc cttgaccacc
     1381 ggcggcgtcc agtccgcctg ctggcccatc ttcagcatgt gctccaggtc gcggacggcc
     1441 tcggcggcgc cctccttgtc ggccttgttc acgacgaaga cgtcggcgat ctccaggatg
     1501 cccgccttcg ccacctgcac ggcgtcgccc catccgggcg acacgaccac cagcgtggtg
     1561 tcggtcgctc cggccacctg aacctcggcc tgtccgacgc cgaccgtctc cacgaagatc
     1621 acgtccttcc cggccgcgtc gaggatgcgg atcgcctccg gcgccgcgag cgccatgccg
     1681 ccgaggtgcc ctcgggtggc catcgagcgg atgaacacct gcggatcggt ggcgtgggac
     1741 tgcatgcgca gccggtcgcc gagcagcgcg cccccggtga acggcgacgt ggggtcgatc
     1801 gccagcaccg ccaccgagcg gtcggcggca cgggccgccc ggacgagctg ctccgtcagg
     1861 gtggacttcc cgacccccgg cgagcccgtc agcccgaccg tgtgcccgtt cccggtgtgc
     1921 gggtagaggc cgcggatcag atccggcagg cccgccgcgt cgtcctcgac catggagatc
     1981 gcccgggcga tcgcccgccg gtcgccggcc cgcacgccat ccaccagctg ctcggccttc
     2041 acggaggaag cgtactccgg cgcccggcga cccccggggg ccgggcggcc gctccgggcg
     2101 cgaccggggt cagcgggcag gccgggtcag cgcggggcga gccagcgcgc gtccacccac
     2161 cccgtccagc cgttcgagcc catcacccgg gcccagtcgc ccaggcgctc cagcacctgg
     2221 agctcaacgc cgggctgcag ggtcggcccc accgcgttcg ggtccggcgc caggtacgtg
     2281 gcgatcccgg tggccggcac cacgtgggtc gccacccacg gaggggcggc cgccgtcgcg
     2341 ggggcagccg ccgacgcgta gcccggcacg atgggcgccg cggcgggctg cgccggctgc
     2401 gccgccggct gcgcgggcat gaccggcgcg ggctgctcga ccggccccat cgcgaccgcg
     2461 ggccgctcgg ccggggcaac ggcggcgggc tcgggcccgg ccggagccgt gacggccggc
     2521 tcgggttcgg cggcggccac gtccgccggg gccgccgggg cgacgccggc gatcaggcga
     2581 acggtctccg ccaccttcgc cacctcccag agggctgcca ggaacgcctg ccggtcgagg
     2641 tcgtcgaggt acagcgcgtg cgagatggtc acgctctcgg gcggttcgag cacggccttc
     2701 agcaggcccg agcccgcccg gtcgaggtcg gcgacccgtg ccgacaggcc gagcgccggg
     2761 tcgccggctg cggccggcgc cacgatcgtg cactccaccc tcccggtcgc cccgtcgtcc
     2821 gatacgacag ccaccggcgc gccggcccgg gcgatctcca ggtcgaggcg gaacccagcc
     2881 ccctccacgg agcgcccggc aatccggacg ccagcctcct cgctccaggt gccgatccgg
     2941 tccgccgtcg ccttggtcat ctccgtccct ccgttcgcca cgtcatcggc acgttcaccg
     3001 taccccttca ggccacaccc ttcaggccac ggacaggagg cgctgcagtc gcgtcagggt
     3061 gcggagatgg cggaccatcc cgcgaacctt cagcttcccg gccagcagct tgcgcacgac
     3121 ggcccggccg tcaccggtga gcgcgtcggg gagcccgaag cgcagcggca ccgcggtgag
     3181 ggcgatcagg tcctcggacg tcgtccggat caggagcccg gggctgccgc cggtcagccc
     3241 gttcgccacg gtcaccaggc cgggatggat gcgcagcgtg atcgccacgc cggcgtcgtc
     3301 ggcgatgatc ccgatcacgg cgtcgcgcag caatgactcg cgctcggggt gggcggcgag
     3361 gttcccctcg atgagcccgc cgaccatcat cgccagcccg gcgggttcct cgtccaggta
     3421 ctcgaccgcg cccggcatcg gatccgacta cggcctgagg acgaccttcg tcgcctcgcg
     3481 cgcgtcgaac aggcggtagc cctccacggc ctcggcgagc ggcagggtgt gactgatgat
     3541 cggcagcggg tcgatcgtgc cgtccaccac ggcctgcatg gcgcgctccc accaggcgcg
     3601 gaccgggcag agccccgaca tcacgatcgt cagcccgcgc gtgtagacga gcccgatctg
     3661 gaactcgact gtctcggtcg tgtactggcc gaccacgcag atccgtccgc cgcggcggac
     3721 cacgtcgagc gccgactccc aggccgagac gtggcccacc gcgtcgatcg cgacgtcggc
     3781 gccgcggccg tccgtcatct cgtagatcgc gctctgcgcg ttgcgctcca tgacgttcag
     3841 cggcgtcgcg cccaccttct cggccagcgc caggcggtcc ggctgcaggt cgagcaccag
     3901 cacgtcggcc ggatcgtgca gccgggccgc ctgggccgtg aagaacccga ccggtcccgc
     3961 cccgatcacc gcgacggtgt ctcccggggc gatgccggcg ataccggccg cgtacacgcc
     4021 ggtggtcagg atgtcgccga cgaacaccgc ctgctcgtcg gccagcccct ccggcacacg
     4081 aagcaggttg acgtcggcgc tcgggacgcg cacccgctcg gcctgggtcc cgcccaaatt
     4141 cccgccgaac ggcccggccc cgaggttccg gaagttctcg cacagcccgc tctgcccgtg
     4201 gcggcagaac cagcactcgc cgcagacgat gtcgaacgag atcaccacgc gatcaccggg
     4261 ggcgaagcgg gtcacgccct ggccggcgcg ctcgatgacg ccgatcccct cgtggccgat
     4321 ctgatcgccc ggcatcatcg gcgtcttgcc gtggagcatg tgcaggtccg aaccgcagat
     4381 cgcggcggtg ctgacccgga tgacggcgtc ggtcggttcc tcgatctcgg gctcggggac
     4441 ctcgtcgacg cgaacgaccc cgacgtcttc gaagacgacg gccttcaccg aggggctact
     4501 tctcgtcgac gaactcggtc gggaccgtgc ccgccaggtt caccagctcg ttcgacgcct
     4561 tcacgtagcg cacgatcgtc ggcaggtcgc ctttcagctt gagcttgccc tgcatcatcc
     4621 ccttgatcgg gtcgagctcc ctcttgatca cttccttcca ccgcgagtac ggcgcgcgga
     4681 tgatgaactt cgccttctcg ccctcctccg ggccgacgag cttcccctcg cggcagtcgc
     4741 cgtgccacag gtcgagccag gcccagacgt cttccgggac gcccttgtcg ggctcggcct
     4801 cgaagacgaa cgagacgtcg ccctcccagg tggccgacgc ctcccggtac tcggcggacg
     4861 cgttgatccg ctcgacgtac tccttcagcc attcctcgct cgggaactcg gccacggcga
     4921 cctcctcggt ccggggtggc gggggacgag ccgcggtggg ctcgcccgcc gccgggatgc
     4981 gccgccggaa gtaaagcagg tccccgcggg gcccgcgagc cgccggggag ctgagcacag
     5041 gaccacccac ggcgagggga cccccacggc ggatcagcgg gtggcgagga tgacggtcag
     5101 cccgatcacg ttgaacgtga agtgggcggc catcggcgcc aggatgctgc cgcgccgctc
     5161 gtagatccag gccagagcga tgccgacgaa gaacatgatc gggacgagga tccagttgcc
     5221 gttcaggtag tggaccaggc cgaacgcggc tcccgagacc accgccgaca ccacgaagct
     5281 gcgacgggcg cgcagcgccc ggaacaggaa cccgcggaag aacagctcct cggccaccgg
     5341 ggcgccgacc aggaccacca cggagagcag cacgagcgcg ccgccggaca ggtccgtcgg
     5401 gatctgctcc ggagccgtgg gctcgtgtcc ggagaaggcc tcccacagga gcacgaccac
     5461 cggggccacc gcgaagaacg cccccaggta ggtgaccagg cccgacaccg tgccgaaacc
     5521 caggtcgccg agcggccgcc ggccgaggcc caccgtccgc agcgaggtgc ggtgtacgcc
     5581 gcggatccag aggatcacgc ttgccgggac cgtcacctcg aaccccagct gcgccaccac
     5641 gttcagcagg ccccgatcgt tcacgaaagc cgccgcgatc gcagcgatgg ccgtcgtcgc
     5701 caccagggcg atgacgaaga ccgggatcgc ctcgacggcg cgccaggtgg cccgcgggag
     5761 cgcggagccc cccgccggcg agccctctcc cgggggcggc gggagctgcg ggcccgggag
     5821 cggcagcgga cctggcggcg gcgggggcgg cggtctgggc gcgccgagcg gcggcggcag
     5881 ctgcgcgctc aggcccggct ccccgccagg gcggccgccg gcgcgggtgc ggcatccggc
     5941 ggcggcggcg cggcgagcag tgccgggacg agccaagccg cgaaggtcgg tcccgccagc
     6001 ccgatggccg tcgagatgcc ggtatcgttg aacgcgaccg ccaggaggcc ggccagggcg
     6061 agcgcccaga cggccgccct gtgccctcgg acccggtcga acccggcccc cacccggccg
     6121 cggatgcgcg tggcggcgaa ggcctccagc gcgtaccacc cgaccaggag ccaggcgggt
     6181 ggcacccggc cgatcgtcgc gaccgtcgtc tccaggcgct ctcgggcgac tcccacgaag
     6241 gcgcccaggc cggtgctccc gacccgctcg gccagccgcc cggggtggcc gggggccgag
     6301 gccagcagcg cgtcggccgc gaacagcagc ccgagtccga cgacgagcga cgccagggac
     6361 cacagcagcg cccgcgcccg gatcgggcgc ccggcaaggc ggaacgacag gagcggcgcc
     6421 accaccagca gggtggcaca tccgccgacc tccgacccga ccccgggcag ccctgccagc
     6481 gcggccgctc ccaggacgag aagcagggcc ctccgcggcc cgaaccgcga tccggccaac
     6541 acaaccccgg cgacgagcgg cccgacgtag aagttccgca gcccgtagga gcgcccctcg
     6601 aaggccgaca ccccgccgaa cgagaccgtc tgggcccgcc cgccgagcag ggtatccaca
     6661 acgaccagcg cggccaccgc cgccgccacg accaccaccg ccatcgccgg gtcgcgccct
     6721 cgggcggccc agcccgcggc cccgaacccg agacacgccg ccaggcccgc cggcacggcc
     6781 caggcgtacg tgaggtgcgg aagatagccg gcgacgagca gtgccgccgg gatcgccgcc
     6841 gccgccatca gcaggaacgc cagcgccgaa tcgaacgccg ggggcacggg ccgccgcact
     6901 aggagccgca ggaacgccgc gagcacgatc gccaggccgg gcaggccgaa gccgagcgcc
     6961 accggaaggc gcgcgcgacg gtgctcgagg tgccggcgat gcagggcgaa cggcgcgtcc
     7021 cccgccgtgg cgcggatcgg cgagccggtc atctccgccg gaaccggcag gtcgaagaac
     7081 ccgaggacgg tcggcgccag gtcgtcgccc gtgacgatgc cgtcgaggcg cgtggagtcc
     7141 gagaccagcc cgtgcggcat ccccgccggg gggaacagat cggccggcgc gccgcgcgcg
     7201 gcgatcacgg gcgtgaccac gtcgccgacc cggcgcatgt ccgaggacgg caccgccgtg
     7261 acgaccagca ccagcgcctc ctccgccggc gaggcctcga cgatcgagcg gaccagccgg
     7321 cccgcgtcgg ccagcgcagc ggcccgttcc ggcgccgcgc ggccctcccg cgcgtacacc
     7381 gcctcggtgt cgatccgcaa cgtgtcgcc
//
LOCUS       DASPWO010000305         3943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_127747, whole genome shotgun sequence.
ACCESSION   DASPWO010000305 DASPWO010000000
VERSION     DASPWO010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127747"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VEO00_07065"
     CDS             complement(<1..269)
                     /locus_tag="VEO00_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57792.1"
                     /translation="MIQAARDRILSVVEPMAPLELPLQEAWGCVLAGDVAAEFDVPEF
                     SAALVDGFAVRASDVFAASEAAPAALSIVGRSLVGQHPESTVGWG"
     gene            complement(274..1152)
                     /gene="galU"
                     /locus_tag="VEO00_07070"
     CDS             complement(274..1152)
                     /gene="galU"
                     /locus_tag="VEO00_07070"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HYU57793.1"
                     /translation="MRLKKAVIPAAGLGTRFLPATKAQPKEMLPLVDKPAIQYVVEEA
                     VRAGLSDILMITGRGKRTLEDHFDRSYELENWLETRGKHDLLKQVREISEMAAIHYIR
                     QRDPSGLGAAVAVAEPHVDGDPFAVLLGDDIIDVGEPLLERMIHVYERYGRSVIAVQE
                     VPKDQIHLYGAVSPEFVEDGLARIETIVEKPDPAEAPSNLAAIGRYLLTPEIFDALRA
                     TTPDRGGEVQLTDAINQLAQEQAVYAYVYEGARYDIGKKLDYLRATVELAVRREDLGP
                     DFKAFLSDFVQRKKLV"
     gene            complement(1226..3169)
                     /locus_tag="VEO00_07075"
     CDS             complement(1226..3169)
                     /locus_tag="VEO00_07075"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYU57794.1"
                     /translation="MRSPLSLRARLTLFFIGIVVVPLVVAGLVVERLAVRQADARTDS
                     TLDTAVPGLRTAVLDRQDRAATVTGLVAAQAVADAEGGLGLDERRTEAGLDYLLVVRS
                     GEVLAVSMRPKGFLPGVPVRAQDLAGPNPPAGLVAGARVEIEARAGIEVRGGFFLDRP
                     FLEALPVLAASVSRGEVVASPVQPPPRVNLEATGPFDAGPNLRGLFVPFENEPGSGLV
                     ALVRKPSGGALSGASGAILLLLGVGVVIASGLGYGLARLIARPLQRLAEGALAVAGGD
                     LDQSVPATGDDDVARLGAAFNTMTANLRDSIGELTHSRDELRHGLDRLGATLSASHDV
                     DGLLAVVLETAAGTLGAEAGAAYLRLPRRQEIASRAVVGVRGPIRLRVGEGLAGYAAA
                     EAQALRVGPEGRLAPEEPAAETALAVPLSRGEEIYGVLALYGRAGGRAFSDDDLVTLA
                     SLAAQAAVAIENVHLQREAERLAVTDALTGVWNRRYLQETMGHELDRAGRYGRTVSLL
                     LLDLDHFKEVNDRYGHVRGDLVLVELSRRIQDSIRANIDVLARYGGEEFVILLPETGL
                     QGGLAAAEKVRAVVHDAPFGANGDAIALTVSVGVATYPADGRDADDLLRAADRALYRA
                     KRAGRDRVMAAARDEQQPLTEQP"
     gene            3227..3829
                     /locus_tag="VEO00_07080"
     CDS             3227..3829
                     /locus_tag="VEO00_07080"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HYU57795.1"
                     /translation="MTDSGDPNSPTGLAGRKEALRRAMREARSSLPPPDRAERAARVE
                     ARLLAIPEVVAARCVLLFYAFGSEIPTRVLAARLRTGGRRVLLPYLSGGAMEAAEVGP
                     EDPLVVSGYGPKEPARRVPVDPAEVDLVLTPGLAFDRRGGRLGYGGGHYDRYLARLGG
                     HATRIGIGFEEQVVDEVPAGPEDERVDLVATDRETIDARA"
BASE COUNT          529 a         1469 c         1429 g          516 t
ORIGIN      
        1 tcgccccagc cgacggtgga ctccggatgc tgcccgacca gcgaccggcc gacgatcgac
       61 agcgccgccg gcgccgcctc ggaggcggcg aacacgtccg acgcgcgcac cgcgaacccg
      121 tcgacgaggg ctgccgagaa ctccggcacg tcgaactcgg cggccacgtc gcctgccagg
      181 acgcaacccc acgcctcctg gagcggcagc tcgaggggcg ccatcggctc gaccaccgag
      241 aggatgcggt cgcgcgcggc ctggatcacg agctcagacg agcttcttcc gctgcacgaa
      301 gtccgagagg aacgccttga agtccgggcc gaggtcctcg cgccggacgg ccagctcgac
      361 cgtggcccgc aagtagtcga gcttcttccc gatgtcgtag cgcgcgccct cgtacacgta
      421 cgcgtagacg gcctgctcct gggccagctg gttgatcgcg tcggtcagct gcacctcgcc
      481 gccgcggtcc ggcgtggtcg cgcgcagcgc gtcgaagatc tccggcgtca gcaggtagcg
      541 gccgatcgcg gcgaggttcg acggcgcctc cgccgggtcg ggcttctcga cgatcgtctc
      601 gatccgcgcc aggccgtctt cgacgaactc cggactgacg gccccgtaca ggtggatctg
      661 gtccttcggg acctcctgaa cggcgatcac gctccgcccg tagcgctcgt agacgtggat
      721 catccgctcg aggagcggct cgccgacgtc gatgatgtcg tcgccgagca gcaccgcgaa
      781 cgggtcgccg tccacgtgcg gctccgccac cgccaccgcg gccccgaggc ccgacgggtc
      841 gcgctggcgg atgtagtgga tcgcggccat ctcggagatc tcgcggacct gcttcagcag
      901 gtcgtgcttg ccgcgcgtct ccagccagtt ctccagctcg taggagcggt cgaagtggtc
      961 ctcgagcgtt cgtttcccgc ggccggtgat catcaggatg tcggacaggc cggcgcgcac
     1021 cgcctcctcg accacgtact ggatcgccgg cttgtcgacg agggggagca tctccttcgg
     1081 ctgggccttc gtcgccggca ggaaccgcgt cccgagcccg gccgccggga tgaccgcctt
     1141 cttcaaccgc atgaccgctc cctccccgcg gaagtgccaa gtctatgcgt ggcggcggtg
     1201 ccaccgtgag cgattcagga gggatctacg gctgttcggt caatggttgt tgctcgtcgc
     1261 gggcggcggc catcacccgg tcgcgaccgg cgcgcttggc ccggtacagg gcacggtccg
     1321 ccgcgcgcag caggtcgtcc gcgtcgcggc cgtcggcggg gtaggtcgcc acgccgacgc
     1381 tgacggtcag cgcgatggcg tccccgttcg cgccgaacgg cgcgtcgtgc acgaccgccc
     1441 ggaccttctc cgctgccgcc agcccgccct gcaggccggt ctccggcagc aggatcacga
     1501 actcctcgcc gccgtagcgg gccagcacgt cgatgttcgc gcggatcgag tcctggatcc
     1561 ggcggctcag ctcgacgagc acgaggtcgc cgcgcacgtg cccgtagcga tcgttgacct
     1621 ccttgaagtg gtcgaggtcg aggagcagga gggagaccgt gcgcccgtag cgcccggccc
     1681 ggtcgagctc gtgccccatc gtctcctgca ggtagcggcg gttccagacg ccggtcagcg
     1741 cgtcggtcac cgccaggcgc tcggcctccc gctggagatg cacgttctcg atggccacgg
     1801 cggcctgcgc ggcgagggac gcgagcgtga ccaggtcgtc gtcgctgaac gcccggcccc
     1861 ctgcccgccc gtacagcgcg agcacgccgt agatctcctc cccgcgtgag agcggaacgg
     1921 ccagcgcggt ctccgccgcc ggctcctcgg gggccagccg tccttccgga ccgacccgca
     1981 gggcctgcgc ctccgccgcc gcgtagccgg cgagcccctc gccgacgcgc agccggatcg
     2041 gaccccggac cccgaccacc gcccgcgagg cgatctcctg gcgtcgcggc aggcgcaggt
     2101 acgccgcgcc ggcttccgcc cccagcgtcc ccgccgcggt ctcgaggacg accgcgagca
     2161 ggccgtcgac gtcgtgcgag gcggagagcg tcgcgccgag gcggtcaagc ccgtgtcgca
     2221 gctcgtcgcg cgagtgggtc agctctccga tcgagtcgcg caggttcgcg gtcatggtgt
     2281 tgaacgccgc gccgaggcgc gccacgtcgt cgtcgccggt ggccggcacg ctctggtcga
     2341 ggtccccgcc ggcgacggcc agggcgccct cggccagccg ctgcagcggc cgggcgatca
     2401 ggcgggccag gccgtacccc agaccgctgg cgatcaccac gcccaccccg agcagcaaca
     2461 ggatcgcgcc gctcgcccca gacagggccc cgccgctcgg cttgcgcacc agggcaacca
     2521 gccccgatcc gggttcgttc tcgaagggga cgaacagtcc gcgcaggttc ggcccggcgt
     2581 cgaacgggcc cgtcgcctcg aggttcacgc gcggcggcgg ctggaccggc gaggcgacca
     2641 cctcgccgcg ggacaccgat gccgcgagca ccgggagcgc ctccaggaac ggacggtcga
     2701 ggaagaaccc cccgcggacc tcgatgccgg cacgggcctc gatctcgacc cgggcgccgg
     2761 ccacgaggcc ggccgggggg ttcggcccgg ccaggtcctg cgcccgcacc ggcacgccgg
     2821 ggaggaaccc cttcgggcgc atcgagacgg ccaggacctc tcccgagcgc accaccagca
     2881 ggtagtcgag cccggcctcg gtgcggcgct cgtcgagccc caggccgccc tcggcgtcgg
     2941 cgacggcctg agcggcgacc agcccggtga ccgtggccgc ccggtcctgc cggtcgagga
     3001 cggccgtgcg cagaccgggc acggcggtgt cgagcgtgct atcggtacgg gcgtcggcct
     3061 ggcgaaccgc caggcgctcc acgaccagcc cggcgacgac cagggggacg accacgatgc
     3121 cgatgaagaa gagggtgagg cgggcgcgaa gggagagagg cgagcgcacg atcagatccg
     3181 gggtcggagt ctactcccac aggtttcggc agatcgggcg cgagacttga ccgattcggg
     3241 cgatccgaac agcccgaccg gcctcgccgg gcgcaaggag gccctgcgcc gcgccatgcg
     3301 ggaggcgcgc tcctccctcc ccccgccgga ccgggccgag cgggccgccc gggtggaggc
     3361 ccgcctcctg gcgatcccgg aggtcgtggc ggcccggtgc gtcctcctct tctatgcgtt
     3421 cggcagcgag attccgacgc gggtgctggc cgcgcgcctg cgaacgggcg gtcgccgggt
     3481 gctgctcccc tacctgagcg gaggggcgat ggaggccgcc gaagtcggcc ccgaggaccc
     3541 cctggtcgtc tcgggctacg ggccgaagga gccggcccgg cgcgtcccgg tcgacccggc
     3601 cgaggtcgac ctcgtcctca cgccgggcct ggccttcgac cgtcgcggcg ggcggctcgg
     3661 gtatggcggg ggccactacg accgctacct ggccaggctt gggggccacg ccacccggat
     3721 cggcatcggg ttcgaggagc aggtggtgga cgaggtcccg gcgggcccgg aggacgaacg
     3781 ggtcgacctg gtcgccaccg accgcgagac gatcgacgcg cgggcctgac ggggtccggc
     3841 ccagggtcgc gtcgaggcag cgtctcgggg tggcttctgg gggctccgct acaatcgccg
     3901 cgatgcccac ctatgagtac gtctgcgaga acggccaccg ggt
//
LOCUS       DASPWO010000306         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1066999, whole genome shotgun sequence.
ACCESSION   DASPWO010000306 DASPWO010000000
VERSION     DASPWO010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066999"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1115
                     /locus_tag="VEO00_07085"
     CDS             129..1115
                     /locus_tag="VEO00_07085"
                     /inference="COORDINATES: protein motif:HMM:NF023726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3631 domain-containing protein"
                     /protein_id="HYU57796.1"
                     /translation="MLELIVPDPLRTASVSDAALFRTIAAAERPPVILLDEVDAVFRP
                     KSDREDLRALLNAGYRLGADVARCELVGRKVEVRRYSAFGPKVLAGIGSLPDTIADRS
                     IPIRLRRRARGEAVARFRFREARAETEDLRDALAAWAAWAVEQLVEARPSVPPELNDR
                     AADGWEPLLAIADAAGGEWPTAARAAAVELHSAEDEGDETFGVQLLADVARVFEATGS
                     DRISTAELVEALKEHGEGPWAEWWSRSEDLRGPGHRIARLLRPYGISSKKIRAGDRTA
                     QGYEAGQFADAWARYLPVPVFREKGTEHRNTAGRRPYSTGTGSQPRGVEKGL"
     gene            1743..2198
                     /locus_tag="VEO00_07090"
     CDS             1743..2198
                     /locus_tag="VEO00_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57797.1"
                     /translation="MPTPSKWSAARRRAILEILAVGGSRRTAAAAAGIDHATLLRWLD
                     RGATSKGDTTWRRFAEDVAKAEASPRVRALSIVHEAMPDSPMLAWRFLERREEGYAPT
                     PPAPPPPQGPLVIELSLNPGPVPPPVELPASSVIEVLPPHDPTDPEDAS"
     gene            2553..>2834
                     /locus_tag="VEO00_07095"
     CDS             2553..>2834
                     /locus_tag="VEO00_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57798.1"
                     /translation="MGELLAVTQNGSLPEQAAAAWGYYTMSAEGDAEDARKVADAATS
                     DQLESELLLKFCRARTANMVATERFRTLARRVAAALLALGTLGGEHATEL"
BASE COUNT          444 a          973 c         1012 g          405 t
ORIGIN      
        1 cccggtccca ggtcgtcgcc gtcgcgctgt atgtggcgca ttcgcacgcg attgccgccg
       61 cggaagtgac cccttacctc gcggtaacgt cgccggaaaa gcggtccggg aagacgcgcc
      121 tactagaagt gctcgagctc attgtccccg acccgctgcg gacggcgagc gtgtcggacg
      181 ccgcgctgtt ccgcacgatc gcggctgccg agcggcctcc ggtgatcctg ctggatgagg
      241 tggacgcggt gttccggccg aagtcggacc gcgaggacct ccgggcgctc ctcaacgccg
      301 ggtaccggct cggtgccgac gtggcccgtt gcgagctggt cggccgcaag gtcgaggttc
      361 gccgctactc ggcgttcggc ccgaaggtcc ttgccgggat cggctcgcta ccggacacga
      421 tcgctgatcg ctccatcccg atccggctcc ggcgccgggc ccggggtgag gcggtcgccc
      481 gattcaggtt ccgtgaggct cgcgccgaaa ccgaggacct acgcgacgcc ctggcggcat
      541 gggcggcatg ggcggtggag cagctcgtcg aggcccggcc aagcgtcccg ccggagctga
      601 acgaccgagc ggcggacggg tgggagccgc tgctcgccat cgccgacgcg gctggcgggg
      661 agtggccgac agcggcccgg gccgcggcgg tcgagctgca ttcggccgag gacgaaggcg
      721 acgagacgtt cggcgtccag ctcctcgccg acgttgcccg ggtgttcgag gccaccggca
      781 gcgaccgcat ctccacggcc gagctcgtcg aggcgctgaa ggaacacggc gaaggcccct
      841 gggccgagtg gtggtcccgg agcgaagacc tgcgcggacc aggccaccgc atcgcccgcc
      901 tgttgcggcc ctacggcatc agctcgaaga agatccgcgc cggggaccgg accgcgcagg
      961 gttacgaggc cggccagttc gccgatgcgt gggcccggta cctccccgtg ccggtgttcc
     1021 gtgaaaaagg aacggaacat cggaacaccg caggtagacg gccatattcg accggaacag
     1081 gctcccagcc ccgaggggtc gaaaagggcc tctgagctgg gacgttccgt tgttccgttc
     1141 ttttcgtcgg aacagaggaa cggggagaag aaggagcatg atgccggccc gccctggcaa
     1201 ccgtccgcgg aggcctgctg ggcagagcgc cgctacaagg gcgagccctc cggctactgc
     1261 tggcaccccg aggacgcgca cctcacgggc ggtgcctgca cggtcaatgg ctgcgactgc
     1321 acggtcaatg gctgcgactg cgccggctac gcgcccgagc gcgtcatgga gcgagccagc
     1381 cacctcctcc gccgggagct cggcgcgcag gaggtgaagc tgtgaggtcg tggacaacgt
     1441 catacgtcgc cgaatctcct tcggtcgccc agccaggtcc gtggtgcggt cctcggcctg
     1501 agcatccgcg tcctctcctg ccgcgtggcg agccactggt cgagttcctc gcgctcatgc
     1561 aaaaccgcag gtcagagggc atatgggggg tcggcagggg tcgagtcgtg aacgaacgcc
     1621 cccagcgcag aacggcgccc atgcacaatc tcggcgcctc tcgcgtaccg caccacttcc
     1681 ggtgcgattc ggctctggtg cgcttccgcc gaacgatatt cggcccggga tagggtgagc
     1741 tagtgccaac tccgtcgaag tggtcagcag cacggcgccg agccatcctg gagatcctcg
     1801 ctgtcggcgg cagccggcgc accgcggccg cggcggccgg gatcgaccac gccacgctgt
     1861 tgcgctggct cgaccggggc gccacgtcaa agggcgacac gacctggagg cgattcgccg
     1921 aggacgtggc gaaggccgag gccagcccgc gggtccgggc gctgtcgatc gttcacgagg
     1981 ccatgcccga ctcgccgatg ctcgcctggc ggttcttaga gcgccgcgaa gagggctacg
     2041 cgcccacgcc gcctgcccca ccgccaccgc aagggccgct cgtcatcgag ctctcgctga
     2101 atcccgggcc cgtgccgccg ccagttgagc tccccgcctc ctcggtcatc gaagtcctgc
     2161 cgccgcacga cccgaccgac ccggaggatg cgtcgtgatc gacgacgagc ggccgtgccg
     2221 gaccgccacg caggctcgct tcgcaacagc tgtgcacgaa gccggccacg tcgtcgcggc
     2281 gtatctgttc agccgggtcc tcgtggaggc cgtcgtgcag cccgagtcgg cgcacgaatt
     2341 cgggcacacg ttcggcgatg gcgagcaccc gggccacatg ggctactggc cccaggaggc
     2401 cctggacctg gagcaggccc cgaacctgga gcaggccatc ggggacatct gcatcagcta
     2461 tgccggggag gccaattcgc gcatcgcgct cgcgctcggg agcgtcgccg atggcgaggt
     2521 gggggtgcgg gccctcacgg cggacgacct cgatgggcga actgctggcg gtcacgcaaa
     2581 acgggtcgtt accggagcag gccgcagcgg cttggggcta ctacacgatg tcggcggaag
     2641 gcgatgccga ggacgcacgg aaagtggccg acgcggcgac gtcggatcaa ctcgagtccg
     2701 agctactgct caagttctgc cgcgccagga cggccaacat ggtcgccacc gagcgtttcc
     2761 ggactctggc tcggagagtg gccgcggcct tgctggcgct cgggacactg ggcggcgaac
     2821 acgcaacaga gttg
//
LOCUS       DASPWO010000307         4542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_894759, whole genome shotgun sequence.
ACCESSION   DASPWO010000307 DASPWO010000000
VERSION     DASPWO010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894759"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..287)
                     /locus_tag="VEO00_07100"
     CDS             complement(18..287)
                     /locus_tag="VEO00_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57799.1"
                     /translation="MGAVRAVSALRVEHRDQRGRARLRVGDCPYLPEELNVFCDWCRF
                     DFATMEGNSPCDDPLTCEHGVVPRAHVENYHRWAAAREALTTPGA"
     gene            507..824
                     /locus_tag="VEO00_07105"
     CDS             507..824
                     /locus_tag="VEO00_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57800.1"
                     /translation="MTTMHRTIQVDPTTDFDHAAVTVPAPREESEHDAEVELMHSIDQ
                     LVRTARHDPELKELILAWEDEPPQDPRPLLRHGEFTCSGCHMVLDRRRLADPASMRCA
                     DCA"
     gene            838..1257
                     /locus_tag="VEO00_07110"
     CDS             838..1257
                     /locus_tag="VEO00_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57801.1"
                     /translation="MTRRDRALIVSEDADLVRRWTAWLEQAEFDTAFCPGPLAAFECP
                     RANGDSCPRREWADVAVVDVGQLARLGLSGIGAARSCTTLPDDGRTVVAMEGGTHHRH
                     GRRRPTVTHPVEPDSLVGVLRATAHRHPSVALPPKAS"
     gene            complement(1293..1685)
                     /locus_tag="VEO00_07115"
     CDS             complement(1293..1685)
                     /locus_tag="VEO00_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYU57802.1"
                     /translation="MLGWVGDIERVTLDNETFRTVLFTGEHVQLTVMRLGPGEDIGRE
                     RHPHLDQFLRVERGKGRVELSSQEDRVEETHDVREDWAIIVPAGVWHNVVNTGDGELQ
                     LYSLYAPPQHPPGTVHVTRADADAAEHR"
     gene            complement(1723..2355)
                     /locus_tag="VEO00_07120"
     CDS             complement(1723..2355)
                     /locus_tag="VEO00_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003959661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYU57803.1"
                     /translation="MRDFTEERAHGHERWAHERYRHEARHHGGHHGRYRFGRGFGPWG
                     RGEFPGPFYGRGARAGRGDVRAAILALLAERPMHGYQVIQELTERTRGVWRPSPGSVY
                     PTLQMLEDEGLVTSSEADGRRVFELTDAGRQEVARRGGEAPWAMAADASPVVALWEVG
                     RGVAMASMQVAQTGDEQQVARAIEILREARKSLYRLLADDESTSDDHTEA"
     gene            2543..3199
                     /gene="msrA"
                     /locus_tag="VEO00_07125"
     CDS             2543..3199
                     /gene="msrA"
                     /locus_tag="VEO00_07125"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HYU57804.1"
                     /translation="MLFSRHKTTLPDAREALPGRDRPAFAIPERHEVLGTPILPPYPQ
                     GLQVATFALGCFWSAERLFWKIPGVFTTAVGYTGGYTPNPTYEEVCSGRTGHAEAVLV
                     AFDPATVSYRELVRVFFEEHDPTQGMRQGNDVGTQSRSAIYVADEEQRRVAEMARDAY
                     DADLRARGFDPVTTEIDVAGPFYYAEAYHQQYLHKVPNGYCGLEGTGVRCSIPEGSAS
                     "
     gene            3196..3615
                     /gene="msrB"
                     /locus_tag="VEO00_07130"
     CDS             3196..3615
                     /gene="msrB"
                     /locus_tag="VEO00_07130"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HYU57805.1"
                     /translation="MTDELRDTELPATEEAWRARLTPEQYRVLREAGTERAFTGAYVD
                     ADDDGIYRCAACGNPLFESRTKYHSGTGWPSFTAAVSPDAVELLEDTAYGMVRTEVRC
                     ARCHSHLGHVFPDGPPEAGGQRYCMNSVALDLETRNG"
     gene            complement(3631..>4542)
                     /locus_tag="VEO00_07135"
     CDS             complement(3631..>4542)
                     /locus_tag="VEO00_07135"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HYU57806.1"
                     /translation="EPKLLSEAELERLSRTFMRAIADLIGPDVDVPAPDVNTNPRIMG
                     WMVDEYARRYGPTPAVITGKPVVLGGSLGRDAATGRGAALCLDRLARARGWARAEAPL
                     AIQGYGNAGSWLARIAAGMGYPVVAASDSRGAIVSHRGLDPDAVLAHKEATGSVIGFA
                     DAETIPGEELPSVDCSVLALAALEEAVTQDNAVDVKAATVLEVANYPITPDGEAILLD
                     RGISVVPDTLASGGGVAVSYLEWVQNQQREAWSADRVNEVLATIMETATDQVLARAEA
                     DGLSLRQAAYLIGVGRVAEAEIARGFR"
BASE COUNT          607 a         1710 c         1600 g          625 t
ORIGIN      
        1 gagcccccgc cgtcgtctca ggcgcccggt gtcgtcagcg cctcgcgtgc ggcggcccag
       61 cgatggtagt tctccacgtg ggcgcggggg accacgccgt gctcgcaagt gagggggtcg
      121 tcgcacgggg aatttccctc catcgtggcg aagtcgaacc ggcaccagtc gcagaacacg
      181 ttcagctcct ccggcaggta ggggcaatcc cccacgcgca gccgcgctcg ccctcgttgg
      241 tcgcgatgtt ccacccgcag tgcggacact gctcgaactg ctcccatcgc gccatggtct
      301 cctccttccg tcctgaccgc accgtaggag cgggcgggac gggcggggag ggccgaccgg
      361 acctctcccg ccgggccgct cggcccccct cgggatccgc agtccccctc cgctccagga
      421 ccgttcggcc cttccggtgg gggccggccg acccatcccg gcaggcccgg gcagcccgta
      481 gcgtcacggg caggaggcca tcggaggtga cgacgatgca ccggacgatc caggtcgacc
      541 caaccacaga cttcgaccac gcggccgtca ccgttcccgc tccacgcgag gagtccgagc
      601 acgacgccga ggtcgagctg atgcactcga tcgaccagct ggtgcggacc gcccgccacg
      661 atccggagct gaaggagctg atcctggcct gggaggacga gccgccgcag gacccgcggc
      721 cgcttctgcg gcacggcgag ttcacctgct cgggctgcca catggtgctg gaccggcgcc
      781 ggctcgccga cccggcctcg atgcgctgcg ccgattgcgc ctgatgggag gcgggagatg
      841 acccgacgag accgcgcgct gatcgtcagc gaggacgccg acctggtccg ccgctggacc
      901 gcctggctgg agcaggccga attcgacact gcgttctgcc cgggcccgct ggccgccttc
      961 gagtgcccgc gggcgaacgg cgactcctgt ccgcgccgtg agtgggccga tgtcgccgtg
     1021 gtggatgtcg ggcagctcgc ccggctcggc ctgagcggga tcggggccgc ccgctcgtgc
     1081 acgaccctcc ccgacgacgg ccgcacggtg gtcgccatgg agggcggaac gcatcaccgc
     1141 cacgggcgcc gccggccgac ggtcacgcat ccggtggagc ccgactcatt ggtcggcgtt
     1201 ctgcgcgcca ccgcgcaccg ccacccctcg gtggccctgc cgccgaaggc ctcctgaacc
     1261 ggcgcgaccg gggggacgat agcgcgcgcc cgctagcggt gctccgccgc gtccgcgtcg
     1321 gcccgggtga cgtggacggt ccccggcgga tgctgcggcg gcgcgtacaa ggagtagagc
     1381 tgcagctcgc cgtcgccggt gttgaccacg ttgtgccaca cgccggcggg gacgatgatc
     1441 gcccagtcct cccggacgtc gtgtgtctcc tcgacgcggt cctcctggct cgagagttcc
     1501 acgcgcccct tgccccgctc gacgcgcagg aactggtcga ggtgggggtg ccgttccctc
     1561 ccgatgtcct cgcccggccc gagccgcatc accgtcagct ggacgtgctc gcccgtgaac
     1621 agcacggtgc gaaaggtctc gttgtcgagc gtcacccgct cgatgtcgcc cacccatccc
     1681 agcatggagg tccccccttc cgggggccat tgtgcccgac gctcacgcct ccgtgtggtc
     1741 gtccgaggtg gactcgtcgt cggccagcag ccggtagagg ctcttgcgcg cctcgcgcag
     1801 gatctcgatg gcgcgcgcca cctgctgctc gtctccggtc tgcgcgacct gcatcgaggc
     1861 catcgccacg ccgcggccca cctcccacag cgcgacgacg gggctcgcgt cggcggccat
     1921 cgcccatggc gcttcgccgc cgcgccgggc cacctcctgc cggcccgcgt cggtgagctc
     1981 gaagacgcgg cgcccgtcgg cctcgctgct cgtgacgagt ccctcgtcct cgagcatctg
     2041 cagggtgggg tagaccgagc ccgggctcgg gcgccacacg ccccgcgtcc gctcggtcag
     2101 ctcctgtatc acctggtagc cgtgcatcgg gcgctcggcg agcagcgcga ggatcgccgc
     2161 gcggacgtcg ccccgcccgg cccgagcccc gcgcccgtag aaggggccgg ggaactcgcc
     2221 ccgcccccac ggcccgaacc cgcggccgaa gcggtaccgg ccgtggtgcc caccgtggtg
     2281 ccgcgcctcg tgccggtacc gctcatgggc ccagcgctcg tgcccgtgtg cccgctcctc
     2341 cgtgaagtcc cgcatcgctc ctccctcatc aggacctatc tcgtacgctc acgatatatc
     2401 gtgagcgacc gacagtcaag ggatcgtccc gggacggccg cggaaggacg ccgggccggc
     2461 cggccgtcgg gccgcgattc ggccacacgg ccgcacaatg aggggtgaat ccacgatatg
     2521 acacgatcgg aagggagccg tcgtgctgtt cagccgccac aagaccacgc tgcccgacgc
     2581 gagagaggcc ctgccgggcc gtgaccggcc ggccttcgcc atccccgagc gccacgaggt
     2641 gctcggcacg ccgatcctgc ccccgtaccc gcaggggctg caggtggcga cgttcgcgct
     2701 cgggtgcttc tggagcgcgg agcggctgtt ctggaagatc cccggggtct tcacgaccgc
     2761 cgtgggatac acgggtgggt acacgccgaa cccgacctac gaggaggtct gctcgggccg
     2821 gaccggccac gccgaggcgg tgctcgtggc attcgacccg gccacggtct cgtaccgcga
     2881 gctggttcgc gtgttcttcg aggagcacga cccgacccag gggatgcgcc aggggaacga
     2941 cgtcggcacg caatcccgct cggcgatcta cgtggccgac gaggagcagc gccgtgtcgc
     3001 cgagatggcc cgcgacgcgt acgacgccga cctccgcgcc cgcgggttcg acccggtgac
     3061 cacggagatc gacgtggccg ggcccttcta ctacgccgag gcctaccacc agcagtacct
     3121 gcacaaggtg ccgaacggct actgcggcct ggagggcacg ggcgtgcgct gctcgatccc
     3181 cgaggggagc gcctcgtgac cgacgagctg cgcgacaccg agctcccggc caccgaggag
     3241 gcatggcgcg cccgcctgac gcccgagcag taccgcgtgc tgcgcgaggc ggggaccgag
     3301 cgcgccttta ccggcgcgta cgtggacgcc gacgacgacg ggatctaccg ctgcgcggcg
     3361 tgcgggaacc cgctgttcga gagccggacg aagtaccact ccgggaccgg ctggccgagc
     3421 ttcacggcgg cggtctcccc ggacgcggtc gagctgctgg aggacacggc gtacgggatg
     3481 gtccgcactg aggtccgctg cgcgcgctgc cactcgcacc tcggccacgt cttcccggac
     3541 gggccgcccg aggcgggcgg ccagcgctac tgcatgaaca gcgtggcgct cgacctcgag
     3601 acgcggaacg gctgagggga cgggccgaag ctagcggaag ccgcgggcga tctcggcctc
     3661 ggcgacccgt ccgaccccga tcaggtacgc cgcctggcgc agggacagcc cgtcggcctc
     3721 ggcccgcgcc aggacctggt cggtggccgt ctccatgatc gtggcgagga cctcgttgac
     3781 gcgatccgcg gaccacgctt cgcgctgttg gttctggacc cactccaggt acgagaccgc
     3841 cacgccgccg ccggaggcga gcgtgtccgg cacgaccgag atcccgcggt cgagcaggat
     3901 cgcctcgccg tcgggggtga tcgggtagtt cgccacctcg agcaccgtgg cggccttcac
     3961 gtcgacggcg ttgtcctgcg tgacggcctc ctcgagcgcc gcgagcgcca ggaccgagca
     4021 gtccacgctt gggagctcct cgccggggat cgtctcggcg tccgcgaatc cgatcacgct
     4081 gccggtcgct tccttgtgcg ccaggaccgc gtcggggtcg agcccgcggt gcgacacgat
     4141 cgccccccgg ctgtccgagg cggccaccac cgggtagccc atcccggcgg cgatccgcgc
     4201 gagccacgag cctgcgtttc cgtagccctg gatcgccagc ggggcctcgg cgcgggccca
     4261 cccgcgcgcc cgggccagcc ggtcgaggca cagggcggca ccccgccccg tggcggcgtc
     4321 gcgcccgagc gagccgccga gcaccaccgg cttgccggtg atcaccgccg gggtggggcc
     4381 gtagcggcgg gcgtactcgt cgaccatcca gcccatgatc cgggggttgg tgttgacgtc
     4441 gggcgccggg acgtccacgt cgggcccgat caggtcggcg atcgcccgca tgaacgtccg
     4501 cgacaggcgc tcgagctcgg cctcggacag gagcttcggc tc
//
LOCUS       DASPWO010000308         2883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_387608, whole genome shotgun sequence.
ACCESSION   DASPWO010000308 DASPWO010000000
VERSION     DASPWO010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387608"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VEO00_07140"
     CDS             <1..620
                     /locus_tag="VEO00_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HYU57807.1"
                     /translation="AAAIGVGLPREAIVRGLKTFVLDPERNPGRMNLWELDGRVVVID
                     YAHNEAGLTGLIEVCRGLRPPGGEIWLTIATAGDRAPDIVTGMGYLAGRGSDHVGVAE
                     LLRYLRGRDRDELIALMRDGALDGGADAVSVYPDELAALRRTLRASRHGDVVAVTALG
                     MRPQVFAWLARRGAARLTPARVRALARRARRPATSRPASLARSAR"
     gene            617..1597
                     /locus_tag="VEO00_07145"
     CDS             617..1597
                     /locus_tag="VEO00_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57808.1"
                     /translation="MTGPLGVEVIELPGGGVIMAILALLIGAGLIALAYWVLPRPIAS
                     YVAGIGGFVLLALVVVLGQVSPVVPALLAAAVVFGAVAWLVRTDRRATAIGRFAAANG
                     LTHARRDARPSPFRASGLLMRIARRIGTAALLQGLGPGTAVTDADFRLFGVGDGRRAA
                     NVVTGAWRGAAVVEADLRWWDTKGYVLLIPLRRARRNSVVLVDLPVTIPRAAAIREDR
                     ARLIDKVLTHDVDLDLEAFNRAYVTKAADREFAFALFDQRLMQWLLDRPTLDGLEVNG
                     SRALLYCRRLPPADLPWLLDTARDFLERIPRVVRTRYGADGATTITGEVR"
     gene            1594..1908
                     /locus_tag="VEO00_07150"
     CDS             1594..1908
                     /locus_tag="VEO00_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HYU57809.1"
                     /translation="MTIPFREVVRFIGRSGRRVAVTAVGFALVAAGLAMLVLPGPGIL
                     VVIAGLAVLASEYAWARSLLDRAKEQARRARRRISRRGKGQPAEGPPEEPPAASDSGP
                     LP"
     gene            complement(1845..2438)
                     /locus_tag="VEO00_07155"
     CDS             complement(1845..2438)
                     /locus_tag="VEO00_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57810.1"
                     /translation="MTDADWDPLSALATLVRHRVRFVVIGGFAANILGSPMLTQDTDI
                     CYSRDPENLERLALALLELRARLRGPGVPEDLPFKLDATTLANGDHFTFMTSAGPLDI
                     LGTLAGVSGFDELDRAADDVEIAVGLVVRVPALDDLIRMKRAAGRPKDRLAVEELGAL
                     RDVRAQRAEEERQRRRAQGRGPESDAAGGSSGGPSAG"
     gene            complement(2435..2752)
                     /locus_tag="VEO00_07160"
     CDS             complement(2435..2752)
                     /locus_tag="VEO00_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYU57811.1"
                     /translation="MESAMVLRTARRRAGLTQRKLASIAAVPQSVVARIESGGVVPRV
                     DTLDRLLEACGEGLESVPRPGIGADRTLAWELLELSIPERLRRLVESSRAAARLSGRA
                     ASR"
BASE COUNT          359 a         1052 c         1061 g          411 t
ORIGIN      
        1 cggcggccgc gatcggcgtg ggcctgccgc gagaggcgat cgtccgcggg ctgaagacgt
       61 tcgtcctcga cccggagcgc aaccccgggc ggatgaacct gtgggagctc gacggccgcg
      121 tcgtggtcat cgactacgcg cacaacgagg cgggcctgac gggcctgatc gaggtgtgcc
      181 gcggcctgcg gccgccgggc ggggagatct ggctcacgat cgccaccgcc ggcgaccgcg
      241 cgcccgatat cgtcaccggg atgggctacc tggccgggcg aggctccgac cacgtcggag
      301 tggcggaact gctgcgctac ctccgggggc gcgaccgcga cgagctgatc gccctgatgc
      361 gcgacggcgc cctcgacggc ggcgccgacg ccgtatccgt ctatccggac gagctggccg
      421 ccctccgccg gaccctgcgc gcctcgcggc acggcgacgt cgtcgcggtg acggcgctcg
      481 ggatgcgccc gcaggtgttc gcctggctgg cgcggcgcgg ggcggcccgc ctgacccccg
      541 cgcgcgtcag ggccctggcc cgccgggccc ggcgcccggc cacgtcgcgt ccagcgagcc
      601 tcgcccggag cgcccggtga ccggcccact cggggtcgag gtgatcgagc tccctggggg
      661 cggcgtcatc atggcgatcc tggcgctcct gatcggcgcc ggcctgatcg cgctggcgta
      721 ttgggtgctg ccccggccga tcgcttccta cgtcgcgggg atcggcgggt tcgtcctgct
      781 cgcgctcgtc gtggtgctcg gacaggtgag cccggtggtg ccggccctgc tcgcggccgc
      841 ggtcgtgttc ggcgcggtgg cctggctggt ccggaccgac cggcgggcca cggccatcgg
      901 tcgcttcgcc gccgccaacg gcctgaccca cgcgcgccgg gacgcgcggc cgtcgccgtt
      961 ccgagcctcg ggcctgctca tgcgtatcgc ccgccgcatc ggtacggccg ccctgctgca
     1021 gggcctcggg ccggggacgg cggtcaccga cgccgacttc aggctgttcg gcgtgggaga
     1081 cgggcgccgc gctgcgaacg tggtcaccgg ggcctggcgt ggtgcggcgg tcgtcgaggc
     1141 cgacctgcgc tggtgggaca cgaagggata cgtcctcctg atcccgctgc ggcgggcccg
     1201 gaggaactcc gtggtgctgg tcgacctgcc ggtcacgatc ccccgggcgg ccgccatccg
     1261 tgaggatcgc gcgcgcctga tcgacaaggt gctgacccac gacgtggacc tcgacctcga
     1321 ggcgttcaac cgggcctacg tgacgaaggc cgccgaccgg gagttcgcgt tcgcgctgtt
     1381 cgaccagcgc ctgatgcagt ggctgctcga ccgcccgacc ctggacggtc tcgaggtcaa
     1441 cggctcgcgc gcgctgctct actgccggcg cctgccgccg gctgacctgc cctggttgct
     1501 cgacacggcg cgggacttcc tggagcggat tccgcgcgtg gtccgcacgc gctacggtgc
     1561 ggacggcgcc acgacgatca ccggggaggt ccggtgacca tcccgttccg tgaggtcgtt
     1621 cgcttcatcg gacgcagcgg caggcgcgtc gcggtgaccg cggtcgggtt cgcgctggtg
     1681 gcggccggtc tcgcgatgct cgtcctcccg gggccgggga tcctggtggt gatcgccggt
     1741 ctggccgtgc tcgcctccga gtatgcctgg gcccgttcgc tgctggacag ggcgaaggaa
     1801 caggcccgcc gcgcgcggcg gaggatctcc cgacgcggga agggtcagcc cgccgagggc
     1861 ccgcccgagg aaccgcccgc ggcgtcggac tccggccctc tcccctgagc ccggcggcgc
     1921 tgtcgttcct cctcggcccg ttgggcccgc acgtcgcgca gagccccgag ttcctccacc
     1981 gccaatcgat ccttgggccg gccggccgcc cgcttcatcc ggatgaggtc atcgagagcg
     2041 gggacccgga cgaccagtcc caccgcgatc tccacgtcat ccgccgcacg gtcgagctcg
     2101 tcgaaccccg agacgccggc gagcgtcccg aggatgtcga gcggtcccgc gctggtcatg
     2161 aacgtgaagt ggtcgccgtt cgcgagcgtc gtcgcatcca gcttgaaggg gagatcctcg
     2221 ggcacgccgg ggccgcggag gcgcgcccgg agctcgagca gggccaacgc caggcgctcc
     2281 aggttctccg gatcccgcga gtaacagatg tccgtgtcct gggtgagcat cggcgagccc
     2341 aggatgttgg cggcgaaccc tccgatgacg acgaagcgaa cgcggtgccg caccagtgtc
     2401 gccagcgcgc tgagtggatc ccagtccgca tcggtcaacg cgaggctgcc cttcccgaga
     2461 ggcgtgcggc agcgcggctc gactcgacca accgacgaag tcgttcgggg atcgagagct
     2521 cgaggagctc ccaggcaaga gttcggtcgg cgccgatacc cggccgcgga acggactcca
     2581 ggccctcccc acacgcctcg agcagacggt cgagggtgtc gacccgcggc accaccccgc
     2641 ccgactcgat ccgcgcgacc accgactgcg gcacggcggc gatcgacgcc agcttccgct
     2701 gcgtgaggcc ggcgcgccgc cgggcagtgc gaagcaccat cgcgctctcc accgcaccat
     2761 gatagcagtt agctatcaac agagcgctta ttcgatatca gaatccagga ggtccgggac
     2821 gatgtcgggg tcgccggtcg ggggctcgcc ggtggcgcgg ccgtcgccga ttaccgcggc
     2881 gcc
//
LOCUS       DASPWO010000309         6301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_303866, whole genome shotgun sequence.
ACCESSION   DASPWO010000309 DASPWO010000000
VERSION     DASPWO010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303866"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..502)
                     /locus_tag="VEO00_07165"
     CDS             complement(5..502)
                     /locus_tag="VEO00_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HYU57812.1"
                     /translation="MSERRQVYVAVVGPSEANAEEAGWAEAIGRGLARAGAVVLCGGL
                     GGAMDAVARGAESEGGLVVGILPGPGREGSSRHLTVALPTGLGETRNALIVRAADTVI
                     AVSGEFGTLSEIALALRLGVPVVGLGTWRLSKPGQEDAVDPIERAEDPEDAVRRALAV
                     AEGSS"
     gene            complement(521..2785)
                     /locus_tag="VEO00_07170"
     CDS             complement(521..2785)
                     /locus_tag="VEO00_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYU57813.1"
                     /translation="MDARRFLDRLTADPEAMPSLAHVETLAARDPRPEPVEGLPETLS
                     KRLAFLGIEGLYQHQRAALEALDAGDHVIVATGTASGKTLVYNLAFARTALADPKATA
                     LYLFPTKALARDQLRQVRELRLPQVKAAVYDGDTPQAERPMIRRNANLVMTNPDMLHA
                     SVLPGHARWADFFLRLAYVVVDEAHVARGVFGSHVAMVLRRLRRLVAHYGGDPRFVMA
                     SATVGNPAELAERLAGVPFRAVTEDASPRGEKLFALWNPPLLDQETGARRSALAETSF
                     LLERLIEGGVRSIAFVRSRRAAEVLADMTRKALAPIDRDRVKAYRAGYLPEERRALER
                     ALADGELLAVATTSALELGIDVGALDAALLAGYPGTRASMWQQAGRAGRRGESSLAVL
                     VAQDDPLDQYLVNHPDDLFAKPAEAAVIDPSNPYVVKPHLACAAREQPLRSEELAWFG
                     EEAPRLAEELRAAGALVENRGVLHHRGRESPHRLVDIRSASGALFAIVDEDTGALIGT
                     VDEARAFFQVHPGAIYLHQGEQYEVSRLDLAGRVALVARSNVDWYTRVRDVTDVRVLR
                     ALETEPFGSVEASFGELAVTVQVVGFQRIHVFTGDPIDVQPLALPQQDLVTKGLWWSI
                     PHAVLDRASVTPVQVPGAVHAAEHAAIGLLPLVATCDRWDVGGLSTPLHPDVGQTAIF
                     IYDGYPGGAGITERGFRSGTRLVTATLEALRLCSCRDGCPSCVQSPKCGNGNEPLDKR
                     AAIRLLSAMLEEER"
     gene            complement(2807..4153)
                     /locus_tag="VEO00_07175"
     CDS             complement(2807..4153)
                     /locus_tag="VEO00_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57814.1"
                     /translation="MRTLRRLAGFVASHHPLEGGLLSKVWPFPAVVGATLSIAWATEV
                     AAFFISRGLALAILAFLQVSPEFAVEAVLAKNAAQDPHQLQFVTANFTGSNRLLVGAA
                     LPVIFLIGRRVLSRQGRWPGHLQISAHHAVEVIALTLPSVYSLIWVLRGGLGILDTVV
                     LVGMFAGYLWLLSRMPAAEAEEVELLRGVPKRVMDTGNRSLQRTFAIGAFLLGGLVLL
                     FAAEPFVEGMKSIGEFIHVPPYLLLQWIAPLLSEFPEFFTVVYWSRQASVEQAFMNIV
                     AAKINQWTLLIAMIPLVFTITHLVRNSGGFTITFDYQQKIEILLTALQGLFAAACMFK
                     FRFLRWEAYALLGLWLFQLFDPVIDPYLQFLPSVFGGTTPAPELEKIIIREYTSIVFM
                     ALIVFELVRYRRHFRLFRSFADVWRRYGPGGSDRSGHGAQLGDTSVTGQARVSRES"
     gene            complement(4164..5072)
                     /locus_tag="VEO00_07180"
     CDS             complement(4164..5072)
                     /locus_tag="VEO00_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HYU57815.1"
                     /translation="MRRSLAVLAVVLAVAGSAAGGLALRAQSPARAQDAAPAGGESSG
                     SFVPPAAPGLITILVLGSDARPGEPIARLRSDSIHLVTINPRTGAGTVLGFPRDSYVA
                     IPGRGSARINEAMVYGGPQLTEATIESLTGIHIDYYVLASFQGFSDLVDGIGGIVVDV
                     PYDMQDPYSGANFRKGRTKLNGTRALAFARDRHSVPGGDFGRSENQGRLLLAALRQLR
                     QDFAVAPASVTKWVVVAARHVTTDLTIPELFRLGAAATRIRPSRVTNVVVPGSVGMVG
                     SASVVFVAPSARALFADIRDDGAVNG"
     gene            complement(5069..5728)
                     /locus_tag="VEO00_07185"
     CDS             complement(5069..5728)
                     /locus_tag="VEO00_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57816.1"
                     /translation="MKHRTRSPRRRGATIVLGLAIAAAVTASAWLLLDGPSGGASRTS
                     PTPPAVEFAFTLDKMRVNAVPEPWNNERARRAAQAVRAALTSFYVTGFVDPGAWAGGE
                     FPEAFAGFDKAAAGRARRDLDALTLGRFASSLDWVQPDDSHLVVTVLLDAHNRAVGAV
                     AEVRFVATAHRAAGGTLPIENRANYLLRPGGGGWTVFSYEATTRVGGADPSSGATEAT
                     A"
     gene            5838..6173
                     /locus_tag="VEO00_07190"
     CDS             5838..6173
                     /locus_tag="VEO00_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYU57817.1"
                     /translation="MDLEVEIGQRNGRSVIALRGEIDVYTAPRLREALIDQVEAGASD
                     ILVDMTRVEFLDSTGLGVLVGGLSRVKATGGSLEIVATDDKILKIFDITGLSKVFPIH
                     ASVDAALGG"
BASE COUNT          933 a         2252 c         2309 g          807 t
ORIGIN      
        1 gtggtcagga ggagccctcg gcgacggcca gggcgcggcg gacggcgtcc tcgggatcct
       61 cggcgcgctc gatcgggtcc accgcgtcct cctggccggg cttcgagagg cgccatgtcc
      121 cgaggccgac caccggcacc cccaggcgca gcgccagcgc gatctccgac agcgtgccga
      181 actctccgct caccgcgatc acggtgtccg ccgcgcggac gatcagcgcg ttgcgcgtct
      241 cgccgaggcc cgtcggaagg gccaccgtga ggtgccggct gctgccctcg cggcccgggc
      301 cggggaggat gccgaccacc aggcctccct cgctctcggc gccgcgcgcc accgcgtcca
      361 tcgctccgcc gagcccgcca cagaggacca ccgccccggc gcgagccagg ccgcgcccga
      421 tcgcctccgc ccagccggcc tcctcggcgt tcgcctccga cggcccgacg acggcgacgt
      481 agacctggcg acgctcgctc accgttcgct caccgttcgc tcaccgttcc tcctccagca
      541 tggcggagag caggcggatg gcggcgcgct tgtcgagcgg ctcattgccg ttgccgcact
      601 tcggcgactg cacgcacgac gggcagccgt cgcggcagga acagaggcgc agggcctcca
      661 gggtggccgt gaccagccgg gtgcccgacc ggaacccgcg ctccgtgatc ccggcgccgc
      721 cggggtagcc gtcgtagatg aagatcgccg tctggccgac gtcggggtgc agcggcgtcg
      781 agaggccccc gacgtcccag cggtcgcacg tcgcgacgag tggcagcagg ccgatggctg
      841 cgtgctcggc ggcgtgcacc gcccccggga cctggaccgg cgtcaccgag gcgcggtcga
      901 gcacggcgtg cgggatcgac caccagaggc ccttcgtcac gaggtcctgc tgcggcagcg
      961 ccagcggctg cacgtcgatc gggtcgccgg tgaagacgtg gatgcgctgg aacccgacca
     1021 cctgcacggt gacggccagt tccccgaacg acgcctcgac cgacccgaac ggctcggtct
     1081 ccagcgcccg gagcacgcgg acgtcggtca cgtcacgcac ccgcgtgtac caatccacgt
     1141 tgctgcgggc gacgagggcc acgcgaccgg cgaggtcgag gcggctgacc tcgtattgct
     1201 cgccctgatg caggtagatc gccccggggt ggacctggaa gaacgcgcgc gcctcgtcga
     1261 ccgtgccgat cagcgcgccg gtgtcctcgt cgacgatcgc gaagagcgcg ccggacgccg
     1321 agcggatgtc caccagccgg tgcggcgact cccgaccgcg gtggtgcagc acgccgcggt
     1381 tctccaccag cgcgccggcg gcccgcagct cctcagccag ccgcggcgcc tcctccccga
     1441 accacgcgag ctcctccgac cgcagcggct gttcgcgcgc ggcgcaggcc aggtgcggct
     1501 tgaccacgta ggggttggac gggtcgatca ccgcggcctc ggcgggcttc gcgaacaggt
     1561 cgtcggggtg gttcaccagg tactggtcga gtgggtcgtc ctgcgccacc agcacggcca
     1621 gggagctctc gccacgccgc ccggcgcgcc cggcctgctg ccacatcgag gcgcgcgttc
     1681 ccggatagcc ggccaggagc gccgcgtcga gcgcgccgac gtcgatcccc agctcgaggg
     1741 cgctcgtcgt cgcgaccgcc agcagctcgc cgtcggccag cgcgcgctcc agcgcccgtc
     1801 gctcctcggg caggtacccg gcccggtacg ccttgacccg gtcccggtcg atgggcgcga
     1861 gcgccttgcg ggtcatgtcg gccagcacct cggcggcccg gcgggagcgg acgaacgcga
     1921 tcgagcgcac tccgccttcg atcagccgct cgagcaggaa ggatgtctcg gccagggcgg
     1981 accgccgggc gccggtctcc tggtcgagca gcggcgggtt ccacagcgcg aagagcttct
     2041 cgccgcgcgg cgaggcgtcc tcggtcaccg cgcggaacgg gacgcccgcc agccgctcgg
     2101 cgagctcggc ggggttcccg acggtggcgc tcgccatcac gaaccgcggg tcgccgccgt
     2161 agtgcgccac caggcgccgt agccggcgca ggaccatcgc cacgtgcgac ccgaacacgc
     2221 cgcgggcgac gtgcgcctcg tccaccacga cgtaggcgag ccgcaggaag aaatcggccc
     2281 agcgcgcgtg ccccggcagg acgctggcgt gcagcatgtc ggggttcgtc atcaccaggt
     2341 tggcgttgcg ccggatcatc ggccgctccg cctgcggcgt gtcaccgtcg tagacggcgg
     2401 ccttcacctg cggcagccgg agctcacgca cctgccgcag ctggtcgcgc gccagggcct
     2461 tcgtggggaa caggtagagc gcggtggcct tggggtcggc gagtgccgtc cgggcgaacg
     2521 ccaggttgta gacgagcgtc ttcccgctgg cggtgccggt ggccacgatg acgtggtcgc
     2581 cggcgtcgag ggcctcgagc gccgctcgct ggtgctggta gagaccctcg atcccgagga
     2641 acgccagccg cttcgacagc gtctcgggga ggccctcgac cggctcgggc cgcgggtcgc
     2701 gggcggcgag ggtctcgacg tgggccagcg aaggcatggc ctccggatcg gcggtcagcc
     2761 ggtccaggaa cctccgcgcg tccacgcagt gaatgctagg tcctggtcat gactcccgag
     2821 agacgcgcgc ctgtccggtg acgctcgtgt cgccgagctg tgcgccgtgg ccggagcggt
     2881 cggagccgcc ggggccgtag cggcgccaga cgtcggcgaa ggagcggaac agccggaagt
     2941 gccgccggta gcggacgagc tcgaagacga tcagcgccat gaacacgatg ctcgtgtact
     3001 cgcggatgat gatcttctcc agctccggcg cgggtgtggt gccgccaaac acggagggga
     3061 ggaactgcag gtacgggtcg atcaccggat cgaacagctg gaacagccac agcccgagca
     3121 gcgcgtaggc ctcccagcgc aggaaccgga acttgaacat gcaggcggcc gcgaacaggc
     3181 cctgcagcgc cgtcagcagg atctcgatct tctgctggta gtcgaacgtg atcgtgaacc
     3241 cgcccgagtt ccggaccagg tgcgtgatcg tgaacacgag cgggatcatc gcgatgagca
     3301 gcgtccactg gttgatcttc gcggcgacga tgttcatgaa ggcctgctcg acgctcgcct
     3361 gccgactcca gtagacgacc gtgaagaact ccgggaactc cgacagcagc ggcgcgatcc
     3421 actgcagcag gaggtacggc gggacgtgga tgaactcgcc gatcgacttc atcccctcga
     3481 cgaacggctc ggcggcgaac aacaggacga gcccgccgag aaggaacgcg ccgatcgcga
     3541 aggtccgctg cagcgaccgg ttgcccgtgt ccatcacccg tttcggcacg ccccgcagca
     3601 gctcgacctc ctccgcctcg gcggccggca tcctggagag caaccagagg tacccggcga
     3661 acatccccac gagcacgacg gtgtcgagga tcccgaggcc tccgcgcagc acccagatca
     3721 gcgagtagac cgatggcagg gtcagcgcga tcacctcgac ggcgtggtgg gccgagatct
     3781 gcaggtgccc gggccagcgg ccctgccggg aaaggacgcg gcgcccgatc aggaagatca
     3841 ccgggagcgc cgccccgacc agcaaccggt tcgagccggt gaagttcgcc gtgacgaact
     3901 gcagctggtg gggatcctgc gcggcgttct tcgccagaac ggcctcgacg gcgaactccg
     3961 gcgagacctg caggaaggcg aggatcgcga gcgccaggcc gcgggagatg aagaaggccg
     4021 ccacctcggt ggcccaggcg atcgacagcg tcgcgccgac aaccgccggg aacggccaga
     4081 ccttggacag caggccgccc tcgagcgggt gatgcgatgc gacgaacccc gccagccggc
     4141 ggagcgtcct cacccgttct ccctcacccg ttcaccgccc cgtcgtcgcg gatgtcggcg
     4201 aacagggctc gggcgctcgg ggcgacgaag accacgctcg ccgagccgac catgccgacc
     4261 gacccgggca cgacgacgtt tgtcacgcgg ctcgggcgga tgcgcgtcgc cgccgcgccc
     4321 aggcggaaca gctcggggat ggtcaggtcg gtggtgacgt gccgggcggc gaccacaacc
     4381 cacttcgtca ccgacgccgg cgccactgcg aagtcctggc gcagctggcg cagggccgcc
     4441 agcagcagcc tgccctggtt ctccgagcgg ccgaagtcgc cgccggggac gctgtggcgg
     4501 tcgcgggcga acgccagcgc ccgggtgccg ttcagcttgg tgcggccctt gcggaagttg
     4561 gcccccgaat aggggtcctg catgtcgtag gggacgtcga ccacgatgcc gccgatgccg
     4621 tccaccaggt cggagaaccc ctggaacgac gccaagacgt agtagtcgat gtggatcccg
     4681 gtcagggact cgatcgtcgc ctcggtcagc tgcgggccgc cgtagaccat cgcctcgttg
     4741 atccgggcgc tcccccgccc cgggatcgcc acgtagctgt cgcgcgggaa cccgagcacc
     4801 gtccccgccc ccgtccgcgg attgatggtg accaggtgga tcgagtcgga acgcaaccgc
     4861 gcgatcggct ctcccggccg ggcgtcgctg ccgaggacga ggatggtgat caggcccggc
     4921 gcggccgggg ggacgaagga cccggaggac tcgccgcccg cgggggcggc gtcctgcgcc
     4981 cgggccgggg actgcgcgcg cagggccagg ccgccggcgg ccgagccggc cacggccagg
     5041 accaccgcga gcacggcgag cgaccgcctc atgccgtcgc ctcggtggcg ccgctcgagg
     5101 gatcggcccc gccgaccctg gtggtggcct cgtaggagaa cacggtccag ccacccccgc
     5161 ccggccgcag caggtagttc gcgcgattct cgatcgggag cgtcccaccg gcagcgcgat
     5221 gggcggtggc gacgaagcgg acctcggcca ctgcgcccac ggcccggttg tgtgcatcga
     5281 gcagcacggt gaccaccagg tgcgagtcgt cgggctggac ccaatcgagg ctcgatgcga
     5341 accgcccgag cgtcagcgcg tcgaggtcgc gccgggcgcg gcccgcggcc gccttgtcga
     5401 acccggcgaa agcctccggg aactccccgc cggcccaggc gccggggtcg acgaaacccg
     5461 tgacgtagaa ggaggtcagg gcggcgcgta cggcctgtgc cgccctgcgg gcgcgctcgt
     5521 tgttccaggg ctcggggacg gcgttcacgc gcatcttgtc gagcgtgaac gcgaactcca
     5581 ccgcgggagg ggtgggcgac gtcctcgacg cgccgccgct cggcccgtcc aggaggagcc
     5641 aggcagacgc cgtgacggcg gccgcgatcg cgaggccgag cacgatggtg gcgccgcgcc
     5701 gccgggggga gcgagtccgg tgcttcacgc ggggggcgac gctagcgggc gccgcgcggg
     5761 tttggcaagc gaaagtgagg atcggccgac gcgcaggggc gatgggatcc gcccggtatg
     5821 ggtagactcc cggagtcatg gatctcgagg tcgagatcgg ccagcgaaac ggccgcagcg
     5881 tgatcgcgct gcgcggcgag atcgacgtct acacggcccc ccggctgcgt gaggccctga
     5941 tcgaccaggt ggaggcgggc gcctcggaca tcctcgtcga catgacgcga gtggagttcc
     6001 tcgactcgac cggcctcggc gtcctggtgg gcggcctctc ccgggtgaag gcgacggggg
     6061 ggtcgctcga gatcgtcgcc accgacgaca agatcctgaa gatcttcgac atcaccgggc
     6121 tgtcgaaggt gttcccgatc catgcctcgg tcgacgccgc gctgggcggg taggtggcgg
     6181 gcgtgtccgg ggctcccggc gaggggcttg gggggaccga cgagaccgtc cagatctcgg
     6241 tcccggcgcg gccggagttc gtcggcgtgg tgcgccacgt cctcggcgcg ctggcgcgga
     6301 t
//
LOCUS       DASPWO010000310         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_387892, whole genome shotgun sequence.
ACCESSION   DASPWO010000310 DASPWO010000000
VERSION     DASPWO010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387892"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VEO00_07195"
     CDS             complement(<1..438)
                     /locus_tag="VEO00_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYU57818.1"
                     /translation="MQADTFWFQASDGTWLDGATLGTGSVGVVLAHEYPADLCGWWPY
                     AVYLSRHGLRILLFDFRCFGASTCPGGGSLLSDVEGAVRVLRADDVRDVFLLGGSLGG
                     TVVMMAGVEITPRVEGVVSLSGEADLGFLVGDVALNARSAVGDL"
     gene            complement(715..1710)
                     /locus_tag="VEO00_07200"
     CDS             complement(715..1710)
                     /locus_tag="VEO00_07200"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HYU57819.1"
                     /translation="MSDRSRRSGPVPLVRVLRSGLEEAVHLGSVAVTDAAGTLVARAG
                     DPGRATFARSTMKPLQATVSLSLIGEDLTDREIAVMCASHNGEPVHVEAVAALLARAR
                     LGFDALRTPSMAPWDQEAALVAGRRAPEFSDCSGKHAGMLVACARQGWDLASYRDADH
                     PIQRRVLAAVVSASGQREVAVGVDGCGVPVHWIPVRSLGALYARLGRPEALGELASFA
                     ARALQAMGREPYLVAGRGRVCTAVMEAVPGVVVKVGAEGLICAALTGDGLGVAVKITD
                     GGARAAGPALLHTLDLLGAMPPGAAGALERWIHPPVLGGGEPRGHLEADFDLTFA"
     gene            complement(1736..2497)
                     /locus_tag="VEO00_07205"
     CDS             complement(1736..2497)
                     /locus_tag="VEO00_07205"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HYU57820.1"
                     /translation="MVQQATPGAPGDPALRELEAVRRQLMVFAQEMNIVYRDARDRSA
                     QLENVLNQLQAAHLATMKTLAFVVEAKDLTTRGHLERTHRYGVALARLMDPDLARRPQ
                     LGYGFLLHDIGKVGVPEGILGKEGPLTADEMEVMKTHTVLGAQIVAPMEFLGEAVDVI
                     KYHHERWDGCGYPHGLRREAIPLSARIFAVVDAFDAMTSDRPYREALRVEEAAAEIAR
                     GSGRQFDPEAVEAFHILIDRLELAVAGPPTLSVVS"
BASE COUNT          389 a          960 c          919 g          322 t
ORIGIN      
        1 cagatcgccc accgccgatc gagcgttcag cgccacgtcg cccaccagga agccgagatc
       61 ggcttccccg gacagactga ccacgccttc cactcggggc gtgatctcca ccccggccat
      121 catcacgacg gtgccgccca aggatccccc gagcaggaac acgtcgcgaa cgtcgtctgc
      181 ccgaagcacc cgaaccgcac cctcgacgtc gctgagcagg ctgccacctc ccggacaggt
      241 cgacgcgccg aagcacctga agtcgaacag caggatgcga aggccatgac gactgaggta
      301 cacggcatac ggccaccaac cgcagaggtc ggccgggtac tcgtgcgcca gcacgacacc
      361 cacggagccc gtccccagcg tcgccccgtc gagccaggtc ccgtcactcg cctggaacca
      421 gaacgtgtcg gcctgcacgt tcggggatcc gcatcggcgc gcgaccggtt ggagcggcgc
      481 ctgggtcgcg gcgggcgacg gtgacggggt cgccgaggca ccgaccggtc cgttgcccgg
      541 ccccttggac gtgcagctga cccccgccac gagcgtcgaa acgatcacgg cgctcgcgat
      601 cgaccatcgg cggttcacat cccaggagta cgggctgcgc ccgggtcgcg gattcgggcg
      661 gggaggtgga ttgcccggcc ggccccacgg tcgacaggct cgtgagcggc gcccctacgc
      721 gaaggtgagg tcgaagtcgg cttcgaggtg gccgcgcggc tccccgccac ccagcaccgg
      781 cgggtggatc cagcgctcca gggcgccggc agcgccgggc ggcatggcgc cgaggaggtc
      841 cagcgtgtgg agcagggccg gcccggccgc tcgcgcgccg ccgtcggtga tcttcaccgc
      901 cacgcccagg ccgtcgccgg tgagcgccgc gcagatcagg ccctcggcac cgaccttgac
      961 caccacgccg ggaaccgcct ccatcaccgc ggtgcagacg cggccgcggc ccgccaccag
     1021 gtagggttca cggcccatcg cctggagggc ccgcgccgcg aacgaggcga gctccccgag
     1081 ggcctcgggc cggccgagcc gcgcgtagag cgccccaagc gagcgcaccg ggatccagtg
     1141 gacggggacg ccgcacccgt ccacgccgac ggccacctcg cgctgcccgg aggccgacac
     1201 cacggccgcc agcactcgac gctggatcgg gtggtcggcg tcccggtagg aggccaggtc
     1261 ccaaccctgc cgggcgcagg ccacgagcat cccggcgtgc ttcccggagc agtcggagaa
     1321 ctccggggcc cgccgcccgg cgacgagcgc ggcctcctgg tcccagggcg ccatcgaggg
     1381 cgtgcgcagg gcgtcgaacc cgagccgcgc ccgcgccagg agggctgcga cggcctccac
     1441 gtggacgggc tcgccgttgt ggctggcgca catcacggcg atctcgcggt cggtgaggtc
     1501 ctccccgatc agcgagagcg agaccgtcgc ctgcaggggc ttcatggtcg agcgagcgaa
     1561 cgtggcgcgg ccgggatcgc cggcccgggc cacgagcgtc cccgccgcgt cagtcaccgc
     1621 cacggacccg aggtgaaccg cctcctcgag ccccgagcgc agcacgcgaa cgagcggcac
     1681 cgggccggaa cggcgggaac ggtcgctcac ggccgcagcg tagttgaggc ggagctcagg
     1741 aaacgacgga gagggtgggc gggccggcca cggccagctc caggcggtcg atcaggatgt
     1801 ggaaggcctc tacggcctcc gggtcgaact gccgcccgga tccacgggcg atctcggccg
     1861 ccgcctcctc gaccctcagc gcttcgcggt agggtcggtc gctggtcatg gcgtcgaagg
     1921 cgtccaccac cgcgaagatc cgggccgaca gcgggatcgc ttcgcgccgc aggccgtgcg
     1981 ggtagccgca gccgtcccat cgctcgtggt ggtacttgat gacgtccacg gcctcgccca
     2041 ggaactccat cggtgccacg atctgtgccc cgagcacggt gtgggtcttc atcacctcca
     2101 tctcatcggc ggtgaggggc ccctccttgc cgaggatgcc ttcgggcacg ccgaccttcc
     2161 cgatgtcgtg caggaggaag ccgtagccga gctgcggccg tcgcgccagg tcgggatcca
     2221 tcagcctcgc cagcgcgacg ccgtagcgat gcgtccgctc gaggtggccg cgcgtggtga
     2281 ggtccttggc ctcgaccacg aacgccagcg tcttcatcgt ggcgaggtgc gcggcctgca
     2341 gctggttcag gacgttctcg agctgcgcgg accggtcgcg cgcgtccctg tagacgatgt
     2401 tcatctcctg cgcgaagacc atcagctggc ggcgaacggc ctccagctcg cgaagcgcgg
     2461 ggtcgccggg cgctcccggc gtcgcctgct gcaccatgag gcgtccatcc tggcggaggg
     2521 agccaacgga ggcaatggtg cgaacgggcc ggtgtccgtc ggccgttcgg catcgctcga
     2581 acggccgacc
//
LOCUS       DASPWO010000311         3462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1231774, whole genome shotgun sequence.
ACCESSION   DASPWO010000311 DASPWO010000000
VERSION     DASPWO010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231774"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="VEO00_07210"
     CDS             complement(<1..1408)
                     /locus_tag="VEO00_07210"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983910.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HYU57821.1"
                     /translation="MATKERDVRTGRGGLTFRRYFTTEGTHPFDEVEWEIRDAVIPNH
                     KDGGNAFEQRGVEFPKSWSQNATNIVAQKYFRGPLGMPERENSVRQMVGRVVDTITGW
                     GVKDGYFANDGDARTFSDELTHILVNQKAAFNSPVWFNVGVEDHPQCSACFILAVDDT
                     MSSILNWYVEEGTIFKGGSGSGINLSSIRSSKERLAGGGTASGPVSFMRGADASAGTI
                     KSGGKTRRAAKMVILNAEHPDVRDFIWCKAVEERKARVLRDAGFDMDLDGKDSYSTQY
                     QNANNSVRVTDEFMQAYLDDRDWKLKAVLSNEAVETVRARDLMREIAEAAWECADPGM
                     QYDTTINDWHTAPASGRINASNPCSEYMHLDNSACNLASLNLMKFVDDDMNFDIAAFK
                     HAVEVVFTAQEILVGNSSYPTEKIGRNAKAFRQLGLGYANIGALLMARGLPYDSDAGR
                     AWAGAITALMTGRAYRKSA"
     gene            complement(1625..2131)
                     /gene="nrdR"
                     /locus_tag="VEO00_07215"
     CDS             complement(1625..2131)
                     /gene="nrdR"
                     /locus_tag="VEO00_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HYU57822.1"
                     /translation="MLWFVGRPSVRCPWCGREQDRVVDSRTAESGAAIRRRRQCAGCG
                     RRFTTYERVEEPALVVIKRDGAKEPYSREKVVAGISKAIKNRPVSPEQVEQMAARTEE
                     RLRRKGPEVTTQDVGLEVLKQLAKLDQVAYLRFASVYRDFQELTDFERELGLLLQKRQ
                     GRRARARG"
     gene            complement(2201..2527)
                     /locus_tag="VEO00_07220"
     CDS             complement(2201..2527)
                     /locus_tag="VEO00_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HYU57823.1"
                     /translation="MARTRVRWGRVGVLAVTLFGALNLAAAAAGAGGDARSASRPTPA
                     SVPVQRQAPVPHVVRPGETLWAIAARLAGPDRDPRPLMDRLAVENGLVGGNLQAGQTI
                     LLPAAG"
     gene            2631..3245
                     /gene="lexA"
                     /locus_tag="VEO00_07225"
     CDS             2631..3245
                     /gene="lexA"
                     /locus_tag="VEO00_07225"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HYU57824.1"
                     /translation="MDDLTARQRRILDYIRATVRDRGYPPTVREIGEAVGLTSSSSVH
                     SQLATLQRKGLLTRDPTKPRAIELHLPGSSRPDAVAVPVLGRIAAGAPVLAAEHVEDE
                     IALPRGLVPEGDLFALRVAGDSMTGAGILDGDLVVVEQRDDPRSGDIVAALLPGPAED
                     EATVKRLRRRDGRVSLEPENPAYEPIEMIEGRILGTVVAVLRTY"
     gene            complement(3239..>3462)
                     /locus_tag="VEO00_07230"
     CDS             complement(3239..>3462)
                     /locus_tag="VEO00_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007238173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57825.1"
                     /translation="RGDLTARLYREAEVLSNEAAPEGQRIRVRVPERLVGAVTPFLLH
                     SVSTGGGTTSRRAAPSGGVPGGEAPRVHQ"
BASE COUNT          488 a         1243 c         1186 g          545 t
ORIGIN      
        1 ctgcggactt gcgatatgcg cggccggtca tcagcgcggt gatcgccccg gcccaggccc
       61 gcccggcatc ggaatcgtag ggcagcccgc gcgccatcag gagcgcaccg atgttcgcgt
      121 agccgaggcc gagctggcgg aacgccttcg cgttgcgccc gatcttctcc gtcgggtagc
      181 tcgagttccc gacgaggatc tcctgcgccg tgaacacgac ctccaccgcg tgcttgaacg
      241 cggcgatgtc gaagttcatg tcgtcgtcga cgaacttcat aaggttcagg ctcgccaggt
      301 tgcacgccga gttgtcgagg tgcatgtact ccgagcacgg gttggaggcg ttgatgcggc
      361 ccgacgccgg cgcggtgtgc cagtcgttga tcgtggtgtc gtactgcatg ccggggtcgg
      421 cgcactccca ggcggcctcg gcgatctcgc gcatcagatc gcgggcgcgg acggtctcga
      481 ccgcctcgtt ggacaggacg gccttcagct tccagtcgcg gtcgtccagg taggcctgca
      541 tgaactcgtc ggtgacccga acggagttgt tcgcgttctg gtactgcgtc gagtacgagt
      601 ccttgccgtc gaggtccatg tcgaacccgg cgtcgcgcag gacccgcgcc ttgcgctcct
      661 ccaccgcctt gcaccagatg aagtcgcgca cgtcggggtg ctccgcgttc aggatgacca
      721 tcttcgcggc gcggcgggtc ttgccgcccg acttgatcgt cccggccgag gcgtcggccc
      781 cgcgcatgaa gctgaccggc ccggaggcgg tcccgccgcc ggcgaggcgc tccttcgagg
      841 aacggatcga ggacaggttg atgccggagc cggacccgcc cttgaagatc gtcccctcct
      901 cgacgtacca gttgaggatc gaggacatcg tgtcgtccac cgcgaggatg aagcacgccg
      961 agcattgcgg atgatcctcc acgccgacgt tgaaccagac cgggctgttg aaggcggcct
     1021 tctggttcac caggatgtgc gtcagctcgt ccgagaacgt ccgggcgtcc ccgtcgttcg
     1081 cgaagtagcc gtccttcacg ccccagccgg tgatcgtgtc gaccacccgg ccgaccatct
     1141 ggcgcaccga gttctcccgc tccggcatgc cgaggggccc ccggaagtac ttctgcgcca
     1201 cgatgttcgt cgcgttctgg ctccaggact tcgggaactc cacgccgcgc tgctcgaagg
     1261 cgttgccgcc gtccttgtgg ttggggatga ccgcgtcgcg gatctcccac tcgacctcgt
     1321 cgaacgggtg cgtgccctcg gtcgtgaagt accgcctgaa cgtcagcccg ccgcgcccgg
     1381 tccggacgtc acgttccttg gtggccacgg tgatccccct ttcggctccc ggcggggaag
     1441 cgaccatcgg aatgacaact ctgtgattct ccgtcgccgg acccgcggcg tcaagcccct
     1501 cgaaggaaca tattgtacct gggctcgtcg acaaccacaa catgttgtgt ttcgtgggga
     1561 aaggctgtgg acgggctgtg gaccgcctgt ggacggtcgg aggccgcttc ggctgcccgc
     1621 tcgctcagcc gcgagcgcgg gcacggcgac cctggcgctt ctgcagcagc aggcccagct
     1681 cacgctcgaa gtcggtcagc tcctggaagt ccctgtacac gctggcgaat cgcaggtagg
     1741 ccacctggtc gagcttcgcc agctgcttca ggacctcaag cccgacgtcc tgcgtggtga
     1801 cctcggggcc cttgcggcgc aggcgctcct cggtccgagc ggccatctgc tccacctgtt
     1861 cgggcgagac cgggcggttc ttgatcgcct tgctgatccc ggcaacgacc ttctcccggc
     1921 tgtacggctc cttcgcgccg tcgcgcttga tcaccacgag cgccggctcc tcgacccgct
     1981 cgtaggtggt gaaccggcgg ccgcacccgg cgcactggcg ccggcggcgg atggcggcgc
     2041 cgctctctgc cgtgcgcgag tcgaccacgc ggtcctgctc cctgccgcac caaggacacc
     2101 gcaccgacgg cctcccgacg aaccacaaca tgtggtggtt cgtccacggt agcaacgcac
     2161 atgtcgtggg gtcaagacgc ggcgcccggc gagcggggcc tcagccggcc gcgggaagca
     2221 ggatggtttg accggcctgc aggttcccgc cgaccagacc gttctcgacg gcgaggcggt
     2281 ccatcagcgg acgcggatca cggtccggcc ccgcgagccg agcggcgatc gcccagagcg
     2341 tctcccccgg gcggacgacg tgggggaccg gcgcctgccg ttggaccggc accgacgccg
     2401 gcgtggggcg ggaggcggac cgggcgtccc cgccggcgcc ggcggcggcg gccgccaggt
     2461 tcagcgcccc gaagagcgtg accgccagca cgcccacacg gccccaccga acgcgcgttc
     2521 gagccacgcc gcaaagtatg tcgaacacgt gttcgcctgt caagctcgaa caggtgtttg
     2581 attcggccgc gttccggtgc tagcgtcgcc gccggaggag gagacgagag atggacgacc
     2641 tgacggcacg gcagcgccgg atcctcgact acatccgcgc cacggtgcgc gaccgcggct
     2701 atcccccgac cgtccgcgag atcggcgagg cggtggggct caccagctcc tcgagcgtgc
     2761 acagccagct cgccacgctc cagcgcaagg gcctgctgac ccgcgacccc acgaagcccc
     2821 gggccatcga gctgcacctc cccggctcga gccgtccgga cgcggtggcg gtgcccgtcc
     2881 tcgggcggat cgccgccggc gccccggtgc tggcggccga gcacgtcgag gacgagatcg
     2941 cgctgccccg tggcctggtc cccgagggcg acctgttcgc gctgcgggtg gccggtgact
     3001 ccatgaccgg agccggcatc ctcgacggtg acctggtggt cgtcgagcag cgtgacgacc
     3061 cccgctccgg cgacatcgtc gccgcgctcc tgcccggccc ggcggaggac gaggccaccg
     3121 tcaagcgcct gcgccggcgc gacggccgcg tgtccctcga gccggagaac cccgcgtacg
     3181 agccgatcga gatgatcgag ggccgaatcc tcggcacggt cgtcgcggtc ctacgcacct
     3241 actgatgcac ccggggggct tcgccccccg ggaccccgcc ggacggggcc gctctccggg
     3301 aggttgtgcc tccgccggtg gacacggagt ggagcaggaa gggcgtgacg gcgcccacca
     3361 ggcgctcggg gacccggacg cggatccgct gcccctcggg cgccgcctcg ttcgacagga
     3421 cctcggcctc gcggtagagg cgggccgtca ggtcccctcg at
//
LOCUS       DASPWO010000312         3740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1231844, whole genome shotgun sequence.
ACCESSION   DASPWO010000312 DASPWO010000000
VERSION     DASPWO010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231844"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..702
                     /locus_tag="VEO00_07235"
     CDS             118..702
                     /locus_tag="VEO00_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015299050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HYU57826.1"
                     /translation="MFKRRTGDEKARTPPGQRVTRGWPILHAEPVPRFDPATWRFRVT
                     GQVERELELTWETFRALPSVTIESDIHCVTGWTKLDNTWEGAAFREIAERAGAKPNVT
                     HVVTSAPSGYTANLPIEAVMDDDALFAWAHDGQPLDPQHGGPLRLVVPKRYFWKSTKW
                     VIGVEFLDHDERGFWEVRGYHNDAHPFAEERYSW"
     gene            990..1976
                     /locus_tag="VEO00_07240"
     CDS             990..1976
                     /locus_tag="VEO00_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57827.1"
                     /translation="MPNPTVTVIGSYPRTPRAGEPFTLRTTLQAMDRGEATWEDVARA
                     QDQVVRDVVAEQAAAGVELVTDGQIRWDDAQTRFAAGLEGVTIGSLERYFDNNTYFRQ
                     PEIKGPISRRGPIVVDEFRFARAASPVPVKAVVIGPYTLAALSKRDDGLEFAALVREL
                     AVALSEEARDLAAAGATVIAFDEPALARPLGLPEPDPAALEDAFAALVDGVEATTVLQ
                     TYFGDVHPLGETVFEWPFDAFGLDLVAGPGNAGLLERLPAGKVLQAGVVDGRNTKLEP
                     VEEVASRLRWVAERVPLDRVWVAPSCGLEFMGREPAQRKLESLARAAREVGE"
     gene            1977..2945
                     /locus_tag="VEO00_07245"
     CDS             1977..2945
                     /locus_tag="VEO00_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HYU57828.1"
                     /translation="MEGLRTTTVGSFPKPDYLATARTAHARGELSREALQELERKATA
                     EWIAFQDEIGIDLLVDGEQARGDMVAFFAEALPSMELGGLVRSYGNRYYRKPIITGEL
                     RWTAPMTVEMWRFAQGLTERPVKGMLTGPYTMVEWSFDEHYPSRRDAVLDMAHVVRDE
                     AIALAMAGARYIQVDEPAIHTRPDEDFDLAVEALEVVVHGVDAEFHTHVCYGDVELIY
                     PAMLRLPVSQISLAFKNTDFAYFDLMRKDPWEGADVGLGVLDSHDHRTETVEEVVEGI
                     RGALELLPAERIWVEPDCGLKTRTVEETRDKLRVMAEAVRRLKGES"
     gene            3005..>3740
                     /locus_tag="VEO00_07250"
     CDS             3005..>3740
                     /locus_tag="VEO00_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYU57829.1"
                     /translation="MGDPSEPRERGGWRHVPALVVSLLFVIPLVVAVAGSLRDAGLPP
                     PRTVEVIPERLAVENYPEAYALVDLPRQVVNSLIVVAFAVPLTVLVASWAGFAISRMR
                     RRAATVVVALSLLALVVPATALLVGRFAVFRTLRLTDTFVPLVAPALIGTSPFYVLLY
                     AWTFRRLPADLFEAARLEGSTPFEQWRRVGMPLAKPVTAAVAALAFALTWGNLLDPLI
                     YLFDPSKYTVPLGLRALSGVDAPDLPL"
BASE COUNT          496 a         1306 c         1409 g          529 t
ORIGIN      
        1 agccgccgcg ctcgcggatc gcgcggcggg cgagcagctg gcgtcgctga tcgccgtgcg
       61 ccgcgaggac ctggctcgcc tctccggcga gtcggggagc gggtcgagcg agccgtgatg
      121 ttcaagcggc ggacgggcga cgagaaggcc cgcacgccgc cgggccagcg cgtcacgcgc
      181 ggctggccga tcctgcacgc cgagccggtt ccccgcttcg acccggccac gtggcgcttc
      241 cgggtcaccg gccaggtcga gcgcgagctg gagctcacct gggagacgtt ccgggcgctg
      301 ccgagcgtga cgatcgagtc cgacatccac tgcgtcaccg gctggacgaa gctcgacaac
      361 acctgggagg gcgccgcctt ccgcgagatc gccgagcggg cgggggcgaa gcctaacgtg
      421 acgcacgtcg tgacctccgc cccctccggc tacaccgcga accttccgat cgaggccgtg
      481 atggacgacg acgccctgtt cgcctgggcg cacgacgggc agccgctcga tccgcagcac
      541 ggcggcccgc tgcgtctggt ggtgccgaag cggtacttct ggaagagcac gaagtgggtg
      601 atcggcgttg agttcctcga ccacgacgag cgcggcttct gggaggtccg cggctaccac
      661 aacgacgccc atcccttcgc cgaagagcgc tactcctggt aggagtcgcg gtagcggcga
      721 cgcgccggac ggtcggttcc cggccgccgt ggtagattcc gcgccgtatc aggagcggcc
      781 cggcgtgccg ggcccggcaa cctgggacgg acacccgagg tgcctcccgc gcgagcgggg
      841 atgcggcccc ggcgacgggg caaccaagtg tccggcgcga gcgtcgcgac gcccgcgctg
      901 cgggtcgaca ggggcccgga ccgagcgcat ccccgctccg cccgaggccg tcgcgccgtt
      961 cctcgacgag gcgagcgaag gagaggcgag tgccgaaccc gaccgtgacc gtcatcggct
     1021 cctacccgcg cacaccgcgg gcgggagagc cgttcacgct gcgcaccacg ctgcaggcga
     1081 tggaccgcgg cgaggcgacg tgggaggacg tcgctcgcgc ccaggaccag gtggtgcgcg
     1141 acgtcgtggc cgagcaggcc gccgccggcg tcgagctcgt caccgacggc cagatccgct
     1201 gggacgacgc ccagacccgt ttcgcggcgg ggctggaggg cgtgacgatc ggaagcctgg
     1261 agcggtactt cgacaacaac acgtacttcc gccagccgga gatcaagggc ccgatctccc
     1321 gtcgcggccc gatcgtcgtc gacgagttcc gcttcgcccg ggcggcgagc ccggtgccgg
     1381 tgaaggccgt ggtgatcggc ccctacacgc tcgccgccct gtcgaagcgc gacgatgggc
     1441 tggagttcgc tgcgctggtc cgagagctgg cggtggcgct ctccgaggag gcgcgcgacc
     1501 tcgccgcggc cggggccacc gtgatcgcgt tcgacgagcc ggccctcgcc cgcccgctgg
     1561 gcctgccgga gcccgacccg gcggccctcg aggacgcgtt cgccgcgctc gtcgacggcg
     1621 tcgaagcgac gaccgtgctg cagacctact tcggcgacgt ccaccctctc ggcgagacgg
     1681 tcttcgagtg gccgttcgac gcgttcggcc tggacctggt ggccgggccg gggaacgcgg
     1741 gcctgctcga gaggctcccg gccgggaagg tcctgcaggc cggggtcgtg gacggacgga
     1801 acacgaagct cgagccggtc gaggaagtgg cctcgcggct ccggtgggtg gccgagcggg
     1861 tccctctcga tcgtgtgtgg gtcgcgccgt cgtgcggcct ggagttcatg ggacgggagc
     1921 cggcgcagcg caagctcgag agcctggcgc gcgcggcccg ggaggtgggg gagtagatgg
     1981 aaggcctgcg cacgaccacg gtcggatcat tccccaagcc ggactatctg gcgacggcgc
     2041 gcaccgcgca cgcgcgcggc gagctgtccc gcgaggcgct ccaggagctg gagcggaagg
     2101 cgaccgctga gtggatcgcg ttccaggatg agatcgggat cgacctgctc gtggacggcg
     2161 agcaggcccg cggggacatg gtggcgttct tcgccgaggc gctcccgagc atggaactcg
     2221 gcggcctggt ccgttcctac ggcaaccgct actaccgcaa gccgatcatc accggggagc
     2281 tgcggtggac cgccccgatg acggtggaga tgtggcggtt cgcgcagggg ctgaccgagc
     2341 ggccggtgaa gggcatgctc accggcccgt acacgatggt ggagtggtcc ttcgacgagc
     2401 actacccgtc ccgccgcgat gccgtcctgg acatggcgca tgtggtccgc gacgaggcca
     2461 tcgccctggc gatggccggg gcgcgctaca tccaggtcga cgagccggcg atccacaccc
     2521 gtcccgacga ggacttcgac ctggccgtcg aggcgctcga ggtggtcgtg cacggcgtgg
     2581 acgctgagtt ccacacccac gtctgctacg gggacgtcga gctgatctac ccggccatgc
     2641 tccggctgcc ggtgagccag atcagcctgg cgttcaagaa caccgacttc gcgtacttcg
     2701 acctgatgcg caaggacccg tgggagggcg cggacgtggg gctcggggtc ctcgactcgc
     2761 acgaccaccg cacggagacc gtggaggagg tcgtcgaggg gatccgcggc gcgctggagc
     2821 tgctgccggc ggagcggatc tgggtcgagc ccgactgcgg gctgaagacc cggacggtcg
     2881 aggagacccg ggacaagctc cgggtgatgg ccgaggccgt ccggcggctg aagggcgagt
     2941 cgtaagcgcc ggtcccgggc ccggcacatc gcgggcggcc ccggcgggcc ggtatcctcc
     3001 gcgcatgggg gacccctccg agccgcggga gcgcggggga tggcggcacg tgccggccct
     3061 ggtggtctcg ctgctcttcg tgatcccgct ggtcgtcgcc gtggccgggt cgctgcgcga
     3121 cgccggcctg cccccgcccc ggaccgtgga ggtcatcccc gagcgcctcg ccgtcgagaa
     3181 ctacccggag gcctacgcgc tggtggacct gccgcggcag gtcgtgaact ccctgatcgt
     3241 ggtggcgttc gccgtcccgc tgacggtcct ggtggcctcc tgggcggggt tcgcgatctc
     3301 tcggatgcgc cgccgcgccg cgaccgtcgt cgtcgcactg tccctcctgg cgctcgtggt
     3361 cccggccacc gcgctgctgg tcggccgctt cgcggtcttc cggacgctgc ggctgaccga
     3421 cacgttcgtg ccgctcgtcg cgccggcgct gatcgggacc tcgccgttct acgtcctgct
     3481 gtacgcgtgg acgttccggc gactgccggc ggatctgttc gaggccgcca ggctggaggg
     3541 gtcgacgccg ttcgagcagt ggcggcgcgt cggcatgccg ctcgcgaagc cggtgacggc
     3601 ggccgtggcc gccctggcgt tcgccctgac ctgggggaac ctcctcgacc cgctgatcta
     3661 cctgttcgat ccctcgaagt acacggtgcc gctggggctg cgagcgctct cgggggtcga
     3721 tgcgcctgac ctccccctga
//
LOCUS       DASPWO010000313         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_388162, whole genome shotgun sequence.
ACCESSION   DASPWO010000313 DASPWO010000000
VERSION     DASPWO010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388162"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /gene="aspS"
                     /locus_tag="VEO00_07255"
     CDS             <1..1227
                     /gene="aspS"
                     /locus_tag="VEO00_07255"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HYU57830.1"
                     /translation="RDFLVPSRLQRGRFYALPQSPQLFKQLLMVAGLERYYQIVRCFR
                     DEDLRADRHWEFTQLDVEMTFPSEEDVYDLIESMFARLFADVLDVRIPTPFPRLPYDE
                     AMARFGSDKPDTRYGMELADLTETFRGSGFRAFAETVAAGGMVKGFAARGAAGWPRRD
                     LDGLVQEAKARGAAGLVWIALEGEAARSPVEKHLSPDEVAAVRATTGANAGDLALLVA
                     DVPGRVAVALDGLRRLMAERLGLVPAGAWNFLWVTEMPLFEWGEEEGKWVAMHHPFTS
                     PASEDLDPATARARAYDVVLNGYELGGGSIRIHRPDVQRAVFELLGLSRQEAEAKFGF
                     LLEAFRYGVPPHGGIALGMDRVVMLMTGRENIRDVIAFPKTGAGLDLLTGAPAEASEE
                     QIRELGIALRGVPRRG"
     gene            complement(1460..2731)
                     /locus_tag="VEO00_07260"
     CDS             complement(1460..2731)
                     /locus_tag="VEO00_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HYU57831.1"
                     /translation="MTREPLAARMRPRSFDEVVGQRHLFGPGAPLTALAGTGRLPSLV
                     LWGPAGSGKTTLAYLLAASVGGEVVQLSAVSSGVADARKVLERARGSLVPTVLFIDEV
                     HRWSKAQQDVLLPAVEDGTVTFIGATTENPYFSLVTPLLSRTVLLRLEPLAADDVLAL
                     LERALADAERGLGEERVKVAHDALEHLVEVAGGDARVALTGLEAAVLAARAAGRDAVD
                     LPLAEEAIQQKAVVYDRQGDAHYDVISAFIKSLRGSDPDAALFWLARMLEAGEDARFI
                     ARRMVILASEDVGLADPRALLVASAAAHAVEYVGLPEAQLNLAEAAIYLARAPKSNSV
                     IAALGRAREDAASADPVPRHLRDAHYPGARRLGHGKGYRYPHDYPGHEVEQEYRPVRF
                     EGRRYYEPSGQGEETGDGSAAPPAREDGTSR"
BASE COUNT          364 a          992 c         1051 g          377 t
ORIGIN      
        1 cgcgacttcc tggtcccctc gcgcctgcag cgggggcggt tctacgcgct gccgcagtcg
       61 ccacagctgt tcaagcagct gctgatggtc gccgggctgg agcgctacta ccagatcgtg
      121 cgctgcttcc gcgacgagga cctgcgggcc gaccgacact gggagttcac acagctcgac
      181 gtcgagatga cgttcccctc cgaggaggac gtctacgacc tgatcgagtc gatgttcgcc
      241 cgcctgttcg ccgacgtcct cgacgtgcgg atcccgacgc ccttcccgcg cctgccgtac
      301 gacgaggcga tggcccgctt cggctccgac aagcccgaca cgcgctacgg gatggagctc
      361 gccgacctga cggagacctt ccgggggagc ggcttccgcg cgttcgccga gacggtcgcc
      421 gccgggggga tggtcaaggg gttcgcggcg aggggagcgg ccggctggcc gcgccgcgac
      481 ctcgacgggc tggtgcagga ggccaaggcc cggggcgccg ccgggctggt ctggatcgcg
      541 ctcgaggggg aggcggcccg ctcgccggtc gagaagcacc tctcgcccga cgaggtggcc
      601 gcggtccggg cgacgaccgg ggcgaacgcc ggcgacctgg cgctgctcgt cgccgacgtt
      661 ccgggacggg tggccgtcgc gctcgacggg ctgcgccggc tgatggccga gcgcctcggc
      721 ctggtccccg ccggcgcctg gaacttcctg tgggtgaccg agatgccgct gttcgagtgg
      781 ggcgaggagg aggggaagtg ggtggcgatg caccacccgt tcacctcgcc ggcctcggag
      841 gacctcgacc cggcgaccgc ccgggcgcgc gcctacgacg tggtgctgaa cgggtacgag
      901 ctggggggcg ggtcgatccg gatccatcgc cccgacgtgc agcgggcggt gttcgagctg
      961 ctcggcctct cacggcagga ggcggaggcc aagttcgggt tcctgctcga ggcgttccgc
     1021 tacggcgtgc caccccacgg cgggatcgcc ctgggcatgg accgcgtggt gatgctcatg
     1081 accgggcgcg agaacatccg cgacgtgatc gccttcccga agaccggcgc cggcctcgac
     1141 ctgctgaccg gcgcccccgc cgaggcgagc gaggagcaga tccgcgagct cggcatcgcc
     1201 ctgcgcggcg tgccgcgccg cggttgatcg cgtccggtcc ccgggataaa gtcgagtcgg
     1261 cggcgtaccg cctggatcgc ggattcgttg taccagctca tcggatccgg gagcccggcg
     1321 ccggtcgtgg tgcagcaccc cgaagcgggg ttctctgaag cggccgggag ggcacccacc
     1381 tgcgtgtgca gaaggttccg aagatgcggt ccaggggtac gccgccctcg tcgtcgagca
     1441 cggtcgtacg ggaacgggac tagcgcgacg tgccgtcctc acgagcgggc ggcgctgcgc
     1501 tcccgtctcc ggtctcctcg ccctggccgc tgggctcgta gtaacggcgg ccctcgaagc
     1561 ggaccgggcg atactcctgc tcgacctcgt gcccggggta gtcgtgcggg taccggtagc
     1621 ccttcccgtg gccgaggcgc ctcgcgccgg ggtagtgcgc gtcgcgcagg tggcgcggca
     1681 cggggtcggc cgacgcggcg tcctcgcggg cccggccgag cgcggcgatc acgctgttcg
     1741 acttcggcgc gcgggccagg tagatcgccg cctcggccag gttcagctgc gcctcgggca
     1801 ggcccacgta ctcgaccgcg tgggcggcgg cggaggccac gagcagcgcc cgggggtcgg
     1861 ccaggccgac gtcctccgac gccaggatga ccatccgccg ggcgatgaac cgtgcgtcct
     1921 cgccggcctc cagcatgcgg gccagccaga agagcgcggc gtccgggtcg gagccgcgca
     1981 ggctcttgat gaacgccgag atcacgtcgt agtgcgcgtc gccctgccgg tcgtagacga
     2041 cggccttctg ctggatggct tcctcggcca gcggaaggtc caccgcatcc cgtccggccg
     2101 cccgcgccgc caggaccgcc gcctcgagcc ccgtcagcgc gacccgggcg tcgccgcccg
     2161 cgacctcgac caggtgctcg agcgcgtcgt gggcgacctt cactcgctcc tcgccgagcc
     2221 cgcgctccgc gtcggcgagc gcacgttcga gcagggcgag gacgtcgtcc gccgcgagcg
     2281 gctccagacg caacagcacc gtgcgcgaca ggagcggggt gacgagcgag aagtacgggt
     2341 tctccgtggt ggcgccgatg aacgtgacgg tcccgtcctc caccgccggg agcaacacgt
     2401 cctgctgggc cttgctccag cggtggacct cgtcgatgaa caggacggtg gggacgagcg
     2461 agccacgggc ccgctccagc accttgcggg cgtccgccac gccggaggac accgccgaga
     2521 gctggacgac ctccccgccg acggatgcgg ccagcagata ggccagcgtg gtcttgccgc
     2581 tcccggcagg cccccacagc acgagtgagg gcaggcggcc ggtgccggcc agcgcggtga
     2641 gcggggcgcc ggggccgaac aggtggcgct gcccgacgac ctcgtcgaac gaacgcgggc
     2701 gcatccgcgc cgccagcggc tccctggtca ctgacgcgca gcgtaccgga gcgcgtggat
     2761 ttggcctcgg ggggcgcacg gggg
//
LOCUS       DASPWO010000314         3897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_978591, whole genome shotgun sequence.
ACCESSION   DASPWO010000314 DASPWO010000000
VERSION     DASPWO010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978591"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1480
                     /gene="hppD"
                     /locus_tag="VEO00_07265"
     CDS             341..1480
                     /gene="hppD"
                     /locus_tag="VEO00_07265"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HYU57832.1"
                     /translation="MSQRYESGSAEEAATQHDEMPLIGIDHVEYHVGNARQAAYYYTR
                     AMGFGVTAYGGPETKVRDRASWVLEQGEARLVFTGSMGPEHPIARHVLAHGDGVHDVA
                     FRVPDAAHAYAYATAHGATGVLEPTVFEDEHGKVVRSTIAAYGDTVHSFVERENYSGP
                     FLPGYRAEEPIPDEGPPVGITQIDHVVCNVELGKMNEWAAFYANVLGFAQLVHFRDDQ
                     ISSEYTALMSKVMWDGSGRVKLPINEPAPGKKKSQIEEYLEFYRGPGVQHIAMATDDI
                     VGAVDALRRRGMRFIRVPATYYEAAREKVGEIEEAWADIERLNVLADRDEEGYLLQIF
                     TKNVQDRPTVFYEVIERRGSRGFGLGNFKELFLAIEREQEERGNL"
     gene            complement(1554..3377)
                     /locus_tag="VEO00_07270"
     CDS             complement(1554..3377)
                     /locus_tag="VEO00_07270"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYU57833.1"
                     /translation="MRRAISLVLSALLLVAIAGLSTSADADSSAGKAKFAPSSVVSDG
                     KNTSTAEPVAPAALPSTNTNGCSDNATNNVRVNQECTNHTELDLLGRGQAQNETAIAV
                     SPTDPNNLIAGQNDYRRGDGVCGFAYSYDGGQTWGDGLLPISFVAPGFTAPRHYWDAG
                     GDPAVAFDADGNAYYACLAFNRGGTSDTDSDASGIFLFTSTDGGASWNMNPTPVAISP
                     GDGSDGVGLHDKEYIAVDKSPASPYQGRVYIAWVKYSLDFTAAVINLAWSDNAGASWH
                     QSGNVFSDSVLCPINFNGAPAGKCNANQFPVPFTAPNGDVYVVSQNFNNNTFYGPGDN
                     HNQILLAKSTDGGSHFGPPVKVADWYDLPDCFTYTGDDAGRACVPTAPLSDRSVFRAT
                     NYPSAVAVSTTRVVVDFGSYINQHSNSTKGNCVPTGFSQFGLNTYDGVGVTNGCNNDI
                     VRSISNDGGASFTGTDTNVEKLTVISNEMVGKPLADQWWQWTAFNPATNHVVTSYYDR
                     KYGQSQQNGNMDITMHRGDNSYVRVTNKSIPPSRDFTGASGSSLFLGDYAGLAVGTDG
                     QAHPFWTDTRNAAFAPNPANPLVLSFSHFSADVYTRRLLGL"
     gene            complement(3484..>3897)
                     /locus_tag="VEO00_07275"
     CDS             complement(3484..>3897)
                     /locus_tag="VEO00_07275"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYU57834.1"
                     /translation="RTLFVDPRFTTRVAQLSRALGPGARVEDMAEYFRHLQGLDPLVM
                     LKMAEAMRAHSARDVLPTVKPPALIIAAELDTFTPLALAEHMRRAIPHSEFHLVRGAA
                     HGAVIERPAEIDGAVQDFLRRHLKPRAAKAASSRR"
BASE COUNT          589 a         1261 c         1430 g          617 t
ORIGIN      
        1 acgcaggtcg atccgccgcg gctgatcagc aggttcagga tccggacccg ggtcgggtcg
       61 gccagggcct tgaaccgccg ggcgatctcc tcggcctccc cggggagcag gcccggctgc
      121 ccgatcgggc agcagggggc ggtgcggtcg atcacctcca gctcggtgcg catgagcgga
      181 gcataacggc tgtatcgaaa tctgtcaatg gaagggagta accgaggaac gcgttgcgga
      241 cgtcgaggtg gccgtggagc tgccggcggc ggcgtcgagg gaccgcgtcc tctccgggta
      301 gactggcacg gcgggacgac gccagccgga ggtgacgacg atgtcgcagc ggtacgagag
      361 cggatcggcc gaagaggcgg cgacgcagca cgacgagatg ccactcatcg gcatcgacca
      421 cgtcgagtac cacgtgggaa acgcccgtca ggccgcctac tactacacgc gggcgatggg
      481 gttcggcgtc accgcgtacg gcgggcccga gacgaaggtc cgggaccgcg cttcgtgggt
      541 gctcgaacag ggcgaggccc ggctggtgtt caccggctca atggggccgg agcacccgat
      601 cgcgcggcac gtgctggcgc acggcgacgg cgtccacgac gtggcgttcc gcgtccccga
      661 cgccgcgcac gcctacgcgt acgccaccgc tcacggcgcc accggtgtgc tggagccgac
      721 ggtcttcgag gacgagcacg gcaaggtcgt ccgatcgacg atcgccgcct acggcgacac
      781 cgtgcactcg ttcgtcgagc gcgagaacta ctccggcccg ttcctgcccg gctatcgggc
      841 cgaggagccg atccccgacg agggcccgcc ggtcgggatc acgcagatcg accacgtcgt
      901 ctgcaacgtc gagctcggga agatgaacga gtgggcggcg ttctacgcga acgtgctcgg
      961 gttcgcccag ctggtgcact tccgcgacga ccagatctcg agcgagtaca ccgctctgat
     1021 gtccaaggtg atgtgggacg gctccgggcg ggtgaagcta ccgatcaacg agccggcccc
     1081 cggcaagaag aagagccaga tcgaggagta cctggagttc tatcgcgggc ccggggtgca
     1141 gcacatcgcg atggctaccg acgacatcgt cggcgcggtg gacgccctgc gccggcgcgg
     1201 gatgcgcttc atccgcgtgc cggccacgta ctacgaggca gcccgggaga aggtcgggga
     1261 gatcgaggag gcctgggcgg acatcgagcg gttgaacgtc ctggccgacc gggacgagga
     1321 gggctacctg ctgcagatct tcaccaagaa cgtccaggac cgcccgacgg tcttctacga
     1381 ggtcatcgag cggcgaggct cacgaggctt cggcctcggc aacttcaagg agctcttcct
     1441 ggccatcgag cgcgagcagg aggagcgggg caacctctga gcgcgcgtct tgacctgcac
     1501 gcggaagggg ccgggcggat cgcccggccc cttcgcgtct cggtcttccc ccgtcagagc
     1561 cccagcaggc gccgcgtata cacgtcggcg ctgaagtggg agaacgagag gaccagcggg
     1621 ttcgccgggt tcggggcgaa cgccgcgttg cgcgtgtcgg tccagaacgg gtgggcctgt
     1681 ccgtcggtcc cgacggccag gccggcgtag tcgcccagga acagcgagct cccgctcgcc
     1741 cccgtgaagt cgcgcgaggg cgggatgctc ttgttcgtca cgcgcacgta gctgttgtcg
     1801 ccgcggtgca tcgtgatgtc catgttcccg ttctgctgcg actgcccgta cttgcggtcg
     1861 tagtagctgg tgacgacgtg gttcgtggcc gggttgaagg ccgtccactg ccaccactgg
     1921 tccgccagcg gcttgccgac catctcgttg ctgatcacgg tcagcttctc gacattggtg
     1981 tcggtcccgg tgaaggacgc gccgccgtcg ttcgagatgc tgcggacgat gtcgttgttg
     2041 cacccgttgg tgacgcccac gccgtcgtac gtgttcagcc cgaactgcga gaacccggtc
     2101 gggacgcagt tccccttggt cgagttggag tgctggttga tgtaggagcc gaagtcgacg
     2161 acgacccgcg tggtgctgac cgcaacggcc gacgggtagt tggtcgcccg gaacacgctg
     2221 cggtccgaga gcggcgccgt cgggacgcag gcccgcccgg cgtcgtcacc ggtgtaggtg
     2281 aagcagtccg ggaggtcgta ccagtcggcg accttgaccg gcggcccgaa gtgggagccg
     2341 ccgtcggtcg acttcgccag caggatctgg ttgtggttgt cgcccggccc gtagaacgtg
     2401 ttgttgttga agttctggga caccacgtag acgtcgccgt tcggcgccgt gaacggcacc
     2461 gggaactggt tggcgttgca cttccccgcc ggcgcgccgt tgaagttgat cgggcagagc
     2521 accgagtccg agaagacgtt cccgctctgg tgccagctgg cgccggcgtt gtccgaccag
     2581 gccaggttga tcacggcggc cgtgaagtcg aggctgtact tgacccacgc gatgtagacg
     2641 cgcccctggt agggggaggc ggggctcttg tcgaccgcga tgtactcctt gtcgtgcagc
     2701 cccacgccgt ccgacccgtc accggggctg atcgccaccg gggtcgggtt catgttccag
     2761 gacgcgccgc cgtcggtcga ggtgaacagg aagatcccgc tggcatcgct gtcggtgtcg
     2821 ctcgtcccgc cgcgattgaa ggccaggcac gcgtagtagg cgttcccgtc ggcgtcgaag
     2881 gccacggccg ggtcgccgcc ggcgtcccag tagtggcgcg gggcggtgaa gcccggcgcc
     2941 acgaagctga tcggcaggag accgtcgccc caggtctggc cgccgtcgta cgaataggcg
     3001 aacccgcaca cgccatcgcc gcgccggtag tcgttctgcc cggcgatcag gttgttcggg
     3061 tcggtcgggc tcacggcgat ggccgtctcg ttctgcgcct ggccccggcc gagcaggtcg
     3121 agctcggtgt ggttggtgca ctcctgattg acccggacgt tgttcgtggc gttgtccgag
     3181 cacccgttcg tgttggtgga ggggagcgcg gcgggcgcga ccggctccgc agtgctcgtg
     3241 ttcttcccgt cggagaccac gctcgacggg gcgaacttgg ccttcccggc cgagctgtcg
     3301 gcgtccgccg acgtggagag cccggcgatc gcgaccagca gcagggcgct gagcaccagg
     3361 gagatggctc gtctcattgg tcaatcctcc tcacggagat gggcgggccg tgggcccgat
     3421 cggggcattc aaaccctcgc cgggagggcg agtcaaggat gcgagcgagg tggtcgacgc
     3481 gcgtcatctc cgcgaggagg ccgccttggc ggcacgcggc ttcagatggc ggcgcaggaa
     3541 gtcctgcacc gccccgtcga tctcggccgg cctctcgatc acggcgccgt gcgcggcccc
     3601 gcgaaccagg tggaactccg agtgggggat ggcccggcgc atgtgctcgg ccagggcgag
     3661 cggggtgaac gtgtccagct cggcggcgat gatcagggcc ggcggcttca cggtcggcag
     3721 cacgtcgcgg gccgagtggg cccgcatggc ctccgccatc ttcagcatca ccagcgggtc
     3781 gagcccctgc aggtgccgga agtactcggc catgtcctcg acgcgcgcgc ccgggccgag
     3841 ggcgcgggac agctgcgcca cccgggtggt gaaccgcggg tcgacgaaca gcgtccg
//
LOCUS       DASPWO010000315         4592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_304491, whole genome shotgun sequence.
ACCESSION   DASPWO010000315 DASPWO010000000
VERSION     DASPWO010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304491"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VEO00_07280"
     CDS             complement(<1..195)
                     /locus_tag="VEO00_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HYU57835.1"
                     /translation="MSARVELVNYGEDAPLYDHGPQHPLRPARVVLTLGLIRDFGLVD
                     GELVREVRGRDATEEELLLVH"
     gene            complement(192..3164)
                     /locus_tag="VEO00_07285"
     CDS             complement(192..3164)
                     /locus_tag="VEO00_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYU57836.1"
                     /translation="MSRGLAALGIRPGETATALRLLALMVVLFVGFTLGSNAVESLLL
                     ARVGANVLPYLYVALGPVTCVVVSSASGVLARGRSALGPVFLAVAGVVAAARALLLVG
                     GDAVYAALWIVMMILWTIAVFNAWAVAGVVHDARQAKRLYPLYGAGIITGIVLGGAIT
                     GPLASSVGTENLFVPWGVATVAAWWLTRSVLSAGPPAAAAVRERRGGPGLRAGYRAVR
                     ATPLLRWMAVSLALLALLYFAVSFVFARAVAARYPRAAELAGFLGIFIGTASGVALLV
                     SLLVANRLFARFGVATMVLVLPALYALGFAVLAVSVAFLAVAAFRFGQIVWVNGVWGS
                     GWQALFNVVPAGRRAAVRSFMDAFPLQAGIVAAGGLLILAERGLTPRQVSVAGAAGAL
                     VTLVAVARARRAYGEALAEALREGNPDVFAARERAVGGALSDGAAIAALASGAADPDP
                     GVRRLALELLADAGAAEAPAALARGAADPEPQVRETSVRALARMDAAVAREVLARALA
                     DPVAAVRAAAARAAVGAAGEATGEAVAEALRGALRDPEPEVRFAAAAALAATGDREAR
                     AVLLASGGAEDPVERAAGLNGLAVAMGPSVSVGELAREIPVIVPALGDPEGRVRRAAV
                     AALAAAGSSAIGPVGRALEDPRLEDGALEALLGLFDADPDLLRRYAEGRSRRALRYVT
                     LRRGTAADGDERLALLHDALDERARADGTRALRALTAGADSETITLALENLESGHPDQ
                     RANALEILEAAAEGTLVRPLLVVWEPPPSTGQVVTTLARVLEDHEEDPWVRACAALAA
                     GALDDEALTGVLDRVAGSDSDPLVRETAAAARRGNAGMETLATLSLMERVLFLRKVPL
                     FAGLAPSELRAVAEVCTEQVFGAGAALARQGEEGEELHIVVSGAIRVTVAAGGAEHEV
                     AVRRAGEYVGEMAIVSRAPRMASLAAVGDVRVLSIDRRRFERILRDRPEVGLAVMRVL
                     ADRLRESDAARGPAEGAG"
     gene            complement(3161..3862)
                     /locus_tag="VEO00_07290"
     CDS             complement(3161..3862)
                     /locus_tag="VEO00_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57837.1"
                     /translation="MERAYEEGRRIAAPLGDAATEGEALLGLAFAAAMREDAAARDDF
                     LARSRERYEAADDPVGLGRVIMAHAFLGPRERVDTAQFRRLAGEAAGLLLRGGDRVGW
                     VETMQGLALTAAIDGDLAAAREIMRNTLDHGLRSLNRAALVVTTEGVAFTANLAGEHD
                     RAARLAGAIDRLAEVLGVVVPHPLRVITHVRETAREALGDETFRIRVEEGRAMTFERA
                     VGYARESLEALTSGR"
     gene            4412..>4592
                     /locus_tag="VEO00_07295"
     CDS             4412..>4592
                     /locus_tag="VEO00_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57838.1"
                     /translation="MSADRSTNTTPSRNAETNPRAASTARRVFPVPPGPVRVTRRDDP
                     TRSRTAWSSAARPTND"
BASE COUNT          656 a         1847 c         1621 g          468 t
ORIGIN      
        1 gtgaaccagc agcagttcct cctcggtcgc gtcgcggcca cgcacctccc tcacgagctc
       61 cccgtccacc agaccgaagt cgcggatcag gcccagggtg agaacgaccc gggccggccg
      121 cagcggatgc tgcgggccgt ggtcgtagag cggcgcgtcc tccccgtagt tcaccagctc
      181 gacccgtgcg ctcatccggc tccttcggcc gggccgcggg cggcgtcgga ctcgcgcagg
      241 cggtcggcca gcacccgcat cacggccagg ccgacctccg gacggtcgcg caggatccgc
      301 tcgaagcggc gccggtcgat cgagagcacc cgcacgtcgc cgaccgctgc gagcgaggcc
      361 atccgcgggg cgcggctgac gatcgccatc tcgccgacgt actcgccggc ccggcgcacc
      421 gccacctcgt gctccgcccc gccggcggcc acggtcaccc ggatcgcccc agagaccacg
      481 atgtgcagct cctcgccctc ctcaccctgc cgcgcgaggg ccgcgccggc cccgaagacc
      541 tgttcggtgc agacctcggc caccgcccgg agctcggacg gcgcgaggcc ggcgaagagg
      601 ggaaccttcc gcaggaacag cacccgctcc atcagcgaga gcgtcgccag ggtctccata
      661 cccgcgttcc ccctgcgagc cgccgccgcg gtctcacgga cgagcggatc actgtcggag
      721 cctgccacac gatcgaggac gccggtgagc gcctcgtcgt cgagcgcgcc ggccgcgagc
      781 gccgcgcagg cccgaaccca tgggtcctcc tcgtggtcct cgaggacccg cgccagcgtg
      841 gtcaccacct gaccggtcga cggaggcggc tcccacacga ccaggagcgg gcggacgagg
      901 gtcccttccg ccgccgcctc caggatctcc agggcgttag cgcgctggtc cggatggccg
      961 ctctcgaggt tctccagggc cagggtgatg gtctcggaat ccgcgccggc cgtcagggcg
     1021 cggagcgcgc gcgtgccgtc cgcccgagcg cgctcgtcga gcgcgtcgtg caggagcgcc
     1081 agccgctcgt cgccgtccgc cgccgtcccg cggcgcaggg tgacgtaccg cagcgcgcgc
     1141 cgggaccgcc cctcggcgta gcggcggagg aggtccggat cggcgtcgaa cagcccgagc
     1201 agggcctcga gggcgccgtc ctccagacgg gggtcctcga gcgcgcgccc caccgggccg
     1261 atggccgagg agcccgcggc cgcgagtgcc gccacagccg ctcgacgcac ccgtccctcc
     1321 ggatcgccga gggctgggac gatcacgggg atctcgcggg ccagctcccc cacggacacc
     1381 gacggcccca tcgccacggc cagaccgttc aggccggcgg cgcgctccac ggggtcctcc
     1441 gccccccccg atgccagcag caccgcgcgg gcctcccggt cccccgtggc cgccagcgcg
     1501 gccgcggcgg cgaagcggac ctccggctcc gggtcgcgca gagccccgcg gagcgcctcc
     1561 gccactgcct cgcccgtcgc ctcgcccgcc gccccgacgg cggcacgggc tgcggcggcg
     1621 cggaccgcgg cgaccggatc ggccagcgcg cgggcgagga cctccctcgc caccgccgcg
     1681 tccatccggg caagggcgcg aacggaggtc tcgcggacct ggggctccgg gtcggcggcg
     1741 ccgcgggcga gcgccgccgg ggcctctgcc gctccggcgt ccgcgaggag ttcgagcgcc
     1801 aggcggcgta cgcccggatc gggatccgcc gctcccgagg cgagggcggc gatggccgcc
     1861 ccgtcgctca gggcgccgcc gaccgcacgc tcccgggcgg cgaacacgtc cgggttcccc
     1921 tcccgaagcg cctccgccag cgcctccccg taggctcgcc gggcccgcgc caccgccacc
     1981 agcgtgacga gcgcccccgc ggctcccgcg accgacacct gccgcggcgt gagcccgcgc
     2041 tcggccagga tcaggagccc acccgccgcg acgatccccg cctgcagcgg gaacgcgtcc
     2101 atgaacgacc ggacggcggc ccggcgaccg gcgggcacca cgttgaagag ggcttgccag
     2161 cctgaccccc ataccccgtt gacccagacg atctggccga agcggaaggc cgccaccgcg
     2221 aggaaggcga cgctgaccgc cagcacggcg aagccgagcg cgtacagcgc cggcaggacg
     2281 agcaccatcg tcgcgacgcc gaagcgggcg aacaggcggt tcgccaccag gagcgagacg
     2341 aggagcgcga cgccgctggc ggtcccgatg aagatcccga ggaacccggc cagctcggcc
     2401 gcccgcggat agcgcgccgc caccgcccgc gcgaagacga agctgaccgc gaagtagagc
     2461 agcgcgagca gcgccagcga gacagccatc caccgcagca acggggtggc gcggacggcg
     2521 cgatagccgg cccgcaaccc cggaccgccg cgccgctccc ggaccgcggc cgccgcgggc
     2581 ggccccgcgc tcaggaccga cctggtcagc caccaggcgg cgaccgtcgc caccccccac
     2641 gggacgaaga ggttctcggt accgacgctc gacgcgagcg gcccggtgat ggcgccgccg
     2701 aggacgatgc ccgtgatgat cccggcgccg tacagcgggt agaggcgctt cgcctgccgc
     2761 gcgtcgtgga ccacgcccgc cacggcccag gcgttgaaca cggcgatggt ccagaggatc
     2821 atcatcacga tccacagcgc ggcgtagacg gcgtcgccgc ccaccagcag cagtgcgcgc
     2881 gcggccgcca ccacgccggc gacggcgagg aagaccgggc cgagcgcgga ccggccgcgg
     2941 gcgaggaccc cgctcgccga cgacacgacc acgcatgtca ccggccccaa cgcgacatac
     3001 aggtacggca acacgttcgc gccgacccgc gccagcagca gtgactccac ggcgttcgag
     3061 cccagcgtga acccgacgaa cagcaccacc atcagcgcca gaagccggag tgccgtcgcc
     3121 gtctcacccg gccggatccc gagcgcggcc agtccccggc tcatctcccg ctcgtcaggg
     3181 cctcgagcga ctcgcgcgcg taccccacgg cgcgctcgaa ggtcatcgcg cgcccctcct
     3241 ccactcggat gcggaacgtc tcgtcgccga gcgcttcgcg ggcggtctcc cggacatggg
     3301 ttatgacccg caggggatgg gggacgacga cgccgagcac ctcggccagc cgatcgatcg
     3361 cgccggcgag gcgcgcggcg cggtcgtgct ctccggcaag gttcgcggtg aacgccactc
     3421 cctccgtcgt gaccaccagc gcggcccggt tcaatgaccg caggccatgg tcgagcgtgt
     3481 tccgcatgat ctccctcgcc gcggcgagat cgccgtctat ggccgcggtc aacgccagcc
     3541 cctgcatggt ctccacccag ccgacgcggt caccgccccg caggagcagc cccgcggcct
     3601 cacccgcgag acggcggaac tgggccgtgt cgaccctctc ccgtgggccg aggaacgcat
     3661 gcgccatgat gacccggccg agcccgaccg ggtcgtccgc ggcctcgtag cgttcgcggc
     3721 tccgggccag gaaatcgtcc cgggccgctg cgtcctcgcg cattgccgcg gcgaaggcca
     3781 ggccgagcag cgcctcgccc tcggtggccg cgtcaccgag gggggccgcg atacgccggc
     3841 cctcctcgta ggcgcgttcc atggtccccg aggccctcca ggctgtcgcc cagctcccgg
     3901 tcggggttcg cgaccgcctc cgcgtcgtcg agcctccagc caccggcgaa cacgggcagc
     3961 cgccggaaga ggacgccccc ggctcggcca ggaggtcgtg gccccaggcc atggtctcgc
     4021 ggagcgcgcg cagatctcgg cgacgatcgc cgccagcgag ccgtcgaggg agaaggcggg
     4081 atccacggcg gtcgcccgcg cggcgaacag cgcgacggca tcgctctccg aacccgcgcg
     4141 gggcgcgacg accggcatcg ggggaaccgg gaactcctgc tcgccgacga tcgacagcgg
     4201 cgagcggctg gtggcgagga ccttcaccgc cggcgcctcg gccaggagcc gcgccacgtc
     4261 gcccgcggcc tgcaggacct gttcgacgtt gtcgagcacc agcagcatct cccggggacg
     4321 gaggtgctcg acgaccgcct cgatcggggg tccctccagc gaccgcaggc cgatcgccgc
     4381 ggcgatcgcg ggggggacca gccgggcgtc ggtgagcgcc gacaggtcga cgaacacgac
     4441 gccgtcgcga aacgcggaga ccaatccgcg ggcggcctcc acggccaggc gcgtcttccc
     4501 ggtcccgccg gggccggtca gggtgacgag gcgggacgac ccgacccgct cgcgtaccgc
     4561 ctggagctct gccgcccgcc cgacgaacga cg
//
LOCUS       DASPWO010000316         2456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1148461, whole genome shotgun sequence.
ACCESSION   DASPWO010000316 DASPWO010000000
VERSION     DASPWO010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148461"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1073)
                     /locus_tag="VEO00_07300"
     CDS             complement(255..1073)
                     /locus_tag="VEO00_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57839.1"
                     /translation="MSARLRAMTDRELGRALVDAGAHLAVPPLPDLAPAVARAVVAAP
                     ERRGARGRTALATLRDLVRPPGASSRRRALALAVALLVLLAGAAIATGLAVRGVRLLF
                     REEPVPTSAAPIGSRLGLGERVSLPEAGAGVAFDLVVPTLGPPDEVWLSDAATGGLVT
                     FVYAPSDRLPELGRSGVGMLLSQFAGRTDAPSIEKEILSGTEVVEVEVNGEPGYWITG
                     QPHAVRFLDSEGREVPPGPRLAGDTLIWQRGPLTLRMEVTLPLEAALGLAESVA"
     gene            complement(1070..1654)
                     /locus_tag="VEO00_07305"
     CDS             complement(1070..1654)
                     /locus_tag="VEO00_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU57840.1"
                     /translation="MEGRPLVPTEDESELIERARGGEVGAYEELVRRYQGIAGRTASF
                     VLGTSGEVEDAVQEGFVKAYRALDRFRPGAPFRPWLLRIVANEARNRRKAAGRRAALV
                     LRAAQDRRSGDAAPSPESAVVDDEERRALVAALNELREDDRLVLAYRYFLELSEEEMA
                     VALGVARGTVKSRLSRAMRRLRGAVTAGTEGTER"
     gene            complement(1784..2299)
                     /locus_tag="VEO00_07310"
     CDS             complement(1784..2299)
                     /locus_tag="VEO00_07310"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017556948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HYU57841.1"
                     /translation="MVLGDITEQEVDAVVNAANSSLLGGGGVDGAIHRRGGPEILAEC
                     RRLRAERFPDGLPTGQAVATSGGRLPARWVIHTVGPVYAMSEDRSALLASCHVESLRI
                     ADELGARTVAFPAISTGVYGYPLEEAAPVALRAVLEADTAVEEARFVLFDRRARDAFR
                     RAVDTPGGPER"
BASE COUNT          315 a          992 c          867 g          282 t
ORIGIN      
        1 gaagcagctc ccggccagca cgacccggtc gccttcctgg tcggtcggga cggcgttgtg
       61 acagccgccg ccggccagcg cggacggcgt cgccagcagc acggtcgcgg cgagcgttgc
      121 cgcggccgcg agcgcgagag cgatccgtcg attcatgagc ctgcctcctt cgccggacac
      181 ggtccggcct ccgcccctcc tacaaccggg ggccgccgtg ggttcctcgc cgatcgggcc
      241 ggggcgcggt tgcctcaggc caccgactcc gcgaggccga gcgcggcctc cagcggcagg
      301 gtcacctcca tgcggagggt gagcggcccg cgctgccaga tcagggtgtc gcccgccagg
      361 cgcggcccgg gcgggacctc ccggccctcg ctgtccagga accgcacggc gtgcggctgg
      421 ccggtgatcc agtagccggg ctcgccgttc acctcgacct cgaccacctc ggtcccggac
      481 aggatctcct tctcgatcga cggggcatcc gtgcggccgg cgaactgcga gagcagcatt
      541 cccacgcccg accgtccgag ctcgggcagt cggtccgagg gagcgtagac gaacgtcacc
      601 aggccgccgg tcgccgcgtc cgagagccac acctcgtccg gcggccccag cgtgggcacg
      661 accaggtcga acgccacgcc cgcgcccgcc tccggcagcg agacccgctc gccgaggccg
      721 agccgtgacc cgatcggcgc cgcgctcgtc ggcaccggtt cttcgcggaa aagtagccga
      781 acgccgcgca ccgcgagccc ggtggcgatc gcggcgccag ccagcagcac cagcagcgcc
      841 acggcgagcg ccagggcgcg ccgccggctg gaggcccccg gcggccggac caggtcgcgc
      901 agcgtggcga gcgccgtccg cccccgcgca ccgcggcgct ccggcgcggc gaccaccgcc
      961 cgggccaccg ccggcgcgag gtcggggagc ggcgggacgg ccaggtgcgc tccggcgtcg
     1021 acgagcgcgc gcccgagctc ccggtcggtc atcgcgcgca gacgggcgct catcgttccg
     1081 ttccctccgt gccggccgtg acggccccgc gcaggcggcg catcgcccgg gaaagccgag
     1141 acttcacggt cccgcgggcg acccccagcg ctacggccat ctcctcctcc gagagttcca
     1201 ggaagtagcg ataggcgagc accagccgat cgtcctcgcg cagctcgttc agggccgcga
     1261 ccagcgcccg ccgctcctcg tcgtccacga ccgccgactc gggggatggg gccgcatccc
     1321 ccgagcggcg gtcctgcgcc gcccgcagca ccagtgcggc gcgacggccg gccgccttgc
     1381 gccggttgcg cgcctcgttc gccacgatcc gcagcagcca gggacggaac ggcgcgcccg
     1441 ggcggaagcg gtccagggcc ctgtaggcct tcacgaaccc ctcctgcacc gcgtcctcga
     1501 cctccccgct cgtcccgagc acgaacgacg cggtccgccc ggcgatcccc tgataccggc
     1561 gaaccagctc ctcgtacgcg ccgacctcgc cgccgcgcgc gcgctcgatg agctcggact
     1621 cgtcctccgt gggtaccagt ggtcggccct ccactgcgcg gtcgctccgc tcgtccgcct
     1681 ccagtaccgc gcaacccggc cggcggttcc cggctcagcc cgccgtccct cccgggggga
     1741 tcgctcgccg ggcgtggccc agacgagcgc cgcccggggc gagctatctc tccggacccc
     1801 ccggcgtgtc gacggcccgg cgaaaggcgt cccgcgcgcg gcggtcgaac aggacgaacc
     1861 gcgcctcctc gacggcggtg tcggcctcga gcacggcgcg cagcgccacg ggggcggcct
     1921 cctcgagcgg gtagccgtac acgccggtcg agatggcggg gaaggccacc gtccgcgcgc
     1981 ccagctcgtc ggcgatgcgc agcgactcga cgtggcagga ggcgagcagg gccgagcggt
     2041 cctccgacat cgcgtagacg ggtccgacgg tgtggatcac ccagcgggcc gggagtcgcc
     2101 ccccgctggt ggccaccgcc tggcccgtgg gcagcccgtc ggggaaccgc tcggcccgaa
     2161 ggcgccggca ctccgccagg atctcggggc cgccgcggcg gtggatcgcc ccgtccacgc
     2221 cgcccccgcc gagcagcgac gagttcgcgg cgttcaccac cgcgtccacc tcctgctcgg
     2281 tgatgtctcc cagcaccaac gttacccgag gagccaacgc cggaacgata gactccgccg
     2341 gatgcgcatc gccagggagg tccgccgctt catcgagcgc gagtgcctgg gcggggtgac
     2401 cgaggacgac ccgctcgcct ccgggctcct cgactctctg gccatgggcc agctga
//
LOCUS       DASPWO010000317         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1232956, whole genome shotgun sequence.
ACCESSION   DASPWO010000317 DASPWO010000000
VERSION     DASPWO010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232956"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VEO00_07315"
     CDS             complement(<1..1178)
                     /locus_tag="VEO00_07315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYU57842.1"
                     /translation="MRTEQPPTVLAGRYALEQELGTSGTGTSWSATDTVLDRRVVVKL
                     ISPELARDPAFAERLDLELRMVALASHRGLARLLDTGKDDGISFIVREYFEGDSARAV
                     IARDGNVPVDQGAGLLRGALHAIAAAHEAGVLHLDLKPENVFECMDGTIRVCDLGFGE
                     AVVRSMPPHEAIEILQPAPIAPETAAGAAPDVRTDVWAAGALLYELLTGRTPAAGDAS
                     PRDHRPNVTRKLDDVVRKALSTEPDDRFATAVEFGAALDDVAGVATERAAATARRSGL
                     EETAAVAPAPSPPRFSTFRTWLAVPLLVVVIAASALGAGLWLGKLELGGPVGIRFGPA
                     EESPAPNTRVVGFISVAPMDPYGDGQENDSGLRFVADDDPLTVWKSEAYRYPAGGLGK
                     P"
     gene            complement(1239..1601)
                     /locus_tag="VEO00_07320"
     CDS             complement(1239..1601)
                     /locus_tag="VEO00_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57843.1"
                     /translation="MIVWLVMLAVLAVAGFDAGSIALTQYKVTTAASDAALEAATVFK
                     QTGDRNQAYKAAVKLVKKDAPGSLIPAKNGFSIDPRTGKATIVVVKRASTLIADRLGF
                     LDSLVRAKAKETSGPATV"
     gene            complement(1646..>2195)
                     /locus_tag="VEO00_07325"
     CDS             complement(1646..>2195)
                     /locus_tag="VEO00_07325"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HYU57844.1"
                     /translation="HGAAHSRSTDLVAGVLVFFSLGLFSFSTFQLLLRAYYSMQDTRT
                     PALVNIAAVGLNVLVDLLFVLALGMGVRGLALGHATAYTFGSFTLLVLIRRRLGGIDG
                     RRILSGLSRTFLAAGITAAVAWVASRAIGGWVDTGTLGGQAAQVLGAVVAGILAFVAS
                     ALMFRVEEVDVVRRQLAARWHR"
BASE COUNT          334 a          794 c          750 g          317 t
ORIGIN      
        1 ggcttcccca ggccacctgc cgggtagcgg taggcctcgg acttccacac cgtgaggggg
       61 tcgtcgtcgg cgacgaagcg aaggcccgag tcgttttcct ggccgtcccc gtacggatcc
      121 atcggggcga cggagatgaa cccgacgact cgagtgtttg gcgccgggct ctcctcggcc
      181 ggtccgaacc ggatgcccac cggcccgccc agttccagct tgccgagcca cagccccgct
      241 ccaagcgcgc tcgccgcgat caccacgacg aggagcggca cggcaagcca tgtccggaac
      301 gtcgagaacc gagggggcga cggcgccggc gcgacggccg cggtctcctc gagccccgac
      361 cggcgcgccg tcgcggccgc ccgctccgtg gccacccccg ccacgtcgtc gagcgcggct
      421 ccgaactcga cggcggtggc gaaacggtcg tcgggctcgg ttgacagcgc ctttcgcaca
      481 acgtcgtcga gcttgcgggt cacgttcggc cggtggtccc tcggggacgc gtcaccggcc
      541 gccggcgtcc ggccggtgag gagctcgtac agcagggcgc ccgcggccca gacgtcggtt
      601 cgaacgtcgg gcgccgcccc ggcggcggtt tcgggtgcga tcggcgccgg ctggaggatc
      661 tcgatcgcct cgtgcggggg catcgaacga acgacggcct cgccgaaccc gagatcgcac
      721 acgcggatcg tcccgtccat gcactcgaag acgttctccg gcttcaagtc gagatgcaaa
      781 acgccggcct cgtgcgcggc ggcgatcgcg tggagcgcgc cacgcaggag gccggcccct
      841 tggtccacgg ggacgttgcc atcgcgggcg atcacggcgc gggcgctgtc gccctcgaag
      901 tactcccgga cgatgaacga gatgccatcg tccttcccag tgtcgaggag ccgagccagg
      961 ccccgatgcg acgccagcgc caccatccga agctccagat cgagtcgctc ggcgaacgcg
     1021 gggtcccggg cgagctccgg gctgatcagc ttcacgacca cgcgccggtc caggacggtg
     1081 tcggtggcgc tccaggaggt gcccgtccca ctcgtgccga gctcctgttc cagggcgtac
     1141 cggccggcga gcacggtggg cggttgctcg gtcctcatcc ggtgagtcta cgtccctgct
     1201 tgcggcgaac gggcgggtgc ctcggaccgc tgaggcgctc acacggtggc ggggcccgac
     1261 gtctccttgg ctttcgccct gaccagggag tcgaggaagc cgagccggtc ggcgatcagg
     1321 gtggaggccc tcttcaccac cacgatggtc gccttgccgg tccgggggtc gatcgagaag
     1381 ccgttcttag cggggatcag ggacccgggc gcgtcctttt tcaccaactt cacggccgcc
     1441 ttgtaggcct ggttccggtc tccggtttgc ttgaagacgg tggccgcttc cagcgccgca
     1501 tccgacgcgg cggtcgtcac cttgtactgg gtgagcgcga tcgagccggc gtcgaagccg
     1561 gccacggcca gcaccgccag catcaccagc cacacgatca ccgtcgttct catgagcccg
     1621 cgttcgtccc ggaccgctgc cgtcttcatc tgtgccacct cgccgccagt tgcctccgca
     1681 ctacatcgac ctcctccacg cgaaacatga gtgccgatgc cacgaatgcg aggatcccgg
     1741 ccactacggc ccccaggacc tgggcggcct gcccgccgag ggtgccggta tcgacccatc
     1801 ccccgatcgc cctggacgcc acccaggcca ccgctgcggt gatgcccgcg gcgaggaacg
     1861 tccgggacag cccggagagg atccgcctcc cgtcgatccc cccgagccgg cgccggatca
     1921 agacgagcag cgtgaacgat ccgaaggtgt aggccgtggc gtgcccgagc gcgaggcccc
     1981 gtacccccat gcccagggcc aggacgaaca gaaggtcgac gagcacgttc aggccgacgg
     2041 ccgcgatgtt caccagcgcc ggtgtcctcg tgtcctgcat cgagtagtac gcccggagca
     2101 gcagctggaa cgtggagaac gagaacaggc cgagggagaa gaacacgagg acccctgcga
     2161 cgaggtccgt gctcctcgaa tgggccgccc cgtgc
//
LOCUS       DASPWO010000318         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_218570, whole genome shotgun sequence.
ACCESSION   DASPWO010000318 DASPWO010000000
VERSION     DASPWO010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218570"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VEO00_07330"
     CDS             complement(<1..429)
                     /locus_tag="VEO00_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYU57845.1"
                     /translation="MKNLCDRLERFSQMKGRHRRNDDSPISVHAHARPCRDRAIVFVL
                     LSTGLRREELIGLDLDQLDPADARSLRAARKVRLVGVRGKGGTQRTVFLSADARHAVA
                     DYLEGERPRDAGPNSTALWLSAMTVASRKRDGQLSGRAVNA"
     gene            complement(413..895)
                     /locus_tag="VEO00_07335"
     CDS             complement(413..895)
                     /locus_tag="VEO00_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HYU57846.1"
                     /translation="MATAQPVESRPQRRRLPSRKDEGGAANLFEAVPDSLEAWIDRYQ
                     SLAVAGVRSGEVAAKIVLHLQRFQLFVVEAYGHDRISTVGRRDVAAWQRHLAAGLAAS
                     TVNNHLASLSGFTTWVAAQAPDVFSAGNPSKGLSGLPLPPLEPRTPVRVAGAVVEEPV
                     "
     gene            1171..1494
                     /locus_tag="VEO00_07340"
     CDS             1171..1494
                     /locus_tag="VEO00_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYU57847.1"
                     /translation="MAEPQRREILVLLRGGERPVTELARDLGMSQSQASKHLRVLREV
                     GLVRVRGAGKQRLYGLDARGLRPVHEWLGGFERFWSGSFDRLDEYVQDLKQARREEPP
                     DGDDQ"
     gene            1478..2029
                     /locus_tag="VEO00_07345"
     CDS             1478..2029
                     /locus_tag="VEO00_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYU57848.1"
                     /translation="MATTSSGGEPEHATADREIVMSRVISAPRELVFEAFTQVRHLSR
                     WWGPEGFSTTTRSFEFRVGGAWDFVMHGPDGTDYQEWITWREIVPPERIALLHGESRD
                     DPNAFESVLAFEPAGDETRIVMRTVFPTKELRDEAVEKYHAIEGGEQTLRNLAAYVAE
                     LTRNDTGRNETTSNETTPKGAES"
     gene            2029..>2247
                     /locus_tag="VEO00_07350"
     CDS             2029..>2247
                     /locus_tag="VEO00_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018798608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HYU57849.1"
                     /translation="MAGKVFFSVTMSLDGFMAPEAVPAEDAFSPEKQDDPGVRRWMSK
                     WSELQAWAFPQRWFRENLKLGEGGEEGID"
BASE COUNT          369 a          753 c          802 g          323 t
ORIGIN      
        1 tgcgttcacc gctctccccg acaactgtcc gtcccgcttc cggctggcca cggtcatcgc
       61 tgacagccac agcgccgtcg agttcgggcc cgcgtcacga ggtcgttccc cctcgaggta
      121 gtcggccacg gcgtggcgcg cgtcggccga caagaacacc gtccgttgcg tgccgccctt
      181 gccccgcacc cccaccaggc ggaccttgcg agctgctcgt aggctgcgtg cgtcggcggg
      241 atccagttga tcaaggtcca ggccgatgag ctcttcgcgg cgcagcccgg tcgaaagcag
      301 cacgaagacg atcgctcggt cccggcaggg gcgggcatgc gcatggaccg agatggggga
      361 atcgtcgttt cgtcggtgcc ggcccttcat ctgtgagaac cgctccaggc gatcacacag
      421 gttcttcaac gaccgcacct gcgactcgga caggggtgcg cggctccagc ggcggcagcg
      481 gcagccccga cagccccttg gatgggttcc cagcggagaa cacgtcgggg gcctgcgcgg
      541 cgacccaggt cgtgaacccc gacagcgagg cgaggtgatt gttcaccgtt gaggccgcca
      601 gcccggcggc caggtgacgc tgccaggcgg ccacgtcccg acgccccacc gtcgagatcc
      661 gatcgtgccc gtaggcctcg acgacgaaca gctggaaccg ctgaaggtgc aacacgatct
      721 tcgccgccac ctcgcccgag cgaacccccg ccactgccag cgactgatag cgatcgatcc
      781 acgcctcgag gctgtcgggc actgcctcga acaggttcgc ggctccacct tcgtcctttc
      841 gtgaaggcaa tctgcggcgt tgtggccggg attcgacggg ttgcgcggtc gccatcggct
      901 gggttccctt cacagaaggg ccgttccgtg caacgaacct accgaccgcc gacaccaagc
      961 tcgcggaccc gacaaagggt cgctcggaac ggcacctcgg gggcatgtct cgcgtcgttg
     1021 tggccgggcg ttcgataaca gtcggcgcct gggaggcgcg tcccgagccg ctcccgggac
     1081 gcggtcccgc ggccggcgtt gacacattcc gatataggca tatgatggcg ccatggcacg
     1141 agcgcgacga cctcggacgc gttcaacgcg atcgccgagc cgcagcggcg ggagatcctg
     1201 gtcctgctgc ggggcggcga gcggccggtg accgagctgg cgcgggacct ggggatgagc
     1261 cagtcgcagg cgtccaagca cctgcgggtg ctccgggagg tcgggctggt gcgggtccgc
     1321 ggggcgggca agcagcggct gtacggcctg gacgcccgcg ggctgcggcc ggtgcacgag
     1381 tggctgggcg gcttcgagcg gttctggagc gggagcttcg accggctcga cgagtacgtg
     1441 caggacctga aacaggcaag gcgggaggaa ccaccggatg gcgacgacca gtagcggcgg
     1501 cgagcccgaa cacgcgaccg cggaccgcga gatcgtgatg tcccgggtca tcagcgcacc
     1561 acgggagctg gtgttcgaag cgttcacgca ggtccggcac ctgtcgcggt ggtggggacc
     1621 ggaggggttc tccaccacca cgcggtcctt cgagttccgc gtcggcgggg cctgggactt
     1681 cgtgatgcac gggccggacg gtaccgacta ccaggagtgg atcacctggc gggagatcgt
     1741 cccgccggag cggatcgcgc tgcttcacgg cgagtcccgc gacgacccca atgccttcga
     1801 gtcggtcctg gccttcgagc cggccggcga cgagacccgg atcgtgatgc gcacggtgtt
     1861 ccccaccaag gaactccgcg acgaggcggt ggagaagtac cacgcgatcg agggcggcga
     1921 gcagaccctg cgcaacctgg cggcctacgt cgccgaactc acccggaacg acaccggccg
     1981 gaacgagacc acctcgaacg agaccacccc gaagggagcg gagagctgat ggccgggaag
     2041 gtgttcttca gcgtgacgat gtcgctcgac ggtttcatgg cacccgaggc cgtgccggcc
     2101 gaggacgcct tctcgcccga gaagcaggac gacccgggcg tcaggcgctg gatgtcgaag
     2161 tggtcggaac tgcaggcgtg ggccttcccg cagcgctggt tccgggagaa cctcaagctc
     2221 ggcgagggcg gcgaggaagg catcgac
//
LOCUS       DASPWO010000319         6628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_979185, whole genome shotgun sequence.
ACCESSION   DASPWO010000319 DASPWO010000000
VERSION     DASPWO010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979185"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VEO00_07355"
     CDS             complement(<1..794)
                     /locus_tag="VEO00_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HYU57850.1"
                     /translation="MGFRFYRSVRLGRGLRLNLSKTGVGVSAGVPGLRYSIHSSGRTV
                     RTAGLPGTGLYYRKDSSVGRSGRSGRSARAALRMPASPRVQMHPKASLFARKEDKLFV
                     RGVTAYMQGRHEDALAALREVIGRDPANAHVGEEVFAGMALVELGRNADAIPMLEAVV
                     ASDQQIPDALMTKYRLSGQMVVAVTPAVTATVPMSHVSAALMLAELYQRAGRPQAAVE
                     LLESLGSLAPDPVFALSLAELYAAMERPDDVLRVTEGFVQNRDDATA"
     gene            879..1562
                     /locus_tag="VEO00_07360"
     CDS             879..1562
                     /locus_tag="VEO00_07360"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYU57851.1"
                     /translation="MTTVRLCAYNVHGFRSGVDVVAEAIAEERPDLLFVNEVGRAWRL
                     RRFARRLGMEPVSGMRLFRKNPNAVLFRAPWRLLEKRIHHLRRHTRLLPRGVVLARLG
                     HAGARLWAGSVHLGLSDGERELHAVELTDLLAGLRPPVVLGGDLNEDPGRRAATWIAG
                     RLWDSFAHAGEGAGETFPCLDPSARIDYLFLSEGITPLKAWVGSGDRVLAASDHRPVF
                     ADVELATLA"
     gene            complement(1732..2523)
                     /locus_tag="VEO00_07365"
     CDS             complement(1732..2523)
                     /locus_tag="VEO00_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYU57852.1"
                     /translation="MTETAPRRSARAAAPARVIPGCEAFRFEGGDLGALVLHGFTGNP
                     TSMRPVGEYLASHGMTVIGPRYPGHGTSIEDLRRTRWPDWVGEAERALDELRARCRDV
                     VVVALSFGASVGVHLAATSPDRLRGLAFVNPHIRDKRLAFAKVGRLFMRTYKGVGNDI
                     KKPGQNEEPYPRIPMPALASMAAFQKLVRRELPSVRLPVIVFHSPDDHVVPPDNARLL
                     LDSIGSPEKELVILPNSYHVATLDYDAETILDRVLVFARDHATDG"
     gene            complement(2520..2924)
                     /locus_tag="VEO00_07370"
     CDS             complement(2520..2924)
                     /locus_tag="VEO00_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYU57853.1"
                     /translation="MTEGERAAGERADAGRKVVLLVEDDESVRKLVRIALELSDYEVV
                     EAADGLEGLLLLDMHQPAAVVLDLMMPDVGGERMLAQLRATEGTRRTPVVIITGKPEV
                     APEVVGMVGEENFFSKPFDPDHLIARIRALTA"
     gene            complement(2921..3052)
                     /locus_tag="VEO00_07375"
     CDS             complement(2921..3052)
                     /locus_tag="VEO00_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57854.1"
                     /translation="MVAFGVLEWSFVGALAVLVGAAGIFALFLLAQLFRNPTRRAER"
     gene            3142..3852
                     /locus_tag="VEO00_07380"
     CDS             3142..3852
                     /locus_tag="VEO00_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005884777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYU57855.1"
                     /translation="MFYWLMKHIFLGPLLKLIYRPKARGLDNIPTSGPAILASNHLSF
                     VDSLFIPLVTKRKVVFLGKADYFDKWYTAWFFKAAGVIPVRRDGGSSSEAALLAGIRA
                     LNDGAVVGIYPEGTRSPDGRLYRGKTGVARMALEAQVPVIPIAVFGTVEVMPIDRKFP
                     RLRGPVSVHFGKPLSFERFYGRPSDRFVLRSVTDEILYEIMMLSGQEYVDEYASKVKQ
                     QLAAERTEAREREPATAG"
     gene            complement(3929..5425)
                     /gene="glpK"
                     /locus_tag="VEO00_07385"
     CDS             complement(3929..5425)
                     /gene="glpK"
                     /locus_tag="VEO00_07385"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HYU57856.1"
                     /translation="MMTVLAVDAGTTGVTALVVAEDGAVRAKGYREFPQSFPRPGWVE
                     HDPDDWWTAFLEAAGEALQAAGVEAAGLAAIGITNQRETTVLWERDTLRPVHPAIVWQ
                     DRRTAPACDVLRDEGWEPRVKERTGLVIDAYFSGTKLAWLLDNVDGARAAAEAGRLAF
                     GTVDAYLLARLTAGAVHATDATNASRTMLYDIHRLRWDDELLQRLTVPAAVLPEVRPS
                     SGRFGVTDPEAFLGARVPISGIAGDQQAALFGQACFEPGATKNTYGTGSFVLMHTGTD
                     AARSASGLLTTIAVGHDGSAEYALEGAIFVTGAAIQWLRDGLGVIASASESGPLAASV
                     EDAGGLYFVPALTGLGAPWWDPYARGTIVGITRGTTRAHLARAAVEAMAYQTRDVVDV
                     MTLEASTPITELRADGGAAAMDLLLQFQADLLGVTVRRPVVQETTALGAAYLAGLGEG
                     VWASREEVAAHWRLDAAFEPGDRAGAERLHEGWLRAVERSRAWAAPDA"
     gene            complement(5441..6373)
                     /locus_tag="VEO00_07390"
     CDS             complement(5441..6373)
                     /locus_tag="VEO00_07390"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HYU57857.1"
                     /translation="MIRKIILGAVLLTVLGSAATAGAGDIDDRVRNAERAQAEARVAV
                     SDVLADLDRLQNEYARAERREARATEALLAVYQRRLVLKAELANARQILAGRARAAYK
                     LGPATALEVILAATNPSELASAQAFTEAAFGSDADAIDLVLTKEDEVRANGQILTAQR
                     KALRAEQTRLADLRAEIEQRLEEAEDTLRAAEKGLAEVRAEKRAIEEAARREAERQRA
                     IEEAKEKQNFEGEADWDAIAACESGGNWGANTGNGYWGGLQFAPETWFGYGGGEFDGK
                     GPFPYAREEQITVADKVLDSQGPGAWPHCFVAAS"
BASE COUNT          873 a         2432 c         2390 g          933 t
ORIGIN      
        1 gccgtggcgt catcccggtt ctggacgaac ccctccgtca cgcgcaggac atcgtcgggc
       61 cgctccatcg ccgcgtacag ctcggcgagc gacagggcga acaccgggtc cggcgcgagg
      121 gagccgagcg actcgaggag ctccaccgcg gcctgcggcc ggccggcccg ctggtagagc
      181 tccgcgagca tcagggcggc ggagacgtgg ctcatcggga cggtggcggt gaccgccggc
      241 gtgacggcga ccaccatctg gcccgacagc cggtacttcg tcatcagcgc gtccgggatc
      301 tgctggtcgg aggcgacgac cgcctccagc atggggatcg catcggcgtt ccggccgagc
      361 tcgaccagcg ccatgccggc gaacacctcc tcgccgacgt gggcgttcgc cgggtcgcgc
      421 ccgatcacct cgcggagggc ggcgagggca tcctcgtggc ggccctgcat gtacgccgtg
      481 acgcccctga cgaagagctt gtcctccttg cgcgcgaaca ggctcgcctt cggatgcatc
      541 tggacccgcg gcgacgccgg catccgaagg gccgccctgg cggagcggcc cgagcgcccc
      601 gagcgcccga cggagctgtc cttgcggtag tacagccccg tcccgggcag gccagccgtc
      661 cggaccgtcc ggcccgatga gtggatcgag taccggagcc cggggacgcc ggccgacacg
      721 ccgacgccgg tcttggacag gttcagccgg aggcccctgc cgagcctgac cgatcggtag
      781 aagcggaatc ccatcccaac cctcgccttc ctccgggctc tatcatgcgc cgcgatgggc
      841 ggcggctctg tggtcgaggt cgacctcgac ctgcgggcgt gacgacggtc cggctctgcg
      901 cgtacaacgt ccacgggttt cgcagcgggg tggacgtcgt tgccgaggcg atcgccgagg
      961 agcgccccga tctgctgttc gtcaacgagg tcggccgggc ctggcgcctt cgccggttcg
     1021 cgcggcggct ggggatggag ccggtctcgg ggatgcgcct gttccggaag aacccgaacg
     1081 cggtcctgtt ccgcgcgccc tggcggctgc tcgagaagcg catccaccac ctgcgccggc
     1141 acacgcgcct gctcccgcgc ggcgtggtcc tggcccggct cggccacgcc ggcgcgcgcc
     1201 tctgggcggg aagcgtgcac ctcgggctgt ccgacggtga gcgcgagctg cacgccgtgg
     1261 agctgaccga tctgctggcc gggctgcggc cgccggtcgt cctcggcggg gacctgaacg
     1321 aggatcccgg ccgccgcgcc gcgacctgga tcgccggtcg actgtgggac tcgttcgccc
     1381 atgccggcga gggtgcgggg gagaccttcc cctgcctcga cccgagcgcg cggatcgact
     1441 acctgttcct ctcggagggg atcaccccgc tgaaggcctg ggtcggctcc ggcgaccggg
     1501 tcctggccgc ctcggaccac cgccccgtct tcgccgacgt cgagctcgcg accctggcct
     1561 gacgcgggtc ccccgagccc cggtgacgct caggagccgt cgccgtcgtt gccgtccgat
     1621 cagggcgagg ctcgcgaccc gcgcgaacgc acggcggccc gcgccatgcg ggagcctgcg
     1681 acgcccgggc tggctctcct gctactcgcg aagggctacc cgtccgaagg gctacccgtc
     1741 cgtggcgtgg tcacgggcga agaccaggac gcgatcgagg atcgtctcgg cgtcgtagtc
     1801 gagcgtggcc acgtgatagc tgttcggcaa gatcaccagc tccttctccg gcgagccgat
     1861 cgagtcgagc agcaggcggg cgttgtcggg cgggaccacg tggtcgtcgg gggagtggaa
     1921 gacgatcacc ggcagccgga ccgacggcag ctcccggcgg accagcttct ggaaggccgc
     1981 catcgacgcc agcgccggca tggggatgcg cgggtacggc tcctcgttct gtcccggctt
     2041 cttgatgtcg ttcccgacgc ccttgtacgt ccgcatgaac agccggccga ccttggcgaa
     2101 cgccagtcgc ttgtcgcgga tgtgcgggtt gacgaaggcg agcccgcgca gccggtcggg
     2161 gctcgttgcg gcgaggtgaa cgcccaccga cgccccgaag gagagggcga cgacgaccac
     2221 atcccggcag cgcgcgcgga gctcgtccag ggcgcgctcg gcctcgccga cccagtccgg
     2281 ccagcgggtc cggcggaggt cctcgatgct cgtcccgtgc cccgggtagc gcggcccgat
     2341 cacggtcatc ccgtgcgagg ccaggtactc gccgaccgga cgcatgctcg tcgggttccc
     2401 ggtgaacccg tgcaggacga gcgcgccgag atcgccgccc tcgaagcgga acgcctcgca
     2461 gccggggatc acgcgggccg gcgcggccgc gcgggccgaa cggcggggag cggtctcggt
     2521 cacgccgtca gcgcccggat ccgggcgatc agatggtcgg ggtcgaacgg cttggagaag
     2581 aagttctcct cgcccaccat gccgaccacc tccggggcga cctcgggctt gccggtgatg
     2641 atcaccaccg gggtgcgccg ggtcccctcc gtggcccgca gctgggccag catccgctct
     2701 ccgcccacgt cgggcatcat caggtcgagc acgaccgcgg ccggctggtg catgtcgagc
     2761 agcagcaggc cctcgaggcc gtccgcagcc tcgaccacct cgtagtcgga cagctcgagg
     2821 gcgatccgga ccagcttgcg caccgattcg tcgtcctcga cgaggagcac gaccttgcgg
     2881 ccggcatcgg cccgctcgcc cgctgcgcgc tcgccctcgg tcaccgctcg gcccgccggg
     2941 tcgggttgcg gaacagctgg gcgagcagga agagcgcgaa gatgcccgcc gcgccgacca
     3001 gcacggccag cgcgcccacg aacgaccact cgagcactcc gaacgccacc atcggcttcc
     3061 gctcctcgac gggctggtct agtctataga gccgtcgccg gccgggcccg ggggccggtt
     3121 ccgcagggtg aggggcgagc gatgttctac tggctgatga agcacatctt cctggggccg
     3181 ctgctgaagc tgatctaccg gccgaaggcg cgcggcctcg acaacatccc gacgtcggga
     3241 ccggccatcc tcgcctcgaa ccacctgtcg ttcgtcgaca gcctgttcat cccgctggtg
     3301 acgaagcgca aggtcgtgtt cctcgggaag gccgattact tcgacaagtg gtacacggcc
     3361 tggttcttca aggcggccgg cgtgatcccc gtgcggcgcg atggcggctc gtccagcgag
     3421 gcggccctgc tcgccggcat ccgcgcgctg aacgacgggg cggtggtcgg gatctacccg
     3481 gagggcacgc ggagcccgga cgggcgcctg taccggggca agaccggcgt ggcccggatg
     3541 gcgctcgagg cgcaggtccc cgtgatcccg atcgcggtgt tcgggacggt cgaggtcatg
     3601 ccgatcgaca ggaagttccc gcgcctgcgg ggccccgtct ccgtccactt cggcaagccc
     3661 ctgtccttcg agcgcttcta cggccgcccg tccgaccgct tcgtcctgcg gtcggtcacc
     3721 gacgagatcc tgtacgagat catgatgctc tcgggccagg agtacgtcga cgagtacgcc
     3781 tcgaaggtga agcagcagct cgcggcggag cgcaccgagg cccgcgagcg cgagcccgcc
     3841 accgccggct agcctccgat ccggacggcg cgccctcatg gaccgagcgc gcgccgcagg
     3901 cgacgcggaa ctcgcggcgg tccgggagtc aggcgtcggg tgcggcccag gcgcgtgacc
     3961 gctcgacggc gcgcagccag ccctcgtgca ggcgctcggc gccggcccgg tcgccgggct
     4021 cgaacgcggc gtccagccgc cagtgcgccg caacctcttc ccggctcgcc cagacaccct
     4081 cgcccaggcc ggccaggtag gcggcgccga gggcggtcgt ctcctggacc accggccgcc
     4141 ggacggtgac gccgagcagg tcggcctgga actgcaggag caggtccatc gccgcggcgc
     4201 cgccgtcggc gcgcagctcg gtgatcggcg tcgacgcctc gagggtcatc acgtccacca
     4261 cgtcgcgggt ctggtaggcc atggcctcaa cggcggcccg ggccaggtgg gcgcgggtcg
     4321 ttccgcgcgt gatcccgacg atcgtcccgc gggcgtaggg gtcccaccac ggcgccccga
     4381 gccccgtcag cgccgggacg aagtacaggc cgccggcgtc ctcgaccgac gccgcgagcg
     4441 gcccggactc cgaggccgag gcgatcaccc cgaggccgtc gcgcaaccat tggatcgccg
     4501 ccccggtgac gaagatcgcg ccctccaggg catactcggc ggagccgtcg tggccgacgg
     4561 cgatcgtggt cagcaggccc gacgcggacc gtgccgcgtc ggtgccggtg tgcatcagga
     4621 cgaaggaccc ggtgccgtag gtgttcttcg tcgcgccggg ctcgaagcag gcctgtccga
     4681 agagcgccgc ttgctggtcg ccggcgatac cggagatcgg gacgcgcgcg ccgaggaatg
     4741 cctcggggtc cgtcaccccg aatcgcccgg aggacgggcg gacctcaggg aggacggcgg
     4801 cggggacggt gaggcgctgc agcagctcgt cgtcccagcg caggcggtgg atgtcgtaga
     4861 gcatcgtccg cgaggcgttc gtggcgtcgg tggcgtggac cgcgccggcc gtcagccggg
     4921 ccagcaggta ggcgtcgacg gtcccaaacg ccagcctgcc ggcctccgcc gccgcccgcg
     4981 cgccatcgac gttgtcgagc agccaggcga gcttggtccc cgagaagtag gcgtcgatca
     5041 ccaggccggt gcgctccttc acccgcggct cccacccctc gtcgcgcagg acgtcgcagg
     5101 ccggggcggt ccggcggtcc tgccagacga tcgccgggtg gaccgggcgg agggtgtcgc
     5161 gctcccagag cacggtcgtc tcgcgctggt tcgtgatccc gatcgccgcg agcccggcgg
     5221 cctcgacgcc cgcggcctgc agcgcctctc cggccgcctc gaggaaggcg gtccaccagt
     5281 cgtcggggtc gtgctccacc cacccggggc gcgggaagct ctgcgggaac tcccggtatc
     5341 ccttcgcccg gacggcgccg tcctcggcga ccacgagcgc cgtcacgccg gtggtgccgg
     5401 cgtcgacggc gagaacggtc atcatagccc cgagcggggg tcaggacgcg gcgacgaagc
     5461 aatgcggcca ggcgcccggt ccctggctgt cgaggacctt gtcggcgacg gtgatctgct
     5521 cctcgcgcgc atacgggaag gggcccttgc cgtcgaactc gccgcccccg tagccgaacc
     5581 acgtctccgg cgcgaactgc agtccgcccc agtagccgtt cccggtgttc gccccccagt
     5641 tgccgccgga ctcgcaggcg gcgatggcgt cccagtcggc ctcgccctcg aagttctgct
     5701 tctctttcgc ttcttcgatg gcccgctgcc gctccgcctc ccggcgggcc gcctcctcga
     5761 tggcgcgctt ctccgcccgc acctcggcca gtcccttctc ggcggcgcgg agggtgtcct
     5821 cggcctcctc gaggcgctgc tcgatctcgg cgcggaggtc cgccaagcgc gtctgctcgg
     5881 cgcgcagggc cttgcgttga gcggtcagga tctggccgtt cgcccggacc tcgtcctcct
     5941 tcgtcagcac gaggtcgatc gcatcggcgt cggagccgaa ggccgcctcg gtgaaagcct
     6001 gggcggaggc cagctcggag gggttcgtcg cggcgaggat gacctcgagc gccgtcgccg
     6061 gcccgagctt gtaggcggcg cgcgcgcgcc cggccaggat ctgccgggcg ttcgcaagct
     6121 ccgccttcag caccagccgt cgctggtaga ccgcgagcag ggcctcggtg gcgcgggcct
     6181 cgcggcgctc cgcgcgcgcg tactcgttct gcaggcggtc gaggtcggcg aggacgtcgg
     6241 agacggcgac gcgagcctcg gcctgggccc gctcggcgtt gcgcacgcga tcgtcgatgt
     6301 cgccggcgcc ggccgtggcc gcgcttccga gcaccgtgag cagcacggcg cccaggatga
     6361 tcttccgtat cacgggaccc cccgatccgg cgcggaaatc ccgccgcacc gaaccctcga
     6421 cgtctacgcg aggttcgctt cgacgagctt cgcctcggac ccttccccgg tcccctggtc
     6481 tgtgggcacg gaccgggagt ctacaatgcc gggcggcacg ggtggtagct tgcaggctcc
     6541 cgacgaatcg gtcgatcaac cccctacgga cgaaaggcgt cttcctggca aggatcggga
     6601 tcctcaccgg cggcggcgac tgccccgg
//
LOCUS       DASPWO010000320        10143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1149023, whole genome shotgun sequence.
ACCESSION   DASPWO010000320 DASPWO010000000
VERSION     DASPWO010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149023"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VEO00_07395"
     CDS             <1..268
                     /locus_tag="VEO00_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYU57858.1"
                     /translation="RADRFLSALPGAGDDVLIFGHGHMLRVLAARHLGLDAGEARHLA
                     LGTGTLSILGHERDWPAVHEWNVPAGLVATVGLAGHAGAEPPVQ"
     gene            278..1495
                     /locus_tag="VEO00_07400"
     CDS             278..1495
                     /locus_tag="VEO00_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU57859.1"
                     /translation="MDFTIPPDLDELRTRVAAFVQDEVLPVESGLPEEATDGDLAAVL
                     GLLREKARAGGLWTPHLPTEWGGVGVGPLGMALVSQECGVSALASLALNAMAPDEGNM
                     HLLLHAGTPEQLERYLRPLAAGETRSCFAMTERAAGADPLGLRTRAVRDGDEWVIDGE
                     KWFITGADGAAFAIVVALTDPRAENPYARYSLLLVDAGTPGWKVVRQVPVMGTHGPGG
                     HCEVLLDGVRVPADALLGAPGAGFLLSQVRLGPARLAHCMRWIGVAQRALDIAAERAR
                     ERETFGEPLANRQAVQWMLADSAIELYAARLMVLHSAWLIERGDDSRQEVSMAKVHVS
                     EIMGNVIDRAIQICGSLGYSKDLPLERFYRDARAARIYDGPSEIHRMVIARNVLKAAR
                     DRGTTKPATGDLA"
     gene            1492..2547
                     /locus_tag="VEO00_07405"
     CDS             1492..2547
                     /locus_tag="VEO00_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HYU57860.1"
                     /translation="MTHAPTGDLSRPLVNPAALSRWLDETLGPGPEISVERHQVGHSI
                     ETFFVIRGEHEWVLRRPPRGAFLPTAHDVLREFRVISALAETPARVPRPVAACADPEV
                     IGAPFYLAERVHGPVIRTEVPAEFASEADRRAIGDEVIDALVEVHAVDWRAAGLEGFG
                     KPAGYLERQLRRWSGQLELTEPFSRRVDEVHEAADWLKANRPESGPSTVVHGDYRLDN
                     AAFAPHPPARLRAIFDWEMSTIGDPLADVGWLVTSWVEPGDPEDAVFGSLAQVTRQRG
                     FPSRADLVARYEERSSRGVLHLEWYMVLALWKLAILLEGSYARHLAGLTDDPFFALLG
                     EGVPTLARRALEIARGG"
     gene            2540..3211
                     /locus_tag="VEO00_07410"
     CDS             2540..3211
                     /locus_tag="VEO00_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HYU57861.1"
                     /translation="MAEPAGAPRLRALLIDYGGVLTTAMGESFAEFAREHDIKAEALL
                     DFLKAGYVEGEADDHPVSRVETGRMELEEFETHLAEALSVGRAEPLDPAGLRDRLFAV
                     LRPDDRMLEAVRRLREAGVKVALVSNSWGPAGYPRDELDTMFDAVLVSGEVGVRKPDR
                     DIFLLAAERLGVEPAACVFVDDIGPNVEGAKAVGMHGIHHRTAGQTLAALELLFGVPL
                     AEAGG"
     gene            complement(3218..3733)
                     /locus_tag="VEO00_07415"
     CDS             complement(3218..3733)
                     /locus_tag="VEO00_07415"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYU57862.1"
                     /translation="MHRSLSLSLLLALLTLSACGGGGPAIGHLSEASTPLPALSGPAV
                     RGGEVQPSDYAGRIVVVNFWASWCAPCRAEQPVLQDMFEELGGPNFTMIGVNYMDDRA
                     SAREFIREFSVTYPSVSDPSARIAARFGVFGLPATFVADASGTIRYQVYGQLDPQELR
                     RAVDALLAAAA"
     gene            complement(3770..4780)
                     /locus_tag="VEO00_07420"
     CDS             complement(3770..4780)
                     /locus_tag="VEO00_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYU57863.1"
                     /translation="MTDETDTPADEEPAPERGAGEDAPRGRLLPVAIPAAAVLGTLIL
                     VFSLSRILLAISQYSKGITAVVALFVALNVLVGAALVSIGRRVRGRRPASFPLLVVAG
                     LLVVGGGAAAIAIGTEPEKEVATEATIVAQTLAFNPTAVELAAGVDTTIHFDNRDGVQ
                     HNIHVFEGQDQSGTSVFVGDLVTGPATADYRLSPQRPGSYHFQCDVHPTMTGTIRFIK
                     VEGQKPRPGPTETETSAPPPKAPPNTVIAKGIQFATPQATATLKGGKVHLVFDNRDAG
                     VPHNVVVFDGSDDSAPQLFRGEIVTGPAQAEYVFEAQPGEYFFHCEVHPTMTGTLTVT
                     GG"
     gene            complement(4777..5661)
                     /locus_tag="VEO00_07425"
     CDS             complement(4777..5661)
                     /locus_tag="VEO00_07425"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HYU57864.1"
                     /translation="MGRVSSPHDRMTALAVGLPTWHPHPSVWVVVLGLEGLYLLALAR
                     HGPRHVEPGERPASGGQIALFTAGVAVVLVGAEWPVHELAERYLYSVHMLQHILFTLV
                     AAPLLLAGTPGWLARALLRPVMPVARVLLRPFFAFVIFNLVLVLSHWPAVVDLTVRSE
                     LAHFVAHAILLGSALIMWWPVFSPIAEIPRLSHPAQMLYLFLQSIIPTVPASFLTFGS
                     TLLYPIYGTFPRLGGITALTDMHVAGLVMKIVGGFILWGVIATLFFRWYHLEQSGEPD
                     VVEWQSVERELNRMRAKS"
     gene            complement(5704..6039)
                     /locus_tag="VEO00_07430"
     CDS             complement(5704..6039)
                     /locus_tag="VEO00_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HYU57865.1"
                     /translation="MSAHGSVGAEVAAVGAHGEQRPHPSVGQYVRVAIILGVVTAGEV
                     GLYYLQAIPDGMLIALLVFFSAIKFSLVALWFMHLRFDSRLLRRLFVTGIILAGSVYA
                     IVLATFLLR"
     gene            complement(6053..6679)
                     /locus_tag="VEO00_07435"
     CDS             complement(6053..6679)
                     /locus_tag="VEO00_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HYU57866.1"
                     /translation="METGIGAHEVVAAEHPPTSTGMSNTKVAMWTFLASECLLFGALI
                     STYLLYRGRVVSGPKPGDVYDIPFTSVSSFVLLMSSLTMVLAVHAIAEGDQAKCRTWL
                     VATALLGMTFISGQVYEFTTFVHEGLALTTNVFSTAFFVLTGFHGAHVTVGIIMLLSL
                     FGLSLDGKIPQSRAETVEMVGLYWHFVDVVWIVIFTLVYLIPSPGGSA"
     gene            complement(6685..8565)
                     /gene="ctaD"
                     /locus_tag="VEO00_07440"
     CDS             complement(6685..8565)
                     /gene="ctaD"
                     /locus_tag="VEO00_07440"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HYU57867.1"
                     /translation="MTTEALPVPRRRFLRRPIAPTGVWSWITTVDHKRIGLLYGFTAF
                     AFFIIGGLEALAIRTQLARPENALLTGDAYNQAFTMHGITMIFLVVMPLSAAFFNYLL
                     PLQIGARDVAFPRLNALSYWLFLFGGIYLYSSFFLGGAPDGGWFGYEPLGRQLAGHGM
                     DYYATGLIILGLASTISAINLLVTILNMRAPGMTLMRMPVFTWMALVVQFLLVFALPV
                     ITVALFQVFFDRTFNTNFYNIANGGDPLLWQHLFWLFGHPEVYILVLPAMGIVSEVLP
                     VFARKPLFGYPVVVFSGIAIGFMGWGVWAHHMFAAGLGPVANSAFALSTMFIAVPTGV
                     KIFNWIATLWKGAIRFTTPLLFAVGFVGLFIIGGLSGVTHAIVPADTQQTDTYYIVAH
                     FHYVLFAGAIFGLFSGIYYWWPKFTGKLLNERLGKTHFWLMFTGANLTFGPMHILGLQ
                     GMPRRIYTYARGLGFDFWNLVATIGAFTIALSILVFIVNVMRTRRRGEASGQDPWDAR
                     TLEWTTPNPTPEYNFAEIPQVHALDEFWHRKYVEDEHGKVVPVPAGGSNGHDDPAAGH
                     GAGHGGHGIHMPSPSYYPVMTAVGPPLIFWGFLYSPLLIVLGAFILLSGVYGWAVEPA
                     SE"
     gene            complement(8562..9578)
                     /gene="coxB"
                     /locus_tag="VEO00_07445"
     CDS             complement(8562..9578)
                     /gene="coxB"
                     /locus_tag="VEO00_07445"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HYU57868.1"
                     /translation="MRNPFRGTRLAALAAVAALLLAACAKNAPQDTLDPAGPVARQID
                     GLFKPVFWIAAGVFFLVEGLLVFALLRFRHRKGRDGVPEQTHGNTRLEIAWTLAPALL
                     LAGIAIPTVGAIFDLAQRPADAVQVTITAKQWWWAVEYPDLGVITANEIHIPVGKRVD
                     FTLKSDNVIHSFWVPRLAGKQDVVPGHVNHLVMEAETPGEYRGQCVEFCGASHANMRL
                     RLFADTPGDFDRWVNEQKADAAVPAEGSLAARGMAIFNDPNLCIRCHAVGGGPGSQVV
                     APSLTHFASRTTFAGSIFENDEANLRRWLADPPAVKPGSLMPDYGLSHEQIDALVAYL
                     ESLK"
     gene            complement(9581..9901)
                     /gene="ccsA"
                     /locus_tag="VEO00_07450"
                     /pseudo
     CDS             complement(9581..9901)
                     /gene="ccsA"
                     /locus_tag="VEO00_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
BASE COUNT         1591 a         3527 c         3617 g         1408 t
ORIGIN      
        1 gcgggccgac cgcttcctct ccgctctccc cggcgccggc gacgacgtcc tgatcttcgg
       61 gcacggccac atgctccggg tgctggccgc ccgccacctc gggctcgacg ccggcgaggc
      121 ccgccacctg gccctcggca ccggaaccct ctcgatcctc gggcacgagc gcgactggcc
      181 ggccgttcac gaatggaacg tcccggccgg gctggtcgcg acggtggggc tggccgggca
      241 cgccggggcc gagccgccgg tacagtaggc cgccgtgatc gacttcacga tcccgcccga
      301 cctcgacgag ctgcggacac gggtcgcggc cttcgtccag gacgaggtgc tcccggtgga
      361 gtccggcctg ccggaggagg ccaccgacgg tgacctggcc gccgtgctgg gcctcctgcg
      421 cgagaaggcc cgcgccgggg gcctgtggac gccgcacctg ccgaccgagt ggggcggggt
      481 cggcgtcgga ccgctcggga tggcgctcgt gtcgcaggag tgcggggtct ccgccctggc
      541 gtcgctggcg ctgaacgcca tggcccccga cgaggggaac atgcaccttc tgctgcacgc
      601 cggcacgccg gagcagctgg agcgctacct gcggcccctg gccgccggcg agacccggtc
      661 ctgcttcgcg atgaccgagc gggcggccgg cgccgacccg ctcggtctgc ggacgcgcgc
      721 ggtgcgcgac ggcgacgagt gggtcatcga tggcgagaag tggttcatca ccggcgcgga
      781 cggcgcggcg ttcgccatcg tcgtggcgct caccgacccg cgggcggaga acccgtacgc
      841 gcgctactcg ctgctgctgg tcgacgccgg cacgccggga tggaaggtgg tgcgccaggt
      901 cccggtgatg ggcacgcacg gcccgggcgg gcactgcgag gtgctcctcg acggcgtgcg
      961 ggtcccggcg gacgcgctgc tcggcgcgcc gggagccggc ttcctgctct cccaggtgcg
     1021 cctcgggccg gcgcggctgg cgcactgcat gcgctggatc ggcgtggcgc agcgcgcgct
     1081 cgacatcgcc gccgagcggg cccgcgagcg cgagacgttc ggggagccgc tggccaaccg
     1141 ccaggccgtc cagtggatgc tcgccgacag cgcgatcgag ctgtacgccg cgcggctgat
     1201 ggtcctgcac tcggcgtggc tcatcgagcg tggcgacgac tctcgccagg aggtctcgat
     1261 ggcgaaggtc cacgtctccg agatcatggg gaacgtgatc gaccgcgcca tccagatctg
     1321 cgggtcgctc ggctactcga aggacctgcc gctggagcgg ttctaccgcg acgcccgcgc
     1381 cgcgcgcatc tacgacggcc cgagcgagat ccaccggatg gtgatcgccc ggaacgtcct
     1441 gaaggccgcc cgcgatcgcg gcacgaccaa gccggccacc ggcgacctgg cgtgacgcac
     1501 gcgccgaccg gcgacctgtc gcgaccgctg gtcaatccgg cggcgctctc gcggtggctc
     1561 gacgagacgc tcgggccggg gcccgagatc agcgtcgagc gccaccaggt ggggcactcg
     1621 atcgagacgt tcttcgtcat ccgcggcgag cacgagtggg tactgcgccg gccgccacgc
     1681 ggagccttcc tgccgaccgc ccacgacgtg ctgcgcgagt tccgggtcat ctcggcgctc
     1741 gccgagactc ccgcccgcgt gccgcggccg gtcgccgcgt gcgccgaccc cgaggtgatc
     1801 ggggcgccct tctacctggc ggagcgcgtg cacggcccgg tgatccggac cgaggtcccc
     1861 gccgagttcg cgtccgaggc cgaccgccgg gcgatcggcg acgaggtgat cgacgcgctg
     1921 gtcgaggtgc acgccgtaga ctggcgcgcc gcgggtctcg aggggttcgg gaagccggcc
     1981 ggatacctgg agcggcagct gcggcgatgg tccgggcagc tcgagctgac cgagccgttc
     2041 tcgcggcgcg tggacgaggt gcacgaggcc gcggactggc tgaaggcgaa ccgtccggag
     2101 tccggaccgt cgaccgtggt ccacggtgac taccgccttg acaacgcggc gttcgcgcca
     2161 caccccccgg cccggctgcg cgccatcttc gactgggaga tgtccacgat cggcgacccg
     2221 ctcgccgacg tgggctggct ggtgacgtcc tgggtcgagc cgggtgatcc ggaggacgcg
     2281 gtgttcggct cgctggccca ggtcacgcgg cagcggggct tcccgtcgcg cgccgatctg
     2341 gtggcgcgct acgaggaacg gtcgagccgc ggggtgcttc acctcgagtg gtacatggtg
     2401 ctggcgctgt ggaagctggc catcctgctg gaggggtcct atgcgcggca cctcgccggg
     2461 ttgaccgacg acccgttctt cgcgctgctg ggcgagggcg tccccacgct ggctcgccgc
     2521 gcactggaga tcgctcgcgg tggctgagcc cgcgggtgcg ccccgcctcc gggcgctgct
     2581 gatcgactac ggcggcgtgc tgaccaccgc gatgggagag tccttcgccg agttcgcccg
     2641 tgagcacgac atcaaggccg aggcgctgct cgacttcctg aaggccggct acgtcgaggg
     2701 cgaggcggac gatcatccgg tcagccgggt cgagacgggc cggatggagc tcgaggagtt
     2761 cgagacgcac ctggccgagg cgctttcggt ggggcgggcg gagcccctcg acccggccgg
     2821 cctgcgcgac cgcctgttcg ccgtcctgcg ccccgacgac cggatgctcg aggccgtgcg
     2881 gcgcctgcgc gaggccggcg tgaaggtggc gctcgtctcg aactcctggg gcccggccgg
     2941 ctacccgcgg gacgagctcg acacgatgtt cgacgccgtc ctcgtctccg gcgaggtcgg
     3001 cgtgcgcaag cccgaccggg acatcttcct gctggcagcg gagcgcctcg gcgtcgagcc
     3061 ggccgcgtgc gtgttcgtcg acgacatcgg tcccaacgtc gagggcgcga aggccgtcgg
     3121 aatgcacggt atccaccacc gcaccgcggg ccagaccctg gccgccctcg agctgctgtt
     3181 cggggtcccg ctggccgagg caggcggctg agcgagccta cgcggcggcg gcgagcaagg
     3241 cgtcgacggc ccggcggagc tcctgcgggt cgagctggcc gtagacctga tagcggatcg
     3301 tgccggacgc gtcggcgacg aacgtggcgg ggaggccgaa gacgccgaac ctcgccgcga
     3361 tgcgggcgct cgggtcgctg acgctcgggt aggtcaccga gaactcgcgg atgaactccc
     3421 gggccgacgc ccggtcgtcc atgtagttca ctccgatcat ggtgaagttc ggaccgccca
     3481 gctcctcgaa catgtcctgg agcaccggtt gctccgcgcg gcagggggcg caccaggacg
     3541 cccagaagtt cacgacgacg atccgtccgg cgtagtccga gggctgcacc tccccgccgc
     3601 ggaccgccgg ccccgacaga gcgggaagcg gggtggacgc ctcggacagg tgtccgatcg
     3661 ccgggccgcc gcccccgcac gcggagagcg tcagcagggc gaggagcagc gagagcgaca
     3721 gcgaccggtg cacgaatccc acgctacacg gcgcgttcgg gaaccgcggc tacccgcccg
     3781 tgaccgtgag tgtgccggtc atggtcgggt gcacctcgca atggaagaag tactcgccgg
     3841 gctgtgcctc gaagacgtac tcggcctgcg ccgggcccgt gacgatctcg ccgcggaaca
     3901 gctgtggcgc gctgtcgtcg gacccgtcga agaccaccac gttgtgcggg accccggcgt
     3961 cgcgattgtc gaacaccagg tggaccttcc cgcccttcag ggtggccgtc gcctgcggcg
     4021 tcgcgaactg gatccccttc gcgatcacgg tgttgggcgg agccttcggc ggcggggcgc
     4081 tcgtctcggt ctcggtcggg ccgggccggg gcttctgccc ctccaccttg atgaaccgga
     4141 tcgttccggt catggtcggg tggacgtcgc actggaagtg gtaggacccg ggacgctggg
     4201 gcgacagccg gtagtcggcc gtggccggcc cggtgaccag gtcgccgacg aagacgctcg
     4261 tccccgattg gtcctgcccc tcgaagacgt ggatgttgtg ctgcacgccg tcgcggttgt
     4321 cgaagtggat ggtcgtgtcg accccggcgg caagctcgac ggccgtcggg ttgaacgcca
     4381 gggtctgggc gacgatcgtc gcctcggtgg cgacctcctt ctcgggctcg gtcccgatgg
     4441 cgatggccgc ggctccgccg cccaccacga gcaggcccgc caccacgagg agcgggaacg
     4501 acgccgggcg ccggccgcgc acgcgccgcc cgatcgagac gagcgcggcg ccgaccagca
     4561 cgttcaacgc cacgaacagg gcgacgaccg cggtgatgcc cttcgagtac tgggagatcg
     4621 ccaacaggat ccgcgacagc gagaacacca ggatcagcgt cccgaggacc gccgccgcgg
     4681 ggatggccac cggcagcagg cggccgcgcg gagcgtcttc ccccgcgccc ctctcgggcg
     4741 ccggctcctc gtccgccggc gtgtcggtct cgtcggtcat gacttcgctc gcatccggtt
     4801 cagctcacgc tcgacgctct gccactcgac gacgtcgggc tcgccgctct gctccaggtg
     4861 gtaccagcgg aagaacagcg tggcgatgac gccccagagg atgaatccgc cgacgatctt
     4921 catcaccagc ccggcgacgt gcatgtcggt cagtgccgtg atcccgccca ggcgagggaa
     4981 ggttccgtag atcgggtaca gcagcgtgct gccgaacgtc aggaacgacg ccggcacggt
     5041 cgggatgatc gactgcagga acagatagag catctgggcc gggtgcgaca gccgggggat
     5101 ctcggcgatc ggggagaaca ccggccacca catgatcagg gccgatccca gcaggatcgc
     5161 gtgggccacg aagtgggcga gctcggagcg caccgtcagg tcgacgaccg ccggccagtg
     5221 cgacagcacc agcacgaggt tgaagatcac gaaggcgaag aagggccgca gcagcacgcg
     5281 cgccaccggc atgaccggcc gcaggagcgc ccgggccagc cacccgggcg tcccggccag
     5341 cagcagcggg gcggcgacga gcgtgaacag gatgtgctgg agcatgtgca cgctgtagag
     5401 gtagcgctcg gccagctcgt gcaccggcca ctcggccccg accagcacca ccgcgacacc
     5461 ggcggtgaac agcgcgatct ggccgcccga ggcgggccgc tcgcccggtt cgacgtgacg
     5521 cgggccatgc cgcgccagcg ccagcaggta gagaccctcc agtccgagga ccaccaccca
     5581 gacgctgggg tgcggatgcc acgtcggcag cccgacggcg agcgccgtca tccggtcgtg
     5641 tggggaggag accctcccca ccccccaccc gtcgggtcgc gccggggagc gctcaccggc
     5701 ccctcatcgc aggaggaacg tggccaggac gatggcgtac accgagccgg ccaggatgat
     5761 cccggtgacg aacaggcggc gcagcaggcg gctgtcgaag cgaaggtgca tgaaccacag
     5821 cgcgacgagg gagaacttga tcgccgagaa gaacacgagc agcgcgatca gcatcccgtc
     5881 ggggatggcc tgcaggtagt agaggccgac ctctcccgcc gtgaccactc ccaggatgat
     5941 ggccacgcgg acgtactggc caacgctcgg gtgcggccgc tgttcgccgt gcgccccgac
     6001 cgccgcgacc tcggcgccga ccgatccgtg cgcgctcatt ccatctcctc gttcacgcgc
     6061 ttcccccggg cgacgggatc aggtagacga gcgtgaagat cacgatccag accacgtcga
     6121 cgaagtgcca gtacaggccg accatctcaa cggtttcggc gcgactctgc gggatcttgc
     6181 cgtcgagcga caggccgaag agcgagagca gcatgatgat cccgaccgtg acgtgggcgc
     6241 cgtggaatcc ggtcagcacg aagaacgccg tgctgaagac gttggtggtc agcgccaggc
     6301 cctcgtgcac gaacgtcgtg aactcgtaga cctggccgga gatgaacgtc atgcccagca
     6361 atgcggtggc caccagccac gtccggcact tcgcctggtc gccctcggcg atggcgtgca
     6421 ccgcgaggac catcgtcagc gacgacatca gcaggacgaa cgacgagacc gacgtgaacg
     6481 ggatgtcgta gacgtcgccg ggcttcgggc cggagaccac ccgcccgcgg tacagcaggt
     6541 aggtcgagat cagcgccccg aacagcaggc actcggaggc aaggaaggtc cacatcgcga
     6601 ccttcgtgtt cgacatcccg gtgctggtcg gcggatgctc tgcggccacc acctcgtgcg
     6661 cgccgatccc cgtctccatg ccgctcactc gctggcgggc tcgacggccc agccgtagac
     6721 gccggagagc aggatgaatg cgccgagcac gatcaacaga ggcgagtaca ggaatcccca
     6781 gaagatcagc ggcgggccga ccgccgtcat caccgggtag taggagggcg acggcatgtg
     6841 gatcccgtgc ccgccgtggc cggctccgtg cccggcggcg ggatcgtcgt ggccgttcga
     6901 cccgccggcc gggaccggga cgaccttccc gtgctcgtcc tcgacgtact tgcggtgcca
     6961 gaattcgtcc agggcgtgga cctgggggat ctcggcgaag ttgtactccg gggtcgggtt
     7021 cggggtggtc cactcgaggg tccgtgcgtc ccacggatcc tgccctgacg cctcgccgcg
     7081 ccggcgcgtg cgcatcacgt tgacgatgaa caccaagatc gacagggcga tcgtgaacgc
     7141 gccgatcgtg gcgacgaggt tccagaagtc gaaccccagc ccgcgggcgt aggtgtagat
     7201 gcgccgcggc atcccctgca ggccaaggat gtgcatcggg ccgaacgtca ggttcgcccc
     7261 cgtgaacatc agccagaagt gcgtcttgcc cagccgctcg ttcagcagct tgccggtgaa
     7321 cttcggccac cagtagtaga tcccactgaa cagcccgaag atcgcgccgg cgaacagcac
     7381 gtagtggaag tgggcgacga tgtagtacgt gtcggtctgc tgcgtgtccg ccggcacgat
     7441 ggcgtgcgtc accccggaca gccccccgat gatgaacagc ccgacgaagc cgaccgcgaa
     7501 cagcagcggc gtcgtgaacc ggatggcgcc cttccagagc gtcgcgatcc agttgaagat
     7561 cttcacgccg gtcggcacgg cgatgaacat cgtcgacagc gcaaaggcgg agttcgccac
     7621 cggccccagc ccggcggcga acatgtggtg cgcccacacg ccccagccca tgaagccgat
     7681 ggcgatcccg gaaaacacga ccaccgggta gccgaacagc ggcttgcgcg cgaacaccgg
     7741 caggacctcg gagacgatgc ccatcgccgg caggaccagg atgtagacct cggggtgccc
     7801 gaacagccag aacaggtgct gccagagcag cgggtctccg ccgttggcga tgttgtagaa
     7861 gttcgtgttg aacgtccggt cgaagaacac ctggaacagg gccacggtga tcaccggcag
     7921 cgcgaacacc agcaggaact gcaccacgag cgccatccag gtgaacaccg gcatccgcat
     7981 cagcgtcatg cccggtgcgc gcatgttcag gatcgtcacc agcaggttga tggcggagat
     8041 cgtggacgcg agcccgagga tgatcagccc ggtggcgtag tagtccatgc cgtgcccggc
     8101 cagctggcgc cccagcggct cgtagccgaa ccagccgccg tcgggcgcgc cgccgaggaa
     8161 gaacgacgag tagaggtaga tcccgccgaa caggaagagc cagtacgaca gcgcgttcag
     8221 ccgggggaac gcgacgtccc gggcgccgat ctgcagcggc agcaggtagt tgaagaacgc
     8281 cgccgacagc ggcatcacca ccaggaagat catggtgatc ccatgcatcg tgaaggcctg
     8341 gttgtaggcg tcgcccgtca gcagggcgtt ctccggccgg gccaattgcg tccggatcgc
     8401 tagcgcctcc aggcccccga tgatgaagaa cgcgaacgcg gtgaacccgt agagcagtcc
     8461 gatccgcttg tggtcgaccg tggtgatcca ggaccacacc ccggtggggg cgatcggacg
     8521 gcgcaggaac cttcgccgcg gaaccggcag ggcttcagtg gtcatttcaa gctctccagg
     8581 taggcgacca gggcatcgat ctgctcgtgg gacagtccgt agtcgggcat cagcgaaccc
     8641 ggcttcaccg cgggcgggtc ggccagccac cgccgcaggt tcgcctcgtc attctcgaag
     8701 atcgagccgg cgaacgtcgt ccggctggcg aagtgggtca ggctcggcgc gaccacctgc
     8761 gatcccggcc cgccccccac cgcgtggcag cggatgcaga ggttcggatc gttgaagatc
     8821 gccatcccgc gggcggcgag cgatccctcg gcgggcacgg cggcgtcggc cttctgctcg
     8881 ttcacccatc ggtcgaagtc cccgggcgtg tcggcgaaca ggcgcaggcg catgttcgcg
     8941 tgactcgcgc cgcagaactc gacgcattgc ccgcgatact cgccgggcgt ctcggcctcc
     9001 atgaccaggt ggttgacatg gccgggcacc acgtcctgtt tccccgccag gcgcggcacc
     9061 cagaacgagt ggatcacgtt gtcgctcttc aacgtgaagt ccacgcgctt gccgaccgga
     9121 atgtggatct cgttcgcggt gatcacgccg aggtcggggt actccaccgc ccaccaccac
     9181 tgcttggccg tgatcgtgac ctgcacggcg tccgccgggc gctgggccag gtcgaagatc
     9241 gccccgacgg tcgggatggc gatgccggcg agcagcaggg ccggcgccag cgtccaggcg
     9301 atctccaggc gcgtgttccc gtgggtctgc tccggcacgc cgtcgcggcc cttgcggtga
     9361 cggaagcgga gcagcgcgaa taccagcagg ccctcgacca ggaagaacac cccggcggcg
     9421 atccagaaga ccggcttgaa caggccgtcg atctgccggg ccaccggccc ggccgggtcg
     9481 agcgtgtcct gcggcgcgtt cttcgcgcag gccgccagca gcagcgccgc gaccgccgcg
     9541 agggcggcga gccgtgtccc ccggaacggg ttccgcatcc ctagaagccc ctatacgagt
     9601 gcaggccgac gatgaacagg ttcacggcgt agtacgtgac catcagcgag gcgaagccta
     9661 tcagcgccag gacggcggct cgccggccct tccagccggc cgtggcccgg gcgtgcaggt
     9721 acccggcgaa gaccacccag gtgatgaagg cccaggtctc cttcgggtcc cagccccagt
     9781 agcggcccca ggcctgctcg gcccagatcg cgccggcgat gaccgcgaac gtccagattg
     9841 ggaacccgat ggcgatcgtc cggtacgcga cccggtcaat caccgcggcc gagggcaacc
     9901 ggccgcgacg ctccgtccgc gtcgggaccg gctcgtcggc gccgggtacg agatcgggtg
     9961 ggttcgccct cgggttcgcc gtcgtgacga tggccgtggc ccggatgcag ttctacgtgg
    10021 tccccgcgcc cctgcagccg gccctcaact cctcctggct caaggtccac gtcatctccg
    10081 cgatcgtcgg gtcgtcgctc ctcacgctgg ggagcctgtt cacgatcctg ttcctggtcc
    10141 agg
//
LOCUS       DASPWO010000321         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_560006, whole genome shotgun sequence.
ACCESSION   DASPWO010000321 DASPWO010000000
VERSION     DASPWO010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560006"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2090)
                     /locus_tag="VEO00_07455"
     CDS             complement(<1..>2090)
                     /locus_tag="VEO00_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HYU57869.1"
                     /translation="ARLSENQGELIVYDTHSPHGLYVNDTRVSGQAPLRDGDVLWLGK
                     PGDETSVMLQCRVARAALPVVVMPEEPSSDVVVFADAGPADAVDPMWDLGDLLAVTPS
                     EPPPPPASPVAAAPEAPSEDVFFMEEPAAPVAPAPPPPPPSPPSPPPPAADDLFFVEE
                     PFDPSVSPPAPAAAPARPAPAPEPPPVPPPKASAAARPAPRPIRRHGPTPPPSAAPER
                     EDEAEPARPRSVPPRPASTPRAAAAPGGRRSSPPVALIAGGAALLLVAIGAGVLFMRS
                     PRAPAIASITPARVGLGQSVTIAGTAFGATPQANVVSFGGKPGRVVQASPTRLQVEIP
                     EVAAVAGRDTPVPVVVLVEGRESKAATVAVFEAPRIHGISPSVAMPGEEVVLAGAGWG
                     AGATVQFGSVAATVLDSSPTSLKVTVPALQGAPGTEFPVRVTTGADTSNPAPFVVGRL
                     PLVLSISPASAAPGDVVTVAGRGFDVKPSANDVRVGGTRALVAAASGSELKVIVPRAA
                     PGETTVDVRLPGAEHVGQSRLVVAAPADPIDLHFVAEPFEDTPGHEHAVLTTGLGPAF
                     VLSASGGRSVAERALEAERRLNEAGAALKGTVDGDVRARGLDGSPSLVVAGRDTSLLD
                     VTIDDAAGYEEDWTGLKDRAGPISPARVAVWWEAVARDLVLLLARGDKPHFAADLAPE
                     GRVLADLHPLARKF"
BASE COUNT          274 a          753 c          829 g          234 t
ORIGIN      
        1 gaacttccgc gcgaggggat ggagatcggc gagcacacgg ccttcggggg ccaggtccgc
       61 ggcgaaatgc ggtttgtcgc cgcgagcgag gaggagcacg aggtcgcggg cgaccgcctc
      121 ccaccatacc gccacccggg cgggtgagat gggccccgcg cggtccttca ggcccgtcca
      181 gtcctcctcg tagccggcgg catcgtcgat cgtcacgtcg aggagcgagg tgtcgcggcc
      241 ggccacgacg agggaaggac tgccgtcgag accgcgcgcg cgcacgtccc cgtcgacggt
      301 gcctttcagc gccgcccccg cctcgttgag ccgccgctcc gcctccagcg cccgctcggc
      361 caccgaccgg ccgccgctgg cgctgagcac gaacgcgggg ccgaggccgg tcgtgaggac
      421 cgcgtgctcg tggccgggcg tgtcttcgaa aggctcggcc acgaagtgca ggtcgatggg
      481 atcggcaggg gcggcgacca cgagccggga ctgcccgacg tgctccgcgc ccgggagccg
      541 gacgtcgacc gtcgtctctc caggagcggc gcgcggcacg atcaccttga gctcgctgcc
      601 ggaggcggcg gccaccagcg cgcgcgtgcc ccctacgcgc acgtcgttgg cggacggctt
      661 gacgtcgaac ccccggccgg ccaccgtcac cacgtcaccg ggggccgcgc tcgcgggcga
      721 gatcgagagc acgagaggaa ggcggccgac gacgaaggga gcggggttgg aggtgtcggc
      781 gcccgtcgtc acgcgcaccg ggaactccgt gccgggcgcg ccctggagcg ccggtaccgt
      841 gaccttgagc gacgtcggcg aagagtcgag cacggtggcg gccacgctcc cgaattgcac
      901 ggttgcgccg gcgccccacc ccgctcccgc cagcaccacc tcctccccgg gcatcgcgac
      961 cgagggcgag atgccgtgga tgcgcggggc ttcgaacacg gccaccgtcg ccgccttgga
     1021 ctcgcgtccc tccacgagga cgacgaccgg tacgggcgtg tcgcggccgg ccacggccgc
     1081 gacctcgggg atctcgacct gaaggcgggt cggcgaagcc tggacgacgc gtccgggctt
     1141 gccgccgaac gagacgacgt tcgcctgggg cgtcgccccg aaagccgtgc ccgcgatggt
     1201 cacgctctgg ccgagcccga cgcgcgccgg cgtgatcgac gcgatcgcgg gcgcacgcgg
     1261 cgagcgcatg aacaggacac ccgcccctat tgcgacgagg aggagcgccg cgccgcccgc
     1321 gatgagggcg acgggcggtg aagaccgccg tccaccgggg gcggcggccg cccgtggcgt
     1381 ggacgccggg cgtggcggca cgctgcgcgg ccgcgccggc tcggcttcgt cctcccgctc
     1441 cggcgcggcc gaaggaggcg gtgtcggacc gtggcggcgg atcggccgtg gggccggccg
     1501 ggcggcggcg ctcgccttgg gaggcggcac cgggggcggc tccggcgccg gagccgggcg
     1561 cgccggggcg gccgcggggg cggggggaga caccgacggg tcgaacggct cttcgacgaa
     1621 gaacaggtcg tctgccgcgg gcggcggagg cgagggaggc gagggaggcg ggggcggcgg
     1681 ggcgggcgcg acgggtgcgg cgggctcctc catgaagaac acgtcctcgc tcggcgcttc
     1741 gggcgcggcc gcgaccgggg aggccggcgg cggcggcggc tccgagggcg tgacggccag
     1801 gaggtcgccc agatcccaca tcggatcgac ggcgtcggcc ggccccgcgt cggcgaacac
     1861 gaccacgtcg gaggaaggct cctccggcat cacgaccacg ggcagggcgg cccgggccac
     1921 ccggcattgg agcatgacgc tcgtctcgtc gccgggcttc ccgagccaga gcacgtcacc
     1981 gtcccgcagc ggcgcctgtc cgctcacgcg cgtgtcgttg acgtacagcc cgtgtgggct
     2041 gtgcgtgtcg tagacgatca actccccctg gttctccgag aggcgcgcgt
//
LOCUS       DASPWO010000322         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1233493, whole genome shotgun sequence.
ACCESSION   DASPWO010000322 DASPWO010000000
VERSION     DASPWO010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233493"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VEO00_07460"
     CDS             complement(<1..1063)
                     /locus_tag="VEO00_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HYU57870.1"
                     /translation="MATAAVTQALEEHWGEKPGLGKVLSTVDHKSIGKHYVYTAFVFF
                     VLGGIQALIMRAQLASGDERLIGPETFNELFSMHGITMIFFFVTPMLFGFGNYFVPLM
                     IGTRDMAFPRLNAFGYWIFLFSGVFLYTSYIAGAAPNDGWFNYVPLSSIQYTPGLNID
                     YYTLGLAFLGISTTVGAVNFIVTILKLRAPGMTLSRMPLYCWAILATSFAVVFALPSL
                     TSANIMLQLDRRAGFHFFDVAKGGDAILWQHLFWIFGHPDVYIIFLPAVGIVSTIVPA
                     FARRRIIGYVWLALATMTTAIIGFGVWVHHMFAVRLPQITMVFFAAASLFVVVPSGIQ
                     IFAWCATLVRGRPRLETPLL"
     gene            complement(1063..2010)
                     /gene="coxB"
                     /locus_tag="VEO00_07465"
     CDS             complement(1063..2010)
                     /gene="coxB"
                     /locus_tag="VEO00_07465"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HYU57871.1"
                     /translation="MLGACTGSPSTLDAQGPGAARIANLWWVMFGISVVVIAVVSGLL
                     AAAILRRRRRQLDPDAEPRWGLRLILWGGAILPVVVLTAMWVLTLRDLRAISEPISRG
                     EVTIEVIGHRWWWEVRYPSLGIVTANDVHIPVGRPVTVRLQTIDVIHSFWVPQLTGKT
                     DMIPGRTNSMSIQADRSGVYRGQCAEFCGLQHANMAFFVVADEPAAFQAWATREAGPA
                     PAPTDPRARLGLQVFLQQPCVACHAIRGTDATGAIGPDLTHFGSRLSIGAGTVPNERG
                     FLGGWIENSQALKPGNLMPPMRLDAEDLDGLLAYLESLT"
     gene            2199..>3664
                     /locus_tag="VEO00_07470"
     CDS             2199..>3664
                     /locus_tag="VEO00_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYU57872.1"
                     /translation="MRPGSILGHPVLRVAAPVLVTGAARYVDDVAASGALHAVFVRAT
                     VAHGRITSIDAGTALGMDGVAGVFLAADLDLPPLASGMVPTGFSRPILAPGVVRFVGE
                     PVAVVVATTRALAIDAAESVLVDYEPLPVVIDPQAAEAADAPLLFPDHGTNVAMRSPF
                     GAGLGAEAEDEVVVRARFVNQRLAPIPMEGNGALAVPDPKAGGLTLWVSCQAAHYWRA
                     GIAELIGLAEHQLRVIAPWVGGGFGAKIDVNPEHVAVCAVALRLGRAVRWFETRSENL
                     TAMTHGRAQIQDVEIAGRRDGTITRLRAGLLADGGAYPGGAAYLPVLTQRMLSGGYRI
                     PRIDGEIVCVCTNTTPINAYRGAGRPEAAALLERAVDMFGAEIGMDPAEVRRVNFVPK
                     DAFPYTTATGAVYDSGDYEKALDEALAAAGYRGLRREQAERRARRDPIQLGIGLSAYV
                     EVTAFGLGSEFGSVEVRPDGSVVVLAGTTPQGQGHVTA"
BASE COUNT          584 a         1238 c         1318 g          524 t
ORIGIN      
        1 agaggagcgg cgtctcgagc cgcggccgcc cgcggacgag cgtcgcgcac caggcgaaga
       61 tctggatccc gctcgggacg acgacgaaca ggctcgcggc ggcgaagaag accatcgtga
      121 tctgcggcag cctgaccgcg aacatgtggt gcacccacac cccgaacccg atgatcgcgg
      181 tggtcatcgt ggccagggcc agccagacgt agccgatgat cctgcgccgg gcgaacgccg
      241 ggacgatcgt cgacacgatg ccgacggccg gcaggaagat gatgtagacg tccgggtggc
      301 cgaagatcca gaacaggtgc tgccacagga tcgcgtcgcc gcccttcgcg acgtcgaaga
      361 agtggaaccc ggcccggcgg tccagctgga gcatgatgtt cgccgaggtc agggagggaa
      421 gcgcgaacac caccgcgaac gaggtggcca ggatcgccca gcagtacagc ggcatcctgg
      481 agagcgtcat gccgggcgcc cggagcttga ggatcgtgac gatgaagttg acggcgccga
      541 ctgtggtcga gatgccgagg aaggccagcc ccagcgtgta atagtcgatg ttcaggccgg
      601 gcgtgtactg gatgctcgac agcggcacgt agttgaacca cccgtcgttc ggcgcggccc
      661 cggcgatgta gctcgtgtac aggaacaccc ctgagaacag gaagatccag tacccgaacg
      721 cgttgagccg ggggaatgcc atgtcccggg tgccgatcat cagcggcacg aagtagttcc
      781 cgaacccgaa gagcatcggg gtgacgaaga agaagatcat cgtgatcccg tgcatcgaga
      841 acagctcgtt gaacgtctcg ggcccgatca gccgctcgtc ccccgaggcc agctgggccc
      901 gcatgatcag cgcctggatg ccgcccagga cgaagaacac gaaggccgtg tagacgtagt
      961 gcttgccgat cgacttgtgg tcgaccgtgg acaggacctt tcccaggccc ggtttctccc
     1021 cccagtgctc ctccagcgcc tgggtgaccg cggcggtggc cactacgtca ggctttcgag
     1081 gtaggccagc aggccgtcga ggtcctcggc gtccaaccgc atcggcggca tgaggttccc
     1141 gggcttcagg gcctgggagt tctcgatcca cccgcccagg aagccgcgct cgttggggac
     1201 ggtgcccgcc ccgatgctca ggcgggatcc gaagtgggtc aggtcggggc cgatcgcccc
     1261 ggtcgcgtcg gtcccgcgga tcgcgtggca cgccacgcaa ggttgctgca agaagacctg
     1321 gagtcccagc cgggcccgcg gatccgtcgg agccggagcg ggcccggcct ccctcgtcgc
     1381 ccacgcctgg aacgccgccg gttcgtccgc cacgacgaag aaggccatgt tcgcgtgctg
     1441 gagcccgcag aactccgcgc actgtccccg gtacaccccg gatcggtcgg cctggatgct
     1501 catggagttg gtgcgcccgg ggatcatgtc ggtcttcccg gtgagctggg ggacccagaa
     1561 gctgtggatc acgtcgatcg tctgcagccg gaccgtcacc ggccgcccca ccgggatgtg
     1621 gacgtcgttg gcggtgacga tcccgagcga cgggtaccgg acctcccacc accatcggtg
     1681 tccgatcacc tcgatcgtaa cctcgccccg ggagatgggc tcgctgatgg ctcgaaggtc
     1741 ccgcagggtg agcacccaca tcgccgtgag gaccacgacc gggaggatcg ccccgcccca
     1801 caggatcagg cgaagccccc accgcggctc ggcgtcgggg tcgagctgcc ggcgacggcg
     1861 ccggaggatc gccgcagcca ggagccccga gacgacggcg atgaccacca ccgagatccc
     1921 gaacatgacc caccacaggt tcgcgatgcg cgccgcgccc gggccctggg cgtcaagcgt
     1981 cgagggtgag cccgtgcacg cgccgagcac cgacgtggcg acgacgacga ggactcgcgc
     2041 ccggggtgat gagcgagacc gggcgacgag aggttccggg ggcatctccg gcgatacctt
     2101 cccccggggg cgccgcccct agtctcggga cctcagaggg ggacggcaaa tcaagcccgg
     2161 gcttccgccc ggcacccgct tcctctaccc tgcgccacgt gcgacccggt tcgatcctcg
     2221 ggcacccggt ccttcgggtc gcggcccccg tcctcgtgac gggggccgca cggtacgtgg
     2281 acgacgtggc ggccagtgga gccctccacg ccgtgttcgt ccgggccacg gtggcccacg
     2341 gccggatcac ctcgatcgac gccgggaccg cgctcggcat ggacggggtg gccggagtgt
     2401 tcctcgccgc cgacctggac ctccctccgc tggcgtccgg gatggtgccg accggtttct
     2461 cgcgcccgat cctggccccg ggcgtggtcc ggttcgtcgg ggagccggtc gcggtggtcg
     2521 ttgccacgac ccgggcgctc gccatcgatg cggcggaatc ggtgctggtg gactacgagc
     2581 cacttccggt ggtgatcgat ccccaggcgg ctgaggccgc ggatgccccg ctcctgttcc
     2641 ccgaccacgg aacgaacgtc gcgatgcgga gccccttcgg cgctgggctc ggagcggagg
     2701 cggaggacga ggtcgtggtc cgggcccgct tcgtgaacca gcggttggcg ccgatcccga
     2761 tggaaggcaa cggagcgctg gccgttcccg acccgaaggc gggcggcctc acgctgtggg
     2821 tctcgtgcca ggcagcccac tactggcggg ccggcatcgc cgaactgatc gggttggctg
     2881 agcaccagct tcgggtgatc gctccgtggg tgggcggcgg gttcggcgcg aagatcgacg
     2941 tgaatcccga gcacgtcgcg gtgtgcgcgg tggcgctccg gctcggccgg gcggtccggt
     3001 ggttcgagac ccgctcggag aacctcaccg cgatgaccca tggccgggcc cagatccagg
     3061 atgtcgagat cgccggacgc agggacggga cgatcacccg cctccgggca ggcctcctgg
     3121 ccgacggggg cgcgtatcca gggggtgccg cctacctgcc ggtactgacg cagcggatgc
     3181 tgtccggcgg ctatcgcatt ccccggatcg acggagagat cgtgtgcgtc tgcacgaaca
     3241 ccaccccgat caacgcctat cggggagcgg gacgcccgga ggccgccgcc ctgctcgagc
     3301 gggcggtcga catgttcggt gccgagatcg ggatggaccc ggccgaggtt cgtcgcgtga
     3361 acttcgtccc gaaggacgcg ttcccgtaca cgacggcgac gggcgccgtg tacgactccg
     3421 gcgactacga gaaggcattg gacgaagcgc tcgccgccgc cgggtaccgc gggctcaggc
     3481 gggagcaggc cgaacggcgg gctcgtcgcg acccgatcca actggggatc gggctgtccg
     3541 cgtacgtgga ggtcacggcg ttcgggctcg ggtccgagtt cggatcggtc gaggttcggc
     3601 ccgacggctc ggtggtggtc ctcgccggaa ccacgcccca gggacagggg cacgtcaccg
     3661 cgtt
//
LOCUS       DASPWO010000323         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_897153, whole genome shotgun sequence.
ACCESSION   DASPWO010000323 DASPWO010000000
VERSION     DASPWO010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897153"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VEO00_07475"
     CDS             <1..276
                     /locus_tag="VEO00_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016882455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HYU57873.1"
                     /translation="IVVDHIARQMDDPEFRKLAMAVFEDTIAPYVRDRGFDWEIHFDE
                     TAIDLWRVQGLIAPPENSDMEKLWAKENRPVPYDYDTMLPLEPVTTP"
     gene            375..1592
                     /locus_tag="VEO00_07480"
     CDS             375..1592
                     /locus_tag="VEO00_07480"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HYU57874.1"
                     /translation="MPEAVIVAHTRSPIGRAMKGSLKDIRADDLLAQVVESVLADVPA
                     LDREELGDLILGCGLPGGEQGWNMARRVAVQLGMDSLPGTTITRYCSSSLQSTRMAFH
                     AIKAGEGTAYISAGVESVSSFVRGSSDHFPGTENPLFDTARERTAARKLSNVPWSDPR
                     EDGLVPDVYLDMGETAENVASLTGISRERQDEWAVRSQHRAEEATKSGHFTSEITAVT
                     LPDGTVVSADDCPRFGTTYEAVSQLKPVFREGGSVTAGNSCPLNDGAAALVIMSDVRA
                     RELGLTPLARIISTGVTGLSPEIMGLGPIEATKQALAHAGMSVGDIDLAEINEAFAVQ
                     VLGSADALKLDEDKLNVNGGAIALGHPFGMTGARITGTLINNLRAHDKTIGLETMCVG
                     GGQGMAMLIERLA"
     gene            1601..>2136
                     /locus_tag="VEO00_07485"
     CDS             1601..>2136
                     /locus_tag="VEO00_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004800 - thyroxine 5'-deiodinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deiodinase-like protein"
                     /protein_id="HYU57875.1"
                     /translation="MDAAPVLDESSDVGRRYRIARPTLGTVLSDLRIPGDQPGPGDEI
                     PRLDLPTTDGGRFSNPTVAAGGRPVLLVFGSLTCPITESAGAGLVALHRKYGDAVDFV
                     VVNVREAHPGAAVPQPEVAEAKLRNARALRSHHGFEFPVAVDDLDGGVHRAFGTRPSS
                     AYLIDATGTILFRAHWSNR"
BASE COUNT          340 a          766 c          719 g          311 t
ORIGIN      
        1 atcgtggtgg accacatcgc gcgccagatg gacgaccctg agttccgcaa gctcgccatg
       61 gcggtcttcg aggacacgat cgcgccgtac gtgcgggacc gcggcttcga ctgggagatc
      121 cacttcgacg agacggccat cgacctgtgg cgcgttcagg gactcatcgc gccgccggag
      181 aactccgaca tggagaagct ctgggccaag gagaaccggc cggtgcccta cgactacgac
      241 accatgctcc ccctcgagcc cgtcaccacc ccatgaccga ggacctgggg cacgggtcga
      301 ctcccgtgcc ccgggacctc ttccaccctt cccccgagtc gtccgaccac cacccgacaa
      361 gagagtgatc agccatgcct gaagccgtca tcgtcgccca cacccgttcg ccgatcgggc
      421 gggccatgaa gggttccctc aaggacatcc gcgcggacga cctcctcgcg caggtcgtcg
      481 agtccgtgct tgccgacgtc cccgcgctcg atcgtgagga gctcggcgac ctgatcctcg
      541 gttgcggtct cccggggggc gagcagggct ggaacatggc caggcgggta gcggtccagc
      601 tcgggatgga cagccttccc ggcaccacca tcacgcgcta ctgctcctcc tcgctccagt
      661 cgacgcggat ggccttccac gcgatcaagg ccggcgaggg cacggcgtac atctcggccg
      721 gcgtcgagtc ggtgtccagc ttcgtgcgcg gcagctccga ccacttcccg ggcacggaga
      781 accccctctt cgacaccgcg cgggagcgta cggcggcgcg caagctgagc aatgttccct
      841 ggagcgatcc ccgtgaggac gggctggtcc cggacgtcta cctcgatatg ggtgagaccg
      901 ccgagaacgt cgcgtccctg acggggatct cgcgcgagcg gcaggacgag tgggccgtgc
      961 ggtcgcagca ccgggcagag gaggcgacga agagcggcca cttcacgtcc gagatcaccg
     1021 cggtcacact tcccgacggg acggtggtct cggccgacga ctgcccgcgg ttcggtacga
     1081 cgtacgaagc ggtctcgcag ctgaagccgg tgttccgcga ggggggatcg gtcaccgcgg
     1141 gcaactcctg cccgctcaac gacggtgcgg ccgcactcgt gatcatgtcg gacgtccggg
     1201 cgcgtgagct cgggctcacc ccgctggccc ggatcatctc cacgggcgtc accgggctct
     1261 ccccggagat catgggcctg ggtccgatcg aggcgaccaa gcaggccctc gcgcacgccg
     1321 ggatgagcgt cggtgacatc gacctggccg agatcaacga ggcgttcgcg gtgcaggtgc
     1381 tcggctccgc cgacgcgttg aagctcgacg aggacaagct gaacgtgaac ggcggcgcga
     1441 tcgccctcgg tcaccccttc ggcatgaccg gcgcccggat cacgggcacc ctgatcaaca
     1501 acctccgggc ccacgacaag accatcgggc tggagaccat gtgtgtcggg ggcggccagg
     1561 gcatggcgat gctgatcgag cggctcgcct gagagtcgcc atggacgcag cacccgtgct
     1621 cgacgagtct tcggacgtgg gccgccggta ccgcatcgcg cggcccacgc tggggaccgt
     1681 cctgagcgat ctgcggatcc ccggcgacca accgggaccg ggcgacgaga tcccccgcct
     1741 cgaccttccg accacggacg gcggacggtt cagcaacccg accgtggccg ccgggggccg
     1801 gcccgtgctg ctggtgttcg ggtcattgac ctgcccgatc accgagagcg cgggggccgg
     1861 tctggttgcc ctccaccgga agtacggcga cgcggtggac ttcgtggtgg tcaacgttcg
     1921 cgaggcgcat cccggagccg ccgtcccgca gccggaggtc gccgaggcga agctgcgcaa
     1981 tgctcgcgcc ctgcgcagcc accacgggtt cgagttcccc gtcgccgtcg atgacctcga
     2041 cggcggcgtc caccgcgcgt tcggcacccg ccccagctcg gcgtacctca tcgacgcgac
     2101 cggaacgatc ctcttccgcg cgcactggtc caaccg
//
LOCUS       DASPWO010000324         3960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_470066, whole genome shotgun sequence.
ACCESSION   DASPWO010000324 DASPWO010000000
VERSION     DASPWO010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470066"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VEO00_07490"
     CDS             <1..387
                     /locus_tag="VEO00_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HYU57876.1"
                     /translation="MAPHGGGCFSGKDPTKVDRSAAYMARYAAKNVVAAGLARRCQLQ
                     VAYAIGTAHPVSLNVDTFGTEEADPARIEAAVREAFDFRPAAIIRDLDLLRPIFRETA
                     AYGHFGRKGFPWEETDRAEDLRRLAG"
     gene            416..2251
                     /locus_tag="VEO00_07495"
     CDS             416..2251
                     /locus_tag="VEO00_07495"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57877.1"
                     /translation="MRVPRLALDRPFTYRLPEGLEAPTGSLVAVPFHGRVVEGWVLGP
                     ADEIPDRVADVRGLRSGVRFFDERLLALYRWFGQRYVTPLASAIDRGIPPRVVSEEPF
                     PVAPAPGPPPGPRPERVVRYEGGGHLAAALEGGSGTFLVRPLPDDEAALAIEAIGRCL
                     AGGRDAVLLVPEAHPLPHTAAAVRDAFGDAVCVFLQGEPRARYRAWLDVLGGRYRVVI
                     GSRPAVFAPLPSLGLVWISREAHAGHREDRSPAYHVRDVGAARARLAGAVLVAASLCP
                     SAEAVADPSLEVVRAGRDAEQAAWPVVETVRPGPEGRARRLVALLKSARSGFLLSSRP
                     GYGVARVCRACGEPAACAACGGVLEQARGRVTCRACGAPGRCAHCGATDFGVQRRGAE
                     RVAEWAARIAPVPVREGAAGPAPGAVTVGTAAAVNDVGPLRLDVVGILDADRMRLRPG
                     LAGPEQALATWFEAAAWAGPRGGGHVVVETAHPGDPVVQALVRSDPWYFHARERARRE
                     EAGFPPGFPVFRVRGGAGLTDRLEALDPLHLLVAGEGGGLGGVARPGASDTRPPTAGE
                     PPKENEGERVCLVTVRPESLAAFGRTMRDLARAGEVTRVDAEPHL"
     gene            2360..2887
                     /gene="def"
                     /locus_tag="VEO00_07500"
     CDS             2360..2887
                     /gene="def"
                     /locus_tag="VEO00_07500"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HYU57878.1"
                     /translation="MPIRTFGHPVLRERARPVESFDDNLRRLAEDMLETMYAAPGVGL
                     AANQIGMAIRLIVWDDGNGPGAMANPVVSRLEGEQLDDEGCLSVPGLYYPTARAFHVR
                     AEGLDLFGTPVAHDAEGLLARIFQHEVDHIEGKLFLSRLSRENRADALAAMRDRELGI
                     ERNGRGLRPRPSRAT"
     gene            2900..3832
                     /gene="fmt"
                     /locus_tag="VEO00_07505"
     CDS             2900..3832
                     /gene="fmt"
                     /locus_tag="VEO00_07505"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HYU57879.1"
                     /translation="MRVAFLGSDRWSVPSLEALVAGGHDVIAFTRPPKPAGRGNRLTP
                     TPVADAARAHGMAVVEPPTVTAGEGMASLADPRPDVLVVVAYGEILPPGVLAVPRTIP
                     VNVHFSLLPELRGAAPVQHAILRGLPATGVTTIRMDAGVDTGPILLQAEEAIDPEDDA
                     GVLGARLAAVGAALLVETLARLAAGTLEERPQDDAAATLAPRLSPEDRVLDWSEPAER
                     LWRRVRALAPEPGATARFRGKVLKVFRSRPHAAPPEGPTPPGTVAVVTRDGLAVATGD
                     GLLALEEVGPEGRARMSGGEFVSGYRPQLGEALQ"
BASE COUNT          430 a         1467 c         1550 g          513 t
ORIGIN      
        1 atggctccgc acggaggggg ctgcttctcg ggcaaggacc ccaccaaggt cgaccgctcg
       61 gccgcgtaca tggcccgcta cgcggcgaag aacgtggtcg ccgccggcct ggcccggcgc
      121 tgccagctgc aggtggccta cgccatcggg acggcccacc cggtgtcgct gaacgtcgac
      181 accttcggga ccgaggaggc agacccggcg cggatcgagg cggccgtccg cgaagcgttc
      241 gacttccgcc ctgcggcgat catccgggac ctcgacctgc tccgaccgat cttccgcgag
      301 accgccgcct acgggcactt cggccggaag ggcttcccct gggaggagac cgaccgcgcc
      361 gaggacctcc gccgcctcgc cggctaaccc gcccgaccgc cacgtcgcgg tcgccgtgcg
      421 ggtcccgcgc ctggcactcg accgcccgtt cacctaccgc ctcccggagg gcctcgaggc
      481 gccgaccggc tcactcgtcg cggtgccgtt ccacggccgg gtcgtcgagg gctgggtgct
      541 cggcccggct gacgagatcc ccgaccgcgt cgccgacgtc cgggggctgc gctcgggcgt
      601 gcggttcttc gacgagcgcc tgctggcgct ctaccggtgg ttcggccagc gctacgtcac
      661 gccgctggcc agcgcgatcg accgcgggat ccctccgcgc gtggtctcgg aggagccgtt
      721 cccggtggcg cccgcgccgg ggccgccccc cgggcctcgg ccggagcggg tcgtccgcta
      781 cgagggcggc gggcacctgg ccgcagcgct cgagggtggg agcgggacgt tcctggtccg
      841 gccgctgccc gacgatgagg cggcgctggc gatcgaggcc atcggccggt gcctcgcggg
      901 cgggcgcgac gccgtgctgc tggtccccga ggcgcacccc ctcccgcaca ccgccgccgc
      961 cgtccgcgat gctttcggcg acgcagtctg cgtcttcctg cagggagagc ctcgggcgcg
     1021 gtaccgggcg tggctggatg tcctcggcgg ccggtaccgc gtggtgatcg gctcccggcc
     1081 ggccgtgttc gccccgctcc cgagcctcgg cctggtgtgg atcagccgcg aggcccacgc
     1141 cgggcaccgc gaggaccgct cgccggccta ccacgtgcgc gacgtcggcg cggccagggc
     1201 ccgtctggcc ggcgcggtgc tggtggccgc ctcgctctgc ccctcggcgg aagcggtggc
     1261 cgatccgtcc ctcgaggtgg tgcgggccgg gcgggacgcc gagcaggccg cctggccggt
     1321 ggtcgagacg gtccggccgg gcccggaggg gcgggctcgg cggctcgtcg cgctgctgaa
     1381 gtcggcgcgc tcggggttcc tgctgtcgag ccgcccgggg tacggcgtgg cccgcgtctg
     1441 tcgcgcctgc ggcgagccgg ccgcctgcgc cgcctgcggc ggcgtgctcg agcaggcccg
     1501 cggccgggtg acgtgccggg cctgtggcgc ccccgggcgg tgcgcccact gcggcgcgac
     1561 cgacttcggg gtgcagcgcc ggggcgcgga gcgcgtcgcg gagtgggccg cgcggatcgc
     1621 gccggtcccg gtacgcgagg gcgcggccgg tccggccccc ggcgccgtga ccgtggggac
     1681 cgccgcggcg gtgaacgacg tcgggccgct gcgcctcgat gtcgtcggca tcctggacgc
     1741 cgaccggatg cggttgcggc cgggcctggc cgggcccgag caggcgctgg ccacctggtt
     1801 cgaggcggcg gcctgggccg ggccgcgcgg gggggggcat gtcgtcgtcg agaccgccca
     1861 tccgggcgat cctgtcgtcc aggcgctggt gcgctccgac ccgtggtact tccacgcccg
     1921 cgaacgtgcg cggcgcgagg aggccggctt cccgccgggg ttccccgtct tccgcgtccg
     1981 tggcggcgca ggcctgaccg accggttgga ggcgctcgac cccctgcacc tgctggtcgc
     2041 cggggagggc ggggggcttg ggggggtagc tcgccctggg gcgagtgaca ctcgaccacc
     2101 gacggcgggc gaacccccca aggagaacga gggcgagcgg gtatgcttgg tcaccgtccg
     2161 tcccgagtcg ctcgccgcgt tcggccggac gatgcgcgac ctcgcccgcg cgggcgaggt
     2221 cacccgcgtc gatgccgaac cgcacctctg atcgagatcc cggggagggt tggggggtag
     2281 ctcgccctgg gacgagtggc actcgaccac cgtcggcagc gaaccccccg gggagaagga
     2341 gccaggaccg gtgagcgtga tgcccatccg aacgttcggc cacccggtgc tgcgcgagcg
     2401 tgccaggccc gtcgagtcct tcgacgacaa cctgcgccgc ctggccgagg acatgctcga
     2461 gacgatgtat gccgcacccg gggtcgggtt ggcggcgaac cagatcggca tggcgatccg
     2521 gctgatcgtc tgggacgacg ggaacgggcc cggggcgatg gcgaacccgg tggtctcacg
     2581 cctcgagggc gagcagctgg acgacgaggg gtgcctgtcc gtgcctgggc tctactaccc
     2641 gacggcccgc gccttccacg tccgggccga gggcctggat ctgttcggaa cgccggtcgc
     2701 ccacgatgcg gagggcctgc tggcgcggat cttccagcac gaggtcgacc acatcgaggg
     2761 gaagctgttc ctgtcgcggc tctcccggga gaaccgggcg gacgccctgg ccgcgatgcg
     2821 cgaccgggag ctcggcatcg agcgcaacgg tcgcggcctc cgcccccggc cgtctcgcgc
     2881 gacctagggg cgaccgagcg tgcgggtcgc gttcctgggg agcgaccgct ggtcggtgcc
     2941 ctccctcgag gcgctcgtgg cgggggggca cgatgtcatc gccttcaccc ggccgccgaa
     3001 gccggccggg cgcggcaacc gcctgacgcc gaccccggtc gccgacgccg cgcgggcgca
     3061 cggcatggcc gtcgtcgagc caccgaccgt gacggcgggg gaggggatgg ccagcctggc
     3121 cgacccgcgg ccggacgtgc tggtggtggt cgcctacggc gagatcctgc cgccgggcgt
     3181 gctcgccgtc ccgcgcacga tcccggtgaa cgtccacttc tccctgcttc cggagctccg
     3241 cggggcggct cccgtccagc acgcgatcct gcgcgggctg ccggccaccg gcgtgacgac
     3301 gatccgaatg gacgccggcg tggacaccgg cccgatcctg ctgcaggcgg aggaggccat
     3361 cgaccccgag gacgacgccg gcgtgctcgg cgctcgcctg gccgcggtcg gcgctgccct
     3421 gctggtcgag acgctcgccc gcctcgcggc cggcacgctc gaagagcggc cgcaggacga
     3481 cgccgcggcg accctcgcgc cgcgcctgtc cccggaggat cgcgtgctcg actggtcgga
     3541 gcccgccgag cgcctctggc gacgggtgcg ggccctggcg ccggagcccg gcgcgaccgc
     3601 acggttcagg gggaaggtgc tgaaagtctt ccggagccgc ccgcacgccg ccccgcccga
     3661 ggggcccacc ccgccgggga cggttgcggt cgtcacgcgg gacgggctgg ccgtcgccac
     3721 cggcgacggg ctgctggcgc tggaggaggt cggcccggag gggcgcgcgc ggatgagcgg
     3781 gggtgagttc gtcagtggct accggcccca actgggggag gcgctccagt agggaggcgc
     3841 ggcgggttgc ctaccgcacc atccgccggg tgctcgagtc cagtgcgtac tcgaatctcg
     3901 cgctcgccgc cgagctgtcg cgggtgacgc tcccgcggat ggaccgtgac ctggcctcag
//
LOCUS       DASPWO010000325         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_389809, whole genome shotgun sequence.
ACCESSION   DASPWO010000325 DASPWO010000000
VERSION     DASPWO010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389809"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1649)
                     /locus_tag="VEO00_07510"
     CDS             complement(114..1649)
                     /locus_tag="VEO00_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HYU57880.1"
                     /translation="MYGRVTGVAVVGIEGHLIQVEAHVGRGLPALALTGLPGAGVQDA
                     RERVRPAVEGSGLEWPLRRVTINLSPADLRKEGPGFDLPVAMAVLAACSQVKQDRLER
                     HVLFGELSLTGALVRTPGVLAVAVAAVRAGMAGVIVPEANAPEAALVGGLEIVGAPSL
                     ARVTDFLRGEWRPPSPNPGASEAREDGVDLAEVRGQAAARRAFEVAAAGGHNLLVVGP
                     PGAGKTMLARRLPTILPALDREEALEVTRLHSVAGLLPAGAGLVGHRPFRNPHHTVSP
                     AGLLGGGSGFLRPGEVSLAHHGVLFLDEVTEVRHDALEGLRQPLEEGRVVITRASGTV
                     AFPARFTLVAAANPCPCGYQGDPRRRCECLPHRVLHYRQKLSGPLLDRIDLRLSVPRL
                     TRAELMGSAQGEASAAVRARVEAARERGRARLAGTPFHANGQMPGSLARRTVRLTGAA
                     RELLAGAVDALALSGRGFDRALRVARTVADLAGSGTVEADHLAEALAYRADDRVGAPA
                     GAA"
     gene            complement(1817..>2067)
                     /locus_tag="VEO00_07515"
     CDS             complement(1817..>2067)
                     /locus_tag="VEO00_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HYU57881.1"
                     /translation="IDLVVRRADLLAFVEVKTRGGDRFGGPFEAVGHRKQHKLRQLGE
                     AFLATSPGAGRLRCRFDVASVTLRAGGRAEVHVFEDAF"
BASE COUNT          272 a          838 c          741 g          216 t
ORIGIN      
        1 ggccagcacc agcgccgccc tccgttccgg cccgcgctcc gcgaaccccg gcggccagcc
       61 ctgaaccgtc ggtccgtcgc gggcgcgatc ctccataagc ccgggagcgc ggctcatgcc
      121 gctcccgccg gcgcgccgac ccggtcgtcg gcccgatagg cgagcgcctc cgccaggtgg
      181 tcggcctcga cggtccccga gccggccagg tctgccacgg tccgcgccac gcgcagcgcg
      241 cggtcgaacc cgcgaccgga gagggcgagc gcgtccaccg caccggccag cagctcgcgc
      301 gcggcgccgg tcagccggac ggttcgccgg gccagcgagc cgggcatctg gccgttggcg
      361 tggaacgggg taccggcgag gcgagcccgg ccgcgctcgc gggccgcctc gacccgggcg
      421 cgaacagcgg cggacgcctc cccctgggcc gagcccatca gctccgcccg ggtgagccgc
      481 ggcacggaga ggcgcaggtc gatccggtcg aggagggggc cggagagctt ctggcggtag
      541 tgcaggaccc ggtgcggcag gcactcacac cgccggcggg ggtcgccctg gtagccgcac
      601 gggcacgggt tggccgcggc aacgagggtg aaccgcgccg ggaacgccac ggtccccgac
      661 gctcgcgtga tcaccacgcg cccctcctcg agcggctggc gcaggccctc cagcgcgtcg
      721 tggcgcacct cggtgacctc gtcgaggaac aggacgccgt ggtgcgccag cgagacctcc
      781 ccgggccgaa ggaagccgct cccgccaccg aggaggcccg ccggagacac cgtgtggtgc
      841 gggttgcgga acggccggtg cccgacgagc ccggcgccgg ccgggagcag gccggccacc
      901 gaatgcaggc gcgtcacctc gagggcctcc tcccggtcga gcgcagggag gatcgtcggc
      961 aggcgccggg ccagcatggt cttcccggcg cccggcgggc cgaccaccag caggttgtgg
     1021 ccgccggcgg cggccacctc gaaagcgcgg cgcgccgccg cctggccacg cacctctgcc
     1081 aggtcgaccc cgtcctcacg cgcctcggac gcgccggggt tcggggacgg gggacgccat
     1141 tcgccgcgca ggaaatccgt cacccgggcg agcgagggcg cgccgacgat ctccaggccc
     1201 ccgaccagcg ccgcctcggg cgcgttcgcc tcgggcacga tcacaccggc catcccggcc
     1261 cgcaccgcgg cgacggccac ggccaggacc ccgggggtgc gcacgagagc gccggtcagc
     1321 gagagctcgc cgaacagcac gtgccgctcg aggcggtcct gcttcacctg cgagcaggcg
     1381 gcgagcaccg ccatcgccac cggcaggtcg aaccccggac cctccttgcg caggtcggcc
     1441 ggcgacaggt tgatcgtcac tcgccgaagc ggccactcga gccccgagcc ctcgaccgcc
     1501 ggccgcaccc gctcgcgggc gtcctgcacg cccgctcccg gtaggcccgt cagcgccagc
     1561 gcggggagcc cccgcccgac gtgcgcttcg acctggatca gatggccctc gatcccgacc
     1621 accgccaccc cggtcacccg cccgtacatt tgcccccttt ggggtccgct cgctggacgt
     1681 gctcgcctga acgtctccca gggcgagcta cctcccaacc ccggcgggac gctatcgcgc
     1741 gccaccgaca cggccggccg ccaggcggct gacctgggcg gacggttccc ggacgtcgtc
     1801 gtctcctccg cgcggcctag aacgcgtcct cgaagacgtg gacctcggcc cggcccccgg
     1861 cacggagggt cacgctggcg acgtcgaagc ggcagcgcag ccgcccggcg cccggcgacg
     1921 tggccaggaa cgcctcgccc agctgccgca gcttgtgctg ctttcggtgc ccgaccgcct
     1981 cgaacggccc gccgaaccga tcgccgcccc gcgtcttgac ctcgacgaag gcgagcaggt
     2041 cggcgcgccg gaccaccaga tcgatct
//
LOCUS       DASPWO010000326         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_470491, whole genome shotgun sequence.
ACCESSION   DASPWO010000326 DASPWO010000000
VERSION     DASPWO010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470491"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..651
                     /locus_tag="VEO00_07520"
     CDS             223..651
                     /locus_tag="VEO00_07520"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HYU57882.1"
                     /translation="MDDPRELVHAILKMVNDNDLDRAEELIHEDYVDHPGGGMPDMHG
                     PEGFRQIVAMFRAGFPDMEVRPEEVIVEGDLAAWRTVGTGTNTGELMGMPATGKRVTF
                     TALDMGRMQDGKAIEHWSGVDMVGMMQQLGMMPEMGKAAG"
     gene            complement(673..2016)
                     /locus_tag="VEO00_07525"
     CDS             complement(673..2016)
                     /locus_tag="VEO00_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU57883.1"
                     /translation="MQPETRYTAVGDSQIAYQVIGEGPRDLVLMMSFLNHIDVRWEEP
                     SLSRFIRRLTSFSRVILFDRRGIGASDPLPGGRTASWEDWLDDIVAVMDAAGSKRATI
                     LAFGDATPLALLFAATHQERVESLALWGAFASALRTDEHPWGQSQEQADAFVELVRQH
                     WAREDDVVLRVFAPSKVGDEPFSHWHAKLRRAGLTPRRAAEVLDLISRIDVTPALPLI
                     SVPTCVMTPDTFSIVPPQEGEFVASRIPGAKHVPMPGVDMLVYASGADRVLDLVEEFV
                     TGERAGARADRVLATVLFTDIVGSTEQAVQKGDRRWREVLDTHDDVARQVVERHDGRL
                     VKTTGDGVLATFDGPARAVRAAAAMRDALRRAGVEIRAGLHAGEVERRGDDVGGIAVH
                     IAARVMAQAGPGEVLCSATVKGLVAGSGLEFEDRGEHTLKGVPDRWPLYALVAGA"
BASE COUNT          323 a          773 c          739 g          258 t
ORIGIN      
        1 gagcctccac gtgcggaacg cggcgaacag gcccccgagg accagggcgc cccccacgat
       61 gacgaagatc aggtccacga caccacctcc cggtgcggat cgtcccatac gccgggctcg
      121 agcaccgcgc ggaccagccc gaacgggtct ggcggcgcgg ggcccgagtt cctatcgtgg
      181 agctcgaccg atgggcgccc gctggggaag agaggagacg agatggacga tccgcgcgag
      241 ctggtgcacg cgatcctgaa gatggtgaac gacaacgacc tggacagggc cgaagagctg
      301 atccacgagg actacgtcga ccacccgggc ggcggcatgc ccgacatgca cggccccgag
      361 gggttccggc agatcgtggc gatgttccgc gcagggttcc cggacatgga ggtccgcccc
      421 gaggaggtca tcgtcgaggg cgacctggcc gcgtggcgca ccgtgggcac cgggacgaac
      481 accggcgagc tgatggggat gccggcgacg ggcaagcgcg tcacgttcac cgcgttggac
      541 atgggacgga tgcaggatgg caaggccatt gagcactgga gcggggtcga catggtcggc
      601 atgatgcagc agctcggcat gatgcccgag atggggaagg ccgccggctg aaccgccgcc
      661 gctccggcga tatcaggcgc cggcgacgag cgcgtagagg ggccagcggt cggggacgcc
      721 cttcagggtg tgctcgccgc ggtcctcgaa ctcgagaccc gatcccgcca ccaggccctt
      781 cacggttgcg ctgcacagga cctcgccggg cccggcctgg gccatcaccc gcgccgcgat
      841 gtgcacggcg atgccgccga cgtcgtcgcc gcgccgctcc acctcgccgg cgtgcagccc
      901 ggcgcggatc tcgacgccgg cccgccgcag cgcgtcgcgc atcgccgccg cggcgcgcac
      961 cgcgcgggcc ggcccgtcga acgtcgccag gacgccgtcg cctgtcgtct tcacgagccg
     1021 cccgtcgtgg cgctcgacca cctgccgtgc gacgtcgtcg tgcgtgtcga gcacctctcg
     1081 ccagcgccgg tcgcccttct gcacggcctg ctccgtggag ccgacgatgt cggtgaacag
     1141 caccgtcgcc agcacgcggt cggcgcgcgc cccggcccgc tcgccggtga cgaactcctc
     1201 gaccaggtcc agcacgcggt cggcacccga cgcgtagacc agcatgtcca cgccgggcat
     1261 cgggacgtgc ttcgcccccg ggatgcgcga ggcgacgaac tctccctcct gcggcggcac
     1321 gatcgagaac gtgtcgggcg tcatcacgca cgtcggcacg ctgatcagcg gcagggcggg
     1381 ggtcacgtcg atccgcgaga tcaggtcgag gacctccgca gcccggcgcg gggtgagccc
     1441 ggcgcggcga agcttcgcgt gccagtggct gaacggctcg tccccgacct tcgagggggc
     1501 gaacacgcgc aggacgacgt cgtcctcccg cgcccagtgc tgccgcacca gctccacgaa
     1561 cgcgtcggcc tgctcctgcg actgccccca ggggtgctcg tcggttcgca gcgccgaggc
     1621 gaacgcgccc cacagcgcga gcgactcgac gcgctcctgg tgcgtggcgg cgaacaggag
     1681 cgcgagcggc gtggcgtcgc cgaaggcgag gatcgtggcc cgcttcgagc cggcggcgtc
     1741 catcaccgcc acgatgtcgt ccagccagtc ctcccacgac gcggtccggc cccccggcag
     1801 cggatcggac gccccgatcc cccgccggtc gaacaggatc acccggctga acgacgtcag
     1861 ccgccggatg aaccgcgaca gcgagggttc ctcccaccgc acgtcgatgt ggttcaggaa
     1921 cgacatcatc agcaccaggt cgcgcggtcc ctcgccgatg acctggtacg cgatctggga
     1981 gtcccccacg gcggtgtaac gggtctccgg ttgcaccccc cgagcctacg cgccgccgag
     2041 tcgacccgcc cggcgccgcc gccagagact acgggcgtga tcccggatac gcg
//
LOCUS       DASPWO010000327         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_560725, whole genome shotgun sequence.
ACCESSION   DASPWO010000327 DASPWO010000000
VERSION     DASPWO010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560725"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1141)
                     /gene="gnd"
                     /locus_tag="VEO00_07530"
     CDS             complement(113..1141)
                     /gene="gnd"
                     /locus_tag="VEO00_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HYU57884.1"
                     /translation="MQLGMVGLGRMGSNLVRRLMRAGHECVVSDVNHAAVERMETEGA
                     VGTANLEEFVQRLQPPRVAWVMVPAVFAGDTILDLRRLMQPGDIVIDGGNSNYRDDLE
                     RARALAERGIHFVDVGTSGGVFGLDRGFCLTIGGEPEIVAHLDPIFAALAPGVDAAPR
                     TPGRQGGAPSTAEAGYLHCGPFGAGHFVKMVHNGIEYGMMAAYAEGLAILRRANAAME
                     AQDQDAETAPLRGEQYYHYEIDTAEVAEAWRRGSVVTSWLLDLTAAALQESPTLEEFS
                     GRVSDSGEGRWTIEAAIELGVPANVLTAALFERFASQGGADFAGRVQSALRQQFGGHL
                     EKPAGSGR"
     gene            1219..>2476
                     /locus_tag="VEO00_07535"
     CDS             1219..>2476
                     /locus_tag="VEO00_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HYU57885.1"
                     /translation="MSLRAIADYALISDCHSAALVSTDGSIDWLCFPRFDRPSVFGRL
                     VDDRAGHFSITAADATEVSRRYRGRTMVMETTFRTPSGVAVLVDAMALDGRGEAHDLG
                     AGAPGALLRRIEGREGTVEVEVEYAPRPEYGLIAPLLTGNEAGVAGRGGADLLLLSAD
                     RAPEIDGATARFRFSLQAGESASFCLDHASAGEGAPRAWDATAVADGLKGTVRAWRKW
                     SAAHQAYEGPWADLVYHSGRVLQALTYQPTGAVIAAPTTSLPETVGGERNWDYRYAWV
                     RDASFTLDALWVAACPDEARAFFGWITGAVASGLAEGRDLQIMFGVGGEHDLTERTLP
                     HLSGWRDSRPVRVGNGAWNQRQLDVYGELLDAALRLQDQLADLDPATKRFLIAVVDAS
                     AARWQETDQGIWEVRDEPRHFVYSKLM"
BASE COUNT          313 a          901 c          921 g          341 t
ORIGIN      
        1 ccgcgcgccg ggtcctgcgc gaacaggtcg cgcaggtgga ggtgccggac ggcctcgaag
       61 tgggcggcga gcgcgcgcca ctccgccgtc tcggtgaccg agcccgccgc ggtcaccggc
      121 ccgagcccgc cggcttctcc aggtggccgc cgaactgctg gcgcagggcg gactggaccc
      181 ggccggcgaa gtcggccccg ccctgtgagg cgaaccgctc gaagagcgcg gcggtcagca
      241 cgttggccgg cacgccgagc tcgatcgcgg cctcgatcgt ccaccggccc tcgccggagt
      301 cggacacccg accgctgaac tcctcgagag tggggctctc ctgcagggcc gccgcggtca
      361 ggtcgagcag ccaggaggtc acgacgctgc cgcgccgcca ggcctcggcc acttccgcgg
      421 tgtcgatctc gtagtggtag tactgctcgc cacgaagggg agcggtctcg gcgtcctgat
      481 cctgcgcctc catggccgcg ttcgcccggc gcaggatcgc cagtccttcg gcgtaggcgg
      541 ccatcatccc gtactcgatc ccgttgtgga ccatcttcac gaagtgcccg gcgccgaacg
      601 gaccgcagtg caggtagccg gcctcggcgg tgctcggggc gccgccctgg cgacctggcg
      661 tgcgcggcgc ggcgtccacg cccggggcga gcgccgcgaa gatcgggtcg aggtgggcca
      721 cgatctccgg ctcgcccccg atcgtcaggc agaagccgcg gtccaggccg aagacgccgc
      781 cgctggttcc gacgtcgacg aagtggattc cgcgctcggc cagggcgcgg gcccgttcga
      841 ggtcgtcgcg atagttcgag ttgccgccgt cgatgacgat gtcgcccggc tgcatcagcc
      901 ggcggaggtc gaggatcgtg tccccggcga agacggccgg gaccatcacc cacgccaccc
      961 gcggcggctg caggcgttgc acgaactcct cgaggttcgc ggtcccgacg gcgccctcgg
     1021 tctccatccg ctccaccgcc gcgtggttca cgtcggagac gacgcactcg tgcccggcgc
     1081 gcatcagccg ccgcacgagg ttcgacccca tccggccgag ccccaccatc ccgagctgca
     1141 tgctggcccc cccgttcctc cgttcgatga ttcccgcgta gtctccccgc ccgggggaga
     1201 tggtgcacgg gtgacggagt gagcctgagg gcgatcgccg actacgcgct gatctcggac
     1261 tgccactcgg cggccctggt gtcgacggac ggctcgatcg actggctgtg cttcccgcgg
     1321 ttcgaccgcc cgagcgtctt cggccggctc gtggacgatc gcgccggcca cttctcgatc
     1381 accgccgccg acgctaccga ggtgagccgg cgctaccggg gccggacgat ggtcatggag
     1441 accacgttcc ggacgccgtc cggcgtcgcg gtgctcgtgg acgccatggc cctcgacggc
     1501 cgcggcgagg cgcacgacct cggggccggg gcgccgggcg cgttgctgcg ccggatcgag
     1561 ggccgcgagg gaacggtcga ggtcgaggtg gagtacgccc cgcggccgga gtatggcctg
     1621 atcgcgccgc tgctcaccgg gaacgaggcg ggcgtggccg gacgcggcgg cgcggacctg
     1681 ctgctgctct cggccgaccg ggcgccggag atcgacggcg ccacggcgcg gttccgattc
     1741 tcgctgcagg cgggggagtc cgcctcgttc tgcctggacc acgccagcgc cggtgagggc
     1801 gcgccgcggg cctgggacgc caccgcggtc gcggacgggc tgaagggcac cgtccgagcc
     1861 tggcggaagt ggtcggcggc gcaccaggcc tacgaggggc cgtgggcgga cctcgtgtac
     1921 cactcgggcc gggtcctgca ggcgctgacc tatcagccga ccggcgccgt catcgccgcg
     1981 ccgacgacct cgctcccgga gacggtcggc ggggagcgca actgggacta ccgctacgcc
     2041 tgggtccggg acgcgagctt cacgctcgac gcgctctggg tggcggcctg cccggacgag
     2101 gcgcgggcct tcttcggctg gatcaccggc gcggtggcct cggggctcgc cgaggggcgc
     2161 gacctgcaga tcatgttcgg ggtcggtggg gagcacgacc tgaccgagcg gacgctgccg
     2221 cacctctcgg gttggcgcga cagccggccg gtgcgggtcg ggaacggcgc gtggaaccag
     2281 cgccagctcg acgtctacgg cgagctgctc gacgcggcgc tgcgcctgca ggaccagctc
     2341 gccgacctcg accccgccac caagcgcttc ctgatcgccg tggtcgacgc ctcggcggct
     2401 cgctggcagg agaccgacca gggcatctgg gaggtgcggg acgagccgcg ccacttcgtc
     2461 tactccaagc tgatgt
//
LOCUS       DASPWO010000328         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_219696, whole genome shotgun sequence.
ACCESSION   DASPWO010000328 DASPWO010000000
VERSION     DASPWO010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219696"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VEO00_07540"
     CDS             <1..335
                     /locus_tag="VEO00_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYU57886.1"
                     /translation="RDDPYPDLEAKAGALVEGLASGLESAGVRHAIERTGSLFSLFFT
                     EGPVRDYEDARAADHARFAAFHRAMLERGVYLPPSGYEAWFLSTAHGDAEIERVVAAA
                     AEAARLTA"
     gene            complement(464..1216)
                     /locus_tag="VEO00_07545"
     CDS             complement(464..1216)
                     /locus_tag="VEO00_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57887.1"
                     /translation="MIREVAAGLTPFESVKGVLAVALGIVIFVGSVYLVVSAVTGRVL
                     GYLITAVGFFGMMIIFSVIFVIGVPGSTPPNQGPRGPEAHWVTVAAGEEISSPEFPDV
                     AEYPGRPWQAPNDETRAEVEGVGTSIKEFLAERANEELGLTEDEAEEILSKGPSTDAV
                     ADTQFTVLNVKFLTVGDTELAAGQAYFTTGGPVVSAFAVFDRGRVPMFSWAFLIGSVI
                     LFAVHVPFLDRAERKRKEILTGGTAPPFLGPA"
     gene            complement(1213..1863)
                     /locus_tag="VEO00_07550"
     CDS             complement(1213..1863)
                     /locus_tag="VEO00_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYU57888.1"
                     /translation="MAGVAVSGGVVLGLVAGAVAAAAILAGAVIVARRRPETLDIPPA
                     MGPGPADEALERRNLEKLMGWGALSFIVMAIWVPVLWLREPGQNVTDENGQTRTAIDR
                     GEQTTQNFQEGVNEFGFSCVRCHGTGLQGGTNYFQGGFVTVPRLTDVCGRLTIDQVKT
                     TIMEGRANTDMPSWSVRFAGPLDDQQINDILQYLISVQTVPDEDNLCINPQAGATP"
     gene            complement(1863..2570)
                     /locus_tag="VEO00_07555"
     CDS             complement(1863..2570)
                     /locus_tag="VEO00_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYU57889.1"
                     /translation="MSGLGISSQTFGLLLVGALFGLFLLLLAASFLRARRAGALERAG
                     RPAEPPPAAAPKPISRRDFFRGSLLGSMLVFLAQFGGASLAFLYPNLRGGFGSVIAAG
                     TVQEIKNQISDSRAPFYLGAGRFYIVQYDVDPVPTEYEGIAAGGLMALYQRCVHLGCR
                     VPFCEQSQWFECPCHGSKYNRAGEYQLGPAPRSLDRFPIVLDGDTVMVDTGKLQTGPP
                     RGTNTLRQRPEGPFCVA"
     gene            complement(2567..>2971)
                     /locus_tag="VEO00_07560"
     CDS             complement(2567..>2971)
                     /locus_tag="VEO00_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HYU57890.1"
                     /translation="HLLIEEFVSLLVVTAFLIVFSTFVNAPLRELANPNLTPNPSKAP
                     WYFLGLQELLRYFHPMVAGVLMPTLAILGLIVVPYLDRNPSTKPGDRKLAITIFTLFI
                     LFWSILVIIGSFFRGPGYNFVFPWQGGLFFEL"
BASE COUNT          476 a         1051 c         1037 g          407 t
ORIGIN      
        1 aacgggacga tccctacccg gatctggagg cgaaggccgg ggcgctggtg gaagggctgg
       61 cgtcggggct ggagtccgcc ggggtccgcc acgccatcga acggaccggt tcgctgttct
      121 cgctgttctt caccgagggt ccggtgcgcg actacgagga cgcccgggcc gccgaccacg
      181 cccggttcgc cgcgttccac cgggcgatgc tcgagcgcgg ggtctatctg ccgccgtcgg
      241 gctacgaggc gtggttcctg tcgacggccc acggcgacgc cgagatcgag cgcgtggtcg
      301 cggcggccgc ggaggccgcc cggctgacgg cctgatccgc tacttcggga tggtcagcgc
      361 caggacgacg aagatcccga cgagcatcag cgagaagagg atccaggcgc tcccgccctc
      421 gacgatcggc tcgaaaccca gcatgcgctc tcccttgcga tggctacgcc ggcccgagga
      481 acggcggcgc cgtccccccg gtcaggatct ccttgcgctt gcgctcggcc cggtccagga
      541 acggcacgtg caccgcgaac aggatcaccg agccgatcag gaaggcccac gagaacatcg
      601 gcactcgccc gcgatcgaac acggcgaacg cgctgaccac cgggccgccc gtcgtgaagt
      661 aggcctgccc ggcagcgagc tccgtgtcgc ccaccgtcag gaacttcacg ttcagcaccg
      721 tgaactgcgt gtccgccacc gcgtcggtgc tcgggccctt cgacagaatc tcctccgcct
      781 cgtcctcggt cagcccgagc tcctcgttcg cccgctcggc caggaactcc ttgatcgagg
      841 tgccgacgcc ctcgacctcg gcgcgggtct cgtcgttcgg cgcctgccag ggccttccgg
      901 ggtactcggc cacgtcgggg aactccggcg agctgatctc ctcgccggcg gcgaccgtca
      961 cccagtgcgc ctcggggcca cgggggccct gattcggtgg cgtgctcccc gggacgccga
     1021 tcacgaagat gaccgagaag atgatcatca tcccgaagaa gcccaccgcg gtgatcaggt
     1081 agccgagcac ccggccggtc acggcggaga cgaccaggta gacgctgccg acgaagatca
     1141 cgatgcccag ggcgaccgcc aggacgccct tgacggactc gaacggggtc aggcccgccg
     1201 ccacctcgcg gatcacggcg tggcccccgc ctgcgggttg atgcacaggt tgtcctcgtc
     1261 ggggacggtc tggaccgaga tcaggtactg caggatgtcg ttgatctgct ggtcgtcgag
     1321 cggcccggcg aagcgcacgc tccacgacgg catgtcggtg ttcgcgcgcc cctccatgat
     1381 cgtggtcttg acctggtcga tcgtcagccg gccgcacacg tcggtcagtc gcggcacggt
     1441 gacgaacccg ccctggaagt agttcgtgcc gccctgcagg ccggtgccgt ggcagcggac
     1501 gcaggagaac ccgaactcgt tgacgccctc ctggaagttc tgcgtggtct gctcgccgcg
     1561 atcgatcgcc gtgcgcgtct ggccgttctc gtcggtgacg ttctggccgg gctcgcgcag
     1621 ccacagcacc ggcacccaga tcgccatgac gatgaacgac agcgcgcccc agcccatcag
     1681 cttctccagg ttccgccgct cgagcgcctc gtcggccggt ccggggccca tcgccggcgg
     1741 gatgtcgagc gtctccggcc gccggcgcgc cacgatgacc gccccggcca ggatcgccgc
     1801 ggcggccacg gccccggcca ccaggccgag gaccacgccg ccggagaccg ccaccccggc
     1861 catcacgcca cgcagaacgg gccctcgggc cgctgccgca gggtgttcgt gccgcgcggc
     1921 ggcccggtct ggagcttgcc ggtgtcgacc atcacggtgt cgccgtcgag gacgatcggg
     1981 aagcggtcca ggctccgggg cgccgggccg agctggtact cgccggcccg gttgtacttc
     2041 gacccgtggc aggggcactc gaaccactgg ctctgctcgc agaacgggac gcggcagccg
     2101 aggtgcacgc agcgctggta gagcgccatc agcccgccgg cggcgatgcc ctcgtactcc
     2161 gtcgggaccg ggtcgacgtc gtactgcacg atgtagaagc gcccggcgcc gaggtagaac
     2221 ggcgcgcggc tgtccgagat ctggttcttg atctcctgga ccgtcccggc cgcgatcacc
     2281 gacccgaacc cgcccctcag gttcgggtac aggaatgcca ggctcgcgcc gccgaactgc
     2341 gccaggaaga cgagcatcga gccgagcagc gagccgcgga agaagtcccg ccgcgagatc
     2401 ggtttcgggg cggcggccgg gggcggctcg gccgggcgcc cggcccgctc cagcgccccg
     2461 gcccggcgcg cccggaggaa cgacgccgcg agcagcagca ggaacagccc gaacagcgcg
     2521 ccgaccagga gcaggccgaa cgtctgcgat gagatcccca ggccgctcac agctcgaaga
     2581 acaggccgcc ctgccaggga aagacgaagt tgtagcccgg gccgcggaag aaggacccga
     2641 tgatcaccag gatcgaccag aacaggatga acagcgtgaa gatcgtgatc gccagcttgc
     2701 ggtcgccggg cttcgtgctc gggttacggt ccaggtacgg gaccacgatc agcccgagga
     2761 tggcgagcgt cggcatcagc acgccggcga ccatcgggtg gaagtagcgc agcagctcct
     2821 gcaggccgag gaagtaccac ggcgctttcg aggggttggg ggtcaggttc gggttggcca
     2881 gctcgcgcag cggcgcgttg acgaacgtgg agaacacgat caggaacgcg gtgaccacga
     2941 gcaggctgac gaactcctcg atcagcaggt g
//
LOCUS       DASPWO010000329         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_219728, whole genome shotgun sequence.
ACCESSION   DASPWO010000329 DASPWO010000000
VERSION     DASPWO010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219728"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VEO00_07565"
     CDS             <1..294
                     /locus_tag="VEO00_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYU57891.1"
                     /translation="SGFAFPEILEAIRASHERADADRAGHLFDRFLPLIAFEAQAGVG
                     VAARKELLRRRGAIGTAVTRQPLRTLDAVLRAELDAVLARVGVDPGPDRLAID"
     gene            355..588
                     /locus_tag="VEO00_07570"
     CDS             355..588
                     /locus_tag="VEO00_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYU57892.1"
                     /translation="MSEATKLAVQAASRDPRVGLRGVSALRRLLEQLEALQVHNARAS
                     GWSWQEIADHLGVSKQAAHKKHAGRRRRRLRSS"
     gene            588..1151
                     /locus_tag="VEO00_07575"
     CDS             588..1151
                     /locus_tag="VEO00_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HYU57893.1"
                     /translation="MFERFDPGARHIVLRAREEAARLRHGYLGKEHLLLVLADERHTV
                     AGSVLRDLGVTPEGVEREIVRIVGWGPPPRFGDDDADALRALGIDLQEVRRVIEGAFG
                     PGSLDRAPEGCAAPGSFSFTRKSKEALQLAVREARHLGHASIRPEHLLLGLVRSEGGV
                     GGEVLAALGVAPRVVRARVLERLAIAS"
     gene            1195..1998
                     /locus_tag="VEO00_07580"
     CDS             1195..1998
                     /locus_tag="VEO00_07580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYU57894.1"
                     /translation="MGDEDHGRALAARYSRNAAHYRRYWAALTTPRGRVLLERLPLDG
                     ARRLLDLGTGVGGLLPTMAAAAPGATVVGVDRAEGMLRAAPAGFPRAVMDAQRVAFRD
                     GVFDAVIMAFVLFHLPDPQAGLRSAHRVLRPGGWFALSTWRDPPGDSPANRIWGEELT
                     RAGAPPDDPAPSRHDLMDTPEKLTRLLHAAGFEDVRTDGHVLDDVMDLDEFLDRRTGL
                     GLSADRFLSLSGPAREACLVSARRRLSKLATEDFVIREDAILAWARRPE"
     gene            complement(2007..>2349)
                     /locus_tag="VEO00_07585"
     CDS             complement(2007..>2349)
                     /locus_tag="VEO00_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HYU57895.1"
                     /translation="DEAHGVERSIGRVFRQGIVVNALNPKTAVFFLAFLPQFIDPSRG
                     APAAQTAILGLTFIGLGMCTDSVYALAASAARRVLRRSRRFAKAQRYTAGTVFVGLGV
                     AAAVTGSRSHA"
BASE COUNT          303 a          824 c          903 g          319 t
ORIGIN      
        1 tccgggttcg cctttccgga gatcctggag gcgatccgcg cctcccacga gcgggccgac
       61 gcggaccgcg ccggccacct gttcgaccgc ttcctgccgc tgatcgcctt cgaggcgcaa
      121 gcgggagtcg gggtcgccgc ccgcaaggag ctgctccggc gccgcggggc gatcggcacc
      181 gcggtcaccc ggcagccgct gcgaacgctc gatgccgtgc tgcgggccga gctcgacgcc
      241 gtcctggccc gggtgggcgt cgaccccgga ccggaccggt tggcgatcga ctgagcgcgt
      301 cgttcgggtc aaccggagtt gacacgagcg cggccgtcaa cgtaggatga cggcatgtcc
      361 gaggcgacga agttggcggt gcaagccgcg agccgggatc ctcgtgtggg cctgagggga
      421 gtgtcggccc tgcgacggct gctcgaacaa ctcgaggccc tgcaggtcca caacgcccga
      481 gcgtccgggt ggtcctggca ggagatcgcc gaccacctcg gggtcagcaa gcaggcggcg
      541 cacaagaagc atgccggacg gcgccggcgg cggctgcggt cgtcctgatg ttcgagcggt
      601 tcgatcccgg ggcgcggcac atcgtcctcc gtgctcgcga ggaggccgcc aggctgcggc
      661 acggctacct ggggaaggag cacctcctgc tcgtcctggc agatgagcga cacacggtcg
      721 cgggcagcgt gcttcgagac ctgggagtga cgcccgaagg cgtggagcgg gagatcgttc
      781 ggatcgtcgg ctggggaccg ccacctcgat tcggggacga tgacgcggac gccctgcggg
      841 cgctggggat cgacctccag gaggttcgcc gggtcatcga gggagcgttc ggcccgggct
      901 cgctcgatcg cgcgccggaa gggtgcgccg cgcctggctc gttctccttc acgcggaagt
      961 cgaaggaggc gctgcagctc gcggtcagag aggcgagaca cctcggacac gcgtccatcc
     1021 gcccggagca cctcctcctc gggctcgtcc ggtcggaggg cggcgtcggc ggtgaggtgc
     1081 tcgccgccct cggcgtggcg ccgcgcgtcg ttcgggcgcg ggtgctggag cgcctggcca
     1141 tcgccagctg agcccgctgg ccccgggcga gtccggggtg ccatcctccc ttccatgggg
     1201 gatgaggacc atggccgcgc gctggccgct cgctactcgc ggaacgccgc gcactaccgg
     1261 cggtactggg ccgcgctgac gacgccccgg ggccgggtgc tgctggagcg gctgcccctc
     1321 gacggcgccc gccggctgct ggacctcggg accggagtgg gcggcctgct ccccaccatg
     1381 gcggcggccg cgcccggcgc gacggtggtg ggggtggacc gcgccgaggg gatgctgcgc
     1441 gccgcgccgg ccggattccc gcgcgcggtg atggacgcac agcgggtcgc gttccgcgac
     1501 ggcgtgttcg acgcggtgat catggcgttc gtgctcttcc acctcccgga ccctcaggcc
     1561 ggactgcgtt cggcgcaccg cgtcctgcgg cccggcggct ggttcgcgct gtccacgtgg
     1621 cgagatcccc cgggggattc cccggcgaac cggatctggg gcgaggagct gactcgggcg
     1681 ggcgcgccgc ccgacgaccc ggcgccctcg cggcatgacc tgatggacac gccagagaag
     1741 ctcacccggc tgctgcacgc cgccgggttc gaggacgtcc gcaccgacgg tcacgtgctg
     1801 gacgacgtga tggacctcga tgagttcctg gaccggcgca ccgggctcgg cctctcggca
     1861 gaccgattcc tgtcgctctc cgggcccgcc cgggaggcct gcctcgtcag cgcccgccgc
     1921 cgactgtcca agctcgccac ggaggacttc gtcatccgcg aggatgccat cctcgcctgg
     1981 gcccgacgcc cggagtgacg agggcctcag gcgtgcgagc gggagccggt gacggcggcg
     2041 gccacgccga gaccgacgaa cacggtgccc gccgtgtagc gctgggcctt cgcgaagcgg
     2101 cggctgcggc gcagcacgcg gcgggccgcc gaggcggcga gcgcgtagac gctgtcggtg
     2161 cacatgccca gcccgatgaa cgtgagaccg aggatcgcgg tctgcgcggc gggggcgccg
     2221 cgcgacgggt cgatgaactg cggcaggaag gccaggaaga agacggccgt cttggggttc
     2281 agcgcgttca ccacgatgcc ctgacggaac acgcggccga tggaccgctc cacgccgtgg
     2341 gcctcgtcg
//
LOCUS       DASPWO010000330         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_817096, whole genome shotgun sequence.
ACCESSION   DASPWO010000330 DASPWO010000000
VERSION     DASPWO010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817096"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1597)
                     /locus_tag="VEO00_07590"
     CDS             complement(<1..1597)
                     /locus_tag="VEO00_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HYU57896.1"
                     /translation="MAEDLQTKILPLLPLTTGVVLPGMVVTLTLESDEARAAIFAAQG
                     ADGDVLLVPRRENGRYASVGTVASIEEVGRTPSGVEALVVRGTHRAVIGRGVPGTGDA
                     TWVEIEPHPDPDEATPRAKELAREYRATLENIIEERGLPAQIVEFLRGISDPGAIADT
                     SGYSPDLSFEQKIEVLETLDVEARLEKVLGWAKDALAEATLKGKIAEDVREGMENTQR
                     EFILRQQMAAIRKELGEDSEENVVEEYRTKIEEANMPEGPKAEALRELGRLERTSEQS
                     PEYGWIRTYLDWMTELPWDVRTEDNLDITDARRILDEDHTGLEDVKDRIVEYLAVRKL
                     RQERGLGAVHGRGSGAILTLIGPPGVGKTSLGESVARALGRKFVRVSLGGIHDEAEIR
                     GHRRTYVGALPGRIVRALKEAGTKNPVFMLDEIDKVGADWRGDPSSALLEVLDPAQNH
                     SFRDHYLEVDLDLSEVLFIATGNVAETIPGALLDRMEVIRLDGYTEDEKVAIARDHLL
                     GRQLERNGLTEGEVSLTDEALHAI"
     gene            complement(1750..2106)
                     /locus_tag="VEO00_07595"
     CDS             complement(1750..2106)
                     /locus_tag="VEO00_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57897.1"
                     /translation="MDSGGAAWTFVMVTDRRLRWVPDARPRFEAALDLDGVTAVSQRM
                     KTHRYAIALEHAPLRRLHHVPAHRLLMFAWGNAVAEDLFTRTELASSRRDTAAARALR
                     EQLSLRGALDATARPI"
     gene            complement(2478..>2873)
                     /locus_tag="VEO00_07600"
     CDS             complement(2478..>2873)
                     /locus_tag="VEO00_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HYU57898.1"
                     /translation="VIGPTGMFVLETKDWQRRFYPSAGRLFHNGRDAHDAVTQAQRAA
                     MEVKQRPAAAGLPRVWVEAWVVSTRATIQGAKGGVLTLDRVTVRQLPGLADAVRAGNG
                     RLTPVEVARARAAVLRWDRGPVTVRSLSG"
BASE COUNT          390 a         1037 c         1002 g          444 t
ORIGIN      
        1 tgatggcgtg gagcgcctcg tcggtcagcg agacctcccc ctccgtgagg ccgttgcgct
       61 cgagctgtcg ccccagcagg tggtcccgag cgatggcgac cttctcgtcc tcggtgtagc
      121 cgtcgaggcg gatgacctcc atccggtcga gcagcgctcc ggggatcgtc tcggcaacgt
      181 tcccggtggc gatgaacagg acctcggaca ggtccaggtc gacctccagg tagtggtcgc
      241 ggaacgagtg gttctgcgcc gggtcgagca cctcaagcag cgctgacgac gggtcgccgc
      301 gccagtccgc gccgaccttg tcgatctcgt cgagcatgaa gaccgggttc ttcgtgccgg
      361 cctccttcag cgcgcggacg atccgcccgg gcagcgcgcc gacgtaggtg cggcggtgtc
      421 cgcggatctc ggcctcgtcg tggatgccgc cgagcgacac gcgcacgaac ttgcgcccga
      481 gcgcgcgcgc cacggactcg ccgagcgacg tcttgccgac gcccggcggg ccgatcagcg
      541 tcaggatcgc gccggagccg cggccgtgga ccgcgccgag cccgcgctcc tgccggagct
      601 tgcgcacggc gaggtactcg acgatgcggt ccttgacgtc ctccaggccg gtgtggtcct
      661 cgtccaggat gcgccgcgcg tcggtgatgt cgaggttgtc ctcggtgcgg acgtcccacg
      721 gcagctcggt catccagtcc aggtaggtcc ggatccagcc gtactcgggc gactgctcgg
      781 aggtgcgctc cagccggccc agctcgcgca gggcctcggc cttcgggcct tccggcatgt
      841 tcgcctcttc gatcttggtc cggtactcct cgacgacgtt ctcctcggag tcctcgccga
      901 gctccttgcg gatcgcggcc atctgctggc gcaggatgaa ctcgcgctgg gtgttctcca
      961 tgccctcgcg gacgtcctcg gcgatcttgc ccttcagggt ggcctcggcc agggcgtcct
     1021 tcgcccagcc gagcaccttc tccaggcgcg cctcgacgtc gagggtctcg aggacctcga
     1081 tcttctgctc gaaggagagg tccggcgagt agcccgacgt gtcggcgatg gcgccggggt
     1141 cggagatccc gcgcaggaac tcgacgatct gggccggcag gccccgctcc tcgatgatgt
     1201 tctcgagggt cgcccggtac tcgcgggcga gctccttggc tctcggggtg gcctcgtccg
     1261 ggtccgggtg gggctcgatc tcgacccagg tcgcgtcccc ggtcccggga accccccgcc
     1321 cgatcacggc ccggtgggtc ccacgaacga cgagcgcctc gacccccgag ggggtccgtc
     1381 cgacctcctc gatcgaggcc acggttccca cggaagcata gcgaccattc tcgcgccgag
     1441 gcacgagcaa gacgtcccca tcagccccct gagcagcgaa aatagccgcc cgagcctcat
     1501 ccgactcgag cgtgagcgtg accaccatcc ccggcagaac cacgccggtg gtcagcggaa
     1561 gaagcggcag aatcttggtt tgcaggtcct cagccatgcc atctccagag tagttgcaga
     1621 tgcaagaacg ctcgcccccc cgcattcatt ccaaacctgg gccagggcta gccttcgccg
     1681 agcctggcgg cggcgcggct cgcgcgcatt ccggcgtaac gccgtcccgt gccgacggat
     1741 gtggggctcc tagatcggcc gagcggtcgc gtccagcgcg ccgcggaggc tcagttgctc
     1801 ccggagggcc cgggcggcgg cggtgtcgcg gcggctggag gcgagctccg ttcgggtgaa
     1861 gaggtcctcc gcgacggcgt tgccccacgc gaacatcagg agccggtggg ccggcacgtg
     1921 gtggagccgg cgcagcggcg catgctccag tgcgatcgcg taccggtgcg tcttcatccg
     1981 ctgggacacc gcggtcacgc catcgaggtc gagcgcggcc tcgaacctcg gcctggcatc
     2041 gggaacccag cgcagccgcc ggtcggtgac catgacgaag gtccatgcgg cgccaccgct
     2101 gtccaccccc gccgcgctcg gtgatgtccg cgcagcggcc gaccgccagg acccgctccc
     2161 ccgggtcgag atggctccgc acgcgcgcgt cgcagtcgcg cagcatgtcg tcgaggctcg
     2221 gcaccccatc agcgtaggcg gctcccacga ggtctgggga ccggcccttc gggcgaggtc
     2281 ggtgggatcg agtggccgat gggggcagat gccccccgct cctggaggcg cccgaggtgc
     2341 gtggggcgtt cgatccgctc ggagacggac gacaacgacc gcgcgggcgc tggtacgaga
     2401 ggcggtgtgt cagcgtcaac cgaacggtcg cacgtcccga gtatcccgcc gcgcgccggt
     2461 ccgggcaagc tcgctgatca gccgctcagc gagcggacgg tgaccgggcc gcggtcccat
     2521 cggagcacgg cggcccgggc tcgggcgacc tcgactgggg tcagtctccc gtttccagcg
     2581 cggacagcgt cggcgaggcc gggtagctgg cggaccgtca cccgatcgag cgtcagcact
     2641 ccgcccttcg cgccctggat ggtcgcccgc gtcgagacca cccacgcctc gacccagacc
     2701 cgcggcaggc cggcggccgc cgggcgctgc ttgacctcca tggcggcccg ctgagcctgg
     2761 gtcacggcat cgtgcgcatc gcggccgttg tggaacagcc ggccggcgct cggatagaac
     2821 cgtctctgcc aatccttcgt ctccaacacg aacatgccgg tcggcccgat gac
//
LOCUS       DASPWO010000331         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_898202, whole genome shotgun sequence.
ACCESSION   DASPWO010000331 DASPWO010000000
VERSION     DASPWO010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898202"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VEO00_07605"
     CDS             <1..1324
                     /locus_tag="VEO00_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYU57899.1"
                     /translation="SIREKTILSDLTAAGKRIVFTDVPLTFPPPKVEGAVIAGGVLPK
                     RRPFTHPLDLAGELEAAGVPWPINGMSWTTFHNRPLPLLDEAEQITAARQRVNERLLD
                     GDDWDVACAVYVATDRIQHCLSNHISPDHPDYAERSKTRVAARIREIYRQLDEGLGAL
                     VAKARGDDLVIFMSDHGFHSATRTMHMDRFLEHHGLLQFSASQAVVGRLQAGKLRAVA
                     RRVYDLFGLHGKVSLPQPVNWSKTRAYTSIRSTGEGVSVNLAGREPEGIVDPADYDRV
                     RDAVAAAIEGFVDPATGRKPVARVRTREEVFAGRHLERAPDLLLEPSPGFSLTHAKQM
                     IEDAGWATGDHRVEGVIVAAGPRVRADAFAEDPLLIDLAPTILAAVDAPASIKHDGQV
                     LSAVIGDEAAVRAASISAADVGVEGDAGPDEDEALEMEEHLRGLGYLE"
     gene            1334..2908
                     /locus_tag="VEO00_07610"
     CDS             1334..2908
                     /locus_tag="VEO00_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HYU57900.1"
                     /translation="MDAAGPPPTDAVPPTTPASEDRADRLTILSGTGQNVAGLVVALL
                     ATFGSQVLITRSLGAAAYGVVYVGTQVAFVGAAATRFGMDVAAVRRVAIDVGKGDPGR
                     ARAVVSRAAAIATVASVVSAVALYLAAPALAGSGGIPANAVGVFRAAALALPFVALAQ
                     VYLGGTRGLKIMSHTLSIYWAGQPLAWIALMAAGFAFRPTETTGVLAYAGSWILATLA
                     ALWAWRHETRAFGHLPPAAGETADLLRYGTPRAPASLFAQLLFVADLFVLARYAPASE
                     YGVYAAAVRVGQVVVLFLISVNLVFSPFVADLHARGRHDRLNDLFKAVTRWTFAATLP
                     VILTLSVLAEPVLRLFGGEFATAGARTALTVLIAGQAVNVAVGSVGFILIMVGRTGWD
                     LVVYTASIALDLALAFWLAPRYGMEGAAVAQAVTLVASNALRLYLVWRFLRIQPFSRD
                     YARLALPAIAGALAMIAVHLPLADGPWPVDLLVSAFAGTLAYTAALIVFGLAPEERAA
                     AGRLLRNLRAGGLRTR"
     gene            3136..>3636
                     /locus_tag="VEO00_07615"
     CDS             3136..>3636
                     /locus_tag="VEO00_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57901.1"
                     /translation="MTKYVQAMVTGIGTGAVYALVAYGISLVYAVTRTVNLAHGEVMM
                     TAVFASLTALTYGAAAWLAIAAGLGAAALLGLVLDRVVMTPLAGRREPMSWFLGVVMF
                     AAVLRGAATLLFGSRTYPLPIDIGGDAIIRLGGAAFKATYVWILGLALVLAAAFQVLV
                     ARTAFGR"
BASE COUNT          420 a         1346 c         1332 g          538 t
ORIGIN      
        1 ctcgatccgg gagaagacga tcctgtccga cctcaccgcg gccgggaagc ggatcgtctt
       61 caccgacgtc ccgttgacgt tccccccgcc gaaggtcgag ggcgcggtga tcgccggcgg
      121 cgtgctcccg aagcggcgcc cgttcacgca cccgctggac ctggccggcg agctggaggc
      181 ggccggcgtc ccctggccga tcaacgggat gtcgtggacc accttccaca accgcccgct
      241 cccgctgctc gacgaggccg agcagatcac ggccgcccgc cagagggtca acgagcgcct
      301 gctcgacggg gacgactggg acgtggcctg cgccgtgtac gtcgccaccg accgcatcca
      361 gcactgcctg tcgaaccaca tctcgcccga ccacccggac tacgccgagc gctcgaagac
      421 gcgggtcgcg gcgcggatcc gcgagatcta ccgccagctc gacgaggggc tcggcgcgct
      481 ggtcgcgaag gcccggggcg acgacctggt gatcttcatg agcgaccacg ggttccactc
      541 cgcgacgcgg acgatgcaca tggaccggtt cctggagcac cacgggctcc tgcagttctc
      601 ggcgagccag gccgtcgtcg ggcggctgca ggccgggaag ctccgcgccg tggcgcgccg
      661 cgtctacgac ctgttcggcc tccacggcaa ggtgtcgctg ccgcagccgg tgaactggtc
      721 gaagacgcgg gcgtacacgt cgatccgctc gacgggcgag ggcgtcagcg tgaacctcgc
      781 cggtcgcgag cccgagggga tcgtcgaccc ggccgactac gaccgggtgc gggacgcggt
      841 cgccgccgcg atcgaggggt tcgtcgaccc ggccaccggc cgcaagccgg tggcccgcgt
      901 gcgaacgcgc gaggaggtct tcgccgggag gcacctcgag cgggcgccgg acctgctgct
      961 cgagccctcg ccggggttca gcctgaccca cgccaagcag atgatcgagg atgccggctg
     1021 ggccaccggc gaccatcgcg tcgagggcgt gatcgtggcg gctggcccgc gcgtgcgggc
     1081 ggacgcgttc gccgaggacc cgctgctgat cgacctggcg ccgacgatcc tcgccgcggt
     1141 cgacgccccc gcctcgatca agcacgacgg gcaggtgctc tcggcggtga tcggcgacga
     1201 ggccgcggtc cgggccgcct cgatctcggc ggcggacgtc ggcgtggagg gcgacgccgg
     1261 gcccgacgag gacgaggcgc tcgagatgga ggagcacctc cgcggcctgg gatacctgga
     1321 gtagccgggc ccggtggacg ccgcgggccc acctcccacc gacgccgtcc ccccgacgac
     1381 ccccgcgtcc gaggaccgcg ccgaccggct gacgatcctc tccgggacgg ggcagaacgt
     1441 cgccggcctg gtcgtcgcgc tcctggcgac gttcggctcc caggtgctga tcacgcgctc
     1501 gctgggcgcg gccgcctacg gcgtggtgta cgtcgggacg caggtggcgt tcgtcggggc
     1561 cgcggcgacg cggttcggca tggacgtcgc cgcggtgcgt cgcgtcgcga tcgacgtcgg
     1621 gaagggcgac ccgggccgtg cccgcgcggt ggtctcccgc gccgcggcca tcgccacggt
     1681 cgcgtcggtc gtgtccgcgg tcgcgctgta cctggccgcg cccgcgctcg ccgggtccgg
     1741 cggcatcccg gcgaacgcgg tcggcgtctt ccgcgcggcc gcgctggcgc tcccgttcgt
     1801 ggcgctggcc caggtctacc tcggcgggac gcgcgggctg aagatcatga gccacacgct
     1861 gtcgatctac tgggccggac agccgctggc ctggatcgcg ctgatggcgg ccggcttcgc
     1921 gttccgcccg accgagacga ccggcgtcct ggcctacgcc ggttcgtgga tcctggcgac
     1981 cctcgccgcg ctgtgggcct ggcggcacga gacccgcgcg ttcggccacc tgccgccggc
     2041 cgcgggggag accgccgacc tgctccgcta cggcacgccg cgcgcgcccg cgtcgctgtt
     2101 cgcgcagctg ctgttcgtcg ccgacctgtt cgtgctggcc cgctacgcgc ctgcttcgga
     2161 gtacggcgtc tacgcggccg ccgtacgcgt cggccaggtg gtggtgctgt tcctgatctc
     2221 ggtgaacctg gtgttcagcc ccttcgtcgc cgacctgcac gcgcgcggcc ggcacgaccg
     2281 gctgaacgac ctcttcaagg cggtcacgcg ctggacgttc gccgccacgc tcccggtgat
     2341 cctgacgctc tccgtcctgg ccgagccggt gctgcggctg ttcggcggcg agttcgccac
     2401 cgccggcgcg cgcaccgcgc tgacggtcct gatcgccgga caggccgtga acgtcgccgt
     2461 cgggagcgtc ggcttcatcc tgatcatggt cgggcgcacc ggctgggacc tggtcgtcta
     2521 cacggcctcg atcgcgctcg acctggcgct cgcgttctgg ctcgcgccgc gctacgggat
     2581 ggagggcgcg gcggtggcgc aggcggtcac gctcgtcgcc tccaacgccc tgcgcctcta
     2641 cctcgtctgg cgtttcctgc gcatccagcc cttcagtcgc gactatgccc gtctggcgct
     2701 cccggcgatc gccggtgcgc tggcgatgat cgccgtccac ctcccgctgg ccgacggccc
     2761 gtggccggtg gacctcctgg tcagcgcctt cgccgggacc ctggcctaca cggccgcgct
     2821 catcgtcttc ggcctggccc cagaggaacg cgcggcggcg ggccggctgc tgcggaacct
     2881 gcgggccggc ggcctccgga cgcgataggc gcggatattc ctacgtgccg caggagttcc
     2941 tgcggtcgag acgcggccgg cgcatccagc gggacgtcgt gggaccaggt gctcgcgacc
     3001 cgagcacgtg gagttcttcg ccgcctaccg ccacctcgcc cgccgcctgg caggctgtag
     3061 ggggtctggg ggtagccgcc ccgtgcggga tcgaacgaac gttgcccggc gggcgaacct
     3121 cccagggagg acgcggtgac gaagtacgtc caggccatgg tcacggggat cgggacgggg
     3181 gccgtctacg cgctggtcgc ctacgggatc tcgctcgtct acgccgtgac ccggaccgtg
     3241 aacctggcgc acggcgaggt gatgatgacc gccgtgttcg cctcgctcac ggcgctgacg
     3301 tacggcgccg ccgcgtggct ggcgatcgcc gccggcctcg gcgccgccgc gctcctcggc
     3361 ctggtcctcg accgggtggt gatgaccccg ctcgccgggc gacgcgagcc gatgtcgtgg
     3421 ttcctcggcg tggtgatgtt cgcggccgtc ctgcgcgggg cggcgaccct gctgttcggc
     3481 tcgcggacct acccgctgcc gatcgacatc ggcggggacg cgatcatccg cctcggcggc
     3541 gcggccttca aggccaccta cgtctggatc ctggggctgg cactcgtgct ggccgcggcg
     3601 ttccaggtgc tcgttgccag gacggcgttc gggcgc
//
LOCUS       DASPWO010000332         2814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1150343, whole genome shotgun sequence.
ACCESSION   DASPWO010000332 DASPWO010000000
VERSION     DASPWO010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150343"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VEO00_07620"
     CDS             <1..586
                     /locus_tag="VEO00_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HYU57902.1"
                     /translation="QVPADPDAEDRGGARLADALVALATASREAEPAPPTLVVHADAA
                     VLAGSEPESGPVLAESEDGGRLAAETVRRLACVAWIEWVLHAQGGPVGIGRRGRQVRG
                     TVARLLRHRDLTCRFPGLRPPPLPARPSPGALGPRGPTDLDNLILLCGHHHRLLHEGG
                     WSISGHPGRHLRFHDPTGRVVPSRAPVRDMVVQC"
     gene            complement(629..2062)
                     /gene="gatB"
                     /locus_tag="VEO00_07625"
     CDS             complement(629..2062)
                     /gene="gatB"
                     /locus_tag="VEO00_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HYU57903.1"
                     /translation="MTYEPVIGLECHVELATRTKMFCGCANSFGAAPNTQVCEVCLGH
                     PGTLPVANGRAVEFTMKIGLALGCEIAPRSLFHRKNYFYPDMPKNYQISQFDLPLCTG
                     GALEIEVGGETRRIGVHRVHLEEDTGKTLHMGGASGRIADANYALEDYNRAGVPLVEV
                     VSEPDMRSADEARAYFQELRLILESLGVSDVRMEEGSLRCDANVSVRPQGATDLGTKV
                     EIKNLNSVRSLERAIRFEAERQRAALERGEELVQETRHWDEGAGLTLPLRSKEYAFDY
                     RYFPEPDLQPIEPGADWIDRLRSELPELPAARRARLSDRFQLSPEQLVVIGSSSETAR
                     LFEEAFNRLEGVGPRTIANWIVNVGPDLVRDELDALVDVIRLTELGEISVLAGKEVLA
                     EALATGQDPKDIVEAKGLRQISDEGALTAVIDRVIAENPGPVEQFRAGKEGVLGFLVG
                     QVMKATNGSANPKEAGRLLRERLMPQG"
     gene            complement(2059..2733)
                     /locus_tag="VEO00_07630"
     CDS             complement(2059..2733)
                     /locus_tag="VEO00_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57904.1"
                     /translation="MSESASGSRPGGVGLVRWASLAGLAVAVFLGVFSFDRGAPEAWS
                     ESVGLASLVAGPFGLALLATRLRGPGRREAVWLAAGVLALVIGVLSFSAVVVIAPPAV
                     VILIGAVRELIAFGDRPRGTLVVLFAFVLLAGAGAGMALFLRTDPRCHAIGTTGVSCT
                     SDAITWIETALSISVWAFAFAVITAALAGDRREQLGRRPEPIRPRTPAAGEGNGKSAA
                     AKERGA"
BASE COUNT          412 a         1041 c          975 g          386 t
ORIGIN      
        1 gcaggtgccg gccgacccgg acgccgagga ccgcggcggc gcgaggttgg ccgacgcgct
       61 cgtggcgctg gccaccgcct ctcgtgaggc cgagccggcc ccgccgaccc tggtggtcca
      121 cgccgacgcc gccgtgctcg cgggatcgga gccggagagc ggcccggtgc tcgccgagag
      181 cgaggacggg gggcgcctgg cggccgagac cgtccggcgc ctggcctgtg tcgcctggat
      241 cgagtgggtg ctccacgcgc agggcggccc ggtcgggatc gggcggcgcg ggcggcaggt
      301 ccgggggacc gtggcccggc tgctgcggca ccgcgacctc acctgccgct tccccgggct
      361 gcgcccaccg ccgcttcctg cacgcccatc acctggtgca ctgggcccgc ggggccccac
      421 cgaccttgac aacctgatcc tgctgtgcgg acaccaccac cgcctgctgc acgagggagg
      481 ctggtcgatc agcggccacc ccggacgcca cctgcggttc cacgacccca cggggagggt
      541 ggtgccctcg cgggcgcccg tacgggacat ggtcgtgcag tgctgaggtc aagcggtctc
      601 gaggtcgtgg cgctgccggc gcgtgcccct acccctgggg catcaagcgc tcgcgcagga
      661 ggcggccggc ctccttcggg ttggcggagc cgttcgtcgc cttcatcacc tggccgacca
      721 ggaagccgag cacgccctcc ttccccgcgc ggaactgctc caccgggccg gggttctcgg
      781 cgatgacccg gtcgatcacg gcggtgagcg caccctcgtc ggagatctgg cgcagaccct
      841 tcgcctcgac gatgtctttc gggtcctggc ccgtggctag ggcctcagca agaacctcct
      901 tgccggccag caccgatatc tcccccagct cggtgagacg aatcacgtcg accaaggcgt
      961 cgagctcgtc tcgaacgagg tcgggcccaa cattcacgat ccaattggcg atcgtccttg
     1021 gaccgacacc ttcgagacgg ttgaacgcct cctcgaacag cctcgccgtc tcgctcgacg
     1081 acccaatgac cacaagctgt tcagggctga gttggaaccg gtcgctcaag cgggctcgcc
     1141 gggcggcggg gagctcgggc agctccgagc gaaggcgatc gatccagtcg gcgccgggct
     1201 cgatcggctg caggtcaggc tccgggaagt agcggtagtc gaacgcgtac tccttcgagc
     1261 gcagcggcag cgtcaggccg gcgccctcgt cccagtggcg cgtctcctgc acgagctctt
     1321 cgccgcgctc cagcgccgcc cgctggcgct cggcctcgaa ccggatcgcc cgttccagcg
     1381 agcgcaccga gttgaggttc ttgatctcca ccttggtccc gagatcggtg gcgccctgcg
     1441 gacggaccga cacgttcgcg tcgcagcgca gtgacccctc ctccatccgc acgtccgaca
     1501 cgccgagcga ttcgaggatc agccggagct cctggaagta cgcgcgcgcc tcgtcggccg
     1561 agcgcatgtc gggctcggag accacctcca cgagcggcac accggcgcgg ttgtagtcct
     1621 ccagcgcgta gttcgcgtcg gcgatgcgtc cgctggcccc gcccatgtgc agggtcttcc
     1681 cggtgtcctc ctcgaggtgg acgcggtgca ccccgatccg gcgcgtctcg ccgccgacct
     1741 cgatctcgag cgcgccgccg gtgcacagcg gcaggtcgaa ctgggagatc tgatagttct
     1801 tcggcatgtc gggatagaag tagttcttgc gatggaacag cgagcgcggc gcgatctcgc
     1861 agccgagcgc cagcccgatc ttcatcgtga actccaccgc gcggccgttg gccacgggga
     1921 gcgtcccggg gtggccgagg cacacctcgc agacctgcgt gttcggtgcc gccccgaacg
     1981 agttcgcgca gccgcagaac atcttcgtcc gcgtcgccag ctcgacgtgg cactccaggc
     2041 cgatgacggg ttcgtaggtc acgccccgcg ctccttcgcc gcagccgact tcccgttgcc
     2101 ctcgccggcg gcgggagttc gcgggcggat cggttccggc cgccgcccga gctgctcccg
     2161 gcggtcaccg gcgagggccg ccgtgatcac cgcgaaggcg aacgcccaca cggagatcga
     2221 cagcgcggtc tcgatccagg tgatggcgtc ggacgtgcac gagacgccgg tcgtccctat
     2281 cgcgtgacat cgagggtccg tccgtaggaa caacgccatc cctgcgcccg cgcccgccag
     2341 cagcacgaag gcaaacagga cgaccagggt tccccgggga cggtcgccga aggcgatcag
     2401 ctctcgaacg gctccgatca ggatcacgac cgccggcgga gcgatcacca cgacggcgga
     2461 gaacgagagc acgccgatga cgagcgcgag cacgccggcc gccagccaca cggcctcgcg
     2521 gcggccgggc ccgcgcaacc gggtggcgag cagtgccagc ccgaatggcc cggcgacgag
     2581 cgaagcgagc ccgaccgact ccgaccacgc ttccggcgcg ccgcgatcga agctgaagac
     2641 gcccaggaag acggcgaccg ccaggcctgc cagcgatgcc caccggacga ggcccacgcc
     2701 gcccggccgc gagcccgagg ccgattcgct cacgcggcct cctcgagcat gggcgggcgc
     2761 gcatcgaagc cgaggtcctg ctcgaaggcg tgggcggcgc gcaggaccgt cgcc
//
LOCUS       DASPWO010000333         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_817478, whole genome shotgun sequence.
ACCESSION   DASPWO010000333 DASPWO010000000
VERSION     DASPWO010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817478"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..651
                     /locus_tag="VEO00_07635"
     CDS             106..651
                     /locus_tag="VEO00_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HYU57905.1"
                     /translation="MGSDLRERKWSRFFTLLDRDGDGLLERSDFEAIVDGVAGARGIE
                     RGTPQYDMLHAAVMLTWSHIEEFADQNRDGKATREEWFQTLEDTIRDEAQYAKYVTPF
                     AEGVFDLLDEDGDGVVGEDEYVRFMRIFGGTEENGREAFRHVDADGDGRLTRDELLER
                     VREWETGEAPAAPGNWLFGEF"
     gene            complement(734..>2202)
                     /locus_tag="VEO00_07640"
     CDS             complement(734..>2202)
                     /locus_tag="VEO00_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYU57906.1"
                     /translation="FTRGIHPTMYRGRLWTMRQYAGMGTAEETNRRFRYLLDRGQTGL
                     SVAFDLPTQMGLDSDHPRAEGEVGRTGVAIDSVEDMHRLLDGIPLDRVTTSMTINATA
                     AVLLLLYEIVAQERGIEPAALGGTVQNDILKEYAARGTYVYPPGPSMRLITDLFAYAR
                     ERLPRWNTISISGYHMREAGSTAVQEVAFTLANAIAYVEAALAAGLHIDDFGPRLSFF
                     FACHMNFFEEAAKFRAARRLWSRIARARFGARDPRSAMLRFHTQTGGATLTAQQPENN
                     IVRTALEALSAVLGGTQSLHTNSFDEALALPTEHAVTVALRTQQVIAFESGVTDTVDP
                     LAGAYFVESLTDEIEARAEEYLEKIDGMGGAVAAIEAGWIQDEIAEAAYRIQMGVESG
                     ERVVVGVNRYAEEADVPPDLLHLDEAAITAQVERVRRLRAERDGRAVDAALASVEETA
                     GGTGNLLLPMREALAARATLGEVSDALRRVFGEYRPSR"
BASE COUNT          314 a          785 c          790 g          313 t
ORIGIN      
        1 gggccagtcg cagcgcaatc ccgaacccct cgtcgccggt ccccccgacg atcgccaccg
       61 ccacggcgcg caggatacgc tgcccaccaa tagggagggg atccgatggg gagcgacctg
      121 cgagagagga agtggagccg gttcttcacc ctgctcgacc gcgacgggga cgggctgctc
      181 gagcgctccg acttcgaggc gatcgtggac ggcgtcgccg gcgcccgcgg catcgagcgc
      241 ggcacgccgc agtacgacat gctccacgcg gcggtgatgc tgacgtggtc gcacatcgag
      301 gagttcgccg atcagaaccg cgacggcaag gccacgcggg aggagtggtt ccagaccctc
      361 gaggacacga tccgcgacga ggcgcagtac gcgaagtacg tgacgccgtt cgcggagggc
      421 gtgttcgacc tgctggacga ggacggggac ggcgtcgtcg gtgaggacga gtacgtacgg
      481 ttcatgcgca tcttcggcgg caccgaggag aacggccgcg aggcgttccg ccacgtcgac
      541 gcggacggag acggccgcct gacccgcgac gagctgctcg agcgcgtgcg cgaatgggag
      601 accggtgagg cccccgccgc gccggggaac tggctcttcg gcgagttctg accgctaccg
      661 atcccgcggt cctggaaggg cttccgcacc ttggcctcgg cgcccgctcc gtacagccgg
      721 ctcgcgccgc cacttagcgg gacgggcggt actcgccgaa gacccggcgc agggcgtcgg
      781 agacctcgcc gagcgtcgct cgggcggcga gcgcctctcg catcggcagg agcaggttcc
      841 cggtcccgcc ggccgtctcc tcgaccgacg ccagcgccgc gtcgaccgcg cgcccgtccc
      901 gctccgcgcg cagccgtcgg acgcgctcga cctgggcggt gatggccgcc tcgtccaggt
      961 gcagcaggtc cggcggcacg tcggcctcct ccgcgtagcg gttcacgccg accacgaccc
     1021 gctcgccgct ctcgacgccc atctggatgc ggtaagcggc ctcggcgatc tcgtcctgga
     1081 tccagccggc ctcgatcgcc gccaccgcgc cgcccatccc gtcgatcttt tccaggtact
     1141 cctcggcccg cgcctcgatc tcgtcggtga gggactcgac gaagtaggcg ccggccagcg
     1201 ggtcgacggt gtcggtcacg cccgattcga acgcgatcac ctgctgggtg cgcagcgcga
     1261 ccgtcaccgc gtgctccgtc ggcagcgcca gcgcctcgtc gaacgagttc gtgtgcagcg
     1321 actgggtccc gccgagcacg gcggagagcg cctcgagcgc ggtgcggacg atgttgttct
     1381 ccggctgctg cgcggtgagc gtcgcgccgc cggtctgggt gtggaagcgc agcatggcgg
     1441 agcgcgggtc ccgcgccccg aagcgcgctc gcgcgatccg gctccacagc cgccgggcgg
     1501 cccggaactt ggcggcttcc tcgaagaagt tcatgtggca ggcgaagaag aacgacagcc
     1561 gcggcccgaa gtcgtcgatg tgcaggccgg ccgcgagtgc cgcctccacg taggcgatcg
     1621 cgttcgccag cgtgaaggcc acctcctgga ccgcggtgga gccggcctcg cgcatgtggt
     1681 acccggagat cgagatcgtg ttccacctcg gcagccgctc tcgggcgtag gcgaagaggt
     1741 ccgtgatcag gcgcatcgag ggccccggcg ggtacacgta cgtgccgcgg gcggcgtact
     1801 ccttcaggat gtcgttctgc acagtcccgc cgagcgcggc gggttcgatc ccgcgctcct
     1861 gcgcgacgat ctcgtagagc aggaggagga cggcggcggt ggcgttgatc gtcatcgacg
     1921 tcgtcacacg gtcgagcggg atgccgtcga gcaggcggtg catgtcctcc accgagtcga
     1981 tggcgacgcc ggtccgcccg acctccccct ccgcccgcgg gtggtccgag tcgagcccca
     2041 tctgcgtggg caggtcgaac gccaccgaca ggcccgtctg cccccggtcg agcaggtagc
     2101 ggaaccgccg attggtctcc tcggcggtcc ccatgccggc gtactggcgc atcgtccaca
     2161 gccggccccg gtacatcgtc ggatggatcc cgcgggtgaa cg
//
LOCUS       DASPWO010000334         4301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_817712, whole genome shotgun sequence.
ACCESSION   DASPWO010000334 DASPWO010000000
VERSION     DASPWO010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817712"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VEO00_07645"
     CDS             complement(<1..296)
                     /locus_tag="VEO00_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57907.1"
                     /translation="MEPATPTIETALGEFLEDQRARLATRTFERYRDIVELLTHCLNE
                     YGHQALDRSERRRFEKAYTSDPEAFSRLFGPDKILDNYGEFLGYFMIRKVGYGG"
     gene            407..793
                     /locus_tag="VEO00_07650"
     CDS             407..793
                     /locus_tag="VEO00_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYU57908.1"
                     /translation="MKASRADGGMVSTGEVGQALGVSINTVKSWIRAGEVEAIRLPSG
                     HYRIPRSELERLVGGGSRVSGRWQEYERWRREQPAEEVDVDRVLEWIDSILRLARSQG
                     PLLEESLEEKAEGIRRLHHALASVPR"
     gene            790..1356
                     /locus_tag="VEO00_07655"
     CDS             790..1356
                     /locus_tag="VEO00_07655"
                     /inference="COORDINATES: protein motif:HMM:NF021487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HYU57909.1"
                     /translation="MSELGDALAVVKEVLERHRVPYMVIGGVAVVVWGEPRTTRDVDV
                     TVDVGTLGTGAFVDVAAETGSPRPEHPLEFAERTHVLPVRTPADVPVDFVLATRPFEI
                     DAIRRARLVTVEGEEAPFCGPEDLVVHKIVSERLRDLEDVVGILRRQRDRLDLAGLDR
                     TVAAMAQDLDEPDVLRRYESARVAAGIR"
     gene            complement(1414..1632)
                     /locus_tag="VEO00_07660"
     CDS             complement(1414..1632)
                     /locus_tag="VEO00_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57910.1"
                     /translation="MNLIAMLVNAGVVAAVGLLLVWLGKGRFDALDQRIDRVERRMDI
                     MQASLDGLRSDLTHVALAVGVRPRATNR"
     gene            1852..2091
                     /locus_tag="VEO00_07665"
     CDS             1852..2091
                     /locus_tag="VEO00_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HYU57911.1"
                     /translation="MARAVSVRLDEEALRALARLEASGLSRSQAIRKALVDAAERLAR
                     RESVAAEARALEADEADRREMLEVAALMESMRAPG"
     gene            2078..2392
                     /locus_tag="VEO00_07670"
     CDS             2078..2392
                     /locus_tag="VEO00_07670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HYU57912.1"
                     /translation="MRRGDVYEFRPPRGIGHEQRARRFGVVVQSDALLPRSVVIVAPT
                     SRSARSASFRPEVVMGDEPTKVLVEQVGAVDATRLGDLVGHVTPEELWGIDEALLTVL
                     DL"
     gene            complement(2530..2931)
                     /locus_tag="VEO00_07675"
     CDS             complement(2530..2931)
                     /locus_tag="VEO00_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57913.1"
                     /translation="MAVDERARHELFLRVEGELGPQAAETLMQLLPPVGWADVATKHD
                     LNLRFEAFEHRLLGEIRGDINQLRAEVDGDFHQLRGEIHELRTEMHSMRGDLFQAMFE
                     QTHTLFRNLVMLCASLVFTVAGLAFAAARLS"
     gene            3211..>4301
                     /locus_tag="VEO00_07680"
     CDS             3211..>4301
                     /locus_tag="VEO00_07680"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HYU57914.1"
                     /translation="MADWALEEAASKVRAMLDPKTPPAEVAAHFLDMYEDDRLAPHGF
                     SLALGADVEGKAAEVAEEALRMAPGTLTALALAADVAEVADDLEEAEERHREAFGVLA
                     LAANGDGGHDEPAWARPGGHDPAAGDDEDDGSSTRVALSTRLAGVLVRRDRVADALDL
                     LFEVGREDPGYAPAHPSLATALADASERQAMVPAEPCPCGSGRAHGECCRPREGEALA
                     RFEDRSPMYELRDALRPFLERPEIDPFVMHGISDWYQGGTPEEVGDPGESEVRLAVER
                     ILVVAGPRPDDEHYTLLDAFADDPGTPAPLARRAREWRTFVGFGAWEVANQESAPGVL
                     LEDLVTGRFVFAAVPPEQLEHLPRWSVLLG"
BASE COUNT          633 a         1448 c         1609 g          611 t
ORIGIN      
        1 tcgccgccgt atccgacctt ccggatcatg aagtagccca ggaattcgcc gtagttgtcg
       61 aggatcttgt ccgggccgaa cagccgggag aacgcctcgg ggtcgctcgt gtacgccttc
      121 tcgaaccgcc gccgctcgga gcggtcgagc gcctggtggc cgtactcgtt caggcagtgc
      181 gtcaggagct cgacgatgtc ccggtagcgt tcgaacgtgc gggtcgccag ccgggcccgc
      241 tggtcctcga ggaactcccc gagggcggtc tcgatggtcg gggtcgccgg ttccacgggc
      301 ggacatccta ccggcgggcc gtggctggcg aacccgagct gatccagcgc ggccggcgcg
      361 ggcgcctcgc tgacgattct gatggttctg acggtaagat ggaaggatga aggcgtcgag
      421 ggcggatggg gggatggtct cgaccggcga ggtgggacag gcgctcggcg tcagcatcaa
      481 cacggtcaag agctggatca gggccgggga ggtggaagcg atccggcttc cctcggggca
      541 ttaccgcatc ccgcgaagcg agctggagcg gctcgtgggc gggggatcga gggtctccgg
      601 ccgctggcag gagtacgagc gatggcgacg cgaacagccg gccgaggagg tcgacgtcga
      661 ccgggtcctc gagtggatcg actcgatcct tcggctcgcc cgcagtcagg ggccgttgct
      721 cgaggagtcg ctcgaggaga aggcggaggg aatcaggcgt ctgcaccacg cgttggcgag
      781 cgtgccgagg tgagcgagct gggtgacgca cttgcggtgg tcaaggaggt gctggagcga
      841 catcgggttc cgtacatggt gatcggcgga gtggcggtcg tggtctgggg cgagccacgg
      901 acgaccaggg acgtcgacgt gacggtggac gtcgggaccc tcggcaccgg ggcattcgtc
      961 gacgtcgccg cagaaaccgg aagtccccgg cccgagcacc cgctcgagtt cgcggagcgc
     1021 actcacgtcc tgcccgttcg gacgcccgcc gacgtgccgg tcgacttcgt gctggcaact
     1081 cggccgttcg agatcgacgc gatccggcgg gcccgtctgg tgaccgtgga gggcgaggaa
     1141 gctccgttct gcgggcccga ggacctcgtc gtccacaaga tcgtctccga acgcctccgc
     1201 gacctggaag acgtcgtggg gatcctccgg cggcagcggg accggctcga tctcgccggc
     1261 ctggaccgca cggtcgcagc gatggctcag gatctcgacg agcccgacgt cctccggcgc
     1321 tacgagtccg ccagggtggc tgcggggatc cgctgacgaa ccgacccgcc gggagacgtc
     1381 ctggagctcc ggcccggacg gcgagaggtg gcgtcagcgg ttcgtcgccc gcggcctgac
     1441 cccgacggcc aacgccacgt gcgtgaggtc cgaccgcagg ccgtcgaggg acgcctgcat
     1501 gatgtccatc cgacgttcga ctcgatcgat ccgctggtcg agggcgtcga accgcccttt
     1561 gccgagccag accaggagca ggccgaccgc agcgacgacc cctgcgttga ccagcatggc
     1621 gatcaggttc atcggtgcgt tcctctccgg cggaggaagg ccgacctcaa cgaagcgagc
     1681 ctatcagcag gcggcgaaac gtcgaggccc ggaccaaacc gtgaccagcc attcgtccct
     1741 gtcgacacgt cgctctgacc cgcgtggaag ccgatcacgt ggccccgcga acccagcgcg
     1801 cgagctggcc gatcggcctt gtatgacgct tcgtgatacc gtttgagatc catggcgaga
     1861 gcggtgtccg ttcggctcga cgaggaggcg cttcgggcgc tggcccgtct ggaggcgtcc
     1921 gggctgagcc ggtcgcaggc catccggaag gccctggtcg atgcggccga gcggctcgcg
     1981 aggcgggaat ccgtcgcggc cgaggcgagg gcactggagg cggacgaggc cgaccggcgc
     2041 gagatgctcg aggtggccgc gctgatggag agcatgcgtg cgccggggtg acgtctacga
     2101 gttccgtccc ccgaggggga tcggtcacga gcagcgggcc cgtcgcttcg gcgtcgtggt
     2161 gcagtcggac gcgctgctcc cgcggtcggt cgtgatcgtc gcgccgacgt cccggtcggc
     2221 ccgctccgcg tcctttcgtc ccgaggtcgt gatgggggac gagccgacca aggtcctcgt
     2281 cgagcaggtc ggcgcggtcg acgccacccg cctcggcgac ctggtcggcc atgtcacccc
     2341 cgaggagctg tggggcatcg acgaggcgct cctcacggta ctcgacctgt agcaccgagc
     2401 gccgtcccga ccgacaccgg agggcacgct agcttcccgt gggttcagac catccgctcc
     2461 gtggccaacc ccgacaagct cctccacctc cagccgggcg gacggctgac acgctgatcc
     2521 gcgaaggggc tacgagagcc tggccgcggc gaacgccagg ccggccacgg tgaataccag
     2581 gctcgcgcag agcatcacga ggttgcggaa cagcgtgtgg gtctgctcga acatcgcctg
     2641 gaacaggtcg ccgcgcatcg agtgcatctc cgtgcgcagc tcgtggatct ctccgcgcaa
     2701 ctggtggaag tctccgtcga cctctgcgcg caactggttg atgtctccgc ggatctctcc
     2761 gagaaggcga tgctcgaacg cctcgaaccg caggttgagg tcgtgcttcg tcgcgacgtc
     2821 cgcccagccc accggcggga gcagctgcat cagcgtctcc gccgcctggg gaccgagttc
     2881 gccctcgacc ctgaggaaca gctcgtgtct ggcccgttca tcgaccgcca ttgtcctcct
     2941 ccttctcacg cgcgccaagc ggggctggag ggggatgcga gcgaccatac gccgaggcac
     3001 cgacacggat cggccgcctt tccggatgtc cgcggggctc ggtaccatga cccgcgcccg
     3061 cccacgggcg gcgccggaga cccgagaccg aaccttcccc ggatcggagc tcgttcgtcc
     3121 atgaccacga accggcgaaa gaagcagacg gccaggacca ggcagcgcca cgaagcggtc
     3181 cagcgcgaac gatcccgcgc gaggcgcacg atggccgact gggcgctcga ggaggccgcc
     3241 tcgaaggttc gggccatgct cgatccgaag acgcctccgg ccgaggtggc cgcgcacttc
     3301 ctggacatgt acgaggacga ccggctcgcg ccccacgggt tctcgctggc tctcggggcc
     3361 gacgtggagg ggaaggccgc agaggtcgcg gaggaggccc tccggatggc gccggggacg
     3421 ctcacggcgc tggccctggc ggccgacgtc gccgaggtgg ccgacgacct ggaggaggcc
     3481 gaggagcggc accgggaggc gttcggcgtc ctggccctgg cggcgaacgg cgatggcggg
     3541 cacgacgagc cggcgtgggc ccgcccgggc ggccacgacc cggcggccgg ggacgacgag
     3601 gacgacggtt cgtccacccg cgtcgccctg agcacccgcc tggccggcgt gctggtccgg
     3661 cgggaccgcg tcgcggacgc cctcgacctg ctgttcgagg tgggccggga ggacccgggg
     3721 tacgcgcccg cgcacccgtc cctggccacg gcgctggccg acgcgtcgga gcggcaggcc
     3781 atggtcccgg cggagccctg tccctgcggg tccggccggg cgcacggcga gtgctgccgc
     3841 ccgcgcgagg gcgaagcact ggcgaggttc gaggaccgct cgccgatgta cgagctccgc
     3901 gacgcgctcc gcccgttcct ggagcgaccg gagatcgacc cgttcgtcat gcacgggatc
     3961 tccgactggt accagggcgg gacgcccgag gaggtggggg acccggggga gagcgaggtc
     4021 cggctcgccg tcgaacggat cctggtggtg gcgggcccgc gcccggacga cgagcactac
     4081 acgctgctcg acgcgttcgc ggacgacccg gggaccccgg ctccgctcgc ccgccgggcc
     4141 cgggagtggc ggacgttcgt cgggttcggg gcgtgggagg tcgcgaacca ggagtcggcg
     4201 cccggagtgc tgctggagga cctcgtgacg gggcggttcg tgttcgcggc cgtaccgccc
     4261 gagcagctcg agcacctgcc ccggtggtcg gtcctgctgg g
//
LOCUS       DASPWO010000335         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_391160, whole genome shotgun sequence.
ACCESSION   DASPWO010000335 DASPWO010000000
VERSION     DASPWO010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391160"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2649)
                     /locus_tag="VEO00_07685"
     CDS             complement(<1..>2649)
                     /locus_tag="VEO00_07685"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HYU57915.1"
                     /translation="AAPFRECPPEIVVAMGGVVPTAQQWEAISQPLESYSIVAGAGSG
                     KTSLMAARIAYLTLVRVGRLDAGHEGALPSEILGLTFTNKAAEHLSRKTRDAVAHLGL
                     PESEEPTVMTYHAFGSAILQTYGVRLEMEPGAAILTEAQKWDLVGRVLEDREFEALQV
                     RHPPYTTGQILDLADQCANLLVDSEDVKRADAEFVESLEGVDLDDDLEKALGRARQRD
                     ELADAVAEYRGLRRGLGRIDYGDQIELATRLAESYPEVGRDLRARFRVALLDEYQDTN
                     LAQARLIRALFPPPFPLTAVGDPDQNIYAWRGASLTNLLEFTEAFGVPGRQLPLYVNF
                     RSGSRILRVADRVAERIPVERRAPGKQLIPFHGRGTGFVRAFVARDQVDEADQIARMI
                     REEHRSGRAWSDFAILCRKKRLFRVIDERLRAVDIPTEVVDVGGLLKTPEVVDLVAML
                     RVIADPARNVSLARLLLGPRWRIGYRDLARLAAWSAQHNVAFREEMAEADEDVSPGDT
                     SFAMAESLEHLDEIEDLSDQARRRLAAFRHELGALRERAGGPPAELAFAIAERSGLLA
                     EIESSAGEAAAGARQNVLSFLEHLESFAPLQGEPTLQAVLDHLDRAEQAEEEFEGTQP
                     SEENTVKLMTIHKAKGLEWPVVFVPGLAEGDRWGSSIFPDVAREENPLTSASSVPFTL
                     RPEFADFPDVHAGSKAWKAELWRRAFEAERRVCYVALTRARDVLVVSSAHWYEGSEGL
                     VREPLHPGRFYREVTRVGDDQVDPEVEVLFEDVLSDEDALPENNPLVAARAARAASWP
                     PATRVEGPDALFAQGWRRAAEAAAADASLPDRLASDLTPHERTAYEGAKAAALDRLAF
                     LAERDPAREARPLPVPAGLSVSSLVT"
BASE COUNT          354 a          999 c          932 g          364 t
ORIGIN      
        1 aggtcacgag gctcgacacc gacaggccgg cggggaccgg cagcggccgc gcttccctcg
       61 ccgggtcgcg ctccgcgagg aacgccagcc ggtcgagcgc cgcggccttc gctccctcgt
      121 aggccgtccg ctcgtgcggc gtcaggtccg acgccaggcg gtcgggcagc gacgcgtcgg
      181 ccgccgccgc ctccgccgcc cgccgccacc cctgcgcgaa gagcgcgtcg ggcccctcga
      241 cccgcgtcgc cggcggccac gacgccgccc gcgcggcccg cgccgccacg agtgggttgt
      301 tctcgggaag cgcgtcctca tccgacagga cgtcctcgaa gaggacctcc acctcggggt
      361 cgacctggtc gtcccccacc cgcgtcacct cccggtagaa gcgcccggga tggagcggct
      421 cgcggacgag gccctccgac ccctcgtacc agtgagccga ggagacgacc aggacgtcgc
      481 gcgcccgcgt cagcgcgacg tagcaaacgc ggcgctccgc ctcgaacgcg cggcgccaca
      541 gctccgcctt ccacgctttc gatccggcgt ggacgtccgg gaagtcggcg aactcgggcc
      601 gcaacgtgaa cggcaccgac gaggccgagg tgagcgggtt ctcctcgcgt gcgacgtcgg
      661 ggaagatcga cgacccccag cgatcgccct cggcgaggcc gggcacgaac accaccggcc
      721 actccaggcc cttcgccttg tggatcgtca tcagcttcac cgtgttctcc tccgagggct
      781 gggttccctc gaactcctcc tcggcctgct cggcccggtc gaggtggtcg aggaccgcct
      841 gaagcgtcgg ctcgccctgg agcggcgcga acgactcgag gtgctcgagg aacgagagca
      901 cgttctggcg ggcgccggcg gccgcttcgc cggccgacga ctcgatctcc gcgagcagtc
      961 cgctgcgctc cgcgatggcg aaggccagct ccgccggcgg ccccccggcc cgctcccgca
     1021 gcgccccgag ctcgtggcgg aacgcggcca ggcgccggcg ggcctggtcg gacaggtcct
     1081 cgatctcgtc gaggtgctcg agcgactcgg ccatggcgaa cgaggtgtcg cccggcgaca
     1141 cgtcctcgtc cgcctcggcc atctcctcgc ggaaggcgac gttgtgctgc gcggaccagg
     1201 ccgcgagcct cgccaggtcg cggtagccga tccgccagcg ggggccgagc agcagccggg
     1261 ccaggctgac gttgcgggcc gggtcggcga tcacccggag catcgccacc aggtcgacca
     1321 cctccggcgt cttcagcagc ccgccgacgt ccaccacctc ggttgggatg tcgaccgcgc
     1381 ggaggcgctc gtcgatgacc cggaacagtc gcttcttgcg gcagaggatg gcgaagtcgc
     1441 tccacgcccg cccgctcctg tgctcctcgc ggatcatccg ggcgatctgg tcggcctcat
     1501 cgacctggtc ccgcgcgacg aacgcgcgca cgaatccggt tccccgcccg tggaacggga
     1561 tgagctgctt gcccggcgcg cggcgctcga ccgggatgcg ctcggccacc cggtcggcca
     1621 cacgcaggat ccggctcccc gatcggaagt tgacgtacag gggcagctgg cggccgggca
     1681 ctccgaaggc ctcggtgaac tccagcaggt tcgtcaggct ggccccgcgc caggcgtaga
     1741 tgttctggtc ggggtcgccc accgcggtca gcggaaacgg cggcgggaac aacgcccgga
     1801 tcaggcgtgc ctgcgccagg ttcgtgtcct gatactcgtc gagcagcgcg acccggaagc
     1861 gcgcgcgcag gtcgcggccg acctccgggt acgactcggc caggcgggtg gcgagctcga
     1921 tctggtcccc gtagtcgatc cggccgagtc cccggcgaag cccccggtac tcggcaacgg
     1981 cgtccgccag ctcgtcgcgc tgccgggcgc ggcccagcgc cttctcgagg tcgtcgtcga
     2041 ggtccacgcc ctcgaggctc tcgacgaact cggcgtcggc gcgcttcacg tcctcggaat
     2101 cgacgagcag gttcgcacac tggtccgcga ggtcgaggat ctggccggtc gtgtacggcg
     2161 ggtggcggac ctggagcgcc tcgaactcgc ggtcctccag cacccgcccc accaggtccc
     2221 atttctgtgc ctcagtcagg atcgccgcgc ccggctccat ctcgagtcgc acgccgtacg
     2281 tctggaggat ggcggacccg aacgcgtggt aggtcatcac ggtcggctcc tccgattccg
     2341 gcagcccgag gtgcgccacc gcgtcgcgcg tcttgcgcga gaggtgctcg gcggccttgt
     2401 tcgtgaacgt gagtccgagg atctcggagg gcagggcccc ctcgtggccg gcgtccagcc
     2461 ggccgacgcg gacgagcgtc aggtaggcga tccgggcggc catcagcgag gtcttgcccg
     2521 acccggcccc ggcgacgatc gagtaggact cgaggggctg gctgatcgcc tcccactgct
     2581 gggccgtcgg caccacgccc cccatcgcga cgacgatctc cggcgggcat tcgcggaagg
     2641 gggccgccg
//
LOCUS       DASPWO010000336        10007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1070927, whole genome shotgun sequence.
ACCESSION   DASPWO010000336 DASPWO010000000
VERSION     DASPWO010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10007
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070927"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VEO00_07690"
     CDS             complement(<1..764)
                     /locus_tag="VEO00_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HYU57916.1"
                     /translation="MRSVWLIPALPLAGAALLLVSGKRLRRVAGGLAAGLVGASFVIG
                     VLVFIDLSGLAGDSRTFVQTLWEWVPVGAFRVPVELRVDTLSMVMALTVTGVGFLIHV
                     YSIDYMHGDPRYTRFFAYMNLFVFFMLTLVLANNFLLLYVGWEGVGLCSYLLIAFWFE
                     KRAAADAGKKAFIVTRVGDTGMLIGIILLFTTVGSVDFDRVFAEAPALANGTATAISL
                     LLFAGAVGKSAQLPLSTWLPDAMEGPTPVSALIHAAT"
     gene            complement(767..1072)
                     /gene="nuoK"
                     /locus_tag="VEO00_07695"
     CDS             complement(767..1072)
                     /gene="nuoK"
                     /locus_tag="VEO00_07695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HYU57917.1"
                     /translation="MKTPIAYYLILSAILFAIGTIGVLVRRNALVIFMSVELQLNAIN
                     LTFVAFSRQLGTLDGQVLAFFSMVVAAAEVVVGLAVIVAVFRRQHSVNVDDASLLKG"
     gene            complement(1069..1644)
                     /locus_tag="VEO00_07700"
     CDS             complement(1069..1644)
                     /locus_tag="VEO00_07700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HYU57918.1"
                     /translation="MDLVIFWVLAPVSVAAAVAMLFQRDAVHSALLLIVNFFTLAVFF
                     LLLDAPFLFVVQIIVYAGAIMVLFLFVIMLLGVQPEESLVERLAGQRWVAILLGAGVV
                     LEVFAAVRLAVGLGRGAVPDFAAANAGGNVRALARVLFTGYVFPFEVTSLLLIIAAIA
                     AIVLARRRERAARAAEPEAPPVEVAEREPVS"
     gene            complement(1689..2105)
                     /gene="nuoI"
                     /locus_tag="VEO00_07705"
                     /pseudo
     CDS             complement(1689..2105)
                     /gene="nuoI"
                     /locus_tag="VEO00_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225886.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
     gene            complement(2102..3286)
                     /gene="nuoH"
                     /locus_tag="VEO00_07710"
     CDS             complement(2102..3286)
                     /gene="nuoH"
                     /locus_tag="VEO00_07710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HYU57919.1"
                     /translation="MDWLDWLLLGARVVAVFFALLVGVMLVIWIERKVIADMQTRIGP
                     NRAGPFGLLVTLADGVKLFFKEGVTPAAADRPVYVLAPIIQLIPAFLAFAVIPFGTSV
                     TVFGRTIPFQIADLNVGILWVLAMGSLAVYGIVLAGWSSGSNYPLLGSIRSSAQLISY
                     EVGMGLGIVAVLMYTGSLLMSDIVAFQARHFEATGFGWLPKWNVFAQFPAFAIYATAA
                     LAETNRPPFDLPEAETELVAGYSTEYSGIKFAMFYLSEYLHTITISAIGVTLFFGGWR
                     GPQFSFLSWLWPLVWFLLKVFLFVFVFVWIRATLPRFRYDRLMNLGWKVLIPIGLFWV
                     LATGAIVVLPDVYGPGPFRTALLVVAAILLLLYVAPLFNPRARQEQAAPADGQAKKGG
                     ST"
     gene            complement(3286..5712)
                     /locus_tag="VEO00_07715"
     CDS             complement(3286..5712)
                     /locus_tag="VEO00_07715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HYU57920.1"
                     /translation="MSDPVVTVTIDGKEITVSANSLIIRAAEALGIEIPRFCDHPLLE
                     PVAACRQCYVSVEGQRKLVTSCSTPVADGMVVHTQNTSAEVKDAQEAVLEFLLINHPL
                     DCPICDRGGECPLQDQALGFGKGVSRYVEPKRTYRKPIPLSPLVNLDRERCVLCARCT
                     RFCDQISGDRFIELFSRGAAEQVSIAPGEDFDSPFSGNTVQICPVGALTSSTYRFAAR
                     PFDIADGDSICPHCACGCSIRVDTRRGEVVRHLARDNPDVNEAWLCDKGRYSFRASDR
                     DRLELPLLRRRGGLEPAPYEEVFSAVADWSASGRVAFVGGGRLSDEDDYALAKLARTA
                     FRTNDLDHRLLGSPDVPVDVDRLAAAGMPVTYADVERARVILLVGLDAEQELPILHLR
                     LRKADRAHGARIFVIHPRRTRLHDVSEHILVPPGSEAAALERLMRSEGLETAFGRAHY
                     VLREAGADGVILAGPRLAESAGAVAAAFALARETGARFAYLCRRANDRGALVAGVHPA
                     LLPGGRSVEDDRERAEIEVAWETLVPRRPGRDTAGILQAAANREVDVLFLLGADPLRD
                     FPDHGLALRGLQNVRYKVVADTVRDGLSIFADAMLPAAAFLEKDGHYTDWEGRSQRLR
                     PVRAPVGLARSDWEILQGLSEVLGRSLGFGSLDDLRAEMGRFVAPRDLGAVRRPTLAA
                     GTAFGEPDGELTLFTYPLLVDEGRLSEGADELKAALEQPPALEVHPEDARALGLVPGE
                     PAKVVTAAGEARLAVRVSDGIARGCAFVPYNQPGFAASALLSGRHHVQARVEGPAGVA
                     AAPAREESEA"
     gene            complement(5709..7049)
                     /gene="nuoF"
                     /locus_tag="VEO00_07720"
     CDS             complement(5709..7049)
                     /gene="nuoF"
                     /locus_tag="VEO00_07720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HYU57921.1"
                     /translation="MRVVTARFGDPGAAPLDGYRASGGYEGLRKALAMSPEEVIEEVK
                     ASGLRGRGGAGFPTGTKWSFVPQDTGKFTYVVCNFDESEPGTFNNRELVERDPHQVIE
                     GLAIAAYAVRCHTAFVYCRGEFVWPGLVLQRALAEAYDAGILGNRVFGSDFQLDVVLH
                     RGAGAYICGEETALLESLEGYRGQPRLRPPFPAVEGLYASPTVINNVETLSNVPHILV
                     NGADWYAGIGTQKSTGTKVFSVSGKVERPGNYEVPMGTPAGEVLALAGGVLGGKALKA
                     WTPGGSSTPFLTADHLGVGLDFESIAEAGSLLGTGALIVLDETDCIVEAAMRLTEFYA
                     HESCGKCTPCREGTWWLTKVYRRLEEGLGRKEDLPLLADVSDNMLFKSFCALADGAVS
                     PISSSLKHFYAEYEAHVTGQGCPLKRDHALVGAVTARTGSTEGGGSIAEGEVLP"
     gene            complement(7054..7635)
                     /gene="nuoE"
                     /locus_tag="VEO00_07725"
     CDS             complement(7054..7635)
                     /gene="nuoE"
                     /locus_tag="VEO00_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HYU57922.1"
                     /translation="MTIPKDFAAQAREIVERYPHLRSALMPLLYLAQSLEGHVSREGM
                     QAVASALGLTTAEIEAVVTFYTMYRRPGCGRYLVSICTNLSCALRGGRRLMELVLEEL
                     GPEALEATADGAITVHEEECLAACDAAPVVQVNWLNYDRVSEDDLRHLLAALRDGTPP
                     PPARGPVPGTLSAVSRVLAGLEEAVDQGDSTGG"
     gene            complement(7712..9001)
                     /gene="nuoD"
                     /locus_tag="VEO00_07730"
     CDS             complement(7712..9001)
                     /gene="nuoD"
                     /locus_tag="VEO00_07730"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HYU57923.1"
                     /translation="MSGTDGRVESSGNGYGGEPGTSGGRREDTMIVNMGPQHPSTHGV
                     LRLVLELDGETVISCRPVVGYLHTGIEKNTEVRTWQQGVTFLTRADYLSPFFNELAYC
                     LAVERLLGVEAPPRAQVIRVLVCELNRIASHLVWLATTGLELGAVSVMLYGFRERERI
                     LDIFEAFTGLRMNHSYIRIGGLAMDVPPGGLEMIRSFLDEMPKRIDDYEALLSRNPIW
                     LERTRGVGHVSAERLLALGVTGPILRAAGVAYDVRVDQPYSGYERYEFDVPTETESDC
                     YARYRVRVAEMRESLKIVRQAVDRLPGGKVMIDDPHLAWPAQLSVGPDGIGNSEEYVR
                     YIMEESMEALINHFKIVTQGIRVPPGEVYQTVESPRGELGFYVVSSGTTQPYRVAVRD
                     PSFVNLQSLPPMVEGGLVADVIASVASVDPVMGGVDR"
     gene            complement(9004..9504)
                     /locus_tag="VEO00_07735"
     CDS             complement(9004..9504)
                     /locus_tag="VEO00_07735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYU57924.1"
                     /translation="MEAEALAIRVRDRFPDTQVARGEVTVTVAPADLQPALRFLRDAE
                     ALSFDFLSGIACTDWPDGEPRFWLAYHLWSMAHRHRVRVKVGLAGDEPRVPSVVSMFP
                     TANWEEREVYDFFGVRFDGHPDLTRIIMPDDWEGHPLRKDYGLGGVTTYYKGGAEIPP
                     PDRRTY"
     gene            complement(9529..9948)
                     /locus_tag="VEO00_07740"
                     /pseudo
     CDS             complement(9529..9948)
                     /locus_tag="VEO00_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
BASE COUNT         1618 a         3643 c         3442 g         1304 t
ORIGIN      
        1 gtcgcggcgt ggatcagcgc cgacaccggc gtggggccct ccatcgcgtc ggggagccac
       61 gtggacagcg gcaactgggc cgacttgccc accgcgccgg cgaacagcag caggctgatc
      121 gcggtggccg tcccgttcgc cagcgcgggg gcctcggcga agacccggtc gaagtcgacc
      181 gagccgaccg tcgtgaagag caggatgatc ccgatcagca tccccgtgtc gccgacccgg
      241 gtcacgatga aggccttctt cccggcgtcg gcggccgcgc gcttctcgaa ccagaacgcg
      301 atcagcaggt acgagcagag cccgacgccc tcccacccga cgtacagcag caggaagttg
      361 ttcgccagga caagggtgag catgaagaag acgaacaggt tcatgtaggc gaagaaccgc
      421 gtgtagcgcg ggtcgccgtg catgtagtcg atcgagtaga cgtggatcag gaaccccacc
      481 ccggtgacgg tcagtgccat gaccatcgac agcgtgtcga cccgcagctc caccggcacg
      541 cggaacgcgc cgaccgggac ccactcccac agggtctgga cgaaggtccg gctgtccccc
      601 gccagccccg agaggtcgat gaacaccagc acgccgatca cgaacgacgc cccgaccagc
      661 cccgcggcca gcccgccggc cacgcgccgc agccgcttgc cggacaccag cagcagggca
      721 gcacccgcca ggggcagcgc gggaatgagc cagacgctcc gcaccgctac cccttcagca
      781 ggctggcgtc gtcgacgttg accgagtgct ggcgccggaa gaccgcgacg atgacggcga
      841 gcccgacgac gacctcggcc gcggcgacga ccatcgagaa gaaggccagc acctggccgt
      901 cgagcgtccc gagctggcgg gagaacgcca cgaaggtcag gttgatcgcg ttcagctgca
      961 gctcgaccga catgaagatc accagggcgt tgcgccggac gagcacgccg atcgtcccga
     1021 tcgcgaacag gatcgccgac aggatcaggt agtaggcgat cggcgtcttc acgacaccgg
     1081 ctcccgctcc gcgacctcca ccgggggcgc ctccggctcc gccgcccgcg cggcccgctc
     1141 gcgccgccgg gccaggacga tcgccgcgat cgcggcgatg atcagcagca gcgaggtgac
     1201 ctcgaagggg aatacgtacc cggtgaacag gacccgggcg agcgctcgca cgttcccgcc
     1261 agcgttcgct gccgcgaagt ccgggacggc accccggccc aggccgaccg ccagacgcac
     1321 cgcggcgaac acctcgagca cgacgccggc gccgagcagg atcgccaccc agcgctgccc
     1381 ggccagccgc tcgacgaggg actcctcggg ctgcacaccg agcagcatga tcacgaacag
     1441 gaacagcacc atgatcgcgc cggcgtagac gatgatctgc acgacgaaca ggaacggcgc
     1501 gtcgagcagc aggaagaaca ccgcgagcgt gaagaagttg acgatcaaga gcagcgccga
     1561 gtggacggcg tcgcgctgga agagcatcgc caccgcggcg gccaccgaga ccggcgccag
     1621 gacccagaag atgacgaggt ccactaccga ccctccttgg tccccggccc ggtcccgagc
     1681 gggggcggcg gctgcagcag ctcctccttc tccatgacgg cctcctcgcg ggtcaggaac
     1741 gccagctcgt agttcgaggt gagcgtcagc gcgcgcgtcg ggcaggcctc gacgcacagg
     1801 ccgcacatga tgcagcgcag gtagttgatc tggtagacct cggcgtaccg ttcccccacc
     1861 gagatcggct tggccgggtc gttgtccgcg ccctgcacgt agatcgcgtc cgccgggcag
     1921 gcccaggcgc acaattcgca cccgatgcac ttctcccggc cgtccgggtg gcggttcagc
     1981 aggtgccggc cgacgtggga gcgctccggc aacggccgga tctccttcgg gtactgggtg
     2041 gtcacgtcgc gccggaagat ctgcttgaac gtggtccaca gccccttgcc cagcgccccg
     2101 atcacgtgga gccacccttc ttggcctgac cgtccgccgg tgcggcctgc tcctgccggg
     2161 cgcgcggatt gaagaggggt gcgacgtaga gcaggagcag gatcgcggcg accacgagga
     2221 gcgcggtgcg gaacgggccc gggccgtaca cgtcggggag cacgacgatc gccccggtgg
     2281 ccaggaccca gaacaggccg atcgggatca gcaccttcca gcccaggttc atcagccggt
     2341 cgtagcggaa ccgcgggagc gtggcccgga tccagacgaa gacgaagacg aacaggaaga
     2401 ccttcagcag gaaccagacc agcggccaga gccaggacag gaacgagaac tgcggccccc
     2461 gccagccgcc gaagaagagc gtcacgccga tcgccgagat ggtgatcgtg tgcaggtact
     2521 ccgacaggta gaacatcgcg aacttgatgc cggagtactc ggtgctgtag ccggccacga
     2581 gctcggtctc cgcttcgggc aggtcgaacg gcgggcggtt cgtctcggcg agcgccgccg
     2641 tggcgtagat cgcgaacgcc gggaactgcg cgaagacgtt ccatttcggc agccacccga
     2701 acccggtggc ctcgaagtgg cgggcctgaa acgccacgat gtcggacatc agcaggctgc
     2761 ccgtgtacat cagcacggcg acgatcccca gtcccatccc gacctcgtag gagatgagct
     2821 gggcggacga gcggatcgat ccgagcagcg ggtagttgct ccccgaggac cagccggcga
     2881 gcacgatccc gtagacggcg agcgatccca tggccagcac ccagaggatt ccgacgttca
     2941 ggtcggcgat ctggaacggg atcgtccgcc cgaagacggt cacgctcgtg ccgaacggga
     3001 tcacggcgaa cgccaggaac gccgggatga gctggatgat cggcgccaga acgtagacgg
     3061 ggcggtcggc agcggcgggc gtgacaccct ccttgaagaa gagcttcacc ccgtcggcca
     3121 gggtgaccag cagcccgaac gggccggcgc ggttcgggcc gatccgggtc tgcatgtcgg
     3181 cgatcacctt gcgctcgatc cagatcacga gcatcacgcc gacgagcagc gcgaagaaga
     3241 ccgccaccac ccgggcgccg agcaggagcc agtccagcca gtccatcagg cctccgactc
     3301 ctcgcgcgcc ggcgcggccg ccacgccggc cggcccctcc acccgggcct gaacgtggtg
     3361 gcggcccgag agcagcgcgc tcgcggcgaa cccgggctgg ttgtacggca cgaacgcgca
     3421 cccgcgggcg atgccgtcgc tgacgcgcac cgcgaggcgg gcctccccgg ccgcggtgac
     3481 caccttcgcc ggctcgccgg ggacgagacc gagcgcccgg gcatcctccg gatggacctc
     3541 gagcgccggc ggctgctcga gcgccgcctt cagctcgtcc gccccctcgg acagccgccc
     3601 ctcgtccacc agcagcgggt acgtgaagag cgtcagctcg ccgtcgggct cgccgaaggc
     3661 ggtcccggcg gcgagcgtcg gccggcgcac cgcgccgagg tcccgcgggg cgacgaatcg
     3721 ccccatctcc gcgcgcaggt cgtcgagcga cccgaacccc aggctccgcc cgagcacctc
     3781 cgaaagcccc tgcaggatct cccagtcgct gcgggccagg ccgaccggcg cgcggaccgg
     3841 ccgcaaccgc tgggagcggc cttcccagtc ggtgtagtgc ccgtccttct ccaggaacgc
     3901 ggccgccggg agcatcgcgt cggcgaagat cgagaggccg tcgcgcacgg tgtcggccac
     3961 caccttgtag cggacgttct gcagcccgcg cagggccagt ccgtggtcgg gaaagtcgcg
     4021 cagcggatcg gcgccgagca ggaacagcac gtcgacctcg cggttcgccg ccgcctgcag
     4081 gatcccggcg gtatcgcggc ccggccgccg cgggacgagt gtctcccagg ccacctcgat
     4141 ctccgcgcgc tcgcggtcgt cctcgaccga gcggccgccg gggagcagtg ccgggtggac
     4201 gccggccacg agcgccccgc gatcgttggc gcgccggcac aggtacgcga accgggcccc
     4261 ggtctcccgg gccagggcga acgcggcggc gacggcgccg gccgactcgg ccaggcgcgg
     4321 gccggccagg atcaccccgt cggcgcccgc ctcgcgcagc acgtagtggg cccgcccgaa
     4381 cgcggtctcc aggccctcgc tgcgcatcag ccgctcgagc gcggcggcct cgctccccgg
     4441 cggcaccagg atgtgctccg agacgtcgtg cagccgcgtc cgccgcgggt ggatgacgaa
     4501 gatgcgggcc ccgtgcgccc gatcggcctt gcgcaggcgc aggtgcagga tcggcagctc
     4561 ctgctcggcg tcgagcccga cgagcaggat cacccgggcc cgctcgacgt ccgcataggt
     4621 caccggcatg ccggcggcgg ccagtcggtc gacgtccacg gggacgtccg gcgagccgag
     4681 cagccggtgg tcgaggtcgt tcgtccggaa cgccgtgcga gccagcttgg ccagcgcgta
     4741 gtcgtcctcg tccgagagcc ggccgccgcc gacgaacgcc acgcggccac tcgcgctcca
     4801 gtcggccacc gcgctgaaga cctcctcgta cggggccggc tccaggccac cccgccgccg
     4861 caagagcggc agctccaggc gatcccggtc ggaggcccgg aacgagtacc ggcccttgtc
     4921 gcacagccag gcctcgttga cgtcggggtt gtcgcgcgcc aggtgccgca ccacctcgcc
     4981 gcggcgcgtg tccacgcgga tcgagcagcc gcaggcgcag tgcgggcaga tcgagtcgcc
     5041 gtcggcgatg tcgaacggcc gcgcggcgaa ccggtacgtg ctggaggtca gcgcgccgac
     5101 cgggcagatc tggacggtgt tgcccgagaa cgggctgtcg aagtcctcgc ccggggcgat
     5161 cgacacctgc tccgcggcgc cacggctgaa cagctcgatg aaccggtcgc ccgagatctg
     5221 gtcgcagaag cgggtgcagc gcgcacagag gacgcagcgc tcgcggtcga ggttgacgag
     5281 cggcgacagc gggatcggct tgcggtaggt gcgcttcggc tccacgtagc gagagacgcc
     5341 cttcccgaag cccagggcct ggtcctggag cgggcactct ccgccgcggt cgcagatcgg
     5401 gcagtcgagg gggtggttga tcaacaggaa ctcgaggacg gcctcctgcg cgtccttcac
     5461 ctccgcgctg gtgttctggg tgtgcaccac catcccgtcg gccaccgggg tcgaacagga
     5521 cgtcacgagc ttgcgctgcc cctcgaccga cacgtagcac tgccggcagg cggccaccgg
     5581 ctcgagcagc gggtggtcgc agaaccgcgg gatctcgatg ccgagcgcct cggcggcgcg
     5641 gatgatcagg gagttcgccg acacggtgat ctccttgccg tcgatcgtca ccgtcacgac
     5701 cggatcgctc acggaagcac ctcgccctcg gcgatcgagc cgccgccctc ggtcgagccg
     5761 gtccgcgccg tcacagcgcc gacgagcgcg tggtcgcgct tcagggggca cccctgcccg
     5821 gtgacgtgcg cctcgtactc ggcgtagaag tgcttcagcg aggagctgat cggtgagacg
     5881 gcgccgtcgg ccagcgcgca gaacgacttg aagagcatgt tgtcggacac gtccgccaag
     5941 aggggcaggt cctccttgcg ccccaatcct tcctccagcc ggcggtagac cttcgtcagc
     6001 caccacgtcc cttcccggca cggcgtgcac ttgccgcatg attcgtgggc gtagaactcg
     6061 gtcaggcgca tggccgcctc gacgatgcag tcggtctcgt cgagcacgat cagcgcgccg
     6121 gtcccgagca gggagcccgc ctcggcgatc gactcgaagt cgagcccgac gccgaggtgg
     6181 tcggcggtca ggaacggggt cgaggagccg cccggcgtcc aggccttcag cgccttcccg
     6241 ccgagcaccc cgccggcgag cgccagcacc tcgcccgccg gcgtgcccat cgggacctcg
     6301 tagttccccg gccgctccac cttccccgag accgagaaga ccttcgtccc ggtggacttc
     6361 tgcgtgccga tccccgcgta ccagtcggcc ccgttcacca ggatgtgcgg aacgttgctg
     6421 agcgtctcga cgttgttgat cacggtcggg ctggcgtaca ggccctcgac cgccgggaac
     6481 ggagggcgca ggcgtggctg cccgcgatac ccctcgagcg actcgagcag cgccgtctcc
     6541 tcaccgcaga tgtaggcgcc cgccccccgg tgcagcacga cgtcgagctg gaagtcggag
     6601 ccgaacacgc ggttgccgag gatcccggcg tcgtaggcct cggccagcgc gcgctgcagg
     6661 acgagccccg gccagacgaa ctcaccgcgg cagtagacga acgcggtgtg gcagcgcacc
     6721 gcgtacgcgg cgatggcgag gccctcgatg acctgatggg gatctcgctc gacgagctcg
     6781 cggttgttga acgttcccgg ctcggactcg tcgaagttgc agacgacata ggtgaacttc
     6841 ccggtgtcct gcggcacgaa cgaccacttc gtcccggtcg ggaagccggc tccgccgcgc
     6901 ccgcgcagtc ccgacgcctt gacctcctcg atgacctcct ccggagacat cgccagcgcc
     6961 ttgcgcagac cctcgtagcc gccgctggcg cggtagccgt cgagcggcgc ggcgcccgga
     7021 tcgccgaacc gggccgtgac gacccgcatc tcctcagcca ccggtcgagt ccccctggtc
     7081 gacggcctcc tcgaggccgg ccagcacgcg cgacaccgcg gagagcgtcc cggggaccgg
     7141 gccgcgcgcc ggcggtggcg gggtcccgtc gcgcagcgcc gcgagcaggt ggcggaggtc
     7201 gtcctcggac acccggtcgt agttcagcca gttcacctgc acgaccgggg ccgcgtcgca
     7261 ggcagcgagg cactcctcct cgtggaccgt gatcgcgccg tccgccgtgg cctccagcgc
     7321 ctccggcccg agctcctcga gcaccagctc catcagtcgc cgcccgccgc gcagcgcgca
     7381 cgacaggttc gtgcagatcg acaccaggta gcggccacag cccggccgcc ggtacatcgt
     7441 gtagaaggtg acaacggcct cgatctcggc cgtcgtcagg ccgagcgccg aggccacggc
     7501 ctgcatcccc tcgcgcgaga cgtgcccctc gagcgattga gccaggtaga ggagcggcat
     7561 cagcgccgac cgcaggtggg gatagcgctc gacgatctcc cgcgcctgcg ccgcgaagtc
     7621 cttcggaatc gtcattgggc ggttcgctcg ccggacatgg gcgggcgggt cgctccacag
     7681 ggcgagctac ccccccaaac cccccgcggg atcaccggtc cacgcctccc atgacggggt
     7741 cgacgctcgc gacggaggcg atgacgtcgg cgaccaggcc gccctcgacc atcggaggaa
     7801 gcgactgcag gttgacgaag gacgggtcgc gcaccgccac ccggtagggc tgggttgtgc
     7861 cgctcgacac gacgtagaag ccgagctcgc cgcggggcga ctccaccgtc tggtagacct
     7921 cgccgggagg gacgcggatg ccctgggtga cgatcttgaa gtggttgatc agcgcctcca
     7981 tcgactcctc catgatgtag cgcacgtact cctcggagtt gccgatgccg tccgggccca
     8041 cgctgagctg cgccggccag gccaggtgcg gatcatcgat catcaccttg ccgccgggaa
     8101 gccggtcgac cgcctgccgc acgatcttca gcgactcgcg catctcggcc acccggaccc
     8161 ggtatcgcgc gtagcagtcg ctctcggtct ccgtcgggac gtcgaactcg tagcgctcgt
     8221 agcccgagta gggctggtcc acacggacgt cgtaggccac gccggccgcg cgcaggatcg
     8281 gaccggtcac gccgagcgcc aggagccgct ccgcggagac gtggcccacc ccgcgggtcc
     8341 gctccagcca gatcgggttc cgcgacagca gcgcctcgta gtcgtcgatc cgcttcggca
     8401 tctcgtcgag gaacgaccgg atcatctcca ggccgcccgg cgggacgtcc atcgccagcc
     8461 cgccgatccg gatgtaggag tggttcattc gcaggcccgt gaaggcctcg aagatgtcga
     8521 ggatccgctc ccgctcacgg aagccgtaca gcatcaccga caccgcgccg agctcgagcc
     8581 cggtggtggc gagccagacc aggtggctgg cgatccggtt cagctcgcac accaggaccc
     8641 ggatgacctg agcgcgcggc ggggcctcga ccccgagcag gcgctcgacg gccaggcagt
     8701 aggccagctc gttgaagaag ggcgacaggt aatcggcccg cgtcaggaac gtcacgccct
     8761 gctgccacgt ccggacctcg gtgttcttct cgatgccggt gtgcaggtag ccgaccacag
     8821 gacggcagga gatcacggtc tcgccgtcga gctcgaggac caggcggagc accccgtgcg
     8881 tcgaggggtg ctgcggaccc atgttcacga tcatggtgtc ctcgcgccgc ccaccggacg
     8941 tccccggctc gccgccgtag ccgttcccgg aagactcgac gcggccgtcc gtcccgctca
     9001 tgctcagtag gtccgccggt cgggcggagg gatctccgcg ccgcccttgt agtaggtggt
     9061 caccccgccg aggccgtagt ccttgcgcag cggatggccc tcccagtcgt ccggcatgat
     9121 gatccgggtc aggtcggggt ggccgtcgaa ccgcacgccg aagaagtcgt agacctcccg
     9181 ctcctcccag ttcgcggtcg ggaacatcga caccaccgac ggcacgcgcg gctcgtcgcc
     9241 cgcgagcccg accttcaccc gcacccggtg cctgtgcgcc atggaccaca ggtggtaggc
     9301 gagccagaac cgcggctccc cgtcgggcca gtccgtgcag gcgatccccg agaggaagtc
     9361 gaacgacagc gcctcggcgt cgcgcaggaa ccgcagcgcc ggttgcagat cggcgggcgc
     9421 gacggtgacg gtgacctcac cccgcgcgac ctgggtgtcc gggaaccggt cgcgcacgcg
     9481 gatcgccagc gcctctgcct ccacctagcg gaaccgccct tcggtctgga tccggtcgtg
     9541 cagcgtcagg atcccgtaca tcagcatctc cggccgcggc gggcacccgg gcacgtagat
     9601 gtcgaccggc acgatctggt ccacgccctg aaccagcgcg tagttgttga acatgccgcc
     9661 ctgcgaggcg cagacgccca tcgagatgac ccacttcggc tcgggcatct ggtcgtagat
     9721 cgtgcgcagg accggcgcca tcttctgcga caggcgcccg gcgacgatca tcaggtcggc
     9781 ctgccggggc gaggcgcgga agatctccat cccccagcgg gccaggtcgt agttcgccgc
     9841 gccggtcgcc atcatctcga tcgcgcagca cgccaggccg aacgtcaccg gccagagcga
     9901 ctgctttcgc gcccacgaca cggcccggtc gatcgtcgtc agcaggatgc cgcgctcggc
     9961 tttctcttcg ggagcggccg gcgacccccc gcgctcccac tcgaccg
//
LOCUS       DASPWO010000337         7036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_561864, whole genome shotgun sequence.
ACCESSION   DASPWO010000337 DASPWO010000000
VERSION     DASPWO010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561864"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VEO00_07745"
     CDS             <1..689
                     /locus_tag="VEO00_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU57925.1"
                     /translation="WGFDRRLAMGKGLNVLFAGPSGTGKTMAAEVLAHEIGLDLYKID
                     LATVISKWIGETEKNLAAAFEAAERGGMALLFDEADALFGKRTEVGDARDRYANLETA
                     YLLSRLERFDGLAMLASNLRQNLDSAFARRLEFIVPFDLPDTEQRVALWTRHIPPGAP
                     LASGVRLERLAALYPLPGALIRNAAVAAAFLAAAEGSDIEPRHLVHAIEREYVKAGDA
                     FPGPPPEDAQ"
     gene            730..2097
                     /locus_tag="VEO00_07750"
     CDS             730..2097
                     /locus_tag="VEO00_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57926.1"
                     /translation="MTNPVKQFRIDEKARQGMAVQAAQTELGQIDGDLATSRDTVAVN
                     TRDLAAQQAEESRLRQAIAAAAMPADADALVDQLEDNLKQQSVSSAALNDAIDQAATL
                     ERSRLRVAAELDAARATQAQAGADLAIASADDEVAERWRTAATGTAVGNAVAAAGSAS
                     TTALRTAARTRLQSLAGGSTLLDLFKRRFEEARADSDERRAKVVRAQGALDTVATKQD
                     PVNGPVVVAGAGYAAAREVVRAIAETTVQRLDAALLALQTASALGNLTTDEQTRIDDR
                     ATHATAVTGTTSSVSAASDVLDARKDVRVAEVAFDVAALAKEATDSTFDRDTDASVKT
                     KRDAWNAAKAALSAAEGKVTPTRQQQLDDWEVAIPVGVSSAVVGFLRASAEVDAIKGL
                     DPQGRVNDLNAAETTYAGALQDRTDAAELRMARALEVAVRLEAEAAVEPVADVREAAK
                     VRGDR"
     gene            2108..3433
                     /locus_tag="VEO00_07755"
     CDS             2108..3433
                     /locus_tag="VEO00_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57927.1"
                     /translation="MTTSLGDRPPAASLAAEPYTSLAVHYGMLLGVPDFELMMSNPRG
                     KLRVHNAWLHGAGVVWGYPVSVDAPSCELRVGPGLAVDGLGRELPLTMLACLDVGAWF
                     DQLDDADRVALGTSEIDGGLQFHAHLVARAAPCLARPVPAMGSNCEGGASETAYSRVQ
                     ETVELLLRAGPAPDPPPAAFPSLRRLLAAGDTGVPGDRERIEALATADREREWLDAFH
                     RLAADEVAALLPPGLVEGGAGSSTMFPVDEPGEVLLADLPDLQLVTQADGARTVAAPT
                     IDLSVRSSHVPTLVLADLLAELLTRGGGAADDADAGGPRVASVSRAGITVTVELTADV
                     VDGTLADALSLHSIDPGAATPSWSPETPANVTSQAAQAGQPSSIAFDLPAAPTGQTVY
                     RAVLVGTGPAPLVGLVDGAPVPLAGRVGGPSGSADDGHDVVLTIKEDTP"
     gene            3430..6879
                     /locus_tag="VEO00_07760"
     CDS             3430..6879
                     /locus_tag="VEO00_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57928.1"
                     /translation="MTATNGSGSAPAGFDGTVVLTQGVSGLVFVPSPTPLTHLNFYDG
                     KFLRAADLTLEQLGQRMLVELSNRAGGPGIAFGFDVSIGSNDVLSLSAGLAIDPQGRV
                     LLLPAAVSMSVATLIQESRGLAIPAITEGTGGSATFALCDPAIPPPTVQSQAGTELYL
                     LTIGHMEGLCGNEEVFGRLCDDNCVTADDRPYLLEGVVMRALPLRLSHALVTSTAVAL
                     GRVHLRSQVASAYFADEWDRGGTLLSSAGLAAATWCQGAAAMVGDAVPVGVLARAGTS
                     TMFLDEWTARRERIEPPPRRYWDGRMELRPWDVFLAQVLQFQCQLSEILSGGARGLPG
                     GGTDPCDDAHTLLDESVRLIKELGKRATAGKQIDPELFEKLPGLATFGSRSEAVLDRL
                     PVSSGRILIDGGIVELPPAGYLPVDPHSTVALSDQVRRLLGEGLDLRFCAVRRDQIAH
                     ELERAQHMNRISLLAGLDDPANKQNVDILVPDGTVSTVPAKTTGIGLDVDAAIGSTTR
                     SMVFNRSLSDSSRLVTDAATDPSALPIRGAARLTPTAAGGLQFVSAGSFVTGDRTAMI
                     GVMRTMARSATGAESFRNAVDRLTNLGDEVPESQPMEVLANEIHRSAAVARAGDEEIR
                     AVSLEQPAEGPQTAVMWVTFTTDRDPFTVSTGTVGVQASIDTYAPGRPALSVRQELIG
                     RLEILHSSETPTGGREVGVKFTASLDARHLGLRGIDEAPPSVSFLLTLTSETSAGLRR
                     IKVSTTGEKKVLFAFTAGWQGSPIEANARLVAEVQGEVAAGFGALALLHEDATITQQG
                     NEYHDIALQALIVLQGWILDDPGFLARAVQLLFPTQQGTGTSRVGATTDWVLFRRRRR
                     EDCGEVAVVPQPVGPSVVATWFTKAESLGEAGAIGSRLGSGELGDEVEWTRIGSAEFD
                     SGTATRLTSATELQQQWLNAGAGRAIGYAGYASTGGEPVAFAQPRAQALVAGVAQVAP
                     LDVGGKVEVVVNPPRAQMVAGTEGSVFFVTLEPPQHRVSCVEVFIVDDVNSSPEGKMR
                     LKALEQGDVVQLDDQAVQSKGRVTFTDDQPDLGQLQQVEDAVAEAQAEFAHGLFQQRF
                     PRVLAWVASSFDSDPDGPGAKMAIVVADRLGVPFFGPKVLNVDFDPGASCPVRLYVLE
                     FLG"
BASE COUNT         1017 a         2502 c         2581 g          936 t
ORIGIN      
        1 cctggggctt cgaccggcgc ctggcgatgg gcaaagggtt gaacgtcctg ttcgccgggc
       61 catccggcac cggcaagacg atggccgccg aggtgctggc ccatgagatc ggcctggacc
      121 tgtacaagat cgacctggcg acggtcatct ccaagtggat cggtgagacc gagaagaacc
      181 ttgcggcggc gttcgaggcg gccgaacgag gtggcatggc gctgctgttc gacgaggcgg
      241 acgccctgtt cggcaagcga acggaggtcg gcgacgcgcg ggaccgctac gcgaacctcg
      301 agaccgccta cctgctgtcc cggctcgagc ggttcgacgg actggcgatg ctcgcttcga
      361 acctccgcca gaatctcgac tccgcgttcg ctcggcgcct cgagttcatc gtccccttcg
      421 acctgcccga caccgaacag cgggtcgcgc tatggacgcg gcacatcccg cccggggcgc
      481 cgctcgcgtc cggcgtgcga ctggagcgcc tggccgccct ctacccgctg ccgggggcgc
      541 tgatccgcaa cgccgccgtc gccgctgcgt tcctcgccgc cgcggagggt tccgacatcg
      601 agccccgcca cctggtccac gccatcgagc gcgagtacgt gaaggccggg gatgccttcc
      661 ccgggccgcc accggaggac gcgcaatgac ccattccacc cgtcccgcag tcgaccgaag
      721 ggagtcgtcg tgaccaaccc cgtgaagcag ttccgcatcg acgagaaggc tcgccagggc
      781 atggcggtcc aggcggccca gaccgagctc gggcagatcg acggcgacct ggcgaccagc
      841 agggacacgg tggccgtgaa caccagggac ctcgccgccc agcaggccga ggagtcgcgc
      901 ctgcgccagg cgatcgccgc cgcggcgatg cccgccgacg cggatgcgct cgtcgaccag
      961 ctggaggaca acctgaagca gcagagcgtc agctcggccg cgctgaacga cgcgatcgat
     1021 caggccgcga cgctcgagcg gagccggctg cgcgtggcgg ccgagctcga cgccgcgagg
     1081 gccacccagg cccaggccgg cgcggacctc gccatcgcat ccgcggacga cgaggtggcc
     1141 gaacggtggc ggaccgcggc caccggcacc gcggtcggga atgcagtggc tgcggccggc
     1201 tcggcgagca cgacggcact gcgcacggcc gcccggacgc gactccagtc cctggcgggc
     1261 ggttccaccc tgctcgacct gttcaagcgc cgcttcgagg aggcgagagc cgactcggac
     1321 gagcgacgcg ccaaggtggt ccgggcgcag ggcgcgctcg acacggtggc gacgaagcag
     1381 gaccccgtga acggccccgt cgtggtcgcc ggtgcggggt acgcggcggc ccgcgaggtg
     1441 gtgcgggcga tcgccgagac cacggtccag cgcctcgacg cggccctcct cgcgctccag
     1501 acggcgagcg cgctgggcaa cctgacgacc gacgaacaga cccggatcga cgaccgcgcg
     1561 acccacgcca cggccgtgac cggcacgacg agctcggtga gcgcagcgag cgacgtgctg
     1621 gacgcccgga aggacgttcg tgtcgcggag gtggcgttcg acgtcgccgc gctcgccaag
     1681 gaggccacgg attcgacgtt cgaccgcgac acggacgcct cggtcaagac caagcgcgac
     1741 gcgtggaacg ccgccaaggc cgccctgtcg gcggcggaag gcaaggtgac gcccacccgc
     1801 cagcagcagc tggacgactg ggaggtggcc atccccgtcg gggtctcgag cgcggtcgtc
     1861 gggttcctcc gggcctcggc cgaggtggac gcgatcaagg gcctcgaccc ccagggccgc
     1921 gtgaacgacc tcaacgccgc cgagaccacg tacgcggggg cgctccagga ccggacggac
     1981 gccgcagagc tgcgcatggc gagggcgctg gaggtcgccg tacgactcga ggccgaggcg
     2041 gcggtcgaac cggtcgccga cgtgcgggag gccgccaagg tccgcggcga ccggtgagga
     2101 gcgaaccgtg acgacatcac tgggagaccg gccaccggcg gcgtcgctgg cggcggagcc
     2161 gtacacgtcg ctcgcggtcc actacgggat gttgctgggc gtcccggact tcgagctcat
     2221 gatgtcgaac ccgcgcggca agctccgcgt gcacaacgcg tggctgcacg gcgcgggcgt
     2281 cgtatgggga tacccggtgt ccgtcgacgc ccccagctgc gagctccggg tcgggcccgg
     2341 tctcgcggtc gacgggctgg ggcgggagct cccgctcacg atgctggcct gcctcgacgt
     2401 aggcgcctgg ttcgaccagc tcgacgacgc ggaccgggtg gccctcggga ccagcgagat
     2461 cgacgggggc ctccagttcc acgcgcacct cgtagctcgg gcggcgccgt gcctggcgag
     2521 gccggtcccg gcgatggggt cgaactgcga gggcggagcc tccgagaccg cgtactcgag
     2581 ggtgcaggag acggtggagc tcctgctcag ggccgggccc gcgccggacc cgccgccggc
     2641 ggcgttcccg tcgctccgcc gactgctcgc ggcaggcgac actggcgtcc cgggggaccg
     2701 ggagcgcatc gaggcgctgg cgacggcaga ccgcgagcgg gaatggctgg acgcgttcca
     2761 ccgcctggcc gccgacgagg tcgcggcgct gctccctccc ggcctcgtgg agggcggcgc
     2821 cggcagctcg accatgttcc cggtcgacga gccgggcgag gtcctgctcg ccgacctgcc
     2881 ggacctccag ctggtcacgc aggccgacgg cgcccggacg gtggcggcgc cgacgatcga
     2941 cctgtccgtc cgatcgagcc acgtgcccac gctcgtgctg gccgacctgc tcgcagagct
     3001 gctgacccgg ggcggcggcg cggccgacga cgccgacgcc ggcggcccgc gcgtggcctc
     3061 ggtgtcgcgc gcgggcatca cggtgaccgt cgagctcacg gcggacgtcg tcgacggcac
     3121 gctcgccgac gcgctgagcc tgcactcgat cgaccccggg gccgcgacgc cgtcctggtc
     3181 tcccgagacc ccggcgaacg tcacgtcgca ggcggcgcag gccggccagc cgtcctcgat
     3241 cgcgttcgac ctcccggccg cgcccaccgg tcagacggtg taccgggcgg tgctggtcgg
     3301 caccggcccg gccccgctcg tcgggctcgt cgacggcgcg cccgttccgc tcgccggccg
     3361 cgtcggcggc ccgtcgggtt cggccgacga cggccacgac gtcgtgctca cgatcaagga
     3421 ggacacgcca tgacggcgac gaacgggagc ggatcggccc ccgcggggtt cgacggaacg
     3481 gtcgtcctca cccagggcgt gagcgggctg gtgttcgtgc cgtcgccgac gccgctcacc
     3541 cacctgaact tctacgacgg gaagttcctg cgggcagccg acctgacgct cgagcagctc
     3601 gggcagcgga tgctcgtcga gctgtcgaac cgggcgggcg gcccggggat cgcgttcgga
     3661 ttcgacgtct cgatcgggtc gaacgacgtc ctctccctgt ccgcgggcct ggcgatcgac
     3721 ccgcaggggc gcgtgctgct cctgccggcc gccgtgagca tgtccgtcgc cacgctgatc
     3781 caggagagcc gcggactcgc gatccccgcc atcaccgagg gaacgggcgg ttcggcgacg
     3841 ttcgcgctgt gcgacccggc catcccgccg ccgacggtcc agtcccaggc ggggaccgag
     3901 ctgtacctcc tgacgatcgg ccacatggag gggctctgcg ggaacgagga ggtgttcggc
     3961 cggctgtgcg acgacaactg cgtcaccgcg gacgaccggc cctacctgct cgagggcgtg
     4021 gtgatgcggg ccctcccgct ccgcctgtcg cacgcgctgg tgacgtcgac ggccgtcgcg
     4081 ctcggccggg tccacctgcg gtcccaggtc gcatcggcgt acttcgccga cgaatgggac
     4141 cgcggaggaa cgctcctgtc ctcggccggc ctcgccgccg cgacgtggtg ccagggcgcg
     4201 gccgccatgg tcggggacgc cgtgcccgtc ggtgtgctgg cccgcgccgg gacctcgacc
     4261 atgttcctcg acgaatggac cgcgcggcgg gagcgcatcg agccgccgcc ccgcaggtat
     4321 tgggacggcc ggatggaact gcggccgtgg gacgtgttcc tggcccaggt cctgcagttc
     4381 cagtgccagc tctcggagat cctctcgggc ggggcgcgag ggctgcccgg tggggggacg
     4441 gatccctgcg acgacgccca cacgctgctc gacgagtcgg tccggctgat caaggagctc
     4501 ggcaagcggg ccaccgcggg gaagcagatc gacccggagc tgttcgagaa gctcccgggc
     4561 ctcgcgacgt tcggctcgcg gtcggaagcg gtgctggacc ggctgccggt ttcttccggg
     4621 cgcatcctca tcgacggggg catcgtcgag ttgcccccgg ccggctacct cccggtcgac
     4681 ccgcactcga cggtcgccct cagcgatcag gtccggcgcc tcctgggcga gggcctcgac
     4741 ctccggttct gcgcggtccg ccgggaccag atcgcccacg agctcgaacg ggcccagcac
     4801 atgaaccgta tctcgctgct ggccggactc gacgatccgg cgaacaagca gaacgtggac
     4861 atcctcgtgc ccgacggcac ggtctcgacg gtccccgcga agacgaccgg catcgggctc
     4921 gacgtggacg cggctatcgg ttcgacgacg cggagcatgg tgttcaaccg ctcgctttcc
     4981 gatagcagcc ggctcgtgac cgacgccgcg acggacccgt cggctctgcc gatccggggc
     5041 gcggctcggc tgacgccgac cgccgccggg gggttgcagt tcgtctcggc ggggtcgttc
     5101 gtgacgggcg accgaacggc gatgatcggg gtcatgcgga cgatggcccg gtccgccacg
     5161 ggagccgaat ccttccgcaa cgccgtcgac cgcctcacga acctcggaga cgaagtcccg
     5221 gaatcgcagc cgatggaggt gctggcgaac gagatccacc gcagtgccgc cgtcgcccgc
     5281 gcgggtgacg aggagattcg cgcggtctcc ctcgaacagc ccgccgaggg accgcagacc
     5341 gccgtcatgt gggtcacgtt caccaccgac cgggatcctt tcaccgtctc caccggcact
     5401 gtcggggtgc aggcctcgat cgacacctac gcccccgggc ggccggctct cagcgtccgc
     5461 caggagctga tcggccggct cgagatcctg cattcgagcg agacgcccac cggcggccgg
     5521 gaggtcggag tgaagttcac tgcgagcctc gacgctcggc acctcggcct ccgcggcatc
     5581 gacgaggccc cgccctcggt gagcttcctg ctcacgctca cctcggagac gtcagccggc
     5641 cttcgccgga tcaaggtctc gacgaccggc gagaagaagg tcctgttcgc attcaccgct
     5701 gggtggcagg gttcgccgat cgaggcgaac gctcggctgg tggccgaagt gcaaggggag
     5761 gtcgccgcgg ggttcggcgc gctcgcgttg ctccacgagg acgcgacgat cacccagcag
     5821 gggaacgagt accacgacat cgctctgcag gcgctgatcg tgctccaggg atggatcctg
     5881 gacgacccgg gcttccttgc ccgcgccgtg cagttgctgt tccccacgca gcagggaacc
     5941 ggaacctcca gggttggcgc gaccacggac tgggtgctgt tccggcggcg acgtcgcgag
     6001 gactgcggcg aggtggcggt cgtcccgcag ccggtcggcc cgagcgtcgt ggccacgtgg
     6061 ttcacgaagg cagagagcct tggcgaagcg ggcgctatcg gatcgcgtct cggcagcggc
     6121 gagttgggcg acgaggtcga gtggacccgg atcggttcgg ccgagttcga ttcgggaacg
     6181 gcgacccgac tcacgtccgc caccgagctc cagcagcaat ggctgaacgc cggagccggt
     6241 cgggccatcg ggtacgccgg ctacgcgtcg acgggcgggg agccggtggc attcgcccag
     6301 ccgcgggccc aagccctggt cgctggtgtg gcccaggtcg caccgctcga cgtaggtggg
     6361 aaggtcgaag tggtggtcaa cccgccccgc gcccagatgg tggcggggac ggagggcagc
     6421 gtcttcttcg tcacgttgga accgccgcag caccgtgtct cgtgcgtcga ggtcttcatc
     6481 gtcgacgacg tcaactcgtc gccggagggc aagatgcgcc tgaaggcgct cgagcagggc
     6541 gacgtcgtcc agctggacga ccaggccgtc caatcgaagg gccgcgtcac gttcaccgac
     6601 gatcagcccg acctgggcca gctgcaacag gtggaggacg ccgtcgcgga ggcccaagcc
     6661 gagttcgcac atggcctttt ccaacagcgg ttccccaggg ttctcgcctg ggtcgcgagc
     6721 agtttcgaca gcgatcccga cggacctggc gcgaagatgg cgatagtcgt ggccgaccgg
     6781 ctcggagtcc ccttcttcgg cccgaaggtg ctcaacgtcg acttcgaccc cggggcctcg
     6841 tgtcccgtcc ggctgtacgt cctcgagttc ctgggatgaa cacgtgacgg gcgcgagctc
     6901 ggcagggccg gcggccgaga agaaggagga ggcgggctcg aaggccccgg ccacggcggc
     6961 tgcgccggag agcggcaagg cggcgaccgg cacgacgtcg ccgggccccg cggcgggaag
     7021 ctcgctcggc gcgcgc
//
LOCUS       DASPWO010000338         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_642583, whole genome shotgun sequence.
ACCESSION   DASPWO010000338 DASPWO010000000
VERSION     DASPWO010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642583"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VEO00_07765"
     CDS             <1..189
                     /locus_tag="VEO00_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57929.1"
                     /translation="QLMLLGMRKLGDRRRAIRGGREPGAADPGTNSSGDRRGRRGRSA
                     RGARAAEPPPGRRRRGGR"
     gene            230..499
                     /locus_tag="VEO00_07770"
     CDS             230..499
                     /locus_tag="VEO00_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57930.1"
                     /translation="MRTRYRCSGCGNLTRFDVVSTRRTRAFHHFSVGGELTVEDEDVL
                     EEQVEDVTCRWCGGGRDLIETLPADDRPSDDRPSNDAAPTPRAAG"
     gene            complement(477..896)
                     /locus_tag="VEO00_07775"
     CDS             complement(477..896)
                     /locus_tag="VEO00_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HYU57931.1"
                     /translation="MQAAHEELTLETQGDGDILDVTSYAQKAIDNSGLADGLCTVFIA
                     HSTCGMTTIECEPGANADLNTVLERVAPQDQAWRHNELNADTNGHSHVRAALIGPSVV
                     IPFRAGELLVGTWQKIVCIDFDDRPRSRKLVIQLLGG"
     gene            1007..2446
                     /locus_tag="VEO00_07780"
     CDS             1007..2446
                     /locus_tag="VEO00_07780"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYU57932.1"
                     /translation="MTTSPQRVAVPALPGQLLIPLLDVAGDMLQALEPVDRPPALRRL
                     AEFDSRGLRTEAARQQLWRALEADEPFRERVVEEFRERSEVVAALEAWTAEEAFRHVG
                     DAADRSDLPLLASALYAARPRGWAFGLGAAAAIVDRQRLEQQEGEERKARATEIAALD
                     ESRRRLEEARDAAEAQAGKLQRELRDERHARRGRDADAERAVAEARGRAEQLEAELSR
                     ARAAAESTEGRIRREAERSRELEQELGAAKRALAESEQARAVAVEELERAAAPGSGLR
                     YADLQALTDAADLAERLASGLAGVAERARGVRRPEAPSRSDSGGVVAPADREPTRRLQ
                     PRVPPGMVADSPEALAAVLRSTRVVLVVDGYNVSMLGWGDAELVDQRERLSAALERLH
                     TRTRCDVTLVFDGAGIEGVRPARRPGVRTVFSAAGEEADRVVVREVGALSKKIPVVVA
                     SSDAEVRADAEREGALGVSSATLLSVLRS"
BASE COUNT          321 a          917 c          967 g          328 t
ORIGIN      
        1 cagctcatgc tgctcgggat gcgcaagctc ggcgatcgcc gccgcgcgat ccggggcggg
       61 cgtgagccgg gcgcggccga tcccggcacg aactcgtcgg gcgatcgccg cggccgcagg
      121 ggtcggtcgg cccggggcgc ccgggcggcg gaaccgcccc ccggccggag gcgccggggc
      181 ggccggtagc gaaccggccc tccaccggcc gattcgtaca ctggtcgccg tgcgcacccg
      241 ttaccgctgc tccggctgcg ggaacctcac ccgcttcgac gtcgtgtcca cccgacggac
      301 cagggccttc caccacttca gcgtgggcgg ggaactcacc gtcgaggacg aggacgtgct
      361 cgaggagcag gtcgaagacg tgacctgccg gtggtgcggg ggaggccggg acctgatcga
      421 gacgctccca gccgacgacc gcccctccga cgaccgccca tccaacgacg ccgcgcctac
      481 cccccgagca gctggatgac gagcttgcga ctgcggggac ggtcgtcgaa gtcgatgcac
      541 acgatcttct gccaggtgcc caccagcagc tcaccggcgc ggaaggggat gaccaccgac
      601 ggtccgatga gcgcggcgcg gacgtggctg tgcccgttgg tgtcggcgtt gagctcgttg
      661 tgacgccacg cctggtcctg gggtgcaacc cgctcgagga ccgtgttgag gtcggcgttc
      721 gcaccgggct cgcactcgat cgtcgtcatc ccgcaggtcg agtgggcgat gaacacggtg
      781 cagaggccgt cggcgagccc cgaattgtcg atcgccttct gggcgtagga ggtgacgtcg
      841 aggatgtcgc cgtcgccctg ggtctcgagc gtcagctctt cgtgcgctgc ctgcatccgg
      901 gtgctcccaa caggccggca tcgttgccgg ctcaccggcg cagtctacga gcccgccctt
      961 cccgcggcat ctcggcgcgt gcggtgagcg ggacggtgac aggccggtga ccacctcgcc
     1021 gcagcgggtc gcggtccccg cgcttcccgg gcagctgctc atccccctgc tcgacgtcgc
     1081 cggcgacatg ctccaggcgc tcgaaccggt ggaccgacca ccggcgctcc ggcggctcgc
     1141 cgagttcgat tcccgcggcc tccggaccga ggccgcccgc caacagctgt ggcgcgccct
     1201 cgaggccgat gagccgttcc gcgagcgcgt cgtcgaggag ttccgcgagc ggtccgaggt
     1261 cgtggccgcg ctcgaggcct ggacggccga ggaggcgttc cgccacgtcg gcgacgcggc
     1321 cgaccggtcc gatcttccgt tgctcgcctc ggcgctgtac gcagcgcgcc cgcgcggctg
     1381 ggcgttcggg ctgggtgccg ccgctgccat cgtcgaccgg cagcgcctcg agcagcagga
     1441 aggcgaggag cgcaaggcgc gcgccaccga gatcgccgcg ctcgacgagt cgcggcgccg
     1501 gctcgaggag gcgcgcgacg ccgccgaggc gcaggccggc aagttgcagc gcgaactccg
     1561 cgacgagcgc cacgcccggc gggggaggga cgccgatgcg gagcgggccg tcgccgaagc
     1621 ccgcgggcgc gccgagcagc tcgaggccga gctctcccgg gctcgggccg ccgccgagtc
     1681 gaccgaggga cgcatccggc gcgaagcgga gcggtcacgc gagctcgaac aggaactggg
     1741 ggcggccaag cgcgccctgg ccgagagtga acaggcgcga gccgtcgcgg tcgaggagct
     1801 cgagcgggcc gccgcacccg ggagtggcct gcgttacgcc gacctccagg cgctcaccga
     1861 cgccgccgac ctcgccgagc gcctggcgag cggtctcgcg ggagtggccg agcgggcccg
     1921 gggcgtccgc cggcccgagg cgcccagccg gagcgactcg ggtggcgtcg tcgcgccggc
     1981 cgatcgggag ccgacgcgcc ggctccagcc ccgagtgccg ccggggatgg tcgccgattc
     2041 gcccgaagcc ctcgccgcgg tgctgcgctc gacccgcgtc gtcctcgtgg tcgacggcta
     2101 caacgtgtcg atgctcggtt ggggcgacgc cgagctcgtc gaccagcgcg agcggctgag
     2161 cgcggccctg gaacgcctgc acacccggac ccggtgcgac gtgacgcttg tgttcgatgg
     2221 cgccgggatc gagggtgtgc ggccggcgcg tcgccccggc gtgcggaccg tgttctcggc
     2281 cgcgggcgaa gaggcggatc gagtcgtcgt ccgggaggtc ggcgccctct cgaagaagat
     2341 ccccgtggtc gtcgcgtcct ccgacgccga ggtgcgcgcc gacgccgagc gcgagggggc
     2401 gctcggcgtg tcgtcggcga cgcttctcag cgtgctgcgg agctgacgat caggtgcagc
     2461 aggcgctcgc gggcgccgac gttgccggct gcgtgtcggc gaggacggtg tagatctccc
     2521 agcgctcccc gtc
//
LOCUS       DASPWO010000339         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_981585, whole genome shotgun sequence.
ACCESSION   DASPWO010000339 DASPWO010000000
VERSION     DASPWO010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981585"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..1126)
                     /locus_tag="VEO00_07785"
     CDS             complement(338..1126)
                     /locus_tag="VEO00_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HYU57933.1"
                     /translation="MGVRRSRRTPAILLAAVAGAWALAIVAQTTGRAVLLDHGRLIED
                     APPLWHPPPLWVALPLFLIAWQVMVAAMMLPSSLPMIRLFRTVSIAQPRPEAVRAAFL
                     GGYLVVWTGFGAVAFLQDIGIHRLVDRTPWVGAHPWVIAGLALLLAGSFQFSALKDKC
                     LQECRHPGAFLLQYYRRGVPEAFRLGLRHGLFCLGCCWALMLVMFAAGVAQLWWMAAL
                     GALMFYEKVGRHGDRVTPIAGVALLALAVLVLVHPGWLPNVFHE"
     gene            complement(1135..1815)
                     /locus_tag="VEO00_07790"
     CDS             complement(1135..1815)
                     /locus_tag="VEO00_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HYU57934.1"
                     /translation="MADAKGVMAMAMTKEAAEQTSVVYDLQGTLLEACSCGVLCPCWV
                     GEDPDLGTCDAFIAYHFDAGTIGGVDVGGLSFVTVNHIPGNVLTPNSWRIVMFVDDRA
                     TDEQFQAIVDAYSGKLGGPLADLAGLVGEVLDVRRAPISHQIEGGAGTLTIGDFLHSE
                     MAPFTGADGSRTTLQNTVFSTVPGSPAYASKASVNSVNLPQYGMVWSFENRNAIQSDY
                     RITFAGEQ"
BASE COUNT          347 a          703 c          702 g          284 t
ORIGIN      
        1 gtcggcgcgc gctctctgtg gatgcctcga tcgcagcgga tcactcgacc gggccgtccg
       61 gtgatccgcc tggtgccgtc gttcgacccc tatctcctgg cgtaccggac ccgcgagttc
      121 tccgtggaca cgaggttcgc ccggcgtgtg catccgggtg gcggcgtcct gcacccgacc
      181 ctgctcgtgg acgggcgggc ggtcgccacg tggaggtcca ttcgccacgg cgccgggttg
      241 cacgtcgcga tcgagccgtt cacgtcactc gatcccgacg tcgaggaggc cgtggaggcc
      301 gaagctcgcg acgtggcgcg cttcctgggg ctcgagctca ctcgtggaag acgttcggca
      361 gccaccccgg atgcacgagg accaggacgg cgagcgcgag gagggcgacg cccgcgatcg
      421 gcgtcacccg atcaccgtgc cgtcccacct tctcgtagaa catgagcgcg ccgagggcgg
      481 ccatccacca cagctgagcg acgcccgcgg cgaacatgac gagcatgagc gcccagcagc
      541 accccaggca gaacagcccg tgccgcaggc ccaaacggaa ggcctcgggg accccacgcc
      601 ggtagtactg gaggaggaac gcgccgggat gccggcactc ctgcaggcac ttgtccttca
      661 gcgcggagaa ctggaaggag ccggccagga gcagcgccag ccccgcgatc acccacgggt
      721 gggcgccaac ccacggcgtg cgatcgacca gacggtggat gccgatgtcc tggaggaagg
      781 ccacggcccc gaatcctgtc cacaccacga gatatccacc caggaacgcg gcccggaccg
      841 cctccggacg aggctgcgcg atcgacaccg tgcggaacag gcgaatcatg ggcaggctcg
      901 agggcagcat catggccgcg accatgacct gccacgcgat gagaaagagc gggagggcca
      961 cccacagggg cggggggtgc cacaggggag gagcgtcctc gatcagtcgg ccgtgatcga
     1021 gcaggactgc ccttcccgtg gtctgcgcca cgatggccag cgcccaggcg cccgcgacgg
     1081 cggcgaggag gatggcgggg gtccgccgag aacggcggac ccccatggtc gagctcactg
     1141 ctcccccgcg aaggtgatcc ggtagtcgga ctggatcgcg ttgcggttct cgaacgacca
     1201 caccatcccg tactgcggga ggttcaccga gttgacggag gccttcgagg cgtacgcggg
     1261 cgagccgggg acggtcgaga agacggtgtt ctggagcgtc gtccgcgagc cgtcggctcc
     1321 ggtgaacggg gccatctcgg agtggaggaa gtccccgatg gtgagggttc ccgcgccgcc
     1381 ttcgatctgg tgagagatgg gcgcccgccg gacgtccagc acctccccga ccaggcccgc
     1441 caggtcggcc agggggccgc ccagcttgcc cgagtacgcg tcgacgatcg cctggaactg
     1501 ctcgtccgtc gcccgatcgt cgacgaacat cacgatcctc cacgagttcg gcgtgagcac
     1561 gttcccgggg atgtggttca ccgtcacgaa cgagagccct ccgacgtcga cgccgccgat
     1621 cgtccccgcg tcgaagtggt acgcgatgaa cgcgtcacac gtccccaggt ccgggtcctc
     1681 gcccacccag cacgggcaca gcaccccaca cgagcacgcc tccaacagcg tgccctggag
     1741 gtcgtacacg accgaggttt gctcggccgc ctccttcgtc atcgccatgg ccatcactcc
     1801 tttcgcgtcg gccaccgacg acggcgatgc tcgtgcttca tgtcgcagcc ctgttgcaac
     1861 ggcactgcaa cacgcgctgc aataacagcg gcatgacggc accggggagg ggacggacgt
     1921 gagggcgcgc tatccggacg tcgacggcta cgtggaacgc gacggcgtca aggtgttcta
     1981 cgaggtcttc gggtcgggag agccgacggt cctgctgatg ccgacgtggt ccatca
//
LOCUS       DASPWO010000340         4692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_981658, whole genome shotgun sequence.
ACCESSION   DASPWO010000340 DASPWO010000000
VERSION     DASPWO010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981658"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3558)
                     /locus_tag="VEO00_07795"
     CDS             complement(<1..3558)
                     /locus_tag="VEO00_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYU57935.1"
                     /translation="MRRRVVIALCVAATAFSAPPALADFPYARPGANTQDYDDLYIGD
                     DTPDDLSGDGNDWKFAATNDPANVTVNSNPVELDGVRGAHVADEVDAPSTAWETTTGR
                     PDVTIAVLDSGIKWNEADTMADLAEKVRLNKGELPLPQHANGTDCAAYDCNADGVFNL
                     TDYQDDPRVLDVLNNDPRRAGPPGVLTPEDVIIAFSDPGFTSPGRAGGQDDDGNGYVD
                     DVAGWDFLDDDNDAYDDVQYGHGTGEAKDSSAEANNDAEGDQAGACPNCTFIPLRVGD
                     SFIADVNRFAAAVLYATDNGVSVVQEALGTLNNSSFARQAVDYAYRHGVTVIASAADE
                     AAQHNNWPSSLPHVILVNSVRDEDLAAPANSYLAINGCTNFNSKITLAIPSSSCSSNA
                     TGLGAGMAGLVYSAALNALDNGALSGYPDDSACELTNGDPCPITPNEVRQIMASGTID
                     GTLQADDVNFAGTPPGSGDEPACSPLPLPGCTSPYGPGDLLKAQVDSNRPSHLAGPVA
                     TTSYPARQGHDQFYGWGRVNMDRAVDALVADPANPGATPSEIPPEAEITSPQWYEQVD
                     PSNLTLAVGGEVFARGAPYVCDVLVAPGQYPNQSETTDTPPGDFESVGSGYCDGTTAH
                     SGQDAALLHSGALAEISLSDLESRFPPGTDFNGAEPEAGPTTGNGRPNFAPHSFTVKL
                     VVSTTQGTPMTGEDQRSAYLHRDQDMLDGFPRAIRSGQVVGPPGTPTGDGASSPVFAD
                     LDGDNRNELILGGSDGFVHALRPDGSELPGWPVRADAQGFVASHSGALAYQSGGVSTD
                     IGGAILASIAVGDANRDGLPEVYAADLEGKVYGWGPTGERVFTEEANPAYSGKPLQPF
                     VNIRKGKTNRTQHGFIGSPVLADLDGDGRQELVAAGMDRHLYAWSANDGNPDAPGGAA
                     DVSGFPVLVVDPEKVAAVNSQTHRVAFVPGSFQQGAIVDTPAVADITGGADPEIIVGT
                     NEEYDETPNAGNFTTATFDPISGTGVVDPGNGRLFAIDATGDHDGDPKPTDALVNGWP
                     VRIGILLTELLPVVGEGITGSPVVAPVACPSGGSGPKVGVIPDAGPGYILNSNGTSCY
                     GNAPDGRYNALESDAFASAEADHPAIPAVGHPAFGQVVPGGDPTFLAPAAGLVRSLDV
                     GLPDYQGGQDFIGAWTASSGQFQPSFPQAVNDLQFLTGPS"
     gene            complement(3560..>4692)
                     /locus_tag="VEO00_07800"
     CDS             complement(3560..>4692)
                     /locus_tag="VEO00_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57936.1"
                     /translation="VLKGGITLNGLVNVGVATGQNLPYNHVVQAWNGQSGASLPSYPQ
                     AVEDYQLLSSPAVADLSNAPGVEATVGTGLYLIRNMNTAGVEGAGWPKFTGGWNFAVP
                     AIGDVDRDGMLEVSTLTREGFSFLWDTDSPACGTNDEWWTSRHDEWSTGAYGTDTRPP
                     GTPEDLTASRDGSAVTLSWTAPGDDWLCGTAEKLRVIGSPNAIQHPSDGTQIREEDVT
                     VGPGQGATLDLSDAELAGLGHVAILYRDDAGNWGHLADVEVPAQAGGGGGGGEPTPQN
                     PAAGPCSNVISGTSGPDHLDGTDGPDKISGKGGPDKIKGGPGDDCLRGGRGPDKISGG
                     DGDDTIHVRGGRTDKVNCGPGDDTVTASASDHVSHSCETVKA"
BASE COUNT          617 a         1580 c         1712 g          783 t
ORIGIN      
        1 cgagggaccg gtgaggaatt ggagatcgtt gaccgcctgc gggaagctgg gctggaactg
       61 accgctggac gcggtccagg cgccgatgaa gtcctgccct ccctgatagt caggcaggcc
      121 gacgtcgagc gaccgcacga gccccgcggc gggggcgagg aaagtgggat cgccgccggg
      181 tacgacctga ccgaacgcgg gatgcccgac ggcgggtatc gccggatgat cggcctccgc
      241 gctcgcgaag gcgtccgact cgagcgcgtt gtagcggccg tccggggcgt tgccgtagca
      301 cgaggtcccg ttcgagttga ggatgtagcc cggaccggcg tcagggatca ccccgacctt
      361 gggccccgat cctccgctcg ggcaggcgac cggcgcgacc acgggggatc cggtgatgcc
      421 ctcgccgacg acggggagaa gctcggtcag caggatccct atcctcaccg gccagccgtt
      481 gacgagggcg tcggtgggct tcggatcgcc gtcgtgatcg ccggtcgcgt cgatcgcgaa
      541 gaggcgcccg ttgccagggt ccacgacccc cgtgcccgag atcgggtcga acgtcgccgt
      601 ggtgaagttc ccggcgttcg gcgtctcgtc gtactcctcg ttggtgccga cgatgatctc
      661 cgggtcggcg ccgcctgtga tgtcggcgac cgcaggggtg tcgacgatcg ccccctgctg
      721 gaaggagccc gggacgaagg cgacgcggtg ggtctggctg ttgacggctg ccaccttttc
      781 cggatcgaca acgagcaccg ggaaccccga gacatcggcc gcaccaccgg gcgcatccgg
      841 gtttccgtcg ttcgcgctcc aggcatagag gtgtcggtcc atcccggcag cgaccagctc
      901 ctgccttccg tccccgtcga ggtccgccag aaccggtgag ccgatgaacc cgtgctgggt
      961 gcggttggtt ttgcccttgc ggatgttgac gaacggctgc agcggtttgc cggagtaggc
     1021 gggattcgcc tcctcggtga agacccgctc accggtcggc ccccagccgt agaccttgcc
     1081 ctccaggtcc gcggcgtaga cctccggcag gccgtcgcga ttggcgtcgc ccacggcgat
     1141 cgatgccagg atcgccccgc cgatgtcggt cgacacaccg ccgctctggt atgcgagcgc
     1201 tccggagtgg ctcgcgacga atccctgggc gtcggcgcga accggccagc cgggcagctc
     1261 tgatccatcg gggcgcaggg cgtggacgaa tccgtcgctg ccgccgagga tcagctcgtt
     1321 gcggttgtct ccgtcgaggt cggcgaagac gggcgaggac gcgccgtcgc cggtcggagt
     1381 tcccggtggg ccgactacct gcccgctgcg gatcgcccgc gggaagccgt cgagcatgtc
     1441 ctgatcgcgg tgcaggtagg ccgatcgctg gtcctcgccg gtcatcggcg tgccctgcgt
     1501 cgtcgagacg acgagcttga cggtgaacga atgcggcgcg aagttggggc ggccattgcc
     1561 cgttgtcggg cccgcctcgg gctctgcgcc gttgaaatcg gttccgggcg ggaagcggga
     1621 ctccagatcg ctgagcgaga tctcggcgag cgcccccgag tgcagcaggg cagcgtcctg
     1681 cccgctgtgg gcggtcgtgc cgtcgcagta gccgcttccg acggactcga agtcgcctgg
     1741 cggggtgtcg gtcgtttcgc tctgattcgg gtactgcccc ggggcgacga gcacgtcgca
     1801 tacgtacggg gcgccgcgcg cgaacacctc gcccccgacg gccagcgtca ggttcgaggg
     1861 atcgacctgc tcgtaccact ggggtgaggt gatctcggcc tcgggcggga tctccgaggg
     1921 ggtggcgccc ggattcgccg ggtcggcgac gagcgcgtcc accgccctgt ccatgttcac
     1981 cctgccccag ccgtagaact gatcgtggcc ctggcgcgcg gggtagctcg tcgtcgccac
     2041 gggaccggcg agatgtgagg gccggttgga atcgacctgg gccttgagca gatcgccggg
     2101 cccgtacggg ctcgtacacc ccggcagcgg cagtggactg cacgcaggct cgtcgcccga
     2161 ccccggcggc gtcccggcga agttgacgtc gtcggcttga agcgtcccgt cgatcgtccc
     2221 cgaggccatg atctggcgga cctcgttcgg ggtgatcggg cacggatcgc cattggtcaa
     2281 ctcgcaggcg gagtcatccg gatagccgga gagagcaccg ttgtcgagcg cgttgagcgc
     2341 cgcggagtag acgagcccgg ccatccccgc gccgagcccg gtggcgttcg aggagcaact
     2401 cgagctcggg atcgcgaggg tgatcttcga gttgaagttg gtgcagccgt tgatcgccag
     2461 atatgagttc gcgggggccg cgagatcctc gtcccggacc gagttgacga gaatcacgtg
     2521 cggcaggctc gacggccagt tgttgtgctg ggccgcctcg tcggcggccg acgcgatcac
     2581 ggtgacgccg tggcggtagg cgtagtcgac ggcctgacgg gcgaagctcg agttgttgag
     2641 cgtcccgagc gcctcctgca cgacgctgac gccgttgtcg gtcgcgtaga ggacggcggc
     2701 ggcgaagcgg ttgacgtcgg cgatgaaact gtcgccgacg cggagcggga tgaaggtgca
     2761 gttcgggcac gccccggcct gatcgccctc ggcgtcgtta ttggcctccg cggatgagtc
     2821 cttggcctcc ccggtcccgt gcccgtactg gacgtcgtcg taggcgtcgt tatcgtcgtc
     2881 gaggaagtcc cagccggcga cgtcgtcgac gtagccgttg ccgtcgtcgt cctgaccgcc
     2941 ggcgcgcccc ggcgacgtga agcccgggtc cgagaacgcg atgatcacgt cctcgggagt
     3001 gagcaccccc ggcgggccgg cgcgccgcgg gtcgttgttc agcacgtcga ggacgcgcgg
     3061 gtcgtcctgg tagtcggtga ggttgaagac cccgtcggcg ttgcagtcgt atgcggcgca
     3121 atcggtcccg ttggcgtgct gcggcagcgg cagctctccc ttgttgagcc ggaccttctc
     3181 cgcgaggtcg gccatcgtgt cggcctcgtt ccacttgatc ccagagtcga gcacggcgat
     3241 cgtcacgtcc ggacggccgg tggtcgtctc ccaggcggtg gagggcgcgt cgacctcgtc
     3301 ggcgacgtgg gcgccgcgca ctccatccag ctcgacgggg ttcgagttga cggtcacgtt
     3361 cgcgggatcg ttggtcgccg cgaacttcca gtcgtttccg tcgccggaca ggtcgtcggg
     3421 ggtgtcgtcc cctatgtaaa ggtcgtcgta gtcctgggta ttggcgccgg gccgcgcgta
     3481 ggggaagtcg gcaagcgccg ggggagcgga gaacgccgtc gccgcgacgc agagcgcgat
     3541 gacgacccgg cggcgcaacc taggccttga ccgtctcgca gctgtgactg acatggtcgc
     3601 tggcgctggc ggtcaccgtg tcgtcgccgg ggccgcagtt gaccttgtcg gtgcgcccgc
     3661 cgcgcacgtg gatcgtgtcg tcgccgtcgc cgccgcttat cttgtcgggc ccgcgcccgc
     3721 cacgcaggca gtcgtcgcca ggtccgccct tgatcttgtc cgggccgccc ttgccgctga
     3781 tcttgtccgg gccgtccgtg ccgtcgaggt ggtcggggcc ggacgtcccc gagatcacgt
     3841 tcgagcatgg accggccgcc gggttctggg gagtcggctc gccgccgcct ccgccgcccc
     3901 cggcctgcgc cgggacctcg acgtccgcga ggtggcccca gttgcccgcg tcgtcgcggt
     3961 agaggatcgc gacgtggccc aagcccgcca gctcggcgtc cgagagatcg agcgtcgccc
     4021 cctgccccgg gccgaccgtc acgtcctcct cgcggatctg ggtgccgtcg gagggatgct
     4081 ggatcgcgtt cggcgacccg atcacgcgca gcttctcggc cgtgccgcac agccagtcgt
     4141 cgccgggcgc cgtccagctc agggtcaccg ccgagccgtc gcgggaggcg gtgaggtcct
     4201 ccggcgtgcc cggcgggcgt gtgtcggtcc cgtaggcgcc ggtcgaccac tcgtcatgac
     4261 gcgaggtcca ccactcgtcg ttggtgccgc acgccgggct gtcggtgtcc cacaggaacg
     4321 agaagccctc ccgggtgagg gtcgagacct cgagcatccc gtcgcggtcg acgtccccga
     4381 tcgccggcac ggcgaagttc cagccgccgg tgaacttcgg ccaccccgcg ccctcgacgc
     4441 cggccgtgtt catgttgcgg atcaggtaga gcccggtgcc aacagtggcc tctacccccg
     4501 gcgcgttcga gaggtccgcg accgccgggc tggacagaag ctggtagtcc tccaccgcct
     4561 gcgggtagga gggcagcgag gcgccggact gcccgttcca cgcttggacg acgtggttgt
     4621 aggggaggtt ctggcccgtg gccaccccca cgttcaccag cccgttgagg gtgatgcccc
     4681 ccttcagcac ct
//
LOCUS       DASPWO010000341         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_221214, whole genome shotgun sequence.
ACCESSION   DASPWO010000341 DASPWO010000000
VERSION     DASPWO010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221214"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2066
                     /locus_tag="VEO00_07805"
     CDS             <1..2066
                     /locus_tag="VEO00_07805"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYU57937.1"
                     /translation="VDQFEELFRFRDAAGATARDESSAFVKLLLEATRQRELPIYVVL
                     TMRSDFVGECAQFRDLPEALNEGQYLVPRMTRDQRREAIVGPAAVGQGRVSPRLVQRL
                     LNDVGDDPDQLPILQHALMRTWTHWEDHGAGRDLDVEDYEAIGGMADALSQHADEAYG
                     ELPDDGKRLAGRLFKCLTTRGANAQPIRRPTTVARLAAVAGAPVADVRAVIERFRVPG
                     RSFLMPPVPTALDDTAVVDISHESLIRQWNRLRAWADEEAESRALYLRIVDAAVRHER
                     GLGGLWRDPDLKLALDWKSANAPNPAWAALYDPGFQGAMRFLTESQADVEAEWRKRTR
                     QRRWLAAAVGAALLLTLVAIMALVARTASENEKRTADAARDVAVRQSAIAVSERVEAE
                     KQRGEAEKQRAEAETQRQQAQAARAAAEEQRQKAEAALATAEQQRRLAQERLREVERA
                     QLAAKQEADRAAKESARAERATVTVARQQRALADVFSGVRPSVVKLGSITPAGPQFMA
                     TAFFASTSGLLVTAAHVAAGLRDEGVPLRRTDGSTITGRKVRVDTGRDLALFAAPSDR
                     STACLPLFDGEVQPGAQVIALGMSPTQEWLATSGTVTATRARLSIPGVPAADDLIQVD
                     MKTEPGFSGAPVLDVLTRRVVGIVGYGRTDSGVHFLIPASRIRAAFGRELALAPCPVS
                     LGIAG"
     gene            complement(2178..>3139)
                     /locus_tag="VEO00_07810"
     CDS             complement(2178..>3139)
                     /locus_tag="VEO00_07810"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HYU57938.1"
                     /translation="ADLYGQYFFGDFCSGRIWTATDASGSWVATEALSSSLSISSFGE
                     DEAGELYVADRADDTGAVYRIVSAGASGPPDLVIEALAVSPGTVEAGGTVQVSYRVAN
                     RGTGTAAGGFREKILLSSDATLGNDSLRGTSPIHPDPLPPGGTLNLSQNVVVMADTTP
                     GDYFILVRADAFGVDAESNEANNVAAVPLRVQPGTTKDLVIRKLQLSAGSVRAGGTVV
                     VSYDVVNLGAATITESYRERIFLSTNHSVGGDALIGTSHVHTDHAPTLGPNGHHPSSS
                     IVTIPAGTAPGAYFILVQADTFNAVTETRETNNVTPVSITVTP"
BASE COUNT          460 a         1112 c         1134 g          433 t
ORIGIN      
        1 tcgtcgacca gttcgaggag ctgttccggt tccgcgacgc ggcaggggcc acggcgaggg
       61 acgagtcatc cgccttcgtg aagctgctgc tggaggcgac gcgccagcga gagctcccga
      121 tctacgtcgt cctgacgatg cgctcggact tcgtcggcga gtgcgcgcag ttccgcgacc
      181 tccccgaggc gctcaacgag ggacagtacc tcgtcccccg catgacccgg gaccagcggc
      241 gcgaggccat cgtcggcccg gccgccgtcg gccaggggcg cgtgagcccc cgcctcgtgc
      301 agcgtctcct gaacgacgtc ggcgacgatc ctgatcagct cccgatcctg cagcacgccc
      361 tcatgcggac gtggacgcac tgggaggatc acggggccgg ccgggacctc gacgtcgagg
      421 actacgaggc catcggcggg atggccgacg cgctgtcgca gcacgccgac gaggcctacg
      481 gtgagctgcc ggacgacggc aagcgtctcg ccgggcggct cttcaagtgc ctcacgacgc
      541 ggggagcgaa cgcccagccg atccgacgac cgaccacggt ggcccggctc gccgcagtcg
      601 ccggcgcccc ggtggccgac gtccgcgcgg tcatcgagcg attccgcgtg cccggccgat
      661 ccttcctgat gcccccggtc ccgacggcgc tcgacgacac cgcggtggtg gacatctccc
      721 acgagagcct gatccggcag tggaaccgcc tgcgtgcctg ggccgacgag gaagcggagt
      781 cccgcgccct ctacctccgg atcgtcgacg cggcggtgcg ccacgagcgc gggctcggcg
      841 gactgtggcg cgatcccgac ctgaagctgg cactcgactg gaagtccgcg aacgcgccga
      901 accccgcgtg ggcggccctc tacgatcccg gcttccaggg cgcgatgcgg ttcctcaccg
      961 agagccaggc cgacgtggag gccgagtggc gcaagcgcac gcgccagaga cggtggctcg
     1021 cggccgcggt cggcgccgcc ctgctgttga cgctcgtcgc gatcatggcc ctggtcgcga
     1081 ggacggcatc ggagaacgag aagcgcacgg ccgacgccgc gcgtgacgtc gcggtcaggc
     1141 agagcgccat cgcggtctcg gagcgcgtcg aggccgagaa gcagcggggc gaagccgaga
     1201 agcagcgcgc cgaggccgag acgcagcggc aacaggcgca agccgcccgc gctgccgcgg
     1261 aagagcagcg gcaaaaggcc gaggcggccc tcgcgaccgc ggaacagcag cgccgcctgg
     1321 cccaggagcg cctgcgggag gtggagcgcg cgcagctcgc ggcgaagcag gaagccgacc
     1381 gggcggccaa ggagagcgcg cgagccgagc gggcgaccgt caccgtcgcc cggcaacagc
     1441 gcgcgctcgc cgacgtcttc tcgggcgtcc gcccgagcgt cgtgaagctg ggctccatca
     1501 ctccggcggg tccacagttc atggccacgg cgttcttcgc gtctacctcc gggctgctgg
     1561 tcaccgccgc tcacgtggcg gcagggctgc gagacgaggg cgtgccgctg cggcgaaccg
     1621 atggctccac gatcaccggc aggaaggtcc gggtggacac cggacgcgat cttgcgctgt
     1681 tcgcggcgcc gtcggaccgc tccacggcct gcctcccgct cttcgacggc gaggtccagc
     1741 ccggcgcgca ggtcatcgcc ctcggcatgt cgccgacgca ggagtggctg gcgacgagcg
     1801 ggacggtgac agcgacgcgc gcacgtctga gcattcctgg tgtgccggcc gccgatgacc
     1861 tgatccaggt ggacatgaag accgagccgg ggttcagcgg cgcgccggtc ctcgacgtcc
     1921 tcaccaggcg cgtcgtcggc attgtcggct acggccggac ggactcgggg gttcacttcc
     1981 tcatccccgc ctcgaggatc cgggcggcgt tcggtcggga gctggcgttg gcgccgtgcc
     2041 cggtcagcct gggcatcgcg ggataggcac ggcgaggcat tttgcgcttg ccgagggtcc
     2101 cggctggtgc ctgattaccc cgaactcgcg cgatcccctc cctcctcgag gtgctcgagc
     2161 cgagaccccc tcccaactta cggcgtgacg gtgatggaca ccggcgtcac gttgttggtc
     2221 tccctggtct cggtcaccgc gttgaaggtg tcggcctgca cgaggatgaa gtacgccccg
     2281 ggcgccgtcc ccgccgggat cgtcacgatc gaggagctgg gatggtggcc gttcggaccc
     2341 agcgtcggcg cgtgatccgt gtggacgtgg ctcgtcccga tcagggcgtc gccgccgacg
     2401 gaatggttcg tcgagaggaa gatccgctcc cgatagctct cggtgatcgt cgccgccccg
     2461 aggttgacca cgtcgtagct cacgaccacc gtcccgcccg cgcgcacgga gccggccgag
     2521 agctggagct tcctgatcac gagatccttg gtcgtccccg gctggacccg cagcgggacg
     2581 gcggcgacgt tgttggcctc gttgctctcc gcgtccacgc cgaaggcgtc cgcccgcacc
     2641 aggatgaagt agtcgccggg cgtcgtgtcg gccatcacca ccacgttctg gctgaggttg
     2701 agcgtgccac cgggcgggag cgggtccgga tggatggggc tcgtgccccg tagcgagtca
     2761 ttgccgagcg tggcgtccga ggagaggagg atcttctccc gaaagccacc ggccgccgtg
     2821 ccggtcccgc ggttggcgac gcgatacgag acctgcacgg tgccgcccgc ctcgacggtc
     2881 cccggcgaga cggccagcgc ctcgatcacg agatcgggcg gcccggaggc cccggccgac
     2941 acgatgcgat acacggcgcc ggtgtcgtcg gcgcggtccg ccacgtacag ctcgccggcc
     3001 tcgtcctcgc cgaagctgct gatcgacagg gagctggaga gcgcctcggt cgccacccag
     3061 cttcccgacg cgtcggtggc ggtccagatc cgccccgagc agaagtcgcc gaagaagtac
     3121 tggccgtaga gatcggcaa
//
LOCUS       DASPWO010000342         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1071383, whole genome shotgun sequence.
ACCESSION   DASPWO010000342 DASPWO010000000
VERSION     DASPWO010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071383"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VEO00_07815"
     CDS             complement(<1..314)
                     /locus_tag="VEO00_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYU57939.1"
                     /translation="MRVTVDDDVRLEVREAGAGPALLLVHGFGGAKEDFADHIEALGA
                     RYRVVTFDHRGHGESDAPDHVEAYSLDRLAADTLQVADALGIDRFRLLGHSMGGMVAR
                     RLV"
     gene            377..1384
                     /locus_tag="VEO00_07820"
     CDS             377..1384
                     /locus_tag="VEO00_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HYU57940.1"
                     /translation="MAVTETAAARPAAWKLWLRVIVSAALLAFLVSKIHLGTLLPEHP
                     HLSTLFFFVAGIAMAGLGIVLSAWRWQRALVVFDAHVPLPTLVSHYFAGQFFGNVLPS
                     TIGGDVLRVRWGAKTTGSGHVSFGSVVLERLTGFVALPLLALLGFALRPSLLDEDHAW
                     IAVVISAVTLVMLVVILYAAAHPRLAGRFRDHENWMRFVGAVHVGVDRLRRQPRLAAG
                     VLGAAILYQASTVAVVYCAVRTLGVELPTAAVLAFAPAVAMAQVLPLSLSGLGVREGM
                     LVILLHPLGVSSGRAVAIGLLWYAMMLIVSLFGAPAFAVGHRHRPANDQPVNHRPADS
                     P"
     gene            1381..2178
                     /locus_tag="VEO00_07825"
     CDS             1381..2178
                     /locus_tag="VEO00_07825"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYU57941.1"
                     /translation="MSLADETATPARRPFRLPGGRELRDGRHLYWWVEILAIIAFYMI
                     YSAIRNANEGGTADAYANARDLISWQRSLGINHEEWLQDWALHFRPLIIAMNYVYGSL
                     HFVITAGVGIYLFRKWSNDYPQWRNTLAITTGLALVGFILWPLMPPRLLPESYGFVDT
                     LAKYPTFWSFNSGAVSKISNQYAAMPSVHCAWALWCACVAVPRVRAHWAKVLFALYPA
                     LTVTAIVLTGNHYVLDAVGGFAVLGIGYALARTFTRAGRGPALQTTG"
     gene            2209..3060
                     /locus_tag="VEO00_07830"
     CDS             2209..3060
                     /locus_tag="VEO00_07830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYU57942.1"
                     /translation="MTRHVRLREFLVGVEGLALFRQLVEGDDADAQARIDEVRRIVGT
                     DEEAVYGLGMDIPELNARAGYAKWSETYDEPGNPLITVEEPVVQELVGAAPPGLALDA
                     ACGTGRHSRFLAENGHDVIGVDGSPEMLARAAVHVGSGTFVQGDLEALPLRSAGIDLA
                     VCALALGHLVELAPAVAELARVVRPGGRIVLSDLHPTMSATGGQALFRAADGSSAFVR
                     EHPHVHSEYLAAFAAADLEVRACVEPRFDETAAVMQGPAHDFVPEATVAAFRGLPGAL
                     VWDLARR"
BASE COUNT          387 a         1135 c         1058 g          496 t
ORIGIN      
        1 acgaggcggc gggcgaccat gccgcccatc gagtggccca gcagccggaa gcggtcgatg
       61 ccgagcgcgt cggccacctg gagggtgtcg gcagccagcc ggtcgagcga gtacgcctcg
      121 acgtgatcgg gagcgtcgct ctcgccgtgg ccccggtggt cgaacgtcac cacccggtac
      181 cgggcgccga gcgcctcgat gtggtcggcg aagtcctcct tggcgccacc gaacccgtgg
      241 acgaggagga gggcaggacc ggcgcccgct tcgcgtactt cgagccggac gtcgtcgtcg
      301 accgtgaccc tcaccggtgc ctcctcggag cgcggctgct gccgccgcgg caaagcctcg
      361 ccagtaggct acgacgatgg cggtgaccga gacggctgcg gcgcgcccgg cggcctggaa
      421 gctgtggttg cgggtcatcg tcagcgccgc gctgctcgcc ttcctcgtgt cgaagatcca
      481 cctcggcacg cttctgcccg agcacccgca cctctcgacc ctgttcttct tcgtcgccgg
      541 catcgcgatg gcggggctcg ggatcgtgct gtcggcctgg cggtggcaac gcgcgctggt
      601 ggtcttcgac gcccacgtcc cgcttccgac gctcgtgtcc cactacttcg cgggccagtt
      661 cttcggcaac gtcctgccct cgacgatcgg cggcgacgtc ctgcgggtca ggtggggcgc
      721 gaagacgacc ggatcgggac acgtgtcgtt cggatcggtc gtgctggagc ggctcaccgg
      781 cttcgtcgcc ctgccgctcc tggcgctcct cggcttcgcg ttgcgcccgt ctctcctcga
      841 cgaggatcac gcctggatcg cggtggtcat ctccgcggtg acgctcgtca tgttggtggt
      901 catcctctac gcggccgccc atccccgcct ggcgggacgc ttccgcgacc acgagaactg
      961 gatgcggttc gtgggcgcgg tgcacgtcgg ggtcgaccgc ctgcggcgcc aaccccggct
     1021 cgccgccggc gtgctgggcg cggcgatcct gtaccaggcc tcgaccgtcg cggtcgtcta
     1081 ctgcgcggta cgcacgctgg gggtcgagct gccgaccgcg gccgtgctcg cgttcgcccc
     1141 cgccgtcgcc atggctcagg tgttaccgct gtcgttgagc ggcctcgggg tccgggaggg
     1201 gatgctcgtc atcctgctcc acccgctcgg ggtctccagc gggcgggcgg tcgccatcgg
     1261 gttgctctgg tacgccatga tgctgattgt gagcctgttc ggcgcgcccg ccttcgcagt
     1321 cggtcaccgt caccggccgg ctaacgacca gcccgtcaac caccgccccg ccgacagccc
     1381 gtgagcctcg ccgacgagac tgcgacgccc gcgcgccgcc cgttccggct gccgggcggg
     1441 cgcgagctgc gcgacggccg tcacctctac tggtgggtcg agatcctggc gatcatcgcc
     1501 ttctacatga tctattcggc catccggaac gcgaacgagg gtgggacggc cgacgcctac
     1561 gccaacgccc gcgatctcat cagctggcag cggtcactcg gcatcaacca cgaggagtgg
     1621 ctccaggact gggcgcttca cttccggccg ctcatcatcg cgatgaacta cgtctacgga
     1681 tcgctgcact tcgtcatcac cgctggggtc gggatctacc tgttccggaa gtggagcaac
     1741 gactacccgc agtggcgcaa cacgctcgcg atcaccaccg ggctggcgct ggtgggcttc
     1801 atcctctggc cgctgatgcc accccggctg ctacccgaga gctacggctt cgtcgacacc
     1861 ctggcgaagt acccgacctt ctggtcgttc aactccggcg cggtgagcaa gatctccaac
     1921 cagtacgccg ccatgccgag cgtccactgc gcctgggcgc tgtggtgcgc gtgcgtggcc
     1981 gtcccgcggg tacgggcgca ttgggccaag gtgctgttcg cgctgtatcc cgccctgacc
     2041 gtcaccgcga tcgtcctcac cggcaaccac tacgtgctcg acgcggtcgg cgggttcgcc
     2101 gtcctcggga tcggttacgc gctggcgcgg acgttcaccc gcgccgggcg cggtcccgcg
     2161 ctgcaaacca ccggctgagc cgaccggccg ctcgatagcg tgcgacccgt gacccgacac
     2221 gtccggctgc gggagttcct cgtcggcgtc gaagggctgg cgctgttccg acagctcgtc
     2281 gagggcgacg acgccgatgc ccaggcccgg atcgacgagg tccgccgcat tgtcggcacg
     2341 gacgaggagg ccgtgtacgg gctcgggatg gacatccccg agctcaatgc gcgcgccggg
     2401 tacgcgaagt ggtcggagac ctacgacgag cccggcaatc ccctcatcac ggtcgaggag
     2461 ccggtagtcc aggagctggt cggcgcggcc ccgcccggcc tggcgctcga tgccgcctgc
     2521 gggaccgggc gccactcccg cttcctcgcc gagaacggcc acgacgtcat cggcgtcgac
     2581 ggctcacccg agatgctggc ccgcgccgcc gtgcacgtcg gcagcgggac gttcgtccag
     2641 ggcgacctcg aagcgctccc gctccgctcg gcgggcatcg acctggccgt gtgcgccctc
     2701 gccctggggc acctcgtcga gctggccccg gcggtcgccg agctcgcgcg ggtcgtgcgg
     2761 ccgggcgggc ggatcgtgct cagcgacctc catccgacca tgtcggcaac gggtggccag
     2821 gccctgttcc gcgccgccga cgggtccagc gccttcgtcc gcgagcaccc gcacgtccac
     2881 agcgagtacc tggcggcgtt cgccgccgcc gacctcgagg tgcgcgcctg cgtcgagccc
     2941 cggttcgacg agaccgccgc ggtcatgcag ggaccggccc acgacttcgt gccggaagca
     3001 acggtcgcgg cgttccgcgg cctgcccggc gcgctcgtct gggacctggc ccggcgatga
     3061 gcgtcgacct cgacca
//
LOCUS       DASPWO010000343         3984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1151262, whole genome shotgun sequence.
ACCESSION   DASPWO010000343 DASPWO010000000
VERSION     DASPWO010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151262"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="VEO00_07835"
     CDS             <1..1106
                     /locus_tag="VEO00_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthetase"
                     /protein_id="HYU57943.1"
                     /translation="GYRERMELAAVALVRGVPYVGADFRLDPPPAAGPLPVPAFAVIG
                     TGKRTGKTAIGGTAARVAAARGMDPVVVAMGRGGPEAPEVAHAPIDLQTLRDLVRAGR
                     HAASDYLEDALTANVTTVGARRIGGGLAGAPFATNAAQAADVAVSLGAGIVVLEGSGA
                     ALPPVAWDGAALVAPASLDPEYLRGYLGPYRILRSDLFVLTMGSGPDVGPEALSALES
                     HIRRLRPDAWFVVTDFVPTPLGDVEGRRVFLATTASADGARAQAAHLQKEHGARVVAT
                     SARLADRAALRADVEAAGPFDVLATELKAAAVEVAAELALERGAGVVFVDNRAVTLSG
                     DGELEDLLGRALELAARRAAERTARRAWGRPGE"
     gene            1106..2089
                     /locus_tag="VEO00_07840"
     CDS             1106..2089
                     /locus_tag="VEO00_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYU57944.1"
                     /translation="MRIRKRAQRHIVITDRQPGLPFSKGLTASQLMATGLSPYRSYQV
                     AERVEERLLDRGSTEVTSGEVRDLTVSVLEEVAGDRYAKNFVRWQEVAALDVPLVILI
                     GGATGVGKSTVATQLAARLGIVRVVATDAIREVMRAMLTRELMPTLHTSSFDADTALR
                     EPPPRPADKVIVGFREQTMAVAVGVHALIERAAMEGTPLIVEGAHIVPGFVDTGPWAD
                     RVLPVPLIVSVEDEDLHRNHFLARAAEATHRPYERYLTAFPNIRKVQKYIKSQALSHG
                     VPVVPSYNLDQTLAAVVDLVVEHATERLAGRRRGAAAGEREARQPVEGRIG"
     gene            2086..2748
                     /gene="fsa"
                     /locus_tag="VEO00_07845"
     CDS             2086..2748
                     /gene="fsa"
                     /locus_tag="VEO00_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HYU57945.1"
                     /translation="MRLFLDTANFDEIREINRWGVLRGVTTNPSLLAKEHGDVEKAWR
                     LILDEVDGDVSLETTEPEAEAMYAQGVHLAAMAENAVVKVPMTPDGLMAGKRLADEGV
                     RINVTLVFSPAQAILAAEIGAYIVSPFIGRIDDTASDGLYALRQICEIYDVQGYETEV
                     LAASLRHPMHVVEAALAGADIATAPYDVFTKLVRHPLTDKGNAQFLADWAALQKELQE
                     GA"
     gene            2750..>3984
                     /gene="rho"
                     /locus_tag="VEO00_07850"
     CDS             2750..>3984
                     /gene="rho"
                     /locus_tag="VEO00_07850"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HYU57946.1"
                     /translation="MASTIDRPSLESKVLGELQEIASGLGIEGTQRLRKGDLIDRIVA
                     AGGGDVSGDGAAAASGNGSTSPATAPSAGSPTAPSRGADGTTARAAEGRSERPGGGVG
                     TQERPREDRRDRDRDRFDDRGGRRRRDRRRGGGGGGGGGRPIEREATEEELAEAPQIT
                     GILDIHNDGYGFLRTGGYLPGNRDVYVSLSQARRFGLRKGDEVTGLIRSPKDGEKYPA
                     LLKIETINELDPEAIRERRDFDKLTPLFPHERVRLEHDPTSVAERIIDLIAPIGKGQR
                     GMVVSPPKAGKTTVLKQIANGFIHADPKLHMIILLVDERPEEVTDWQRTVEAAEVVYS
                     TFDKPPDQHIQVTELVLERAKRLVESGRDVAILLDSITRLSRAYNLAAPASGKILSGG
                     IDSTALYPPRRFFGAARNL"
BASE COUNT          592 a         1396 c         1486 g          510 t
ORIGIN      
        1 tcggctaccg cgagcggatg gagctcgccg ccgtggccct ggtgcgcggg gtgccctacg
       61 tcggagcgga cttccgcctg gatccaccgc cggcggcggg cccgctcccg gtcccggcgt
      121 tcgccgtgat cggcaccggg aagcgaacgg gcaagacggc gatcggcgga accgccgcgc
      181 gggtcgccgc ggcgcgagga atggatccgg tggtggtggc catggggcgc ggcggcccgg
      241 aggcgcccga ggtggcgcac gcccccatcg acctgcagac gctgcgcgac ctcgtccggg
      301 cggggcgcca cgcggcgagc gattacctcg aggacgcgct gacggccaac gtcacgacgg
      361 tcggggcgcg gcggatcggc ggggggctcg ccggcgcgcc gttcgcgacg aacgcggccc
      421 aggcggcgga cgtggccgtg tccctcggcg ctgggatcgt ggtgctcgag gggagcggcg
      481 cggcgctccc gccggtggcc tgggacggcg cggccctcgt cgcgcccgcc tcgctcgacc
      541 ccgagtacct gcgcggctac ctcggcccct accggatatt gcgatcggac ctgttcgtcc
      601 ttacaatggg ttctggcccg gacgtcgggc cggaagccct ttcagcgctc gaatcccaca
      661 tccgccggct gcgtccggac gcgtggttcg tcgttaccga cttcgtgccg acgccgctcg
      721 gggacgtgga ggggagacgg gtgtttctcg caacgaccgc ctctgccgac ggtgcacgcg
      781 cccaggccgc acatctccag aaggagcacg gcgcccgcgt ggtggcgacg agcgcccgcc
      841 tcgccgaccg cgccgccctg cgcgcggacg tcgaggccgc cggcccgttc gacgtgctgg
      901 ccaccgagct gaaggcggcc gccgtggagg tggccgcgga gctggccctg gagcgcgggg
      961 cgggagtggt gttcgtggac aaccgcgcgg tgaccctctc cggcgacggg gagctggagg
     1021 acctcctcgg gagggccctc gagctcgccg cccgccgggc ggcggagcgc accgcgcgcc
     1081 gagcgtgggg gcgaccgggg gagtgatgag gatccgcaaa cgcgcccagc gacacatcgt
     1141 gatcaccgat cgccagccgg gcctcccctt ctcgaagggc ctgacggcct cgcagctgat
     1201 ggcgaccggg ctgtcgccgt accgctcgta ccaggtcgcc gagcgggtcg aggagcgcct
     1261 gctcgaccgc ggctcgaccg aggtgacgag cggcgaggtg cgcgacctga ccgtgtccgt
     1321 gctcgaggag gtcgccggcg atcgctacgc caagaacttc gtccggtggc aggaggtggc
     1381 ggcgctcgac gtgccgctcg tcatcctgat cggcggggcc accggcgtcg gcaagtcgac
     1441 ggtggccacc cagctcgcgg cgcgcctggg gatcgtgcgc gtggtggcca ccgacgccat
     1501 ccgcgaggtc atgcgggcga tgctcacgcg tgagctgatg ccgaccctgc acacgtcctc
     1561 gttcgacgcc gacaccgccc tgcgcgagcc cccaccccgc ccggccgaca aggtgatcgt
     1621 cggcttccgc gagcagacga tggcggttgc ggtcggtgtg cacgcgctga tcgagcgcgc
     1681 cgcgatggag gggacgcccc tgatcgtgga gggggcgcac atcgtccccg ggttcgtcga
     1741 caccggcccg tgggccgacc gggtgctccc ggtgccgttg atcgtctcgg tcgaggacga
     1801 ggacctacac cgcaaccact tcctggcccg agcggccgaa gccacccacc ggccctacga
     1861 gcgctacctg accgcgtttc ccaacatccg gaaggtgcag aagtacatca agagccaggc
     1921 cctctcgcac ggcgtgccgg tggtgccctc ctacaacctg gaccagacgc tcgccgcggt
     1981 ggtggacctg gtggtcgagc acgccaccga gcggctggcc gggcggcgtc gcggggcggc
     2041 cgcgggcgaa cgggaggcgc ggcagccggt cgaggggagg atcggatgag gctgttcctg
     2101 gacaccgcga acttcgacga gatccgcgag atcaaccgct ggggggtcct gcgcggggtg
     2161 accaccaacc ccagcctgct ggcgaaggag catggtgacg tcgagaaggc ctggcggctg
     2221 atcctcgacg aggtggacgg cgacgtgtcg ctcgagacca cagagcccga ggccgaggcg
     2281 atgtacgcgc agggcgtgca cctcgccgcg atggcggaga acgccgtggt caaggttccg
     2341 atgaccccgg acggcctgat ggccgggaag cgcctcgccg acgagggcgt ccggatcaac
     2401 gtgacgctcg tcttctcccc ggcgcaggcg atcctggccg ccgagatcgg cgcctacatc
     2461 gtctcgccgt tcatcggccg gatcgatgac acggcgtccg acgggttgta cgctttgcgg
     2521 cagatctgcg agatctacga cgtccagggc tacgagaccg aggtcctggc agcctccctc
     2581 cgtcatccca tgcacgtcgt ggaagccgcg ctcgccggcg ccgacatcgc caccgcgccg
     2641 tacgacgtgt tcacgaagct cgtgaggcac ccactcaccg acaagggcaa cgcccagttc
     2701 ctcgcggact gggcggccct ccagaaggaa ctgcaggaag gagcttagat tggccagcac
     2761 gatcgatcga ccgagcctcg aatcgaaggt cctcggcgaa ctccaggaga tcgcctcggg
     2821 cctcgggatc gagggaaccc agcgcctgcg caagggcgac ctgatcgacc gcatcgtcgc
     2881 cgcgggcggc ggcgatgtca gcggcgacgg ggccgcagcg gcgtccggga acggatccac
     2941 atccccggcc accgctccct cggccggcag tccgacagca ccctcgcgcg gggccgacgg
     3001 aaccaccgcg cgggccgcgg agggccggtc ggagcggccg ggcggcggcg tcgggacgca
     3061 ggagcgtccg cgcgaggatc gccgcgaccg tgaccgggac cggttcgacg accgcggcgg
     3121 acggcgacgg cgcgaccgtc gccggggcgg cggcggagga ggcggcggcg gccggcccat
     3181 cgagcgcgag gccaccgagg aggagctggc cgaggcgccg cagatcaccg gcatcctcga
     3241 catccacaac gacggctacg gcttcctgcg caccggcggg tacctgccgg gcaaccggga
     3301 cgtctacgtc tcgctctcgc aggcgcggcg gttcggcctg cgcaagggcg acgaggtcac
     3361 cgggctgatc cgctcgccga aggacggcga gaagtacccg gccctgctga agatcgagac
     3421 gatcaacgag ctcgaccccg aggcgatccg ggagcggcgg gatttcgaca agctgacgcc
     3481 gctgttcccg cacgagcggg tccgcctgga gcacgacccg acgagcgtgg ccgagcggat
     3541 catcgacctg atcgcgccga tcgggaaggg ccagcgcggg atggtcgtct cgccgccgaa
     3601 ggcagggaag acgaccgtgc tgaagcagat cgcgaacggc ttcatccacg ccgacccgaa
     3661 gctgcacatg atcatcctgt tggtcgatga gcgcccggag gaggtcaccg actggcagcg
     3721 cacggtggag gctgcggagg tcgtgtactc gaccttcgac aagccgccgg accagcacat
     3781 ccaggtgacc gagctggtgc tcgagcgcgc caagcgcctg gtcgagagcg gacgcgacgt
     3841 ggcgatcctg ctcgactcga tcacccggct gtcgcgggcc tacaacctgg ccgcgccggc
     3901 gtcggggaag atcctgtcgg gcggcatcga ctccacggcc ctgtacccgc cgcggcggtt
     3961 cttcggggcg gcccggaacc tcga
//
LOCUS       DASPWO010000344         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_472336, whole genome shotgun sequence.
ACCESSION   DASPWO010000344 DASPWO010000000
VERSION     DASPWO010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472336"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VEO00_07855"
     CDS             complement(<1..895)
                     /locus_tag="VEO00_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYU57947.1"
                     /translation="MDDGRSRMLNPAVARVARWGIVAWSAIGVVILAYFAYRYLLRPI
                     RVVFPPLVLALVLVYLLNPIVSRLQRRGIPRGWATLLTYVVFLTIVGLGMRFLVPLLA
                     EQVSGFIRGLPGLLDRVQVWISDLAERFNLNIDARQIVSDLGPGGGGGEFIGRIFSFT
                     AGILHVAVIVILGPILAFYLLVDLPKIQRGFDALIPMKRRDEVRSILDRMSRALGGFF
                     RGQLLVALFVGLASMFGLWVVGLPYWALVGMIAGLFNLIPLVGPFIGAVPALFIAFTT
                     VQSEGLLHLDPGWPLALGASAA"
     gene            1051..1479
                     /locus_tag="VEO00_07860"
     CDS             1051..1479
                     /locus_tag="VEO00_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYU57948.1"
                     /translation="MRVLVIDDEPDVLLLCRVNLEFEGFDVLEASDGERGIDIARGEH
                     PDVIILDVMMPRMDGLTVLDHLSHDPSTSDIPVIMLTAKAQEKDQIRGWVAGASDYIT
                     KPFSPGVLIDAVAKVASMSREELRARRAETLSRLSFLNRL"
     gene            1536..2603
                     /locus_tag="VEO00_07865"
     CDS             1536..2603
                     /locus_tag="VEO00_07865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU57949.1"
                     /translation="MLPEVPVHDRRLDEYREAAGDEAVERLRDVAEPLKGLRLLHLNS
                     TSFGGGVAELLFTQVGLLRDLGIDAHWHLIEGAEDFFTVTKAAHNGLQGAEVAWTETM
                     EEIYLERLRANARALTDTWDVTFVHDPQPAAIRTILAEEGETDGTWVWRCHIDLSAPF
                     EPVWEFLARWVESYDTAVFTSEEFVRPGFSGPRIAIVPPSIDPLSPKNAWLDHEVVWD
                     VVRRYGVDTQRPIALQVSRFDPWKDPIGVIEAYRIAKRSVPDLQLAMIGSIAADDPEG
                     WHYLQVTQHGRDCDPNVHLLTNLQEVGNLEVNAFQRAATVVLQKSLREGFGLTVSEAC
                     GRRRRSSAATSPGSGCRSRKA"
     gene            2600..2776
                     /locus_tag="VEO00_07870"
     CDS             2600..2776
                     /locus_tag="VEO00_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57950.1"
                     /translation="MTGFLVGSVEEAAARLTELIADRWLRAALGRAGRDHVRRRFLTL
                     RELEDYLRLVASLR"
     gene            2808..>3308
                     /locus_tag="VEO00_07875"
     CDS             2808..>3308
                     /locus_tag="VEO00_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HYU57951.1"
                     /translation="MASNRGPVAFEEVDGELVPRRGSGGLVTGLTSALQETGGRWVAA
                     ALTEGDRARAAEGAFTAEAEGAAYDLRYLAIEPERFENHYNGFANRVLWFCHHYLWDL
                     WRTPSFGPETERWWAAYRAVNAEFADALAAEAAAAGGAPAVLVQDYHLCLLPALLRER
                     ARRVRVA"
BASE COUNT          532 a         1137 c         1193 g          446 t
ORIGIN      
        1 gcgcggcgga cgccccgagc gccagcggcc acccgggatc caggtgcagc agcccctcgc
       61 tctgcaccgt ggtgaaggcg atgaacaggg cgggcacggc gccgatgaac ggtccgacca
      121 gcgggatcag gttgaacagc ccggcgatca tcccgacgag cgcccagtac ggcaggccga
      181 cgacccacag cccgaacatc gaggcgagcc cgacgaacag cgccaccagg agctggccgc
      241 ggaagaatcc gccaagcgcg cggctcatcc ggtcgaggat cgagcggacc tcgtcgcggc
      301 gcttcatcgg gatcagcgcg tcgaagccac gctggatctt cggcaggtcg accagcaggt
      361 agaacgccag gatcgggccg aggatgacga tcaccgcaac gtggaggatg ccggcggtga
      421 agctgaagat ccggccgatg aactcgcccc cgccgccccc ggggccgagg tcgctgacga
      481 tctggcgggc gtcgatgttc aggttgaagc gctcggcgag atcggagatc cacacctgca
      541 cccggtcgag gaggcccgga agtcccctga tgaaccccga cacctgctcg gcgagcagcg
      601 ggaccaggaa gcgcatgccc aggccgacga tcgtcaggaa caccacgtag gtcagcaggg
      661 tcgcccaccc gcgcgggatg ccgcgtctct gcaggcgcga gacgatcggg ttcaggaggt
      721 agaccagcac cagggcgagc acgagcggcg ggaagaccac ccggatcggc cgcaagaggt
      781 agcggtacgc gaagtacgcc aggatgacga cgccgatcgc cgaccacgcg acgatgcccc
      841 agcgggcgac ccgcgccacc gcggggttga gcatccgaga ccggccgtcg tccaccgcgg
      901 tcgattctag gtcaggcccg gggcccggcg gtcagtggtc gggggtgatt ggcccgaacg
      961 gactaggttg aaatgggccg gatgggggat catccggtgg tcgccgggcg ccgatggatg
     1021 agacgagcgg ccagaggagg aagcctgccg atgcgcgtgc tggtgatcga cgacgagccc
     1081 gacgtcctgc tcctgtgtcg cgtcaacctg gagttcgagg ggttcgacgt gctcgaggcg
     1141 agcgacggcg agcgcgggat cgacatcgcc cggggcgagc acccggacgt gatcatcctc
     1201 gacgtgatga tgccgcgcat ggacggcctg accgtcctcg accatctgtc ccacgacccg
     1261 tcgacgtcgg acatcccggt gatcatgctc accgcgaagg cgcaggagaa ggaccagatc
     1321 cgcggctggg tggccggcgc ctccgactac atcacgaagc cgttctcgcc gggcgtgctg
     1381 atcgacgcgg tcgccaaggt cgcctcgatg agccgtgagg agctccgcgc ccgccgcgcc
     1441 gagacgctct cccgcctctc gttcctgaac cggctgtagg tcctccaagc ccccccgcgg
     1501 tctggccgtg tccgcgcctc ggtagactgg cgaccatgct cccggaggtc cccgtccacg
     1561 accggcgcct cgacgagtac cgggaggccg cgggggacga agccgtcgag cggctgcgcg
     1621 atgtcgccga gccgctgaag ggcctgcggt tgctgcatct gaacagcacg tcgttcggcg
     1681 ggggcgtcgc cgagctgctg ttcacccagg tcggactgct gcgcgacctg gggatcgacg
     1741 cccactggca cctgatcgag ggggcggagg acttcttcac ggtgacgaag gccgcgcaca
     1801 acggcctgca gggggccgag gtcgcctgga ccgagaccat ggaggagatc tacctcgagc
     1861 gcctccgggc gaacgcgcgg gcgctgacgg acacctggga cgtcacgttc gtccacgacc
     1921 cgcagccggc cgcgatccgg accatcctcg ccgaggaagg cgagaccgac gggacctggg
     1981 tctggcggtg ccacatcgac ctgtcggcgc cgttcgaacc ggtttgggag ttcctcgccc
     2041 gatgggtcga gtcctacgac accgccgtgt tcaccagcga agagttcgtc cgaccggggt
     2101 tctccggtcc gcggatcgcc atcgtcccgc cgtcgatcga cccgctgtcc ccgaagaacg
     2161 cctggctcga ccacgaggtg gtctgggacg tcgtgcgccg ctacggggtg gacacgcagc
     2221 gcccgatcgc gctgcaagtg tcccgcttcg atccctggaa ggatccgatc ggcgtgatcg
     2281 aggcgtaccg catcgccaaa cgaagcgtcc cggacctgca actggcgatg atcggatcga
     2341 tcgccgccga cgaccccgag ggatggcact acctgcaggt cacccagcac ggtcgcgact
     2401 gcgacccgaa cgtccatctc ctgaccaacc tgcaggaggt gggcaacctg gaggtgaacg
     2461 cgttccagcg cgccgcgacc gtcgtcctgc agaagtcgct gcgcgagggg ttcgggctga
     2521 ccgtgtccga ggcgtgtgga aggagaaggc gttcatcggc ggcaacgtcg ccgggatccg
     2581 gctgcaggtc gaggaaggct tgacaggctt cctggtcggc tcggtggagg aggccgccgc
     2641 ccgactgacc gagctgatcg ccgaccggtg gctgcgcgcc gcgctcggcc gcgcggggcg
     2701 cgaccacgtc cgccggcgct tcctgaccct ccgcgagctc gaggactacc tgcgcctggt
     2761 cgcctcactc cgctgacgtg gcccccgccc gggaacgtcg gctgttcgtg gcatcgaacc
     2821 gcggaccggt ggcgttcgag gaggtggacg gggagctcgt cccgcgccga gggtccgggg
     2881 ggctggtcac cgggctcacc tcagccctgc aggagaccgg cgggcgctgg gtcgccgccg
     2941 cgctgaccga gggcgaccgg gcccgagccg cggagggcgc cttcaccgcc gaggccgagg
     3001 gcgccgcata cgacctgcgc tacctggcga tcgagccgga acggttcgag aaccactaca
     3061 acgggttcgc caaccgcgtg ctgtggttct gccaccacta tctgtgggac ctgtggcgca
     3121 ccccctcgtt cgggccggag accgagcggt ggtgggccgc ctaccgcgcg gtgaacgccg
     3181 agttcgcgga cgccctcgcg gcggaggcgg cggcggccgg cggcgccccg gcagtgctcg
     3241 tccaggatta ccacctctgt ctgctcccgg cgctcctccg cgagcgcgcg cggcgggtgc
     3301 gggtcgcc
//
LOCUS       DASPWO010000345         4219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_818675, whole genome shotgun sequence.
ACCESSION   DASPWO010000345 DASPWO010000000
VERSION     DASPWO010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818675"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VEO00_07880"
     CDS             <1..936
                     /locus_tag="VEO00_07880"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HYU57952.1"
                     /translation="LSAQGATGGAADGGGIFNGGGPLSVISSSITTGTVHAQSGGGNA
                     SAGCGGIHQTANAHLSLVRSSVDRNHVAAGAGPDDSNAGGGGMCASGSASIVGSTVSR
                     NTALATTADGIARARGGGLWLTSQSDDGAITNSTIANNTVTAKETGSGSIKSFGGGID
                     TFLSPLVLTDATVAANKVAGTGTQSFPMSGGGVFVDGSGTVTLAATILARDLAHGVGA
                     DCFGSVGSNGHNLVQKPSGFCTFAKKPSDQVNVDPKLGLLADNGGPTETMAIGATSPA
                     LDAILAAACAVAKDQRGVPRPQGPSCDIGAYERKI"
     gene            1042..3771
                     /locus_tag="VEO00_07885"
     CDS             1042..3771
                     /locus_tag="VEO00_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYU57953.1"
                     /translation="MVTGSRSNTKRTSLLTIAGLVTALLAWAAPGIAVSGAAAGGPAL
                     ALTPPSGPPGTEVVAEGSGFSPSGVVQISFDFAPVGETTAAGDGSFSANFTVPDSATP
                     IRHRVRAVEPASGLRASAVFTVTTAWPMLGFDAGRTGANPFENVLTPERVRGLSLDVL
                     TNDRATSSAVYARGGLYVGMADGSVRFLKTSTGLPTWSYLTRMPVRSTPALVFDARIR
                     SVVQDAVVVGSDDGRVYALAADTGEKLWRFQTGGPVRSSPLVLPDVRPTGAPSGLVVV
                     GSNDGNLYALSLANGSRVWAAPAGGPVVSSAAFAVWVTAGAAGHRPTAVEHPTVAVGS
                     TDGTLRAFDARTGDPLWSRDLGGKVVASPMVLPSSSLSSPGCDGIVGASTAGKVALLE
                     CSTGHRMWTVDVGSPVVGSPALFTCPPTPGAASCREVVVGTVGGELHGIDVPGGADSI
                     WIDFGHPIRSQPAIAGGIAWVHTSPTRTGAGTLQGWLLVAKQRAARIELPTEPPVANP
                     GSGTAFSPLVADAQVLLPTVKLFIKSDWPRFHQNLASTGFNAFEWKLGTNNVPGLHLL
                     WLGPTAGNTGTRSSPAVADGVAYIGAENGKVYAFPASCSNPCSPLWTGQTDDAVVSTP
                     VVASGVVYVGSRDTKLYAFPASCSNPCSPLWTGGTGTGFTNPAPIVSSPVVANGVVYV
                     GALGGNVFAFPASCSNPCSALWHRFVNGGVSSSPAVAAGRMFVGGLDGRLYAFPTNCS
                     NPCSPSWTSQAGEAIESSPAVANGVVYVGSRDGKLRAYPAACSSPCSPLWTATMSGEI
                     DDGPAVAKGVVYVGSTGSVSSAFSAFPASCSNPCSPLWSADIAVADSAAVANGVVYVG
                     STDEKLSAFPATCSNPCAPLRSIATGDIVDSSPAVADGVVYLGYQNRFAAFGT"
     gene            complement(3777..>4219)
                     /locus_tag="VEO00_07890"
     CDS             complement(3777..>4219)
                     /locus_tag="VEO00_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYU57954.1"
                     /translation="RRLPVRVVDRIALILQRLTDGEALEDQGDPILDVGLIGLLKKRR
                     VGVFPAVERLDGPDVVLDGGRRLRPDAVIWATGFQRGLERLVGHLGAASGRPAVHGAA
                     THPAAPGLHFIGFSNPISGNLRETAIDAKRIARAVRRRRQPAAS"
BASE COUNT          527 a         1528 c         1539 g          625 t
ORIGIN      
        1 ctctcggcgc agggcgccac cggcggggcg gccgacggtg gcgggatctt caacgggggc
       61 ggcccgctct ccgtgatctc gtcctcgatc acgacgggaa cggtccacgc ccagtcgggc
      121 ggggggaacg cgagcgccgg ctgtggcggg atccatcaga cggcgaacgc gcacctctcg
      181 ctcgtgaggt cctcggtgga ccgcaaccac gtcgcggccg gtgctggtcc ggacgactcc
      241 aacgctggcg gcggcggcat gtgcgcgtcg ggatccgcct cgatcgtggg ctcgaccgtg
      301 agccggaaca ccgccctcgc caccaccgcg gacggcatcg cccgggcccg cggcggcggt
      361 ctgtggctca ccagccagtc cgacgacggc gcgatcacga actcgacgat cgcgaacaac
      421 accgtcaccg cgaaggagac ggggtcgggt tcgatcaagt cgttcggcgg cgggatcgac
      481 acgttcctgt cgcccctcgt gctcaccgac gcgacggtgg cggcgaacaa ggtggccggg
      541 accgggacgc agagcttccc gatgtccggc ggcggcgtgt tcgtcgacgg ctccggcacc
      601 gtgacgctgg cggcgacgat cctcgcgcgc gacctcgcgc acggcgtggg cgccgactgc
      661 ttcggatcgg ttggctcgaa cggccacaac ctggtccaga agccctcggg gttctgcacc
      721 ttcgcgaaga agccgagcga ccaggtgaac gtcgacccga agctcgggct gctggcggac
      781 aacggcgggc ccaccgagac catggccatc ggcgcgacca gccccgcgct cgacgccatc
      841 ctcgcggccg cctgcgccgt ggccaaggac cagcgcggcg ttccccggcc ccaggggccg
      901 agctgcgaca tcggcgccta cgagcggaag atctgatccc agccgaccgg gcgaatgctt
      961 ggcttgcgcg agtggcgggg ccgtggtccc cttttccaag gcccgcgctc gcagggttcg
     1021 gccggagcgc aaggagcggc catggtcacc gggagccggt cgaacacgaa acggaccagc
     1081 ctcctgacga tcgccggact ggtcaccgcg ctcctcgcct gggcggctcc gggcatcgcc
     1141 gtctcgggtg ccgccgccgg aggtcctgcg ctcgccctga cgccgccctc ggggccgcca
     1201 ggcacggagg tggtcgccga ggggagtggg ttcagtccct cgggcgtcgt tcagatctcg
     1261 ttcgacttcg cgccggtggg ggagaccacc gccgcgggag acgggtcgtt ctctgcgaac
     1321 ttcacggtgc cggactccgc cacgccgatc cggcatcggg tgcgggcggt ggagccggcc
     1381 tccgggctgc gcgcctcggc ggtgttcacc gtgaccacgg cctggcccat gctcgggttc
     1441 gacgcggggc ggaccggcgc gaatcccttt gagaacgtgc tcacgcccga gcgggtgcga
     1501 gggctgtccc tcgatgtgct gacgaacgat cgggccacct cctctgccgt ctacgccagg
     1561 ggcggcctgt acgtggggat ggcggacggg agcgttcggt tcctcaagac gagcaccggg
     1621 ctccctacct ggtcttacct cacccgcatg ccggtgcggt cgacacccgc gctcgtgttc
     1681 gacgctcgga tccgctccgt cgtccaggac gccgtcgtcg tggggtcgga cgacgggagg
     1741 gtctacgcac tggcggccga caccggcgag aagctgtggc ggttccagac cggggggccg
     1801 gtccggtcgt cgccgctcgt gctgccggac gtccggccga ccggggcgcc ctcgggcctc
     1861 gtcgtggtgg ggtcgaacga cgggaacctg tacgccctct cgctggcgaa cggctcccgg
     1921 gtgtgggcgg ctcccgccgg cgggccggtc gtgtcgtcgg ccgcgttcgc cgtgtgggtg
     1981 acggccggcg cggccgggca caggcccacc gcggtcgaac acccgaccgt cgcggtcggg
     2041 tccaccgacg ggacgctgcg cgcgttcgac gcgcggaccg gcgatccgct gtggtcgcgc
     2101 gacctcgggg ggaaggtcgt cgcgtcgccg atggtcctcc cgagctcctc gctctcctcg
     2161 cccgggtgcg acgggatcgt cggggcgtcc accgccggca aggtcgccct gctcgagtgt
     2221 tcgaccggcc accggatgtg gacggtggac gtgggctcgc ccgtggtggg ctcgcccgcg
     2281 ctcttcacgt gtccgccgac tccgggggcg gcctcctgcc gcgaggtggt ggtcggcacc
     2341 gtcggcgggg agctccacgg catcgacgtg cccggcgggg cggattcgat ctggatcgac
     2401 ttcgggcacc cgatccggtc ccagcccgcg atcgcgggcg ggatcgcctg ggtgcacacc
     2461 tctccgacga ggaccggcgc cggcaccctc caggggtggc tcctcgtggc caagcagcga
     2521 gccgcgcgga tcgagcttcc gaccgagccg ccggtggcca accccggctc tggcaccgcc
     2581 ttctcgcccc tggtggccga cgcccaggtc ctgctcccga cggtcaagct gttcatcaag
     2641 agcgactggc cgcggttcca ccagaacctc gcgagcaccg gcttcaacgc gttcgagtgg
     2701 aagctgggaa cgaacaacgt ccccgggctg cacctgctgt ggctggggcc gacggcgggg
     2761 aacacgggca cccgctcctc gccggctgtc gcggacgggg tcgcctacat cggcgccgag
     2821 aacgggaagg tctacgcgtt ccccgcgtcc tgttcgaacc cgtgctcccc gctgtggacg
     2881 gggcagacgg acgacgccgt ggtgtcgacg cccgtcgtgg cgagcggcgt cgtctacgtc
     2941 ggctcccgcg acaccaagct gtacgccttc cccgcctcct gttcgaaccc gtgctcgccg
     3001 ctgtggaccg gcggcaccgg gacgggcttc acgaaccccg cgccgatcgt ctcctcgccg
     3061 gtcgtggcga acggcgtggt gtacgtcggc gcgctcgggg ggaacgtgtt cgccttcccc
     3121 gcctcctgtt cgaacccgtg ctcggcgctc tggcaccgat tcgtcaacgg cggcgtgtcg
     3181 tcgtcgcccg cagtggccgc cgggcggatg ttcgtcggcg ggctcgacgg gaggctgtac
     3241 gcgttcccga ccaactgctc gaacccgtgc tccccctcct ggacctcgca ggcaggcgaa
     3301 gcgatcgagt cctccccggc cgtggcgaac ggcgtggtct acgtgggatc gcgcgacggc
     3361 aagctccgcg cgtacccggc cgcgtgctcg agcccgtgct cgcccctgtg gaccgccacc
     3421 atgagcggcg agatcgacga cggtccggcc gtggccaagg gcgtggtcta cgtgggatcg
     3481 acggggtcgg tgtcgagcgc cttctccgcc ttcccggcgt cctgctcgaa cccgtgctcc
     3541 ccgctgtggt cggcggacat cgccgtcgcc gactccgccg cggtggcgaa cggtgtcgtc
     3601 tacgtcggct cgaccgacga gaagctctcc gcgttcccgg cgacctgttc gaacccgtgc
     3661 gccccgctcc ggagcatcgc gaccggggac attgtcgact cgtcgcccgc cgtggcggac
     3721 ggcgtggtct acctcggata ccagaaccgg ttcgccgcgt tcggtacgta gcccggtcag
     3781 ctcgcggcag gttggcgccg gcgacggacc gctcgggcga tgcgcttggc gtcgatggcg
     3841 gtctcgcgaa ggttgccgct gatcgggttc gagaagccga tgaagtgcag cccgggcgcg
     3901 gccgggtggg tcgctgctcc gtggactgcc gggcggccgc tcgcggcgcc caggtggccg
     3961 accagccgct ccaggccgcg ctggaacccc gtggcccaga tcacggcgtc ggggcggaga
     4021 cggcggccgc cgtccagcac gacgtcgggg ccgtcgaggc gttccacggc cgggaacacc
     4081 cccacccgcc gcttcttcag cagcccgatc aggccgacgt ccaggatcgg atcgccctga
     4141 tcctccagcg cctcaccgtc ggtgaggcgc tggaggatca gggcgatccg atcgaccacg
     4201 cggaccggca gcctgcgga
//
LOCUS       DASPWO010000346         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_818720, whole genome shotgun sequence.
ACCESSION   DASPWO010000346 DASPWO010000000
VERSION     DASPWO010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818720"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VEO00_07895"
     CDS             complement(<1..1092)
                     /locus_tag="VEO00_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU57955.1"
                     /translation="MDFQLSEDQITQRKWLHEFAEREIRPVAPQYDEAEEFPWPVLKK
                     AAEAGIYSLEFYTEIAAGDPTGLGLPIALEETCWGCAGIALSVFGTGLPLASLAYTAT
                     PDQLQEWAPKMFGTPQDPKLGAFAVTEPNAGSDVSALRTTAKRDGDEWVLNGQKVFIT
                     NGGIADVHVVVATVDPELGHRGQASFIVGPGTPGLKQGKKERKLGIRASHTAEVLLED
                     CRIPIENVVGGMEKLDAKLERARGAGGTRASTALKTFEMTRPSVAAQAVGIARAALEF
                     AVDYSKDRQTFGRPIIEHQGVAFKLADMAMEVDAARLLTWRAAWMARTGSDFPHAEGS
                     MSKLKAGEVAVRVTEEAVQILGGYGFIKDF"
     gene            1147..1761
                     /locus_tag="VEO00_07900"
     CDS             1147..1761
                     /locus_tag="VEO00_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYU57956.1"
                     /translation="MAARSQPATKRRMRGPERRAQLLQVARSVFGRRGFHSVSMDQVA
                     KEAGVTKPILYDHFPSKKELYLALLDADLAELHEKVRAALDGSPGNRERIRASFQAYF
                     DFVDDHAEGFRLLMTESIGAELDFRERVGRVRDDILEEVTELIARESGGGLTRDAART
                     VALGLIGMAETVAQQEPGRPKETRRASVDVLVRLAWKGITGLTL"
     gene            1771..>2020
                     /locus_tag="VEO00_07905"
     CDS             1771..>2020
                     /locus_tag="VEO00_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57957.1"
                     /translation="MDLDMGAALAEARRLFRPISWRGATPDPKDVDCLLFDRTRRVTS
                     FDGTEIAYDVYGRRGPWVALVPGFSCPDSFWRYMLPDLA"
BASE COUNT          268 a          738 c          697 g          317 t
ORIGIN      
        1 gaagtccttg atgaagccgt agccgccgag gatctggacg gcctcctcgg tgacgcgcac
       61 ggcgacctcg ccggccttca gcttgctcat cgagccctcg gcgtgcggga agtccgagcc
      121 ggtgcgcgcc atccacgccg cgcgccaggt gagcaggcgc gccgcatcca cctccatcgc
      181 catgtcggcg agcttgaacg ccacgccctg gtgctcgatg atcggtcgcc cgaacgtctg
      241 gcggtccttc gagtagtcca ccgcgaactc gagcgccgcc cgcgcgatcc cgacggcctg
      301 cgccgccacc gacggccgcg tcatctcgaa cgtcttcagc gccgtggagg cgcgcgtccc
      361 gccggcgccg cgggcacgct cgagcttcgc atcgagcttc tccatcccgc cgaccacgtt
      421 ctcgatcggg atgcggcagt cctcgagcag gacctcggcg gtgtgggatg cgcggatgcc
      481 gagcttcctc tccttcttcc cctgcttcag ccccggcgtg ccgggcccga cgatgaacga
      541 cgcctggccg cggtggccga gctcggggtc gaccgtcgcc accaccacgt ggacgtcggc
      601 gatgccgccg ttcgtgatga agaccttctg gccgttcagc acccactcgt cgccgtcgcg
      661 cttcgcggtg gtgcggagcg ccgacacgtc ggagcccgcg ttcggctcgg tgaccgcgaa
      721 cgcgccgagc ttcgggtcct ggggcgtccc gaacatcttc ggcgcccact cctgcagctg
      781 gtcgggggtg gccgtgtagg cgagcgaggc gagcggcagg ccggtcccga agaccgagag
      841 ggctatgccg gcgcagcccc agcaggtctc ctccagcgcg atcggcagcc ccagcccggt
      901 cgggtcgccg gcggcgatct ccgtgtagaa ctccagcgag tagatcccgg cctcggcggc
      961 cttcttcagc accggccagg ggaactcctc ggcctcgtcg tactgcggcg cgaccggccg
     1021 gatctcccgc tccgcgaact cgtggagcca cttgcgctgg gtgatctggt cctcgctgag
     1081 ctggaagtcc atcggctctc cctaagttac ctgtcggtag gttacctcac ggtatagtac
     1141 cccgtcatgg cggcacggag tcaaccggcg accaagcgcc ggatgcgcgg tcccgagcgg
     1201 cgggcccagt tgctgcaggt cgcccggtcg gtgttcggcc gccgcggctt ccactcggtc
     1261 tcgatggatc aggtggcgaa ggaggccggc gtcaccaagc cgatcctcta tgaccacttc
     1321 ccgtcgaaga aggagctcta cctggcgctg ctcgacgccg acctggccga gctgcacgag
     1381 aaggtgcgcg cggctctcga cggatcaccg gggaaccgcg agcggatccg ggcctccttc
     1441 caggcatact tcgacttcgt cgacgaccac gcggaggggt tccgcctgct gatgaccgag
     1501 tcgatcgggg ccgagctgga cttccgcgag cgggtgggcc gcgtccgcga cgacatcctg
     1561 gaggaggtca ccgagctgat cgcgcgcgag tccggcggcg ggctcacccg cgacgcggcc
     1621 cgcacggtgg ccctcgggct gatcgggatg gccgagacgg tggcgcagca ggagccgggc
     1681 cgcccgaagg agacccggcg cgcgtcggtg gacgtcctgg tccggctggc ctggaagggc
     1741 atcaccgggc tgacgctgta ggcctcgccc gtggacctcg acatgggcgc ggcgctcgcc
     1801 gaggcccggc gcctgttccg cccgatctcc tggcgcggcg ccacgccgga cccgaaggac
     1861 gtcgactgcc tgctcttcga ccgcacccgc cgcgtcacct cgttcgacgg caccgagatc
     1921 gcctacgacg tctacggccg gcgcggcccg tgggtggcgc tcgttcccgg attctcgtgc
     1981 cccgactcgt tctggcggta catgctgccc gacctggccg
//
LOCUS       DASPWO010000347         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1072552, whole genome shotgun sequence.
ACCESSION   DASPWO010000347 DASPWO010000000
VERSION     DASPWO010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072552"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VEO00_07910"
     CDS             <1..586
                     /locus_tag="VEO00_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU57958.1"
                     /translation="LSADKPEQEARALAQILDRILELPAAVVFVDEVEDLASMRHDQR
                     KVSPRVTNEFLKQIPRFRQASHHLLACATNSIGALDPAFLRPGRFDYILPVGPPDAKA
                     REAIWGRYVGEITDQQVDIDGLVAASEFFTPADIEFAAHKAAQRAFEQEHFEGSRERA
                     TTEDFLAAIRETKPTLSEEVIASFGKDVERFSRY"
     gene            1148..2035
                     /locus_tag="VEO00_07915"
     CDS             1148..2035
                     /locus_tag="VEO00_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HYU57959.1"
                     /translation="MSADLPMSVWATAQTHARQQRANRYVRESANHPGRMLPAIARAA
                     ISAYTSPGDLVLDPVCGVGTTLVEAIHLGRHAVGVEYEPVWADIARANLEFAAAQGAI
                     GRGEVITGDALRPSELVDREICGTVGMVLTSPPCGASVHGQATPGPGRGVIRSHHRYS
                     KDRTNLAHQPLGSLLSAFASILAACRDLLRSGGVVAVTARRSPAAGDRPRGRACVPSP
                     GGGVTRERPPPSGPGPDGSGARDPTSEGGRADSLQLRIRVRYAGGEAGRVLAVAQGRA
                     LREALEVLREVEVGQAEEQ"
     gene            2041..>2392
                     /locus_tag="VEO00_07920"
     CDS             2041..>2392
                     /locus_tag="VEO00_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HYU57960.1"
                     /translation="MTPTSARTAPRYAWYGRVSTEDEQDPTLSFPRQLANTESRVTKA
                     GGRIVAHYYDIESGTGALAGRGKGGLAGFAIPIRPDGGIHDLLTAASQRPRPFDRVIV
                     ESISRLSRNSSVAFR"
BASE COUNT          375 a          826 c          822 g          369 t
ORIGIN      
        1 gctgtcggcc gacaagcccg aacaggaggc cagggccttg gcgcagatcc tcgatcggat
       61 cctggagctg ccggcggccg tggtgttcgt agacgaggtc gaggacctgg cttcgatgcg
      121 ccacgaccag cggaaggtga gtccgcgggt cacgaacgag tttctgaagc agatccccag
      181 attccggcag gcgtcgcacc atctcctggc gtgtgcgacg aattcgatcg gcgcgctcga
      241 cccggcgttc ctgaggcccg gacgattcga ttacatcctc ccggtcggcc ctcccgacgc
      301 caaggcccgc gaggcgatct ggggacgcta cgtgggggag atcacggacc agcaggtcga
      361 catcgacggg ctcgtcgcgg ccagcgagtt cttcaccccg gcggacatcg agttcgcggc
      421 gcacaaggcg gcgcagcgag ccttcgagca ggagcatttc gagggaagcc gagagcgtgc
      481 caccaccgag gactttctgg ccgcgatcag ggagaccaag cccacgcttt cagaggaggt
      541 catcgcctca ttcgggaagg acgtcgagag attctccagg tattagctgt ggtttctgag
      601 ggcacggctg ggcggaacca gggctcgtga gcgcggcggc gctacgaacc gcagcgctgt
      661 tcatcacctc ggacttcgat cttcgccttg ccgctcagcc aacagataca ggaggttgct
      721 cccgctcagg agtctccagg ctcgcccgcc ccggacgagg gcgcgtccaa tgcggtgttg
      781 cggtcggcaa tgacggcgcc gctcacgacc atcagggtac ggcgaggctc ggacatccga
      841 tcggcacggc tcgcttctcg gaccggcggg gggactcagg cgcttggccg gtgtagcggt
      901 cagcaagccc gggcaggagg ctgaccatcc gcatccaccg caatctggcc cactcccggc
      961 ccgtattacg tggggtcacc tccagcacgt cgtcgcacta ctggccgcga tccgtgacgg
     1021 gggtctgttc gcccgggccc tcgttctggc agctcaccca gatccgtagg gcccgcgggc
     1081 ggggcgagcc ggcccacctc gtcgtgcacg aggacgtttg cgtcttcgct aagccggagg
     1141 agcgtcgatg agtgcggacc tcccgatgtc cgtgtgggcg acggcccaga cgcacgctcg
     1201 ccagcaacgc gccaaccgtt acgttcggga gtccgcgaac caccccggaa ggatgctccc
     1261 tgcgatcgcc cgtgcggcga tcagcgcgta cacctctccg ggcgacctcg tcctcgatcc
     1321 cgtgtgcggc gtggggacga cgctcgtcga ggccatccat ctcggtcgcc acgccgtggg
     1381 cgtggagtac gagcccgtat gggccgacat cgcccgggcg aacctcgagt tcgccgcggc
     1441 gcagggcgca atcgggcggg gcgaagtcat tactggggac gcccttcgcc cgtcggagct
     1501 ggtcgaccgc gagatctgcg ggacagtcgg catggtcctc acctctccac cctgcggggc
     1561 gtcggtgcac gggcaggcca cgcccggtcc gggccgcggc gtcatcaggt cacaccaccg
     1621 gtattcgaag gaccggacga acctggccca tcagcctctg ggcagcctgc tctccgcgtt
     1681 cgcctcgatc ctcgcggcgt gcagagatct gctccgctcg ggcggcgtcg tggccgtgac
     1741 ggcgcgccgg tcgcccgctg caggtgatcg cccacgagga cgtgcttgtg ttccgtcgcc
     1801 cggcggcggc gtgacccgag aacggccacc accgagcggg ccagggcccg acggctcagg
     1861 cgctcgggac ccgacctcag agggaggccg agccgattcg ctgcaactcc ggatccgtgt
     1921 ccgttacgcc ggaggggagg ccggccgagt gctggccgtc gcgcagggaa gggcgctcag
     1981 ggaagcgctt gaggttctcc gggaggtcga ggtaggacag gctgaggagc aatgacagcc
     2041 atgacgccaa cgtcggcccg aaccgccccc cgctatgcgt ggtacggccg ggtctccacg
     2101 gaggacgagc aggacccaac cctctcgttt ccgcgccagc tcgctaacac cgagtcccgc
     2161 gtgaccaagg cgggcggacg tatcgtcgcc cactactacg acatcgagtc cggcacaggc
     2221 gcgctggcgg gacgcggcaa aggcggcctg gcgggcttcg ccatcccaat ccggcccgac
     2281 ggcggcatcc acgacctcct caccgccgcg tcgcagcgac ctcgtccttt cgatcgcgtc
     2341 atcgtggagt cgatctcccg gctctcgcgc aactcctccg tggcgttccg gg
//
LOCUS       DASPWO010000348         6071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1236883, whole genome shotgun sequence.
ACCESSION   DASPWO010000348 DASPWO010000000
VERSION     DASPWO010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236883"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1849
                     /locus_tag="VEO00_07925"
     CDS             <1..1849
                     /locus_tag="VEO00_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57961.1"
                     /translation="TFTKLGGATFNQATIFEIVVQSSGNRLFVATNRGLYRSLDAGQS
                     WKKVLAPGGADQLGDFVTHVVELPGTAHNGLLAAVGWRGGHPKNGLYVSNNDGDTWSK
                     VNPVGFAPQGNIGRMTLAVHPDTPGLIYAVVQDAVLFNTGGATVLNGVYKTTAGPSGP
                     WTLVATSQIYANDPNSALDPLKIGPGYQPGIQAWYNQYVEIDPLDANHVVVGLEEIYD
                     TDDGGATWDTIGRYWNYCISSSQPNCNLNPNTHPTTHPDQHTAAFTEVNGVSKIFVGN
                     DGGVWSQTGPTLDNDHWLNRNTNLSTIQFYYTEPSGGPNYVVYGGTQDNGHIKYEGNP
                     TWPIVYGGDGGDAAVEPNNPNNAYEEYVYLTMAKTTDGGQTWTNIYTPDSGSSATARF
                     ISPFEMDPLSKNHIVALGRHVWETNIGISTQSDDWQNAYDLGAGHFGTALDLRGPTVY
                     AGWCGPCNPSPPVDPNGNGFARGLATNVGGTWHAVAANGLPNRYVTGIAIDPANANHV
                     FVTLSGFSRRWVPYAGNGSIFESTNGGQSFTSITKNLPDAPANDAILFNGRLIVATDV
                     GVYERAGDGTWGLLGTGLPKVSVLDLALVPGQGTLIAGSHGRGAWAIQL"
     gene            complement(1862..2755)
                     /locus_tag="VEO00_07930"
     CDS             complement(1862..2755)
                     /locus_tag="VEO00_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HYU57962.1"
                     /translation="MLERLDRFQQRHAWLAFPVAVAKKFGDDRAGYLAALIAYYGFFS
                     LFPLLLVLITVLSIVLQGDPDLRARILDTAVAQFPVIGDQIRQNVGEIRGSGLALAVG
                     IATALWAGLAVTAAVQNAMNEVWGVPRERRPSFVVRLARGLLLLAVLGTMTIAATLVS
                     SLAASVGDLSFLSRAVGLLGGLALQLALFLVVFKGMTRADVSVRDVLPGAAIAAVGWT
                     VLLAIGGWLVASRLRAASQIYGVFAFVIALLVWLYLGAQLTLYAAEVNVVRARRLWPR
                     SLFGRPSLVNGNERGRSPARP"
     gene            2917..3213
                     /locus_tag="VEO00_07935"
     CDS             2917..3213
                     /locus_tag="VEO00_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57963.1"
                     /translation="MRGILLGVVAAGILTVGAIGVVAQTPDATVIKESEIEQNEPHLV
                     DAANDPELQRLARDINETQRACAASAEAWANCPVDELRVMRAELEKQMERYAAK"
     gene            complement(3234..3953)
                     /locus_tag="VEO00_07940"
     CDS             complement(3234..3953)
                     /locus_tag="VEO00_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HYU57964.1"
                     /translation="MHVTGTLINAATVLVGTVLGTLFGNRLPDRIRETVMHSLGLVTL
                     VVGAGEAFEVFRAPLSGYRASLVVILGSVLAGGIVGELLRIEDRLESAGERLKRRFGQ
                     GQARFTEGFVVASLVFCVGPLTVLGSIEDGLRGDYDLLAIKALLDGFAALAFASALGW
                     GVGFSILTILVYQGSLSLGASALAGVFTPIVVAALTAAGGVLILGIGFRLLELKQVRV
                     ANLLPALVIAPVAMSLVERFA"
     gene            4017..4538
                     /locus_tag="VEO00_07945"
     CDS             4017..4538
                     /locus_tag="VEO00_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57965.1"
                     /translation="MSDRAIGHLDEVLDNLGVFHTHPQELLATCPSGFRRVPITQGDG
                     YYISFQGFGPDQPAFAHTHPDSEEWVIVLAGGGWALVDEHPVPLEPGSMVGRAMAHPH
                     GFVSGPEGLQLLSVQLPRPVEGATTWDAPGETTDPIDCAFGGRCRRCWRCGGHTRRMA
                     TGRFSCENCTLEF"
     gene            complement(4563..5078)
                     /locus_tag="VEO00_07950"
     CDS             complement(4563..5078)
                     /locus_tag="VEO00_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57966.1"
                     /translation="MERCWHCGTNPEDPGSARKVTMHSGSLERTVSVATCTACAAILH
                     RTELWLGGLVLLSVPAAIAAGLVAGNWLIGGGGFVLTCVLAGLASRSIRVRGGVRRAT
                     NTHPEVVDLKKAGWRLGRAPKAKATTGPAIGVLKTCSRCGRVVAPTAHRGESCPFCGT
                     YWSDEEWKRAG"
     gene            complement(5396..5743)
                     /locus_tag="VEO00_07955"
     CDS             complement(5396..5743)
                     /locus_tag="VEO00_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYU57967.1"
                     /translation="MSQERIEDAISTSRGLHGRIGDVQVLDVREADEWAAGHIEGSVH
                     LPLSRLMSGPADLDPERPVVAVCSMGSRSEVAMLMLRARGFVAYNLERGLQEWFAEGL
                     PLVSDDGRPPRVA"
     gene            complement(5869..>6071)
                     /locus_tag="VEO00_07960"
     CDS             complement(5869..>6071)
                     /locus_tag="VEO00_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYU57968.1"
                     /translation="PLKRYRDFLQTDSALFEASWPYLVNRGVFMTPGDEEQWTISVRH
                     DEDDIARYVDAFGEFCQEVVSS"
BASE COUNT         1033 a         2204 c         2064 g          770 t
ORIGIN      
        1 cacgttcacc aagctcgggg gcgccacgtt caaccaggcc acgatcttcg agatcgtcgt
       61 gcagtcatcc ggcaaccggc tgttcgtggc caccaaccgc ggcctgtacc gctccctcga
      121 cgccggtcag agctggaaga aggtcctggc ccccggcggc gccgaccagc tcggggactt
      181 cgtgacccac gtcgtcgaac tgcccggcac ggcccacaac ggcctgctgg cggcggtcgg
      241 ctggcggggg ggccacccga agaacgggct gtacgtctcg aacaacgacg gtgacacctg
      301 gagcaaggtc aacccggtgg ggttcgcgcc gcaggggaac atcgggcgca tgacgctcgc
      361 cgtgcacccg gacacgcccg gcctgatcta cgcggtggtc caggacgccg tgctgttcaa
      421 caccggcggc gccacggtcc tgaacggcgt gtacaagacg accgcggggc cgtccggccc
      481 gtggacgctc gtggcgacgt cgcagatcta cgcgaacgac ccgaacagcg cgctcgaccc
      541 cctgaagatc gggcccgggt accagcccgg catccaggcc tggtacaacc agtacgtcga
      601 gatcgatccg ctcgacgcga accacgtcgt ggtcgggctc gaggagatct acgacaccga
      661 cgacggcggg gcgacctggg acacgatcgg ccgctactgg aactactgca tctcctccag
      721 ccagccgaac tgcaacctga atcccaacac ccacccgaca acccaccccg accagcacac
      781 cgcggcgttc accgaggtga acggcgtgtc gaagatcttc gtcgggaacg acggcggcgt
      841 gtggtctcag accgggccga cgctcgacaa tgaccactgg ctgaaccgca acaccaacct
      901 gtcgacgatc cagttctact acacggagcc gagcggcggc ccgaactacg tcgtctacgg
      961 cggcacgcag gacaacggcc acatcaagta cgagggcaac ccgacgtggc cgatcgtcta
     1021 cggcggtgac ggcggcgacg ccgcggtcga gccgaacaac ccgaacaacg cgtacgagga
     1081 atacgtgtac ctgacgatgg ccaagaccac cgacggcggg cagacgtgga cgaacatcta
     1141 cacgcccgac tcggggagct cggccactgc gcggttcatc tcgccgttcg agatggaccc
     1201 gctgtcgaag aaccacatcg tggcgctggg gcgccacgtc tgggagacga acatcgggat
     1261 cagcacccag tccgacgact ggcagaacgc ctacgacctc ggggccggcc acttcggcac
     1321 cgcgctcgac ctgcgcgggc cgaccgtgta cgccggctgg tgcgggccgt gcaacccgag
     1381 cccaccggtg gacccgaacg ggaacggatt cgcccgcggc ctggccacga acgtcggcgg
     1441 gacctggcac gcggtggcgg cgaacgggct gcccaaccgc tacgtcaccg ggatcgccat
     1501 cgacccggcg aacgccaacc acgtgttcgt cacgctctcc ggcttctcgc gccggtgggt
     1561 gccgtacgcg gggaacggga gcatcttcga gtccacgaac ggcgggcagt ctttcaccag
     1621 catcacgaag aacctgcccg acgcgccggc caacgacgcc atcctgttca atgggcggct
     1681 gatcgtggcc accgacgtcg gcgtgtacga gcgcgccggc gacgggacgt gggggctgct
     1741 cggcaccggg ctgccgaagg tgagcgtcct ggacctggcg ctcgtgccgg gccagggcac
     1801 gctgatcgcc ggcagccacg gccggggcgc ctgggcgatc cagctgtaag cgccgcggga
     1861 gtcagggacg ggcggggctc cggccccgct cgttcccgtt cacgagcgag ggccggccga
     1921 agagcgaccg gggccacagc cggcgagcgc ggaccacgtt cacctcggcg gcgtacagcg
     1981 tcagctgcgc cccgaggtac agccagacga gcagggcgat gacgaaggcg aacacgccgt
     2041 agatctggct cgccgcacgg aggcgcgagg ccaccagcca tccgccgatg gcgagcagga
     2101 ccgtccagcc gaccgccgcg atcgccgcgc cgggaagcac gtcccggacg gagacgtcgg
     2161 cccgggtcat gcccttgaac acgaccagga agagggcgag ctgcagggcc agcccgccga
     2221 ggagaccgac cgctcgcgac aggaacgaca ggtcgcccac cgacgccgcc agcgaggaga
     2281 cgagcgtggc ggcgatcgtc atcgtcccga gcaccgcgag caggagcagg ccgcgcgcca
     2341 agcgcaccac gaacgacgga cgccgctccc gcgggacgcc ccacacctcg ttcatcgcgt
     2401 tctgcaccgc ggccgtcacc gcgagcccgg cccacagggc ggtggcgatc ccgaccgcga
     2461 gcgccagccc gctgccgcgg atctcgccga cgttctggcg gatctggtcg ccgatcaccg
     2521 ggaactgggc cacggcggtg tccaggatcc gggcccgcag gtcgggatcg ccctgcagga
     2581 cgatcgagag gaccgtgatc agcaccagga gcaacgggaa gagcgagaag aacccgtagt
     2641 acgcgatcag ggccgccagg tagccggcgc ggtcgtcgcc gaacttcttc gccaccgcga
     2701 cggggaacgc gagccaggcg tgacgctgct ggaagcggtc cagccgctcg agcacggccc
     2761 tcatcctacg gccacgctgc cgatctgccc gatggaggca gcggcaactt cgacatctgg
     2821 ttccggtgcg acgatggagt cagcgacctg tacgtgacgg gcagtttcga gaacatcact
     2881 gacccggatg tgatcaactg ctgatcggga ggcgcgatgc gtggaatcct tctcggtgtg
     2941 gtcgctgcag gaatcctgac cgtcggagcg atcggcgtgg tggctcagac tcccgacgcc
     3001 acggtcatca aggagtctga gatcgagcag aacgaaccgc atctcgtcga cgcggcgaac
     3061 gatcccgagc ttcagaggct cgctcgggac atcaacgaga cccagcgtgc ctgcgccgcc
     3121 tcggccgaag cgtgggccaa ctgtcccgtc gacgagctgc gggtcatgcg cgcagagctc
     3181 gagaagcaga tggagaggta cgcagcgaag taggcggccg agggccaccg ggatcacgcg
     3241 aagcgctcga cgagcgacat cgccaccggc gcgatcacga gggccgggag caggttcgcc
     3301 acgcggacct gcttcagctc cagcaggcgg aacccgatcc ccaggatcag cacgccaccg
     3361 gcggcggtca gggcggcgac gacgatcggc gtgaagacgc cggccagggc cgacgcgccg
     3421 agcgacaggc tcccctggta cacgaggatc gtcaggatcg agaaccccac cccccagccg
     3481 agcgccgagg cgaaggccag cgccgcgaac ccgtcgagca gcgccttgat cgccagcagg
     3541 tcgtagtcgc cgcgcagccc gtcctcgatc gagcccagga ccgtgagtgg gccgacgcag
     3601 aacaccagcg aggcgacgac aaagccttcg gtgaatcgcg cctggccctg cccgaagcgc
     3661 cgcttcagcc gctcgccggc ggactccagc cggtcctcga tccgcagcag ctccccgacg
     3721 atccccccgg ccagcacgct ccccaggatc accaccaggc tggcccggta gccggagagc
     3781 ggagcgcgga agacctcgaa ggcctcgccg gcgccgacca ccagcgtgac caggccgagc
     3841 gagtgcatca cggtctcgcg gatccggtcg ggcaggcggt tgccgaagag cgtcccgagg
     3901 acagtcccga ccaggaccgt cgcggcgttg atcagggtcc cggtgacgtg cacgagcgcc
     3961 gattctcgcc ccccggaccc ttgcgcgcga ccggcagttc ggcttcgatg tgcgccgtga
     4021 gcgaccgggc gatcggccac ctggacgagg tcctggacaa cctcggcgtg ttccacaccc
     4081 acccgcagga gctgctcgcc acctgcccgt cggggttccg ccgcgtgccg atcacccagg
     4141 gcgacggcta ctatatctcg ttccagggct tcgggccgga ccagccggcc ttcgcccaca
     4201 cccaccccga ctccgaggag tgggtgatcg tcctcgccgg cggcggctgg gcgctcgttg
     4261 acgagcatcc ggtgccgctc gagccgggct cgatggtcgg gcgggcgatg gcccacccgc
     4321 acggtttcgt ctccggtccg gaggggctgc agctgctctc ggtccagctt ccccggccgg
     4381 tcgagggggc gaccacctgg gacgccccgg gcgagaccac cgacccgatc gactgcgcgt
     4441 tcgggggccg gtgccggcgc tgctggcggt gcggcggcca cacccggcgg atggcgaccg
     4501 gccggttcag ctgcgagaac tgtacgctcg agttctgaac ggcacggggc ccgtgccggc
     4561 gctcagcccg cccgcttcca ctcctcgtcg ctccagtagg ttccgcagaa cgggcaggac
     4621 tcgccccggt gagcggtggg agcgacgacc cgtccacacc gagaacacgt cttcagcacg
     4681 ccgatggccg gtccggtcgt cgctttcgcc ttcggcgccc tgcccaggcg ccagccggcc
     4741 ttcttcaggt cgacgacctc cgggtgcgtg ttggtggccc tgcggacgcc tccccgcacc
     4801 ctgatgctcc ggctcgccag tcccgccagc acgcacgtca gcacgaagcc ccccccaccg
     4861 atcaaccagt tcccggcgac gagacccgcg gcgatcgccg cgggcacgga aaggagcacg
     4921 aggccgccca gccacagctc ggtgcgatga aggatcgcgg cgcaggccgt gcaggtcgcc
     4981 accgagacgg tccgttcgag gctccccgag tgcatcgtga ccttgcgagc cgatccgggg
     5041 tcctcggggt tcgtcccgca gtgccagcag cgctccacga tcgctcaccc ccaatgccgt
     5101 gcgacccacg gtagatcgcg gaaacgccag gcttcagggg gcgaagtacc catctccgag
     5161 gggaccgatg gcgtcatgcg gaggcgcccg ccgatctagg gttcgtctcg cgcgtcctgt
     5221 cggcggcccg ggccggcagc atcgtcctga tgcacgccct gccgcagacc gccgacgcgc
     5281 tcccgcagat catcgacgac ctgcaacgag cgcctctcca tggtgaccct gccccggctc
     5341 ttcgagaccg ccgacgacca gtagggccgg cggggcggcg gggccggtac cgggctcagg
     5401 cgacccgggg gggccggccg tcgtccgaga caagcggcag cccctcggcg aaccactcct
     5461 gcaggccgcg ctcgaggttg taagccacga acccgcgggc ccgcagcatc agcatggcca
     5521 cctcgctgcg gctgcccatc gagcacaccg cgacgaccgg ccgctccgga tcgaggtcgg
     5581 ccgggcccga catcaggcgg gacagcggga ggtgcaccga cccctcgatg tggccggccg
     5641 cccactcgtc ggcctcacgc acgtcgagca cctggacgtc gccgatccgg ccatgcaggc
     5701 cccgggaggt gctgatcgcg tcctcgatcc gctcttgact catggctcga tggtaccggc
     5761 tccgggatga agccgcccct gcagggtggc ccgcgctggg gctcgccctg gtctgcctct
     5821 cgctgctcgg cgcctggctc ctgccgctcg gctgagccaa caccgaacct aggaggaaac
     5881 gacctcctgg cagaactcgc cgaaggcgtc gacgtagcgg gcgatgtcgt cctcgtcgtg
     5941 ccgcaccgag atcgtccact gctcctcgtc gcccggcgtc atgaacacgc cgcggttgac
     6001 caggtagggc cacgacgcct cgaacagcgc gctgtcggtc tgcaggaagt cgcggtagcg
     6061 cttcaacggc g
//
LOCUS       DASPWO010000349         7588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_819754, whole genome shotgun sequence.
ACCESSION   DASPWO010000349 DASPWO010000000
VERSION     DASPWO010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819754"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VEO00_07965"
     CDS             <1..984
                     /locus_tag="VEO00_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57969.1"
                     /translation="ERPGVWGGSSPWRARHVTTPGERTPQGKKQEAARLARERAERRA
                     ARDFAAADALRERIAALGFTVTDTADGYELRRVPVPAVRRITPAEVPSALRDPPDHDA
                     TVHWLAEDWPEDVLRGIASFRAHAPGRSVQHVVVESVPAEVEWPERVEVLRLREDPGW
                     ARARNAGLPRTRGMIAVIADGSIEATGDWLGPLEGALANPTVGVTGPFGLVTEELHEF
                     VPSPGPECDAIEAYVLAVRRELLETGLAFDPRFRFYRSADIDLSFRVKDRGLRATVTP
                     LPLVQHEHRRWWSTPPEVRRKLSKRNYYRFLDRWRGRTDLTVAGTATPGTG"
     gene            complement(989..3031)
                     /gene="ftsH"
                     /locus_tag="VEO00_07970"
     CDS             complement(989..3031)
                     /gene="ftsH"
                     /locus_tag="VEO00_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HYU57970.1"
                     /translation="MAKNDNERRPRFPFDLGGGGGGGGNGSGAPNRRWRFSLGYVLIA
                     ALVLLLINALLFQGTEKVSFDRFLAFVDNEQVQQATISESSVTWTLKDGRTFSADIPP
                     NYDPTALVTDLAAKGVEVKGSQPSFWATALVSWVVPLVLIVLIYMFLFRRIAGGAASS
                     ALNLGRNRVKIYDRKDLKTTFADVAGVDEAVQELHEIVDFLRNPRKYQRLGGRIPKGV
                     LLLGPPGCGKTLLARAVAGEAKVPFFYMSGSEFVEMFVGLGAARVRELFQQAKEKAPC
                     LVFLDELDTIGKSRGGGLGGSFGTHDEREQTLNQLLVEMDGFDTSKGVIIMAATNRSD
                     VLDPALLRPGRFDRQVVVDRPDLRGREAILKVHARGVALDPSVDLTIIAARTAGFTGA
                     DLANAINEAALLAARREKNSVTMEELEEAIDRVVAGLERKSRVLSDKEREIVAVHEMG
                     HALVALQVPTADPLHRVSIIPRGAAALGMTMQRPLEDRYLLQEPELKDRLTILLGGRT
                     AEEIAFKVISTGAQNDLQRATEIARAMVIEYGMSERLGPISFGQDGFRTAEGRLYFPG
                     AGPEISVEVQNLVDAETSRLVEEAHDRARTILEGAGDLLPRLSKVLMAKEVIEGKELK
                     AYLERTEPIPSDEELERIRHERAPAPDAPTAPITPSPTAPVAPSLPPPPAAASSAD"
     gene            3098..3715
                     /locus_tag="VEO00_07975"
     CDS             3098..3715
                     /locus_tag="VEO00_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU57971.1"
                     /translation="MATRGAQERIPQVGGPDERSGEDLRREFEEQAFPLMPGLYGAAL
                     RLTRNPADAEDLVQETFLRAFRGFRTFEPGTNLRAWMYRILTNTFINSYRKRQREPQT
                     IPDDGVEEWYLYNRLAREAPDSSAEAAVLESLPDEDVQAALEALPDGFRTAVLLADVE
                     GFSYKEIAEIMGVPIGTVMSRLHRGRKALQKALFETMRERGLVRD"
     gene            3726..3959
                     /locus_tag="VEO00_07980"
     CDS             3726..3959
                     /locus_tag="VEO00_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYU57972.1"
                     /translation="MLRQVERYLDGELDVLHVQELEVHVQGCSPCLDRLEFQRALKAL
                     IAEKCGCREVPAALFERIRRALPRHDPPATPIV"
     gene            4057..4245
                     /locus_tag="VEO00_07985"
     CDS             4057..4245
                     /locus_tag="VEO00_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57973.1"
                     /translation="MKGTRPGHIPPPSRTAEQNGRTCAHRGCVTTLSRYNLGSHCWQH
                     TDLVFPNYRGKRLAEGKT"
     gene            4407..4604
                     /locus_tag="VEO00_07990"
     CDS             4407..4604
                     /locus_tag="VEO00_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYU57974.1"
                     /translation="MAEKRNAPSKYLKTAEVAELLHVSPKTVTRWAKDGKLPHSRTLG
                     GHRRFPGEAIRKLADDLIETV"
     gene            4669..5988
                     /locus_tag="VEO00_07995"
     CDS             4669..5988
                     /locus_tag="VEO00_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HYU57975.1"
                     /translation="MFGRVLVANRGEIAVRVLRTLHELGIDGIAVYSDADANALHVRQ
                     ADGAVRIGESPAPLSYLNPSAVVQAALDSHCEAVHPGYGFLSENAMFARACEQAGLAF
                     IGPPPAVLELTGDKVAAREAFAGAGFPVLPGGGPYGTGQEALAAVAGIGYPVMVKAVM
                     GGGGIGMGVARDDAELAKQVETASARGARFFADPAVYLERYVDGARHVEVQVLFDRHR
                     EGTHLYERECSVQRRHQKVIEEAPSPAVDDALLERMAIAALEAMRLIGYQVAGTVETI
                     LTPKGEFFFLEVNARLQVEHCVTEETCGVDLVEAQVRGASGEPLASLGLPAGRAGHAI
                     ECRVYAEDPDTFLPSPGTIEKLELPEGVRCDFGYEAGDEVPMFYDPLIGKVVAHAEDR
                     PATIAAMRSALEALVLEGPKTNIETHLRVLDDDRFVAGDYDTGILGR"
     gene            5985..6749
                     /locus_tag="VEO00_08000"
     CDS             5985..6749
                     /locus_tag="VEO00_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYU57976.1"
                     /translation="MSVRFEVRGAGAWLTIDREERRNALSPEVIRELIECLARATQDE
                     DVRAVVVTGAGEKAFCAGGDIGGFAGGEAVETAPAAIGRLLDLIWHHPKPTIARVNGR
                     ALGGGFGLLLACDVAIAADDVEVGMPEIDIGLWPHVITAVVQRRVPRNVALELMLTGR
                     RVGAEEAARWAMVNRIVPRAELDRAVEDLVATIAAKSPAIIRLGKRSFAGTEDRRFGD
                     AIAFLKEMLAENLEAPDLAEGVAAFLQKRRPDWTGR"
     gene            6839..7423
                     /locus_tag="VEO00_08005"
     CDS             6839..7423
                     /locus_tag="VEO00_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU57977.1"
                     /translation="MATIENVLYLPDPQSFADMQSGDMVRTRRARKAMDRGSISTEGG
                     ALRFQGAKGTVELSPVRGVTHDRWAKVEYGTGADIRTGYFSVPSKIAGKKNRLLVQSL
                     QQALGLTEAAPGQDVELDRHRSQVRAATARRSLIQMWIGGAVAVIGILVTVITLSAAS
                     GSSGGRYIVAWGAILFGGLAFVQGLVEWRRNRGG"
BASE COUNT         1081 a         2673 c         2790 g         1044 t
ORIGIN      
        1 gagcgaccgg gggtttgggg gggtagctcg ccctggcggg cgcggcacgt gaccacgccc
       61 ggcgagcgaa ccccccaagg caagaagcag gaggcggcgc gcctggcacg ggagcgggcc
      121 gaacgacgcg ccgcccgcga cttcgcggcc gcggacgccc tgcgcgaacg catcgccgcg
      181 ctcggcttca ccgtcacgga cacggccgac gggtacgagc ttcgccgcgt cccggtcccc
      241 gccgtccggc ggatcactcc cgccgaggtc ccctcggcgc tccgggatcc acccgaccac
      301 gacgccaccg tgcactggct cgccgaggac tggcccgagg acgtgctgcg ggggatcgcc
      361 tcgttccggg cgcacgcccc cggccggagc gtgcagcacg tcgtcgtcga gtcggtcccg
      421 gccgaggtcg agtggccgga gcgggtcgag gtgctccgcc tgcgcgagga tcccgggtgg
      481 gcccgcgccc gcaacgccgg cctgccacgg acccgcggca tgatcgccgt gatcgccgac
      541 ggctcgatcg aggccaccgg cgactggctg ggcccgctcg agggcgcgct cgccaacccg
      601 acggtcgggg tcaccgggcc gttcgggctg gtcaccgagg agctgcacga gttcgtcccc
      661 tcgccggggc ccgagtgcga tgcgatcgag gcctatgtcc tggccgtccg ccgcgagttg
      721 ctggagacgg ggctggcctt cgacccgagg ttccgcttct accgctcggc cgacatcgac
      781 ctgtcgttcc gggtgaagga ccggggcctg cgggcaaccg tcacgccgct cccgctggtg
      841 cagcacgagc accgccggtg gtggagcacg cctcccgagg tccgccggaa gctgtcgaag
      901 cgcaactact accggttcct cgaccgctgg cgcggcagga ccgacctgac ggtggccggg
      961 acggcaacgc cggggacggg ctgagcggtt agtccgcgga gctcgcggcg gccggaggag
     1021 gcggcaggga gggcgccacg ggcgcggtgg gcgacggcgt gatcggcgcg gtcggcgcgt
     1081 ccggcgcggg cgcgcgctcg tgccggatgc gttcgagctc ctcgtcggag gggatcggct
     1141 cggtccgctc gaggtaggcc ttgagctcct tgccctcgat gacctccttg gccatgagca
     1201 ccttggacag ccgcggcagg aggtctccgg cgccctccag gatggtccgc gcgcggtcgt
     1261 gcgcctcctc gaccaggcgg ctcgtctcag cgtcgacgag gttctgcacc tccacggaga
     1321 tctccggccc ggcgccgggg aagtacagcc ggccctccgc cgtgcggaac ccgtcctggc
     1381 cgaacgagat cggcccgagc cgctcgctca tgccgtactc gatgaccatc gcccgggcga
     1441 tctcggtcgc ccgctgcagg tcgttctggg cgcctgtcga gatgaccttg aaggcgatct
     1501 cctccgcggt gcggccgccg agcaggatcg tcaggcggtc cttcagctcg ggctcctgca
     1561 gcaggtaccg gtcctcgagc ggccgctgca tggtcatccc cagggcggcg gcccctcgcg
     1621 ggatgatcga gacccggtgc aggggatcgg ccgtgggcac ctgcagggcg acgagggcgt
     1681 ggcccatctc gtggacggcc acgatctcgc gctccttgtc ggacagcacg cggctcttac
     1741 gctccaggcc ggccacgacc cggtcgatgg cctcctccag ctcctccatg gtcacggagt
     1801 tcttctcccg ccgggcggcc agcagcgcgg cctcgttgat cgcgttcgcc agatcggcgc
     1861 cggtgaaccc cgccgtgcgc gcggcgatga tggtcaggtc gaccgacggg tcgagggcca
     1921 cgccccgcgc gtgcaccttg aggatcgcct ctcgcccgcg caggtcggga cggtcgacga
     1981 cgacctgccg gtcgaaccgg cccggccgca gcagcgccgg gtcgagcacg tccgagcggt
     2041 tggtcgcggc catgatgatc acgcccttgc tggtgtcgaa cccgtccatc tcgaccagca
     2101 actggttcag ggtctgctcg cgctcgtcgt gggtgccgaa cgacccgccg agcccgccgc
     2161 cgcgcgactt gccgatcgtg tccagctcgt cgaggaacac caggcacggg gccttctcct
     2221 tcgcctgctg gaacagctcg cgcacccgcg ccgcgccgag cccgacgaac atctcgacga
     2281 actcggagcc cgacatgtag aagaacggca ccttggcctc accggccacg gcccgcgcca
     2341 ggagggtctt gccgcagccg ggcgggccga gcagcagcac ccccttcggg atgcgcccgc
     2401 cgaggcgctg gtacttccgg gggttgcgca ggaagtccac gatctcgtgg agctcctgca
     2461 ccgcctcgtc cacgccggcc acgtcggcga acgtcgtctt gaggtccttg cggtcgtaga
     2521 tcttgacgcg gttgcggccc aggttgaggg cgctggaggc cgcgcccccg gcgatccgcc
     2581 ggaacaggaa catgtagatc agcacgatga gcacgagcgg gacgacccag ctgacgagcg
     2641 cggtggccca gaacgacggc tggctgccct tcacctcgac gcccttggcg gccaggtcgg
     2701 tgacgagggc cgtcgggtcg tagttcggcg ggatgtcggc ggagaaggtg cggccgtcct
     2761 tcagcgtcca cgtcacgctc gactcggaga tcgtcgcctg ctggacctgc tcgttgtcga
     2821 cgaaggcgag gaagcggtcg aacgacacct tctccgtgcc ctggaacagc agcgcgttga
     2881 tcagcaggag caccagcgcg gcgatcagta cgtagccgag cgagaagcgc cagcgccggt
     2941 tcggcgcgcc ggagccgttg ccgcccccgc cgcccccgcc gccgagatcg aacgggaagc
     3001 gcggccgccg ctcgttgtcg ttcttcgcca tgtgggatca atcatcccac cgccgccggg
     3061 aacacgcccg gtggtggcct ccctctatgc tctgtcggtg gcgacgcgcg gcgcgcagga
     3121 gcggatcccc caggtcggcg ggccggacga gcggtccggg gaggacctgc gccgggagtt
     3181 cgaggagcag gccttcccgc tgatgcccgg cctgtacggg gccgccctgc gcctgacccg
     3241 caatccggcc gacgccgagg acctggtcca ggagacgttc ctgcgggcgt tccgcggctt
     3301 ccgaacgttc gagccgggga cgaacctccg ggcctggatg taccgcatcc tgaccaacac
     3361 gttcatcaac agctaccgca agcgccagcg cgagccgcag accatccccg acgacggtgt
     3421 cgaggagtgg tacctgtaca accggctggc ccgcgaggcc cccgactcct cggccgaggc
     3481 ggccgtgctc gagtccctgc ccgacgagga cgtccaggcg gcgctcgagg ccctgccgga
     3541 cggcttccgc acggccgtgc tgctcgcgga cgtcgagggc ttctcctaca aggagatcgc
     3601 cgagatcatg ggcgtgccga tcggcacggt gatgtcgcga ctgcaccgcg gaagaaaggc
     3661 gctgcagaag gcgttgttcg agacgatgcg ggagaggggc cttgtccggg actgattgca
     3721 ctgccgtgct tcgccaggtc gagcggtatc tcgacgggga gctcgacgtc ctgcacgtgc
     3781 aggagctcga ggttcacgtc caggggtgca gcccgtgcct ggaccggctg gagttccagc
     3841 gcgccctgaa ggcgctgatc gccgagaagt gcggctgccg tgaggtgccg gccgccctgt
     3901 tcgagcggat ccgccgggcc ctgccgcgtc acgacccgcc ggcaacgccc atcgtctgat
     3961 cccggggggg cccggggccg gcctcggcga accccccgag cgtgggcggc ggattccttt
     4021 ccaaggaggg gccggcggtg accggcccgg cactcgatga agggcacccg acccggtcac
     4081 atccccccac cgtccaggac cgccgagcag aacggtcgca cctgcgccca ccggggctgc
     4141 gtcacgacgc tctcccgcta caacctgggc tcacactgct ggcagcacac cgacctggtg
     4201 ttcccgaact accgcggcaa gcgactcgcc gagggcaaga cctagtcggg gcttcccccc
     4261 cgactacccc cgaaaggccg ggacgaacgg gacggaatac cagaaagcag acggccctga
     4321 cgggccgtac ccattcactt ttgtcggacg atctggtaac aatccaagga acggatcgtc
     4381 cggatcccat gcccgggggg cgagagatgg ccgagaagcg aaacgccccg tccaagtacc
     4441 tgaagacggc agaggtggcc gagctcctcc acgtctcgcc gaagaccgtg acgcgctggg
     4501 cgaaggacgg caagctgccc cactcgcgca cgctcggcgg tcaccggcgg ttccccggcg
     4561 aggcgatccg caagctcgcc gacgacctca tcgagacggt ctgacgcccg ccgccgctgg
     4621 gagcgcgccg ggggcggccc tggggcccgc ccgctaagct cccgcctcgt gttcggcagg
     4681 gtcctggtgg cgaaccgcgg cgagatcgcc gtgcgcgtgc tgcggacgct ccacgaactg
     4741 gggatcgacg ggatcgccgt ctactccgac gccgacgcga acgcgctgca cgtgcgccag
     4801 gccgacggcg cggtccggat cggcgagtcc cccgccccgc tctcctacct caacccctcg
     4861 gccgtggtgc aggccgcgct cgacagccac tgcgaggccg tgcaccccgg ctacgggttc
     4921 ctgtccgaga acgcgatgtt cgcccgcgcc tgcgagcagg ccggcctggc gttcatcggc
     4981 ccgccgccgg cggtcctcga gctgaccggc gacaaggtcg ccgcccggga ggccttcgcc
     5041 ggggccggct tcccggtgct tcccggcggg ggaccgtacg gaacggggca ggaggcgctc
     5101 gcggcggtcg ccggcatcgg ctatccggtg atggtcaagg ccgtgatggg cggcgggggc
     5161 atcgggatgg gggtggcgcg cgacgacgcg gagctcgcga agcaggtcga gaccgcctcc
     5221 gcgcgcgggg cgcggttctt cgccgacccg gccgtctacc tggagcgcta cgtcgacggc
     5281 gcgcggcatg tggaggtgca ggtcctcttc gaccggcacc gcgagggcac ccacctctac
     5341 gagcgagagt gctcggtcca gcgccgccac cagaaggtga tcgaggaggc accgtcgccg
     5401 gcggtcgacg acgccctgct cgagcggatg gccatcgcgg cgctcgaggc gatgcggctg
     5461 atcggatacc aggtcgcggg gacggtcgag accatcctca cgccgaaggg tgagttcttc
     5521 ttcctggagg tgaacgcccg gctgcaggtg gagcactgcg tgacggagga gacctgcggc
     5581 gtcgatctgg tcgaggccca ggtgcgcggg gcgagcggcg agcccctggc gtcgctcggc
     5641 ctgccggccg ggcgagccgg ccacgccatc gagtgccgcg tctacgccga ggacccggac
     5701 acgttcctgc catcacccgg cacgatcgag aagctcgagc tgccggaggg cgtgcgctgc
     5761 gacttcggat acgaggccgg ggacgaggtc ccgatgttct acgacccgct gatcgggaag
     5821 gtggtcgcgc acgccgagga ccggcccgcc acgatcgccg cgatgcgctc cgcgctggag
     5881 gcgctggtgc tcgagggccc gaagacgaac atcgagaccc acctgcgcgt gctggacgac
     5941 gaccgcttcg tcgccggcga ctacgacacc gggatcctgg ggcgatgagc gtccggttcg
     6001 aggtgcgcgg ggcgggtgcg tggctgacga tcgaccggga ggagcggcgg aacgcgctct
     6061 cgccggaggt gatccgcgag ctgatcgaat gcctggcgcg ggcgacgcag gacgaggacg
     6121 tccgcgcggt cgtcgtcacc ggcgcggggg agaaggcgtt ctgcgccggc ggggacatcg
     6181 gcgggttcgc cgggggtgag gccgtggaga cggctccggc ggcgatcggg cggctgctcg
     6241 acctgatctg gcaccatccg aagccgacca tcgcccgggt gaacggccgg gccctcggcg
     6301 gcgggttcgg tctgctgctg gcctgcgacg tggcgatcgc cgccgacgac gtcgaggtcg
     6361 ggatgcccga gatcgacatc gggctgtggc cgcacgtgat caccgccgtg gtgcagcgtc
     6421 gcgtgccgcg caacgtggcc ctcgagctga tgctcaccgg ccgccgggtg ggtgccgagg
     6481 aggccgcgcg gtgggcgatg gtcaaccgga tcgtcccgcg cgccgagctc gatcgggccg
     6541 tcgaggacct ggtggccacg atcgccgcga agagcccggc gatcatccgg ctcgggaagc
     6601 gctcgttcgc cggcaccgag gaccgcaggt tcggcgacgc gatcgcgttc ctgaaggaga
     6661 tgctcgccga gaacctcgag gcccccgacc tggccgaggg cgtggcggcg ttcctgcaga
     6721 agcgccgccc cgactggacc ggccgctgag gcggacattc cgcgaaaccc ttgcgaggcg
     6781 tgacggccac ctcctacagt tgatccggga ggccagcgcg tcgcagcagg aggtcgccat
     6841 ggccacgatc gagaacgtcc tctatctccc ggacccgcag agcttcgcgg acatgcagag
     6901 cggggacatg gtccggacca ggcgggcgcg gaaggcgatg gaccgggggt cgatctcgac
     6961 cgaagggggc gccctccggt tccagggtgc caagggcacc gtcgagcttt ccccggtccg
     7021 cggcgtcaca catgaccgct gggcgaaggt cgagtacggg acgggcgccg acatccgtac
     7081 gggttacttc agcgttccgt ccaagatcgc gggcaagaag aaccggctcc tggtccagtc
     7141 cctccagcag gcgctcgggc tgaccgaggc ggcaccgggc caggacgtcg agctcgatcg
     7201 tcaccgctcc caggtccggg ccgccacggc gaggaggtcc ctgatccaga tgtggatcgg
     7261 cggcgcggtc gccgtgatcg ggatcctcgt caccgtgatc accttgagcg cggcgtccgg
     7321 ctcgagcggc ggccgctaca tcgtcgcctg gggggcgatc ctcttcggcg ggctcgcgtt
     7381 cgtgcagggc ctggtggagt ggcggaggaa ccgcgggggg tagcgacgcg ccgcggagcc
     7441 cgttgatgcc cgaacggcct cccctcgtcc acatcctcga gggcatcgag cgggacgacc
     7501 cctcgggcgg cgctccggag cgcggccggc tggaggccgg cgagggcttc ctggagttcc
     7561 gcggggaggc ggcgagcctg cggatgac
//
LOCUS       DASPWO010000350         2783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_819888, whole genome shotgun sequence.
ACCESSION   DASPWO010000350 DASPWO010000000
VERSION     DASPWO010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819888"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VEO00_08010"
     CDS             complement(<1..617)
                     /locus_tag="VEO00_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYU57978.1"
                     /translation="MPDHDDAAAALDLLSGLHHLIGSGSGLDELASGTAGTLVRDYGA
                     RAAQVWTHPAGGSPALAARAGAERPLPPGALEGVAAGSSVTDPGGEHLTLASAEVADA
                     VAVVAVWTDERPRGAWRRVLDLFAEHVAIGLADDRRRAESTRAVGELARTASSLAALV
                     EATDAGILMVDTEGRLEFANERVERILDIPLRSMIGQPAQRLMAE"
     gene            complement(669..1085)
                     /locus_tag="VEO00_08015"
     CDS             complement(669..1085)
                     /locus_tag="VEO00_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HYU57979.1"
                     /translation="MRPRVAAERGSASVLAAALAGLAMALALFTVDLARVVVARSRAQ
                     AAADAAALAAAAELARPSGRSPPSVAAEYAELGGAHLVECRCGPGTAEAVVTVELRVG
                     LGALGGARTVTARARAIIAPAADGDSRTAPIDGFRK"
     gene            complement(1090..1527)
                     /locus_tag="VEO00_08020"
     CDS             complement(1090..1527)
                     /locus_tag="VEO00_08020"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HYU57980.1"
                     /translation="MRPPSSRRVGAAGSGETDRPPAERGTSTVEFALVLPLVLVVALA
                     LMQVGLFLVDQLIVVEAARSGAREAAVTTDEARVRGAALRAGPWLAEERTAVAVDRTG
                     GIGDPVTVRVRYRAEIVVPLVDWLFPGSVELEGAATMRQEMES"
     gene            complement(1524..1715)
                     /locus_tag="VEO00_08025"
     CDS             complement(1524..1715)
                     /locus_tag="VEO00_08025"
                     /inference="COORDINATES: protein motif:HMM:NF025395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HYU57981.1"
                     /translation="MTRLFVLLRGRLVREAGQTTAEYALVILAAAAIAVVLIAWARSS
                     GKLPAFFDHVIDQITGQAE"
     gene            complement(1739..2599)
                     /locus_tag="VEO00_08030"
     CDS             complement(1739..2599)
                     /locus_tag="VEO00_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYU57982.1"
                     /translation="METLFAGWFAVAVLGSGLAASVRADRRVGGRLGEQATLRPPAEG
                     SPGAVARLAAGLERSRAFDRHLVAQRLALAGRPAGTREHAEARVRAALAAAACGVLLS
                     LAAPEGLAALPILVVAGLRWPDVRLARAARARQDAIARAAPDLLDLVACCVAAGLSPP
                     AGLDRAGARLGGPLGEEVRAALHLVAVGRPWRGALAETLGRTGVPSLRRLVSAFERTD
                     RLGATLAPVLVSLAREARAERRAAAEERARAAPVKLLFPLVLLILPAFLLLTITPVVL
                     ATLRGLSGGP"
BASE COUNT          395 a         1094 c         1017 g          277 t
ORIGIN      
        1 atctcggcca tcagccgctg ggcgggctgg ccgatcatcg agcgcagcgg gatgtccagg
       61 atccgctcga cccgctcgtt ggcgaactcc aggcggccct cggtgtcgac catcaggatc
      121 ccggcgtcgg tcgcctccac cagcgccgcc agcgagctcg ccgttcgcgc cagctcaccc
      181 accgctctgg tggactcggc ccgccggcga tcgtcggcca gcccgatcgc cacgtgctcg
      241 gcgaacaggt cgaggacccg gcgccaggct ccccgcggac gctcgtcggt ccagacggcg
      301 acgaccgcga ccgcgtcggc gacctcggcg gaagcgagcg tcaggtgctc cccgccaggg
      361 tcggtgacgg agctcccggc ggccacccct tccagggcgc cgggcggcag cggccgttcg
      421 gcacccgccc gcgccgccag ggccgggctg cccccggccg ggtgggtcca gacctgcgcc
      481 gcgcgagcgc cgtagtcgcg cacgagtgtc ccggccgtgc cggacgccag ttcatcgaga
      541 cccgatcccg atccgatcag gtggtgcaac cccgacagca ggtcgagcgc tgcagcggcg
      601 tcgtcgtggt ccggcatcgt gcgtcgtggc gcggttcggt gagcgccgcc gtgctgcgaa
      661 cataggcatc atttcctgaa cccgtcaatc ggagccgtgc gcgaatcgcc gtccgccgcg
      721 ggtgcgatga tcgcccgggc gcgggccgtc acggtacgcg cccccccgag cgcgcccagg
      781 ccgacacgaa gctcgacggt caccaccgcc tcggccgtcc cgggcccgca gcggcactcc
      841 accaggtgcg cgccaccgag ctcggcgtac tcggccgcga ccgacggggg cgaccgcccg
      901 gacggccgcg ccagctccgc cgcggcggca agggctgcgg cgtcggccgc ggcttgggcg
      961 cggctccgag ccacgacgac tcgcgccagg tcgaccgtga agagggcgag cgccatcgcc
     1021 aggccggcca gggcggcggc cagcactgac gccgacccgc gctcggcggc gaccctcggg
     1081 cgcatcggct cagctctcca tctcctggcg catcgtcgcc gcgccctcga gctcgacgct
     1141 cccggggaac aaccaatcga cgagcggaac gacgatctcc gcgcggtaac ggacgcggac
     1201 cgtgaccgga tcgccgatcc cgcccgtgcg gtccacggcc acggcggtcc gctcctcggc
     1261 cagccacggc ccggcgcgga gcgcggcccc tcgcacccga gcctcgtcgg tcgtcacggc
     1321 ggcctcccgt gcgccggaac gcgccgcctc gacgacgatc aactggtcga ccaggaacag
     1381 accgacctgc atgagcgcga gcgcgaccac cagcaccaga gggaggacga gggcgaactc
     1441 cacggtggag gtccctcgct cggctggggg gcggtcggtc tcgccgctgc cggcggcgcc
     1501 gacccggcgc gacgacggcg gtctcattcg gcctgaccgg tgatctggtc gatgacgtgg
     1561 tcgaagaacg ccgggagctt cccggacgag cgcgcccagg cgatcaggac caccgcgatc
     1621 gcggcggccg ccaggatgac cagcgcgtac tccgccgtgg tctgccccgc ctctcggacg
     1681 aggcgccccc gcagcaacac gaacagccgt gtcacgaccc acctcctttc gctcctcgct
     1741 acggcccgcc cgacagcccg cgaagggtgg cgagcacaac cggcgtgatg gtgaggagga
     1801 ggaaggcggg gaggatcagc agcaccagcg ggaagagcag cttcaccggc gcggcgcgtg
     1861 cgcgctcctc ggccgcggcg cgacgctcgg cccgcgcctc gcgcgccagg gatacgagca
     1921 ccggggccag cgttgccccc agccggtcgg tccgctcgaa cgccgagacc aggcggcgga
     1981 gggacggcac cccggtgcgc cccagcgtct cggcgagcgc ccctcgccag ggccggccga
     2041 ccgccaccag gtgcagggcg gcccgcacct cctccccgag cgggccgccc aaccgggccc
     2101 cggcgcggtc cagccccgca gggggcgaca ggccggccgc cacgcagcag gccaccaggt
     2161 cgagcaggtc gggggcagcg cgggcgatgg catcctggcg cgctcgcgcc gcccgggcca
     2221 ggcggacgtc gggccaccgc agtccggcga ccacgaggat cgggagtgcg gccagtccct
     2281 cgggcgcggc gagcgacagg aggacaccgc aggcggcagc ggcgagcgcg gcacggaccc
     2341 ttgcctcggc gtgctcgcgc gtcccggccg gccgtcctgc aagcgccaac cgctgcgcca
     2401 ccaagtgccg gtcgaaggca cgggagcgct ccaggcccgc agcgaggcgc gcgaccgcgc
     2461 cgggtgaccc ctcggccggc gggcgcaacg tcgcctgctc gccgagccgg ccgccgacgc
     2521 ggcggtcagc gcgcaccgag gccgccagcc cggagccgag gaccgcgacc gcgaaccagc
     2581 cggcgaacag ggtctccacg gaaggctcag acctccaccc ggacgatccg gcggacccac
     2641 aacgccccga gcgtctgcag gaggagcccg gtcccgacga gcgccgcccc ggtcgggctg
     2701 agcaggactg ggacgagctc gtccccggag gtcagtgcgg tcagggcgaa gaacgccgcc
     2761 ggaagcacgg tgaggatgcg gcc
//
LOCUS       DASPWO010000351        10095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1152815, whole genome shotgun sequence.
ACCESSION   DASPWO010000351 DASPWO010000000
VERSION     DASPWO010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10095
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152815"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VEO00_08035"
     CDS             <1..302
                     /locus_tag="VEO00_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYU57983.1"
                     /translation="RTLFDAGAKYCYAQGTSMAGPHVVGVAALIASRYGPLAPGELAA
                     FVEQSAESLACPDPYEPQGTDFPRLDGSPQECQGGTGNNSFYGAGEVNAYNAVTL"
     gene            376..1926
                     /locus_tag="VEO00_08040"
     CDS             376..1926
                     /locus_tag="VEO00_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HYU57984.1"
                     /translation="MKKLDLDESLVQEARDLARSIARPVEEYIAGHTTVAIERAVLRL
                     LGLDGAVGEGMESRPFPNLVVDAVREQLGLGRGVAIPVFDAMARESLSIHQVGAAVAV
                     GELTFRLPPDETEVRETIAAAAREADGRILARRRERDELVHHLGDPEKPWIYLIVATG
                     NIHEDIPQAQAAARAGADVIAVIRSTGQSLLDYVPYGATMEGFAGTFATQENFRLMRR
                     ALDETSQEIGRYVRLTNYASGLCMPEIAAMAGLERLDMMLNDCMYGIIFRDINMRRTF
                     VDQRFSRMLHARAGIIINTGEDNYLTTQDPVEGARAVVASQLLNERFALDAGLEPWQL
                     GLGHAFEIDPDRPDAWLFELAHAQLVRELFPEAPLKYMPPTKHMTGDVFMGYLLDGMF
                     HLTGVLTEQDILLIGMMTEGIHTPFLSDRDLAIQNARYMVRTARNVAAEWWPPHEGRI
                     RARARQVLEETVAHLRRVDDLGMFEAIQAGEFADTSRPIDGGHGLDGVVRKDEGYLNP
                     FLEIWEAP"
     gene            1923..2687
                     /locus_tag="VEO00_08045"
     CDS             1923..2687
                     /locus_tag="VEO00_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HYU57985.1"
                     /translation="MTEADVRHVVRPYGDDTDDGKVQVSFTLPIPSDAKAEAAARELA
                     GKMGMRSPLVVHRKAIGRLFTFFIVYGTVDHAVDLDAIRIEERPFPLLDHTDVRRRIR
                     QALGREAVVVGAALGTDAHTVGLDAILSLKGIAGDKGLESYAAFRVVNLRSQVRPEDL
                     VRRSLQEGADAVLISQTVTQKDAHLHHLREFADEVDREGARGRFLMIAGGPRLTPDMA
                     ADLGYDRVFGKGTTPTEVASYLAWALPSAGGGVHER"
     gene            2677..3123
                     /locus_tag="VEO00_08050"
     CDS             2677..3123
                     /locus_tag="VEO00_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HYU57986.1"
                     /translation="MSDEFPQAVLRIRLSQTDAHYGGNLVPGSRIVELFGDLATELCV
                     RMDGDEGLFAAYEEISFTAPVHAGDFLEVRGRIIGAGQTSRRMAFEARKYVIPRPEIS
                     ESAAEVLEEPLVVARAIGTCVVQTTRQRGRAATRLARDVEHLFDEG"
     gene            3132..3968
                     /locus_tag="VEO00_08055"
     CDS             3132..3968
                     /locus_tag="VEO00_08055"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018255909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYU57987.1"
                     /translation="MPEDIPGYLASNRALWDEWTAIHETSSFYDLEGFKAGGVRLRPY
                     EVEEVGDVAGKDLLHLQCHFGIDTISWARLGARVTGADFSERGIALARRVAEEIGIAA
                     RFVHSNVYDLPDAPELRDERFDVVYTSRGVIGWLPDLFPWARVVAHFLKPGGLFYVTE
                     GHPTMWVFDDAEDVEGLQVAYPYYARPEPLAFEVQGSYAEPTAAVKSAMEYSWPHSMG
                     EIVTAIAEAGLVIEFLHEFPFLDWPMPFLEDQGDGTWRLPASQAGEIPLTFSLRAHKP
                     WE"
     gene            complement(3993..5987)
                     /locus_tag="VEO00_08060"
     CDS             complement(3993..5987)
                     /locus_tag="VEO00_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYU57988.1"
                     /translation="MPNRLADATSPYLLQHAANPVDWYPWGEEAFAKARAEDKPVLLS
                     VGYAACHWCHVMERESFEDPPTARLMNEHFVSIKVDREERPDVDAIYMEAVQAMTGGG
                     GWPMTVFLTPGGTPFYAGTYFPNEDRHGMPALSRVLMALAEAWRDDRERVVAQGERVV
                     AAIARTPDESREPLTEDLLRGAYRSLERSFDPEWGGFGPAPKFPQPMNLELLLRTHLR
                     GYPGALDMATRTLERMASGGMYDQVGGGFARYSTDRMWLVPHFEKMLYDNAQLARLYL
                     HAWQVTGTGWYAAVVRETLDYLLREMRAPEGGLYSAQDADSEGVEGKFYLWPYDELRA
                     VAGEDVARWLGALPDGNREGTNILWRAGGDPSPADWPAARERLYAAREERVRPATDDK
                     VLAGWNGLAIQALAEAGRALGEPRYVEAAGAAAGFVLERMRREDGRLLRAWRDGRTSG
                     PAYAEDHAILAAALLVLHETTFDLRWLAEARALADELVRLFRDRDGGGFFQTGADAEA
                     LVVRPKDLFDNAVPSGNSVAAEVLLRLHLLSGEAEYERAAVSALRVARPLMERASGAA
                     GHALQALDLYLSPAREVAVVGEPAAADTHALAAEVWRRFLPNVVLAVARPGDPDAAKV
                     APLLEGREARDGRATAYVCARFACRRPVTDPAALAEQLAS"
     gene            6124..6504
                     /locus_tag="VEO00_08065"
     CDS             6124..6504
                     /locus_tag="VEO00_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU57989.1"
                     /translation="MDTPLMAMLPASDYERAKAWYRDKLGLTPVRDEPEGADYEIGGV
                     RFGVYPSQFAGTNQATAAGFYVDDIEKTIDELTAKGVVFEQYDMPGLKTDEKGIAELG
                     PFKGAWFKDSEGNILVVSEEAPPS"
     gene            complement(6501..7070)
                     /gene="mug"
                     /locus_tag="VEO00_08070"
     CDS             complement(6501..7070)
                     /gene="mug"
                     /locus_tag="VEO00_08070"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HYU57990.1"
                     /translation="MGWPVRAAEPGPDALTTVPDVLEPGLRVLFVGINPGLRSAEVGH
                     HFARPGNRFWKALHGAGFTDRVLGPAEDGSLPAWGIGITNLVARPSGNADEITRQELR
                     AGARRVERLVGRYRPAAVAFLGVGAYRAAFGRPRAAVGAQPERLAGAPVWVLPNPSGR
                     TAAYQVPDLVRLFGELRAAVDSGGVGGSA"
     gene            complement(7025..7573)
                     /locus_tag="VEO00_08075"
     CDS             complement(7025..7573)
                     /locus_tag="VEO00_08075"
                     /inference="COORDINATES: protein motif:HMM:NF025382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4232 domain-containing protein"
                     /protein_id="HYU57991.1"
                     /translation="MRGRSGRRSAAIVAALLAVSTLAGAAPTAHAGVPPFCTPDMLRG
                     RFDLTGAAGSLVGQIVVKNASYSECTVKGRSRLIPVNANGEAYPVEQNSVPPYWRSLG
                     QPPPPGWPNVVLVPDGRAWVRFAWANWCFMNPPDRWRMVLPWGGARRIPAIDSTPPCN
                     QPGAKSMVWVGPFEPPNPAPTH"
     gene            7685..8569
                     /locus_tag="VEO00_08080"
     CDS             7685..8569
                     /locus_tag="VEO00_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HYU57992.1"
                     /translation="MTRRVLLDTSSLMYRAFFALPPQIRAADGTPANAVHGYLNWTAA
                     LIQSRATGDLIHCYDHDWRPASRVAEYDGYKATRQVDPGNLPDQFAWLREVLDAIGMA
                     QAEAEGWEAEDAIGSIAARARGRDRIDIVTGDRDLLQLVRDPAVTLWFTRKGVTEMDR
                     YDAAAVLAKYGIPPSRYAEFAILRGDPSDELPGVKGIGEKTARELVNAYPDLDALLAA
                     ARSDPRAKLTPRIRSALIDSAGYVAAMQRIVPIRTDLEVRVWTPVPDPDRAEELADRY
                     RVGGPLRRIREALDAVGR"
     gene            complement(8587..9039)
                     /locus_tag="VEO00_08085"
     CDS             complement(8587..9039)
                     /locus_tag="VEO00_08085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU57993.1"
                     /translation="MEDAIDVRAVRAEETRGIRLAVLRPGRPRDELIFRGDDDPATFH
                     AGAFVAGTLVAVGRLSREAPKGEHDDAAWRVRSMATLPEHRNRGAGRAVLDALLEHAR
                     ERGASQVWCHARVRAVPFYERAGFGVEGDEFDLAGIGPHRFMRLRMPR"
     gene            complement(9095..9853)
                     /locus_tag="VEO00_08090"
     CDS             complement(9095..9853)
                     /locus_tag="VEO00_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HYU57994.1"
                     /translation="MGRPARPSHSPRRNPRAGIPSTCGAPGIAGPPPRFPATSGCAGK
                     RWSEEREVAEMRIGIIGTGNMARGFARGLSVKHDVILGSRDPAGARAVAEETGAAGVG
                     TYEEAARDADVVFLTVPWTAAIHVVEALPALAGKILVDVTNPYIDGELRPLEGSSTAE
                     EVQKRTSARVVKGWNTVLSKTLQDPSYGGIAPTVFLAGNDEIAKATVAELARDLGFVP
                     EDVGSVDETRMLERLQGLIGGLVWPEGAIRILHR"
     gene            complement(9816..>10095)
                     /locus_tag="VEO00_08095"
     CDS             complement(9816..>10095)
                     /locus_tag="VEO00_08095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003963241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU57995.1"
                     /translation="ITAVAIADDAPVAVGSHQPVGRITEITGVGTLPAYRRRGIGAAL
                     TSFLVQDALARGIETIFLTAGDEEIAGVYARTGFERVGTACAAEPLAA"
BASE COUNT         1433 a         3715 c         3602 g         1345 t
ORIGIN      
        1 cccggaccct gttcgacgcc ggggcgaagt actgctacgc gcaggggacc tcgatggccg
       61 gaccgcacgt ggtcggcgtg gcggcgctga tcgccagccg gtacgggccg ctcgcgccgg
      121 gggagctggc ggcgttcgtc gagcagtcgg cggagtccct ggcctgcccg gacccctacg
      181 agcctcaggg gaccgacttc ccgcggctgg acgggagccc acaggagtgc cagggcggca
      241 ccgggaacaa ctcgttctac ggcgccggcg aggtgaacgc ctacaacgcg gtcaccctct
      301 gacgctccgg cgcgccttcg gaggggccgg ttcgccggcc cctccggcac gttcggggcc
      361 gctagactcg cccgcgtgaa gaagctcgac ctcgacgaat cgttggtcca ggaggcgcgc
      421 gacctggccc gaagcatcgc ccggcccgtc gaggagtaca tcgccggaca caccaccgtg
      481 gcgatcgagc gcgccgtgct gcgcctgctc gggctcgatg gcgccgtggg cgaggggatg
      541 gagagccgtc cgttcccgaa cctcgtcgtc gacgcggtgc gcgagcagct cgggctgggg
      601 cgcggggtgg cgatcccggt attcgacgcg atggcgcggg agagcctctc gatccaccag
      661 gtcggcgccg cggtcgcggt cggcgagctg acgttccgcc tcccacccga cgagaccgag
      721 gtacgggaga cgatcgccgc cgccgcgcgg gaggcggacg ggcgcatcct ggctcgtcgc
      781 cgggagcgcg acgagctcgt ccaccacctc ggcgaccccg agaagccgtg gatctacctg
      841 atcgtcgcca ccgggaacat ccacgaggac atcccgcagg cgcaggccgc cgcccgtgcc
      901 ggcgccgacg tgatcgcggt gatccggtcg accgggcagt cgctgctcga ctacgtgccg
      961 tacggggcga ccatggaggg cttcgccggg acgttcgcga cgcaggagaa cttccggctg
     1021 atgcgccggg cgctcgacga gacatcgcag gagatcgggc gctacgtccg gctgacgaac
     1081 tacgccagcg gcctgtgcat gccggagatc gcggcgatgg ccgggctcga gcgcctggac
     1141 atgatgctga acgactgcat gtacgggatc atcttccgcg acatcaacat gcgccggacc
     1201 ttcgtggacc agcgcttctc gcggatgctc cacgcccgcg ccgggatcat catcaacacc
     1261 ggcgaggaca actacctgac cacacaggac cccgtggagg gagcgcgcgc cgtcgtcgcg
     1321 tcgcagctgc tgaacgagcg attcgcgctg gacgccggcc tggagccgtg gcagctcggg
     1381 ctgggccatg ccttcgagat cgaccccgac cggccggacg cctggctgtt cgagctggcg
     1441 cacgcccagc tggtgcgcga gctgttcccc gaggcgccgc tgaagtacat gcccccgacg
     1501 aagcacatga ccggcgacgt gttcatgggc tacctcctcg acggcatgtt ccacctgacg
     1561 ggcgtgctga ccgagcagga catcctgctg atcggcatga tgaccgaggg catccacacg
     1621 ccgttcctgt ccgaccgcga cctggcgatc cagaacgcgc gctacatggt gcgcacggcg
     1681 aggaacgtcg ccgcggagtg gtggccgccg cacgaggggc gtatccgggc ccgggcgcgg
     1741 caggtgctcg aggagacggt cgcccacctg cgccgggtgg acgacctcgg catgttcgag
     1801 gcgatccagg ccggcgagtt cgccgacacc agccgtccga tcgacggcgg ccacggcctt
     1861 gacggcgtgg tccgaaagga cgagggctac ctgaacccgt tcctcgagat ctgggaggcg
     1921 ccgtgaccga agccgacgtg cggcacgtcg tgcggccgta cggggacgac accgacgacg
     1981 gcaaggtgca ggtgtcgttc acgctgccga tcccgtcgga cgcgaaggcc gaggcggcgg
     2041 cccgggagct cgccgggaag atggggatgc gttccccgct cgtcgtgcac cggaaggcga
     2101 tcgggcgcct gttcacgttc ttcatcgtct acgggaccgt ggaccacgcg gtggacctgg
     2161 acgccatccg gatcgaggag cggccgttcc cgctgctcga ccacacggac gtgcggcgga
     2221 ggatccggca ggcgctcggt cgcgaggcgg tggtggtcgg cgccgccctg ggcaccgacg
     2281 cgcacaccgt cggtctcgac gcgatcctgt cgctgaaggg gatcgccggg gacaagggcc
     2341 tcgagtccta cgccgccttc cgcgtggtca acctccgctc tcaggtccgg ccggaggacc
     2401 tggtccgccg gtccctgcag gagggcgccg acgcagttct gatcagccag accgtcaccc
     2461 agaaggacgc gcacctccat cacctgcgcg agttcgccga cgaggtggac cgcgagggag
     2521 cccgcggccg gttcctgatg atcgccggcg ggccgcgcct gaccccggac atggccgccg
     2581 acctcgggta cgaccgggtg ttcggcaagg ggaccacccc gaccgaggtg gcgagctacc
     2641 tggcctgggc tctaccctcc gcagggggag gcgttcatga gcgatgagtt cccgcaggcg
     2701 gtgctgcgca tccggctctc gcagaccgac gcgcactacg gcggcaacct ggtgcccggg
     2761 tcgcggatcg tcgagctgtt cggcgacctg gccacggagc tgtgcgtccg gatggacggc
     2821 gacgagggcc tcttcgccgc gtacgaggag atctcgttca cggcgccggt ccacgccgga
     2881 gacttcctgg aggtgcgcgg gcggatcatc ggcgccgggc agaccagccg gcggatggcg
     2941 ttcgaggccc gcaagtacgt catcccccgg ccggagatct ccgagtcggc ggccgaggtg
     3001 ctcgaggagc cgctcgtcgt cgcccgggcc atcgggacct gcgtcgtgca gacgacccgc
     3061 cagcgcggca gggccgccac ccgtctggcc cgcgacgtcg agcacctctt cgacgagggc
     3121 taaggcgaac cttgccggag gacatccccg ggtacctggc ctccaaccgc gcgctgtggg
     3181 acgagtggac cgcgatccac gagacctcgt ccttctacga cctcgagggg ttcaaggccg
     3241 gcggcgtccg cctgcgcccg tacgaggtcg aggaggttgg cgacgtggcc ggcaaggacc
     3301 tgctgcacct gcagtgccac ttcgggatcg acacgatctc ctgggcccgg ctcggcgccc
     3361 gggtgaccgg cgccgacttc tccgagcgtg ggatcgcgct cgcgcgacgg gtggccgagg
     3421 aaatcgggat cgcggcacgg ttcgtccact cgaacgtgta cgacctcccc gacgctcccg
     3481 agctgcggga cgagcgcttc gacgtcgtct acacgtcgcg cggcgtgatc ggctggctcc
     3541 cggacctgtt cccgtgggcg cgcgtcgtgg cgcacttcct gaagcccggc ggcctcttct
     3601 acgttaccga gggccacccg acgatgtggg tgttcgacga cgccgaggac gtggagggcc
     3661 tgcaggtggc atacccctac tacgcgcggc cggagccgct ggcgttcgag gtgcagggct
     3721 cgtacgcgga gccgaccgcc gccgtgaaga gcgcgatgga gtactcgtgg ccgcactcga
     3781 tgggggagat cgtcacggcg atcgccgagg ccggcctggt gatcgagttc ctgcacgagt
     3841 tcccgttcct ggactggccg atgccgttcc tggaggacca gggcgacggc acctggcgcc
     3901 tgccggcctc ccaggcgggc gagatcccgc tgacgttctc cctgcgggcg cacaagccgt
     3961 gggagtgagc cgagaccggc ggcgacggcc ggtcaggacg ccagctgctc ggcgagggcg
     4021 gccgggtcgg tgaccggccg gcggcaggcg aatcgcgcgc agacgtaggc cgtcgcccgc
     4081 ccgtcgcgag cctcgcggcc ctcgaggagc ggggcgacct tcgccgcatc cggatcgccg
     4141 ggtcgcgcga ccgcgagcac cacgttcggc aggaaacgcc gccacacctc ggcggccagg
     4201 gcgtgggtgt cggccgccgc cggctcgccg accacggcga cctcgcgggc gggggagagg
     4261 tacaggtcga gggcctgcag cgcgtggccc gcggcgccgg aggcgcgctc catcagcggc
     4321 cgcgcgacgc gcagggccga gacggcggcc cgctcgtact ccgcctcccc gctcagcaga
     4381 tgcaggcgca gcagcacctc ggcggcgacc gagttcccgg acgggacggc gttgtcgaac
     4441 aggtccttcg gccgcacgac cagcgcctcg gcgtcggcgc cggtctggaa gaacccgccc
     4501 ccgtcccggt cgcggaacaa gcgcaccagc tcgtcggcca gggcacgcgc ctcggcgagc
     4561 cagcgcaggt cgaacgtcgt ctcgtgcagg accagcaggg ccgccgcgag gatggcgtgg
     4621 tcctcggcgt aggccggccc gctggtccgg ccgtcgcgcc aggcgcgcag caggcggccg
     4681 tcctcgcggc gcatccgctc caggacgaag cccgccgccg ccccggccgc ctccacgtac
     4741 cgcggctccc cgagcgcccg cccggcctcg gccagcgcct ggatcgccag gccgttccag
     4801 ccggccagca ccttgtcgtc cgtggccggc cggacgcgct cctcccgcgc cgcgtacagc
     4861 cgctcccgcg cggcgggcca gtccgccggc gaggggtcgc cgccggcccg ccacaggatg
     4921 ttcgtcccct cccggttccc gtcggggaga gcgccgagcc agcgagccac gtcctccccg
     4981 gccaccgcgc gcagctcgtc gtacggccag aggtagaact tcccctcgac gccctcggaa
     5041 tccgcgtcct gcgcggagta caggccgccc tccggcgccc gcatctcgcg caacaggtag
     5101 tcgagcgtct cgcggacgac cgccgcgtac caccccgtcc cggtgacctg ccaggcgtgc
     5161 aggtacagcc gcgcgagctg cgcgttgtcg tagagcatct tctcgaagtg gggcacgagc
     5221 cacatccggt cggtcgagta gcgcgcgaac ccgccgccga cctggtcgta catcccgccc
     5281 gaggccatcc gctccagcgt gcgcgtcgcc atgtccaggg cgccgggata cccgcggagg
     5341 tgcgtccgca gcagcagctc gaggttcatc ggctgcggga acttgggggc gggcccgaac
     5401 ccgccccact ccggatcgaa cgagcgctcc aggctgcggt acgccccccg caggaggtcc
     5461 tcggtcagcg gctcgcgcga ctcgtcgggc gtccgcgcga tcgccgccac cacgcgctcg
     5521 ccctgggcga ccacgcgctc gcggtcgtcg cgccacgcct cggcgagggc catcagcacc
     5581 cgcgagaggg ccggcatccc gtggcggtcc tcgttgggga agtaggtccc ggcgtagaac
     5641 ggggtgcccc cgggggtcag gaacaccgtc atcggccagc cgcccccgcc ggtcatcgcc
     5701 tgcacggcct ccatgtagat cgcgtcgacg tcgggccgct cctcacggtc caccttgatc
     5761 gagacgaagt gctcgttcat caggcgggcg gtcggggggt cctcgaacga ctcacgctcc
     5821 atgacgtggc accagtgaca ggcggcgtag cccaccgaca ggagcacggg cttgtcctcg
     5881 gcgcgcgcct tcgcgaacgc ctcctcgccc catgggtacc agtccacggg gttggcggcg
     5941 tgctggagga ggtacgggct ggtcgcgtcg gcgagacggt tcggcacgcc tccacgctag
     6001 cagggcaggt gagatgcccc gttgccgaag ggggtgggca gcctccctgg aaacgaccct
     6061 tgccgctgga aggggggcgg gtttaggctg acgccgttcc cgaccacagg gaggtggctc
     6121 gtgatggata cgccgctgat ggcgatgttg ccggcgtccg actacgaacg ggcgaaggcg
     6181 tggtaccgcg acaagctggg gctcaccccg gtccgcgatg agcccgaggg cgccgactac
     6241 gagatcggcg gggtcaggtt cggcgtctat cccagccagt tcgccggaac gaaccaggcc
     6301 acggccgctg ggttctacgt cgacgacatc gagaagacca tcgacgagct caccgccaag
     6361 ggagtggtgt tcgagcagta cgacatgccc gggctgaaga ccgacgagaa ggggatcgcc
     6421 gagttggggc ccttcaaggg cgcctggttc aaggacagcg aggggaacat cctggtggtg
     6481 tccgaggagg ccccgccctc ctaggcgctc ccgccgactc cccctgagtc gacggccgct
     6541 cgaagctcac cgaacagccg gaccaggtcc ggcacctggt aggcggccgt ccggccgctc
     6601 gggttgggca ggacccagac gggcgcgccg gccaggcgtt cgggctgggc accgaccgcg
     6661 gcgcgcggcc tgccgaacgc ggcgcggtag gccccgacgc ccaggaacgc caccgcggcc
     6721 gggcggtagc gcccgacgag ccgctcgacc ctccgcgccc ccgcgcggag ctcctgtcgc
     6781 gtgatctcgt ccgcgttccc gctcggccgc gccacgaggt tcgtgatccc gatcccccac
     6841 gccgggagtg acccgtcctc ggccgggccg agtacgcgat cggtgaaccc cgccccgtgc
     6901 agggccttcc agaaccggtt ccccggccgg gcgaagtggt ggccgacctc ggccgagcgc
     6961 agcccgggat tgatcccgac gaacaggacc cgcagccccg gctccaggac gtcggggacg
     7021 gtcgtcagtg cgtcggggcc gggttcggcg gctcgaacgg gccaacccac accatcgact
     7081 tggcgccggg ctggttgcag ggcggcgtgg agtcgatcgc cgggatcctc cgggcacctc
     7141 cccacgggag caccatgcgc caccggtccg gcgggttcat gaagcaccag ttcgcccagg
     7201 cgaaccgaac ccaggccctt ccgtccggca cgagcaccac gttgggccac cccgggggag
     7261 gcggttggcc gagcgaccgc cagtagggcg gcaccgagtt ctgttcgacc gggtaggcct
     7321 ccccgttcgc gttcacgggg atcagcctcg agcgaccctt cacggtgcac tcggaatagc
     7381 tggcattctt gacgacgatc tgaccgacca gcgacccggc ggcacccgtc aggtcgaacc
     7441 tccctcgcag catgtccggc gtacagaacg ggggaacccc ggcgtgtgcc gtgggggcgg
     7501 ctcccgccag cgtcgacacg gccagcagcg ctgccacgat ggccgccgat cggcggccgc
     7561 tccgaccacg catggctcgc acctccttcc gccgggacca gccccggctc cagggcgctc
     7621 gcagaacgga caataggaca ctttgctccg gcggtgagtt ccacagcccc ctaggctgtc
     7681 cctcgtgacg cgccgcgtgc tgctggacac ctccagcctg atgtaccggg cgttctttgc
     7741 gctgccgccg cagatccgcg cggccgacgg cacgccggcg aacgccgtcc acggctacct
     7801 gaactggacc gcagcgctga tccagtcgcg cgcgaccggc gacctgatcc actgctacga
     7861 ccacgactgg cgcccggcgt cgcgcgtggc cgagtacgac ggctacaagg cgacgcggca
     7921 ggtcgatccg ggaaacctgc cggatcagtt cgcctggctc cgcgaggttc tcgacgcgat
     7981 cggcatggca caggccgagg cggagggctg ggaggccgag gacgccatcg gctccatcgc
     8041 cgcccgcgcc cgcggtcgcg accggatcga catcgtcacc ggcgaccgcg acctcctcca
     8101 gctcgtgcgc gaccccgccg tcacgctctg gttcacccgc aagggcgtga cggagatgga
     8161 tcgctacgac gcggccgccg tgctggcgaa gtacggcatc ccgccctccc gctacgcgga
     8221 gttcgccatc ctgcgcggcg acccgagcga cgagctgccc ggcgtgaagg ggatcgggga
     8281 gaagacggcg cgcgagctgg tcaacgccta tcccgacctc gacgccctcc tggcggccgc
     8341 gcgatccgat ccccgcgcga agctcacccc gcggatccgg tcggccctga tcgacagcgc
     8401 agggtacgtg gcggcgatgc aacggatcgt cccgatccgc accgacctcg aggtgcgggt
     8461 gtggacgccg gtgccggacc cggaccgtgc cgaggagctc gccgatcgct accgggtggg
     8521 cggcccgctg cggcggatcc gcgaggcgct cgacgccgtc gggcgctgag tcgcgccccg
     8581 ccgcgctcag cgaggcatgc gcagccgcat gaagcggtgc gggccgatcc cggccaggtc
     8641 gaactcgtcg ccctcgacgc cgaacccggc ccgttcgtag aacgggaccg cccgaacccg
     8701 ggcgtggcac cacacctggc tcgcgccgcg ctcgcgcgcg tgctcgagca gtgcgtcgag
     8761 gacggcgcgg cccgcgccgc ggttgcggtg ctcggggagc gtggccatgc tccgcacgcg
     8821 ccaggcggcg tcatcgtgct cccccttcgg tgcttcacgc gagagcctcc cgacggccac
     8881 cagcgtcccg gccacgaacg ccccggcgtg gaacgtcgcc ggatcgtcgt cgcccctgaa
     8941 gatcagctcg tcgcggggac ggccggggcg cagcaccgcc aggcggatgc cacgggtctc
     9001 ctcggcccgc accgcccgga cgtcgatcgc atcctccacg accgcgaagg ctacccggac
     9061 gtccggggct cacgccggtg cgcgcgggcg gaggctaccg gtgcaggatg cggatcgcgc
     9121 cctccggcca caccaggccg ccgatcaggc cctgcagccg ctcgagcatc ctggtctcgt
     9181 ccaccgagcc cacgtcctcc ggcacgaacc ccaggtcgcg ggccagctcg gccaccgtgg
     9241 ccttggcgat ctcgtcgttg ccggccagga agacggtcgg cgcgatcccg ccgtaggacg
     9301 ggtcctgcag cgtcttggac agcacggtgt tccacccctt caccacgcgc gcgctggtgc
     9361 gcttctggac ctcttcagcc gtcgagctgc cctccagcgg ccgcagctcc ccgtcgatgt
     9421 aggggttcgt cacgtccacc aggatcttcc cggccagggc cgggagcgcc tcgacgacgt
     9481 ggatggccgc ggtccacgga acggtcagga agacgacgtc ggcgtcccga gcggcctcct
     9541 cgtaggtccc gaccccggcc gcgccggtct cctccgcgac cgcccgcgcc cccgccgggt
     9601 cgcgcgaccc gaggatcacg tcatgcttca cggagagccc gcgggcgaac ccccgcgcca
     9661 tgttcccggt cccgatgatc ccgatccgca tctcggccac ctcgcgctcc tcggaccaac
     9721 gctttcccgc gcaaccactc gtggcgggga accggggggg cgggccggct attcccggtg
     9781 ccccgcacgt ggacggtatc cccgctcggg ggttcctacg cggcgagtgg ctcggccgcg
     9841 caggccgtcc caacccgctc gaagccggtc cgggcgtaca cgccggcgat ctcctcgtcg
     9901 cccgcggtca ggaagatcgt ctcgatgccc cgcgccagcg cgtcctgcac caggaacgac
     9961 gtcagcgcgg caccgatccc gcggcgccgg taggccggca gcgtccccac gccggtgatc
    10021 tccgtgatcc ggcccacggg ctggtggctg ccgacggcga cgggggcgtc gtcggcgatc
    10081 gccaccgcgg tgatc
//
LOCUS       DASPWO010000352         5706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_52521, whole genome shotgun sequence.
ACCESSION   DASPWO010000352 DASPWO010000000
VERSION     DASPWO010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52521"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VEO00_08100"
     CDS             complement(<1..492)
                     /locus_tag="VEO00_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYU57996.1"
                     /translation="MDGKRPGRGRRAALAAVAALLLAACAGQAVDLGLSGAAAPTTAA
                     SPLPAVTTEDRVVQVVKRVEPAVVSVIADVVGAGGQDRAVGTGFIVRQDGVIVTNYHV
                     VETASRLTVVTTPPSPRRLEARVIGGDPDKDLAVLKVDAEGLATVPLGDSGGLQLGER
                     VVAI"
     gene            1010..2539
                     /locus_tag="VEO00_08105"
     CDS             1010..2539
                     /locus_tag="VEO00_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HYU57997.1"
                     /translation="MTDDRSEEHLHEEEHELLEEGGGAELHADAVPADVLATAREGLA
                     RISEVPKRVVVVGAGIAGLVAAFELKRQGHDVLLLEAQQRVGGRVHTCRNFAPGLYAE
                     FGAMRIPRVHDLTLAYCDLFGLELRPFVMGNPKALVYLNGERMTMAEADTHPERLPFE
                     LAEHERGRTWTDLWNEATREVIERYRREGPAALEAIVADFDQYSIREFLELRGFSEGA
                     IELYGVMSFRENNMNAAVIEQLREIVGRAFEDMQEITGGFDRLPNAFYAELKQNIRFG
                     ANVHALEQDERSVTVHYKTEAGRFSERADHVVLAVPFGILRDLETTPAFSREKQKAIR
                     ELNYNPSTKILFQTRTRFWEESDGIVGGTTTTDLPIRRICYPSHADPDEPRGTLLASY
                     TWGQDALRWGAMDEETRLEEAIEDVAKVHPEVREEYEIGATHSWYEDPWARGAFALFE
                     PEQESRLQADIVRPEGRIHFAGEHCSLWHAWIQGALESGIRAARAIHEAPAPSAASEP
                     R"
     gene            2536..3990
                     /locus_tag="VEO00_08110"
     CDS             2536..3990
                     /locus_tag="VEO00_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HYU57998.1"
                     /translation="MSDLPAPMTDAPLTRDVLTTPSEGLEPIPQTPKRVLVVGAGIAG
                     LVAAFELKRQGHEVLVLEAQNRVGGRIYTCRDFAPGLYAEFGAMRIPRAHDLTLAYCG
                     LFGLELRPFVMGNPAGLVYVAGRRMTMAEARANPDALPFELAEAERGRSPDDLWEQAI
                     GDYRRMVEEAGDQAWAEIVEELDRFSLYEFLKDRGFSEGAVEYWAVMNFVESDMNNGV
                     VEVLREDIGRAYEDMQEIAGGMDRLPNAFFRELQNDVRLGAEVHAIDQDERSVTVHFK
                     TEAGRFTATGDHAVCAIPFPVLRSIEMLTPLSREKQKAIRQLNYHASTKVLFQVRSRF
                     WEEEDGIFGGGTVTDLPVRRLNYPTPDPSTTRGVLLASYTWGQDALQWGAMDEETRLE
                     EALDDVSRIHPRIRDEYEVGASHAWYGDRWARGAFALFAPEQQTQLQEAIVRPEGRIH
                     FAGEHCSLYHAWIQGALESGIRAAREIHEAPAGP"
     gene            4050..4571
                     /locus_tag="VEO00_08115"
     CDS             4050..4571
                     /locus_tag="VEO00_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYU57999.1"
                     /translation="MGQDRYETLSAAVHALLGGGRSLEDVLGDVLRALGEGLGWDLAI
                     AWRADPSGSAMRFARAWRRPDGPGAALVARSETCVFSPGVGLPGRVLARREPAWLEDV
                     QVVPAFPRVAAAVEDGLHAGFGFPILDRHRVVGMIEFFCDEPRPPDGALLAMIDALGP
                     AIGALAAREWDAP"
     gene            complement(4600..5139)
                     /locus_tag="VEO00_08120"
     CDS             complement(4600..5139)
                     /locus_tag="VEO00_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58000.1"
                     /translation="MPRRTVLVSIALLAALGVLVGVVATTGSAEAPARRSWGRAWGIT
                     LGPDATASAASEVDQVLRVRLVTTNGAGVDVPPPGDSGGDYVVFTEDMLNQYGRKIGS
                     VVATCMLVFLHSRCDGTFFFDGHGTLEVAGALPPGVHGQVLPITGGTGDFENARGEGH
                     LSEGPNGATDFRFHIIIED"
     gene            5262..>5706
                     /locus_tag="VEO00_08125"
     CDS             5262..>5706
                     /locus_tag="VEO00_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU58001.1"
                     /translation="MLQVRDLTVKAGGRPVIEGASFSVRAGDKVALVGRNGAGKTSLL
                     QALAGEAPVAAGTVLRSDRVGYLRQEPLVRGGRDRAGDGGPTTVAYVLSGRGLDDAAE
                     RLERLRVGLEDRPTVRNIERYAGAEETFRAAGGYAAESEARRIAAG"
BASE COUNT          830 a         2013 c         2103 g          760 t
ORIGIN      
        1 gatggcgacg acccgctcgc cgagctgcag ccccccggag tcgccgaggg gcacggtggc
       61 cagcccctcg gcgtcgacct tcagcacagc gaggtccttg tccgggtcgc cgccgatgac
      121 ccgggcctcg aggcgccgcg gggaaggcgg cgtggtgacc accgtcaacc ggctggccgt
      181 ctcgaccacg tggtagttcg tgacgatcac gccgtcctgc cggacgatga accccgtccc
      241 gacggcgcgg tcctggcccc cggcgccgac gacgtccgcg atgacactga ccacggcggg
      301 ctctactcgc ttcaccacct ggaccacgcg gtcctcggtc gtgaccgccg gaagcgggga
      361 ggcggcggtg gtcggcgcgg ccgcgccgga gaggccgagg tccaccgcct ggccggcgca
      421 cgccgccagc agcagcgcgg ccaccgcggc cagcgcggct ctccggcccc gtcccggtcg
      481 ctttccgtcc atcatcagca ccctacctat catcggctcc tgagaaccgc ctgagatcgt
      541 ggaaaggaaa cgatgaaggc ggtctgcgag cgcctgatgg ctgacgcgcg ggcggctggc
      601 agccgggcgc ggtggcgctc ccctgcggcg agcccgtcaa gatcacccac ccagttcgct
      661 cggtgggttc ggcgctgggg gaacgagcat cgaccgcccg cggaagctgc tccacgggct
      721 tgacctggcc acggagagcg ggcgagcggg cggccgatgg tccctcaggg cgccgaggga
      781 cgtccgagcg atcgacccga gcataccccg acgtgagcgt tcgacggaaa ttacccgaga
      841 cggtcgatgt gtcccccatc cggggcgccc cacgcgctcc tccactctgc cccggccggc
      901 tgcatgccca acgtacgtcg aggtcgaggg accggatctt ccggcggtgc cccgcgcact
      961 agactcaccc gccgtcagcg gcgcggggac cggcggggag gaggcagcga tgaccgacga
     1021 ccgctccgag gagcacctcc acgaagagga gcacgagctg ctcgaggagg gcggcggcgc
     1081 ggagctgcac gccgatgccg tccccgcgga cgtgctggcc acggcgcgcg aggggctggc
     1141 acgcatctcc gaggtcccga agcgagtggt ggtcgtcggg gccgggatcg ccgggctggt
     1201 agcggccttc gagctgaagc ggcagggcca cgacgtgctg ctgctcgagg cacaacagcg
     1261 cgtcggcggc cgcgtccaca cgtgccggaa cttcgccccg ggcctgtacg cggagttcgg
     1321 cgcgatgcgc atcccccgcg tgcacgacct gacgctcgcc tactgcgacc tgttcgggct
     1381 cgagctgcgg ccgttcgtca tggggaaccc gaaggcgctc gtctatctga acggcgagcg
     1441 gatgaccatg gccgaggccg acacccaccc cgagcgcctc cccttcgagc tcgccgagca
     1501 cgagcgcggc cgaacctgga ccgacctgtg gaacgaggcc acgcgcgagg tgatcgagcg
     1561 ctaccggcgg gagggcccgg ccgcgctcga ggcgatcgtg gccgacttcg accagtactc
     1621 gatccgcgag ttcctggagc tgagggggtt ctccgagggc gccatcgagc tctacggggt
     1681 gatgtcgttc cgcgagaaca acatgaacgc cgcggtgatc gagcagctgc gcgagatcgt
     1741 cggccgggcg ttcgaggaca tgcaggagat caccggcggc ttcgaccgcc tgccgaatgc
     1801 cttctacgcc gagctgaagc agaacatccg cttcggggcg aacgtccacg ctctggagca
     1861 ggacgagcgc tcggtgaccg tgcactacaa gaccgaagcg ggccggttca gcgaacgcgc
     1921 cgatcacgtc gtgctggcgg ttccattcgg gatcctccgc gacctggaga cgaccccggc
     1981 gttctcgcgt gagaagcaga aggccatccg cgagctgaac tacaacccct cgacgaagat
     2041 cctgttccag acccggacgc ggttctggga ggagagcgac ggcatcgtcg gcgggacgac
     2101 taccaccgac ctcccgatcc gacgaatctg ctacccctcc cacgccgacc ccgacgagcc
     2161 gcgcgggacg ctgctcgcct cctacacctg gggccaggac gcgctgcgct ggggggcgat
     2221 ggacgaggag acgcgcctgg aggaggcgat cgaggacgtg gccaaggtcc atcccgaggt
     2281 tcgcgaggag tacgagatcg gcgccaccca ctcctggtac gaggacccct gggcgcgcgg
     2341 cgccttcgcg ctgttcgagc ccgagcagga gtcgcgactg caggcggaca tcgtccggcc
     2401 cgagggccgg atccatttcg ccggcgagca ctgctcgctg tggcacgcct ggatccaggg
     2461 cgcgctcgag tcggggatcc gggccgcgcg agccatccac gaggcgcccg caccttccgc
     2521 cgcctcggaa ccgcgatgag cgacctgccg gccccgatga cggatgcccc gctcactcgc
     2581 gacgtgctga cgacgccgtc cgaagggctg gagccgatcc cgcagacgcc gaagcgggtc
     2641 ctcgtcgtcg gcgccggcat cgccgggctg gtcgcggcct tcgagctgaa gcgacagggc
     2701 cacgaggtgc tggtgctcga ggcccagaac cgcgtcggcg ggcgaatcta cacctgccga
     2761 gacttcgccc cggggctgta cgcggagttc ggggcgatgc gcatcccgcg ggcgcacgac
     2821 ctgacgctcg cctactgcgg cctgttcggc ctggagctgc ggcccttcgt gatgggcaac
     2881 ccggccggcc tggtctacgt cgccgggcgg cggatgacga tggctgaagc ccgggcgaac
     2941 ccggacgcgc tgccgttcga gctggcggag gccgagcgcg gccggagccc ggacgacctg
     3001 tgggagcagg cgatcggcga ctaccgccgg atggtcgagg aggccggtga ccaggcgtgg
     3061 gccgagatcg tcgaggagct cgaccggttc tcgctgtacg agttcctgaa ggaccgcggg
     3121 ttctccgagg gcgccgtcga gtactgggcg gtgatgaact tcgtcgagtc cgacatgaac
     3181 aacggggtgg tcgaggtgct gcgcgaggac atcggccggg cctacgagga catgcaggag
     3241 atcgccggcg ggatggaccg gctgccgaac gccttcttcc gagagctgca gaacgatgtg
     3301 cgcctcggcg ccgaggtgca tgccatcgac caggacgaac ggtcggtgac cgtgcacttc
     3361 aagaccgagg cggggcggtt caccgccacc ggcgaccacg ccgtctgcgc gatcccgttc
     3421 ccggtgctgc gctcgatcga gatgctcacg ccgctgtcgc gcgagaagca gaaggcgata
     3481 cggcagctga actaccacgc ctcgacgaag gtgctgttcc aggtgcgcag ccggttctgg
     3541 gaggaggagg acggcatctt cggcggcggg acggtcaccg acctgcccgt tcgccgcctg
     3601 aactacccga cgcccgaccc ctccaccacg cgcggcgtgc tgctcgcgtc ctacacctgg
     3661 ggccaggacg ccctgcagtg gggggcgatg gacgaagaga cgcggctgga ggaggcgctc
     3721 gacgacgtgt cccggatcca cccgcggatc cgcgacgagt acgaggtcgg cgcgtcgcac
     3781 gcgtggtacg gggaccgctg ggcgcgcggg gcgttcgcgt tgttcgcgcc cgagcagcag
     3841 acccagctcc aggaggcgat cgtccggccc gagggccgga tccacttcgc cggcgagcat
     3901 tgctccctgt accacgcctg gatccaggga gcgctcgagt ccgggatccg cgccgctcgg
     3961 gagatccacg aggcacccgc cgggccgtga actccgcgcc gcgccctcgg taggatcgag
     4021 ggaggcgagg cgagcggacg gagaccggcg tgggccagga tcgctacgag accctttccg
     4081 ccgccgtgca cgcgctcctc ggtggagggc ggtccctcga ggacgtcctc ggcgacgtgc
     4141 tacgcgccct cggcgagggg ctgggctggg acctcgcgat cgcctggcgg gcggatccct
     4201 ccggctccgc gatgcgattc gcccgcgctt ggcggaggcc ggacggccct ggggcggcgc
     4261 tcgtcgcgcg cagcgagacg tgcgtcttct cccccggcgt cggcctcccg gggcgggtcc
     4321 tggcccggcg cgagcccgcg tggctggagg acgtccaggt cgtgccggcc ttcccccgcg
     4381 tggccgccgc ggtcgaggac ggtctccacg cggggttcgg cttcccgatc ctcgaccgcc
     4441 accgggtcgt cggcatgatc gagttcttct gcgacgagcc ccgcccgccg gacggagcgc
     4501 tcctggcaat gatcgatgcc ctcggtccgg cgatcggggc gctcgccgcc cgcgaatggg
     4561 acgcgccctg acgctccccg cccggctccg gccggtcgct cagtcctcga tgatgatgtg
     4621 gaaccggaag tccgtcgcgc cattcgggcc ctcggacagg tggccctcgc cgcgggcgtt
     4681 ctcgaagtcc cccgtgcccc ccgtgatcgg cagcacttgc ccgtgcaccc ccggcggcag
     4741 cgcgccggcg acctcgagcg tgccgtgtcc gtcgaagaag aacgtgccgt cgcagcgcga
     4801 gtggaggaac accagcatgc aggtcgcgac cacggagccg atcttccggc catactgatt
     4861 gagcatgtcc tcggtgaaga cgacgtagtc gccgccactg tcgcccggtg gcgggacgtc
     4921 cacgccggct ccgttcgtgg tcaccaggcg cacgcggagc acctggtcca cctcggacgc
     4981 cgccgaggct gtcgcgtccg ggccgagggt gatgccccac gcgcgacccc acgagcgacg
     5041 cgcgggggcc tcggccgaac ccgtggtggc gaccacgccg accagcacgc cgagggcggc
     5101 gaggagggcg atcgatacaa ggacggtgcg tcttggcacg gccttctcct ttcgaggggc
     5161 tgcgggtgtg gcgcgatcct acgggccgcc ccccgcccgg taaaggaccc gccggcggtg
     5221 ccgttgccgg gcgagccccc cgctcgcata ctcggcggga catgctgcag gttcgcgacc
     5281 tgacggtgaa ggcgggcggg cgccccgtga tcgagggcgc ctcgttctcg gtgcgggccg
     5341 gcgacaaggt cgccctggtg gggcgcaacg gcgcgggcaa gaccagcctg ctgcaggcac
     5401 tcgccggaga ggcgccggtc gccgcgggaa ccgtgctccg ttccgaccgg gtcggctacc
     5461 tgcgccagga accgctggtc cggggcggac gcgatcgggc cggcgacggg ggcccgacca
     5521 cggtggcgta cgtgctctcc ggccgcgggc tggacgacgc ggcggagcgg ctggagcgcc
     5581 tgcgcgtggg cctcgaggat cgcccgacgg tacggaacat cgagcggtac gcgggcgcgg
     5641 aggagacgtt ccgggcggcc ggcggctacg cggccgagtc cgaggcgcgc cggatcgcgg
     5701 cgggcc
//
LOCUS       DASPWO010000353         5401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_393360, whole genome shotgun sequence.
ACCESSION   DASPWO010000353 DASPWO010000000
VERSION     DASPWO010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393360"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VEO00_08130"
     CDS             <1..607
                     /locus_tag="VEO00_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58002.1"
                     /translation="DPVPAPIPIAFDPSTFLDDAQDELPADEPVLAPDADATPPAPDG
                     LGDDRAVLTDAELEELSERGIAGPLAGMSLDDLQLDDVSIDDPVLSPDAAVTMPAGAL
                     GDPDPFQPDFPTEYVAPVDYTLDADTSAIELDDGVEPIDMDPVVDVDVDPVPIDGPVE
                     PIILPEPYPEEPIQPQDEPIDMEPAVEYDPIGPDPAVDPGP"
     gene            complement(644..1114)
                     /locus_tag="VEO00_08135"
     CDS             complement(644..1114)
                     /locus_tag="VEO00_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58003.1"
                     /translation="MRRWGVVIVMLAAAAVPIGVLAATGTGSAQVNRQRFAYTTDGFT
                     TTSTTFVDVPGLVVNVCSDNLVAANVGLGLGGAQVSVRAVADDGPVMFPGPVIFAPGS
                     TTGFSFPFARALSAFEDDDSHQIKVQVRSTTGASVNVNRGTLIVQYQQGDQGCP"
     gene            1331..2719
                     /locus_tag="VEO00_08140"
     CDS             1331..2719
                     /locus_tag="VEO00_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58004.1"
                     /translation="MGGRRRAVVGMAGALLVVAVAAGSGPGLAAARVRAGARAAAGVR
                     GALAATTLQIFHGSPLLVRAGEPVIVPVDVVCVTASGMACPATVSIRAGAGLARRAEG
                     PIRQGLRFDLTGLVAGKPAGAGEGAGPAGPVAGSVPFAIAARDPAGRTVTLPAVGALR
                     VYVAAAMRAVAVPAVPFGRLRDGTTVLALPWGRGAAAAGLDRGPEQATVGPPAFDVGA
                     NGRVYLLDALQGRLAVFGGGRLVRQTALDVAPRSDLSVAPDGTAWVLTRAVASGLVRL
                     VRVDPAGRPGEEVAAGDGPLGQVRAVGGRAYARLLPMDAWVAAGPGVAAAAEPSVGRP
                     LGDGTALLRVAGERTVRLGIARGERVEDAVELRFASPVGEVALAEPDGRGGYVMVARV
                     YREGANAADQFQVVLVSASGAVRTFAVSSAAYAEPAPSGRFRLGPDGALYRMTSSPEG
                     VRIVRHDLGGTR"
     gene            2716..3375
                     /locus_tag="VEO00_08145"
     CDS             2716..3375
                     /locus_tag="VEO00_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58005.1"
                     /translation="MKRLLVAMALSAAAVVAVPGTALASWVDDKCFVDAHSVDMLERS
                     QARAYAESGIDEGYEYAGGCWDNDDRDDTPGQPDSEGEGPDCSGFVFKTWHLRGIIGN
                     DGFRWYNRLQDVHGPYSSYTFHDGDPDYPFRTLASKSNALYMDAFAKHGHIGMLYTGN
                     GPSDGTDYILEAWCDACGTGVNVQTYRGNADYVGVRRRDWTPDCYPQCQGPAEVTLIR
                     L"
     gene            3416..4129
                     /locus_tag="VEO00_08150"
     CDS             3416..4129
                     /locus_tag="VEO00_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58006.1"
                     /translation="MRGARWVVPAILLALVPAACSGSGPPTPSPSASPATGTPDGTTG
                     PGPGDDRQAFAVWPEDVRSSAEATADRVAAGELGWRRRPDAMAGMFAGRVLGWPGAGV
                     EPFEDDGCDGCYLVTRADGGGAVFVRSEPLVGEIWSVVSVQAVEDGEIEDYRLAFSVS
                     GGRARGAIDLGDEPGATLVLAYGERSAEVDVAPPGEFDLDLGFEPPTSGHFLVLFGED
                     GAVSTAVAGTLPAGDFAAS"
     gene            4161..4769
                     /locus_tag="VEO00_08155"
     CDS             4161..4769
                     /locus_tag="VEO00_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HYU58007.1"
                     /translation="MAFVRRHPVFLGLVVLLALVVVLVLGTGVAVWQAAHRDEASRID
                     RADLVIVLGAAQYNGTPSPVFRARLDHAARMYRDGFSELILVVGGGQPGDVTTEGEAG
                     RGYLVEDQVIPPEDVFASSEGRSTIESLRGARAFMDDRGLRSAFLVSDPWHNLRLLRM
                     AGDLGIDGHVSATSSAARTQQTRLAGYVRETFAYLWYRLTGR"
     gene            4813..>5401
                     /locus_tag="VEO00_08160"
     CDS             4813..>5401
                     /locus_tag="VEO00_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58008.1"
                     /translation="MGAFSGFSWSNRATKWLWFTAGLELLLAVIFLNVGAFGPSEAKG
                     GMYLTAAILGGVGILLVLWARSATKNYERAQALRQSGLAGTARVLSAEFTGTTMNEVP
                     YMRMDLEVTIPGRPPYQVEHKDYVPIGRQFMLGPHATIPVKVNPNDPTDLVIEWEGAS
                     INPVVVTTPFESQPAAGAGGFGALAGAMLLGAAAAA"
BASE COUNT          640 a         1927 c         2103 g          731 t
ORIGIN      
        1 cgaccccgtc ccggcgccga tcccgatcgc cttcgacccc tcgacgttcc tcgacgacgc
       61 gcaggacgag ctgccggccg acgagccggt cctggcgccc gatgcggacg cgacgccgcc
      121 cgcgcccgac gggctgggcg acgatcgggc cgtgctgacc gacgccgagc tggaggagct
      181 ctcggagcgc gggatcgccg ggccgctcgc cggcatgtcg ctcgacgacc tgcagctcga
      241 cgacgtgtcg atcgacgacc ccgtgctgtc gccggacgcg gcggtgacga tgccggccgg
      301 ggcgctcggg gaccccgatc ccttccagcc ggacttcccg accgagtacg tggcgccggt
      361 cgactacacg ctcgacgccg acaccagcgc gatcgagctc gacgacggcg tcgagccgat
      421 cgacatggac ccggtggtgg acgtcgacgt cgacccggtg ccgatcgacg gcccggtgga
      481 gccgatcatc ctcccggagc cgtacccgga ggagccgatc cagccgcagg acgagccgat
      541 cgacatggag cccgccgtcg agtacgaccc gatcggcccc gaccccgcgg tcgaccccgg
      601 cccgtaaggc cgggctccct ccccggccag cggcgcgccc gctctacggg cagccctggt
      661 cgccctgctg gtactgcacg atcagcgtgc cccggttgac gttcaccgac gcccccgtgg
      721 tggagcggac ctggaccttg atctggtggc tgtcgtcgtc ctcgaaggcg gagagcgccc
      781 gggcgaaggg gaacgagaac ccggtggtcg agccgggcgc gaagatcacc ggtcccggga
      841 acatcaccgg cccgtcgtcg gccaccgctc gcacggatac ctgcgcgccc ccgaggccga
      901 ggccgacgtt cgcggccacc aggttgtccg agcagacgtt gacgaccagc cccggcacgt
      961 cgacgaacgt cgtgctcgtc gtggtgaacc cgtcggtcgt gtaggcgaac cgctgccggt
     1021 tgacctgcgc cgagccggtc ccggtggccg ccaggacccc gatcggaacg gctgcggcag
     1081 ccagcatgac gatcacgacg ccccacctgc gcatggcacg ctcccttcgt cggtttgggc
     1141 acttctacca ccgctcaccc ccacgcgacg gacgtccggt ggctcgatcc ggttccggat
     1201 cccccgatcg gggcgcggaa acccccggcc gggggatgga gcggccgatc cgtccccgta
     1261 tcgtcccgaa cgtcggttcg atccgttcga ggtcacctcc ctcgctggcg aacgggaggg
     1321 aggtgatggg atgggcggaa ggcgacgagc ggtcgtcggg atggcgggtg cgctcctcgt
     1381 ggtcgcggtg gcggcgggtt ccgggcccgg gctcgcggcg gcgcgggtcc gggcgggggc
     1441 ccgggccgcg gccggggtgc ggggggcgct cgcggcgacg accctccaga tcttccacgg
     1501 ctcgcccctc ctcgttcggg cgggggagcc ggtgatcgtc ccggtggacg tggtatgcgt
     1561 cacggcctcg gggatggcct gcccggcgac ggtgtcgatc cgggcgggag cggggctcgc
     1621 gcggcgagcg gaggggccaa tccggcaggg actgcgcttc gacctcacgg ggctcgtggc
     1681 ggggaagccc gccggggccg gggaaggcgc agggcccgcc gggcccgtgg ccgggtccgt
     1741 gcccttcgcg atcgccgcgc gagatcccgc cgggcggacg gtgacgctcc cggcggtcgg
     1801 ggcgctgcgc gtctacgtgg ccgcggcgat gcgcgcggtg gcggtgccgg cggtgccgtt
     1861 cggccggctc cgcgacggga ccacggtcct ggccctgccg tggggccgcg gcgcggcggc
     1921 cgccgggctc gaccgcgggc cggagcaggc caccgtcggg ccgccggcgt tcgacgtcgg
     1981 tgcgaacggg cgcgtgtacc tgctcgacgc cctgcagggg cggctggcgg tcttcggcgg
     2041 cggccggctg gtgcgccaga cggccctgga cgtcgcgccg cgctcggacc tctcggtggc
     2101 gcccgacggg acggcgtggg tcctgacccg tgccgtggcg agcgggctgg tccggctcgt
     2161 ccgggtcgac ccggccgggc ggcccggcga ggaggtggcg gccggagacg gtccgctcgg
     2221 gcaggtccgg gcggtgggtg gccgcgcgta cgcgcgtctg ctgccgatgg acgcctgggt
     2281 cgcagccggg cctggggtcg cggctgcggc cgagccgtcg gtcgggcggc cgctcggcga
     2341 cgggacggcg ctgctgcgcg tggccggcga gcggacggtg cggctcggca tcgcccgcgg
     2401 cgagcgggtc gaggacgcgg tcgagctgcg gttcgcgtcc ccggtgggcg aggtggcgct
     2461 cgccgagccg gatggccgcg gcggctacgt gatggtcgcg cgcgtgtacc gtgagggcgc
     2521 gaacgccgcc gaccagttcc aggtggtgct ggtgtcggcg tcgggagccg tgcggacgtt
     2581 cgcggtgtcg tcggcggcgt acgccgagcc ggcgccgtcc gggcggttcc gcctgggccc
     2641 cgacggcgcc ctgtaccgga tgacgtcctc cccggagggg gtgcgcatcg tgcgccacga
     2701 tctgggcggg acgcgatgaa gcggctgctg gtggcgatgg cgctctcggc cgccgcggtg
     2761 gtggccgtgc cgggcacggc cctggcgtcc tgggtcgacg acaagtgctt cgtcgacgcc
     2821 cactcggtcg acatgctcga acggagccag gcccgtgcct acgccgagtc ggggatcgac
     2881 gagggctacg agtacgccgg cgggtgctgg gacaacgacg accgggacga cacgcctggc
     2941 cagccagact ccgagggcga gggcccggac tgctcgggct tcgtgttcaa gacctggcac
     3001 ctgcgcggga tcatcggcaa cgacgggttc cgctggtaca accgactcca ggacgtccac
     3061 gggccgtact cgtcgtacac gttccacgac ggcgaccccg actacccgtt ccggaccctc
     3121 gccagcaagt ccaacgcgct gtacatggac gcgttcgcga agcacggcca catcgggatg
     3181 ctctacacgg ggaacggtcc gtccgacggg accgactaca tcctcgaggc ctggtgcgac
     3241 gcgtgtggga ccggggtgaa cgtgcagacc taccgcggca acgcggacta cgtcggcgtc
     3301 cggcgcaggg actggacgcc ggactgctac ccgcagtgcc aggggcccgc ggaggtgacg
     3361 ctgatccggc tgtagcgggt gtgacggccg cgtgagcgcg gcgattggag gcgcgatgcg
     3421 aggcgcgagg tgggtggtcc cggcgatcct gctggccctg gtcccggcag cctgctcggg
     3481 gtccgggccg cccaccccga gcccgtcggc gagcccggcg accggtacgc cggacgggac
     3541 caccggcccc ggcccggggg acgaccgcca ggccttcgcg gtctggccgg aggacgtgcg
     3601 ctcgtccgcc gaggccacgg cggaccgggt ggcggcgggg gagctcggct ggcggaggcg
     3661 gccggacgcc atggccggca tgttcgccgg gagggtcctc ggctggccgg gcgcgggcgt
     3721 cgagccgttc gaggacgacg gctgcgacgg gtgttacctg gtcacccgcg ccgacggcgg
     3781 aggcgcggtc ttcgtccgtt cggaaccgct ggtcggcgag atctggtcgg tggtcagcgt
     3841 gcaggccgtc gaggacggcg agatcgagga ctaccggctg gccttctccg tctccggcgg
     3901 ccgggcccgg ggggcgatcg acctcgggga cgagccgggg gccaccctcg tgctggccta
     3961 cggcgagcgc tcggccgagg tcgacgtggc gccgccgggg gagttcgacc tcgatctggg
     4021 gttcgagccc ccgacgagcg gccacttcct ggtgctgttc ggcgaggacg gagcggtgtc
     4081 gacggcggtc gccgggacgc tgccggcagg ggacttcgcg gcgtcctgag cactgccggg
     4141 gccggtgcta gcctccgccc gtggcgttcg ttcgccggca cccggtcttc ctggggctgg
     4201 tggtgctcct cgcccttgtc gtcgtcctcg tcctcgggac gggcgtcgcc gtctggcagg
     4261 cggcccaccg cgacgaggcg agccgcatcg accgcgccga cctggtgatc gtcctcggcg
     4321 cggcccagta caacggcacg ccctcgccgg tgttccgggc ccgcctcgac cacgccgcgc
     4381 ggatgtaccg ggacgggttc tccgagctga tcctggtcgt cggcggcggc cagcccggcg
     4441 acgtgacgac ggagggcgag gccgggcgcg gctacctggt cgaggaccag gtgatcccgc
     4501 cggaggacgt cttcgcctcg agcgaggggc gcagcacgat cgagtccctg cgcggcgcgc
     4561 gcgcgttcat ggacgaccgc ggcctgcgct cggcgttcct ggtctccgac ccctggcaca
     4621 acctgcgcct gctgcggatg gcgggcgacc tcggcatcga cggccacgtc tccgccacct
     4681 cgtcggccgc ccgcacccag cagacccgcc tggccggcta cgtccgcgag accttcgcct
     4741 acctctggta ccgcctcacc ggccgctgac cctcgcttga caagccggcg gggcgtccgg
     4801 acgatgcccg ccatgggtgc attcagcggg ttctcgtggt ccaaccgggc caccaagtgg
     4861 ctgtggttca cggcggggct cgagttgctg ctggcggtga tcttcctgaa cgtcggcgcg
     4921 ttcggcccgt ccgaggcgaa gggcggcatg tacctgaccg cggccatcct gggcggcgtc
     4981 gggatcctgc tggtgctctg ggcgcgctcc gccaccaaga actacgagcg ggcccaggcg
     5041 ctgcgccaga gcggcctcgc cgggacggcc cgggtcctct ccgccgagtt caccgggacg
     5101 accatgaacg aggtcccgta catgcggatg gacctcgagg tgacgatccc cgggcgcccg
     5161 ccgtaccagg tcgagcacaa ggactacgtg ccgatcgggc ggcagttcat gctcggcccg
     5221 cacgccacga tcccggtgaa ggtcaacccg aacgacccga ccgacctggt gatcgagtgg
     5281 gagggcgcca gcatcaaccc ggtcgtcgtc accacgccgt tcgagtccca gcccgccgcc
     5341 ggggccggag gcttcggcgc gctcgccggg gcgatgctgc tcggcgccgc ggcggccgcg
     5401 g
//
LOCUS       DASPWO010000354         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1073255, whole genome shotgun sequence.
ACCESSION   DASPWO010000354 DASPWO010000000
VERSION     DASPWO010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073255"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VEO00_08165"
     CDS             <1..338
                     /locus_tag="VEO00_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58009.1"
                     /translation="VERGLMVFFAALGRDLALEADRQLAGGAAAAPTGSRFLEAIWRY
                     YNLVDFAVSRRDAGASVAMRLAYDDAEALVRPAATPAAVSSARVRESFRRIADILSGI
                     IQAPPAPGR"
     gene            complement(307..>2240)
                     /locus_tag="VEO00_08170"
     CDS             complement(307..>2240)
                     /locus_tag="VEO00_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYU58010.1"
                     /translation="PLVHDPARGNVFLSRRFESGDVESALAGAAVVVEQTFRTNRQCA
                     APLEGRGGVADWSDPEGRLTLWSGTQVPHLARHLLAEILGLPENKVRVVAPDVGGGFG
                     VKAILYPEDIALCVLARTLGRPVKWVEQRREAMQASAHARDHHYTVRAGFDARGHLLG
                     LDVRAVCNAGAYSVFPWTAGIEALMAGGLLTGPYKLQHYRCQVTAVATHTTPAGPYRG
                     VARPATTFVMERVLDLGARRLGLDPVDIRRVNLVGPADLPYTSPTRLVHDSPSYPVCF
                     KKVVEAIDYGSFRAEQARLRTAGRYVGVGFAVYNELTGLGQAASAGPRMPFRTGHEGA
                     TVRLDPSGAVTVLAGVTSQGQGLETTVAQIVAAELAVPLDTVRVVLGDTDATPFGLGA
                     FASRQAVIGGGATMRAARAVRDKALAIAAHVLEAAVEDLDAADGQVFVRGAPGRALAM
                     SEIARIAHLETHRLPPDVDPGLDATRFYDPIRGTFAAGAQAAIVEVEPETGVIAIRRY
                     VCVEDTGRIINPLIVEGQVHGAIAQGIGGALSEHLVYDETGQLLTGTFMEYAIPTAAV
                     IPPLETEHVEEPAANLAGVRGVGEGGTLGPAAVLANAVADALAPIGVEPDELPLTPAR
                     LWTACARAGLSARAPAAPG"
BASE COUNT          305 a          814 c          822 g          299 t
ORIGIN      
        1 aggtcgagcg tggcctgatg gtcttcttcg ccgctctcgg ccgcgacctg gccctggagg
       61 ccgaccgcca gctcgccggg ggagctgccg ccgccccgac cggctcccgg tttctcgagg
      121 cgatctggcg ctactacaac ctcgtcgact tcgcggtcag ccgccgcgac gccggcgcgt
      181 cggtggccat gcgcctggcc tacgacgacg ccgaggcgct cgtgcgtccg gccgcgacgc
      241 cggccgccgt cagctcggcg cgtgtgcgcg aatcgttccg gcgcatcgcg gacatcctgt
      301 cgggaatcat ccaggcgccg ccggcgcccg ggcgctgagg ccggcgcgcg cacacgcggt
      361 ccagaggcgg gcgggcgtga gcggcagctc gtccggttcc acgccgattg gagcgagcgc
      421 gtcggcgacg gcgttcgcga ggacggccgc cggaccgagc gtgccaccct cgccgacccc
      481 acggacaccc gccaggttgg cggcgggctc ctcgacgtgc tcggtctcga ggggcgggat
      541 gacggcggcc gtcgggattg cgtactccat gaaggtgccg gtgaggagct ggcccgtctc
      601 gtcgtacacc aggtgctccg acagcgcgcc cccgatcccc tgcgcgatcg ccccgtgcac
      661 ctggccctcg acgatcagcg ggttgatgat gcgcccggtg tcctccacgc acacgtaccg
      721 ccggatcgcg ataacgcccg tctcgggctc gacctcgacg atggcggctt gggcgccggc
      781 cgcaaacgtc ccgcggatcg gatcgtagaa ccgggtggcg tcgaggccgg gatcgacgtc
      841 ggggggcagc cggtgagtct ccaggtgcgc gatccgcgcg atctccgaca tcgcgagcgc
      901 gcgcccgggt gcgccccgca cgaacacctg gccgtcggcg gcgtcgaggt cctcgaccgc
      961 cgcctccagc acatgcgcgg ctatcgcgag cgccttgtcg cgcacagccc gggctgcgcg
     1021 catcgtggcg ccgccgccga tgacggcctg acgcgaggcg aaggcgccga ggccgaacgg
     1081 cgtggcgtcg gtgtcgccca gcacgacgcg cacggtatcg agcggcaccg ccagctcggc
     1141 ggcgacgatc tgcgccaccg ttgtctccag cccctgcccc tgcgaggtga cgccggccag
     1201 cacggtgacg gcgcccgagg gatccaggcg cacggtggcg ccctcgtggc cggtccggaa
     1261 cggcatgcgc gggccggccg acgccgcctg ccccagcccg gtcagctcgt tgtagacggc
     1321 gaagccgacg ccgacgtagc gcccggccgt gcggagtcgg gcttgctccg cgcggaagga
     1381 cccatagtcg atcgcctcga cgaccttctt gaagcagacg ggatagctcg ggctgtcgtg
     1441 cacgagtcgc gtcggcgagg tgtacggcag gtcggcgggg ccgacgagat tcacacgacg
     1501 aatgtcgacc gggtcgagac caagtcgccg ggcgccgagg tcgagcacgc gctccatcac
     1561 gaaggtcgtc gccggccgcg ccactccgcg atacggcccg gccggagtcg tgtgcgtggc
     1621 cacggcggtc acctggcagc ggtagtgctg gagcttgtag ggtccggtca gcaggccgcc
     1681 agccatcagc gcctcgatgc cagccgtcca cgggaagacg gaatacgccc ccgcattgca
     1741 cacggcgcgg acgtcgaggc ccagcaggtg gccgcgcgca tcgaatcctg cccggaccgt
     1801 gtagtggtga tcgcgcgcgt gggccgaggc ctgcatcgcc tcccgccgtt gctccaccca
     1861 cttgaccggc cgcccgagcg ttcgggcgag cacgcagagg gcgatgtcct caggatagag
     1921 gatcgccttc acgccgaagc cgccgccgac gtcgggggca acgacccgca ccttgttctc
     1981 cggcaggccg aggatctcgg ccaagaggtg gcgggcgagg tgggggacct gcgttcccga
     2041 ccagagcgtg agccgaccct ccgggtcgct ccagtccgcg acgccgccgc ggccttccag
     2101 cggtgccgca cactggcggt tcgtgcgaaa ggtctgctcg acgacgacgg cggcgccggc
     2161 cagcgccgat tccacgtcgc cgctctcgaa acgccgggag aggaagacgt tgccgcgtgc
     2221 ggggtcgtgg acgagcggcg
//
LOCUS       DASPWO010000355         4956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_474412, whole genome shotgun sequence.
ACCESSION   DASPWO010000355 DASPWO010000000
VERSION     DASPWO010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474412"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1371)
                     /locus_tag="VEO00_08175"
     CDS             complement(169..1371)
                     /locus_tag="VEO00_08175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYU58011.1"
                     /translation="MPIAYRDDPPVSAEVVIVGGGIVGAATAFHAARAGLRPLVLERR
                     PALSTLTTAAATGGFRLQLDDDEEVALVRESVDLFRHFADATGQREHDPAVREAGYLW
                     LTTSEATAAAQRETVERQRGWGLDDVDLVTGEDVRLSWPFVAPEVVQARFRQADGLLD
                     PIQAALGLAAGASALGAAFAPGVEVTGFRLVGDRVDAVETSRGVVRTAAAVIAAGPFS
                     GALAARAGARLPLATIRRQKVVVPQAPEVPADAPMVIDEDTGAHWRPALGGAFVLGPD
                     PGERPSPPTDPVPTDPALAFRLLDPRNDASVARVAPFWNDVWERSPQFFMAAGHYTMT
                     PDHRPLLGPAGIEGLWVNTGYSGRGVMGGPAGSRHLADVLTGRIPPSDNAYRPDRPMP
                     DRRRTEAL"
     gene            complement(1395..3569)
                     /locus_tag="VEO00_08180"
     CDS             complement(1395..3569)
                     /locus_tag="VEO00_08180"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYU58012.1"
                     /translation="MVERLGRWCYAHRWRVLVLWIVALVAFIALSNVAGGEYKADFNL
                     PGTESQEVFDLLEERFPQGAGDQAVLVFKATDGVADPLVRRDMEALFADIAELPRVLS
                     VASPYETPEGAAPQISPDGRIGFAAVQYEVPATEMKAEEVERLRDLGEAAQRARLQIE
                     FGGPVIEFAEFEPPGGAELVGVLAAMIILLLTFGSVLAMGLPIMTALFGIGIGIALVL
                     LSANVLSVPNFTTQLVSMIGIGVGIDYALFIVTRYRQGLSEGKEPEEAVAKSMSTSGR
                     AVLFAGSVVVISFLGILLLRFSFIEGIAVGGAAAVLVTMLASLTLLPAVLGFVGRNID
                     RFHIPRLRRAEGAGRRSFWYRWSRLVQRRPWTALLIGLLIMGTLTVPLFRMRLGFADA
                     GNNPESYTSRQAYDLLSEGFGPGFNGALVLVAELHGPEDLSVLQDLAAAAGETEGVAA
                     VSPPIPTPEGDAALIRLFPTTAPQDQATNDLVRRLRGEVVPAVTEGTGVDVKVGGQTA
                     AIVDFSRLIADRLPLLIAVVIALSFLLLMVVFRSLVVPAKAALMNLISIGAAYGVLVA
                     VFQWGWGAGLIGAGKAGPIEAWLPMMLFTILFGLSMDYEIFLLSRVREEYVRTGNNNE
                     AVANGVAATGRVITAAALIMVAVFLSFVFGFPLRQIKMFGLGLAVAVLVDATIVRMVL
                     VPSAMELLGNANWWLPRWLKRILPKVRVEGAPEEPEREPAAV"
     gene            complement(3688..4137)
                     /locus_tag="VEO00_08185"
     CDS             complement(3688..4137)
                     /locus_tag="VEO00_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58013.1"
                     /translation="MSEAPANADEFLADVLAHRGEAEAGVARRLVEWARGQRLDVDRF
                     GKPSGKGNSWVPHVRGLGEMQYLISLWAKSGRVSVMFARLRKERPFNDDVNYAKLERQ
                     FVDLPGWTPKKGSFPDIGLGDLADEAVFARFIAGLEWALGRLRAFSS"
     gene            complement(4139..>4956)
                     /locus_tag="VEO00_08190"
     CDS             complement(4139..>4956)
                     /locus_tag="VEO00_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HYU58014.1"
                     /translation="PPPDRARLSDGKRLIYDGFVQAIAEGTETSAAGILVDEEFGADI
                     ARAARQAGHLLAMPVEKSGQDEFDFEYEDWTDHISRFDPSFSKVLVRYNPDGDGEMNA
                     RQAERLQRLGDWLHQNSRLFLFELLVPAEAAQLESVGGDEGRYDTEVRPALMVRAIAE
                     LQEAGVDPDIWKIEGIDAREDCERISAQTRSGQERENVACVVLGRGADDAAVDHWLKM
                     GSGVPGYVGFAIGRTIWNKPLKAFIAGDADRETAAKQIAANYRRFIDVYESAS"
BASE COUNT          772 a         1856 c         1671 g          657 t
ORIGIN      
        1 gctccccggg cgagagcgag gacgcctcgc tcgtcatccc catcacggtc aggccgtcga
       61 cgctccagcc gatcgcggcc tcgaccaccg accgctgcga cccgagatcg agcgccccgt
      121 cctcgtcgaa cggcgtcggg acgacgaacc acgttcccct gaagggggtc acagcgcctc
      181 tgtcctccgg cgatccggca tcggccggtc cggacgatag gcgttgtcgc tcggcgggat
      241 cctcccggtg agcacgtcgg ccaggtggcg gctcccggca gggccgccca tcactccgcg
      301 cccgctgtag ccggtgttca cccacaggcc ctcgatcccc gccggcccga gcagtggccg
      361 gtggtcgggc gtcatcgtgt agtgaccggc ggccatgaag aactgcgggc tccgttccca
      421 gacgtcgttc cagaacggcg ccacccgggc caccgaggca tcgttccgcg ggtcgagcag
      481 gcggaacgcc agcgcgggat cggtcggaac cgggtcggtc ggcggcgagg gccgctctcc
      541 agggtcgggc ccgagcacga acgcgccacc gagcgccggg cgccagtgcg ccccggtgtc
      601 ctcgtcgatg accatcggcg cgtcggcagg cacctcgggc gcttggggaa cgaccacctt
      661 ctgccgccgg atggtggcca gcggcagtcg ggcgccggcg cgcgccgcga gcgcccctga
      721 gaagggcccg gccgcgatca ccgccgccgc ggtgcggacg acgccgcgcg aggtctcgac
      781 ggcgtcgacc cggtccccca ccaatcggaa gcccgtcacc tccacgcccg gggcgaacgc
      841 ggcaccgagc gccgacgcgc cggcggccag gccgagcgcg gcctggatcg ggtcgagcag
      901 gccgtcggcc tgccggaagc gcgcctgcac aacctccggc gcgacgaacg gccacgacag
      961 ccggacgtcc tcgcccgtga ccaggtccac gtcatcgagg ccccagcccc gctggcgctc
     1021 caccgtctcc cgctgggcgg cggccgtcgc ctccgacgtg gtcagccaca ggtagccggc
     1081 ttcgcgaacc gccgggtcat gctcgcgctg cccggtggcg tcggcgaagt gccggaacag
     1141 atccaccgac tcgcggacga gcgccacctc ctcgtcgtcg tcgagctgga ggcgaaagcc
     1201 gccggtggcc gccgcggtgg tcagcgtgga gagggccggc cggcgctcca ggaccagcgg
     1261 ccgcagcccg gcccgcgccg cgtggaaggc cgtggcggcg ccgacgatcc caccgccgac
     1321 gatcaccacc tcggcggaga ccggcgggtc gtcccggtac gcgatgggca cggcggaacg
     1381 ctaccgcgcg acggctagac ggcggccggc tcgcgctccg gttcctccgg cgctccctcg
     1441 acccgcacct tcggcaggat ccgcttcagc cagcgcggca gccaccagtt ggcgttgccg
     1501 agcagttcca tggccgacgg caccaggacc atccgcacga tcgttgcgtc gacaagcacg
     1561 gccacggcaa gcccgagacc gaacatcttg atctggcgca gcgggaaccc gaacacgaac
     1621 gacagaaaca ccgcgaccat gatcagcgcg gccgcggtga tcacgcgacc cgttgctgcg
     1681 acgccgttcg ccaccgcctc gttgttgttg ccggtgcgga cgtactcctc acggacgcgg
     1741 gacaggagga agatctcgta gtccatcgac aggccgaaca ggatcgtgaa cagcatcatc
     1801 gggagccagg cctcgatggg ccccgccttg ccggctccga tcaggccggc gccccagccc
     1861 cactggaaca ccgccaccag cacgccgtag gcggcgccga tcgagatcag gttcatcagc
     1921 gcggccttcg ccggcacgac cagcgaccgg aacaccacca tcagcaacaa gaaggacagg
     1981 gcgatcacca ccgcgatgag gagcgggagc cggtcggcga tcagccggga gaagtcgacg
     2041 atggccgccg tctgcccccc gaccttgacg tccacgcccg tcccctcggt taccgccggg
     2101 acgacctctc cgcgcagccg gcggaccagg tcgttcgtgg cctggtcctg tggcgcggtg
     2161 gtcgggaaca gccggatcag cgcggcgtcg ccctctgggg tggggatcgg cggcgacacc
     2221 gccgccactc cctccgtctc ccccgccgcg gcggcgaggt cctggagcac ggagaggtcc
     2281 tccggcccgt gaagctctgc cacgagcacc agggcaccgt tgaacccggg cccgaagccc
     2341 tcgctcagca gatcgtaggc ctgccgcgag gtgtagctct cggggttgtt cccagcgtcg
     2401 gcgaagccga gccgcatccg gaacagcggc accgtcaggg tccccatgat cagcagcccg
     2461 atcagcagcg ctgtccaggg gcggcgctgg accaaccggc tccagcggta ccagaaggat
     2521 cgtcggcctg ctccttccgc gcgccgcagc cgcgggatgt ggaagcggtc gatgttgcgt
     2581 ccgacgaatc cgagcactgc cgggagcagc gtgagagacg cgagcatcgt caccagaacg
     2641 gcggcggcgc cgcccacggc gatcccctcg atgaaggaga accggagcag gaggattcca
     2701 aggaacgaga tcaccacgac gctgccggcg aacagcacgg cccggccgga agtggacatc
     2761 gacttggcga cggcctcctc cggctccttc ccctcggaga gcccctgccg gtagcgcgtg
     2821 acgatgaaca gcgcgtagtc gatcccgacg ccgatgccga tcatcgacac cagctgcgtc
     2881 gtgaagttcg gcaccgagag cacgttcgcc gacagcaaca ccaaggcgat gccgatcccg
     2941 atgccaaaca gggcggtcat gatcggcagg cccatcgcca gcaccgaccc gaaggtcagc
     3001 agcaggatga tcatggccgc gagcacgccg acgagctccg cgccccctgg cggctcgaac
     3061 tcggcgaact cgatcaccgg ccctccgaac tcgatctgca accgcgcgcg ctgggccgcc
     3121 tcccccaggt cgcgcaggcg ctcgacctcc tcggccttca tctcggtggc cgggacctcg
     3181 tactggacgg cggcgaagcc gatcctcccg tccggagaga tctgcggtgc ggcgccctcc
     3241 ggggtctcgt aggggctcgc caccgacagg acccgcggaa gctcggcgat gtcggcgaac
     3301 agggcctcca tgtcccggcg cacgagagga tccgcgacgc cgtcggttgc cttgaagacc
     3361 agcacggcct ggtcccccgc cccctgcggg aagcgctcct ccaggaggtc gaagacctcc
     3421 tgcgactcgg tgccggggag attgaagtca gccttgtact cgccacccgc cacgttcgag
     3481 agcgcgatga acgcgacgag cgcgacgatc cacaggacga gcacccgcca gcggtgcgcg
     3541 tagcaccagc gccccagccg ttcgaccatc gggatcgact ccttcgtctc gggcgggcct
     3601 cgtctacggt cgggcaggtc gatcggccaa catcctagcg acgccccggg gaatcatcgg
     3661 ccgccggccc ggccctcccc ctatgtctca ggagctgaag gcgcgcaggc gcccgagggc
     3721 ccactcgagg cccgcgatga acctcgcgaa gacggcctcg tcggccaggt cgcccaggcc
     3781 gatgtccggg aacgagccct tcttcggggt ccaccccggg aggtccacaa actgccgctc
     3841 gagcttggca tagttcacgt cgtcgttgaa cggccgctcc ttgcgcagcc gcgcgaacat
     3901 cacgctgacg cgcccgctct tggcccagag cgagatcaag tactgcatct cgccgagccc
     3961 acgcacgtgg ggcacccagc tgttcccctt gccactcggc ttcccgaagc ggtcgacgtc
     4021 cagccgctgg cctcgagccc actcgaccag ccggcgggcc accccggcct ccgcctcccc
     4081 ccggtgcgcg aggacgtcgg ccaggaattc atcggcgttg gcgggcgcct cggacacgct
     4141 aggaggccga ctcgtagacg tcgatgaagc gacggtagtt ggcggcgatc tgcttggcgg
     4201 cggtctcgcg gtctgcgtcg ccggcgatga acgccttcag cggcttgttc cagatcgtgc
     4261 gtccgatggc gaagccgacg tacccgggca cgccggagcc catcttcagc cagtggtcga
     4321 ccgcggcgtc gtccgcgccg cggccgagga ccacgcaggc cacgttctcg cgctcctggc
     4381 ccgaccgggt ctgcgcggag atccgctcgc agtcctcccg ggcatcgatg ccctcgatct
     4441 tccagatgtc cgggtcaacc cccgcctcct gcagctcggc gatggcccgg accatcaggg
     4501 cggggcggac ctccgtgtcg tagcggccct cgtcgccgcc gaccgactcg agctgtgccg
     4561 cctccgcggg gaccaggagc tcgaacagga acagccggct gttctggtgc agccagtcgc
     4621 cgaggcgctg cagccgctcc gcctggcgcg cgttcatctc gccgtcaccg tccgggttgt
     4681 accgcaccag caccttggag aagctcgggt cgaagcggga gatgtggtcc gtccagtcct
     4741 cgtactcgaa gtcgaactcg tcctgtccgg acttctccac cggcatggcg aggaggtggc
     4801 cggcctgcct ggccgcgcgc gcgatgtcgg cgccgaactc ctcgtccacc aggatcccgg
     4861 cggccgaggt ctccgttccc tccgcgatcg cctggacgaa cccgtcgtag atcaggcgct
     4921 tgccgtcgga cagccgggcg cggtccggcg ggggaa
//
LOCUS       DASPWO010000356         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_134257, whole genome shotgun sequence.
ACCESSION   DASPWO010000356 DASPWO010000000
VERSION     DASPWO010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134257"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VEO00_08195"
     CDS             complement(<1..919)
                     /locus_tag="VEO00_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYU58015.1"
                     /translation="MDFTLNGRPVSVDVPAGATMLDLLREGCGVTSVKDGCAPEGSCG
                     ACTVLVDGKAVVSCAQPAVRAGGKHVTTQEGLSRQERELWADCFVASGASQCGFCSPG
                     IVMKAESLLSKNPEPSRDEVARALAGNLCRCTGYVKIIDAVQLAGRVKGGHPLPAIDT
                     SGRVGSRTARYMGRELALGDKPYINDMSVPGMLHGAIRWSDHPRATVLRIDTSRAEAH
                     PGVMAVVTWRDVPGQRNQGEIYPDWPQFVAEGEVTRYVGDVLAAVAAESRDVARDAAA
                     LIDVEYEVLEPITDPFRALEESAPAIHPDG"
     gene            complement(1058..1603)
                     /locus_tag="VEO00_08200"
     CDS             complement(1058..1603)
                     /locus_tag="VEO00_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYU58016.1"
                     /translation="MGEVALPVANQESPEPRDSGPRILGLPRSRFFGYLFGLLTATCW
                     ATSPIFIRRGLAGLDSPLWGTTIGLTVAAAGFMAWVGWTSRGAIDRPRPRHGGVRLAV
                     FLMAVGGLASGIGALARTTAIDLVAVVIAVPLLQTTSLWTMTFSTLIFGRHAERVSFR
                     LVLGALLVIGGAALVIVGQNL"
     gene            complement(1607..2026)
                     /locus_tag="VEO00_08205"
     CDS             complement(1607..2026)
                     /locus_tag="VEO00_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYU58017.1"
                     /translation="MAGAAWALFAAISFSLGHIALARGLPSLGVARGTAILLLASAIT
                     VAGLALAVDGVAPLRQAPLSAVLWFTAAGAIHYGSWGFMNQSIRLVGPSRMAAITGVT
                     PLFAALLAVITLGESVNALVGLGIALIVAGTYLIARS"
     gene            2341..3003
                     /locus_tag="VEO00_08210"
     CDS             2341..3003
                     /locus_tag="VEO00_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYU58018.1"
                     /translation="MATYVLIHGAGDVGWYWHLVEAALRDRGHDVVAPDLPGNDDSAG
                     LWTYADAVLEAIGDRTELIVVAQSFGAFTGPLVCDRRKADLLVLVAGMIPSPGEAPRD
                     WWANTGYDREPRERYDDEIALFYHDVPPELASEALRRGRAQSETPTREPWPLAAWPAV
                     PTRFLLCRDDRLFPAPFLRRVARERLGITPDEIDGGHCVALSRPRELADRLEGFRVHP
                     RR"
BASE COUNT          524 a         1124 c         1138 g          462 t
ORIGIN      
        1 tgccgtcggg gtggatcgcc ggcgcggact cctcgagggc gcggaaggga tcggtgatcg
       61 gttcgaggac ctcgtactcc acgtcgatca gcgctgcggc gtcccgggcc acgtccctgg
      121 attcggcggc cacagcggcc aggacgtcgc cgacgtaccg ggtgacctct ccctccgcca
      181 cgaactgcgg ccagtccgga tagatctcgc cttggttccg ctggccgggg acgtcccgcc
      241 aggtaaccac ggccatgacg ccgggatgcg cctcggcccg tgatgtgtcg atcctgagca
      301 ccgtcgcccg gggatggtcg gaccaccgga tggccccgtg gagcatcccc ggcacggaca
      361 tgtcgttgat gtagggcttg tcacccagtg ccagctcgcg tcccatgtac cgggcggtcc
      421 ggctcccgac gcgtccgctg gtgtcgatgg cggggagcgg gtggccgccc ttgacccgac
      481 cggccagctg gaccgcgtcg atgatcttca cgtaccccgt gcaccggcac aggttgcccg
      541 ccagggcccg ggccacctcg tcgcgggagg gctccgggtt cttcgacagg agcgactccg
      601 ccttcatgac gatgcccggc gagcagaacc cgcattggga tgcaccggag gccacgaagc
      661 agtccgccca cagctcccgt tcctgccgcg agagaccttc ctgcgtggtg acgtgcttcc
      721 ctccggctcg aaccgcgggt tgggcgcagg acacgacggc cttcccgtcc accaggacgg
      781 tgcaggcgcc gcacgagccc tcgggcgcac acccgtcctt cacggaggtg accccgcacc
      841 cctcccggag gagatcgagc atcgtcgcac ccgccggaac atcgacggag actggacggc
      901 cgttcagggt gaaatccacg agattctcct tcgtgacctc gggagcccac tgcgcgagcg
      961 tgacccacga ccccgacgct tatcgtgtgc ccgcttcaac aaaatgtcaa ccttcgcacg
     1021 cagcggatta cgggtccgaa cgacgcgatc cggttcctca gaggttctgt cccacgatga
     1081 ccagcgcggc cccaccgatc accagcagcg cccccagcac gagccggaac gagacccgct
     1141 cggcgtgccg cccgaagatc agcgtggaga acgtcatcgt ccacagcgag gtggtctgca
     1201 acaggggaac cgcgatcacc acggcgacca ggtcgatcgc ggtcgtcctg gccagggctc
     1261 cgatgcccga cgcgagcccg ccgaccgcca tgaggaagac ggcgagccgg acgcctccgt
     1321 ggcgcggccg gggacgatcg atggcccctc gagaggtcca tccgacccag gccatgaagc
     1381 cggcagcggc cacggtcagg ccgatggtgg tcccccagag cggcgagtcc agccccgcca
     1441 gcccccttcg gatgaagatc gggctcgtcg cccagcacgt cgcggtgagc aggccgaaca
     1501 ggtagccgaa gaaacggctc cggggaaggc ccaggatcct gggtcccgag tcccggggct
     1561 ccggcgactc ctggttcgcg acgggcaggg ccacctcgcc caccggctag ctccgcgcga
     1621 tcaggtaggt gccggccacg atcagcgcga tgcccaggcc gacgagcgcg ttgacgctct
     1681 ctccgagcgt gatcaccgcg aggagcgcgg cgaacagcgg ggtgacgccg gtgatggcag
     1741 ccatccggga cggcccgacc agccggatgc tctggttcat gaagccccac gagccgtagt
     1801 ggatggcgcc ggccgccgtg aaccagagga cggcggagag aggagcctgc cggagcggcg
     1861 cgacgccgtc cactgccagc gcaaggccgg cgacggtgat cgcgctcgcc aggagcagga
     1921 tcgcggtgcc ccgggccaca ccgagcgacg gcaggccgcg cgccagggcg atgtggccga
     1981 gcgagaagga gatcgccgcg aacagcgccc atgcggcacc agccattcgc tcaggcgaga
     2041 ctcgacacgg tctcgagtcg tgaggagagg atcggctttg cgggggccat cggcgcggcc
     2101 ggaccataca tcggcccggt tgggcattca acgcaccgac gcgagcgagg cgcagccagg
     2161 gctcaggcct ggacgggccg tcagggggga aggtatagtg ccgcgctcgc tgcttgcctt
     2221 cctgaagttg gctggaacca gctgcggagt tggaggtggc atgccgatcc tggtgccctg
     2281 agccctggcc tccgctccgc gttcggcctt cgtcatctcg tccatgctgg gaggtgacga
     2341 atggcgacgt acgtcctgat ccacggcgcc ggcgatgtcg gctggtactg gcacttggtg
     2401 gaagccgcac tccgtgaccg aggtcacgat gtcgtggcac cggatcttcc gggcaatgac
     2461 gactccgccg gactctggac gtacgcggac gccgtgctcg aggcgatcgg cgaccgcacc
     2521 gagctgatcg tggtggcgca atcgttcggc gcgttcacgg gcccgttggt ctgtgaccgg
     2581 aggaaggcgg acctgctcgt cctggtggcg gggatgatcc cgtcgccggg ggaggcgccg
     2641 cgcgactggt gggccaacac gggatacgac cgggagccac gggagcgata cgacgacgag
     2701 atcgccctct tctaccatga cgttccgccg gaactggcgt ccgaggcgct gaggagggga
     2761 cgcgcgcagt ccgagacgcc gacgcgggag ccgtggcccc tcgcagcgtg gcccgccgtg
     2821 ccgacgcggt tcctcctgtg ccgagacgac cgcctcttcc cggccccctt ccttcgccgg
     2881 gtcgctcggg aacgtctggg gatcacaccg gacgagatcg acggcggaca ctgcgtcgcc
     2941 ctgagtcgcc cgagggagct ggcggaccgt ctcgagggct tccgggtcca cccacgtcga
     3001 tgaggccggc gccgcgctcg acacgggccc tgggtgtgga cggtcgacgg gccgacgcca
     3061 cagagccgtc acgacgcccg tgcttcgatc acctggatgg cagctgcgtc catgcgatga
     3121 atcatccggt ccgctatgcg cgagacgagg attcctcaaa gggcggagtc atgggttcgg
     3181 tttgccaaca ccaacaaggc gacgacccgc gcaccagaag cggctgtccg cgttgagacc
     3241 cggtcgtg
//
LOCUS       DASPWO010000357         4801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_645134, whole genome shotgun sequence.
ACCESSION   DASPWO010000357 DASPWO010000000
VERSION     DASPWO010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645134"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1192..2391
                     /locus_tag="VEO00_08215"
     CDS             1192..2391
                     /locus_tag="VEO00_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HYU58019.1"
                     /translation="MDALAEQPDGRIEEDFAELQRAAQLIELERLRRLAEIERRGLYQ
                     RDGHLSATAWLSSVFRLGWGAARDNVAVARALEDMPRTREALEGGDLSLPAVRILAAA
                     HDVDPEAFARCEAQLVETARVHSVRELRRVAAYFRQGVERDRELDGDEAARARRRLHA
                     SVTFMGMVRVDGDLDPETGETLLTALRAVLDAEARSGGDLDGRTPGQRRADALGEIVR
                     QWLELAERPQVAGEKPHVTVTVGADALADPDRRDAVRRPADGGEIDAGAELDHIGPVG
                     SGLARRLACDASIRRVVMAGPSEPLDVGRKTPVVSPAMRRALILRDQGCRFPGCDRPH
                     TWCDAHHVVHWADGGPTVLLNLVLLCRRHHRMVHTRNRFRLELFDGRPLFRRPDGSVL
                     EDDRAPP"
     gene            complement(2667..3890)
                     /locus_tag="VEO00_08220"
     CDS             complement(2667..3890)
                     /locus_tag="VEO00_08220"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HYU58020.1"
                     /translation="MTNEFATVGYRAHSQVHGALDPDVDVEALSGVARDALEAGVEID
                     GDEVEIPLNVAFGNPGLLRLVGLEAVLAGLGAEREYANDEMLDNQVRSVLFQVPGPDI
                     EDPAACLDGEGLPGCFSGVLDLGAIDVARGRDNGIPTCNELRAAYGLPPVTSFAELTG
                     EATDAFPDDPAIDGRDPLNDPDILGFVSLRDDEGREVAPGSPEAEDDVVSVVRRTSLA
                     ARLKAIYGTVDRVDAFVGMNSEPHVWSSELGELQRAMWATSSRRCETAAGSSSGTTRC
                     SARSPAPTASTSAAAWRGSSATTRTCPTGICRTTCSSPVWPPRTSAVRRRQGITRTGG
                     TSIGATSADGAARTGGGAGTLGSTERRAARKRRAAVAAPTSRHRRFRLSAAGRVARGV
                     LARLVVIALARVGRV"
     gene            complement(3803..>4801)
                     /locus_tag="VEO00_08225"
     CDS             complement(3803..>4801)
                     /locus_tag="VEO00_08225"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HYU58021.1"
                     /translation="LAFRYPPRAGGDLSRRVLDGPSLDGSGNNLVDPSLGQAGLPYPR
                     LTGARYADGVAAMADGPDPRFISNRIFNDGNQNVFSPRGVSQWGFVWGQFLDHTFGKR
                     LEADEEAAIAFDPADPLEAFRNDLGNIAFERSAAAAGTGQKAVREQVNTVSSYIDAWA
                     VYGGTAGRLDWLRAGPADGDPTNNRALLPTDDGYLPTAASRPGVNAPAMDLMGRLMTD
                     PSVAVVAGDVRANENLALTAVQTLFVREHNRIVAALPDGLPEEEKFDIARRVVAAEQQ
                     WITYTELLPAMASSCPRTAATTRRSTRASRTSSPRWGTGRTPRSTGRSIRTSTSRR"
BASE COUNT          633 a         1721 c         1722 g          725 t
ORIGIN      
        1 cgtccattcg aagctgagct cattcgaggt gaccactcgc aggtgagaga acacccggct
       61 cctcccgtcc gctcggcgca atatcagcgt gccggttgcc ccctgagccc gaagggtcgc
      121 gcctccacct cctcgagcgg cgtcggcgag cgctcggagg aagttcacag caagcgggat
      181 cgcgaccttg gtcttcgtgg tggttgccct ggtcggcggt gccctggctt ctgcctcttc
      241 agtagactct gtctgcactt cttggcctcc ttccgttgtt tggcctcgaa gctggcgcga
      301 tccgagcgca cccgacggtc ctcgagcgtc acgtcgggat tcttgaaagc gcgctctctc
      361 cgcacgcgat cccagttcaa tcgtgccatc tacccaccct ccgcctgatg ggacccgttg
      421 gctcgccgca tctatgcttc ccacgttcga tgaagcgccg agttccgtac ccgccggcgt
      481 ggtcaagtcc tgagtttcct gtaattgcag cggcctgagt cacgcagcgc tcctcttcct
      541 tgtcttgcct tgtcttgcga acctcccttc tcattggggg gcatcagatc gatcccgagc
      601 tatcggcggg agcctggtga ttctccacgg cgcccaacta tccggagccc cgcccgggct
      661 caagcggagc gtgtcggtgg cggccgctac gctgtccgga gcctccccca cgaggcgtcg
      721 aggagcggcg tggacgttcg cgtgtggggg tggcccggga ccggctgatc gagctggtgg
      781 tcgagccatt gccgggcggc tccggcctcc ggatcggggg cctgcctccc ggacgagccc
      841 ccatcgccag ggatcgcgtc cgcgtggcca tcctgacctg cggcctggcc gacgaggcgc
      901 cctcggtggc ggtccggctc gagcccccct cggtggcggc tccacggccg acctcgacct
      961 gccgatcgcc ctggccgccc tggccgcggc gggggcggtc cccggaagcc ggcgctggat
     1021 cttcgcggtg ggccgtctgg gcctcgacgg gagggtccac gctccgggtc ttcatggtcg
     1081 cctgagcctc ggggacgtcg cggaaacgtg tcgtaacccc cctggtagaa tccgaacaca
     1141 tgttcgagag gaacgggtga gcgagctcgg tcggcgatcg acgggttccg ggtggacgcc
     1201 ctggcggaac agccggacgg gcggatcgag gaggacttcg ccgagctgca gcgggccgcc
     1261 cagctgatcg agctggagcg gctcaggcgc ctcgccgaga tcgagcgacg ggggctgtac
     1321 cagcgcgacg gacacctgtc ggcgacggcg tggctgtcga gcgtgttccg cctgggctgg
     1381 ggggcggccc gggacaacgt ggcggtcgcg cgggccctgg aggacatgcc ccgcacccgg
     1441 gaggccctgg aggggggcga cctgtccctc ccggcggtgc ggatcctggc cgccgcccac
     1501 gacgtggacc ccgaggcgtt cgcgcggtgc gaggcccagc tggtggagac cgcccgggtg
     1561 cactccgtgc gcgaactgcg gcgggtggcg gcctacttcc gtcagggggt cgagcgcgat
     1621 cgcgagctcg acggggacga ggcggcccgg gcccggcgac gcctccacgc gtcggtcacg
     1681 ttcatgggga tggtccgtgt ggacggcgac ctcgatcccg agaccgggga gacgctgctc
     1741 accgcgctgc gggccgtgct cgacgccgag gcgaggtccg gcggcgacct cgacggccgc
     1801 acgccggggc agcgacgcgc ggacgcgctc ggggagatcg tccggcagtg gctggagctc
     1861 gcggagcggc cgcaggtcgc cggcgagaag ccgcatgtca cggtgaccgt cggcgccgac
     1921 gcgctggcgg acccggaccg tcgggacgct gttcgccgtc cggcggacgg cggcgagatc
     1981 gatgcgggcg ccgagctcga ccacatcggg ccggtcgggt cgggcctggc gcggcggctg
     2041 gcctgcgacg cctcgatccg gcgggtggtg atggccgggc cctcggagcc gctggacgtc
     2101 gggcgaaaga cgcccgtggt gtcaccggcg atgcgccggg cgctcatcct gcgggaccaa
     2161 ggctgccggt tccccggatg cgatcgtccg cacacctggt gcgacgcgca ccacgtggtc
     2221 cactgggccg acggcggccc gacggtgctt ctgaacctgg tcctgctctg ccgtcgccac
     2281 caccggatgg tccacacgcg gaaccgcttc cggctggaac tgttcgacgg ccggccgctg
     2341 ttccgacgcc cggatggatc ggtactcgag gacgaccgcg ccccgccctg atcgccccgg
     2401 gtgggccgca gaactcaggc gactcatggg ctgctctcat ggcggcctca ggtccgggtg
     2461 gtgtccttct cggcgtccac tgacgtcgag gaagggaggt gaccatggct accagacttc
     2521 gcagggcgat cgtggcgggg agcctcgcgg gcagcgcact gttcctcggg gcggtcggga
     2581 tcgggcacgc gtgggcgaac tcgtcgggga catcgagctc ctcgggcacc gaggcgacga
     2641 tcacctcgga caactcggat acgagctcag actcgtccga cgcgagcgag tgcgatcacg
     2701 accagtcgag cgaggacacc tcgagcgaca cgaccagcag ctgacaaccg gaaacggcgg
     2761 tgacgggacg tcggggcggc aaccgccgcc cgacgtttcc gtgccgcccg ccgttcagtg
     2821 gaaccgaggg tgcctgctcc cccgcccgtc ctcgcggcgc catccgcgct cgtggcgccg
     2881 atgctcgtcc ccccggtacg ggtgatgccc tgccggcgcc tcacggcgct cgtcctcgga
     2941 ggccacaccg gcgaagaaca cgttgtcctg cagatcccgg taggacaggt ccgagttgtc
     3001 gccgatgacc cgcgccaggc tgcggcggaa gtcgatgccg taggcgcggg cgatctcgcc
     3061 gagcatcggg tcgttcccga agaagaaccg gccgccgtct cgcagcgccg tgaactggtg
     3121 gcccacatcg cccgctgcag ctcgccgagc tcgctgctcc agacgtgcgg ctcggagttc
     3181 atcccgacga aggcgtccac gcggtccacc gtcccgtaga tggccttcag gcgcgccgcc
     3241 aggctggtgc gccgcacgac gctcacgacg tcgtcctcgg cctccggcga ccccggcgcg
     3301 acctcccgcc cctcgtcgtc gcgcagtgag acgaagccca ggatgtccgg gtcgttcagc
     3361 gggtcgcgcc cgtcgatcgc cgggtcgtcg gggaacgcat cggtcgcctc cccggtcaat
     3421 tcggcgaacg aggtcaccgg cggcaggccg taggccgcgc gcagctcgtt gcacgtcggg
     3481 atcccgttgt cccggccgcg ggccacgtcg atcgcgccca gatcgagcac cccgctgaag
     3541 catcccggca gcccctcccc gtccaggcag gccgccgggt cctcgatgtc cggccccggg
     3601 acctggaaca gcacgctgcg cacctggttg tcgagcatct cgtcgttcgc gtactcgcgc
     3661 tcggcgccga ggccggccag cacggcctcg agcccgacca gccgcagcag gccggggttg
     3721 ccgaaggcga cgttcaacgg gatctccacc tcgtcgccgt cgatctcgac accggcctcc
     3781 agcgcgtcac gcgccacgcc gctcagcgcc tcgacgtcga cgtccggatc gagcgccccg
     3841 tggacctggg agtgcgcccg gtaccccacc gtggcgaact cgttcgtgat gcgcgggtcg
     3901 agcgacgggt cgtagccgcc gtacggggac agctcgacgc catcgccggg agcaactcgg
     3961 tgtaggtgat ccactgctgc tcggcggcga ccacccggcg ggcgatgtcg aacttctcct
     4021 cctccggcag cccgtccggg agcgccgcca cgatgcggtt gtgctcccgt acgaacaggg
     4081 tctgcaccgc ggtcagcgcg aggttctcgt tcgcgcgcac atcgccggcc accaccgcga
     4141 ccgacgggtc ggtcatcagc cggcccatca ggtccatcgc cggcgcgttc acgccggggc
     4201 gggaggcggc cgtcggcagg tacccgtcgt cggtcgggag cagcgcgcgg ttgttcgtcg
     4261 ggtcgccgtc ggcggggccg gcccgcagcc agtcgagccg cccagcggtc ccgccgtaga
     4321 cggcccaggc gtcgatgtac gagctgacgg tgttcacctg ctcgcggacc gctttctggc
     4381 cggtgccggc ggcggccgcc gaccgctcga acgcgatgtt cccgaggtcg ttgcggaacg
     4441 cctcgagcgg atccgccggg tcgaaggcga tcgccgcctc ctcgtcggcc tccagccgct
     4501 tgccgaacgt gtgatccagg aactggcccc agacgaaccc ccactggctc acgccgcggg
     4561 gcgagaagac gttctggttc ccgtcgttga agatccggtt gctgatgaag cgggggtcgg
     4621 gcccgtccgc catcgcggcg acgccgtccg cgtagcgggc gcccgtcaac ctcgggtacg
     4681 gcagcccggc ctggccgagc gagggatcca cgaggttgtt gccggaaccg tccaggctgg
     4741 ggccgtcgag cacgcgccgg gagaggtcac cgcccgccct cggcgggtag cggaacgcca
     4801 g
//
LOCUS       DASPWO010000358         5315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_820629, whole genome shotgun sequence.
ACCESSION   DASPWO010000358 DASPWO010000000
VERSION     DASPWO010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820629"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VEO00_08230"
     CDS             <1..152
                     /locus_tag="VEO00_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYU58022.1"
                     /translation="KAGEVRNDVPPDELASYCMHALGAATPHRSKAAVRRLVSLTMAG
                     LRPPR"
     gene            complement(171..1382)
                     /locus_tag="VEO00_08235"
     CDS             complement(171..1382)
                     /locus_tag="VEO00_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYU58023.1"
                     /translation="MTDPHRRWRSTIRVRLSLLYAGAFFLAGAALVGLMYFFMGQALD
                     RQVTARVGIIQHLPESNPTEPGSLRSAHQQARELRAQFERDREGTLNSMLVTSLVALG
                     VVGVAAGGLGWLVAGRALRPLQQITATARRVADRSLHERISLDGPDDEIKDLADTFDA
                     MLERLDRSFDSQRRFVANASHELRTPLTINRTLVEVALDNPRADETVRQLGANLLAVN
                     ERHERLIDGLLTLASSEQGITDPTPVDLSDVAGHIVAESEDAARAAGIHVRTDLRPAS
                     VTGDPVLLERLVQNLLDNAIRYNLPGEGEITVATNALAESVHLTVENTGPLVPSYEIP
                     SLFEPFRRIPTTERLADSTASVNRGAGLGLSIVRSVAHAHGGDVDASPREGGGLTVRV
                     RLPALPERSTG"
     gene            complement(1379..2035)
                     /locus_tag="VEO00_08240"
     CDS             complement(1379..2035)
                     /locus_tag="VEO00_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU58024.1"
                     /translation="MRVLVVEDERLLADTIADGLRRQALAVDVAYDGEAALERLAVND
                     YDVVVLDRDLPIVSGDEVCTSVVDSGADTRILMLTAATAVPQRVAGLGLGADDYLTKP
                     FAFAELAARILALGRRARPAAPPVLERAGIRLDQHRRTVTRDGLPVELSRKEFAVLAE
                     LLRAEGGVVSAEQLLEKAWDEHIDPFTAVVRFTIMMVRRKLGDPPVIDTVPGTGYRIS
                     "
     gene            2151..2765
                     /locus_tag="VEO00_08245"
     CDS             2151..2765
                     /locus_tag="VEO00_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58025.1"
                     /translation="MTATPRDGGARGPVRAPDDDAVGSPSRFGLRLRLLAVIAAAGIV
                     AAACGSSGGADAPAPSATVEAALLAFSQCMREQGITDMPDPIVDSDGNVRVQQPPGGH
                     GSGAHDAFAEARAKCDTYLRGVTQRFSHGDVTKTQDQFLRLAQCMRDRGIDVPDPDFS
                     GGGHDPGAEFLDAINRSDPAVQKALQACERRVFGSGGIGHAGGH"
     gene            2810..3859
                     /locus_tag="VEO00_08250"
     CDS             2810..3859
                     /locus_tag="VEO00_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYU58026.1"
                     /translation="MAALLVGAGGGWLLAPRSTDGAGGTGATPATFGKVVRTDVQART
                     DLTGGLGHVADRTIACRLSEPEGGATLTGLAAQGTTVRPGGMLFSVDERPVALLLGPT
                     PAWRTLRVGVVGTDVSQLERNLESLGYGGLTVDDTFTSATGAAVRRWQRDIGTRADGV
                     VDLGEVVFLPSAVRIDQDLLTPGDAVSSGTRVLAVGPIAPVVTVDLEPTQLSLVSVGD
                     RVIVRLAGGTETPGTVVSVGQEGATFGGPQPGETEAIPATVSLERPADAAGLAGSSAT
                     VSVVTDTSEDALAVPVTALLAVPGGYVVEVDRGDRIRAVTVTPGLFDGITGLVEIMAT
                     GLEPGDRVVVAEAGA"
     gene            3922..4659
                     /locus_tag="VEO00_08255"
     CDS             3922..4659
                     /locus_tag="VEO00_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU58027.1"
                     /translation="MALRDVVRDYAGPPPVRALHGVSLHIADGELAAIVGPSGSGKST
                     LLHIMGTLDRATSGQVRIAGRDVATLSDAELSALRARSIGFVFQEFFLLEAETALDNV
                     ADGLLYTGIPARDRRLLARDALERVGLGHRIGHPATKLSGGERQRVAVARAIVGRPAI
                     VLADEPTGNLDSRSSEEIVGLLEDMNRDGITIVVITHNPEVADRFPRRIGIRDGGIEG
                     DATAAVRPDAPDASRPLHPGAAEGGEP"
     gene            4656..>5315
                     /locus_tag="VEO00_08260"
     CDS             4656..>5315
                     /locus_tag="VEO00_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU58028.1"
                     /translation="MTGDLTPGRLHPADAVRTSTVGLRTRRTRSLLSALGILIGIAAM
                     IATLGLSESSKSDLLAQLDRLGTNLLRVQASQGLGLGTARLPETALAMSGRLDGVERV
                     SEQVDGAANVFRTDYIPTTRTGGLTVKAVDTGILGTLKGTVAEGRFLDDATARYPVAV
                     LGSVTAERLAIRDLTEQPRVWIGNQWFSVIGILHTLDLNPDLDRSAIIGVPAAQAYLG
                     SD"
BASE COUNT          762 a         1867 c         1935 g          751 t
ORIGIN      
        1 cgaaagcagg tgaggtccgg aacgacgtgc cgcccgacga gctcgccagc tattgcatgc
       61 acgccctggg ggcagcgacc ccccaccgtt cgaaggccgc ggttcgtcgc ctggtgtctc
      121 tgaccatggc ggggttgcgg ccaccacgct gattcacgct ggggtgaccc ctatccggtg
      181 gatcgttccg gcaacgccgg cagccgcacc cggaccgtga ggcctccgcc ttccctgggg
      241 gaggcgtcga cgtctccacc gtgagcgtgc gcgacggagc gcacgatgga cagcccgagg
      301 ccggccccac ggttgaccga tgcggtcgag tcggcgagtc gttccgttgt cgggatccgg
      361 cggaagggtt cgaacaggct cgggatctcg taggacggca cgagcggccc ggtgttctcc
      421 acggtgaggt gaacgctctc cgcgagcgcg ttcgtggcga ccgtgatctc gccctctccg
      481 ggcaggttgt agcggatggc gttgtccagc aggttctgga cgagtcgctc cagcagcacc
      541 gggtcgccgg tcacggaagc cggtcggaga tcggtccgga cgtggatgcc ggctgcacgg
      601 gcggcgtctt cggattccgc gacgatgtgg cctgcgacgt cggagaggtc cacgggggtg
      661 ggatcggtga tgccctgctc gctgctggcc agggtgagca ggccgtcgat cagccgttcg
      721 tggcgctcgt tgacggcgag caggttcgcg ccgagctggc ggaccgtctc gtccgcccgg
      781 gggttgtcga gcgcgacctc gacgagggtg cggttgatcg tgagcggcgt gcggagctcg
      841 tgggaggcgt tggcaacgaa tcgccgctgg ctgtcgaagg agcgatccag ccgctcgagc
      901 atggcgtcga aggtgtcggc gaggtccttg atctcgtcgt ccggcccatc gagggagatg
      961 cgttcgtgga ggctccggtc ggcgacccgt cgggccgtcg ccgtgatctg ttgcagggga
     1021 cgcaacgccc gcccggcgac cagccatccg agccctccgg cggcgacacc gaccacaccg
     1081 agcgcgacga gcgacgtgac gagcatggag ttcagggtgc cctcccggtc ccgttcgaac
     1141 tgggcgcgca gctcccgtgc ttgctgatgg gcactccgga gcgagcccgg ttcggtgggg
     1201 ttcgactccg ggaggtgctg gatgatcccg acccgcgccg tgacctgacg atcgagggcc
     1261 tgtcccatga agaagtacat gagaccgacc agcgccgccc ccgccaggaa gaacgccccc
     1321 gcgtagagca gcgagaggcg gacccggatc gtggaacgcc atcggcggtg cgggtcggtc
     1381 atgagatgcg gtaccccgta ccgggcaccg tgtcgatgac cggaggatcg cccagcttcc
     1441 ggcgcaccat catgatggtg aaccggacca cggcggtgaa cgggtcgatg tgctcgtccc
     1501 acgccttctc cagcagctgc tccgccgaga ccacgccgcc ctcggcccgc aggagctcgg
     1561 cgaggacggc gaactccttg cgggacaact cgacggggag gccgtcgcgg gtgaccgttc
     1621 ggcgatgctg gtccaggcgg atgccggcgc gttccaggac cggaggggcg gcgggtcggg
     1681 cccgtctgcc caaggccagg atccgggcgg ccagctcggc gaaggcgaac ggcttcgtca
     1741 ggtagtcgtc cgcgcccaac ccgagtccgg ccacccgctg ggggaccgcg gtggcggccg
     1801 tgagcatcaa gatccgggta tcggcgcccg agtcgaccac ggacgtgcac acctcatcgc
     1861 ccgagacgat ggggaggtcg cggtcgagga ccaccacgtc gtagtcgttg acggcgagcc
     1921 gctccagcgc ggcctcaccg tcgtaggcga cgtccacggc caaggcctgc cggcgcagcc
     1981 catcggcgat ggtgtccgcg agcagccgct cgtcctcgac caccaagaca cgcatgatcc
     2041 catcatgcct ggtcagggcg ttagggggct gtgaggacag ccctcacgcc ggtcgaacgc
     2101 cccgaggtgt cggatagggc cgtcacgtgg cgatcgagga ggcgacatcg atgacggcga
     2161 caccgaggga cggcggagca cgggggcccg ttcgtgctcc cgacgacgat gcggttggat
     2221 ctccatcgcg cttcggcctg cgcctgcggc tgctggcggt gattgccgcc gccggcatcg
     2281 tcgcggccgc atgcggctcc agcggcggcg ccgacgcccc agccccgagc gcgacggtgg
     2341 aggcggcgct cctcgcgttc tcccagtgca tgcgcgagca gggcatcacc gacatgcccg
     2401 atccgatcgt ggactccgac gggaacgtcc gggtccaaca gccgccgggc gggcacggct
     2461 ccggggcaca cgatgccttc gcggaggccc gggccaagtg cgacacgtac cttcgggggg
     2521 tcacccagag gttcagccac ggcgacgtca ccaagaccca ggaccagttc ctgcgcctgg
     2581 cccaatgcat gcgcgatcgc gggatcgacg tccccgatcc cgacttcagc gggggcggcc
     2641 acgaccccgg ggccgagttc ctggacgcga tcaaccgctc ggatccagcc gtgcagaagg
     2701 cgctgcaggc gtgcgagcga cgtgtcttcg gctcgggggg catcggtcac gctgggggtc
     2761 actagcgatg gccgttcgcc gccggcggag gaggcgttgg gcgctgatcg tggcggcctt
     2821 gctggtcggc gcgggcggcg gatggctcct cgccccgcgc tccacggacg gggcgggcgg
     2881 caccggcgcc accccggcga cgttcgggaa ggtcgtgcga accgacgtcc aggcgagaac
     2941 cgacctcacc ggcgggctcg gccacgtggc cgatcgcacg atcgcctgcc gactctccga
     3001 acccgagggc ggcgcgacgc tgaccggcct ggcggcccag ggaacgacgg tccgaccagg
     3061 tgggatgctg ttttccgtcg acgagcgacc ggtcgccctg ctgctgggac cgacgcccgc
     3121 ctggcgaacc ctccgagtgg gcgtggtcgg gaccgacgtc agccagctcg aacgcaatct
     3181 cgagtcgctg ggctacggcg gtctcaccgt cgacgacacc ttcacgtccg cgacgggcgc
     3241 ggcggtccgg cggtggcagc gcgatatcgg caccagggcc gacggcgtcg tggacctcgg
     3301 cgaggtggtg ttcctcccga gcgccgtgcg gatcgatcag gacctgctga cgccggggga
     3361 cgccgtgagc tccggaaccc gggtgctcgc ggtcggaccg atcgctccgg tggtgacggt
     3421 cgacctcgag cccacgcagc tctcgctggt ctcggtcgga gatcgggtga tcgtccggct
     3481 cgccggcggc acggagaccc ccgggaccgt ggtctcggtg ggacaggagg gggccacctt
     3541 cggcgggccc cagcccggcg agacagaagc gattcccgcg accgtgtcgc tcgagcgtcc
     3601 cgcggacgcc gccgggctcg ccggctcctc ggcgacggtc agcgtggtca ccgacaccag
     3661 cgaagacgcg ctcgcggtcc cggtgaccgc gctcctggcg gttcccggcg gctacgtggt
     3721 cgaggtcgac cgcggggacc gcatccgggc ggtgacggtc acgcccggcc tcttcgacgg
     3781 gatcacgggc ctggtcgaga tcatggcgac ggggctcgaa ccgggcgacc gcgtcgtcgt
     3841 ggcagaggcc ggagcgtgag cctcttcgcc cgcggccggc ccgaagagcc gccgaccgaa
     3901 acgcccggca ccggcggggt catcgcgctt cgcgacgttg tcagggacta cgccggcccc
     3961 ccgccggtgc gggccctgca cggcgtgagc ctgcacatcg ccgatgggga gctggcggcg
     4021 atcgtggggc cgtcgggatc ggggaagtcg accctgctgc acatcatggg gacgctcgac
     4081 cgggccacct ccggccaggt gcgcatcgcc gggcgcgacg tggcgacgct ctccgacgcc
     4141 gagttgtccg cgctgcgcgc caggagcatc gggttcgtct tccaggagtt cttcctcctc
     4201 gaggccgaga cggccctcga caacgtggcc gacggcctgc tgtacacggg catccccgcc
     4261 cgcgaccgcc gtctgctcgc gcgcgacgcg ctcgagcgcg tgggactcgg gcaccggatc
     4321 ggccatccgg ccaccaagct ctcgggcgga gagcggcagc gcgtcgcggt cgcccgggcc
     4381 atcgtcggtc ggcccgcgat cgtgctcgcg gacgagccca ccggcaacct ggacagccgc
     4441 tcgagcgagg agatcgtcgg gctgctcgag gacatgaacc gggatgggat cacgatcgtg
     4501 gtgatcaccc acaaccccga ggtggccgat cggttcccgc gacgcatcgg catccgcgac
     4561 ggcggcatcg agggcgacgc cacggcggca gtccggcccg acgcgcccga cgcgtcccgt
     4621 cccctgcacc ctggtgccgc cgagggaggg gagccatgac cggcgacctc acccccggcc
     4681 gcctccaccc cgccgacgcg gtccgcacgt ccacggtcgg tctgcggacc aggcgaaccc
     4741 gctctttgct gtcggcgctc gggatcctta tcgggatcgc ggcgatgatc gccacgctcg
     4801 gcctgtcgga gtccagcaag tccgatcttc ttgcccagct cgatcgcctc gggaccaacc
     4861 tcctgcgggt gcaggccagc caaggcctcg gcctgggcac cgcccggctc cccgagacgg
     4921 ccctcgccat gagcggccgc ctcgacggag tcgaacgcgt ctcggagcag gtggacggcg
     4981 cggcgaacgt gttccggact gactacatcc ccacgaccag gaccggtggc cttacggtca
     5041 aggccgtcga taccggcatc ctcggcaccc tgaaggggac ggtcgccgag ggccggttcc
     5101 tcgacgacgc gacggcccgg taccccgtgg ccgtcctcgg ctccgtgaca gcagagcgcc
     5161 tggccatcag ggacctcacc gaacagcccc gagtctggat cggcaaccag tggttctcgg
     5221 tcatcgggat cctccacacg ctcgacctca acccagatct cgaccggtcg gcgatcatcg
     5281 gggtccccgc cgcccaggca tacctcggct ccgac
//
LOCUS       DASPWO010000359         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_901805, whole genome shotgun sequence.
ACCESSION   DASPWO010000359 DASPWO010000000
VERSION     DASPWO010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901805"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VEO00_08265"
     CDS             complement(<1..729)
                     /locus_tag="VEO00_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYU58029.1"
                     /translation="MADRPNVLLVLMDTARADAFEPWGAPAGSTPVVAELARRGTAFP
                     MAVAPSSWTVPSHASMFTGLLPRAFGFTPGLWAPRELRTRMRSLFDRHRDRTLAEVLR
                     HGGYATGAVSTNPIVNSATGFSTGFDWFGDPGRTRFTRLQRQRQRAAARRGPAGAARR
                     ARDRAAWWRARDDNGAVHAGRALRRWLDHSGGAKPFFAFVNLMETHRPYYPPRPWNDL
                     GPAARWQAVGDVFRWQGHIEVVLVS"
     gene            complement(738..>2002)
                     /locus_tag="VEO00_08270"
     CDS             complement(738..>2002)
                     /locus_tag="VEO00_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYU58030.1"
                     /translation="APALWTVPDLRTVVPAVMARHADRTLAEVLRRAGYGTRALSTNP
                     MVRGPHGFGTGFERFSEARNVRALTLEVLREKFDRLKASRGVNLRRRVQRWKELVRWW
                     RATEDNGAARAANTVREWIAQRDANTPFFWFVNLIETHNPYFPPKPYNGFSGAERWRA
                     MEDVFRYESYLEVILTCKGDLDIPEDALLRMRHLYARSVQYLDGWIRGVFEELDGHGL
                     LDDTLVMITSDHGESFYENHLVNHGWSMDQRQLHVPFVAAGPGAPPARPLASLAEVPR
                     MIAEAAGIEDHPFIEDPLPGGVAVAQKDVTFQGRDEILERARLKGVPEGDLLRFMART
                     MACATDGSFKLSRIGEREALYDLARDPLEATDVSTEHPDRVRSLRAALDATGEAPQPV
                     TVPAGDGGEIAAEEEAEIEAQLEGLGYI"
BASE COUNT          277 a          737 c          720 g          268 t
ORIGIN      
        1 cgagacgagc accacctcga tgtgcccttg ccagcggaac acgtcgccga ccgcctgcca
       61 gcgcgccgca gggccgaggt cgttccacgg ccgcggcggg tagtacggcc ggtgggtctc
      121 catcaggttg acgaacgcga agaacggctt cgctccgccg gaatggtcga gccaccggcg
      181 cagggccctc ccggcgtgga ccgcgccgtt gtcgtcgcgg gcgcgccacc aggcggcccg
      241 gtcgcgggcg cgccgcgcgg cgccggccgg gccgcgccgc gccgccgccc gctggcgctg
      301 gcgctgcagg cgcgtgaacc gggtccggcc gggatcgccg aaccagtcga agcccgtcga
      361 gaacccggtc gcggagttca cgatcgggtt cgtgctcacc gcgccggtgg cgtacccgcc
      421 gtgtcgcagc acctccgcca gcgtgcggtc gcggtgccgg tcgaacagcg accgcatccg
      481 cgtgcgaagc tcccggggag cccagagacc gggcgtgaac ccgaacgcgc gcggcagcag
      541 gccggtgaac atcgaggcgt gtgaggggac cgtccacgac gacggcgcga ccgccatcgg
      601 aaacgcggtc ccgcggcggg ccagttcggc gaccaccggc gtcgacccgg ccggcgcgcc
      661 ccacggttcg aacgcgtcgg cgcgggcggt gtccatcagc accagcagga cgttcggacg
      721 gtcggccatc gcggctatca gatgtagccg aggccctcga gctgggcctc gatctcggcc
      781 tcctcctcgg ccgcgatctc gccaccgtcg ccggccggga cggtgaccgg ctgcggcgcc
      841 tcgccggtgg cgtcgagcgc ggctcgcagc gaccgaaccc gatcggggtg ctcggtcgag
      901 acgtccgtcg cctcgagcgg gtcgcgcgcc aggtcgtaca atgcctcgcg ctcgccgatc
      961 cgcgacagct tgaacgaccc gtcggtggcg caggccatgg tccgcgccat gaaccgcagc
     1021 aggtcgccct cgggcacgcc cttcagacgg gcccgctcca ggatctcgtc gcggccctgg
     1081 aacgtgacgt ccttctgcgc gacggcgacg ccgccgggca gcgggtcctc gatgaacggg
     1141 tggtcctcga tcccggcggc ctcggcgatc atccgcggga cctcggccag cgaggcgagc
     1201 ggccgcgccg gcggcgctcc cgggcccgcg gcgacgaatg gcacgtgcag ctgtcgctgg
     1261 tccatcgacc agccgtggtt caccaggtgg ttctcgtaga acgactcgcc gtggtccgag
     1321 gtgatcatga ccagcgtgtc gtcgagcagg ccgtgtccgt cgagctcctc gaagaccccg
     1381 cggatccagc cgtcgaggta ctggaccgag cgcgcataga gatggcgcat ccgcaacagg
     1441 gcgtcctcgg ggatgtcgag gtcgcccttg caggtcagga tgacctccag gtagctctcg
     1501 tagcgaaaca catcctccat cgcccgccac cgctcggcgc ccgagaagcc gttgtacggc
     1561 ttcggcggga agtacgggtt gtgggtctcg atcaggttca cgaaccagaa gaacggggtg
     1621 ttggcgtcac gctgggcgat ccactcgcgg acggtgttcg ccgcccgggc cgcgccgttg
     1681 tcctcggtgg cgcgccacca gcgcaccagc tccttccatc gctgtacccg gcggcgcagg
     1741 ttcacgccgc gggaggcctt caggcggtcg aacttctcgc gcagcacctc gagcgtcagg
     1801 gcgcgcacgt tgcgcgcctc gctgaagcgc tcgaacccgg tcccgaaccc gtgcggcccg
     1861 cgaaccatcg ggttcgtcga gagcgcccgt gtgccgtacc ccgcccgccg cagcacctcg
     1921 gccagcgtgc ggtcggcatg ccgggccatc accgccggca caacggtgcg caggtcgggg
     1981 accgtccaga gcgccggggc ga
//
LOCUS       DASPWO010000360         6395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1153795, whole genome shotgun sequence.
ACCESSION   DASPWO010000360 DASPWO010000000
VERSION     DASPWO010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153795"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VEO00_08275"
     CDS             <1..215
                     /locus_tag="VEO00_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58031.1"
                     /translation="EEVAIVAETALAGEALERVRREVVRRLWETAGVRAARVTLVPPG
                     TLPRTGSGKLSRAAVRRSIAGGMLDG"
     gene            complement(241..1344)
                     /locus_tag="VEO00_08280"
     CDS             complement(241..1344)
                     /locus_tag="VEO00_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU58032.1"
                     /translation="MLSVEGLRVAYPGRRGHAVLAVAGVSFEVERGHLFTLLGPSGCG
                     KTTTLRAIAGLERPQAGEIRLGDQVLYSGSNGVFVPPSDRHLGMVFQSYAIWPHMDVF
                     GNVAFPLVSAPRRRRPGRAAVRERVERALAAVRLDGLAGRPATDLSGGQQQRLALARA
                     LVTEPPLLLLDEPLSNLDAKLREEMRLEVKRLQRELGVTAVYVTHDQIEALTMSKYVA
                     VMHEGRIHQIGRPRDVYERPGTRFVAEFIGASNALPAKVESVGGEGHVLATDAGRLVV
                     RADPVPRVGSEVVVAIRPEEIELSVGASSEAPNRWPGTVLQRAFRGDAMDHVVDVRGV
                     ELLVRGHPRLTLPAGTAVTLELPPDACRLLPDE"
     gene            complement(1354..3024)
                     /locus_tag="VEO00_08285"
     CDS             complement(1354..3024)
                     /locus_tag="VEO00_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYU58033.1"
                     /translation="MVVGYLAIVPLGYLLWGTFFDAGGPTLRHFREAYASQGLGAMAA
                     NSLLFAAGSTVVAVGIGTVLAYLIARTDVPLKGLMFVAALVPLVIPGILHTIAWIFLA
                     SPRVGLINRALEPLLGPGAFDVFSMGGMIVVEGLHLSPLVFLLLYAAFRSMDPALEES
                     GLLGGARLRTVVRRVTLPLAAPALAAAVLVAVVRGLEAFEVPALLGIPGGVWVFTSRI
                     WRALATYPRRFGEAGAYAFSLLVLTAAGVLLYARLASRARRFQTITGRGFRPRPMPLG
                     RWRGPATAVLLAYVVVAVVLPLGVLLYASTQRFYTVPSFSAIAAGTLDNYRTLLGSGE
                     VLRAFRNSVALGIGSATVVTLLLAIVAWVVVRSRARARWALDAVAFLPLAVPGLVLGV
                     GLLFVYLRAPVPVYGTLWILFIAYVTRYMPYGMRYASAAMHQVGAELEEAAVVAGARW
                     WATFRRVVLPLLAPGLVAGWIYVLLVSFRELSGSILLYSPGNEVLSVVMWEQWQNGQA
                     TQLAALGVLMVAALALLAAAARRLGARIGIREPVAVAEAEEVLIGAER"
     gene            complement(3078..4214)
                     /locus_tag="VEO00_08290"
     CDS             complement(3078..4214)
                     /locus_tag="VEO00_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYU58034.1"
                     /translation="MTRRSLRLAAILLVLAACGSPPGSDREPARGLGAATRQTGRALE
                     RISRELDGLDPEARRARLIELAEEEGGTVNAYGSTNLDDMGALIDAFEDATGITVNYY
                     RANSEDVLQRLVEEADAGFRGADLVNTNGPELTLLDREGLLAEVDTPVTADLPPEGDL
                     GTWFWFYINTFTAAWNTDRVSAAEAPTSWEEVLSDHAGGLAFEAGDVDWFATLVKGYF
                     MAEKGMREEEAVALFTDAAAGARAVDGHTLMAELLTAGEFDVAASAYLHRIVRLQRDG
                     APVTWEPAVEPLLVRPSGAAVHAAAEHPAAAMLLLEFLLTDAQPMLLDFDRQPVNETV
                     EGGGLPPGVETIDVDVEAIVNEFDKWSDLYEQVIMATGGEVIED"
     gene            4288..5316
                     /locus_tag="VEO00_08295"
     CDS             4288..5316
                     /locus_tag="VEO00_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYU58035.1"
                     /translation="MHHREERVFVREVTARSYPLREFRRAMLDAPRVRGPEVVTEGSL
                     HRGDADVGHSGEAPTSRTWYRLSPGDEPFWTQTLQVHFVEIAPGGANRGHGHQNEAAF
                     YVLEGRGYEIHDEERYEWERGDVAIVHSDCVHRHFNASDTERALLLVVKAKPLWMFLG
                     LVQQGRQARMEEDSRWGPRVDWSDLWTPGVRGRRKVVKGADVPWTRARDGEIKVLSSA
                     AVPDLRLFSLDIYERRVPPGSRTGRHWHMADEVVYILAGRGHSLQWEVEAEIDGRYHA
                     RIAREPSRHDVRAGDLLYVPPNTVHQTVNDSPQEPLLLLAAQNRVVKMLGYDNVVHLE
                     DAPEWSGR"
     gene            5313..6188
                     /locus_tag="VEO00_08300"
     CDS             5313..6188
                     /locus_tag="VEO00_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58036.1"
                     /translation="MRAAADVRAALEARQRRAEPGLIEETGTIEVEGLRLFFHLWRPD
                     GEARRVGVSIAHSFVDHAILQAAEAAFGRAAARAGIAVILVQAPGAGDSEGAPEACTM
                     ADRIRAVAAGWAELRRRVPETERPCFLGAALGGLVAAHAAAGNDQACLALWQPSLHGD
                     AYWADVRRMLQLAMVRERRGEPRAADGDGPAPDPDAVLDREGLADVLGMRVTAAQLQE
                     LRDAGRGLDDGTVAGPALLVTAADRDERAAVRRIVRGPVEVAVGPGRRTAWHLGRPGG
                     LDVAGPTVDWLLRVG"
     gene            6196..>6395
                     /locus_tag="VEO00_08305"
     CDS             6196..>6395
                     /locus_tag="VEO00_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58037.1"
                     /translation="MEIPFFLPTSGGDLSASASLPGGGALAGRPTLLLTGGWHGRART
                     VLTLPLARRLAARGVPALRLDYP"
BASE COUNT          915 a         2391 c         2347 g          742 t
ORIGIN      
        1 ccgaggaggt cgccatcgtc gccgagaccg ccctggcagg cgaggcgctc gaacgggtgc
       61 gccgcgaagt cgttcgccgg ctgtgggaga cggcgggagt gcgcgcggcg cgggtgaccc
      121 tcgtcccgcc ggggacgctc ccccgcaccg gcagcgggaa gctcagccgc gccgcggtcc
      181 gccggtcgat cgccggcggg atgctcgacg gatagcccgc cgggcctcca ctccgtctcc
      241 tcactcgtcg ggcaggaggc ggcaggcgtc ggggggaagc tcgagggtca ccgcggtccc
      301 ggcgggcagg gtgagcctcg ggtggccgcg caccaggagc tccacgccgc ggacgtcgac
      361 cacatggtcc atggcgtctc cgcgaaaggc gcgctgcaac acggtgcccg gccagcggtt
      421 cggcgcctcg ctcgacgcgc cgaccgagag ctcgatctcc tcgggccgga tggcgaccac
      481 gacctccgag cccaccctcg gtaccggatc ggccctgacc accagacgac cggcgtcggt
      541 ggccagcacg tggccctcgc cgccgacgga ctcgaccttt gccggcagcg cgttcgaggc
      601 tccgatgaac tcggcgacga accgtgtgcc cggacgctcg tagacgtcgc gcgggcggcc
      661 gatctggtgg atgcgaccct cgtgcatgac cgcgacgtac ttggacatcg tcagcgcctc
      721 gatctggtcg tgcgtgacgt acaccgcggt cacgccgagc tcgcgctgca gccgcttcac
      781 ctccaggcgc atctcctcgc gcagcttagc gtcgaggttc gacagcggct cgtcgagcag
      841 cagcagcggc ggctcggtga cgagcgcccg ggccagcgcc aggcgttgct gctggccgcc
      901 ggagaggtcg gtggccggcc ggccggccag gccgtcgagg cggacggcgg cgagcgcccg
      961 ctccacccgc tcgcgcacgg cggcgcggcc cggccggcgc cggcgcggcg ccgacaccag
     1021 cgggaacgca acgttgccga acacgtccat gtgcggccag atcgcgtagg actggaagac
     1081 catccccagg tgccggtcgc tcggcgggac gaagactccg ttcgacccgg agtacagcac
     1141 ctggtcgccg aggcggatct cgccggcctg cgggcgctcc aggccggcga tcgcgcgcag
     1201 cgtggtggtc ttcccgcagc cggacgggcc gagcagcgtg aacaggtggc cgcgctcgac
     1261 ctcgaacgac accccggcga ccgccaggac ggcatggccg cgccggccgg gataggcgac
     1321 ccgcaggccc tcgacgctca gcaccgcggc tcgctagcgc tctgcaccga tcagcacctc
     1381 ctcggcctcg gccaccgcga cgggctcgcg gatcccgatc cgcgccccca ggcgccgcgc
     1441 cgccgccgcc agcagcgcga gggccgcgac catcagcacc ccgagcgccg ccagctgtgt
     1501 ggcctgcccg ttctgccact gttcccacat cacgaccgag agcacctcgt tcccgggcga
     1561 gtagagcagg atcgagccgg acagttctcg gaacgacacg agcagcacgt agatccaccc
     1621 cgcgaccagg cccggcgcga ggagcggcag caccacccgc cggaacgtcg cccaccaccg
     1681 cgcgccggcg acgacggccg cctcctcgag ctccgccccg acctggtgca tcgccgcgct
     1741 cgcgtagcgc atcccgtagg gcatgtaccg ggtcacgtag gcgatgaaca ggatccagag
     1801 cgtcccgtag accgggaccg gagcgcgcag gtagacgaac agcagcccga cgccgagcac
     1861 gagcccgggg acggcgagcg gcaggaacgc caccgcgtcg agcgcccagc gagcgcgggc
     1921 ccgcgagcgc acgacgaccc aggcgacgat cgccagcagc agcgtgacga ccgtggccga
     1981 cccgataccg agggccaccg agttgcggaa cgcgcgcagc acctcgccgc tcccgagcag
     2041 cgtccggtag ttgtcgagcg tgcccgccgc gatggccgag aacgacggga ccgtgtagaa
     2101 gcgctgggtc gaggcgtaga gcaggacccc gagcggcagg accaccgcga cgacgacgta
     2161 ggcgagcagg acggcggtgg ccgggccccg ccaccgcccg agcggcatcg ggcgcggacg
     2221 gaagccgcgt ccggtgatcg tctggaagcg ccgggcgcgc gacgccagcc gcgcgtacag
     2281 cagcacgccg gccgccgtca gcacgagcag cgagaacgcg tacgcgccgg cctcgccgaa
     2341 gcggcgcggg taggtggcga gcgcccgcca gatgcgggac gtgaacaccc agacgccccc
     2401 ggggatcccg aggagcgccg gcacttcgaa ggcctccagg ccccgcacca ccgcgaccag
     2461 gaccgccgcg gcgagcgccg gcgcggcgag cggcagcgtc acccggcgca cgacggtgcg
     2521 cagacgggcg ccgccgagca ggcccgactc ctcgagcgcc gggtccatcg agcggaatgc
     2581 cgcgtagagc agcaggaaga cgaggggcga caggtgcagg ccctccacca cgatcatccc
     2641 gcccatcgag aacacgtcga aggctcccgg gccgaggagc ggctcgaggg cccggttgat
     2701 caggcccacg cgcgggctcg ccaggaagat ccaggcgatc gtgtgcagga tccccgggat
     2761 cacgagcggc acgagcgccg ccacgaacat cagtcccttc agcgggacgt cggtccgcgc
     2821 gatcaggtag gcgaggaccg tgccgatgcc caccgcgacg accgtcgaac ccgccgcgaa
     2881 cagcagcgag ttggcggcca tcgccccgag cccctggctc gcgtaggcct cccggaagtg
     2941 gcgcagggtc gggccgccgg catcgaagaa cgtcccccac agcaggtacc cgagcggcac
     3001 gatcgccagg tacccgacga ccacgaccgc ggccccgacg atccaggtct gcggacgccg
     3061 gatcgccgcg agcgggctca gtcctcgatc acctcgccgc cggtggccat gatcacctgc
     3121 tcgtagaggt cggaccactt gtcgaactcg ttgacgatcg cctcgacgtc cacgtcgatc
     3181 gtctcgacgc cgggcggcag gccgccgccc tcgaccgtct cgttgaccgg ctggcggtcg
     3241 aagtcgagca gcatcggctg ggcgtccgtg agcaggaact cgagcagcag catcgccgca
     3301 gcggggtgct cggcggcggc gtggacggcg gcgccgctcg gccggaccag caggggctcg
     3361 accgccggct cccaggtcac cggcgcgccg tccctctgca gccgaacgat gcgatggagg
     3421 tacgccgagg ccgccacgtc gaactcgccc gccgtcagca gctcggccat cagcgtgtgg
     3481 ccgtcgaccg cgcgggcgcc cgccgccgcg tcggtgaaca gggccaccgc ctcctcctct
     3541 ctcatcccct tctcggccat gaagtagccc tttaccagag tggcgaacca gtcgacgtcg
     3601 ccggcctcga acgccaggcc gccggcgtgg tcggagagga cctcctccca gctggtcggc
     3661 gcctcggcgg cggacacccg gtccgtgttc caggccgcgg tgaatgtgtt gatgtagaac
     3721 cagaaccacg tgccgaggtc gccctccggc gggagatcgg ccgtcaccgg ggtgtccacc
     3781 tccgccagta gcccctcgcg gtccaggagg gtcagctcgg ggccgttcgt gttgacgagg
     3841 tcggccccgc ggaacccggc gtcggcctcc tcgaccaggc gctgcagcac gtcctccgag
     3901 ttcgcgcggt agtagttcac ggtgatcccc gtggcgtcct cgaaggcgtc gatcagcgcg
     3961 cccatgtcgt cgaggttcgt cgagccgtag gcgttgacgg tgccgccctc ctcctcggcc
     4021 agctcgatca ggcgggcccg gcgggcctcg gggtcgaggc catcgagctc gcgagagatc
     4081 cgctcgaggg cgcgacccgt ctggcgcgtg gcggcgccga gcccgcgcgc cggctcgcgg
     4141 tccgatcccg gcggtgagcc gcacgcggcc agcacgagca ggatcgccgc gaggcgaagg
     4201 gagcggcggg tcacgatccg cgcaccctat caactcgccc ccctccgacg cccgttccgc
     4261 gtcgggcgaa tctgggagga ttccgccgtg caccaccggg aggaacgcgt gttcgtacga
     4321 gaggtcaccg cgcggtcgta tccgctgcgc gagttccgcc gcgcgatgct cgacgcgccg
     4381 cgcgtccgcg gcccggaggt cgtcaccgag ggctccctgc accggggaga cgccgacgtg
     4441 ggccactccg gggaagcgcc cacctcgcgg acctggtacc gcctctcgcc gggcgacgag
     4501 cccttctgga cccagaccct gcaggtccac ttcgttgaga tcgcgccggg aggcgcgaat
     4561 cgcggccacg gccaccagaa cgaggccgcc ttctacgtcc tcgagggccg cggctacgag
     4621 atccacgacg aggaacgcta cgagtgggag cgcggcgacg tggccatcgt gcacagcgac
     4681 tgcgtccacc ggcacttcaa cgccagcgac accgagcgcg cgctgctgct ggtggtgaag
     4741 gccaagccgc tgtggatgtt cctcggcctg gtgcagcagg gacgccaggc ccgcatggag
     4801 gaggactcga ggtggggccc gcgcgtggac tggtcggacc tgtggacgcc cggcgtgcgc
     4861 gggcggcgca aggtggtgaa gggcgcggat gtcccgtgga cgcgggcccg cgacggcgag
     4921 atcaaggtcc tctcctccgc cgcggtgccc gacctgcgcc tgttcagcct cgacatctac
     4981 gagcggcgcg tgccgccggg gagccggacc ggccgccact ggcacatggc cgacgaggtc
     5041 gtctacatcc tcgctggtcg cggccacagc ctgcagtggg aggtggaggc ggagatcgac
     5101 ggccggtacc acgcccggat cgcccgcgag ccctcgcgcc acgacgtgcg ggccggagac
     5161 ctgctctacg tgccgccgaa caccgtccac cagaccgtga acgactcgcc ccaggaaccg
     5221 ctcctgctgc tggcggccca gaaccgcgtg gtcaagatgc tcgggtacga caacgtggtc
     5281 cacctggagg acgcaccgga gtggagcggc aggtgagggc ggcggcggac gtccgcgccg
     5341 ccctcgaggc tcggcagcgc cgggccgagc ccgggctgat cgaggagacc ggaacgatcg
     5401 aggtggaggg actgcgcctc ttcttccacc tctggcgccc ggacggcgag gcccggcggg
     5461 tcggcgtgtc gatcgcgcac tccttcgtgg accacgccat cctgcaggcc gccgaggcgg
     5521 cgttcggccg tgccgcggcc cgcgcgggga tcgccgtgat cctggtgcag gcgccgggcg
     5581 ccggggacag cgagggcgcc cccgaggcat gcacgatggc tgaccgaatc cgcgccgtcg
     5641 ccgccgggtg ggcggagctg cgccggcggg tcccggagac cgagcggccc tgcttcctcg
     5701 gggcggcgct cggcggcctg gtcgccgcac acgccgcggc gggcaacgac caggcctgcc
     5761 tggcgctgtg gcagccgagc ctgcacggcg acgcgtactg ggccgacgtc cggcgcatgc
     5821 tccagctggc catggtccgc gagcgccgcg gcgagccgcg ggcggcggat ggggacggcc
     5881 cggcccccga ccccgacgcg gtcctcgacc gggaggggct ggccgacgtg ctcggcatgc
     5941 gcgtgaccgc cgcgcagctc caggagctcc gggacgccgg gcgcgggctc gatgacggaa
     6001 cggtggccgg cccggccctc ctcgtcaccg cggccgatcg cgacgagcgg gcggcggtcc
     6061 gccggatcgt gcggggcccg gtcgaggtcg ccgtcggccc cgggcggcgc accgcctggc
     6121 acctcggccg ccccggaggg ctggacgtgg ccgggccgac ggtcgactgg ctgcttcggg
     6181 tgggctgagg acccgatgga gatccccttc ttcctgccca cgtccggcgg tgacctctcc
     6241 gcctcggcga gcctccccgg gggcggcgcc ctggccggcc gcccgacgct gctgctgacc
     6301 ggcggctggc acggccgggc gcggacggtg ctgacgctcc ccctcgcccg gcgcctggcg
     6361 gcgcgcggcg tcccggccct gcgcctggac tatcc
//
LOCUS       DASPWO010000361         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_564850, whole genome shotgun sequence.
ACCESSION   DASPWO010000361 DASPWO010000000
VERSION     DASPWO010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564850"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VEO00_08310"
     CDS             <1..964
                     /locus_tag="VEO00_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYU58038.1"
                     /translation="AVNDAAVSPAHLAHLSATELPIEVVAMLVGPRGKLAAPREAVDG
                     PVTGATVGHVRIGPLKLFADGAERCALRVRLPMLTKAARALATAKTGPGPMESLRLLR
                     PTVAPGGIRLGTLHYAPDDLRGLVAAATARGFGVAIHAVGNEGIRAALETLEPFGGAG
                     HRIEHAMFAEPRQAEAMARLGIGAAVQPGHLYPYGELVRAAGVEKDLPPVPLRRMEIE
                     GVSFAISSDAAGGFWEPLRTMAVAVTRQAGHGEPLAPAEAIDPEVALRAATVTAARVG
                     GILDAGQIAPGARANLAVLSGDPFDPGTRVVETWIDGRRVYPAA"
     gene            complement(981..1739)
                     /locus_tag="VEO00_08315"
     CDS             complement(981..1739)
                     /locus_tag="VEO00_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYU58039.1"
                     /translation="MTRFDVALDPPEAERLAGSDPRWWFTHMAFANAESPRHPAFRKL
                     EEITNLKRELLLPWIQKVVPGKSVLDTFCANGAFSFDAAEAVASRVVGVDFDPPRIHA
                     GNLVSELLRNHGWPVVPEFRVADIYRLEEVVTEPFEVTFCLGGLYHVADPIYVLRKVR
                     AATAGGGTLVMQTSGIIWKKGHHSVFQLRKQDRTGMGLTSIKAGAGVWTFTPAQVDRF
                     LECAGFEVLERATAPRPQRKEYPWYCTLARAVPA"
     gene            1813..>2022
                     /locus_tag="VEO00_08320"
     CDS             1813..>2022
                     /locus_tag="VEO00_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYU58040.1"
                     /translation="MQPNVLLIVLDTARADAFEPYGAVPGSSPVVADLARRGSAFPMM
                     VSTSCWTIPAHASLFTGLLPRAVGFA"
BASE COUNT          260 a          742 c          750 g          270 t
ORIGIN      
        1 ggcggtgaac gacgcggccg tctcccccgc ccacctggcg cacctctccg ccaccgagct
       61 gccgatcgag gtggtggcga tgctggtcgg gccgcgcggg aagctcgccg cgccgcgcga
      121 ggccgtcgac gggccggtga ccggcgccac cgtcggccac gtccggatcg gcccgctgaa
      181 gctgttcgcc gacggcgccg agcgctgcgc gctgcgggtg cgcctgccga tgctgacgaa
      241 ggcggctcgc gcactggcca ccgcgaagac ggggccgggc ccgatggagt ccctgcggtt
      301 gctccgcccg acggtcgctc ccggcgggat ccgtctcggg acgctgcact acgcccccga
      361 cgacctccgc ggcctggtgg ccgccgccac cgcgcgcggg ttcggcgtgg cgatccacgc
      421 ggtgggcaac gagggcatcc gcgcggccct cgagaccctc gagccgttcg gcggcgcggg
      481 gcaccgaatc gagcacgcca tgttcgccga accgcgccag gccgaggcga tggcccggct
      541 cgggatcgga gccgccgtcc agcccgggca cctgtacccg tacggggagc tggtccgggc
      601 ggccggagtg gagaaggacc tgccgccggt gccactgcgg cggatggaga tcgagggggt
      661 ctcgttcgcg atctcgagcg atgcggccgg cggcttctgg gagccgctgc ggacgatggc
      721 cgtcgcggtg acgcggcagg cgggccacgg cgagccgctc gctccggccg aggcgatcga
      781 ccccgaggtc gccctacgcg cggccaccgt caccgccgcc cgggtcgggg ggatcctgga
      841 tgccggccag atcgcccccg gcgcgcgcgc caacctcgcg gtcctgtccg gcgacccgtt
      901 cgacccggga acccgcgtgg tagagacctg gatcgacggc cgccgcgtct acccggcggc
      961 gtgagcgagc gaggacgagc tcaggccggg acggcgcggg ccagggtgca gtaccacggg
     1021 tattccttgc gctgcggccg tggcgcggtg gcgcgctcga ggacctcgaa gccggcgcat
     1081 tccaggaagc ggtcgacctg cgccggggtg aacgtccaga cgcccgcgcc ggccttgatc
     1141 gaggtcaggc ccatgccggt gcggtcctgc ttgcgcagct ggaacacgga gtggtggccc
     1201 ttcttccaga tgatgccgct cgtctgcatc acgagcgtcc cgcccccggc ggtcgcggcg
     1261 cgcaccttcc tcaacacgta gatcgggtcg gcgacgtggt agaggccgcc gagacagaac
     1321 gtgacctcga acggctcggt gacgacctcc tccagccggt agatgtcggc cacgcgaaac
     1381 tccgggacca ccggccagcc gtggttgcgc agcagctcgg agaccaggtt gccggcgtgg
     1441 atccgcggcg ggtcgaagtc gacgcccacg acgcgcgacg ccaccgcctc cgccgcgtcg
     1501 aacgagaacg ccccgttcgc gcagaacgtg tcgagcacgg acttccccgg aacgaccttc
     1561 tggatccagg gcagcagcag ctcccgcttg aggttcgtga tctcctcgag cttgcggaag
     1621 gcggggtgcc gggggctctc ggcgttggcg aaggccatgt gggtgaacca ccagcgcggg
     1681 tcgctgccgg cgagccgctc cgcctccggc ggatcgaggg cgacgtcgaa gcgcgtcatc
     1741 ggccggggat gctactgcac gcccaccgtc gccgaccggc cggtcccgga ggcatcgact
     1801 aggctccgtg cggtgcagcc gaacgtcctg ctgatcgtcc tcgacaccgc gcgcgccgac
     1861 gccttcgagc cgtacggcgc ggtaccagga tcgtcccccg tcgtcgccga cctggcccgg
     1921 cgcgggtcgg ccttcccgat gatggtctcg acctcgtgct ggacgatccc ggcgcacgcg
     1981 tcgctgttca ccggcctgct gccgcgcgcg gtgggcttcg cc
//
LOCUS       DASPWO010000362         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_983980, whole genome shotgun sequence.
ACCESSION   DASPWO010000362 DASPWO010000000
VERSION     DASPWO010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983980"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VEO00_08325"
     CDS             complement(<1..1362)
                     /locus_tag="VEO00_08325"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HYU58041.1"
                     /translation="MPALDGLRGLAIAGVLLFHADHLTGGYLGVDLFFVLSGFLITSL
                     LLGEWAADGRISLAGFWARRARRLLPALVGVLIGVAVYAAVWADARELGRIRGDALAT
                     LGYVANWRAIFSGNGYWDVFATPSPLEHTWSLAIEEQFYVVWPLAALAVLWARRGSAR
                     AVLTVALLLGGLSAAWMMLSYTPGADPERVYLGTDTRGAAILFGAALAAAYACWGPPA
                     RRVVRGALEVAGVLGAAALTWAWAGLDGRDDRLYRGGFLACAVAAVAVIAAAACPTPG
                     PLGRTLSVAPLRALGAISYGLYLWHWPVYVVLTPQRSGLDGWTLTGVRIAVSVAIAVV
                     SYALLELPIRRGALDAARLRVVAPSAAVLAMGALLVTTAGARSVPSVPAALSDRPTAD
                     APASPPGPGSAAPVGDFSVDWAALAAAAHVIPNTPPPVRGWRLLERHGKQLAGQPAAD
                     DDGA"
     gene            complement(1421..1882)
                     /locus_tag="VEO00_08330"
     CDS             complement(1421..1882)
                     /locus_tag="VEO00_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58042.1"
                     /translation="MTRPNSGTTVWCPSCGAEYVAGSRSCSDCRVGLVPDRPFLGDDP
                     PRSASGGDDALCELGEWPRLPAQILRRRLETAGITVMVEWSGPGADAIGTLVVPEAQA
                     EFAEAVVHEIDVDDEVPDTSPLAYLGRIEEHLAAAGALLDELRTRLEDGGT"
BASE COUNT          298 a          759 c          762 g          216 t
ORIGIN      
        1 ggcgccgtcg tcgtcagccg ctggctgacc cgccagctgc ttaccgtggc gctctagtaa
       61 ccgccatcca cgaacaggcg gcggggtgtt ggggatcacg tgggcggcgg cggcgagcgc
      121 ggcccagtcg accgagaagt cgccgaccgg cgccgccgat cccggtcccg gcggagacgc
      181 cggcgcgtcg gcggtgggtc ggtcggagag cgcggcgggc acggacggga cgctccgtgc
      241 gccggcggtg gtgacgagca gcgcgcccat ggcgaggacc gcggccgacg gcgccaccac
      301 ccggagccgg gccgcgtcga gcgcgccgcg gcggatcggc agctccagca gcgcgtacga
      361 cacgaccgcg atggcgacgc tcaccgcgat gcgcacgccc gtgagggtcc agccgtccag
      421 gccggaccgt tgcggcgtga gcacgacgta tacgggccag tgccacaggt agagcccgta
      481 gctgatcgcg cccagcgccc ggagcggggc gaccgacagc gtgcggccca gcgggccggg
      541 agtcgggcag gcggcggccg cgatcaccgc gaccgccgcc accgcgcagg cgaggaaccc
      601 gccgcgatag agccgatcgt cgcgcccgtc gaggccggcc catgcccacg tgagcgcggc
      661 ggctccgagc acgccggcga cctcgagcgc gccccgcacc acccggcgcg cgggaggacc
      721 ccaacacgcg taggcggctg cgagcgcggc gccgaagagg atggccgcac cgcgggtgtc
      781 ggtgccgagg tacacgcgct cgggatcggc gccgggcgtg tacgacagca tcatccaggc
      841 ggccgagagc ccgccgagga gcagtgcgac ggtgaggacc gcccgggccg agccgcggcg
      901 cgcccagagc accgcgagcg ccgccagcgg ccacaccacg tagaactgct cctcgatggc
      961 gaggctccac gtgtgctcca gtggtgacgg cgtcgcgaac acgtcccagt acccgttgcc
     1021 gctgaagatg gcgcgccagt tggccacgta gccgagcgtg gccagcgcgt cgccgcggat
     1081 gcgccccagc tcccgggcgt cggcccagac cgcggcgtac accgcgaccc cgatgagcac
     1141 tccgaccagc gcgggcagga gtcgccgcgc ccgccgggcc cagaaccccg cgagggagat
     1201 ccgcccgtcg gcggcccact cgcccaggag cagcgacgtg atcaggaagc cggagaggac
     1261 gaagaagagg tcgacgccga ggtagccgcc ggtcaggtgg tcggcgtgaa acagcaggac
     1321 cccggcgatc gcgaggcccc gcagcccgtc gagtgcgggc atgtgtcgaa gtggtctgcc
     1381 cattcccccc ggtggcgagc accctggcgg cgccgcccgt ctacgtcccg ccgtcttcca
     1441 gacgggtccg cagctcgtcg aggagcgccc ccgccgcggc gaggtgctcc tcgatgcgcc
     1501 caaggtaggc gaggggggac gtatcgggca cctcgtcgtc gacgtcgatc tcgtgcacga
     1561 cagcctcggc gaactcggcc tgggcctcgg gcacgacgag ggtcccgatc gcgtcggcgc
     1621 ccgggcctga ccattcgacc atcacggtga tgccggcggt ctccagtcgc cgccgcagga
     1681 tctgggccgg cagccggggc cactcgccga gctcgcagag ggcgtcgtca ccgccgctcg
     1741 ccgaccgggg cgggtcgtcg ccgaggaacg gccggtcggg caccagcccg actcggcagt
     1801 cgctgcacga ccgcgagccg gcgacgtact ccgctccgca cgacgggcac cagaccgtcg
     1861 tgccgctgtt cggccgggtc aagccacccc cctgacgctg acgctcgggt cagtctacgg
     1921 aggccgccgg taggcttcgg tcgtggccgc cccgcggagt ttccatgtcg agacgctcgg
     1981 ctgcccgaag aacgccgtcg actccgacaa ggtcacggcg tcgctcctcg ccgac
//
LOCUS       DASPWO010000363         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_820889, whole genome shotgun sequence.
ACCESSION   DASPWO010000363 DASPWO010000000
VERSION     DASPWO010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820889"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="VEO00_08335"
     CDS             <1..1340
                     /locus_tag="VEO00_08335"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132661.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HYU58043.1"
                     /translation="IAEHTGPFAGFAPNRDAMLRVTRKHRAAADAIDAELVPEGLLQS
                     AKTAWDEAIGFGEEHGYRNAQASVLAPTGTIGLMMDCDTTGIEPDLALVKTKKLVGGG
                     TMQIVNQTIPRALRRLGYDAAQAAAIVDYISEHNSVVGAPHLKAEHHPVFDTSMGEQA
                     IHYLGHVRMMGAVQPFISGAISKTVNMPEDVTVEEVEQLFVEGWRLGLKAIAIYRNNC
                     KVAQPLSADKKAKAAVAAAPEAEARPTRRRLPATRPAKTFSFTVGDADGYIIAGEYPD
                     DGIGEIFLKVAKQGSTLAGIMDAFAISVSLGLQYGVPLQAYVEKFVNMRFEPSGITSD
                     PDVRFAASLVDYIFRRLALEYLPGETRTNLGIQSIEERKNGIKGEANGASYAPASSSA
                     PVSSSEIPVADVTPDRPTSRAVDAPLCYVCGSKMQPSGSCYVCGSCGATSGCS"
     gene            1397..1780
                     /locus_tag="VEO00_08340"
     CDS             1397..1780
                     /locus_tag="VEO00_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018729619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU58044.1"
                     /translation="MTGFALTSLHHVQLAMPPGEEAKAEAFYAGVLGLSRVPKPPDLV
                     ARGGCWFRGPGVAVDLGVDEPFAPARTAHPAFLVRGLDALRERLVAAGAEVDAGQPPL
                     QGLRRIHAFDPFGNRLEFLEPDPPR"
     gene            1791..2180
                     /locus_tag="VEO00_08345"
     CDS             1791..2180
                     /locus_tag="VEO00_08345"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HYU58045.1"
                     /translation="MPRALLPLFAAGLLVACSGGPRELEVVVGEAATERTVRVAPEQA
                     FVVIVGTNASIGWTWDLTVEPDTAVAEFVDRRYVPDEPVQPGSGGVDRFRFRAVGAGQ
                     TSLRLSRDFRGEGPDRQLTITVVVRAS"
     gene            2187..2636
                     /gene="dut"
                     /locus_tag="VEO00_08350"
     CDS             2187..2636
                     /gene="dut"
                     /locus_tag="VEO00_08350"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HYU58046.1"
                     /translation="MEVPIRRLDPSAPLPGHARPGDAGLDLAANAEVEIGPGERALVP
                     TGLAIAIPEGHAGLVLPRSGLASKHGIGLANAPGLIDAGYRGEVTVSVINHDRSTPYR
                     IRRGERIAQLVIVAVPAVTLREVDELPDSERGGGGFGSTGGFGSGGP"
BASE COUNT          369 a          950 c          971 g          359 t
ORIGIN      
        1 ggatcgccga gcacaccgga ccgttcgccg gcttcgcccc gaatcgcgac gcgatgctcc
       61 gggtgacgcg caagcaccgg gccgccgcgg acgcgatcga cgccgagctc gtccccgagg
      121 gcctgctgca gtccgcgaag accgcgtggg acgaggccat cgggttcggc gaagagcacg
      181 gctaccgcaa cgcgcaggca tcggtgctcg cgccgaccgg gacgatcggc ttgatgatgg
      241 actgtgacac caccggcatc gagcccgacc tggcgctggt caagacgaag aagctggtcg
      301 gcggcgggac gatgcagatc gtcaaccaga cgatcccgcg ggcgttgcga cggctcggct
      361 acgacgcggc gcaggccgcc gcgatcgtcg actacatctc cgagcacaac tcggtggtcg
      421 gcgcgccgca cctgaaggcc gagcaccacc cggtcttcga cacctcgatg ggcgagcagg
      481 cgatccacta cctcggccac gtccggatga tgggcgcggt ccagccgttc atcagcgggg
      541 cgatctcgaa gaccgtcaac atgccggagg acgtcacggt cgaggaggtc gagcagctgt
      601 tcgtcgaggg gtggcgcctg gggctcaagg cgatcgccat ctaccggaac aactgcaagg
      661 tcgcgcagcc gctgtcggcg gacaagaagg cgaaggcggc cgtcgcggcg gcgcccgagg
      721 ccgaggctcg cccgacccgc cggcgcctcc cggcgacgcg gccggcgaag accttctcgt
      781 tcacggtcgg ggatgccgac ggctacatca tcgcgggcga gtaccccgac gacggcatcg
      841 gcgagatctt cctgaaggtc gccaagcagg gctcgacgct cgccgggatc atggacgcgt
      901 tcgcgatctc ggtctctctc ggcctgcagt acggcgtgcc gctgcaggcc tacgtcgaga
      961 agttcgtcaa catgcgcttc gagccgtcgg ggatcaccag cgacccggac gtgcggttcg
     1021 cggcgtcgct ggtggactac atcttccggc gcctggcgct cgagtacctg ccgggggaga
     1081 cccggacgaa cctcggcatc cagtcgatcg aggagcgcaa gaacgggatc aagggcgagg
     1141 cgaacggcgc ctcgtacgcg cccgcctcgt cctcggcgcc ggtgagctcg tcggagatcc
     1201 cggtggccga cgtcacaccg gaccggccga cctcgagggc ggtcgacgcg ccgctctgct
     1261 acgtctgcgg ctcgaagatg cagccctcgg gctcctgcta cgtctgcggc tcctgcggcg
     1321 ccacgagcgg ctgctcgtag cggccaccgg acccgagggg cgggcccccc gcccgcccct
     1381 cgcctccatc ggtcccatga ccgggttcgc gctcacgagc ctccaccacg ttcagctggc
     1441 gatgccgccc ggcgaagagg cgaaggccga ggccttctac gcgggcgtcc tcgggctgtc
     1501 tcgggtcccg aagccgccgg acctcgtggc ccgcggcggg tgctggttcc gcggtcctgg
     1561 cgtggcggtg gacctcggcg tcgacgagcc cttcgcgccg gcgcgcacgg cgcacccggc
     1621 cttcctggtg cgcggcctcg acgccctccg cgagcgcctc gtcgcggccg gcgccgaggt
     1681 ggacgccggc cagccaccgc tacagggact gcgacggatc cacgcgttcg acccgttcgg
     1741 aaaccgcctg gagttcctgg agcccgaccc gcctcgttag catgcttgtc atgccacggg
     1801 cgctcctccc cctgttcgcc gcgggcctgc ttgtggcgtg ctcgggcggg ccgcgggagc
     1861 tggaggtcgt ggtcggggag gctgcgaccg agcgaacggt gcgggtcgcg cccgagcagg
     1921 cgttcgtcgt gatcgtgggg acgaacgcct cgatcggatg gacctgggac ctgacggtcg
     1981 agcccgacac ggccgtggcc gagttcgtcg accgccgcta cgtcccggac gagcccgtgc
     2041 agccgggcag cggcggcgtg gaccgcttcc gcttccgggc cgtgggggcg gggcagacca
     2101 gcctgcggct cagccgcgac ttccggggtg agggccccga tcgccagctc acgatcacgg
     2161 tcgtggtccg ggcgagctga aagccggtgg aggtcccgat ccgccggctc gacccgtcgg
     2221 ccccgctccc cgggcacgcc cgacccggcg acgccgggct cgacctggcc gcgaacgccg
     2281 aggtggagat cgggcccggg gagcgcgccc tggtcccgac cggcctggcc atcgcgatcc
     2341 ccgagggcca cgccggcctg gtccttccgc gctcgggact ggcctcgaag cacgggatcg
     2401 ggctggcgaa cgcgcccggg ctgatcgacg ccggctaccg cggggaggtc acggtgtcgg
     2461 tgatcaacca cgaccggtcg accccctacc gcatccgccg cggtgagcgc atcgcccaac
     2521 tggtgatcgt ggcggtgccc gcggtgaccc tgcgcgaggt ggacgagctc cccgacagcg
     2581 agcgcggcgg gggcggcttc ggctcgaccg gcgggttcgg gtcgggcggc ccctgaggta
     2641 gactggaag
//
LOCUS       DASPWO010000364         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1073979, whole genome shotgun sequence.
ACCESSION   DASPWO010000364 DASPWO010000000
VERSION     DASPWO010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073979"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..780
                     /locus_tag="VEO00_08355"
     CDS             235..780
                     /locus_tag="VEO00_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU58047.1"
                     /translation="MARLHARGIHEGFLATLGEPFLTLLYEAMASSDRAVLLVARTGT
                     GAVAGFVAGAAHPGAFYREFLLRRGVRAAARLAPRAVRPQVARRIWETLRHLGAEGGG
                     GPELLSIAVGDRGRRSGTGTALARGLEDALGAGGASRLVVVVAERNAPARAFYERLGF
                     RPARTLEVHRGERSIRYEKPL"
     gene            849..2039
                     /locus_tag="VEO00_08360"
     CDS             849..2039
                     /locus_tag="VEO00_08360"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYU58048.1"
                     /translation="MSEDPIPLARPDLGPDEEAAVIDVLRSGQLALGPRAEEFERLVA
                     EYCGSPWAVSTSSGTAALHLAVRALGIGPGDCVATVSFSFIASANALVYEGADPVFLD
                     IEPGTNGMDPAALKAYLEACSPGGDGRPRDPVTGRRVAAVLPVHAFGHPADADAIGAL
                     AAERGLPVIEDACEALGSWYRGADGAWRHAGTAGAFGAFAFYPNKTITTGEGGMLVGH
                     DAALGELARSLHNQGRAPDAAWLHHDHLGFNYRLDELSAALGVVQMRRLPEILERRAR
                     VAAWYGEALAAVPEATPPHGAEWARPAWFVYTVRLDQAVDRDAVVAALAAEGIASKAY
                     FEPPIHRQPPYAGRPDLIPFALSETDRAARDTLVLPYFTGMTQGQVGRVAETLGKVVT
                     VCRR"
     gene            2027..>2337
                     /locus_tag="VEO00_08365"
     CDS             2027..>2337
                     /locus_tag="VEO00_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58049.1"
                     /translation="MPTVGRARRVLGHVRVTGRKRLAFFVLADAAVFTVSLYGAFTLR
                     FELDVPRVYLHSIPYLLALVVVVRLAVNALFRLYSLTWRFVGTRDMLNVVLATILGTV
                     I"
BASE COUNT          254 a          897 c          869 g          317 t
ORIGIN      
        1 gacgtacccg agcccgacca cggcgacggg tgtctcccgg cggcggatgc gttccttgag
       61 cggccgccac cagccgggag tgacgggggt gggtttcacg gcctgcacga gaggtggcat
      121 cgtaggcgca gccgagcggg agggtcaacg acgagcgcga agacgaagcg atcggccggt
      181 gagcggccgg cggcgccgcg ggtcgtcccg gcgcgcccgg ccgaggccgc cgcggtggcc
      241 cggctgcacg cccgcggcat ccacgagggc ttcctggcca ccctggggga accgttcctg
      301 accctgctct acgaggcgat ggcgtcctcg gaccgggccg tgctcctcgt cgcccgcact
      361 gggaccggcg ccgtcgccgg gttcgtggcc ggcgccgccc accccggcgc cttctaccgg
      421 gagttcctgc tccgtcgcgg cgtgcgggcg gcggcccgcc tcgcccccag agcggtgcgg
      481 ccgcaggtcg cccgccggat ctgggagacg ctgcgccacc tcggcgccga gggcgggggc
      541 ggccccgagc tgctctcgat cgcggtcggt gatcgcggcc ggcgttccgg gaccggcacg
      601 gctctggccc gcggcctgga ggacgccctg ggcgccggcg gggcctcccg cctggtcgtg
      661 gtggtggccg agcgcaacgc ccccgcccgg gccttctacg agcgcctcgg cttccgcccg
      721 gcccgcaccc tcgaggtcca ccgtggcgag cgctccatcc gctacgagaa gccgctctga
      781 cgcggtcctc tgggaacggc ccggcggggc ggatatgctg tccgcggccg gcgaccggcc
      841 tgtcgatcgt gagcgaagac cccatccccc tggcgcggcc cgacctcggc cccgacgagg
      901 aggccgcggt gatcgacgtc ctgcgcagcg gtcagctcgc gctcggcccg cgtgccgagg
      961 agttcgagcg cctggtggcc gagtactgcg ggtcgccctg ggccgtgtcg acctcgagcg
     1021 ggaccgccgc gctgcacctc gcggtgcgcg cgctcgggat cgggccgggg gactgcgtgg
     1081 cgacggtgtc gttctcgttc atcgcctcgg cgaacgcgct ggtctacgag ggcgccgatc
     1141 ccgtgttcct ggacatcgag cccgggacga acggcatgga cccggccgcg ctgaaggcct
     1201 acctggaggc ctgttcgccg ggtggcgacg ggcggccgcg cgacccggtc accgggcggc
     1261 gggtggcggc cgtgctcccg gtgcacgcgt tcgggcaccc cgccgacgcc gacgccatcg
     1321 gagccctcgc cgccgagcgc ggcctgccgg tgatcgagga cgcctgcgag gccctcgggt
     1381 cgtggtaccg gggcgccgac ggggcgtggc gccacgccgg caccgccggc gcgttcggcg
     1441 cgttcgcgtt ctacccgaac aagaccatca ccaccggcga gggcggcatg ctggtcggcc
     1501 acgacgccgc cctcggcgaa ctggcccgca gcctgcacaa ccagggccgc gcgcccgacg
     1561 ccgcctggct ccaccacgac cacctcggct tcaactaccg cctcgacgag ctgtcggcgg
     1621 cgctcggcgt cgtccagatg cggcgcctgc cggagatcct cgagcgccgc gcccgcgtgg
     1681 cggcctggta cggcgaggcg ctggccgccg tcccggaggc gaccccgccg cacggcgcgg
     1741 agtgggcgcg gcccgcctgg ttcgtctaca cggtccgcct ggaccaggcc gtcgaccgcg
     1801 acgccgtggt cgccgcgctg gcggccgagg gcatcgcctc gaaggcgtac ttcgagccgc
     1861 cgatccaccg ccagcccccg tatgccggcc gcccggacct gatacccttc gcgctctccg
     1921 agaccgaccg ggccgcccgc gacaccctcg tcctgccgta cttcacgggg atgacgcagg
     1981 gccaggtcgg ccgggtggcc gagacgctgg ggaaggtggt gacggtgtgc cgacggtagg
     2041 ccgcgcgcgg cgggtgctcg ggcacgtccg ggtcaccggc cgcaagcgac tggccttctt
     2101 cgtcctcgcc gacgccgccg tcttcaccgt ctcgctgtac ggggccttca ccctgcgctt
     2161 cgagctcgac gtcccgcgcg tctacctgca ctcgatcccc tacctcctgg cgctcgtcgt
     2221 ggtcgtccgg ctggcggtga acgcgctctt ccgcctgtac agcctgacct ggcgcttcgt
     2281 cgggacccgg gacatgctga acgtcgtcct ggcgacgatc ctcgggacgg tgatctg
//
LOCUS       DASPWO010000365         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_134810, whole genome shotgun sequence.
ACCESSION   DASPWO010000365 DASPWO010000000
VERSION     DASPWO010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134810"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1780
                     /locus_tag="VEO00_08370"
     CDS             143..1780
                     /locus_tag="VEO00_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYU58050.1"
                     /translation="MDKPRHGGHLVAQALANEGVTHLFTLSGGHIAPIYDGCVDLGIR
                     IVDHRHEQAAAHAADGWARVTGKTGVAAVTAGPGLTDAVTGIANAMYANAPVLVLSGK
                     NPIIEFEMGSLQEMDQVTLVSSITKWAKTCYETRKLGDYVAAAFRHANAGRKGPVFLD
                     IPLDVQFNAWPPGVDVPTGYRTEARPLGDPDEVAGAVRLLAEAEHPIVFAGAGVRWSE
                     ANVELTALADALKAPVFLNSLGRGCLSPDHPYAFSAARRYALGRADVILALGVDWDFR
                     LGFGQRGFREDVKVIQVDVDATHVGRNRPVEVGIVGDPGKVIEQLLDAGAGTADESSW
                     TRDVRDEEQRLQGEAEAGMNADAVPIHPQRFAREIRDFLDRDAIVVGDGGDIVGISAS
                     IIQAHAPGHWLDTGPFGCLGVGPSFAIAAKLARPDKQVAIVYGDGSFGLNAMEYESAI
                     RQDIPFVGIIGNDGAWGQIKVAQEAMYGQGNAPAAGLSTATPYHKMVEGLGGYGELVT
                     EPGELRPALQRAFDAGVPACLNVVIDPTLMKRASYLG"
     gene            1783..2205
                     /locus_tag="VEO00_08375"
     CDS             1783..2205
                     /locus_tag="VEO00_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HYU58051.1"
                     /translation="MRADNFPDPDRPRRRFGLFRSRAEKKGKQGKGRGRAAKPDAGTV
                     KSIEEFIATRVGVEAYLEPETPTNPLSVVFVAADGEWIRKRIPDAAWLSKVSKAARMP
                     VYEVARVGYPRRMREYRRRPPGSPDAPGPTDLPDLPEP"
     gene            2267..3022
                     /locus_tag="VEO00_08380"
     CDS             2267..3022
                     /locus_tag="VEO00_08380"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HYU58052.1"
                     /translation="MAGGVVEVARRVAGVQAQVMSNAELAIGIRARGVTPAKVRAVQR
                     QRSLVKTWAFRGTIHLLPADELPMWIAALGTWTGYRRGAWLKWFRVTAEELDAVLEGI
                     GRALDGRNLTREQLAREVARVTKRRHLEEIVKGSWGSALKPATRQGLLIFGPVEGRNV
                     TFVRPDQWVKGWHTADPEASMDEAIRRYLDAYGPATHEDFARWFGLDLAPSRKRFLRL
                     AEELVEVEVDGDRAWMGCRASPAGSPRSSWSTA"
     gene            3733..>4019
                     /locus_tag="VEO00_08385"
     CDS             3733..>4019
                     /locus_tag="VEO00_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HYU58053.1"
                     /translation="MVGYWRQQADGRHPERRFERRGLHVSPTVFGMVRVDGDLDPENG
                     QALITAIRAVVDAETRSGDESDTRTPSQRRADALGEICRRHLACADRPLVGG"
BASE COUNT          570 a         1374 c         1517 g          558 t
ORIGIN      
        1 cgagtggaga cgcggacgaa ccctgccatc tcggcccgag tgtaggggcg acgcttccgg
       61 ccgcctaggg gtcgccggaa gtcaggcgtc gtcgacggcg ccgcgttgag cgcctacgat
      121 ctccggacga ttgaggagga cgatggacaa gccccgccac ggaggacatc tcgttgccca
      181 ggcccttgcc aacgagggcg tgacccacct gttcacgctg tccggcggcc atatcgcgcc
      241 gatctacgac ggctgcgtgg acctcggcat ccgcatcgtc gaccaccgcc acgagcaagc
      301 cgccgcccac gccgccgacg ggtgggcgcg ggtcaccggg aagaccggcg tggccgcggt
      361 caccgcgggc ccgggcctta ccgacgcggt caccggcatc gccaacgcga tgtacgcgaa
      421 cgccccggtg ctggtcttga gcggcaagaa cccgatcatc gagttcgaga tgggttcgct
      481 gcaggagatg gaccaggtca cgctcgtctc ctcgatcacg aagtgggcga agacctgcta
      541 cgagacgcgc aagctcgggg actacgtcgc cgccgcgttc cggcacgcga acgccgggcg
      601 caagggcccg gtgttcctcg acatcccgct cgacgtgcag ttcaacgcct ggccgccggg
      661 ggtggacgtg cccaccgggt accgcaccga ggcccggccg ctcggcgatc ccgacgaggt
      721 ggccggcgcg gtgcgtctgc tggcggaggc ggagcacccg atcgtgttcg ccggcgccgg
      781 cgtccgctgg tcggaggcca acgtcgagct gaccgcgctg gccgatgcgc tgaaggcgcc
      841 ggtgttcctg aactcgctcg ggcgggggtg cctgtcgccc gaccacccgt acgcgttctc
      901 ggccgcgcgc cgctatgcgc tcggccgggc cgacgtgatc ctggcgctcg gcgtggactg
      961 ggacttccgg ctcggcttcg ggcagcgcgg gttccgcgaa gacgtgaagg tgatccaggt
     1021 cgacgtcgac gccacccacg tcggacgcaa ccgcccggtc gaggtcggga tcgtcggcga
     1081 cccgggcaag gtcatcgagc agctcctcga cgcgggggcc ggtacggccg acgaatcctc
     1141 gtggacgcgg gacgtccgcg acgaggaaca gcggctgcag ggcgaggccg aagccggcat
     1201 gaacgcggac gccgtgccga tccacccgca gcggttcgcc cgggagatcc gcgacttcct
     1261 cgaccgcgac gcgatcgtcg tgggtgacgg cggcgacatc gtcggcatct ccgcctcgat
     1321 catccaggcg cacgcgcccg ggcactggct cgacaccggc ccgttcggct gcctgggcgt
     1381 cggcccctcg ttcgccatcg ccgcgaagct ggcccggccc gacaagcagg tggcgatcgt
     1441 ctacggcgac ggctcgttcg ggctgaacgc gatggagtac gagagcgcga tccgccagga
     1501 catcccgttc gtcgggatca tcggcaacga cggtgcctgg ggccagatca aggttgcgca
     1561 ggaggcgatg tacgggcagg ggaacgcgcc ggccgcgggg ctgtccaccg ccacgccgta
     1621 ccacaagatg gtcgagggtc tcggtgggta cggcgaactg gtgaccgagc ccggcgagct
     1681 ccgtcccgcc ctgcagcggg ccttcgacgc gggcgtcccg gcgtgcctga acgtggtcat
     1741 cgacccgacg ctgatgaagc gggcgtcgta cctgggctag cggtgcgcgc cgacaacttc
     1801 cccgaccccg accggccgcg aaggcgcttc ggcctcttcc ggagccgcgc ggagaagaag
     1861 gggaagcagg ggaagggacg cggccgggcc gccaagcccg acgccggcac cgtgaagtcc
     1921 atcgaggagt tcatcgcaac gcgggtcggg gtggaggcgt acctggagcc cgagaccccg
     1981 acgaacccgc tgtcggtggt gttcgtcgcc gccgacggag agtggatccg caagcggatc
     2041 cccgacgcgg cctggctgtc gaaggtttcg aaggccgctc ggatgccggt gtacgaggtg
     2101 gctcgcgtcg gctacccgcg gcggatgcgg gagtaccgcc gccgcccgcc gggctcgccg
     2161 gacgctcccg gccccacgga cctgccggac ctgcccgagc cgtgacgagg gtcacccggg
     2221 gcaggcgctc gcctggcgaa ccgggcgcca cctgctgcgc gcgccgttgg ccggcggggt
     2281 cgtcgaggtc gcccgccgcg tcgcgggcgt ccaggcgcag gtgatgtcga atgcggagct
     2341 cgccatcggc atccgggcga ggggcgtgac gccggcgaag gtccgggcgg tgcagcggca
     2401 gcgctcgctg gtgaagacct gggcgttccg cggcacgatc cacctgctgc ctgccgacga
     2461 gctcccgatg tggatcgcgg cgctcggcac gtggaccggc tatcggcggg gcgcctggct
     2521 gaaatggttc cgcgtgacgg cggaggaatt ggacgcggtg ctcgagggga tcgggcgcgc
     2581 cctcgacgga cggaacttga cgcgagaaca gctggcgcgg gaggtggctc gcgtgacgaa
     2641 gcgccggcac ctcgaggaga ttgtgaaggg ctcgtggggg tcggctctca agccggccac
     2701 gcggcaggga ctgctgatct tcggcccggt ggaagggcgc aacgtgacgt tcgtccgtcc
     2761 cgaccagtgg gtgaaggggt ggcacacggc cgacccagag gcatcgatgg acgaggcgat
     2821 tcgccggtac ctcgacgcgt acggccccgc gacccacgag gacttcgccc gttggttcgg
     2881 tctggacctc gctccgtccc gcaagcgctt cctgcgtctg gcggaggagc tcgtcgaggt
     2941 cgaggtggac ggtgaccgtg catggatggg gtgtcgcgcc agtccggctg gatctccccg
     3001 gtcctcctgg tcgacggcct gatcgccggc gtgtggtccc acgaggccgg ggggaaagcg
     3061 cctcagcatg aggatccaac cgttcgagcc ggcgccgaag cgcgtccgca cagcggcgga
     3121 gcgtgaggcc aatcgatacg gcgctctcct cggggccgca ccgacggtga cgcgggcata
     3181 gccccgggcc gtgtgtgagg agcttcacat catcgctggc tggagtggcc cctgacggcc
     3241 gtttcgtgtc agtaccccct gctagggtgt cgaacagatg ttcgagggtc aggaggggga
     3301 ccgctgagca cgcttcgctc ggccctggac gagctccggg gcgaggatct gtcgttcgtc
     3361 gaggacgcgt ccgtggaggc cgacttcggg gagctgcagc gggcctccga ggccctggag
     3421 gtggagaagc tgcgccggct ggccgagatc cgcaggcgcc gcacctacga acgggacggg
     3481 ttcgtctcgg cctcggcctg gctggtggac cgccatcgga tggccggcgg ggccgccgcc
     3541 cgggccctgg gatggcgcgg ttcctggatc gctcgcccga cactgccgag gccctggggc
     3601 agggcgagat ctcgctgtca gcggccgagg cgctgcgggc ggccgccgag accagtccgg
     3661 cggagtacgg ccggagcgag cgggtcctgc tcgaggcggc ccggaccctc tcggtccggg
     3721 accttttacc ggatggtcgg ctactggcgc cagcaggccg acgggcgcca ccccgagcgg
     3781 cgcttcgagc gccgcggcct gcatgtctcc cccacggtgt tcgggatggt ccgggtggac
     3841 ggggacctcg accccgagaa cggccaggcc ctcatcaccg cgatccgcgc ggtggtggac
     3901 gccgagaccc gctccggcga cgaatcggat acccgtactc cctcccagcg ccgggccgac
     3961 gccctcggcg agatctgccg gcggcacctg gcctgcgccg atcgccccct ggtcggtgg
//
LOCUS       DASPWO010000366         4003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_134845, whole genome shotgun sequence.
ACCESSION   DASPWO010000366 DASPWO010000000
VERSION     DASPWO010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134845"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VEO00_08390"
     CDS             <1..321
                     /locus_tag="VEO00_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58054.1"
                     /translation="SWADEFGITQAQAEAALKQAFANGDLSASADIGAYLTAVGSLLA
                     LSAAICGTAAYKKRTLAVPPPPPQAGAYPAAPPGPAPTAPQWTPPPAGPPPGGPPPAA
                     PPAP"
     gene            448..1605
                     /locus_tag="VEO00_08395"
     CDS             448..1605
                     /locus_tag="VEO00_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HYU58055.1"
                     /translation="MDIEIGKGKVARRGYGFDDVAIVPSRRTRDPDDVDISWEIDAYR
                     LELPLLASAMDGVVSVESAVGIGKLGGLAVLNLEGLQTRYEDPDPVFEEIAAQPPEKA
                     TRRMQELYQEPVKEELIVRRIREIKDAGVVSAASVTPQRTQRYAPLVLEAGLDILVIQ
                     GTVVSAEHVSTRTEPLNLKEFIARFDIPVIVGGCASYHTALHLMRTGAAGILVGVGPG
                     AACTTRGVIGVGVPQATAIADAAGARMQHLHETGRYCHVIADGGMRTGGDIAKAIACG
                     ADALMIGSPLAKAYEAPGRGFHWGMATFHPTLPRGTRVATRQIGTLEEILVGPAHEND
                     GTLNLFGALRMSMATTGYANIKEFQKAEVIVAPAITSEGKDLQRAQHVGMG"
     gene            1608..3137
                     /gene="guaA"
                     /locus_tag="VEO00_08400"
     CDS             1608..3137
                     /gene="guaA"
                     /locus_tag="VEO00_08400"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HYU58056.1"
                     /translation="MRPVLVVDYGAQYAQLIARRVRECHVYSEIVPHDLPVDDLVRRR
                     PAGLILSGGPASVYEPGAPFVDARLFGIGVPVLGICYGQQLMARSLGGEVAHTGAREY
                     GRTELRVTTPGLLLRDLPDEQVVWMSHGDTVVRAPDGFVATASTDSSPVAAMEDPQRG
                     LFAVQFHPEVAHTPRGLDLMKRFLYEACGLLPDWTPVTVVERAVEEVRAQVGGSSHVL
                     CALSGGVDSAVAALLVHRAIGDRLTCVFVDTGLLRQGEPEQVVETFQGHFGIDLVHVK
                     SEDRFLGRLAGVTDPEEKRKRIGEEFIRVFEEVARGHAEARFLVQGTLYPDVIESGTR
                     DAARIKSHHNVGGLPDEMDLELVEPLRLLFKDEVRKVGEELGLPEEIVWRQPFPGPGL
                     AVRIVGEVTRERLAIVRGADTVVQEEVRRAGLGGRLWQAFAVLPAIRSVGVMGDGRTY
                     HYPVVVRAVESEDAMTADWARLPWEVLERISSRIVAEVPGVNRVVYDVTSKPPGTIEW
                     E"
     gene            3525..>4003
                     /locus_tag="VEO00_08405"
     CDS             3525..>4003
                     /locus_tag="VEO00_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HYU58057.1"
                     /translation="MEALEVESLRRLAEIRRRRTYERDGFVSASAWLVDRHRMAGGTA
                     ARAGSCSRRPGPSRSGTFYRMVGYWRQQADGRHPERRFERRGLHVSPTVFGMVRVDGD
                     LDPENGQALISAIRAVVDAETRSGDESDTRTPSQRRADALGEICRRHLACADRPLVGG
                     "
BASE COUNT          545 a         1361 c         1515 g          582 t
ORIGIN      
        1 agctgggccg acgagttcgg tatcacgcag gcccaggccg aggccgccct caaacaggcg
       61 ttcgccaacg gcgatctctc cgccagcgcg gacatcgggg cctacctcac ggcggtcggg
      121 agcctgctgg cgctctccgc ggcgatctgt ggcaccgccg cctacaagaa gcgcacgctg
      181 gctgtcccgc cgcccccgcc gcaggccggc gcctaccccg cggccccgcc cggcccggcg
      241 ccgaccgcgc cccagtggac cccgcccccg gctggtccgc cgccgggcgg cccaccaccg
      301 gccgcgccgc cggctccgta gcgctcgcaa cgtcccgggt tggggctggt cggtgaccgg
      361 ccccaaccct ggtatcgttc cgtcatggag cacctcgacg cctgcccttc ccgggcgggc
      421 gtctcgcgtt tgtagagagg acgggggatg gacatcgaga tcggcaaggg aaaggtggcc
      481 cgccggggct acgggttcga cgacgtggcg atcgtcccca gccgccggac ccgcgacccc
      541 gacgacgtcg acatctcctg ggagatcgac gcctaccgcc tcgagctgcc gctgctggcc
      601 tcggccatgg atggggtggt gagcgtcgag tcggccgtcg gcatcgggaa gctcggcggc
      661 ttggcggtgc tgaatctcga aggcctgcag acgcgctacg aggatccgga tccggtgttc
      721 gaggagatcg ccgcccagcc gccggagaag gccacccggc ggatgcagga gctgtaccag
      781 gagccggtca aggaggagct gatcgtccgg cggatccggg agatcaagga cgcgggggtg
      841 gtgtcggcgg cctcggtcac gccgcagcgc acgcagcgct acgcccccct ggtgctcgag
      901 gccgggctgg acatcctggt catccagggg acggtggtgt cggccgagca cgtctcgacg
      961 cgaacggagc ccctcaacct gaaggagttc atcgcccggt tcgacatccc ggtgatcgtc
     1021 ggcgggtgcg cctcctacca caccgcgctg cacctgatgc gcaccggcgc ggccggcatc
     1081 ctggtagggg tagggcccgg ggcggcgtgc accacgcgcg gggtgatcgg ggtgggggtg
     1141 ccgcaggcca cggcgatcgc cgacgcggcc ggcgcgcgga tgcagcacct tcacgagacc
     1201 ggccgctact gccacgtgat cgccgacggc gggatgcgca ccggcgggga catcgccaag
     1261 gcgatcgcct gcggggccga cgcgttgatg atcgggtcgc cgctggcgaa ggcgtacgag
     1321 gcgccggggc gtgggttcca ctgggggatg gcgacgttcc accccacgct gccgcggggg
     1381 acgcgcgtgg cgacccgcca gatcgggacg ctcgaggaga tcctggtcgg accggcgcac
     1441 gagaacgacg ggacgctcaa cctgttcggg gcgctgcgca tgtcgatggc cacgaccggc
     1501 tacgcgaaca tcaaggagtt ccagaaggcg gaggtgatcg tcgctcccgc gatcacgtcg
     1561 gaggggaagg acctgcaaag ggcccagcac gtggggatgg ggtagccgtg cggccggtcc
     1621 tggtcgtcga ctacggcgcg cagtacgcgc agctgatcgc gcggcgcgtc cgcgagtgcc
     1681 acgtgtactc cgagatcgtc ccgcacgacc ttccggtcga cgacctggtt cgccgccggc
     1741 cggccgggct gatcctgtcc ggcggcccgg cgtcggtgta cgagccgggc gcgccgttcg
     1801 tcgatgcccg cctgttcggc atcggcgtcc cggtgctcgg gatctgctac ggccagcagc
     1861 tgatggcacg ctcgctgggc ggcgaggtcg cgcacaccgg cgcgcgcgag tacgggcgca
     1921 ccgaactgcg cgtgacgacg cccgggctcc tgctgcgcga cctccccgac gagcaggtcg
     1981 tgtggatgtc ccacggggac acggtggtcc gcgccccgga tgggttcgtc gccacggcgt
     2041 cgaccgactc gtcgccggtg gcggccatgg aggacccgca gcgcggcctg ttcgcggtgc
     2101 agttccatcc cgaggtggcg cacacgccgc gcggcctcga cctgatgaag cggttcctgt
     2161 acgaggcctg cgggctgctt ccggactgga cgccggtcac ggtcgttgag cgcgcggtgg
     2221 aggaggtccg ggcgcaggtc ggaggctcgt cgcacgtcct ctgcgccctg tcgggcgggg
     2281 tggactcggc ggtggcggcg ctgctcgtgc accgcgcgat cggcgatcgg ctgacgtgcg
     2341 tgttcgtcga taccgggctg ctccgccagg gcgagcccga gcaggtcgtg gagacgttcc
     2401 agggccactt cgggatcgac ctggtgcacg tcaagtccga ggaccgcttc ctgggccgcc
     2461 tggccggcgt gaccgaccct gaggagaagc gcaagcggat cggcgaggaa ttcatccgcg
     2521 tgttcgagga ggtcgcgcgc ggacacgccg aggctcggtt cctcgtgcag gggacgctgt
     2581 acccggacgt gattgagtcc ggcacccggg acgccgcgcg gatcaagagc caccacaacg
     2641 tcggcgggct gcccgacgag atggacctcg aactggtcga gccgctgcgc ctgctgttca
     2701 aggacgaggt gcggaaggtg ggggaggagc tcggcctgcc ggaggagatc gtctggcgcc
     2761 agccgttccc cggcccgggc ctggcggtgc gcatcgtcgg cgaggtgacg cgcgagcggc
     2821 tggcgatcgt ccgcggcgcc gacacggtgg tgcaggagga ggtccgtcgc gcaggcctcg
     2881 gcggacggct gtggcaggcg ttcgcggtgc tgccggcgat ccgcagcgtg ggcgtgatgg
     2941 gcgacgggcg aacctaccac tacccggtgg tggtgcgtgc cgtggagagc gaggacgcca
     3001 tgaccgccga ctgggcccgc ctgccatggg aggtgctgga gcgtatctcc tcccgcatcg
     3061 tcgccgaggt cccaggcgtc aaccgcgtcg tctacgacgt caccagcaag ccccccggca
     3121 cgatcgagtg ggagtagccg ggccggcagg aagcctcgcg ccatgctgat gtcgatcggt
     3181 cgctgatggt cgggtggttc gtgtcatcgg aagtgtggtc cacgctcgcg ttcgtccgcc
     3241 ccctcacggt gatcgtgggc gtcgcttgga tccttatcgg gatcttcgac ttcgcgctgc
     3301 ggtgagcatc gtgtgagcgg tttcacatcg cacgctggct ggagtggccc ctgaaggacg
     3361 ctccgtgtcg gtaccccctg ctagggtgtc gaacagatgt tcgagggtgc ggaaggggac
     3421 cgctgagcac gcttcgctcg gccctggacg aactcagggg tgaggatctc gggacggtcg
     3481 acgacggatc gctggaggcc gacttcgccg agacacgccg agcgatggaa gctctggaag
     3541 tcgagagcct gcgccggctg gccgagatcc gcaggcgccg gacctacgag cgggacgggt
     3601 tcgtctcggc ctcggcctgg ctggtggatc gccatcgcat ggccggcggg accgccgccc
     3661 gggcggggtc ctgctcgagg cggcccggac cctctcggtc cgggaccttt taccggatgg
     3721 tcggctactg gcgccagcag gccgacgggc gccaccccga gcggcgcttc gagcgccgcg
     3781 gcctgcacgt ctcccccacg gtgttcggga tggtccgggt ggacggggac ctcgaccccg
     3841 agaacggcca ggccctgatc agcgcgatcc gcgcggtggt ggacgccgag acccgctccg
     3901 gcgacgaatc ggatacccgt actccctccc agcgccgggc cgacgccctc ggcgagatct
     3961 gccggcggca cctggcctgc gccgatcgcc ccctggtcgg tgg
//
LOCUS       DASPWO010000367         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_394358, whole genome shotgun sequence.
ACCESSION   DASPWO010000367 DASPWO010000000
VERSION     DASPWO010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394358"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..2554
                     /locus_tag="VEO00_08410"
     CDS             455..2554
                     /locus_tag="VEO00_08410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HYU58058.1"
                     /translation="MAIRTAPRRHGARREPPRESPPKPEKRKGFVRRFWWLLLPALIL
                     IGMAGTLVYAYIKIELPEAPPPALTTYLYDRDGKLITTLHAEIDRTEISLKRMPEHLR
                     QAVIAMEDRGFYDHPGVSVFGIIRAAWADFRNRSIEQGGSTITQQYVKTVYTGGERTV
                     LRKIKEAILALKLENKYSKDEILEKYLNTIYFGRGAYGVEAAAETYFGKHAKDLNVVQ
                     SATLAGVIAAPEKFNPIVDPGAAKIRRDEALDAMVEEGYLARSDAERYKDRGVNVIEP
                     GSAAQTGPAAYFADYTRRFLQDEFGYETTFTGGLRVKTTLDLELQRAAEGAVSNHLSN
                     PGDPNAALVSIDPRTGDIVAMVGGKNFKKAKFNLATQAKRQAGSSFKTFTLAAAMEDH
                     ISPFSTWQGPGSITIDDPRCEGPDGEKWSPSNYSDSSSGTLSLVGATAGSVNTVFAQL
                     VTQVDPSDVAEIAHRLGIRSRLRPFCSITLGTQEVTPFEMTSAYATIAAQGVYREPRP
                     VTEVSAAGYKKVVFKPTRERAIRQNDADLVIYMEKNVVTGGTGTNARLASGMPVFGKT
                     GTSEENADAWFCGATKQLATCVWVGYKAGRVPMHDIGGYSSVTGGSIPALIWHDFMTV
                     AMQGMPIVDWPEPSFDGYNVGAFGHPYEPTTIPPQPTPKPKPKPKPKHSPKPPPSPSV
                     EPSPTVTVTPTESPSTD"
     gene            complement(2600..3247)
                     /locus_tag="VEO00_08415"
     CDS             complement(2600..3247)
                     /locus_tag="VEO00_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU58059.1"
                     /translation="MESGPVLIRVTIADDHSMVRQGLQRYLDLADDIEVVGQASNGRD
                     AVRMVEELRPDVALLDIQMGEPDGLEAARRIADLDPRIGIIMLTALEDRRHVVEAVTV
                     GARGYVLKARDAEHLIQTVRLVAAGNLVIDPDLVGVLSDEIRAGRAPGREGLTDREVQ
                     ILQLLAFGRTNREIGDTLFISPDTVKTHLEHIYEKLGTSDRTAAVAQAFRKRLIE"
     gene            complement(3253..>3988)
                     /locus_tag="VEO00_08420"
     CDS             complement(3253..>3988)
                     /locus_tag="VEO00_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58060.1"
                     /translation="SESMFFLLALTALWGARRRHWSVAGLAAAGAALTRNVGVLLAVP
                     LAVEAFLQFRTERLAPSAPVPDPMEPERGPGWRLFLRLLTSTLPAVALLGYLWFWELL
                     SGDPHAPIHDQTQWERVASFPIVTLGKASREAFRWIGIYPGGYHLLDWLIVMPVLVAC
                     GYALVRFRPTFGLYAWVTLLAPLSFIFEGRPFMSLPRIVLPIFPLYWALARFGERRGV
                     HEAIVAASAAGLGLMTVLFVTWYYVF"
BASE COUNT          672 a         1415 c         1382 g          519 t
ORIGIN      
        1 cgccgcccga accggccgaa cgggcggagg cgcggcccga gccggccgtg gccgcgaggg
       61 cgcgcgaggc gagggcttgg ggcgtagctc gcccgtgacg cccaccgacg ggccacgatc
      121 ggcgagcgag cccccgagga ggcgaggaga aacccccgac cgggtccgcc gggtgctggc
      181 cggcgccggc cgggctgccg ccgctgccgg gcgggggctt catcgcttgc gcgccgccgg
      241 ttcggcctgg ggcgccgtga tcctgctcga gatcgcggcg atcgtgctgg ccgtgcgcct
      301 ggtgatcgtc ggtgcgagca cggggttcct ctgacacccg ggggttcggg gggtagctcg
      361 ccccgggtgg gaccagcccg atcacgtccg ccgcgtgaat ccctcaaggg agacgggggc
      421 cttccgcgga tcgatcggta caatgctgcg gatcgtggcg atccgaactg ctccgcgcag
      481 gcacggcgcc cggcgcgagc ccccccgcga atctcctccc aagccggaga agcggaaggg
      541 cttcgtccgg cgcttctggt ggctcctgct gccggccctc atcctgatcg ggatggccgg
      601 cacgctcgtc tacgcctaca tcaagatcga gctgcccgag gccccgcccc cggcgctcac
      661 cacctacctc tacgatcgcg acggcaagct gatcaccacg ctccacgccg agatcgaccg
      721 caccgagatc tcgctgaagc ggatgcccga gcacctgcgg caggcggtga tcgccatgga
      781 ggaccgcggc ttctacgacc accccggcgt cagcgtcttc gggatcatcc gtgcggcgtg
      841 ggccgacttc cgcaaccgct cgatcgagca gggcggctcg acgatcacgc agcagtacgt
      901 gaagaccgtc tacaccggcg gcgagcgaac ggtcctgcgc aagatcaagg aggccatcct
      961 ggcgctgaag ctcgagaaca agtactcgaa ggacgagatc ctcgagaagt acctgaacac
     1021 gatctacttc ggccgcggcg cgtacggggt ggaggccgcc gccgagacct acttcggcaa
     1081 gcacgccaag gacctgaacg tcgtccagtc ggcgacgctc gccggcgtga tcgcggcgcc
     1141 cgagaagttc aatccgatcg tcgacccggg tgcggcgaag atccgccgcg acgaggcgct
     1201 cgacgcgatg gtcgaagaag gctacctggc gcgttccgac gccgagcgct acaaggaccg
     1261 cggcgtgaac gtgatcgagc ccggcagcgc cgcccagacc gggcctgcgg cgtacttcgc
     1321 cgactacacg cggcggttcc tgcaggacga gttcggctac gagaccacgt tcaccggcgg
     1381 cctgcgggtg aagaccacgc tggacctgga actgcagagg gccgccgagg gcgccgtcag
     1441 caaccacctg tccaacccgg gcgacccgaa cgcggcgctc gtctcgatcg acccccggac
     1501 cggcgacatc gtggcgatgg tcggcggcaa gaacttcaag aaggcgaagt tcaacctggc
     1561 aacgcaggcg aagcgccagg ccggaagctc gttcaagacg ttcacgctcg ccgcggcgat
     1621 ggaggaccac atctcgccgt tctccacttg gcagggcccg ggatcgatca cgatcgacga
     1681 cccgcgctgc gagggtcccg acggcgagaa gtggtcgccg agcaactact ccgactcgag
     1741 ctcggggacg ctcagcctgg tcggcgccac cgccggatcg gtgaacacgg tcttcgcgca
     1801 gctcgtcact caggtcgacc cctcggacgt cgccgagatc gcccaccgcc tcgggatccg
     1861 gtcgcgcctg cggccgttct gctcgatcac tctcggcacg caggaagtga cgccgttcga
     1921 gatgacgagc gcgtacgcga cgatcgcggc gcagggcgtc taccgggagc cgcgcccggt
     1981 caccgaggtc tcggcggccg gctacaagaa ggtcgtgttc aagccgaccc gcgagcgggc
     2041 gatccgccag aacgacgccg acctggtgat ctacatggag aagaacgtgg tcaccggcgg
     2101 cacggggacg aacgcccggc tcgcctccgg catgccggtc ttcggcaaga ccggcacctc
     2161 cgaggagaac gccgacgcgt ggttctgcgg cgcgacgaag cagctggcga cgtgcgtctg
     2221 ggtcggctac aaggccggac gggtgccgat gcacgacatc ggcgggtact cgagcgtgac
     2281 cggcggctcg atccccgcgc tgatctggca cgacttcatg accgtcgcca tgcagggcat
     2341 gccgatcgtg gactggcccg agccgagctt cgacgggtac aacgtggggg cgttcggcca
     2401 tccctacgag ccgaccacca tcccgccgca gcccacgccg aagcccaagc cgaagccgaa
     2461 gccgaagcac tcaccgaagc cgccgccgag cccgagcgtg gagccgagtc ccaccgtgac
     2521 ggtcacgccg accgagtcgc cgtccacgga ctgacccgcg cggaaccggg tccgcccccc
     2581 gattagtaca gctcctgtac tattcgatca agcgcttgcg gaacgcctgg gcgaccgcgg
     2641 cggtgcggtc gctggtaccg agcttctcgt agatgtgctc cagatgcgtc ttcaccgtgt
     2701 cgggtgagat gaacagcgtg tcgccgatct cgcggttcgt ccggccgaag gcgagcagct
     2761 gcaggatctg cacctcgcgg tcggtcaggc cctcgcggcc gggcgcccgt ccggcgcgga
     2821 tctcgtcgga cagcaccccg accaggtccg ggtcgatcac caggttgccc gcggcgacca
     2881 ggcgcaccgt ctggatgagg tgctcggcgt cgcgcgcctt cagcacgtag ccgcgcgccc
     2941 cgacggtgac ggcctccacc acgtgacggc ggtcctcgag cgcggtgagc atgatgatcc
     3001 cgatgcgcgg gtcgaggtcg gcgatccgcc gcgccgcctc caggccgtcc ggttcgccca
     3061 tctggatgtc gagcagcgcc acgtcggggc gcagctcctc gaccatccgc acggcgtcgc
     3121 gcccgttcga ggcctgtccg accacctcga tgtcgtcggc caggtccagg tagcgctgca
     3181 gcccctggcg gaccatcgag tggtcgtcgg cgatcgtgac tcggatcaac actggcccgc
     3241 tctccatggt gactagaaga cgtagtacca ggtcacgaac aggaccgtca tcaggcccag
     3301 cccggcggcg ctcgccgcga cgatcgcctc gtgcacgccg cggcgctcgc cgaagcgcgc
     3361 cagtgcccag tagagcggga agatcggcag cacgatgcgc gggagcgaca tgaacggccg
     3421 tccctcgaag atgaacgaca gcggtgcgag gagcgtcacc cacgcgtaca ggccgaaggt
     3481 gggccggaag cggaccaggg catagccgca ggcgaccagc accggcatca cgatcagcca
     3541 gtcgagcagg tggtagccgc cggggtagat cccgatccac cggaacgcct cacggctggc
     3601 cttccccagc gtcacgatcg ggaacgaggc gacccgttcc cactgcgtct ggtcgtggat
     3661 cggtgcgtgc ggatcgcccg acagcagttc ccagaaccac aggtagccga gcagcgccac
     3721 ggccggaagg gtggacgtca agaggcgcag gaacaggcgc caccccggcc cgcgctcggg
     3781 ctccatcgga tcggggaccg gcgccgacgg ggccaggcgc tccgtccgga attgcaggaa
     3841 ggcctcgacg gcgaggggca ccgccagcag cacgccgacg ttgcgggtca gcgccgcgcc
     3901 cgccgcggcc aggccggcca ccgaccagtg ccgacgccgc gccccccaca gggcggtcag
     3961 cgccagcagg aagaacatcg actcggag
//
LOCUS       DASPWO010000368         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1238468, whole genome shotgun sequence.
ACCESSION   DASPWO010000368 DASPWO010000000
VERSION     DASPWO010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238468"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VEO00_08425"
     CDS             <1..509
                     /locus_tag="VEO00_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HYU58061.1"
                     /translation="LFFLKAGAFIFGSGLAIVPFLREGVVVQHHWLTARQFLDAVAIG
                     LITPGPVVITAAFIGYLVGGFAGAGIATVAIFLPIYLGVVIPGRWFIRHKDSRRVQGF
                     VKGATAAAAGAIAGATIVLARGAIVDWPTAGIAVVALGFVWRFKNKEPVLVALAAAAG
                     LLLHPLAS"
     gene            complement(840..1091)
                     /locus_tag="VEO00_08430"
     CDS             complement(840..1091)
                     /locus_tag="VEO00_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58062.1"
                     /translation="MLLYDIVKPLVARARPPARFDVGYQFSGFAFPSGHATQSLAVWG
                     MLALLDAGMMRGRRYVPFAAATLSSASSVAPASTWARTG"
     gene            1524..2075
                     /locus_tag="VEO00_08435"
     CDS             1524..2075
                     /locus_tag="VEO00_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58063.1"
                     /translation="MTPRSILPPLLACVAFATAACGGGGTQGNPATTTPPPATTGEAV
                     DLTQAIRFHEGQAQVQVTGDVTESFTAALDTSSAGYTPAGGGDPAEIVIDWQAENGFL
                     FRIDFDVTDGTTIDRTWAAAGVPGTSISDENYFPDFFSTKCEGEIARFNASGLEGSVQ
                     CADLTNDAETKTVDLQTAFTATV"
BASE COUNT          385 a          768 c          754 g          311 t
ORIGIN      
        1 ggctgttctt cctcaaggcg ggggcgttca tcttcggaag cggcctcgcc atcgtgccgt
       61 tcctcaggga aggtgtcgtg gtccagcatc actggctcac cgcccgccag ttcctcgacg
      121 ccgtcgccat cgggctcatc accccgggcc ccgtagtgat caccgcggcg ttcatcgggt
      181 acttggtcgg cggattcgcc ggggcaggga tcgccactgt ggcgatcttc ctgccgatct
      241 acttgggcgt ggtcatcccc gggcggtggt tcatccggca caaggacagt cggagggtcc
      301 aggggttcgt caaaggggcc acggctgcgg cggccggcgc catcgccgga gcgaccatcg
      361 tgctcgccag gggagccatc gtggactggc cgacggcagg catcgcggtg gtggccctgg
      421 ggttcgtgtg gaggttcaag aacaaggagc cggtcttagt cgccctcgcc gcagcagcgg
      481 gccttctcct gcatccgttg gcaagctaaa cgcacgaaat ccggttgctg ctcagggggc
      541 acgcggtcaa gcaggaagca gctggtcccg acgggcatta gatcaggcgg gagatcgttg
      601 gtcgcagggc ccggaaatgg aaaccgccgg aacccgaagg tcccagcggt ctccgcgcgg
      661 aactgtacgc cctccccgcg gaagcggtca aggcctcttc gtcctcgacg atcaggccgc
      721 tcgctccacg atctcggcta tgcccacgtc gcgatcgggg gacctggctc gggcgagcat
      781 caccgccacg agtacgctga gccacagccc gctcagcgca tagccaccga ggacgtcgct
      841 cagccagtgc gcgcccaggt agatgcggga gccaccgacg aggccgacga cagggtggcg
      901 gccgcgaacg gcacgtatcg acgcccacgc atcatcccgg cgtcgaggag ggcgagcatc
      961 ccccagacgg ccaggctctg ggtggcgtga cccgagggga aggcgaaccc cgagaattgg
     1021 tacccgacgt cgaagcgagc gggcggccgg gcccgggcca caagcggctt gacgatgtcg
     1081 tacagcagca cccccgagca gcgacgctcc gagcttgccg agaggcttcc agtcccgccg
     1141 ccgaacgacg aagtagctgc caactaggag gacgatcggg atcagcacca cggtcgatcc
     1201 cagccaggtc acggacttca tcaggccggt ggcccacgcc gcccggtgcg ccgccacgaa
     1261 atgctcgaag cggggatcga tgcgagcgac ctcgtcgtgc gcgaccacgt cctgggtgag
     1321 ccccccgaac gcccacacgc agagagccgc gacggtgacg gcgaaggtaa gcaggaaccc
     1381 cttcgggcag cccggattca agcctactcc tgcaagatcc acccggggat gaagggcacg
     1441 atcaacgtca cctgatctgc gctggccgcc cggctgctaa gctggatcgg cgcctgccag
     1501 atacgcgcaa tcggagcaat cccatgaccc cgcgcagcat cctgccgccc ttgctcgcct
     1561 gtgtcgcgtt cgccacggcc gcctgtggcg gtggcggcac gcagggcaac ccggcgacca
     1621 cgacaccgcc gccggcgacg accggcgagg ccgtggacct cactcaggcc atccggttcc
     1681 atgaaggcca ggcgcaggtg caggtcaccg gcgacgtcac cgagtccttc accgcggcgc
     1741 tcgacacgtc gtccgcgggc tataccccgg ccggcggcgg cgacccggcc gagatcgtga
     1801 tcgactggca ggccgagaac ggctttttgt tccggatcga cttcgacgtc accgacggaa
     1861 ccacgatcga caggacgtgg gccgccgcag gcgtccccgg cacatcgatc agcgacgaga
     1921 actacttccc ggacttcttc agcacgaagt gcgagggtga gatcgcgcgg ttcaacgcct
     1981 ccgggctcga ggggtcggtc cagtgcgctg acctgacgaa cgacgccgag acgaagaccg
     2041 tcgacctgca gaccgcgttc accgcgaccg tctgaggccc gcggcggcac tcctggaacg
     2101 cggggcgcgg cggcggatcc gcttgcggac gatcggcgtc ccggcgctcc gccgctcgac
     2161 ctcccggaag cccgcgcgca gggacatcgg caacaggccg gtgtaggagc acgtcgga
//
LOCUS       DASPWO010000369         3410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_475342, whole genome shotgun sequence.
ACCESSION   DASPWO010000369 DASPWO010000000
VERSION     DASPWO010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475342"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            843..1145
                     /locus_tag="VEO00_08440"
     CDS             843..1145
                     /locus_tag="VEO00_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58064.1"
                     /translation="MPLDRDLIRSVRALDEYDLRRLMIFVRGLLLARDAEDPESRPGA
                     GARRGKVTFRQERVRCGRRGCTRCPHGPYWYAYWREGGRLRSRYIGKHLPAGAATS"
     gene            1279..1467
                     /locus_tag="VEO00_08445"
     CDS             1279..1467
                     /locus_tag="VEO00_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58065.1"
                     /translation="MTTLSAPGRCENCGSPDLLTVELSVGDQPVSFTACHECETKWWV
                     RAGARVPLRSVLEVAAQC"
     gene            1506..2840
                     /locus_tag="VEO00_08450"
     CDS             1506..2840
                     /locus_tag="VEO00_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003956784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HYU58066.1"
                     /translation="MRDLSGIFKAYDVRGVVPDELDEDAARSIGAAFAAFAAAPRIAV
                     GRDCRHSSPDLAAAFVDGATGRGVDVADIGLASTDMVYFASGELKVPGAMFTASHNPP
                     RYNGIKFCREGAAPVGEDTGLAEIRGLAEKGLDPLPEPGRVEPAGLLDRYVDRLLSFV
                     DVQALRPLTVAADAANGMAALTVPAVFERLPFELVPLYMELDGTFPNHPADPIDPRNQ
                     EDLRREVLRSGADVGLAFDGDADRVFLVDERAQGVSGSLVTALVAEAILEDEPGATIL
                     HNLICSWVVPEVVRERGGTAVRTRVGHSFMKQIMAETGAAFGGEHSGHFYFRDLYRAD
                     SGLLAALVVLERLSRATVPLSELLEPYRRYHASGEINSEVDDQAGAIERVARAFADGR
                     ADRLDGLTVEYEDWWFNVRPSNTEPLLRLNVEARTPELLEEKTNGVLAAIRS"
     gene            2853..3023
                     /locus_tag="VEO00_08455"
     CDS             2853..3023
                     /locus_tag="VEO00_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HYU58067.1"
                     /translation="MAVDKELLEILACPNCRGDVEYREAEQVIVCLKCGYRYPVRDDI
                     PVMLIEEAEKPA"
     gene            3020..>3410
                     /locus_tag="VEO00_08460"
     CDS             3020..>3410
                     /locus_tag="VEO00_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HYU58068.1"
                     /translation="MTAPSDPPAPGLAGDGGMLAVVGDLPRQVREGYAAGLAAPGLPS
                     VEGVRSIALLGMGGSAFPADLVQAQLEDRLGIPLVVSRGYRLPEFVHTDSLVLALSYS
                     GNTEETVTQYRDAVARGSRVVALCSGGE"
BASE COUNT          459 a         1195 c         1246 g          510 t
ORIGIN      
        1 tgaacgccgc cgacggcgcc ggcggggacg tggtggaggg ctcctcgggc gccgacacct
       61 gctcgaccga cccgggagac gtgatcatcg actgctgagc ccgcggagct ccggccatgt
      121 gggcgggagc cttccggccc ccgccctcgt tttgtcactg tttctggccc ggagggcgcc
      181 tccggcttgc gcggacgcga cggagaccgt agaatggtta ggtactccgg agagatcgga
      241 agccttcggg cggagggtca cttcggagta cgctcattcg cggacggggc ggacaccgcc
      301 tcttcctgcg cttttgggcc ggtcctcgcg aaacgagcgg cgatcgcggc ccttcaaccg
      361 atcttaggta acgagttcgc tacccaagtc ccagcccgtg cccccggatg attgaggcgc
      421 ctccgtccgt cctctatgct cgccgccatg cggtcgtgcg ccaagacgca gtgcaaccag
      481 ccggctgccg tcaccgcggc catgcggtac ggggagcgcg aggtggccct gttcgacctg
      541 ctggccgatc acgacccgaa ctacctgacc tgtgccgcgg tcacgccgat cgtctggtcc
      601 cgccgatcgg gtggtccgtg taggattccc gcaggggcgg gaacggcggg gcgctggcca
      661 cgtcggtcgt gggccgggcc ggcggtgagg ccgagctgcg cctggcgtcc ttcgagccgg
      721 gcgagggccg gccggaagct gatgagaccg gtccggcgct gcccttaacg ggcgatcttg
      781 ggtaagctgt tacccaaggc agatctttcc gagtccagcg aacctcacgg cgggagcttc
      841 ccgtgccgct cgatcgcgac ctgatccgga gcgtccgcgc gctcgacgag tacgacctgc
      901 ggcgcctgat gatcttcgtc cgcggcctgc tgctggcgcg agacgccgag gatccggaga
      961 gccggccggg agccggggca cggcgcggca aggtgacgtt tcgccaggag cgggtgcgct
     1021 gcggccggcg ggggtgcacc cggtgtccac acggcccgta ctggtacgcg tactggcggg
     1081 agggcggccg actgcgctcc cgctacatcg gcaagcacct tccggccggc gccgcgacga
     1141 gctgaacgtc ccccgcggcc cggccgatcg gcacggggac gatcggcgga caccctctag
     1201 tccttccgga ccacctgcag aaaccctccg gccgggctat gcaggaaccg caccccccaa
     1261 gccgacaatc ggggtgagat gaccacgctg tcggcccccg gacgttgcga gaactgcggc
     1321 tctcccgacc tgctcacggt cgaactgagc gtcggcgacc agccggtgtc gttcaccgct
     1381 tgccacgagt gcgagacgaa gtggtgggtg cgggccggcg ctcgcgtccc gctccgctcg
     1441 gtcctcgagg tcgccgccca gtgctgaccc cgaccgcgct ccgcccgcac ccgtagactc
     1501 ggtgcgtgag ggacctgtcc ggcatcttca aggcgtacga cgtgcgcggc gtcgtcccgg
     1561 acgagctgga cgaggacgcc gctcgcagca tcggggccgc cttcgccgcc ttcgcggcgg
     1621 cgccgaggat cgccgtgggc cgcgactgcc gccactcgtc ccccgatctc gcggcggcct
     1681 tcgtcgacgg ggccaccggc cgcggcgtgg acgtcgcaga catcgggctc gcctcgaccg
     1741 acatggtcta cttcgcctcc ggcgagctga aagtgcccgg cgcgatgttc acggccagcc
     1801 acaacccgcc gcgctacaac gggatcaagt tctgccggga gggggccgct ccggtgggcg
     1861 aggacaccgg gctggccgag atccgcgggc tggccgagaa gggcctcgat ccgctgccgg
     1921 agccggggcg agtcgagccg gccggcctgc tcgatcgcta cgtcgaccgc ctgctgtcct
     1981 tcgtggacgt ccaggccctg cgcccgctga cggtggcggc cgacgccgcg aacgggatgg
     2041 cggcgttgac ggttccggcc gtcttcgagc gcctgccgtt cgagcttgtg ccgctgtaca
     2101 tggagctcga cgggaccttc cccaatcacc cggccgaccc gatcgacccc cggaaccagg
     2161 aggacctgcg gcgcgaggtc cttcgatcgg gcgccgacgt cgggctggcg ttcgacgggg
     2221 acgccgaccg cgtgttcctg gtcgacgagc gcgcgcaggg ggtgagcggc tcgctggtca
     2281 ccgctctggt ggcagaggcc atcctggagg acgagccggg cgccaccatc ctgcacaacc
     2341 tgatctgctc gtgggtcgtc cccgaggtgg tccgcgagcg cggcgggacg gcggtgcgca
     2401 cccgcgtcgg ccactcgttc atgaagcaga tcatggccga gaccggggcg gcgttcggtg
     2461 gcgagcattc cgggcacttc tacttccgcg acctgtaccg cgccgactcc ggcctgctcg
     2521 ccgcgctggt cgtcctcgag cggctctccc gtgccacggt ccccctgtcc gagctgctcg
     2581 agccctaccg ccgctaccac gccagcggtg agatcaactc ggaggtcgac gaccaggccg
     2641 gcgcgatcga gcgggtggcg cgcgccttcg ccgacgggag ggcggaccgg ctggacggcc
     2701 tgacggtcga gtacgaggat tggtggttca acgtgcggcc ctcgaacacc gagccgctgc
     2761 tccggctgaa cgtggaggca cggacgccgg agctcctgga ggagaagacg aacggtgtgc
     2821 tcgccgcgat ccgatcgtag gagggacggg gaatggcggt cgacaaggag ctgctggaga
     2881 tcctggcctg cccgaactgc cggggggacg tcgagtatcg ggaggccgag caggtgatcg
     2941 tgtgcctgaa gtgcgggtac cgctacccgg tccgcgacga catcccggtg atgctgatcg
     3001 aggaggccga gaagcccgcg tgaccgcccc gtccgatccg ccggcccccg gcctcgccgg
     3061 agacggcggg atgctcgccg tcgtcggcga cctgccccgc caggtccggg agggctacgc
     3121 cgcaggcctg gcggctcccg gcctgccgtc cgtggagggc gtgcgatcga tcgcgctcct
     3181 cgggatggga ggctccgcgt tcccggcgga cctggtccag gcgcagctcg aggaccgcct
     3241 ggggatcccg ttggtcgtct cgcgcggcta ccggctcccc gagttcgtgc acaccgactc
     3301 gctggtgctg gcgctgtcgt actccgggaa caccgaggag accgtgacgc agtaccgcga
     3361 cgcggttgcg cgggggtccc gggtcgtggc gctgtgctcc ggcggggagc
//
LOCUS       DASPWO010000370         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_984396, whole genome shotgun sequence.
ACCESSION   DASPWO010000370 DASPWO010000000
VERSION     DASPWO010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984396"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VEO00_08465"
     CDS             complement(<1..195)
                     /locus_tag="VEO00_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58069.1"
                     /translation="MTTMHRTIQVDPTTNFDHAPVTVPAPREESEHDAEVELMHSIDQ
                     LVRTARHDPELKELILAWEDE"
     gene            409..687
                     /locus_tag="VEO00_08470"
     CDS             409..687
                     /locus_tag="VEO00_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58070.1"
                     /translation="MARWEQFEQCPHCGWNIATNEGERGCAWGDCPYLPEELNVFCDW
                     CRFDFATMEGNSPCDDPLTCEHGVVPRAHVENYHRWAAAREALTTPGA"
     gene            840..974
                     /locus_tag="VEO00_08475"
     CDS             840..974
                     /locus_tag="VEO00_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58071.1"
                     /translation="MLGAVALGYATESSLLDPLLVIGAVVLLPVWLLWTARSPGGEEA
                     "
     gene            complement(1012..1764)
                     /locus_tag="VEO00_08480"
     CDS             complement(1012..1764)
                     /locus_tag="VEO00_08480"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYU58072.1"
                     /translation="MKQLRVAGVMTRDVVTAREDTGFKDLVELMTGRAISAVPIVDDE
                     GRIAGIVSEADLLAVEAEEREPPRRRRFIEWFIDRRQLEAIERRAPSLSAADVMTRDV
                     ITARPDTPVEAAIKTLLRARVKRLPVVDDENRVVGIVSRRDLLSPFLREDEEILAEIR
                     DEVVERTMWLDPSTLDIEVQDGVVTLRGTVDRRSTTEILADLVHRVDGVVGVEDDLGF
                     EHDDRKDRPPHQPRPDVVEDTAEPGGYASRSP"
     gene            1831..2367
                     /locus_tag="VEO00_08485"
     CDS             1831..2367
                     /locus_tag="VEO00_08485"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HYU58073.1"
                     /translation="MARDGNGVKVTPPHPRHPLAATVVFLLFGGPAIVVAVVPWLLSG
                     FRVRPPFLGWEPWRWIGVALIALGSGLVLDALARFVRQGRGTPSPVGQTEHLVVSGAY
                     RFVRNPMYVAVIAILVGEALLLGSAAVLAWAAAVTALFAVWVRVYEEPRLRRLFGAEY
                     EAFCRRVPRWFPRLGARG"
     gene            2475..>3260
                     /locus_tag="VEO00_08490"
     CDS             2475..>3260
                     /locus_tag="VEO00_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58074.1"
                     /translation="MSTAHGSVRLALGFTLAAAAVIALALPAAAASDTEVTVGSVDPF
                     SGNKQNEPTVAIDPNHENLVVAGANDNIDLEDCNAGADSTCPFTAGVGTTGFQYSTDG
                     GTSWSQPTFTGYSARDCVGVNEPPGVAPTDACVPDEDGDIGTLPNYFENGVVSDGDPA
                     AAWGPTPGGGGFSWADGSRLYFANLTSNFSSKHSESSFKGVEAIGVSRVDVTSADPGT
                     QAAQVSDQGAWMAPSLISPGGAGFADKEQIWADNAESSEFFGNV"
BASE COUNT          429 a         1130 c         1208 g          493 t
ORIGIN      
        1 ctcgtcctcc caggccagga tcagctcctt cagctccgga tcgtggcggg cggtccgcac
       61 cagctggtcg atcgagtgca tcagctcgac ctcggcgtcg tgctcggact cctcgcgtgg
      121 agcgggaacg gtgacgggcg cgtggtcgaa gtttgtggtt gggtcgacct ggatcgtccg
      181 gtgcatcgtc gtcacctccg atggcctcct gcccgtgacg ctacgggctg cccgggcctg
      241 ccgggatggg tcggccggcc cccactggga gggccgaacg gtcctgggtg acgggggtgg
      301 ggacggcgga tcccgagggg ccgagcggcc cggcgggagg ggtccgatcg gccctccccg
      361 cccgtcccgc ccgctcctac ggtgcggaca ggacagaagg aggagaccat ggcgcgatgg
      421 gagcagttcg agcagtgtcc gcactgcggg tggaacatcg cgaccaacga gggcgagcgc
      481 ggctgcgcgt ggggggattg cccctacctg ccggaggagc tgaacgtgtt ctgcgactgg
      541 tgccggttcg acttcgccac gatggaggga aattccccgt gcgacgaccc cctcacctgc
      601 gagcacggcg tggtcccccg cgcccacgtg gagaactacc atcgctgggc cgccgcacgc
      661 gaggcgctga cgacaccggg cgcctgagac gacggcgggg gctcgccgtg acacgcgcgg
      721 cccgtcaggt ggaccggagg tacgcgagca ccgcgagcac gcgccggtga tcgtcggcga
      781 gggctcgagc ccgagcttgg cgaagatcga gccgatgtgg atcgctgctt ctcggcgggg
      841 tgctcggggc ggtcgcgctc gggtacgcca ccgagtcgag tctcctcgac ccgctcctcg
      901 tcatcggagc ggtcgtgctc ctccccgtct ggctcctgtg gacggcgcga tcgccgggcg
      961 gtgaggaggc gtgaccggcg atcgaggatg gcatcgggga tcgccgcacg ctcaggggga
     1021 tcgcgaggcg tagccgcccg gttccgccgt gtcctcgacg acgtcgggcc ggggttgatg
     1081 cggcgggcgg tccttgcggt cgtcgtgctc gaagccgaga tcgtcctcca cgccgacgac
     1141 gccgtcgacg cggtgcacca ggtcggcgag gatctccgtc gtgctgcggc gatcgacggt
     1201 cccccggagc gtgaccaccc cgtcctggac ctcgatgtcg agcgtggatg gatcgagcca
     1261 catggtgcgt tcgacgacct cgtcccggat ctcggccagg atctcctcgt cctcgcgcag
     1321 gaacggagag aggaggtccc tccggctgac gatgccgacc acccggttct cgtcgtccac
     1381 cacggggagc cgcttgaccc gcgcccgcag cagcgtcttg atggccgctt cgacgggggt
     1441 gtcgggccgc gcggtgatca cgtcccgcgt catgacgtcg gcggcggaga gcgagggcgc
     1501 ccgccgttcg atcgcctcga gctggcggcg gtcgatgaac cattcgatga agcgccgccg
     1561 ccgcggcggc tcacgctcct cggcctcgac cgcgagcagg tcggcctccg acacgatgcc
     1621 ggcgatccgg ccctcgtcgt cgacgatcgg gaccgcgctg atcgcccggc cggtcatcag
     1681 ctcgacgagg tccttgaacc cggtgtcctc gcgagccgtc accacgtccc gcgtcatcac
     1741 gccggccacg cgcagctgct tcatccacgt cctccgttcc gcggcccgcg gtggaaccgc
     1801 gggatcggct cctggtagac ccgtgcatgt atggcgcgag acgggaacgg cgtcaaggtg
     1861 acgccgccgc acccgcggca tccgctggcg gcgaccgtgg tgttcctcct gttcggcggt
     1921 ccggcgatcg tcgtcgcggt cgtcccctgg ctgctctcgg ggttccgcgt gcgcccgccc
     1981 ttcctcgggt gggagccgtg gcggtggatc ggcgtcgcgc tgatcgcgct cgggtcgggg
     2041 ctggtcctcg acgccctcgc ccggttcgtg cggcagggcc ggggaacgcc gtccccggtc
     2101 ggccagacgg agcacctggt cgtcagcggc gcgtaccgat tcgtgcgcaa cccgatgtac
     2161 gtcgcggtga tcgccatcct ggtcggcgag gcgctgctgc tcggcagcgc ggcggtgctg
     2221 gcctgggcgg ccgccgtgac cgccctgttc gccgtgtggg ttcgcgtcta cgaggaaccg
     2281 cgcctgcgcc ggctcttcgg cgccgagtac gaggccttct gccggcgggt cccgcgctgg
     2341 ttcccgcgac tcggcgcgcg cgggtaggcg gccgggcacg atcgtcgcgt tgaccccagg
     2401 gttgccggaa tcgatccgct atgcgagggt cgtccggcgc cccgcccgct cgacgacgag
     2461 aggggggatg tgatgtgagc accgcgcacg gatcggtgcg cctggcgctc gggttcacgc
     2521 tcgccgccgc ggcggtgatc gccctggccc tgccggcggc agcggcgtcc gacacggaag
     2581 tgacggtcgg cagcgtcgat ccattcagcg gcaacaagca gaacgagccg acggtcgcga
     2641 tcgaccccaa ccacgagaac ctggtggtgg caggggcgaa cgacaacatc gacctcgagg
     2701 actgcaacgc cggcgccgac agcacctgtc cgttcaccgc gggcgtcggc accaccggct
     2761 tccagtactc caccgacggc ggcaccagct ggagccagcc gaccttcacc gggtacagcg
     2821 cgcgggactg cgtcggcgtc aacgagcccc ccggcgtggc gcccacggac gcgtgcgttc
     2881 cggacgagga cggcgacatc gggacgctgc cgaactactt cgagaatggg gtcgtctccg
     2941 acggcgaccc ggccgcggcc tggggcccga ctcccggcgg cggcgggttc tcgtgggcgg
     3001 acggttcccg gctgtacttc gcgaacctga cgagcaactt ctcgtcgaag cactcggaga
     3061 gctcgttcaa gggcgtggag gccatcggcg tgtcgagggt cgacgtcacg tcggccgacc
     3121 cggggacgca ggccgcccag gtctcggacc agggagcctg gatggcgcca tcgttgatca
     3181 gccccggcgg ggccgggttc gcggacaagg agcagatctg ggccgacaac gccgagagca
     3241 gcgagttctt cggcaacgtg
//
LOCUS       DASPWO010000371         8981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_311171, whole genome shotgun sequence.
ACCESSION   DASPWO010000371 DASPWO010000000
VERSION     DASPWO010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311171"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VEO00_08495"
     CDS             complement(<1..825)
                     /locus_tag="VEO00_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU58075.1"
                     /translation="MSATVATVSGRVSLTRARVMGILFVLIGVAILVSFAGVSGASDS
                     RFVLNEARGNAIRLPDLVVPTRGTAMFLAAVCAFLGAIQLTRGFGTKVTLVLAVVVVL
                     FVFAFLTWAARDRSLNLLGMLQSTVLRSVPITFGALSGVLCERSGVINIGIEGMLLTA
                     AFTGAIVGSAASSLLAGLVAGALIGGLLGALLAVLSIRYRVDQIVAGTVINILALGLT
                     SFLGSAILARFQDLNNPGRFQNVALPFLSRIPLLGPMFFKQNIFVYLLIALVVLIHV"
     gene            complement(822..2108)
                     /locus_tag="VEO00_08500"
     CDS             complement(822..2108)
                     /locus_tag="VEO00_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU58076.1"
                     /translation="MPEPPPDPAPQDPAAGRPVVEAEPDDLTPAERGAHEEGAAPTAG
                     AIVTGGAADWRQALLVPALAFVAALAVGAIVIAFTNTEVLREWASFFRDPVGALRASW
                     QIVRDAYVALFQGSFGNVTAISETLVATTPLIFAGLAVALGFRAGLFNIGAEGQITMG
                     ALLGSLVGFSYPGLPDPLHVVLIMAGAFIGGAAWGFIPGILKARTGAHEVITTIMLNF
                     VASSFALWALTTELYRRPGREDPISVPVSAAFPHLFGSGQRVTIGLLVALGAAAAVAW
                     LVNRTTVGFEFKAVGANPFAARAAGMSPARTIVFVMSLAGGLAGLAGADLLLGIQPSM
                     TPGFSSGLGFDAIALALLGRARPAGVVLAALLFGALRSGARAMQAAAGTPVDIVMVIQ
                     ALVIAFVAAPALVRAIFRIRARRVAGPEAFTKGWGG"
     gene            complement(2101..3624)
                     /locus_tag="VEO00_08505"
     CDS             complement(2101..3624)
                     /locus_tag="VEO00_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU58077.1"
                     /translation="MRLELKGVTKRFPGVLANDRVDLTVEEGEIHALLGENGAGKTTL
                     MNVLYGLYHADEGEILIDGTPVRFNGPGDAIAAGLGMVHQHFMLIPVFTVTENIMLGV
                     EATGLAGLLKRDEAARHVVEISRQYGLEVPPGALVEELPVGVQQRVEIVKALYREAEV
                     LILDEPTAVLTPQETEELFEVLRSLKAAGKSVVFITHKLKEVLAVADRISVLHRGRLV
                     GTTTPDAASEEDLATMMVGRPVVLEVEKAAAKPGAVVLEVRDLLVRDERRQVAVNGIS
                     FDVRAGEIVCVAGVQGNGQTELVEALTGLRHVESGTISIWGINRTNATPRDVLRVDVG
                     HIPEDRQRNGLILDFSIADNLVLNEYDRAPFARSIVRQSRAVDDAARDRIKRFDIRTT
                     SIDAPASTLSGGNQQKVIVAREFSRQIRLLIASQPTRGLDVGSIEYIHSRIVEARDQN
                     AAVFVISSELDEVFSLADRIVVMYRGSIVETLPAAEASRERVGLLMAGSAVEESARA"
     gene            complement(3691..4806)
                     /locus_tag="VEO00_08510"
     CDS             complement(3691..4806)
                     /locus_tag="VEO00_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HYU58078.1"
                     /translation="MRRRVRMLAALVGVLALLAAACAEETPSGGGSASPKIGEGTLAC
                     EVTDVGGVDDKSFNATAYKGLTDAETEFGIEQRVLESQTDADYAPNVQTFLQQGCDLI
                     VTVGFLLGDATQAAAEDNPDRNFAIVDNNFFDFSTDPPTDVSFDNVKELTFQTDEAAF
                     LAGYLAAGMTQSGKVATYGGTNLPTVSIFMNGFVAGVRAYNQDNGTEVEALGWDPEAQ
                     DGTFISGDPNIGFDDQDAGRRLAEDFIAEEADIIMPVAGPSGLGGAAAAQDAGGVNLI
                     WVDVDGCVSAEEFCSLFLTSVEKHMDVAVKDQVKAVIDGDFTGGLYVGTLENDGVGIA
                     PYHEFDSEIPQELKDKIEELRAGIIAGDVSIDPADYA"
     gene            4934..5812
                     /gene="nadC"
                     /locus_tag="VEO00_08515"
     CDS             4934..5812
                     /gene="nadC"
                     /locus_tag="VEO00_08515"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HYU58079.1"
                     /translation="MASTDPADQASASRAALLKVVAQALEEDAPGGDLTSALTVPEGD
                     RVRADLRAKAPGVVAGTAAATAAFDLLAARDGGGPVRVEWNVREGDSVAPGTVLATVS
                     GSARTVLRAERVAINFLAHLSGVATLTRAFVEAAAPARVLCTRKTTPGLRALEREAVL
                     AGGGALHRASLSDAVLIKDNHLKVAGGVGPAVRAARAGGVPVEVEVETLAQLDEALEA
                     GADRILLDNPSPDLVRRAVARVGDPERLEVSGGITLDSVGELVAAGARIVSVGRLTHS
                     APALDLSLEVTDVDHV"
     gene            5796..6773
                     /gene="nadA"
                     /locus_tag="VEO00_08520"
     CDS             5796..6773
                     /gene="nadA"
                     /locus_tag="VEO00_08520"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017625705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HYU58080.1"
                     /translation="MSTTYEEWAAEVRRLAAERRAVILAHNYQVPWIQDVADHVGDSL
                     ALSREAAKTDAETIVFCGVHFMAETAKILSPEKTVLLPNLAAGCSLAATIDAEQLRAW
                     KGEHAGAVVVAYVNTSAEVKAESDICCTSSNAAEIVRTIPEDREILFLPDMFLGAHVE
                     RQTGRKLHVWMGECHVHAGISPQELRAKAETERDAEFMIHPECGCTTSALYLLDEGIL
                     PKDRTKVLSTGGMVAEAQRSGATRFLVATETGILHQLRKAAPGKVFEPIDRKAECQYM
                     KAITPENLLHALREGVYEITVPEEIRRRAERAVQRMIDLGPAPVPATTS"
     gene            6793..8250
                     /gene="nadB"
                     /locus_tag="VEO00_08525"
     CDS             6793..8250
                     /gene="nadB"
                     /locus_tag="VEO00_08525"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HYU58081.1"
                     /translation="MKTVVVGAGAAGLWAALHAVEQGPVVLVAPDPSAGSATALAQGG
                     IAAAVAEGDDPAAHAEDTLTAGAGLCDREAVHVLTAEAPDSIAELVRRGMAFDAGGRP
                     TLEGGHRARRVLHAGGDATGRFLLQALLAAADRERRVVWDRGRVAALLPEGGAVRGVR
                     RADGAEVEGDRTILATGGACGIYGRRTGPDHSTGHGLALAWDAGAALADLEFVQFHPT
                     ALDVPGHAARLLTEALRGEGGRLVDPRGRRFMADHDPRGDLAPRDVVSRAIAAVRAET
                     GRVFLDATGIDGVRERFPTAAATCDAVGLDLARDPVPVAPAAHYFIGGVLTDLWGRTT
                     VPGLSACGEVASTGVHGANRLASNSLLETLVFGRRAALAPEGTGPSAEPEDPYADLPG
                     TTGPSLEDVRSVADAALGVRRDAPTLEAALRYLATSGGGGAHALVARLVARAALRREE
                     SRGAHFRDDHPRTVEAWRRRQVVSPLGWCRLPVAS"
     gene            8333..>8981
                     /locus_tag="VEO00_08530"
     CDS             8333..>8981
                     /locus_tag="VEO00_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58082.1"
                     /translation="MLTREAGHRVRRIQSDAFPVLSVCLSLSPLPRDLASVRPRLKDL
                     LDLIRAAADPERLGHEAAMSVRADVERVLELEDRIHADVGHGVAVFACHGLGPFEYLS
                     LPRGLPDRASLDGRAQTGPLDAFLERSHRCVAVVVERDRSSIFEFHQGELEVHREVRD
                     GRIRKRNYGGRAGLDEYTVTRHAREEALRHHRRTVRAVGQLFSPRGSARRTRSSRA"
BASE COUNT         1257 a         3294 c         3214 g         1216 t
ORIGIN      
        1 gacgtggatc agcaccacca gcgcgatcag caggtagacg aagatgttct gcttgaagaa
       61 catcggcccg agcagcggga tgcgcgacag gaacgggagg gcaacgttct ggaaccgccc
      121 cgggttgttc aggtcctgga agcgcgccag gatcgccgat cccaggaagc tggtcaaccc
      181 gagcgccagg atgttgatca cggtcccggc cacgatctgg tcgacgcggt agcggatcga
      241 gagcaccgcc aggagcgcgc cgagcagccc cccgatgagc gccccggcga cgaggcccgc
      301 cagcaggctg ctcgccgcgc tgccgacgat cgcccccgtg aaggccgccg tgagcagcat
      361 gccctcgatc ccgatgttga tcacgcccga ccgctcgcaa agcacgccgg acaacgcgcc
      421 gaaggtgatc gggacgctgc gcaggaccgt gctctgcagc atcccgagca ggttgagtga
      481 gcgatcgcga gcggcccagg tcaggaacgc gaacacgaac agcacgacca ccacggcgag
      541 cacgagcgtc accttggtcc cgaacccccg cgtcagctgg atggccccca ggaaggcgca
      601 cacggcggcc aggaacatcg ccgtgccccg cgtcggcacg acgaggtcgg gcagccggat
      661 cgcgttccca cgcgcctcgt tcaggacgaa gcgcgagtcc gaggccccgc tgacgccggc
      721 gaaggacacc aggatcgcca ccccgatcag gacgaacagg atgcccatca cgcgcgcccg
      781 cgtgagagag acccgaccgg agaccgtggc cacggtggcg ctcacccccc ccaccccttc
      841 gtgaacgcct cggggccggc cactcgccgc gcccgtatcc ggaagatcgc ccggaccagg
      901 gccggcgcgg cgacgaacgc gatcacgagc gcctggatga ccatcacgat gtccaccggc
      961 gtcccggcgg ccgcctgcat cgcccgggcg cccgacctca gggccccgaa gagcagggcc
     1021 gccagcacga cgccggccgg ccgggcgcgc ccgaggagcg ccagggcgat cgcgtcgaac
     1081 ccgaggcccg aggagaagcc gggcgtcatg ctcggctgga tgccgagcaa caggtccgcg
     1141 ccggccagtc cggccagccc cccggcgagg gacatgacga acacgatcgt ccgcgcgggg
     1201 ctcatcccag ccgctcgggc cgcaaacggg ttcgccccga ccgccttgaa ctcgaagccg
     1261 accgtcgtcc ggttgaccaa ccacgcgacg gcggccgccg cgccgagcgc gacgagcagg
     1321 ccgatcgtca cccgctggcc cgacccgaac aggtgcggga acgccgcgct gaccggcacg
     1381 gagatcgggt cctcgcgccc gggacggcga tacagctcgg tcgtgagcgc ccagagcgcg
     1441 aacgaggacg cgacgaagtt cagcatgatc gtggtgatca cctcgtgcgc gccggtgcgc
     1501 gccttcagga tcccggggat gaacccccag gcggcgcccc cgatgaaggc tccggccatg
     1561 atcagcacca cgtggagcgg gtcggggagc cccggatagg agaacccgac caggctgccc
     1621 agcagggcgc ccatcgtgat ctgtccctcg gcgccgatgt tgaacagccc cgcccggaac
     1681 cccaaggcga cggccagccc ggcgaagatc aggggagtgg tcgcaaccag cgtctcggag
     1741 atggcagtga cgttcccgaa cgagccctgg aacagcgcga cgtaggcgtc gcgcacgatc
     1801 tgccaggagg cccggagcgc accgaccggg tcgcggaaga agctggccca ctcccgcagc
     1861 acctcggtgt tggtgaaggc gatcacgatc gcgccgacgg cgagcgccgc gacgaacgcc
     1921 agggcgggga ccagcagggc ctgccgccag tccgcggcgc ccccggtgac gatcgcaccg
     1981 gcggtggggg cggcgccctc ctcgtgcgcc ccgcgttcgg ccggagtgag gtcatccggc
     2041 tcggcttcca ccacgggcct gccggccgcc gggtcttgag gcgccgggtc gggcgggggt
     2101 tcaggcacgg gcggattcct cgacggccga tccggccatc agcaacccga cgcgctcgcg
     2161 gctcgcctcg gcggcgggaa gcgtctccac gatgctcccg cggtacatca ccacgatccg
     2221 gtcggccagg gagaagacct cgtcgagctc cgaggagatg acgaacaccg cggcgttctg
     2281 gtcccgcgcc tccacgatcc gggagtggat gtactcgatg gagccgacgt cgaggccccg
     2341 ggtgggctgc gaggcgatca gcaggcggat ctggcgggag aactcccggg cgacgatgac
     2401 cttttgctgg ttcccccccg agagcgtcga ggccggcgcg tcgatcgagg tggtgcggat
     2461 gtcgaagcgc ttgatccggt cgcgcgccgc gtcgtccacg gcccgggact ggcggacgat
     2521 gctccgcgcg aacggggccc ggtcgtactc gttcagcacc aggttgtcgg cgatcgagaa
     2581 gtccaggatc aggccgttgc gctggcggtc ctcggggatg tgcccgacgt cgacgcggag
     2641 cacgtcgcgg ggcgtggcgt tggtccggtt gatcccccag atggagatcg tcccgctctc
     2701 gacgtgccgc aggccggtca gcgcctcgac gagctccgtc tggccgttgc cctggacccc
     2761 cgcgacgcag acgatctcac cggcgcgaac gtcgaacgag atcccgttca ccgcgacctg
     2821 tcgccgctcg tcgcggacca gcaggtcgcg cacctcgagc acgaccgccc ccggcttcgc
     2881 cgcggccttc tcgacctcga gcaccaccgg ccgcccgacc atcattgtcg cgagatcctc
     2941 ctcgctcgcg gcatcgggcg tcgtggtgcc gaccagccgc ccgcggtgca ggaccgagat
     3001 tcggtccgcc accgccagga cctccttcag cttgtgcgtg atgaacacga cggacttgcc
     3061 ggccgccttc agcgagcgca ggacctcgaa cagctcctcg gtctcctgcg gcgtgagcac
     3121 ggcggtcggc tcgtcgagga tgaggacctc ggcctcccgg tacagggcct tgacgatctc
     3181 gacccgctgc tgcaccccga ccggcagctc ctcgaccagc gcgcccggcg ggacctcgag
     3241 cccgtactgc cggctgatct ccacgacgtg gcgtgcggcc tcgtcccgct tcagcagccc
     3301 ggccagcccg gtggcctcca cgccgagcat gatgttctcg gtcaccgtga agacggggat
     3361 cagcatgaag tgctggtgga ccatccccaa cccggcggcg atggcgtcgc ccggtccgtt
     3421 gaagcgaacc ggcgtcccgt cgatcaggat ctcgccctcg tcggcgtggt acaggccgta
     3481 gaggacgttc atcagggtcg tcttcccggc gccgttctcg ccgaggagcg cgtggatctc
     3541 gccctcctcg accgtcagat cgacgcggtc gttcgccagc acgcccggga agcgcttggt
     3601 gacgcccttc agctcgagcc gcatccggcc tcccgtcgag ggagggacac cgggcgtgcc
     3661 ggtgcccctc ccccgcgtac cctgcgactc ctacgcgtag tccgccgggt cgatggagac
     3721 gtcgccggcg atgatccccg ccctcagctc ctcgatcttg tccttcagct cctgggggat
     3781 ctcggagtcg aactcgtggt acggggcgat cccgacgccg tcgttctcga gcgtgcccac
     3841 gtacaggccg cccgtgaagt caccgtcgat gaccgccttg acctggtcct tgaccgcgac
     3901 gtccatgtgc ttctcgacgc tcgtcaggaa taggctgcag aactcctcgg ccgagacgca
     3961 gccgtcgacg tcgacccaga tcaggttcac gccgccggcg tcctgcgccg ccgccgcgcc
     4021 gccgagcccg gacggaccgg cgaccggcat gatgatgtcg gcttcctcgg cgatgaagtc
     4081 ctcggccagg cggcgtccgg cgtcctggtc gtcgaacccg atgttcgggt cccccgagat
     4141 gaaggtgccg tcctgcgcct cggggtccca ccccagggcc tcgacctccg tgccgttgtc
     4201 ctggttgtag gcgcgcactc cggccacgaa cccgttcatg aagatcgaca cggtcggcag
     4261 gttggtcccg ccgtacgtgg cgaccttgcc cgactgggtc atgcccgccg ccaggtaccc
     4321 ggccaggaag gcggcctcgt cggtctggaa cgtcagctcc ttgacgttgt cgaacgacac
     4381 gtccgtcggc gggtcggtcg agaagtcgaa gaagttgttg tcgacgatcg cgaagttcct
     4441 gtcggggttg tcctccgccg ccgcctgcgt ggcgtcgccg agcaggaagc cgaccgtgac
     4501 gatcaggtcg cacccctgct gcaggaacgt ctggacgttc ggcgcgtagt ccgcgtcggt
     4561 ctgggactcg agcacgcgct gctcgatgcc gaactccgtt tcggcgtcgg tgaggccctt
     4621 gtacgcggtg gcattgaagc tcttgtcgtc gacgccgccg acgtcggtca cctcacaggc
     4681 aagcgtcccc tccccgatct tggggctggc gctcccgccc ccggacgggg tctcctcggc
     4741 acacgccgcc gcgaggagcg cgagcacgcc gaccagtgcc gccagcatcc gcactcgtct
     4801 ccgcatggtt cctccttccg cgcccgcgcc ctctcgcacg gggttcggcg cattgtacgc
     4861 cgcggccccc ggccgcatag cgaggcaatc agcccccgcc ggcaaggacc tccggctgtc
     4921 acaatgggcc ctcgtggcct ccacggaccc cgccgaccag gcctcggcct cccgggccgc
     4981 gctactgaag gtggtggccc aggcgctcga ggaggacgcg ccgggcggcg atctgacgtc
     5041 ggcgctcacc gtgcccgaag gcgaccgggt ccgcgccgat ctgcgagcga aggcgcccgg
     5101 cgtcgtggcc ggtaccgccg cggccaccgc cgcgttcgac ctgctggcgg cgcgcgacgg
     5161 cggcgggccg gttcgggtgg aatggaacgt ccgcgagggt gactccgtgg cccccgggac
     5221 cgtcctggcc accgtgtccg gctcggcacg cacggtgctc cgcgccgagc gcgtggcgat
     5281 caacttcctg gcgcacctgt cgggcgtcgc gacgctgacg cgcgcgttcg tggaggccgc
     5341 cgccccggcc cgcgtgctct gcacgcgcaa gacgaccccc gggcttcgcg cgctggagcg
     5401 ggaggccgtg ctcgccggtg gcggcgcgct gcaccgcgcc tcgctctccg acgcggtgct
     5461 gatcaaggac aaccacctga aggtcgccgg gggtgtcggc ccggcggtgc gcgccgcgcg
     5521 ggccggcggc gtgccggtcg aggtggaggt cgagacgctc gcgcagctgg atgaggcgct
     5581 cgaggccggc gccgaccgca tcctcctcga caacccgtcg ccggacctgg tgcgccgggc
     5641 ggtcgcgcgc gtgggcgacc cggagcgtct ggaagtctcg ggcggcatta cactcgactc
     5701 ggtgggcgaa ctggtcgcgg cgggcgcccg gatcgtctcc gtcggccggc tgacccattc
     5761 cgcgcccgcg ctggatctct cgctcgaggt gaccgatgtc gaccacgtat gaggagtggg
     5821 ccgccgaggt ccggcggctg gcggcggagc gccgcgcggt catcctcgcc cacaactacc
     5881 aggtcccctg gatccaggac gtcgccgacc acgtcgggga ctccctggcg ctctcgcgtg
     5941 aggcggcgaa gaccgacgcc gagacgatcg tgttctgcgg cgtccacttc atggccgaga
     6001 cggcgaagat cctgtcgccg gagaagaccg tgctgctgcc gaacctggcc gcggggtgct
     6061 cgctggcggc cacgatcgac gcggagcagc tgcgggcctg gaagggggag cacgccggcg
     6121 ccgtcgtcgt ggcctatgtc aacaccagcg ccgaggtgaa ggccgagtcc gacatctgct
     6181 gcacctcgtc gaacgcggcc gagatcgtcc gcacgatccc cgaggaccgc gagatcctgt
     6241 tcctccccga catgttcctc ggcgcgcacg tcgagcgcca gaccggccgg aagctgcacg
     6301 tctggatggg cgagtgccac gtccacgccg ggatcagccc gcaggagctg cgggcgaagg
     6361 ccgagaccga acgcgacgcg gagttcatga tccaccccga gtgcggctgc accacctccg
     6421 ccctgtacct gctggacgag ggcatcctgc cgaaggaccg cacgaaggtg ctgtcgaccg
     6481 gagggatggt ggcggaggcg cagcgctcgg gggcgacgcg gttcctggtg gccaccgaga
     6541 ccggcatcct gcaccagctg cgcaaggccg ctccgggcaa ggtcttcgag ccgatcgacc
     6601 gcaaggccga gtgccagtac atgaaggcga tcacgccgga gaatctcctg cacgcgctgc
     6661 gggagggcgt gtacgagatc acggtgcccg aggagatccg ccgccgcgcc gagcgcgcgg
     6721 tgcagcggat gatcgatctc ggcccggccc cggtccccgc gaccacgtcc tgatccgaac
     6781 gcgccgggcc ccgtgaagac cgtcgtcgtc ggggccgggg ccgccggcct gtgggccgcc
     6841 ctgcacgccg tcgagcaggg tccggtggtc ctggtcgccc ccgacccctc cgccggcagc
     6901 gccaccgcgc tggcacaggg cgggatcgcc gcagccgtgg ccgagggtga cgaccccgct
     6961 gcgcacgccg aggacaccct caccgccggc gcggggctct gcgaccgcga ggcggtccac
     7021 gtcctgaccg ccgaagcgcc cgattcgatc gccgagctgg tgcgccgcgg gatggccttc
     7081 gacgccggag ggcgcccgac gctcgaaggc gggcaccgcg cgcgacgtgt cctgcacgcc
     7141 ggcggcgacg ccaccggccg cttcctgctg caggccctgc tcgcggcggc cgatcgggag
     7201 cggcgcgtcg tctgggatcg cggacgcgtc gcggcgctgc tgcccgaggg aggggcggta
     7261 cgcggggtgc gccgcgccga cggcgcggag gtcgagggcg acaggacgat cctggcgacc
     7321 ggtggcgcct gcgggatcta cggccggcgg accgggccgg accactccac cggtcacggg
     7381 ctggcgctcg cctgggatgc cggcgcggcc ctcgcggacc tggagttcgt gcagttccat
     7441 ccgaccgccc tcgacgtccc gggccacgcg gcgcgcctgc tgaccgaggc gctgcgcggc
     7501 gagggcggtc ggctggtcga tccgcggggc cggcggttca tggccgatca cgacccgcgc
     7561 ggggatctcg cgccgcggga cgtggtatcg cgcgcgatcg ccgccgtgcg cgcggagacc
     7621 gggcgggtgt tccttgacgc cacggggatc gatggcgtcc gcgagcggtt ccccacggcg
     7681 gcggcgacat gcgacgcggt cggcctcgac ctggcgcgcg acccggtccc ggtggcgccg
     7741 gcggcgcact acttcatcgg tggcgtgctc acggacctct ggggccggac gacggtgccg
     7801 ggcctgtcgg cctgcggcga ggtcgcctcg acgggggtcc acggggcgaa ccgactggcc
     7861 tcgaactccc tgctcgagac cctggtgttc gggcggcggg cggcgctcgc cccggaggga
     7921 accggcccgt ccgcggagcc cgaggacccg tacgccgatc ttcccgggac gacggggccc
     7981 tcgctcgagg acgtccgatc ggtcgccgac gccgcgctcg gcgtgcgccg cgatgctccg
     8041 accctcgaag cagcgctccg ctacctggcg acctccgggg gcggcggggc gcacgcgctc
     8101 gtcgcccggc tggtggctcg ggccgcgctg cgccgggagg agtcccgcgg agcccacttc
     8161 cgcgatgacc atccccgcac ggtggaggcc tggcggcgcc ggcaggtcgt gtcgcccctc
     8221 ggctggtgcc ggctgccggt cgcttcctga ccggggcccc gatggggtcc cccaacttcc
     8281 cggaccttcg gcagatgctc ctggcggcgg tctccgtagc ctgaatccgg acatgctgac
     8341 gcgagaagcg gggcatcggg tccgccgcat ccagagcgac gcgttcccgg tgctctcggt
     8401 gtgcctgagc ctcagccccc ttccccgtga cctcgcctcc gtccggccgc ggctgaagga
     8461 cctgctcgac ctgatccgcg ccgccgccga tccggagcgc ctgggacacg aggcggcgat
     8521 gtcggtacgc gccgacgtcg agcgcgtcct ggagctcgag gaccggatcc acgccgacgt
     8581 cggccacggc gtggcggtgt tcgcgtgcca cggcctgggc ccgttcgagt acctcagcct
     8641 gccccgcggg ctgcccgacc gggcctcgct cgacggtcgc gcgcagaccg gcccgttgga
     8701 cgcgttcctg gagcgctccc accggtgcgt ggcggtggtc gtggagcgcg accgctcatc
     8761 gatcttcgag ttccaccagg gcgagctcga ggtgcatcgg gaggtccgcg acgggcgtat
     8821 ccgcaagcgg aactacggcg ggcgggccgg tctcgacgag tacaccgtca cccggcacgc
     8881 gcgggaggag gcgctgcggc accaccggcg cacggtgcgc gcggtcggcc agctcttctc
     8941 ccctcggggg tccgctcgcc ggacgcggtc gagtagagcg t
//
LOCUS       DASPWO010000372         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_395002, whole genome shotgun sequence.
ACCESSION   DASPWO010000372 DASPWO010000000
VERSION     DASPWO010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395002"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VEO00_08535"
     CDS             complement(<1..549)
                     /locus_tag="VEO00_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYU58083.1"
                     /translation="MERVVEPEVSPDERAFDAALRPRRLGEFVGQDRIKEQLALLLDG
                     ARARNEPVDHLLFSGPPGLGKTTLAGIVANEMEVRMHPTSGPALERAGDLAAILTNLD
                     EGDVLFVDEVHRMPRAVEEVLYPALEDFKLDIVVGKGPSARSIRLDLPRFTLVAATTR
                     TGMVTLPLRERFGFSPRLDYYSE"
     gene            complement(597..1169)
                     /gene="ruvA"
                     /locus_tag="VEO00_08540"
     CDS             complement(597..1169)
                     /gene="ruvA"
                     /locus_tag="VEO00_08540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HYU58084.1"
                     /translation="MIASVAGTLVHKGADRVVIEVGGVGLEAVVSRTTLSALPPAGKR
                     VRLFTHLLVRDDALTLVGFADADEREVFTLLLGVTGVGAKVALAILSTLSPDALRRAV
                     VAGDAEAIAVAPGVGKKVAGRIVLDLKDRLGVEGDAVAGGPLAEVREALLALGLSAQE
                     ARDALAALPPNGDRPVEDLLRDALRSVGRP"
     gene            complement(1166..1669)
                     /locus_tag="VEO00_08545"
     CDS             complement(1166..1669)
                     /locus_tag="VEO00_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HYU58085.1"
                     /translation="MFEPVVLGVDPGLAALGVAAVARGNGRPRLLWATTVRTPAGLGD
                     AARLRLVFEALDAALAEHRPEAVAVERLMWGRNSGSAMGVARATGVVMLAAALRGIPL
                     EEYAPLEVKLSVAGAGDATKDQVRRALERLVGMAAVPAAPDAADAVAVAVCHLTQSRL
                     RGLVRRS"
     gene            complement(1738..>2076)
                     /locus_tag="VEO00_08550"
     CDS             complement(1738..>2076)
                     /locus_tag="VEO00_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58086.1"
                     /translation="NVRAGRVVICDRYLLDSEVHLRDRYGRSRRFAVAMWAIRRLSPR
                     PLRAFFLDVPPEVALTRKPDDWTIPELARQAELYREDAARLGVRRLDGTRPPEELCAE
                     IAREVWLALG"
BASE COUNT          292 a          819 c          736 g          229 t
ORIGIN      
        1 ctccgagtag tagtcgagcc gcggggagaa cccgaaccgc tcgcgcagcg ggagggtcac
       61 catccccgtc cgcgtggtgg ccgccacgag cgtgaaccgc ggcaggtcca ggcggatgct
      121 gcgggccgag ggccccttcc cgaccacgat gtcgagcttg aagtcctcga gcgccggata
      181 gagcacctcc tccacggcgc gcggcatgcg gtggacctcg tccacgaaga gcacgtctcc
      241 ctcgtcgagg ttggtcagga tggccgccag gtcaccggcg cgctcgagcg cgggaccgga
      301 ggtgggatgc atccgcacct ccatctcgtt ggccacgatc ccggcgagcg tggtcttgcc
      361 gagccccggg gggccggaga acagcaggtg gtcgaccggc tcgttccggg cgcgggcgcc
      421 gtcgagcagc agggcgagct gctccttgat ccggtcctgc ccgacgaact cccccaggcg
      481 gcgcggccgc agcgccgcgt cgaacgcccg ctcatccggg ctgacctcgg gctcaacgac
      541 gcgctccacg cggaccggct catcctcggg gccgcccgtc cccctgccgc ggaccgtcac
      601 ggccggccca ccgagcgcag cgcgtcgcgc agcaggtcct ccaccggccg gtccccgttc
      661 ggcggcagcg ccgccagcgc gtcgcgggcc tcctgcgccg agagcccgag cgccagcagg
      721 gcctcgcgca cctcggcgag cggtccgccg gcgaccgcat cgccctccac ccccaggcgg
      781 tccttgaggt ccagcacgat ccggccggcg accttcttcc cgactccggg cgccaccgcg
      841 atcgcctccg cgtcgccggc gaccaccgcc cggcgcaggg cgtccggcga cagcgtcgac
      901 aggatggcca gggccacctt cgccccgacg ccggtcacgc cgagcagcaa cgtgaagacc
      961 tcgcgctcgt cggcgtcggc gaacccgacg agcgtcagcg cgtcgtcgcg gaccagcagg
     1021 tgcgtgaaca gccggacgcg cttgccggcc ggagggagcg cggagagcgt cgtgcgcgac
     1081 acgaccgcct cgaggcccac gccgccgacc tcgatcacca cccggtcggc gcccttgtgg
     1141 acgagcgtcc cggccaccga ggcgatcacg accggcgcac caacccccgc aggcgggact
     1201 gggtgaggtg acagacggcg acggccacgg cgtcggccgc gtcgggggcg gccggcacgg
     1261 cggccatccc gaccaggcgc tccagcgccc ggcgcacctg gtccttggtg gcatcccccg
     1321 caccggccac cgacagcttc acctcgaggg gggcgtactc ctccagcggg atcccccgca
     1381 gggcggcggc gagcatcacg acgcccgttg cccgtgcgac gcccatcgcg ctccccgagt
     1441 tgcggcccca catcaggcgc tcgacggcga cggcctccgg acggtgctcc gcgagcgccg
     1501 cgtccagcgc ctcgaacacc agccgcaggc gggccgcgtc gccgagcccg gcgggcgtcc
     1561 gcaccgtggt cgcccacagc aggcgcggac gaccgttccc gcgcgccacg gcggcgaccc
     1621 cgagcgcggc caggcccggg tcgaccccga ggaccaccgg ctcgaacatg tgttcagact
     1681 ctagagagcc cggacgtccg cctcaagcac ctcggccgat tccgctccgg tcggcggtca
     1741 gccgagggcg agccacacct cccgcgcgat ctccgcacac agctcctcag ggggccgggt
     1801 cccgtcgagg cgacggacgc cgagccgggc ggcgtcctcc cgatacagct cggcctgtcg
     1861 tgcgagctcc ggaatcgtcc agtcatccgg cttgcgcgtc agggcgacct cgggcgggac
     1921 gtccaggaag aacgcgcgga gcgggcgcgg cgacaggcgg cggatggccc acatcgcgac
     1981 cgcgaaccga cgggagcgtc cgtaccggtc ccggaggtgg acctcggaat cgaggaggta
     2041 ccggtcgcag atcacgaccc ggccggcgcg gacgtt
//
LOCUS       DASPWO010000373         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_821693, whole genome shotgun sequence.
ACCESSION   DASPWO010000373 DASPWO010000000
VERSION     DASPWO010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821693"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /gene="ccsA"
                     /locus_tag="VEO00_08555"
     CDS             complement(<1..490)
                     /gene="ccsA"
                     /locus_tag="VEO00_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HYU58087.1"
                     /translation="MDAERLSGLSDSAFTAALVGYAIAAAGYIHYLAFRRRPVWVAAR
                     AVAVVSLVAHLGSIVARGLAAGRVPWGNMYEYSSVLAALVVAAYLLIVEWRSKVRTLG
                     GFALGFAVVTMAVARMQFYVVPAPLQPALNSSWLKVHVISAIVGSSLLTLGSLFTILF
                     LVQ"
     gene            complement(480..2009)
                     /locus_tag="VEO00_08560"
     CDS             complement(480..2009)
                     /locus_tag="VEO00_08560"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HYU58088.1"
                     /translation="MERARRPLPTFRESLAFVWRSLRSMRTALVLLLLVGAASVAGSL
                     VPQTGVSEDRIAALVRDHPLRAAIFRRLGLFDVYGSWWFTTLYALLLVSLATCLLPRT
                     RALARNLRARPQFAQDLVGLRHHAERTLPGEPAEALSRARRVLRRRRFRVAAGRNGGG
                     PMLAAEKGMAREIGSLAFHWAFFLILVGAVYGKATGFTGRAVIIEGSTWTETHAAYDG
                     NIREGSLFREDHSGVGVHVRDFEATYRDDGTPKDFVTHADLLDADGNLVRSQDIRVNH
                     PAELDHVKLYQFGYGWAPVIEVRADGRTVASSPVVFVRDQVEGVDQNALPWRGVLRLP
                     SLRPQVGLEFLLWPDRRALERFLLTGVSSPMLRPFEPVLLVTAYRGDLRLTASQNPTR
                     LDTSGMERFLANAPVAEGRALDVVHGLTLSFPELREYTVLQVTRDRGVPIMLVAAVMI
                     LVGLLPALYGSRRKVWVRAEPAGEGTRLEVGGFALQRKAQFEEEFARLVDDLGAAPGG
                     R"
     gene            complement(2020..>2367)
                     /locus_tag="VEO00_08565"
     CDS             complement(2020..>2367)
                     /locus_tag="VEO00_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HYU58089.1"
                     /translation="TPPHFWALALRFSGQYAKAGVPMLPVVAGVRRTTRQIVGYSVLV
                     VATSLALVPAAGMGPVYWISALVLGAWFVAGAVRLQRRDGRAPMRLFRDSITYLALLF
                     AAVAVDTLIRFGP"
BASE COUNT          393 a          859 c          854 g          261 t
ORIGIN      
        1 cctggaccag gaacaggatc gtgaacaggc tccccagcgt gaggagcgac gacccgacga
       61 tcgcggagat gacgtggacc ttgagccagg aggagttcag ggccggctgc aggggcgcgg
      121 ggaccacgta gaactgcatc cgggccacgg ccatcgtcac gacggcgaac ccgagggcga
      181 acccaccgag cgtgcggacc ttcgatcgcc actccacgat cagcaggtag gcggccacca
      241 cgagggcggc cagcaccgac gagtactcgt acatgtttcc ccacggcact cggccggcgg
      301 ccagcccccg ggccacgatc gagccgagat gcgcgacgag ggagacgacc gcgacggcgc
      361 gtgccgccac ccagaccggg cgccggcgga aggccaggta gtggatatag ccggccgcgg
      421 cgatcgcgta gcccaccagc gcggccgtga acgccgagtc ggacagcccc gagagccgct
      481 cagcgtccac cgggcgccgc tcccaggtcg tccaccaggc gcgcgaactc ctcctcgaac
      541 tgcgccttgc gctgcagggc gaacccccca acctcgagcc gggtgccctc cccggccggc
      601 tctgcgcgga cccagacctt gcggcgggag ccgtagagcg ccggcagcag gccgacgagg
      661 atcatgaccg cggccaccag cataatcggg accccccggt cgcgcgtcac ctgcagcacg
      721 gtgtactcgc gcagctcggg gaacgagagc gtcaggccgt gcaccacgtc gagcgcccgt
      781 ccctcggcca ccggcgcgtt ggccaggaac cgctccatcc cggaggtgtc cagtcgcgtc
      841 gggttctgcg aggccgtcag gcgcaggtcg ccgcggtagg cggtcaccag cagcaccggc
      901 tcgaacggcc ggagcatggg agaggacacg ccggtgagga ggaaccgctc cagcgcccgg
      961 cggtcgggcc acaacaggaa ctccagcccg acctgcgggc ggagcgacgg cagccggagc
     1021 accccccgcc aggggagggc gttctggtcc acgccttcga cctggtcgcg gacgaacact
     1081 accgggctcg aggcgaccgt ccggccgtcc gcgcggacct cgatcaccgg cgcccagccg
     1141 tacccgaact gatagagctt gacgtggtcc agctccgcgg gatggttcac gcggatgtcc
     1201 tgggagcgga ccaggttccc gtcggcgtcg agcaggtccg cgtgcgtgac gaagtccttc
     1261 ggcgtgccgt cgtcgcggta cgtcgcctcg aagtcgcgca cgtgcacgcc gaccccggag
     1321 tggtcctcgc ggaacagcga tccctcgcgg atgttgccgt cgtaggccgc gtgggtctcg
     1381 gtccaggtgc ttccctcgat gatcaccgcc cgtccggtga agccggtggc cttcccgtac
     1441 acggcgccga ccaggatcag aaagaacgcc cagtggaagg cgaggctgcc gatctcccga
     1501 gccatccctt tctcggccgc gagcatcggc ccacctccgt tccttccggc tgcgacccgg
     1561 aagcgccgcc ggcgcagcac gcggcgggcc cgggagagcg cctcggccgg ctcgccgggg
     1621 agggtccgct ccgcgtggtg gcgcagcccg actaggtcct gggcgaactg cggccgggcg
     1681 cgcaggttcc gcgccagggc ccgcgtccgg ggcagcaggc acgtcgccag ggagaccagc
     1741 agcagcgcgt agagcgtggt gaaccaccac gatccgtaga cgtcgaagag cccgagccgg
     1801 cggaagatcg ccgcgcgcag cgggtgatcc cggacgagcg ccgcgatgcg atcttccgag
     1861 acgccggtct gggggacgag ggagccggcc accgacgccg ccccgaccag cagcaggagg
     1921 acgagtgccg tgcgcatcga gcgcaggctg cgccagacga acgccagcga ctcccggaac
     1981 gtggggaggg gacggcgcgc gcgctccacg gcttcggcgc tacgggccga accggatcag
     2041 cgtgtccacg gcgaccgcgg cgaagagcag cgcgaggtag gtgatggagt cccggaacag
     2101 cctcatcggg gcgcgaccat cgcggcgctg cagccggacc gcgccggcga cgaaccaggc
     2161 gccgagcacg agcgcgctga tccagtacac cggtcccatc ccggcggcgg gcacgagcgc
     2221 caggctcgtg gcgaccacca gcaccgagta gcccacgatc tgccgcgtcg tgcggcgcac
     2281 tccggccacc accggcagca tcgggacgcc cgccttcgcg tactggccgg agaagcgcag
     2341 ggcgagcgcc cagaagtgcg gcggcgt
//
LOCUS       DASPWO010000374         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_311450, whole genome shotgun sequence.
ACCESSION   DASPWO010000374 DASPWO010000000
VERSION     DASPWO010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311450"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VEO00_08570"
     CDS             <1..616
                     /locus_tag="VEO00_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYU58090.1"
                     /translation="INQAYEIQRRLRVWQPRRAAVLGAGTIGLLATMALRVRGLEVVT
                     MGLQEAPYLNSDLVEALGARYVSTSERTIPQIAADHGQFDLIIEATGFSPIVFEAMCL
                     LLGKNGVIVLSSVTGGSRRVDVPADAINLDFVLGNKVMVGTVNANREHFEAGVRDFAL
                     AEARFPGWLARLLTHPVPGLQEWPRAFELLGAPGTIKVFIEVSP"
     gene            627..1189
                     /locus_tag="VEO00_08575"
                     /pseudo
     CDS             627..1189
                     /locus_tag="VEO00_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415117.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            1258..>2079
                     /locus_tag="VEO00_08580"
     CDS             1258..>2079
                     /locus_tag="VEO00_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58091.1"
                     /translation="MAVATGTSEQLGTGLAFARLEEWRAVEVTGGDAFDWLNDLVTAD
                     LAPLAAASGPGTTCPALRSLLLTPTGGVRATFTVAAIGASLLLLQDPAEPRAIGDLLG
                     PYVLSSDVTLTDRTGALTILARPGNPGSTSAEEGTLLEPSVLSPSGSAPGLDVLVPAG
                     AADAAVAAFRETLAMAPAEEVEAWRVAAAIPRAGVDVLEADLPVEAGLDRAVAVAKGC
                     YLGQEAVARTRNLGRPRRSLVAVSADGPVAPGESVLTDDEEVGAITSAASFAGSTV"
BASE COUNT          260 a          741 c          780 g          298 t
ORIGIN      
        1 gatcaaccag gcctacgaga tccagcgtcg cctgcgcgtg tggcagccgc ggcgggccgc
       61 ggtcctcggc gcggggacga tcggtttgct cgccacgatg gccctgcgcg tccgcgggct
      121 ggaggtggtg acgatggggc tgcaggaggc gccgtacctc aattcggacc tcgtcgaggc
      181 gctcggcgcg cgctacgtga gcacgagcga acggaccatc cctcagatcg ccgccgacca
      241 cggacagttc gacctgatca tcgaggccac cggcttctcg ccgatcgtct tcgaggccat
      301 gtgcctgctg ctcggcaaga acggcgtgat cgtgctctcg agcgtcacgg gcggctcgcg
      361 ccgggtggac gtccccgccg acgccatcaa cctcgacttc gtcctcggga acaaggtgat
      421 ggtcgggacc gtcaacgcca atcgcgagca cttcgaggcc ggggtccggg acttcgcgct
      481 ggccgaggcc cgcttcccgg ggtggctcgc ccgcctgctg acccaccccg tccccgggct
      541 ccaggaatgg ccgagggcgt tcgagctcct cggcgcgccc ggcacgatca aggtcttcat
      601 cgaggtctcg ccctaggctg gccgccgtgc cggcgggaca agggttcctg gtcgacctcg
      661 atgggacgct cgtcgacagc gtctaccagc acgtgctggc ctggcaggag gcgctcgacc
      721 agctcggcat cgcgatctcc gtctggcgca tccatcgcaa gatcgggatg agcggcgggc
      781 tgttcgcgaa cgcgctgctg cgggagaccg ggcgcgcggt cggcggcgag gaggcggccc
      841 ggctgcgcgc gctccacgcg gaggcgtacg gccggcgcgt cgaccaggtg cggccgcttc
      901 cgggatcgcg ggagctgttg tcgttcctgc agagcgcgga cgagccgtgg gccatcgcca
      961 cgagcggcta tctcgagtcg agggatcggt cgtcatcggt gacagcgtgt gggacctgct
     1021 ggcggcacga cgggcgcgcg cgctctgagt ggggctgctc tcgggcggct acggccagga
     1081 cgagctggag cgcgccggcg cctaccgcgt gtacgaggac cccgccgatc tcctgcggca
     1141 cctcgacgag gtcggcctga gggtgcacgg ccacgacgcc ggcgcctgag tggcgccttc
     1201 cgtccagaga ccacgctcgc ctcgagcggc ttcgcgggac ggggctatcc tcggaccgtg
     1261 gcggtcgcga ccggaacgtc ggagcagctc gggaccggac tcgccttcgc ccgcctcgag
     1321 gagtggcggg cggtcgaggt gaccggcggc gacgctttcg attggctgaa cgacctggtc
     1381 accgccgacc tcgcaccgct ggcggccgcc tcgggccccg gaacgacgtg cccggcactg
     1441 cggtcgctgc tgctcacccc aaccggcggg gtgcgggcca cgttcaccgt cgccgccatc
     1501 ggcgcgagcc tcctcctgct gcaggacccg gccgagccgc gcgcgatcgg cgacctgctc
     1561 ggcccgtacg tcctgtcgtc cgacgtcacg ctgaccgacc ggaccggcgc gctgacgatc
     1621 ctggcacggc cgggcaaccc cggctccacc tccgccgagg agggaacgct tctggaaccg
     1681 tcggtgctct ctccgtcagg gtccgcgccc gggctggacg ttctcgtccc ggccggcgcc
     1741 gccgacgcgg cggtcgccgc gttccgggag accctcgcga tggcgccggc cgaggaggtc
     1801 gaggcatggc gggtggccgc cgccatccct cgcgcagggg tcgacgtgct ggaggccgac
     1861 ctgccggtgg aggcgggcct ggaccgggcc gtcgccgtgg cgaagggatg ctacctgggg
     1921 caggaggccg tggcccgcac gcgcaacctc ggccggccgc ggcggtcgtt ggtggcggtt
     1981 tcggccgacg ggcccgtcgc cccgggggaa tcggtcctca cggacgacga ggaggtgggc
     2041 gcgatcacca gcgccgcatc gttcgccggg tcgacggtc
//
LOCUS       DASPWO010000375         4105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_646775, whole genome shotgun sequence.
ACCESSION   DASPWO010000375 DASPWO010000000
VERSION     DASPWO010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646775"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1640
                     /locus_tag="VEO00_08585"
     CDS             96..1640
                     /locus_tag="VEO00_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYU58092.1"
                     /translation="MVEGGFDAIVVGAGHNGLVTAALLGMRGLRVCVLERRDVVGGAC
                     VTEELYPGFKISTAAYSFSLFRPEVVQELDLLRHGLSFYPKDPQMFVPLPDGRSFFVW
                     RDRDRTLEELRRIHPSDADAYHRWEAFWDEAAELIRPMLLSEPPSLADLEKRLDRRGK
                     SELFRLAIAGSAEETVAEFFESDELRGAYASQGIIGTAAGPRTPGTAFVMTYHAFGGE
                     LVDDNGAWAYVRGGMGGVTQALLSAAREHGVDVRTGAPVAEVLVDDGAVGGVRLEDGT
                     EVHAPAVLSNADPKRTFLGIVPDGALPGPFMDRVRTFDMTGSVVKVNMALSDLPDFTS
                     RPGTEPGPQHAGTIEISPSVDYLEAAHADAVAGRVSSRPFMELFIQSVTDPTLAPEGR
                     HVASAFAQYVPGSVPMEEWEGMREAAGDAVIDALAVFAPNVRDTIIHREVLGPADLEA
                     RLGLTGGNIFHGEILPGQVFGERFGPRTPIAGLYLCGSGAHPGGGVTGAPGWNAAHAL
                     LADRGA"
     gene            complement(1652..2569)
                     /locus_tag="VEO00_08590"
     CDS             complement(1652..2569)
                     /locus_tag="VEO00_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYU58093.1"
                     /translation="MADPESGLGTEAPAEERCYRHPNEVTRVHCTRCGRPICPDCMHP
                     APVGHHCPTCVGEARREARVGPGRRARVMLGRPGGVTTLLLGAILAMFAVEVVVGGPQ
                     SLMSGPPVQKLIRLGATAPSLIAGGQWWRLITATFLHAGILHLLLNGYALYLLGFLVE
                     GVYGKGRFLAIYLITGFLASVASYQFSGLNTVGVGASGAIFGLLGTWLAYNFRRRELA
                     TAAANLRWVLTILLINLVLGFSIRGIDNSAHIGGFLAGILAGTLAEGFGRGPARRLVQ
                     VGGLIALVLLGVVVTMARTAAIHDMVGLG"
     gene            complement(2634..3401)
                     /locus_tag="VEO00_08595"
     CDS             complement(2634..3401)
                     /locus_tag="VEO00_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58094.1"
                     /translation="MAHPLDGLRRVAADRTWGASELVAAAAEEFAALSPDYRFEGIEI
                     LLGGHPEMAPLWHLCNEVLEGRSGDFAALLAAETAAIALTAAPLLHGIVLTHSYSGTA
                     VRSIVRAAPMKVLCTHSEPGGEGRMTAEALRAAGLDVEVVADDAVGEVDFQVAVIGAD
                     AVTPAGVTNKVGTAAVADAARARGAMSLVLAGAAKLMPVPLPVPEPFEMVPITAFTAI
                     VTEDGVHGEEEVLAEAAQVKLNEALIRRAARAVDGTT"
     gene            complement(3492..3962)
                     /locus_tag="VEO00_08600"
     CDS             complement(3492..3962)
                     /locus_tag="VEO00_08600"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HYU58095.1"
                     /translation="MAFESTRRGGSHDGHEARHAAEVDNAWITEVLSADGYTVRPGTQ
                     DVNNILAEHPSRPNVTIRVHPELGLITIVQLWGLKKGGGFGAHKNLMEKLNKANSQSW
                     RDTYYITTEGDLGVSSYITLADQLSAEDITAHLARESEGFFTTVTVAGLREHLA"
BASE COUNT          561 a         1485 c         1467 g          592 t
ORIGIN      
        1 cccgaaccga ccccccgtac accgtcgagc agctgggccg tctcgtccag gccgtcgacg
       61 agaccatccg ggcgagggac tagcatcccg ccaggatggt ggaggggggg ttcgacgcga
      121 tcgttgtcgg ggccgggcac aacggcctgg tcacggcagc cctgctcggg atgcgcggcc
      181 tgcgcgtctg cgttctggag cggcgcgacg tggtcggcgg cgcgtgcgtg accgaggagc
      241 tgtacccggg gttcaagatc tccacggccg cctactcatt cagcctgttc cggcccgaag
      301 tggtgcagga acttgacctt ctccggcacg ggctgtcgtt ctatccgaag gacccgcaga
      361 tgttcgtgcc gctgccggac gggcggtcct tcttcgtctg gcgcgaccgt gaccgcacgc
      421 tcgaggagct gcgccggatc cacccgtccg acgccgacgc gtaccatcgc tgggaggcat
      481 tctgggacga ggcggccgag ctcatccggc ccatgctgct gtccgagccg ccctcgctcg
      541 ccgacctcga gaagcggctg gaccgccggg ggaagtccga gctcttccgg ctggcgatcg
      601 ccggctcggc cgaggagacg gtggccgagt tcttcgagag cgatgagctg cgcggcgcgt
      661 acgccagcca ggggatcatc ggcacggccg ccggcccgcg cacgccgggc acggcattcg
      721 tgatgaccta ccacgcgttc ggcggtgagc tggtggacga caacggcgcg tgggcctacg
      781 tgcgcggcgg gatgggcggc gtgacccagg cgctgctctc ggcggcccgc gagcacggcg
      841 tcgacgtgcg caccggtgca ccggtcgccg aggtcctggt cgacgacggc gcggtggggg
      901 gcgtacgcct ggaggacggg accgaggtgc acgccccggc ggtgctgtcg aacgccgacc
      961 cgaagcggac gttcctgggc atcgtccccg acggcgcgct gccggggccc ttcatggacc
     1021 gcgtccggac cttcgacatg accggctcgg tggtcaaggt gaacatggcg ctctcggatc
     1081 tccccgactt cacgtcgcgc ccgggcacgg agccgggccc gcagcacgcc ggcacgatcg
     1141 agatctcacc gtcggtggac tacctggagg cggcgcacgc cgacgcggtg gccggccggg
     1201 tgtcgtcacg tccgttcatg gagctgttca tccagtcggt gaccgacccg acgctggcgc
     1261 cggaaggacg gcatgtcgcg tcggccttcg cgcagtacgt gcccggctcg gtgccgatgg
     1321 aggagtggga ggggatgcgc gaggcggccg gcgacgcggt gatcgacgcc ctggccgtct
     1381 tcgccccgaa cgtccgcgac acgatcatcc accgggaggt gctcgggccg gccgacctcg
     1441 aggcgcgcct cggcctgacc ggcgggaaca tcttccacgg ggagatcctg ccggggcagg
     1501 tcttcggcga gcgcttcggc ccgcggaccc cgatcgccgg gctctacctc tgcggctccg
     1561 gcgcccaccc cggcggcggc gtcaccggcg cgccgggctg gaacgccgcc cacgcgttgc
     1621 tcgccgaccg cggcgcctga cgagctcctc gctagccgag ccccaccatg tcgtggatgg
     1681 cggcggtacg ggccatcgtc accacgaccc ccaggagcac cagcgcgatc aagccgccga
     1741 cctggaccag gcgccgggcc gggccgcggc cgaagccctc ggccagggtg ccggccagga
     1801 tgccggcgag gaatccgccg atgtgcgccg agttgtcgat gccgcggatc gagaagccga
     1861 ggaccaggtt gatcagcagg atcgtcagca cccagcgcag gttggcggcg gccgtggcca
     1921 gctcgcggcg gcggaagttg taggccagcc acgtcccgag cagcccgaag atggcgcccg
     1981 acgcgccgac ccccaccgtg ttcagcccgc tgaactgata cgaggccacg ctcgccagga
     2041 acccggtgat cagatagatc gccaggaagc gccccttgcc gtacacgccc tcgaccagga
     2101 agccgagcag gtagagggcg tagccgttca gcaggagatg caggattccg gcgtgcagga
     2161 acgtcgccgt gatcaggcgc caccactggc cgccggcgat caggctcggg gcggtcgccc
     2221 cgagcctgat cagcttctgg accggcggcc cgctcatcag cgactgcgga ccgccgacca
     2281 ccacctccac cgcgaacatc gcgaggatcg cgccgagcag cagcgtggtc acgccgcccg
     2341 ggcggccgag catgacgcgg gccctgcggc ccggtccgac gcgggcctcg cggcgggcct
     2401 cgccgacgca ggtcggacag tggtggccga ccggggcagg atgcatgcag tcggggcaga
     2461 tcgggcggcc gcatcgcgtg cagtgcacgc gcgtcacctc gttcgggtgg cggtagcagc
     2521 gctcctcggc gggggcttcg gtccccaggc cgctctcggg atcggccatg cgcggccatc
     2581 gtagcaacgg gtcccgtttt cccctggggc gttcgctcgc tgcggtgagg ccgtcaggtc
     2641 gtgccgtcga ccgcacgtgc cgcgcggcgg atgagggcct cgttcagctt gacctgggcg
     2701 gcctcggcca ggacctcctc ctctccgtgg acgccgtcct ccgtgacgat cgcggtgaag
     2761 gccgtgatcg ggaccatctc gaagggctcg ggaaccggga gcggaaccgg catcagcttc
     2821 gccgccccgg ccagcaccag gctcatcgcg ccgcgggccc gggctgcgtc ggccaccgcg
     2881 gccgtgccga ccttgttcgt cacgccggcc ggcgtgacgg cgtcggcgcc gatcaccgcc
     2941 acctggaagt cgacctcgcc gaccgcgtcg tccgcgacca cctcgacgtc caggccggcc
     3001 gcccgcaggg cctcggccgt catgcggccc tcgccgcccg gctcggagtg cgtgcagagc
     3061 accttcatcg gggcggcgcg cacgatcgag cgcacggccg tcccgctgta ggagtgggtc
     3121 aggacgattc cgtggagcaa cggcgccgcg gtcagggcga tcgccgccgt ctccgcggcg
     3181 agcagcgcgg cgaagtcccc cgagcgaccc tcgagcacct cgttgcacag gtgccagagc
     3241 ggcgccatct ccgggtgccc cccgagaagg atctcgatgc cctcgaaccg gtagtcgggg
     3301 gagagcgccg cgaactcttc cgcggcggcg gcgaccagct cgctcgcgcc ccaggtgcgg
     3361 tcggccgcga cgcggcgcaa tccgtcgagc gggtgcgcca tcgccggtca gcgtaacgcg
     3421 gctgccacgg acacgacagc gagcccgaac ggcggcgcat accgccgctc gggctcggag
     3481 tgcggcctcg cctaggccag gtgctcccgc aatcccgcca cggtgaccgt ggtgaagaac
     3541 ccctcgctct cgcgcgccag gtgggcggtg atgtcctccg cggacagctg gtccgccagc
     3601 gtgatgtacg agctgacccc caggtcaccc tcggtggtga tgtagtacgt gtcccgccag
     3661 gactggctgt tcgccttgtt cagcttctcc atcaggttct tgtgcgcgcc gaagccgccg
     3721 cccttcttca gaccccagag ctgcacgatg gtgatcagcc cgagctccgg gtgcaccctg
     3781 atggtgacgt tgggccggct tgggtgctcc gccaggatgt tgttcacgtc ctgggtcccc
     3841 ggtcgaacgg tgtatccgtc cgcgctcagg acctccgtga tccacgcgtt gtcgacctcg
     3901 gcggcgtgcc gtgcttcatg accgtcatgg ctccctcctc ttcgagtgct ttcgaatgct
     3961 atcgacgtct tccggcggac actccctcct gcctccccgc gccgacggta ccggagcgat
     4021 cagccccggg agatgtgccg aaggtcccgg tcctgccggg acccccatcg tctacgtcca
     4081 gccgagcgct ccggccgcga ggccc
//
LOCUS       DASPWO010000376         4772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_311720, whole genome shotgun sequence.
ACCESSION   DASPWO010000376 DASPWO010000000
VERSION     DASPWO010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311720"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VEO00_08605"
     CDS             complement(<1..633)
                     /locus_tag="VEO00_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYU58096.1"
                     /translation="MREIGGDPVSVIGYGAWEVGGDAWGPNPDEDEVIGAIRSALDAG
                     MTWIDTAEIYGNGRSEELVGKAIAGREDVFVATKVGESRSGYHAEGVRRGAEGSLKRL
                     GRDAIDLYQVHWPGDRVPLAETWEAMARLVDDGLVRHIGLSNFARRDIERCEPIRHVD
                     SLQNQFSMLYREGGDDLYPWCAENGTAVFAYGPLAFGLLTGAIGMDTEFDE"
     gene            complement(643..1815)
                     /locus_tag="VEO00_08610"
     CDS             complement(643..1815)
                     /locus_tag="VEO00_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HYU58097.1"
                     /translation="MPRSIYLDHASSMPLRPAARAAMLEALDAVGDPHRPHRDGREAR
                     RLLERARERVAAVLAAQPEEIVFTSGGTESVGLALLGAARANRDGGTRAVTTAVEHAS
                     VLGAGQRLRDDGFEVAEVGVDEHGRADLDRFLAEVSRPGTILASVQHANNEVGTMQPL
                     AEAARLARQHGALIHTDACQTTGRLPVDVGALGVDLLSLSGHKFGGPPGAGALYVRRG
                     VDLAPVLGGDDRERGLRPGRENLPAIVGMAAALEAAAAELAEEAGRLWALTDRLRAGL
                     EERVEGVQVHGHPTQRVPHIVCWSVLGLDAEPLLMALDERGFRLDAGSTCTGEAQEPS
                     HVLAAMGRVTTGSLRASVGPGNTEADVDALLEALPEVVGGLRQVASTTAKTNGDGG"
     gene            1866..2069
                     /locus_tag="VEO00_08615"
     CDS             1866..2069
                     /locus_tag="VEO00_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58098.1"
                     /translation="MKPKDVPDPSDSTAWETVSKADDPDNEVRAREQAEQILAESEER
                     TEHPSTTDLEDDTIERRHSDETA"
     gene            2160..4040
                     /locus_tag="VEO00_08620"
     CDS             2160..4040
                     /locus_tag="VEO00_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016640205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HYU58099.1"
                     /translation="MATTETDAQADQRPRKAVKELERVVIRFAGDSGDGMQLTGDRFT
                     SASAAFGNDVATLPDYPAEIRAPAGTVAGVSSFQVHFADYDILTPGDAPSVLVAMNPA
                     ALKKNAGDLIVGGTLIVNVDAFNESNLKKAGYGADPLKDGSLDRFQVVEVPLTSLTLE
                     ALKEFPISKKEAERAKNMFALGIVCWMYHRPPETTIEWIEKKFAKRQEIAGANVTAFK
                     KGIDFGETTELFTSSYKVRPATMPPGTYRNITGNQALAYGLIAAAQQAKLPLFLGSYP
                     ITPASDVLHELSRHKAFDVRTFQAEDEIAAVGAALGAAFAGSLAVTTTSGPGVDLKAE
                     TVGLAVSVELPLLIIDIQRGGPSTGLPTKTEQADLLHVMFGRHGEAPVPIVAAYAPSH
                     CFDAAIEAARIALKYRTPVYLLSDGYLGNGAEPWLVPNVGELPDIAVPFATGPNHDGE
                     FWPFLRDERTLARPWAVPGTAGLEHRIGGLEKSDGTGNISYDPENHELMTRLRAEKIA
                     RIADDIPPLEVDEDTGAEVLVLGWGSTYGVILAACRRLRARQRKVARAHLMHLNPFPR
                     NLGEVLKRYRKVVIPEINLGQLRKLIRAEFLVDAIGYNRVRGLPFRAADLEEALLEVI
                     DG"
     gene            4033..>4772
                     /locus_tag="VEO00_08625"
     CDS             4033..>4772
                     /locus_tag="VEO00_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010475926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HYU58100.1"
                     /translation="MAESGENVSGNGAGAADDGQGVATLTRKDFQSDQEVRWCPGCGD
                     YAVLSAVQGVMPELGIPRERIVFVSGIGCSSRFPYYLNTYGMHGIHGRAPAIATGIST
                     SRPELSVWVATGDGDGLSIGGNHLIHAMRRNVNLRILLFNNEIYGLTKGQYSPTSAAG
                     KVTKSTPFGSIDWPFNPVSLALGAECTFVARALDVDKAHLTEIMKRAAAHRGTAFVEI
                     YQNCPVFNDDQFIALTGKDQKALNQIHL"
BASE COUNT          746 a         1754 c         1643 g          629 t
ORIGIN      
        1 ctcgtcgaac tcggtgtcca tgccgatcgc cccggtcagc aggccgaagg cgagcgggcc
       61 gtaggcgaac accgccgtgc cgttctccgc acaccacggg tagaggtcgt cgcccccctc
      121 gcggtagagc atcgagaact ggttctgcag cgaatcgacg tggcggatcg gctcgcaacg
      181 ctcgatgtcc cggcgcgcga agttcgatag gccgatgtgg cggaccaggc cgtcatcgac
      241 caaccgggcc atcgcctccc aggtctcggc gagcgggacc cgatcgcccg gccagtgcac
      301 ctggtagagg tcgatcgcat cacgaccgag ccgcttcagc gagccctcgg cgccgcggcg
      361 gacgccctcc gcgtggtacc cggagcggct ctcgccgacc ttcgtcgcga cgaagacgtc
      421 ctcgcggccg gcgatcgcct tcccgacgag ctcctcggag cgcccgttcc cgtagatctc
      481 ggcggtgtcg atccaggtca tcccggcgtc gagcgcggac cggatggccc cgatcacctc
      541 gtcctcgtcc gggttcgggc cccaggcgtc cccgcccacc tcccaggcgc cgtacccgat
      601 caccgacacg gggtcccccc cgatctcgcg catctgcacc ggctaccccc cgtctccgtt
      661 cgtcttcgcc gtcgtcgacg cgacctgccg aagccccccg accacttcgg ggagcgcctc
      721 gagcagggca tcgacgtcgg cctccgtgtt gcccggcccg acgctcgccc ggagcgaccc
      781 ggtggtgacc ctgcccatcg ccgccaggac gtgcgacggc tcctgggcct ctccggtgca
      841 ggtcgagccg gcgtcgagcc ggaacccgcg ctcgtcgagc gccatcagca gcggctcggc
      901 gtccaggccg aggacgctcc agcagacgat gtgcgggaca cgctgcgtgg ggtgcccgtg
      961 cacctggacg ccctccaccc gctcctcgag gcccgcgcgc agccggtcgg tcagcgccca
     1021 cagccggccg gcctcctcgg cgagctccgc ggccgcggcc tcgagcgccg cggccatccc
     1081 gacgatcgcc ggaaggttct cccggccggg ccgcagcccc cgctctcggt cgtccccgcc
     1141 gagcaccggc gcgaggtcca cgccgcggcg aacgtagagc gcgccggcgc cgggagggcc
     1201 gccgaacttg tgccccgaca gcgacaggag gtcgacgccg agggccccga cgtcgaccgg
     1261 cagccggcca gtcgtctggc aggcgtcggt gtggatcagc gccccgtgct ggcgggcgag
     1321 gcgggcggcc tcggcgagcg gctgcatcgt gccgacctcg ttgttcgcgt gctggaccga
     1381 ggccaggatc gtccccggcc gcgagacctc ggccaggaac cggtcgaggt cggcacggcc
     1441 gtgctcgtcg acacccacct cggcgacctc gaagccgtca tcgcgcaggc gctgaccggc
     1501 cccgaggacg ctagcgtgct ctacggcggt ggtcacggcc cgggtcccgc catcgcggtt
     1561 cgcccgggcg gctccgagca acgcgagccc gaccgactcg gtcccgccgc tggtgaagac
     1621 gatctcctcg ggctgggccg ccagaacggc ggcgacccgc tcgcgggccc gctccaggag
     1681 gcggcgagcc tcgcggccgt cccggtgcgg gcggtgcgga tcgccgaccg cgtcgagggc
     1741 ctcgagcatc gccgcccggg ccgcgggccg cagcggcatc gaggaggcgt gatcgaggta
     1801 gatcgagcgc ggcatggttt catagagtgg cacaaacggg gaaaagagcc tcagggaggg
     1861 aaaccgtgaa gccgaaggac gtacccgacc cctcggacag caccgcctgg gagaccgtgt
     1921 cgaaggccga cgaccccgac aatgaggtgc gggcccggga gcaggccgag cagatcctgg
     1981 cggagtcgga ggaacggacc gagcatccct cgaccaccga cctcgaggac gacaccatcg
     2041 agcgccgtca ctcggacgag acggcctgag gccgcctcgc cgcgccgcac cacgcggcgg
     2101 cggccggggg cgcatcggcg ccccgcacgt acaatggcac aaggagatct gagagccaca
     2161 tggcaaccac cgaaaccgac gcccaagccg atcagcgtcc caggaaggcc gtcaaggagc
     2221 tcgagcgtgt cgtcatccgc ttcgcgggtg actccggcga tggcatgcag ctcaccggcg
     2281 accgcttcac cagcgccagc gccgccttcg gcaacgacgt ggcgacgctc ccggactacc
     2341 cggcggagat ccgtgccccg gccggcacgg tggccggcgt ctcctcgttc caggtccact
     2401 tcgccgacta cgacatcctg acccccgggg acgccccgag cgtcctggtg gcgatgaacc
     2461 cggcggcgct gaagaagaac gccggggacc tgatcgtcgg cgggaccctg atcgtgaacg
     2521 tcgacgcgtt caacgagtcg aacctgaaga aggccggcta cggggcggat ccgctgaagg
     2581 acggctcgct cgaccggttc caggtcgtcg aggtgccgct gacgtcgctg acgctcgagg
     2641 cgctgaagga gttcccgatc tcgaagaagg aggccgagcg ggcgaagaac atgttcgccc
     2701 tgggcatcgt ctgctggatg taccaccggc cgcccgagac gacgatcgag tggatcgaga
     2761 agaagttcgc caagcgccag gagatcgccg gggcgaacgt caccgcgttc aagaagggca
     2821 tcgacttcgg cgagacgacc gagctgttca cctcgagcta caaggtccgc ccggccacga
     2881 tgccgcccgg cacgtaccgc aacatcaccg ggaatcaggc gctggcctac ggcctgatcg
     2941 cggcggcgca gcaggcgaag ctgccgctgt tcctgggctc gtatccgatc acgccggcct
     3001 cggacgtgct gcacgagctc tcccgccaca aggcgttcga cgtccggacg ttccaggccg
     3061 aggacgagat cgcggcggtc ggcgcggccc tcggcgccgc gttcgccggc tcgctcgcgg
     3121 tgaccaccac ctccggtccg ggtgtcgacc tgaaggcgga gaccgtcggg ctggcggtca
     3181 gcgtcgagct gccgctcttg atcatcgaca tccagcgcgg cggcccctcc accggcctgc
     3241 cgacgaagac cgagcaggcc gacctgctgc acgtgatgtt cgggcgccac ggcgaggccc
     3301 ccgtgccgat cgtcgccgcc tacgcgccgt cgcactgctt cgacgcggcg atcgaagcgg
     3361 cccgcatcgc attgaagtac cgcaccccgg tgtacctgct ctccgacggg tacctcggca
     3421 acggcgcgga gccgtggctg gtgccgaacg tgggcgagct gcccgacatc gcggtgccgt
     3481 tcgccaccgg gccgaaccac gacggcgagt tctggccgtt cctgcgcgac gagcgaacgc
     3541 tcgcccgccc gtgggccgtt cccggcacgg ccggcctgga gcaccgcatc ggcgggctgg
     3601 agaagtccga cgggaccggc aacatctcct acgacccgga gaaccacgag ctgatgacgc
     3661 ggctgcgcgc cgagaagatc gcgcggatcg ccgacgacat cccgccgctc gaggtggacg
     3721 aggacaccgg ggccgaggtg ctcgtcctcg gctggggctc gacctacggg gtgatcctcg
     3781 ccgcgtgccg ccggctgcgg gcgcgccagc gcaaggtggc ccgggcgcac ctgatgcacc
     3841 tgaacccgtt cccccgcaac ctcggggagg tcctgaagcg ctaccgcaag gtcgtgatcc
     3901 ccgagatcaa cctggggcag ctgcgcaagc tcatccgcgc cgagttcctg gtggacgcga
     3961 tcgggtacaa ccgcgtccgc gggctcccgt tccgcgcggc cgacctggaa gaagccctgc
     4021 tggaggtcat cgatggctga gtcgggcgag aacgtctccg ggaacggggc cggcgccgcg
     4081 gacgacggac agggcgtcgc cacgctgacg cgcaaagact tccagtcgga ccaggaggtc
     4141 cgctggtgcc cggggtgcgg cgactacgcg gtgctctcgg cggtgcaggg cgtgatgccc
     4201 gagctcggca tcccacgcga gcggatcgtc ttcgtcagcg gcatcggctg ctcgagccgc
     4261 ttcccgtact acctgaacac ctacgggatg cacggtatcc acggccgggc cccggcgatc
     4321 gccacgggca tctccaccag ccgccccgag ctgtcggtgt gggtggcgac cggcgacggc
     4381 gacgggcttt cgatcggcgg gaaccatctg atccacgcga tgcgccgcaa cgtcaacctg
     4441 cgcatcctgc tgttcaacaa cgagatctac ggactgacca aggggcagta ctcgccgacg
     4501 tcggcggccg gcaaggtgac gaagtccact ccgttcgggt cgatcgactg gcccttcaac
     4561 ccggtcagcc tggccctcgg cgccgagtgc acgttcgtcg cccgcgccct ggacgtggac
     4621 aaggcccacc tcaccgagat catgaagcgg gccgccgcgc accgcgggac cgccttcgtc
     4681 gagatctacc agaactgccc ggtgttcaac gacgaccagt tcatcgcgct gaccggcaag
     4741 gaccagaagg ccctgaacca gatccacctt ga
//
LOCUS       DASPWO010000377         6457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1155540, whole genome shotgun sequence.
ACCESSION   DASPWO010000377 DASPWO010000000
VERSION     DASPWO010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155540"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..474
                     /locus_tag="VEO00_08630"
                     /pseudo
     CDS             49..474
                     /locus_tag="VEO00_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
     gene            528..1085
                     /gene="hisH"
                     /locus_tag="VEO00_08635"
     CDS             528..1085
                     /gene="hisH"
                     /locus_tag="VEO00_08635"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HYU58101.1"
                     /translation="MERFGADARVVDSAAGVANADALVVPGVGAFGACMANLETSRLD
                     RAVVAFGASGRPLFGVCLGMQLLHEWSEEGGVDGLGLLPGKVRRLHGPVKVPHMGWNS
                     VAWTRSHPFTAEVAPGGRFYFVHSYACEPDPATTVGEAEHGVRFAAVAARGNLFGTQF
                     HPEKSGPDGLALYARFVEAAREAAR"
     gene            1082..1789
                     /locus_tag="VEO00_08640"
     CDS             1082..1789
                     /locus_tag="VEO00_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HYU58102.1"
                     /translation="MIVFPAIDVLGGRCVRLVRGDPTNRKVYGDPVRIAERLAGEGAT
                     WLHVVDLDAAFGRGSNEQAIRRVIAAAEISVQVAGGIRSTDALRGWLDAGAERVVLGT
                     SAALDQDMLWDLTAAREFDSVVVALDVSGGEVRVKGWTESAGPLAKVAPRMVQAGVQR
                     FQVTAIDRDGTMEGPDLHLFTQVMDLTGVPVIAAGGVATVQDVRDLAKTGVEGVIVGR
                     ALLDGVFTLAQALDAAS"
     gene            1786..2544
                     /gene="hisF"
                     /locus_tag="VEO00_08645"
     CDS             1786..2544
                     /gene="hisF"
                     /locus_tag="VEO00_08645"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HYU58103.1"
                     /translation="MTLAARVIPCLDVDGGRVVKGTRFVDLRDAGDPVELAARYDREG
                     ADELVFLDIAATVEGRASTLGVIAHTAEQVFIPLTVGGGVRSSEDVRTLLRAGADKVA
                     LNTAAVRDPSLLARCADRFGTQCVVVAIDARRRNGSWEVVIVAGRTPTGLDAVAWAAE
                     AAELGAGEVLLTSMDRDGTGEGYDLELLRAVSETVPVPVVASGGAGAVEHFADAILEG
                     KADAVLAASRFHFEQIPIAAVKDHLASRGIPVRR"
     gene            2541..2909
                     /locus_tag="VEO00_08650"
     CDS             2541..2909
                     /locus_tag="VEO00_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58104.1"
                     /translation="MIVVNRSAVWRQPQHAIPAAMYGLVFGFVIFVALGGRPAGWDLL
                     AAVAVGGAVGGFRLWAGTGSRFALISYLGALAIAIPLSVWAMTHSILAGALVLLFAVL
                     VFPAALAGRGGGRRAKDGRR"
     gene            2906..3207
                     /gene="hisI"
                     /locus_tag="VEO00_08655"
                     /pseudo
     CDS             2906..3207
                     /gene="hisI"
                     /locus_tag="VEO00_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006720181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
     gene            3198..4121
                     /gene="hisG"
                     /locus_tag="VEO00_08660"
     CDS             3198..4121
                     /gene="hisG"
                     /locus_tag="VEO00_08660"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HYU58105.1"
                     /translation="MLLAVVRRRRRGEWLDAEARAPEGSLEQQTLALFEAADLAVRRG
                     SSRDYHGRIDDDRIERVSLLRPQEIPRYVEDGFFDLGITGHDWVEETGARIETLASLA
                     YAKGGSGQGVKIVLAVPATSPAERGADLPAGARISTEYPELTKRYFDGLGIPVKVFES
                     YGATEAKVPDIVDAIVDVSETGGTLRAHGMKIVETLLESDVLLVANRDAAADPEKRRA
                     MDELRTLLLGALDAQGPVLIKLNVSEERLKEVLEFLPSMKAPTVSPLAEEGNYAIKTV
                     VEKRAVNRLIPQLKAHGATDILELPISKIVP"
     gene            4127..4498
                     /locus_tag="VEO00_08665"
     CDS             4127..4498
                     /locus_tag="VEO00_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HYU58106.1"
                     /translation="MVTARRFRAVALSLPEAEERETWGEATFRVRDRIFAIMSPDGKR
                     ASIKASPQEQAALIHADPRTYSPAPYVGKHGWVAVELRTADGDEVGELLTEAWRLTAP
                     KRLVAALDGAAQSPSDSQTRR"
     gene            complement(4465..4743)
                     /locus_tag="VEO00_08670"
     CDS             complement(4465..4743)
                     /locus_tag="VEO00_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58107.1"
                     /translation="MVDPVARRGYRFRRTAGVVDGGPLFDRVAAFYRDRGVAPTDEGG
                     RRIKAAVLIRVEAVEPLISPGYDDGTEENAMRGRWFEIYRRVWLSEGD"
     gene            complement(4952..5356)
                     /locus_tag="VEO00_08675"
     CDS             complement(4952..5356)
                     /locus_tag="VEO00_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HYU58108.1"
                     /translation="MRTETLEIDTRGRVVTDVTDRVERFAAAGGESGLLHVFLPHATA
                     GLAIIETGAGSEGDLQDAIERLLPRDDRYTHRHGSPGHGADHLLPALVPPSLVLPVED
                     GRPVLGTWQRVVIVDPNRENNRRTLRLSLLGG"
     gene            complement(5555..6223)
                     /locus_tag="VEO00_08680"
     CDS             complement(5555..6223)
                     /locus_tag="VEO00_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HYU58109.1"
                     /translation="MADPIQAVPLARPDGRIRMLARLSARDAAAWRRAVRGLVPALER
                     VLGPEVLANRTGPGCSGGWRGDLRRARRLAAALRRSAPLLLRTDVRAFYPSVTPSALS
                     RALTAAGVPREPAREAMHMLEAWSAGGHAGLPVGPPPSAVLANAVLAPADRALRAAGL
                     PFLRWVDDYLIALPWGRPEAALEPLDEKLAAWGLLRAPEKTALVDPAEPLRWLGGASR
                     SRNR"
BASE COUNT          845 a         2257 c         2489 g          866 t
ORIGIN      
        1 ggggtgcgcg aggtctaccg cctcggcgga gcccaggcga tcgcggcgat ggcgtacggg
       61 accgagacca tccggccggt cgagcgaatc gtcggcccgg gcaacgcctg ggtcaccgcg
      121 gcgaaggccg aggtggcggg cgtcgtcggg atcgacgggc tgaccggtcc gaccgagctg
      181 gtggtcgtgg ccgacggatc ggcggacccg accgtcctcg ccgccgacct gatcgcccag
      241 gccgagcacg cgcccgacgc cgaggcgtac gtcgtgacct gggacgagcc gctcgtgccc
      301 gcggtgcgtg cggcgctgcg ggtcgaggcc gagaccgccc accgacggga gatcctggat
      361 cgggcgctca ccctcgcccg ggcgctcctg gtgcgtgacg ccgcccaggc ggctaccgtg
      421 gcagaccgga tcgcgcccga gcacttgcag gtggtggtcg ccggcccccg gggcttcctg
      481 gagcggtgcc gtcgctacgg ggccgccttc ctcggcccca gcacgccgtg gagcggttcg
      541 gcgccgacgc gcgggtcgtc gactcggcgg cgggtgtggc gaacgccgac gcgctcgtgg
      601 tgccgggcgt cggcgcgttc ggcgcgtgca tggcgaacct ggagacgagc cgcctggacc
      661 gggccgtcgt cgcgttcggg gcgagcggac ggccgctgtt cggcgtctgc ctcgggatgc
      721 agctgctgca cgagtggagc gaggaaggcg gcgtggacgg cctcggcctg cttccgggca
      781 aggttcgccg gctccatggc ccggtgaagg ttccgcacat ggggtggaac tccgtagcct
      841 ggacgcgctc gcacccgttc accgccgagg tggcgcccgg cgggcgcttc tacttcgtcc
      901 actcctacgc ctgcgagccg gacccggcga cgacggttgg cgaagccgag cacggggtgc
      961 ggtttgcggc cgtcgcggcg cgcggcaacc tgttcgggac ccagttccac ccggagaagt
     1021 ctggtcccga cggcctggcg ctctacgcgc ggttcgtcga ggcggcgcgg gaggcggcgc
     1081 gatgatcgtc ttcccggcga tcgacgtcct cggcggccgc tgcgtgcgcc tggtgcgcgg
     1141 cgatccgacc aaccggaagg tctacggcga cccggtgagg atcgctgaac gtctggccgg
     1201 cgagggcgcg acgtggctcc acgtggtgga cctcgacgcg gccttcggga ggggctcgaa
     1261 cgagcaggcc atccggcggg tgatcgccgc ggccgagatc tcggtgcagg tggccggcgg
     1321 gatccggagc accgacgcgc tgcggggctg gctcgacgcg ggcgcggagc gggtggtgct
     1381 cgggacgagc gccgcgctcg accaggacat gctgtgggac ctgaccgccg ccagggagtt
     1441 cgactcggtc gtcgtcgccc tcgacgtctc cgggggggag gtacgggtga aggggtggac
     1501 cgagtcggca ggcccgctgg cgaaggtggc gccgcgaatg gtccaggccg gcgtgcagcg
     1561 gttccaggtc accgccatcg accgtgacgg gacgatggag gggcccgacc tgcacctgtt
     1621 cacgcaggtg atggacctga ccggggtgcc cgtgatcgcc gcgggcgggg tggccaccgt
     1681 gcaggacgtc cgcgacctgg ccaagaccgg tgtcgagggc gtgatcgtcg gccgggcatt
     1741 gctcgacggc gtcttcaccc tggcgcaggc tctggatgcg gcctcatgac gctcgcggcg
     1801 cgcgtcatcc cctgcctcga cgtcgacggc gggcgcgtgg tgaaggggac gcggttcgtg
     1861 gacctgcgcg atgcgggcga tccggtggag ctcgccgcgc gctatgaccg cgagggggcc
     1921 gacgagctgg tgttcctgga catcgccgcg accgtcgagg gacgcgcgtc gacgctcggc
     1981 gtgatcgcgc acaccgccga gcaggtgttc atcccgctga ccgtcggcgg gggcgtccga
     2041 agctcggagg acgtccgcac gctgttgcgc gccggcgccg acaaggtcgc gctgaacacc
     2101 gccgcggtgc gcgacccgtc gctcctggcc cgctgcgccg accgcttcgg cacgcagtgc
     2161 gtcgtggtgg cgatcgatgc gcggaggagg aacggctcct gggaggttgt gatagtggcg
     2221 gggcggacgc caaccgggct ggacgcggtg gcctgggcgg ccgaggcggc ggagctcggc
     2281 gccggcgagg tgctgctgac ctcgatggat cgcgacggga ccggcgaggg ctacgacctc
     2341 gagcttctgc gcgccgtctc cgagacggtg ccggtcccgg tggtcgcctc cggcggcgcc
     2401 ggcgcggtgg agcacttcgc cgacgcgatc ctcgagggga aggccgacgc ggtgctcgcc
     2461 gccagccgct tccacttcga gcagatcccg atcgccgcgg tgaaggacca tctggcctcc
     2521 cgcggcatcc cggtcaggcg atgatcgtgg tgaaccgttc cgcggtgtgg cgacaaccgc
     2581 aacacgctat tcctgcggcc atgtacggcc tcgtcttcgg gttcgtgatc ttcgtcgcgc
     2641 tcggcggacg tcctgcgggc tgggacctgc tcgcggccgt cgctgtcggt ggggcagtcg
     2701 gtgggttccg cttgtgggcg ggcaccggct cgagattcgc gctgatctcc tatctcgggg
     2761 cgctggcgat tgcgatccct ctctcggtct gggcaatgac tcactccatc cttgccgggg
     2821 cgcttgtgct cctgttcgcg gtgctcgtat tccccgccgc ccttgcggga agaggagggg
     2881 gccgtcgggc gaaggacggg cgtcggtgaa caccggcgac ctgcggttcg acgatcgggg
     2941 gctgatcccg gcggtggtgc aggacgaggg gacccgagag gtgctgatgg tggcgtggat
     3001 gaacgaggac gccgtgcggc ggacgctgca gtccgggccg acctggttct tcagccggtc
     3061 gcggcaggag ctgtggcgca aggcgacacc agcgggaacg cgcagcacgt gcgcgacgtg
     3121 cgctacgact gtgacggcga caccctgctc gtgctcgtcc atcaggaggg gcccgcctgc
     3181 cataccggca accggacgtg cttctagcgg tcgttcggcg acgccgtcgg ggagaatggc
     3241 tagatgctga agctcgtgct cccgaagggt ccctcgagca gcagaccctg gcgctgttcg
     3301 aagcggccga cctggccgtc cgtcgcgggt cgagccgcga ctaccacggc cggatcgacg
     3361 acgaccgcat cgagcgcgtc tcgctcctcc gcccgcagga gatcccacgc tacgtcgagg
     3421 acgggttctt cgacctcggc atcaccggcc acgactgggt cgaggagacg ggtgcgcgga
     3481 tcgagacgct ggcgtcgctc gcctacgcca agggcgggag cggccagggc gtgaagatcg
     3541 tgctggcggt gccggcgacc tcgccggccg agcgcggggc cgacctcccc gccggcgccc
     3601 gtatctcgac cgagtacccg gagctgacga agcgctactt cgacggcctg ggcatcccgg
     3661 tgaaggtgtt cgaatcctac ggggccaccg aggcgaaggt gcccgacatc gtcgacgcga
     3721 tcgtggacgt cagcgagacc ggcgggacgc tccgcgcgca cgggatgaag atcgtcgaga
     3781 cgctcctcga gtccgacgtg ctgctggtgg cgaaccgcga tgccgccgcg gacccggaga
     3841 agcgccgggc gatggacgag ctgcgcacgc tgctgctcgg cgcgctcgac gcgcaaggcc
     3901 cggtgctgat caagctgaac gtgtcggagg agcgcctgaa ggaggtgctc gagttccttc
     3961 cctcgatgaa ggcgccgacg gtcagcccgc tggcggagga ggggaactac gcgatcaaga
     4021 cggtggtcga gaagcgggcc gtgaaccggc tgatcccgca gctgaaggca cacggcgcga
     4081 ccgacatcct cgagctcccg atctcgaaga tcgtcccctg acgagcgtgg tcacggccag
     4141 gcggttccgc gcggtggcgc tgtcgttgcc ggaggcggag gagcgcgaga cctggggcga
     4201 ggccacgttc cgggtccgcg accggatatt cgccatcatg tccccggacg ggaagcgggc
     4261 ctcgatcaag gcgtcgcccc aggagcaggc cgcgctgatc cacgccgacc cgcggaccta
     4321 ctcgccggcg ccctacgtcg ggaagcacgg ctgggtggcg gtcgagctcc gcacggccga
     4381 cggggacgag gtcggggagc tgctgaccga ggcctggcgc ctgaccgccc cgaagcgcct
     4441 cgtggccgcc ctcgatggcg ccgctcagtc gccctcggac agccagaccc ggcggtagat
     4501 ctcgaaccac cggccgcgca tcgcgttctc ctcggtgccg tcgtcgtagc ccggcgagat
     4561 cagcggctcg acggcctcga cccggatgag gaccgccgcc ttgatccggc gccctccctc
     4621 gtccgtcggt gccacgcccc ggtcgcggta gaaggccgcc acgcgatcga agagcggacc
     4681 gccgtccacc acgcccgccg tccggcggaa gcggtagccc ctgcgcgcca ccgggtcgac
     4741 cacgttgaac ctccgccgcc gggcgctcgc gggcgttggc gaccgtgcgc ggcgaagcga
     4801 tgtgggcgaa caccagatga tcgtcgtccc agacggtcgt cgtgcccttc ggcgacaggt
     4861 tcggggttcc gtcggcgttc acggtcgcca tgtacccgag ccgctgcgcc cggacgaacg
     4921 cctgcacctc cggcgagagc cgccccactc gtcagccccc gagcagggag aggcggaggg
     4981 tccggcggtt gttctcgcgg ttcgggtcga cgatcaccac acgctgccac gtgccgagca
     5041 ccgggcggcc gtcctcgacc ggcagcacca gcgagggcgg gacgagcgcc ggcagcaggt
     5101 ggtcggcgcc gtggccgggc gagccgtggc ggtgcgtgta gcgatcgtct cgcggcagca
     5161 ggcgctcgat cgcgtcctgc aggtccccct ccgagccggc gccggtctcg atgatcgcga
     5221 gcccggccgt ggcgtgcggc aggaagacgt ggagcagccc gctctccccg cccgccgcgg
     5281 cgaagcgttc cacccggtcg gtcacgtccg tgaccacccg cccgcgcgtg tcgatctcga
     5341 gggtctcggt ccgcacgctg cacggcagcg tacccgcctc ggcaggacgg gtcgaggatc
     5401 gagcgtgacc cgaggacctg cgggaacgcg tcgcgcgggc ccgcgggggc ccgcgcgtgc
     5461 gtgagagcgc ccgacggcgc gaccggaacc ggcccgaggg ccggcccgga gaggcggcgc
     5521 gtgcactgat catcgacgtc cccgagccgt tcctttaccg gttccgggaa cgggaagccc
     5581 cgcccagcca ccggagcggc tccgccggat ccaccagcgc ggtcttctcc ggcgcgcgga
     5641 gcaggcccca cgccgccagc ttctcgtcga gcggctccag cgccgcctcg ggccggcccc
     5701 agggaagcgc gatgaggtag tcgtcgaccc agcgcaggaa cggcagcccg gccgcccgca
     5761 gcgcccggtc ggcgggagcg agcaccgcgt tcgccaggac ggccgagggc ggcggcccga
     5821 ccggcaggcc ggcgtggccg cccgccgacc aggcctcgag catgtgcatc gcctcgcggg
     5881 ccggctcgcg gggcacaccg gccgcggtga gcgcgcggga aagcgcggac ggggtcaccg
     5941 acgggtagaa cgcccggacg tcggtccgca gcaggagcgg ggcggaacgt cgcagggccg
     6001 cggccaggcg ccgggcgcgg cgcaggtcgc cccgccaccc gccggagcac cccgggccgg
     6061 tccggttcgc cagcacctcg ggcccgagga cgcgctcgag ggccggcacg agcccccgga
     6121 cggcacgccg ccaggccgcg gcgtcccgcg ccgacaggcg cgcgagcatc cgtatccgcc
     6181 cgtccgggcg ggccagcggc acggcctgga tcggatccgc cacgtctccc ccagcgggcc
     6241 ggaggaggcg cggccgggtg cggttgtacc ggagcgcgcg gctcgggaca tccccccggc
     6301 gggtggttct gtacctccgg gcgggggtct gctaggcccc caggcgggcc agcagcagcg
     6361 cctcggccag gcaggtccgg tggagctcgc cgaggtgcac gctctcgttc gccccatggg
     6421 ctcgtccatc gggatcctcg acgccggtga ccaggat
//
LOCUS       DASPWO010000378         5228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_566604, whole genome shotgun sequence.
ACCESSION   DASPWO010000378 DASPWO010000000
VERSION     DASPWO010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566604"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VEO00_08685"
     CDS             <1..318
                     /locus_tag="VEO00_08685"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674458.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HYU58110.1"
                     /translation="LADEDGQVLDAESISAGLDYPGSGPEHAFLRDSGRAEYATATDD
                     EALAAFRRLASTEGILPALEPAHVIGWLLRRPLPVGSGVLVCLSGRGDKDLDTVRAAL
                     SRE"
     gene            315..1091
                     /gene="trpA"
                     /locus_tag="VEO00_08690"
     CDS             315..1091
                     /gene="trpA"
                     /locus_tag="VEO00_08690"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HYU58111.1"
                     /translation="MTGLEGALRGRLAVGGKAFLPYVTGGLPEVDVTLLHELAEAGAD
                     AIEVGIPFSDPVMDGPVIQDASRLALEAGATLEGVLDLVAAAAPATPVAVMTYLNPVL
                     AIGEDAFLERAVAAGVAGVIVPDLPVDEGEEWTARCRARDVASVYLAAPGTGPDRLAA
                     IGKASSGFVYCVSTYGVTGRRAALSETSRQVVEALRRLTDLPLLVGVGISTPEQAAEA
                     CSFADGVVVGSALMARLLDDDHDGALSLIRDFRAAVRESS"
     gene            1132..1797
                     /locus_tag="VEO00_08695"
     CDS             1132..1797
                     /locus_tag="VEO00_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYU58112.1"
                     /translation="MRSREERLEVLGLIEAGWNNSQIARQTGISRPTIRDWRMHGIVP
                     RTRPSTCPRCDGGSMSEHAYAYLLGLYLASNHRRGETIHHRSPSRTAAASVPRSWGRS
                     PGLDLLLEELAVPLPTAWPGRKHLRPIWLADWQEEVVRRYPQQFLKGLIESDGCRVLN
                     RVAGHAYPRYQFSNVSADIRDIFCWACDLLGVEWKQSRWNTISVSKRRYVHYLDTFIG
                     PKG"
     gene            1824..1908
                     /locus_tag="VEO00_08700"
     tRNA            1824..1908
                     /locus_tag="VEO00_08700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1859..1861,aa:Leu,seq:caa)
     gene            1960..2211
                     /locus_tag="VEO00_08705"
     CDS             1960..2211
                     /locus_tag="VEO00_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58113.1"
                     /translation="MKARPLVVVGIVLQAAVGALVLVSGLIMPIWAVIALGVVWAIGT
                     GLAIRWRRRPSLVLLVPLVVLAIWALAAWAGEAFLDWTA"
     gene            complement(2221..2502)
                     /locus_tag="VEO00_08710"
     CDS             complement(2221..2502)
                     /locus_tag="VEO00_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HYU58114.1"
                     /translation="MSGTEALITVRVRPRSRPGLTLLGDAIVVRVAAAPAGGAATDEA
                     RRALARAFGVAPSAVTLRMGTRRREKTFAVAGLTREEATVRLRRAAGRP"
     gene            2594..2908
                     /locus_tag="VEO00_08715"
     CDS             2594..2908
                     /locus_tag="VEO00_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58115.1"
                     /translation="MTRRLQVKSRLVAVSEEMDRARSEIEILREQLRFQEDVLEETRV
                     RLLVAETPLADREFRIASDDHRRIERVLQDAEGTLAALAEEQDRLLAALSETALPERE
                     AG"
     gene            2916..3512
                     /locus_tag="VEO00_08720"
     CDS             2916..3512
                     /locus_tag="VEO00_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018349869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYU58116.1"
                     /translation="MSQEARAPTRVLIAEDEALIRMDLREMLEEEGYEVVGEAGDGET
                     AVRLARDLRPDLTVMDIKMPGLDGLSAAERITEERLSPVLILTAFSQKDLVERAAQTG
                     AMGYLVKPFQKSDLVPAIDIAVVRYQELLALEGEVSVLEERLETRKLVDRAKGLLMDN
                     YAMSEKDAFRFIQKSAMDRRLPMAEVARKVVEGELRAD"
     gene            3621..4844
                     /locus_tag="VEO00_08725"
     CDS             3621..4844
                     /locus_tag="VEO00_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HYU58117.1"
                     /translation="MRKPRWVFLVALLGVFALVAAACNNDGGGGGGATTGGAERIDVT
                     VYGVGAWTGPYNYLVIPSFQAAQLRFDELNAEDGYPATITLAQGDTQGSGDNAPPVVE
                     EVVNDPNTVAVLGPAFSGESEAAGDTFDENGIPFVTQSATNPGLAEKGWTFWYRGCGN
                     DTSQGTLAAQYVVQELAPTALFVSHDKSTYGQGLAEVVRDTAIDSDVNVIEFAGVEAG
                     QDDYSNFISDVQASGADVLFYGGYDADFGKIVAQGRDAGLDIPMMSGDGSLSSTFLDL
                     AGDAAEGVTLIAPTNLSGDLVDRYNTELGSEASSVPIYVPEGYDVASLIGEGIRAAIE
                     GGAEAPEDIRTGIKEYLDSLTSTNPFVGEAKPYSFDPSTHEINSAQPDDLFFIYQVGP
                     DGVTQLGSASEVLGG"
     gene            4939..>5228
                     /locus_tag="VEO00_08730"
     CDS             4939..>5228
                     /locus_tag="VEO00_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017988042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYU58118.1"
                     /translation="MECVTNVAHQFWAQTVNGLSAGSIYAMIALGYTMVYGVLQLINF
                     AHSEVFMVSVVVTLYSYRNWWSLDAPQSGIVLVLILLTTVLAAMAVSGLTAVT"
BASE COUNT          777 a         1776 c         1872 g          803 t
ORIGIN      
        1 ctggccgacg aggacgggca ggtgctcgac gccgaatcga tctccgccgg tctcgactat
       61 ccgggctccg ggcccgagca cgcatttctg cgcgacagcg gccgcgccga gtacgccacg
      121 gcgaccgacg acgaagccct cgccgctttc aggcgcctcg ccagcaccga ggggatcctg
      181 ccggccctcg agccggccca cgtgatcggc tggctgctgc gccgcccgct ccccgtgggc
      241 tcgggcgtgc tggtgtgtct ctccggccgc ggggacaagg acctggacac cgtccgcgcc
      301 gcgttgtctc gcgaatgacc ggcctcgagg gcgccctccg cggccgcctg gctgtggggg
      361 ggaaggcctt cctcccgtac gtcaccggcg gcctcccgga ggtcgatgtc acgcttctgc
      421 acgagctggc ggaggccggc gccgacgcga tcgaggtggg catcccgttc tccgacccgg
      481 tgatggacgg gccggtgatc caggatgcct cccggctggc cctggaggcc ggcgccacgc
      541 tggagggggt cctcgacctg gtcgccgccg cggcgccggc gacgccggtg gcggtgatga
      601 cctacctcaa cccggtcctg gcgatcggtg aggacgcgtt tctggaacgg gccgtggccg
      661 ccggcgtggc cggcgtgatc gtgcccgacc tcccggtgga cgaaggcgag gagtggaccg
      721 cccgctgccg tgcgcgggat gtcgccagcg tgtatctggc cgcgccgggc accgggcccg
      781 atcgcctggc cgcgatcggg aaggcatcga gcggcttcgt gtactgcgtc tcgacctacg
      841 gcgtcaccgg ccgccgcgcc gcgctgtcgg aaacctcgcg ccaggtggtc gaggccctcc
      901 gccggctcac cgacctgccg ctcctggtcg gagtcgggat ctccacgccc gagcaggccg
      961 ccgaagcctg ctcgttcgcg gacggcgtgg tggtcggcag cgccctgatg gcccgcctcc
     1021 tcgacgacga ccacgacggc gcgctttccc tgatccgcga cttccgcgcg gcggtgcggg
     1081 aatcctcgta acggcgttcc ttgtcagacc ctcccgtcac gatcaaccag catgcgctca
     1141 cgcgaggaac gcctcgaagt cctcggtctg atcgaggccg gctggaacaa ctcacagatc
     1201 gcccggcaga ccggcatatc gcggcccacg attcgtgact ggcggatgca cggcatcgtg
     1261 ccgcgaacgc gcccgtcgac atgcccgcgt tgtgacggtg gttcgatgag cgagcacgcc
     1321 tatgcgtacc tgctcgggct ctacctggct tcgaatcatc gacgagggga gacgatccat
     1381 caccggtctc cttcccgaac ggcggctgcg agtgtgcctc gttcgtgggg taggagtcca
     1441 ggtcttgacc tcctcttgga agaactggcc gtgcctcttc ccacagcatg gcccggccga
     1501 aagcacctcc gaccgatctg gctcgctgac tggcaggagg aggtcgtccg tcgatacccg
     1561 cagcagttcc tgaagggctt gatcgaatcg gacggatgtc gcgtgctgaa ccgagtggcg
     1621 gggcatgctt atccccggta tcagttttcc aacgtgtccg cggatattcg ggacatcttc
     1681 tgttgggcgt gcgatctcct tggagtggag tggaagcagt cgaggtggaa cacgatctcg
     1741 gtgagcaaac ggcggtatgt gcattacctc gacacgttca tcggcccgaa ggggtgaagg
     1801 gggtgcggct acaatcgatg tgagccgggg tagccgaatt cggtacaggc gatggtctca
     1861 aacaccattg ggcgaaagcc cgtgcgggtt cgaatcccgc ccccggcacg gcgttagggt
     1921 acggctcacg atctggcccg ccggagggag gctccatcga tgaaggctcg gccgttggtc
     1981 gtcgtgggga tcgtcctgca ggctgccgtc ggggcgctcg tgctggtgag cggcctgatc
     2041 atgccgatct gggccgtcat cgccctcggc gtcgtgtggg ccatcgggac gggcctggcg
     2101 atccggtggc gcaggcggcc cagcctcgtc ctcctggtcc cgctcgtggt gctcgcgatc
     2161 tgggccctcg cggcctgggc cggcgaggcg ttcctggact ggacggcctg aggaaccgga
     2221 tcaagggcgc ccggcggcac ggcgcagcct gaccgtcgcc tcttcgcggg tcaggccggc
     2281 cacggcgaag gtcttctccc gtctgcgcgt tcccatgcgc agcgtgaccg cggagggcgc
     2341 gacgccgaag gcgcgggcga gggcgcggcg cgcctcgtcg gtggccgcgc cgcccgccgg
     2401 ggcggccgcc acccggacca cgatcgcgtc tccgagcagg gtgagccccg ggcgcgagcg
     2461 cggccggacc cgcaccgtga tcagcgcctc cgtcccgctc acggccgcat catgcctcgc
     2521 ttgcgggtgc gcggcgccgt acctagactt tacgccccca ctcgcaccga ccactccagc
     2581 gggagcactc cccatgaccc gtcgactgca ggtcaagagc cgcctcgtcg ccgtctccga
     2641 ggagatggac cgggcgcggt ccgagatcga gatcctgcgc gagcagctgc gcttccagga
     2701 ggacgtcctg gaggagacgc gcgtccgcct gctggtggcg gagacgcccc tcgccgaccg
     2761 cgagttccgg atcgcctccg acgaccaccg ccgcatcgag cgcgtcctcc aggacgccga
     2821 agggaccctc gcggccctgg ccgaggagca ggaccggctg ctggcggcgc tctccgagac
     2881 tgcccttccg gagcgcgagg cgggatgacc ccgggatgag ccaggaggcg cgcgcgccga
     2941 cccgggtgct gatcgccgag gacgaggccc tgatccggat ggacctgcgc gagatgctcg
     3001 aggaggaggg ctacgaggtg gtcggggagg ccggcgacgg cgagaccgcc gtccgactgg
     3061 cccgcgacct tcgtcccgac ctgaccgtga tggacatcaa gatgcccggc ctcgacggcc
     3121 tctccgctgc cgagcggatc accgaggagc gactctcgcc cgtgctgatc ctgaccgcct
     3181 tcagccagaa ggacctggtg gagcgggcgg cccaaaccgg cgccatgggc tacctcgtaa
     3241 aaccctttca gaagagcgac ctcgttccgg ccatcgacat cgcggtggtc cggtaccagg
     3301 agctcctcgc gctggaagga gaggtctccg tgctggagga gcgcctggag acgcggaagc
     3361 tggtggaccg ggcgaagggg ctgctgatgg acaactacgc gatgtccgag aaggacgcgt
     3421 tccggttcat ccagaagagc gccatggacc gccggctgcc gatggccgag gtcgcccgca
     3481 aggtggtcga gggcgagctc cgggccgact gaggaggggc ccgaacgggt atgccaggtc
     3541 agatggccac ggaccgccga ttgtggtacc tacagcacgg aagcagagcg tgcgcgacgc
     3601 gcgcgcggaa ggagggggaa atgaggaagc cacggtgggt cttcctggtc gcgctgctcg
     3661 gtgtgttcgc gctggtggcc gcggcctgca acaacgacgg gggcggcgga ggcggcgcca
     3721 ccaccggcgg cgctgagcgc atcgacgtga ccgtctacgg cgtgggcgcc tggaccgggc
     3781 cgtacaacta cctggtcatt ccgagcttcc aggccgccca gctccgcttc gacgagctga
     3841 acgccgagga cgggtacccc gccaccatca ccctggcgca gggcgacacg caggggtccg
     3901 gtgataacgc cccgcccgtg gtcgaggagg tcgtcaacga cccgaacacc gtcgcggtgt
     3961 tgggcccggc gttctcgggc gagtccgagg cggcgggcga cacgttcgac gagaacggga
     4021 tcccgttcgt cacgcagtcg gccacgaacc cgggcctggc ggagaagggc tggacgttct
     4081 ggtaccgcgg ctgcgggaac gacacctcac agggcacctt ggcggcgcag tacgtcgtcc
     4141 aggagcttgc cccgaccgcg ctgttcgtgt cgcacgacaa gtccacgtac ggccaggggc
     4201 tggccgaggt ggttcgcgac acggcgatcg acagcgacgt caacgtcatc gagttcgccg
     4261 gcgtcgaggc ggggcaggac gactactcga acttcatcag cgacgtccag gcctcggggg
     4321 cggacgtgct gttctacggc ggttacgacg cggacttcgg caagatcgtg gcgcagggtc
     4381 gggacgccgg gctggacatc ccgatgatgt ccggcgacgg ctcgctgtcg tcgacgttcc
     4441 tcgacctcgc cggtgacgct gccgagggcg tgacgctgat cgcgccgacc aacctctccg
     4501 gcgacctcgt cgatcggtac aacacggagc tggggagcga ggcctcgtcg gtgccgatct
     4561 acgtcccgga gggctacgac gtcgccagcc tgatcggcga gggcatccgc gcggcgatcg
     4621 agggcggcgc cgaggcgccg gaggacatcc ggaccgggat caaggagtac ctggacagcc
     4681 tgacctcgac caacccgttc gtcggggagg ccaagccgta ctccttcgac ccctccactc
     4741 acgagatcaa ctcggcccag cccgacgacc tcttcttcat ctaccaggtc gggcccgatg
     4801 gggtgacgca gctgggcagc gcgtctgagg tgctgggcgg ctaggcgagg gaaggaccgc
     4861 agcggaacgc gggagggcag gcgcgccacg cctgccctcc cggcatgctg cgaacgctcg
     4921 gcgtgggggt ggacctgagt ggagtgcgtc accaacgtcg cccaccagtt ctgggcccag
     4981 accgtcaacg gcctgtccgc gggctcgatc tacgcgatga tcgccctcgg ctacacgatg
     5041 gtctacggcg tgctgcagct gatcaacttc gcgcactccg aggtcttcat ggtgtcggtg
     5101 gtcgtcacgc tgtactccta ccggaactgg tggtcgctcg acgcgccgca gagcgggatc
     5161 gtcctggtgc tgatcctctt gaccacggtc ctcgcggcga tggccgtctc ggggctgacg
     5221 gcggtcac
//
LOCUS       DASPWO010000379         3547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_476551, whole genome shotgun sequence.
ACCESSION   DASPWO010000379 DASPWO010000000
VERSION     DASPWO010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476551"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VEO00_08735"
     CDS             <1..325
                     /locus_tag="VEO00_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011896245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYU58119.1"
                     /translation="SRCRTCPVRERCTRSKKGRQMQIHQHHHLLMAARRQAKTEEFQT
                     IYRQHRPMVERSIAWVVARGHRRVRYRGVARNQLGLSLRVAAINLRRLINLGLDHQGG
                     WVLAT"
     gene            637..1257
                     /locus_tag="VEO00_08740"
     CDS             637..1257
                     /locus_tag="VEO00_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYU58120.1"
                     /translation="MIDVSVIDDPEAAEVSLDPIRTRLLAQLAEPASATAVAARVGLP
                     RQKVNYHLRTLEQHGLIELVEERRKGNMTERVLRATALSYVISPAALAAVEPDPSRAP
                     DRLSARWLIALAARLVRDVGQLLAGATKARKRLATFAMDGEIHFASAADRAAFAEELA
                     RAVTGLIGKYHDEHAPGGREHRVLVAVHPSLKPEPPAERPIEREGP"
     gene            1257..2006
                     /locus_tag="VEO00_08745"
     CDS             1257..2006
                     /locus_tag="VEO00_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYU58121.1"
                     /translation="MAHPFELTDQFQVDATPEEVWEAIATGPGVDGWFMGRNEIEPRE
                     GGTIRTAMRGSVEEATVTAWEPPTRFASRTPEGPDGAFHAFEYIVEGSEKGSTVVRWV
                     HSGFLGENWETEYEGLSEGDPMYFDKLRVYLTYFRGRRATPVEVFGPQVPDRDKAWEL
                     LYGGLGLAGAPSLGDSVRLTPEGLPELVGVVDWLSRDFLGVRTDDGIYRFMHISAFGG
                     PTGVGHHIFVDGLDQAEAERAWSSWLNAVFS"
     gene            2030..2806
                     /locus_tag="VEO00_08750"
     CDS             2030..2806
                     /locus_tag="VEO00_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYU58122.1"
                     /translation="METVTSRDGTTIAFDRLGQGPPVVLVSGGSVDRTSNAALAQELA
                     SDFTVLNYDRRGRGPSGDIPPYAIEREIEDIEAVIEAVGGEASLYGSSSGAVLAMFAS
                     EAGLPVAKLAMWEPPFIPEGFPTPPEDQVEQYERMVADGRRGDAVEYFMSKVVGLPPE
                     FVEGAKQQPFWAATEALAHTLAYDARIMSDYRIPVARATGVKVPALVIAGGADMPWMR
                     DTAAALAEALPQGETRFLDGQGHDVDPTVLAPVLREFFAA"
     gene            2803..3225
                     /locus_tag="VEO00_08755"
     CDS             2803..3225
                     /locus_tag="VEO00_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU58123.1"
                     /translation="MKPRITILTIGVDDLEASVRFYRDGLGLPTEGIVGTEFEHGAVA
                     FFELQPGMQLAAWPRGSIAHDTGIPQAPRSPTEFTIGHNVASRDAVDEVMRQAAVAGA
                     TIVKPAQETFWGGYAGYFQDPDDHLWEVAWNPSIDVED"
BASE COUNT          560 a         1211 c         1286 g          490 t
ORIGIN      
        1 ctcccggtgc cggacctgcc cggtgcgcga gcggtgcact cggtccaaga agggacgcca
       61 gatgcagatc caccaacatc accacctgct catggccgcg aggcgccagg cgaagaccga
      121 agagttccag accatctacc gacaacaccg accgatggtg gagcgctcga tcgcctgggt
      181 cgtggcccgg ggccatcgaa gggttcgtta ccggggcgtc gcccgcaacc agctcggcct
      241 gtcgctcagg gtcgcggcca tcaacctccg ccgcctaatt aacctggggt tagaccacca
      301 aggaggctgg gtcctggcca cctgagaacc ggggaggaaa cccccggccg gatcggtggt
      361 ggttcctgag atcgatctgc cgtggcaatt cgctcgcaga tcgctcccca tcacggacgc
      421 tcccaagaga accagcccga aggggccttg ttcagcaggc tcctagaagg agatccccag
      481 cagcgcggct gcgtacgaaa cactccccac cacggccaca cccaccgggt agtcactgcc
      541 aagcgagatg tcgaggagac gcatcggccg taatccgcgg gccgcaagcc cggggcccgc
      601 ttgacaagaa aagttgtcgg tggataggat cgggtcgtga tcgacgtgag cgtgatcgat
      661 gacccggagg cggccgaggt gtccctcgac ccgatccgga cgcggctcct cgcccagctg
      721 gcggagccgg cgtcggccac cgccgtggcg gcccgggtcg gcctgccccg gcagaaggtc
      781 aactatcacc tccggaccct cgagcagcac gggctgatcg agctggtcga ggagcgccgg
      841 aagggcaaca tgaccgagcg ggtcctccgg gcgaccgcgc tctcctacgt catctcgccc
      901 gccgcgctcg ccgcggtcga gccggacccg tcgcgggcgc cggaccggct gtccgcccgc
      961 tggctgatcg ccctggcggc gcgcctcgtc cgcgacgtcg gtcagctgct cgccggcgcc
     1021 acgaaggcgc ggaagcggct cgccacattc gcgatggatg gcgagatcca tttcgcctcg
     1081 gccgccgatc gcgcggcgtt cgccgaggag cttgcgaggg ccgtcaccgg cctgatcggg
     1141 aagtaccacg atgagcatgc ccccggcggc cgagagcacc gcgtgctcgt ggccgtccac
     1201 cccagcctga agccggagcc gcccgcagag cggccgatcg agagggaggg accctgatgg
     1261 cgcatccgtt cgagctcacc gaccagttcc aggtcgacgc gacccccgag gaggtgtggg
     1321 aggcgatcgc cacggggccg ggcgtcgacg gctggttcat gggacggaac gagatcgagc
     1381 cgcgggaggg cgggacgatc cgcaccgcga tgcgcggctc cgtggaggag gcgacggtga
     1441 cggcctggga gccgccgacg cggttcgcca gccgtacgcc ggagggcccc gacggcgcgt
     1501 tccacgcgtt cgagtacatc gtcgagggca gcgagaaggg aagcacggtc gtccggtggg
     1561 tgcatagcgg cttcctcggc gagaactggg agacggagta cgagggcctg tccgagggcg
     1621 acccgatgta cttcgacaag ctccgcgtgt acctgacgta cttccgcggc cgcagggcca
     1681 cgccggtcga ggtcttcggg ccgcaggtcc ccgatcggga caaggcctgg gagctcctct
     1741 acggtgggct cgggcttgcc ggcgcgccgt cgctcggcga ctcggtccgg ctcacccccg
     1801 agggcctgcc ggagctcgtc ggcgtcgtcg actggctgtc gcgggacttc ttgggcgtgc
     1861 gcaccgacga cgggatctac cgcttcatgc acatttccgc gttcggcggg ccgaccggcg
     1921 tcggccacca catcttcgtc gacggcctcg accaggccga ggcggagcgc gcgtggagct
     1981 cgtggctgaa cgccgtgttc tcatagggtc gaacgaggag gggatgcaga tggagacggt
     2041 cacgtcgagg gacggcacaa cgatcgcgtt cgaccggctc ggacagggac cgcccgtggt
     2101 gctcgtgtcc ggcgggtcgg tggaccggac atcgaacgcg gcgctcgcgc aggagctggc
     2161 ctcggacttc acggtgctca actacgaccg gcgcggccgc gggcccagcg gtgacatacc
     2221 gccgtacgcg atcgagcgcg agatcgagga catcgaggcc gtgatcgagg cggtcggggg
     2281 cgaggccagc ctgtacggct cgtcctccgg tgccgtcctg gcgatgttcg cctcggaagc
     2341 ggggctgcca gtcgcgaagc tcgcgatgtg ggagccgccg ttcatcccgg aggggttccc
     2401 caccccgccg gaggaccaag tcgagcagta cgagcggatg gtcgccgatg gtcggcgcgg
     2461 cgacgcggtc gagtacttca tgtcgaaggt cgtgggcctc ccgcccgagt tcgtcgaggg
     2521 cgcgaagcag cagccgttct gggccgcgac cgaggcgctc gcccacaccc tcgcctacga
     2581 cgccaggatc atgagcgact accgcatccc ggtcgcccgc gcgaccggcg tgaaggtccc
     2641 agcgctggtg atcgccggcg gcgccgacat gccgtggatg cgggacaccg ccgccgccct
     2701 cgccgaggcg ctcccccagg gcgagacgcg cttccttgac gggcaggggc acgacgtcga
     2761 tccgacggtc ctggcgccgg tgctgcggga gttcttcgcc gcatgaagcc gcggatcacg
     2821 atcctcacga tcggcgtgga cgacctcgag gcgtcggtgc gcttctaccg ggacggtctc
     2881 ggactgccga ctgaggggat cgtcgggacc gagttcgagc acggcgccgt ggcgttcttc
     2941 gagctgcagc cggggatgca gctcgcggcg tggccgcggg gctcgatcgc gcacgatacg
     3001 gggatcccgc aggcgccgcg cagcccgacg gagttcacga tcgggcacaa cgtcgccagc
     3061 cgggacgccg tcgacgaggt gatgcggcag gcggcggtgg ccggggcgac gatcgtgaag
     3121 ccggcgcagg agaccttctg gggtggctac gccgggtact tccaggatcc cgacgatcac
     3181 ctctgggagg tcgcgtggaa cccatcgatc gacgttgagg actgaccggc tcgcgggctg
     3241 ggaccctcga cggcgtcccc cctctaagct cgcctgccat gccggacgag cccggaagcg
     3301 tcgatcggga cgtggagcgc ctccgcgtcg cgggcctgct cgcgacgcac gcgccacccg
     3361 attgggagga gcgcttccgc cgcgacgaca agatcgagcg gtagggagcg cggccgaggg
     3421 cccgcaccga tgacatcacg aatgactcac agtcagtgtc gcgggcgcag cccggccagg
     3481 gtgaccgata cgagacgacg gaccgccgcc ttcgaacgct ggtcgccggc cccccctagg
     3541 gcatgca
//
LOCUS       DASPWO010000380         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_136391, whole genome shotgun sequence.
ACCESSION   DASPWO010000380 DASPWO010000000
VERSION     DASPWO010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136391"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1001
                     /locus_tag="VEO00_08760"
     CDS             117..1001
                     /locus_tag="VEO00_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58124.1"
                     /translation="MGTAGAQPTTQSFSFTGGAQSFTVPENVCQITVDAFGAEGGDGG
                     GAVGARGVAGLGGRATGPLGVTPGEALVVTVGGQGVDGDSGAGPAGGFNGGGDGGDGI
                     SIAGGSGGGASDVSRGGTTLIVAGGGGGGGGGSTGSAEGDGGGGGGTGTDGASIPSGA
                     GGGLAGGNGGTGGAASGSGATAGANGGPGTGGDGGDSSPANLQGGGGGGGGATGGGGG
                     GAAGTGGTAFAGAGGGGGSGAGATGTTFEEDVRAGDGEVNITFDPAANTCGGGGAGPG
                     AGAGAPAVAVVAAPQFTG"
     gene            1042..2751
                     /locus_tag="VEO00_08765"
     CDS             1042..2751
                     /locus_tag="VEO00_08765"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HYU58125.1"
                     /translation="MPRLRIALAQLNLVVGDLEGNAARILDAYDRAEADGCDLVAFPE
                     LAISGYPPEDLLLRPAFVAQAQESMEKVAARTGRATAVIGFPLLERDLYNAAAVCANG
                     KVQGVYRKHILPNSTVFDEQRYFAASTTDGPLFNVAGARVAVTVCEDAWSPTGPISTQ
                     AAGGAELVVNINGSPYYAGRLRERETMLSTRAADAPVPIAYVNLVGGQDELVFDGGSL
                     VFDEVGRLVARARQFTEDLLVVDLDVRPTFRKRMLDPRGHRPAPTLPEVKVSDAHLGE
                     RREEPRIEPTLAPVREVYEALVLGTRDYVTKNGFTDAIIALSGGIDSSIVATIATDAL
                     GPERVLCVLMPSRFSSEGSVTDAEAVAANLGVRTITVPIEAAHASFLDMLAEPFAGTE
                     TGLAEENLQARIRGTVIMALSNKFGALVLTTGNKSELATGYSTLYGDMAGGFSVIKDV
                     PKTLVYALARDRNERAGRDLVPAAVLDKPPSAELRPDQRDSDSLPEYSLLDPILEGYV
                     EGDLSVAELEEAGFDPEAVRKVAKLVDHNEYKRRQAPPGVRVSPKAFGKDRRLPITNR
                     WPG"
     gene            2748..>3008
                     /locus_tag="VEO00_08770"
     CDS             2748..>3008
                     /locus_tag="VEO00_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYU58126.1"
                     /translation="MNGRVRAFYPELALVVASFLYGATFTIVQDALDHLTATGFILLR
                     FAIGGAALAPLALRRGWRGPAARPNDSGRVLAGCGVALGFTAF"
BASE COUNT          401 a         1058 c         1118 g          431 t
ORIGIN      
        1 cgtgactact gtgctgcagt tcccgattgc cctggatggt ggcgatgcgg cgttcccggt
       61 tcttggtcgg cgcgagtggc ttcgttctcg cgggagggtt tctcggcctg actggcgtgg
      121 gtacagcagg cgcccagccg acgacgcagt cgttctcgtt caccggcggc gcgcagagtt
      181 tcaccgtccc ggagaacgtg tgccagatca ccgtcgacgc gttcggcgcg gagggcggtg
      241 acggcggcgg ggcggtcgga gctcgcggcg tagcgggact gggtggccgg gcgaccgggc
      301 cccttggcgt cactcccggg gaagcgcttg tcgtgaccgt ggggggccag ggagtcgacg
      361 gcgactccgg cgccggtccc gcgggtggct tcaacggcgg tggtgacggg ggcgacggca
      421 tcagcatcgc cggaggatcg ggcggcggcg cctccgacgt cagccgtggc ggaacgacgc
      481 tgatcgtcgc gggaggcggc ggtggcggcg gcggggggag caccggctca gccgagggtg
      541 atggcggcgg tggcggaggt accggcaccg acggcgcgag catcccgtcg ggtgccggtg
      601 gcggcctcgc cggcggaaac ggcggcacgg gtggcgccgc ttccggctcc ggcgccactg
      661 caggagcaaa cggcgggccc gggaccggtg gcgacggcgg cgactcgagc cccgcgaacc
      721 tccagggcgg cggtggcggc ggcgggggcg cgacgggcgg tggcggcggg ggcgccgcgg
      781 gcacaggggg aacggcgttc gcgggagcag gcggtggcgg agggtccggc gccggggcga
      841 ccggcacaac gttcgaagaa gacgttcgcg cgggcgacgg cgaggtgaac atcacgttcg
      901 accccgccgc gaacacctgc ggcggtggcg gcgccggccc cggcgctggc gctggcgcac
      961 cggcggttgc agtggtggct gcgcctcagt tcacgggctg aggccgctcg tccgaaccgg
     1021 ccgggccgta tccttgaggg cgtgccgcgg ctccggattg ccctcgcgca gctgaacctc
     1081 gttgtcggtg acctcgaggg gaacgcggcg cggatcctcg acgcttacga ccgcgccgaa
     1141 gccgacggct gcgacctcgt cgccttcccc gagctcgcca tctctggtta cccgcccgag
     1201 gacctcctcc tgcggccggc gttcgtcgcc caggcgcaag agagtatgga gaaggtcgcc
     1261 gcccggaccg gccgggctac ggcggtcatc ggcttcccgc tgctcgagcg cgacctctac
     1321 aacgcggccg ccgtgtgcgc caacggcaag gtgcaaggcg tctaccgcaa gcacatcctt
     1381 ccgaactcca ctgtcttcga cgagcagcgc tacttcgccg cctcgaccac cgacgggccg
     1441 ctgttcaacg tggccggcgc tcgcgtcgcc gtgacggtct gcgaggacgc gtggagtccc
     1501 acgggcccga tcagcaccca ggcggccggt ggcgccgagc tggtggtcaa catcaacggg
     1561 tcgccgtact acgcgggccg gctgcgggag cgcgagacga tgctctcgac ccgcgccgcc
     1621 gacgcgcccg tgccgatcgc ctacgtcaac cttgtcggcg ggcaggacga gctcgtcttc
     1681 gacgggggct ccctcgtgtt cgacgaggtg ggccggctcg tggcgcgggc gcggcagttc
     1741 accgaggacc tgctcgtcgt cgacctcgac gtgcgcccca cgttccgcaa gcggatgctc
     1801 gacccgcgcg gccaccgccc ggcgccgacg ctcccggagg tcaaggtcag cgacgcgcac
     1861 ctgggcgagc ggcgcgagga gcctcgcatc gagccgacgc tcgcgccggt ccgcgaggtc
     1921 tacgaggccc tcgtcctcgg cactcgcgac tacgtcacca agaacggctt caccgacgcg
     1981 atcatcgccc tgtccggcgg catcgactcg tcgatcgtcg cgaccattgc gaccgacgcg
     2041 ctcgggcccg agcgtgtcct ctgtgtgctg atgccgtcgc ggttctcgag cgagggaagc
     2101 gtgaccgacg ccgaggccgt cgccgccaac ttgggcgtcc gcacgatcac cgtgccgatc
     2161 gaggcggcgc acgcgtcgtt cctcgacatg ctcgccgagc cgttcgccgg caccgagacc
     2221 ggcctggctg aggagaacct ccaggcccgg atccggggca cggtgatcat ggcgctgtcg
     2281 aacaagttcg gcgcgctggt actcacgacc ggcaacaaga gcgagctcgc cacgggctac
     2341 tcgacgctgt acggcgacat ggccggcggc ttctcggtca tcaaggacgt ccccaagacg
     2401 ctcgtgtacg cactcgcacg cgaccgcaac gagcgcgccg ggcgcgacct cgtcccggcc
     2461 gcagtgctcg acaagccgcc cagcgccgag ctgcggcccg accagcgcga cagcgactcg
     2521 ctgcccgagt actcgctact cgacccgatc ctcgagggtt acgtcgaggg cgatttgtcg
     2581 gtcgccgagc tcgaagaggc cgggttcgac cccgaggcgg tccgcaaggt cgcgaagctg
     2641 gtcgaccaca acgagtacaa gcggcggcag gcacctccgg gcgtgcgcgt gtcgcccaag
     2701 gccttcggca aggaccgccg cctgccgatc acgaatcgct ggcctggttg aacgggcgcg
     2761 tccgcgcctt ctacccggag ctggccctgg tcgtcgccag cttcttgtac ggcgcgacct
     2821 tcacgatcgt ccaggacgcg ctcgatcacc tcaccgcgac tggtttcatc ctgttgcgct
     2881 tcgcgatcgg cggcgccgcg ctcgctcccc tcgcgcttcg ccgagggtgg cgaggcccgg
     2941 cggcgcgccc gaacgactcc ggccgggtcc ttgccgggtg tggggtcgcc ctcggattta
     3001 ccgcgttc
//
LOCUS       DASPWO010000381         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1240304, whole genome shotgun sequence.
ACCESSION   DASPWO010000381 DASPWO010000000
VERSION     DASPWO010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240304"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2316)
                     /locus_tag="VEO00_08775"
     CDS             complement(<1..2316)
                     /locus_tag="VEO00_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYU58127.1"
                     /translation="MSRVRPVLGALFALALVAAACVAGPAGAPTARAGDAPKPNVVLI
                     LTDDQRFDTIGRCLNGFDPTDYDAGTDACMPNLQSLLMANGSTFQRGYVTTSLCCPSR
                     STILSGRYARHHGVTTNNDGEDLDDANTLPVWLDAAGYRTGLYGKYLNGYGYPDIPKW
                     PAGYVPPGWDSFHAFTDGQGYTDYDLTERDPGEQVSINHYALTGDDGTGACAADNDYS
                     TDRLCELALRFLNDPSTDPFFLFWTPASPHAPYEPPARWQAYANQVTIPPAGNLNKVA
                     TPNTPAWMNPEPLTAGELATQYQNFRSQLAMNRAVDDAIGRFNTALTDNGKLANTVWV
                     FLGDNGYAHGHHRLVGKNCEMEECHRVPFVVACPPAVCPGAVPDRVVQEALVLNLDLA
                     PTIVELAGATPTVPLDGRSLMPLLSNPAAPWREAFVMEDRNLETTGVTAWGSDGHLWS
                     YAKLPQGQQELYDLDVSADPWQLQNLWKDPDSATELADMQDRLAALTTPPVVTFTLTP
                     PPTTAETDADFAWSSSEPADFTCALDDAVPTPCGNDSTTGSRSFAALPGGPHRFSVSG
                     TDPEHNPGPAAVYPWMIGESGTPPNVTLTRPGPTDLLNGPAVKVTWTSSDDQGIARHD
                     VYERAGGPSAARSLVASTSPTVKTYSRSGTQGTTYCYDVTAVDVGGNSTTSGSGCAAV
                     PLDDVSLQPFGAVDRLRATDTYLSTVTKLNAAGEELRATVTGRRLGVLVKRTSSSGKL
                     QLWLDGAKVATIDLYSATTKSRVYVGVRSMTA"
     gene            complement(2313..>2624)
                     /locus_tag="VEO00_08780"
     CDS             complement(2313..>2624)
                     /locus_tag="VEO00_08780"
                     /inference="COORDINATES: protein motif:HMM:NF037701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58128.1"
                     /translation="TGAYQGTLMRMNAAGEAMTLPFTGRRVGVLARTTSSSGKMRVWV
                     DGALATTVDLYAAQTADRVYVYTASLSPASHTLRLAWDGGKNPASSGKVVLVDGVAVL
                     R"
BASE COUNT          353 a          901 c          962 g          408 t
ORIGIN      
        1 cgccgtcatc gagcggacgc cgacgtacac ccgggacttc gtggtcgcgg agtacaggtc
       61 gatcgtggcg accttcgcgc cgtccagcca gagctggagc ttccccgacg acgacgtccg
      121 cttgaccagg actcccagcc ggcgcccggt caccgtcgcc cgcagctctt ccccggccgc
      181 gttcagcttg gtcacggtgg acaggtacgt gtccgtggcg cgcaggcggt ccaccgcgcc
      241 gaacggctgc agcgagacgt cgtcgagcgg caccgcggcg caccccgacc cggaggtcgt
      301 ggagttcccg ccgacgtcca ccgccgtcac gtcgtagcag taagtcgtgc cctgggttcc
      361 cgaccgagag taggtcttca cggtcgggga ggtcgaggcg accaggctcc gcgccgcgga
      421 tggacctccg gcccgctcgt agacgtcgtg ccgggcgatg ccctggtcgt ccgacgaggt
      481 ccaggtcacc ttcacggccg ggccgttcag gagatcggtc gggccgggcc tggtcagggt
      541 gacgttcggc ggggtgccgc tctcgccgat catccacgga tacaccgccg ccggaccggg
      601 gttgtgttcg gggtcggtcc cggagaccga gaaccggtgc ggcccgccgg gcagggccgc
      661 gaacgaccgg gagccggtcg tgctgtcatt tccgcacggc gtgggaacgg cgtcgtcgag
      721 cgcgcaggtg aagtccgcgg gctccgagga cgaccaggcg aagtcggcgt cggtctcggc
      781 cgtcgtcggc ggaggcgtca gcgtgaacgt cacgaccggc ggcgtcgtca gcgccgccag
      841 ccggtcctgc atgtccgcca gctccgtcgc cgagtcgggg tccttccata agttctgcaa
      901 ctgccagggg tcggcgctca cgtcgaggtc gtacagctcc tgctggccct gcgggagctt
      961 cgcgtacgac cacaggtgcc cgtcggagcc ccaggccgtc acgccggtgg tctccaggtt
     1021 gcggtcctcc atgacgaagg cctcccgcca cggcgcggcg gggttcgaca gcagcggcat
     1081 gaggctccgc ccgtcgaggg gcacggtcgg cgtcgccccg gcgagctcga cgatcgtcgg
     1141 cgccaggtcg aggttcagga ccagggcctc ctggaccacc cggtcgggga cggcgccggg
     1201 gcagaccgca ggcgggcagg ccacgacgaa cggcacgcgg tggcactcct ccatctcgca
     1261 gttcttgccc accaggcggt ggtggccgtg cgcgtagccg ttgtcgccca ggaagaccca
     1321 tacggtgttc gcaagcttcc cgttgtccgt caatgccgtg ttgaaccgcc cgatcgcgtc
     1381 gtcgacggcg cggttcatcg ccagctgcga ccggaagttc tggtactggg tggccagctc
     1441 gccggccgtc agcggctctg ggttcatcca ggccggcgtg ttcggcgtgg ccaccttgtt
     1501 caggttcccg gccggaggga tcgtcacctg gttcgcgtag gcctgccagc gcgccggtgg
     1561 ctcatacggc gcgtgggggg atgcgggcgt ccagaagagg aagaacggat ccgttgacgg
     1621 gtcgttcagg aaccggagcg ccagctcgca gagccggtcg gtggagtagt cgttgtcggc
     1681 ggcgcacgcg ccggtgccgt cgtcgccggt gagcgcgtag tggttgatcg agacctgttc
     1741 gcccggatcg cgctcggtca agtcgtagtc ggtgtacccc tgcccgtcgg tgaaggcgtg
     1801 gaacgagtcc caccccggcg ggacgtatcc ggccggccac ttcgggatgt ccgggtagcc
     1861 gtacccgttg aggtacttcc cgtacaggcc ggtccggtag ccggcggcgt ccagccagac
     1921 cgggagcgtg ttcgcatcgt cgaggtcctc gccgtcgttg ttggtcgtca cgccgtggtg
     1981 gcgggcgtag cgcccggaca ggatcgtcga cctgctcggg cagcagaggg acgtggtgac
     2041 gtagccgcgc tggaacgtgc tcccgttcgc catcagcagg gactgcaggt tgggcatgca
     2101 cgcgtcggtc ccggcgtcgt agtcggtcgg gtcgaagccg ttcaggcagc gcccgatcgt
     2161 gtcgaagcgc tggtcgtcgg tcaggatcag cacgacgttc ggcttcggtg cgtccccggc
     2221 tcgggcggtg ggcgcgccag cgggtccggc gacgcacgcc gcggcgacca gggcgagcgc
     2281 gaacagggct cccaggacgg gacgaacccg gctcaccgca gcaccgccac gccgtccacg
     2341 agcaccacct tgccggacga cgcggggttc ttgcccccgt cccaggccaa gcgcagcgtg
     2401 tgggaggcgg gggagagcga cgccgtgtag acgtagaccc tgtccgccgt ctgggccgcg
     2461 tacaggtcca ccgtcgtcgc cagcgcgccg tccacccaca cccgcatctt ccccgacgac
     2521 gaagtcgtgc gggccaggac gcccacccgg cggccggtga acggcagcgt catcgcctcg
     2581 ccggcggcgt tcatccgcat cagggtgccc tggtacgcgc ctgt
//
LOCUS       DASPWO010000382         4581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_986100, whole genome shotgun sequence.
ACCESSION   DASPWO010000382 DASPWO010000000
VERSION     DASPWO010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986100"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..2235)
                     /locus_tag="VEO00_08785"
     CDS             complement(283..2235)
                     /locus_tag="VEO00_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HYU58129.1"
                     /translation="MTLFETRADPASVLAGLNEAQREAATAVRGPVAILAGAGTGKTT
                     TITHRIAYQVASGAFRAGEILAVTFTEKAAGELRGRLRALGVDGVEARTFHAAALSQL
                     GRWWERSTGAPLPEVLDTKARIVAPLANALPPPHKFLPRSDLAGEIEWAKNRMVPVER
                     YLDALAEAGHTPPIPADMMLRLYEGYERRKRAMRRFDFEDMLGLAVEMFDDHPDVAAE
                     LRSRYAAFTVDEYQDVNTLQAALLGRWLGGRDEVCVVGDDYQTIYSFTGASPEHLLRF
                     GDRYPDARIVKLEENYRSSPEVLTVANRLVPRMGGFPKMLRATRPSGPSPTLRWFPDQ
                     IAEIQFVVDEVRRLRGEGVAFEEMAVLYRINARSEPFEEAFAEARIPYQVRDGAFLRR
                     PGPRAVVHRLRRAGGPVVEEVERATDALGYAPGAEADSAEEATRQADLARMRALASEF
                     AAAHPDGDPAAFLAELAKRFAAEASARGVNLLTYHRSKGLEFDAVFLPRLLDGELPFR
                     SGRSRAPEDEERRLLYVGITRAKRHLYLSWPREPRTGRSPFVDEITGRPEDRGVPAPK
                     PVGAAKPVTVAADGPLYDRLKQWRMERSKADGVPAYVVFHDSTLAKIADSKPRTRLEL
                     LDVPGIGPAKLDRYGDEVLAVVGSAT"
     gene            complement(2288..3058)
                     /gene="lepB"
                     /locus_tag="VEO00_08790"
     CDS             complement(2288..3058)
                     /gene="lepB"
                     /locus_tag="VEO00_08790"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYU58130.1"
                     /translation="MAETKELLRAALQGIEPLGDGLDAVERRVASRRRRVRLEAAALA
                     LALTAGMAVVLLSVVHGGGGGAPVEGGPHVVGPTLEVEIPSGSMEPTLTVGDVVLVDE
                     GAYGTGIPERGDVIAFRLASDGPDWFFFKRVAGLPGDVVEERHGVIFVNGTEFSMPVA
                     ETPDRQTLGPWTVEPGHLFVVGDNLENSNDSRYPAIGQVPVKAVVGKVVEILEPGSRQ
                     GEVLPAPPAGVAPGPATPSPSVSLAPSPASTADESPAP"
     gene            complement(3051..3611)
                     /locus_tag="VEO00_08795"
     CDS             complement(3051..3611)
                     /locus_tag="VEO00_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU58131.1"
                     /translation="MGDPNVRAASGVIGVVEAVETAAPVSSAEDASFEEFFRAEYRSV
                     VRALALVTGDAGPAEDLAQEAFARALERWGRVGAMASPAGYVYRAALNLYRSRVRRIA
                     VGLRLRAQVSTPPDVGEQATLRAEVREMLRSLPPAQREALMLVEWLGYSAEEAAGILG
                     VRPASVRGRLFRARTSLRERFGGDDG"
     gene            complement(3622..4437)
                     /locus_tag="VEO00_08800"
     CDS             complement(3622..4437)
                     /locus_tag="VEO00_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019984486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HYU58132.1"
                     /translation="MKRGRRVASGLVAVVCLVATGPPAGAARQLPKPVPCEGCWRPAL
                     KTSWQWQLQGKVDRSVRAHVFDVDMFDTPAATVKALHRKGRVAVCYIDAGTWENWRPD
                     ADRFPKSVLGANNGWPGERWLDIRKRKVLGSILEDRMDLCASKGFDGVEFDNVDGYTN
                     HTGFPLTAANQLRFDVWLANQAHRRGLSAALKNDLEQIPTLLKYFDFALDEQCFQYSE
                     CGKLTRFVDAGKAVFEVEYSLPRSKFCPKANRMNFNSLRKRLALGAWRRICRS"
BASE COUNT          681 a         1756 c         1520 g          624 t
ORIGIN      
        1 caatcctccc ccccgtctca agaacgatgg gagcggctgc aagcttctct ctctccctct
       61 ccgatctcgg cccaaccacc acccttacac ggtaattgcc atcgaagtca cgttgggagc
      121 gtcgagcgat atctcgtcga cagccgagtg ctcgctcggt ggatccccgg aacctgcccg
      181 cacgccacga ggacgacgag gaggccgatc agaacgtgga cggggcgacg gatgcgcacg
      241 ctccgattgt agggaggccg acgagggaac cgacagagcc ccctacgtgg ccgaaccgac
      301 caccgccagg acctcgtcgc cgtagcggtc gagcttggcg gggccgatgc cggggacgtc
      361 gaggagctcg aggcgcgttc ggggcttgct gtcggcgatc ttggcgaggg tcgagtcgtg
      421 gaagacgacg tacgcgggga cgccgtcggc cttcgagcgc tccatccgcc actgcttgag
      481 gcggtcgtag agcggaccgt cggcggcgac ggtgacgggc ttcgcggctc caacgggctt
      541 cggcgccggg accccgcggt cctcggggcg gccggtgatc tcgtcgacga aggggctgcg
      601 gcccgttcgg ggttcgcggg gccatgacag gtacaggtga cgcttggccc gcgtgatccc
      661 cacgtagagc agccggcgtt cctcgtcctc gggagcgcgg ctgcggcccg agcggaacgg
      721 gagctcgcca tccagcaggc gtggcaggaa taccgcgtcg aactccaggc ccttggagcg
      781 gtgatacgtg aggaggttca cgccacgggc cgacgcctcc gccgcgaacc gcttcgccag
      841 ctcggccagg aaggcggccg ggtcgccgtc cggatgtgcg gcggcgaact cggacgccag
      901 cgcgcgcatc cgggccaggt cggcctgccg ggtggcctcc tcggccgagt cggcctccgc
      961 cccgggcgcg tagcccaggg catcggtggc tcgttcgacc tcctccacga ccgggccgcc
     1021 ggcccgccgg agccggtgca ccacagctcg gggtcccggg cgccgcagga acgcaccgtc
     1081 ccggacctgg tacgggatgc gcgcctcggc gaaggcctcc tcgaacggct cggagcgggc
     1141 gttgatccgg tacaacacgg ccatctcctc gaaggccacg ccctcaccgc gcaggcgccg
     1201 gacctcgtcc acgacgaact ggatctccgc gatctggtcc gggaaccacc gcagggtggg
     1261 cgacggcccc gaggggcggg tggcgcgcag catcttgggg aacccgccca tccgcgggac
     1321 cagccggttc gccacggtca gcacctcggg cgacgagcgg tagttctcct cgagcttcac
     1381 gatccgcgcg tccgggtacc ggtcgccgaa ccgcaacagg tgctcgggcg acgcgccggt
     1441 gaacgagtag atcgtctggt agtcgtcgcc gaccacgcag acctcgtcgc gtccgccgag
     1501 ccaccggccg agcagcgccg cctgcagcgt gttcacgtcc tggtactcgt ccacggtgaa
     1561 cgccgcgtac ctcgaccgga gctccgccgc cacgtccggg tggtcgtcga acatctccac
     1621 ggccagcccg agcatgtcct cgaagtcgaa ccggcgcatg gcccgcttgc ggcgctcgta
     1681 gccctcgtac agtcggagca tcatgtcggc ggggatcggc ggcgtgtggc ccgcctctgc
     1741 cagcgcgtcg agataccgct ccaccggcac catccggttc ttggcccact cgatctcgcc
     1801 ggccaggtcg ctccggggca ggaacttgtg cggcggaggc aacgcgttcg ccaggggcgc
     1861 cacgatccgg gccttggtgt cgaggacctc gggaagtggc gctccggtgg accgctccca
     1921 ccaccgcccc agctgcgaca gcgccgccgc gtggaacgtc cgcgcctcca cgccgtccac
     1981 cccgagcgcc cgcagccgcc cgcggagctc gcccgccgcc ttctccgtga acgtcacggc
     2041 caggatctcg cccgcccgga acgcgcccga ggccacctgg tacgcgatcc ggtgcgtgat
     2101 cgtggtggtc ttgccggtcc cggcccccgc caggatcgcc accggccccc gcacggccgt
     2161 cgccgcctcc cgctgggcct cgttgagccc ggccagcacc gacgcggggt cggcgcgtgt
     2221 ctcgaacagg gtcatcgccg cggatcgtag caacgcccgg cgacacggcc gtccggcagt
     2281 gccgtgatca gggagccggg gactcgtccg ccgtggaggc cgggctgggc gcgagcgaga
     2341 ccgacggact gggcgtggcg ggacccggag caaccccggc cggcggcgcc gggagaacct
     2401 cgccctgacg ggatcccggc tcgaggatct cgacgacctt tccgaccacg gccttcaccg
     2461 ggacctgccc gattgcgggg taccgggagt cgttcgagtt ctcgaggttg tcgcccacca
     2521 cgaacaggtg ccccggttcg actgtccacg gcccgagcgt ctgccggtcg ggcgtctccg
     2581 cgaccggcat cgagaactcc gtcccgttca cgaatatcac cccgtgccgt tcctccacca
     2641 cgtcgccggg caggcccgcc acacgcttga agaagaacca gtcagggcca tccgaggcca
     2701 gacggaacgc gatcacgtcc ccgcgttccg ggatccccgt gccgtaggcg ccctcgtcca
     2761 ccagtaccac gtcgcccacc gtcagcgtcg gctccattga ccccgagggg atctccacct
     2821 cgagggtcgg ccccactacg tgcgggccac cctcgaccgg cgccccgcct ccaccgccat
     2881 gcacgaccga cagcaggacc accgccattc cggccgtgag ggccagggcc agcgccgccg
     2941 cctccagccg cacccgtcgc cggcgagagg ccacccgccg ctccacggca tccagcccgt
     3001 cacccagcgg ctcgatcccc tgcagcgccg ctcgcagtaa ttccttcgtc tcagccatcg
     3061 tcgcctccga aacgctcccg aagggaggtc ctggcccgga acagccgccc ccgaaccgag
     3121 gccggacgga cccccaggat cccggcggcc tcctcggccg agtaccccag ccactccacc
     3181 agcatcaggg cctcccgctg ggccggaggg agcgaccgga gcatctcccg gacctcagcg
     3241 cggagggtgg cctgctcgcc gacgtccggc ggggtgctca cctgggcccg gagccggagc
     3301 cccaccgcga tccgtcgcac acggctccgg tacaggttca gcgccgcccg gtacacatac
     3361 ccggcgggcg acgccatcgc gccgacccgc ccccaccgct ccagggccct ggcgaacgcc
     3421 tcctgcgcca ggtcctcggc cggcccggcg tcgccggtga ccagcgccag cgcccggacc
     3481 accgaccggt actcggcccg gaagaactcc tcgaacgacg cgtcctcggc cgagctcacc
     3541 ggagccgccg tctccaccgc ctccacgaca ccgatgacgc ccgaggccgc ccgaacgttt
     3601 gggtcgccca tcgggatcgg ctcaggaccg gcagatccga cgccacgccc cgagcgccag
     3661 ccgcttccgc aacgagttga agttcatccg gttcgccttc ggacagaact tcgaccgcgg
     3721 caacgagtac tccacctcaa acacggcttt cccggcgtcc acgaagcgcg tcagcttccc
     3781 gcactccgag tactggaagc actgctcgtc gagcgcgaag tcgaagtact tcagcagcgt
     3841 cgggatctgt tcgaggtcgt tcttcagcgc cgccgacagc cctcgccggt gagcctggtt
     3901 cgccagccac acgtcgaacc gcagctggtt cgccgcggtg agggggaacc cggtgtggtt
     3961 cgtgtacccg tcgacgttgt cgaactccac accgtcgaac cccttggagg cgcacaggtc
     4021 catgcggtcc tcgaggatcg accccaggac cttccgcttg cggatgtcga gccaccgttc
     4081 gccgggccat ccgttgttcg cgccgagcac ggacttgggg aaccggtcgg cgtccggccg
     4141 ccagttctcc cacgtccccg cgtcgatgta gcacacggct acgcgaccct tgcggtgcaa
     4201 cgccttcacg gtcgccgcgg gcgtgtcgaa catgtccacg tcgaacacgt gcgcccgcac
     4261 ggaccggtcg accttcccct ggagctgcca ctgccacgac gtcttcaggg ccggacgcca
     4321 gcacccctcg cacggaaccg gcttcggcag ctgcctcgcg gcgccggcgg gcggaccggt
     4381 cgcaaccagg cacacgacgg ccacgagccc agacgcgacc cgccgacccc gtttcacgcg
     4441 ggtcaagggt aggcttggcc ggtgactcgc gcgtccacca tcggcgacct ccgggcggcc
     4501 gggtaccccg accggacggt gaaggaggag ctccgggcga acctcctggc gaagctcggg
     4561 cggggcgaga acctgttccc g
//
LOCUS       DASPWO010000383         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1240447, whole genome shotgun sequence.
ACCESSION   DASPWO010000383 DASPWO010000000
VERSION     DASPWO010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240447"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VEO00_08805"
     CDS             <1..395
                     /locus_tag="VEO00_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HYU58133.1"
                     /translation="FDAVHSAKARGDDVLIVDTAGRLHTRQPLMAELAKVKRVIEKAA
                     GDVEEVLLVIDATTGQNGIAQAREFTNAVPLTGVALTKLDGTAKGGIVLAVRDQIGVP
                     VKLVGTGEGLRDLQPFDPRAFADNLLAG"
     gene            429..683
                     /locus_tag="VEO00_08810"
     CDS             429..683
                     /locus_tag="VEO00_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HYU58134.1"
                     /translation="MARRTQITLEDEQYDLLRRVSATTGLSMAELIRRALDRTYARRG
                     VEALDATFGMWRDRSNIDDLMKRLRPGLGKRLVKDRGGAR"
     gene            667..1059
                     /locus_tag="VEO00_08815"
     CDS             667..1059
                     /locus_tag="VEO00_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYU58135.1"
                     /translation="MAALADTTILVDHLRGRREAKDLLRGEIAAGEPLFASVLIRVEL
                     LAGARPSDRPRLDQLIGSFIWIDVTAAIADRAGEFANRYARSHRNIDVVDYVIAATRE
                     ELGVPLWTLNVRHFPMLPDLAPPYGPAG"
     gene            complement(1208..2101)
                     /locus_tag="VEO00_08820"
     CDS             complement(1208..2101)
                     /locus_tag="VEO00_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58136.1"
                     /translation="MDTLETRIREALDRSVGAPPQRSMPPGTRSRVRRRQARWVVGIV
                     VVAVGAVFGGLTALKAIPGGGGGAPGEGGTFVPNHPLEFVPEGWPTVRIGDPADGFTL
                     PSEVAGAAGPVRVIASGTVDGHGFSFQSYVGDPRAGTSGPCLGFAGPGLDRFASPDPQ
                     PPGAVGGISSSTCATDQGVPQSLDMYRTSQQDTDQAPGTAPNYGFLSARVDRLGLRLD
                     DGSTVDIPVLPSPPGWEGMQAFLFFPPVGSSGTLTAYAADGTELARAPMCSFSEDAAF
                     GCGGGGTTTQLAPVPTPSAPS"
     gene            complement(2101..2607)
                     /locus_tag="VEO00_08825"
     CDS             complement(2101..2607)
                     /locus_tag="VEO00_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU58137.1"
                     /translation="MTEIGELGVAREVGLEALYRRLVGGAEGLARALTGDPYLAQDIA
                     HDAFIRVAGRFGHLRNAGAFEAYLRRAVVNQCRSHFRRAGVERRGLQRLGVRIEASAA
                     HALEERDSLWATIQALPYRQRAAIVLRFYEDLSEAQAGEILRCSPRAVNSLVSRAMAT
                     LRAEMEER"
     gene            2668..>3409
                     /locus_tag="VEO00_08830"
     CDS             2668..>3409
                     /locus_tag="VEO00_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HYU58138.1"
                     /translation="MFDTLTKRFDAVFTKLRSRGKLHPKQVDSALGDIRTALLEADAA
                     VEVVDDFLARVRARSLGDEVMKSLTPAQQVVKIVRDELTETLGGTHTPFTLPGANPAV
                     IMMAGVQGSGKTTHCAKLALHFKEKGKHPLLVAADLQRPAAIEQLFTLGAEIGVPVVS
                     EGKEPEKVARNALGEAKRRGLDVVIVDTAGRLHVDAEMMKQARRVRDTIKPHQVLMAC
                     DSMTGQDAVVQARAFMQEVDTTGFVLTKL"
BASE COUNT          522 a         1230 c         1180 g          477 t
ORIGIN      
        1 cgttcgacgc ggtgcactcc gcgaaggccc ggggcgacga cgtcctgatc gtcgacacgg
       61 ccggccgcct gcacacccgc cagcccctga tggccgagct ggccaaggtc aagcgggtca
      121 tcgagaaggc cgccggcgac gtggaggagg tgctgctggt gatcgacgcc accaccggcc
      181 agaacggcat cgcccaggcc cgggagttca cgaacgccgt gccgctgacc ggcgtcgccc
      241 tcaccaagct cgacggcacc gcgaagggcg gcatcgtgct cgccgtccgc gaccagatcg
      301 gcgtccccgt caagctcgtc gggaccgggg agggcctgcg cgacctccag ccgttcgatc
      361 cccgggcctt cgccgacaac ctcctcgccg gttgaggccg gggtcccgcg atgatgtagc
      421 attacatcat ggcgaggcga acccagatca cgctggagga cgagcagtac gatctgttgc
      481 ggagggtttc cgcgacgacc ggcctttcca tggccgagct catccgtaga gcgctcgacc
      541 ggacctacgc ccgacgcggc gtcgaggcgc tggatgcgac gttcggcatg tggcgcgacc
      601 ggagcaacat cgacgacctg atgaaacggt tgcgccccgg attggggaag cgcctcgtga
      661 aggaccgtgg cggcgctcgc tgacacgacg atcctcgtcg atcacctccg cggccgacga
      721 gaggccaagg atctccttcg tggcgagatt gccgcaggcg aaccattgtt cgcgtcggtc
      781 ctcattcgag tcgagttgct ggccggcgcc cgcccgtcgg acaggccacg cctggaccaa
      841 ctgattggct cgttcatctg gatcgacgtg accgcggcca tcgccgaccg cgccggggag
      901 ttcgcgaatc gatacgcgcg ctcccatcgg aacatcgatg tcgtcgacta cgtcatcgcg
      961 gcgacccgtg aggagttggg cgtcccccta tggacactca acgttcgcca cttcccgatg
     1021 ctgcccgacc tcgccccgcc ctacggcccc gccggctgaa cgatccccgc cgcgccaaca
     1081 cgggtgacat cgttcgcgtg tgttgccgag ccttggcccg atctctggac tgctcggcga
     1141 acagagcgat tctgacgaca tcgggaccca ctcgcccgtc gcgcggctgc ctcgatccgt
     1201 cgcatgacta ggagggtgcc gacggcgtcg ggaccggcgc aagttgggtg gtggttcccc
     1261 ctcctccgca tccgaaggcg gcgtcctcgg agaacgagca catcggcgct cgcgccagct
     1321 cggtcccgtc ggccgcatat gcggtcagcg tgccgctgct gcccacgggc gggaagaaca
     1381 ggaaggcctg catgccctcc caccccggcg ggctcggcag cacagggatg tcgaccgtgc
     1441 tcccgtcgtc gaggcgcagc ccgagccggt ccacccgggc gctgaggaac ccgtagttcg
     1501 gggccgtgcc gggggcctga tcggtgtcct gctggctggt ccggtacatg tccaggctct
     1561 ggggcactcc ctgatctgtc gcacacgtgg aagacgagat cccgcccacc gcccccggcg
     1621 gttgcggatc cggggaggcg aaccggtcca ggcccgggcc cgcgaatccc aggcaggggc
     1681 cgctcgttcc ggccctgggg tcgcccacgt acgactggaa cgagaaccca tggccgtcga
     1741 ccgtcccgga cgcgatgacg cgcaccggtc cggcggcgcc cgcgacctcg gacggcaacg
     1801 tgaagccgtc cgcggggtcg ccgatgcgca cggtgggcca cccctcgggg acgaactcga
     1861 gcggatggtt cggcacgaac gtcccgccct ccccgggcgc cccgccgccg cccccgggga
     1921 tcgccttcag cgcggtgagt cccccgaaca cggctcccac cgcgacgacg acgatgccga
     1981 cgacccaccg ggcctggcgc cgccgcaccc gcgaacgggt gcccgggggc atcgaccgct
     2041 gcgggggagc accgacgctc cggtccaggg cctcgcggat cctcgtctcg agcgtgtcca
     2101 tcagcgttcc tccatctctg ctcgtagcgt ggccatggcc cgcgacacca gggagttcac
     2161 cgcgcgcggc gagcaccgca ggatctctcc ggcctgtgcc tccgagaggt cctcgtagaa
     2221 ccggaggacg atcgccgcgc gctggcggta cggcagcgcc tggatcgtcg cccacagcga
     2281 gtcccgttcc tcgagcgcgt gggcggccga ggcttcgatc cggacgccca gccgctggag
     2341 accccggcgc tcgacgcctg cccgtcggaa gtgcgagcgg cactggttga cgaccgcgcg
     2401 gcggaggtac gcctcgaacg ccccggcgtt gcggagatgc ccgaaccgtc ccgcgacgcg
     2461 gatgaacgcg tcgtgggcga tgtcctgggc caggtacggg tcgccggtga gggcgcgggc
     2521 cagcccctcg gcgcccccca ccaggcgccg gtacagcgcc tccagcccaa cctcccgcgc
     2581 cacgccgagc tctccgatct cggtcatcgg tgtgaagacg cgccaggggg cggccacgct
     2641 tcgcccggtc gatacactgg ccgcctgatg ttcgacacgc tcaccaagcg gttcgacgct
     2701 gtcttcacca agctccggtc gcgggggaag ctccatccga agcaggtgga ttcggcgctg
     2761 ggcgacatcc ggacggcgct gctcgaggcc gacgcggccg tggaggtcgt ggacgacttc
     2821 ctggcgcggg tccgggcacg ttcgctgggc gacgaggtca tgaagtccct cacgccggca
     2881 cagcaggtcg tgaagatcgt ccgggacgag ctcaccgaga ccctcggcgg cacgcacacg
     2941 ccgttcacgc tgccgggggc gaacccggcc gtgatcatga tggccggcgt gcagggctcc
     3001 gggaagacca cccactgcgc caagctggcc ctgcacttca aggagaaggg caagcacccg
     3061 ctgctggtcg cggcggacct ccagcggccg gcggccatcg agcagctgtt cacgctgggg
     3121 gccgagatcg gcgtgccggt cgtctccgag ggcaaggagc cggagaaggt cgcgaggaac
     3181 gccctgggcg aggccaagcg gcgcggcctc gacgtggtca tcgtcgacac cgcggggcgc
     3241 ctccacgtgg acgccgagat gatgaagcag gcccggcggg tcagggacac gatcaagccg
     3301 caccaggtcc tcatggcctg cgactccatg acgggccagg acgccgtggt ccaggcccgg
     3361 gcgttcatgc aggaggtcga caccaccggt ttcgtgctaa cgaagctcg
//
LOCUS       DASPWO010000384         5045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_477456, whole genome shotgun sequence.
ACCESSION   DASPWO010000384 DASPWO010000000
VERSION     DASPWO010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477456"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VEO00_08835"
     CDS             complement(<1..1238)
                     /locus_tag="VEO00_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58139.1"
                     /translation="MASVRGQVAVTPPGDDARPADVEVGDEALSIAFEGGETVTLPFV
                     DIDDLHDDDYTLRLTDFTGRRLDLSMLGSAYGQILEDVRERRNEALQRDLLLTGVNLQ
                     DTFPGKVFGGADPMPAELRLFEDQLVVMPERGRMWGLPFSFVDAVDWDQELYQVRVRA
                     DDGTTNVFGHLAKRSEEFRDELNRLLAALAKRTAATLLSLLPGADPGALDRLAAVMRD
                     GRAVQQRVVEAIDPALWPKLEDAVVGTADLRQTYEWLKARGPSGWPALGVKAVRGKAD
                     EGPAAPAEAEATEAAPTEAAPTASVLWFFVPFEGRNAVAQEITSEAGHATYLFRPVEP
                     EAFAGLSGDAMAEAVAVGVARLNRALLTLNFRREPIYASDEEIATGRFSRYRVALRVL
                     DHLRFARRQFLGRAIHNETW"
     gene            complement(1239..1880)
                     /locus_tag="VEO00_08840"
     CDS             complement(1239..1880)
                     /locus_tag="VEO00_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYU58140.1"
                     /translation="MTVPIPFTDNYTDHSTELGYQFEFFCERCGNGYKSGFNASAVGI
                     GGKIARGLGGMFGGALGNIGVGADQAKDLTESKAKDDALRKAVEEMRPLFNQCHKCGQ
                     WVCKEICWNEEFQLCVNDAPKLEQELAGMQSARRLQQVQEKVGQADYASDVNVERKQV
                     ALCPNCGAEAPPSGKFCANCGQALDMPVTCGKCGSESPRGTKFCPECGNNLAA"
     gene            complement(1975..2316)
                     /locus_tag="VEO00_08845"
     CDS             complement(1975..2316)
                     /locus_tag="VEO00_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58141.1"
                     /translation="MNTARPSLLRRVLALVAFGLLAGGVVATGASLVSRDLCGDEVGA
                     AAVRACDDLVRTLSIRTGLLAGVVSVLMGLLVLGLIRTARQDEEDRRIRRREDLEALQ
                     GRIPPASAGER"
     gene            complement(2313..2963)
                     /locus_tag="VEO00_08850"
     CDS             complement(2313..2963)
                     /locus_tag="VEO00_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYU58142.1"
                     /translation="MLDRFTWFKQSAFRYDGDGITVYFDAWGIPDDAPPADVLFVSHG
                     HFDHFDRDDLARIRSDATTIVCPRDLAAELSGNVIAVAPGDDVDVSGIKGQAVPAYNI
                     AADRLDFHPKSNGWVGYVLTLDGFTVYHAGDTDHTSDLSSIRADAALLPIGGHYTMDA
                     DEAAGLVHEIRPGTAVPMHYGFLEDTAPASASDRFRAAAEPTEVHVFDPVNPFEHT"
     gene            3100..4425
                     /locus_tag="VEO00_08855"
     CDS             3100..4425
                     /locus_tag="VEO00_08855"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU58143.1"
                     /translation="MEFRESAAAGLPQRGLRSTFRLLRRNPAFTRLYAAQLISMAGDW
                     FLIVALYGLALELTGSALAASGVLVAHLVPFFLVSPFGGVLADRLDRRRLMIAADVLR
                     AGVCVGFLVIDRSGEIWLMFVLIAGLSSLNALFDPAATAAVPNLVDPEDLPAANVLSG
                     SAWGAMLAVGAALGGLIAGVFGRETAFLVDAGSFVASAALLLTVRRAFSEAREGEHPS
                     LRAAIVETVRYARGDRRVTALLAVKGGFGLGGGVIGLLPVFATQVFGGGDGAIGVLLA
                     ARGLGALVGPFLARRWVRNEDHRLFSAIASAMALFGVFYLVFPLAGSLAVAAPLAAGA
                     HLGGGAQWTLSTYGLQVVTPDRIRGRVFAFDFMLVTASMTVSSLLAGWAAGHAGPRVV
                     MAVLAAVALAYALIWAALTKRLRAGLRAGDSLRSSYEGAFGGAGGDPPA"
     gene            complement(4380..>5045)
                     /locus_tag="VEO00_08860"
     CDS             complement(4380..>5045)
                     /locus_tag="VEO00_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HYU58144.1"
                     /translation="GIVAYNRFVSQRQLVRNAYANIDTELRRRYDLIPNLVETVRGYA
                     EHERQALEAVIRARQQAIQVQGSPAAQAQAENILTQALRQLFAVAEAYPNLKANENFL
                     ALQAELSNTEDRIQTSRRFYNGNVQDYNRRVQSFPSTIIARMFGFKEEEFFEIEEQLR
                     EAGAPRVDFTGVGQPGGPPTPGVTPPAGGPPPAAGPPPAMPPAAPAPQAGGSPPAPPN
                     APS"
BASE COUNT          643 a         1788 c         1810 g          804 t
ORIGIN      
        1 tcccatgtct cgttgtgaat ggcgcgcccg aggaactgcc gccgggcgaa gcgcaggtgg
       61 tccaggacgc gcagcgccac ccggtaccgg ctgaagcggc ctgtcgcgat ctcctcgtcg
      121 ctggcgtaga tcggctcgcg ccggaagttc agcgtgagca gcgcgcggtt caggcgagcg
      181 acgccgacgg ccaccgcctc ggccatcgcg tcaccggaca gaccggcgaa cgcctctggc
      241 tccaccggac ggaacaggta cgtcgcgtgc ccggcctcgg aggtgatctc ctgcgcaacg
      301 gcgttgcggc cttcgaacgg gacgaagaac cacaggaccg aggcggtggg ggcggcctcg
      361 gtgggggcgg cctcggtggc ctcggcctcg gccggcgccg ccggcccctc gtcggccttg
      421 ccgcggacgg ccttcacgcc gagggcgggc cagcccgagg ggccgcgggc cttcagccac
      481 tcgtaggtct ggcgcaggtc cgcggtgccg acgaccgcgt cctcgagctt cggccagagc
      541 gccgggtcga tcgcctcgac gacgcgctgc tggacggcgc ggccgtcgcg catcaccgcc
      601 gccaggcggt cgagcgcgcc cggatcggcc cccggcagca gcgacaacag ggtggccgcc
      661 gtgcgcttcg ccagggcggc gagcaagcgg ttcagctcgt cgcggaactc ctcggatcgc
      721 ttggccaggt ggccgaagac gtttgtagtg ccgtcgtcgg cgcgcacgcg cacctggtac
      781 agctcctggt cccagtcgac cgcgtcgacg aacgagaacg gcaggcccca catccgcccg
      841 cgctcgggca tcaccaccag ctggtcctcg aacagtcgca gctcggccgg catcgggtcg
      901 gcgcccccga acaccttgcc ggggaaggtg tcctggaggt tcacgccggt cagcagcagg
      961 tcccgctgca gcgcctcgtt ccggcgttcc cggacgtcct cgaggatctg gccgtacgcg
     1021 ctcccgagca tcgagaggtc gagccgccgc ccggtgaagt cggtcaggcg cagcgtgtag
     1081 tcgtcgtcgt gcaggtcgtc gatgtcgacg aacggcagcg tgacggtctc gccgccttcg
     1141 aacgcgatcg agagggcttc gtcaccgacc tcgacgtccg cgggacgcgc gtcgtcgccg
     1201 ggtggggtga ccgccacctg cccccggacg gaagccacct atgccgcgag gttgttgccg
     1261 cactccggac agaacttcgt cccgcgcggc gactcgctgc cgcacttgcc acaggtcacc
     1321 ggcatgtcca gcgcctgtcc gcagttcgcg cagaacttgc cggacggcgg ggcctcggcg
     1381 ccgcagttcg ggcacagcgc gacctgcttg cgctcgacgt tcacgtcgct cgcgtagtcg
     1441 gcctggccca ccttctcctg tacctgctgc agccggcggg cggactgcat gcccgccagc
     1501 tcctgctcga gcttcggcgc gtcgttcaca cagagctgga actcctcgtt ccagcagatc
     1561 tccttgcaca cccactgtcc gcacttgtgg cactgattga agagcggtcg catctcctcg
     1621 acggccttgc gcagggcgtc gtccttggcc ttcgactcgg tgaggtcctt cgcctggtcg
     1681 gccccgaccc cgatgttgcc gagcgcgccg ccgaacatcc cgcccaggcc ccgcgcgatc
     1741 ttccccccga tcccgaccgc cgacgcgttg aagcccgact tgtagccgtt cccgcatcgc
     1801 tcgcagaaga actcgaactg gtagccaagc tcggtggagt ggtcagtgta gttgtcggtg
     1861 aacgggatcg ggaccgtcat gtcggcgcct cctcagtcgt gcgtgccgtt acgggggcca
     1921 cgcggaacgc ggactgtaca ccgcttcgtc ccgtcgggaa atggtccgaa cggactatcg
     1981 ttcgccggcc gaggccggcg ggatgcgccc ttgcaacgcc tcgaggtctt cacgccggcg
     2041 gatgcggcgg tcctcctcgt cctggcgggc cgtacggatc aaccccagga ccaggagtcc
     2101 catcagcacg gaaaccacgc cggccaggag accggtccgg atcgagagcg tccggaccag
     2161 gtcgtcgcag gcccggacgg ctgccgcgcc gacctcatcc ccgcacaggt cccgcgaaac
     2221 gagcgatgcc ccggtggcca cgacgccgcc ggccagcagg ccgaacgcga cgagggcgag
     2281 gacgcggcgg aggagactcg gccgtgcggt gttcacgtgt gctcgaaggg gttgaccgga
     2341 tcgaacacgt gcacctcggt gggctccgcg gcggcccgga accggtccga ggctgaggcg
     2401 ggagcggtgt cctccaggaa cccgtagtgc atcggcaccg cggtgccggg ccggatctcg
     2461 tgcacgagcc cggccgcctc gtcggcgtcc atcgtgtagt ggcctccgat cgggagcagg
     2521 gccgcgtccg cccggatcga cgacaggtcg gacgtgtgat cggtgtctcc ggcgtggtac
     2581 acggtgaagc cgtcgagggt cagcacgtag ccgacccatc cgtttgactt cgggtggaag
     2641 tccagccggt ccgcggcgat gttgtaggcg ggaaccgcct gccccttgat ccccgacacg
     2701 tctacgtcgt ccccgggcgc cacggcgatc acgtttccgg agagctcggc ggcgaggtca
     2761 cgcggacaga cgatcgtggt cgcgtcggat cggatccggg cgagatcgtc gcggtcgaaa
     2821 tggtcgaagt gtccgtggga gacgaacagc acgtcggccg gcggcgcgtc gtccgggatc
     2881 ccccacgcgt cgaagtagac cgtgatgccg tccccgtcgt aacgaaacgc ggactgcttg
     2941 aaccacgtga accggtcgag catcgcttcc tcctctcgtc gacccgaggg ctcccctgct
     3001 ccggcgtccc cttccaacct agccccggca tcgaggcccg aacaacccgt ttcgttcccg
     3061 gggagagccc ggaccatgct tggtaccctc gcccggtcca tggaattccg cgaatcggca
     3121 gccgccggat tgccgcagcg cggcctgcgg tcgacgttcc ggctgctccg ccgcaacccc
     3181 gccttcacca ggctctacgc cgcacagctg atctcgatgg ccggcgactg gttcctgatc
     3241 gtcgcactgt acgggctggc gctagagctg accggttccg cgctcgccgc gtcgggcgtg
     3301 ctggtggcgc acctggtccc gttcttcctg gtcagcccct tcggcggggt gcttgccgac
     3361 cgcctggacc gccggcggct gatgatcgcc gccgatgtcc tgcgcgccgg tgtctgcgtc
     3421 gggttcctgg tgatcgaccg gagcggcgag atctggctga tgttcgtcct gatcgccggg
     3481 ctgtcgtcgc tgaacgccct gttcgaccct gccgccaccg ccgccgtccc caacctggtc
     3541 gacccggagg acctgcccgc agcgaacgtg ctctccggtt cggcctgggg ggcgatgctc
     3601 gcggtggggg cggccctcgg cggcctgatc gccggggtct tcggccggga gaccgccttc
     3661 ctggtggacg ccgggtcctt cgtcgcgtcc gcggcgctgc tgctcactgt gcggcgggcg
     3721 ttctccgagg cgcgcgaggg ggaacacccc tcgctgcggg cggcgatcgt ggagaccgtg
     3781 cgctacgcgc gaggcgaccg ccgcgtcacc gccctgctgg cggtgaaggg aggcttcggg
     3841 ctcgggggtg gcgtgatcgg gctgctgccg gtgttcgcca cccaggtgtt cgggggcggg
     3901 gacggagcga tcggggtcct gctggcggcc cgcgggctcg gcgccctggt cgggcccttc
     3961 ctcgcccggc gctgggtgcg caacgaggac caccggctgt tcagcgcgat cgcctcggcg
     4021 atggcgctct tcggggtctt ctacctggtc ttcccgctgg ccgggtcgct ggcggtcgcc
     4081 gcaccgctgg cggccggggc gcacctcggc ggcggcgcgc agtggacgct ctccacgtac
     4141 gggctgcagg tggtcacacc cgaccggatc cgcggccggg tgttcgcgtt cgacttcatg
     4201 ctcgtcacgg cgtcgatgac cgtgtcgagt ctgctcgccg gctgggcggc cgggcacgcc
     4261 ggcccgcgcg tggtgatggc cgtcctggcc gcggtcgcgc tcgcctacgc gctgatctgg
     4321 gccgcgctca ccaagcgcct gcgcgccggc ctgcgcgcgg gcgactcgct gcgatcgtcc
     4381 tacgaaggcg cgttcggcgg agccggcggc gacccgcctg cctgaggggc gggagcggcg
     4441 ggcggcatgg ccggcggcgg accggcggcg ggtggaggtc cgcccgccgg gggggtgacg
     4501 ccgggggtcg gcgggccgcc gggctggccc acgccggtga agtccacgcg gggcgcgccg
     4561 gcctcccgga gctgctcctc gatctcgaag aactcctcct ccttgaaccc gaacatcctg
     4621 gcgatgatgg tcgaggggaa ggactggacg cgccggttgt agtcctgcac gttgccgttg
     4681 tagaagcgcc gcgatgtctg aatgcggtcc tcggtgttgg acagctccgc ctgcagcgcc
     4741 aggaagttct cgttcgcctt caggttcggg taggcctcgg ccaccgcgaa cagctggcga
     4801 agggcctggg tcaggatgtt ctccgcttgg gcctgggcgg ccggcgagcc ctgcacctgg
     4861 atcgcctgct gccgcgcgcg gatgacggcc tcgagcgcct gccgctcgtg ctcggcgtag
     4921 ccacgcacgg tctcgaccag gttcgggatc aggtcgtacc gccgccgcag ctcggtgtcg
     4981 atgttcgcgt aggcgttgcg cacgagctgc cgctgggaga cgaagcggtt gtaggcgacg
     5041 atccc
//
LOCUS       DASPWO010000385         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1240588, whole genome shotgun sequence.
ACCESSION   DASPWO010000385 DASPWO010000000
VERSION     DASPWO010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240588"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VEO00_08865"
     CDS             complement(<1..325)
                     /locus_tag="VEO00_08865"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HYU58145.1"
                     /translation="MEAEASMAGARQMAGFKPFYQFSSPTRVIAGEGLIESAGFEFAK
                     EGAARVMVVTDAVIRDTGIPGRAAEGIEGGGLEVAGTFDEVPQDSDTEVVARCAEAAR
                     DAGADS"
     gene            complement(350..766)
                     /locus_tag="VEO00_08870"
     CDS             complement(350..766)
                     /locus_tag="VEO00_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HYU58146.1"
                     /translation="MAYFKDAQDVYDTIGKLFVDLSQDDELAPKFNKANAIVRYEFTD
                     PDSAITTRLQEGEPGQVDFGDSEMEPDVTMSMEADTAHRFWLGKVNVTVAMARGQIKA
                     KGPVAKILKLVPLTKPVFPRYKAQLEQQGRDDLVNV"
     gene            complement(898..1827)
                     /locus_tag="VEO00_08875"
     CDS             complement(898..1827)
                     /locus_tag="VEO00_08875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HYU58147.1"
                     /translation="MKIGVALPTVGLDHKPDQLLEVAETAERLGFDSVWATDHALMSY
                     ARQSEYPYGRSGTEMAMTPGIRWLEPLSTLAFVASRTERVRLGTSVLVLPYRNPIIVA
                     AEAATVHLLSGDRLILGVGAGWMREEFAALGLDAAERGARLDEYIEVLRTLWRDDPAS
                     FHGRFVDFDDVVLAMTPRGAEPPVWVGGNSRPALRRALRQGDGWHGFEVYAEEMPDIR
                     RTLDELAEEVGRDPAELDLSATRGMVPPERVGETFIPDRRNLGESAAEMVDELGRYAE
                     AGVGLVLIQVGLLAPQTPGAMEWFAAEVMSQLG"
     gene            complement(1875..>2183)
                     /locus_tag="VEO00_08880"
     CDS             complement(1875..>2183)
                     /locus_tag="VEO00_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58148.1"
                     /translation="TLPPQPGQPSARHYLRQAVYLSTESVAIQPERLETNRGNGYPQP
                     LAFNNYQAATAGIFPNFDCDPSGGEKLEKTVLSAACVIAPPFGSEFGGGQAPNLYADP
                     "
BASE COUNT          308 a          788 c          739 g          348 t
ORIGIN      
        1 agctgtcggc gcccgcgtcc ctggcggcct cggcgcagcg cgcgacgacc tcggtgtcgg
       61 agtcctgcgg aacctcgtcg aacgtgcccg cgacctcgag cccgccgccc tcgatgccct
      121 cggccgcgcg tcccgggatc ccggtgtctc tgatcaccgc gtcggtcacg accatcaccc
      181 gggcggcccc ttcctttgcg aactcgaacc cggcgctctc gatcaggccc tcgccggcga
      241 tcacccgcgt cgggctggag aactggtaga agggcttgaa gcccgccatc tggcgggctc
      301 cggccattga ggcctcggct tccatcccgg cagtgcccgc gcgcgacgcc tagacgttga
      361 ccaggtcgtc gcggccctgc tgctctagct gggccttgta acgaggaaag accggcttgg
      421 tgagcggaac gagcttgagg atcttcgcga ccgggccctt ggccttgatc tgaccgcgcg
      481 ccatcgccac ggtgacgttg accttgccga gccagaagcg gtgcgccgtg tcggcctcca
      541 tgctcatcgt cacgtcgggc tccatctcag agtcgccgaa gtcgacctga cccggctctc
      601 cctcctgcag ccgcgtcgtg atcgccgagt cggggtcggt gaactcgtag cggacgatcg
      661 cgttcgcctt gttgaacttc ggcgccagct catcgtcctg ggataggtcc acgaacagct
      721 ttccgatcgt gtcgtagacg tcctgggcgt ccttgaaata ggccaaatcg tctctcctcc
      781 ctggggaaaa cggcattcga tgagcggggg gtgcgcccat ctgggccgaa tccttccaga
      841 atccccgcac agcggtcccc ggcggggacg cggcaacgcg gccgcccctg cgaatcgtca
      901 cccgagctgc gacatgacct cggcggcgaa ccactccatg gcccctggtg tctggggcgc
      961 gagcaagccg acctggatga ggacgagccc gacgcccgcc tccgcatagc gccccagctc
     1021 gtcgaccatc tcggccgccg attcgcccag gttgcggcgg tccggtatga aggtctcgcc
     1081 cacccgctcg ggcgggacca tgccgcgcgt cgcagacagg tccagctccg ccgggtcgcg
     1141 acccacctcc tcggccagct cgtcgagggt gcgccggatg tcgggcatct cctcggcgta
     1201 gacctcgaac ccgtgccacc cgtccccctg ccgcagggcg cggcgcagcg ccgggcggct
     1261 gttgcccccc acccagacgg ggggctcggc gccgcgtggg gtcatcgcga gcacgacgtc
     1321 gtcgaagtcg acgaagcggc cgtgaaacga ggccggatcg tcgcgccaca gcgtgcgcag
     1381 gacctcgatg tactcgtcaa gccgcgctcc gcgctccgcc gcgtcgaggc ccagggccgc
     1441 gaactcctcg cgcatccatc cggcccccac gccgaggatc agccggtcac cagacagcag
     1501 gtgtacggtc gcggcctccg ccgcgacgat gatcgggttg cggtagggca gcaccaggac
     1561 gctggtcccg agccgcacgc gctccgtgcg agaggccacg aacgccagcg tcgacagtgg
     1621 ctcgagccac cgtatccctg gcgtcatcgc catctccgtg ccggagcgcc cgtaggggta
     1681 ctcgctctgc cgcgcgtagg acatcagcgc gtggtcggtc gcccacaccg agtcgaagcc
     1741 gagccgctcc gccgtctccg cgacttccag cagctggtcg ggtttgtgat cgagcccgac
     1801 cgtcgggagc gcgacgccga tcttcacgcc gcgagtctaa gtgctgagtg gacccccgct
     1861 gaggtgcgcg cggcttacgg atccgcgtac aggttgggcg cctggccccc gccgaactcg
     1921 ctgccgaacg ggggcgcgat gacgcacgcg gcggagagga cggtcttctc gagcttctcg
     1981 ccaccgctgg ggtcgcagtc gaagttgggg aagatccccg ccgtcgcggc ctggtagttg
     2041 ttgaaggcca gcggctgcgg atagccgttg ccgcggttgg tctccagccg ttcgggctgg
     2101 atcgcgaccg actccgtgct caggtagacg gcctggcgca ggtagtggcg ggccgacggc
     2161 tggccgggtt gcggcggcag ggt
//
LOCUS       DASPWO010000386         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_396727, whole genome shotgun sequence.
ACCESSION   DASPWO010000386 DASPWO010000000
VERSION     DASPWO010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396727"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VEO00_08885"
     CDS             complement(<1..877)
                     /locus_tag="VEO00_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HYU58149.1"
                     /translation="MAVKFRDYYEVLGVPRTATAEEIKRAYRQLARKHHPDLQPASER
                     AKAAERFKEINEANEVLSDPEKRAKYDALGENWKGGMDFTPPPGAGGTWRTTDVPDWE
                     DAGGFSDFFSSLFGGTAGRSGRGNVRVVMPGSDVEAELPVTLEGLLHGGKRRITLDGR
                     SLDVEIPRGAREGTVLRLAGQGGAGIGGGPSGDVYLHLRLIPHPRYRAAGDDLELDLS
                     LSPWQAALGAEAKIETPDGTVTLKVPPGSQSGRRLRLRGRGLPRGDGSRGDLYAVVRI
                     VVPERLTTAEREAYEA"
     gene            complement(939..2840)
                     /gene="dnaK"
                     /locus_tag="VEO00_08890"
     CDS             complement(939..2840)
                     /gene="dnaK"
                     /locus_tag="VEO00_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HYU58150.1"
                     /translation="MGKVIGIDLGTTNSVVAVMEGGSPVVIPNQEGSRITPSVVAFTK
                     EGEILVGQVAKRQAITNPENTVFSIKRFMGRRHDEVQQEIKLVPYKVVKAPNGDARVE
                     IRGKQYSPPEISAMILRKLKEAAEAYLGQKVTQAVITVPAYFNDSQRQATKDAGKVAG
                     LEVQRIINEPTAAALAYGLDKKKDEQIAVYDLGGGTFDISILELGQGVFEVKATNGDT
                     HLGGDDFDQRVIDWIGEEFKKDHGVDLRRDRMALQRLKEAAEKAKIELSSTLQTEINL
                     PFITADASGPKHLVMTLTRAKLEALVDDLVQRTVPPCQQAMTDAAVTAGDVDEVVLVG
                     GQTRMPKVQELVKQVFGKEAHKGVNPDEVVAIGAAVQGGILAGDVKDVVLLDVTPLSL
                     GVETQGGAMTVLIPRNTTIPAKKSEIFTTAADTQTAVDIHVLQGERPLARDNRTLGRF
                     RLEGIPPAPRGMPQIEVAFDIDANGILNVSAKDNATGKQQQITITASSGLSKDEVDRM
                     VKDAEANAADDARRKQEIELRNQTDALVYQTERALAEHGPKLSESERSAVEGALNDAR
                     DALKSEDLERIRRAQENLTRISQTLAQAAYRDARGAGQAGGPQGPGGAPQGDVVDAEF
                     EDTDQRKAS"
     gene            complement(2896..3270)
                     /locus_tag="VEO00_08895"
     CDS             complement(2896..3270)
                     /locus_tag="VEO00_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYU58151.1"
                     /translation="MDKVGALVPWRAEHRDRPADEDALTLRDELVVTAEVPGLDREDL
                     ELTITPGALIIRGEKREEKQDQRKDFYMTEARYGRFVRTVPLPTGLDVDRASARVRHG
                     VLTVKFPKAAAGPGARRIAIKT"
BASE COUNT          413 a         1214 c         1122 g          607 t
ORIGIN      
        1 gcgcctcata cgcctctcgc tcagccgtgg tcagacgctc aggcaccacg atccggacga
       61 cggcgtagag gtcaccgcga ctgccatcgc cgcgcggcaa tccccggccg cgcagccgca
      121 accgccgtcc gctctgcgag cccggcggca ccttgagggt gaccgtgcca tcgggggtct
      181 cgatcttcgc ctcggcgccg agcgcggcct gccagggcga gagggacagg tccagctcca
      241 gatcgtcgcc ggctgcccgg tagcgcgggt gggggatcaa ccgcaggtgc agatagacgt
      301 ctcccgacgg tccaccaccg atgcctgccc cgccctgacc cgccaggcga agcacggtgc
      361 cctcacgcgc cccgcggggg atctccacgt cgagactccg gccgtccagc gtgatccggc
      421 gcttgcctcc atgcagcaac ccctcgagcg tcaccggcaa ctcggcctcg acgtcgcttc
      481 ccggcatcac cacgcggacg ttgccgcgtc cactccgccc cgccgtcccg ccgaagagcg
      541 acgagaagaa gtcgctgaag ccgccggcgt cctcccagtc cgggacgtcg gtcgtacgcc
      601 aggttccgcc ggcgccgggc ggcggcgtga agtccatccc gcccttccag ttctcgccga
      661 gcgcgtcgta cttggcgcgc ttctcgggat cgctcaggac ctcgttggcc tcgttgatct
      721 ccttgaaccg ctcggcagcc ttggcgcgct cggaggcggg ctggagatcg ggatggtgct
      781 tgcgcgcgag ctgccggtac gctcgcttga tctcctcggc ggtcgccgtg cggggcaccc
      841 ccagcacctc gtagtagtcg cggaacttca ccgccacgat cgggcagtct ccgcggtggt
      901 gaccaccacc tgcgccggat gcagcagctc gtcgccggtc aactggcctt ccgctgatcg
      961 gtgtcctcga attcggcgtc gacgacgtcg ccctgcggag cgccgcccgg gccctgcggc
     1021 ccgccggcct ggccagcgcc cctcgcgtct cggtacgcgg cctgggccag cgtctgcgag
     1081 atgcgcgtca ggttctcctg ggcccgccgg atgcgctcca ggtcctcgga tttcagcgcg
     1141 tccctggcgt cgttcagcgc cccctcgacc gcgctccgct cggactccga gagcttcggg
     1201 ccgtgctcgg cgagcgcgcg ctcggtctgg tacacgagcg cgtccgtctg gttgcgcagc
     1261 tcgatctcct gcttgcgcct cgcgtcgtcc gcggcgttgg cctcggcgtc cttcaccatc
     1321 cggtcgacct cgtccttgct gaggcccgac gaggccgtga tggtgatctg ctgctgcttg
     1381 ccggtggcgt tgtccttggc ggacacgttg aggatgccgt tggcgtcgat gtcgaacgcg
     1441 acctcgatct gcggcatccc gcgcggcgcc ggcggaatac cctcgaggcg gaaccgaccg
     1501 agcgtccggt tgtcccgcgc caggggccgc tccccctgga gtacgtggat gtccacggcc
     1561 gtctgggtgt ccgccgccgt ggtgaagatc tcgctcttct tcgcgggaat ggtcgtgttg
     1621 cgcgggatca acaccgtcat ggcgccgccc tgcgtctcga cgcccagtga gagcggcgtc
     1681 acgtcgagca agaccacgtc cttgacgtcg cccgcgagga tgcctccctg gacggccgcg
     1741 ccgatggcca cgacctcgtc ggggttgacg cccttgtggg cctccttgcc gaagacctgc
     1801 ttgaccagct cctggacctt cggcatccgg gtctgcccgc cgacgaggac gacctcgtcg
     1861 acgtcaccgg cggtgacggc cgcgtccgtc atcgcctgct ggcagggcgg gacggtgcgc
     1921 tgcacgaggt cgtccaccag cgcctcgagc ttcgcccgcg tcagggtcat caccaggtgc
     1981 ttggggccgc tcgcgtcggc ggtgatgaac ggcaggttga tctcggtctg cagcgtcgag
     2041 ctcagctcga tcttcgcctt ctccgcggcc tccttgagcc gctgcagcgc catgcggtcg
     2101 cggcgcaggt cgaccccgtg gtccttcttg aactcctcgc cgatccagtc gatcacccgc
     2161 tggtcgaaat cgtcgccgcc caggtgcgtg tcgccgttcg tcgccttcac ctcgaagacg
     2221 ccctggccga gctccaggat ggagatgtcg aacgtgccgc cgccgaggtc gtagacggcg
     2281 atctgctcgt ccttcttctt gtcgaggcca tacgccaggg cggcggcggt cggctcgttg
     2341 atgatgcgct gcacctcgag ccccgccacc ttgccggcat ccttcgtggc ctgacgctgg
     2401 ctgtcgttga agtacgcggg cacggtgatc acggcctgtg tgaccttctg gccgagatag
     2461 gcctccgcgg cctccttcag cttgcggagg atcatcgcgg agatctccgg tggggagtac
     2521 tgcttgcccc ggatctcgac gcgcgcgtcg ccgttgggcg ccttcacgac cttgtagggg
     2581 acgagcttga tctcctgctg gacctcgtcg tggcgacggc ccatgaaccg cttgatcgag
     2641 aagacggtgt tctccgggtt ggtgatcgcc tggcgcttcg cgacctggcc cacgaggatc
     2701 tcgccctcct tggtgaaggc gaccaccgac ggggtgatgc gacttccctc ctggttcggg
     2761 atgacgacgg ggctgccgcc ctccatcacc gcaacgaccg agttggtggt accgaggtcg
     2821 atgccgatca ccttgcccat cgggtcctcc tggtggagcc ccggtcgcgg tgggaaccgc
     2881 gaccggggcg gggggtcacg tcttgatcgc gatccggcgt gcgccgggtc cggcggcggc
     2941 cttcgggaac ttgaccgtca gcacgccgtg cctgacgcgt gccgaggcgc ggtccacgtc
     3001 cagcccggtc ggcaacggga cggtccggac gaagcgcccg taccgggcct cggtcatgta
     3061 gaagtccttg cgctggtcct gcttctcctc gcgcttctcg ccccggatga tcagcgcgcc
     3121 gggcgtgatc gtgagctcga ggtcctcacg gtcgagcccg ggaacctcgg cggtgaccac
     3181 gagctcgtca cggagcgtca gggcgtcctc atccgccggg cgatcgcggt gctcggcgcg
     3241 ccacggcacg agtgctccga ccttgtccat gatgcccatc gcagtcctcc gttcgctgat
     3301 gcttgtctcg tgccgtcaac tcgcgcccgg acgtgcggat cggctcgcct ctggca
//
LOCUS       DASPWO010000387         4076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_734809, whole genome shotgun sequence.
ACCESSION   DASPWO010000387 DASPWO010000000
VERSION     DASPWO010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734809"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VEO00_08900"
     CDS             complement(<1..315)
                     /locus_tag="VEO00_08900"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HYU58152.1"
                     /translation="MSRPRAARLAALLYGVAAAAYLIDRVTKVLAERTLEGRPPVDLI
                     PGVLRLRFTENPGGAFSLFVGAPSLFLVATLAATAAIVWASRSLSSAGVAAGLGLILG
                     GAL"
     gene            complement(312..719)
                     /locus_tag="VEO00_08905"
     CDS             complement(312..719)
                     /locus_tag="VEO00_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HYU58153.1"
                     /translation="MATTKTPRTPYTKKELAEFRARLEEERGQLVAQFEDIEESAFGD
                     PQSELSGEVSFDEEYADAGTATFERERDLSLVNNIRDLLEKVKKALERIDEGRFGLCE
                     RCGNPIEKARLKALPYASLCIRDKTLEERRRGR"
     gene            883..4041
                     /gene="ileS"
                     /locus_tag="VEO00_08910"
     CDS             883..4041
                     /gene="ileS"
                     /locus_tag="VEO00_08910"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HYU58154.1"
                     /translation="MGGRYRAIDPKEPMPDLEERVLGFWRDEDVIHRSFVWRRGAQEW
                     VFYDGPPTANNPPHIGHVEARTFKDLYPRFRTMAGFYVHRKAGWDCHGLPVEVEVEKM
                     IGTRSKRDIEAFGVARFIDLCRRSVERYVADWRRVSERMGHWIDMDDPYWTMTPEYVQ
                     SVWWAIKRLHELGLLFEDDKSSAYCPRCGTGLSDAEVALGYTTVTDPSVYVRFPVRDG
                     PQAVRGASFLAWTTTPWRLPSNVALAVHPELPYAVVRSGDERLLVAEAVVERLFGEDA
                     EVVTRVRGADLEGTTYEPPFQLFALDGPGHVVLTADFVAIDEGTGVVHIAPAFGQEDL
                     DLGKRHGLARVNPMDEEGRFDARAGRYAGMPVKEADPLLVEELGASGKLWRSEEYEHT
                     YPLCWRCETPLLYWARKGWYVRTSVRQQELLAANERVNWYPGHIKDGRYGDWLANNVD
                     WSLSRDRYWGTPLPIWRCPNGHVTVVGSLAELSPLAGRDLSSLDPHRPGIDGVTIACP
                     ECAESAARVPQVADAWFDSGAMPFAQWGYPHRGKEEFARRYPADFIAEGIDQTRGWFY
                     SLMAEGVLLFGENAYRNVICHGLVLDAEGRKMSKRLGNVIEPEDAFARFGADAVRWVM
                     VTSGSPWSDRRVSFDTIGDVVRRFLLTVRNTHAFFVTYANVDDVDGATLAVAVAERLP
                     LDRWILSQLQETVARTRGGLEAYDATGACRRLERFVDDLSNWYVRRSRRRFWDPAGET
                     AARDKEAAYATLYTCLTTLSRLLAPIVPFVAEDLYRGLVAEVDPQAPPSVHLTDYPAP
                     EEALRDPDLDDAMVIARQVASLGRAARTDAKIRVRQPLARALVHVPGDPARLAPLVPL
                     VADELNVREVRFAEQAAGELGRWRARPSFRALGPRLGPRVKDLAAALAADDGTLAARL
                     AAGEQVTVELGGEAVDLAPEDADLTRETPEGWDVASDGQVTVALDLVLTDDLRAEGLA
                     RELVRAVQDARREAGLEVTDRIRFVVGTSDPEVRAAFAAHEAFVRGEVLAVSLQVTEE
                     PPPGEAVVDIDGAAVGIALERA"
BASE COUNT          562 a         1421 c         1516 g          577 t
ORIGIN      
        1 cagcgctccg ccgaggatca acccgaggcc ggccgccacg cctgcggagg agagggagcg
       61 cgaggcccag acgatggcgg cggtggcggc gagcgtcgcc accaggaaca gcgagggcgc
      121 gccgacgaac agcgagaagg ccccgcccgg gttctcggtg aagcgcagcc gcaacacgcc
      181 ggggatcaga tcgaccggcg gccggccctc cagcgtgcgc tcagcgagga ccttggtgac
      241 gcggtcgatc aggtaggcgg cagcggcgac cccgtacagc agggcggcca gccgcgccgc
      301 gcgcggccgg ctcaccgacc ccgccggcgc tcctccaacg tcttgtccct gatgcacagg
      361 ctcgcgtagg gaagggcctt cagacgggcc ttctcgatcg ggttgccgca ccgctcgcag
      421 agcccgaacc tcccctcgtc gatccgctcc agcgccttct tcaccttctc cagcaggtcg
      481 cggatgttgt tcaccagcga gaggtcccgt tcgcgctcga aggtggccgt gccggcgtcg
      541 gcgtactcct cgtcgaagga gacctcgccg gacagctcgg actgcgggtc accgaacgcg
      601 gactcctcga tgtcctcgaa ctgggccacc agctgcccgc gctcctcctc caggcgagcg
      661 cggaactcgg ccagctcctt cttcgtgtag ggcgttcggg gggtcttcgt ggttgccatc
      721 gacggttcac ctgctcgggg ggggtcgctg aggtcgctgc gggccgaggg aacctagcac
      781 cccctgcccg cggttacaat cagggtacag gcggcgacgg gaacgagcgg gcagacgcct
      841 cgtccttcga gggacggggc gttgtcgtcc gaaaggagct ggatgggcgg acggtaccgg
      901 gcgatcgatc cgaaggagcc gatgccggac ctcgaggagc gggtgctcgg cttctggcgg
      961 gacgaggacg tcatccaccg ctcgttcgtg tggcgtcgcg gcgcccagga gtgggtcttc
     1021 tacgacggac ccccgaccgc gaacaacccg ccgcacatcg gccacgtcga ggcccgcacg
     1081 ttcaaggacc tctacccgcg cttccggacg atggccgggt tctacgtcca ccgcaaggcc
     1141 ggctgggact gccacggcct gccggtggag gtggaggtcg agaagatgat cggcacccgg
     1201 tcgaagcgcg acatcgaggc gttcggcgtc gcgaggttca tcgacctgtg ccgtcgatcg
     1261 gtggagcgct acgtcgcgga ctggagacgg gtcagcgagc ggatgggcca ctggatcgac
     1321 atggacgacc cgtactggac gatgaccccc gagtacgtcc agagcgtgtg gtgggcgatc
     1381 aagcggctgc acgagctggg cctgctgttc gaggacgaca agtcgagcgc gtactgcccg
     1441 cggtgcggca ccggcctgtc cgacgccgag gtcgccctgg gctacaccac cgtcaccgac
     1501 ccgagcgtct acgtccgctt cccggtgcgc gatgggccgc aggccgtccg cggcgcgtcg
     1561 ttcctggcct ggacgacgac gccctggagg ctgccgtcga acgtcgcgct ggctgtccac
     1621 cccgagctgc cctacgccgt ggtgcgctcc ggcgacgagc gcctgctggt ggccgaggcg
     1681 gtcgtcgagc gcctgttcgg ggaggacgcc gaggtcgtca cccgggtgcg cggcgccgac
     1741 ctcgagggga ccacctacga gccgccgttc caactattcg ccctggacgg ccccggccac
     1801 gtggtgctga ccgccgactt cgtcgccatc gacgagggga cgggcgtggt ccacatcgcg
     1861 ccggccttcg gccaggagga tctcgacctc gggaagcgcc acgggctggc gcgcgtgaac
     1921 cccatggacg aggagggcag gttcgacgcg cgagccggac gctacgccgg gatgccggtg
     1981 aaggaggccg acccgctcct cgtcgaggag ctcggcgcgt cgggcaagct ctggcgctcc
     2041 gaggagtacg agcacaccta tccgctctgc tggcggtgcg agacgccgct cctctactgg
     2101 gcgcggaagg gctggtacgt gcgaacgtcc gtccgccagc aggagctgct cgcggcgaac
     2161 gagcgggtga actggtatcc ggggcacatc aaggacggcc gctacggcga ctggctggcg
     2221 aacaacgtcg actggtcgct gtcgcgcgac cgctactggg gcacgccgct cccgatctgg
     2281 cgctgcccga acggccacgt caccgtcgtc ggctcgctgg ccgagctctc cccgctggcc
     2341 gggcgggatc tgtcgagcct ggacccgcac cgccccggga tcgacggggt gacgatcgcc
     2401 tgtcccgagt gcgccgagag cgccgctcgc gtgcctcagg tcgcggacgc ctggttcgac
     2461 tccggcgcga tgccgttcgc gcagtggggc tacccgcacc gcgggaagga ggagttcgcg
     2521 cggcgctatc cggccgactt catcgccgaa gggatcgacc agacgcgcgg ctggttctac
     2581 tcgctgatgg ccgagggcgt cctgctgttc ggcgagaacg cctatcgcaa cgtgatctgc
     2641 catggcctgg tgctcgatgc ggagggccgc aagatgtcga agcgtctcgg caacgtcatc
     2701 gagcccgagg acgcgttcgc ccgcttcggc gcggacgcgg tgcgctgggt gatggtcacc
     2761 tccgggtccc cgtggtccga ccgccgggtc tcgttcgaca cgatcggcga cgtcgtccgg
     2821 cggttcctgc tgacggtgcg caacacgcat gccttcttcg tcacgtacgc gaacgtcgac
     2881 gatgtcgacg gggccacgct ggccgtcgcc gtggccgagc gcttgccgct cgatcgctgg
     2941 atcctgtccc agttgcagga gaccgtggcc cggacgcggg gcgggctgga ggcatacgac
     3001 gccaccgggg cctgccggcg gctcgagcgg ttcgtggacg acctgtcgaa ctggtacgtg
     3061 cgccgctccc ggcgccggtt ctgggacccg gccggtgaga ccgcggcccg cgacaaggag
     3121 gccgcgtacg ccacgctgta cacgtgcctg acgacgctgt cgcggctgct ggcgccgatc
     3181 gtcccgttcg tggccgagga cctctaccgc gggctggtgg ccgaggtgga cccgcaggcg
     3241 ccgccgtcgg tgcacctcac cgactacccc gcccccgagg aggccctgcg ggacccggat
     3301 ctcgacgacg ccatggtgat cgcgcggcag gtcgcgtcgc tcgggcgggc tgcgcgcacc
     3361 gacgcgaaga tccgcgtccg gcagccgctc gcccgcgcgc tggtgcacgt gccgggcgac
     3421 ccggcgcgcc tcgcgccgct cgtcccgctc gtcgccgacg agctgaacgt acgagaggtg
     3481 cgcttcgccg agcaggcggc cggcgagctc ggccgctgga gggcgcggcc gagcttccgt
     3541 gcgctcggcc cacgcctcgg gccgcgggtg aaggacctcg ccgcggcgct cgccgccgac
     3601 gacgggacgc tcgccgcgcg cctggcggcc ggggagcagg tcacggtcga gctcggcggg
     3661 gaggccgtgg acctcgcccc cgaggacgcc gacctgaccc gcgagacgcc cgagggatgg
     3721 gacgtcgcct ccgacgggca ggtcaccgtg gcgctcgacc tggtgctgac cgacgacctg
     3781 cgggcggagg gactggcccg cgagctggtg cgggccgttc aggacgcgcg gcgcgaggcc
     3841 gggctggagg tcaccgaccg catccgtttc gtggtcggga cgagcgaccc ggaggtccgc
     3901 gcggcattcg cggcgcacga ggcgttcgtt cgcggcgagg tgctggcggt ttcgctgcag
     3961 gtgacggagg aacccccgcc cggcgaggca gtcgtggaca tcgacggggc cgcggtcggg
     4021 atcgcgctgg agcgtgcctg accggctacg gcttgcagac cccgcagggc gagtag
//
LOCUS       DASPWO010000388         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_137525, whole genome shotgun sequence.
ACCESSION   DASPWO010000388 DASPWO010000000
VERSION     DASPWO010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137525"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1186)
                     /gene="murB"
                     /locus_tag="VEO00_08915"
     CDS             complement(227..1186)
                     /gene="murB"
                     /locus_tag="VEO00_08915"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HYU58155.1"
                     /translation="MSDRRGRPVDAGAIARAESILRAACGDRVRTGFALAPLTTFRIG
                     GPAALFLEPQSMDDLEAAGHAVREAGIPFAVLGKGSNVLVADAGFPGLVVRLGKGFRW
                     AARDGTRLTAGGAMPLPALAGVALAHALAGLEFGVAIPASLGGSVRMNAGAHGHSLDE
                     VLEHVEVFEMASGAGRSIAAGDAGFRYRGSSLPEDAVVVAATVALRAGERGEIRRLME
                     EARDWRRATQPLAEPNCGSVFKNPPDGHAARMIEELGLKGAAVGGATVSSKHANFIVT
                     SPGARAADVVALIRLVQERVKETHGVTLEPEVHLVGEFDLANR"
     gene            complement(1183..>2128)
                     /locus_tag="VEO00_08920"
     CDS             complement(1183..>2128)
                     /locus_tag="VEO00_08920"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HYU58156.1"
                     /translation="NEVGSGARHGAGEWFVAESDESDGSFLLLHPEVAVVTNVGEDHL
                     DFYRDAREIRAAFARFFEQARHVVTCGDDPGVRAALRAAGREGDATTYGLHAGNDAVV
                     TLQDPGALGARGVLEVDGERIDLALRIRPQHNLLNAAAAILAAARAGVRPADAAEALR
                     SFQGVRRRFEFRGVGHGAEFFDDYAVHPTEVSAVLRAVSPEYRRVVAVLQPHRYTRMR
                     AMWRALGESLAPADVVVVTDVYGASEEPIPGISGKLVVEAIAEAEPRKRVVYLPRRSD
                     VAPFLAGEVRPGDLVLTLGAGDITMVADETLDRILGRA"
BASE COUNT          296 a          830 c          755 g          247 t
ORIGIN      
        1 gaggtccccg gtgtgaagcc acaggacgtt ggtgcccgga gccacgccgg caagacggag
       61 gacctgcgca cggtcgaagt gcgacgcgcc cgcgacctcg atggtgcggg cccggaacag
      121 cggcgaggtg gcgacgcccc aggcccccgc gccgaggacg gccacgccga gtggaacgga
      181 cagccaccgg gaaacccttc ggcgggccgg cgggccaccc ggtcgatcag cggttcgcga
      241 ggtcgaactc cccgacaagg tgcacctccg gctcgagggt gactccgtgc gtttccttca
      301 cccgttcctg gaccaggcgg atcagcgcga cgacgtcggc ggcgcgagcc cccggcgacg
      361 tgacgatgaa gttggcgtgc ttcgacgaca cggtggcccc gccgacggcc gcgcccttca
      421 gcccgagctc ctcgatcatc cgggcggcgt ggccgtcggg cgggttcttg aagacgctgc
      481 cgcagttcgg ctcggccagg ggctgggtgg cccggcgcca gtcccgggcc tcctccatga
      541 ggcggcggat ctcgccccgc tccccggccc ggagggcgac ggtcgcggcg acgacgacgg
      601 cgtcctcggg cagcgacgac ccccggtacc ggaaccccgc gtcgccggcg gcgatcgagc
      661 ggccggcgcc ggacgccatc tcgaacacct ccacgtgctc caggacctcg tcgagcgaat
      721 gcccgtgggc gccggcgttc atccgcaccg agccgccgag cgacgcgggg atggcgacgc
      781 cgaactccag gccggccagg gcgtgggcca gggcgacgcc cgcgagcgcc ggcaggggca
      841 tcgcgccgcc ggcggtcagc cgcgtcccgt cccgagccgc ccaccggaac cccttcccca
      901 gccgcacgac gagccccggg aacccggcgt cggccacgag cacgttcgat cccttcccga
      961 ggaccgcgaa cgggatgccg gcctcccgca cggcgtgccc ggccgcctcg aggtcgtcca
     1021 tggactgtgg ctccaggaac agcgccgccg gtccgccgat gcggaaggtg gtgagcggtg
     1081 cgagcgcgaa gccggtgcgg acgcggtcac cgcacgccgc gcggaggatg gactccgcgc
     1141 gcgcgatggc tccggcatcg accgggcggc ctcgtcggtc gctcatgctc tccccaggat
     1201 ccgatcgagg gtctcgtcgg ccaccatcgt gatgtcgccg gcaccgaggg tcagcaccag
     1261 gtcgcccggg cggacctccc ccgccaggaa cggcgccacg tccgagcgcc gcggcaggta
     1321 cacgacccgc ttgcgcggct cggcctccgc gatcgcctcc acgaccagct tcccgctgat
     1381 gcccgggatg ggctcctcgc tcgccccgta cacgtcggtc accacgacga cgtcggcggg
     1441 agccaggctc tcgccgaggg cccgccacat cgcccgcatc cgcgtgtacc ggtgcggctg
     1501 gagcaccgcg accacgcggc ggtactcggg agacacggcg cggagcaccg cggacacctc
     1561 cgtcgggtgc acggcgtagt cgtcgaagaa ctccgccccg tggcccacgc cccggaactc
     1621 gaaccgccgg cggactccct ggaacgatcg gagcgcctcc gcggcatcgg ccgggcggac
     1681 accggcgcgc gccgcggcga ggatggccgc ggcggcgttc agcaggttgt gctggggcct
     1741 gatccgaagc gccaggtcga tccgctcgcc gtcgacctcc agaacgccgc gggcgccgag
     1801 ggcgccggga tcctgcaatg tcaccaccgc gtcgttgccg gcatgcagcc cgtacgtcgt
     1861 ggcgtcgccc tcgcgccccg cggcccggag cgccgcgcgg acgccgggat cgtcgccgca
     1921 cgtcaccacg tggcgggcct gctcgaagaa gcgcgcgaac gcggcccgga tctcccgtgc
     1981 atcacggtag aagtccaggt ggtcctcccc cacgttcgtc accacggcga cctccgggtg
     2041 cagcaggagg aacgatccgt cgctctcatc cgactccgcg acgaaccact cccccgcacc
     2101 gtggcgcgcg cccgacccca cctcgttc
//
LOCUS       DASPWO010000389         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_567974, whole genome shotgun sequence.
ACCESSION   DASPWO010000389 DASPWO010000000
VERSION     DASPWO010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567974"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VEO00_08925"
     CDS             complement(<1..257)
                     /locus_tag="VEO00_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HYU58157.1"
                     /translation="MTTIAEGKRADLAGPGIGSYDEIERVLPRDYHSLLSPRETQEAI
                     FAAKRFIEDNLCRELNLTMVQVPLIVDAGFGVNDMLDRDASR"
     gene            470..>2229
                     /locus_tag="VEO00_08930"
     CDS             470..>2229
                     /locus_tag="VEO00_08930"
                     /inference="COORDINATES: protein motif:HMM:NF041501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGLLP motif-containing membrane protein"
                     /protein_id="HYU58158.1"
                     /translation="MGLGRGSRPSRLLAAGWLVLVAVGVLAGAALAQSPSPTGSPGLA
                     RPLAGLQDGDVFAAVGNGEVEWRAGDGTRKGTLSVGRSGQLTGMAFGPDGNLYVTNFD
                     ESLVHRLDLSGGSVPDFGSGYDAAPESIVFDADGNAYVGQAEGAADVLRFDRSGRLLR
                     RYDVRIEERGSDWIDLAGDQCTLVYTSEGSSIFRHDVCEDRRLTPFATGLPGTSAYAL
                     RLLPDGGLLVADNERILRLDGSGDIVTRYDAAGIDDWFALNLDPDGTSFWSASLSAPD
                     VFRFDIESGDVLARFAVETSSTDIGGLAVRGERTAARGGAWLDSVPTPGTLEKGLAHV
                     LATAGVAALIVLLMPFPAELFNKTLEEHYDELAARFAPARNRLARLRAARAERPEPVW
                     LAPAAIAVAALLYGFLNPSFGISLQSAALFGGLVLGLILAVVLFNLPFVLALGRAGVR
                     SRLRAYPGGLLVALGCVLASRLIGFQPGYLYGAIIGYGQDREVSAAREGRAVAVTVAW
                     KLAVSIAAWFARAPVHSAAVAGSPSFALVALDAALITVLVAGLEGATFGLLPLRFLDG
                     DALFRWNRAAWTALFGLGVFA"
BASE COUNT          233 a          802 c          841 g          353 t
ORIGIN      
        1 cgcgatgcgt cgcggtcgag catgtcgttc acgccgaacc cggcgtccac gatcagcggc
       61 acctggacca tggtcaggtt cagctcgcgg cacaggttgt cctcgatgaa ccgcttcgcc
      121 gcgaagatcg cctcctgcgt ctcgcgcggc gagagcagcg agtggtagtc gcgcggcagg
      181 acccgctcga tctcgtcgta gctcccgatc cccggaccgg cgaggtccgc ccgcttgccc
      241 tccgcgatcg tcgtcatcgc tctcatcccc ttcgtgcgct cgcacccacg tggcggcgcc
      301 ggccggatcg ggccggaggc catcccgatc ctgcggcggc cgccgccgca gcgggagtgc
      361 cgatcggccc gccggcggca tgccggtcgc cccgcagggc ccggggagcg gccccggcgg
      421 aagggttcgc ttgccccggc gccgtccccc gggtagcgtc gccgccgaga tgggtctcgg
      481 gcggggttcg cggccctccc ggctcctcgc ggccggctgg ctcgtcctcg tcgcggtggg
      541 ggtcctggcg ggagcggcgc tggcccagtc cccgtcgccc acgggctccc ccgggctggc
      601 caggccgctc gccggcctgc aggacgggga cgtcttcgcc gcggtcggga acggcgaggt
      661 cgagtggcgc gccggggacg gcacgcggaa ggggacgctc tcggtcggac ggtccggcca
      721 gctgaccggg atggcgttcg ggcccgacgg gaacctgtac gtcacgaact tcgacgagtc
      781 gctggtccac cggctcgacc tgtcgggcgg gtcggtcccc gatttcggct ccggctacga
      841 cgccgcgccg gagtcgatcg tgttcgacgc cgacggcaac gcgtacgtcg gtcaggctga
      901 gggcgccgcc gacgtcctga ggttcgatcg gtcggggcgg ctgttgcgcc ggtacgacgt
      961 gaggatcgag gagcggggca gcgactggat cgacctggcg ggcgaccagt gcaccctcgt
     1021 gtacacgtcg gaggggtcct cgatcttccg gcacgacgtc tgcgaggacc gccgtctgac
     1081 gccgttcgcc accggtctcc cggggacgtc cgcctacgcg ctgcgcctcc tcccggacgg
     1141 cggactgctg gtggccgaca acgagcgcat cctgcgcctc gacgggagcg gcgacatcgt
     1201 cacgcgctat gacgccgcgg ggatcgatga ctggttcgcg ctgaacctgg accccgacgg
     1261 gacgtcgttc tggagcgcgt cgctcagcgc gccggacgtc ttccggttcg acatcgagtc
     1321 cggcgacgtg ctcgcgcggt tcgcggtcga aacgtcgtcc acggacatcg gtgggctggc
     1381 cgtgcggggt gagcgcaccg ccgcgcgggg aggcgcctgg ctggactcgg tgccgacgcc
     1441 ggggacgctg gagaaggggc tggcccacgt actggcgacg gcgggggttg ccgccctcat
     1501 cgtcctcctg atgccgttcc cggcggaact cttcaacaag acgctcgagg agcactacga
     1561 cgagctggcg gcgcggttcg cgccggcccg gaaccggctg gcccgcctgc gcgcggcccg
     1621 tgccgagcgc cccgagcccg tctggctggc cccggcggcg atcgcggtgg cggccctgct
     1681 ctacgggttc ctcaacccgt cgttcgggat cagcctgcag tcggcggcgc tgttcggcgg
     1741 cctggtcctc gggctgatcc tggccgtcgt gctgttcaac cttccgttcg tcctggcgct
     1801 ggggcgcgcc ggcgtgcggt cccggctccg cgcctacccc ggggggttgc tcgtggcgct
     1861 cggctgcgtg ctggcctcgc gcctgatcgg gttccagccg ggctacctgt acggcgcgat
     1921 catcgggtac ggacaggacc gcgaagtctc cgcggcccgc gagggacgcg ccgtcgccgt
     1981 gaccgtggcg tggaagctgg cggtcagcat cgcggcgtgg ttcgcgcggg cgccggtgca
     2041 ctcggcggcg gtggctggca gcccgtcgtt cgcgctggtc gccctcgacg ccgcgctgat
     2101 caccgtcctc gtcgccggcc tggagggcgc cacgttcggc ctgcttccgt tgcgcttcct
     2161 cgacggggac gcgctgttcc gctggaaccg ggctgcctgg acggcgctgt tcgggctcgg
     2221 cgtgttcgc
//
LOCUS       DASPWO010000390         4828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_226288, whole genome shotgun sequence.
ACCESSION   DASPWO010000390 DASPWO010000000
VERSION     DASPWO010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226288"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VEO00_08935"
     CDS             <1..207
                     /locus_tag="VEO00_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HYU58159.1"
                     /translation="DLPERIEELPEVEDCYSVAGAENYVLKVRTRTPADLEDFLRRLR
                     EKAGVQTRTTVVLSIPFEHRPVRP"
     gene            complement(277..1446)
                     /locus_tag="VEO00_08940"
     CDS             complement(277..1446)
                     /locus_tag="VEO00_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU58160.1"
                     /translation="MLAVDRDFGLDEEQRELVKVVRAFARDRAGPAAPAHEEAKTFPR
                     ELFRELGAMGLGGIPYDPEYGGGGQPFLTYLLVLEELATAYLALAVGLSVHHLSAFGL
                     HEFGSEPLKTRYLPRLFSSEWLGAYALSEASAGSDAAALECRAHRSDGGYRLTGGKRF
                     ITHGGEAEYYLVMARTGEEGAAGISAFVVEKGWEGFSFGAPEAKMGWHSSPMRELIFQ
                     ACPVPEENRIGEEGQGFRIALAALVSGRLGIAAVSTGLAEGALDAAVRFARERTQFGR
                     PVLDFQGIQFMLADMLVQVEASRRLYRDAARASAAGEDATLAASIAKLAASDTAMRVT
                     TDAVQIHGGYGYMREYPVERYMREAKALQIVEGTNQVQRMIIGRRLADPTWRPAV"
     gene            1512..2903
                     /gene="cysS"
                     /locus_tag="VEO00_08945"
     CDS             1512..2903
                     /gene="cysS"
                     /locus_tag="VEO00_08945"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HYU58161.1"
                     /translation="MRLNNTLTNRVEDLVPVEEGRVRIYGCGPTVYRYVHVGNLRTFL
                     LSDVLRRALRLEGYDVTLVMNITDVGHMTDEVSDAGRDRMELSVEDEGLSPAEIAQKY
                     TQAYLEDTGAIGIEPADVMPKATDHIPEMLELTAGLLERGHAYEVNGTVYFDVHSFPG
                     YGKLSGNTLDKLRTGHREIEVDPNKRHPEDFALWKRAGANRLMRWDSSWGPGFPGWHI
                     ECSAMSMKYLGERFDIHTGGNDLKFPHHEDEIAQSEGVVGHQVVSIWVHTGFLNMSGV
                     KMSKSRKNVVRIGDLVEELGIDPLAFRYLCFQTRYRSEMDLSLEALKAANHTLRRLRA
                     RMADWAGATRDGLGAAARDLDGRFRAAVADDLDLPAAVTIVSEAVSADVTDGDRYALL
                     ASWDGVLGLDLEREAREGVEVPEEVRALVRERDDARARKDFARSDALREEIRALGFEV
                     RDGAGGTEVRPGT"
     gene            2955..>4828
                     /locus_tag="VEO00_08950"
     CDS             2955..>4828
                     /locus_tag="VEO00_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYU58162.1"
                     /translation="MSSVPLSPRSRVGLLATAVLLVGLALAGRPGPARSAAEAPLRPN
                     IVLIVSDDQRWDTVGKCLGGFDGADLDAGASACMPSLQSQLMANGTTFPAAHVTTSLC
                     CPSRSSILTGRYARYTGVIDNFSGGELDVTNTLNVWLDAAGYRTGLVGKWLNEYGQPD
                     GPWPQDYLPPGWDWWTAFWGDLDFRDYRLAESRDGGPVTAQEHVWDPTDPTDACVEGN
                     RHADDLLCARVLQFLAQPSTDPFFLLLASHAPHPPFTPPVRWDGWADGIERPEYPSLN
                     AVPTPNTPGYLASRQPYTADQLARFEHQFRDALESTRAIDDGIGAIRAGLAADGRLPN
                     TVFVYVSDNGYAFGEHRSSGKDCIYEECHRVPIVIDCPPAVCPDAVPGRVVSSPLAAL
                     NIDLAPTISELAGIAPGGPVDGRSLVPWLRSDATDWRTHWLFEDRALERSGVVALLGD
                     EHLYKYAQIETAPGEELYDLTADPWELTNLSKNSAYADELSQLRAIFASYDEPPQPNA
                     PPTASITAPAPDALLSAKKVSVSWTASDDVAVLRTDLYERIPPSGTATRVQSGTGTSF
                     VRTGTLGRTYCYAVTAVDGEGATGSSGEHCAAVPYDDRATQFTRSGTLDALSATGAYQ
                     G"
BASE COUNT          715 a         1772 c         1683 g          658 t
ORIGIN      
        1 gacctgccgg agcggatcga ggagctgccc gaggtcgagg actgctactc ggtggccggc
       61 gccgagaact acgtgctgaa ggtgcgcacc cgcaccccgg ccgacctgga ggacttcctg
      121 cgccgcctgc gcgagaaggc cggcgtgcag acccgcacca ccgtggtgct ctcgatcccc
      181 ttcgagcacc gcccggtccg gccctgacgc cgcgcgtctt tcagcgcggc ttggagtcgc
      241 cgaccgccac cgccacacgg ccggacgtga tccggttcac acggcgggtc gccaggtggg
      301 atctgccagg cggcggccga tgatcatccg ctggacctgg ttggtcccct cgacgatctg
      361 cagcgccttc gcctcgcgca tgtagcgctc gaccgggtac tcgcgcatgt acccgtagcc
      421 gccgtggatc tgcacggcgt cggtggtcac ccgcatcgcc gtgtcggagg cggcgagctt
      481 cgcgatggag gccgccaggg tggcgtcctc cccggcggcg ctggcccggg cggcgtcgcg
      541 gtacaggcgc cgggaggcct ccacctgcac cagcatgtcg gcgagcatga actggatgcc
      601 ctggaagtcg aggaccggcc gcccgaactg cgtgcgttcg cgggcgaagc gcacggcggc
      661 gtcgagggcc ccctcggcca gccccgtgga gacggcggcg atccccagac gcccgctcac
      721 gagagcggcc agcgcgatcc ggaacccctg gccctcctcc ccgatccggt tctcctccgg
      781 gaccgggcag gcctggaaga tcagctcgcg catcggcgaa gagtgccagc ccatcttggc
      841 ctcgggcgcg ccgaacgaga agccctccca ccctttctcc accacgaacg ccgagatccc
      901 ggccgccccc tcctccccgg tgcgggccat cacgaggtag tactcggcct cgcccccgtg
      961 ggtgatgaag cgcttgccgc cggtcagccg gtacccgccg tcggagcggt gcgcgcgaca
     1021 ctccagcgcc gcggcgtccg agccggctga tgcctccgac agcgcgtagg ccccgagcca
     1081 ctccgaggag aacaggcgtg gcaagtagcg cgtcttcagt ggctcggagc cgaactcgtg
     1141 taggccgaag gccgacaggt ggtgcaccga cagcccgaca gcgagcgcca ggtaggccgt
     1201 ggccagctcc tcgaggacca acagataggt caggaagggc tggccgccgc cgccgtactc
     1261 ggggtcgtac gggatcccgc cgaggcccat cgctccgagc tcccggaaca gctcccgcgg
     1321 gaacgtcttc gcttcctcgt gcgccggcgc cgccggcccg gcgcggtcgc gggcgaacgc
     1381 ccggaccacc ttgaccagct ctcgctgctc ctcgtcgagc ccgaagtccc ggtccaccgc
     1441 gagcatcgta gccgagcggg gcgtggccgc cgccggcaga gcgcccggcg accgccgcta
     1501 tgctctctcc gatgcgcctg aacaacacgc tgacgaaccg cgtcgaggat ctagtcccgg
     1561 tcgaagaggg acgggtcagg atctacggct gcgggccgac cgtctaccgc tacgtccacg
     1621 tcggcaacct acgaacgttc ctgctcagcg atgtgctgcg gcgggcgctg cgactggagg
     1681 ggtacgacgt cacgctcgtc atgaacatca ccgacgtcgg gcacatgacc gacgaggtga
     1741 gcgacgccgg gcgcgaccgg atggagctgt ccgtcgagga cgaggggctc tcccccgccg
     1801 agatcgcgca gaagtacacg caggcctacc tcgaggacac gggggcgatc ggaatcgagc
     1861 cggccgacgt gatgccgaag gcgacggacc acatccccga gatgctcgag ctgaccgccg
     1921 gactgctcga gcgtgggcac gcctacgagg tgaacgggac ggtctatttc gacgtgcact
     1981 cgttccccgg gtacggcaag ctctccggga acacgctgga caagctgcgc accggccacc
     2041 gcgagatcga ggtcgacccg aacaagcgcc accccgagga cttcgcgctg tggaagcgcg
     2101 ccggggcgaa ccggctgatg cgctgggaca gctcctgggg cccggggttc cccggctggc
     2161 acatcgagtg ctcggcgatg tcgatgaagt acctcgggga gcgcttcgac atccacaccg
     2221 ggggcaacga cctgaagttc ccccaccacg aggacgagat cgcccagtcg gagggcgtgg
     2281 tcggccacca ggtcgtcagc atctgggtgc acacggggtt cctgaacatg tccggcgtga
     2341 agatgtcgaa gtcccgcaag aacgtcgtcc ggatcggcga cctggtcgag gagctcggga
     2401 tcgacccgct ggcgttccgc tacctctgct tccagacccg gtaccgcagc gagatggacc
     2461 tctccctcga ggcgctgaag gcggccaacc acacgctccg ccggctgcgg gcgcggatgg
     2521 cggactgggc cggcgcgacc cgcgacggac tcggcgccgc ggcgcgggac ctggacggcc
     2581 ggttccgggc ggccgtcgcc gacgacctgg acctccccgc cgcggtgacg atcgtctccg
     2641 aggccgtctc ggccgacgtc accgacggcg accgctacgc cctgctggcc agctgggacg
     2701 gcgtgcttgg gctcgacttg gagcgcgagg cgcgcgaggg cgtcgaggtg cccgaggagg
     2761 tgcgcgcgct ggtccgcgag cgcgacgacg cccgcgcccg gaaggacttc gcccgttccg
     2821 acgcgctccg cgaggagatc cgcgcgctcg ggttcgaggt acgcgacggc gccggcggga
     2881 ccgaggtccg ccccggcacc tagagcgcga ccctggacgg aggttcggcc ggggcctacg
     2941 ctgtgccggt tcccgtgtcc tccgtcccgc tctcgccgcg ttcccgcgtt gggctcctcg
     3001 ccacggcggt gctgctcgtc ggcctggcgc tcgcaggccg gcccggcccg gcccgttcgg
     3061 cggctgaggc acctcttcgg ccgaacatcg tgctgatcgt cagcgacgac cagcgctggg
     3121 acacggtcgg gaagtgcctg ggcggcttcg acggggcgga cctggacgcc ggcgcgagcg
     3181 cctgcatgcc gagcctgcag tcacagctga tggcgaacgg cactacgttc cccgccgccc
     3241 acgtcacgac gtcgctgtgc tgccccagcc gctcgagcat cctgacaggg cgctacgccc
     3301 ggtacacggg cgtgatcgac aacttctccg gcggcgagct cgacgtgacg aacacgctga
     3361 acgtgtggct ggatgcggcg ggctaccgca ccggcctggt cggcaagtgg ctgaacgagt
     3421 acggccagcc cgacggcccg tggccgcagg actacctgcc tccgggctgg gactggtgga
     3481 cggccttctg gggggacctc gacttccgcg actaccggct cgcggagtcc cgtgacggcg
     3541 gaccggtcac ggcgcaggag cacgtctggg acccgaccga tcccaccgac gcctgcgtcg
     3601 aggggaaccg ccacgccgac gacctgctgt gcgcccgcgt gctgcagttc ctggcgcagc
     3661 cgtcgaccga cccgttcttc ctgctcttag cctcccacgc cccgcacccg cccttcacgc
     3721 caccggtgcg ctgggacggc tgggccgacg ggatcgagcg gccggagtac ccgtccctga
     3781 acgcggttcc taccccgaac acgcccggct acctcgcctc gcggcagccc tacacggcgg
     3841 accagctcgc caggttcgag caccagttcc gcgacgccct ggagtccacc cgcgcgatcg
     3901 acgacggcat cggcgcgatc cgcgccgggc tggccgccga cggacggctg ccgaacacgg
     3961 tgttcgtgta cgtctccgac aacggctacg cgttcggcga gcaccgcagc tccggcaagg
     4021 actgcatcta cgaggaatgc caccgggtcc cgatcgtgat cgactgtccg ccggcggtgt
     4081 gcccggatgc cgtccctgga cgcgtggtga gcagcccgct ggccgccctg aacatcgacc
     4141 tggcgccgac catctccgag ctcgcgggga tcgcgccggg cgggccggtg gacgggcgca
     4201 gcctggtgcc gtggttgcga tccgacgcca cggattggcg gacgcactgg ctgttcgagg
     4261 accgcgcgct cgagcgcagc ggcgtcgtcg ccctgctcgg ggacgagcac ctctacaagt
     4321 acgcgcagat cgaaaccgcc cccggcgagg agctatacga cctgacggcc gatccctggg
     4381 agctgacgaa cctgtcgaag aactcggcct acgccgacga gctctcgcag ctgcgggcca
     4441 tcttcgcctc ctacgacgag ccgcctcagc cgaacgcgcc gccgacggcg tcgatcaccg
     4501 cgccggcgcc cgacgccctc ctctcggcga agaaggtgtc ggtctcctgg accgcctccg
     4561 acgacgtggc ggtcttgcgc accgacctgt acgagcggat cccgccgtcg ggcacggcca
     4621 cccgcgtcca gtccgggacg ggcacctcgt tcgtccgcac cggcacgctc ggcaggacgt
     4681 actgctacgc ggtgacggcg gtggacggtg aaggggcgac cggaagctcc ggtgagcact
     4741 gcgcggccgt cccgtacgac gaccgcgcga cgcagttcac acgctcgggg acgctcgacg
     4801 cgctctcggc gacaggcgcg taccaggg
//
LOCUS       DASPWO010000391         7725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_905653, whole genome shotgun sequence.
ACCESSION   DASPWO010000391 DASPWO010000000
VERSION     DASPWO010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905653"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VEO00_08955"
     CDS             <1..277
                     /locus_tag="VEO00_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58163.1"
                     /translation="NVGLPIVECPGVSGDLRTGDLVRVDFGRGVVLDVAQGRSHEATP
                     LPPFMQEILEFGGLMRWVRRSLDAGGRVEVEVGVSDQEPVQADWRLD"
     gene            274..1068
                     /locus_tag="VEO00_08960"
     CDS             274..1068
                     /locus_tag="VEO00_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HYU58164.1"
                     /translation="MTPRTHRVAVIPGDGTGPEVVREGLKVLAAVSDGFAVERTVFDL
                     GGERYLRTGETLPHAALAELRGHDAVYLGAIGHPDVAPGVLERELLLRIRVALDQYVN
                     LRPVRLYPGVRSPIAGLEPGDVDLVFVRENTEGLYVGRGSFTARDTAGEVAVQESVNT
                     RRGVERCLRYAFGLAGRRRRHVTMVHKTNVLNFGGDLWSRAFAAVAAEHPTIETAYVH
                     VDAACVYLVEDPGRFDVVVTESCGGRSPRRRPVRTDGAGRRHPDLP"
     gene            1037..1381
                     /locus_tag="VEO00_08965"
     CDS             1037..1381
                     /locus_tag="VEO00_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HYU58165.1"
                     /translation="MLADGIQTYPENHTKFGVVGQPTARFGRPDKTSIVFAGNDVPGS
                     LLRCLQPFADRRINLLKLESRPQGARPWNYVFYLDLEAAADDPPAAEALSELSEHTSF
                     VRVLGTYPAWRD"
     gene            1453..3048
                     /locus_tag="VEO00_08970"
     CDS             1453..3048
                     /locus_tag="VEO00_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYU58166.1"
                     /translation="MPRRRSLYRSRSHSASGRGFAAFVLLMVLLTGGVIVWRTGLLGF
                     GGGGQASATGPTGRPPGPFDSERPTESPTPSPSPSPTIEGGGPINPDFPNLTTFRGNA
                     TRTYYGEGPFPKNPTVRWTYPSSGGLCSVSDGGSGPKTWCGTGWTGQPNVIEKPNGSV
                     EIRIGAYDAHYHFLAGRSGEPVRPDLVTGDLAKGSATSDPDGYPLYYAGSRDNQLRVV
                     ALDRPEPTELWSIDANTSVPDPTWNDDWDGAPLIIGDYLLEGGENSWFYVIKLNRGYD
                     GAGKVTVDPTVVLTVPGYDDQLIQDLGDTDISIESSVAFFDGVAYFSNSGGLVQGWDL
                     HDILRGGTEATRVFRYWVGDDVDATIAIDQQGYLYVARHKEHNVTSRVNPFAGSLGNL
                     VKLDPRKRQKPVVWSAWIGSSSGGGDILGTPALYEGYVYIGSVDGELTAVNMKNGRKA
                     WSETLPGPLNASPVVIDDRLLMGDCLGGLHLYDVSDPTASPRKLWTLQLEGCVESTPA
                     VWKGWIWVGTRAGPIYGIADPGA"
     gene            3053..3871
                     /locus_tag="VEO00_08975"
     CDS             3053..3871
                     /locus_tag="VEO00_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HYU58167.1"
                     /translation="MPPTGLSFRPRTRVRYGRILVVLAALGGLVAFVWLGPFRGDGSP
                     AVDASPSAGGASDRPPMCAQADAPARLAAVGDWEHTLVDTRFTLPAAYVPPGLVSTTE
                     AGFDGAFLVRDFVVPDLIALRRAAEAAGNPVEVIASYRSYDDQAELFQRQVDRFGEEV
                     ALQRTARPGHSEHQLGTTVDFKTRGEDDVSPYWEDTPAGAWMAGHAVQYGFVMSYPRG
                     KDAVTCYAYEPWHFRYFGRGRAAKIAASGLTVREFLWREQRPATRATPSPSASP"
     gene            3963..4253
                     /locus_tag="VEO00_08980"
     CDS             3963..4253
                     /locus_tag="VEO00_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017555658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYU58168.1"
                     /translation="MVGDVYRALADPTRRTILDELTERDGQTLFEICTRLTMKHGLSS
                     SRQAISQHLEVLEAAGLVRTKREGRYKFHFIETTPLRAVVERWPIDRGEDHR"
     gene            4250..4648
                     /locus_tag="VEO00_08985"
     CDS             4250..4648
                     /locus_tag="VEO00_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003957969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU58169.1"
                     /translation="MRINVTGVYVDDQHKALRFYTEVLGFVKKTEIPLGEASWLTVVS
                     PDEPHGTELILEPDAHPAVRPFKEALVDDGIPFTSFAVDDVRTECERLRSLGVRFTQE
                     PLAMGPVTTAVFDDTCGNLIQIATAREATG"
     gene            complement(4645..4971)
                     /locus_tag="VEO00_08990"
     CDS             complement(4645..4971)
                     /locus_tag="VEO00_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYU58170.1"
                     /translation="MSGRDERVAAVFQALSDPTRRAVVRALSEHGEGATATELAGEFL
                     VTRQAVSKHLAALAEAGLVESERIGRAVRYRLTPRPMAEAMGWMAGVGAEWDERLAAL
                     RRHLRG"
     gene            complement(4968..5321)
                     /locus_tag="VEO00_08995"
     CDS             complement(4968..5321)
                     /locus_tag="VEO00_08995"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYU58171.1"
                     /translation="MEREIVLPVPPGEVWRALTDPGRLGAWFGANVELDPRPGGHGRF
                     RFPDGSERAMMVEESDRPRRFAFRWAPFRRRPDGSAEPTEPTRVAFELEEDHGGTRLT
                     VTETVVEASPAGARP"
     gene            5424..6074
                     /locus_tag="VEO00_09000"
     CDS             5424..6074
                     /locus_tag="VEO00_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HYU58172.1"
                     /translation="MSQGLVPYVVEETARGERSFDIYSRLLKERVVFLGREVDDDLAN
                     LLTAQLLHLEAEDPERDINLYINSPGGSATALFAIYDTMQFVKPDVATTCVGQAASAA
                     AVLLAAGALGKRACLPNARVLIHQPWGQVGGQAVDMEIHAREAIRHRRLMDEILARHT
                     GQPVEKVSHDTDRDFILTAEQAKDYGLVDMILARRVGPLAAFEPDETAPENGDRAG"
     gene            6103..6552
                     /locus_tag="VEO00_09005"
     CDS             6103..6552
                     /locus_tag="VEO00_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYU58173.1"
                     /translation="MAPIQPGTKAPPAPGVAFDGPTALIFYKVTCPVCQLAGPPLDRL
                     RNAFPDAVIAVGQDPPEKLEAFAREWRFRVPAVSDPPPYPISDAYGIRTVPTVFLVDR
                     DGTVLETVESWDREGFNRVSRRLAELTGTEYAEVSHPSDGLPAFRPG"
     gene            6631..7101
                     /locus_tag="VEO00_09010"
     CDS             6631..7101
                     /locus_tag="VEO00_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU58174.1"
                     /translation="MLVDSRPQDELERLYRDQRDRMWQAVFAFAGDRDVASDAVAEAF
                     AQALRRGDAVRSPERWIWRTIFRIAAGDLKERRRHQSHPVERTYEMEELARDLVAALG
                     KLPPRQRAAVVLHHAVGYPAAEVARILGSTTAAIHVQISRGRKRLRELLETDDA"
     gene            7376..>7725
                     /locus_tag="VEO00_09015"
     CDS             7376..>7725
                     /locus_tag="VEO00_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58175.1"
                     /translation="MDGVFAAMLDAIRSSSPPGWLFALQPDRLDGDWRMDGAVDDGSG
                     PGRLFVVVTIRPGMIQAHPCTDMEFRQRGRCVERTLPGGDRLVLRDVVVEAGGMKTIV
                     AVLVHPDGSGVSAEA"
BASE COUNT         1088 a         2767 c         2754 g         1116 t
ORIGIN      
        1 caacgtcggc ctgccgatcg tggagtgtcc gggggtctcg ggcgacctcc gaacggggga
       61 cctcgttcgc gtagacttcg gccgcggggt ggttctcgac gtggctcagg ggcgcagcca
      121 cgaggccacc ccgcttcctc ctttcatgca ggagatcctg gagttcggcg ggctgatgcg
      181 ctgggtccgc cgctcgctcg acgcgggcgg ccgggttgag gttgaggttg gggtctcgga
      241 ccaggagccg gtccaggcgg attggcggct ggattgaccc cgcgcacgca ccgcgtggcg
      301 gtcatcccgg gggacgggac cggcccggag gtggtccgcg agggcctgaa ggtcctggcg
      361 gcggtgtccg acgggttcgc cgtcgagagg accgtcttcg acctcggggg cgagcggtac
      421 ctgcgtaccg gggagaccct cccgcacgcc gccctggccg agctgcgggg ccacgacgcg
      481 gtctacctcg gcgcgatcgg gcaccccgac gtggcgccgg gggtcctgga gcgcgagctc
      541 ctcctgcgga tccgcgtcgc gctcgaccag tacgtcaacc tccggccggt gcggctctac
      601 cccggggtcc gatcgccgat agccggcctg gagccgggcg acgtggacct ggtcttcgtc
      661 cgcgagaaca ccgaaggcct ctacgtcggg cgcggctcgt tcacggcccg cgacacggcc
      721 ggcgaggtcg ccgtgcagga gagcgtgaac actcgccgcg gggtggagcg gtgcctgcgc
      781 tacgcgttcg gcctcgccgg acgtcgccgc cggcacgtga cgatggtgca caagacgaac
      841 gtcctgaact tcggggggga cctgtggagc cgggccttcg ccgccgtcgc cgcggagcac
      901 ccgaccatcg agaccgccta cgtccacgtg gacgcggcct gcgtctacct ggtcgaggac
      961 cccgggcggt tcgacgtggt ggtgacggag agctgcggtg gccggtcgcc gcgccgccga
     1021 cctgtacgga ctgacggtgc tggccgacgg catccagacc taccctgaga accacacgaa
     1081 gttcggcgtg gtcggccagc ccaccgctcg tttcggacgg cccgacaaga cctcgatcgt
     1141 gttcgccggg aacgacgtgc ccggctcgct gctgcgctgc ctgcagccgt tcgcagaccg
     1201 ccgcatcaac ctgctgaagc tcgagtcgcg cccgcaagga gcgcgcccct ggaactacgt
     1261 cttctacctg gacctcgagg cggccgcgga cgacccgccc gccgccgagg cgctgtcgga
     1321 gctctccgag cacacgtcgt tcgtccgcgt cctcgggacg taccccgcgt ggcgggactg
     1381 agcccggagg tccgtgccga gcgccgggga ggaccgtaga ctctctccgc aatccgacct
     1441 ccccccaacc acgtgcccag gcgccggtct ctctaccgct cccgttcgca ctcggcctcg
     1501 ggccggggct ttgcggcgtt cgtcctgctg atggtgctct tgaccggcgg ggtgatcgtc
     1561 tggcgcaccg gcctgctcgg gttcggcggc ggggggcaag cctctgcgac cgggccgacc
     1621 ggcaggccgc cggggccgtt cgactccgag cggccgacgg agtccccgac cccgtccccc
     1681 tcaccgtctc ccaccatcga gggcggcggg ccgatcaacc ccgacttccc gaacctgacg
     1741 accttccgcg gcaacgccac ccgcacgtac tacggcgagg gcccgttccc gaagaacccc
     1801 acggtccgct ggacgtaccc gtcctcgggc gggctgtgca gcgtctccga cggtgggtcc
     1861 gggccgaaga cctggtgcgg cacgggatgg accgggcagc cgaacgtcat cgagaagccg
     1921 aacgggagcg tcgagatccg gatcggcgcg tacgacgcgc actaccactt cctggccggg
     1981 cggagcggcg agccggtgcg gcccgacctg gtcaccggcg acctggccaa gggctccgcg
     2041 acctcggacc cggacgggta tcctctctac tacgcgggct cgcgcgacaa tcagctgcgc
     2101 gtcgtggccc tcgaccggcc cgagccgacc gagctctggt cgatcgacgc caacaccagc
     2161 gtccccgacc ccacctggaa cgacgactgg gacggcgcgc cactgatcat cggcgactac
     2221 ctgctcgagg gcggcgagaa ctcgtggttc tacgtgatca agctgaaccg cggctacgac
     2281 ggagccggca aggtgacggt cgaccccacg gtggtgctca cggttccggg ctacgacgac
     2341 cagctgatcc aggacctcgg ggacaccgac atctcgatcg agtcctcggt cgcgttcttc
     2401 gacggcgtgg cctacttctc gaactcgggc ggtctggtcc agggctggga cctgcacgac
     2461 atcctgcggg gagggaccga ggcgacgcgc gtcttccgct actgggtggg tgacgacgtc
     2521 gacgccacca tcgccatcga ccagcagggg tacctgtacg tggcccggca caaggagcac
     2581 aacgtcacca gccgcgtgaa cccgttcgcc ggcagcctcg ggaacctggt caagctcgac
     2641 ccccgcaagc ggcagaagcc cgtggtgtgg tcggcgtgga tcgggtcgtc ctccggcggg
     2701 ggggacatcc tcggcacgcc ggcgctgtac gagggctacg tctacatcgg ctcggtggac
     2761 ggcgagctga ccgcggtgaa catgaagaac ggcaggaagg cgtggtccga gacgctgccg
     2821 ggcccgctga acgcctcgcc cgtcgtgatc gacgatcggc tactgatggg ggactgcctg
     2881 ggcgggctgc acctctacga cgtctccgac cctacggcct cgccccggaa gctgtggacg
     2941 ctgcagctcg aagggtgcgt cgagtccacg cccgcggtgt ggaagggctg gatctgggtg
     3001 gggacccgcg ccggtccgat ctacgggatc gccgaccccg gcgcctgagt ccgtgccgcc
     3061 gacgggcctc tccttccgcc cgcggacgcg ggtgcgctac ggccgcatcc tggtcgtgct
     3121 ggcggctctc ggcggactgg tggccttcgt ctggctcggg cccttccgcg gcgacggatc
     3181 gcccgccgtc gatgcctcac cgtcggccgg gggtgcgagc gatcgtccgc cgatgtgcgc
     3241 gcaggcggac gcgccggccc ggctcgccgc ggtgggcgac tgggagcaca ccctcgtcga
     3301 cactcggttc acgctcccgg cggcctacgt tccgcccggc ctggtctcca cgaccgaggc
     3361 cgggttcgac ggcgcgttcc tggtgcgcga cttcgtcgtg cccgacctga tcgcgctgcg
     3421 ccgggccgcc gaggccgccg gcaacccggt cgaggtgatc gcttcctacc gctcgtacga
     3481 cgaccaggcc gagctgttcc agcggcaggt ggaccgattc ggcgaggagg tggcgctgca
     3541 gcgcacggca cggcccggcc actcggagca ccagctcggg acgacggtcg acttcaagac
     3601 caggggggag gacgacgtca gtccgtactg ggaggacacc cccgccgggg cgtggatggc
     3661 cgggcacgcc gtccagtacg gcttcgtgat gtcatacccg cgcgggaagg acgcggtgac
     3721 ctgctacgcg tacgagccgt ggcacttccg atacttcggc cgcgggcgcg ccgcgaagat
     3781 cgccgccagc ggcctcaccg tccgcgagtt cctgtggcgc gagcagcgcc ccgccacccg
     3841 ggcgacgccg tcgccctccg cctcgccctg agcgagccgg cgccgggcca gcggtgagcg
     3901 ttccgcgatc tcccccgccg ggttcggctt gacaggcaag caacaacttg cctatcatcc
     3961 gggtggtggg ggacgtgtat cgggcgctgg ccgacccgac acggcggacg atcctcgacg
     4021 agctgaccga gcgtgacggc caaacgctct tcgagatctg cacccggttg acgatgaagc
     4081 acgggctgag ctcgtcccgc caggcgatct cacagcatct cgaggtgctc gaggcggccg
     4141 gtctggtccg gacgaagcgg gagggccgct acaagttcca tttcatcgag acgacgccgc
     4201 tgagggccgt ggtcgagcga tggccgatcg accgagggga ggaccaccga tgaggatcaa
     4261 cgtcacgggc gtgtacgtgg acgatcagca caaggcgctg cggttctaca ccgaggtgct
     4321 cgggttcgtg aagaagaccg agatcccgct gggggaggcg agctggctta ccgtcgtgtc
     4381 gccggacgag ccccacggca cggagctgat cctcgagccg gacgcccacc ccgccgtccg
     4441 gccgttcaag gaagcgctcg tggacgacgg gatcccgttc acctcgttcg ccgtcgacga
     4501 cgtgcggacg gagtgcgagc gactgcgatc gctcggtgtc cggttcacgc aggagccgct
     4561 cgcgatgggg cccgtcacca ccgcggtctt cgatgacacg tgcgggaacc tcatccagat
     4621 cgcgacggcg cgtgaggcga ccggctagcc gcggaggtgg cggcggagcg cggccaggcg
     4681 ctcgtcccac tcggcgccga ccccggccat ccagcccatc gcctccgcca tcggtcgcgg
     4741 cgtgaggcgg tagcgcaccg cccggccgat ccgctccgac tcgaccaggc cggcctcggc
     4801 cagtgccgcg aggtgcttcg acacggcctg tcgcgtcacc aggaactcgc cggccagctc
     4861 cgtcgccgtg gccccctcgc cgtgctccga cagcgcccgc accacggctc gccgggtggg
     4921 atccgacagc gcctggaaca cggcggccac gcgctcgtcg cgtccgctca cggccgggct
     4981 cccgctggcg atgcctcgac gacggtctcg gtgaccgtga gccgggttcc gccgtggtcc
     5041 tcctccagct cgaacgccac gcgcgtcggc tcggtcggct ccgcggaccc gtccgggcgg
     5101 cgcctgaacg gagcccagcg gaaggcgaac cggcgtggtc gatccgactc ttcgaccatc
     5161 atcgcccgct cggagccgtc ggggaagcgg aagcggccgt ggccgcccgg cctgggatcg
     5221 agctccacgt tggccccgaa ccacgcgccg aggcggccgg gatcggtcag cgcccgccat
     5281 acctcgccgg gcgggacggg gaggacgatc tcccgttcca cgcgtccccg gctcaacgcg
     5341 caactccccg gttgcggatc atgcgccacc acgctagagt gcaacccatc ggttgcgagt
     5401 ctacaccgag cggaggtgac gccgtgagcc agggcctggt gccgtacgtc gtggaggaga
     5461 ccgcccgcgg cgagcgctcg ttcgacatct actcccgtct gctcaaggag cgggtcgtgt
     5521 tcctcggccg cgaggtcgac gacgacctgg cgaacctgct gaccgcgcag ctgctgcacc
     5581 tcgaggccga ggacccggag cgcgacatca atctgtacat caactcgccg ggtggctcgg
     5641 ccacggcgct gttcgcgatc tacgacacga tgcaattcgt caagcccgac gtcgcgacga
     5701 cctgcgtcgg ccaggccgcc agcgccgccg ccgttctgct cgcagccggc gcgctcggga
     5761 agcgcgcctg cctgccgaac gcgcgcgtcc tgattcacca gccctggggc caggtcggcg
     5821 gccaggcggt ggacatggag atccacgccc gcgaggcgat ccgccaccgc cggctgatgg
     5881 acgagatcct ggcgcgccac accggccagc ccgtcgagaa ggtgtcgcac gacaccgacc
     5941 gcgacttcat cctcaccgcg gagcaggcca aggactacgg cctggtcgac atgatcctgg
     6001 cccgccgcgt cggccccctc gccgccttcg agcccgacga gacggccccc gagaacggcg
     6061 accgcgcggg gtgactcgcc ggatctgccg taggctgccg ggatggcgcc gatccagccc
     6121 ggaacgaaag ccccgccggc tcccggagtc gcgttcgacg ggcccacggc gctgatcttc
     6181 tacaaggtga cctgccctgt ctgccagctg gccgggccgc cgctcgaccg gttgcgcaac
     6241 gcgttcccgg acgcggtgat cgcggtcggc caggacccgc cggagaagct cgaggcattc
     6301 gcacgcgagt ggcgtttccg agtcccggcg gtcagcgacc cgccgccgta tccgatctcg
     6361 gacgcgtacg ggatccgcac ggtgccgacc gtgttcctgg tggaccgcga cgggaccgtg
     6421 ctcgagacgg tcgagtcgtg ggaccgcgag ggcttcaacc gcgtgtcgcg acgcctggct
     6481 gagctgaccg ggaccgagta tgcggaggtg tcgcacccgt ccgacgggct gcccgccttc
     6541 cgtccggggt gagggtcgcg caacgcccac taggtcagtg ggtgtcagga ttcggggccg
     6601 atccgctcct gtgtggtagg agggccggac gtgctcgtgg actcgagacc ccaggacgag
     6661 ctggagcgtc tgtaccggga ccagcgggac cggatgtggc aggccgtgtt cgcgttcgcc
     6721 ggcgatcgag acgtggcgag cgatgcggtg gccgaggcat tcgcccaggc gctccgacga
     6781 ggcgacgccg tccgctcgcc ggagcggtgg atctggcgga cgatcttccg gatcgccgca
     6841 ggagacctga aggagcggcg ccgtcaccag tcacacccgg tggagcgcac ctacgagatg
     6901 gaagagctgg cgcgggacct ggtggcggcg ctggggaagc tcccccccag gcaacgggcc
     6961 gccgtggtgc tccatcacgc ggtcgggtat ccggcggccg aggtggcccg gatcctcggc
     7021 tccacgacgg ccgcgatcca cgtgcagatc agccgggggc ggaagcgcct gcgcgagctc
     7081 ctggagacag atgatgccta gcctggacga tcacttccgc gccctgaccg aggtccgccc
     7141 accgagtaac tggcccgatc tggacccgcg tggaccgcgc cccccgtatg cacggtcgct
     7201 cttccgacgt cggatcggcg tggctgccct ggccgttctc atcgcggccg cgggattcgt
     7261 cgtcgcggtg ctggcgttcc agcggccggc ccccggaccg gcgacgagct catcggtccc
     7321 ggtacctcct caccctccac cgagcccgac gaccggttca ccgacgccga cggcgatgga
     7381 cggcgtgttc gccgccatgc tcgatgccat tcgaagctcc agcccccccg gctggctgtt
     7441 cgccctgcag cccgaccggc tggacggtga ctggcggatg gacggcgcgg tggacgatgg
     7501 ctccgggccg gggcggctgt tcgtggtcgt gaccatccgc ccgggcatga ttcaggcaca
     7561 cccgtgcacg gacatggagt ttcggcagag aggtcgatgc gtcgagcgga cgcttcccgg
     7621 tggcgaccgg ctcgtgctcc gggacgtcgt cgtcgaggcc ggcgggatga agaccatcgt
     7681 cgccgtgctc gtccaccccg acggctcggg tgtctcggcg gaggc
//
LOCUS       DASPWO010000392         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_314294, whole genome shotgun sequence.
ACCESSION   DASPWO010000392 DASPWO010000000
VERSION     DASPWO010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314294"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..>2271
                     /locus_tag="VEO00_09020"
     CDS             50..>2271
                     /locus_tag="VEO00_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain-containing protein"
                     /protein_id="HYU58176.1"
                     /translation="MRASFADSTGGAGCRRGARGGAPRGAAALLRATAVAIAIALLAL
                     PGPSQAQVAISGAFTNVGNWSGTSTNPPAIPASPNILSPTAANRVLFVAVLVKANGPF
                     SDAGIFTVTGKYNTTVDVLQSAQSGAATGTSTTAVWLGFVKEGNLVAGGSSAPLSITI
                     VSKSGTAITGASIWTAFFTGVSSGVPAWTVSESSSVSSTASPATLPGTIYSPPAGALV
                     AVAAAPTAQPAIALGYTLSAGFAPAGAVPLGGVIAYKTTASAVTETTAATGTAPLALA
                     VMSLNPASYVGSGDGTPSSILPVVAILNPANGQKLSRSWAAGKLGFKVQARVFSPKNG
                     VATQTISNVSLLENDLAVATLTQSPKYVGTTAESGIWEVIYNPAAPDGTSLKLQVQAT
                     NVPSGTVKSAPVTVTTAAAHSGDGNLLARDNSSQLCNDCHALPLHSAETAGNRLGSWA
                     VVCRDCHTPHGTTNIFLVPTSVTPPPVTRSDAAMVTPKTVQFYSPARDVTAGIVNANR
                     NGPCQACHTRTSDGVGAPLFRNNIPLVTAHNAGSECMTCHSHQSGFPKPAAGASCHDC
                     HGSAEVPRTDLMPAETVPPIDTCNNTVGKVAGNLPGPNRVGAHKAHLLGQTFTKTGVI
                     TCDQCHLTNGSGGATPHAGHPGANCSTATADRAVFTFGTIATGTATPTWTTAVTPTYS
                     YSTTQTCGTVYCHGAFRNSGIVATPGWNNTVTCGGCHRNSNGTSAAPSFPHPARASDL
                     T"
BASE COUNT          349 a          870 c          763 g          289 t
ORIGIN      
        1 caccgtcatg gtctcggtgc ggagccaggg gctcttcggg ggcagcatgg tgagggcgtc
       61 gttcgcggat tcgacgggcg gggccgggtg ccgtcgaggg gcgcgtggcg gagcgccgcg
      121 cggcgccgcg gcgctgctcc gggcgaccgc ggtcgcgatc gcgatcgcgc tcctcgcgct
      181 ccccgggccg tcgcaggcgc aggtcgcgat ctcgggggcg ttcaccaacg tcgggaactg
      241 gagcgggacc tcgacgaacc ctcccgccat cccggcgagc ccgaacatcc tgtcgccgac
      301 cgccgcgaat cgcgtcctct tcgtcgcggt cctcgtcaag gcgaatggcc cgttctcgga
      361 cgccggcatc ttcaccgtca cggggaagta caacacgacg gtggacgtcc tccagagcgc
      421 gcagtcgggc gccgcgaccg ggacctcgac gaccgcggtg tggctcggct tcgtgaagga
      481 ggggaatctc gtcgccggcg ggtcgagcgc cccgctcagc atcaccatcg tctcgaagag
      541 cggcaccgcc atcaccggcg cgtcgatctg gaccgcgttc ttcacgggcg tgagctccgg
      601 cgtcccggcg tggacggtct cggagagcag cagcgtcagc tccacggcga gcccggcgac
      661 cctccccgga acgatctact cgccgcctgc cggcgccctc gtcgcggtcg ccgcggcgcc
      721 gaccgcgcag cccgccatcg ccttggggta cacgctgtcc gccgggttcg cgccggccgg
      781 ggccgtcccg ctcggcgggg tcatcgcgta caagaccacc gcctcggcgg tcacggagac
      841 gaccgccgca acggggaccg cgccgctcgc gctggcggtc atgtcgctca acccggcgag
      901 ctacgtgggg agcggggacg ggacgccgag cagtatcctg cccgtggtcg cgatcctgaa
      961 tccggcgaac ggccagaagc tgtctcggtc ctgggccgcc ggcaagctcg gcttcaaggt
     1021 gcaggcgcgc gtgttcagcc cgaagaacgg cgtcgccacg cagacgatct cgaatgtctc
     1081 gctgctcgag aacgacctcg ccgtggccac cctcacgcag agcccgaagt atgtgggcac
     1141 caccgccgag tccgggatct gggaggtcat ctacaacccc gccgccccgg acggcacgtc
     1201 gctgaagctc caggtgcagg ccacgaacgt cccgtccggc acggtgaaat cggcgccggt
     1261 gaccgtcacg acggccgccg cccacagcgg tgacgggaac ctgctcgccc gcgacaactc
     1321 aagccagctc tgcaacgact gccacgcgct gccgctccac tccgcggaga cggccggcaa
     1381 ccggctcggc tcgtgggccg tcgtctgccg cgactgccac acgccgcacg gcaccacgaa
     1441 catcttcctc gtgcccacgt cggtcacgcc gcccccggtc acgaggtcgg acgccgcgat
     1501 ggtgaccccg aagacggtcc agttctactc cccggcgcgc gacgtcaccg ccggcatcgt
     1561 gaacgcgaac cggaacggcc cctgccaggc gtgccacacc cgcacctccg acggcgtcgg
     1621 cgctccgctc ttccggaaca acatccccct cgtgacggct cacaacgcgg ggtcggagtg
     1681 catgacctgc cactcgcatc aatccgggtt cccgaagccg gcagccggcg cgagctgcca
     1741 cgactgccac ggctccgcgg aggtgccccg gaccgacttg atgccggccg agacggtccc
     1801 gccgatcgac acgtgcaaca acaccgtggg gaaggtggcg gggaacctcc cgggaccgaa
     1861 ccgcgtcggc gcgcacaagg cgcacctcct cgggcagacc ttcacgaaga cgggcgtcat
     1921 cacctgcgac cagtgccact tgaccaacgg ctcggggggc gcgacgccgc acgccggaca
     1981 cccgggagcg aactgctcga ccgccaccgc ggatcgcgcg gtgttcacct tcggcaccat
     2041 cgcgaccggg acggcgacgc cgacgtggac caccgcggtg acgccgacct acagctactc
     2101 gaccacccag acctgcggca cggtctactg ccacggcgcc ttccggaact cggggatcgt
     2161 cgcgacgccg ggctggaaca acacggtcac ctgcggcggc tgccaccgca actcgaacgg
     2221 cacgagcgcc gcgccctcgt tcccgcaccc cgcgcgcgcg agcgacctga c
//
LOCUS       DASPWO010000393         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_227325, whole genome shotgun sequence.
ACCESSION   DASPWO010000393 DASPWO010000000
VERSION     DASPWO010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227325"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VEO00_09025"
     CDS             complement(<1..399)
                     /locus_tag="VEO00_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HYU58177.1"
                     /translation="MTEPVMAGGHRRIDRVMDPMFLDGLGDARIEELRRRRDECRAEI
                     EYLSYLRRLLQGRLDILRAERGQRESGAAGSLVDRLPEILSGGGATGSRGAFVHVVLP
                     DEEMALARRHVERLVSDAGLSDLPALSDAAL"
     gene            complement(396..1238)
                     /locus_tag="VEO00_09030"
     CDS             complement(396..1238)
                     /locus_tag="VEO00_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HYU58178.1"
                     /translation="MVPVDPVIITVAPVGGELSRAEQPNLPLTAEEIGAEVARSAEAG
                     ASIVHVHARDEAGEPTQSREAFARIIEAIRARCDIVVQTSTGGSVGMSEAHRSEPLDL
                     RPEMATLTTGTVNFGDEVFQNPFPMVERIFLAMRERGILPEFEIFDSGMVDTALRLVA
                     AHDATPHRLHFDFVLGVPGGMRGSPGAVAFLSSLLPDGATWTATGIGRTHLPVTLAAL
                     ALGGNVRTGFEDTIYYARGELAASNADLIARVARLTREAGRAVATPAQAREMLGLGDL
                     GGRG"
     gene            1346..>2719
                     /locus_tag="VEO00_09035"
     CDS             1346..>2719
                     /locus_tag="VEO00_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HYU58179.1"
                     /translation="MLALSSLSWGVVVVVAGAVVLYLLMSVKIIGPTEVGLVGKRIGR
                     KLAEGHVIAFAREAGYQADLLMPGIRFKPWLLYSVRKHPWVQVPADGIGVVIAQVGRP
                     LPAGAKSAEYRQAFGNFRDVRSFVQNGGQQGVQRPVLPPGSLIPLHPVGFLVMTYSRT
                     YGLPVASDFLPQPTRKGVLTYESFGLAQEELKVVRIEPAGNVDYIGVVTTQEGDPLPS
                     GDIAGRLGGFDDVAAMEAAGRTDGEIIEVLLGNKNGLHNNYQDFQAFLDNGGKIGLQH
                     DPLLYGAFLLNPFLVKVERVPMLVVNQGEVAVIKSYLGLSTQDTSGEEFKFGSIVRPG
                     HRGIWREPLRTGKFPINPRVYQAEIVPTAILTLNWAEATSHAHNLDAQLSSIDAKSQE
                     GFTFTIDLQVQIHVPDTKAPSVISMVGTMLNLVNEVLQSAVGNYFRNTLQGLPAVGFI
                     QRRQEVQD"
BASE COUNT          412 a          952 c          978 g          377 t
ORIGIN      
        1 gagggccgcg tccgacaggg cgggcagatc cgacaggccg gcgtcggaga cgagccgctc
       61 gacgtggcgg cgtgccagcg ccatctcctc gtccggcagg acgacgtgca cgaacgcgcc
      121 gcgcgatccg gtggcgcccc cgccggagag gatctcgggg agccggtcga ccagcgagcc
      181 ggcggcgccc gactctcgct gcccgcgctc ggcacgcagg atgtcgagcc gtccctgcag
      241 caggcgccgc aggtacgaga ggtactcgat ctcggcccgg cactcgtcgc gccggcggcg
      301 caactcctcg atgcgggcgt ctcccaggcc gtcgaggaac atcgggtcca tgacccggtc
      361 gatccggcgg tgcccgcccg ccatcaccgg ctccgtcatc cccgtccccc caaatccccc
      421 agcccgagca tctcgcgcgc ctgggcgggc gtggcgaccg cgcggccggc ctcgcgggtc
      481 aggcgggcca cccgggcgat caggtcggcg ttcgaggcgg ccagctcgcc ccgtgcatag
      541 tagatggtgt cctcgaagcc cgttcggacg ttgccgccga gggccagcgc cgccagcgtc
      601 accggcaggt gtgtgcgtcc gatgccggtg gcggtccagg tggccccgtc gggcaggagc
      661 gaggagagga acgcgacggc cccgggcgac ccgcgcatcc cgcccggaac gccgagcacg
      721 aagtcgaagt ggagacggtg cggcgtcgcg tcgtgcgccg ccaccaggcg cagcgcggtg
      781 tccaccatcc cggagtcgaa gatctcgaac tccggcagga tccctcgctc gcgcatggcc
      841 aggaagatcc gctcgaccat cgggaacgga ttctggaaga cctcgtcccc gaagttcacc
      901 gtgccggtgg tgagcgtcgc catctccggt cgcaggtcga gcggctcgga gcggtgcgcc
      961 tcgctcatcc ccacagagcc gccggtggag gtctgtacga cgatgtcgca gcgggcccgg
     1021 atggcctcga tgatccgggc gaacgcctcg cgggactggg tcggctcccc cgcctcgtcc
     1081 cgggcgtgga cgtggacgat cgaggcgccg gcctccgccg agcgcgccac ctccgccccg
     1141 atctcctccg ccgtcagcgg caggttcggc tgctccgccc gcgagagctc cccgccgacc
     1201 ggggccaccg tgatgatcac cgggtccacg gggaccatga tggaacggcg acgggatcgg
     1261 gtccaggacg ccaggacgcg accgcgaccg gccccgcacc gcggtcccga gcggtaccgt
     1321 gatggccacc gaggaaggag gggccatgct cgcgctgtcg agcttgtcgt ggggtgtcgt
     1381 cgtggtggtg gcgggggccg tcgtcctcta cctgctgatg tcggtgaaga tcatcgggcc
     1441 gaccgaggtg gggctggtgg gcaagcggat cggcaggaag ctcgccgagg gtcacgtcat
     1501 cgcgttcgcg cgcgaggccg ggtaccaggc cgacctgctg atgccgggca tccgcttcaa
     1561 gccgtggctg ctgtacagcg tgcgcaagca cccctgggtg caggtgccgg ccgacgggat
     1621 cggcgtggtc atcgcccagg tcgggcggcc gctcccggcc ggggccaaga gcgccgagta
     1681 ccggcaggcg ttcggcaact tccgggacgt gcggtcattc gtgcagaacg gcggtcagca
     1741 gggcgtccag cgcccggtcc tgccgccggg ctcgctgatc ccgctgcatc cggtgggctt
     1801 cctggtgatg acgtacagcc gtacctacgg cttgccggtg gcgagcgact tcctgccgca
     1861 accgacccgc aagggcgtcc tgacctacga gtcgttcggc ctggcgcagg aggagctgaa
     1921 ggtcgtccgc atcgagcccg ccgggaacgt cgactacatc ggcgtcgtca cgacgcagga
     1981 gggcgacccg ctcccgtccg gcgacatcgc ggggcggctc ggggggttcg acgacgtggc
     2041 cgcgatggag gcggcggggc gcaccgacgg cgagatcatc gaggtgctgc tcggcaacaa
     2101 gaacgggctg cacaacaact accaggactt ccaggccttc ctggacaacg gcgggaagat
     2161 cggcctgcag cacgacccac tgctctacgg agcgttcctc ctcaacccct tcctggtcaa
     2221 ggtggagcgc gtgccgatgc tcgtcgtcaa ccagggcgag gtggcggtga tcaagtccta
     2281 cctcggtctc tcgacgcagg acacctccgg cgaggagttc aagttcgggt cgatcgtgcg
     2341 gcccggacac cgcggcatct ggcgcgagcc gctccgcacc ggcaagttcc cgatcaaccc
     2401 gcgcgtgtac caggccgaga tcgtcccgac cgcgatcctg acgctcaact gggcggaggc
     2461 gacctcgcac gcgcacaacc tggacgcgca gctcagctcg atcgacgcga agagccagga
     2521 ggggttcacg ttcacgatcg acctgcaggt gcagatccac gtccccgaca cgaaggcgcc
     2581 gagcgtgatc agcatggtcg gcacgatgct caacctggtc aacgaggtgt tgcagtcggc
     2641 ggtgggcaac tacttccgga acaccctgca ggggctgccg gcggtcgggt tcatccagcg
     2701 ccgccaggag gtgcaggac
//
LOCUS       DASPWO010000394         2766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_314990, whole genome shotgun sequence.
ACCESSION   DASPWO010000394 DASPWO010000000
VERSION     DASPWO010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314990"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VEO00_09040"
     CDS             <1..401
                     /locus_tag="VEO00_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58180.1"
                     /translation="SWHHGHHGRPGHHHHDRPEPVDTVPGYRRRTAFGIGMIHGLGAE
                     TPTQVLVFLAAAGAGGGAVGVAVLVAFIAGLLASNSLITLGSAMGFLSASKNFAVYAT
                     VAVVTAVFSLVLGTLFVLGKESVLPSFFGG"
     gene            407..1168
                     /locus_tag="VEO00_09045"
     CDS             407..1168
                     /locus_tag="VEO00_09045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYU58181.1"
                     /translation="MTEAFARNPRHDVHERIRAFWDADAETYDHAPGHAVTDPVIAAA
                     WRDTLHRHLPAPPVRVLDVGAGTGALSLMAAELGHRVTALDLSPGMLARLQEKAQARG
                     LEIETVVGPADEPPPGPFDAILERHVAWTLPDAVGAFHAWREALAPGGRLVLYQGLWG
                     GPRRLRRLAAAAVRRLKRVPHDHHAEYDDELRGAMTFGHGVTPDGLAAALARAAWRRY
                     RVERLWDVERAERAAARPVLRHLEYVPRFALLAEA"
     gene            1266..1781
                     /locus_tag="VEO00_09050"
     CDS             1266..1781
                     /locus_tag="VEO00_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58182.1"
                     /translation="MVQAFYGPVSTLCFTLLGLWWVAVQIRGGELFANAEWRRTVYGI
                     SVYFMLPGLMSLLALVSPGVSALWRVAFVLAGLLGATESALALRSTRFMAGRSSRMTA
                     ARWATIALYVLIALVGAAPEVASDLADLPPLLVEGILVSLMIVLGANLAWMALVHAAE
                     SRTPLPPSSAG"
     gene            complement(1778..>2766)
                     /locus_tag="VEO00_09055"
     CDS             complement(1778..>2766)
                     /locus_tag="VEO00_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYU58183.1"
                     /translation="LSVSGYHIREAGANAWQELAYTLADGFAYVELGQQRGLDVDRFA
                     PGLSFFFNAHIDFFEEIAKYRAARRIWARWMKDRYGAADERSLRLRFHTQTAGCSLTA
                     QQPENNIVRTAIEALAGVLGGTQSLHTNALDEVLALPTEKAAEIALRTQQIIAHETGV
                     ANVIDPLGGSYFVEALTNRMQERAEEYFARIEEMGKGSMLEGVLAGIERGFFQSEIAD
                     AAFREQMRYEKGRLIKVGVNEFVNPNERPYDMLVIPPDTERTQVASLDRTRRERDGSV
                     VTASLERLRATAATDENLIPLLVDCARAYCTEGEIVETLKVPFGEYTETPRF"
BASE COUNT          333 a         1041 c          957 g          435 t
ORIGIN      
        1 cctcctggca ccacggccac cacgggcggc ccggccacca ccatcacgac cgtcccgagc
       61 cggtcgacac cgtacccggc tacagacggc ggaccgcctt cgggatcggc atgatccacg
      121 gcctcggcgc cgagaccccc acccaggtgc tcgtcttcct ggccgccgcg ggcgccgggg
      181 gcggggcggt cggcgtcgcc gtcctggtcg cgttcatcgc cggcctgctg gcgtcgaact
      241 cgctgatcac gctgggctcg gcgatgggct tcctgtccgc ctcgaagaac ttcgcggtgt
      301 acgccacggt ggcggtggtc accgcggtct tcagcctggt cctgggcacg ctcttcgtcc
      361 tgggcaagga gagcgtcctc ccgtcgttct tcgggggctg acgcgggtga cggaagcttt
      421 cgctcggaat cctcgccacg acgttcacga gcggatccgc gcgttctggg acgcggacgc
      481 cgagacctac gaccacgcgc cgggccacgc ggtgaccgac cccgtgatcg cggcggcctg
      541 gcgcgacacc ttgcaccgtc acctccccgc tcccccggtc cgggtgctcg acgtcggcgc
      601 gggaacgggc gcgctgtcgc tgatggccgc cgagctcggg caccgggtca cggccctcga
      661 cctgtccccc gggatgctcg cccggctgca ggagaaggcc caggcgcgcg ggctggagat
      721 cgagacggtg gtcggccccg ccgacgagcc gccgcccggg cccttcgacg cgatcctcga
      781 gcgccacgtc gcctggacgc ttccggacgc cgtcggcgcg ttccacgcct ggcgcgaagc
      841 gctcgccccc ggcggccgcc tcgtcctcta ccagggactg tggggcggac cccgccggct
      901 gcgccgcctc gccgccgccg ccgttcgtcg cctgaagcgc gtcccgcacg accaccacgc
      961 cgagtacgac gacgagctgc gcggcgccat gaccttcggc cacggcgtca cgccggacgg
     1021 gctggccgcc gccctggccc gggcggcgtg gcgccggtac cgcgtcgagc gattgtggga
     1081 cgtggagcgg gccgaacgcg cggcggcccg ccccgtcctg cgccacctgg agtacgtccc
     1141 gcgcttcgcc ctcctcgcgg aggcctgacg gagccacgga cgacgccggg acgttccggc
     1201 acgcctcctt gacgcccgtg gccccggcgg cctacacacg tgccacacga cgcgggaggt
     1261 gaccggtggt tcaggccttc tacggcccgg tgtcgacgct gtgcttcacg ctcctcggcc
     1321 tgtggtgggt ggcggtgcag atccgtggcg gtgaactgtt cgcgaacgcc gagtggcggc
     1381 gaaccgtcta cgggatctcc gtctacttca tgctgccggg gctgatgagc ctgctggccc
     1441 tggtctcgcc gggcgtctcc gccctgtggc gggtcgcgtt cgtcctcgcc ggcctgctcg
     1501 gggcgacgga gtcggcgctc gccctgcgct ccacgcgatt catggccggg cgctcgagcc
     1561 ggatgacggc cgcccgctgg gccaccatcg cactctacgt gttgatcgcg ctggtcggcg
     1621 ccgcccccga ggtcgcgtcg gacctggcgg acctcccgcc gctcctggtg gagggcatcc
     1681 tggtctcgct catgatcgtc ctgggcgcga acctcgcctg gatggccttg gtccacgccg
     1741 ccgagtcgcg gacaccattg cccccgtcga gcgccggcta gaagcgcggg gtctcggtgt
     1801 actcgccgaa ggggaccttc agcgtctcga cgatctcgcc ctcggtgcag taggcgcggg
     1861 cgcagtcgac cagcagcggg atcaggttct cgtcggttgc ggcggtcgcg cgcagccgct
     1921 ccagggacgc ggtgacgacg gacccgtcac gttcacgccg cgtccgatcg agcgaggcga
     1981 cctgcgtacg ctcggtgtcc ggcgggatca ccagcatgtc gtacggccgc tcgttcgggt
     2041 tcacgaactc gttgaccccc accttgatca gccggccctt ctcgtagcgc atctgctcgc
     2101 ggaaggcggc gtcggcgatc tcggactgga agaacccccg ctcgatcccg gccagcactc
     2161 cctcgagcat cgagcccttc cccatctcct cgatccgggc gaagtactcc tcggcgcgct
     2221 cctgcatccg gttcgtcagc gcctcgacga agtaggagcc gccgaggggg tcgatcacgt
     2281 tcgccacacc ggtctcgtgg gcgatgatct gctgggtccg cagcgcgatc tcggccgcct
     2341 tctcggtggg cagcgccagc acctcgtcga gcgcgttcgt gtgcagcgac tgcgtcccgc
     2401 cgagcacgcc ggccagcgcc tcgatcgccg tgcgcacgat gttgttctcc ggctgctgtg
     2461 ccgtcagcga gcacccggcg gtctgggtgt ggaagcgcag ccgcagcgac cgctcgtccg
     2521 ccgcgccgta gcggtccttc atccagcgcg cccagatccg tcgcgcggcg cggtacttcg
     2581 cgatctcctc gaagaagtcg atgtgcgcgt tgaagaagaa ggacagcccg ggcgcgaacc
     2641 ggtccacgtc cagaccgcgc tgctggccga gctcgacgta tgcgaacccg tccgcgagcg
     2701 tgtacgcgag ctcctgccac gcgttcgccc cggcctcccg gatgtggtag ccggagacgc
     2761 tgaggg
//
LOCUS       DASPWO010000395         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_58180, whole genome shotgun sequence.
ACCESSION   DASPWO010000395 DASPWO010000000
VERSION     DASPWO010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58180"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VEO00_09060"
     CDS             <1..577
                     /locus_tag="VEO00_09060"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU58184.1"
                     /translation="RHQELQWTEHLVEHIEDDLERSGRLGRPERLPAVAFVDLTGYSR
                     LTEERGDQAAAELASQLAAVVNRETRAHGGTPVKWLGDGVMVALRDSAGAVRSALGLV
                     RAVPAAGLPPAHIGLAAGRVVAYGGDYFGRTVNLAARLSAHAGPGQVLVNEAVTEQAA
                     TAEATFTEIGPVHLKGFSEPIRVFETTPTST"
     gene            complement(594..1151)
                     /locus_tag="VEO00_09065"
     CDS             complement(594..1151)
                     /locus_tag="VEO00_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HYU58185.1"
                     /translation="MAKLIYSTISSLDGYVADRDGSFDWAAPDEQVHTFVNDLERRVG
                     TYLYGRRMYEVMVAWETMDTADQPPFVQDYAEIWRAADKIVFSTTLDAVTSARTRIER
                     AFDPEAVRQVKASAGRDISVGGPELAAHAFEAGLVDECHLFLTPIVVGGGTRSLPDDV
                     RFWLDLVDERRFGSGVVHLHYGVGS"
     gene            1301..2080
                     /locus_tag="VEO00_09070"
     CDS             1301..2080
                     /locus_tag="VEO00_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU58186.1"
                     /translation="MTTVEKHEPGTFCWVELATRDLDAGREFYSSIFGWQSDARPVEG
                     WGEYATFTVADGRSAAGGYAQQDGERSMGVPPHWNLYVSTDDVDSDVAKATELGGQAT
                     IPGMDTPLGRMAVIADPTGARFCLWQSEQMPGFAVRGEHGAFTWADLITPDKDKAQEF
                     YGGLFGWTGEEQGEEFGYYTVVKKGDEQIGGFMNPMQPGMPANWMPYFAVTGAEAVTN
                     AATGAGAQVKMPPTPIPTVGTFAVMADPQGAVFAILQAEPQ"
     gene            complement(2381..2488)
                     /locus_tag="VEO00_09075"
                     /pseudo
     CDS             complement(2381..2488)
                     /locus_tag="VEO00_09075"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
     gene            complement(2698..2934)
                     /locus_tag="VEO00_09080"
     CDS             complement(2698..2934)
                     /locus_tag="VEO00_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HYU58187.1"
                     /translation="MSRVLRSTLVLSEVIALGLVVTPLRSAEALAPGPFEVTKTADTY
                     DGACDGDCSLREAVVAANDRSGADVIVVPAGPTR"
     gene            3104..>3597
                     /locus_tag="VEO00_09085"
     CDS             3104..>3597
                     /locus_tag="VEO00_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU58188.1"
                     /translation="MSYAIVAEGLVKRFGETTALAGADLAARAGTVLGVLGPNGAGKT
                     TAVRILATLTTPDSGRASVGGYDVVAQAHQVRQLIALTGQYAAVDEGLTGFENLLMIG
                     RLLGRSRPEAKGRAGDLLERFELAEAGDRPVRTYSGGMRRRLDLAASLVGRPAVLYLD
                     EPTTG"
BASE COUNT          531 a         1255 c         1294 g          517 t
ORIGIN      
        1 gcggcaccag gagctgcagt ggaccgagca cctggtcgag cacatcgagg acgacctgga
       61 gcgctccggg cgcctgggcc ggcccgaacg cctccccgcc gtggcgttcg tcgacctcac
      121 cggctactcg cggctgaccg aggagcgagg cgaccaggcc gcggcggagc tggcgtccca
      181 gctcgcggcc gtggtcaacc gtgagacacg cgcccacggc ggcacgcccg tcaagtggct
      241 cggcgacggc gtgatggtgg cgctccgcga ctctgccggc gctgtgcgct ctgcgctcgg
      301 cctggtccgc gccgttcccg cagccggcct cccgcccgcg cacatcgggc tggccgccgg
      361 ccgcgtggtt gcctacggcg gcgactactt cggccgaaca gtgaacctgg cggcccggct
      421 ctcggcccac gccggccccg gccaggtcct ggtgaacgag gcagtgaccg aacaggccgc
      481 caccgccgaa gccacattca cggagatcgg cccggtgcac ctcaagggct tctccgagcc
      541 catccgcgtc ttcgagacca cgccgacgtc aacctgaccc cacctccgag ggcttacgaa
      601 ccgacgccgt agtggaggtg gacgacgccg ctgccgaagc ggcgttcgtc caccaggtcg
      661 agccaaaacc ggacgtcgtc ggggagcgac cgtgtgccgc cccccaccac gatgggcgtg
      721 aggaacagat ggcactcgtc gaccaacccg gcctcgaacg catgggcggc gagctcgggg
      781 ccgcccacgc tgatgtcgcg tcccgccgac gccttcacct gccggaccgc ttcagggtcg
      841 aaggctcgct cgatccgcgt cctggcgctg gtgaccgcgt ccagggtcgt ggagaacacg
      901 atcttgtccg ccgctcgcca gatctccgcg tagtcctgca cgaagggcgg ctggtcggcg
      961 gtgtccatcg tctcccaggc gaccatcacc tcgtacatcc ggcgcccgta caggtaggtt
     1021 ccgacccgcc gctcgaggtc gttgacgaac gtgtgcacct gctcgtccgg cgccgcccag
     1081 tcgaagctgc cgtctctgtc cgcgacgtag ccgtcgagcg acgagatcgt cgagtagatc
     1141 agcttggcca tggcgtgcct cccggactca gcgccgatcg cctttcgctc ccaccccagg
     1201 atggcatccc caccgtccgt cgagcatcgg cgcggggtgt aattcgtgtg ggcgttctgg
     1261 catagggttg agtcgaacga cgagcaaggg aggaattcgc atgacgacgg tcgagaagca
     1321 cgagccgggc acgttctgct gggtggagct cgccacccgc gacctggacg ccgggcggga
     1381 gttctactcc tcgatcttcg ggtggcagag cgatgcccgt ccggtcgagg ggtggggcga
     1441 gtacgcgacg ttcacggtcg ccgacggccg gagcgccgcc ggcggatacg cgcagcagga
     1501 cggggagcgt tcgatgggcg tcccgccgca ctggaacctc tacgtctcca ccgacgacgt
     1561 cgactccgac gtcgcgaagg ccaccgagct cggcgggcag gcgacgatcc cggggatgga
     1621 cacgccgctc ggacgcatgg ccgtgatcgc cgatcccacc ggagccaggt tctgcctgtg
     1681 gcagtcggag cagatgcccg ggttcgcggt ccgaggcgag cacggcgcct tcacgtgggc
     1741 cgacctcatc acgccggaca aggacaaggc gcaggagttc tacggcgggc tgttcggctg
     1801 gaccggggag gagcagggcg aggaattcgg gtactacacc gtcgtcaaga agggcgacga
     1861 gcagatcggc ggcttcatga acccgatgca gccgggcatg ccggccaact ggatgccgta
     1921 cttcgccgtc accggcgccg aggccgtgac gaacgcggcc acgggcgccg gcgcgcaggt
     1981 gaagatgccg cccacgccca tcccgaccgt tggcacgttc gccgtcatgg ccgacccgca
     2041 gggcgccgtg ttcgcgatcc tccaggcgga gccgcagtaa gcgacagcgg gcctggccgg
     2101 cgtagggggt cacgttcccc ccggtcaccg agggatcctc gcgcgccgtg atcggaggag
     2161 gggagatgat cggagccttg aacgaggccg accgagccgt cgtgccatcg cggaacaggt
     2221 ggaacgaacc gaaccctgaa tccgcgagcg cgcggcgtcg gtaccggacg tcgaacttcg
     2281 cgctgcccgc ggggggcgcc ttccacgcca ccgtgaacgt gtgcacgtgc accgtcgacg
     2341 cgagcggctt cgtgacgatc gcccgcggca tctcgtaggc gccgatctcg caggccggtc
     2401 cctgcgggcg ggggacgccc cgctggtccc gcgcctcgca ccccggcccg ccgtggttga
     2461 gcgccgcgct cgtcggcatg agggccatgg atcgacccgt cgaagctgaa gtgcgagatc
     2521 ccgccgtcgc tcgcgtcccg ggccgtgttc tgggcgatcg tgtcgttgtt gaggtccagc
     2581 gacgagttca cgctctcttg atgaccgtct tcgccgggcc gcggccccgc acggtcacct
     2641 cgtcggtgat gtcgaggtcc cccttcgcgg cctggttctc accccggccg gcgatggtca
     2701 gcgagtaggc cccgccggca cgacgatgac gtccgcgccg ctgcggtcgt tcgccgcaac
     2761 cacggcctcc cgcagcgagc agtcgccgtc acacgctccg tcgtacgtgt ccgccgtctt
     2821 cgtgacctcg aacgggcccg gggcgagggc ctccgcgctc cgcagcggcg tcaccaccag
     2881 ccccagggcg atcacctcgg acaacacgag ggtggatcga aggacacgag acatgacgga
     2941 gcgctccctt acgtccgagg cgggggacgg tacgcgctca cgctccccat ggcagtggag
     3001 cagcttcgat catcgcgtgt gcgcgcagcg acagcgcggt gtgaccggcg cctcaggggg
     3061 ctcgttcacc atccctgccg tgaacctgtg gaagggagtg gagatgagct acgcgatcgt
     3121 tgccgagggg ctggtgaagc ggttcgggga gaccacggct ctggcggggg cggatctggc
     3181 ggccagagcc gggaccgtgc tgggggtgct cggtccgaat ggggccggga agaccacggc
     3241 cgttcggatc ttggccaccc tcacgacgcc ggatagcggg cgggcctcgg tgggcggcta
     3301 cgacgtcgtg gcacaggcgc accaggttcg gcagctcatc gccctcacgg ggcagtacgc
     3361 ggccgtggac gaggggctga ccgggttcga gaacctgctg atgatcgggc ggctgctcgg
     3421 gcggtcgcgg ccggaggcga aggggcgggc cggggacctg ctcgagcgct tcgagctggc
     3481 cgaggcgggc gaccgcccgg tgcggaccta ctcgggcggg atgcggcggc ggctcgatct
     3541 cgccgccagc ctggtcgggc ggcccgccgt cctgtatctg gacgagccga ccaccgg
//
LOCUS       DASPWO010000396         5228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_227565, whole genome shotgun sequence.
ACCESSION   DASPWO010000396 DASPWO010000000
VERSION     DASPWO010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227565"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VEO00_09090"
     CDS             complement(<1..369)
                     /locus_tag="VEO00_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYU58189.1"
                     /translation="MTEKRHPEGREAESAYDLYARGTELLAAGHPHAAATLLSRAKAL
                     EPDKASIREALARALFQTGRLASARREFAKAVSIDPVNDYAHFGLGLACARTGQPTRA
                     MAHLKLAVAMRPEVEHYRAAL"
     gene            432..1613
                     /locus_tag="VEO00_09095"
     CDS             432..1613
                     /locus_tag="VEO00_09095"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HYU58190.1"
                     /translation="MPDLAPFRALRYAAEAGDPASLTSPPYDAITEEQRARHLGASHH
                     NVVRLILGEDRPSDGERENRYTRAAALLRVWRGGGVLALDPEPAVYPYELSFRLDGRD
                     RRVRGLIGAIGLEPFGRGILPHERTMAGPVTDRLRLLREVRANLSPVYAVLQGPCAPQ
                     AALLDRAAARPAVQEAVDEQGVRHRLWVETDVPADLLAAYRDETLLIADGHHRYTTAL
                     AYREEMRERLGPGPWDRMMTLSVDGATEDPPVLPIHRVVSAEPGGSALPAPGVPVASL
                     EAALAACDDDALRIAVAAREDGTVRFRVLDLDGPAPPAVCALHERVLSRYPGAEVHYV
                     PDASAAVAAVEAGADVAYLLPATHVERIRRVIERGERLPQKSTWFWPKPRTGMVIRPL
                     E"
     gene            complement(1688..1999)
                     /locus_tag="VEO00_09100"
     CDS             complement(1688..1999)
                     /locus_tag="VEO00_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HYU58191.1"
                     /translation="MVTVNVVVLAGTLADDPVVRTLPSGDMVTSLRLQVPEPGKRLLP
                     LAVSAWSASTRKAASGLTKGDKVMVAGHIERRFFKGDGGGRAITEVVAREVRQLVPAT
                     G"
     gene            2098..3033
                     /locus_tag="VEO00_09105"
     CDS             2098..3033
                     /locus_tag="VEO00_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYU58192.1"
                     /translation="MATTFAAASGITAIDTRMAGREGLTSAYLLESEEPCLVESGPTT
                     SADAVQEGLSVLGVGPDDLAHIAVTHIHLDHAGGAGELARRYPRATVWVHERGAPHLV
                     DPAKLVASATRVYGEEEMDRLFGPVHPIPADRVRALEGGDVLPLGPRRRLDVVYTPGH
                     AGHQVAFVDSESEALFTGDALGIYLNDVRILRPATPPPEFDLELAVGSIRAIAGREPS
                     RLLFSHFGPADHVEHLCTLAIRRAEKWVGLVREILRETDDLDRVTDLLTEATAAEFNP
                     QGADPGIVERHELLSSVRMNAMGILRYLTRERAKA"
     gene            complement(2918..3679)
                     /locus_tag="VEO00_09110"
     CDS             complement(2918..3679)
                     /locus_tag="VEO00_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYU58193.1"
                     /translation="MARTARGGRGREAAALLERAADHLARDDARGALDAAERAKGLAP
                     RSPTAREVLGIALYRLGRFHDAMRELSTYRRISGRVDQNHLIADCHRALGAPERAVPL
                     AAEALGAAIPAEHRAEAAVVGASALADLGRFAEALALLGRYPEPAGSVRPHDLRVLYV
                     KADVLERAGRRGDAAEAFARIARHDPDAFDVAERLRRLGGALPARAADPNGGSRRVRP
                     SRVRGSGSGGSPSHSSGPTRGVRGAPRSRGRPPAD"
     gene            complement(3870..3986)
                     /gene="rrf"
                     /locus_tag="VEO00_09115"
     rRNA            complement(3870..3986)
                     /gene="rrf"
                     /locus_tag="VEO00_09115"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(4038..>5228)
                     /locus_tag="VEO00_09120"
     rRNA            complement(4038..>5228)
                     /locus_tag="VEO00_09120"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          731 a         1896 c         1865 g          736 t
ORIGIN      
        1 gagggccgcg cggtagtgct cgacctccgg tcgcatggcc accgccagct tcaggtgcgc
       61 catcgcccgg gtcggctgac cggtgcgggc gcaggccagt ccgagcccga agtgggcgta
      121 gtcgttcacc ggatcgatcg agacggcctt cgcgaactcc cgccgcgccg acgcgagcct
      181 gccggtctgg aacagggccc gcgcgagcgc ctcgcggatg gacgccttgt cgggctcgag
      241 ggccttcgcc cgcgacagga gcgtggccgc cgcgtgcggg tggccggccg caagcagctc
      301 ggtgccccgc gcgtacaggt cgtacgcgga ctcggcctcg cggccctcgg ggtggcgctt
      361 ctcggtcacc gggtcaggat acgggcgatc ggctataacc gccctcgtgt ccgaccccgg
      421 gcggtgcgac ggtgcctgac ctcgcgccgt tccgcgcgct gcgttacgcg gcggaggccg
      481 gcgacccggc gtcgctgacc tccccgccgt acgacgccat caccgaggag cagcgcgcgc
      541 ggcacctcgg cgccagccac cacaacgtgg tccggctgat cctcggcgag gaccggccgt
      601 cggacggcga gcgggagaac cgctacacgc gtgccgcggc gctcctgcgc gtctggcgcg
      661 ggggtggggt gctcgccctc gacccggagc ccgccgtcta cccgtacgag ctgtcgttcc
      721 ggctcgacgg gcgcgaccgg cgcgtgcgcg gactgatcgg ggcgatcggt ctggagccgt
      781 tcggccgcgg gatcctgccc cacgagcgga cgatggccgg cccggtcacc gaccggctgc
      841 gcctgctgcg cgaggtgcgg gcgaacctgt cgccggtcta cgcggtgctc cagggcccgt
      901 gtgccccgca ggccgccctc ctcgaccgcg ccgccgcccg cccggcggtg caagaggccg
      961 tcgacgagca gggagtgcgc caccggttgt gggtggagac ggacgtcccg gcggatctcc
     1021 tcgccgcgta ccgcgacgag acgctgctga tcgccgacgg ccaccatcgc tacacgacgg
     1081 ccctcgccta ccgcgaggag atgcgcgagc ggctcggccc gggcccgtgg gaccggatga
     1141 tgaccctctc cgtcgacggg gcgaccgagg accccccggt cctgccgatc caccgggtcg
     1201 tcagcgccga accgggcggc tcggcgctgc ccgcgccggg cgtcccggtc gcctcgctcg
     1261 aggccgcact ggcggcgtgc gacgacgacg ccctgcgcat cgcggtggcg gcgcgcgagg
     1321 acggcacggt caggttccga gtgctcgatc tcgacggccc cgcccccccg gcggtctgcg
     1381 cgctccacga gcgggtgctg tcgcgctacc cgggggccga ggtgcactac gtccccgacg
     1441 cgtcggccgc ggtggccgcg gtggaagcgg gtgccgacgt cgcctacctg ctcccggcca
     1501 cccacgtcga gcggatccgc cgggtgatcg agcgcggcga gcggctgccg cagaagtcga
     1561 cctggttctg gccgaagccg cgcaccggca tggtcatccg cccgctggag tgacggcagc
     1621 ggttcggaac cacgccgagc cggacctcgt attcgacgcg cggcggggag accctgcctc
     1681 ctccggctca gcccgtcgcc gggaccagct gcctgacctc gcgcgcgacg acctcggtga
     1741 tcgccctgcc ccctccatcg cccttgaaga agcgccgctc gatgtggccc gcgaccatca
     1801 ccttgtcacc cttggtgagg ccggaggccg cctttcgcgt ggatgcgctc caagccgaga
     1861 ccgccagcgg gaggagtcgc ttgccgggct cggggacctg gaggcggagg gaggtcacca
     1921 tgtcgccgga cggcagcgtt cgcacgacgg ggtcgtcggc gagcgtgccc gcgagcacga
     1981 ccacgttgac cgtgaccatg gcctcctcct ttcgccgcgg gcaccatagc gcccggtgct
     2041 gacacgcccg ccggcgggcc ggcccgtggc gcgcgcgctc gtcgctagac tcgttccatg
     2101 gcaacgacct tcgcggcggc gagcgggatc acggccatcg acacgcggat ggccgggcgg
     2161 gagggcctga cgtcggccta cctgctggag tcggaggagc cctgcctggt cgagagcggc
     2221 ccgacgacct ccgcggacgc cgtgcaggag ggcctgtccg tgctcggtgt cggcccggac
     2281 gacctggccc acatcgcggt gacgcacatc cacctcgacc acgccggcgg cgcaggcgag
     2341 ctggcccggc gctacccgcg ggccacggtg tgggtccacg agcgaggggc gccgcacctg
     2401 gtggacccgg cgaagctcgt cgccagcgcg acccgcgtgt acggggagga ggagatggat
     2461 cggctgttcg ggcccgtgca cccgatcccg gccgaccggg tgcgtgccct ggagggcggc
     2521 gacgtcctgc cgctcggtcc gcgccggcgg ctcgacgtcg tctacacccc cggacacgcc
     2581 ggccaccagg tggcgttcgt ggactcggag agcgaggccc tgttcaccgg cgacgctctg
     2641 ggcatctacc tgaacgacgt gcgcatcctg cggccggcga ccccgccccc ggagttcgac
     2701 ctcgagctcg ccgtcgggag catccgggcg atcgccgggc gggagccctc gcgcctgctg
     2761 ttcagccact tcggcccggc cgaccatgtc gagcacctct gcacgctggc gatccgccgg
     2821 gcggagaagt gggtcggcct ggttcgcgaa atcctgcgcg agaccgatga cctcgatcgc
     2881 gtgacggatt tgctgacgga agcgacggcc gccgagttca atccgcaggg ggccgacccc
     2941 gggatcgtgg agcgccacga actcctctcg tcggtccgga tgaatgcgat ggggatcctc
     3001 cgctacctga cccgcgaacg cgcgaaggcc tgacccgccg actcccgccg ttcggatccg
     3061 ccgcccgcgc cggcaaggcc ccacccagcc gtcggaggcg ctcggcgaca tcgaaggcgt
     3121 cggggtcgtg gcgggcgatg cgggcgaagg cctctgccgc gtccccccgg cggccggcgc
     3181 gctccaggac gtcggccttg acgtagagga cgcgcaggtc gtgcggacgg acggagccgg
     3241 cgggctccgg gtagcggccg agcagggcca gcgcctccgc gaagcggccg aggtcggcca
     3301 gggcggatgc gccgaccacc gccgcctcgg cgcggtgctc ggccgggatc gcggcgccga
     3361 gcgcctcggc cgccagggga acggcgcgct ccggggcccc gagggcccgg tggcagtcgg
     3421 cgatcaggtg gttctggtcg acgcggccgg agatgcggcg gtaggtcgag agctcgcgca
     3481 tggcgtcgtg gaagcggccc aggcggtaga gcgcgatccc gagcacttcc cgagcggtcg
     3541 gcgagcgcgg ggcgagtccc ttggcccgct ccgcggcgtc gagcgcgccg cgagcgtcgt
     3601 ctcgagcgag gtggtcggcg gcccgctcca ggagcgccgc cgcctcccgg ccccgtccgc
     3661 cgcgcgccgt gcgggccatc tcctcggcca gtgcccgcgg cgccggccgt gccccccgag
     3721 cccggttgcc gccgccggac gccggcgcgt acgcgcccga gccccgtggt gagccgcggc
     3781 gaggtggccg ggggcggcga ggggggggca tggagcaagc gtaggaggaa agacgaagag
     3841 ccgccccaga gggcgactct tcgaagaggt tccggcggcg acctactctc ccaccccgtc
     3901 accagggcag taccatcggc gctggagggc ttaacttccg ggttcggaat gggaccgggt
     3961 gtttcccctc cgccatggcc accgaaacgg tcacgagggc gcgagtcgtc cctcgaaaac
     4021 cgatagcgag cgcgagcaaa gtcaagtcct cggcctatta gtaccggtcg gctcaacacg
     4081 ttgccgtgcg tacacctccg gcctatcaac ctcgtagtct gcgaggggcc ttactccctc
     4141 aaggggatgg gagacctcat ctgggagcga gcttcgcact tagatgcttt cagcgcttat
     4201 ctcttccgaa cgtggccaac cagccgtgcc gctggcgcga caactggcac accagaggtc
     4261 cgtccgtccc ggtcctctcg tactagggac agccctcctc aagtctccaa cgcccacgga
     4321 ggatagggac cgacacatct gttacctccg cccggcacgc acaccgcgcg gcggccaggt
     4381 catttctgcc tggctctgca tgtcgccatg caggtcggac catctcttta ccgccaggtt
     4441 cccgccccca cgggatgggg gagagcggcg gcgtccggca tatggtctct gaggattctg
     4501 gatgcctgcc ttcgatctct tcgtcgatac cgcccaccac cgttcatgga gagagcgatc
     4561 tcgagaagcg aacggaaccc ttcgaagctc agatgctcct tccgacccat cgcccgaacg
     4621 atcggggcga aggggcgagg aactcctgct gcttgctgct cagaaggggc tgcgccacga
     4681 agaagggaac gacccttgtg agaagatcgt tccggttgcg gaccacgagg accaaggtgg
     4741 cgtctcgcga tccgcgatgg ttctccacga tccgtccgca gcccagccgc ccggcgatta
     4801 ggtcgaggac ctgccgccgc tcggcgttct ggctgacgcg gaactccggg acgagttgcc
     4861 aaccggccct cgtcgaggga ttccgctgga cggcgacgtg gaaggatcct tctccgtcga
     4921 cgtatcccgc aatgtaggca tccagccttt cctgctgatt gcccgcaccc gaagcattgt
     4981 cactgcccat gcggagacgc tagcagcagc ctccgacacg gcgagtagct cgggatacac
     5041 ggggttccca gcatttagcc ggatttcagg acagcacgga aagtcctact gtctcacgac
     5101 gttctaaacc cagctcgcgt accgctttaa cgggcgaaca gcccgaccct tgggacctgc
     5161 tccagcccca ggatgcgacg agccgacatc gaggtgccaa accttgccgt cgatatggac
     5221 tcttgggc
//
LOCUS       DASPWO010000397         3987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_315102, whole genome shotgun sequence.
ACCESSION   DASPWO010000397 DASPWO010000000
VERSION     DASPWO010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315102"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VEO00_09125"
     CDS             <1..1159
                     /locus_tag="VEO00_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU58194.1"
                     /translation="VVRDGRAVVSSFDGQPPPDLLGIRAAGDREPVVVRSGETRILDL
                     VEPILGGQLGEIRLGLDLAIVNATRRELAWRLGTLALAVILAGLAAAWLAGRSIARPV
                     NEMLDAADRFDPAAAGAGPAPALRQAGSDEIAVLADRFNRMMRRLESAYAEQARARQK
                     AVETERLAALGSLVAGVAHEVNNPLAGVKNCLRRLERGEVTPAKREEYMALMEEGLDR
                     IEDVVRQLLDFGRPHRAVLEPVSVAALARDAVRLLHPVLEPRRIECAVVAEPGAEGEV
                     RADRREAGQAIVNLLLNAAYVTPDGGRIRVAVRCRGRFLGIAVEDQGPGIPVEVRDRM
                     LDPFFSTKPEGEGTGLGLSVTRTIVDAQGGELTFEFPPEGGTVAAVWLPAA"
     gene            1374..3518
                     /locus_tag="VEO00_09130"
     CDS             1374..3518
                     /locus_tag="VEO00_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58195.1"
                     /translation="MKKLIGIIAAATLLAACSGSDGSIGPPGPVGPIGDTGPIGPPGP
                     PGAGFLPLEVAGVVGYVTDVSDAPVAGATVYFVPQAAVAALAATPINIDPTTTTTAMN
                     AVRTASNDEPLEDLIELNGTTYVSAVTDAGGVYRVTTLPSGEYFVVAVPSDAGHLPGG
                     THCREPLAATTLVGRQLNMQVSSKPGPNAFYVGASVCYTCHGRQHEKYTLHVNGIRPT
                     GKAGALQKGDKYFTRWNEALAKFSAGDATAGGTTLYFTPVGVASTASDWKVSETNPGS
                     GVVLTARLFSVSDGTTTTYNVELKDAINPTGTVYKAEFSYGGGLYKQRYLTKIGTSRY
                     VLPIQYNFDSAPAGGNGINEATDPAGRWNWQHYNLSSNGWFAPGTGLLTVPGKGKAFD
                     NNCAGCHFTGYSLTGNATDGWTAHGVPDANGEADFDGDGQSELMNTTCESCHGPGSDH
                     WAVAGQGKLVVSPRLLTPEREVTICAACHTRALGIGAGATENPLNAGLRMALPGTKRS
                     VWLTEYVSKVADGVWDTKKNNAGGVSTPLGDGLHSRQHHQQASDFMKTRKYRNEFRLV
                     TCTSCHDPHGTADQATAPVEKHQLRAVLDTAPATAGLCLGCHAPFYEAGATVGERMKN
                     HWSANGINANFAKDGIQCATCHTPKTAKSGAGLRQAVLGGQQYWSGDISSHLFRVPRK
                     AVISGKVDGTITGNDVQPIPYTNKCGFCHGAP"
     gene            complement(3638..>3987)
                     /locus_tag="VEO00_09135"
     CDS             complement(3638..>3987)
                     /locus_tag="VEO00_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYU58196.1"
                     /translation="DWPGNVRELVHALERAVALSRGGTIRLEHLPADVAAAAATRGVG
                     AAVISTPAGGEVRPLGEAVGEFEREYIQRVLERTGGHKGRAAALLGISRKSLWERLRE
                     LTSGSGGDRPQKS"
BASE COUNT          589 a         1461 c         1401 g          536 t
ORIGIN      
        1 cgtcgtgcgc gacgggcgcg cggtggtgag cagcttcgac gggcagccac cgcccgacct
       61 cctggggatt cgcgcggcgg gcgaccggga gcccgtggtg gtccgctcgg gcgagacccg
      121 gattctcgac ctcgtcgagc cgattctcgg cgggcagctc ggtgagatcc gactcgggct
      181 cgacctcgcc atcgtcaacg ccacgcgccg cgagctggcg tggcggctcg gcaccctggc
      241 gctcgcggtg atcctggccg gcctcgccgc cgcctggctc gcggggcggt ccatcgcgcg
      301 gccagtgaac gagatgctgg acgcggccga tcgcttcgac cctgcggcgg cgggagccgg
      361 tcccgcgccg gcgttgcgcc aggcaggctc cgacgagatc gcggtgctgg cggatcgctt
      421 caaccgcatg atgcggcgtc tcgagtcggc gtacgccgag caggcgcggg cgcgccagaa
      481 ggcggtggag accgagcggc tcgccgcgct cggctcactc gtcgccggcg tcgcgcacga
      541 ggtgaacaac ccgctcgccg gcgtgaagaa ctgcctgcgc cggctggagc gcggcgaggt
      601 cacgcccgcg aagcgggagg agtacatggc cctgatggag gaggggctcg accggatcga
      661 ggacgtggtg cgccagctgc tcgacttcgg gcgaccgcac cgcgccgtgc tcgagccggt
      721 gtccgtggcg gcgctcgcgc gcgacgcggt ccgcctgctg caccccgtgc tggagccgag
      781 gcggatcgag tgcgcggtcg tcgcggagcc gggcgccgag ggcgaggtcc gcgccgaccg
      841 tcgcgaggcg gggcaggcca tcgtgaacct gctgctcaac gccgcctacg tcaccccgga
      901 tggcgggcgg atccgcgtgg ccgtccggtg tcgtgggagg ttcctcggca tcgcggtgga
      961 ggaccagggt cccggcatcc cggtcgaggt gcgcgaccgg atgctcgacc cgttcttctc
     1021 gacgaagccg gagggcgagg ggaccgggct cggactctcg gtcacccgga cgatcgtcga
     1081 cgcgcagggc ggtgagctga cgttcgagtt cccgcccgag ggcgggacgg tggccgcggt
     1141 ctggctcccg gccgcgtgag cgttccgacc gtcacgcgcg atgttacctg gcggtaacac
     1201 ccgcgcgtcg cgccgcggca ggcttgttac cggtgggtgc cgcgccgatg gtccgatggc
     1261 caacgatctc gaccccttgg cgcatggccg ccgggttgca accggacggc gccgtcgccc
     1321 gcagaacgcc ggcgcggcgc ccatctctcc atcgacggag gcatccgaat cacatgaaga
     1381 aactcatcgg catcatcgcc gcggccacgc tcctcgcagc ctgttccggt tcggacgggt
     1441 ccatcggccc gccgggcccg gtcggtccca tcggcgacac ggggcccatc ggcccccccg
     1501 gccctccggg cgcaggcttc ctgccgctcg aggtggcggg ggtggtcggc tacgtcacgg
     1561 acgtttcgga cgcgccggtg gccggcgcga ccgtgtactt cgttccgcag gccgcggtgg
     1621 ccgcgctcgc cgccacgccg atcaacatcg atcccacgac caccaccacg gcgatgaacg
     1681 cagttcgcac cgcctcgaac gacgagccgc tcgaggacct gatcgagctc aacggcacga
     1741 cgtacgtcag cgcggtcacc gatgccggcg gcgtctaccg cgtcacgacc ttgccgagcg
     1801 gtgagtactt cgtggtcgcg gttccgtcgg atgcgggcca cctgccgggc ggcacccact
     1861 gccgtgagcc gctggccgcg acgacgctcg tcggccggca gctcaacatg caggtgtcgt
     1921 cgaagccggg cccgaacgcg ttctacgtcg gggcctcggt ctgctacacc tgccacggtc
     1981 gccagcacga gaagtacacg ctccacgtga acggcatccg tcccacgggc aaggccgggg
     2041 cgctgcagaa gggcgacaag tacttcacgc gctggaacga ggcgctcgcg aagttctccg
     2101 cgggtgacgc gaccgcgggc ggcacgacgc tctacttcac gccggtcggc gtcgcgagca
     2161 ccgcgagcga ctggaaggtc tccgagacga atccggggtc gggcgtcgtc ctgaccgcgc
     2221 gcctcttcag cgtcagcgac ggcaccacga ccacctacaa cgtggagctc aaggacgcca
     2281 tcaacccgac gggcacggtc tacaaggccg agttcagcta cggcggcggc ctctacaagc
     2341 agcggtacct gacgaagatc ggcacgagcc gctacgtgct cccgatccag tacaacttcg
     2401 actccgcccc tgccggtggg aacgggatca acgaggccac cgaccccgcg ggccgctgga
     2461 actggcagca ctacaacctg tcctcgaacg gctggttcgc tcccggcacc ggcctcctca
     2521 ccgtcccggg caaggggaag gcgttcgaca acaactgcgc gggctgccac ttcaccgggt
     2581 actcgctcac cggcaacgcg acggacggct ggacggccca cggcgttccg gacgcgaacg
     2641 gcgaggccga cttcgacggc gacgggcagt cggagctcat gaacaccacc tgcgagagct
     2701 gccacggccc cggctccgat cactgggccg tcgcgggtca gggcaagctc gtggtctcgc
     2761 ccaggctcct cacgccggag cgcgaggtca ccatttgcgc cgcgtgccac acccgtgccc
     2821 tcggcatcgg cgccggcgcc accgagaacc cgctcaacgc ggggctgcgc atggccctgc
     2881 ctggcaccaa gcggagcgta tggctcaccg agtacgtctc caaggtcgcc gacggcgtct
     2941 gggacacgaa gaagaacaac gctggcggcg tgtcgacgcc gctcggtgac gggctccact
     3001 cgcgccagca ccaccagcag gcttccgact tcatgaagac ccggaagtac cggaacgagt
     3061 tccggctcgt cacctgcacc tcgtgccacg atccgcacgg gaccgccgat caggccaccg
     3121 cgccggtcga gaagcaccag ctcagggcgg tcctcgacac cgcgccggcc accgccggcc
     3181 tctgcctcgg ctgccacgcc ccgttctacg aagcgggcgc gacggtgggt gagcggatga
     3241 agaaccactg gtccgcgaac ggcatcaacg cgaacttcgc gaaggacggg atccagtgcg
     3301 cgacctgcca cacgcccaag acggcgaagt ccggcgctgg cctgcgccag gccgtgctcg
     3361 gcgggcagca gtactggtcg ggcgacatca gctcgcacct cttcagggtg ccccgcaagg
     3421 cggtgatcag cggtaaggtc gacggcacca tcaccggtaa cgacgtgcag ccgattccgt
     3481 acacgaacaa gtgcggcttc tgccacggcg cgccgtagcc gacgacggct cgtagcctga
     3541 aacccggcgc cggtcgcgcg gctctcctgc cgcgcggccg gcgcgtcgtc gtttgacgca
     3601 tccggaagca gcccgcccga cagagccggt ctccggctca ggatttctgc ggccggtcgc
     3661 cgccgctccc ggaggtgagc tcgcgcagcc gctcccacag gctcttgcgc gagatgccga
     3721 gcagcgccgc ggcgcgtccc ttgtgcccgc cggtccgttc cagcacgcgc tggatgtact
     3781 cgcgctcgaa ctcgccgacc gcctcgccga gcggccgcac ctcgccgccg gccggcgtcg
     3841 agatcaccgc ggctccgacg ccgcgcgtcg cggcggcggc ggccacgtcg gccgggaggt
     3901 gctcgaggcg gatcgtccca ccgcgtgaca gcgcgaccgc ccgctcgagc gcgtgcacga
     3961 gctcgcgcac gttgcccggc cagtcgt
//
LOCUS       DASPWO010000398         5533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1242658, whole genome shotgun sequence.
ACCESSION   DASPWO010000398 DASPWO010000000
VERSION     DASPWO010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242658"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VEO00_09140"
     CDS             complement(<1..362)
                     /locus_tag="VEO00_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58197.1"
                     /translation="MERTPGIVRILPGILVAVGGVVTVAGSILPWASMSADIGIPVEE
                     ASTGLALDHGWLTLATGILLIVVGILAIAARAHRAAVAGGLLLSLFLVGFAVYDWVDI
                     AGANGELTEDLRNLVSIS"
     gene            complement(364..1023)
                     /locus_tag="VEO00_09145"
     CDS             complement(364..1023)
                     /locus_tag="VEO00_09145"
                     /inference="COORDINATES: protein motif:HMM:NF021079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HYU58198.1"
                     /translation="MGTEPLQAPPPPAPDTGPAHARARPPLGAGTALAALLVILGGAA
                     GALGSLLTWERVSVDFSLFGREGVREITRTGFETGYGQPAIAAGAVILVAGVLLLLLR
                     GTAIRRALAVVALLAGLAAAGLALYESVQVKGRIVDDNAQGRPAEFRDRLEQAVDVGT
                     EPGRWVVVAGGVIGLVGAAAALIAAARTREPARVAPARATTEFEQPPPGGIPPAPPPP
                     D"
     gene            complement(1030..2616)
                     /gene="trpE"
                     /locus_tag="VEO00_09150"
     CDS             complement(1030..2616)
                     /gene="trpE"
                     /locus_tag="VEO00_09150"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HYU58199.1"
                     /translation="MTSGPATPDRATFAALAGEHAVVPVFREFFADTLTPVAAYRAVR
                     GSGPSFLLESVEHGERWGRFSFIGVAPALTIRARRGRAETEPPELAGEGLLATVEATL
                     AGLTAPQLPGLPPLHSGLVGYVGYDAVREIERLGPPPPDDLGLPESTLIFPTLVVAFD
                     HLRQSLTIVANAIRTEDDAAYADAERRIEELAGRLFDARPDEPRPLLRPRPIPATANV
                     SDEAFAAWVRVAKEHIVAGDIFQVVLSRRFEAPSPPDPFDAYRALRVINPSPYMYFLD
                     FDDVRIAGSSPEVLVKVQGGTALTHPIAGTRPRGATEEEDLRLEEELLADPKERAEHI
                     MLVDLARNDLGRVSSAGSVRVDDLMSVERFSHVMHLTSRVSGTLAPGRTALDVLRAAF
                     PAGTVSGAPKVRAMEIIDDLEPVARGPYAGAVGYLDLGGNLDTCITLRTVVFHGGRAY
                     VQAGAGIVADSDPEAEARETQQKADALLAAIGAADRLAAAGPSPWGARSQSVSGPPAP
                     QGELPSQIPPAGSAPREPEE"
     gene            complement(2613..3404)
                     /locus_tag="VEO00_09155"
     CDS             complement(2613..3404)
                     /locus_tag="VEO00_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HYU58200.1"
                     /translation="MEFEVIPAIDVMGGRLVRLRKGDPASLQRFSGDPLVLARRFVDE
                     GAAWIHFVDLDAAFGGGAANLGLLSALARLPVRVQAGGGLRVEEAEAALAAGAERAVI
                     GANALVDRETVERAVGRHGERVAVALDVRGGRIVPRGSGTPGPPLDLTVRWLSDGGVG
                     AIVYTNVDHDGTLTGPDLDGLRRLAARTDRPVIASGGVASAADVGAVAAIGAPVHGVI
                     VGRALYERGLSVTECRRALGGFEGVARPGEGSARPRPASEPPTAE"
     gene            complement(3434..>5533)
                     /gene="dnaE"
                     /locus_tag="VEO00_09160"
     CDS             complement(3434..>5533)
                     /gene="dnaE"
                     /locus_tag="VEO00_09160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HYU58201.1"
                     /translation="DALELSPELHEAYRSEPESRQIIDTAKALEGLHREDSVHAAGVV
                     VGDAPLVNYLPLKLTKDSRDNERKVVTQFDMKGVELLGLLKMDFLGLRTLSVIEDTVA
                     FLRRRGVDLDIDHLSLHDEPTYRMLQDADTTGVFQLEGGGMRSLIRQLLPSRFEDLMA
                     LVALYRPGPLNVGSHIDYAERKHGRREVTYPHADLEPVLRDTYGVIVYQEQVMETAVR
                     LAGYTMGEADTLRKAMGKKIRADLIPHRAKFVRQCVERGFEEKLAQDIFDLITPFADY
                     GFNASHACGYALIAYQTAYLKRHHPVEYFAALLTSVKDDRDRKPFYLNAARLHGIRVL
                     PPDVNGSEIDFTPVGGREGFAADGDEIRYGLSAVRGIGAGAVGQIVEERRARGPFASF
                     PAFCRRVDPQILNKKTIESLILSGAFDSLGYARRALFEGFEKVVGPILSDRRAEAAGQ
                     FSLFGGAEEDRSKAIDESVLQGPEFEKRELLGREKEVLGQFVTDHPLLEVRDRLARQT
                     ECEIGDLVDKGDGDVLLVGGIVGAVGRKYTRKGEPYVLFRLEDLAGGVGVIAFPSVFD
                     RDPTLFTPDTIVLVKGRIDLRGRELQLVALEVTRPDLSSDDVAAPAPGDPVVVTMPAE
                     LCTSGVVGKLKQVLADHPGSVPVLVRLTAGDGPTRTLRIGNGYRVDGSEALLAELRTL
                     LGPAAAARELAGAAAGA"
BASE COUNT          766 a         2062 c         1980 g          725 t
ORIGIN      
        1 atcgagatcg agaccaggtt cctcaggtcc tcggtcagct ccccgttggc gccggcgatg
       61 tcgacccagt cgtagacggc gaacccaacc aggaacaggg agagcagcag gccgccggcc
      121 accgccgccc ggtgagcccg agcggcgatc gccaggatgc cgacgacgat cagcaggatc
      181 cccgtcgcga gcgtgagcca gccgtggtcc agcgcgaggc ccgtcgacgc ctcctccacc
      241 gggatcccga tgtccgccga catcgacgcc cacggcagga tggatcccgc cacggtcacc
      301 accccgccga ccgccacaag gattcccggc aggatccgaa cgatccccgg cgttcgttcc
      361 acgtcagtcg ggaggtggcg gagccggggg gatccctccg ggagggggtt gttcgaactc
      421 ggtggtcgcg cgggccgggg cgacgcgggc gggttcgcgg gtgcgggcgg ccgcgatcag
      481 ggcggcggcg gcgccgacaa ggccgatgac gcccccggcg acgacgaccc accggccggg
      541 ctccgtgccg acgtcgacgg cctgttccag ccggtcgcgg aactcggccg ggcgcccctg
      601 cgcgttgtcg tcgacgatcc gccccttgac ctggacgctc tcgtagagcg ccaggccggc
      661 cgccgccagg ccggccagca gggcgaccac cgcgagcgcg cgccggatcg ccgtgccgcg
      721 gagcaggagc aggagcacgc cggccaccag gatgacggca ccggcggcga tggccggctg
      781 gccgtacccg gtctcgaacc cggtccgggt gatctcgcgc acgccctccc gtccgaacag
      841 ggagaagtcg accgacaccc gctcccaggt cagcaacgac ccgagcgccc ccgccgcccc
      901 tcccagaatc accagcaggg cggccagcgc ggtgcccgcc ccgagcggag gccgggcccg
      961 cgcgtgggcc ggcccggtgt cgggggccgg cgggggcggg gcctgaagcg gctcggttcc
     1021 catggcggcc tattcttccg gttcccgcgg tgcgctgccc gcggggggga tctgggacgg
     1081 tagctcgccc tgtggcgcgg gcgggccgga caccgactgc gagcgagccc cccagggaga
     1141 agggccggcc gccgccaggc ggtcggcggc gccgatcgcg gcgaggagcg cgtcggcctt
     1201 ctgctgcgtc tcccgcgcct cggcctccgg atccgagtcg gcgacgatgc cggcgccggc
     1261 ctgcacgtac gcccggccac cgtggaagac cacggtgcga agcgtgatgc acgtgtcgag
     1321 gttgccgccg aggtcgaggt acccgaccgc cccggcgtac gggccgcggg ccaccggctc
     1381 gaggtcgtcg atgatctcca tcgcccggac cttcggcgcc cccgaaacgg ttcccgccgg
     1441 gaacgccgcg cggagcacgt cgagcgcggt ccggcccggc gccagggtgc cggagacccg
     1501 cgaggtcagg tgcatgacgt gcgagaagcg ctccaccgac atcaggtcgt ccacgcgcac
     1561 gctccccgcg ctgctgaccc ggccgaggtc gttgcgcgcc aggtccacga gcatgatgtg
     1621 ctcggcgcgc tccttgggat ctgcaagcag ttcctcctcg agccggaggt cctcctcttc
     1681 ggtcgcccct cgcgggcgcg tcccggcgat cgggtgggtc agcgccgtcc cgccctgcac
     1741 cttgaccagg acctcgggcg atgagccggc gatccggacg tcgtcgaagt ccaagaagta
     1801 catgtacggc gaggggttga tcacgcgcag cgcccggtac gcgtcgaacg ggtccggcgg
     1861 gctcggggcc tcgaaccgcc gcgacagcac cacctggaag atgtccccgg cgacgatgtg
     1921 ttccttcgcc accctgaccc acgccgcgaa ggcttcgtcg gagacgttgg ccgtggcggg
     1981 gatcgggcgc gggcggagca gcggccgggg ctcgtcgggc cgggcgtcga acagtcgccc
     2041 ggccagctcc tcgatccgcc tctcggcgtc ggcgtatgcc gcatcgtctt ccgtccggat
     2101 ggcgttcgcc acgatcgtca gcgactggcg caggtggtcg aacgcgacca cgagcgtcgg
     2161 gaagatgagg gtggactccg ggagcccgag gtcgtcgggg ggcggggggc cgagacgctc
     2221 gatctcgcga acggcgtcgt agcccacata gccgaccaat cccgagtgca gcggcgggag
     2281 cccggggagc tgcggtgccg tcagcccggc gagcgtcgcc tcgacggtcg ccagcagccc
     2341 ctcgccggcc agttcggggg gttcggtctc ggctcgaccg cgacgggcgc ggatcgtcag
     2401 cgccggggcg acgccgatga acgagaaccg tccccagcgc tcaccgtgct cgacgctctc
     2461 gagcaggaac gacggtccgg acccgcgcac ggcccggtag gcggccaccg gggtcagggt
     2521 gtcggcgaag aactcccgga acacggggac gacggcgtgc tcgccggcca gcgccgcgaa
     2581 cgtggcacgg tcgggcgtgg ccggcccgct cgtcattctg ccgtgggggg ctcgctcgcc
     2641 ggacgtggtc gggctgatcc ctctccgggg cgagccaccc cctcaaaccc cccgagcgcc
     2701 cggcggcact cggtcacgct caggccgcgc tcgtagagcg cccgaccaac gatcacgccg
     2761 tgcaccggcg cgccgatcgc cgcgaccgcg cccacgtcgg cggccgaggc caccccgccg
     2821 gaggcgatga ccggccgatc ggtccgtgcg gccagccggc gcaggccgtc cagatccggc
     2881 ccggtgagcg tcccgtcgtg gtcgacgttc gtgtagacga tggccccgac cccgccgtcg
     2941 ctcagccagc ggacggtgag gtccagcggg gggccgggcg ttcccgatcc gcgcgggacg
     3001 atccggcctc cgcgcacgtc cagggcgacc gcgacgcgct ccccgtgccg gccgacggcc
     3061 cgctcgaccg tctcccggtc gacgagcgcg ttcgccccga tcaccgcccg ttcggcgccg
     3121 gccgcgaggg ccgcctcggc ctcctcgacg cgcaggccgc ctcccgcctg gacccggacc
     3181 ggcagtcggg ccagcgccga cagcagaccg aggttcgccg cgcccccgcc gaaggccgcg
     3241 tcgaggtcca cgaagtggat ccaggcggca ccctcgtcga cgaaccgccg cgccaggacc
     3301 agcgggtcgc cggagaatcg ctgcagggaa gcggggtcgc ccttgcgcag gcgcaccagg
     3361 cgcccgccca tgacgtcgat cgccgggatg acctcgaact ccacgaggcc cggagcctac
     3421 cggagcgccg ggactacgcc ccggccgccg cgccggccag ctcccgggcg gccgcggccg
     3481 ggccgaggag ggttcgcagc tcggcgagca gcgcctccga gccgtcgacc cgatagccgt
     3541 tcccgatccg cagcgtccgg gtcggcccgt ccccggcggt cagccgcacc agcacgggca
     3601 ccgagcccgg gtggtcggcc aggacctgct tcagcttccc gaccaccccg ctggtgcaca
     3661 gctcggcggg catcgtcacc accaccgggt cgccgggcgc cggcgcggcc acgtcgtcgg
     3721 aggacaggtc cggccgcgtt acctccagcg cgacgagctg cagctcgcgc ccgcgcaggt
     3781 cgatccgccc cttgacgagc acgatggtgt cgggggtgaa cagcgtcggg tcgcggtcga
     3841 acaccgaggg gaaggcgatc acgccgaccc cccccgccag gtcctccagg cggaacagga
     3901 cgtagggctc gcccttgcgc gtgtactttc gcccgacggc cccgacgatc ccaccgacga
     3961 ggaggacgtc cccgtctccc ttgtcgacca ggtcgccgat ctcgcactcg gtctggcggg
     4021 ccagccggtc gcgcacctcg agcagcgggt ggtcggtgac gaactgcccg aggacctcct
     4081 tctcccggcc gagcaactcg cgcttctcga actccgggcc ctgcagcacc gactcgtcga
     4141 tcgccttcga gcggtcctcc tcggcgccgc cgaacagcga gaactgcccc gccgcctccg
     4201 cccggcggtc cgacaggatc gggccgacca ccttctcgaa gccctcgaac agcgcccgtc
     4261 gcgcgtaccc cagcgagtcg aacgcgccgg agaggatcag gctctcgatc gtcttcttgt
     4321 tcaggatctg cgggtccaca cggcggcaga atgccgggaa cgaggcgaac gggcccctcg
     4381 cccggcgctc ctcgacgatc tggccgaccg cgccggcccc gatcccgcgc acggccgaca
     4441 gcccgtagcg gatctcgtcg ccgtccgcag cgaacccctc acggcccccg acgggcgtga
     4501 aatcgatctc ggagccgttc acgtcgggcg ggagcacccg gatgccgtgc agtcgcgcgg
     4561 cgttcaggta gaacggcttg cggtccctgt cgtccttcac gctcgtcagc agggcggcga
     4621 agtactccac cgggtggtgc cgcttcaggt aggcggtctg gtaggcgatc agcgcatacc
     4681 cgcaggcgtg gctggcgttg aacccgtagt cagcgaacgg ggtgatcagg tcgaagatgt
     4741 cctgtgccag cttctcctcg aatccgcgct cgacgcactg gcggacgaac ttcgcgcggt
     4801 gggggatcag gtcggcccgg atcttcttgc ccattgcctt gcgcagggtg tcggcctcgc
     4861 ccattgtgta gccggccagg cgcacggcgg tctccatgac ctgctcctgg tagacgatca
     4921 ccccgtaggt gtcccgcagc accggctcga ggtcggcatg cgggtaggtc acctcgcgcc
     4981 tcccatgctt gcgctcggcg tagtcgatgt ggctcccgac gttcagcggt ccggggcggt
     5041 agagggcgac gagcgccatg aggtcctcga agcggctcgg cagcagctgg cggatcagcg
     5101 agcgcatccc gccgccctcg agctggaaca ccccggtcgt gtcggcgtcc tgcagcatcc
     5161 ggtaggtcgg ctcgtcgtgg agcgacaggt ggtcgatgtc gagatcgacg ccgcgacggc
     5221 gcaggaaggc cacggtgtcc tcgatcaccg aaagcgtgcg cagcccgagg aagtccatct
     5281 tcagcaggcc gagcagctcg acgcccttca tgtcgaactg cgtgacgact ttgcgttcgt
     5341 tgtcccgcga gtccttcgtc agcttcagtg ggaggtagtt gacgaggggc gcgtcgccga
     5401 ccaccacgcc cgcggcgtgc accgagtcct cgcggtgcag tccctccagc gccttggcgg
     5461 tgtcgatgat ctgccgagac tccggctcgc tgcggtacgc ctcgtggagc tccggggaca
     5521 gctcgagcgc gtc
//
LOCUS       DASPWO010000399         3406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_479676, whole genome shotgun sequence.
ACCESSION   DASPWO010000399 DASPWO010000000
VERSION     DASPWO010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479676"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1283)
                     /locus_tag="VEO00_09165"
     CDS             complement(138..1283)
                     /locus_tag="VEO00_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58202.1"
                     /translation="MAARWIRAGVAAGIVAAVLPAGPAGASFPGSNGRIAFVSERSGN
                     LDIWSMNPNGSGLLNLTHNAALDEHPAWNPAGTRVAFDSDRSGRRHVYVMNADGSGVV
                     DLTPNSSFDYWPAWKPDGTKILFTRASDIWSMNPDGSGATNLTKSTGTSERDAEWSPN
                     GSKIAYTRYEGNWELYVMNADGSAQTRLTTNDEYDFEPNWAPDGSKLVWRSGMGDNVG
                     EIWIMNPNGTGMVDLTNDPAYDREPTWAPDQTRVAYASVRGTNFEIMAMAPDGSGPVN
                     LTNHASNDYSPDWGSAPATPPPPGCTITGTDRNDVLVGTPGDDVLCGRGGSDSLDGRG
                     GADILRGGPGGDSLEARDGVSGNDALNGGTGTDRCRADPGDTKTACP"
     gene            complement(1324..2679)
                     /locus_tag="VEO00_09170"
     CDS             complement(1324..2679)
                     /locus_tag="VEO00_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU58203.1"
                     /translation="MEAPEVRYAKSGDVNVAYGVVGDGPFDLVFVSGWVLSALEVAWD
                     GPAAETVSRLASFSRLIVFDKRGTGLSDRDTGIPDLETRMDDIRAVMDAVGSRRAAIM
                     GVSEGGPMTLLFAATYPERTAAAVLYGTSASNVKADDYPWGLTREEGLERIRQRAANI
                     GTEAWLDERLRVFAPSHADDEAAKRWWRRWVRTSASPASILALWRMNLEIDARHTLSS
                     ISVPTLVLHPVGDEVTYFDEGRYIADRIPGAELVELPGHDHAWWYRPEEIGREVERFL
                     RGIWDRGEWDVVEHDRVLATVMFTDIVDSTAKLAELGDRGWRDLLQHHHALVRRQLVR
                     YSGREIDTAGDGFFASFDGPARAIRAARAITESVRELGIEVRAGLHTGECELVDGKAG
                     GIAVHIGARVASHAGPSEVLVSSTVKDLVAGSGIQFDPRGTVELKGVPGAWSLYAVTT
                     A"
     gene            2756..>3406
                     /locus_tag="VEO00_09175"
     CDS             2756..>3406
                     /locus_tag="VEO00_09175"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HYU58204.1"
                     /translation="MEPPRRTGYGRGVRVAWPLAVVVGLFGIPFGILARDAGMGALAP
                     VLMSATTFAGSAQFAAVSVLGSSGALAAAVIAAALLNARYVPIGVSVAQDLPGSWPKR
                     LLLGQLVVDESWAVSARGDGRFDRHVLVAAGLLLYMAWVGGTALGVLGATVLPDPETL
                     GLDGAFPALFLSLLAPHVRSRPALAAAVGGAAIALFLIPLTPAGVPIVAASVMAILG"
BASE COUNT          418 a         1260 c         1199 g          529 t
ORIGIN      
        1 gctcccaggt cacaccctcc tggccttcga tcatcaggca gacgcgcacg gcgtcagtct
       61 agccacgggg ggctccgccc cccgtgcacc ccccgacggc caggagcgcc tcgagcgcgc
      121 cgatcgcccg gcctgcccta cgggcaggcc gtcttcgtgt cgccggggtc ggcccggcag
      181 cgatcggtgc cggtcccgcc gttcagcgcg tcgttcccgg agaccccgtc gcgcgcctcg
      241 agggagtcgc cgcccggccc gccgcgcagg atgtcggctc ctcccctgcc gtcgagggag
      301 tcgctgccgc cacgtccgca gagcacgtcg tcacccggcg tgccaacaag gacgtcgttc
      361 cggtcggtgc cggtgatcgt gcatccgggc gggggcgggg tcgcgggcgc ggagccccag
      421 tcgggcgagt agtcgttcga cgcgtggttg gtcaggttca cggggcccga gccgtcgggc
      481 gccatggcca tgatctcgaa gttcgtcccg cgcacgctcg cgtacgcgac cctcgtctgg
      541 tcgggcgccc acgtcggctc gcggtcgtag gcggggtcgt tcgtcaggtc gaccatcccg
      601 gtgccgttcg ggttcatgat ccagatctcg ccgacgttgt cgcccatccc gctgcgccag
      661 acgagcttcg aaccgtccgg cgcccagttc ggctcgaagt cgtactcgtc gttcgtcgtc
      721 aaccgggtct gtgcggaacc gtccgcgttc atcacgtaca gctcccagtt cccctcgtag
      781 cgggtgtagg cgatcttgga cccgttcggc gaccactccg cgtcgcgctc gctggtaccc
      841 gtgctcttcg tcaggttcgt ggcgccgctg ccatcggggt tcatcgacca gatgtccgag
      901 gcgcgcgtga agaggatctt cgtgccgtcg ggcttccacg ccggccagta gtcgaacgac
      961 gagttcggcg tgaggtcgac gacgccggac ccgtcggcgt tcatcacgta gacgtgccgc
     1021 cgccccgagc ggtcgctgtc gaacgcgacc ctggtgccgg cgggattcca ggcggggtgc
     1081 tcgtcgagcg ccgcgttgtg ggtgaggttc aggaggccgg agccgttcgg gttcatcgac
     1141 cagatgtcga ggttgcccga ccgctcactg acgaacgcga tccggccgtt cgagcccggg
     1201 aacgacgccc ccgcgggacc ggcgggcagc acggcggcca cgatccccgc cgccaccccc
     1261 gcgcgaatcc accgcgccgc catcaccggc aatacgcacc ccggacgcca ggggattccc
     1321 acctcaggcg gtggtgaccg cgtagagaga ccaggcgccc ggcacgccct tcagctcgac
     1381 ggtgccgcgc gggtcgaact ggatgccgga gccggctacg aggtccttca ccgtgctcga
     1441 gacgaggacc tcgctcgggc cggcgtggga ggccacgcgc gcgccgatgt gcacggcgat
     1501 gccgccggcc ttgccgtcca ccagttcgca ctcgccggtg tgcaacccgg cgcgcacctc
     1561 gatccccagc tcgcggaccg actcggtgat cgcccgcgcc gcccggatcg ccctggccgg
     1621 cccgtcgaag ctggcgaaga acccgtcgcc ggccgtgtcg atctcgcggc cggagtaccg
     1681 cacgagctgg cggcggacca gcgcgtgatg atgctgcagc aggtcgcgcc agccccggtc
     1741 ccccagctct gccagcttcg ccgtcgaatc gacgatgtcg gtgaacatga ccgtcgccag
     1801 gacacggtcg tgctcgacca cgtcccactc gccgcggtcc cagatgccgc gcaggaagcg
     1861 ctcgacctcc cggccgatct cctccggtcg gtaccaccag gcgtggtcgt ggcccggcag
     1921 ctccaccagc tcggcgccgg ggatgcggtc cgcgatgtag cgcccctcgt cgaagtacgt
     1981 cacctcgtcg cccaccgggt ggaggacgag cgtgggcacg gagatggatg acagggtgtg
     2041 ccgcgcatcg atctccagat tcatccgcca cagcgcgagg atcgacgcgg gactggcgct
     2101 ggtgcgcacc catcgccgcc accatcgctt cgccgcctcg tcatccgcgt gactcggcgc
     2161 gaagacccgc agccgctcgt ccagccacgc ctcggtcccg atgttcgcgg cgcgctgccg
     2221 gatgcgctcc aggccttcct cgcgggtcag gccccatggg tagtcgtcgg ccttcacgtt
     2281 ggacgcgctg gtgccgtaca ggaccgccgc cgcggttctc tccgggtagg tggccgcgaa
     2341 cagcagcgtc atcggcccgc cctcggagac ccccatgatc gcggcgcggc gcgaccccac
     2401 cgcgtccatc acggcccgga tgtcgtccat ccgcgtctcg aggtccggga tcccggtgtc
     2461 gcgatcggac aggccggtcc cgcgcttgtc gaagacgatc aagcgcgaga acgaggccag
     2521 ccgggagact gtctcggccg ccggcccgtc ccaggcgacc tcgagcgcgg agagcaccca
     2581 cccgctgacg aagacgaggt cgaacggccc gtccccgacc acgccgtacg cgacgttgac
     2641 gtccccgctc tttgcgtacc gaacctccgg cgcctccatc gatcagagtg tgtcccccgg
     2701 ctgccatccc ggcaagtgac cgggccgttt ccggcgtttt cggcaagatg gggcgatgga
     2761 accgccgcgc cgaaccgggt acggacgcgg ggtccgcgtg gcgtggccgc tcgcggtggt
     2821 ggtgggcctg ttcgggatcc cgttcggcat cctggcccgc gatgccggga tgggcgcgct
     2881 ggcgccggtc ctgatgtcgg ccaccacgtt cgccggctcg gcgcagttcg cggcggtgtc
     2941 ggtgctcggc tcgagcgggg cgctggcggc ggccgtgatc gcggccgcgc tcctgaacgc
     3001 gcggtacgtg ccgatcgggg tgtcggtggc gcaggacctc ccgggctcgt ggccgaagcg
     3061 gttactgctc ggccagctgg tggtcgacga gtcgtgggcg gtgtccgccc ggggcgacgg
     3121 ccgcttcgac cgccacgtcc tggtggcggc ggggctgctc ctctacatgg cctgggtggg
     3181 cgggaccgcg ctcggcgtgc tcggcgctac ggtcctcccc gacccggaga cgctggggct
     3241 cgacggcgcc ttcccggcgc tgttcctgtc gctgctagcg ccgcacgtcc ggtcgcggcc
     3301 ggcgctggcg gcggccgtcg gcggggcggc catcgcgctg ttcctgatcc cgctcacgcc
     3361 cgccggcgtc ccgatcgtgg cggccagcgt gatggcgatc ctcggg
//
LOCUS       DASPWO010000400         6448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_907157, whole genome shotgun sequence.
ACCESSION   DASPWO010000400 DASPWO010000000
VERSION     DASPWO010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907157"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VEO00_09180"
     CDS             <1..392
                     /locus_tag="VEO00_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYU58205.1"
                     /translation="RGEESRWGAFLDSVMDRVCDALLLGALYWTLRGEGLDLPAALAL
                     AGLAISLLVSHIRAEAEGHGVTMTEGFFQRLDRYVALILGLTIPGALLPALSILVGLG
                     ALTVLQRTYSGWRGLGQEKSDKSVMGT"
     gene            574..1227
                     /locus_tag="VEO00_09185"
     CDS             574..1227
                     /locus_tag="VEO00_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58206.1"
                     /translation="MRRFGRVLAPLGVSAVLLASCAGSEFTYHKSPDNRAFFRVPTDW
                     TMFQKRELLVASGQSLSTATQERFPWMIGFDADEKPQVGRVLNIAGAPDAPVVIAQVQ
                     RLTFSQHDVISLGGLRNVVYPVDQLLDADAAEILDYQELVLDGGLRGIRIVFDVLPSG
                     IANVQFGNDVIRVNQTAILDPGTELLYLLVVRCESHCYRDNARAIKQIVDSWTVKER"
     gene            1228..3870
                     /locus_tag="VEO00_09190"
     CDS             1228..3870
                     /locus_tag="VEO00_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020511792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYU58207.1"
                     /translation="MADDKPGLAWPDHDRGPTPPPPHVSRERDEERATRKPLEWWDRV
                     RIILLFVGAFLVLFWAALAQYDPQISGAEAFSQTLRSYWWLLALAGLELIRQIHYLIS
                     EHVGAYHSFWTKRVFGRGARADGRRRTSDWTRYRISRASKLLFFLLILDLVVAKIYSL
                     PAATALIQLPVIIFQALPFIMQLAFGFFFVMIQFIGLFWFLSRGGIDVYMPDDIKTRF
                     TDVWGQDQVLARIRENMVFLEDPESIERKGGYVPGGILLWGPPGTGKTLMAEAAAGET
                     ERPFVFVDPGAFINMFMGVGILKVKSLFRKLRKLAVRYGGVIVFFDEADSLGSRGALM
                     PGGPWGGQMTGDPFGPHPACNGLAYLAPETASTLFRAALAPSSAEEPRGRVRDRIFFG
                     GMGGGGGGGMGTLPALLTELSGLKKPRGFFNRVLRRALGLRPKPPPKYRILVMMATNL
                     PQALDEALLRPGRIDRIYKVGYPSKAGRIRTYEGYLDKVKHALSAEEIDKLATITPYA
                     TGATIKDLVNEALINAIRGGRDVIEWQDIIKAKQLKDLGPPEDVEYIERERHAVAVHE
                     ACHAVAAFRVRQHLTIDIATIEKGGTYLGMVASIPPEDQFTRWRSEYEADIMVSLSSL
                     AGERLFFEGDSSSGVSGDLEGATKLATLMEGYWGMGETVASHGVTHEVGIGGGGKPGD
                     GKKGKEKDLLESGLGGRIEKNLESLLARAETLLRENRREVLAVAHALETNKTVTGDDV
                     EAIIEGRPGLLIDGSVYASDDFLALAEDYHTAVVSAHKTHDKVSVPLPVLNGQRRALE
                     PGELVAAGGSSPDGPSGNGPGAADPPAPPAPGLAAPPPPGLPSVVHDTTVLVEGACPN
                     CGRVNAEWARFCTRCGTELRPPQP"
     gene            3891..>6448
                     /locus_tag="VEO00_09195"
     CDS             3891..>6448
                     /locus_tag="VEO00_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU58208.1"
                     /translation="MSCPNCGAENAPGARFCSRCGASLARRCANCGADLAEGARFCSA
                     CGTPVEPEAAELAEERKLVTILFADVTGSTQLGERLDPERMKEVMSRFFAAMRAEIEA
                     EGGTVEKFIGDAIMAAFGVPAAHEDDPSRALRAVMRMRTSLDALNRDLEREHGVSLAV
                     RTGVNTGEVIAVTDPRPGEALATGDAVNVAARLEQAAEPGQVLVAERTARAARGFRFR
                     DLGPVSVKGKEEPVRALELLGTAEGAEGAQRGIPGLRAPMVGRDEELSLLRTVYRRLA
                     AEGQPNLATIYGDPGVGKSRLAREFAAWVEVQEPRPTVLAGRCLPYGEGVTYWPLAEI
                     LKSHAGVLDSDPPDRALARIRAATEALLATEAAADPARTAAALAFAMGLEDPAFRLAD
                     LAPRQVRLETHNAWRAFFSEIARPAPAVVVVEDIHWADPALLDLLEDLADRALGPVLF
                     LCPARPELTGRRAGWGGGRRNFTSIFLEPLSHEDADRLVGFLLAIEDLPESVHERILA
                     RAEGNPFFLEEILRRLIDEGRIVREGDRWRASAAIGEVEIPDTVQAVLAARIDLLASE
                     EKRALQSAAVVGRVFWSGPVGELLNGEGERLEEILQRLEDRELVSARLGSSLAGEREY
                     IFKHVLTRDVAYESLPRRERAVAHAEVASWIERTAGERRSEFAELLAYHYSEAHRSAG
                     ATGDREGLRRKAFEYLVAAARGARAKFSVATAVRLGEEAASLADGPLERSRALEVLGL
                     TYIDDYRGFPAYDTLRAAVKERIAGDPTDHLGIAMLCARAIETPTRWPGAMQGLPPAA
                     EAASYLATGLEHAGTADSEARLRLLTARAFWPFAYPDDTPPEEWQEAERAGEEA"
BASE COUNT          900 a         2286 c         2331 g          931 t
ORIGIN      
        1 tgcgcggcga ggagagccgc tggggggcct tcctcgactc ggtgatggac cgcgtctgcg
       61 acgctctgtt gttgggcgcg ctctactgga cgctccgcgg ggagggcctc gacctgccgg
      121 ccgcgctggc cctcgctggc ctggcgatct cgctcctggt cagccacatc cgcgccgagg
      181 cggagggcca cggcgtgacg atgaccgagg ggttcttcca acgcctcgac cgctacgtcg
      241 cgctgatcct cgggctcacg atccccggag cgctgctccc ggcgctctcg atcctggtgg
      301 ggctcggcgc gctcaccgtc ctgcagcgca cgtactcggg ctggcgcggc ctggggcagg
      361 aaaagagtga caaaagcgtg atgggaactt gaccgatccc gcggggatcg cgtagcgtgc
      421 gcgatggaac cgctggagtc ttcggacttc ggcggttccg tttttccccc gtcgtccgcg
      481 cctggcggca gaccgcctcc cgggttcccc gccccccgag cggtacccgc ttcggcccgt
      541 ccgggcctat gatcgtgtgc aaccggatcc cccatgcgca ggttcggcag ggttctcgca
      601 cccctcgggg tttcagcggt cctcctcgcc tcctgcgccg gctcggagtt cacctaccac
      661 aagagccccg acaaccgcgc cttcttccgc gtaccgacgg actggacgat gttccagaag
      721 cgggagctgc tggtggcctc gggacagagc ctctcgaccg ccacgcagga gcggttcccg
      781 tggatgatcg gcttcgacgc cgacgagaag cctcaggtcg gccgggtcct gaacatcgcg
      841 ggcgcccccg atgctccggt cgtcatcgcc caggtccaac gcctcacgtt cagccagcac
      901 gacgtcatct cgctcggcgg cctgcgcaac gtcgtctatc ccgtggatca gttgctggac
      961 gcggacgcgg ccgagatcct cgactaccag gagctggtgc tcgacggtgg ccttcgcggc
     1021 atccggatcg tcttcgacgt cctgccttcc ggcatcgcga acgtgcagtt cggcaacgac
     1081 gtgatccggg tgaaccagac cgccatcctc gacccgggta ccgagctcct ctacctgctc
     1141 gtcgtccgct gcgagtcgca ctgctaccgc gacaacgccc gtgctatcaa gcagatcgtc
     1201 gattcctgga ccgtgaagga gcgctgagtg gctgacgaca agccgggtct ggcctggccc
     1261 gaccacgatc gcgggccgac gccccctccc ccgcacgtga gccgggaacg ggacgaggag
     1321 cgcgccaccc gcaagccgct cgaatggtgg gatcgcgtcc ggatcatcct gctgttcgtc
     1381 ggcgcgttcc tggtcctgtt ctgggcggcc ctcgcgcagt acgacccgca gatctccgga
     1441 gcggaggcat tctcgcagac gttgcggtcc tactggtggc tgctggcgct ggccggcctg
     1501 gaactgatcc gccagatcca ttacctgatc agtgagcacg tcggcgccta ccacagcttc
     1561 tggacgaagc gcgtgttcgg acgcggagcg cgtgcggacg gccggcgtcg aacgagcgac
     1621 tggacgcgct atcgcatctc gcgcgcgtcg aagctgctct tcttcctgct gatcctggac
     1681 ctggtcgtcg cgaagatcta ttcgctgccg gcggcgacgg cgctgatcca gcttccggtg
     1741 atcatcttcc aggcgctgcc gttcatcatg cagctcgcct tcggcttctt cttcgtgatg
     1801 atccagttca tcgggctgtt ctggttcctg tcacggggcg gcatcgacgt ctacatgccc
     1861 gacgacatca agacccgctt caccgacgtc tgggggcagg accaggtcct ggcgaggatc
     1921 cgcgagaaca tggtgttcct cgaggatccc gagtcgatcg agcggaaggg tggctacgtc
     1981 cccggtggca tcctgctctg gggcccgccc ggcaccggca agaccctgat ggccgaggcc
     2041 gcggccgggg agacggagcg gccgttcgtc ttcgtcgacc ccggcgcctt catcaacatg
     2101 ttcatgggcg tcggcatcct gaaggtgaag tcgctcttcc ggaagctgcg caagctggcc
     2161 gtgcggtacg gcggcgtgat cgtcttcttc gacgaggccg actcgctcgg cagccgcggc
     2221 gcgctgatgc cgggcgggcc gtggggcggg cagatgaccg gcgacccgtt cggtccgcac
     2281 ccggcctgca acggcctcgc ctacctcgcg cccgagacgg cgtcgacgct cttccgggcc
     2341 gcgctcgccc cgtcgagcgc cgaggagccg cgcggacggg tccgggaccg gatcttcttc
     2401 ggcggcatgg gcggaggagg cggcggcggg atgggaacgc ttcccgcgct gctcacggag
     2461 ctgtcagggc tgaagaagcc ccgcgggttc ttcaaccggg tcctgcgccg tgcgctggga
     2521 ctgcggccga agcccccgcc gaagtaccgg atcctggtga tgatggccac caacctgccg
     2581 caggcgctgg acgaggccct gctgcgtccg ggtcggatcg accggatcta caaggtcggc
     2641 tacccgtcga aggccggacg gatccgcacg tacgagggct acctggacaa ggtcaagcac
     2701 gccctgtccg ccgaggagat cgacaagctc gccacgatca ccccgtacgc caccggcgcc
     2761 accatcaagg acctggtgaa cgaggcgctg atcaacgcga tccgcggcgg gcgcgacgtg
     2821 atcgagtggc aggacatcat caaggcgaag cagctgaagg acctcggccc cccggaggac
     2881 gtcgagtaca tcgagcgcga gcgccacgcg gtggccgtgc acgaggcctg ccacgcggtg
     2941 gccgccttcc gcgtgcgcca gcacctgacg atcgacatcg cgacgatcga gaagggcggc
     3001 acgtacctgg ggatggtggc ctcgatcccg ccggaggacc agttcacccg gtggcgatcg
     3061 gagtacgagg ccgacatcat ggtcagcctc tcgtcgctcg caggcgaacg gctgttcttc
     3121 gagggggaca gctcgtcggg cgtctcgggg gacctggagg gcgccacgaa gctggcgacg
     3181 ctcatggagg gctactgggg gatgggcgaa acggtcgcct cccacggcgt gacccacgag
     3241 gtggggatcg gcggtggcgg caagcccggc gacggcaaga agggcaagga gaaggacctg
     3301 ctcgagagcg gcctcggcgg gcggatcgag aagaacctgg agagcctgct ggcgcgggcg
     3361 gagacgctgc tgcgcgagaa ccggcgcgag gtgctggcgg tcgcccatgc cctggagacc
     3421 aacaagaccg ttaccggcga cgacgtggag gcgatcatcg agggccgccc ggggcttctg
     3481 atcgacggct cggtgtacgc gtcggacgac ttcctggcgc tggccgagga ctaccacacg
     3541 gccgtcgtct cggcgcacaa gacccacgac aaggtgtcgg tgccgctccc ggtgctgaac
     3601 ggacagcgcc gggcgctcga gcccggcgag ctcgtcgcgg ccggcgggtc gtcgccggac
     3661 gggccgtcgg ggaacggccc cggcgccgcg gacccgccgg cgccgcccgc gccgggtctc
     3721 gcggcacctc caccgcccgg gctcccgagc gtcgtccacg acaccacggt cctggtcgag
     3781 ggagcgtgcc cgaactgcgg cagggtcaac gccgaatggg cccgcttctg cacacgctgc
     3841 ggcacggaac ttcgcccccc gcagccctga ccccgaccta tattgcgggc gtgagctgcc
     3901 cgaactgcgg cgcggagaac gcgccgggcg cgcggttctg ctcccgttgc ggcgcctcgc
     3961 tcgcccgccg ctgcgcgaac tgcggggccg acctggccga aggggcccgc ttctgctcgg
     4021 cctgcgggac gccggtcgag cccgaggcgg ccgagctcgc cgaggaacgc aagctcgtca
     4081 cgatcctctt cgccgacgtc accggctcca cccagctcgg cgagcggctc gaccccgagc
     4141 ggatgaagga ggtcatgagc cggttcttcg cagcgatgcg ggccgagatc gaggcggagg
     4201 gcgggaccgt cgagaagttc atcggcgacg cgatcatggc cgccttcggc gtgccggcgg
     4261 cgcacgagga cgacccctcg cgggcgctgc gcgcggtgat gcgcatgcgg accagcctcg
     4321 acgcgctgaa ccgcgacctg gagcgcgagc acggcgtgag cctcgccgtc cgcaccggcg
     4381 tcaacacggg cgaggtcatc gcggtgaccg acccgcggcc cggcgaggcg ctcgccaccg
     4441 gggacgcggt gaacgtcgcc gcccggctcg agcaggcggc cgagccgggg caggtgctcg
     4501 tcgccgagcg caccgcccgc gccgcgcgcg gcttccggtt ccgcgacctc ggcccggtct
     4561 cggtgaaggg caaggaggaa cccgtgcgag cgctggagct gctcggcacc gccgagggcg
     4621 cggagggcgc ccagcgcggg atcccggggc tgcgcgcccc gatggtcggg cgcgacgagg
     4681 agctctccct gctgcggacc gtgtacaggc ggctggccgc cgaggggcag ccgaacctgg
     4741 ccaccatcta cggcgacccc ggggtcggga agagccgcct ggcccgggag ttcgcggcat
     4801 gggtcgaggt ccaggagccc cgaccgacgg tcctcgccgg ccgctgcctc ccctacggcg
     4861 agggcgtcac gtactggccg ctggcggaga tcctgaagtc ccacgccggg gtgctcgaca
     4921 gcgatccgcc cgaccgcgcc ctcgcccgca tccgcgcggc gacggaggcg ctgctggcca
     4981 ccgaggccgc cgcggacccc gcccgcaccg ccgcggcgct cgccttcgcg atggggctcg
     5041 aggacccggc gttccgcctg gccgacctcg cgccgcgcca ggtccggctg gagacgcaca
     5101 atgcctggcg ggcgttcttc tcggagatcg cccggccggc gccggcggtg gtggtcgtcg
     5161 aggacatcca ctgggccgac ccggcgctgc tcgacctgct cgaggacctc gccgaccggg
     5221 ccctcggccc ggtgctgttc ctgtgcccgg cccgccccga gctgaccggg cggcgcgccg
     5281 ggtggggcgg cggccgccgc aacttcacct cgatcttcct cgagccgctg tcgcacgagg
     5341 atgccgaccg gctggtgggc ttcctgctgg cgatcgagga tctgcccgag tcggtgcacg
     5401 agcggatcct ggctcgcgcc gagggcaacc cgttcttcct ggaggagatc ctccgccgcc
     5461 tgatcgacga ggggcggatc gtccgcgagg gcgatcgctg gcgggcctcc gcggcgatcg
     5521 gcgaggtcga gatccccgat accgtgcagg ccgtcctcgc cgcccggatc gacctgctcg
     5581 cgtcggagga gaagcgcgcg ctgcagtcgg ccgcggtcgt cggacgcgtg ttctggtccg
     5641 gccccgtcgg cgagctcctg aacggcgagg gagagcgcct ggaggagatc ctgcagcgcc
     5701 tcgaggaccg cgagctggtg tcggctcggc tcggctcctc gctggccggc gagcgcgagt
     5761 acatcttcaa gcatgtcctg acccgcgacg tggcctacga gagcctgccg cgccgggagc
     5821 gggcggtggc ccacgcagag gtcgcgtcgt ggatcgagcg tacggccggc gagcgacgat
     5881 cggagttcgc ggagctgctg gcgtaccact acagcgaggc gcaccgcagc gccggcgcga
     5941 ccggcgatcg ggaggggctg cggcggaagg cgttcgagta cctcgtcgcc gcagcgcgtg
     6001 gcgcccgcgc caagttctcc gtggccacgg cggtgcggct cggggaggag gcggcctcgc
     6061 tggccgacgg ccccctggaa cggtcgcggg ccctggaggt cctgggcctg acctacatcg
     6121 acgactaccg cggcttccct gcctacgaca cgctgcgcgc ggcggtcaag gagcgcatcg
     6181 ccggcgaccc gaccgaccac ctggggatcg cgatgctgtg cgcccgtgcg atcgaaacgc
     6241 cgacgcgctg gcccggcgcg atgcaggggt tgccgccggc ggccgaggcg gcctcctacc
     6301 tggccaccgg cctcgagcac gccggcaccg ccgactccga ggcgcgcctc aggctgctca
     6361 cggcccgggc gttctggccg ttcgcgtacc cggacgacac cccgcccgag gagtggcagg
     6421 aggcggagcg ggcgggtgag gaggccga
//
LOCUS       DASPWO010000401         5488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_228069, whole genome shotgun sequence.
ACCESSION   DASPWO010000401 DASPWO010000000
VERSION     DASPWO010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228069"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3435
                     /locus_tag="VEO00_09200"
     CDS             <1..3435
                     /locus_tag="VEO00_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYU58209.1"
                     /translation="RGFKSFALKTSLEFAPGVSVIVGPNGSGKSNLVDAITWVLGEQG
                     PRALRGASMADVIFAGSPKRPALGMAEVHLVIDNSAGLIPVPLAELEISRAVYRSGES
                     QYFIGRKPVRLMDVQELLAESGIGRELHTVVGQNQLEDVLTARPEERRRFIEEAAGIA
                     KHRRRRERAERRLASVEQDVLRLQDVLAELNRQLRPLRRQAESAKRHDALQQEAAAIA
                     ARLAAVSLRDLYAVRDARRGGWDAGLARRRDLEERLAGLDERLAELARAREPTEARLA
                     AAAAKVEAATEARWEAEAAVRAAVRAEADARAGLAEHADRAAHLALLAGEARRAGEAL
                     EAVRAALAEREPHLAVAEAEFAEAARAREAAESEARRAHEEATGRRAEAEALGRSLEA
                     YERERGLLAASLGELERRIARAEADRVALEGEVERLDAAETPLSRDQARLERERTALH
                     EEVSGLREREARLSARLAGLAERAAVRSETPARRFVRERPGRAPGLLSDLIRAEPGYE
                     RALAAALGALSDAVAAESLELAVEDVRGAAGVTFAVGHAAGGWGLGQVARPVARSAGP
                     RPASEPLEGDVPAGPETLLEHLRVDPKAAGIAGSLLRDVYLADDPADALRGARQRPGA
                     RFVTTAGELVGPDIVRAVGKAGEAERANAAAREAAERELREVTAGLRRREQALAAADA
                     DLAALAGRLDEADARITAAADRMGALGADLASLTRERQVVSERLAALDEAAGAWRDAL
                     AAIPPPSPEPAAPPERPEPPMALRVEVEAMRRDAARLEVEAARARAELDAARSEDPEA
                     LAASSAAAERARAAAEEALAEAEAGRSAAALARADVERAEGEIRRAEDEANHAWREVS
                     TELARLRDEYEEEDQARRDLERRIEEAERVLHEGHRRAPAEAVSELTEDDAAELLRRR
                     ADLVARRLALLGRVNLLAGDELEALQERHDFLARELEDVRGTRRELLTLIEDVDRRIL
                     ATFDAAFRDVATAFEDLFAGLFPGGEGRIALTEHEDPMQRGVDIEARPGRKRVKRLSL
                     LSGGERALTAICFLFAIFRARPSPFYLLDEVEAALDDINLHRFLDLVRGFAATSQVLL
                     VTHQKRTMEIADVLYGVSLGADGVSTVIAQRLERDRFEQNGPATPDEPVVTATEAG"
     gene            3445..4449
                     /gene="ftsY"
                     /locus_tag="VEO00_09205"
     CDS             3445..4449
                     /gene="ftsY"
                     /locus_tag="VEO00_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HYU58210.1"
                     /translation="MPTAVGLEIVVVLALLALLVAAFVAARWSRRRETRPTARRARRR
                     PARGGPAGREALGGRIRDLFARRGGEPDVWRELEDLLVRADVGPVAAASLVGRVRERF
                     APGDDPARLVAAEVEALFAGDPRLRVGEGHPSIVLVVGVNGSGKTTTIGKIAHRLVRE
                     GRTVCLAASDTFRAAAVEQLATWAERAGADLVAQQRGADPGAVAFDALQAARARRADV
                     LIVDTAGRLHTRTPLMDELAKVRRVLEKAGGTIDETLLVLDATTGQNGIVQARTFTEA
                     AGVSGIVLTKLDGTARGGVVVAVREALGIPVKLVGSGEGIEDLEPFDPERFARALADV
                     "
     gene            4465..4830
                     /locus_tag="VEO00_09210"
     CDS             4465..4830
                     /locus_tag="VEO00_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYU58211.1"
                     /translation="MATILVVDDDDGIRLVMKAILEQDGFTIVEAGDGESALEIALAD
                     PPDLIFLDLMMPGIDGIEVLKRLRADERTAKVDIVVVSARGAEGRPDAMAAGADDYFE
                     KPFSPLALLQTVERRLGSA"
     gene            4970..>5488
                     /locus_tag="VEO00_09215"
     CDS             4970..>5488
                     /locus_tag="VEO00_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HYU58212.1"
                     /translation="MFDTLTGRLESVFARLRKRGKLHPQQVDLALADIRTALLDADVA
                     VAVADDLLARVRARAVSEEVMRSLTPAQQVVKVVRDELTTTLGGEHRPFVLPPASPAV
                     VMLAGLQGSGKTTQAAKLARHLKEKGRRPLLVAADLARPAAIEQLFALGAEAGVPVVS
                     EGRDAPRVARAAL"
BASE COUNT          677 a         1968 c         2199 g          644 t
ORIGIN      
        1 cgtggcttca agtcgttcgc cctgaagacc tccctggagt tcgccccggg cgtctcggtg
       61 atcgtcggcc cgaacggctc cggcaagtcg aacctggtgg acgcgatcac ctgggtgctc
      121 ggcgagcagg gcccgcgcgc gctgcgcggc gcgagcatgg ccgacgtcat cttcgccggc
      181 agcccgaagc ggcccgcgct ggggatggcc gaggtccacc tggtgatcga caactcggcc
      241 gggctgatcc ccgttccgct cgccgagctg gagatctcac gcgccgtcta ccgctctggc
      301 gagagccagt acttcatcgg tcgcaagccc gtccggctga tggacgtcca ggagctcctg
      361 gccgagagcg ggatcggccg ggaactgcat accgtggtcg ggcagaacca gctcgaggat
      421 gtgctgaccg cgcggccgga ggagcgccgc cgcttcatcg aggaggccgc cggcatcgcc
      481 aagcaccgcc gccgccgcga gcgcgccgag cgtcgccttg cgtcggtaga gcaggacgtg
      541 ctgcggctgc aggacgtgct ggccgagctg aaccggcagc tgaggccgct ccggcggcag
      601 gccgagtcgg ccaagcgcca cgatgccctg cagcaggaag ccgcggcgat cgccgcgcgc
      661 ctggccgcgg tctcgctccg cgacctctac gcggtgcgcg acgcgcgccg cgggggctgg
      721 gacgccggcc tggcccggcg acgggacctg gaggaacggt tggccgggct cgacgagcgc
      781 ctggccgagc tcgcccgggc gcgcgagcca acggaggcgc ggctcgccgc cgccgcggcg
      841 aaggtggaag ccgccaccga ggcccgctgg gaggccgaag cggcggtccg cgccgcggtg
      901 cgggccgagg ccgatgcgcg ggccggcctg gcggagcacg cggaccgcgc ggcccatttg
      961 gcgctgctgg cgggggaggc gcgccgcgcg ggcgaggcgc tcgaggccgt tcgggccgcg
     1021 ctggcggagc gggagccgca tctcgcggtg gccgaggccg agttcgccga ggcggcccgg
     1081 gcgcgggagg cggccgagtc cgaggcccgc cgtgcccacg aggaggcgac tggacgccgg
     1141 gcggaggccg aggcgctggg gcggagcctg gaggcctacg agcgcgagcg gggactcctt
     1201 gccgcctcgc tgggggagct ggaacggcgc atcgcccggg ccgaggcgga tcgcgtggca
     1261 ctggagggcg aggtcgagcg cctcgacgcg gccgagacgc cgctgtcgcg cgaccaggcg
     1321 cggctggagc gcgagcggac ggcgctgcac gaggaggtgt cggggctgcg ggagcgggag
     1381 gcgcgcctct ccgcgcgcct cgcgggcctc gcggagcgcg ccgccgtccg ttcggagacg
     1441 cccgccaggc ggttcgtccg ggaacggccc gggcgtgcgc ccgggctcct gtccgatctg
     1501 atccgcgccg agccgggcta cgagcgcgcc ctcgcggctg cgctcggcgc cctgtccgac
     1561 gccgtggccg ccgagagcct ggagctcgcc gtggaggatg tgcggggagc ggcgggcgtg
     1621 acgttcgcgg tgggccacgc cgccggcggg tgggggctcg ggcaggtagc tcgccctgtg
     1681 gcgagatcag ccggaccacg tccggcgagc gaacccctcg agggggacgt gcccgccggg
     1741 cccgagacgc tgctggagca cctgcgcgtc gatccgaagg ccgccgggat cgccggttcg
     1801 ctgctgcgcg acgtgtacct ggcggacgat ccggccgacg ccctccgggg cgcccggcag
     1861 cgtccggggg ccaggttcgt cacgaccgcc ggcgagctcg tcggcccgga catcgtccgc
     1921 gccgtcggga aggccggtga ggccgagcgg gccaacgcgg cggcgcgcga ggccgccgaa
     1981 cgcgagctgc gcgaggtcac ggccgggctg cgccgccgcg agcaggcact ggccgcggcc
     2041 gacgccgatc tggcggcgct ggcgggtcgc ctggacgagg ccgacgcccg gatcaccgcg
     2101 gccgccgacc ggatgggggc gctcggcgcg gacctcgcct cactgacccg cgagcgccag
     2161 gtcgtctcgg agcggctggc ggcccttgac gaggcggccg gcgcctggcg cgacgcgctc
     2221 gccgcgatcc cgccgccgtc ccccgagccg gcggcgccgc cggagcggcc cgagcccccg
     2281 atggcgctgc gcgtggaggt cgaggccatg cgccgggatg ccgcccggct ggaagtggag
     2341 gccgcgcggg cccgggccga gctcgacgcg gcccggtcag aggacccaga ggcgctggcg
     2401 gcctcctccg ccgccgccga acgggcccgg gcggccgcgg aggaggccct ggccgaagcg
     2461 gaggcggggc gctcggcggc cgccctcgcg cgggccgacg tcgagagggc cgagggggag
     2521 atccggcggg ccgaggacga ggcgaaccac gcctggcggg aggtgtccac cgagctggcc
     2581 cggctgcggg acgagtacga ggaggaggac caggcccgcc gcgacctgga gcggcggatc
     2641 gaggaggccg agcgggtcct gcacgaaggc caccgccggg ccccggcgga ggccgtctcg
     2701 gagctgaccg aggacgacgc cgccgagctg ctccggcgca gggccgacct ggtggcccga
     2761 cggctggccc tgctcgggcg ggtgaacctg ctggccggcg acgagctgga ggcgctgcag
     2821 gagcgccacg acttcctggc ccgggagctg gaggacgtgc gcggaacccg ccgggagctg
     2881 ctgacgctga tcgaggacgt ggaccggcgc atcctggcga cgttcgacgc cgcgttccgc
     2941 gacgtggcga cggcgttcga ggacctcttc gccgggctgt tcccgggcgg cgagggccgg
     3001 atcgcgctca ccgagcacga ggacccgatg cagcgcggcg tcgacatcga ggcgcggccc
     3061 ggccgcaagc gggtcaagcg cctgtcgctg ctctcgggag gcgagcgggc gctcaccgcg
     3121 atctgcttcc tgttcgcgat cttccgcgcc cggcccagcc ccttctacct gctggacgag
     3181 gtcgaggcgg ccctcgacga catcaacctg caccgcttcc tggacctggt ccgcgggttc
     3241 gccgccacca gccaggtcct gctcgtcacg caccagaagc ggacgatgga gatcgccgac
     3301 gtgctgtacg gggtctcgct tggggccgac ggggtgtcga ccgtgatcgc ccagcggctg
     3361 gagcgggatc gtttcgagca gaacgggccg gccacgcccg acgagcccgt cgtcaccgcc
     3421 accgaggccg ggtaacctcc cggcgtgccc accgccgtcg ggttggagat cgtcgtcgtc
     3481 ctcgcgctgc tggcgctcct tgtcgccgcg ttcgtggccg cgcgctggtc gcgccgccga
     3541 gagaccaggc ccacggcgcg ccgcgctcgg cggcggccgg cccgcggcgg cccggccggg
     3601 cgcgaggcgc tcggcgggcg gatccgcgac cttttcgccc gccgcggggg cgagcccgac
     3661 gtctggcgag agctggagga cctgctggtg cgcgccgacg tcggcccggt cgccgcggcc
     3721 tcactcgtcg ggcgagtccg cgagcggttc gccccgggcg acgacccggc ccggctggtg
     3781 gcggcggagg tcgaggccct cttcgccggt gacccgcggc ttcgggtggg ggagggacac
     3841 ccctcgatcg tgctggtcgt cggggtgaac ggcagcggga agaccaccac gatcgggaag
     3901 atcgcgcacc gcctggttcg cgaggggcga acggtgtgcc tggccgcgag cgacacgttc
     3961 cgggcggcgg ccgtcgagca gctcgccacg tgggccgagc gcgccggcgc cgacctggtg
     4021 gcgcagcagc gcggcgccga ccccggggcg gtggccttcg acgcgctgca ggcggcgcgg
     4081 gcgcgccggg ccgacgtgct gatcgtggac accgccgggc gcctgcacac ccgcaccccg
     4141 ctgatggatg agctggccaa ggttcgccgg gtgctggaga aggccggcgg gaccatcgac
     4201 gagacgctgc tcgtcctgga cgccacgacc ggccagaacg gcatcgtcca ggcccgcacg
     4261 ttcaccgagg cggccggcgt ttccggtatc gtcctgacca agctcgacgg taccgcccgc
     4321 ggtggcgtcg tggtcgccgt ccgggaagcg ctcggcatcc cggtcaagct cgtcggctcc
     4381 ggcgaaggga tcgaggatct cgagcccttc gaccccgagc ggttcgcgcg ggccctggcc
     4441 gacgtgtagg acgaaggtgc gctcttggcg acgatcctcg tggtggacga cgacgacggg
     4501 atccggttgg tgatgaaggc catcctggag caggacgggt tcacgatcgt cgaggcaggc
     4561 gacggcgagt ccgcgctgga gatcgccctg gccgacccgc cggacctgat cttcctcgac
     4621 ctgatgatgc cggggatcga cggcatcgag gtgctgaagc gcctgcgcgc cgacgagcgt
     4681 acggcgaagg tcgacatcgt cgtggtctcg gcgcgcggcg cggagggccg cccggacgcg
     4741 atggcggccg gcgcggacga ctacttcgag aagccgttca gcccgctcgc gctcctgcag
     4801 acggtagagc ggcggctggg ctcggcgtaa acggcactgc gttcggcggg gggttggtca
     4861 gcgggatcag caccggaccg aaggccacgg cgaaccggcg cacccgcgcg ttccccggcg
     4921 ctcgagcttc cggagcgatc ccggccgacg tcccgctagc atgtgactca tgttcgacac
     4981 gctcaccggg cgccttgagt cggtcttcgc gcgcctgcgc aagcgaggca agctgcaccc
     5041 gcagcaggtc gacctggccc tcgccgacat ccgaaccgcc ctgctggacg ccgacgtcgc
     5101 ggtcgccgtc gccgacgacc tcctggcccg cgtgcgggcc cgtgcggtct ccgaggaggt
     5161 catgcgcagc ctgacgccgg cgcagcaggt ggtgaaggtg gtccgcgacg agctcaccac
     5221 caccctcggc ggcgaacacc gcccgttcgt gctccccccg gcgagcccgg cggtggtgat
     5281 gctcgccggc ctgcagggtt cggggaagac gacccaggcc gccaagctcg cccgccacct
     5341 gaaggagaag ggccgccgcc cgctgcttgt tgcggccgac ctggcccgcc ccgcggcgat
     5401 cgagcagctg ttcgcgctcg gcgccgaggc cggcgtgccg gtggtgagcg agggaaggga
     5461 cgcgccgcgc gtggcgcggg cggccctg
//
LOCUS       DASPWO010000402         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_569971, whole genome shotgun sequence.
ACCESSION   DASPWO010000402 DASPWO010000000
VERSION     DASPWO010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569971"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2025)
                     /locus_tag="VEO00_09220"
     CDS             complement(<1..>2025)
                     /locus_tag="VEO00_09220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU58213.1"
                     /translation="ATYPAHREAEVVLRDGSTVHVRPVRPDDELAVRAFLAGLSDEAR
                     YFRFFSGSVNLDEQARSATRVDYRDRYGLVATFGADPRIVAHAAFVRTNVDRAEVAFA
                     IDGRFQGLGLGTILLGHLAEAAAENGVAVFEARVLPTNPRMIEVFRESGFPVEIRAEP
                     GEILVEFPASLTEDALERFERREQIAAAAAMRNFLSPRAVAVIGASRSRGTIGGEVFH
                     NLIATGFNGPVYPVNPKAEVVQSVLAFPTVGAIPGPVDLAVIVVPANAVVEAARLCGE
                     KGVRALVVISAGFAETGDEGRERQRELLAVCRDHGMRLIGPNCMGILNTAPDVLLNAT
                     FAPNFPPVGKVGFLSQSGGLGLAVIDYANTLGLGVSSFVSVGNKTDISGNDLIQYWEG
                     DDDTDLILLYLESFGNPRKFARISRRVGRSKPIIAVKSGRSAAGARATSSHTGALIAA
                     SDVTVDALFHQAGVIRTDTLAELFDVASLLANQPAPHGNRVAILTNGGGPGILCADAC
                     EAEGLTVPALSETVRACLSEFLPAEASLANPIDMIASASAEDYARAIETLGNDEDVDA
                     LIVMFVPPLVTRAEDVARAIREAAGSLPPTKPILTVFMSARGVPEELRGEGVRIPSFA
                     FPRGGGSNAGQDRALWHLEGQTRGNRSYVPRRSTGRGRRDRGPSARPRPGV"
BASE COUNT          336 a          708 c          689 g          292 t
ORIGIN      
        1 caccccgggc ctcggccgcg cgcttgggcc acgatcccgg cggcctcgtc ccgtcgagcg
       61 tcggggaacg taggaacgat tccctcgggt ctggccttcc aggtgccata acgcgcggtc
      121 ctggccagcg ttcgagccgc ctcctctggg gaaggcgaac gaggggatcc ggacgccctc
      181 cccccgaagc tcttccggca ccccccttgc cgacatgaac accgtgagga tcggcttggt
      241 cggtgggagg gatccggccg cctcgcggat ggcccgagcc acgtcctcag ccctcgtcac
      301 caacggcggc acgaacatca cgatgagcgc gtcgacgtcc tcgtcgttcc ccagggtctc
      361 gatcgccctc gcgtagtcct cggccgaggc tgaggcgatc atgtcgatcg ggttggccag
      421 cgaggcctcg gcgggcaaga actcggagag gcaggcccga accgtctccg aaagggccgg
      481 gacggtcagg ccctccgcct cgcaggcgtc cgcgcacagg atgcccggcc cgcccccgtt
      541 cgtcaggatg gccacgcggt tgccgtgagg cgccggctgg ttggccagca gcgaggccac
      601 gtcgaagagc tcggccagcg tgtcggtcct gatgacgccc gcctggtgga acagcgcgtc
      661 caccgtcacg tccgatgcgg cgatgagggc accggtgtgc gatgaggtcg cgcgggcccc
      721 cgcggcggac cgaccgctct tgacggcgat gatcggcttc gaccggccga cccggcggct
      781 gatccgcgcg aacttccggg ggttcccgaa cgactccagg tacagcagga tgagatcggt
      841 gtcgtcgtct ccttcccagt actggatgag gtcgttgccg gagatatccg tcttgttgcc
      901 caccgacacg aacgaggaga cccccagccc cagggtattc gcgtagtcga tgacggcgag
      961 accgagaccg ccgctctgcg acaggaaccc gaccttcccg accggcggaa agttcggcgc
     1021 gaacgtcgcg ttcaggagga cgtccggagc cgtgttcagg atccccatgc aattcggccc
     1081 gatcagccgc atcccgtggt cccggcagac cgccaggagc tcgcgctggc gttcccgccc
     1141 ttcgtcaccg gtttcggcaa agcccgccga gatgacgacc agggctcgga cgcccttctc
     1201 tccgcacagc ctggccgcct cgaccaccgc gttcgccggg accacgatga cggcgaggtc
     1261 gaccggcccc gggatggccc cgaccgtcgg aaacgccagg accgactgaa cgacctcggc
     1321 cttcggattc acgggataga ccggcccgtt gaacccggtg gctatgaggt tgtggaagac
     1381 ctcaccgccg atggtgcccc gagacctcga ggcaccgatc acggccaccg cccgaggcga
     1441 caggaagttg cgcatggcgg ccgcggcggc gatctgctcg cggcgctcga agcgctccag
     1501 ggcgtcttcg gtcagcgaag ccgggaactc cacgaggatc tccccgggct cagcacggat
     1561 ctcgaccggg aatccgctct cccggaagac ctcgatcatc cggggattcg tcggcaggac
     1621 ccgcgcctcg aagacggcga caccgttctc ggcggctgcc tcggccaggt ggccgagcag
     1681 aatcgtcccc aaccctagac cttggaagcg accgtcgatg gcgaaggcga cctcggcgcg
     1741 gtcgacgttg gtgcgaacga acgcggcgtg agcgacgatc cggggatcgg cgccgaacgt
     1801 ggcgaccagg ccatagcggt cccgatagtc gacgcgagtg gccgaccgag cttgctcgtc
     1861 caggttcacg gagccggaga agaacctgaa gtagcgagcc tcgtcggaca acccggctag
     1921 gaaggcacga accgccagct cgtcatccgg ccgaaccggg cggacgtgga cggtggagcc
     1981 gtcgcgtagg acgacttccg cctcgcgatg ggcaggatac gtggc
//
LOCUS       DASPWO010000403         4464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1242955, whole genome shotgun sequence.
ACCESSION   DASPWO010000403 DASPWO010000000
VERSION     DASPWO010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242955"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VEO00_09225"
     CDS             <1..462
                     /locus_tag="VEO00_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017579448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYU58214.1"
                     /translation="ALEEAIFLTRFATKVTVVHRRDELRASKIMQDRAFGNEKITFVW
                     DSVIEEVLGNGQVAGARVRNLKTGATSDIAAGGLFVAIGHIPNTALFEGQLELSNGYV
                     VVQEPTTRTSVEGAFAAGDVVDHVYRQAVTAAGMGCKAAIDAERFLESLGH"
     gene            468..1166
                     /locus_tag="VEO00_09230"
     CDS             468..1166
                     /locus_tag="VEO00_09230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYU58215.1"
                     /translation="MRRISAAAPAIVAALAFVPATSPAAAQIPLIPPPAYYRPALSGG
                     MAFPVARSNFFSWIEFGDDWHAPRFRLVDGVWRLVGRHEGIDIGAEKGTPVLSITSGT
                     VERVGWTFYSGTRVGVRGADGRYYFYAHLSAVEAGIAPGAPVGAGEVLGRVGNTGYGD
                     PGTEDQFPPHLHVGIEDGDRWVTPYPLLVRLYAAAVEAGRRDDARLRRIAGSGDRARW
                     DAVRARLYMGISLP"
     gene            1249..1578
                     /gene="trxA"
                     /locus_tag="VEO00_09235"
     CDS             1249..1578
                     /gene="trxA"
                     /locus_tag="VEO00_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HYU58216.1"
                     /translation="MSEIVREVGDGDFQEIVLSSETPVLVDFWADWCVPCHMVSPVVE
                     EIARERSEDIKVAKLNVDDNPATTQKFHVMSIPTLIVFKGGEEMARVIGARGKEAILA
                     EIEPILA"
     gene            1632..2720
                     /locus_tag="VEO00_09240"
     CDS             1632..2720
                     /locus_tag="VEO00_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HYU58217.1"
                     /translation="MRALRPGGEGDAVRDVQSRLAALGYRIDPEEAGRFGPATERVVR
                     EFQQRRGLVVDGIVGVGTWSALVEAGYALGDRTLYLHQPFLRGDDVRALQSRLNAMGF
                     DPGREDGIFGERTDRALREFQRNVGLPADGIVGLTTVEAVGRLRPVGEGPGKAAVRES
                     EALRGARAGLDGALIGVDPGHGPDEPGGVGPTGLTEADATALLGEALAGELRRRGGRP
                     VVLRRTGDDPSIRERAVRANEDALEVVVSIHCNTHPSPDAEGASCYYFGTEGSISRTG
                     ERLAEYIQEELVGRTGVVDGRVHAKAFPMLVETRMPAVQVEPCFITNPREEALLREEA
                     FRRDVAIAIAEGVRRYFSAGEDAEARTG"
     gene            complement(2728..3318)
                     /locus_tag="VEO00_09245"
     CDS             complement(2728..3318)
                     /locus_tag="VEO00_09245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU58218.1"
                     /translation="MGRRILDVTLDDVVRIPPDCLQSCYWELAQDVDGPDARFHKEEW
                     FSMTLLEWGRCGKLAVDGDRSEGFAQYAPPSLFPRLGSFPSAVVSPDAVYLSYCYVIA
                     ERRREGLGTHLVQAVARDLVDRRYRALEALGDRDWVGGFILPAPFLGANGFGVVRDDP
                     RFPLMRLDLRATLDPRRERAAAAIPLAAPAPAPGLA"
     gene            3522..>4464
                     /locus_tag="VEO00_09250"
     CDS             3522..>4464
                     /locus_tag="VEO00_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HYU58219.1"
                     /translation="MATSDLEVFADLFAARVKGMSASEVRALFAVASRPEVVSLAGGM
                     PYVQALSTEAVLAAIRTVIEERGAVALQYGSGQGHPGLREQLAMIMAAEGIEADPDHL
                     VVTDGAQQGLDLVAKLFIDPGDTIVVEAPAYVGALSAFSAYEPAYFQVPLDDDGMQVE
                     VLATALAGGLRPKFVYTVPNFNNPAGVTLSYERRRRLADLCRHHGVPILEDNPYGMLR
                     FDGDPVPALRTLDPGNVIYLGTVSKVFAPGVRIGWILARPDILEQLILQKEAANLCSS
                     QLTQLVTEEYFAHQDWRGGLTALVEAYRGRRDAMLDAL"
BASE COUNT          638 a         1590 c         1634 g          602 t
ORIGIN      
        1 gctctcgagg aagccatctt cttgacgcgg ttcgccacga aggtcacggt cgtccaccgc
       61 cgcgacgagc tccgcgcctc gaagatcatg caggaccgcg ccttcggcaa cgagaagatc
      121 accttcgtct gggattcggt gatcgaggag gtgctcggga acgggcaggt cgccggcgcg
      181 cgcgtgcgca acctgaagac cggcgcgacc agcgacatcg cggccggcgg cctgttcgtc
      241 gccatcggac acatcccgaa caccgcgctg ttcgaggggc agctcgagct gtcgaacggg
      301 tacgtcgtcg tccaggagcc gaccacccgg acgagcgtcg agggcgcgtt cgccgccggc
      361 gacgtcgtcg accacgtcta ccggcaggcc gtcaccgccg ccgggatggg ctgcaaggcc
      421 gccatcgacg ccgagcgctt cctggaatcc ctcggccact gagagtcgtg cgccggatca
      481 gcgcggcggc cccggcgatc gtggccgcgc tggccttcgt cccggccacc tcccccgccg
      541 ccgcgcagat ccccctcatc ccgccgccgg cctactaccg gccggcgctc tccggcggga
      601 tggcgttccc ggtggcgcgc tcgaacttct tctcatggat cgagttcggc gacgattggc
      661 acgcgccccg gttccggctg gtggacggcg tgtggcgcct ggtcggccgg cacgaaggca
      721 tcgacatcgg ggcggagaag gggacgccgg tgctgtccat caccagcggc accgtggagc
      781 gcgtcgggtg gacgttctac tcggggacgc gcgtgggcgt ccgcggcgcc gacggccgct
      841 actacttcta cgcccacctg tcggcggtgg aggccgggat cgcgccggga gctccggtcg
      901 gcgccggcga ggtgctcggc cgcgtcggca acaccggcta cggcgacccc ggcaccgagg
      961 accagttccc accgcacctg cacgtcggca tcgaggacgg cgaccggtgg gtgacaccct
     1021 acccgctgct ggttcggctg tacgccgccg ccgtggaggc cggccgccgc gacgacgcgc
     1081 gcctccgacg gatcgccggc agcggcgacc gggcgcgctg ggatgccgtt cgcgcccgcc
     1141 tgtacatggg aatatcgctc ccgtaggccg gcgttcaacg gttcggagcg ttgaccacgg
     1201 tgcctcatgc gagacgcccg gcgctcgtga gaaccacagg agacgaacgt gagtgagatc
     1261 gtgagggagg tcggggacgg cgacttccag gagatcgtcc tgtcgtcgga gaccccggtc
     1321 ctggtggatt tctgggccga ctggtgcgta ccgtgccaca tggtgtcgcc ggtggtcgag
     1381 gagatcgccc gcgaacggag tgaggacatc aaggtcgcca agctgaacgt ggacgacaac
     1441 ccggccacga cccagaagtt ccacgtgatg tcgatcccga cgctgatcgt gttcaagggc
     1501 ggcgaggaga tggccagggt gatcggcgcg aggggcaagg aagcgatcct cgccgagatc
     1561 gaaccgatcc tggcctgagc cggaggacgc tccgcgggcg gccgttccgg ccgcctgcga
     1621 gacttgtctc catgagagcg cttcgcccgg gcggcgaggg ggacgcggtg cgcgacgtgc
     1681 agtcgcgcct cgcggcgctc ggctaccgca tcgatcccga ggaggctggt cggttcgggc
     1741 cggccaccga gcgggtcgtc cgggagttcc agcagcgccg cggcttggtc gtcgacggga
     1801 tcgtcggcgt cggcacgtgg tcggcgctcg tcgaggccgg gtacgcgctc ggggaccgca
     1861 ccctctacct gcaccagccc ttcctgcgcg gggacgacgt gcgcgcgctc cagtcgcggc
     1921 tgaacgccat ggggttcgat cccggacggg aggacggcat cttcggcgag cggaccgacc
     1981 gtgccctgcg ggagttccag cgcaatgtcg gcctcccggc ggacggcatc gtcgggctca
     2041 ccacggtgga ggcggtcggc cggctgcgtc ccgtcggcga gggtccgggg aaggcggctg
     2101 tgcgcgagag cgaggcgctg cgcggcgcgc gggccggact cgacggggcg ctgatcggcg
     2161 tcgaccccgg ccacggcccg gacgagccgg gcggcgtcgg cccgaccggc ctgacggagg
     2221 ccgacgccac cgcgctgctc ggggaggccc tggccggaga gctccgccgc cgggggggcc
     2281 gcccggtggt cctgcgccgt acgggcgacg acccctcgat ccgcgaacgg gccgtacggg
     2341 cgaacgagga cgcgctggag gtcgtggtct cgatccactg caacacgcat ccctcccccg
     2401 acgcggaggg cgcctcctgc tactacttcg gcacggaggg ctcgatctcg cggaccggcg
     2461 agcgcctcgc cgagtacatc caggaggagc tcgtcggtcg gaccggcgtc gtcgacgggc
     2521 gcgtccacgc caaggcgttc ccgatgctcg tcgagacccg catgcccgcc gtgcaggtgg
     2581 agccgtgctt catcaccaac ccccgcgagg aggcgctgct gcgcgaggag gcgttccggc
     2641 gggacgtggc catcgccatc gccgagggcg tccgccggta cttctctgcc ggggaggacg
     2701 ccgaagcgcg gaccgggtag gccgggttca ggccagtccg ggcgccggcg cgggtgcggc
     2761 cagcgggatg gcggcggcgg cgcgctcgcg cctcgggtcg agcgtggccc gcaggtcgag
     2821 gcgcatcagc gggaagcgcg gatcatcgcg caccacgccg aagccgttcg cgccgaggaa
     2881 cggcgccggc aggatgaagc cgccgaccca gtcccggtcg ccgagcgcct cgagcgcccg
     2941 gtaccgccgg tcgaccaggt cgcgcgccac cgcctggacc aggtgcgtgc ccaggccctc
     3001 gcgccgacgc tcggcgatga cgtagcagta cgagaggtac accgcgtcgg gcgagacgac
     3061 cgccgagggg aagctgccca gccgcgggaa cagcgagggc ggcgcgtact gcgcgaaccc
     3121 ctccgatcga tcgccgtcga ccgcgagctt cccgcaccgt ccccactcga ggagcgtcat
     3181 cgagaaccac tcctccttgt ggaagcgcgc gtcggggccg tccacgtcct gcgccagctc
     3241 ccagtagcag gactgcaggc agtccggggg gatgcggacg acgtcgtcga gcgtgacgtc
     3301 gaggatgcgc cggcccaccg ccgcctccga gcctcagcgg ctcacgggag gtccgaccgc
     3361 cggccgaaga ccagcccgag caccaggccc aggaccgccc cgagcagcag cgcccggagc
     3421 gcgcggccca cttcctcacg gttcggcacg ttctgtagcg tacgccacgg cgagcgaccc
     3481 tccgaaacgg cggcgaagcc ctgctgtacg attccccgcc catggccacg tccgacctcg
     3541 aggtcttcgc cgacctgttc gccgcgcgcg tgaagggcat gtcggcctcg gaggtgcgcg
     3601 cgctcttcgc ggtggcctca cggccggagg tggtgtcgct cgccgggggg atgccctacg
     3661 tccaggcgct ctcgaccgag gcggtgctcg cggcgatccg gaccgtcatc gaggagcgcg
     3721 gcgccgtcgc cctgcagtac ggcagcgggc agggccaccc gggcctccgc gagcagctgg
     3781 cgatgatcat ggccgccgag ggtatcgagg ccgatcctga ccacctggtg gtcaccgacg
     3841 gcgcgcaaca gggactcgac ctggtggcga agctgttcat cgaccccggg gacacgatcg
     3901 tggtcgaggc gccggcctac gtcggtgcgc tctcggcctt cagcgcgtac gagccggcgt
     3961 acttccaggt ccccctggac gacgacggca tgcaggtcga ggtcctcgcc acggcgctgg
     4021 cggggggcct gcgcccgaag ttcgtctaca ccgtgccgaa cttcaacaac ccggcaggtg
     4081 tgacgctatc atacgagcgc cggcggcggt tggccgacct ctgccgccac cacggcgtgc
     4141 ccatcctgga ggacaacccc tacggcatgc tccggttcga cggtgacccg gtcccggcac
     4201 tacggaccct cgaccccggg aacgtgatct acctcggcac ggtctcgaag gtgttcgcgc
     4261 cgggtgtacg gatcggatgg atcctggccc gccccgacat cctcgagcag ctcatcctgc
     4321 aaaaggaggc cgccaacctc tgctccagcc agctgacgca gctggtcacc gaggagtact
     4381 tcgcgcacca ggactggcgc ggcggcctga cggcgctggt cgaggcctac cgcggccggc
     4441 gcgacgcgat gctcgacgcg ctcg
//
LOCUS       DASPWO010000404         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_907804, whole genome shotgun sequence.
ACCESSION   DASPWO010000404 DASPWO010000000
VERSION     DASPWO010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907804"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VEO00_09255"
     CDS             <1..204
                     /locus_tag="VEO00_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58220.1"
                     /translation="RLGPRPVSIGQRTPLWAHSPTALAMKWTAIAMISSPTTRLMARS
                     RIGRSRDGAHRNSPEALIEIPHP"
     gene            complement(217..1008)
                     /gene="istB"
                     /locus_tag="VEO00_09260"
     CDS             complement(217..1008)
                     /gene="istB"
                     /locus_tag="VEO00_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016695443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HYU58221.1"
                     /translation="MSHDTLYQRLRSHLAYLKLPAAAEALAGKLDAAEKANPSYTAFA
                     EDLLGAEVEATERRRLAGRLRFSHFPVIKRLEDFDFDAQPGLDRRLVEELATLRFVEE
                     RANVLMVGPPGVGKTMLALALGYRAIEAGYRVYYTTAADLAARCHKAAIEGRWATTMR
                     FFAGPQLLICDELGYLPLQSEAASAIFQVVSRRSEQGSMVVTTNRSIASWGEIFDDQM
                     MAAAILDRFLYKATLLAITGDSYRMRAHRERLQQLREGVAATLER"
     gene            complement(1005..>2183)
                     /locus_tag="VEO00_09265"
     CDS             complement(1005..>2183)
                     /locus_tag="VEO00_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HYU58222.1"
                     /translation="RSYLTAKTTPGVRRRGTDPFARIEPYVRQRLEDDPHLQLAVLIR
                     ELGPLGFTASYQTLTREVRARSLRPHCEACAGAKGRATTDIEHSAGEETQWDWLELPD
                     APWARKLIILTGTLAYSSKTRAWISEAKDTPHLLQGVHEVLTRLGGLTTAFRIDAMEG
                     AVVPGTRRLCAAFADAARFFGVSVIMCPPRRGNRKGVVEKNNDHIAQSWWRTADVTTP
                     EQAQASLDAFCASVGDRRSRGGATVGALAEREQLRPLPAGPYPVVVESARKVSWAALV
                     SYEGNRYSVPATFVNAWVAVSVRLGGPVVDIRSASGEALARHRRRPPGAGAMVRSEEH
                     RAGLERTVLAQFSTARPCRRKEHRPPSDEARAIAARITRGVQQSFDVDLVRYEELMAP
                     "
BASE COUNT          313 a          752 c          809 g          309 t
ORIGIN      
        1 cgattggggc cccgcccggt ctcgatcggt cagcgcacgc cgctgtgggc ccattcgccc
       61 acggcgctcg cgatgaaatg gacggcgatc gccatgatca gcagcccgac cacacggctg
      121 atggcccgca gcaggatcgg gcggagccgt gacggcgctc atcgaaattc cccagaggcg
      181 ctcatcgaaa ttccccaccc ctgagaccgg ccgagtctag cgctccagcg tggcggccac
      241 cccctccctg agctgctgca gtcgctcccg gtgggcccgc atccggtagg agtcgccggt
      301 gatcgcgagg agcgtcgctt tgtagaggaa ccggtcgagg atcgccgcgg ccatcatctg
      361 gtcgtcgaag atctcgcccc aggaggcgat cgagcggttg gtggtcacga ccatcgagcc
      421 ctgctccgag cgccgcgaca cgacctggaa gatcgccgag gccgcctcgg actgcagggg
      481 caggtagccg agctcgtcgc agatcagcag ttggggaccg gcgaagaacc gcatggtggt
      541 ggcccaccgg ccctcgatcg cggccttgtg gcaccgggcg gcgaggtcgg cggcggtggt
      601 gtagtacacg cggtagccgg cctcgatggc ccggtacccg agcgccaggg cgagcatcgt
      661 cttgcccacc ccgggtggcc ccaccatcag cacgttggcc cgctcctcca cgaaccggag
      721 cgtggcaagc tcctcgacca ggcgccggtc cagaccgggc tgggcgtcga agtcgaagtc
      781 ctcgagtcgc ttgatcaccg gaaagtggga gaaccggagc ctgccggcca ggcgcctgcg
      841 ctcggtggcc tcgacctctg cgccgagcag gtcctcggcg aacgcggtgt aggaggggtt
      901 ggccttctcc gcggcgtcga gcttcccggc gagggcctcc gccgccgccg ggagtttcag
      961 gtaggcgagg tgggagcgca gtcgctggta gagcgtgtcg tggctcatgg ggccatcagc
     1021 tcctcgtaac ggacgaggtc gacgtcgaag gactgctgga cgccccgggt gatccgggcg
     1081 gcgatggccc tcgcctcatc cgacggtgga cggtgctcct tgcgccggca tggccgcgcc
     1141 gtggagaact gggccagcac cgtccgctcc aggccggccc ggtgctcctc ggagcgaacc
     1201 atggcgcccg cccccggtgg ccggcggcgg tgccgggcga gcgcctcgcc cgaggcggag
     1261 cggatgtcca caaccggccc cccgagacgc accgagacgg cgacccacgc gttcacgaag
     1321 gtcgccggga cggagtagcg gttcccctcg taggagacga gagccgccca gctcaccttc
     1381 cgggcggact ccaccacgac cgggtacgga ccggcgggaa gggggcggag ctgctcgcgc
     1441 tcggcgaggg caccgaccgt ggcgccgccg cgggacctac ggtcgccgac cgaggcgcag
     1501 aacgcgtcca ggctcgcctg cgcctgctcc ggcgtcgtca cgtcggccgt gcgccaccag
     1561 gactgggcga tgtggtcgtt gttcttctcc accacgccct tgcggttgcc ccggcggggc
     1621 gggcacatga tcaccgagac gccgaagaac cgggcggcgt cggcgaacgc cgcgcacagc
     1681 cgccgggtcc ccgggacgac ggccccctcc atggcgtcga tgcggaacgc cgtggtgagc
     1741 ccgccgagtc gggtgaggac ctcgtggacg ccctgcagca ggtgcggggt gtccttggcc
     1801 tcggagatcc acgccctggt cttcgaggag taggccagcg tcccggtgag gatgatgagc
     1861 ttgcgtgccc acggcgcgtc ggggagctcg agccagtccc attgggtctc ctccccggcc
     1921 gagtgctcga tgtcggtcgt ggcgcggccc ttggcgccgg cgcacgcctc gcagtgaggc
     1981 cggagcgacc gcgcccggac ctcgcgggtg agggtctggt agctcgcggt gaacccgagc
     2041 ggcccgagct cacggatgag gacggcgagc tgcagatgtg ggtcgtcctc cagtcgctgc
     2101 cgaacgtaag gctcgatgcg agcgaacgga tcggtcccgc ggcggcggac tccgggggtg
     2161 gttttggcgg tgaggtagga ccg
//
LOCUS       DASPWO010000405         4183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_570387, whole genome shotgun sequence.
ACCESSION   DASPWO010000405 DASPWO010000000
VERSION     DASPWO010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570387"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VEO00_09270"
     CDS             complement(<1..797)
                     /locus_tag="VEO00_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYU58223.1"
                     /translation="MGMLDGKVAVITGAGRGIGRGEALLFAAEGARVVVNDLGGEWDG
                     TGADARPAAQVVEEIRAAGGEAVAHFEDISEPEGATSLIGAALDAWGRLDAVVNNAGI
                     LRDRMIFNQSVEEWDAVIKVHLRGHFLVTREACAHWRERAKAGEETSGRIVNTSSTSG
                     ILGMVGQSNYGAAKAGIAAFTQIVAMEMGKYGVTANAIAPGARTRLTEMTFGDLSVPE
                     GQFDALAPENVAPLVVYLASDRAAHISGQVFGIQGGLVQLYRGWTPGS"
     gene            843..1670
                     /locus_tag="VEO00_09275"
     CDS             843..1670
                     /locus_tag="VEO00_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HYU58224.1"
                     /translation="MTAGASPAAWHADPYGRHELRYWDGVRWTEHVSDSGRQSLDYPD
                     AERQGVPTVARSTARITRDVARAGVAAVGDGGGSVFTEPVLVVNQKAKLMELTAQYAV
                     FDRNGTQIGGVRQVGQSAGRKVLRALTSLDQFLTHRLEIVDMSGAVLLRLVRPAKVMK
                     SRILVQDPHGNEVGQIVQQNVIGKIRFSLEAGGAAVGSLNAENWRAWNFSIRDAEGAE
                     VARITKTWEGLAKTMFTTADNYVVEIMRRLEDPLRALVVASALSVDTALKQDARGLG"
     gene            1735..2211
                     /locus_tag="VEO00_09280"
     CDS             1735..2211
                     /locus_tag="VEO00_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58225.1"
                     /translation="MTQVRTTVADQHYVLEASEIERRLRDESPEPIAEHYVVVAGRRF
                     PPKQVIGLITGLDRADFNTHQARRILSRLGFTMGRRSKDALPSGPDRPGPYRGREADL
                     LRPFMGRWVAQRGLEILVDADSPQEVLAWLERHRQRADAVFRVPADETEATGVAPA"
     gene            2208..2603
                     /locus_tag="VEO00_09285"
     CDS             2208..2603
                     /locus_tag="VEO00_09285"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HYU58226.1"
                     /translation="MRFPFKELPGRGRDYLRPAVPVTVEGISLAPQLCLLDTGALHNR
                     FGAWVAEAAGIDLGGAEQQRLAVGGFVTAARAAPVQLTLGDVTWEAPTWFCDPWPLAF
                     HLLGQEGFFRWFEVCLRAGAYEIEVTPGD"
     gene            complement(2637..3152)
                     /locus_tag="VEO00_09290"
     CDS             complement(2637..3152)
                     /locus_tag="VEO00_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58227.1"
                     /translation="MPAETVPETRVEHEAFGREERARRAWAVIALFAVATALATALLT
                     FPQDIQTSGLAELQSKAQEARDFFTADYVFIVLYGLLGPVALWRFGRAIGRDGHPPLW
                     LRLGILFLAGGGIVDALENTLIVLSLQATGDGALVAAHALAIPKYAFAFPGFALALVA
                     LVRAIRSLATR"
     gene            complement(3164..3847)
                     /locus_tag="VEO00_09295"
     CDS             complement(3164..3847)
                     /locus_tag="VEO00_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HYU58228.1"
                     /translation="MTVPRLSAAAATAEVALRDPKLGRIIELAGPFRVTKTRRTESAF
                     FASLVESIMYQQLAGAAAAAIHGRFRALFDGELAPEALLAMPDERIRGAGVSGAKLAS
                     IKDLAAKVADGTVPLDGIEKLPDDEIVARLSQVRGIGRWTAEMFLIFDLRRLDVWPVD
                     DYGVRGGWAIAHGLAEVPKPKELEPLGDRFRPWRTIAAWYCWRAVHLSRTGGLPAVRR
                     RRKPALPPG"
     gene            complement(3844..>4183)
                     /locus_tag="VEO00_09300"
     CDS             complement(3844..>4183)
                     /locus_tag="VEO00_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU58229.1"
                     /translation="AGDDVQACRDLVKPFLALYVGGMGARGRNFYTNLVQRYGYEAEA
                     EKVQELYLSGKKLEAAGAVPDALVDEVSLVGPSERIGDRLAAWREAGVTTLIAGALQV
                     DAIKALAAAA"
BASE COUNT          612 a         1534 c         1462 g          575 t
ORIGIN      
        1 gaccccggcg tccacccccg gtacagctgc accaggccgc cctgtatccc gaagacctgg
       61 ccggagatgt gggcggcccg gtcggaggcc aggtagacca cgagcggcgc cacgttctcg
      121 ggcgccaggg cgtcgaactg cccttcgggc acggagaggt cgccgaacgt catctcggtc
      181 agccgggtgc gcgcgcccgg ggcgatggcg ttcgccgtca ccccgtactt gcccatctcc
      241 atcgccacga tctgcgtgaa cgccgcgatg cccgccttcg ccgcgccgta gttcgactgg
      301 cccaccatgc ccaggattcc cgaggtcgag gaggtgttga cgatccgccc ggacgtctcc
      361 tcgccggcct tggcccgctc ccgccagtgc gcgcacgcct cgcgggtgac caggaagtgg
      421 ccgcgcaggt ggaccttgat cacggcgtcc cactcctcga ccgactgatt gaagatcatc
      481 cggtcccgca ggatgccggc gttgttcacc accgcgtcga gccgccccca ggcgtcgagc
      541 gccgcgccga tcagcgacgt cgcgccctcc ggctcggaga tgtcctcgaa gtgggccacc
      601 gcctcgccgc cggcggcccg gatctcctcg accacctggg ccgccggccg ggcgtcggcg
      661 ccggtgccgt cccactcgcc gccgaggtcg ttcaccacga cacgggcgcc ctcggccgcg
      721 aacagcaggg cctccccgcg cccgatcccg cgcccggccc ccgtgatcac cgcgaccttg
      781 ccgtcgagca tccccatcgg ccgtctcctc ccgaaacgaa gccataagga taggatcggg
      841 ccatgacggc aggcgcgtcg ccggccgcgt ggcacgcgga cccgtacggc cggcacgagc
      901 tgcgctactg ggacggcgtc cgctggaccg agcacgtctc cgacagcggc cggcaatcgc
      961 tcgactatcc cgacgccgag cgacaggggg tgccgacggt ggcccgctcg accgcgcgga
     1021 tcaccaggga cgtcgctcgc gcgggcgtcg cggccgtcgg tgacggcggc ggttccgtgt
     1081 tcaccgaacc ggtgctcgtg gtgaaccaga aggcgaagct catggagctg accgcgcagt
     1141 acgccgtctt cgaccggaac ggcacgcaga tcggcggcgt gcgccaggtg gggcagagcg
     1201 ccgggcgcaa ggtgctgcgc gcgctgacct cgctcgacca gttcttgacc caccgcctgg
     1261 agatcgtgga catgtcgggc gccgtgctgc tgcgcctggt gcgcccggcg aaggtgatga
     1321 agtcgcgcat cctggtccag gacccgcacg ggaacgaggt cggtcagatc gtgcagcaga
     1381 acgtgatcgg caagatccgg ttctcgctcg aggccggggg cgccgcggtc gggagcctga
     1441 acgccgagaa ctggcgggcc tggaacttct cgatccgcga cgccgagggg gccgaggtcg
     1501 cccggatcac gaagacctgg gagggcctgg ccaagacgat gttcaccacg gccgacaact
     1561 acgtcgtcga gatcatgcgc cgcctcgagg atcccctgcg cgccctggtg gtcgcctccg
     1621 ccctgtccgt cgacaccgcc ctgaagcagg acgcccgagg cctcggctag cgcttcgagg
     1681 gctcgacacc gtgctcggct tgtgtgacga atccataagt gctacacggt aagtatgacc
     1741 caggtacgta cgacggtggc agaccagcac tacgtgctcg aggcgagcga gatcgagcgg
     1801 cgcctccgcg acgagtcgcc cgagccgatc gcggagcact atgtcgtggt cgccggtcgg
     1861 cgcttccccc cgaagcaggt cattggcctg atcaccggac tcgaccgagc cgacttcaac
     1921 acccatcagg ccaggcgcat cctcagccgg ctcggtttca cgatggggcg gcgaagcaag
     1981 gatgctctcc cctccgggcc ggaccggccg gggccgtacc gaggccgcga ggccgatctt
     2041 ctccggccgt tcatggggag gtgggtcgcc cagcgcgggc tcgagatcct ggtcgacgcc
     2101 gacagcccgc aggaggtcct cgcctggttg gagcgccacc gacagcgagc ggacgccgtg
     2161 ttccgcgttc ccgccgacga gaccgaggcg accggcgtgg cgccggcatg aggttcccct
     2221 tcaaggagct tcccggacgg ggccgcgatt acctccgccc ggcggtgccg gtcaccgtgg
     2281 agggcatctc actcgcaccg cagctgtgcc tgctggacac cggcgcgctc cacaaccggt
     2341 tcggcgcctg ggtggcggaa gccgccggga tcgacctcgg cggcgccgag caacagcgcc
     2401 tcgccgtggg cgggttcgtg acggccgcgc gggccgcgcc cgtccagctg acgctgggcg
     2461 acgtgacctg ggaagccccc acctggttct gcgatccgtg gccactcgcc ttccatctcc
     2521 tcggccagga ggggttcttc cgctggttcg aggtgtgcct gcgcgccggc gcgtacgaga
     2581 tcgaggtcac gccgggggac tgagctgaga cctccaccac cggcaggctg cgatcgtcag
     2641 cgagtcgcca gcgagcggat ggcgcggacc aatgcgacga gcgcgagggc gaagccgggg
     2701 aaggcgaagg cgtatttcgg gatcgccagg gcgtgggcgg cgaccagggc gccgtcaccg
     2761 gtggcctgga gcgagaggac gatcagtgtg ttctccaggg cgtcgacgat cccgccgccc
     2821 gccaggaaca ggatccccag gcgcagccag agcggcgggt ggccgtcccg gccgattgcc
     2881 cggccgaacc gccagagcgc caccgggccg agcagcccgt agaggacgat gaacacgtag
     2941 tcggcggtga agaagtcccg cgcctcctgc gccttcgact gcagttcggc cagaccgctc
     3001 gtctggatgt cctgcgggaa cgtcagcagc gctgtggcga gcgcggtggc caccgcgaac
     3061 agggcgatca cagcccacgc cctccgcgcg cgctcctcgc ggccgaaggc ctcgtgctcc
     3121 acgcgggtct ccgggacggt ctcggccggc atcgcgcgca accctacccc ggcggcagcg
     3181 ccggctttcg gcgccggcgg accgcgggca atccgccggt gcgggagaga tgcaccgcgc
     3241 gccagcagta ccaggcagcg atggttcgcc acgggcggaa gcggtcgccg agcggctcga
     3301 gctccttcgg cttcggtacc tcggccaggc cgtgggcgat ggcccagccg ccgcgcacgc
     3361 cgtagtcgtc aaccggccag acgtcgaggc ggcgcaggtc gaagatcagg aacatctcgg
     3421 ccgtccaccg cccgatcccg cggacctgcg acaggcgcgc cacgatctcg tcgtcgggca
     3481 gcttctcgat cccgtcgagc ggcaccgtgc cgtcggccac cttcgcggcg aggtccttga
     3541 tcgaggcgag cttggcgccg gacacgccgg ctccgcggat gcgctcgtcc ggcatcgcca
     3601 gcagtgcctc gggggccagc tcgccgtcga acagcgcgcg gaaccggccg tggatcgccg
     3661 cggcggcggc cccggccagc tgctggtaca tgatcgactc gacgagtgag gcgaagaacg
     3721 ccgactccgt gcgccgcgtc ttcgtcaccc tgaacgggcc ggccagctcg atgatccgcc
     3781 cgagcttcgg gtcgcggagg gcgacctcgg cggtggcggc ggcggccgag aggcgcggca
     3841 cggtcacgcc gcggcggcca gcgcctttat cgcgtcgacc tgcagcgcgc ccgcgatcag
     3901 cgtggtgact cccgcctcgc gccacgccgc cagccggtcg ccgatccgct ccgacgggcc
     3961 gaccagcgag acctcgtcca ccagcgcgtc gggcacggcc ccagccgcct cgagcttctt
     4021 cccggaaagg tacagttcct gtaccttctc ggcttcggcc tcgtacccgt agcgctgcac
     4081 gaggttcgtg tagaagttcc tcccccgcgc ccccatccct ccgacgtaga gcgccaggaa
     4141 cggcttcacc aggtcacggc aagcctgcac gtcgtcgccg gca
//
LOCUS       DASPWO010000406         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_316299, whole genome shotgun sequence.
ACCESSION   DASPWO010000406 DASPWO010000000
VERSION     DASPWO010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316299"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VEO00_09305"
     CDS             complement(<1..313)
                     /locus_tag="VEO00_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58230.1"
                     /translation="MARLYLVADDGEIARRRKLVPPGRLVEAWPDLYAAGQFWIGEAS
                     KTLLDGAGPPLPVRLSLDGAVVPIYYGPRLRDLESLPLEESLQTRVLSARGIAVAWIT
                     FD"
     gene            complement(335..985)
                     /locus_tag="VEO00_09310"
     CDS             complement(335..985)
                     /locus_tag="VEO00_09310"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HYU58231.1"
                     /translation="MTLPLPLRNRLAELILESLHDPEARRGLEAVATVCADPRALTHV
                     GELPARVPRDFFEERAGGLQVKSGWKEYAAELRRRSGRAWRAIRDRPLDPADAPLGTI
                     LAAAALLFDAGLYYEVHELLEPPWMRAGGGAREALQGLIQIAVGFQHLANGNVAGARG
                     LLADGCAKTLGRRLHGLDLDPFARAARGCFEQVASRGANAAREFDWAAVPRFPTRS"
     gene            1282..>2363
                     /gene="glp"
                     /locus_tag="VEO00_09315"
     CDS             1282..>2363
                     /gene="glp"
                     /locus_tag="VEO00_09315"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HYU58232.1"
                     /translation="MISVSDGQARVLAQVTTVAPPELVPVGRCLGRVTAEDVRSSMDV
                     PPTDNSAVDGYAVGCGDIPTGGSRELDVVAELAAGSVFPDVLGPGQALRIMTGAPMPA
                     GADTVYPQEIVIREGRRVVIPPIARAANVRRRGEDVKAGAVVLPAGTVLRPQELGVAA
                     SLGLAQLMVRQRPRVAILSTGDEVAEPGVERKPGQIYDSNRFALRGLAEAAGAQAADH
                     GVVPDLFDELYARLLAAAASADIVLTSGGVSVGDYDLVKAVLQETGGIDFWQVAMQPG
                     RPIAVGRIGTAHFFGLPGNPVACMLTFHLFVRPALWKLGGRRELFGPRFHAVAVAPMR
                     KKVGRREFKRGILTYTGERWEVRTTGP"
BASE COUNT          338 a          858 c          823 g          344 t
ORIGIN      
        1 ggtcgaacgt gatccatgcc acggcgatgc cgcgggctga caggacacgt gtctggagcg
       61 attcctcgag cggcagcgac tccaaatccc gaagccgcgg cccgtaatag atcggtacca
      121 cggcgccgtc gagggacagc ctgaccggca gcggcggccc cgcgccgtcc agcaacgtct
      181 tggacgcctc ccctatccag aactgcccgg cggcatagag gtccggccaa gcttcgacca
      241 agcggcccgg ggggacgagc ttgcgccgtc gggcaatctc tccgtcatcc gcgacgagat
      301 agagccgcgc catcagccga cccgcccgct catgtcagga cctcgtgggg aagcggggca
      361 cggccgccca gtcgaactcg cgggcggcgt tcgcaccgcg ggaggcaacc tgctcgaagc
      421 agccgcgcgc cgcgcgcgcg aacgggtcca gatccagccc gtgcagccgc cgccccagtg
      481 tctttgcgca gccgtccgcg aggagcccgc gggcaccggc cacgttgcca ttggcgagat
      541 gctgaaagcc caccgcgatc tggatcagtc cctggagcgc ctcgcgggcg ccgccgccgg
      601 cccgcatcca gggcggttcc agcagctcgt gtacctcgta gtagaggccg gcgtcgaaga
      661 gcagcgcggc ggcggccagg atcgtcccga gcggcgcgtc ggcgggatcc agcggtcggt
      721 cgcggatcgc ccgccacgcg cgtccggagc gccggcgcag ctcggccgcg tattccttcc
      781 agccgctctt gacctggagc cctccagctc gctcctcgaa gaaatctcgc ggaacgcgcg
      841 ccgggagctc gccgacgtgc gtcagcgccc tcggatcagc gcagacggtg gccaccgctt
      901 cgagccctct gcgggcctcg ggatcgtgaa gggactcgag gatcaactcg gccagccggt
      961 tgcggagcgg aagcggcaac gtcacgtgca ggatacgagc atcgcccgcg cgttgcgcat
     1021 catgtctcct actagggaca tgtcgtggcc atcggtgcag cgtatcgtag cacatcgcgg
     1081 attgcgccaa ccatcgaggc cacgggccac ggtatggtac ggggccaatt cgcgtcgcca
     1141 tactaccgtt ctcggtcacc ggcgggcttc cggtcatgcc gtcggggctc ctccgcgctc
     1201 aaccgcacgc ctcgcgtcgc cggctccctc gggcctcgtc tggctcgcaa cttggcccag
     1261 cacccacggt ataatgccgt catgatctcg gtgagcgacg gccaggcccg ggtcctggcc
     1321 caggtcacca ccgtggcgcc acccgagctc gtccccgtcg gccgctgcct cggccgcgtc
     1381 accgccgaag acgtccgctc ctccatggac gtgccgccca ccgacaactc ggccgtggac
     1441 ggctacgcgg tcggctgcgg ggacattccc acgggcggca gccgcgagct cgacgtggtg
     1501 gcggagttgg ccgcgggctc cgtctttccg gacgtgctcg ggccgggtca agcgcttcgc
     1561 atcatgacgg gcgcgccgat gccggccggc gccgacacgg tctaccccca ggagatcgtg
     1621 atccgcgagg ggcgccgggt cgtcatcccg cccatcgcgc gagccgccaa cgtgcgccgg
     1681 cggggcgagg acgtgaaggc tggcgccgtt gtcttgccgg ccggcaccgt gctccggccc
     1741 caggaactgg gcgtcgcggc gtcgctcggc ctggctcagc tgatggtgag gcagcggccg
     1801 cgtgtggcca ttctctccac gggtgacgag gtggccgaac ccggcgtcga gcgcaagccg
     1861 gggcagatct atgactccaa ccgcttcgcc ctacggggcc tcgcggaggc ggcgggggcg
     1921 caggccgccg accacggcgt cgtccccgat ctcttcgacg agctctacgc gcgcctcctg
     1981 gcggcggccg cgagcgccga catcgtgctc acctcgggcg gcgtctcggt cggcgactac
     2041 gatctcgtca aggccgtgct ccaggagacg ggtggcatcg acttctggca ggtggccatg
     2101 cagccgggac ggccgatcgc ggtgggacgc atcgggaccg cacatttctt cggcctcccc
     2161 ggcaacccgg tggcgtgcat gctcaccttc catctgttcg tgcgcccggc cctctggaag
     2221 ctcggcggcc gccgcgagct gttcggcccg cgcttccacg cggtggcggt ggcgcccatg
     2281 cggaagaagg tcgggcgtcg cgagttcaag cgcggaatcc tgacatacac gggcgagcga
     2341 tgggaggtca ggaccaccgg tcc
//
LOCUS       DASPWO010000407         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_651593, whole genome shotgun sequence.
ACCESSION   DASPWO010000407 DASPWO010000000
VERSION     DASPWO010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651593"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VEO00_09320"
     CDS             complement(<1..202)
                     /locus_tag="VEO00_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58233.1"
                     /translation="MRHEPRAARTRAVAAVVAGLVLVAASCAPGPRPPGRATPSEFRP
                     EGRILALGGLRGQSLLDVTLPSG"
     gene            complement(192..>2822)
                     /locus_tag="VEO00_09325"
     CDS             complement(192..>2822)
                     /locus_tag="VEO00_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58234.1"
                     /translation="GSRELPVLAQVPVQLLRFPALLVALVAIAALLAAAASSSGLFLS
                     SAADGAVAIDLSANRHRPALTVTQFGSVTASFLAPRSRALTGAVAGLLGLGPLVVTVT
                     GLQVEASSSGGSGVPAQVVARTGFRSHVRAVAGSGTGLWVPESLARRISLRPGDELMV
                     AWGKADATVPVGGVYRDLPSGRLPEFWQPVATRLSSANGAALPALLGEPDAVFAALAP
                     HAPSARLDWDFPLEVSALSLQQAQVLAAGLTAVTDGLASPNIAPGSAFRSEGQGAAPY
                     SSTPLPELVRGATELEGSIRTTSGSVAVAGIVIAMVGMAAAGAYAVRRRRVEASLLSA
                     RGMSPVLQGLRAVVESALPAAAGGALGWGIALALVRLLGPSSLIDPAAREAAASSAIR
                     AVAVGLLLYGLGAAFAARRELEEVTRRTSRILSRAPWEAIALVLAGASLYEILTRPAG
                     EPSSGPDRLLLLFPMLFVGGLAGLTVRLLPRVLDRMRRRVTARRMPAYLAVRRLASAP
                     RVALLLVAGSSVAVGVLAYAGVLSETIARTAGAKAAVLVGGDVAVPLPPGPGGPPPDR
                     PPPGTPGPSTVVLRLPPATLVPRTPVDVLAVDARTFEAVARWNGHFGGPSLHEILAAL
                     SAPAGGRLPVLLAGGSLSRESTLRVAGQSVPVTVVRQTAAFPGMTTSGLPLVVAGRRA
                     LFAASPSLATLNGTAAELWVRGAPGTILRLLAGAGIDTSSARTASKVRAEPSLLSLSW
                     TLGYLLALGVAAAIVVVAGLLLYLQSRQAANDLSFALGRRMGLSAGTQRRAVTLELGE
                     ILGIAFVLGTGLAVAAAALVRAKLDLLPGVPGPTIFAVPWLLLGATAAGFLLVAIAGA
                     RLVGRRARRTNVAEVLRLAT"
BASE COUNT          433 a         1066 c         1084 g          239 t
ORIGIN      
        1 tgccggaggg cagcgtgacg tcgagcaggc tctggccacg gaggcccccc agggcgagga
       61 tgcggccctc gggccggaac tccgaggggg tcgcccggcc ggggggccgg ggcccgggcg
      121 cgcaagaggc cgcgaccagg accagcccgg cgacgacggc ggcgaccgcg cgggtacggg
      181 ccgctcgggg ctcatgtcgc aaggcgcagc acctccgcga cgttcgtcct gcgagcgcgg
      241 cgccccacga ggcgcgcgcc ggctatggcg accaggagga acccggcagc cgtcgccccg
      301 agcaggagcc agggcaccgc gaagatggtg ggccccggga cgccgggcag caggtcgagc
      361 ttcgcacgca cgagcgcggc cgccgcgacc gcgagcccgg tccccaggac gaacgcgatc
      421 cccaggatct cgccgagctc cagggtcacc gcccgccgct gggtcccggc cgaaaggccc
      481 atccgccggc ccagcgcgaa cgacaggtcg ttcgccgcct gccgcgactg gaggtacagg
      541 agcaacccgg cgacgaccac gatggccgcg gcgacgccga gcgcgagcag gtaccccaac
      601 gtccaggaga gcgagagcag cgagggctcg gcccggacct tcgacgcggt ccgggcggag
      661 ctcgtgtcga tgccagcgcc ggccagcagg cgaaggatcg tgccgggggc gccgcggacc
      721 cacagctcgg cggcggtccc gttcagcgtc gccaagctcg gcgaggcggc gaaaagggcc
      781 cgccggcccg cgacgacgag gggcagcccc gacgtcgtca taccggggaa agccgccgtc
      841 tgccggacca cggtgacggg gacagactgc ccggccacgc ggagcgtgga ttcccgtgag
      901 agcgacccgc cggccaggag caccggcaga cggcccccgg ccggtgccga gagcgcggcc
      961 aggatctcgt ggagcgacgg gcccccgaag tggccgttcc accgcgccac ggcctcgaac
     1021 gtccgggcgt ccaccgccag cacgtcgacc ggggtccggg gcaccagggt cgccggcgga
     1081 agacgcagga cgaccgtcga ggggccgggc gtccccggcg ggggccggtc cggcggcggg
     1141 ccgcccggcc cgggcggaag gggcaccgcc acgtcgcctc ccaccaggac cgccgccttg
     1201 gcgccggcgg tccgcgcgat tgtctccgac aggaccccgg cataggcgag gacccccacg
     1261 gccacggacg agcccgccac cagcagcagc gcgacgcggg gcgcggatgc cagccgccgc
     1321 acggcgaggt aagcgggcat cctgcgagcg gtgacgcgcc ggcgcatgcg gtcgagcacg
     1381 cgcggtagga ggcggaccgt cagcccggcg agccccccga cgaacagcat cgggaacagc
     1441 agcagcagcc gatcggggcc cgaggagggc tcgccggccg gcctggtcag gatctcgtac
     1501 agcgacgcac cggccagtac gagcgcgatc gcctcccagg gggcgcggct caggatgcgg
     1561 gaggtgcgtc gagtgacctc ctccagttcg cggcgcgcgg cgaacgctgc ccccaggccg
     1621 tacagcagca gccccacggc cacggcccgg atcgcggacg acgccgcggc ctcgcgagcc
     1681 gctgggtcga tcagtgagga cggccccagg aggcggacca gcgcgagtgc gatgccccat
     1741 ccgagcgcgc ctccggcagc ggccggaagc gcggactcga ccacggcgcg cagcccctgc
     1801 aatacggggc tcatcccgcg ggccgacagc agcgaggcct ccacccgccg ccgccggacc
     1861 gcgtacgcgc ccgccgcggc catccccacc atcgcgatca cgatgccggc caccgccaca
     1921 cttcccgagg tggtccggat cgagccctcc agctcggtgg cccctcgcac gagctcgggt
     1981 agcggggtgg aggagtaggg agccgcgccc tgtccttcgc tccggaacgc ggagccgggc
     2041 gcgatgttcg ggctggccag cccgtcggtc accgcggtca gccccgccgc caggacctgc
     2101 gcctgctgga gcgagagggc ggagacctcg agggggaagt cccaatccag ccgggccgag
     2161 ggcgcgtgcg gcgccagcgc cgcgaagacg gcgtccggct cgcccagcag ggcaggcaga
     2221 gcggcgccgt tcgcgctcga cagccgagtc gcgacgggct gccagaactc cggcagccgc
     2281 ccggaaggga ggtcccggta gacgccgccc accgggaccg tggcgtcggc tttaccccac
     2341 gcgaccataa gctcgtctcc gggccgcagc gagatgcgtc gcgccaggga ctccgggacc
     2401 cacaggccgg ttccacttcc ggcgaccgcg cggacgtgcg accggaaccc ggtccgggcc
     2461 accacctggg cggggactcc gctgcccccg gacgaggacg cctccacctg caggcccgtc
     2521 accgtgacga cgagcggccc cagtccgagg agccccgcga ccgcaccggt cagcgccctg
     2581 gacctcgggg ccaggaacga agccgtcacg gagccgaact gcgtgacggt gagcgccggc
     2641 cggtggcggt tcgccgagag gtcgatcgcg accgccccgt cggccgcgga ggacaggaac
     2701 agcccgctgg acgatgcggc ggcggccagg agcgccgcga tggcgacgag ggcgacgagc
     2761 agcgccggga accggaggag ctggaccggg acctgcgcga gcacggggag ctcgcggctt
     2821 cc
//
LOCUS       DASPWO010000408         9981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1243795, whole genome shotgun sequence.
ACCESSION   DASPWO010000408 DASPWO010000000
VERSION     DASPWO010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9981
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243795"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VEO00_09330"
     CDS             <1..684
                     /locus_tag="VEO00_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58235.1"
                     /translation="VETKGVYIQDVVLPEALVEVLSTREIAVQRRQTFAREQEAQVAR
                     IDVEKAKGTADQQAELARAQVNVDVQKNRAAARTAEGEGEASFTRITGQGEAARVEAV
                     GLARAKAIEARGVAQAVGYEEQVKALGSAATALVAISQAVAEGRIKVVPDVLVTGGEG
                     GGLDGLAATLMERLRPKAGDGARERLPERGARTTANVRRGGSRAARAPSPPPTAPEAP
                     AAPPEPPPG"
     gene            complement(728..1759)
                     /locus_tag="VEO00_09335"
     CDS             complement(728..1759)
                     /locus_tag="VEO00_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYU58236.1"
                     /translation="MTVARVRLPMLATVAVLVALAAVPVRADTEDDLRRAKERVAKVQ
                     AELDAATAAYQQAFGQFVATQDRIVETRERMARTRQRLKRLRASLAARARQAYQQGAA
                     GTLELLLSSNSFAEFADRAAFLDRMSQGESDLILRAQVTRESLTRQRDDLDALAAEQA
                     STTKVLSQQQAAIDAKFGELLAIEDDLEQKLASERAAAAALTRIAPGTVIRGGALQAC
                     PVAGPRSYYDDFGAPRPGGRTHQGNDILAPRGTPVVAAHDGAVSMHPNDLGGNALYVT
                     RSNGDYTYYAHLDSYVGGARTVSAGEQIGTVGNTGNASGGPTHLHFEYHPGGGAAVDP
                     YPYLLAVCG"
     gene            complement(1894..3420)
                     /locus_tag="VEO00_09340"
     CDS             complement(1894..3420)
                     /locus_tag="VEO00_09340"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HYU58237.1"
                     /translation="MNLAALARENVEKFGEYAQVHFEGREYLNTEAVADADRMANVFH
                     ELGVRPGDRVAVMLPNSLEVYQSYGGILSAGAVVVPIVFLLAVPEVRHIVADCTPKVL
                     VTSPEFHPNVLQAIEGLPEPPAMLVTGDAPDGARSFDALRDAATARFDLVDRGEDDVA
                     VIMYTGGTTGRPKGVALSQRNIHWNATTIADTVGIHAGDVGLSALPVAHLFGMISSVT
                     AQVIGVRGILLRWFTADGVLQAIQDHRVQFAPMVPTMMTYLLQEPSVEDFDLSSLEIV
                     FASAAPVPIELAEAFEKRFHCEVVEAYGQTEAGPGITVERPGTRKKAGSAGLPFEGCE
                     VRIEDDEHDVLPAGQLGEVCARGPGVMLGYYNLPDATADTLKHGWLHTGDMGFVDRDG
                     YLFVTERKKDLIIRGGFNVYPRDVEELLNRHPAVAEAAVVGMPDPAMGEEVVAFVVRK
                     PGAEVTEEEVLAFARQGLARYKVPKEIHFRAALPKSPIGKVLKKDLRAELAPPPSGEA
                     "
     gene            complement(3434..4630)
                     /locus_tag="VEO00_09345"
     CDS             complement(3434..4630)
                     /locus_tag="VEO00_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU58238.1"
                     /translation="MDLAALPTRRLAEIPDRGLFTEDHRTLRHAVRSWVEGRLAPHAE
                     EWEREGTFPVRDIFAEAGAMGLFGAKVEEAYGGTGPDLVADAVITEESVRCNSGGVAA
                     ALGAHKDLGPYYVYRFGTEEQRRWWVTRAVAGEWIGALAVTEPDAGSDVAAIRTRAVP
                     DGDGWRISGTKTFITNGPIADFVVVAAKTDPDAGAHGISLFIVGPEDVMSGRVERRRI
                     DTLGWRTSHTGELVFQDVPVTRANLLGEENRGFHHIMANFQWERMVMALAAVAAAERT
                     LELAIAYARDRSAFGRPVAKFQVWRHRFADLATDIEAARSLAYHALRSIVAGEDATRE
                     VSMAKWLATELDWRVADEAVQVHGGYGYVMEYPVQRAWRDARLGPIGGGTTEIMKEII
                     GRLMGA"
     gene            complement(4635..5783)
                     /locus_tag="VEO00_09350"
     CDS             complement(4635..5783)
                     /locus_tag="VEO00_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU58239.1"
                     /translation="MARHPLFTDELEALRASMRAFVEAELAPHALDWEREEDFPDWVF
                     KRMGDLGFLGVRYPEAVGGQGGDWGHTIVVSEELARIGSAGVGMAIAVQTDMATPPIL
                     RFGTPEQIERYLAPAIRGEKIACLGITEPNAGSDVANIETTAVRDGDEWVINGRKIFI
                     TNGARADFCLLVTRTDKPAGYEGFSLFLVDTDTPGWSVAKKLHKVGMRSSDTAEIAID
                     DVRVPASALLGEEGQGFTQIMWELQGERLIGAAGSVAGAQLAMEKTLEYAKARRAFGK
                     PIAGFQSIRHAFAEMATELEAARQLTYDVALAWERGQYPVKEISMVKLYGGIVVNRVM
                     NRCLQIWGGAGYATDGFIERAWRDARVIRIGGGTDEVMREVVAKTMGL"
     gene            complement(6055..6309)
                     /locus_tag="VEO00_09355"
     CDS             complement(6055..6309)
                     /locus_tag="VEO00_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58240.1"
                     /translation="MVVGLVGLALARYGKGRFEAVERRIDRLEQRMDARFSQVDARID
                     GVEQSLGGRIDGVERSIDTMRSDLTYVALAVGARPRDRNA"
     gene            complement(6436..7596)
                     /gene="sat"
                     /locus_tag="VEO00_09360"
     CDS             complement(6436..7596)
                     /gene="sat"
                     /locus_tag="VEO00_09360"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006620327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HYU58241.1"
                     /translation="MTDDIIAPHGGTLVDLVMPNDRAEDAAAEAEHYPKLRLGQREQA
                     DLEMLAMGALSPLTGFVGEKDYRSILEDMRLSNGLPWTIPVTLAVDDEELKAIGRADA
                     VALQSGGSTIAVLEVSDTYERDREVEAQSVFGTIDAAHPGVKHLHESGRFLVAGTVRV
                     LHLPRHTRFTNYRMTPAQTRRAFAERGWKRVVGFQTRNPVHRAHEYIQKCALEITDGL
                     LLHPLVGATKEDDVPADVRMRCYEVLFEAYYPQNRAMLAVFPANMRYAGPKEAIFHAI
                     CRKNYGCTHFIVGRDHAGVGDFYGTYEAQEIFDRFDADELGITPLRFEHSFWCNRCEG
                     MASGKTCPHGEEHRVALSGTKVREMLRAGQMPPPEFSRPEVAKILIDAMSAG"
     gene            7775..8515
                     /locus_tag="VEO00_09365"
     CDS             7775..8515
                     /locus_tag="VEO00_09365"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HYU58242.1"
                     /translation="MKALLPEDLVRKTNARFEDATPQEIIRWALQDSGLDDVAVASSF
                     QMEDTVVMEMAVRARADVPILFLETGFHFAETLAFKDRLTQRLGLNVVDLVGDYTVEA
                     QNAAFGDKLWERDPTRCCELNKVIPLNRALRQLDGWVTGMRRDSAPTRANAPIVDQYE
                     LEPGKTLVKFNPLANWTRRDVVAYLKEHDLPRLPLYELGYAQIGCAPCTRMVFPGEDE
                     RAGRWAGADKVECGIHTMETAREAALQK"
     gene            complement(8545..9231)
                     /locus_tag="VEO00_09370"
     CDS             complement(8545..9231)
                     /locus_tag="VEO00_09370"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HYU58243.1"
                     /translation="MRRPLVIIIVAVLAAVAADFVLRFWTQSWVKSEIASDLDARGGA
                     TVSAGGFPFALQALRGHVNSASVTAEDFTVDGLRVERLDMDVKLVKFPLLTMLTRGDA
                     TLTISRGRGTATVTDDDVTAWLSFQDYPGRVTFLADGNVQVDVMANVGGQQLEVTATG
                     PVRIDQEQLIFEPKSASSGGTEVPLEAVAFAFDLPPMFEGFTYRQIEVRGGTAELGVG
                     VRDGRLAIGD"
     gene            complement(9242..>9981)
                     /locus_tag="VEO00_09375"
     CDS             complement(9242..>9981)
                     /locus_tag="VEO00_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYU58244.1"
                     /translation="PLLGSRLGHGPVALVLCHGFLGWHRKARQVRFAEALARWFTVYA
                     FDFRGHGRSGGLCTLGDTELYDVDAVVRLARADGFERVVTVGASMGGVAAVRQAALRG
                     GVDAVVAISTPAGWDGHVSPSVDRMRRLTSTERGRRIARRLGVRMSAEWDDPASPEEV
                     APLVSPTPLFVVHGRDDHFFDEEQAWRLYRAAEQPKRLLLAGRFGHAEDGFSPGLARM
                     LAVRIHEALDEPVPVGAERRVPVGPGC"
BASE COUNT         1407 a         3678 c         3487 g         1409 t
ORIGIN      
        1 gtcgagacga agggcgtcta catccaggac gtcgtgctcc ccgaggcgct cgttgaggtg
       61 ctctcgacgc gggagatcgc cgtgcagcgc cgccagacct tcgcccggga gcaggaggcg
      121 caggtggccc ggatcgacgt ggagaaggcg aagggcacgg ccgaccagca ggcggagctg
      181 gcgcgggcgc aggtgaacgt ggacgtgcag aagaaccgcg ccgcggcgcg caccgcggag
      241 ggcgagggcg aggcgtcgtt cacgcgcatc accggccagg gcgaggcggc gcgcgtcgag
      301 gcggtcggcc tggcccgggc gaaggcgatc gaggcccgcg gcgttgccca ggccgtcggc
      361 tacgaggagc aggtgaaggc gctcggctcc gcggccaccg cgctggttgc catctcccag
      421 gccgtcgccg agggacggat caaggtggtc cccgacgtgc tggtcaccgg cggcgagggc
      481 ggcgggctgg acggtctcgc cgcgacgctg atggagcggc tccgcccgaa ggccggcgac
      541 ggggccaggg agcgcctgcc cgagcgcggc gccaggacca cggccaacgt tcggcgcggg
      601 ggctcgcgcg cggcgcgggc gccgtccccg ccgccgaccg cgcccgaggc gccggcagcg
      661 cccccggagc cgccgccggg ctgagcgcgg gggctcgcgc gcgagcggga gcgggagcgg
      721 gagcggccta cccgcagacg gccagcaggt aggggtaggg atcgacggcc gcgccgccgc
      781 cggggtggta ctcgaagtgc aggtgggtcg ggccgccgct ggcgttcccg gtgttcccga
      841 cggtcccgat ctgctcgccg gccgacacgg tgcgcgcacc ccccacgtac gaatcgaggt
      901 gcgcgtagta cgtgtagtcc ccgttcgagc gggtgacgta gagcgcgttg cccccgaggt
      961 cgttcggatg catgctgacc gcgccgtcgt gcgccgccac caccggggtc ccgcggggcg
     1021 cgaggatatc gttgccctga tgcgtccggc cgccgggccg cggcgccccg aagtcgtcgt
     1081 agtacgagcg ggggccggcc accgggcagg cctgcagggc gccgccccgg atcacggtcc
     1141 ccggcgcgat gcgcgtgagc gcggcggcgg ccgcgcgctc cgacgcgagc ttctgctcga
     1201 ggtcgtcctc gatcgccagc agctccccga acttcgcgtc gatcgccgcc tgctgttggg
     1261 acaggacctt ggtggtggac gcctgctcgg cggcgagcgc gtcgaggtcg tcgcgctggc
     1321 gggtcaggct ctcccgcgtc acctgcgccc gcaggatcag gtccgactcc ccctgggaca
     1381 tccgatccag gaaggccgcc cggtcggcga actcggcgaa cgagttcgag gagagcagca
     1441 gctcgagcgt gccggccgcg ccctgctggt aggcctgccg cgcccgcgcc gcgagtgagg
     1501 cgcggagccg cttcagccgc tggcgcgtgc gggccatccg ctcgcgcgtc tcgacgatgc
     1561 ggtcctgggt ggcgacgaac tgaccgaacg cctgctggta cgccgcggtg gccgcgtcga
     1621 gctcggcctg caccttcgcc acgcgctcct tggcccgccg gaggtcgtcc tcggtgtcgg
     1681 cgcgcaccgg tacggcggcg agcgcgacca gcaccgccac cgtggcgagc atgggaagtc
     1741 tgactctggc gacggtcacc ggttaccccg aacccccacg tgcggtaggc cgcgcgaagt
     1801 tccgcagcac cctaccgggc gatcccggta gtggcaaacg tcgtgaccgg gacagaacgg
     1861 gattgctccc ggactgtccg gttcgaccgg ctctcacgct tcgccggacg ggggaggggc
     1921 cagctcggcc cgcaggtcct tcttcaggac cttgccgatc gggctcttcg ggagggccgc
     1981 ccggaagtgg atctccttcg ggaccttgta cctggccagg ccctggcgcg cgaaggcgag
     2041 cacctcctcc tcggtcacct cggcccccgg cttcctgacg acgaacgcga cgacctcctc
     2101 ccccatcgcc gggtcgggca tgccgaccac ggcggcctcg gccaccgcgg ggtgccggtt
     2161 cagcagctcc tcgacgtcgc gcgggtagac gttgaagccg ccgcggatga tcaggtcctt
     2221 cttgcgctcg gtgacgaaga ggtagccgtc cctgtccacg aagcccatgt cgccggtgtg
     2281 cagccacccg tgcttcagcg tgtcggccgt cgcgtccggc aggttgtagt acccgagcat
     2341 cacgccggga ccgcgggcgc acacctcgcc gagttgcccg gccggcagca cgtcgtgctc
     2401 gtcgtcctcg atccgcacct cacagccctc gaacggcagt ccggccgacc ccgccttctt
     2461 ccgcgtgccg ggccgctcga cggtgatccc cggcccggcc tctgtctgcc cgtaggcctc
     2521 gaccacctcg cagtggaacc gcttctcgaa cgcctcggcc agctcgatcg gcaccggcgc
     2581 cgcggaggcg aacacgatct ccagcgagga caggtcgaag tcctcgacgc tcggctcctg
     2641 cagcaggtag gtcatcatcg tcgggaccat cggcgcgaac tgaacgcggt ggtcctggat
     2701 cgcctgcagc acgccgtcgg cggtgaacca gcgcagcagg atcccgcgca cgccgatcac
     2761 ctgggcggtg accgaggaga tcatcccgaa caggtgggcg accggcagcg ccgagagccc
     2821 gacgtcgccg gcatggatcc cgaccgtgtc cgcgatcgtc gtggcgttcc agtggatgtt
     2881 gcgctggctg agcgcgaccc ccttcggccg gccggtcgtc ccgcccgtgt acatgatcac
     2941 ggccacgtcg tcctcgcccc gatccacgag gtcgaaccgg gccgtggcgg cgtcgcgcaa
     3001 cgcgtcgaac gaccgagccc cgtccggagc gtctccggtg acgagcatcg ccggcggctc
     3061 gggcaggccc tcgatcgcct gcaggacgtt cgggtggaac tccgggctgg tcaccaggac
     3121 cttcggcgtg cagtcggcca cgatgtggcg cacctcgggc acggccagca ggaagacgat
     3181 cggcacgacg accgccccgg ccgagaggat cccgccgtag gactggtaga cctcgagcga
     3241 gttcgggagc atcaccgcca cgcgatcgcc cgggcggacg cccagctcgt ggaacacgtt
     3301 cgccatccgg tcggcgtccg ccacggcctc ggtgttcagg tactcgcgcc cctcgaagtg
     3361 aacctgcgcg tactccccga acttctcgac gttctcacgc gccagcgcgg ccaggttcat
     3421 cgccaccctc cccctaggcg cccatcaggc ggccgatgat ctccttcatg atctcggtgg
     3481 tgccgccgcc gatcgggccg aggcgggcgt cgcgccaggc ccgctgcacc gggtactcca
     3541 tcacgtagcc gtagccgccg tgcacctgga cggcctcgtc ggcgacgcgc cagtcgagct
     3601 cggtagccag ccacttcgcc atcgacacct cgcgggtggc gtcctcgccg gcgacgatcg
     3661 aacgcagcgc gtgataggcc aggctgcgcg cggcctcgat gtcggtggcc aggtcggcga
     3721 aacgatgccg ccagacctgg aacttcgcca ccggccggcc gaacgccgag cggtcccggg
     3781 cgtaggcgat cgcaagctcg agcgtccgct ccgccgcggc caccgcggcc agcgccatca
     3841 ccatccgttc ccactggaag ttggccatga tgtggtggaa gccgcggttc tcctcgccca
     3901 gcaggttcgc ccgggtcacc gggacgtcct ggaagacgag ctcgccggtg tgcgaggtcc
     3961 gccagccgag ggtgtcgatg cggcggcgct ccacccggcc gctcatgacg tcctcggggc
     4021 cgacgatgaa cagcgagatg ccgtgggcgc cggcgtccgg atcggtcttc gccgcgacca
     4081 ccacgaagtc cgcgatcggc ccgttcgtga tgaaggtctt ggtgccgctg atccgccacc
     4141 cgtcgccgtc cgggaccgcg cgggtccgga tcgccgcaac gtccgacccg gcgtcgggct
     4201 cggtcaccgc cagcgcgccg atccactcgc cggccaccgc ccgcgtcacc caccagcgcc
     4261 tctgctcctc ggtcccgaag cgatacacgt agtacgggcc gaggtccttg tgggcgccca
     4321 gggcggccgc gaccccaccc gagttgcagc ggaccgactc ctccgtgatc accgcgtccg
     4381 cgacgaggtc cgggcccgtc ccgccgtagg cctcctcgac cttggcgccg aacagcccca
     4441 tcgcgccggc ctcggcgaag atgtcccgca cggggaacgt cccctcgcgc tcccactcct
     4501 ccgcgtgcgg cgccagccgg ccctccaccc aggaccgcac ggcgtgccgc agcgtgcggt
     4561 ggtcctcggt gaacaggccg cggtccggga tctcggccag tcgtcgcgtc ggcagggcgg
     4621 ccaggtccat cccctcagag ccccatcgtc ttcgcgacga cctcgcgcat gacctcgtcg
     4681 gtacctcccc cgatgcggat cacccgcgcg tcgcgccagg cgcgctcgat gaacccgtcg
     4741 gtggcgtagc cggcgccgcc ccaaatctgc aggcagcggt tcatgacgcg gttgaccacg
     4801 atcccgccgt acagcttgac catcgagatc tccttgaccg ggtactggcc gcgctcccag
     4861 gccagcgcca cgtcgtaggt cagctgccgc gccgcctcca gctcggtggc catctccgcg
     4921 aacgcgtggc ggatcgactg gaacccggcg atcggcttcc cgaacgcgcg ccgcgccttc
     4981 gcgtactcga gcgtcttctc catggcgagc tgcgcgccgg cgaccgagcc tgcggcgccg
     5041 atcaggcgct ccccctgcag ctcccacatg atctgggtga agccctggcc ctcctcgccg
     5101 agcagcgcgc tcgccggcac gcggacgtcg tcgatggcga tctcggccgt gtcggagctg
     5161 cgcatcccga ccttgtggag cttcttcgcc acgctccacc ccggcgtgtc ggtgtcgacc
     5221 aggaagagcg agaacccctc gtagccggcc ggcttgtcgg tgcgcgtcac cagcagacag
     5281 aagtcggcgc gtgcgccgtt cgtgatgaag atcttccggc cgttgatcac ccactcgtcg
     5341 ccgtcgcgga cggcggtggt ctcgatgttc gcgacgtcgg agcccgcgtt cggctcggtg
     5401 atcccgaggc acgcgatctt ctcgccgcgg atcgcggggg ccaggtagcg ctcgatctgc
     5461 tctggcgtgc cgaacctcag gatcggcggg gtggccatgt cggtctgcac ggcgatcgcc
     5521 atcccgacgc cggccgagcc gatccgggcc agctcctcgg agacgacgat cgtgtggccc
     5581 cagtccccgc cctggccgcc gaccgcctcc gggtagcgca ccccaaggaa gccgaggtcg
     5641 cccatccgct tgaagaccca gtcggggaag tcctcctcgc gctcccagtc gagggcgtgc
     5701 ggcgccagct ccgcctcgac gaacgcccgc atgctcgccc gcagcgcctc gagctcgtcc
     5761 gtgaacagcg gatgccgggc catggcgcga agtttacgct tcggtaggtt cggcccgcgt
     5821 cacctcggcg agccgctcgg cggcccgggg cggcgcccac gtggcccttc tgcccggcga
     5881 gggtcagggc gtcgcgcaac atgtcctccg accgtcacgg tcttgcgcag ctcggcgggt
     5941 ccagctgcgt cgagcggcgc cgagcagaac ccgcagaagc ggaactcctc ggggttctcc
     6001 cgcccgcagc tcgggcagat caccacgtgc ggagtgtagg cccgccccct ccggtcaggc
     6061 gttcctgtcg cggggtcgag cgcccaccgc cagcgccacg tacgtcaggt cggaccgcat
     6121 cgtgtcgatc gaccgctcga cgccatcgat gcgaccgccc agcgattgct cgacgccgtc
     6181 gatccgggcg tcgacctggg agaaacgggc atccatccgc tgctcgagac gatcgatccg
     6241 ccgctcaacc gcctcgaaac gacccttccc gtaccgggca agggcaaggc ccaccagccc
     6301 gacgaccatc gcgttgatgg cgctctcgac gagatccatc cttctcctct ccgggggagg
     6361 agtacgcgca gcgtagcatc gggcgccgac atcgaaggtg gggccggcag ccggcgcggc
     6421 ggaccggctc cgctcctatc ccgccgacat cgcgtcgatc aggatcttgg cgacctcggg
     6481 tctcgagaac tcgggtgggg gcatctggcc ggcgcggagc atctcgcgga ccttggtgcc
     6541 ggagagggcg acgcggtgct cctcgccgtg cgggcaggtc ttgccgctgg ccatcccctc
     6601 gcagcggttg caccagaacg agtgctcgaa gcgcagcggc gtgatgccga gctcgtccgc
     6661 gtcgaaccgg tcgaagatct cctgcgcctc gtacgtgccg tagaagtcgc cgaccccggc
     6721 gtggtcccgg ccgacgatga agtgcgtgca gccgtagttc ttcctgcaga tcgcgtggaa
     6781 gatggcctcc ttcggtcccg cgtagcgcat gttcgcgggg aacactgcga gcatggcccg
     6841 gttctgcggg tagtaggcct cgaacaggac ctcgtagcag cgcatccgga cgtccgccgg
     6901 gacgtcgtcc tccttggtcg cgccgaccag ggggtggagc agcagcccgt cggtgatctc
     6961 cagcgcgcac ttctggatgt actcgtgcgc ccggtggacg gggttgcgcg tctggaaccc
     7021 gacgacgcgc ttccaccccc gctctgcgaa tgcccgccgc gtctgcgccg gcgtcatgcg
     7081 gtagttcgtg aaccgcgtgt gccggggcag gtgcaggacg cgcaccgtcc cggcgaccag
     7141 gaaccgcccc gattcgtgga ggtgcttcac ccccgggtgc gcggcgtcga tcgtgccgaa
     7201 caccgactgc gcctcgacct cgcggtcccg ctcgtacgtg tcggagacct cgaggacggc
     7261 gatcgtcgag ccgccggact gcagggcgac cgcgtccgcc cgcccgatgg ccttcagctc
     7321 ctcgtcgtcc acggcgagcg tgaccgggat ggtccacggc aggccgttcg acaggcgcat
     7381 gtcctcgagg atcgaccggt agtccttctc gccgacgaac ccggtcagcg gcgacagcgc
     7441 gcccatggcc agcatctcga ggtccgcctg ctcgcgctga ccgagccgca gcttggggta
     7501 gtgctcggcc tccgcggcgg cgtcctccgc ccgatcgttc ggcatgacga ggtccacgag
     7561 cgtgccgccg tggggcgcga tgatgtcgtc ggtcatcggg aatgctcctc cgtggtcggg
     7621 tgttgccctg ttcgaggcga cgcgccggcc agtctatcgg agcctcgaac ggcctccgcg
     7681 gggccacgcc gcccgaggga gtgccccggg accggcgggt atactcggag tgccgctcgc
     7741 ttcgccgggc ggtccaaacg cgaaggagtc accgttgaag gccctcttgc cagaagacct
     7801 cgtccggaag acgaacgccc gcttcgagga cgcaaccccg caggagatca tccgctgggc
     7861 cctgcaggac tccgggctcg acgacgtcgc cgtcgcctcg agcttccaga tggaggacac
     7921 ggtcgtgatg gagatggccg ttcgggcccg agcggatgtg ccgatcctgt tcctcgagac
     7981 cggcttccac ttcgccgaga ccctcgcctt caaggaccgg ttgacccagc gcctcggtct
     8041 gaacgtcgtc gacctggtcg gcgactacac cgtcgaggcg cagaacgccg cgttcggcga
     8101 caagctgtgg gaacgcgacc cgacgaggtg ttgcgagctg aacaaggtca tccccctgaa
     8161 ccgggcgctg cggcagctcg acggatgggt caccgggatg cgccgcgatt ccgccccgac
     8221 gcgggcgaac gcgccgatcg tcgaccagta cgagctggag cccggtaaga cgctcgtcaa
     8281 gttcaacccg ctcgccaact ggacccgccg cgacgtcgtc gcctacctga aggagcacga
     8341 cctcccgagg ctgcccctct acgagctcgg ctacgcccag atcggctgcg cgccgtgcac
     8401 gcggatggtg ttcccgggcg aggacgagcg cgcgggccgg tgggccggcg ccgacaaggt
     8461 ggagtgcggc atccacacca tggagacggc cagggaagcc gccctccaga agtagcgccc
     8521 gtccggctcg gcgtcgctcg ccgcctagtc gccgatcgcc agcctgccgt ctcgcacgcc
     8581 gacgcccagc tcggccgtgc cgccgcgcac ctcgatctgc cggtaggtga agccctcgaa
     8641 catcggcggc aggtcgaagg cgaaggccac cgcctccagc gggacctcgg ttccgcccga
     8701 cgaagcggac ttcggctcga agatcagctg ctcctggtcg atccgcaccg ggccggtcgc
     8761 cgtgacctcc agctgctggc cgccgacatt cgccatgacg tcgacctgca cattgccgtc
     8821 cgccaggaac gtcacccggc cggggtagtc ctggaacgac agccaggcag tgacgtcgtc
     8881 gtcggtcacg gtggcggtcc cccggccacg gctgatcgtg agcgtcgcgt cgcctcgggt
     8941 cagcatggtg agcaacggga acttcaccag cttgacgtcc atgtccaggc gctcaacgcg
     9001 cagcccgtcg accgtgaagt cctccgcggt gacgctggcc gagttgacgt ggccgcgcag
     9061 ggcctgcagg gcgaacggga acccgccggc ggagacggtc gcgccgccgc gcgcgtcgag
     9121 gtcggacgcg atctcgctct tcacccagga ttgcgtccag aagcgcagca cgaagtcggc
     9181 ggccacggcg gcgagcaccg cgacgatgat gatgaccagc gggcgacgca tgccgccgag
     9241 actagcagcc cggtccgacc ggaacgcgac gttccgcgcc gaccgggacc ggctcgtcga
     9301 gcgcctcgtg gatgcggacg gcgagcatcc gggccaggcc gggcgagaac ccgtcctcgg
     9361 cgtgcccgaa cctgccggcc agcagcaggc gcttcggctg ctcggccgct cggtacagcc
     9421 gccaggcctg ctcctcgtcg aagaagtggt catcgcggcc atgcaccacg aagagcggcg
     9481 tggggctgac gagcggggcc acctcctccg ggctggcggg gtcgtcccac tcggccgaca
     9541 tccgcacccc gaggcggcgc gcgatgcgcc ggcctcgctc ggtggaggtc aggcggcgca
     9601 tgcggtccac cgatggcgag acgtggccgt cccagccggc aggagtcgag atggcgacga
     9661 cggcatccac gccgccacgc agcgcggcct ggcggacggc ggccaccccg cccatcgacg
     9721 cccccaccgt cacgacgcgc tcgaagccgt cggcccgcgc cagccggacg accgcgtcga
     9781 cgtcgtacag ctccgtgtcc cccagggtgc agagaccgcc gcttcgcccg tggccgcgga
     9841 agtcgaacgc gtagaccgtg aaccagcgcg ccagcgcctc ggcgaagcgg acctggcggg
     9901 ccttgcggtg ccacccgagg aacccgtggc agaggaccag cgccaccggc ccgtggccga
     9961 ggcgggagcc gaggagcggc a
//
LOCUS       DASPWO010000409         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1160245, whole genome shotgun sequence.
ACCESSION   DASPWO010000409 DASPWO010000000
VERSION     DASPWO010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160245"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VEO00_09380"
     CDS             complement(<1..297)
                     /locus_tag="VEO00_09380"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HYU58245.1"
                     /translation="MAVEVERARRLFTVEEYERMVEAGILTERDRVELIDGEIVEMTP
                     IGDPHSAALAALIALFTERLGRRVLLWPGGSLRLPARSMPQPDLMLLPPRADFYR"
     gene            complement(373..1419)
                     /locus_tag="VEO00_09385"
     CDS             complement(373..1419)
                     /locus_tag="VEO00_09385"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HYU58246.1"
                     /translation="MFVALCCLTAGVPAALAHTGGSTGYASIIISGNTVRYSLTLSPS
                     ALPAAVAEELTRARSGRADSRDRLLGHLRDRLGLTDQGRRCEPAQGFIEAERAEVESV
                     TLVMDFACASTVRDLVIHDDLFDVLGPDHHTLAKVESAGVTRELAFATEAREARLSLG
                     GAHETSSGAGSFLLLGVHHILTGYDHLLFLAALLLRGGRLTSLLKIITAFTLAHSITL
                     ALAVLGFVTIPDRLVEPVIAASIVWVAAENLILREAPSQRWLVSFLFGLVHGFGFASA
                     LTPLALPPWNLAMALLGFNLGVEAGQAVVVGLLLPFLLWMRGAAWEGRLVRTASLAVA
                     LAGGAWLVERLFLA"
     gene            complement(1485..2039)
                     /locus_tag="VEO00_09390"
     CDS             complement(1485..2039)
                     /locus_tag="VEO00_09390"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HYU58247.1"
                     /translation="MRRARGAALLAAAGLFAVGFLVVMAASGRMRPMQNLVKFEAAGL
                     MRETPNHIDRVELTAEGRRLIFTRREPGRWAVAPSARELAAGAVSHLEMSLKFMHVTA
                     PVRVMSREEYQGEALGEYGLDPPRYTVSLHRGGQRVLATSFGGKNPQRVFQYVRVKGL
                     DELYLLPVFVGREWELVAEGVTAP"
     gene            complement(2036..>2689)
                     /locus_tag="VEO00_09395"
     CDS             complement(2036..>2689)
                     /locus_tag="VEO00_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58248.1"
                     /translation="LARLLAKLGLSLEQQVVVDPLDHYSADLESVAVPIYEPHPITDR
                     IALTFYPGIRPITLLLPPPGVTVTPLFESSKESYARTVPPAQERQPVREPAAVTSAPA
                     STPGRRILAVAVEGTRPERPPGFGPTRGAFRVVVVGDGDFASNSFFPYMSNSELALSM
                     VRWLAREERAPKVRAGVPVPPLVLLTRQQMKTIFLAVELLLPLGVIVVGALVWWRRR"
BASE COUNT          459 a          936 c          950 g          344 t
ORIGIN      
        1 acggtagaag tcggcccgcg gcggcaagag catcaggtcg ggctggggca tggaacgcgc
       61 cggcagccgc agcgagcccc caggccacag gaggacgcgc ctcccgaggc gttccgtgaa
      121 gagcgcaatg agggctgcaa gagcggcact atgagggtcg ccgatgggcg tcatctcgac
      181 gatctcgccg tcgatcagct cgacgcggtc gcgctcggtc aggatacccg cctcgaccat
      241 ccgctcgtac tcctccaccg tgaagagccg cctcgcccgc tcgacctcga ctgccatggc
      301 gggtttcatt ctactggggc gtcacaaccg gttgtcaaga cgtatcttcc cagtatcttc
      361 ccggcgacgc ctctacgcga ggaagagccg ctcgacgagc cacgcgcccc cggcgagcgc
      421 cacggcaagc gaggccgtcc gcaccaggcg gccctcccag gccgctccgc gcatccagag
      481 gaggaacggc agcaggagcc ccaccaccac cgcctgaccc gcctccacac ccaggttgaa
      541 gccgaggagc gccatcgcga ggttccacgg cggcagggcc agcggcgtga gggcggaggc
      601 gaagccgaag ccgtggacga ggccgaagag gaagctcacg agccagcgct gcgagggcgc
      661 ctcgcgcagg atcaggttct cggcggcgac ccacacgatg gacgcggcga tcaccggttc
      721 gaccagacga tccgggatcg tcacgaagcc gagcacggcc agtgcgagcg tgatgctgtg
      781 ggcgagggtg aaggcggtga tgatcttgag cagggaggtg agccggccgc cccgcagcag
      841 gagggcggcc aggaacagca ggtggtcgta gccagtcagg atgtgatgga cccccagcag
      901 aaggaagctc ccggcgccac tggaggtttc gtgggcgccg cccagtgaga gccgcgcctc
      961 ccgcgcctcg gtggcgaacg ccagctcgcg ggtgacgccc gcggactcca ccttggccaa
     1021 cgtatggtga tccgggccga ggacgtcgaa gagatcgtcg tgaatgacga ggtcccggac
     1081 ggtggaggcg caggcgaagt ccatcacgag cgtcacgctc tcgacctcgg cgcgctccgc
     1141 ctcgatgaag ccctgagccg gctcgcagcg ccgcccctga tcggtgagcc ccagtctgtc
     1201 gcggaggtgg ccgagcagcc ggtcacggct atccgcgcga ccggaccggg cacgcgtcaa
     1261 ctcctcggcc accgcggccg ggagggcaga gggggagagg gtcaggctgt agcgtacggt
     1321 gttcccgctg atgatgatgg acgcatagcc agtgctgccc ccggtatggg cgagcgcggc
     1381 cggcacgccg gcggtcaggc agcagagagc gacgaacacc accgcggccc gtgcgcgtcg
     1441 cccggggcgg ccaggatgcg ccggaccgcc cgccgccgac acggtcacgg cgccgtcacc
     1501 ccctccgcca ccaactccca ctctcgaccg acgaagaccg ggaggaggta aagctcgtcc
     1561 agacccttga cgcggacgta ttggaaaacc cgctgagggt tcttcccccc gaagctcgtg
     1621 gcgagcaccc tctggccgcc tcggtgcagg gagaccgtgt accgaggcgg atccaggccg
     1681 tactcgccga gcgcctcccc ctggtattcc tcccgagaca tgacgcggac gggcgcggtc
     1741 acgtgcatga acttgagtga catctccagg tgcgagaccg cgccggccgc gagctctcgg
     1801 gcggagggcg ccaccgccca ccgccccggc tcccgccggg tgaagatcaa tcgccgcccc
     1861 tcggctgtga gctcgacgcg gtcgatatga ttcggcgttt cccgcatgag cccggccgcc
     1921 tcgaacttga cgaggttctg catcgggcgc atccggccgg aggctgccat gacgacgagg
     1981 aaccccacgg cgaagagccc tgccgcggcg agcagagctg ctccccgcgc ccgtctcacc
     2041 gtcgtctcca ccagaccagc gcgcccacca cgatcacgcc gagcggcagc aagagctcca
     2101 cggcaaggaa gatggtcttc atctgctgcc tggtcagcag caccagcggc ggcaccggca
     2161 ccccggcccg gaccttggga gcccgctctt cccgcgccag ccagcggacc atcgagagcg
     2221 ccagctcgct gttcgacatg taggggaaga aggagttgct ggcgaagtct ccgtccccga
     2281 ccaccaccac gcggaaggcc ccccgcgtcg ggccgaagcc gggcggcctc tcgggccggg
     2341 ttccttcgac cgccactgcc aggatccgac gccccggcgt cgaggccggc gccgaggtga
     2401 ccgcggcggg ctcccggaca ggctgacgct cctgggccgg cggcaccgtg cgcgcgtagc
     2461 tttccttgct gctctcgaag agcggcgtga cggtcacgcc gggcggaggg agcaggaggg
     2521 tgatcggccg gatgccggga tagaaggtaa gggcgatgcg gtccgtgatc gggtgcggct
     2581 cgtagattgg gacggccacc gattcgaggt cggcggagta gtgatccagg ggatcgacca
     2641 cgacctgctg ctcgaggctc aggccgagct tggccaggag gcgggcgag
//
LOCUS       DASPWO010000410         4114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1160353, whole genome shotgun sequence.
ACCESSION   DASPWO010000410 DASPWO010000000
VERSION     DASPWO010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160353"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VEO00_09400"
     CDS             complement(<1..750)
                     /locus_tag="VEO00_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014673999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HYU58249.1"
                     /translation="MVLAAALLRQVLRRALDTPAVAHWATIFVAITVQAMPFLVLGVA
                     VSAAVAAYVPAGFLPRVLPRRTALAVPAAAAAGVALPGCECGSVPIAGRLVARGAPPA
                     AALAFLLSAPAINPVVLVATAVAFPGRPQVVLARFLASLAAATVVGLIWARTGQGDLP
                     DHVRRRLRSPEGPRGAVLAATAQHDLLQAGGFLIVGAATAATLQTAVPRDILDGVAGA
                     EGFGFGRLDPGDEPVPMTLLEFGQRSVDRDGV"
     gene            894..2774
                     /locus_tag="VEO00_09405"
     CDS             894..2774
                     /locus_tag="VEO00_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HYU58250.1"
                     /translation="MARRWLRGWAVQVSLAVVAIGVVVAAPLAAPDGAATDRTIISGA
                     RSLADTVLVTYATPKDRTTDVHLLAFNDLHGNLEAASLNLYGRFAGGAAYLAKAVKDR
                     QALCGPYQATILAGDNIGASPLQNGLFHEEPITIASNLMHVDFASVGNHEFDKGKDEL
                     LRIQNGGCHADGCTGAPYALANGSSTDVYPGADFQYLSANVVVNATGDTLFPPYAIKK
                     FTSTDGKKFSVGFIGEVLESTPTIVTPAGVAGLTFQHEADAANLAVQELADRGVKIPI
                     LVIHQGGFQSSPAALNGCTGNLANSDILEIASRLDPAIKVIVSAHTHAEYRCTITTGG
                     VTRLITSASSFGRILSDITLTVDPSSKKLMQASATNVLVENALNTPGPNVIRQPDTSK
                     EDPQVQAVVNQYVAAAAPLANQVIGRIQGDLTRTASPQGESALGDVIADAQLAATAPA
                     GLGDAVLAFMNPGGIRADLLVNAISSGGEAPGEVTYGEAFAVQPFGNSLVTKTMTGDM
                     IRRLLQQQFQGCGGTAPATGRFLQISATFSYEQGASAPACADKIGRMWVNGVEVGPTD
                     SFRVTMNNFLATGGDGFTVFNEGTNALGGAQDIDAFVAYFAANEPAGIAVPPLDRIVP
                     KP"
     gene            2843..3058
                     /locus_tag="VEO00_09410"
     CDS             2843..3058
                     /locus_tag="VEO00_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HYU58251.1"
                     /translation="MCRNIVKLRRPEATASDDEVRQAALQFVRKISGYREPSRRNAEA
                     FTAAVDEIAGASRRLLDALPGPSGATR"
     gene            complement(3055..>4114)
                     /locus_tag="VEO00_09415"
     CDS             complement(3055..>4114)
                     /locus_tag="VEO00_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU58252.1"
                     /translation="LLLLDEPTNHLDTDAKAWLMGFLSSYRGALVLVSHDLVLLDRSI
                     TRVLHLDDAALVEYRGTYSLYLEARRLDEARREKTARQQQREIRRLSALADSMRHQTE
                     KRARIAKTLDRRVERMGRRAVVAPRRDRRVTVRFPEPPRSGELVLEASGLAKGYGGPP
                     VFTDVGFTLGRGERLLVMGLNGAGKTSLLRILAGRTEPSAGAFRLGAGVALGYYAQEH
                     EGIRPGLRALDHVREASPAPDQQLRGVMGTFGLTGEVAFQDAGTLSGGEKTKLALAQL
                     VAGRHNLILLDEPTNNLDPPSRSAVGRALAAWSGSMIVVTHDREFVQALAPDCALIMP
                     EGSLDHWSDDLLELVALA"
BASE COUNT          616 a         1501 c         1486 g          511 t
ORIGIN      
        1 gacgccgtcg cggtcgacgg accgctgccc gaactcgagc agggtcatcg ggacgggctc
       61 gtcgccagga tcgagccgac cgaacccgaa cccctcggcc ccggccacgc cgtcgaggat
      121 gtcccgcggg acggccgtct gcagggtggc cgcggtggcc gccccgacga tcaggaaccc
      181 gccggcctgc agcaggtcgt gctgtgccgt ggccgccagc acggcgcccc gcgggccctc
      241 gggcgagcgg aggcggcggc gcacgtggtc ggggaggtcc ccctggccgg tgcgggccca
      301 gatcagcccg acgaccgtgg cggccgccag gctggcgagg aagcgcgcca ggaccacctg
      361 cggccggccg gggaaggcga ccgcggtcgc cacgagcacc acgggattga tcgccggcgc
      421 ggagagcagg aaggccagcg cggcggccgg gggcgcgccg cgggccacca gccgcccggc
      481 gatcggcacc gagccgcact cgcatcccgg caacgccacc ccggcggcgg cggcggcggg
      541 cacggcgagc gccgtccggc gggggaggac gcgtgggagg aagccggcgg ggacgtaggc
      601 ggcgaccgcc gcgctgaccg ccacgccgag caccaggaac ggcatggcct ggacggtgat
      661 cgccacgaag atcgtcgccc agtgggcgac ggcgggggtg tccagggccc gccggagcac
      721 ctgccgcagc agggccgcgg ccagcaccac ggcgcgacca cggccacacc ggcgccgccc
      781 gggcggcgcg cgaccgccag atcaggccgt ctacctgccg gtttcgtgtg gttcatcggg
      841 acatggcagc atatgacccg tcggcggggg cggtcgatcg ggggaggttc gcgatggcgc
      901 gacggtggct gcggggctgg gcggtccagg tgtctctggc ggtggtggcg atcggggtcg
      961 tggtcgccgc gccgctcgcc gcgccggacg gcgcggcgac ggaccgaacg atcatctcgg
     1021 gggcgcgatc gctcgcagac acggtgctgg tgacatacgc cacgccgaag gaccgcacca
     1081 ccgacgtcca cctcctggcg ttcaacgacc tgcacggcaa cctggaggcg gccagcctga
     1141 acctctatgg ccgcttcgcc gggggcgccg cgtacctggc caaggcggtc aaggaccgcc
     1201 aggcgctgtg cgggccctac caggccacca tcctcgccgg ggacaacatc ggcgccagtc
     1261 ccctgcagaa cgggctgttc cacgaggagc cgatcacgat cgccagcaac ctgatgcacg
     1321 tcgacttcgc ctcggtcggc aaccacgagt tcgacaaggg gaaggacgag ctgctgcgga
     1381 tccagaacgg cggctgtcac gccgacgggt gcaccggcgc tccgtacgcc ctggccaacg
     1441 ggtcgagcac cgacgtgtat cccggggcgg atttccagta cctgtcggcc aacgtcgtcg
     1501 tgaacgctac cggggacacc ctcttccctc cgtacgcgat caagaagttc acgtcgaccg
     1561 acggcaagaa gttcagcgtc ggcttcatcg gtgaggtgct cgagtcgacg cccaccatcg
     1621 tcacacccgc cggggtggcc ggcctgacgt tccagcacga ggccgatgcg gcgaacctcg
     1681 ccgtccagga gctcgccgac cgtggcgtga agatccccat cctggtgatc caccagggcg
     1741 gattccagtc ctcgccggcg gccctgaacg gctgcaccgg caacctggcg aacagcgaca
     1801 tcctggagat cgccagccgt ctcgacccgg cgatcaaggt gatcgtctcc gcgcacaccc
     1861 acgccgagta ccgctgcacg atcacgaccg gcggcgtgac tcggctgatc accagcgcct
     1921 cctcgttcgg gcggatcctg tcggacatca ccctcacggt cgacccgagc agcaagaagc
     1981 tgatgcaggc cagcgcgacg aacgtgctgg tggagaacgc gctgaacacc ccgggcccga
     2041 acgtgatccg ccagccggac acctcgaagg aggacccgca ggtccaggcg gtggtgaacc
     2101 agtacgtcgc cgcggccgcc ccgctcgcca accaggtcat cgggcggatc cagggggacc
     2161 tgacgcggac ggcttcccca cagggtgagt cggcgctcgg cgacgtgatc gccgacgccc
     2221 agctggcggc gacggcaccc gccggcctgg gcgacgccgt cctcgcgttc atgaacccgg
     2281 gagggatccg cgccgacctc ctggtcaacg cgatctcctc gggcggtgag gcccccggcg
     2341 aggtcacgta tggcgaggcg ttcgccgtgc agcccttcgg caacagcctg gtgacgaaga
     2401 cgatgacggg cgacatgatc cgccggttgc tgcagcagca gttccagggc tgcggcggca
     2461 ccgcgccggc gaccgggcgc ttcctgcaga tctcggccac gttcagctac gaacagggcg
     2521 catcagcccc ggcctgtgcg gacaagatcg gccggatgtg ggtgaacggc gtcgaggtgg
     2581 ggccgaccga cagcttccgg gtgacgatga acaacttcct ggcgaccggg ggcgacggct
     2641 tcaccgtgtt caacgagggg accaacgccc tcgggggcgc gcaggacatc gacgccttcg
     2701 tcgcctactt cgccgccaac gagccggccg gcatcgcggt cccgccgctc gaccggatcg
     2761 tgccgaagcc ctgaccggcg ggatcggccg ctcgcggcgc cggcctcggg ccggaaccgt
     2821 cccgcgcgcc tatgctgcct ccatgtgccg gaacatcgtg aagctgcggc gccccgaggc
     2881 gacggcgagc gacgacgagg tgcgccaggc ggcgctgcag ttcgttcgca agatcagcgg
     2941 ctaccgggag ccgtcccggc gcaacgccga ggcgttcacg gcggccgtcg acgagatcgc
     3001 cggcgcctcc cggcggctgc tcgacgccct ccccggcccc tccggcgcga ctcgctaggc
     3061 cagcgcgacc agctccagga ggtcgtcgct ccagtggtcg agcgagccct cgggcatgat
     3121 cagcgcgcag tccggggcga gcgcctggac gaactcccgg tcgtgcgtga cgacgatcat
     3181 cgagccactc catgcggcga gcgcccgccc gacggcgctg cgcgagggcg ggtcgaggtt
     3241 gttcgtcggc tcgtcgagca ggatcaggtt gtgccggccg gcgacgagct gggccagcgc
     3301 cagcttcgtc ttctcgccgc ccgagagggt gcccgcgtcc tggaacgcca cctccccggt
     3361 caggccgaac gtccccatca cgccacggag ttgctggtcg ggggccggcg acgcttcgcg
     3421 cacgtggtcc agggcgcgca gacccggccg gattccctcg tgctcctgcg cgtagtagcc
     3481 gagcgcgacg cccgccccca gccggaacgc gccggcgctc ggctcggtcc gcccggcgag
     3541 gatccgcagg aggctggtct tcccggcgcc gttcaggccc atcaccagca gccgctcccc
     3601 gcgcccgagg gtgaacccga catccgtgaa gaccggcggc ccgccatacc ccttggccag
     3661 cccggaagcc tccaggacca gctcgcccga ccggggtggc tcggggaacc gcaccgtcac
     3721 gcgccggtcc cgcctcggcg cgaccaccgc ccggcggccc atccgttcca cgcgccggtc
     3781 gagcgtcttg gcgatgcgcg cccgcttctc ggtctggtgg cgcatcgagt cggcgagcgc
     3841 cgagaggcgg cggatctcgc gctgctgctg tcgcgcggtc ttctcccggc gggcctcgtc
     3901 gagccgccgc gcctccaggt acagcgagta cgtgccgcga tattccacga gcgcggcgtc
     3961 gtcgaggtgc agcacccggg tgatcgagcg gtcgagcagg accaggtcgt ggctgacgag
     4021 cacgagcgcg ccgcggtagg aggacaggaa gcccatcagc caggccttgg cgtcggtgtc
     4081 cagatgattg gtcggctcgt cgagcagcag cagc
//
LOCUS       DASPWO010000411         4906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1080231, whole genome shotgun sequence.
ACCESSION   DASPWO010000411 DASPWO010000000
VERSION     DASPWO010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080231"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VEO00_09420"
     CDS             <1..959
                     /locus_tag="VEO00_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYU58253.1"
                     /translation="RGSAAVLRLGYFPNLTHAPALIGLGSGMLTDALSPTRIEARTFN
                     SGSDASQALLSGALDAAYIGPGPVVTMWVRSGKVAVVSGAVSGGASLIVRTGAGIGAP
                     GDLRGRKVAVPGLGNTQDVALRTWLHDYGLEARDRGGDVTVMSVDNPELAHVFRSGQV
                     DAAWEPEPWPSMLVDQGVAQRLVDESDLWPDGRFVTTTLVVSTPYLDAHPEVVDRLVD
                     GNVAVLRHIHERPIESKVEARRQLLAEGARPLPPQVLAAAWDRLTFDWDPLEGPFEQV
                     AGQMRALGAVEEDAPGLDGLFRLDALNTVLDDEGLPPSEADL"
     gene            1013..1810
                     /locus_tag="VEO00_09425"
     CDS             1013..1810
                     /locus_tag="VEO00_09425"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU58254.1"
                     /translation="MPKLRLRAVRKVFETARGEPVVALDDVSFDVGTGEFVCVVGPSG
                     CGKTTLLHLLAGLDRPSGGEVLLDGTLVPGPAPDRALLFQEPALFPWLSVAGNVELAL
                     RLAGVPREDRRDRTARWLEAVHLGRFAGAQPHELSSGMRQRAALARALACEPAVLLAD
                     EPFASLDAQTREILQNELQGVWQETGVTLVFVTHNVREAVFLADRVLLMSAPPGTLTL
                     EERITAPRPRDYEDVLMAKVTVDIHDHLRKEVAKVVAREIGDRPDRA"
     gene            1770..2552
                     /locus_tag="VEO00_09430"
     CDS             1770..2552
                     /locus_tag="VEO00_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU58255.1"
                     /translation="MSRARSAIGQIGPRAVAIGAVLAVWLAIYASGFFDRSIFPSPLA
                     VGRALADGFRDGSITEALGKSVLRLAFGLSIAIVIGTGIGLAMAGSRLAERGAGSLLL
                     GLLSLPAIAWLPLAILWVGFNDRAVIFVVSLGAFPAVALGTQAAIRQVPPVLERAGRT
                     MGARGWTLYRTVIFPAALPGYLAGLKQGWAFAWLSLIAGELFVGGGLGLGHQLDAARA
                     RLDTPMVLAVMVVIVALGMLVDLVVFGALDRRLRARRGLTVG"
     gene            complement(2573..3325)
                     /locus_tag="VEO00_09435"
     CDS             complement(2573..3325)
                     /locus_tag="VEO00_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58256.1"
                     /translation="MADAGPGSGSADRPAFYALSPGGWRDYWTLLHPPYTLWHLSYVA
                     FGTAAAERFDARRLWATLLAFGLAIGLSAHALDELNGRPLRTRIPSAVLWAIAAAGLA
                     GALGIGVLGVVRATPWLLAYMAVGAFVLVAYNLELLGGRFHSDAWFAAAWGAFPALTA
                     AFAQDEAVRPPHLLVAAACLALSLAQRRLSTPVRTLRRKTRSVHGVQVLEDGTELRLD
                     APALRAPLESALRAISVAMPLLAAGLLWLRFG"
     gene            complement(3318..4037)
                     /locus_tag="VEO00_09440"
     CDS             complement(3318..4037)
                     /locus_tag="VEO00_09440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYU58257.1"
                     /translation="MADSARTRHAGRLFAGIADRYDLLAEVLSFGQNGRWRRFMVSRV
                     PARPGERVLDVATGTAGVARALARATGCRVVGIDPSPEMLAGGAAALRASPAGGRVRL
                     VRGRAERLPFADGAFDHLTFTYLLRYVDDPGGTLAELARVVRPGGTMAALEFAVPANP
                     GARAAWEAYTRFGLPVAGRLASRAWGEAGSFLGWSIRRFYEDHPMEAQEALWREAGIG
                     RVRRRTMSLGGGVVWWGIRGG"
     gene            complement(4045..4863)
                     /locus_tag="VEO00_09445"
     CDS             complement(4045..4863)
                     /locus_tag="VEO00_09445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HYU58258.1"
                     /translation="MGGEAGLYGPGSVSWQVNRETTVLFGGVRAMLLQAAHPLVVAGA
                     RQTGLYRREPWKRLERTLQLSYTITFGTEAEARAVAEHINRVHAEVHGRDEVTGLRYD
                     ALDPDLLLWVHAALIDSALLFERLTVGALDDEGRQRYHREAMAGVELLGLPRERIPPT
                     VPALRAYIDEVIASGILDATTDGARAVATLIRHPPPDTPWRPLLRLLAEWGFGTLPPP
                     LRAGYGVRWNPLREAWLRSSLLGLRAIRRLLPSRVRLIVPARLAERRVASERAA"
BASE COUNT          627 a         1841 c         1816 g          622 t
ORIGIN      
        1 cccggggtag cgccgcggtc ctccgcctcg ggtacttccc gaacctgacc cacgcccccg
       61 ccctgatcgg cctcgggagc ggcatgctga ccgacgcgct ctccccgacc cggatcgagg
      121 cgcggacgtt caattccggg agcgacgcca gccaggcgct gctgtcgggt gccctcgacg
      181 ccgcgtacat cggtccgggg ccggtggtca ccatgtgggt gcggtccggg aaggtggcgg
      241 tcgtctccgg cgcggtctcc gggggcgctt cgctgatcgt tcggaccggc gcgggcatcg
      301 gcgcacccgg cgatctccgc gggcggaagg tcgcggtccc cggactcggc aacacccagg
      361 acgttgccct gcgtacgtgg ctccacgact acgggctgga ggcgcgcgat cgcggcgggg
      421 acgtcaccgt catgtccgtc gacaaccccg aactggcgca cgtgttccgg tcgggacagg
      481 tcgacgcggc ctgggagccc gagccgtggc cgagcatgct ggtggaccag ggcgtcgccc
      541 agcgcctggt cgacgagagc gatctctggc cggacgggcg gttcgtcacc acaacgctcg
      601 tcgtatcgac cccttacctg gacgcgcacc ccgaggtcgt cgatcgcctg gtggacggga
      661 acgtggccgt gttgcgacac atccacgagc ggccgatcga gtcgaaggtc gaggctcgcc
      721 gccagctgct ggcagagggc gcccgcccgc tgccgccgca ggtgctcgcc gcggcctggg
      781 accggctgac cttcgactgg gatccgctgg agggcccgtt cgagcaggtc gccggccaga
      841 tgcgcgcgct gggggcggtg gaggaggacg ccccgggcct cgacggcctc ttccggctcg
      901 acgccctgaa caccgtgctc gatgatgagg ggctcccgcc ttcggaggcc gatctgtgag
      961 ccagcaggga acgcgcgcgc tgccacccgc gacgacgccg gaaccgtcga ccgtgccgaa
     1021 gctccggctg cgcgccgtgc gcaaggtgtt cgagaccgcc cggggtgagc cggtcgtcgc
     1081 tctcgacgac gtctccttcg acgtcggcac gggcgagttc gtctgtgttg tcgggccctc
     1141 gggctgcggc aagacgacgc tcctgcacct gctggccggc ctggaccggc cgtcgggcgg
     1201 agaggtcctg ctggacggca cgctggtccc cgggcccgcc cccgaccgcg cgctgctgtt
     1261 ccaggagccg gccctgttcc cgtggctctc cgtggcgggg aacgtcgagc tggcgctgag
     1321 gctcgccggc gtgccgcgcg aggaccgtcg cgatcggacg gcgcgctggc tggaagccgt
     1381 ccacctcgga cgcttcgccg gcgcccagcc gcacgagctg tcgagtggca tgcggcagcg
     1441 ggcggcgctc gcccgcgccc tggcctgcga gcccgcggtc ctgcttgccg acgagccgtt
     1501 cgcgtcgctc gacgcccaga cgcgcgagat cttgcagaac gagctgcagg gcgtgtggca
     1561 ggagaccggc gtcacgctcg tgttcgtcac tcacaacgtg cgcgaggccg tgttcctcgc
     1621 cgaccgggtg ctcctgatgt cggcgccccc gggcacgctg acgctcgagg agcgcatcac
     1681 ggcgccccga ccgcgggact acgaggacgt gctgatggcg aaggtgaccg tggacatcca
     1741 cgaccatctg cggaaggagg tggcgaaggt tgtcgcgcgc gagatcggcg atcggccaga
     1801 tcgggcctag ggccgtcgcc atcggcgcgg tcctggcggt gtggctggcc atctacgcca
     1861 gtggcttctt cgaccgctcg atcttcccct ccccgctcgc ggtcggccgg gcgctcgccg
     1921 acggcttccg cgacgggagt atcaccgagg ccctcggcaa gagcgtcctt cgcctggcgt
     1981 tcgggctgag catcgccatc gtcatcggaa cgggaatcgg cctcgcgatg gccggctcga
     2041 ggctcgccga gcgcggggcc ggcagcctgc tgctcgggct gctctccctg ccggccatcg
     2101 cctggctgcc gctcgcgatc ctgtgggtcg gcttcaacga ccgagcggtg atcttcgtgg
     2161 tgagcctcgg cgcgttcccc gcggtcgcgc tcgggacgca ggcggccatc cggcaggtcc
     2221 ccccggtcct ggagcgcgcc ggacggacca tgggcgcccg cggctggacg ctctaccgca
     2281 ccgtgatctt cccggcggcg ttgcccggtt acctggcggg cctgaagcag ggctgggcgt
     2341 tcgcctggct ctcgttgatc gctggagagc tcttcgtcgg cggcggcctg ggcctcggtc
     2401 accagctcga cgcggcccgg gcccgtctcg acaccccgat ggtgctcgcg gtgatggtgg
     2461 tgatcgtggc gctcgggatg ctggtggacc tggtggtctt cggggcgctc gaccggcggc
     2521 tgcgcgcccg ccgcggcctg accgttggct gacggcgccg cgggccccgg cttcagccga
     2581 agcgcagcca gagcaggccg gcggcgagga gcggcatggc caccgagatg gcccgcaggg
     2641 ccgactcgag gggcgcgcgc agggccggcg catcgagccg cagctcggtc ccgtcctcga
     2701 gcacctgcac gccgtgcacc gaccgggtct tccggcgcag ggtgcgcacc ggggtggaca
     2761 gacgccgctg cgccagcgac agggccaggc aggccgccgc caccagcagg tgtggggggc
     2821 ggacggcctc gtcctgggcg aacgccgcgg tcagcgccgg gaacgccccc cacgccgccg
     2881 cgaaccaggc gtcggaatgg aaccggccgc cgagcagctc gaggttgtag gcgaccagga
     2941 cgaacgcgcc gaccgccatg tacgccagca gccacggcgt cgcccggacg acccccagca
     3001 cgccgatgcc gagcgccccg gccaggccgg cggcggcgat ggcccacagc acggcggagg
     3061 ggatccgggt gcgcagcggt cgcccgttca gctcgtcgag ggcgtgggcc gacagcccga
     3121 tcgcgagccc gaacgccagc agcgtggccc acagccggcg ggcgtcgaac cgctcggccg
     3181 ccgccgtgcc gaacgccacg tacgacaggt gccacagcgt gtacggcggg tgcagcagcg
     3241 tccagtagtc gcgccacccg ccgggtgaga gcgcgtagaa cgccgggcgg tcggcggatc
     3301 cgcttcccgg gccggcgtca gccaccgcgg atcccccacc agaccacgcc gccccccagg
     3361 ctcatcgtcc gccgccgcac gcgcccgatc ccggcctcgc gccacagggc ctcctgcgcc
     3421 tccatcgggt ggtcctcgta gaaccggcgg atgctccagc cgaggaagga ccccgcctcc
     3481 ccccaggccc gcgaggccag gcgtccggcg accggcaggc cgaaccgcgt gtacgcttcc
     3541 cacgccgcgc gggcgccggg attcgcgggg acggcgaact ccagcgcggc catcgtcccg
     3601 cccggccgga ccacccgcgc cagctcggcg agcgtgccgc ccgggtcgtc cacgtaccgc
     3661 agcaggtacg tgaacgtcag gtggtcgaac gcgccgtcgg cgaacgggag gcgctccgcc
     3721 cggccgcgca ccagccggac gcgcccgccc gccggcgacg cgcgcagcgc cgccgcgccg
     3781 ccggcgagca tctcgggtga ggggtcgatc ccgaccacgc ggcaccccgt cgctcgggcg
     3841 agcgcccgcg ccacgccggc ggttccggtg gcgacgtcga ggacccgctc ccccggccgg
     3901 gccggcaccc gcgagaccat gaaccgccgc cagcgaccgt tctgtccgaa cgacagcacc
     3961 tcggccagca ggtcgtaacg gtcggcgatg ccggcgaaca ggcgaccggc gtgccgggtc
     4021 ctggccgagt ccgccacggt cccctcaggc ggcgcgttcc gacgcgaccc gccgctcggc
     4081 gagccgggcc gggacgatca gccggacgcg cgaggggagg agccgccgaa tggcgcgcag
     4141 gccgaggagc gaggagcgca gccaggcctc gcgcagcggg ttccagcgca cgccgtagcc
     4201 ggcgcggagc ggcggcggca gcgtcccgaa cccccactcg gccagtaagc gcaggagcgg
     4261 ccgccacggc gtgtccggcg gcgggtgccg gatcagggtc gccaccgcgc gagctccgtc
     4321 ggtggtcgcg tcgaggatgc cgctcgcgat cacctcgtcg atgtatgcgc gcagcgccgg
     4381 aaccgtcgga gggatccgct cgcgcgggag gccgagcagc tccacgccgg ccatcgcctc
     4441 gcggtggtac cgctgccggc cctcgtcgtc gagggcgccg acggtgagcc gctcgaacag
     4501 gagcgcggag tcgatcagcg cggcgtggac ccacagcagc aggtcggggt ccagcgcgtc
     4561 gtaccgcagc ccggtgacct cgtccctgcc gtgaacctcg gcgtggacgc gattgatgtg
     4621 ctcggcgacg gcccgcgcct cggcctcggt gccgaacgtg atcgtgtacg agagctgcag
     4681 cgtccgctcc aggcgcttcc acggctcgcg ccggtacaga ccggtctgcc gcgccccggc
     4741 caccacgagc gggtgcgccg cctgcagcag catcgcccgg acgccgccga acagcacggt
     4801 ggtctcgcgg ttcacctgcc aggagaccga ccccgggccg tagagccccg cctcgccgcc
     4861 catcgaggga aggctaccgc taccatcgcc cgccatggtc gccccg
//
LOCUS       DASPWO010000412         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_571220, whole genome shotgun sequence.
ACCESSION   DASPWO010000412 DASPWO010000000
VERSION     DASPWO010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571220"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VEO00_09450"
     CDS             <1..870
                     /locus_tag="VEO00_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HYU58259.1"
                     /translation="ANNGMWTLIDNHPNWLSAFRYPDWQYTAPYNSHDKDYPATLEGS
                     QEAETDFWTDDLRQQFVDEMWRYLAALVGDEPGVAGYAPMSEPEPGNLPYVHTTTATI
                     LDWQAKQSLSIRAVDPDAIIFFTTRNGFGPGLPTADLSDYTAMGNVAFDLHDYFGARL
                     GSGLGPTDPASPNYMEGRQAIYAASDDGSGNEHPYAYTGTVDSQIRFLKSHMKYLKPL
                     GIPMYVGEFTTDGSVDPGVFLFHGTVTSALNQLSLSWTVSNYWGPLGVVSAPGGPLNP
                     WGCILVEAMGGTC"
     gene            936..>2103
                     /locus_tag="VEO00_09455"
     CDS             936..>2103
                     /locus_tag="VEO00_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYU58260.1"
                     /translation="MTPLRRSRALAVFLILAAAGTLLVQTSGAIRAEAVQNADWDTFG
                     GDAYRTGYNPNETVIGTGNVGNLHLLWSFDLGAVTISQPAYAAGVNVGGTAKDLVLAG
                     SEHGMLFALDATNGQVVWSRYLGDQQTGCGDMPDGRFGVSGTPAIDRSANRVYEAGGD
                     GAVYALDLSTGATIPGWPIAVTPDPTHEHVYGALTLYLNRLYATVASYCDFTPYHGKV
                     VGIDTSMPARTAVWYPVGNGGVSGGGIWGPGGVSLDPGNGHVFAATGNALTFPENYRY
                     AETVDELGPRLRVLGSNKPSLTGGDVDFGATPLLYQAPGCPPQAVAKNKSGVLLLYDR
                     GQLNAGYVQRLQIASVGDWEFNGIPAYDPATNMVYIGNSSDAGIYTHGMVTLAVQ"
BASE COUNT          350 a          729 c          702 g          322 t
ORIGIN      
        1 gcgaacaacg ggatgtggac actgatcgac aaccacccga actggctgtc ggccttccgg
       61 tacccggact ggcagtacac cgcgccctac aactcgcatg acaaggacta tcccgccacg
      121 ctcgaggggt cacaggaagc ggagacggat ttctggaccg acgacctccg ccagcagttc
      181 gtggacgaga tgtggcggta cctggccgcg ttggtggggg acgagccggg ggtggccggc
      241 tacgctccga tgagcgagcc ggagcccgga aacctcccct acgtccacac cacgaccgcg
      301 acgatcctgg attggcaggc gaagcagtca ctcagcatcc gggcggtcga tcccgacgcc
      361 atcatcttct tcaccaccag gaacgggttc ggccccggcc ttccgacggc cgacctgtcc
      421 gactacacgg cgatgggcaa cgtcgcgttc gacctgcacg actacttcgg cgccaggctc
      481 ggctccgggc tcggccccac cgaccccgcg agtcccaact acatggaggg gcggcaggcc
      541 atctatgcag cgtcggacga cgggagcggg aacgagcacc cctacgccta cacgggcacc
      601 gtggactccc agatccgatt cctgaagtcc cacatgaagt acctcaagcc gttgggcatc
      661 ccgatgtacg tcggcgagtt cacgaccgac ggctccgtcg atcccggggt gttcctcttc
      721 cacggcaccg tgaccagcgc cctgaaccag ctctccctgt cgtggaccgt gagcaactat
      781 tggggccccc tcggcgtcgt ctccgcgccg ggcgggccgc tgaacccctg gggctgcatc
      841 ctggtcgagg ccatgggcgg tacctgctga gcttcggatc ccgttcgagt ctcaccgctc
      901 gcaacgtgtc gtggaggcgt accatcctgc ccgccgtgac gcccctccgc cgatctcgcg
      961 cgctcgcggt cttcctcatc ctcgcggcgg ccggtacgct cctcgtccag acctccggcg
     1021 ccatccgcgc ggaggccgtt cagaacgccg actgggacac gttcggtggc gacgcctatc
     1081 gcacggggta caacccgaac gagaccgtca tcgggacggg aaacgtcggg aacctgcacc
     1141 tgctgtggtc gttcgacctc ggggccgtca cgatcagcca gccggcctat gcggccggcg
     1201 tgaacgtcgg agggacggcc aaggacctgg tcctggccgg atccgagcac ggcatgctct
     1261 tcgctctcga cgcgacgaac gggcaggtcg tgtggtcgcg atacctcggg gaccagcaaa
     1321 cggggtgcgg cgacatgccg gacggccggt tcggggtgag cgggacgccg gccatcgacc
     1381 ggtcggcgaa tcgggtctac gaggccggcg gtgacggagc ggtgtacgcc ctcgatctct
     1441 cgacgggagc gaccatccct ggctggccca tcgcggtcac gccggatccg acccatgagc
     1501 acgtctacgg cgcgctgacc ctctacctga accgcctcta tgccacggtg gcaagctact
     1561 gcgacttcac gccctatcac gggaaggtcg tggggatcga cacctcgatg ccggccagga
     1621 cggcggtgtg gtacccggtc gggaatggcg gcgtcagcgg cggcgggatc tgggggccgg
     1681 gcggggtatc cctggacccg ggcaacggcc acgtgttcgc cgccaccggg aacgcgctga
     1741 cgttcccgga gaattatagg tacgccgaga ccgtcgacga gctcggccct cggctgcggg
     1801 tcctggggtc gaacaagccg agcctgaccg ggggcgacgt cgacttcggc gcgactccgt
     1861 tgctctacca ggcgccgggg tgcccgcctc aggcggtcgc gaagaacaag tcgggggtgc
     1921 tgctgctcta cgaccgggga cagctcaacg ccggatacgt gcagcggctg cagatcgcca
     1981 gcgtcggcga ctgggagttc aacgggatcc ccgcctacga ccccgcgacg aacatggtct
     2041 acatcggcaa ctcctcggac gccgggatct acacgcacgg gatggtgacc ctggccgtcc
     2101 agg
//
LOCUS       DASPWO010000413         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_827469, whole genome shotgun sequence.
ACCESSION   DASPWO010000413 DASPWO010000000
VERSION     DASPWO010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827469"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..765)
                     /locus_tag="VEO00_09460"
     CDS             complement(55..765)
                     /locus_tag="VEO00_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58261.1"
                     /translation="MPRTRPRGRHAPRSRPSGRGLPLAVAALSAGAGAIHAAVVREHA
                     AEYWLFGAFFVLSAVLQLAWAWLILARPSRGLYALGAAGQALVVLIWVVSRTAGLPVG
                     PDAGLPEAAGAADTLSTVLEVLAVFGALAVLRAHAERPTPSVLSGAGAGILAAGLAMA
                     ALFAYLLGPGGSGEDTAAGPAAAAGHGVHALVILVAGGLFLVFAILDWVRGGPRGFSW
                     RLKDPQDSSTGTPSSPSR"
     gene            828..1268
                     /locus_tag="VEO00_09465"
     CDS             828..1268
                     /locus_tag="VEO00_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HYU58262.1"
                     /translation="MDEGEMRRLVAEGRVARLGTIDDRGRAHLVPFVYVLDGDTIYTG
                     VDRKPKRSTRLKRLANLRADPRATLLVDHYEEDWTRAWWVRVRGRARILEDGPERDHA
                     ARLLAEKYPQYEGDPPDDIVIAIGADDWYGWAFTEDGERPPAPG"
     gene            complement(1276..1689)
                     /locus_tag="VEO00_09470"
     CDS             complement(1276..1689)
                     /locus_tag="VEO00_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYU58263.1"
                     /translation="MRPFALAIAVPVLLLFAACGGGGSTTASPTPGGATGSLACTDLT
                     TTDPGAIAMHDFVFVPDCVVALNTQALTLSNDGTALHNFSLAHTSIDVDVQPGESRDL
                     QPPGAALAPGTYAFFCKYHRSQGMQGIIRVDSGPA"
     gene            complement(1710..>2571)
                     /locus_tag="VEO00_09475"
     CDS             complement(1710..>2571)
                     /locus_tag="VEO00_09475"
                     /inference="COORDINATES: protein motif:HMM:NF021926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HYU58264.1"
                     /translation="FHVRFEGMTGGGTFSGWPGRDPGTYLLLCDPFTFPAEPFLRELN
                     DQAPGTVVIGGNASGGAGPGGTRLFIDRRVVEDGAVGCRAPGAVRALVSQGCRPVGRP
                     FTVTRSDGNVIHELAGTPAVQRLQETWDAAAPPDRELIANGLLIGRVVNEYRSEFGPG
                     DFLVRGVIGADRESGAIAVADHVTVGETVQFHVRDAATADEELRTLLERRLGPDAGRV
                     AGALLFTCNGRGTHLFPEPDHDARMVDTLLAVPTAGFFAAGELGPVGDRTFVHGFTAS
                     LAVFLDGAER"
BASE COUNT          373 a          954 c          963 g          281 t
ORIGIN      
        1 ggttcgcacc ggcggcgggc tcgggggcta cgggggcggc ctgtggcgga agcgctacct
       61 gctggggctg gagggcgtcc cggtcgagga gtcctgaggg tccttcaacc tccacgagaa
      121 cccgcggggc ccgccgcgga cccagtccag gatggcgaag acgaggaaga gccctccggc
      181 gaccaggatc accagcgcgt ggacgccgtg ccccgccgcg gccgccggtc ccgccgcggt
      241 gtcctcgcca gacccgcccg ggccgagcag gtaggcgaag agcgcggcca tcgccaggcc
      301 ggcggcgagg atcccggcgc ccgcccccga gagcaccgat ggggtcggcc gctccgcgtg
      361 ggcacgcagg accgccagcg ccccgaagac ggccaggacc tccagcaccg tggacagggt
      421 gtcggcggcg ccggccgcct cggggaggcc ggcgtccggc ccgaccggca acccggccgt
      481 ccgcgacacg acccagatca ggacgacgag cgcctgtccc gcagcgccca gcgcgtagag
      541 cccgcgcgag gggcgggcca ggatcagcca ggcccaggcc agctggagca cggcggagag
      601 cacgaagaag gcgccgaaca gccagtactc ggccgcgtgc tcccggacca cggcggcgtg
      661 gatcgccccg gcgcccgcgg agagcgcggc caccgccagc ggcagcccgc gcccggacgg
      721 gcgggatcgc ggcgcgtgcc ggccgcgcgg tcgcgttcga ggcatggcgt ccacgatcgc
      781 ccaccatacc ccccgtaccg gacgccggat ggcggtacgg ttcggccgtg gacgagggcg
      841 agatgcgccg gctggtggcc gagggacgcg tggcgcggct cgggacgatc gacgaccgcg
      901 ggcgtgccca cctcgttccg ttcgtctacg tcctggacgg cgacacgatc tacacgggcg
      961 tggaccgcaa gccgaagcgc tccacgcgcc tgaagcgcct ggcgaacctg cgcgccgacc
     1021 cccgcgccac gctcctggtc gaccactacg aggaggactg gacgcgcgcc tggtgggtgc
     1081 gcgtccgcgg ccgggcgcgc atcctggagg acgggccgga gcgcgaccac gcggcgcgcc
     1141 tgctggcgga gaagtaccca cagtacgagg gcgacccgcc cgacgacatc gtcatcgcca
     1201 tcggggccga cgactggtac ggctgggcct tcaccgagga cggggagcgc ccgccggcgc
     1261 cgggctgagg ccgggtcagg ccggcccgga gtcgacccgg atgatcccct gcatcccctg
     1321 gctgcggtgg tacttgcaga agaacgcgta ggtccccggc gccagggccg cgccgggggg
     1381 ctgcaggtcc cgcgactccc ccggctgcac gtcgacgtcg atggacgtgt gcgccagcga
     1441 gaagttgtgc aacgccgttc cgtcgttcga cagcgtcagc gcctgcgtgt tcagggcgac
     1501 gacgcagtcg gggacgaaca cgaagtcgtg catggcgatc gcgcccggat ccgtcgtcgt
     1561 caggtcggtg caggcgagcg atccggttgc ccccccgggc gtgggtgacg ccgtggtcga
     1621 tcctccgcca ccgcacgcgg cgaagagcag caggaccgga acggcgatcg cgagggcgaa
     1681 cggtcgcatc gaacctcctt cgagatcgtt cagcgttccg cgccgtcgag gaacacggcc
     1741 aggctggcgg tgaagccgtg gacgaacgtg cggtcgccga ccgggccgag ctcgccggcg
     1801 gcgaagaacc cggccgtcgg gaccgcgagc agcgtatcga ccatccgggc gtcgtggtcg
     1861 ggctcgggga acaggtgcgt cccgcggccg ttgcaggtga acagcagcgc gccggccacg
     1921 cgcccggcgt ccggcccgag gcgcctctcc agcagggtgc gcagctcctc atcggcggtg
     1981 gcggcgtcgc gcacgtggaa ctgcacggtc tcgcccacgg tgacgtggtc ggccacggcg
     2041 atcgcgccgc tctcgcgatc ggcgccgatc acgccgcgga ccaggaagtc gcccggtccg
     2101 aactccgagc ggtactcgtt cacgacgcgc ccgatcagca ggccgttcgc gatcagctcg
     2161 cggtcgggcg gcgcggccgc gtcccacgtc tcctgcagcc gctgcacggc cggcgtaccg
     2221 gccagctcgt ggatgacgtt gccgtctgac cgggtcaccg tgaaaggccg gcccaccggg
     2281 cggcagccct gcgagacaag cgcccgcacc gcacccggcg cgcggcaccc gacggcgccg
     2341 tcctcgacca cccgccgatc gatgaagagc cgggtcccac cagggccggc gccgccgctg
     2401 gcgttcccgc cgatcacgac ggtccccggc gcctggtcgt tcagctcgcg caagaacggc
     2461 tcggccggga acgtgaacgg gtcgcacagc agcaggtagg tcccggggtc ccgacccggc
     2521 cacccgctga acgtgccgcc gccggtcatc ccctcgaacc gcacgtggaa c
//
LOCUS       DASPWO010000414         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_401211, whole genome shotgun sequence.
ACCESSION   DASPWO010000414 DASPWO010000000
VERSION     DASPWO010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401211"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VEO00_09480"
     CDS             <1..345
                     /locus_tag="VEO00_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYU58265.1"
                     /translation="YRERLAGVPGILMADDPPDGTTNFQSFWVLLADGFPVGRDALLE
                     RLMEHGISARRGIMAAHLEPAYADLPSVALPVTERLTGRSLILPLFHAMTQEEQDLVI
                     AVVWDASGVAGR"
     gene            342..1076
                     /locus_tag="VEO00_09485"
     CDS             342..1076
                     /locus_tag="VEO00_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HYU58266.1"
                     /translation="MSGRPLLIVGAGGFGREAAEAVRAVNAERPRTSPGSGWELLGFL
                     DDAPALQGREVDGLPVLGPLSSLERFADAMLVVSVGNPENYFARRRIVRRLGLPPSRY
                     ATIVHPAAVLPPSAEVGPGSVLLAGVVATTSIRIGAHVVAMPGVVLTHDDAVADFVTL
                     GAGVRLAGGVRVEEGAYVGAGVLVREDRTIGSWALVGMGAVVTRDVPAGEVWAGVPAR
                     FLRRVEGAPDPPPSEPAHPVSAVHER"
     gene            1117..2208
                     /locus_tag="VEO00_09490"
     CDS             1117..2208
                     /locus_tag="VEO00_09490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU58267.1"
                     /translation="MSIIIPCYNYGRYLRECVASVLSQEGVRVSVLIIDDASTDDSFQ
                     VAAELAAGDRRVCVERHRTNKGHIATYNEGLARVSGRYTVLISADDLLAPGSLERATA
                     LMEAHPSVGFVYGGNVRFRSGEQLPGPRVFPEPPSSQVQAGREWLTALCRAGRNIIAS
                     PEVVVRTDLQQRLGGYREELPHSGDLEMWMRFAAHGDVGICRNVVHAYVRDHDTNMQR
                     TRFASQVSRLVQRRDAFDYAFGHYREKIDPTGRLRRAADRSLRADAMWWVLHCVYHRQ
                     LDAQLAGLLKLVLGVGGDPGGSPGKVRRGARIAVRLLEMLPLLVRGARRARQDRAEPG
                     QPASFVSTIAVRRPILVSPGIADRAEHRA"
BASE COUNT          325 a          855 c          872 g          363 t
ORIGIN      
        1 taccgcgagc gcctggccgg cgtgcccggg atcctcatgg cggacgaccc gcccgacggg
       61 acgaccaact tccagtcctt ctgggtcctg ctcgccgacg gcttcccggt cgggcgggac
      121 gcgctcctcg agcggctgat ggagcacggc atctcggccc gccgcggcat catggcagcg
      181 cacctggagc ccgcctacgc cgacctccca tcggtcgcgc tgccggtcac agagcgcctg
      241 acgggcaggt ccctgatcct gccgctgttc cacgcgatga cccaggagga gcaggacctg
      301 gtgatcgccg tggtctggga cgcgagcggg gtggccggcc ggtgagcggg cgcccgctgc
      361 tcatcgtcgg cgccggcggg ttcggccgcg aggccgccga ggcggtgcgt gcggtcaacg
      421 cggagcggcc cagaaccagt cccgggtccg ggtgggagct gctcgggttc ctggacgacg
      481 ccccggcgct ccagggccgg gaggtggacg gcctccctgt cctcggcccg ttgtccagtc
      541 tggagcggtt cgccgacgcc atgctcgtgg tctcggtcgg gaacccggag aactatttcg
      601 cccgaaggcg gatcgtgcgg cgcctcggcc ttccgccctc ccgctatgcg accatcgtcc
      661 accccgccgc cgtgctgcct ccgagcgccg aggtcggtcc aggcagcgtg ctcctcgccg
      721 gggtggtggc gaccacctcg atccggatcg gcgcgcacgt cgtcgcgatg cccggtgtgg
      781 tgctcaccca cgacgacgcg gtcgccgact tcgtgaccct gggggcgggt gtgcggctgg
      841 cgggcggcgt gcgggtcgag gagggcgcct atgtcggagc gggcgtcctg gtccgggagg
      901 atcggaccat cgggtcgtgg gcgcttgtcg gcatgggcgc cgtcgtgacc agggacgtcc
      961 ccgctgggga ggtctgggcc ggcgtgccag cccggttcct gcgccgtgtc gagggagccc
     1021 ctgatccgcc cccgtctgaa ccggcccatc ccgtctccgc cgtgcacgag cgatagacca
     1081 ggacaccgca tgccctcagg aagcgccacg ccgacggtga gcatcatcat cccctgctac
     1141 aactacggca ggtacctcag ggagtgcgtt gcgagcgtgc tctcccagga gggcgtgcgg
     1201 gtgagcgtgc tcatcatcga cgacgcctcg acggacgact cgttccaggt cgccgccgag
     1261 ctcgccgccg gcgaccggcg ggtatgtgtc gagcgccacc ggaccaacaa ggggcacatc
     1321 gccacctaca acgagggcct ggcgcgggtc agcggcaggt acacggtcct gatctcggcc
     1381 gacgatctgc tcgccccggg ctcgctcgag cgggcgaccg cactgatgga ggcgcatccg
     1441 agcgtcggct tcgtctacgg cggcaacgtc cggttccgct cgggcgagca gcttcccggt
     1501 ccaagggtct tcccggagcc gcccagctcg caggtccagg ccggccggga gtggctcacc
     1561 gccctgtgcc gggccggacg caacatcatc gcgtcgcccg aggtggtcgt ccggaccgac
     1621 ctccagcagc ggctcggagg gtaccgggag gagctgccgc actccgggga cctcgagatg
     1681 tggatgcgct tcgccgcgca cggcgacgtc gggatctgcc gcaacgtggt ccacgcctac
     1741 gttcgggacc acgacaccaa catgcaacgg acgcgcttcg cctcccaggt cagccggctg
     1801 gtccagcgac gggacgcctt cgactacgcc ttcggtcact acagggagaa gatcgacccg
     1861 accggccgct tgcggcgggc cgccgatcgc agcctgcggg cggacgccat gtggtgggtc
     1921 ctgcactgcg tgtaccaccg gcagctcgac gcgcagttgg ccgggctcct gaagctggtg
     1981 ctgggcgtcg gcggcgatcc cggcgggtcc cccggcaagg tccggcgcgg cgcgcggatc
     2041 gccgtccgtc tgctggagat gcttccgctc ctggtcaggg gggcgcgcag ggcccggcag
     2101 gatcgcgcgg agcccggcca gccggcgagc ttcgtctcca cgatcgcggt tcgacggccc
     2161 atcctggtgt cgccaggcat cgccgaccgt gcggagcacc gggcatagcg gacggatgac
     2221 gtccgcgctg catgaagtac tgcacttgcc ggtagccgga ggagcaggtc tgcccatacg
     2281 gtgatcacct gtcgatccag gtggaaagga tcacgacctg ttctcgtacc agctacctca
     2341 cggcggacag ggcatgctcg tagaaggcca gcatcccctc ggctgcccgg gtgatcgtga
     2401 agcgcgcctc gaact
//
LOCUS       DASPWO010000415         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_827857, whole genome shotgun sequence.
ACCESSION   DASPWO010000415 DASPWO010000000
VERSION     DASPWO010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827857"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VEO00_09495"
     CDS             complement(<1..449)
                     /locus_tag="VEO00_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017957334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HYU58268.1"
                     /translation="MEVIERGRALRSRLDAERSAGTTVGFVPTMGAFHEGHLGLLKRA
                     VAERDLVVVSIFVNPLQFGPGEDFEAYPRQRERDLALAEEAGAALAYVPDLVDVYPSW
                     PLEVSVDPGPLGERLEGAARPGHFRGVATVVAKLFNRVGPSTAYFGE"
     gene            complement(440..1351)
                     /locus_tag="VEO00_09500"
     CDS             complement(440..1351)
                     /locus_tag="VEO00_09500"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HYU58269.1"
                     /translation="MEDQLLSQPLQLAVIGAGRVGTALAVLLSRAGHRIVAVSGRHGT
                     EDRAARYLPGVPVLQPAQAAALANCVLLSVPDDLVRPHCSEISTKLHRGVGVGHVSGS
                     LGLDVLAPAAHWGAVVFSLHPLQTVPDVEGGVSRIPGSAFAVTAANETGYAIGERLAR
                     EAGGEPFRIRNEAKPLYHAAAVFASNYLTTVEAVAEKLFRIAGLQDPRSAMSPLAAAT
                     LMNALRRGPGDALTGPAVRGDVRTIERNLTALRQLAPETIPAYIALARLALDLAEDAG
                     RLDGHARRRVEDLLSQKKWEVQQRRWR"
     gene            complement(1496..>2126)
                     /locus_tag="VEO00_09505"
     CDS             complement(1496..>2126)
                     /locus_tag="VEO00_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58270.1"
                     /translation="LDVVRVSPDGRRHPFAHVPRSLGEQPDLVAAAGRVYLAYTSRVL
                     RVLPSGSVRTVLSGWQVLGESERGMLALLPSRPSGEIGQPLRLHWWFPSWEREPAPVP
                     SAFEAFQVLGWSSDSSAVAALGELDGRPGLWVVPLDRNATPQMVLEGDPEAGFSVGPV
                     AFAADDRFAFFATEDGLFGFDRSLGKLSKLQLPPDLERRVPNGPMVWVP"
BASE COUNT          312 a          798 c          762 g          254 t
ORIGIN      
        1 ttctcgccga agtacgcggt tgacggcccc acccgattga acagcttggc cacgaccgtc
       61 gccacgccgc ggaagtgccc cggccgggcg gccccctcca gccgttcccc gagcgggccc
      121 ggatcgacgg acacctcgag cggccaggaa ggatagacgt cgaccaggtc gggcacgtac
      181 gccagggccg ccccggcctc ctccgccagc gccaggtcgc gctcccgctg gcgcgggtag
      241 gcctcgaagt cctcccccgg cccgaactgg agcgggttga cgaagatcga cacgacgacg
      301 aggtcccgct cggcgaccgc ccgcttcagc agcccgaggt gtccctcgtg gaaggcgccc
      361 atcgtcggga cgaacccgac ggtcgttccg gcggaccgct cggcatccag gcgcgaccgc
      421 agcgcgcggc cgcgctcgat cacctccacc gccgttgctg cacctcccac ttcttctggg
      481 acaggaggtc ctccacgcgc cgccgggcgt gcccgtcgag gcgcccggca tcctcggcca
      541 ggtcaagcgc cagccgcgcc agggcgatgt acgccgggat cgtctccggg gccagctgcc
      601 ggagggcggt caggttccgc tcgatcgtgc gcacgtcgcc ccgcaccgcc ggcccggtca
      661 gcgcgtcgcc ggggccgcgg cgcagcgcgt tcatcagcgt cgccgccgcg agcggagaca
      721 tcgccgagcg cggatcctgc aggccggcga tgcggaagag cttctccgcc acggcctcca
      781 ccgtggtcag gtaattcgag gcgaacaccg ccgccgcgtg gtacaggggc ttcgcctcgt
      841 tgcggatccg gaacggctcg ccaccggcct cccgggccag gcgctccccg atcgcgtagc
      901 cggtctcgtt cgcggcggtc accgcgaacg cgctgccggg gatgcggctg accccgccct
      961 ccacgtcggg cacggtctgc agcgggtgca gcgagaacac gaccgcgccc cagtgcgccg
     1021 ccggggcgag gacgtcaaga ccgagcgagc cgctgacgtg gccgacgccg acgccgcggt
     1081 ggagcttggt cgagatctcg gaacagtgcg ggcgcaccag atcgtcgggg acgctgagca
     1141 gcacacagtt ggccagggcg gccgcctgcg ccggctggag gacggggacg ccgggaaggt
     1201 accgggcggc gcggtcctcg gtgccgtgtc ggccggagac ggccacgatg cgatgcccgg
     1261 cgcgcgacag cagcacggcg agcgccgtcc cgaccctccc cgcgccgatc acggcgagct
     1321 ggagcggctg gctgaggagc tggtcttcga tggccggaaa acccctcccc ttcctctgtc
     1381 tttggggtcc gctcgccgga ggtggtcagg tcgatcccgt cacggggcga gctaccccca
     1441 aacccccggc cggacggcgc ggagtatacg gtgagcgcgt ggccggggtt tcgtcctacg
     1501 ggacccagac catcgggcca ttcgggacgc ggcgctccag gtcgggtggg agctgcagct
     1561 tcgacagctt gccgagagac cggtcgaacc cgaacaggcc gtcctccgtg gcgaagaacg
     1621 cgaaacggtc gtccgcggcg aacgccaccg ggcccaccga gaaaccggct tccgggtcgc
     1681 cctcgagcac catctgggga gtggcgttcc ggtcgagggg caccacccac aggcccggcc
     1741 ggccgtcgag ctccccgagc gcggccaccg cggacgaatc ggagctccac cccaggacct
     1801 ggaaggcttc gaacgccgac gggacggggg ctggctcccg ttcccaggag gggaaccacc
     1861 agtgcagccg cagcggctgc ccgatctctc cggagggccg ggacgggagg agggcgagca
     1921 tgcctcgctc gctctcgccg agcacctgcc acccgctcag cacggttcga accgacccgg
     1981 acggcagcac ccgcagcacg cggctggtgt atgccaggta gacgcgcccg gccgccgcga
     2041 ccaggtccgg ctgctcgccg agcgagcgcg ggacgtgcgc gaacggatgc cgccgtccat
     2101 ccggcgagac ccgcacgacg tccagg
//
LOCUS       DASPWO010000416         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_401245, whole genome shotgun sequence.
ACCESSION   DASPWO010000416 DASPWO010000000
VERSION     DASPWO010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401245"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VEO00_09510"
     CDS             complement(<1..435)
                     /locus_tag="VEO00_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYU58271.1"
                     /translation="MPADLRLAGGRVWTRGRLAPVDLLVAGDRISAVVGPETETPGVD
                     RVVDCTGKVILPGMIDVHVHTREPGFTAKEDIATATAQAAAGGVTTIFGMPNLDPPTT
                     TVEALEEVFGLYERKAIVDWNHNPAPTQLDQIGKMAEMGVRAF"
     gene            complement(435..1379)
                     /locus_tag="VEO00_09515"
     CDS             complement(435..1379)
                     /locus_tag="VEO00_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYU58272.1"
                     /translation="MKVEDVAFYSEGERVAGLWRTPDEAAGPFPAIVHGPGWMGLRDA
                     KLYVRYHEALTQAGFGVLVIDYRGFGDSGGDPERMSVEGQVADLVNAVTYLTTREDVR
                     REAIGAFGSGGTGGGNAILLAAADPRVRAVVSQLPVADGQDWLHRMRSEHEWVDFLKA
                     LEEDRRDRVLRGEGRLVNPREEIMIPTPERRTTTVKSDVDARIPTKVSLRLADEILRY
                     RPIDAAAGLATPLLVIGIEDDAVTPTDHAVHLYEAAAGPKELVMQRNTTHYAAYDQYW
                     ESVTPRIVQWFTTYVRSGDLLVRTRLPEGERTDWLGPA"
     gene            complement(1506..>2115)
                     /locus_tag="VEO00_09520"
     CDS             complement(1506..>2115)
                     /locus_tag="VEO00_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYU58273.1"
                     /translation="RVDVLVSNAGYGVGGFVEDTTMEEFREQFETNFFGGVRVVKAVL
                     PGMRERRSGRIILISSIGVFNSTPMLSAYNATKAALEAFGEALRLETVHDGVFVTLIE
                     PGTYATDIFFDNAKRAEGMVDPSSPHYEEGQRMERFAMKQVERRRKADPREVARKVVQ
                     VATSRRPKLRYLVGSDARVLKPVRAVTPTRVTEAAVRKIMRG"
BASE COUNT          278 a          782 c          725 g          330 t
ORIGIN      
        1 gaacgcccgc acgcccatct ccgccatctt cccgatctgg tccagctggg tgggcgccgg
       61 gttgtggttc cagtcgacga tggccttccg ctcgtagagg ccgaacacct cctccagcgc
      121 ctccacggtg gtcgtgggcg ggtccaggtt cggcatgccg aagatcgtgg tgacgccgcc
      181 ggccgccgcc tgggccgtcg cggtggcgat gtcctccttc gcggtgaagc cgggctcgcg
      241 ggtgtggacg tggacgtcga tcatgcccgg caggatcacc ttccccgtgc agtcgacgac
      301 gcgatccacg ccgggcgtct cggtctcggg cccgaccacc gccgagatgc ggtcgcccgc
      361 gacgaggagg tccaccgggg ccaggcggcc gcgcgtccac acccgccctc cggccagccg
      421 aaggtcggcc ggcatcaggc cgggccgagc cagtccgtcc gctcgccctc ggggaggcga
      481 gtgcggacca ggaggtcgcc gctccggacg tacgtggtga accactgcac gatgcgcggc
      541 gtcaccgact cccagtactg gtcgtacgcg gcgtagtgcg tcgtgttccg ctgcatgacg
      601 agctccttcg gccccgccgc ggcctcgtac aggtgcacgg cgtggtcggt cggcgtcacc
      661 gcgtcgtcct cgatgccgat gaccagcagc ggcgtggcca ggcccgccgc cgcgtcgatg
      721 gggcggtacc gcaggatctc gtccgccagg cgcagcgaca ccttcgtggg gatgcgggcg
      781 tcgacgtcgc tcttcaccgt ggtggtgcgg cgctccggcg tggggatcat gatctcctcg
      841 cgggggttca ccaggcgccc ctcgccccgc aggacccggt cccggcggtc ctcctccagg
      901 gccttcagga agtccaccca ctcgtgctcg gagcgcatgc ggtggagcca gtcctggccg
      961 tccgccaccg gcagctggct gaccacggcc cgtacgcggg ggtcggcggc ggccagcagg
     1021 atggcgttcc cgccccccgt ccccccgctg ccgaacgcgc cgatggcctc gcgccgcacg
     1081 tcctcccggg tggtcaggta cgtgacggcg ttgaccaggt cggcaacctg tccctccacg
     1141 gacatccgct ccgggtcgcc gcccgagtcg ccgaaccccc ggtagtcgat caccagcacg
     1201 ccgaaccctg cctgcgtgag ggcctcgtgg tagcgcacgt acagcttggc gtcccgaagg
     1261 cccatccacc ccgggccatg gacgatggcg ggaaacggtc cggcggcctc gtcgggagtc
     1321 cgccacaggc cagcgacgcg ctctccctcg ctgtagaagg ccacgtcctc gaccttcacc
     1381 ggcccctccc cgggagcgaa cgtccgctgt cgtcggttgg atattggata cgagaggcaa
     1441 tatagcctcg gccgggggtc ccgtccaggg gccgcgcggg atgtcgacag gcgtgcccgg
     1501 cggcgtcagc ctcgcatgat cttgcgcacg gccgcctcgg tgacccgggt cggcgtcacg
     1561 gcgcgcaccg gcttcaggac cctggcgtcg ctcccgacga ggtagcggag cttggggcgg
     1621 cgggacgtcg ccacctgcac gaccttgcgc gcgacctccc ggggatccgc cttccgccgt
     1681 cgctccacct gcttcatggc gaaacgctcc atgcgctgcc cttcctcgta gtgcggggag
     1741 ctcgggtcga ccatcccctc ggcgcgcttg gcgttgtcga agaagatgtc cgtggcgtac
     1801 gtgcccggtt cgatgagcgt gacgaacacg ccgtcgtgga ccgtctccag gcggagggcc
     1861 tcgccgaacg cctccagcgc cgccttcgtc gcgttgtacg ccgacagcat gggggtcgag
     1921 ttgaagacgc cgatgctcga gatgaggatg atccgtccgg accggcgctc gcgcatcccg
     1981 gggagcaccg ccttcaccac acgtaccccg ccgaagaagt tcgtctcgaa ctgctcccgg
     2041 aactcctcca tggtggtgtc ctcgacgaac ccgccgaccc cgtagccggc gttgctgacg
     2101 aggacgtcca cgcga
//
LOCUS       DASPWO010000417         3210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_61000, whole genome shotgun sequence.
ACCESSION   DASPWO010000417 DASPWO010000000
VERSION     DASPWO010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61000"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VEO00_09525"
     CDS             complement(<1..253)
                     /locus_tag="VEO00_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYU58274.1"
                     /translation="MATGLLDALPEQERRLLRRVAHPDWVPPMLATLTDRRFSDPDWI
                     FERKLDGERALGFRDGTRVRLLSRNRKDLYDTYPEVVEAL"
     gene            complement(258..917)
                     /locus_tag="VEO00_09530"
     CDS             complement(258..917)
                     /locus_tag="VEO00_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HYU58275.1"
                     /translation="MAHTRTVWPGRFTGSPPPMGSLEEYRRKRTRRTPEPSARRGRAR
                     RHGMPRFVIQQHDATSMHWDVRLEAGGVLKSWAVPKGPSTDPRERRLAMPTEDHPLDY
                     AGFEGVIPPGEYGAGSVIVWDAGTYRNLTEDRGREVSVEEGVERGHVKVFLEGRKLRG
                     GYALTRTTAGARPRWILMKLKDEHADARRNPVRTEPRSVLSGVTIEEQAGRGRRRRSA
                     G"
     gene            complement(917..1531)
                     /locus_tag="VEO00_09535"
     CDS             complement(917..1531)
                     /locus_tag="VEO00_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYU58276.1"
                     /translation="MGRFRLTLTGVGAMNSPRFAPAGLLVERDRARIMLDGGPGSAPD
                     GPLQAWLVTDDRAELIREIRALAAERGVEPGVGAGNAAGLRIRPRKVVHTSHDTYGYL
                     VEAEGRRIAWAPEFWRFPSWAAGVDLLFADGAGWDRPIRFAGGVGGHACVEDVARRAP
                     ERGVRRLVFAHIGRPTIRAADAGLRPPFGVLGRDGQFVHPRRWR"
     gene            complement(1536..2258)
                     /locus_tag="VEO00_09540"
     CDS             complement(1536..2258)
                     /locus_tag="VEO00_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58277.1"
                     /translation="MQSTQEPPYTVVDTHAGGGINGGIGTVQEGTAPYVTMYAQTPDI
                     KASFDRAVALGAEVLIPVSDVPGIVTLCLLRDPQGNPFGMVTPGEGPGVSSGDGVPVD
                     WFEIIGPDPKALRDFYVELFGWERFGDDATGDYEYYQVHTGEGGLDGGIGSSPTGIRI
                     SRSMQRSTTCRSTSSVRRRSAAARPCRRPRWRTSRSRSSATRWATSSACTGEPSAAPR
                     VQIPGAPATGPSRGGERGDLRC"
     gene            complement(2443..>3210)
                     /locus_tag="VEO00_09545"
     CDS             complement(2443..>3210)
                     /locus_tag="VEO00_09545"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HYU58278.1"
                     /translation="WLRVQARALEGLDPQRAVARLRAHLADGPLRQRDLQGRLEAEGF
                     PRLALITASMWVDLVRVPPSGTWDQRRADLYGLADDWLGRAGRPAEAQALRHLIRRYL
                     GGFGPASLNDVAGWAGIPVSMLRPAAERLSLRRFRDEDGGKLLDLPRARLPDPDTPAP
                     ARFLPTWDATLLAHARRTRILPEPYRARVFDTKTPHSFPTFLVDGAVAGTWRYQGGRV
                     RLEPFGPLPARARRDLEAEAKRLAAFHADDGAIRAAP"
BASE COUNT          427 a         1210 c         1149 g          424 t
ORIGIN      
        1 cgagggcctc caccacctcg gggtaggtgt cgtagaggtc cttccggttg cgggacaaca
       61 gtcgcacccg cgtgccgtcc cggaacccca gtgcccgctc cccgtcgagc ttgcgctcga
      121 agatccagtc ggggtcggag aagcgccggt cggtgagcgt ggcgagcatc ggcggaaccc
      181 agtcagggtg ggccacccgg cgcaggagcc ggcgctcctg ctcggggagg gcgtcgagca
      241 ggccggtggc cattcgtcta cccggcgctc cggcgccgcc ggccgcggcc ggcttgctcc
      301 tcgatggtca ccccgctcag cacggagcgg ggctcggtgc ggaccgggtt gcggcgggcg
      361 tcggcgtgct cgtccttgag cttcatcagg atccagcggg gccgcgcgcc cgcggtggtg
      421 cgcgtcagcg cgtagccgcc gcggagcttc cggccctcga ggaacacctt gacgtgcccg
      481 cgttcgacgc cctcctccac ggagacctcc cggccgcggt cctcggtcag gttccggtac
      541 gtgccggcat cccagacgat caccgagccg gcgccgtatt cgcccggcgg gattacgccc
      601 tcgaagccgg cgtagtcgag cgggtggtcc tccgtcggca tggccaggcg ccgctcacgc
      661 gggtcggtgg acgggccctt cggcacggcc cacgacttca gcactcctcc ggcctccagt
      721 cgaacgtccc agtgcatcga ggtggcatcg tgctgctgga tgacgaaccg gggcattcca
      781 tggcggcggg cgcggccgcg gcgagcggac ggctccgggg tgcgccgagt ccgcttgcgc
      841 cggtattcct cgagcgaccc catcggcgga ggactgcccg tgaaccggcc tggccaaacc
      901 gtccgcgtat gtgccatcag cgccagcggc ggggatggac gaactggccg tcgcggccga
      961 ggaccccgaa gggaggcctg aggccggcgt ccgcggcccg gatcgtcggc cggccgatgt
     1021 gggcgaacac caggcgacgc acgccccgct ccggtgcccg gcgggcgacg tcttcgacgc
     1081 aggcgtgccc gcccacgccg ccggcgaacc ggatcgggcg gtcccacccg gcgccgtcgg
     1141 cgaacagcag atcgaccccc gccgcccatg acgggaagcg ccagaactca ggcgcccagg
     1201 cgatccggcg gccctccgcc tcgaccaggt agccgtacgt gtcgtgggag gtgtggacca
     1261 ccttccgcgg acggatgcgc aggccggccg cgttcccggc gccgaccccg ggctcgactc
     1321 cgcgctccgc ggccagcgcg cggatctccc ggatcagctc ggcacggtcg tcggtcacca
     1381 gccaggcctg gagcgggccg tccggggcgc tcccgggtcc gccgtcgagc atgatccggg
     1441 cccggtcgcg ctcgaccagc agcccggccg gagcgaatcg cggcgagttc atcgccccga
     1501 ccccggtcag cgtcagccgg aaccggccca cgccgtcagc atcgcaggtc ccctcgttcg
     1561 ccgccccggc tcgggcccgt ggccggggcg cccgggatct ggacccgagg cgccgcgcta
     1621 ggttcgccgg tacaggccga agaggttgcc caccgggtcg cggaactgcg cgaacgcgat
     1681 gttcgccatc tcggtcggcg gcatggccgt gctgccgccg agcgcctccg cacgctcgag
     1741 gtacttctgc aggtcgtcga ccgctgcata gagcgtgaga tacggattcc cgtcggggag
     1801 ctgccgatcc ctccgtccag cccgccctcg cccgtgtgca cctggtagta ctcgtagtcg
     1861 cccgtcgcgt cgtcgccgaa gcgctcccag ccgaagagct ccacgtagaa gtcccgtagc
     1921 gccttcgggt ccggtccgat gatctcgaac cagtcgaccg gcaccccgtc cccgctcgag
     1981 acgcccggcc cctcaccggg cgtcaccatg ccgaacgggt tcccctgcgg gtcgcgcagg
     2041 aggcagaggg tgacgatccc cggaacgtcc gacaccggga tcagcacttc ggcgccgagg
     2101 gcgaccgccc gatcgaagct cgccttgatg tccggggtct gcgcgtacat cgtcacgtac
     2161 ggagccgtcc cctcctggac ggtgccgatg cccccgttga tcccgcctcc cgcgtgcgtg
     2221 tcgaccacgg tgtagggagg ttcctgcgtc gactgcacgt gctagccgaa caggtcggca
     2281 tagaagcgcg ccagcgcctc tcgatcggga cccatcaact cgaagtgcac cacagggtct
     2341 gccatcgcga cctccttcct gaaacggccc gaggctacac cgatccggcg atgtggcaga
     2401 cgtgtcacgc ggagccccgc aaggccgggg ccgcacggcc cattacggcg ccgctctgat
     2461 cgcgccgtca tcggcgtgga acgccgccag gcgcttcgcc tcggcctcca agtcgcgacg
     2521 ggcgcgggcc gggagcggcc cgaacggctc gagccgcacg cggccgccct ggtagcgcca
     2581 cgttccggcc accgcgccgt ccaccaggaa cgtcgggaac gagtgcgggg tcttcgtgtc
     2641 gaagaccctc gcccggtatg gctcgggcag gatccgcgtc cggcgggcat gcgccagcag
     2701 ggtggcgtcc caggtgggca ggaaccgggc gggcgccggc gtgtccggat cgggcagccg
     2761 cgcccgcggc aggtcgagca gcttgccgcc gtcctcgtcg cggaatcgcc gcagcgacag
     2821 ccgttccgcg gcgggccgga gcatcgacac ggggatcccg gcccagccgg cgacgtcgtt
     2881 cagcgaggcc gggccgaacc cgcccaggta gcgtcggatc aggtgtcgca gggcctgcgc
     2941 ctcggccggc cgcccggccc gaccgagcca gtcgtcggcc agcccgtaga ggtcggcgcg
     3001 gcgctggtcc caggtgcccg acgggggcac ccgcaccagg tcgacccaca tcgaggcggt
     3061 gatcagggcg agccggggga acccctctgc ctccaggcgc ccctgcagat cgcgctggcg
     3121 cagcggcccg tcggccagat gcgcgcgcag tcgggcgaca gcccgctggg ggtccaggcc
     3181 ctccagcgcc ctggcctgga cccgcagcca
//
LOCUS       DASPWO010000418         3070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_482182, whole genome shotgun sequence.
ACCESSION   DASPWO010000418 DASPWO010000000
VERSION     DASPWO010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482182"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1828)
                     /locus_tag="VEO00_09550"
     CDS             complement(<1..1828)
                     /locus_tag="VEO00_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HYU58279.1"
                     /translation="MVGLLFAGGLVIGIPAADAYWQASTVLTHVRDATDGLKQVRTDL
                     LRGRVPTDESFAAATKGLGAAEEAMSGTRPTFGLIGHLPLIGLPVQATQRLVTATQHE
                     LQAAQVARDILEDVIGEPLPGSAAERARAEAETASGEATTPAERKQARKEHRRQGGKA
                     GGGTGRAKPPETNAILRDGKVDLKRLTALRPKVVELRAELAAAEEAVRGIGTVPFVSK
                     ANELRADIIEEVTLTHQLVDRGLTGLDLLPFVLGSDRDRSYLLLFQDSGYLRGTGGGY
                     FAFLDFSSRGGSLELVDSAAIIEIDRYRNEEVPIPKENWYLRTFPKSVRMNNLNWDPH
                     FPSTARVAANIYKEFTDRKVDGVIQIDVTGVAYLLDAIGQINVDAWPVPITSKNLAKI
                     ALQDSYVAFTDESDLEGDATSGAERKVFNEQLVTATWERLQSPKNVVRTVYQLSRALS
                     ERHLQIWSRDREEEDFFQELGWGGAIRKDPGDYVYVVDQNLGDDTLDTFAYERVDYDV
                     RIADDGSAEVVARVKMTNAVPRGLPFPIVPRDGPADKQTYVNLYAPGDAELDSVTQFE
                     RLRQRPVAKPLVHTELGRKVFSAKLQTKANESSYLEFRYRVPN"
     gene            complement(2558..>3070)
                     /locus_tag="VEO00_09555"
     CDS             complement(2558..>3070)
                     /locus_tag="VEO00_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYU58280.1"
                     /translation="EDSTVTLMTLHTAKGLEFPAVFIVGMEDGVFPHYRSMGDASQLE
                     EERRLAYVGITRARERLYLCHAWSRNLFGASNYNPPSRFLGEVPEGLVRLVEGRDALA
                     AEGDGGSGVYARGHIPALAAGDAVIHDRWGRGVVVAVSGNGTRTEATIEFPEQGEKRL
                     LLAYAPIRKA"
BASE COUNT          410 a         1100 c         1112 g          448 t
ORIGIN      
        1 cgttcgggac gcggtagcgg aactcgaggt acgacgactc gttcgccttg gtctgcagct
       61 tggcgctgaa gaccttccgt ccgagctccg tgtggaccag cggcttcgcg accggacgct
      121 ggcgcaggcg ctcgaactgc gtcaccgagt ccagctcggc gtcgcccggc gcgtagaggt
      181 tgacgtaggt ctgcttgtcg gccggaccgt cgcggggaac gatcgggaac ggcaggcccc
      241 tcggcacggc gttggtcatc ttcacccggg ccaccacctc ggcggagccg tcgtccgcga
      301 tccgcacgtc gtagtcgacg cgctcgtagg cgaaggtgtc gagcgtatcg tcgccgaggt
      361 tctgatcgac gacgtacacg tagtccccgg gatccttgcg gatcgcgccg ccccacccca
      421 gctcctggaa gaagtcctcc tcctcacggt cccggctcca gatctgcagg tggcgctcgg
      481 agagcgccct ggacagctgg tacacggtgc ggaccacgtt cttcgggctc tgcaaccgct
      541 cccaggtggc cgtgacgagc tgctcgttga acaccttgcg ttcggccccg gacgtggcgt
      601 cgccctcgag gtcgctttcg tcggtgaagg cgacgtagga gtcctgcagc gcgatcttcg
      661 ccaggttctt cgaggtgatc ggcaccggcc aggcgtccac gttgatctgc ccgatcgcgt
      721 ccagcaggta ggcgacgccg gtgacatcga tctggatcac gccgtcgacc ttgcggtcgg
      781 tgaactcctt gtagatgttg gccgcgaccc gggccgtgct cgggaagtgc gggtcccagt
      841 tcaggttgtt catccgcacc gacttcggga acgtgcgcag gtaccagttc tccttcggga
      901 tcggcacctc ctcgttcctg tagcgatcga tctcgatgat cgccgccgag tcgacgagct
      961 ccagcgaccc gccccgcgac gagaagtcca ggaaggcgaa gtagccgcct ccggtgccgc
     1021 gcaggtagcc actgtcctgg aacagcagga ggtaggagcg gtcgcgatcg gagccgagca
     1081 cgaacgggag gaggtcaagg ccggtcaggc cgcggtccac cagctggtgg gtcagcgtga
     1141 cctcctcgat gatgtccgcc cgcagctcgt tcgccttcga cacgaacggg acggtgccga
     1201 tgccgcgcac cgcctcctcg gcggcggcca gctcggctcg cagctcgacc accttcggcc
     1261 gcaacgcggt caggcgcttc agatcgacct tcccgtcgcg caggatggcg ttcgtctcgg
     1321 ggggcttggc gcggcccgta ccgcccccgg ccttcccacc ctgccggcgg tgctccttgc
     1381 gcgcctgctt gcgctccgcc ggggtagtgg cctcgccgct cgccgtctcg gcctccgcgc
     1441 gggcgcgctc cgctgccgat ccgggaagcg gctcgccgat gacatcttcc aggatgtcgc
     1501 gggcgacctg cgccgcctgc agctcgtgct gggtggccgt cacgaggcgc tgggtggcct
     1561 gcacggggag cccgatcagc gggaggtgcc cgatcagtcc gaacgtcggc cgcgtgccgg
     1621 acatggcctc ctctgccgcc cccagtccct tcgtcgcggc ggcgaaggac tcgtcggtcg
     1681 ggacccggcc gcgcagcagg tccgtgcgga cctgcttcag gccgtcggtg gcgtcacgga
     1741 cgtgcgtcag gaccgtggac gcctgccagt acgcgtcggc cgccgggatg ccgatcacca
     1801 ggccgccggc gaagagcagg ccgaccacca gcggccagcg ccgccgccgc cgcgacgcgt
     1861 agcggcgctc ccgctcgcgg gcccgttcgg cccgtccgcc gcgcgcctcg aagcgcgggg
     1921 gacccgagga cgagcgggac cgcgtgctcg tggacgatga gcgcgacgat ccggaacggg
     1981 acgaccccaa gcgtgtgggc tcgggccgcg actccgggcg ctcctccggc cccggccggg
     2041 gctccggggg ctcctccggc ccgggccggg gctccgggcg ctcgacgacg cgccgccgct
     2101 cggggggagc gggggcgagc gagacctcgc cgcttccggc cctctcgaag ggggcccaga
     2161 ccgacgtggc gggctcggcc agggcgggct cgtcgccgaa cagggcgtcc cactcgtctg
     2221 gtcccgccga gaggccgggc ggcttgtgcg ccgcgggtcg ggcgggcgaa ggagcggccg
     2281 gcgcgggagc gggagcgacg gcgctcgacg ggaccgcgct cgggctctcc gaggagcgac
     2341 gggggctcgg cccggcggac gtggatcgcg cggacgagcg cgacgcggac cgcgacgggg
     2401 agaggcggtc accgcgatgt gagcggtggt ggctggaacc gcggaacaac cggcggagtc
     2461 ggcgcagagg gcctctccat cggcgcttgc ggcgcccgcc cggccagagc acgggagatg
     2521 ggaggaaatc gggtggcacg agacccccga gtctaggtca tgcctttcgg atcggcgcgt
     2581 acgccagcag caggcgcttc tccccctgct cggggaactc gatcgtcgcc tcggtgcgcg
     2641 tcccgttccc cgagacggcc accaccacac cccgtcccca gcgatcgtgg atcacggcgt
     2701 cgccggccgc gagcgcgggg atgtgcccgc gagcgtacac gcccgaccct ccatcgccct
     2761 cggccgccag cgcgtcgcgg ccctcgacca ggcgcaccag gccctcgggg acctccccca
     2821 ggaaccggga cggggggttg tagttcgagg cgccgaacag gttccggctc caggcgtggc
     2881 acaggtacag gcgctcccgg gcccgcgtga tcccgacgta ggccagtcgc cgctcctcct
     2941 cgagctggga cgcatcgccc atggagcggt agtgcgggaa cacgccgtcc tccatcccga
     3001 cgatgaacac ggccgggaac tccagcccct tcgcggtgtg cagcgtcatc agcgtcacgg
     3061 tggagtcctc
//
LOCUS       DASPWO010000419         4070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1161176, whole genome shotgun sequence.
ACCESSION   DASPWO010000419 DASPWO010000000
VERSION     DASPWO010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161176"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VEO00_09560"
     CDS             complement(<1..929)
                     /locus_tag="VEO00_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58281.1"
                     /translation="MLDNEPTDTQYETTPVPGGPADAEQAPAKPARKRAASKRTANKT
                     AAGAAGAAPQEAVTQAAPSETAPEVAPAEAGPAPAKRAARKTTSRRTTKKAAAAPAGD
                     APGDAPAETPAVSETAVEGAAAGPQAPAKKTTRRRTTKKAAASGDTQTDAAAASAQAG
                     SAATTGTAVAEPVPSATAETAATETAPAETTADEPAGQRRRSRRGAATAAVLFQAPVA
                     APEPGRRRTARKVAEPEVAGETTADVVETPAEGAEEAAEAPQPRTRRTRQRRTQEARA
                     AEEAEAAEAAELEAADETTAEEGSAEEAAEEGA"
     gene            complement(1071..1868)
                     /locus_tag="VEO00_09565"
     CDS             complement(1071..1868)
                     /locus_tag="VEO00_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HYU58282.1"
                     /translation="MQKLRVRFAKRGRLRFTSHRDFQRAFERAVRRAGLPVAFSHGFS
                     PHPKISYAGAAPTGAASEAEYLEISLTSEQDPEQVRAALDEALPGGLDIVEVVVAGPG
                     SLADRLEASEWEIRLPQADPEAVAAAVEAFLAREEVLVERMTKRGMRTFDCRAAVLRL
                     TVGLADAATEPSLEGCAILQAVVRHGTPTVRPDDVLAGVLAVATLSMTGPALLTRLAQ
                     GPLDDGTGTVDDPLARDRDATQATATEPVDAHETHPAEGDAAAPPTP"
     gene            2011..2835
                     /locus_tag="VEO00_09570"
     CDS             2011..2835
                     /locus_tag="VEO00_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYU58283.1"
                     /translation="MVDTQPVPSEEPERALPDTSAGAGDDHDANDGKGFYWVLKNVVL
                     GPPIKRVFRPKVEGLENVPRHGPAIIASNHLSYADWLFMPLAAPRRVTFVAKADYFVG
                     RGLKGSFQRGFFKGTGQVPIDRSGGSASEGALRAGRKVLERGELFGIYPEGTRSHDGR
                     LYKGRTGVARLALESKVPVIPCGVVGTDKVAPPGKVIASLASPTVRFGEPLDFSRYDG
                     MERDRLILRAVTDEIMYRIMELSGQEYVDMYATKAKALEGRATTGAPKKVRRSASE"
     gene            2858..3754
                     /locus_tag="VEO00_09575"
     CDS             2858..3754
                     /locus_tag="VEO00_09575"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HYU58284.1"
                     /translation="MNGPIAYQGEPGANSAVACAEMFPGREQLACTTFEDAIDAVTNG
                     DAELAMIPIDNSIAGRVADIHHLLPESGLHIVGEHFLPIHFQLMAPPGATQDGVRTVR
                     SHVHALGQCRKIVREHGWSTVVSDDTAGAAREVAELGDPTVAALAPRAAAELYGLRIL
                     ATDVEDEHHNTTRFLALSREPQVPDRAGSPAITTFVFRVRNVPAALYKALGGFATNGV
                     NMTKLESYQLGGTFFATQFYADVEGHPEDPGVALALEELAFFSVEVRVLGVYPAHPYR
                     AEIAEPDANRVNHPHPIDQSHL"
     gene            3795..>4070
                     /locus_tag="VEO00_09580"
     CDS             3795..>4070
                     /locus_tag="VEO00_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU58285.1"
                     /translation="MQIIRVDGVDAAQFARFHELIRTVRGRDSAYPTVMGLDEARVLF
                     TGNHRDIRRSGLAAVDGDAWLGVAWLDEELLENTDILEVEVVVEHAYR"
BASE COUNT          514 a         1509 c         1465 g          582 t
ORIGIN      
        1 tcggcgccct cctcggcagc ttcctccgcg ctgccctctt cggcggtggt ctcgtcggcg
       61 gcctcgagct cggcggcctc cgccgcctcg gcctcctcgg cggccctggc ctcctgggta
      121 cgacgctggc gggtgcggcg agtgcgcggc tgcggcgcct ccgcggcttc ctcggcgccc
      181 tccgccgggg tctcgacgac gtccgcggtg gtctccccgg cgacctccgg ctcggcgacc
      241 ttcctggccg tacgacgacg gcccggctcc ggtgcggcga cgggggcctg gaacagcacc
      301 gcagcggtcg cggcgccccg ccgcgaacgc cgccgctgac ccgccggctc gtccgccgtg
      361 gtctcggccg gcgccgtctc ggtcgctgcg gtctcggcgg tcgcgctcgg gaccggctcg
      421 gctacggcgg tgccggtcgt ggcggcgctg ccggcctgcg cggaggcggc ggccgcgtcg
      481 gtctgggtgt cgccgctcgc cgcggccttc ttcgtcgtac gacgccgggt ggtcttcttc
      541 gccggcgcct gcggcccggc ggcggcgccc tcgacggcgg tctcgctcac cgccggggtc
      601 tcggccgggg cgtcgcccgg cgcgtcaccg gccggcgcgg cggcggcctt cttcgtcgta
      661 cggcgactgg tggtcttcct ggccgcgcgc ttcgcgggcg ccgggccggc ttcggccggc
      721 gccacctcgg gcgcggtctc ggatggagcg gcctgggtca ccgcctcctg cggcgccgcg
      781 ccggcggcgc cggcggcggt cttgttcgcg gtccgcttgg aggcggcgcg cttgcgggcc
      841 ggcttggccg gcgcctgctc ggcgtcggcg ggcccgcctg ggacgggcgt ggtctcgtac
      901 tgggtgtcgg tcggctcgtt gtcgagcatg tggtgcggtc tcctcaggct cccagcgtgc
      961 ccactctgga ccctggcaag ggtccggcac agccggaagc ggtctcggtc gccgctccgg
     1021 acggcggtgt cggagcggcg gaagcctgct ggatccgctc ccggcgcctg ctaaggcgtc
     1081 ggcggagcgg cggcgtcgcc ctccgctgga tgcgtctcgt gggcgtccac cggctcggtc
     1141 gcggtcgcct gggtggcgtc gcggtcacga gccaacggat cgtccacagt gccggtgcca
     1201 tcgtcgagcg ggccctgcgc gagcctggtc aacagagccg ggcccgtcat cgagagcgtc
     1261 gctacggcga gcacgccggc gagaacgtcg tcgggtcgta cggtcggggt tccgtgccgt
     1321 actaccgctt gcagtatcgc acacccttcc agtgacggct cagttgccgc gtccgcgagt
     1381 cccacggtga gccgcagtac ggcggcccgg cagtcgaacg tccgcatccc ccgcttggtc
     1441 atccgctcga ccagcacctc ctcgcgcgcc aggaaggcct cgaccgccgc cgcgaccgcc
     1501 tccggatccg cctgcgggag cctgatctcc cactcactgg cctccagccg gtcggccagc
     1561 gaaccgggcc cggcgaccac cacctcgacg atgtccagac cgcccggaag tgcctcgtcg
     1621 agggccgcgc ggacctgctc cgggtcctgc tcggatgtga gcgagatctc caaatactcg
     1681 gcctcgctgg cggcaccggt gggcgcagcc ccggcgtacg agatcttcgg gtgcgggctg
     1741 aaaccgtgcg agaacgccac cggcagcccg gcccgccgga ccgcgcgctc gaacgcgcgc
     1801 tggaagtccc ggtgcgaggt gaaccgcagc cgcccccgct tggcgaacct cacccgcagc
     1861 ttctgcacgg cgggaagctg ctgcgacggc ggagcttgaa caggtgccac aaccgcttag
     1921 tgtctcatcc acacgccgtc gggccggagc ggcgccgggt gggcgtcgta cggcatcatg
     1981 agggttcggg gacgcgagga gaggacaacg atggtcgaca cgcagccggt gccgtcggag
     2041 gagcccgagc gggcgctgcc ggacacgtcg gccggcgcgg gggacgacca cgacgccaac
     2101 gacggcaagg gcttctactg ggtgctcaag aacgtcgtcc tcgggccgcc gatcaagcgg
     2161 gtgttccggc cgaaggtcga ggggctggag aacgtgccgc ggcacggccc ggcgatcatc
     2221 gccagcaacc acctgtcgta cgcggactgg ctgttcatgc cgctcgcggc cccccgccgg
     2281 gtgaccttcg tggcgaaggc cgactacttc gtcgggcgcg gcctgaaggg gtcgttccag
     2341 cgcgggttct tcaagggcac cgggcaggtc ccgatcgacc gcagcggcgg ctcggccagc
     2401 gagggcgcgc tgcgcgcggg acgcaaggtg ctggagcgcg gcgagctgtt cgggatctac
     2461 cccgagggca cccgctcgca cgacggcagg ctctacaagg gccgcaccgg cgtcgcccgc
     2521 ctcgccctgg agtccaaggt cccggtgatc ccgtgcggcg tggtcggcac cgacaaggtc
     2581 gccccgcccg gcaaggtcat cgcgtcgctc gcctccccca cggtccgctt cggcgagccg
     2641 ctcgacttct cgaggtacga cgggatggag cgcgaccgcc tcatcctgcg tgccgtcacc
     2701 gacgagatca tgtaccggat catggagctg tccggccagg agtacgtcga catgtacgcc
     2761 accaaggcca aggcactcga aggccgcgcc actaccggcg ccccgaagaa ggtccgcagg
     2821 tcggcatccg agtgaagcgg cggtagagtc acagaccgtg aacggaccta tcgcctatca
     2881 gggcgagcct ggtgccaact cggcggtggc ctgcgcggag atgttcccgg ggcgtgagca
     2941 gctcgcgtgc acgacgttcg aggacgcgat cgacgcggtc acgaacggcg acgcggagct
     3001 ggccatgatc ccgatcgaca actcgatcgc cggccgggtc gcggacatcc accacctgct
     3061 gcccgagtcg gggctgcaca tcgtgggcga gcacttcctg ccgatccact tccagctgat
     3121 ggccccgccg ggcgccacgc aggacggcgt ccgcaccgtc cgcagccacg tgcacgcgct
     3181 cggccagtgc cgcaagatcg tccgcgagca cggctggagc accgtcgtca gcgacgacac
     3241 cgccggcgcc gcccgcgagg tcgccgagct cggcgatccc accgtcgccg ccctcgcgcc
     3301 acgcgccgcg gcggagctgt acggcctccg catcctcgcg accgacgtcg aggacgagca
     3361 ccacaacacc actcgcttcc tcgcgctctc gcgggagccg caggttcccg accgcgccgg
     3421 cagcccggcc ataacgacct tcgtgttccg cgtgcgcaac gtgccggccg cgctctacaa
     3481 ggcgctaggc gggttcgcga ccaacggggt caacatgacc aagctggaga gctaccagct
     3541 cggcggcacg ttcttcgcga cccagttcta cgccgacgtc gagggccacc cggaggaccc
     3601 gggcgtggcg ctcgcgctgg aggagctggc cttcttctcg gtcgaggtac gcgtactggg
     3661 cgtctacccg gcacacccct accgcgccga gatcgccgag ccggacgcga accgggtcaa
     3721 ccacccgcac ccgatagacc agtcccacct gtaaaccagt cgcctcgccc ggcgggtcgt
     3781 cgtaccgtcc gggcatgcag atcatccgtg tcgacggggt cgacgccgcg cagttcgccc
     3841 gcttccacga gctgatccgc acggtgcgcg gccgcgacag cgcgtatccg acggtgatgg
     3901 ggctggacga ggcgcgggtg ctgttcaccg ggaaccaccg cgacatccgc cggtccggcc
     3961 tggccgccgt cgacggggac gcctggctcg gcgtcgcgtg gctggacgag gagctgctgg
     4021 agaacaccga catcctcgag gtcgaggtcg ttgtcgagca cgcgtaccgg
//
LOCUS       DASPWO010000420         4531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_142371, whole genome shotgun sequence.
ACCESSION   DASPWO010000420 DASPWO010000000
VERSION     DASPWO010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142371"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VEO00_09585"
     CDS             complement(<1..531)
                     /locus_tag="VEO00_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58286.1"
                     /translation="MSTDRNQMRRRVLFAAAGAGLALVVVASPAGAGVGDFFKLGQTN
                     TADATSFLTGNTAGPELRVSNANGNFAGIKAESAGGYGAAVYGLHSSEGGTGAGVQGI
                     SLSTENSAVGVYGLIDSTTPGAGSAAVRGQNNATGFSGYGVYGFHAGTGIGVSGRSPK
                     GLGVYGNGTRGGSFYGT"
     gene            complement(584..1042)
                     /locus_tag="VEO00_09590"
     CDS             complement(584..1042)
                     /locus_tag="VEO00_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58287.1"
                     /translation="MAALVAALGLLVGMPSVASAEAVTGTTTFHDATQSFQDINPCTG
                     DPVTVTVNYDGVFSYAVDPQGGVHVTGTTAGTFEVVPFDPSLPTYTGHFASWFGESSS
                     ANSDGDWVTLNIKLRGSDGSMISMNVVVQFHLSGGVLHVGFDKERLRCGS"
     gene            1311..2444
                     /locus_tag="VEO00_09595"
     CDS             1311..2444
                     /locus_tag="VEO00_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYU58288.1"
                     /translation="MREELVSFIRRHGDETLASALTVVVLVGVAVQDEPAGARLASAA
                     AAIALGAAAARRTRRPLVFLGLLVLITVASALVPHPSDTAGAFLLFLLLAVYSAATHT
                     SGRRTVVAGLLTVVIYVTDLAMDPEPISPEGVIFYALLFGAPWVTGRAVRQRRLHQRR
                     LEQEKARAETAIVEERARIARELHDVVAHSISVMVLQARGGRRVIDAEPADAREAFAT
                     IERTGHHALEEMRRLLGMLRRSDEELALAPQPSLKQLDTLVEQVQAAGLPVEVTVEGE
                     PRELPPGVDLSAYRIVQEALTNALKHAGPARARVLLRYCPDDLELEIADDGPGTGDGS
                     GSGQGLIGMRERVSVYGGELQAGRRPEGGYALRARLPLGSART"
     gene            2441..3097
                     /locus_tag="VEO00_09600"
     CDS             2441..3097
                     /locus_tag="VEO00_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU58289.1"
                     /translation="MTIRVLLADDQQLVRGGFRMILRAEPDIEVVGEAADGAEAVALA
                     RELDPDVVLMDVRMPNMDGIEATRRIIDGSDEAPRVLVLTTFDLDEYVYEALRAGASG
                     FLLKDAPEEQLVSGIRIVADGGSLFAPAVTRRLIERFAGIAPRNPPTALAELTPRELE
                     VLRLLARGLSNAEISAELFVSEHTAKTHVAHILNKLDLRDRVQAVVLAYESGIVRAGG
                     "
     gene            complement(3138..4055)
                     /locus_tag="VEO00_09605"
     CDS             complement(3138..4055)
                     /locus_tag="VEO00_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYU58290.1"
                     /translation="MHDEAQSRLVGIGDTELYVVERGAADGYPLILLHGGPGDDHHEF
                     GGYLDPLGDRYRLILVDQRGQGRSGESNPSMWTLQQMAADVVLLARALRLERYAVLGH
                     SYGAFVTLQNAVDFPGMAWQSIVSGGLPSARWMADLDAKLAAFEPEDLRERVQRSWEK
                     ESQVRTEEDFAAIMHEQMPWHFADPRDPRIEEYERRTAGAKYAPDVLRVFAQADYGGI
                     ELEDRLGEIAHPVLVLAGRHDRTCPVEASELMAKEIPGAELVVLEHSGHMSFVEEPDR
                     YVEAVRDFLDRHTAQEDPGSSTPTDVASG"
     gene            complement(4048..>4531)
                     /locus_tag="VEO00_09610"
     CDS             complement(4048..>4531)
                     /locus_tag="VEO00_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HYU58291.1"
                     /translation="VTVPYAGQADTYRVLKDHLPEGRIVCDCTSPLATAVGGRPWQVI
                     RPWHGSAVEQAEAIVGRGRLRLVSGFQTVSGELLQDIERPMEGDVLLCGSDAEAKATV
                     GSLVDDIPEMRWADAGELSMARLIEPLTAVLVSLNRTYKLKDTGIALSGRDHWGRPGA
                     "
BASE COUNT          646 a         1658 c         1546 g          681 t
ORIGIN      
        1 ggtgccgtag aacgagccgc cgcgcgtccc gttcccgtac acgccgagcc ccttggggct
       61 ccgcccgctg acgccgatgc cagtgcccgc gtggaagccg tagacgccgt agccgctgaa
      121 cccggtggcg ttgttctgac cgcggacggc ggcggagccg gcccccggcg tggtcgagtc
      181 gatcaggccg tagaccccga ccgcagagtt ctcggtcgac agggagatcc cctgcacgcc
      241 cgccccggtt cccccctccg agctgtgcag gccgtacacc gccgcaccgt atccgcccgc
      301 gctctccgcc ttgatcccgg cgaagttgcc gttcgcgttg ctcaccctca actccgggcc
      361 ggcggtgttg ccggtcagga agctggtggc gtcggcggtg ttggtctggc cgagcttgaa
      421 gaagtcaccg accccggcgc ccgcgggcga cgcgaccacg accagggcaa gccctgcccc
      481 cgcggccgcg aacagaacac gccgccgcat ctggttcctg tccgtgctca tcgtttctcc
      541 cttctcgctt gctacggacg gctcaacgag ccaagcgcca gcgtcagctc ccgcagcgca
      601 gccgctcctt gtcgaatccg acgtggagga cgccgcctga gaggtggaat tggaccacca
      661 cgttcatgct gatcatcgag ccgtccgacc cgcgcagctt gatgttcaac gtgacccaat
      721 cgccgtcgct gttggcggag gaactctctc cgaaccaact ggcgaaatga ccggtgtacg
      781 tggggaggct cgggtcgaag ggaacgacct cgaaggtccc ggcagtggtc cccgtcacgt
      841 gcacccctcc ctgcggatcg accgcgtagg agaacacccc atcgtagttc acggtgacgg
      901 tgacgggatc ccccgtgcac ggattgatgt cctggaagct ctgcgtggcg tcgtggaacg
      961 tggtcgtccc cgtcaccgct tcggcggagg cgacggaggg catgcccacg agcagcccga
     1021 gcgccgcgac aagcgccgcc acgacgaaga tccaacgagc gagccccgaa cgcggcggct
     1081 ccggcaaccg accgtgcctc gtcccctgga gcttcatgcg cctcactcct tcctgacccg
     1141 atgggccgat gtgacttaag ggatgtcacg gccgaccgta gaggccggcg ccgagctcgt
     1201 cgtcgccgtc ccaggtgaat ctggctcgct ctcgagagcg atctcggcgt acgtggatca
     1261 ctctcacggc ggatggaaac gggagcgtga acgccgtagc gtcagcttcg atgcgggagg
     1321 aactcgtgag cttcatccgg cggcacggtg atgagacgct ggccagcgcg ctcaccgtgg
     1381 tcgtgctggt cggggtcgcg gtgcaggacg aaccggcggg ggcccggctg gcatcggccg
     1441 ccgccgccat cgccctgggg gccgccgcgg ctcgaaggac ccgcaggcca ctggtgtttc
     1501 tcggcttgct ggtcctcatc accgtcgcgt ccgccttggt accgcatccc tcggatacgg
     1561 ccggtgcgtt cctcctgttc ctcctgctgg cggtgtacag cgctgccacg cacacgagcg
     1621 gacgccggac ggtggtcgcc ggcctcctga ccgtcgtcat ctacgtcacc gacctcgcca
     1681 tggacccgga gccgatctcg cccgagggcg tgatcttcta cgcgctcctg ttcggcgccc
     1741 cctgggtgac cggccgtgcc gtccgccaac ggcgcctcca ccagcggcgt ctcgaacagg
     1801 agaaggcgag ggcagagacg gcgatcgtgg aggaacgcgc gcggatcgcc cgtgagctcc
     1861 acgacgtggt cgcccactcg atcagtgtca tggtcctcca ggcccgcggc ggccggcgcg
     1921 tcatcgatgc cgagcccgcc gacgcgcggg aggcgttcgc caccatcgag cgcacgggcc
     1981 accacgcgct cgaggagatg cgccgcctgc tcggcatgct ccggaggagc gacgaggagc
     2041 tcgcgctcgc gccacagccc agcctgaagc agctcgacac gctggtggag caggtccagg
     2101 ccgccgggct gcccgtcgag gtgacggtcg aaggggagcc gcgggagctg ccgccgggcg
     2161 tcgatctctc cgcttaccgg atcgtccagg aggccctcac gaacgccctc aagcacgccg
     2221 gccccgctcg cgcccgggtc ctcctccggt actgcccgga cgacctcgag ctcgagatcg
     2281 cggacgacgg tcccggcacc ggagacggtt cgggttccgg gcaggggctg atcgggatgc
     2341 gcgagcgcgt gtccgtctac ggcggcgagc tgcaggcggg acggcgcccc gaaggcggct
     2401 acgcgctgcg ggcccggctg ccgctcggct ccgcacggac atgaccatcc gggtcctcct
     2461 cgccgacgac cagcagctcg tccgcggggg gtttcgcatg atccttcggg ccgaaccgga
     2521 catcgaggtg gtcggcgagg ccgccgatgg cgcagaggcg gtcgcgctcg cccgggagct
     2581 ggacccggac gtcgtgctca tggacgtgcg catgccgaac atggacggca tcgaggcgac
     2641 ccgccggatc atcgacggca gcgacgaggc gccccgtgtc ctcgtgctca cgaccttcga
     2701 cctcgacgag tacgtctacg aggcgcttcg ggccggcgcg agcggcttct tgctcaagga
     2761 cgcgcccgag gagcagctcg tgtcggggat caggatcgtc gccgacggag ggtcgctgtt
     2821 cgcccccgcg gtgacccggc ggctgatcga gcgcttcgcc ggcatcgcgc cgcggaaccc
     2881 tccgaccgcg ctcgccgagc tcacaccgag ggagctcgag gttctccgcc tgctggcccg
     2941 cggtctgtcg aatgcggaga tctccgcgga actcttcgtc tccgagcaca ccgcgaagac
     3001 ccacgtcgcg cacatcctca acaagctcga cctgcgcgac cgggtccagg cggtcgtcct
     3061 cgcgtacgag tccgggatcg tccgcgccgg cggataggtg cccgcccgga agcgctggag
     3121 ccgggccgtc gctcgaccta tccggaggcg acgtcggtcg gcgtgctgct gccggggtcc
     3181 tcctgggcgg tgtggcggtc caggaagtcg cgaacggcct ccacgtagcg gtccggctcc
     3241 tcgacgaacg acatgtggcc gctgtgttcg agcacgacga gctcggcgcc ggggatctcc
     3301 ttcgccatga gttccgaggc ctccacggga caggtgcggt cgtgccgccc ggccagcacc
     3361 agcacggggt gcgcgatctc gccgaggcgg tcctcgagct cgatcccccc gtagtcggcc
     3421 tgcgcgaaga cgcgaaggac gtcgggcgcg tacttcgccc ccgccgtgcg ccgttcgtac
     3481 tcctcgatgc gagggtcgcg gggatcggcg aagtgccagg gcatctgctc gtgcatgatc
     3541 gcggcgaagt cctcctccgt tcgaacctgg ctctccttct cccacgaccg ctggacgcgc
     3601 tcgcggaggt cctctggctc gaacgccgcc agcttcgcgt cgaggtcggc catccacctc
     3661 gcggagggca gcccgcccga cacgatcgac tgccacgcca tgccggggaa gtccacggcg
     3721 ttctggagcg tgacgaacgc gccgtaggag tgcccgagca ccgcgtaccg ctccagccgg
     3781 agcgcccggg ccagcagcac gacgtcggcc gccatctgct ggagggtcca catcgagggg
     3841 ttcgattccc ccgagcgccc ctgtccccgc tggtcgacca ggatcagccg gtaccggtcg
     3901 cccagcgggt ccagatagcc gccgaactcg tggtggtcgt cgccggggcc gccgtgcagc
     3961 aggatcagcg ggtacccgtc tgccgcgccc cgctcgacga cgtacagctc ggtgtcgccg
     4021 atcccgacca gccgcgactg cgcctcgtca tgcacccggc cggccccagt ggtccctgcc
     4081 ggacagggcg atgccggtgt ccttcagctt gtacgtcctg ttcaggctga ccagcaccgc
     4141 cgtgagcggc tcgatgagcc tcgccatcga cagctcgccc gcgtcggccc agcgcatctc
     4201 ggggatgtcg tcgacgagcg aacccacggt ggccttggcc tctgcgtcgg acccgcacag
     4261 cagcacgtcg ccctccatgg gccgctcgat gtcctggagc agctcccccg acacggtctg
     4321 gaacccgctg accagccgca gccggccccg cccgacgatg gcctcggcct gctcgacggc
     4381 ggagccgtgc cacggccgga tcacctgcca gggccggccg ccgaccgccg tggccagcgg
     4441 cgacgtgcag tcacacacga tccgcccctc cgggaggtgg tccttgagga cgcggtaggt
     4501 gtccgcctgc ccggcgtacg ggaccgtcac g
//
LOCUS       DASPWO010000421         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_230661, whole genome shotgun sequence.
ACCESSION   DASPWO010000421 DASPWO010000000
VERSION     DASPWO010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230661"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VEO00_09615"
     CDS             <1..752
                     /locus_tag="VEO00_09615"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HYU58292.1"
                     /translation="DLDLFSDAPPGSGLGSSSALVVAMLAAVAAHAREPVTPYELAGR
                     AYTVERVDLRQAGGMQDQYAAAFGGFNFIEFLGQDRVVVNPLRIDPNVENELHGSLLL
                     CDTRMTRRSGGILKRQVEGYTSGRAESLRSLARIKELTVELKEALLTGNLARFGEGLS
                     ENWEAKRRLAEGISTDRIEELYEIGLKAGAVGGKVLGAGGGGHLLLFCPFRQRPAVAR
                     AMEVAGARIVRFHFEARGVQTWRARAEEEAP"
     gene            749..1369
                     /locus_tag="VEO00_09620"
     CDS             749..1369
                     /locus_tag="VEO00_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HYU58293.1"
                     /translation="MTDASRAPGEAFEAAALEYLETSARHLAALVSPETAAAAARAGH
                     VLAEALQGGGKVLFCGNGGSAADAQHLAAELVGRLSGDRDREPLAAISLTTDASILTA
                     LSNDYGYPDVFARQVMALGRPGDALVAISTSGRSENVLRAARIAAEGGMRVVGLAGPD
                     LSPLDDLVDICLHAPGETSGLVQQGHITLGHLLCALAERAILDEES"
     gene            1366..1929
                     /locus_tag="VEO00_09625"
     CDS             1366..1929
                     /locus_tag="VEO00_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYU58294.1"
                     /translation="MRGDRPAVFLDRDGTLIEDPGHLGDPDGVVLLPGVAAALRRLAA
                     ASFALVVVSNQAGVARGIFTEAAVRAVNRRLGSLLDGDGVHLDAFYVCPHHPEFTGPC
                     ECRKPGTKMLEDAAAELGLDLGASWIVGDQGTDAEAGRRAGARPVLVRTGDGTHEAVR
                     VPRGVPVVADLAVAAALILGDRPVSRG"
     gene            complement(1916..>2564)
                     /locus_tag="VEO00_09630"
     CDS             complement(1916..>2564)
                     /locus_tag="VEO00_09630"
                     /inference="COORDINATES: protein motif:HMM:NF020683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HYU58295.1"
                     /translation="GDRGVTNTADIIDLTAPKPAWRPTAPMHFRRESANAVLLLDGTV
                     LVTGGRSDFSTSRRAEIFDPVTETWSVMAAAKAGRQYHSTSVLLPDGRVLSAGEDFGP
                     YRETGELFAPPYLFKGVRPRITSAPALAHYGQSLAVSFADASHISRVALVRPGSVTHS
                     VNFDQRYVDLSYSVNGSSITATAPASGRLAPPGWYMLTVLNEKAVPSEAAWVNLG"
BASE COUNT          329 a          921 c          980 g          334 t
ORIGIN      
        1 tcgacctcga cctgttctcc gacgccccgc cggggtcggg cctggggtcc tcatcggcgc
       61 tcgtcgtcgc gatgctcgcc gccgtggccg cgcacgcccg agagccggtg acgccgtacg
      121 agctcgcggg gcgggcgtac accgtcgagc gggtggacct gcgccaggcc ggggggatgc
      181 aggaccagta cgcggcggcc ttcggcggct tcaacttcat cgagttcctc ggccaggacc
      241 gggtggtggt caacccgctg cgcatcgacc ccaacgtgga gaacgagctg cacgggtcgc
      301 tcctgctctg cgacacgagg atgacccgga ggtcgggcgg catcctgaag cggcaggtcg
      361 agggctacac ctccggccgc gccgagtcgc tccggtcgct ggcgcggatc aaggagctga
      421 ccgtcgagct gaaggaggcc ctgctgaccg gcaacttggc gcggttcggc gaggggctgt
      481 cggagaactg ggaggccaag cgccggctgg cggaaggcat ctcgaccgac cgcatcgagg
      541 agctgtacga gatcgggctg aaggccggcg cggtcggggg gaaggtgctc ggcgccggcg
      601 gcggcggcca cctgctgctg ttctgcccct tccggcagcg cccggccgtg gcccgggcga
      661 tggaggttgc cggcgcgcgg atcgtgcgct tccacttcga ggcccgcggc gtccagacct
      721 ggcgcgcgag ggcggaggag gaggccccgt gaccgacgcg agccgggcgc ccggggaagc
      781 gttcgaggcg gccgccctgg agtacctcga gacctccgcc cgccaccttg cggccctggt
      841 gagcccggag accgccgcgg cggcggcgcg ggccggccac gtgctggccg aggccctgca
      901 gggtggcggg aaggtcctgt tctgcgggaa cggtgggagc gccgccgacg cgcagcacct
      961 ggcggccgag ctggtcgggc ggctctccgg ggaccgcgac cgcgagccgc tcgcggcgat
     1021 ctcgctgacc accgacgcct cgatcctcac cgccctctcg aacgactacg gctacccgga
     1081 cgtgttcgcg cgccaggtga tggcgctcgg gcgtccgggc gacgcgctgg tcgccatctc
     1141 gaccagcggc cgctccgaga acgtgctccg ggccgcccgc atcgccgcag agggcgggat
     1201 gcgtgtcgtc gggctggccg gacccgacct ctctccgctc gacgacctgg tcgacatctg
     1261 cctgcacgcc cccggcgaga ccagcggcct ggtccagcag ggccacatca cgctcgggca
     1321 cctcctctgc gccctggccg agcgggccat cctcgacgag gagtcgtgag gggcgatcgc
     1381 ccggcggtct tcctcgaccg cgacgggacg ctgatcgagg acccgggcca cctcggcgat
     1441 ccggacggtg tggtgctcct gcccggcgtg gcggccgccc tgcgccggct cgccgcggcc
     1501 agcttcgcgc tcgtcgtcgt ctcgaaccag gccggcgtgg cgcgcggcat cttcaccgag
     1561 gccgcggtgc gcgccgtgaa ccggcggctc ggatcactgc tcgacggcga cggggtgcac
     1621 ctcgacgcgt tctacgtctg cccgcaccac ccggagttca ccgggccgtg cgagtgccgc
     1681 aagcccggga cgaagatgct ggaggacgcc gcggcggagc tcggcctcga cctcggcgcc
     1741 tcctggatcg tcggcgacca gggcaccgac gccgaggccg gccggcgcgc cggcgcccga
     1801 ccggtgctcg tgcgcaccgg cgacgggacg cacgaggcgg tccgcgtccc ccgcggcgtc
     1861 ccggtcgtcg ccgacctcgc cgtggcggcc gccctgatcc tcggcgaccg tccggttagc
     1921 cgaggttgac ccaggccgcc tcggagggca cggccttctc gttcagcacg gtgagcatgt
     1981 accagcccgg cggcgccagg cggccgctcg ccggggccgt ggcggtgatc gacgacccgt
     2041 tcaccgagta ggacaggtcc acgtagcgct gatcgaagtt gacggaatgc gtgaccgagc
     2101 ccggccggac cagggcgaca cggctgatat gcgaggcgtc ggcgaacgag acggccaggg
     2161 actgcccgta gtgcgccagc gccggcgcgg aggtgatccg cggccggacg cccttgaaca
     2221 ggtagggcgg cgcgaacagc tcgccggtct cgcggtacgg gccgaagtcc tccccggccg
     2281 acagcacgcg gccgtcgggg agcaagaccg aggtggagtg gtactggcgt cccgccttgg
     2341 cggcggccat cacgctccag gtctcggtca ccgggtcgaa gatctccgcc cggcgggagg
     2401 tcgagaagtc cgagcgcccg ccggtgacga gcacggtccc gtcgagcagg aggacggcgt
     2461 tcgccgactc acgccggaag tgcatcggcg ccgtcggccg ccaggccggc ttcggcgccg
     2521 tgaggtcgat gatgtcggcg gtgttggtga cgccgcggtc cccg
//
LOCUS       DASPWO010000422         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_402686, whole genome shotgun sequence.
ACCESSION   DASPWO010000422 DASPWO010000000
VERSION     DASPWO010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402686"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2026
                     /locus_tag="VEO00_09635"
     CDS             <1..>2026
                     /locus_tag="VEO00_09635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU58296.1"
                     /translation="DRDVERAFVPLFHELLDSFTHQQFDPDRFARVLSALRAHFEVWD
                     YAATWRSRAVWEAFRERAVSSNQVLGDGGLIAATWSLPDPAGPDAQAATVGETTEALR
                     FLYRALSPVTAELPVADVTHATSSGMAAIPGILAAVERRTAMIVSEPTLYLRDQYLTL
                     SRHGLPFPLKRLLLRFSSAMAQTAYHFADRVAPACEWGGRWEQAYGVPPDRIEVIYPG
                     VDQDAFRPMPMDRSERPTVVSLSPMEPREDLETLLDVADRVRRAVPDVLFMHHGPAVD
                     GPYSERIRALARDRLLEDTVKFMGPTDRVAAALASADLMVSTTTSETFPLAVVESLAC
                     GTPVVATAVGGVHEALEGPGLTAPPGDAERLAEAVVAILRLPDEQRQLLAEAARSIAT
                     QKFGLSTFVERYREAYVELWGVPVEAVPAARKEEVPMGVAVAKREIEVQAEAEAEVPA
                     AASPVPAAAPRVPAAAPRVPAAAFVRTPAAAAAAIPPAPPMPRERRAEPAPSVAPPTP
                     ARRLVPAAAATPGRRPLLWEVPAPAPAGAAAFSGPLRAGSADRAVTPTPTPTPSRPAP
                     TPARPNTPSVRKLSTSRPPAEPTAAPAAPPRLVPVPNGMAPSELGLALESEDPFARVG
                     ALSRAGGEPGLADVVATALADDYPQVRREAVRALQRIGGPLAGRALADAVA"
BASE COUNT          234 a          800 c          722 g          270 t
ORIGIN      
        1 gaccgcgacg tggagcgggc gttcgtcccc ctgttccacg agctgctcga ttcgttcacc
       61 caccagcagt tcgaccccga ccggttcgcc cgcgtgctct cggcgcttcg ggcccacttc
      121 gaggtgtggg actacgcggc gacgtggcgg tcccgggccg tgtgggaggc gttccgggag
      181 cgggccgtct cttcgaacca ggtcctgggc gacggcggcc tgatcgccgc gacgtggagc
      241 ctccccgacc cggccggtcc cgacgcgcag gccgcgacgg tcggggagac cacggaggcc
      301 ctgcggttcc tgtatcgggc gctctccccc gtcaccgccg agctcccggt ggcggacgtg
      361 acccatgcca cctcatccgg tatggccgcg atccccggga tcctggccgc cgtggaacgc
      421 cgcacggcca tgatcgtgag cgagcccacg ctgtacctgc gcgaccagta cctcacgctg
      481 tcgcgccacg ggctgccctt cccgctgaag cggctgctgc tgcggttctc cagcgcgatg
      541 gcgcagaccg cgtatcactt cgcggaccgc gtggcgcccg catgcgaatg gggaggccgg
      601 tgggaacagg cctacggggt ccctcccgac cgcatcgagg tgatctaccc cggcgtcgac
      661 caggacgcgt tccgcccaat gccgatggac cggtccgagc gccccacggt cgtctccctc
      721 tctcccatgg agccccggga ggacctcgag accctgctgg acgtggccga ccgggtacgc
      781 cgggccgttc ccgacgtcct gttcatgcat cacgggccgg ccgtggacgg gccgtactcg
      841 gagcggattc gcgcgttggc ccgcgatcgc ctgctcgagg acaccgtcaa gttcatggga
      901 ccgacggacc gggtcgccgc cgcgctggcc tccgccgatc tcatggtgtc gaccacgacg
      961 tcggagacct tcccgctcgc cgtggtcgag tcgctggcct gcgggacccc cgtcgtggcg
     1021 acggcggtgg gcggggtcca cgaggccctc gagggccccg gcctgaccgc gccgcccggc
     1081 gacgccgagc gcctggccga ggcggtcgtg gccatcctgc ggctgcccga cgagcaacgg
     1141 cagctgctcg cggaggcggc ccggagcatc gccacgcaga agttcggtct gtcgacgttc
     1201 gtcgaacggt atcgggaagc ctatgtcgaa ctgtgggggg tcccggtcga ggccgtgccc
     1261 gccgctcgca aggaggaggt gcccatgggg gtcgcggtcg cgaagcgcga aatcgaggtg
     1321 caggcagagg ccgaggccga ggtgccagcc gccgcctcac cggtcccggc ggcggcccca
     1381 cgggtcccgg cagcggcccc acgggtcccc gcggcggcct tcgtgcgcac tcccgccgct
     1441 gccgccgcgg ccatccctcc tgctccgccg atgccgcgcg agcgacgcgc ggagcccgcc
     1501 ccatccgtcg ctccgcccac gccggcgcgc cggctcgtcc cggcggccgc cgcgacgccc
     1561 gggcggaggc cgctgctgtg ggaggtcccc gcccccgcgc ctgcgggcgc cgctgcgttc
     1621 tcgggtcccc tgcgcgctgg ttccgcggac cgggccgtga cgccgacacc gaccccgacg
     1681 ccctctcggc cggcgccgac gccggcccgg ccgaacacgc cgtccgtccg caagctgtcc
     1741 acgtcccgac ccccagcgga acccacggcg gctccggccg ccccccctcg cctggttccc
     1801 gtgccgaacg gcatggcgcc cagcgagctc gggctggccc tcgagagcga ggaccccttc
     1861 gcccgggtcg gcgccctcag cagggccggc ggcgaaccgg gactggccga cgtcgtcgcg
     1921 acggcgctgg ccgacgacta cccgcaggtt cggagggaag ctgttcgcgc cctccagagg
     1981 atcggcggcc cgctggccgg ccgggccctc gccgacgccg tcgccc
//
LOCUS       DASPWO010000423         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_402703, whole genome shotgun sequence.
ACCESSION   DASPWO010000423 DASPWO010000000
VERSION     DASPWO010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402703"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1963)
                     /locus_tag="VEO00_09640"
     CDS             complement(<1..1963)
                     /locus_tag="VEO00_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58297.1"
                     /translation="MATITHRHDPEQCPSCDLGAFSRNNFFTGKLLVERDFTDEQTYV
                     SDKIRHHNRRLHGWGVVCGLRVKQHPSSDCRDRFVCIEPGTALDCCGHEIVARDEGCF
                     DLTQLPAVQALGAPPEGGSGGTDDAAHTLQICLRYRECGTEPIPVLYDECGCDDTRCL
                     PNRILESFEVDVQVDPPAAAETWTGPQIERGTDIGLAGATRVAFNPADGLLYVVAGTT
                     LHAVDRSSGAILRSHDLGSTVHGLDVSPGGTHLYAVRDTDGGGLTLTVLQASDFSDTF
                     EDDLPNGQAPVATAVSPAADGRFLVLVTADKKLQVYGPDLEGGAPAAPTAIDVAADRT
                     LLAIARNGATAYVAGTTVGSPTDPAPLEAMDLGGGPGDPALGALLPGVEPSALSTVAD
                     GTAQLLVVGDANGGASVVDPAASTVSGPVALAGPATELAGDLWVYALQTAGGASALQA
                     VGVPRVVRGEPDPVGPAVAFAGVAHDLAVAPGAIYVAYAASGGDPGGVAVFGVLERDC
                     RDILWGSLDGCGDCEEPNCVVLATISGYRPGHAILDLADPPTAPADDDAAGIARIDNR
                     AGRRLLPSTSVLTDLITCLLDEGVGGGGPGPQGPAGPQGPQGEQGPQGVQGLQGVQGP
                     QGLPGSQGLQGPEGPAGPPGPGLEPGLVQI"
BASE COUNT          256 a          748 c          758 g          265 t
ORIGIN      
        1 tgatctgaac gagcccaggc tcgagacccg gccccggcgg gccggccggg ccctcggggc
       61 cctgcagccc ctgtgacccc ggcaggccct gcggaccctg cacgccctgc aacccctgga
      121 cgccctgggg gccctgctcc ccctggggtc cctgggggcc cgcgggtccc tggggccccg
      181 gtcccccacc gccgaccccc tcgtccagga gacaggtgat caggtcggtg agcaccgacg
      241 tgctggggag caaccgccgg ccggcgcggt tgtcgatgcg cgcgatcccc gccgcgtcgt
      301 cgtcggccgg cgccgtcggg gggtccgcga ggtcgaggat ggcgtggccg gggcggtagc
      361 cgctgatcgt cgcgagcacg acgcagttcg gctcctcgca gtcgccacag ccatcgaggg
      421 atccccacag gatgtcccgg cagtcccgct ccaggacgcc gaacacggcc acccctccgg
      481 ggtccccgcc cgacgccgcg tacgcgacgt agatcgcgcc gggcgcgacg gccaggtcgt
      541 gcgcgacgcc ggcgaacgcg accgccgggc cgaccgggtc cggctcgccg cgcaccacgc
      601 gcgggacgcc gacggcctgg agggcgctcg cgccgcccgc cgtctgcagc gcgtagaccc
      661 acagatcccc ggccagctcc gtcgccgggc cggcgagcgc caccgggccg gacacggtcg
      721 acgcggcggg gtcgaccacg gaggcgccgc cgttcgcgtc ccccaccacc agcagctggg
      781 ccgtcccgtc ggcgacggtg gacagcgcgc tcggctcgac gcccggcagg agcgcaccga
      841 gcgccgggtc acccgggccc ccgccgaggt ccatggcctc gagcggcgca gggtccgtcg
      901 gcgatccaac cgtcgtacct gccacgtacg ccgtcgcgcc gttccgtgcg atcgcgagca
      961 gcgttcgatc cgcggccacg tcgatcgcag tgggcgcggc cggcgcgccg ccctcgaggt
     1021 cggggccgta gacctggagc ttcttgtcgg ccgtgacgag cacgaggaag cggccgtccg
     1081 ccgcgggcga gaccgccgtg gcgaccggcg cctggccgtt cggcaggtcg tcctcgaacg
     1141 tgtccgagaa gtcgctcgcc tgcaggaccg tgagcgtgag ccccccgccg tccgtgtccc
     1201 gcaccgcgta caggtgggtc ccgccgggcg agacgtcgag gccgtgcacc gtggacccca
     1261 ggtcgtggga acggaggatc gcgcccgacg accggtccac cgcgtggagc gtggtgcccg
     1321 ccaccacgta gaggaggccg tccgcggggt tgaacgcgac ccgtgtcgcg ccggcgaggc
     1381 cgatgtcggt gccgcgttcg atctgggggc cggtccacgt ctcggctgcg gccggggggt
     1441 cgacctgcac gtcgacctcg aacgactcga ggatccggtt ggggaggcac cgggtgtcgt
     1501 cgcacccgca ttcgtcgtac agcacgggga tcggctcggt gccgcactcg cgatagcgga
     1561 ggcagatctg gagcgtgtgc gccgcatcgt cggtcccgcc ggagcctccc tcgggaggag
     1621 ctcccagcgc ctggaccgct gggagctggg tcaggtcgaa gcacccctcg tcccgcgcga
     1681 cgatctcgtg gccacagcag tcgagggccg tcccgggctc gatgcacacg aaccggtctc
     1741 gacagtcgga gctcgggtgc tgcttgacgc ggagcccgca gacgacgccc catccgtgca
     1801 gccggcggtt gtggtgacgg atcttgtcgc tgacgtacgt ctgctcgtcg gtgaagtcgc
     1861 gttccacgag cagcttgccg gtgaagaagt tgttgcggga gaaggcgccg aggtcgcacg
     1921 aggggcactg ctcgggatcg tggcggtgcg tgatggtcgc catggaatcg tctccctcag
     1981 tccagtacgg cggtcgtccc gatgcggctg gcgtcgacgg ccgggga
//
LOCUS       DASPWO010000424         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_62475, whole genome shotgun sequence.
ACCESSION   DASPWO010000424 DASPWO010000000
VERSION     DASPWO010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62475"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VEO00_09645"
     CDS             <1..242
                     /locus_tag="VEO00_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HYU58298.1"
                     /translation="LTAKGKISVPAAVRRRLGIGPGSVLEWLDEGERVVVRRVGRHSS
                     EDIHHALFGTRPRRRTLLQLKAGVKAYIRRRRTRR"
     gene            complement(259..1251)
                     /locus_tag="VEO00_09650"
     CDS             complement(259..1251)
                     /locus_tag="VEO00_09650"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018254905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HYU58299.1"
                     /translation="MTARVLPADDPAAVAEAARLLKAGGVVAFPTETVYGLGADAFNP
                     AAIARVFEVKARPSFDPLIVHVARAEEVGRVARGKGTGDVDPRVALLAERFWPGPLTL
                     ILARQRALPDLVTAGLDTVGVRVPAHPAARDLIRATGTPVAAPSANPFGYVSPTSADH
                     VVAQLGEKIDLVLDGGPCTVGLESTILSLAADPPLILRPGAVTREQLEAALGTSLEVA
                     GPTERPRAPGQLPRHYATRTPLRILDRPAGPAPKGVGRVGLLAIIGSPRASGYAAVEI
                     LSPDGTLASAAANLFAALRRLDGAGLDLVLAEPCPEDGLGCAIMDRLRRCASPR"
     gene            complement(1248..2309)
                     /locus_tag="VEO00_09655"
     CDS             complement(1248..2309)
                     /locus_tag="VEO00_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYU58300.1"
                     /translation="MHLRSHTPESLVTQWPDLGLDLARARRVVQRLVGDDRDDLEGVR
                     GLSKGTAREILARGRTDRLAVVDRRRSEVDPFVKYLFRAGDGVVFEAVRIPLERPRWS
                     VCVSSQVGCALGCVFCETGRVGFTRNLEPWEIVEQVLTVRRESPERPVTGVVFQGQGE
                     PFQNYENVLRAAAILRDPCGARIRGDRITISTVGLVPQIERYTNEGHPYRLILSLTSA
                     FSEKRAALMPITARHDVPELAAAMRRHAARRGGPVHLAWVLMAGVNTGADEAGELARL
                     FAGVPVRVSVIDVNDPTGRFLRASDEERSRFFSALAENGLGFVRRYSGGPDIHAACGM
                     LASRQQGGRTIGSGAPEAV"
     gene            complement(2312..>2679)
                     /locus_tag="VEO00_09660"
     CDS             complement(2312..>2679)
                     /locus_tag="VEO00_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58301.1"
                     /translation="TPPGPSPTAAPPLPDPGPAMPSAMVVPPEQVDTRPVAAPPPASP
                     VSLLAPPKAEAGGPDQPAGAPPELRLNAISVRDGHPVAIVNDRLVREGDSFDGIRILK
                     IGDAEVEVEVAGQRRTLRF"
BASE COUNT          373 a          912 c         1051 g          343 t
ORIGIN      
        1 ggctcacggc gaagggcaag atctcggttc ccgccgcggt tcgtcggcgg ctcggcatcg
       61 ggcctggatc cgtcctcgag tggctggatg agggcgagcg ggtcgtggtt cgacgcgtcg
      121 gtcgccacag ttccgaggac attcaccatg ctctgttcgg gacgcggccg cggaggcgga
      181 cgcttcttca gctgaaggcc ggcgtgaagg catacatcag gcggcggcgt acccgccgtt
      241 gaggcggtcg accgcgcttc accgcggcga cgcgcacctc cggaggcggt ccatgatcgc
      301 gcagcctagg ccgtcttccg ggcagggctc ggccaggacg aggtccaggc cggcgccgtc
      361 gaggcggcgc agggccgcga acaggttggc ggcggcgctg gccagcgtcc catccgggga
      421 gaggatctcg accgcggcgt agccggaggc gcgcggggag ccgatgatgg cgaggaggcc
      481 cacgcggccg acgcctttgg gcgccggacc cgcggggcgg tcgaggatgc gcagcggggt
      541 gcgcgtcgcg tagtgccggg ggagctgtcc cggcgcgcgc ggccgctcgg tcggccccgc
      601 cacctcgaga gacgttccca gcgccgcttc gagctgctcg cgggtgaccg cgcccggccg
      661 caggatcagt gggggatcgg ccgccaggga gaggatcgtg gactcgaggc ccaccgtgca
      721 gggtccgccg tcgaggacga ggtcgatctt ttcgccgagc tgggcgacca cgtggtcggc
      781 gctcgtaggg ctcacgtagc cgaacgggtt ggcactcggg gcggcgacgg gcgtgcccgt
      841 agcgcggatc agatcgcggg ccgcgggatg agcggggacc cgcacgccga ccgtgtcgag
      901 gccggccgtc accaggtcgg gcagagcccg ctgccgtgcc aggatgaggg tgaggggccc
      961 cggccagaag cgctcggcga gcagggccac gcgcggatcc acgtccccgg tgcccttccc
     1021 gcgggcgacg cggccaacct cctccgcacg cgcgacgtgg acgatgaggg gatcgaagga
     1081 cgggcgggcc ttgacctcga acacgcgggc gatcgccgcc gggttgaacg catcggcgcc
     1141 caggccgtag acggtctcgg tggggaaggc tacgacgccg cccgctttca gcaggcgcgc
     1201 ggcttcggca acggcggccg gatcgtcggc cgggaggacc cgagccgtca gaccgcctcc
     1261 ggggccccgg agccgatcgt ccggccaccc tgctgccgcg aagcgagcat gccacaggcg
     1321 gcgtggatgt cggggccgcc ggagtagcgc cgcacgaagc ccaggccgtt ctccgccagg
     1381 gccgagaaga agcgcgaacg ctcttcgtcg gatgcgcgca ggaatcggcc cgtcggatcg
     1441 ttcacgtcga tcaccgacac ccggaccggc actcccgcga acaggcgggc gagctcgccg
     1501 gcctcgtcgg cgccggtgtt gacgcccgcc atgaggaccc aggccaggtg gacgggtccc
     1561 ccgcgccgcg cggcgtggcg gcgcatggcc gcggccagct ccggaacgtc atggcgggcc
     1621 gtgatcggca tgagggcggc gcgtttctcg gagaaggccg aggtgaggga caggatcagc
     1681 cggtaggggt ggccctcgtt ggtgtagcgc tcgatctgcg ggacgaggcc cacggtcgag
     1741 atcgtgatcc ggtcgccgcg aatgcgggcg ccgcacgggt cgcgcaggat cgcggcggcg
     1801 cggaggacgt tctcgtagtt ctggaagggc tcgccctgtc cctggaagac gacccccgtg
     1861 accggccgct cgggggactc gcggcgtacg gtcaggacct gctcgacgat ctcccacggc
     1921 tccaggttcc gggtgaagcc cacccggccg gtctcgcaga acacgcagcc gagggcgcag
     1981 cccacctgcg acgacacgca gacgctccag cggggacgct ccagggggat gcgcacggcc
     2041 tcgaagacca cgccgtcgcc ggcgcggaac aggtacttca cgaatgggtc gacctcgctg
     2101 cggcggcggt cgaccaccgc gagccgatcg gtcctccccc gcgccaggat ctcccgtgcc
     2161 gttcccttcg agaggcctcg gactccctcc aagtcatcgc ggtcgtcgcc cacgagccgc
     2221 tggaccactc ggcgagcccg ggccaggtcg aggccgagat cgggccactg ggtgaccagg
     2281 ctctcggggg tgtggcttcg aaggtgcatg gctagaagcg cagcgtccgg cgctggccgg
     2341 ccacttccac ctcgacctcg gcgtcgccga tcttcaggat ccggataccg tcgaagctgt
     2401 cgccttcgcg gacgaggcga tcgttcacaa tggccaccgg gtggccgtcg cgaacgctga
     2461 tcgcgttgag gcggagctcc ggcggtgcgc ccgcgggttg atcaggcccg ccggcctcgg
     2521 ccttcggcgg tgcgaggagg gagacgggag acgccggggg tggggcggcg accgggcgcg
     2581 tgtcgacctg ctcgggggga accaccatgg cggagggcat cgctggcccc gggtccggca
     2641 gcggcggagc cgccgtcggc gaagggcccg gaggggtcg
//
LOCUS       DASPWO010000425         4718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_402761, whole genome shotgun sequence.
ACCESSION   DASPWO010000425 DASPWO010000000
VERSION     DASPWO010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402761"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VEO00_09665"
     CDS             complement(<1..1119)
                     /locus_tag="VEO00_09665"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HYU58302.1"
                     /translation="MRIVVVGGAGAMGRVAVDDLAADPGVALVTVADADGVRAREVAE
                     AAAGQGGKVRDIEADVRDPAFPGLLAGHHVCLNAASYRLNLTVMEASLAAGCHYLDLG
                     GLFHVTRRSLELSPRFEAAGLTGVVGVGGGPGMTNLMAVLGARELGAVRAVHVRMGSR
                     DPSVAGSPLPVPYSLDTILDEFSHPAMAWRDGDFVEVPPLGEPEDVDFPPPVGRMTAV
                     TTLHSEVATLPAHFADRGIREVTFKIAFDPAFVERFRLLAAIGLAGEDPVEVAGVSGG
                     TVRPRDVLAALARRFPPAAGTDDHEALRVVLEGERDGAPSRVVVDCVVDPDPARGMGA
                     GARDTGIPPSIVAQMLARGEIDRPGVHAPEDVVPLDAFL"
     gene            1211..2752
                     /locus_tag="VEO00_09670"
     CDS             1211..2752
                     /locus_tag="VEO00_09670"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HYU58303.1"
                     /translation="MAVAVADIVRNPALGLSLVAGRAGARAPIRWVHVSELEDPTPFL
                     RGGELLLTTGMGLGETPARQRAYVARLVKAGLSGLGFGTGFGFPTVPRAVASECERHG
                     FPVFEVPYEIPFIAITEAVFTRLVAEQYEELQRSVEAQQVLTRAVLEGGGIPGLVRTL
                     AALVKGWAVVLDLHGLPLAAAPAAAEVRAPRLWEELRESRPEGTGFSLSLADLGHHVA
                     VQPVGAGHVEAFLAVGKRKALTHVDRLVAAHALSLLAIELDKARAVAEAERALRGDLL
                     EALWQGALGPEEVERGLRRFGFRPGSSVRGIAVAPDGAPAGDVEWALADALSRRRGGF
                     LTSVREDTVFALIEGDAGPVPADLRRDVARRSGADVTVATGSDAPAAEAGRSIREATY
                     ALRVCRVEGRREAGFAELGSYRLLLSMQDPDALRAFADSVLGPLEAYDLDHHGDLVAS
                     LEVFLERNAKWEHAAADLFVHRHTLRYRMHKVEELTGRDLALPHDRIEFWLALRAREL
                     LATLR"
     gene            2876..4321
                     /locus_tag="VEO00_09675"
     CDS             2876..4321
                     /locus_tag="VEO00_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HYU58304.1"
                     /translation="MAVTAEEIARKGMFIGGEWTAGSGEAEPIHDPATGQVLAHVAMG
                     TEADVDRAVAAARTAFESWQDVTPRERAGMLLKLADAIEEHADELWALESANVGKPSA
                     WRDDEMGPCSDNLRFFAGAARTMTGQVAGEYMAGYTSMLRREPVGVVGSIAPWNYPLM
                     MGVWKVGPALAAGNTVILKPSEWTPLTALRLAEIAAEIFPPGVLNVLTGHGEPVGAGL
                     VRHPGVGMVSLTGDVATGKAVARAASETLKRVHLELGGKAPVLVFDDADPKAVAEGVK
                     VGGFFNSGQDCTAASRVVAGPKVYDGLLAELSDAVESLVVGDPSDGDTEMGPLVSKEQ
                     LERVTGFVDRAVGAGATVVTGGSTMDGGGYFYRPSVITDVAQDSEIVQREVFGPVVTV
                     QRFADDDEAIAWANDVDYGLAASVWTRDVGRALNAARRLKFGTVWINDHIPLVSEMPH
                     GGYKQSGYGKDLSVYALEDYTIIKHVMAKID"
     gene            4386..>4718
                     /locus_tag="VEO00_09680"
     CDS             4386..>4718
                     /locus_tag="VEO00_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU58305.1"
                     /translation="MSEIDVRLRGVTKRFGDTVAVDDMNLDVERGEFFSLLGPSGCGK
                     TTTLRMIGGFEDPTEGAIYLQGHDVTGLPPYRRSVNTVFQSYALFPHLSVFENVAFGL
                     RRKRASRSE"
BASE COUNT          639 a         1643 c         1817 g          619 t
ORIGIN      
        1 gaggaacgcg tcgagcggaa cgacgtcctc gggcgcgtgg acgccgggcc ggtcgatctc
       61 gccgcgggcg agcatctgag ccacgatcga gggcgggatg ccggtgtcgc gcgctccggc
      121 gcccatcccg cgcgcggggt cggggtcgac cacgcagtcc accaccaccc gcgagggggc
      181 gccgtcgcgt tcgccctcca ggacgacgcg cagcgcctcg tggtcatcgg tcccggcggc
      241 gggtgggaag cgacgggcca gcgcggcgag cacgtcgcgc ggacgcaccg tcccgccgga
      301 gacgcccgcc acctccaccg ggtcctcgcc ggccaggccg atcgccgcga gcaggcggaa
      361 gcgctcgacg aatgcagggt cgaaggcgat cttgaacgtg acctcgcgga tcccacgatc
      421 ggcgaagtgc gccggaaggg tggccacctc cgagtgcagc gtggtcaccg ccgtcatccg
      481 cccgaccggc ggcgggaagt ccacgtcctc gggctcgccg agcggcggga cctcgacgaa
      541 gtcgccgtcg cgccaggcca tcgccgggtg gctgaactcg tcgaggatgg tgtccagcga
      601 gtaggggacg ggaagcgggg agccggcgac gctcgggtcg cgcgacccca tgcggacgtg
      661 gacggcgcgc accgcgccga gctcgcgcgc cccgagcacc gccatcaggt tcgtcatccc
      721 cgggccgccg ccgacgccca caacgcccgt cagcccggcg gcctcgaagc gcggcgacag
      781 ctcgagcgac cgccgggtca cgtggaacag cccgccgagg tcgaggtagt gacagccggc
      841 ggcgagcgac gcctccatca cggtcaggtt cagccggtag gaggcggcgt tcaggcagac
      901 gtggtgaccg gcgaggaggc cggggaacgc cgggtcacgg acgtcggcct cgatgtcgcg
      961 gaccttcccg ccctgcccgg ccgccgcctc ggccacctcc ctggcccgca cgccgtcggc
     1021 gtcggcgacc gtgaccagcg ccacgccggg atcggccgcc aggtcgtcca ccgcgacgcg
     1081 gcccatggcg ccggcgccgc ccacgaccac gatgcgcatg ccctcagtct ccggctcgcg
     1141 gcgcggtccc ggaaggtaca ggccggacac cgccacggcg ggccgtttgt acgctgcgta
     1201 caatcgcgcc gtggccgtcg ccgtcgccga catcgtgcgc aatcctgcgc tcgggctgtc
     1261 gctcgtcgcg gggagggccg gggcgcgggc gccgatccga tgggtccacg tcagcgagct
     1321 cgaggacccc acgccgttcc tgcggggcgg cgagctgctg ctgacgaccg gtatgggcct
     1381 cggcgagaca ccggcccggc agcgcgccta cgtcgcccgc ctggtgaagg ccggactctc
     1441 cgggctgggg ttcggcaccg ggttcggctt cccgacggtg ccgcgcgcgg tcgccagcga
     1501 gtgcgagcgc cacggtttcc cggtgttcga ggtgccgtac gagatcccgt tcatcgcgat
     1561 caccgaggcg gtcttcacgc ggctcgtcgc ggagcagtac gaggagctgc agcggtcggt
     1621 ggaagcgcag caggtgctga cccgagccgt cctggagggc ggcgggatcc cggggctggt
     1681 gcgcaccctg gccgcgctgg tgaagggctg ggccgtggtc ctcgacctcc acggtctccc
     1741 gctggccgcc gcgccggcgg cggccgaggt gcgcgccccc cggctgtggg aggagctgcg
     1801 cgagtcccgc cccgagggga ccgggttcag cctgtcgctc gccgatctgg gacaccacgt
     1861 cgccgtgcag ccggtcgggg ccggccacgt cgaggcgttc ctcgcggtcg ggaagcggaa
     1921 ggcgctgacc cacgtggacc gcctggtcgc ggcccacgcc ctctcgctcc tggccatcga
     1981 gctggacaag gcgcgtgccg tggcggaggc ggagcgcgcg ctgcgcggtg acctgctcga
     2041 ggccctgtgg cagggggcgc tcggtccgga ggaggtcgag cgtggcctgc gccgcttcgg
     2101 cttccggccg ggatcgagcg tgcgggggat cgccgtcgcg cccgacggcg cgccggccgg
     2161 cgacgtggag tgggcgctcg ccgacgcgct ctcccggcgc cgcggcggat tcctcacctc
     2221 cgtgcgcgag gacacggtct tcgcgctgat cgaaggggac gccggtccgg tgcccgccga
     2281 tctgcgccgc gatgtggcac ggcgctcggg cgccgacgtc accgtcgcca ccgggagcga
     2341 cgcgcccgcg gcggaggccg gccgatcgat ccgcgaggcc acctacgcgc tgcgcgtgtg
     2401 ccgcgtcgag gggcggcggg aggcgggctt cgccgagctg ggctcgtatc ggctgctgct
     2461 ctcgatgcag gacccggacg cgctgcgcgc cttcgccgac agcgtgctcg gcccactcga
     2521 ggcctacgac ctcgaccacc acggcgacct ggtcgcctcg ctcgaggtgt tcctggagcg
     2581 caacgcgaag tgggagcacg ccgcagccga cctgttcgtg caccgccaca ccctgcgcta
     2641 ccggatgcac aaggtggagg agctcaccgg ccgcgacctc gccttgcccc atgaccggat
     2701 cgagttctgg ctggcgctcc gagcccggga gctgctggcg acgctccgat agtgctggtg
     2761 gcgcgtaccg gttcgtacaa acggcgggtc ctgaatagcc gcttcgtaga cagcccgccg
     2821 agagggccga tggtacgttc cgccgggagg gcgcggctgc gggggtgatg ggacgatggc
     2881 ggtgacggcg gaggagatcg cccgcaaggg catgttcatc ggcggggagt ggacggccgg
     2941 gtccggcgag gccgagccga tccacgaccc cgcgaccggc caggtcctgg cgcacgtggc
     3001 gatggggacc gaggccgacg tggaccgggc cgtagccgcg gcgcggacgg cgttcgagtc
     3061 ctggcaggac gtcacgccgc gagaacgcgc cgggatgctc ctgaagctcg ccgacgcgat
     3121 cgaggagcac gccgacgagc tgtgggcgct cgagtccgcg aacgtcggca agccgtcggc
     3181 gtggcgcgac gacgagatgg ggccgtgctc cgacaacctg cgcttcttcg ccggggcggc
     3241 ccggacgatg accggccagg tcgccgggga gtacatggcc ggctacacga gcatgctgcg
     3301 ccgcgagccg gtcggcgtgg tcggctcgat cgccccgtgg aactacccgc tgatgatggg
     3361 ggtgtggaag gtggggccgg cactcgcggc cgggaacacg gtgatcctga agccgtcgga
     3421 gtggacgcca ctgaccgcgc tgcggctcgc cgagatcgcc gccgagatct tccctcccgg
     3481 cgtgctgaac gtgctgaccg ggcacggcga gccggtcggg gccgggctcg tccgccatcc
     3541 gggcgtgggg atggtctcgc tgaccggcga cgtggcgacc gggaaggcgg tcgcgcgcgc
     3601 agcgagcgag acgctgaagc gcgtgcacct cgagctgggc gggaaggcgc cggtgctcgt
     3661 gttcgacgac gccgacccga aggccgtcgc cgagggggtg aaggtcggcg ggttcttcaa
     3721 ctccggccag gactgcacgg cggcgtcccg cgtcgtggcc gggccgaagg tctacgacgg
     3781 cctcctcgcg gagctgtccg acgcggtgga gtcgctcgtg gtgggcgatc cgagcgacgg
     3841 ggacaccgag atggggccgt tggtgtcgaa ggagcagctc gagcgcgtga cggggttcgt
     3901 cgaccgggcc gtcggcgccg gcgccaccgt cgtcaccggg gggagcacga tggacggcgg
     3961 cggctacttc taccgtccca gcgtgatcac cgacgtggcc caggactcgg agatcgtcca
     4021 gcgcgaggtg ttcggcccgg tggtgacggt gcagcgcttc gcggacgacg acgaggcgat
     4081 cgcctgggcg aatgacgtcg actacgggct cgccgcctcg gtctggaccc gcgacgtcgg
     4141 gcgggccctg aacgccgcgc ggcggctgaa gttcgggacc gtctggatca acgaccacat
     4201 cccgctggtc tcggagatgc cacacggggg ctacaagcag tccgggtacg gcaaggacct
     4261 ctccgtgtac gcgctggagg actacacgat catcaagcac gtcatggcga agatcgactg
     4321 atgagcgagc ctcggacctc cccgaccggt ggggggtggg gagggtctcc tccccacaca
     4381 gccgaatgag cgagatcgac gttcggctgc gcggggtgac caagcgcttc ggggacacgg
     4441 tcgccgtcga cgacatgaac ctcgacgtcg agcgcggcga gttcttctca ctgctggggc
     4501 cctcggggtg cgggaagacc acgacgctgc ggatgatcgg cggcttcgag gacccgaccg
     4561 agggcgcgat ctaccttcaa ggccacgacg tcaccggcct gccgccgtac cggcgcagcg
     4621 tcaacacggt gttccagtcc tacgcgctgt tcccgcacct ctcggtgttc gagaacgtcg
     4681 ccttcgggct gcgccggaag cgggcgtcgc gctcggag
//
LOCUS       DASPWO010000426         4858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_992409, whole genome shotgun sequence.
ACCESSION   DASPWO010000426 DASPWO010000000
VERSION     DASPWO010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992409"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VEO00_09685"
     CDS             <1..186
                     /locus_tag="VEO00_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018728702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58306.1"
                     /translation="KQKAGKARMRKVGRVDVPQEAFIAALRVERGGGGSGGIARPGER
                     GARPRPASGAPGGKGRP"
     gene            183..1286
                     /gene="hemW"
                     /locus_tag="VEO00_09690"
     CDS             183..1286
                     /gene="hemW"
                     /locus_tag="VEO00_09690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HYU58307.1"
                     /translation="MTAAGLYVHVPFCLTRCGYCDFNVVVGREREAGRYLRAVAAEAS
                     REAPAWRGTPFASIYVGGGTPTLVPAGELVQLLDRLRKAFAVAPGAEVTVEANPETVD
                     ERSLRTLRAGGVNRLSLGAQSFDPAVLAALDRAHGPDTVREAVRAARRGGLENVSLDL
                     IYGAGPEDGPSWARTLRAALDLDVEHVSCYALTVEPNTELASRVRGGSVPAPDADEQA
                     DRYEAACRVLGGAGFDHYEVSNWARPGRRSVHNGGYWEGRPYLGLGAGAHSFAAARRR
                     WNVRPLGRYVAAVEAGRSPVGGEERPDTTARRTERLLLGLRTAAGLPLAGLDPERIRA
                     LAEARLLRLDGDRAVPTEPGMLLANELVLELSG"
     gene            1340..2473
                     /gene="hrcA"
                     /locus_tag="VEO00_09695"
     CDS             1340..2473
                     /gene="hrcA"
                     /locus_tag="VEO00_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HYU58308.1"
                     /translation="MTSIHPSARGARGRREDRPAGESRPRHRPPARGSRPDLDERKAR
                     ILRAVVVTHIESAEPVGSGQVARRFRLGVSSATIRSEMSALEDLGFLVQPHTSAGRVP
                     TDLGYRFYVDTLPEPSRLPDGVREAIRRFFASAPDMEETIRGTTRLLSTLTHHAALAL
                     APSIRESRISVLDLVGTGSVWLLVVVTETGAVEKRVLEPGAVSGRAAERLRRDLNEGF
                     RGLTLAGAAARADALAGAAAEAARDEERRLLEAVAAALHPVRRDERVIVGGAANIVGE
                     EGFDRRDTIEGVIRALEEEAQFLTMLRAAATSGDLTVTIGRENPVTAMREASIVIAPY
                     GPDDRTLGTVGVVGPTRMDYQSAIAAVQAVAERLSRVLQALAR"
     gene            2590..3705
                     /gene="dnaJ"
                     /locus_tag="VEO00_09700"
     CDS             2590..3705
                     /gene="dnaJ"
                     /locus_tag="VEO00_09700"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HYU58309.1"
                     /translation="MARQEIPDLYAVLGVSPDATEEEITRAYRRLAREHHPDVNDDPT
                     AEQRFKEIAAAYETLSDPAKRRQYDRFGAGGVSLGDPFPFGDLTDLFDAFFGGGFRRP
                     RGPRTRARRGEDLGVQVDLSFEEAAFGVEKEVAVQSRERCPRCTGTGCEPGTHPSRCS
                     RCGGAGEIQDVARSVFGTVMTARPCPRCEGTGREIATPCADCGGEGRIPKRERVTVEI
                     PAGVADGTELRVGGAGAAGRNEGPPGDLFVTVRLRPHAVFERRGQDLVAALRITFTQA
                     ALGADLDVSTLDGTEKVRIDPGTEPGTVIRLRGRGVPNLAGRGRGDLYLTVQVEIPKG
                     LLKRERELLHELAAIRGEEPEVRGKRRTGLLGRLIGP"
     gene            4206..4433
                     /locus_tag="VEO00_09705"
     CDS             4206..4433
                     /locus_tag="VEO00_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HYU58310.1"
                     /translation="MLYERAERPFSACLPEDPAIVGLHVGPEGGLGERDLAEAAAASA
                     VLVSLGAPNLRTETAAVVVAALALNRYGRLG"
     gene            4490..4834
                     /locus_tag="VEO00_09710"
     CDS             4490..4834
                     /locus_tag="VEO00_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HYU58311.1"
                     /translation="MVDCFFCQIAAREQPADIVHSTEHVVAFRDINPQAPTHLLIISR
                     EHIESARALRDRHAAVLAEIFQAAGHLARAEGVDSSGWRLVTNVGPDAGQSVHHLHFH
                     LLGGRHMGWPPG"
BASE COUNT          615 a         1739 c         1894 g          610 t
ORIGIN      
        1 aagcagaagg ccggcaaggc acggatgcgc aaggtcggcc gggtggacgt gccgcaggag
       61 gccttcatcg cggcgctgcg cgtcgagcgg gggggcgggg ggtccggggg gatagctcgc
      121 cctggggagc gcggagctcg accacgtccg gcgagcggag ctcccggggg gaaggggaga
      181 ccgtgacggc ggcgggcctg tacgtccacg tgccgttctg tctgacccgc tgcgggtact
      241 gcgacttcaa cgtcgtcgtc ggccgcgagc gcgaggccgg ccgctacctg cgcgcggtcg
      301 cggcagaggc gagtcgcgaa gcgccggcct ggcgcgggac gccgttcgcg tcgatctacg
      361 tcggcggagg gacccccacg ctcgtccccg ccggcgaact ggtacagctc cttgaccgtc
      421 ttcggaaggc gttcgccgtc gcgcccggcg ccgaggtgac cgttgaggcg aacccggaga
      481 ccgtcgacga acggtccctg cgaacgctcc gggcgggcgg ggtgaaccgg ctgtcgctcg
      541 gggcgcagtc gttcgacccg gcggtgctcg cggcgctcga ccgggcacac ggcccggaca
      601 cggtccgcga ggccgtccgg gcggcgcgcc gcggtggcct cgagaacgtg tcgctcgacc
      661 tgatctacgg cgccggtccg gaggacggcc cgtcgtgggc gcggacgctt cgggccgccc
      721 tggacctcga tgtcgagcac gtctcgtgct acgcgctgac cgtcgagccg aacaccgagc
      781 tcgccagccg cgtgcgcggc ggatccgtcc cggcgccgga cgccgacgaa caggccgacc
      841 gctacgaggc agcctgtcgc gtcctgggtg gggcgggctt cgaccactat gaggtctcga
      901 actgggcccg gccggggcgc cgttcggtgc acaacggagg gtactgggag gggcggccgt
      961 acctcgggct gggcgccggc gcccactcgt tcgccgccgc caggcgccgc tggaacgtcc
     1021 gcccactcgg tcggtacgtc gccgccgtgg aggccgggcg ctccccggtg ggaggcgagg
     1081 aacgtcccga caccacggcc cgccggaccg agcggctgct gctcggcctg cggacggccg
     1141 cgggtctgcc gctcgccggg ctcgaccccg agcggatccg cgccctcgcc gaggcgcgcc
     1201 tcctgcgcct ggacggcgat cgggccgtgc ccacggagcc cggcatgctc ctggcgaacg
     1261 agctcgtgct cgagctttcc ggataggccg cgcggtcacg ctgctagcac tcgcggtccg
     1321 agagtgctaa acagggaaag tgacgagcat ccatccgtcc gcgagggggg cccgggggcg
     1381 gcgcgaggac cgccctgccg gcgaatcgag accgcgccat cgcccgcccg ctcgtgggag
     1441 ccggcccgac ctggacgagc gcaaggcgcg gatcctgcgc gcggtggtgg tcacccacat
     1501 cgagagcgcg gagccggtcg gtagcggcca ggtcgcccgg cgcttccgcc tgggcgtcag
     1561 ctcggccacg atccggagcg agatgtcggc cctggaggac ctcggcttcc tggtccagcc
     1621 gcacacctcg gccggccggg tgccgaccga cctcggctac cgcttctacg tggacacgct
     1681 cccggagccg tcacgcctgc cggatggcgt gcgcgaggcg atccggcggt tcttcgcctc
     1741 ggcgcccgac atggaggaga cgatccgcgg cacgacccga ctgttgtcga cgctcactca
     1801 ccacgccgcg ctggcgctcg cgccgagcat ccgtgagagc cggatctcgg tgctcgatct
     1861 cgtcgggacc gggtcggtct ggctgttggt cgtcgtcacc gagacagggg ccgtcgagaa
     1921 gcgggtgctc gagcccggcg ccgtttccgg tcgggcggcc gagcggttgc ggcgcgacct
     1981 gaacgagggg ttccgcgggc tgaccctcgc cggcgcagca gcccgggcgg acgccctggc
     2041 cggggccgcc gccgaggcgg cccgggacga agagcggcgc ttgctcgagg cggtcgccgc
     2101 ggccctgcac cccgtccgcc gcgacgagcg ggtcatcgtc ggcggggcgg cgaacatcgt
     2161 gggcgaggag gggttcgacc gccgggacac gatcgagggc gtcatccggg ccctggagga
     2221 ggaggcgcag ttcctgacga tgctccgcgc cgccgcgacg agcggcgacc tgactgtgac
     2281 gatcggccgg gagaacccgg tcaccgcgat gcgcgaggcg agcatcgtca tcgccccgta
     2341 cgggccagac gaccgcacgc tcggcacggt cggcgtcgtc gggccgaccc ggatggacta
     2401 ccagtcggcg atcgccgccg tgcaggccgt cgccgagcgc ctctcgaggg tgctgcaagc
     2461 gctggcgcgg taggcgccac cgcacggcga acccgccgta tcctggaagg cttggggggg
     2521 tagctcgccc tgtggaggac ctcccccggc cacgtcctgc gagcgaactc cccgaggaga
     2581 acggaagcga tggcgcggca ggagattccc gacctctatg cggtcctcgg ggtgagtccc
     2641 gacgcgaccg aggaggagat cacccgcgcg taccggcggc tggcccgcga gcaccatccc
     2701 gacgtcaacg acgaccccac cgcggagcag cgcttcaagg agatcgccgc cgcctacgag
     2761 accctgtcgg acccggcgaa gcgccggcag tacgaccgct tcggtgccgg cggcgtcagc
     2821 ctcggggacc ccttcccgtt cggagacctg accgacctgt tcgacgcctt cttcgggggc
     2881 ggcttccgcc gtccgcgcgg cccgcggacg cgcgcccgcc gcggcgagga cctcggcgtc
     2941 caggtggacc tctcgttcga ggaagccgcc ttcggcgtcg agaaggaggt cgccgtccag
     3001 agccgcgaac ggtgcccgcg ctgtaccggc accggctgcg agccgggcac ccatccgtcg
     3061 cgctgctcgc ggtgcggcgg cgccggggag atccaggacg tcgcccgcag cgtgttcggg
     3121 accgtgatga ccgcgcggcc gtgcccacgg tgcgagggga cggggcgcga gatcgccacg
     3181 ccctgcgcgg actgcggggg cgagggccgg atcccgaagc gagagcgggt caccgtggag
     3241 atcccggccg gcgtggcgga cggcaccgag ctgcgcgttg gcggcgccgg tgccgccggg
     3301 cggaacgagg gcccgccggg cgacctgttc gtcacggtcc gcctgcgtcc gcacgcggtg
     3361 ttcgagcggc gcgggcagga cctggtggcc gcgctccgga tcacgttcac gcaggccgcg
     3421 ctcggcgccg acctcgatgt gagcacgctc gatgggaccg agaaggtgcg gatcgacccc
     3481 ggcaccgagc ccgggaccgt gatccgcctg cgcgggcgcg gcgtcccgaa cctcgccggg
     3541 cgcgggcggg gcgacctcta cctgaccgtc caggtggaga tcccgaaggg gctgttgaag
     3601 cgagagcgcg agctgctcca cgagctggcg gcgatccggg gagaggagcc cgaggtgcgc
     3661 gggaagcgcc gcaccggcct gctggggcgc ctgatcggtc cgtagcggcc gtgaaggggc
     3721 tgccatggtt ctacctgccc gagcgggtcc cggcggggac gcgggtcccg ctcgatccgg
     3781 atgacgcgcg tcacgccacc ggaccgctcc ggctgcgggc cggggtcgag ctgaccgtcg
     3841 gcgacggcgc cggctgggtc gccccggcgc ggctggtgga gtccgcccgc ggctcggcca
     3901 tggtcgacgt ggggacgggc ggatcgtcgc cccgtcgtcc ccgcgggtcc gcgtcgcggt
     3961 ggcggccccg gccggagatc gcgcctggtg gacggtgcag aagctcaccg agatcggcgc
     4021 cgaccgcatc gacgtggtcc gggccgagcg ctccggccgc cggccgggtg acgaggaaca
     4081 cctggcccgt cgcgcggcgg cggtggcgcg cgaggcggcg aagcagtcgc ggcgcccctt
     4141 catcccggag atcgggatcg ggcagggcct gcttcgccgg acgcgaggag ggggaggact
     4201 ccatcgtgct ctacgagcgg gcagagcggc cgttctcggc gtgcctgccg gaggacccgg
     4261 cgatcgtcgg gcttcacgtc gggcccgagg gcgggctggg ggagcgcgac ctcgccgagg
     4321 ccgccgcggc cagtgccgtc ctggtgtcgc tcggcgcgcc gaacctgcgc accgagaccg
     4381 cggccgtcgt cgtcgcggcg ctcgccctga atcgctacgg tcggctgggg tagggtgagg
     4441 cggcgtcgtc cgcggacgcc gagacctgag cggggagggc cgggtcggta tggtcgactg
     4501 cttcttctgc cagatcgccg cgcgcgagca gcccgccgac atcgtgcatt ccaccgagca
     4561 cgtcgtcgcg tttcgggaca tcaacccgca ggccccgacc catctgctga tcatctcccg
     4621 ggagcacatc gagtcggcgc gcgcgctccg cgaccgtcac gccgccgtcc tcgccgagat
     4681 cttccaggcc gccggccacc tcgcccgggc ggagggcgtg gactcgagcg gctggcgcct
     4741 ggtgacgaac gtcgggcccg acgcagggca gagcgtgcat cacctgcact tccacctgct
     4801 cggcggacgg cacatggggt ggccacccgg ctaggcgagc gggcctcctc ctgaccgg
//
LOCUS       DASPWO010000427         3930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_62869, whole genome shotgun sequence.
ACCESSION   DASPWO010000427 DASPWO010000000
VERSION     DASPWO010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62869"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..2957
                     /locus_tag="VEO00_09715"
     CDS             90..2957
                     /locus_tag="VEO00_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HYU58312.1"
                     /translation="MAEAAARLPRPRRRRRWLIIAIASAVALIALLGGLSGFYIDLLW
                     FREVGLSPVFASILWTKIVLVLLFGLVFFAVLYANLLIVRRTSPRYRLFSPEQEVIDR
                     YRQAADPYMRWILPGLSLLLALFAAASVAGLWREFLLWRNGAAVQFGVNDPVFQRDLS
                     FYVFRLPFLHFVQGWLFSSLVVITVVTAAAHYLWGGIRFQAIGEKVTPVVKAHLSVLL
                     GVIMLVKAWGYRLGQFDLLSSERGVVTGASYTDVNAQLPALRILVPIAILVAVLFLVN
                     IRFRGWALPILGIGLLALASIAAGAAYPAFVQKFRVDPQPLEQERPYIERNIEFTRRA
                     FGLDGVEPRRIDVQPDLTKGNVDDNPQTVSNIRLWEPALLQRAYQSLQQIRPYYSFED
                     VDVDRYTLPDGTRRVVMVSGREIAQSGIPEESANWQNRHLFFTHGYGYVASRVDRVST
                     EGQPDFLVRGIPPGGVGATSGDLAVGSPPGPLMYYGENEDVGFVVVKTRQKELDRPGG
                     QGVAEGTTTTYAGEGGIDLGGLFRRLAFAWHYRDVNLLISNLISPDSRILINRDIEDR
                     VRKAAPFLDLDHDPYMAVVDGRLAWIWDAYTTSDAYPYSTRLHPDDLVSTDGGPVRLP
                     GSVNYIRNSVKIAVDAYDGTMTFYIVDPEDPIVQVWQKAFPELFAPASAVSPGLRAHF
                     RYPEDLFTLQAEQYARYHVTDPDDFFSSEDFWAVPNDPNVADSRVKPYYVLMRLPGDT
                     EEEFVLFTPFTPRNRPNMIAWLAARSDPDAYGRITQIEFEGGQTIFGPEQVAARINQD
                     PTVSQQLTLLNQQGSTVQYGPLLAIPIEESFLYVQPLFLEASAGGGGAIPELQRVVVV
                     HGESVTIGDTLQETIAQSFGLEPQEPGGGEPPGQTIQELLDQASRHFARADRLLRQGD
                     LAGYQSEIDLARAAVEQALELAQGPDGGPPASPTPSPSATG"
     gene            2998..3071
                     /locus_tag="VEO00_09720"
     tRNA            2998..3071
                     /locus_tag="VEO00_09720"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3032..3034,aa:Met,seq:cat)
     gene            3384..>3930
                     /locus_tag="VEO00_09725"
     CDS             3384..>3930
                     /locus_tag="VEO00_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYU58313.1"
                     /translation="MYDTGVKDNHSLTVFWHHGTPNIGPPPEPLFPVSAELGIRWVSY
                     DRPGYGGSTRDPGRDIASAAAYVAAVADELSIDRFAVMGHSGGGPHALACGALLPARV
                     LGAVSMSGLAPYGAERLDWFAGMSAAGVASLRAAAAGRQAKEAYEASPPAGDLGFTDA
                     DEAALSGAWSWLIDVVRPAIAG"
BASE COUNT          566 a         1356 c         1367 g          641 t
ORIGIN      
        1 gacatcatcg acgggctggt cggcggccgc gggtaaacgg aactcgcgac ccccccgccc
       61 aggcgcaggc cggatgctag gctccccgtg tggctgaggc tgctgcccgg ctcccgagac
      121 cccgccgtcg ccgtcgctgg ctgatcatcg ccatcgcctc ggcggtcgcg ctgatcgcgc
      181 tcttgggcgg gctttcgggg ttctacatcg acctgctgtg gttccgggag gtcgggctgt
      241 ccccggtctt cgccagcatc ctctggacga agatcgtcct ggtcctgctg ttcgggctgg
      301 tcttcttcgc ggtcctgtac gccaacctgc tgatcgtgcg gcggacgagt ccgcgctacc
      361 ggctgttctc gccggagcag gaggtgatcg accgctatcg gcaagcggcc gatccgtaca
      421 tgcgctggat ccttccgggg ctgtcgctgc tgctggcgct gttcgcggcg gccagcgttg
      481 ccgggctgtg gcgcgagttc ctgctgtggc gcaacggcgc cgccgtccag ttcggcgtga
      541 acgacccggt gttccagcgc gacctctcct tctacgtgtt ccggctgccc ttcctgcact
      601 tcgtgcaggg gtggctgttc tcgtccctcg tggtgatcac ggtggtcacg gccgcggcgc
      661 actacctgtg gggcgggatc cgcttccagg cgatcgggga gaaggtcacg cccgttgtca
      721 aggcgcacct gtcggtgctg ctcggcgtga tcatgctggt gaaggcgtgg gggtaccggc
      781 tggggcagtt cgacctgctg agctcggagc gcggcgtggt gaccggggcc tcctacaccg
      841 acgtcaacgc ccagctcccg gcgctccgga tcctggtccc gatcgccatc ctcgtcgcgg
      901 tgctcttcct ggtgaacatc cggttccgcg ggtgggcgct cccgatcctc ggcatcggcc
      961 tgctcgcgct ggcgtcgatc gccgccgggg cggcgtaccc cgccttcgtc cagaagttcc
     1021 gcgtggaccc gcagccgctc gagcaggagc ggccgtacat cgaacgcaac atcgagttca
     1081 cgcgccgggc gttcgggctc gacggcgtgg agccgcggcg gatcgacgtc cagcccgacc
     1141 tgacgaaggg gaacgtggac gacaacccgc agaccgtgtc gaacatccgg ctgtgggagc
     1201 cggccctgct gcagcgcgcc taccagtcgc tgcagcagat ccggccgtac tactcgttcg
     1261 aggacgtgga cgtcgaccgc tacacgctgc ccgacggcac gcggcgggtg gtgatggtct
     1321 ccggacgcga gatcgcccaa tccgggatcc cggaggagag cgcgaactgg cagaaccgcc
     1381 atctgttctt cacccacggg tacggctacg tggccagccg cgtcgaccgc gtctccacgg
     1441 agggccagcc cgacttcctg gtgcgcggca tcccgccggg gggtgtcggg gccacctccg
     1501 gcgacctggc ggtcggctcg ccgcccgggc cgctgatgta ctacggcgag aacgaggacg
     1561 tcgggttcgt ggtggtcaag acgcggcaga aggagctcga ccgccccggg ggacagggtg
     1621 tcgcggaggg caccaccacc acgtacgccg gcgagggcgg catcgacctc ggcggcttat
     1681 tccgccggct ggcgttcgcc tggcactacc gggacgtgaa cctgctgatc tcgaacctga
     1741 tctccccgga ctcccggatc ctgatcaatc gcgacatcga ggaccgggtg cgcaaggccg
     1801 cgccgttcct cgacctggac cacgacccgt acatggcggt ggtcgacggc cggctggcgt
     1861 ggatctggga cgcctacacg accagcgacg cgtacccgta ctcgacgcgc ctgcaccccg
     1921 acgacctcgt ctccaccgac ggcggaccgg tgcgtctccc cggcagcgtg aactacatcc
     1981 gcaactcggt gaagatcgcc gtcgacgcct acgacgggac gatgacgttc tacatcgtcg
     2041 atcccgagga cccgatcgtg caggtctggc agaaggcgtt cccggagctg ttcgccccgg
     2101 cctcggcggt ctcgccgggc ctgcgggcgc acttccgcta cccggaggac ctgttcacgc
     2161 tgcaggccga gcagtacgcc aggtaccacg tgacggaccc ggacgacttc ttctccagcg
     2221 aggacttctg ggccgtgccg aacgacccga acgtggccga ttcgcgcgtg aagccgtact
     2281 acgtgctgat gcggctgccg ggcgacaccg aggaggagtt cgtcctgttc acgccgttca
     2341 ccccacggaa ccgcccgaac atgatcgcct ggctcgccgc ccgctcggac ccggatgcct
     2401 acgggcggat cacgcagatc gagttcgagg gcggccagac gatcttcggg cccgagcagg
     2461 tcgccgcgcg gatcaaccag gacccgaccg tgtcccagca gctcacgctg ctgaaccagc
     2521 agggctcgac cgtgcagtac gggccgctcc tggcgatccc gatcgaggag tcgttcctgt
     2581 acgtccagcc gctgttcctg gaggcctcgg cgggcggcgg gggcgcgatc ccggagctgc
     2641 agcgcgtggt cgtggtgcac ggcgagtcgg tgacgatcgg ggacaccctg caggagacga
     2701 tcgcgcagtc cttcgggctc gagccgcagg aaccgggggg cggggagcct cccgggcaga
     2761 cgatccagga gctgctggat caggcctcac ggcacttcgc ccgcgccgac cggctgctgc
     2821 gccagggcga cctggccgga taccagagcg agatcgacct ggcccgcgcc gcggtcgagc
     2881 aggcgctgga gctggcccag gggccggacg gcggcccgcc ggcgagcccg acgcccagcc
     2941 cgtccgccac cggttgagcg ggcgggacgg tcgccgggcc ctctgctagc atgggtttgc
     3001 ggggtggagc agtcaggtag ctcgctgggc tcataaccca gaggtcacgg gttcaaatcc
     3061 cgtccccgcg acgagccgaa gccgcaggtc atggacctgc ggcttctttc acgagcgcac
     3121 gaagtggcct cgattcggcc caaactgcaa acggattgca aacggagcaa ggcaagcatc
     3181 cgaacagcac caccgacttc attctgccca gggcacgcct cctcgagatg gtcggcgatc
     3241 accggcaagc ccaccaatgc ttgtcctctc cgcggctccc agctagcctc ccggagcaac
     3301 gctaccttca aacggggggc ggaacgactg tgcgtgcggt gatcgagact gatctagagt
     3361 tgggcggcgg tcgcaggcta cacgtgtacg acacgggcgt gaaggacaac cacagcctga
     3421 cggtcttctg gcatcacggc accccgaaca tcggcccccc tcccgaaccc ttgttcccgg
     3481 tctccgccga gctcgggatc cgctgggtct cgtatgatcg acctggctac ggcgggtcaa
     3541 ccagggatcc aggccgggac atcgcatcgg cggccgccta tgtcgctgcc gttgctgatg
     3601 agctgagcat cgaccggttc gcggtgatgg ggcactccgg cggcggtccg cacgctctgg
     3661 cttgtggcgc actgctgccg gcacgtgttc tgggggcggt cagcatgtcg ggcctggcgc
     3721 cgtacggcgc ggagcggctc gactggttcg cgggcatgtc ggcggccgga gtcgcttcgt
     3781 tgcgtgctgc cgccgccggc cgccaggcga aagaggcgta cgaggcttcg ccccccgcgg
     3841 gggacctggg gttcactgac gcggacgagg cggcactctc gggcgcgtgg tcctggctga
     3901 tcgacgtcgt acgtcccgcg attgcgggcg
//
LOCUS       DASPWO010000428         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_992566, whole genome shotgun sequence.
ACCESSION   DASPWO010000428 DASPWO010000000
VERSION     DASPWO010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992566"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /gene="hisS"
                     /locus_tag="VEO00_09730"
     CDS             <1..1154
                     /gene="hisS"
                     /locus_tag="VEO00_09730"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HYU58314.1"
                     /translation="RYVETPAFESTDLFARTSGESSDVVSKEMYTFPDRSGRSLTLRP
                     EGTAPVVRAYLAHAHSLGSPFKAYYVNQTWRYGRPQAGRLREFRTFGAEVLGVRSAEA
                     DVEVVVLADRFLRDLGLSRLELQVNSIGDETCRPAYRELLIEYLERNRERLRDEHRDR
                     FRANPLRVLDCKDDACREVSAGAPKIVDHLCEPCRQHHEEFLSALESEGVKAVPTPTL
                     VRGLDYYTRTAFEFVSDVLPKAQAGTVCGGGRYDRLAEVLGGPPTPGVGFGLGLDRTL
                     LAAHGEGIELAPAPGVTCFVVGVGAGRARGYDLLRQLRASGVSADAPFEERPLGAQLR
                     MANRSGARFALILGDEEVAEGTVTVRRMEDGHQERVPATDVPVWISAQA"
     gene            1133..>2358
                     /gene="aspS"
                     /locus_tag="VEO00_09735"
     CDS             1133..>2358
                     /gene="aspS"
                     /locus_tag="VEO00_09735"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HYU58315.1"
                     /translation="MDLRTSLRTHACGELRPEHAGGEVALCGWVAHRRDHGGVIFLDL
                     RDRMGIVQVVFHPEEAPEAYRAADATRGEWVVRVEGEVRMRPEGTANPNLGTGEVEVA
                     ARSLRVLSEAEPPPFPIEDRIEADETTRLQFRYLDIRRPEMTRVFELRARVNGVIRRY
                     LDERRFAEIETPMLTRSTPEGARDFLVPARLQPGSFYALPQSPQLFKQILMVAGLDRY
                     YQIVRCFRDEALRADRQQEFTQLDVEMSFVEEEDVFALLEPLMASIVRETHGVEVETP
                     FPRLPHDEALARYGSDKPDLRYGLELVDLSNAFAQTGFRAFESVLAGGGEIKAIRVPG
                     GSSLSRRELDDLVTEAKGRGAAGLVWMAFERSDIRSPVSRHLTPDELGAVASATGAGD
                     GDLVLVVADRPDRVAVA"
BASE COUNT          322 a          807 c          907 g          322 t
ORIGIN      
        1 atcggtacgt ggagacgccg gcgttcgaga gcacggatct ctttgcccgg acgtccggcg
       61 agtccagcga cgtggtgagc aaggagatgt acacgttccc ggaccggagc ggccggtcgc
      121 tcacgctgcg gccggagggc acggcgcccg tggtccgcgc ctacctcgcg cacgcacaca
      181 gcctgggctc gccgttcaag gcgtactacg tgaaccagac ctggcggtac gggcggccgc
      241 aggcgggacg gctccgtgag ttccggacct tcggggcgga ggtgctgggc gtgcggagcg
      301 cggaggcgga cgtcgaggtg gtcgtgctgg ccgaccggtt cctgcgcgac ctcgggctgt
      361 cgcggctgga gctccaggtg aacagcatcg gggacgagac ctgccggccg gcgtaccgtg
      421 agctgctgat cgagtacctg gaacgcaacc gcgaacgcct ccgcgacgag caccgcgacc
      481 ggttccgggc caacccgctc cgcgtgctgg actgcaagga cgatgcgtgc cgcgaggtct
      541 cggccggcgc acccaagatc gtggaccacc tgtgcgagcc gtgccggcag caccacgagg
      601 agttcctgtc ggccctcgag tcggagggcg tgaaggcggt gcccacgccg acgctggtgc
      661 ggggcctcga ctactacacg cggaccgcgt tcgagttcgt gtcggacgtc ctgccgaagg
      721 cgcaggccgg gacggtgtgc ggcgggggcc gctacgaccg gctggccgag gtcctgggcg
      781 gcccgccgac gcccggcgtg gggttcggcc tggggctgga ccgcacgctg ctcgcggcgc
      841 acggggaagg gatcgagctg gcacccgccc ccggcgtgac gtgcttcgtc gtgggggtcg
      901 gggccgggcg ggcaagaggc tacgacctcc tgcgccagct ccgtgcctcc ggggtctccg
      961 cggacgcgcc cttcgaggag cggcccctcg gagcgcagct tcggatggcc aaccgcagcg
     1021 gcgcgcggtt cgcgctcatc ctgggcgacg aggaggtcgc cgagggcacc gtcaccgtgc
     1081 ggcgcatgga ggacggccac caggagaggg tcccggccac ggacgttccg gtatggatct
     1141 ccgcacaagc ctgaggacgc acgcctgcgg ggaactccgc ccggagcacg ccgggggcga
     1201 ggtcgcgctg tgtggatggg tcgcccaccg ccgcgaccac ggcggcgtca tcttcctgga
     1261 cctccgggac cggatgggga tcgtgcaggt cgtattccat ccggaggagg ccccggaggc
     1321 gtaccgggcg gccgacgcca cgcggggcga gtgggtggtg cgggtcgagg gagaggtccg
     1381 tatgcgcccc gaggggacgg cgaaccccaa cctggggacg ggggaggtcg aggtcgcggc
     1441 gcgctcgctc cgggtcctgt ccgaagcgga accgccgccg tttcccatcg aggaccgcat
     1501 cgaggccgac gagaccaccc ggctccagtt ccggtacctc gacatccgcc ggcccgagat
     1561 gacccgggtg ttcgagctcc gggcgcgggt gaacggggtc atccgccggt acctggacga
     1621 gcgccggttc gccgagatcg agacgcccat gctcacgcgg agcacgcccg agggcgcgcg
     1681 ggacttcctg gtgccggccc ggctccagcc gggctcgttc tacgcgctgc cccagtcgcc
     1741 gcagctgttc aagcagatcc tcatggtggc cggcctcgac cgctactacc agatcgtgcg
     1801 gtgcttccgg gacgaggccc tgcgcgccga ccggcagcag gagttcaccc agctcgacgt
     1861 cgagatgtcg ttcgtcgagg aggaggacgt gttcgcgctg ctcgagcccc tcatggcgtc
     1921 gatcgtccgc gagacgcacg gcgtcgaggt ggagacgccg ttcccccggc tgccccacga
     1981 cgaggccctg gcccggtacg gaagcgacaa gccggacctc cggtacgggc tcgagctcgt
     2041 cgacctgtcg aacgcgttcg cccagaccgg cttccgggcc ttcgagtccg tgctggccgg
     2101 gggaggagag atcaaggcga tccgggtgcc cggcggctcg tccctgtcgc gccgcgagct
     2161 cgacgacctg gtcacggagg cgaagggccg gggcgccgcg gggctcgtgt ggatggcgtt
     2221 cgaacgctcg gacatccgct cgccggtgag ccggcacctg accccggacg agctcggcgc
     2281 ggtggcctcg gcgaccggcg ccggggacgg cgacctcgtc ctcgtcgtgg cagaccgtcc
     2341 cgatcgggtg gccgtggc
//
LOCUS       DASPWO010000429         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1246418, whole genome shotgun sequence.
ACCESSION   DASPWO010000429 DASPWO010000000
VERSION     DASPWO010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246418"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /gene="murD"
                     /locus_tag="VEO00_09740"
     CDS             complement(<1..1343)
                     /gene="murD"
                     /locus_tag="VEO00_09740"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HYU58316.1"
                     /translation="MHDPTGGGWGGSPPHEPVSGGAFAGERAVVIGLGLAGAAAARVL
                     AEEGASVRVSEARPEAAADPALAALGVEVLGGEHRPEHLDGATLVVTSPGVPEGAPVL
                     AWARDRDLPVWSELEIGARMAPMPYVAVTGTNGKTTTTEMVAAAMREAGLAARACGNV
                     GYPFSVAVREPFDALAVEVSSFQLRFLESFHPRVSVLLNLSPDHLDWHGSVERYVAAK
                     RRILVNQGPGDAHVGNRDDALAGEISRGAAASGVDLRWFTLGPPGPGEVGYEGAALVS
                     RAGVKVPLGVPASPTASHRADAAAAAAAAIAFGLDAEAAGRAVCAFRPLPHRGEVVAS
                     VGRVRFVDDSKATNPHAALAAVEGATGVVLIAGGMAKGVDLSPLARAVPALAGVVAIG
                     QAADELVALFDGLVPVRRAAGMDEAVRAALEMAPPDGTVILAPACASQDMFRDYA"
     gene            complement(1352..2383)
                     /gene="mraY"
                     /locus_tag="VEO00_09745"
     CDS             complement(1352..2383)
                     /gene="mraY"
                     /locus_tag="VEO00_09745"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HYU58317.1"
                     /translation="MRPILFAAAAGLVFALFATPLAIRIFRAWGFGQRIREAWAGEGP
                     EAHHGHLEKMGTPTMGGLVILGGMVAGYAVSRLVLREVTATALCVFGAVLGLGLIGFL
                     DDFLKIRRQRSLGLNKTAKFLGQALIAVGFAFAAVEFAGASTFLSFIRESGYDLGLFF
                     YVWAVVIFAASSNGVNLTDGLDGLASGSMTMVMAAYVIIAFWQFRHPGAYDVRDALDV
                     AVIAAGALGACAGFLWFNAAPARIFMGDTGSLALGGLAATMAIFTNTHLLLIVIGGLF
                     VLETASVVIQVVSFRFFHRRVFRMAPFHHHFELIGWPEFTVIVRFWIICGLAVAFGLG
                     LFYAEFLGQ"
     gene            complement(2380..3759)
                     /gene="murF"
                     /locus_tag="VEO00_09750"
     CDS             complement(2380..3759)
                     /gene="murF"
                     /locus_tag="VEO00_09750"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HYU58318.1"
                     /translation="MRARPVTAIAALVGGAVRGDGTVRVDRVVADSREAVPGALFVAL
                     PGERADGHDFVAEAAARGASAALLARDGRWPVPAVVVSHPGAALLDLAADERRAMGAA
                     VVGITGSSGKTCTKDFTAAVLSIARRTHASPRSFNTEVGVPVTILGAPDGTETLVLEM
                     GSRGIGHVAALCAVARPDVGVVTNVGPAHLATFGSRENVARAKAELVEALPQSGVAVL
                     NADDPVVAGFASVSRARVVTYGVGGHADVRAEDVSLDEGARAAFTLAADGGAERVELT
                     VPGEHMVPNALAAAAAGLALGLGLGECAAGLKDARVSAWRMEVVEARGGFRILNDAYN
                     ANPASTAAALKAARWMARERRCVAVLGEMAELGDTAYEEHERLGETVARLGIEELVTV
                     GERGRIIARGAIREGVEEDHVTMCADGQEALAAVRRIVRPGDVVLVKASRVAGLEWLA
                     GALARRGAG"
     gene            complement(3756..>4036)
                     /locus_tag="VEO00_09755"
     CDS             complement(3756..>4036)
                     /locus_tag="VEO00_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HYU58319.1"
                     /translation="GGGAYVIEPDRRAAIRLAVRDAGPGDVVVIAGKGHETGQEFADR
                     TIPFDDRAVARDELSRLAGGSGGEARRGRDRHGDVRRADPPGKRDGRP"
BASE COUNT          582 a         1633 c         1382 g          439 t
ORIGIN      
        1 tcggcgtagt cgcggaacat gtcctggctg gcgcaggcgg gcgccaggat caccgtcccg
       61 tccggcggcg ccatctcgag cgcggcgcgc acggcctcgt ccatcccggc ggcccggcgc
      121 accggcacga ggccatcgaa cagcgccacc agctcgtcgg cggcctgccc gatggcgacc
      181 acgccggcca gcgccggcac cgcccgcgcc agcggcgaca ggtccacgcc cttcgccatc
      241 ccgccggcga tcagcaccac gccggtggcg ccctccaccg ccgcgagcgc ggcgtggggg
      301 ttcgtcgcct tcgagtcgtc cacgaagcgg acccggccga ccgaggccac gacctcgcca
      361 cggtgcggga ggggacggaa ggcgcagacg gcgcgcccgg cggcttccgc gtccagcccg
      421 aaggcgatcg cggcggcggc ggccgcggcc gcgtcggctc ggtgggacgc cgtcggggac
      481 gccgggacgc cgagggggac cttcacgccc gcgcgagaga cgagcgccgc cccctcatag
      541 cctacctccc ccggccccgg ggggccgagg gtgaaccagc gaaggtccac cccggaggcg
      601 gccgctcccc gcgagatctc ccccgccagg gcgtcgtcgc ggttgcccac gtgcgcgtcg
      661 cccggtccct ggttcaccag gatgcgccgc ttcgccgcga cgtagcgctc gaccgacccg
      721 tgccagtcga gatggtcggg cgacaggttc agcagcaccg acacgcgcgg gtggaaggac
      781 tccaggaacc gcagctggaa cgacgacacc tcgacggcca gggcgtcgaa cggctcgcgg
      841 accgcgaccg agaacggata gccgacgttc ccacaggccc gggcggcgag gccggcctcc
      901 cgcatcgccg cggcgaccat ctcggtggtg gtggtcttgc cgttcgtccc ggtaacggcc
      961 acgtacggca tgggcgccat gcgcgccccg atctcgagct cgctccacac cggcaggtcg
     1021 cgatcgcgcg cccaggccag cacgggagcg ccctcgggaa cgcccgggct cgtcaccacg
     1081 agcgtcgcgc cgtcgaggtg ctcgggccgg tgctcgccgc cgagcacctc cacgcccagc
     1141 gcggcgagcg ccggatcggc ggcggcctcc ggacgggcct cgctcacccg caccgaggcg
     1201 ccctcctcgg ccagcacgcg cgcggccgcc gcgccggcca ggccgaggcc gatcaccacg
     1261 gcccgctcac cggcgaaggc gccgccgctc accggctcgt ggggaggaga ccctccccac
     1321 cccccacccg tcgggtcgtg catgccgaag gtcattggcc caggaactcc gcgtagaaga
     1381 ggccgaggcc gaaggccacg gccagcccgc agatgatcca gaagcggacg atcaccgtga
     1441 actccggcca gccgatcagc tcgaagtgat ggtggaacgg cgccatccgg aacaccctgc
     1501 ggtggaagaa gcggaacgag acgacctgga tcaccaccga cgcggtctcg agcacgaaca
     1561 ggccgccgat cacgatcagc agcaggtgcg tgttcgtgaa gatggccatc gtcgccgcga
     1621 ggcctccgag cgccagcgag ccggtatcgc ccatgaagat ccgcgccggc gcggcgttga
     1681 accacaggaa gcccgcgcac gcgccgagcg ccccggcggc gatcactgcg acgtcgagcg
     1741 cgtcgcgcac gtcgtacgcg cccgggtggc ggaactgcca gaacgcgatg atcacgtagg
     1801 ccgccatgac catggtcatc gatcccgatg ccaggccgtc cagcccgtcg gtcaggttca
     1861 ccccgttcga cgaggcagcg aagatcacga cggcccacac gtagaagaag aggccgaggt
     1921 cgtaccccga ctccctgatg aacgagagga aggtggacgc cccggcgaac tcgacggccg
     1981 cgaacgcgaa gcccaccgcg atcagcgcct ggccgaggaa cttcgcggtc ttgttcaggc
     2041 cgagcgaccg ttgccgccgg atcttcagga agtcgtccag gaacccgatc agccccagcc
     2101 cgagcaccgc gccgaagacg cacagcgcgg tggccgtcac ctcgcgcagg acgagccgcg
     2161 acaccgcata gccggcgacc atcccgccaa ggatcaccag cccgcccatc gtcggcgtcc
     2221 ccatcttctc caggtggccg tggtgcgcct ccggcccctc gcccgcccac gcctcgcgga
     2281 tcctctggcc gaagccccag gcgcggaaga tccggatggc gagcggcgtg gcgaacaggg
     2341 cgaagaccag accggccgcg gccgcgaaca ggatcggcct catccggctc cccgcctcgc
     2401 cagggcgccg gccagccact ccaggccggc gacgcgggag gccttcacca gcaccacgtc
     2461 cccggggcga acgatccgcc gaaccgccgc cagcgcctcc tgcccgtcgg cgcacatcgt
     2521 gacgtggtcc tcctccacgc cctcgcggat cgcgccgcgg gcgatgatcc gtccgcgctc
     2581 gccgaccgtg accagctcct cgatccccag gcgggccacc gtctcaccga gccgctcgtg
     2641 ctcctcgtac gcggtgtcgc ccagctcggc catctcgccg agcaccgcca cgcagcggcg
     2701 ctcgcgggcc atccatcgcg cggccttcag cgcggcggcg gtggaggccg ggttcgcgtt
     2761 gtacgcgtcg ttcaggatcc ggaacccgcc gcgcgcctca accacctcca tccgccacgc
     2821 cgacacccgg gcgtccttca gcccggcggc gcactcgccg aggccgaggc cgagcgccag
     2881 cccggccgcc gccgccgcca gcgcgttcgg gaccatgtgc tcgccgggca ccgtcagctc
     2941 gacgcgctcg gcgccgccgt ccgcggccag cgtgaaggcc gcccgggcgc cctcgtccag
     3001 cgagacgtcc tcggcgcgaa cgtcggcgtg cccgccgacg ccgtaggtca ccacccgcgc
     3061 cctgctcacc gacgcaaagc ccgccaccac cgggtcgtcc gcgttcagga cggcgacgcc
     3121 ggactgcggg agcgcctcca ccagctcggc cttcgcccgg gcgacgttct cgcgcgagcc
     3181 gaacgtcgcc aggtgggccg ggccgacgtt cgtcaccaca ccaacgtccg ggcgggccac
     3241 cgcgcagagc gccgcgacgt ggccgatccc ccggctcccc atctcgagca ccagcgtctc
     3301 ggtcccgtcc ggcgcgccca ggatcgtcac cggcaccccg acctcggtgt tgaacgagcg
     3361 cgggctggcg tgcgtgcgcc gggcgatgga gagcaccgcg gcggtgaagt ccttggtgca
     3421 ggtcttgccc gaggagccgg tgatccccac gacggcggcg cccatcgccc ggcgctcgtc
     3481 ggccgccagg tccagcaacg ccgcgcccgg gtgggacacg accacggccg gaaccggcca
     3541 gcggccgtcg cgggcgagga gcgccgcgct ggctccccgg gcggcggcct cggcgacgaa
     3601 gtcgtgcccg tccgcacgct cgccgggcag cgcgacgaac agcgcgccgg gaaccgcctc
     3661 ccggctgtcg gccacgaccc ggtcgaccct gacggtgccg tcgccccgca ccgcaccgcc
     3721 aacgagcgcg gcgatcgccg tcaccggccg tgccctcacg gccgcccgtc cctcttgcct
     3781 ggggggtccg ctcgccggac gtcgccgtgc cggtctctcc cacggcgagc ttccccccca
     3841 gaccccccgg ccagccggga gagctcgtcg cgggccacgg cccgatcgtc gaacgggatc
     3901 gtccggtcgg cgaactcctg cccggtctcg tggcccttcc ccgcgatgac caccacgtcg
     3961 ccgggcccgg cgtcccggac cgcgaggcgg atcgcggcgc gccggtccgg ctcgatcacg
     4021 tacgcaccgc cgccgc
//
LOCUS       DASPWO010000430         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1083204, whole genome shotgun sequence.
ACCESSION   DASPWO010000430 DASPWO010000000
VERSION     DASPWO010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083204"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VEO00_09760"
     CDS             <1..1010
                     /locus_tag="VEO00_09760"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HYU58320.1"
                     /translation="ERTEVIELAGARVPALLLPNDRDLTFAKVRFDERSLATIETHLG
                     GLPESLSRAVVWSALWDMARDAELPAGRYLRIIHAHASRESHVGVLESVLGHAASLID
                     LLGDPSRRISRGEGFAALALERLRAAEPGSDAQLAWARAFIGAARSADQLADVRGLLD
                     GTESVEGLEVDIDLRWHIVRSLAAAGAADEELIAAEERRDPTDEGARNAAAARAAQPE
                     PAAKEAAWARAFDAAASFSEVVDVLAGFQRAGQEELLRPYRERYFETLGEVWAERDIQ
                     VALMLGVRAYPHNVVEPETMAATEAYLARGDVPGPVRRLLLEAKDGVARALRTRAADR
                     G"
     gene            complement(1026..>2296)
                     /locus_tag="VEO00_09765"
     CDS             complement(1026..>2296)
                     /locus_tag="VEO00_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58321.1"
                     /translation="TARWLSAAALAVSVVATGAIVRTATGSIVAAGVAAALTLSGTAI
                     LLVHAFAWSEPPFIATSLLSSWLLLRHLRSPRRRALIAAAALAGAAWLIRYAGFGLVA
                     AGAVALGVAGPRTGRERLSDVWRFLAVSIAPALLWAAATVVGSGSVAGRRLGWHPPGP
                     EALGAGARALAAWVLPAAVPAAAGVTLVLGLVAVALAQSVAPGSAPDRAFAGMAGRFL
                     LIVAGAHVAVTAITMTFLDAATVPDARILAPAFPFVVAGIAAVAHAPVAALVSSGGAR
                     RALALAIGVVLLAVPAVRAVAWVGGRREQALGFTSERWQASPLLGAVRGLPPDAVVFT
                     TDTAVVYWITGRVPRPLPLRFDPHTLDANPDYRAELQRMGRDLQASGGAVALFDPMPP
                     HLPSEAELRKLLGLVTAGEFADGRLLVAPD"
BASE COUNT          312 a          860 c          895 g          229 t
ORIGIN      
        1 gggagcggac cgaggtgatc gagctggccg gcgcgcgcgt cccggccctc ttgctgccga
       61 acgaccgcga cctgacgttc gccaaggtcc gcttcgacga gcggtcgctg gccacgatcg
      121 agacccacct cggcgggctc cccgagtcgc tgtcgcgcgc cgtcgtgtgg tcggcgctgt
      181 gggacatggc ccgcgatgcc gagctcccgg ccgggcggta cctgcgaatc atccacgccc
      241 acgcgtcccg cgagtctcac gtcggcgtgc tcgagagcgt gctgggtcac gcggcgtcgc
      301 tgatcgacct gctcggcgac ccatcccgcc ggatctcccg cggtgagggc ttcgcggcgc
      361 tcgcgctgga gcgcctgcgc gcggccgagc cggggagcga cgctcagctg gcctgggccc
      421 gggccttcat cggcgccgcc cgatcggccg atcagctggc agacgtccgc ggtctgctcg
      481 acgggaccga gtcggtcgag gggctggagg tggacatcga tctgcgctgg cacatcgtcc
      541 gctcgctggc ggcggccggc gccgccgacg aggagctgat cgcggccgag gagcggcggg
      601 acccgaccga cgagggcgcc cgcaacgcgg cggcggcgcg cgccgcgcag ccggagccgg
      661 cggcgaagga ggccgcctgg gcccgggcgt tcgacgcggc ggcctcgttc agcgaggtgg
      721 tcgacgtgct ggccggcttc cagcgggcgg gccaggagga gctcctgcgg ccctaccggg
      781 agcgctactt cgagacgctc ggggaggtct gggcggagcg cgacatccag gtcgcgctca
      841 tgctcggcgt gcgcgcctac ccgcacaacg tcgtcgagcc ggagaccatg gccgcgaccg
      901 aggcgtacct cgcccgtggc gacgtcccgg gcccggtccg ccgcctcctg ctcgaggcga
      961 aggacggcgt ggcccgcgcc ctgcgcaccc gcgccgccga ccggggctga gccgcgcccg
     1021 gccgctcaat cgggcgcgac gaggagccgc ccgtcggcga actccccggc cgtcaccagg
     1081 ccgagcagct ttcgcagctc ggcctccgag ggcaggtggg gaggcatcgg gtcgaagagg
     1141 gcgacggctc cgccggacgc ctggaggtca cgccccatcc gctgcagctc ggcccgatag
     1201 tcgggattcg cgtcgagggt gtggggatcg aagcgcaagg ggagtgggcg cggcacccgg
     1261 ccggtgatcc agtagacgac ggccgtgtcg gtggtgaaca ccaccgcgtc cggggggagc
     1321 ccccgcacgg cgcccaacag cggtgaggcc tgccaccgtt cggaggtgaa gccgagggcc
     1381 tgctcccgcc gtccccccac ccaggccacc gcccggaccg ccggcacggc gagcaggacc
     1441 acgccgatgg ccagcgccag ggcccggcgt gccccgccgg aggacaccag ggcggcgacc
     1501 ggggcgtggg cgacggcggc gatccccgcg acgacgaacg ggaaggccgg ggccaggatg
     1561 cgcgcgtccg gcaccgtcgc ggcatcaagg aacgtcatcg tgatcgccgt cacggcgacg
     1621 tgcgcccccg ccacgatcag caggaacctg ccggccatgc cggcgaacgc ccggtccgga
     1681 gcgcttcctg gcgccacgct ctgcgccagc gccacggcca ccagccccag gaccagcgtc
     1741 acgccggccg cggccgggac ggcggcgggc aggacccagg ccgcgagggc acgggcaccg
     1801 gcgccgagcg cctccgggcc cggcggatgc cagccgaggc gccggccggc gaccgacccc
     1861 gaaccgacga cggtggcggc cgcccacagg agggcgggcg cgatggacac ggccaggaac
     1921 cgccagacgt cgctcaggcg ctcgcggccc gtccgcggcc cggcgacgcc gagggcgacg
     1981 gcgcccgcgg cgacgagccc gaatcccgcg tagcggatca accaggcggc cccggccaag
     2041 gcggcggccg cgatcagcgc gcgacggcgc ggggaccgca ggtgccgcag gagcagccag
     2101 gaggagagga gcgaggtggc gatgaacggg ggctcgctcc atgcgaacgc atggacgagc
     2161 aggatcgcgg tcccggagag cgtcagggcc gccgcgacgc ccgcggcgac gatgctcccg
     2221 gtcgcggtcc ggacgatcgc gccggtcgcc accaccgaca ccgcgagcgc tgccgcgctc
     2281 agccacctgg cggttt
//
LOCUS       DASPWO010000431         5551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1083214, whole genome shotgun sequence.
ACCESSION   DASPWO010000431 DASPWO010000000
VERSION     DASPWO010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083214"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VEO00_09770"
     CDS             complement(<1..214)
                     /locus_tag="VEO00_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HYU58322.1"
                     /translation="MTEAASGPGVRIFINYRRDDTAGHAGRLYDSLVEEFGDGSVFMD
                     IDTIDPGEDFVEVIHEGVGSCDVVLSL"
     gene            complement(227..1480)
                     /locus_tag="VEO00_09775"
     CDS             complement(227..1480)
                     /locus_tag="VEO00_09775"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HYU58323.1"
                     /translation="MSEAAGRPDVRIFISYRRDDAAGHAGRLYDALVEHYGEDKVFMD
                     LAVIEPGVDFVDVIHEGVGSCDVVLTLIGRNWLAATDAKGRRRLENPEDFVRLELETA
                     LHREIRVIPLLVQGAQMPGSDELPATLSRLARRNGLELSDPRWSYDVGRLIVAIDRIA
                     TEKASRVARGGPAATEPPTIPPVPPPPGPATVPIPPMVAPPPGTPSAPPARGRARNVV
                     PIVAGVVVLAGVVLLVVFLGGGGDERATPTTAPPSTAVSPSVTTSPSPTTSATPSDAA
                     AVGQQFLLDHVPADHVDSCAADTEFLPDGATAGIKCSPNDNVTFWYYLYSGDTDLQAW
                     YSKRLGDESITRDVGDCQTDDTSESQLFVAGEATRGRFVCYTDENGRWVEWTRPELLI
                     YGYLNRPDGKRQATYDFWSGAGPTV"
     gene            complement(1503..2450)
                     /locus_tag="VEO00_09780"
     CDS             complement(1503..2450)
                     /locus_tag="VEO00_09780"
                     /inference="COORDINATES: protein motif:HMM:NF033610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HYU58324.1"
                     /translation="MSERDIAADPPDVGPGGPLTLGERDFPELFDAADRASAGAQRAF
                     VRSTAAQLLLLVLAAVAAAVSAQVGRTDVAAAIAALAFAAAASLKTHLLTTKPDKTWY
                     QGRAAAESAKTLAWRYAVGGEPFRADAPDPVRLDRQFLERLGEILRPLDDIALIPSVE
                     RSDQITPWMRRLRGRSLEERRRAYREGRILDQQRWYATKAAYNDKRARQWGAVMLAFE
                     LAGVVLLLARAVGLLTLDLGGIAAAVAGTAAAWVQTKQHAALAAAYSVTARELGQVAV
                     EVDDPTTEDEWAKFVGHAEDAVSREHTLWLASRGKKADE"
     gene            complement(2672..3409)
                     /gene="uppS"
                     /locus_tag="VEO00_09785"
     CDS             complement(2672..3409)
                     /gene="uppS"
                     /locus_tag="VEO00_09785"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HYU58325.1"
                     /translation="MSYRSDLDLRHLPRHVAIVMDGNGRWAKQRGLPRTEGHAAGEEA
                     LFDTVEGALDIGLRWLTVYAFSTENWHRPKSEVAYLMNFNRGLLQRRRDELHERGARI
                     RFLGRENWRVPRSVLREMEVARELTKHNTKMTLTIAFNYGGRTEIVDAVKRLLEDHDR
                     GRLKGERITPDSIARRLYHPDMPDPDLLIRTGGEMRISNFLLWEAAYAELWFTPVFWP
                     DFNREHLFEAIRDYQKRDRRFGRVNGD"
     gene            complement(3414..4253)
                     /gene="recO"
                     /locus_tag="VEO00_09790"
     CDS             complement(3414..4253)
                     /gene="recO"
                     /locus_tag="VEO00_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HYU58326.1"
                     /translation="MPLYKEQGVVLRAVKLAEADKIVTVLTQGAGKVRAVAKGIRRTT
                     SKFGARLEPLTHVSLLLYQGRNLDTITQAEILSPFRAVRDDFALFSAGEAMLETADKV
                     AEEHERNVPLFLLLLNGLRALDARPADPAATAEAYLLRLLSLSGFHPALDACASCGSP
                     DVAAFSPAQGGTVCAGCAEASAAPARPGALAWLRTLAGTDLARAGEEPSPAPDDRLAA
                     RLLLFGFAEYHLERRIRAFPMLARTSPRTSPLGGPLAARGRVDPSTGRPTPPHPPALL
                     PAP"
     gene            4396..5181
                     /locus_tag="VEO00_09795"
     CDS             4396..5181
                     /locus_tag="VEO00_09795"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYU58327.1"
                     /translation="MMNSQAAAAVLDRDAPLPLYFQLRQAVLLEARERGLEPGDRLPT
                     EADLESRYGVSRTTIRQALSELEAEGLVRRVQGKGTFLGTPRIQHVPVLASFSELLRS
                     QGFDPSHRLLASAVRPALPEVAEGLGLEAGAECRFLRRLFLADGDPVGLAETWLPRAL
                     LGPSDALFQKGEIEDGSLYELLQRPPIGLVLHRAMETISPDVAAAADARRLRCPPGSP
                     LLVIVRVTFTPDDRPVEYTRLLFAGDRYEYRVEMQRPAEIRRR"
     gene            5183..>5551
                     /locus_tag="VEO00_09800"
     CDS             5183..>5551
                     /locus_tag="VEO00_09800"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HYU58328.1"
                     /translation="METRDVREVIAAVVGGLVGEGNGAPVGGPDNVPAADGEPAPPRR
                     PAGGLAEIAVGSDHAGFLLKQRIAADLLEHGFTVHDCGPEAPDPVDYPDYAHAVARLV
                     ADGDCRWGIVIDGAGIGSAIA"
BASE COUNT          732 a         1976 c         2065 g          778 t
ORIGIN      
        1 tcagcgacag cacgacgtcg caggacccca cgccctcgtg gatgacctcg acgaagtcct
       61 cccccgggtc gatcgtgtcg atgtccatga acacgctgcc gtcgccgaac tcctcgacca
      121 gggagtcgta cagccggccg gcgtgccccg cggtgtcgtc gcggcggtag ttgatgaaga
      181 tccgcacgcc cggcccggac gcggcctcgg tcatatcacc tcccgatcac accgtcggcc
      241 cggcgccgct ccagaagtcg tacgtggcct ggcgcttccc gtcgggacgg ttgaggtagc
      301 cgtagatcag gagctccggg cgggtccact ccacccaccg cccgttctcg tccgtgtagc
      361 acacgaaccg gccgcgggtc gcctctccgg cgacgaagag ctgtgactcc gacgtgtcgt
      421 cggtctggca gtcgccgacg tcgcgcgtga tcgactcatc gccgaggcgc ttcgagtacc
      481 aggcctgcag gtcggtgtcg ccgctgtaga ggtagtacca gaacgtcacg ttgtcgttcg
      541 gcgagcactt gatgccagcg gtggcgccgt cgggcaggaa ctcggtgtcg gcggcacagg
      601 agtccacgtg gtcggccggg acgtggtcga gcaggaactg ctgccccacg gccgcggcgt
      661 cggacggagt cgcgctcgtg gtggggctgg ggctggtggt cacggacggc gacaccgcgg
      721 tggacggtgg tgcggtcgtc ggggtcgcgc gctcgtcgcc gccgccaccg aggaacacga
      781 ccagcagcac gacgccggcc aggacgacga ccccggcgac gatcgggacc acgttccgtg
      841 cgcgtcctcg cgccggcggc gcggagggcg ttcccggcgg cggggccacc atcggcggga
      901 tggggacggt ggccggccct ggcggcggag ggaccggagg gatcgttggc ggctcggtgg
      961 ccgccggccc gccccgggcg acgcgcgagg ctttctcggt cgcgatccgg tcgatcgcga
     1021 cgatcaggcg gccgacgtcg taggaccagc gcgggtccga aagctccagc ccgttgcgcc
     1081 gggccagccg cgacagcgtg gccggcagct cgtccgagcc cggcatctgc gctccctgca
     1141 cgagcagcgg gatcacgcgg atctcgcggt gcagcgcggt ctcgagctcg aggcggacga
     1201 agtcctccgg gttctccagg cgccggcgcc ccttggcgtc ggtggccgcc agccagttcc
     1261 gcccgatcag ggtgagcacc acgtcgcacg acccgacgcc ctcgtgaatg acatcgacga
     1321 agtcgacgcc cggctcgatc accgcgaggt ccatgaacac cttgtcctcg ccgtagtgct
     1381 cgacgagcgc gtcgtacagc cgcccggcat gccccgcggc gtcgtcgcgg cggtagctga
     1441 tgaagatccg gacgtcgggc cggccggccg cttcgctcac ggccgcctct ccatggacgt
     1501 gctcattcgt ctgccttctt gccgcgggag gcgagccaca gggtgtgctc gcgggagacg
     1561 gcgtcctcgg cgtggccgac gaacttcgcc cactcgtcct cggtcgttgg gtcgtccacc
     1621 tcgacggcca cctggccgag ctcgcgcgcc gtgaccgagt acgccgcggc gagcgcggcg
     1681 tgctgcttcg tctgcaccca ggcggcggcg gtcccggcca cggccgcggc gatcccaccg
     1741 aggtcgaggg tcaagagccc gaccgctcgc gccagcagca gcaccacgcc ggcgagctcg
     1801 aaggccagca tcaccgcgcc ccactgccgg gcccgcttgt cgttgtacgc cgccttggtg
     1861 gcgtaccagc gctgctggtc caggatccgg ccctcccggt acgcccgccg ccgctcctcg
     1921 agcgaccggc cgcgcaggcg tcgcatccac ggcgtgatct ggtcggagcg ctcgaccgac
     1981 gggatcagcg cgatgtcgtc gagcgggcgc aggatctccc ccagccgctc caggaactgc
     2041 cggtcgaggc ggaccggatc cggcgcgtcc gcccggaagg gctctccgcc gaccgcgtac
     2101 cgccaggcca gggtcttcgc cgactcggcc gccgcccgtc cctggtacca ggtcttgtcc
     2161 ggcttcgtcg tcagcaggtg tgtcttgagc gatgccgccg cggcgaacgc cagggcggcg
     2221 atcgccgccg cgacgtccgt ccggccgacc tgggccgaca cggcggcggc cacggcggcg
     2281 agcacgagca gcaggagctg ggcggcggtg gaacgcacga acgcgcgttg cgcgccggcc
     2341 gacgcgcgat ctgccgcgtc gaagagctcc gggaagtcgc gttcgccgag cgtcagcggg
     2401 ccgccgggac cgacgtccgg ggggtcggcg gcgatgtcgc gctcgctcaa gggcagggtc
     2461 ctcccggtcg cgcccgcccg aggcagcggg gggcgaggac aagtctgcgc gagcccggac
     2521 gccggcgcaa tggccctctc gggccagccc ggcggttccc ggaacgcctg tcccgcgcct
     2581 cctcaggcgt ggcgaggggc ggcgtccggc aggtcgtgct cgagcacggg ctcgagggcc
     2641 acggtggtca gcgcgctacc ggccgcccgg atcagtcgcc gttcacgcgg ccgaagcggc
     2701 ggtcgcgctt ctggtagtcg cggatggcct cgaacaggtg ctcgcggttg aagtccggcc
     2761 agaacaccgg cgtgaaccac agctcggcgt aggccgcctc ccacagcagg aagttggaga
     2821 tgcgcatctc gccgccggtg cggatcagca ggtcggggtc gggcatgtcc gggtggtaca
     2881 ggcggcgggc gatcgagtcc ggcgtgatcc gctccccctt cagccggccg cgatcgtgat
     2941 cctccaggag gcgcttcacc gcgtcgacga tctcggttcg cccgccgtag ttgaaggcga
     3001 tcgtcagcgt catcttcgtg ttgtgcttcg tcagctcgcg cgcgacctcc atctcgcgga
     3061 gcaccgatcg cgggacccgc cagttctccc ggcccaggaa ccggatccgc gcgccgcgct
     3121 cgtgcagctc gtcgcgccgg cgctgcagca ggccgcggtt gaagttcatc aggtaggcca
     3181 cctcggactt cgggcggtgc cagttctcgg tcgagaacgc gtagacggtc agccaccgga
     3241 gcccgatgtc gagggcgccc tcgacggtgt cgaacagagc ctcctcgccg gcggcgtgac
     3301 cctcggtgcg cggcaggccg cgctgcttcg cccaccgccc gttgccgtcc atgacgatcg
     3361 ccacgtgccg gggcaggtga cgcaggtcga ggtcggagcg gtagctcatc ggctcagggg
     3421 gccggcagga gcgccggggg gtgtgggggg gtgggtcgcc ctgtggaggg atcgacccgg
     3481 cctcgtgcgg cgagcggacc ccccaggggt gaggttcgcg gcgacgtgcg ggcgagcatg
     3541 gggaaggccc tgatccgccg ctccaggtgg tactcggcga acccgaagag caggaggcgc
     3601 gccgccaggc gatcatcggg cgcgggcgag ggctcctcgc cggcgcgggc caggtcggtc
     3661 ccggcgagcg tgcgcagcca ggccagcgcc ccgggccgcg ccggcgccgc gctggcctcc
     3721 gcacacccgg cacagaccgt gccgccctgg gccggcgaga acgcggcaac gtccgggctt
     3781 ccgcaggacg cgcaggcgtc gagggcgggg tggaagccgg acagcgacag caggcgcagc
     3841 aggtaggcct cggccgtggc ggccggatcg gccggacggg cgtcgagcgc gcggaggccg
     3901 ttcagcagga gcaggaagag cgggacgttg cgctcgtgtt cctcggccac cttgtccgcg
     3961 gtctcgagca tggcctcgcc ggcggagaag agcgcgaagt cgtcgcggac cgcccggaac
     4021 ggggagagga tctccgcctg ggtgatcgtg tcgaggtttc gcccctggta gagcagcagc
     4081 gacacgtgcg tcagcggctc gaggcgggca ccgaacttgc tggtcgtgcg gcggatcccc
     4141 ttggcgaccg cccggacctt gccggccccc tgtgtgagga ccgtgacgat cttgtcggcc
     4201 tcggcgagct tgacggcacg caggacgacg ccctgttcct tgtagagggg catcgccgag
     4261 atcgtactac cggccctgcg cggaacccct ggacgagatg tgaagaaccc gtgcagagca
     4321 gtgcgagctt cagcggcgaa cctcttgaca ggtccggccg tttcgagcat catgatgacg
     4381 ggacatcgtg tcatcatgat gaattcgcag gcagcggcgg ccgtgctcga ccgggacgca
     4441 cccctcccgc tctacttcca gctgcgccag gcggtgctgc tcgaggcccg cgagcggggc
     4501 ctcgagccgg gcgatcgcct cccgaccgag gccgacctcg agtcgcgcta cggcgtgtcg
     4561 cgaacgacga tccggcaggc cctgagcgag ctggaggcgg aagggctggt ccgccgggtc
     4621 caggggaagg gcacgttcct cggaaccccg cggatccagc acgtcccggt gctcgcctcg
     4681 ttctccgagc tcctgcgcag ccagggcttc gacccgtccc accggctcct ggcctccgcc
     4741 gtccggccgg cgttgccgga ggtggcggag gggctcgggc tggaggccgg cgccgagtgc
     4801 cgcttcctgc ggcggctgtt cctggccgac ggggacccgg tggggctggc ggagacctgg
     4861 cttccccgcg cgctgctcgg cccgagcgac gccctcttcc agaagggcga gatcgaggac
     4921 ggatcgctct acgagctcct ccagcgcccg ccgatcggcc tcgtgctgca ccgcgcgatg
     4981 gagacgatca gcccggacgt ggccgccgcg gccgacgcgc gccggctgcg ctgcccgccg
     5041 ggcagccccc tgctggtgat cgtgcgcgtg acgttcacgc ccgacgaccg tcccgtggag
     5101 tacacccggc tgctgttcgc cggcgaccgc tacgagtacc gcgtggagat gcagcgcccg
     5161 gccgagatcc ggaggcgatg aggtggagac gcgtgatgtg cgcgaggtga ttgccgccgt
     5221 ggttggcggc ctggtcggcg aggggaacgg cgcgcccgtt ggcggtccgg ataacgtccc
     5281 tgctgccgac ggcgagcccg ctcccccgcg ccgccccgcc ggcggcctgg ccgagatcgc
     5341 cgtcgggtcc gaccacgccg ggttcctcct gaagcagcgc atcgccgccg acctgctcga
     5401 gcacggcttc accgtgcacg attgcgggcc cgaggcgccg gacccggtcg actaccccga
     5461 ctacgcgcac gccgtcgccc ggctggtggc cgacggggac tgccgctggg ggatcgtgat
     5521 cgatggggcc ggcatcggtt cggccatcgc g
//
LOCUS       DASPWO010000432         5769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_993275, whole genome shotgun sequence.
ACCESSION   DASPWO010000432 DASPWO010000000
VERSION     DASPWO010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993275"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..340)
                     /locus_tag="VEO00_09805"
     CDS             complement(23..340)
                     /locus_tag="VEO00_09805"
                     /inference="COORDINATES: protein motif:HMM:NF017426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HYU58329.1"
                     /translation="MRISATPAAEDTVRQVARSGRTDLVMVLGTGCCDSTAPFLYDRY
                     EPGADAVRIGEVAGVPVFAHAWLARLYADRAALQVDVDKDLVNDSFSLESELDCRFTL
                     RVT"
     gene            complement(370..1872)
                     /locus_tag="VEO00_09810"
     CDS             complement(370..1872)
                     /locus_tag="VEO00_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005168983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HYU58330.1"
                     /translation="MAREFKLLIDGEFVDAASGETSESRSPSDGELVATVAKGGAEDV
                     NRAVSAARRAFDEGPWPSMKPAERWKIMRRVHEDLEERSAELAMLEAEDAGHTARMAH
                     LFTVPYANEFWRYLGELGGKMSFFEPVDQYDFPAPAWHHVRREPVGVVGAITPWNYPY
                     LMAMWKLSPALATGCTIVLKPASYTPSTTMALAEIIAESGIPPGVVNVVAGPGAAVGE
                     TLVTHPDVNKISFTGSTEIGRRIMQLGAGTVKRLTLELGGKSAAIVLDDADLEDAVPG
                     AIWAMFMHCGQSCEAATRLLLPASLHDEFLAMMVERVEGLSIGPATDFESDLGPLINE
                     TQLQTVERYVQLGQDEGAKLLTGGERATGEGLENGAYYRPTIFADVDNAMTIAQEEIF
                     GPVLSVIRYEGVDQAIRIANDSIYGLGGSVWSSDPIRAVEVAKRVKTGSVWINAHHIL
                     TPAAPFGGYKQSGFGREFGPWGLDEYLETKYIRVEQLAPKDKIWNQVIGA"
     gene            2012..2608
                     /locus_tag="VEO00_09815"
     CDS             2012..2608
                     /locus_tag="VEO00_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58331.1"
                     /translation="MSSRRFAALLVALAFAAAGCRSDSPTIVVPSETGTPSPTGGSPS
                     PTAAPTLAGASCEPTTGGSSSNTPHFVDVDTARSNGVESITFEFAAATSGEAPSSGVE
                     YVDELTGDPSGEPVEIEGEAFLQVHFGAIATDLSGETPVQVYAGPRDIPIGFPVLRQA
                     KLIGDFEAVVTWGIGLSSRQCFVAEADADSLTISVASA"
     gene            complement(2620..4236)
                     /locus_tag="VEO00_09820"
     CDS             complement(2620..4236)
                     /locus_tag="VEO00_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58332.1"
                     /translation="MNDLRSHLAQLERTPPPDLWPDIETRRPRRRVPDPDLPRRILAA
                     FVALALAAGGLIVAVRAFTGTKEVAPAQEVTGRAEDLPLECTVRLLTPEVEPGGPVET
                     EFVYRNVGESDLQVATGPARLLVDDRAGTAVYDTDFSGYLTGGNDFARPFPLPAGQAV
                     RWPSVFHSLWGGTIALHPRCEYSVLDADKRREAEGVIEPGPLHVTVASSGGSPNREDA
                     LALALEPTGHLFDRCAVTADGRPVRGWVDPPDGDLGVPSQEATCRATVRQEPGFAVVD
                     LMFASPPDVPLPEPSESRTRGLALPGQAVGTITRWTFLVRDGAAREIVPGPLEDLDPE
                     LVRVSSLSTAPDRCAFEDRDLACSEILYVPTADGWASGEIRERDPEPASRDGIVFFAP
                     SPDVPLEPPTTLASGSFDGKAWSFEIFRAVGGLRGRAILEDQPPERVITLAAPSPAEI
                     CGARLIAGILGDTRQGLLISVTASPRVVRMDLIAGDQVTAMSLVSIPPGYDAPFSFWL
                     FVGENPPPGNQPPELAAYDRNGIRIDHGEC"
     gene            complement(4233..4700)
                     /locus_tag="VEO00_09825"
     CDS             complement(4233..4700)
                     /locus_tag="VEO00_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU58333.1"
                     /translation="MRELSVTPRTDLERLYREQGDRLWWALLGYSGDPEVASDAVAEA
                     FAQALRGGERIREPLAWIWRVAFRVAAGELKRRRRESGGAREIAYELDRTGAAIVEAL
                     QRLSPRQRAAILLHHYAGYPVKEVAAILGSSPTTVSVHLHRARKRLAELLEAS"
     gene            4829..5620
                     /gene="panB"
                     /locus_tag="VEO00_09830"
     CDS             4829..5620
                     /gene="panB"
                     /locus_tag="VEO00_09830"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HYU58334.1"
                     /translation="MTVTVQDLRAWKSQGRRFAMLTAYDYPTAQILDEAGVPVLLVGD
                     SVANNILGYDTTLPVTMEEMLHHARAVARGAKNALLVGDMPFGSYQVSTEDALENAAR
                     YLKEGGMHAVKLEGAMLDLAQALVERGIPVMGHLGLTPQSVHAMGGYRVQARTEEAAE
                     RLLNDALNLEKSGVFSLVLEGIPSAVAKRVTASLQVPTIGIGAGPDCDGQVLVITDLL
                     GLGGGKYPKFVKPYANLRQTITDAVRAYVDDVESGRFPDAEHSYS"
BASE COUNT          860 a         2046 c         2035 g          828 t
ORIGIN      
        1 ggctcgctcc tcggccactc ggtcaggtga ctcggagcgt gaagcggcag tcgagctcgc
       61 tctcgagtga gaacgagtcg ttcacgaggt ctttgtccac gtccacctgg agggcggcgc
      121 ggtcggcgta gagccgggcg agccatgcgt gggcgaagac cgggaccccg gccacctcgc
      181 cgatccgcac ggcgtccgcc cccggctcgt agcggtcgta gaggaagggc gcggtggagt
      241 cgcagcagcc ggtgccgagc accatcacca ggtccgtccg gcccgagcgg gcgacctgcc
      301 ggacggtgtc ctcggcggcc ggggtggcgc tgatcctcac gaccggagcg taccccgcgg
      361 tccggccgtt caggcgccga tgacctggtt ccagatcttg tccttcgggg cgagctgctc
      421 gacccggatg tacttcgtct ccaggtactc gtccagcccc cacgggccga actcccggcc
      481 gaaccccgac tgcttgtagc cgccgaacgg cgcggccggc gtcagtatgt ggtgcgcgtt
      541 gatccagacc gaaccggtct tcacccgctt ggccacctcc actgcccgga tcgggtccga
      601 ggaccacacc gagccgccca gcccgtagat cgagtcgttc gcgatccgga tggcctggtc
      661 gacgccctcg tagcggatca ccgagaggac cggcccgaag atctcctcct gggcgatggt
      721 catggcgttg tccacgtcgg cgaagatcgt cggccggtag tacgcgccgt tctccaggcc
      781 ttcgccggtg gcgcgctcgc cgccggtcag cagcttcgcc ccctcgtcct gcccgagctg
      841 cacgtagcgc tccacggtct gcagctgcgt ctcgttgatc agcggcccga ggtcgctctc
      901 gaagtcggtg gccgggccga tcgacaggcc ctcgacgcgc tcgaccatca tcgccaggaa
      961 ctcgtcgtgc agggacgccg gcagcagcag gcgggtcgcc gcctcgcagg actggccgca
     1021 gtgcatgaac atcgcccaga tcgcgccggg gacggcgtcc tccaggtcgg cgtcgtcgag
     1081 gacgatcgcc gcgctcttgc cacccagctc gagcgtcagc cgcttcacgg tgccggcgcc
     1141 gagctgcatg atccgccggc cgatctccgt ggagcccgtg aacgagatct tgttcacgtc
     1201 ggggtgggtg acgagcgtct cgccgacggc cgccccgggc ccggccacga cgttcaccac
     1261 gccgggcggg atgcccgact cggcgatgat ctcggcgagg gccatcgtgg tcgacggggt
     1321 gtagctcgcc ggcttgagga cgatcgtgca gccggtggcg agggcggggg agagcttcca
     1381 catcgccatc aggtacgggt agttccaggg cgtgatcgcc ccgaccacgc cgaccggctc
     1441 gcgccggacg tggtgccagg ccggcgcggg gaagtcgtac tggtcgacgg gctcgaagaa
     1501 gctcatcttg ccgccgagct cgccgaggta gcgccagaac tcgttcgcgt agggcaccgt
     1561 gaacaggtgg gccatccgcg cggtgtgccc ggcgtcctcg gcctcgagca tcgccagctc
     1621 ggcggagcgc tcctcgaggt cctcgtgcac gcgccgcatg atcttccagc gctcggccgg
     1681 cttcatcgac ggccacggtc cctcgtcgaa ggcgcgccgc gccgccgaca ctgcccggtt
     1741 gacgtcctcc gcgccgccct tggcgaccgt cgccacgagc tcgccgtcgg acgggctgcg
     1801 ggactcggac gtctccccgc tcgccgcgtc cacgaactcg ccgtcgatca ggagcttgaa
     1861 ctcgcgtgcc atggccgact cctcgcgcct cgggccgatc gggatcccgg cgtcctctgg
     1921 gcggccggga gcccaggata cgccactgac caccggtcag agaagctggg cccggcctgc
     1981 ggcgcggagg ccgggcgtaa gctgtggcgc gatgtcgtcc cggcggttcg ccgcgctgct
     2041 cgtggcgctc gcgttcgcgg cggcagggtg ccgcagcgac tcgccgacga tcgtggtgcc
     2101 gtccgagacc ggcacgccga gcccgacagg cggatcgccg tcgccgaccg cggcgcccac
     2161 actcgccggc gcgtcctgcg agccgacgac cggcggctcg tcctcgaaca ccccgcactt
     2221 cgtggacgtg gacacggcgc gatcgaacgg cgtcgagtcg atcacgttcg agttcgccgc
     2281 cgccacctcc ggcgaggccc cgtcgtccgg cgtggagtac gtcgacgagc tgaccggcga
     2341 cccgtcgggg gagccggtcg agatcgaggg cgaggcattc ctgcaggtcc acttcggtgc
     2401 catcgccacg gacctgtccg gcgagacgcc cgtccaggtc tacgccggcc cgcgcgacat
     2461 cccgatcggc ttcccggtcc tgcgccaggc gaagctgatc ggcgacttcg aagccgtcgt
     2521 cacctggggc atcggcctct cgagccgaca atgcttcgtc gccgaagccg acgccgacag
     2581 cctgaccatc agcgtcgctt ccgcctgaaa gacggctctt cagcactccc cgtggtcaat
     2641 gcggatgccg ttgcggtcgt acgcggcaag ttccggcggc tggttccccg gtggagggtt
     2701 ctccccaacg aacagccaga aggagaaggg ggcatcgtag ccaggtggga tcgagacgag
     2761 tgacatcgcg gtgacctggt caccagcgat cagatccatc cgcacgacgc gcggcgatgc
     2821 cgtcaccgag atgagcaatc cctgtcgagt gtcgccgagg atcccggcga tgagcctggc
     2881 gccacagatt tcagcagggg acggcgcggc aagtgtgatc actcgttccg gaggctgatc
     2941 ttcgaggatc gcccttccgc gcagaccccc gacggcgcgg aagatctcga acgaccacgc
     3001 cttgccgtcg aacgagcccg acgccagggt cgtgggcggc tccagaggca cgtccgggga
     3061 gggagcgaag aacacgatgc cgtcgcgaga tgcgggctcc ggatcccgct cgcgaatctc
     3121 gccactggcc catccgtccg cagtcggcac gtagaggatc tcggagcatg ccaagtcccg
     3181 gtcctcgaac gcgcatcggt cgggagccgt cgagaggctg ctcacacgga cgagttccgg
     3241 atccaggtcc tcgagcgggc cggggacgat ttcgcgtgcc gcaccatcgc ggaccaggaa
     3301 ggtccatcgc gtgatcgtcc cgacggcctg cccgggcagg gcgaggccgc gggttcggct
     3361 ttccgaaggc tctggcaagg gaacgtctgg cggcgaggcg aacatgaggt cgacgacagc
     3421 gaagcccggc tcctgccgga cggtcgcgcg gcacgtggct tcctgcgacg ggacgccaag
     3481 gtcaccatcg gggggatcga cccaccctcg taccggccgg ccatcggccg tgacggcgca
     3541 ccgatcgaac aggtggcccg tcggctcgag agcgagggcc agagcatcct cgcggttcgg
     3601 tgatcctcca gaggaagcca cggtcacgtg cagcggtccc ggttcgatca cgccctctgc
     3661 ctcccggcgc ttgtccgcat caaggaccga gtactcgcat cgcggatgga gtgcgatggt
     3721 gcctccccag agggagtgga agacgctcgg ccaccgcacg gcctgccctg ccggcaaggg
     3781 gaaggggcga gcgaagtcgt tgcctccggt gaggtaaccg ctgaagtccg tgtcgtatac
     3841 ggcggttcct gcccggtcgt cgacgagaag tctcgccggc ccggtggcca cctggagatc
     3901 gctctcgccg acattgcgat agacgaactc ggtctccacc gggccccccg gctcgacctc
     3961 gggggtgagc aggcggacgg tgcactcgag cgggaggtcc tcggcacggc cggtgacctc
     4021 ctgggcgggg gcgacctcct tcgtcccggt gaacgcacgg accgcgacga tcaggcctcc
     4081 cgcggccagg gcgagggcga cgaaggcggc caggattcgg cgggggaggt cggggtcggg
     4141 gacgcggcgg cgcgggcggc gggtctcgat gtcgggccac aggtcgggcg ggggggtccg
     4201 ctcgagctga gccaagtgcg atcgcaggtc gttcatgacg cctccagcag ctctgccagt
     4261 cgtttccgtg cccggtggag atggacggag acggtcgtgg gggacgaacc gaggatggcg
     4321 gcgacctcct tcaccgggta gcccgcgtag tggtgcagca ggatcgccgc gcgctggcgc
     4381 ggcgagagcc gctgcagcgc ctcgacgatc gccgcgcccg tccggtcgag ctcgtaggcg
     4441 atctcgcggg cgccgcccga ctcgcgccgc cgccgcttca gctcgccggc ggccacgcgg
     4501 aacgccaccc gccagatcca tgccagtggc tcgcggatgc gctcgccgcc ccgcagcgcc
     4561 tgggcgaacg cctcggccac cgcgtcgctc gccacctcgg ggtccccgct gtagccgagc
     4621 agcgcccacc acagccggtc gccctgctcg cggtacaggc gttccagatc cgtccgaggg
     4681 gtcacgctca gctcacgcat ccgtccccct caaggcgccg ccggaccccg gaacctttac
     4741 acgccacagc cgggcagggg aactcgcggc ggacggcgta cgattaccgg tgacacggac
     4801 gccgtgcact cggaaggagg cgcgagccat gaccgtcacc gtccaggacc tccgggcctg
     4861 gaaatcgcag ggccgtcgct tcgcgatgct gaccgcgtac gactacccga ccgcacagat
     4921 cctcgacgag gccggggtac ccgtgctgct cgtcggcgac tcggtcgcca acaacatcct
     4981 cgggtacgac accaccctcc cggtcacgat ggaggagatg cttcaccacg cccgggccgt
     5041 cgcccggggc gcgaagaacg cgctgctggt cggcgacatg ccgttcggct cctaccaggt
     5101 ctccaccgag gatgccctcg agaacgccgc ccggtacctg aaggagggcg gcatgcacgc
     5161 ggtcaagctc gagggcgcga tgctcgacct ggcgcaggcg ctcgtcgagc ggggcatccc
     5221 ggtgatgggc catctcgggc tgaccccgca atcggtccac gccatgggcg gctaccgggt
     5281 ccaggcccgt accgaggagg ccgccgagcg gctgctgaac gacgcgctca acctggagaa
     5341 gtccggagtg ttctcgctgg tgctggaggg gatcccgtcg gcggtggcca agcgcgtcac
     5401 cgcgagcctg caggtgccga cgatcgggat cggcgccggc ccggactgcg acggccaggt
     5461 gctggtgatc accgacctcc tcggcctggg cggcgggaag tacccgaagt tcgtcaagcc
     5521 ctacgcgaac ctccgccaga cgatcaccga cgccgtgcgc gcctacgtgg acgacgtcga
     5581 gtccggccgc ttccccgacg cggagcattc ctactcgtag gacccgggcg cgaaccgctc
     5641 gagggggccg gatccggcgg cccacgacga tccgcgcgga tcgcagccgc gacggacacg
     5701 gactgaacag gccgttcggc cctggggccc ggctgctggg agggctacgg tgcagcgaga
     5761 caggcacca
//
LOCUS       DASPWO010000433         3866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1163727, whole genome shotgun sequence.
ACCESSION   DASPWO010000433 DASPWO010000000
VERSION     DASPWO010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163727"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VEO00_09835"
     CDS             <1..260
                     /locus_tag="VEO00_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HYU58335.1"
                     /translation="RIDLQALQARVPYDLYPVVLRLATQEPPQAAGIPEPVPQEPLDE
                     GPHLSYAVQWFAFAAIGLIGWPILLRRAARDRGDTRDRPTD"
     gene            complement(270..464)
                     /locus_tag="VEO00_09840"
     CDS             complement(270..464)
                     /locus_tag="VEO00_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58336.1"
                     /translation="MTISGVYRTERGTIWRLVHLEGGSMRVELLTNETWVPGPIAMAG
                     LRLSIQCKELTAAAIRKLPP"
     gene            complement(469..678)
                     /locus_tag="VEO00_09845"
     CDS             complement(469..678)
                     /locus_tag="VEO00_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013283833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYU58337.1"
                     /translation="MTTGSVKWFSPEKGFGFITPDDGSADVFVHFSAITGEGYRNLEE
                     NQKVEFDVSQGPKGPQASNVRPVMA"
     gene            complement(1005..1196)
                     /locus_tag="VEO00_09850"
     CDS             complement(1005..1196)
                     /locus_tag="VEO00_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58338.1"
                     /translation="MKRTGGSSALPKGGSDRPIRSYGEDRTCSICDTRLSRYNESGTC
                     SVHRGWGAGPNARPSVPKG"
     gene            complement(1250..1741)
                     /locus_tag="VEO00_09855"
     CDS             complement(1250..1741)
                     /locus_tag="VEO00_09855"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HYU58339.1"
                     /translation="MEAPKRIKPKGLADYLEVITKAVFQSGISWRVIEAKWDGFREAF
                     ANFDPQTIAGFDPPDVDRLAEDTRIVRNRKKIEATVHNARTLLELDTEFGSFKRYLRS
                     FGSYDDTVADLRRRLKFLGDTGAYFFLYVVGEEVPAHEDWMAARGGQMGRPRSRARGG
                     RTA"
     gene            complement(1858..2100)
                     /locus_tag="VEO00_09860"
     CDS             complement(1858..2100)
                     /locus_tag="VEO00_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HYU58340.1"
                     /translation="MSGYVLIEADVGRAAEVTTFAARLAGVRSADEITGPYDVIVSAV
                     EMDPTRFLRLVEEIERLAGVTRTIPCPAGARMPVAA"
     gene            complement(2115..3347)
                     /locus_tag="VEO00_09865"
     CDS             complement(2115..3347)
                     /locus_tag="VEO00_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HYU58341.1"
                     /translation="MSEFEAVRGFFEDAAARLNLEPSLREVLSRPQRELHVQVRVPMD
                     DGSLKVFPGYRVQYNDARGPFKGGIRFHPLVNIDEIRTFAALMTWKTALMNIPFGGGK
                     GGVQVDPKALSEAELERLSRAYLRAIADIIGPQVDVPAPDVNTTPLIMAWMADEYMRE
                     RGRAPAVITGKPIVVGGSLGRDAATGRGAAICLDHYVKSRGWKREDISVAVQGYGNAG
                     SWLAILLEEMGYPVVAVSDSRGGIIFDRGLPTREVLEHKRATGSVIGFPGGETVDGQE
                     LLGLGSTVLALAALDESVTEENAIDVKAKTILEVANYPVTPEASEIIGDRGGAILPDI
                     LASGGGVAVSYLEWVQDLQGESWPEERVNARLEELIGPATDQVLRRAEFDGVSPRQAA
                     YLIGVGRVAEAETARGYR"
     gene            3456..>3866
                     /locus_tag="VEO00_09870"
     CDS             3456..>3866
                     /locus_tag="VEO00_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU58342.1"
                     /translation="MSVCKVCGEENPDRAKFCLNCASPLGAAEATEAEERKVVSVLFA
                     DLVGFTARSEEADPEDVRAILRPYHAALKREIERYGGTVEKFIGDAAMGVFGAPLAHE
                     DDPERAVRAALRIQEAIAELNEATPRLALSVRAAV"
BASE COUNT          511 a         1457 c         1281 g          617 t
ORIGIN      
        1 cccggatcga cctgcaggcg cttcaggcgc gggtgccgta cgacctctac ccggtggtgc
       61 tcaggctggc gacgcaggaa ccgccgcagg cggcggggat ccccgaaccg gtcccgcagg
      121 agccgctcga cgaggggccg cacctgtcgt acgcggtgca gtggttcgcg ttcgcggcga
      181 tcgggctgat cggctggccg atcctgctgc gccgagccgc ccgcgaccgc ggggacacga
      241 gagaccgacc gacagattga ccgacggcgc tacggcggga gcttgcggat ggccgcggcc
      301 gtgagttcct tgcactggat cgagagacgg agcccggcca tggcgatcgg gccgggcacc
      361 cacgtctcgt tcgtcaggag ttcgacgcgc atcgagccgc cttcgagatg cacgagtcgc
      421 cagatcgtgc cgcgttcggt gcggtagacc cccgaaatgg tcatgtggtt aggccatgac
      481 cggcctgacg ttcgatgcct gggggccctt ggggccctgg ctgacgtcga actcgacctt
      541 ctggttctcc tcgaggttgc ggtagccctc gcccgtgatc gcgctgaagt gcacgaagac
      601 gtcggcgctg ccgtcgtcgg gggtgatgaa gccgaacccc ttctcggggc tgaaccactt
      661 gactgagccc gtggtcatga agcattccct cctttcccgg cctggccgga cctgcgtgct
      721 gtccggcggc ctccggcgcg gctgcgccgg gcgagggaac tgctccgaag ctggatcatg
      781 ctcaaactgc gatgctgaac cctcaaccca ggcattgtag cccttccgag ggttccctga
      841 ctcggccccg ccgggtcagc gcccggcggc cttcttgacc ttcttcgctt ggcgcttctc
      901 tttcaggctc ttctgggcgg tcttcttgct gctcttgctg ccgcccttgt ccttgttggc
      961 cacggcgcct ccttccgtgc tcggctcgcg ccgggcgtcc gcgctcagcc cttcggcacg
     1021 ctgggccggg cgttcggccc ggcgccccag ccgcggtgca cgctgcaggt cccgctctcg
     1081 ttgtagcggg acagccgggt gtcgcagatc gaacaggtcc ggtcctcgcc gtaggaacgg
     1141 atcggccggt cactgccgcc cttcggcaac gcggaactac caccggttcg cttcatctgc
     1201 cgtcttcccc ctcccgagcg cccggaactc gggagccgta ccgcacagcc tacgcggtac
     1261 ggccgcccct cgcccgcgat cgcggccggc ccatctgacc gccgcgcgcg gccatccagt
     1321 cctcgtgcgc cgggacctcc tcgccgacga cgtagaggaa gaagtaagcg ccggtgtcgc
     1381 cgaggaactt caggcgccgg cgcaggtcgg ccaccgtgtc gtcgtacgag ccgaacgagc
     1441 gcaggtacct cttgaagctc ccgaactccg tgtcgagctc gaggagcgtc cgcgcgttgt
     1501 gcacggtggc ttcgatcttc ttgcggttgc ggacgatgcg cgtgtcctcc gccagccgat
     1561 ccacgtccgg cgggtcgaac ccggcgatgg tctgcgggtc gaagttcgcg aaggcctccc
     1621 ggaacccgtc ccacttcgcc tcgatcaccc gccagctgat cccggactgg aacaccgctt
     1681 tggtgatcac ctcgaggtag tccgccagcc ccttcggctt gatccgcttc ggcgcctcca
     1741 cccgaacctc cttgtccctc cccggaggcc ccatcatgcg ccaatgcagg cccccgggtt
     1801 ccacgggcgc cctgccgtgg aacccgggag ccaccgccgc cgcggtcgtc ctctggctca
     1861 ggccgcgacc ggcatcctgg cgccggccgg gcaggggatc gtccgggtca ccccggccaa
     1921 tcgctcgatc tcctcgacga gccgcaggaa ccgcgtcggg tccatctcga cggccgacac
     1981 gatcacgtcg tagggcccgg tgatctcgtc ggccgaccgc acgccggcca gccgcgcggc
     2041 gaaggtcgtc acctccgccg ccctgccgac gtcggcctcg atcaagacgt agccgctcat
     2101 ccgcgcaccc cccgctatcg gtatccgcgg gcggtctcgg cctcggccac ccggcccacg
     2161 ccgatcaggt aggccgcctg gcgcggcgac acgccatcga actccgcccg ccgcagcacc
     2221 tggtcggtgg ccggcccgat cagctcctcg agccgggcgt tgacccgctc ctcgggccac
     2281 gactcgccct gcaggtcctg cacccactcc aggtacgaga cggccacgcc gcccccggag
     2341 gccaggatgt ccggcaggat cgcgccgccc cggtcgccga tgatctcgct cgcctcgggc
     2401 gtcaccgggt agttcgccac ctcgaggatc gtcttggcct tcacgtcgat ggcgttctcc
     2461 tcggtgacgc tctcgtcgag cgccgccagc gccagcaccg tgctgcccag cccgagcagc
     2521 tcctggccgt cgacggtctc gccgccgggg aacccgatca cgctgccggt ggcccgcttg
     2581 tgctccagca cctcgcgggt cggcaggccg cggtcgaaga tgatgccgcc cctgctgtcg
     2641 gagaccgcca ccaccgggta gcccatctcc tccagcagga tcgccagcca cgacccggcg
     2701 ttcccgtagc cctgcaccgc caccgagatg tcctcgcgct tccagccgcg cgacttcacg
     2761 tagtggtcca ggcagatcgc cgcgccgcgc ccggtcgccg cgtcgcgccc gagcgagccg
     2821 cccaccacga tcggcttgcc cgtgatcacc gccggggccc ggccgcgctc gcgcatgtac
     2881 tcgtcggcca tccaggccat gatgagcggc gtcgtgttca cgtcgggcgc cggcacgtcg
     2941 acctgcggcc cgatgatgtc ggcgatcgca cgcaggtagg cgcgggacag ccgctcgagc
     3001 tccgcctcgg acagcgcctt cgggtcgacc tgcacgccgc ccttgccgcc gccgaacggg
     3061 atgttcatca gcgccgtctt ccacgtcatc agcgccgcga acgtccggat ctcgtcgatg
     3121 ttcaccagcg ggtggaaccg gatgccgccc ttgaacggcc cgcgggcgtc gttgtattgc
     3181 acccggtagc ccgggaagac cttcaggctc ccgtcgtcca tcggcacccg gacctggacg
     3241 tggagctcgc gctggggcct ggagagcacc tcgcgcagcg agggctcgag gttcagccgc
     3301 gcggccgcat cctcgaagaa cccccgtacc gcctcgaact cggacatggc acacctcctc
     3361 gtgtcaggcc gagtgtcgat cggggggggt ccggccggac agggcccttc gtcccctgcg
     3421 ggagggggaa ctgtggcccc gtagactgcg ttgacgtgtc cgtctgcaag gtctgcggcg
     3481 aggagaaccc ggaccgggcg aagttctgcc tgaactgcgc cagcccgctc ggcgccgccg
     3541 aggcgaccga ggccgaggag cgcaaggtgg tctcggtcct gttcgccgac ctggtcggct
     3601 tcaccgcgcg gtccgaggag gccgatcccg aggacgtccg ggcgatcctc cgtccgtacc
     3661 acgccgcgct gaagcgcgag atcgagcgct acggcggcac cgtcgagaag ttcatcggcg
     3721 acgccgcgat gggcgtgttc ggcgcgcccc tggcccacga ggacgacccc gagcgggcgg
     3781 ttcgcgcggc gctgcgcatt caggaggcca tcgccgagct gaacgaggcc acgccccggc
     3841 tggcgctctc cgtccgggcc gccgtg
//
LOCUS       DASPWO010000434         3175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1163830, whole genome shotgun sequence.
ACCESSION   DASPWO010000434 DASPWO010000000
VERSION     DASPWO010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163830"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VEO00_09875"
     CDS             <1..244
                     /locus_tag="VEO00_09875"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636406.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HYU58343.1"
                     /translation="ADFVKTSTGFGPGGATVHDVLLMRETVGPEMGGKAAGGVRTVED
                     VRDMIAAGATRIGASAGVQIVSGGRSDGPPWRGGDR"
     gene            241..876
                     /locus_tag="VEO00_09880"
     CDS             241..876
                     /locus_tag="VEO00_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58344.1"
                     /translation="MTNGIELRAYCFLDSLQPQYAAFLGTVAQGFLPLAGDASLYVEI
                     SPGIEINRLTDVALKQTNVTPGMQIIERYYGMLEVHSADQADTRAAGAAILDDAGLKE
                     DDRIKPRILASQIVRRITGHHAQLLNRGRHGEMILAGQTLYVLECEPAAYAALAANEA
                     EKAAEINVLEVRSFGSNGRIYLGGEERDIDVGWQAAVAALEGLSGREGTSR"
     gene            892..1215
                     /locus_tag="VEO00_09885"
     CDS             892..1215
                     /locus_tag="VEO00_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HYU58345.1"
                     /translation="MAEALGMIECRSFPAMVEAADAMVKAAKVELVSYAETGGGYVTA
                     VVRGDVAAVKAAVQAGVQNSERVGEMVASHVIARPHVNIDLVLPLGRREEAESEMGRG
                     EAAAQ"
     gene            1282..1584
                     /locus_tag="VEO00_09890"
     CDS             1282..1584
                     /locus_tag="VEO00_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HYU58346.1"
                     /translation="MLLGRVAGTLVSTRKEPSMEGLKFLVVRTLDPENAETGQFVVAA
                     DTVGAGEGEVVLVAQGSSARQTEATRDRPCDAVIMAIVDTWEVGGNERYHKYRGER"
     gene            1581..3038
                     /locus_tag="VEO00_09895"
     CDS             1581..3038
                     /locus_tag="VEO00_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYU58347.1"
                     /translation="MKTLGQDDIQAIIDRVRRRLGEAGEHPGVALRGEEELSRAQPEL
                     GDGIFATIGECVDAASRAFGAYRSVGLEGRKRIIAAVRASMLENAERLAEMARAETGL
                     GRAEDKVRKNRLVASKTPGPEDLELEAVTGDLGMTVTEFAPFGVVASITPTTNPTSTV
                     INNTISIVSAGNAAVFNVHPNAKRVSAENVRLINRAVVSAGGPEHLVSAIAEPTIESA
                     KELMHHPDVRLLLVTGGPGVVKEALKTDKRAVTAGPGNPPAVVDQTADVEKAARDIVA
                     GASFDNNVICTDEKTAIVVDSVGDRLVRAMGQHGGHVLKEHELRRLERVIFKELGAPN
                     RPGVIDPRWIGKDAGVILAEIGVRAGPEVRLVVAEVPREDSLVWTEQMMPVMPVVRVA
                     DVEQAIDLAVRSEHGFRHTASIHSTNVDAITRMGRAMNCSIFVANGPNYAGLGEGGEG
                     FCSFSIASPTGDGLTRPRTFSRERRITVVGSLRIV"
BASE COUNT          490 a         1072 c         1211 g          402 t
ORIGIN      
        1 cgccgacttc gtcaagacct ccaccgggtt cggccccggc ggcgccaccg tgcacgacgt
       61 cctgctgatg cgggaaacgg tcgggcctga gatggggggc aaggctgccg gaggggtgcg
      121 gaccgtggag gacgtgcggg acatgatcgc cgccggcgcc acccggatcg gcgcgtctgc
      181 cggggtgcag atcgtgagcg gggggcgcag tgatggcccc ccgtggagag gaggcgaccg
      241 gtgaccaacg gcatcgagct ccgggcgtac tgcttcctgg acagcctcca gccgcagtac
      301 gccgcgttcc tgggcacggt ggcccagggg ttcctgcccc tggccggcga tgcctcgctg
      361 tacgtcgaga tctctccggg gatcgagatc aaccggctga ccgacgtggc gctgaagcag
      421 acgaacgtca cgccgggcat gcagatcatc gagcgctact acgggatgct cgaggtccac
      481 tcggccgatc aggccgacac ccgcgcggcc ggcgcggcca tcctcgacga cgccgggctg
      541 aaggaggacg accgcatcaa gccgcggatc ctggccagcc agatcgtccg ccggatcacc
      601 gggcatcacg cgcagctgct caaccggggg cggcacgggg agatgatcct cgccggccag
      661 accctctacg tcctggaatg cgagccggcg gcctacgcgg cgctggccgc gaacgaggcc
      721 gagaaggccg cggagatcaa cgtcctcgag gtccgttcgt tcggctcgaa cggccggatc
      781 tacctcgggg gcgaggagcg cgacatcgac gtcggctggc aggccgcggt ggccgcgctc
      841 gagggattgt cgggccgcga aggcacgagc cggtaggagg aggagggagt catggcagag
      901 gcccttggga tgatcgagtg ccggagcttc ccggccatgg tcgaggccgc cgacgcgatg
      961 gtgaaggcgg ccaaggtcga gctggtgtcc tacgcggaga ccggcggcgg ctacgtgacc
     1021 gccgtcgtgc ggggcgacgt tgccgcggtg aaggcggccg tccaggccgg cgtccagaac
     1081 tccgagcggg tcggcgagat ggtcgcgagc cacgtcatcg cccggccaca cgtgaacatc
     1141 gacctggtgc tgccgctcgg ccgccgcgag gaggccgagt cggagatggg ccggggcgag
     1201 gcggcggccc agtaggagcg gacccggtac ggggcgccgg gccgtccggt ccggcgcccc
     1261 tgcgggggga aggaggcgga cgtgctcctg ggacgcgtgg ccgggacgct ggtctccacc
     1321 cggaaggagc cctccatgga gggcctgaag ttcctggtgg tgcgaacgct ggaccccgag
     1381 aacgccgaga ccggccagtt cgtggtggcg gccgacaccg tcggcgccgg ggagggtgag
     1441 gtggtcctgg tcgcccaggg cagctccgcg cgccagaccg aggccacccg cgaccggccg
     1501 tgcgacgcgg tgatcatggc catcgtcgac acctgggagg tcggcggcaa cgagcgctac
     1561 cacaagtacc ggggtgagcg atgaagacgc tcgggcagga cgacatccag gcgatcatcg
     1621 accgggtccg gcggcggctg ggagaggccg gcgagcatcc cggtgtggcg ctgcgcggcg
     1681 aggaggagct gtcccgcgcg cagccggagc tcggggacgg catcttcgcc acgatcggcg
     1741 agtgcgtcga cgcggcctcg cgcgcgttcg gagcgtatcg aagcgtcggg ctcgagggcc
     1801 gcaagcggat catcgccgcc gtgcgggcgt cgatgctcga gaacgccgag cgcctggcgg
     1861 agatggcccg cgccgagacc gggctcgggc gggccgagga caaggtgcgc aagaaccggc
     1921 tggtggcctc gaagaccccc ggcccggagg acctcgagct cgaggccgtg accggcgacc
     1981 tcgggatgac ggtcaccgag ttcgcgccgt tcggcgtcgt cgcgtcgatc accccgacga
     2041 cgaaccccac ctcgaccgtg atcaacaaca cgatctcgat cgtctccgcc gggaacgcgg
     2101 cggtgttcaa cgtccacccg aacgcgaagc gcgtgtcggc cgagaacgtc cggctgatca
     2161 accgggcggt cgtgtccgcc ggcggccccg agcacctcgt ctccgcgatc gccgagccga
     2221 cgatcgagag cgccaaggag ctgatgcacc accccgacgt ccggctgctg ctcgtcaccg
     2281 gcgggccggg cgtggtcaag gaggccctga agaccgacaa gcgcgcggtg acggccggcc
     2341 ccgggaaccc gccggcggtc gtcgaccaga cggccgacgt cgagaaggcc gcccgggaca
     2401 tcgtcgccgg cgcctccttc gacaacaacg tgatctgcac cgacgagaag acggcgatcg
     2461 tcgtcgactc cgtcggcgac cggctggtgc gggcgatggg ccagcacggc ggccacgtcc
     2521 tgaaggagca cgagctgcgc cggctggagc gcgtgatctt caaggagctc ggcgctccga
     2581 acaggcccgg cgtgatcgac ccgcgctgga tcggcaagga cgccggcgtg atcctcgccg
     2641 agatcggcgt ccgcgccggg ccggaggtcc gcctggtggt ggccgaggtg ccgcgcgagg
     2701 acagcctggt gtggaccgag cagatgatgc ccgtgatgcc ggtggtccgc gtcgccgacg
     2761 tggagcaggc catcgacctc gccgtgcgct cggagcacgg gttccggcac accgcctcga
     2821 tccactcgac gaacgtcgac gccatcaccc gcatggggcg cgcgatgaac tgctcgatct
     2881 tcgtggcgaa cggcccgaac tacgccggcc tcggcgaggg gggcgagggc ttctgctcgt
     2941 tctccatcgc cagcccgacc ggcgacgggc tgacccggcc ccggaccttc tcccgcgagc
     3001 ggcggatcac cgtggtcggg tccctgcgga tcgtgtagcg gatgcagccg gccatcgcgc
     3061 tgctggagtt cgactcgatc gcccgcggga tcgcggccgg cgacgcgatg atcaagcggg
     3121 cccccctgga cgtgatccgc gccgggacgg tgcaccccgg gaagtacgtg gtcct
//
LOCUS       DASPWO010000435         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_484953, whole genome shotgun sequence.
ACCESSION   DASPWO010000435 DASPWO010000000
VERSION     DASPWO010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484953"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1818
                     /locus_tag="VEO00_09900"
     CDS             <1..1818
                     /locus_tag="VEO00_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYU58348.1"
                     /translation="EDVTVAELAPPACYLDGTTLNVGAGAVTLHRSGSSFDVTGAGIT
                     DPTCGGATMTNVDTVLVGGDAGAQSLTIDLGGGPLGPGATAEGSGTSEIEIIAALGPE
                     DSLTVNGGAGADKVAVGASGVNLNGDNDADVKPAVPATPAFSAITVNGNAGNDTISAT
                     GGSGTGPAYPSAITLNGGADADVLTGSAGPDSLAGGDGDDQLTGGTGANTVAGGTGAD
                     RLVEAGDVNFTLTNALLTFGPSTDALSGIELATLTGGAGANTLNGFGFGGQESLFGQA
                     GTDVLIGGGGADTLAGGDGDDRLSGAGGVNTLSGGAGSDVVAESGDVNFNLSNTNLTF
                     GSAVDSLAGVERAMLTGGAGANTLTAPGFSGAATLNGMGGADLLIGGNGADTLIGGDG
                     ADTLTGGRGANTVNGGAGNDQLSETGDVNFTLSNAALTFGSASDALSGVERAALTGGG
                     GDNTIDGSAFGGMLTLNGQGGSDRLIGGPAPDALSGGGGADVLAGRQGSNTISGGAGS
                     DTVVYDWSGAGVRVNLSTGSASGSAFSDLLSAIENAVGSAFADALTGAGGPNALRGLA
                     GNDTIDVRDGVRGNDMADGGAGTDRCLSDPGDARISCES"
     gene            1879..2226
                     /locus_tag="VEO00_09905"
     CDS             1879..2226
                     /locus_tag="VEO00_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HYU58349.1"
                     /translation="MQVQDVIAQARDTLTVKRVFGEPYERNGLTVIPVARVQGGGGGG
                     SGESPDGTGSGSGGGFGMSARPVGAFVIRDGEMTWRPAVDVTRIVLGAQVVAVVALLT
                     LRRIVKIRSKARS"
     gene            2341..2802
                     /locus_tag="VEO00_09910"
     CDS             2341..2802
                     /locus_tag="VEO00_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58350.1"
                     /translation="MALDEDLGFDALYADVGPRLWRAVLAYTGGARDLTDDVVAEAFA
                     RTLERGAAVRDRQAYLFRVAFRLAARELGRQPTVGEVPDAGTWDDPGLAEVFDALRGA
                     LARPARRAVPAVPGRPPGPRGGQADGDILRRGPDAPDARAAATGRRAGRRR"
     gene            2892..>4036
                     /locus_tag="VEO00_09915"
     CDS             2892..>4036
                     /locus_tag="VEO00_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58351.1"
                     /translation="MPAPDPGSGRRVAAAVLALAVAAGSFALVFSVFRGGGSRVGATT
                     PAGPTPAISTSPSTTDAGIPPPGAVLYPESICDVPEFDPDVALLVGQESVEYPRSVLE
                     APGGPASSLEGPASDELRAHLETPAARNAPADGWRVLWSSGSEVTFGAPFGEGEWWVD
                     AFEHREGGWRVFDEEIAKQRDTPAQRGHGMELRWDGPFAVEHGAWNDPLRLANEQALA
                     WTDPNDRFVAVPHVFDLSTGREIGPGADPIAAQGGPARLGRGEAAEIPVALGALVPSL
                     SPATYPIVACVPDLGLASPVGSLEVADDASVADVDVLTYHSNGVSMDALAGGELTVHH
                     GCLALAREVTDAGWTYLILPNGYALVRRGDREVLIRPTGEAVGAIGD"
BASE COUNT          537 a         1387 c         1583 g          529 t
ORIGIN      
        1 gaggacgtca cggtggccga gctggcgccg ccggcctgtt atctcgacgg gaccacgctg
       61 aacgtgggcg ccggagccgt cacgctgcac cgctcgggct cgagcttcga cgtgaccggc
      121 gccgggatca cggacccgac ctgtggcggc gcgacgatga cgaacgtcga caccgtgctg
      181 gtcgggggcg acgcgggggc gcagtcgctg acgatcgacc tcggcggcgg tccgctgggg
      241 cccggggcca cagcggaggg ctccggcacg tccgagatcg agatcatcgc cgcactcggc
      301 ccggaggact ccctcaccgt gaacgggggc gccggagcgg acaaggtggc ggtcggggcc
      361 tcgggggtga acctcaacgg cgacaacgac gccgacgtca agccggccgt tccggcgacg
      421 cccgcgttct ccgccatcac ggtgaacggg aacgcgggga acgacacgat ctccgcgacg
      481 ggcggctccg gcactgggcc cgcctacccc agcgccatca ccctgaacgg cggggcggac
      541 gccgacgtcc tcacggggag cgcgggtccc gactccctgg ccggcgggga cggcgacgac
      601 cagctcaccg gcgggacggg cgcgaatacg gtggccgggg gcacgggcgc cgaccgcctc
      661 gtcgaggccg gcgacgtgaa cttcacgctg acgaacgccc tgctcacgtt cgggccgtcc
      721 accgatgcgc tgtccgggat cgagctggcc acgctgaccg gcggcgccgg ggcgaatacg
      781 ctgaacgggt tcgggttcgg gggccaggaa tcgctcttcg ggcaggccgg caccgatgtc
      841 ctgatcggcg ggggcggtgc cgacacgctg gcgggcggtg acggcgacga ccggctctcc
      901 ggggcgggcg gcgtgaacac cctgagcggc ggcgcgggaa gcgacgtggt ggcggagtcg
      961 ggcgacgtca acttcaacct gtcgaacacg aacctgacgt tcggctcggc cgtggattcg
     1021 ctggccggcg tcgagcgggc catgctcacc ggcggcgccg gcgcgaacac gctgaccgcg
     1081 cccgggttca gcggggccgc cacgctgaac ggcatgggcg gcgccgacct cctcatcggc
     1141 ggcaacgggg ccgacaccct gatcggcggg gacggggcgg acacgctgac gggcgggcgg
     1201 ggggccaaca ccgtgaacgg cggggccggc aacgaccagc tgtccgagac gggggacgtc
     1261 aacttcaccc tcagcaacgc cgcgctgacc ttcgggtccg catccgatgc gctgtcgggc
     1321 gtcgagcgcg ccgcgctcac cgggggcggc ggggacaaca cgatcgacgg gtcggcgttc
     1381 ggcgggatgc tcacgctgaa cggccagggc ggcagcgacc gcctgatcgg cggccccgcg
     1441 cccgacgccc tgagcggtgg cggcggcgcc gacgtcctgg cggggcgcca gggatcgaac
     1501 acgatctcgg gcggggccgg ctccgacacc gtcgtgtacg actggtccgg ggctggtgtc
     1561 cgggtgaacc tctcgacggg ctcggcctcc ggatcggcgt tctccgacct cctgtcggcc
     1621 atcgagaacg ccgtggggtc ggccttcgcg gacgccctta ccggcgcggg cgggccgaac
     1681 gcgctccggg gcctggccgg aaacgacacg atcgacgttc gcgacggcgt ccgggggaac
     1741 gacatggccg acgggggagc gggcaccgac cgctgcctct ccgacccggg agacgccagg
     1801 atctcctgcg agtcctgatc cgccccgacc gcgcccgccg gcgtgccggc gcatgatgag
     1861 tgcggaagga ggcaggccat gcaggtacag gacgtcatcg cgcaggcgcg cgacacgctc
     1921 acggtgaagc gggtgttcgg cgagccatac gagcggaacg ggctgacggt gatcccggtg
     1981 gcgagggtgc agggcggcgg cgggggtgga agcggcgaga gcccggacgg aaccggatcc
     2041 ggctcggggg gcggcttcgg catgagcgcg cgaccggtcg gggcgttcgt catccgggac
     2101 ggcgagatga cctggcgtcc ggctgtcgac gtgacccgga tcgttctggg ggcccaggtc
     2161 gtggccgttg tggccctgct cacgctccgc cgcatcgtca agatcaggtc caaggcccgc
     2221 agctaggccg caggggccgc gtccgcgcgg ccgcccgctt tcacatgccc ggggttccgc
     2281 gaacccgtcg cccggcctgt tgacacgcgg ccgccgttcg ggctgtgtca gggagtgggg
     2341 ttggcgctgg acgaggacct ggggttcgac gcgctatacg ccgatgtcgg cccgcggctg
     2401 tggcgggccg tgctggccta cacgggcggc gcgcgcgacc tcaccgacga cgtcgtggcc
     2461 gaggcgttcg cccgcacgct cgaacgaggc gcggcggtca gggaccggca ggcctacctg
     2521 ttccgggtgg cgttccggct tgccgcccgg gagctcggac gacagccgac ggtgggcgag
     2581 gtccccgatg ccgggacgtg ggacgacccc gggctggccg aggtcttcga cgcgctccgg
     2641 ggcgctctcg cccggccagc gcgccgcgct gttcctgcgg taccaggccg acctcccggt
     2701 ccacgaggtg gccaggctga tggggacatc ctccggcgtg gtccggatgc acctgatgcg
     2761 agggcggcgg cgactggccg ccgagctggg agacgacgat gactgagcgc tggcgggagg
     2821 cactggatcg actggggcgg atcggcccgg accgtgatcg catcaggtct cgagccgagg
     2881 gtgggccacg gatgccggcg cccgatccgg gatcgggtcg ccgggtcgcc gcagcagtgc
     2941 tggccctagc ggtggcggcc gggtcgttcg ccctcgtgtt ctcagtgttc cgcggcggcg
     3001 gatcccgggt gggggcgacc acgccggcgg ggccgacacc tgccatctcc acgtcgccgt
     3061 ccacgaccga cgccggaatc cctccgcccg gcgcggttct gtaccccgag tcgatctgcg
     3121 atgtccccga gttcgacccg gacgtcgccc tgctggttgg ccaggagtcg gttgagtacc
     3181 cgaggagcgt tctggaggcg ccgggtggtc cggcctcgtc cctggagggt ccggcctcgg
     3241 acgagctgcg ggcccacctg gagactccgg ccgcgcggaa tgcgccggcc gacggatggc
     3301 gggtcctatg gtcgtcgggg tccgaggtca cgttcggcgc accgttcggc gagggggagt
     3361 ggtgggtcga cgcgttcgag caccgcgagg gcgggtggcg cgtcttcgac gaagagatcg
     3421 ccaagcagcg ggacacgccc gcccagcgag gccacggcat ggagctccga tgggacggcc
     3481 ccttcgcggt ggagcacggc gcgtggaacg acccacttcg gctggccaac gagcaggcct
     3541 tggcgtggac ggaccccaac gaccggttcg tggccgtccc gcacgtgttc gatctgtcga
     3601 ccggccggga gatcggcccc ggcgccgacc cgatcgcggc gcagggcggt cccgcgcgcc
     3661 tgggacgcgg cgaggccgca gagatcccgg ttgccctcgg cgcgctggtc ccgtcgctct
     3721 cccccgccac ctatccgatc gtggcgtgcg tcccggacct tgggctggcc tcaccggtcg
     3781 gatcgctcga ggtggccgac gacgcatccg ttgccgacgt cgacgtcctg acctaccact
     3841 cgaacggggt gagcatggac gcgctcgccg gcggcgagtt gaccgtccac cacggctgcc
     3901 tggcgctcgc ccgcgaggtg accgatgcgg gttggacgta tctgatcctg ccgaacgggt
     3961 acgcgctggt ccgacggggc gatcgggagg tgctgatccg ccccactggc gaggcggtcg
     4021 gagcgatcgg cgacga
//
LOCUS       DASPWO010000436         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_404345, whole genome shotgun sequence.
ACCESSION   DASPWO010000436 DASPWO010000000
VERSION     DASPWO010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404345"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VEO00_09920"
     CDS             <1..180
                     /locus_tag="VEO00_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58352.1"
                     /translation="GPNGWIHQINFVVLSPLTIAYAAGRTWACVPRAGVVGSAAPCPP
                     SVGCQGLGKVPGCAV"
     gene            235..>2044
                     /locus_tag="VEO00_09925"
     CDS             235..>2044
                     /locus_tag="VEO00_09925"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HYU58353.1"
                     /translation="MSSALRSLQVRPVERDELDEFKALLNAHHWLGYRMSGPPMRYVA
                     EINGVWVALATFGSAALKCPVREDYIGWGPGQRAGRLDHVVANQRFCVLPAGRVPNLA
                     SAVLGRILRRLDGDYRRVHDLAVLAVETFTDPSRHAGTCYAAAGFTPVGATGGYGRRR
                     GAEHYTFHGQPKTYWLRLLHPDALAVLAGGFPSPLLCDPPRRDLIELNVVEFGSLRRH
                     LARVSDGRKPRGVRHGQVTILLIAICALLSNNLNYTAIGEYAARLPQEALERLGARYH
                     KRRRRHIAPSESAIRRTIQRLDADELDAAIAAWVREQIGIGAMTNGDLRRFDKAAAPA
                     AAAGPPSDPPGAAGAAIALDGKTARGASNAKGGHKVHLFAALVHGTRAVIAQRGVDAK
                     TNEIPQVKPLLADLDLEGAVVTADALHTQTETARYIVEEKKADYVLEVKGNQPTLEGK
                     LIDLPREVFGPWTTVTERNRGRNEERRICVADTPADFAFPHCNQVFLLERYTRDDDGN
                     ILTAEAVTGITSLTSTKAGPDALLNHNRGHWGIEVLHNIRDVTFKEDASKIRTGAAPQ
                     VLAAIRNIGIFVLELAGHKNKAAGLRDLARHTLNLRA"
BASE COUNT          348 a          791 c          641 g          264 t
ORIGIN      
        1 ggacccaacg gatggatcca tcagatcaac ttcgtcgtgc tcagtccgct gaccatcgcc
       61 tacgcggccg ggcggacctg ggcctgcgtc ccacgcgccg gagtggtcgg ctcggccgcg
      121 ccctgcccac cttccgtcgg atgccaggga ctcggcaaag tccccggatg tgcggtctga
      181 cctgggcaga caggccggct ggacttactg gcgggtagct gatacgaaca ccgggtgtcc
      241 tcagctcttc gatcactgca ggtccgcccc gtcgaacggg atgagctcga cgagttcaag
      301 gctctcctga acgcgcatca ctggctcggc taccggatgt cgggtccgcc gatgcgctac
      361 gtcgccgaga tcaacggggt gtgggtggcg ctggcgacct tcgggtccgc ggcgctcaag
      421 tgcccggtcc gcgaggacta catcggctgg ggcccgggtc agcgcgccgg gcgactggac
      481 cacgtggtcg ccaatcagcg gttctgcgtg ctgccggcgg gccgggtacc gaacctggcc
      541 tcggccgtgc tggggcggat cctgcgccgc ctggacggcg actaccggcg cgtgcacgac
      601 cttgcggtgc tcgcggtgga aaccttcacc gacccgtccc ggcatgccgg gacctgctac
      661 gccgcggccg gattcacccc cgtcggcgcc accgggggct acgggcggcg ccgcggcgcc
      721 gagcactaca ccttccacgg gcagccgaag acctactggc tgcgcctgct gcacccggac
      781 gcgctggccg tgctggccgg cggtttcccc tccccgctgc tgtgcgaccc cccacggaga
      841 gacctgatcg agttgaacgt cgtcgagttc ggaagcctca gacgccacct cgcccgcgtg
      901 agcgacggcc ggaagccccg cggtgtccgc cacggccagg tcaccatcct gctgatcgcg
      961 atctgcgcgc tgctgtcgaa caacctgaac tacaccgcca tcggcgagta cgccgccagg
     1021 ctgccccagg aggccctcga gcggctcggc gcccgttacc acaagaggag gaggcgtcac
     1081 atcgcgccga gcgagtcggc gatccgccgc accatccagc gactcgacgc cgacgaactc
     1141 gacgcggcca tcgcggcctg ggtccgcgaa cagatcggga tcggcgccat gaccaacggc
     1201 gacctgcgac gcttcgacaa ggccgccgcc cccgccgcgg cggcgggccc gcccagcgac
     1261 ccccccggcg ccgccggcgc ggcgatcgcc ctggacggca agaccgcccg cggcgcctcc
     1321 aacgccaagg gcggccacaa ggtccacctg ttcgccgccc tcgtgcacgg cacccgcgcc
     1381 gtgatcgcac aacgcggggt cgacgccaag accaacgaga tcccgcaggt gaaacccctg
     1441 ctggccgacc tcgacctgga aggtgcggtc gtcaccgccg acgcgctcca cacccagacc
     1501 gagacagccc ggtacatcgt cgaggagaag aaagccgact acgtcctcga ggtgaaaggc
     1561 aaccagccca cgctcgaggg caaactcatc gacctcccac gggaggtctt cggcccgtgg
     1621 accaccgtca ccgaacggaa ccggggccgc aacgaggaac gccgcatctg cgtcgccgac
     1681 acccccgccg atttcgcctt cccgcactgc aaccaggtct tcctcctcga gcgctacacc
     1741 cgcgacgacg acgggaacat cctcaccgcc gaggcggtca ccggcatcac cagcctgacc
     1801 agcacgaagg ccggccccga cgcgctgctg aaccacaacc gcggccactg gggcatcgag
     1861 gtcctgcaca acatccgcga cgtgaccttc aaagaagacg catccaagat ccgcacaggc
     1921 gccgcaccac aggtactcgc cgccatccgc aacatcggga tcttcgtcct cgaactcgcc
     1981 ggccacaaga acaaggccgc cggactccgc gacctcgccc gccacaccct caacctccgc
     2041 gccc
//
LOCUS       DASPWO010000437         9022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_485002, whole genome shotgun sequence.
ACCESSION   DASPWO010000437 DASPWO010000000
VERSION     DASPWO010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9022
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485002"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VEO00_09930"
     CDS             <1..195
                     /locus_tag="VEO00_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58354.1"
                     /translation="PKDLAVGVPVEDVGSVTAAGAVNVLYGSPSGLTVTGNQLWTQDS
                     DGTDDAAESTDIFGGSLAST"
     gene            302..643
                     /locus_tag="VEO00_09935"
     CDS             302..643
                     /locus_tag="VEO00_09935"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYU58355.1"
                     /translation="MLLIYGDEQAQAGATPEQNQAVADEYEVFTKSIVGSGNFLDGDP
                     FHATSSAKTIQVRDGRTESVDGPAVTTDPQLLAYYKVEADSAEHAAELAARCPGSRYG
                     VIEVRPVVHFD"
     gene            730..3945
                     /locus_tag="VEO00_09940"
     CDS             730..3945
                     /locus_tag="VEO00_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU58356.1"
                     /translation="MLTCQACGESNPERARFCLACGSPLGAEASPAAEVRKTVTVVFS
                     DVTGSTAIGERLDPETLRRVMTRYFDEMRDAVQAHGGTVEKFIGDAVMAVFGIPLLHE
                     DDALRGVRAAAEMRDRLRVLNEELERDRGVTIAVRTGVNTGEVVAGDSTTGQPLVTGD
                     AVNVAARLEQAAAPGDILLGATTYALVRDAVEAEPLAEPLSLKGKAEPVAAHRLTHVR
                     PGVLGHARRLDSPMVGRERQRRLLDEAFDQAVSDRLCYLFTILGAAGVGKSRLVNEFL
                     SVARSGARIVSGRCLSYGNGITFWPLAEAFREAAGIADREPAADALAKLVSLVGDDPE
                     AGALAAHVAGLIGLAPADVPAEEAFRAVRRTFELMAAVRPLVVVFDDVHWAQPALLDL
                     IDHLAEWTRDASILLVCLARQELLELRPAWGGGKVAATTIRLEPLSDEESAALIGNLL
                     GSAGLDEGARRRIASAAEGNPLFVEEMLETLIDDGRLVRRNSHWEPAGDLSDLAVPPT
                     IQALLAARLERLGAEERSVMERGAVEGKVFHRGAVLELAPEPIRPALGAHLMALVRKE
                     LVRPDRPTFAGEEAFRFRHLLIRDAAYQAMPKETRADLHVRFAAWLERVAGERFPEYE
                     EIVAYHLEQAYRYREELGPVDEAARALAHRAAEHLAAAAGRANPRGDVLAARNLLERA
                     TALLPEGDALRLSLRPELGSVLWEAGEIEAAEELLTAAVAEAQAAGDRSSAAWARVYR
                     VELGASRGADIQASLAQARELIEEFEALGDEAGVAQAGAAAGQFLFFVGRAHEAETLL
                     SRTRDRALAAGVREPSVVATWWILTALFFGPAPVREAEERIARIEPELYRSRNNEAAL
                     LRTRARFAAIQGRFDEARRKLQAWADIEQELGRMSRLSSWEGHYIGPLEMAAGRFPEA
                     VVAFRTGFEALRALGDTGFSSTAAGGLAHALLEVGDVEEAERWARVALDTSAAGDIEP
                     KMAGTGALAVVLARQGKAEEAERTAREAVEMARRTDYVMAQAEVLVDLARVQAAAGRR
                     EEAQAAAAEAVELLERKEAWALVERAREAAASLVAPPAPSH"
     gene            3968..5023
                     /locus_tag="VEO00_09945"
     CDS             3968..5023
                     /locus_tag="VEO00_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58357.1"
                     /translation="MHHPPKGHLDTTFGGGDGMVVTGIPNLGVGAFGLAIVSGGKIVV
                     GGETFDPNGVRSDDLALIRYLPGGQLDTTFGGGDGKVTVDLFGGTDIFEEMTVDGNGR
                     AVIVTETEKNGDFVPALVRFEQNGSLDHSFSGDGAFVVPVPGTNSFMETVTTLGGGKI
                     VVGGEAVPQGGPDEDFLLMRVNAGGGVDTSFGTAGHVFTDFDNGADIADALRVDASGR
                     IVAGGQATVGGETELAVARYTGAGHRDTSFSGDGRVATDVGGFDRVFGLAIRSGKIVA
                     VGRSDTDGGNERWVVARYGSGGALDAGFGTDGRTITNFSTGPSEEEATGVVIESGGRI
                     VASGFTHNHPALAGYVG"
     gene            complement(5247..5558)
                     /locus_tag="VEO00_09950"
     CDS             complement(5247..5558)
                     /locus_tag="VEO00_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58358.1"
                     /translation="MRHTRIATYEITTGTFQEIADSAKSGLLPKLRELPGFVRYGVAD
                     IADNKLMSVSLWETHEQAEAASPVAAVWVKENIQDRVQLRENYVGDLAFWQGVKEPVG
                     A"
     gene            5821..6130
                     /locus_tag="VEO00_09955"
                     /pseudo
     CDS             5821..6130
                     /locus_tag="VEO00_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012181200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
     gene            6197..6436
                     /locus_tag="VEO00_09960"
     CDS             6197..6436
                     /locus_tag="VEO00_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HYU58359.1"
                     /translation="MARAVSVRLDDEALRALARLEATGLSRSQAIRKALLDAAERLER
                     RESLAAEVRALEADEADRMEMLEVAALMESLRAPG"
     gene            6423..6737
                     /locus_tag="VEO00_09965"
     CDS             6423..6737
                     /locus_tag="VEO00_09965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HYU58360.1"
                     /translation="MLRGDVYEFRPPRGRGHEQRGRRYGVVVQSDALLPRSVVIVAPT
                     SRSARPASFRPKVVVEDEVTRVLVEQIGAVDATRLGDLVSHVTPEELWGIDEALLTVL
                     GL"
     gene            complement(6960..7394)
                     /locus_tag="VEO00_09970"
     CDS             complement(6960..7394)
                     /locus_tag="VEO00_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HYU58361.1"
                     /translation="MSSTVDVNVLLYASDESSRFHEPARELVDRLAAGPELLYLFWPV
                     LMGYLRIATHPAIFTRPLPVEEAVANVEGLLSVPHVRALGEQDDFWRAYTSTTAGRVI
                     RGNLVPDAHLVALMRQNEVPSIWTHDRDFRTFEGIRVRDPFA"
     gene            complement(7391..7627)
                     /locus_tag="VEO00_09975"
     CDS             complement(7391..7627)
                     /locus_tag="VEO00_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HYU58362.1"
                     /translation="MARTTVNLDPSVLRGLKRRAREEGKSLGQLISEIVGPALAEGGR
                     TQERSPLRWHSAPMGPPKVDLEDKEAVRRALDDR"
     gene            complement(7691..8092)
                     /locus_tag="VEO00_09980"
     CDS             complement(7691..8092)
                     /locus_tag="VEO00_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58363.1"
                     /translation="MEVATTVISTAVLAVIGLIVTWVGKGQFNSIRREFRGKFDGIDA
                     RFDSIRQEFRARFDGVDARFDGSDHRFDGIDQRFDGIDHRFDAVDQRMNRLEERLDRR
                     MDAMQASIDGMRSDLTQLAVALGVQPRATNG"
     gene            complement(8256..8357)
                     /locus_tag="VEO00_09985"
                     /pseudo
     CDS             complement(8256..8357)
                     /locus_tag="VEO00_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            8538..>9022
                     /locus_tag="VEO00_09990"
     CDS             8538..>9022
                     /locus_tag="VEO00_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU58364.1"
                     /translation="MGAETTTQETWVGDLYEDLRPLMFAIAYRMLGSVTDAEDVVQEA
                     FLRFHRETKAGASIDSPKAYLSAVTTRLGIDHLRSARVRRETYVGPWLPEPLITDDEP
                     GAEQLAEIGDSLSMAFLVLLENLTPVERAVFLLREVFDYDYAEIAQVVGKTEDNCRQI
                     AV"
BASE COUNT         1327 a         3045 c         3357 g         1293 t
ORIGIN      
        1 ccgaaggatc tggcggtcgg cgtgccggtc gaggacgtcg gctcggtcac ggccgccggc
       61 gcggtcaacg tcctgtacgg ctccccatcc ggcctgacgg tgaccgggaa ccagctgtgg
      121 acccaggaca gcgacggcac cgacgacgcc gcggagtcca ccgacatctt cggcggatcg
      181 ctcgccagca cgtagggccg gcccgatcgg tagccggcct gtcgattcct cccgcccccg
      241 ttcgtcgtct gggcgcatcc ggggaactgc cccggagcgg aagggaggac ccgtgaagta
      301 catgctgctg atctacggcg acgagcaggc ccaggccggc gcgacgccgg agcagaacca
      361 ggccgtggcc gacgagtacg aggtgttcac gaagtccatc gtcgggagcg gcaacttcct
      421 cgacggcgac ccgttccacg ccacgagctc ggcgaagacg atccaggtcc gcgacggccg
      481 caccgagtcg gtcgacgggc ccgcggtgac gacggacccc cagctcctgg cctactacaa
      541 ggtggaggcg gacagcgccg agcacgccgc tgagctggcc gcgcgctgcc ctggctccag
      601 gtacggagtc atcgaggtcc gcccggtcgt gcacttcgac tgaccgttga caccgatcgc
      661 catatcgcct cctcgcggtc ccggcgcggg gaggcggtgg cacgctgggg gtgccctaca
      721 cttctggccg tgctgacctg ccaggcgtgc ggggaatcga acccggagcg cgcccgattc
      781 tgcctggcgt gcgggtcgcc gctcggggcc gaggcgtcgc cggcggcgga ggtacgcaag
      841 accgtcaccg tggtgttctc ggacgtgacg ggttcgacgg cgatcgggga gcgtctcgac
      901 cccgagaccc tccggcgggt gatgacgcgg tacttcgacg agatgcgcga cgcggtgcag
      961 gcccacggcg ggaccgtcga gaagttcatc ggcgacgccg tgatggcggt gttcggcatc
     1021 ccgctcctgc acgaggacga cgcgctccgc ggggtacgcg cggccgcgga gatgcgcgac
     1081 cggctccggg tcctgaacga ggagctggag cgcgaccgcg gcgtgaccat cgcggtgcgc
     1141 accggcgtca acaccggcga ggtggtggcc ggggattcga ccaccggaca gcccctggtg
     1201 accggcgacg cggtgaacgt cgccgcgcgc ctggagcagg cggccgcgcc gggtgacatc
     1261 ctcctgggcg cgacgacgta tgcgctcgtg cgcgacgcgg tggaggcgga gccgctcgcg
     1321 gagccgctct cgctgaaggg gaaggccgag ccggtcgcgg cccaccgcct gacgcacgtc
     1381 cggcccggcg tcctcggcca cgcgcgtcgg ctcgactccc cgatggtcgg ccgcgagcgc
     1441 cagcgccgcc tgctggacga ggccttcgac caggccgtct ccgaccgcct gtgctacctg
     1501 ttcacgatcc tcggcgccgc cggcgtgggg aagtcccgtc tggtgaacga gttcctgtcc
     1561 gtagcgagat ccggggcccg gatcgtctcc ggccgctgcc tgtcctacgg aaacggcatc
     1621 acgttctggc cgctggcaga ggccttcagg gaggcggccg gcatcgccga ccgggaaccg
     1681 gccgccgatg cgctcgcgaa gctcgtgtcg ctcgtcgggg acgaccccga ggccggggcg
     1741 ctcgccgccc acgtggccgg cctcatcggg ctcgcccccg ccgacgtccc cgcggaggag
     1801 gcgttccggg ccgtccgccg caccttcgag ctcatggcgg ccgtccggcc cctcgtcgtc
     1861 gtgttcgacg acgtccactg ggcgcagccg gcgctcctcg acctcatcga ccacctggcc
     1921 gaatggaccc gggacgcgtc catcctgctg gtctgcttgg cccggcagga gctgctggag
     1981 ctccgcccgg cctggggcgg aggcaaggtc gcggccacca cgatccggct ggaaccactg
     2041 tccgacgagg agagcgcagc gctcatcggc aacctcctgg ggagcgccgg cctggacgag
     2101 ggggcccgcc gccgcatcgc gtccgccgcc gagggcaacc cgctgttcgt cgaggagatg
     2161 ctcgagacgc tcatcgacga cggccggctg gtccggcgga acagccactg ggagccggcc
     2221 ggcgacctca gcgacctcgc cgtcccgccc accatccagg ccctgctggc ggcgcgcctg
     2281 gagcggctgg gtgccgagga gcgctcggtc atggaacgcg gggccgtcga gggcaaggtc
     2341 ttccaccgcg gcgcggtcct ggagctcgcg cccgagccca tccgccccgc gctcggtgcg
     2401 cacctcatgg cgctcgtgcg aaaggagctg gtcaggcccg accgccccac attcgccggc
     2461 gaggaggcct tccggttccg ccacctgctg atccgcgacg ccgcctacca ggccatgccg
     2521 aaggagaccc gggccgacct gcacgtgcgg ttcgcggcgt ggctggagcg ggtggccggc
     2581 gagcgcttcc ccgagtacga ggagatcgtc gcctaccacc tggagcaggc ctaccgctac
     2641 cgcgaggagc tcggcccggt cgacgaggcc gcccgcgcgc tggcccacag ggccgccgag
     2701 cacctcgccg cggcggcggg ccgcgcgaat ccgcgtggcg acgtgctcgc cgcgcgcaac
     2761 ctcctcgagc gggcgacggc gctgctcccc gaaggggatg cgcttcggct gtccctgcgg
     2821 ccggagctgg gaagcgtcct gtgggaggcg ggcgagatcg aggcggcgga ggagctgctg
     2881 acggcggcgg tggccgaggc gcaggcggcg ggagatcgtt cgtccgcggc gtgggcgcgg
     2941 gtgtaccggg tcgagctggg ggcctcgcga ggggcggaca tccaggcgtc gctggctcag
     3001 gcccgggagc tgatcgagga attcgaggca ctgggcgacg aggccggggt ggcacaggcc
     3061 ggggccgcgg cgggccagtt cctgttcttc gtgggccgcg cgcatgaggc ggagacgctg
     3121 ctctcccgga cgcgcgaccg ggcgctcgcc gccggagtgc gggagccctc ggtggtggcg
     3181 acgtggtgga tcctcacggc gttgttcttc gggccggcgc cggttcgcga ggccgaggag
     3241 cgcatcgctc ggatcgaacc cgagctgtac cgctcccgca acaacgaagc ggcgctgctc
     3301 cggacgcgag ccaggttcgc ggcgatccag ggccgcttcg acgaggcccg ccggaagctg
     3361 caggcctggg ccgacatcga acaggagctc ggccgcatga gccgcctgtc gtcgtgggag
     3421 ggccactaca tcggcccact cgagatggcc gccgggcgat tcccggaagc cgtcgtggcg
     3481 ttccgaacgg ggttcgaggc gctgcgggcg ctcggggaca ccggattctc ctcgaccgcc
     3541 gccggcgggc tggcccacgc cctgctcgag gtcggggacg tggaggaggc cgagcgctgg
     3601 gcgcgggtcg ccctggacac ctcggccgcg ggcgacatcg agccgaagat ggcgggcacc
     3661 ggcgccctgg ccgtcgtcct ggcccgccag ggcaaggcgg aggaggccga gcgcaccgcg
     3721 agggaggccg tcgagatggc gcgccggacc gactacgtga tggcccaggc ggaggtcctc
     3781 gtggacctgg ccagggtgca ggccgcggcg ggccgtcgcg aggaggcgca ggccgcagcg
     3841 gccgaggccg tcgagctgct ggaacggaag gaggcctggg ccctggtcga acgcgcgcgc
     3901 gaggccgccg cctcgctcgt tgctccgcct gccccgtcgc actagggtcc tcctcccgcg
     3961 cgttgatttg caccacccgc cgaagggcca cctcgacacc acgttcggcg gcggggacgg
     4021 catggtggtc acgggcatcc cgaacctcgg cgtgggtgcc ttcgggctcg cgatcgtctc
     4081 gggcgggaag atcgtggtgg ggggcgagac gttcgacccg aacggcgtga gatcggacga
     4141 cctggccctc attcgctacc tccccggcgg acagctggac accacgttcg gcggcgggga
     4201 cgggaaggtc accgtggatc tgttcggcgg gaccgacatc ttcgaggaga tgacggtcga
     4261 cgggaacggc agggcggtga tcgtcaccga gaccgagaag aacggcgact tcgtccccgc
     4321 cctggtccgg ttcgagcaga acggcagcct ggaccattcg ttcagcggcg acggcgcgtt
     4381 cgtcgtgccc gtgccgggca ccaattcctt catggagacc gtcaccacgc tggggggcgg
     4441 aaagatcgtc gtgggcgggg aggccgtccc gcaggggggc cccgacgagg acttcctgct
     4501 gatgcgggtg aacgcgggcg ggggcgtcga cacatcgttc ggaacggcgg gtcacgtctt
     4561 caccgacttc gacaacggag cggacatcgc cgacgcgctc cgggtggacg ccagcggcag
     4621 gatcgtggcc ggcggacagg cgacggtcgg cggggaaacg gagctggccg tggctcgcta
     4681 caccggcgca gggcatcggg acacgtcgtt cagcggcgac gggcgcgtgg ccacggacgt
     4741 cggcggcttc gaccgcgtgt tcggcctcgc gatccggagc gggaagatcg tggccgtggg
     4801 ccggagcgac accgacgggg gcaacgagcg gtgggtcgtg gcgcggtacg gttcgggcgg
     4861 cgccctcgac gccgggttcg gcaccgacgg gaggaccatc acgaacttct cgacgggccc
     4921 gtcggaggag gaagccacgg gggtggtcat cgagtccggc ggtcggatcg tcgcctcggg
     4981 gttcacgcac aaccaccccg ccctcgccgg gtacgtggga tagggccgtc tcaggcacgg
     5041 ttgtgggtgg cggcgtcgag tggtgcccca tcgccgacaa cccccggcgg gcgggcaagc
     5101 cgctccgcgg agagcgaccg ccgacgccga gcgatgtctc aagtcgtgcg cgtaacgagc
     5161 gagggggccc acctcacggt ggacccccgt cgtggtgctg aaccgatcgg gctcgggctc
     5221 ggcgctcggc cgaggtcccg aatcggtcag gcacccaccg gttccttgac tccctgccag
     5281 aaggcgaggt ccccgacgta gttctcgcgc agctggacgc ggtcctggat attctccttg
     5341 acccacacag cggcgacggg cgaggcggcc tcggcctgct cgtgcgtctc ccacaggctg
     5401 accgacatga gcttgttgtc ggcgatgtcg gcgacgccgt accggacgaa gcccggcagc
     5461 tcgcggagct tcggcagcag ccccgacttc gcggagtcgg cgatctcctg gaacgtaccg
     5521 gtcgtgatct cgtacgttgc gattcgcgtg tggcgcatgt cgcgctcctt ctagatttct
     5581 agtgctcgtc gttcggcgtc caggcgtgca cggccgagca tggaggtcat ctcgaacggg
     5641 tcgcactgag cgccgcatcg gagcgggttc ggaaggaggc gatcccgtag gtgagttcta
     5701 ctcccgtcag gaacggggtc gcttctccat cgagtgtacg tcttccgccg aggcccgggc
     5761 cgccgtccgc acccgagggt gcggcgcgca ccccgatcgg agctatcgtc gcccgagccg
     5821 atgaggagcc agccggaaga agaggtgttc gacagcccga gcggctgggt ggccgcgcac
     5881 gtccgccgct acatcgagac cgacggcaag gacggccacc ggtggcgagg ggtgacctga
     5941 ccggcaatcc cgaggtcgag atccaggtcg gcgccgaccg gttcccggcg agagcccgca
     6001 ccgcgaccgc ggaggagaag cccgaactct ggcggatcat gacgtccatc tggcccgact
     6061 acgaccgata tcaggccagg accgagcggg acatccggtc gtgatcctgg agcgccgctc
     6121 ctccgggtag cgcgtctcca tcgaagaaca cccactcggc ctggctcgta tgacgaaacg
     6181 tgatacgctc tcgagcatgg cgagagcggt gtccgttcga ctggacgacg aggcccttcg
     6241 ggccctcgct cgcctggagg cgaccggact cagccggtcg caggccatcc ggaaggccct
     6301 cctcgatgcc gccgagcgtc tggagcggcg ggagtctctc gccgccgagg tgcgggcgct
     6361 cgaggccgac gaggccgacc ggatggagat gctcgaggtg gccgccctga tggagagcct
     6421 gcgtgctccg gggtgacgtg tacgagttcc gcccgccgag ggggcgcggt cacgagcagc
     6481 gcggccgccg atacggcgtc gtggtccagt cggacgcgct gctcccgcgc tccgtggtca
     6541 tcgtcgcgcc gacctcccga tcggcccgac ccgcctcgtt ccgaccgaag gtcgtcgtgg
     6601 aggacgaggt gacgcgggtc ctcgtcgaac agatcggcgc cgtcgacgcc acccggctcg
     6661 gtgacctcgt cagccacgtg accccggagg agctgtgggg gatcgacgag gccctcttga
     6721 cggtcctcgg tctgtagtcg tcggaggcgg tcggtgcagg acgaacgctg gcggcgcgag
     6781 gcagaacccg aggagagacc tcgtgatatc agccggtcgc cgtagttctc gaggatcttc
     6841 tccgggccga acaggcggca gaaggcgccg gcgtcggacg cgtatgcctt gtcgtaccgc
     6901 tgacgctcta accggcaggt cgcccggtag gggccgggcc atgagcccgc gcgcggctct
     6961 caggcgaacg ggtctcggac ccggatgcct tcgaacgtgc ggaagtcgcg atcgtgggtc
     7021 cagatcgacg ggacctcgtt ctggcgcatg agggcgacga ggtgggcgtc cgggaccaga
     7081 ttgccccgga tgacccgtcc agcggtcgtc gacgtgtatg cgcgccagaa atcgtcctgt
     7141 tcgcccagtg cccggacgtg gggcacggac aggagcccct cgacgttggc gaccgcctcc
     7201 tcgacgggaa gcgggcgagt gaagatcgcc gggtgggtgg cgatccgcag gtagcccatg
     7261 aggacgggcc agaagagata gaggagctcg ggcccggcgg cgaggcggtc gacgagctcc
     7321 cgcgccggtt cgtggaatcg actcgactcg tcggaagcgt agagaaggac gttgacgtcg
     7381 accgtcgagc tcaccggtcg tcgagcgccc gccgaaccgc ctccttgtcc tcgaggtcga
     7441 ccttcggcgg ccccatcggg gcggagtgcc agcgcagagg agaccgctcc tgtgttcgac
     7501 caccctccgc cagcgcgggc ccgacgatct cggagatgag ctgacccagg ctcttcccct
     7561 cctcacgagc ccggcgcttg agcccgcgaa ggacggaggg atcgaggttg acggtggtcc
     7621 gcgccatgat gcttgatgct agcgatcttc gcatcagatg tccattagag cggctatcgg
     7681 gcgtggtggg ctatccgttc gtggccctgg gctgaacgcc aagagcaacg gcaagctggg
     7741 tgaggtcgga gcgcatcccg tcgatcgacg cttgcatggc gtccatacgt cggtccagac
     7801 gctcctccag ccggttcatg cgttgatcca ccgcgtcgaa ccgatgatcg atcccatcga
     7861 accgttgatc gatcccgtcg aaccgatgat cgctcccgtc gaaccgggca tcgaccccgt
     7921 cgaaccgggc ccggaactcc tgccggatcg aatcgaaccg ggcatcgatc ccgtcgaact
     7981 tgccccggaa ctcccgtcgg atcgaattga actgaccctt tccgacccag gtgacgatca
     8041 gcccgatcac cgccaggacc gcggtgctga tgacggtggt ggccacttcc atggcgatgg
     8101 tccctctccg ggggaggaac ggtgtgcgtt cgttcgaagg gtagcagggg gcacctacaa
     8161 gctccgcggt ctgtctggtc aggtctcctg cctctccttc ccgtcgccgc cggttgcggc
     8221 gccctcgagg gttcggccgg tcttggccag gaacgcccgg tggtgggctc gtcgaggaac
     8281 agcacggcgg gggagtggat cagcgcgagc gccaggtcaa ggcgccgctt catcccgccc
     8341 gagtacgtgg cgacctttgc ggtcggcggc gtcgatgagc ccgaccgagc gcccgggcac
     8401 cgccgttcga ttcgaacatg aggacctcgc ctccttcggg tgatgtcgtc gaacccggag
     8461 acgaggcagg ggtgcgccct gtgacagggg gagggcgatg tcacagggag gcgggctgtc
     8521 tcgtcttgag attcagaatg ggagcggaga cgacgaccca ggagacgtgg gtgggagacc
     8581 tgtacgagga cctccggccg ctgatgttcg cgatcgcgta ccggatgctc ggcagcgtga
     8641 ccgacgcgga ggacgtggtg caggaggcgt tcctccggtt ccaccgggag acgaaggcgg
     8701 gcgcgagcat cgactcgccg aaggcctacc tgtcggcggt gacgacgagg ctcgggatcg
     8761 accacctgcg gtcggcgagg gtgcgccggg agacctacgt cggcccgtgg ctccccgagc
     8821 ccctgatcac cgacgacgag cccggggcgg agcagctggc cgagatcggc gacagcctgt
     8881 cgatggcgtt cctggtgctg ctggagaacc tcacgccggt cgagcgagcg gtgttcctgc
     8941 tgagagaggt cttcgactac gactacgcgg agatcgccca ggtggtcggc aagaccgaag
     9001 acaactgccg ccagatcgcg gt
//
LOCUS       DASPWO010000438         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_912852, whole genome shotgun sequence.
ACCESSION   DASPWO010000438 DASPWO010000000
VERSION     DASPWO010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912852"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="VEO00_09995"
     CDS             <1..1571
                     /locus_tag="VEO00_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HYU58365.1"
                     /translation="FILTLGGVGFVRAAKRIYQVVTLTGRRRGEARRVLVVGGGRAGE
                     KLIREMVHSRVGGMRPVGIIDDDKFKIGTYLHGIRVIGPRSLIPDVVRDERVDDVMIA
                     MPSAPGHVIREVVVAARSAGAPSVQIIPGVAAMLEGKIALASVREVKVEDLLNRPPVE
                     LDMATVGETLYGKTVLVTGAAGSIGSELARLIATYDPGRLVLLEKDETRLFGLELDLR
                     RLAPRVPVFPVVADVRSPGRMSQVCRQYEPQVVFHAAAYKHVPMMEAHPEEAVTTNVL
                     GTRALAEAAAEAGAETFVLISTDKAVNPTSVMGATKRVAEMVIRRANETSATRFMAMR
                     FGNVLGSRGSLVPILEEQIQRGGPITVTHPDMERYFMTATEAARLILKASTIGRGGEV
                     FVLDMGNPVRVLDLVEALIRLSGLEPDVGVPIQFTGTRPGEKLHEEILLAEEGTEATS
                     AEQIFTGILGEEPDPAGLEHGLAMLGDAAELGDGKRIRFLLQEVVRTYTPAPGGHGDE
                     PLAELLEGPLDDGA"
     gene            1564..2511
                     /locus_tag="VEO00_10000"
     CDS             1564..2511
                     /locus_tag="VEO00_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYU58366.1"
                     /translation="MPDRHMVTGGAGFIGSNLVHALLDEGFAVRVLDDFSTGRKENLA
                     GVAGDVEVVEGGVADPAAVARAMDGVAVVYHQAAIPSVSRSVSDPVATHEANATGTLN
                     VLIAARDAGVSRVVYASSSSVYGDTQALPVREDLPAAPLSPYGVAKLAGERYCTAFHA
                     SYGLPTVALRYFNVFGPRQDPSSEYAAVVPRFATAVLAGVPPTVFGDGEQSRDFTYVG
                     DVVQANRKAETAPDGAFGAVFNVGQGGRTTINRLLEMLQDLAGVERGDPVKAPARKGE
                     TRHSQADITRARQVLGFDPAVSLEEGLRRTLDWFRDHPG"
BASE COUNT          421 a         1021 c         1100 g          430 t
ORIGIN      
        1 agttcatcct cacgctcggc ggcgtcggct tcgtccgggc cgcgaagcgc atctaccagg
       61 tagtcacgct gaccgggcgc cgccggggcg aggcgcggcg cgtgctcgtg gttggcggcg
      121 gccgcgccgg ggagaagctg atccgggaga tggtccactc ccgggtcggc gggatgcgcc
      181 cggtcgggat catcgacgac gacaagttca agatcgggac ctatctgcac ggcatccggg
      241 tgatcggccc gcgctcgctg atccccgacg tcgtgcgcga cgagcgcgtc gacgacgtga
      301 tgatcgccat gccgtcggct ccgggacacg tcatccgcga ggtggtcgtg gcggcgcggt
      361 cggccggcgc gccgtcggtg cagatcatcc cgggcgtggc cgcgatgctc gagggcaaga
      421 tcgcgctggc gtcggtgcgc gaggtgaagg tggaggacct gctgaaccgg ccgccggtcg
      481 agctcgacat ggcgacggtc ggggagacgc tgtacggcaa gaccgtcctg gtcaccggcg
      541 cggccggctc gatcggctcg gagctcgccc ggctgatcgc cacgtacgac ccgggccggc
      601 tggtgctgct ggagaaggac gagacccggc tgttcggcct ggagctcgac ctgcgccggc
      661 tggccccgcg cgtcccggtg ttcccggtgg tggcggacgt gcggagcccg ggacggatgt
      721 cccaggtctg ccgccagtac gagccgcagg tggtgttcca cgcggcggcc tacaagcacg
      781 tcccgatgat ggaggcccac cccgaggagg cggtgaccac gaacgtcctc ggcacgcgcg
      841 ccctggccga ggcggcggcg gaggccgggg ccgagacgtt cgtgctgatc tcgaccgaca
      901 aggcggtcaa cccgacgtcg gtgatgggcg cgacgaagcg ggtggccgag atggtgatcc
      961 gccgggccaa cgagacgagc gccacgcggt tcatggcgat gcgcttcggc aacgtcctcg
     1021 gcagccgcgg cagcctggtg ccgatcctcg aggagcagat ccagcgcggc gggccgatca
     1081 ccgtgaccca cccggacatg gagcgctact tcatgaccgc caccgaggcg gcccggctga
     1141 tcctgaaggc atccaccatc ggccgcggcg gcgaggtgtt cgtgctcgac atggggaacc
     1201 cggtgcgcgt gctcgacctg gtcgaggcgc tgatccgcct ctcggggctg gagccggacg
     1261 tcggcgtccc gatccagttc accggtaccc gcccggggga gaagctgcac gaagagatcc
     1321 tgctggccga ggagggcacc gaggcgacga gcgccgagca gatcttcacc ggcatcctcg
     1381 gcgaggagcc ggaccccgcc gggctggagc acggcctggc gatgctgggc gatgccgccg
     1441 agttggggga cggcaagcgg atccgcttcc tgctgcagga ggtcgtgcgc acctacacgc
     1501 cggcccccgg cggccacggc gacgagcccc tggccgagct cctcgagggg ccgctggacg
     1561 acggtgcctg atcggcacat ggtcaccggc ggcgccgggt tcatcggctc gaacctggtg
     1621 cacgcgctcc tcgacgaggg gttcgcggtg cgcgtgctcg acgacttctc caccgggcgc
     1681 aaggagaacc tggccggggt cgccggcgac gtcgaggtgg tcgagggggg cgtcgccgac
     1741 ccggcggccg tcgcgcgggc gatggacggc gtggccgtcg tctaccacca ggccgccatc
     1801 ccgtcggtgt cgcgctcggt cagcgacccg gtcgcgacgc acgaggcgaa cgccaccggg
     1861 acgctgaacg tgctgatcgc cgcgcgcgac gccggcgtgt cgcgcgtggt ctacgcgtca
     1921 tcctcgtcgg tgtatggcga cacccaggcg ctgccggtgc gcgaggacct cccggccgcg
     1981 ccgctctccc cctacggtgt ggcgaagctc gccggagagc gctactgcac ggccttccat
     2041 gcctcctacg gcctgccgac cgtggccctg cgctacttca acgtgttcgg cccgcgacag
     2101 gacccgtcct cggagtacgc ggccgtcgtc ccgcgattcg ccacggcggt cctggccgga
     2161 gttccgccga ccgtgttcgg cgacggcgag cagagccgcg atttcactta cgtcggcgac
     2221 gtcgtgcaag ccaaccggaa ggccgagacg gcccccgacg gggcgttcgg cgccgtcttc
     2281 aacgtcggcc agggcgggcg gacgacgatc aaccggctgc tcgagatgct gcaggacctg
     2341 gccggggtgg agcgcggcga cccggtgaag gccccggccc gcaaggggga gacccggcac
     2401 tcgcaggccg acatcacccg cgctcgccag gtgctcggct tcgacccggc cgtgtcgctc
     2461 gaggagggcc tgcgccgcac cctcgactgg ttccgcgacc acccgggctg acgcggccgg
     2521 acgagcccca atcgagaatg gaactcatga ctcctcttga tcctgactgc accggtgagt
     2581 ccggattctg caacatcccc gatccgcagg cgatgtcgga ttccaagacg ttgagcaccg
     2641 cacatataga cgaggacttc gatccgggcc tgccttccgg caacatggtc gagatcgggt
     2701 ggatcgttga gtggaacggt tctcgcaggt ggacggccga gtgctcttgc gggcgatcgt
     2761 cggcatcggc ggctccgtcg tggcgctctt cctctacatc ctggggtcga gcgaggagtg
     2821 aatcggatcg gagccacccc gagtcggtag tgggcgcctg gtgccggcgg cggggcggcc
     2881 ggtcctgctc ctatgatccc cggaccatgg cgcggctcac gccctcggcc ggctacacgc
     2941 tcacgctgcg cgtcaacctg gtgaacgtgc cg
//
LOCUS       DASPWO010000439         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_404886, whole genome shotgun sequence.
ACCESSION   DASPWO010000439 DASPWO010000000
VERSION     DASPWO010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404886"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1245)
                     /locus_tag="VEO00_10005"
     CDS             complement(241..1245)
                     /locus_tag="VEO00_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU58367.1"
                     /translation="MELGVVVGGDLTGIGDLARRAEDAGYESLWVAETARSAFVQAAV
                     AGAATERVTVGTAIALAFPRSPAIAAMAARDLSELTAGRFVLGLGSQVKRVNEHRFGV
                     PFEHPAPKMREYLLAVRAVLGSFGGAVQPFEGEFYRITMAPFPGAGPAPGPVPIYLAA
                     VNERMCEVAGEVADGVHGHPLNSPRYLAEVVRPAVARGSARAGREPADVSITTNVIVQ
                     LSADREAARREAAMQLAFYATTRTYAPVLAFHGHEERIGPLRRAFVEEDWERMAELAL
                     PMVDDLAIAGDADECRERLAAYSGAADRVVLGGAWVGPSPDRVADNYRRILETLGPAS
                     "
     gene            complement(1258..1695)
                     /locus_tag="VEO00_10010"
     CDS             complement(1258..1695)
                     /locus_tag="VEO00_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYU58368.1"
                     /translation="MAPTSARGSARIIEALRSVPLFAHCTKRELQEIASASKIIERPA
                     GTVLAREGERGVGFFLIIDGHAIVTVGGRKRADVGPGQFFGEISLIDEGPRTATVTAT
                     TPVATLGMTAWTFRGLIHDHPGIALRMLEVVAGRLRASAGANA"
     gene            complement(1729..2338)
                     /locus_tag="VEO00_10015"
                     /pseudo
     CDS             complement(1729..2338)
                     /locus_tag="VEO00_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
BASE COUNT          327 a          879 c          835 g          297 t
ORIGIN      
        1 taggcagccc ggacgacccg ccgaaaaccg gtgccctgac gtgaagggat tcgccgggga
       61 ccgtcgaact ctacgagtga gcgacggccc cgtatgcccg cgggggccgt ctccgggccg
      121 aaagcccatc gcccggaggc ccgccggccg ttcgcgcggc ccgcgaccgc ccgcgcgggg
      181 ctccccggga ggcacgcgcc ccgtggcctc ccggggatgg cccccgcccg gggcggtcgt
      241 ctacgaggcc gggccgagcg tctccaggat ccggcggtag ttgtccgcca cccggtcggg
      301 gctggggccg acccaggccc cgccgagcac cacccgatcg gcggcccccg agtaggccgc
      361 cagccgctcg cggcactcgt cggcgtcccc ggcgatcgcc aggtcgtcca ccatgggcaa
      421 cgccagctcg gccatccgct cccagtcctc ttcgacgaag gctctgcgca gcggcccgat
      481 ccgctcctcg tggccgtgga aggcgaggac cggggcgtag gtgcgggtgg tggcgtagaa
      541 ggccagctgc atggccgcct cgcgccgggc cgcctcgcgg tccgcggaga gttgcacgat
      601 cacgttcgtg gtgatcgaga cgtcggccgg ttcccggccg gcgcgcgccg atccccgggc
      661 cacggcgggg cggaccacct cggccaggta gcgcggcgag ttcagcggat ggccgtggac
      721 gccgtcggcg acctcccccg ccacctcgca catccgctcg ttcaccgcgg ccaggtagat
      781 cgggaccgga ccgggggccg ggccggcgcc ggggaagggc gccatcgtga tccggtagaa
      841 ctcgccctcg aacggctgca ccgcgccgcc gaacgacccg agcacggctc gtacggccag
      901 caggtactcc cgcatcttcg gggccgggtg ctcgaacggg acgccgaagc gatgctcgtt
      961 cacccgcttc acctgcgagc cgagcccgag aacgaagcgg ccggcggtga gctcggacag
     1021 gtcgcgggcg gccatcgccg cgatcgccgg gctgcgcggg aaggccaggg cgatggccgt
     1081 ccccaccgtg acccgctcgg tggcggcacc ggccaccgcg gcctgcacga acgccgaccg
     1141 cgcggtctcc gcgacccaga ggctctcgta cccggcgtcc tcggcgcgcc gggccagatc
     1201 gccgatcccc gtcaggtcgc caccgacgac gaccccgagt tccaccagga acggtcgcta
     1261 ggcgttggcg ccggcggagg cgcgcaggcg accggccacg acctcgagca tgcgcagggc
     1321 gatcccaggg tggtcgtgga tcaggccgcg gaacgtccag gcggtcatcc ccagcgtggc
     1381 gaccggcgtg gtcgcggtca ccgtggcggt gcgcgggccc tcgtcgatca gcgagatctc
     1441 cccgaagaac tgaccgggcc cgacgtccgc gcgcttgcgc ccgccgacgg tcacgatggc
     1501 gtgcccgtcg atgatcagga agaacccaac gccccgctcg ccctcccgcg ccaggacggt
     1561 tcccgcggga cgttcgatga tcttcgacgc gctggcgatc tcctgcagct cgcgcttggt
     1621 gcagtgcgcg aacagcggga cgctccgcag ggcctcgatg atccgggcgc tgccccttgc
     1681 gctggttggc gccatctgca ctgccccctc aggttggatc gggtgatttt acgaacgggc
     1741 gggctccgcg tcgagggagc cggcctgtga gatcgcccac gccgcgttga tcagcccgat
     1801 gtggctgaag gcctgcggga agttcccgag gagctcgccg gaatcctcat cgacctcctc
     1861 ggagagcaag ccgacgtcgt tggcgaacgc gatcgcccgc tcgaacaccg tccgtgcccg
     1921 ctcggggcga ccggccaggg ccagcgcctc ggccagccag aacgtgcaga ggaggaacgt
     1981 cccctcctcg ccgggcaggc cgtcgtccgc caggtagcga tacaccaggc ccgtggtcgt
     2041 cggtcaggcg tccctcgatc gcttcgatcg tggcgatcat ccgcgggtcg tcggccggga
     2101 ggaagccgac gatcgggagc atcagggtcg atgcgtcgag gtcatcggag ccgaacgact
     2161 gcgtgaaggc acccgcccgc tcgctccagc cccgctccag gatcgccgca cggatctcct
     2221 cgcgcacgcc gccccactcg gcgacgcggt cctcggcgcc gaggagcccc gccagccgga
     2281 tcccccggtc gagcgcgacc cagcacatca gcttggagta gacgaagtgg cgcggctc
//
LOCUS       DASPWO010000440         5086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_485739, whole genome shotgun sequence.
ACCESSION   DASPWO010000440 DASPWO010000000
VERSION     DASPWO010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485739"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VEO00_10020"
     CDS             <1..384
                     /locus_tag="VEO00_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58369.1"
                     /translation="SAAETVREIEETRDRLDGELRALEDRLPRPAVWTKRIAGVAIGG
                     GVAGAAFWFLVRRRRAKRVPSTSANVQAVVHVLPEGLAARLEHAVEDGRWRGTAMGVA
                     GVWLALRLAELRQLRRMNRALLAAR"
     gene            452..2197
                     /locus_tag="VEO00_10025"
     CDS             452..2197
                     /locus_tag="VEO00_10025"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HYU58370.1"
                     /translation="MSGSRYVLVGLSRIAVRVADVLAARGDEVVVVPEPSGGDTAAET
                     LLRHRVRVMEPTPDREEALRAAGVDGADCLLAVSDDDLENLHAALAAGAVAPDVPLVI
                     RSFEPVLVDRLARSVRLRRAYSVSALAAPAFVAAALGEDLLATLRLGEDDISLCRLEV
                     RPGAPIAGATAAEVKADSGCAVVAHDDGSGWRPCAPVERITEGERILIGGPLRLALLL
                     AKRVSPVLGKDRARPAPGLLHRLRDRRAERKALHAGLRANTLLPAAAAVLVGLLLITV
                     IVFAVALHLNPIDAIYFAVSTALGNATLDQNEAWLKAFGIGAMVAGGALLGVVFSYLA
                     ALATAERLEEQMGRRARRLSNHVVLAGLGTVGYRIEHLLSDLEIPLAVIERSPDDRFV
                     ATVARRTPVVIGDARLPEDLMRVGVEDAACFVACTEDELTNVAACLQAQRQNPGLRTV
                     ARVFDEELGGRIQTTLGIDAAVSGTRAATGAFVSAAVDERAPRTLHLGGVAFVAVRFE
                     PTVPLDAGEIDRWRGRGLRVLAFSRGNGPVRAASELDGGLGPGDAAIVAGPEAAVRET
                     LFGAARPGIADRLRP"
     gene            2194..3123
                     /locus_tag="VEO00_10030"
     CDS             2194..3123
                     /locus_tag="VEO00_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58371.1"
                     /translation="MTADQEAPAGEPVTRPRRFSTARRAKVALQAAAAVLALVAVKLI
                     VHHFDLEFIELSPLYTSIVAGGIFVIGLLVAGTLADYKESERMPAEIAASLESIYEDC
                     RSIHEGTPAFDLARLRSLLGRVVSSLRYDLADPRFTRHCLDALREVSASFLELERLGV
                     PANYVVRLRTEQGSIRRNVLRVYHIQRTDFLPSGYLLIQTMVVLILAALVFTKIEPLS
                     LAIVVLVFLTYFFVYLERLLRLLDRPFRLDERTPDDVSLFLLDAFAHHLRGGFGEVAP
                     PLERETPAPPASGPPKEESPREAGGREERFGVP"
     gene            3192..4868
                     /locus_tag="VEO00_10035"
     CDS             3192..4868
                     /locus_tag="VEO00_10035"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HYU58372.1"
                     /translation="MRRKSLLVTFSPRSRIWFGIVLPVLAVAGVTAALWASGPYPAVI
                     PGLLLLIPVTAAGLLGGFVAGIVVTVSASLGFLLLLPPIGRLALGGRLPNVEGPGYST
                     DVVVMLAFVGVSLALVRETSWERHQRRRAETEREQLAFLATASEWLHGTLDARDALER
                     VANLAVPRLADGCTIEIVRDDGTLDTVAMAFSDPEKAALARRMRERFPPRPGDPGGVW
                     EAIREERATVVPRVARRILRQFAPSEEQFDLASRLRPRSIIVAPLVAQGPPIGAVTLV
                     MAESGRRYLRGDLTFVQDLALRAATAVENARRYTERSRIASTLQRALLPEALPEIPFA
                     RVAHRYQAAADRVGGDFYDAIRSGDRSWSVFIGDVRGRGVEAASLTALARHTIRTAAL
                     LERGPAAALSILNEAILRRGDPESFCTLAYVCLYPTASGVVARIACGGHPPPLVLRGQ
                     GAVEVAGATGTLLGVFENAELPEIDVRLDRGDLLLLYTDGLIEAGGRRSLGVEGLVEA
                     LRHFRGRSAEETVEGLLRTAGNLNGDRPRDDLALVALQIRDPAPDDRPLT"
BASE COUNT          603 a         1901 c         1843 g          739 t
ORIGIN      
        1 agcgcagctg aaacggtaag ggagatcgag gagacccggg accgcctgga cggcgagctc
       61 cgcgcgctcg aggatcgcct tccccgcccg gcggtctgga cgaagaggat cgccggcgtc
      121 gccatcggag gaggcgtcgc cggagccgcg ttctggttcc tggtgcggcg ccggcgagcg
      181 aagcgcgtgc cctccacgtc cgccaacgtt caggcggtcg tgcacgtgct gcccgagggc
      241 ctggccgcgc gcctggagca cgcggtcgag gacggccgct ggcggggcac ggcgatgggg
      301 gtcgccggcg tctggctcgc cctccggctg gccgagctgc gtcagctccg ccggatgaac
      361 cgggccctgc tcgccgcacg ctgagcgggc ccggtccggg atccggctcg cccgctcccg
      421 cgggagggac cggcgcgact atcgttcggg cgtgagcggt tcccggtacg tgctggtcgg
      481 gctgtcccgc atcgccgtcc gggtggccga cgtcctcgcc gcccgcggcg acgaggtcgt
      541 ggtggtcccg gaaccctctg gcggggacac ggcggccgag accctcctgc gccaccgggt
      601 ccgggtgatg gaacccaccc cggaccggga ggaagccctg cgggcggcgg gggtcgacgg
      661 tgccgactgc ttgctcgcgg tctccgacga cgacctggag aacctccacg ccgcgctcgc
      721 ggcgggcgcg gtggcgcccg acgtgccact cgtgatccgc tcgttcgaac cggtcctcgt
      781 ggaccgcctg gcccggagcg tccgcctgcg ccgggcctac agcgtctcgg cgctggcggc
      841 cccggccttc gtcgccgcgg cgctcggcga ggacctcctc gcgacgctcc gcctgggcga
      901 ggacgacatc tcgctctgcc gactggaggt ccggcccggc gcgccgatcg ccggcgccac
      961 cgcggccgag gtgaaggcgg actccggctg cgccgtcgtc gcccatgacg acgggtccgg
     1021 atggcggccc tgcgcgccgg tcgagcggat caccgagggc gagcgcatcc tgatcggcgg
     1081 ccccctgcgc ctggcgctgc tgctggcgaa gcgggtgagc cccgtgctcg ggaaggaccg
     1141 ggcacgcccg gcgccgggtc tcctgcaccg gctccgggac cgccgtgccg aacgcaaggc
     1201 cctccacgcc gggctgcggg ctaacacgct cctccccgcg gccgccgcgg tcctggtcgg
     1261 cctgctgctg atcaccgtga tcgtgttcgc cgtggcgctg cacctcaacc cgatcgacgc
     1321 gatctacttc gcggtcagca cggcgctggg gaacgccacg ctcgaccaga acgaggcgtg
     1381 gctgaaggcc ttcgggatcg gcgccatggt cgccgggggc gccctcctcg gcgtggtctt
     1441 ctcctacctg gccgcactgg cgacggccga gcgcctggag gagcagatgg gccggcgcgc
     1501 ccggcggctg tcgaatcacg tggtcctcgc cggcctcggc acggtcggct accggatcga
     1561 gcacctcctg tccgacctgg agatcccgct ggcggtgatc gagcgctccc ccgacgaccg
     1621 cttcgtggcg acggtcgccc gccgcacgcc tgtggtgatc ggggacgcgc ggctgccgga
     1681 ggacctgatg cgggtcggcg tcgaggacgc ggcgtgcttc gtggcctgca cggaggacga
     1741 gctgacgaac gtcgccgcct gcctgcaggc ccagcggcag aacccgggcc tgaggacggt
     1801 ggcacgcgtg ttcgacgagg agctcggcgg gcggatccag accacgctcg gcatcgacgc
     1861 ggcggtcagt ggcacccgcg ccgcgaccgg cgcgttcgtc tccgccgccg tcgacgaacg
     1921 cgccccgcgg acgctgcatc tgggcggggt ggcgttcgtg gcggtccgct tcgagcccac
     1981 cgtccccctc gacgccgggg agatcgatcg gtggcgaggt cgtggcctgc gcgtgctggc
     2041 cttctcgcgc ggcaacggtc cggtccgcgc cgcctccgag ctcgacggcg gcctcggacc
     2101 gggggacgcc gcgatcgtcg ccgggcccga ggcagccgtc cgggagacgc tcttcggcgc
     2161 ggcccggccg gggatcgctg ataggttgcg gccgtgaccg ccgaccagga ggctcctgcc
     2221 ggcgagcccg tcacccgtcc ccgccgcttc tcgacggccc ggcgggcgaa ggtggcgctg
     2281 caggcggccg cggcggtcct ggccctggtg gcggtcaagc tgatcgtcca ccacttcgat
     2341 ctggagttca tcgagctcag cccgctctac acgagcatcg tcgccggcgg catcttcgtc
     2401 atcggcctgc tcgtcgccgg gaccctcgcc gactacaagg agagcgagcg gatgccggcg
     2461 gagatcgcgg catcactgga gagcatctac gaggactgcc gctcgatcca cgaggggacc
     2521 ccggccttcg acctggcgcg gctgcggagc ctgctcgggc gggtcgtctc gtccctgcgc
     2581 tacgacctgg ccgacccgcg attcacccgg cactgcctcg atgccctgcg cgaggtgtcg
     2641 gcgtcgttcc tggagctcga gcggctcggc gtaccggcca actacgtcgt ccggctccgg
     2701 accgagcagg gctcgatccg gcgcaacgtg ttgcgcgtct accacatcca gcggaccgac
     2761 ttcctcccgt ccgggtacct cctgatccag acgatggtcg tgctgatcct ggccgcgctc
     2821 gtgttcacga agatcgagcc gctgtcgctc gccatcgtgg tgctcgtgtt cctcacctac
     2881 ttcttcgtgt acctcgagcg gctgctccgg ctgctcgatc ggcccttccg gctggatgag
     2941 cgcacccccg acgacgtgag cctgttcctg ctggacgcct tcgcccatca tcttcgcggg
     3001 gggtttgggg aagtagctcc ccccctggag cgcgagacgc cagcgcctcc ggcgagcgga
     3061 ccccccaagg aagagagccc cagggaggcc ggtggccggg aggagcggtt cggcgtaccc
     3121 tgaggctcgg ggaggcatcg cccgcaatcc gtccgggccg cgaccactcc cgggaggatc
     3181 gggcgacgcg tatgcgcagg aagtccctgc tcgtcacctt ctccccgcgc tcacggatct
     3241 ggttcgggat cgtcctgccg gtgctcgccg tggccggggt gacggccgcg ctgtgggcgt
     3301 cgggtccgta cccggcggtg atccccgggc tcctcctgct gatccccgtg actgcggcgg
     3361 ggctgctcgg cgggttcgtc gcgggcatcg tcgtcacggt ctcggcgtcc ctcgggttcc
     3421 tgctgcttct gcccccgatc gggcgcctcg ccctcggggg gcggttgccc aacgtggagg
     3481 gccccggtta ctcgaccgac gtggtggtga tgctggcctt cgtcggcgtc tcgctggcac
     3541 tggtccgcga gacctcctgg gaacgccacc agcgccggcg ggccgagacc gagcgggagc
     3601 agctcgcctt cctggcgacc gccagcgagt ggctgcacgg gacccttgac gcgcgcgacg
     3661 cgctggagcg cgtcgccaac ctcgcggtcc cgcggctcgc ggacgggtgc acgatcgaga
     3721 tcgtgcgtga cgacggcacg ctcgacacgg ttgccatggc cttctccgac ccggagaagg
     3781 ccgcgctggc tcgccgaatg cgtgagcgct tcccgccgcg gcctggtgat ccgggcggcg
     3841 tctgggaggc gatccgggag gagcgcgcca ccgtggtccc tcgcgtcgcc cgccggatcc
     3901 tgcggcagtt cgccccgtcg gaggagcagt tcgacctcgc ctcgcggctc aggccacggt
     3961 cgatcatcgt cgcgccgctc gtggcgcagg ggccaccgat cggcgcggta acgctcgtga
     4021 tggccgaatc cggccggcgg taccttcgcg gcgacctgac cttcgtccag gacctcgcgc
     4081 tgcgcgccgc gacggccgtc gagaacgctc gccgctacac agagcgcagc cggatcgcca
     4141 gcacgctcca gcgggccctg ctccccgagg ccctccccga gatcccgttc gcgcgcgtgg
     4201 cgcaccgcta ccaggcggcg gccgaccgcg tgggcgggga cttctacgac gccatccgca
     4261 gcggcgaccg gagctggtcc gtcttcatcg gcgacgtccg cggacggggc gtcgaggccg
     4321 cctcgctcac agcgctcgcc cgtcacacga tccggacggc ggccctgctc gagcgcggcc
     4381 cggccgcggc gctctcgatc ctgaacgagg ccatcctgcg ccgcggtgac ccggagagct
     4441 tctgcacact cgcctacgtc tgcctctatc cgacggcctc gggtgtggtg gcccgcatcg
     4501 cctgcggcgg ccatccgccg ccgctggtgc tccgcggcca aggggcggtc gaggtggccg
     4561 gcgcgacggg gaccctgctc ggggtgttcg agaacgccga gctcccggag atcgacgtcc
     4621 gcctggaccg cggcgacctg ctcctgctct acaccgacgg cctgatcgag gccgggggac
     4681 gccgcagcct gggggtggag ggcctggtgg aggcactccg ccacttccgc ggccgctccg
     4741 cggaggagac cgtggagggt ctcctcagga ccgccggcaa cctcaacggc gaccggcctc
     4801 gcgacgacct ggccctcgtc gcgctacaga tacgcgaccc ggcgccggac gaccggcctc
     4861 tgacctaggc atccgcgcgc ggaacacgat ccggacgcgg gtcccgggac ggtcctcggt
     4921 cccgcgctcg atctcgaact cgtcggccac ggcccgcatc aacccgatcc cgtagccgga
     4981 cgcgcccggg acctccggca cgccgttcgc acggaacacg ccgaagtcct cgacccggat
     5041 ctcggccagc ccgcccatcg atgcccacgt caccaccagg ttggcg
//
LOCUS       DASPWO010000441         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1164887, whole genome shotgun sequence.
ACCESSION   DASPWO010000441 DASPWO010000000
VERSION     DASPWO010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164887"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VEO00_10040"
     CDS             complement(<1..738)
                     /locus_tag="VEO00_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYU58373.1"
                     /translation="MRVLLIVNLTASSVTARRRVEIQRILGADHKLEVVETSRRGHAA
                     RLARAAALDGVDVVAVLAGDGTLNEAADGLAGTLTALAPLPGGSTNVFARTIGVAYDP
                     VEAARHLVASLRSFTFRRIGLGSASGRRFMFHLGIGFDAAVIGQVERRSFLKRHFAHP
                     AYVLAAVSTWFRHYDHSRPRFRVSVPDGASGNEEVGDSGFAIVSNTSPYTYLGRRPIV
                     AAPAAGLDDPLTLTMFRALEVPLILAAA"
     gene            775..1491
                     /locus_tag="VEO00_10045"
     CDS             775..1491
                     /locus_tag="VEO00_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HYU58374.1"
                     /translation="MPRRDTLAGLTREITACRACPRLVDWRERVARDKRAAFRDEEYW
                     GRPIPGFGDPGARLLVVGLAPAAHGGNRTGRIFTGDRSGDWLFGSLHRTGYANQPTST
                     SADDGLRLRDAYVAAAVRCAPPANKPTPEERDRCLPYLARELDLLEEVRVIVVLGGFA
                     YDAVWRTLTLAGVELPRPRPRFGHGTEAPTPRATVLCSYHPSQQNTFTGKLTEPMLDA
                     VFERARELSRAYRARSSPIA"
     gene            complement(1461..>2299)
                     /locus_tag="VEO00_10050"
     CDS             complement(1461..>2299)
                     /locus_tag="VEO00_10050"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYU58375.1"
                     /translation="GHARRLARADPPIACRFVPEPWRTLLTLAAGLVTGVLSGMFGVG
                     GAVVSTPAIRALGATPLEGVGSTLPSIIPSAISGSLRYRREGLIVGRVVRWTVAFGLA
                     ASVGGALLAEAVPGRGHLLMLMTAALLAFTAYRTAAPRRASVEEPSTTAGNVRRDERW
                     RLAAIGVAAGGLSGLLGVGGGILMVPAFASWVRLTIKEAVATSLACVGVLAVPGTITH
                     ALLGHVVWAFAIPLSIAVIPGARLGAQLAIRATDRGLRLAVGVALGTIAIGYAIGELL
                     AL"
BASE COUNT          349 a          852 c          831 g          267 t
ORIGIN      
        1 cgccgccgcc aggatcagcg ggacctcgag cgcccggaac atcgtgagcg tcaaggggtc
       61 gtcgaggccg gcggccggcg ccgccacgat cggccggcgc cccaggtagg tgtacgggct
      121 ggtgttggac acgatggcga atcccgagtc gcccacctcc tcgttgccgc tcgctccgtc
      181 gggcaccgat acccggaacc gtgggcgcga gtggtcgtag tggcggaacc aggtactgac
      241 cgcggccagg acgtaagccg ggtgcgcgaa gtgccgcttg aggaacgagc gccgctcgac
      301 ctgcccgatg accgcggcgt cgaagccgat cccgaggtgg aacatgaagc ggcgcccgct
      361 ggccgagccc agcccgatgc gacggaacgt gaagctccgc agcgacgcca ccagatggcg
      421 cgccgcctcc accgggtcgt acgccacccc gatggtccgg gcgaagacgt tggtcgaccc
      481 acccgggagg ggagcgagcg cggtcaaagt gccggccagg ccgtcggcgg cctcgttgag
      541 tgtgccgtcg ccggcgagca ccgcgaccac gtcgaccccg tcgagggcgg cggcgcgcgc
      601 gaggcgggcg gcgtggcccc ggcgtgacgt ctcgaccacc tcgagcttgt ggtcggcgcc
      661 gaggatgcgc tggatctcga cccggcgccg ggccgtgacc gacgacgcgg tcaggttgac
      721 gatgagcagg acgcgcacgc acgaagggta gcggcccacc cgtagcatcc gaccgtgccc
      781 cgccgcgaca cccttgccgg actcacgcgc gagatcaccg cgtgccgggc gtgcccacgc
      841 ctcgtcgact ggcgcgagcg ggtcgcgcgc gacaagcgcg cggcatttcg ggacgaggag
      901 tactggggcc ggcccatccc cgggttcggc gaccccgggg cgcgcctgct ggtggtcggc
      961 ctggccccgg ccgctcacgg cggcaaccgc accgggcgca tcttcaccgg cgatcgttcg
     1021 ggtgactggc tgttcgggtc actgcaccgg accggttacg ccaaccagcc gacctccacc
     1081 tccgccgacg acgggctccg cctccgcgac gcctacgtgg ccgcggccgt gcgctgcgcc
     1141 ccgccggcca acaagccgac accggaggaa cgtgaccgct gcctcccgta cctcgcccgc
     1201 gagctcgatc tcctcgagga ggtccgggtg atcgtggtgc tgggtggctt cgcctacgac
     1261 gcagtgtggc gcacgctcac gctcgcgggc gtcgagctcc cccgcccccg gccccgattc
     1321 ggccacggga ccgaggcccc gacaccgcgg gcgacggtgc tgtgctcgta ccacccgagt
     1381 cagcagaaca ccttcaccgg caagctcacc gagcccatgc tcgacgccgt gttcgaacga
     1441 gcacgggagc tgtcgagggc ctacagggcg aggagctcgc cgatcgcgta gccgatcgcg
     1501 atcgttccca gtgccacccc caccgccagt cgcaatcccc ggtccgtggc gcggatcgcc
     1561 agctgcgcgc ccagacgagc gccggggatg accgcgatcg aaagcgggat cgcgaacgcc
     1621 cagacgacgt gcccgaggag cgcatgcgtg atcgtccccg gcaccgcgag tacccccacg
     1681 cacgcgagcg aggtcgcgac cgcttccttg atggtgagcc gaacccagct cgcgaacgcc
     1741 gggaccatca ggatccctcc gccgacgccg agtaggccgc tgaggccgcc ggcggccacg
     1801 ccgatggcag ccagccgcca gcgctcgtcg cgtcggacgt tccccgcagt ggtcgacggc
     1861 tcctcgacgg acgcgcggcg gggcgcggcg gtgcgatagg cggtgaacgc gagcagggcg
     1921 gcggtcatga gcatcaggag gtgaccgcgg ccgggcaccg cctcggccag cagcgcgcca
     1981 ccgacggacg cagccaggcc gaacgcgacg gtccagcgga ccacgcgtcc cacgatcagg
     2041 ccttcgcggc ggtagcgcag cgatccgctg atcgccgacg gaatgatcga cgggagcgtc
     2101 gagccgacac cctcgagcgg cgtcgcgccg agcgcgcgga tcgccggtgt cgagacgacc
     2161 gcgccgccga cgccgaacat tcccgacaag actccggtca cgagcccggc ggccagggtg
     2221 agcagggtcc gccagggctc gggcacgaat cggcaggcta tcggcgggtc ggcccgggct
     2281 agcctgcggg cgtgcccag
//
LOCUS       DASPWO010000442         5249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_486044, whole genome shotgun sequence.
ACCESSION   DASPWO010000442 DASPWO010000000
VERSION     DASPWO010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486044"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VEO00_10055"
     CDS             <1..817
                     /locus_tag="VEO00_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HYU58376.1"
                     /translation="AVVARAAGRTLRVFVPPDGEPAVLRRLKELEADVVTCVREPRER
                     GDPTYRALRRALDDGALPFTCQGPENGLTVEGGETLAWEIVSDLRAADAALDRVFVQV
                     GGGALASSVVQGFEEAVELDALPCAPRVHAVQTRGAFPLQRAYERVARRIADGETAVE
                     ALRYAARHRGEFMWPWEEDPRSVARGILDDETYDWRAVVEGMLATGGSPVVVGEDVLR
                     GANDLAVRSTGVDVDPTGSAGLAGLISLRRAGEIEPGERVAVLFTGIRRDEGG"
     gene            820..1773
                     /gene="pyrB"
                     /locus_tag="VEO00_10060"
     CDS             820..1773
                     /gene="pyrB"
                     /locus_tag="VEO00_10060"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HYU58377.1"
                     /translation="MRSFAGRDILSLKGFEREEYFHVFEVAESLKHHATERRNGDLLA
                     SKTLVTAFYQPSTRTRLATEGAMHRLGGHVVGFSDAKMTRAGDFYQESIKDTVHMLEY
                     YGDVIAMRHFQQGAPAEAARWASVPVINCGDGWGEHPTQVLTDLYTTWREKGRIDGLT
                     FLLVGDMRMRTMHSILYSFSQFDAKAFVVSPEEMSLLPEFTAELDDLSVQYEVVDGVE
                     KAIAESDVIYMEPVVQADYTRSRDERDAEVGRTPAAYRVTRELLRDRAKGDAIVLHSL
                     PRMDELPEDVDATRHARYWAEAFNGVVMRMALLALVLGAVE"
     gene            1781..2731
                     /gene="arcC"
                     /locus_tag="VEO00_10065"
     CDS             1781..2731
                     /gene="arcC"
                     /locus_tag="VEO00_10065"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HYU58378.1"
                     /translation="MARRTAVIAIGGNSLIKDRYHQTVRDQYIAAGETCWHIAGMIQA
                     GWEVAVTHGNGPQVGFIMRRSELAAHELHEVPLDAAGADTQGAIGYALQQNLWNDFRR
                     LGMDKQVVTVVTQTEVAADDPAFQHPSKPIGSFMDEATALRRRDEDGWAVFEDAGRGW
                     RRVVASPKPLSIIEVDAVRSLLDEGFVVISVGGGGIPVVRAPEGDLQGAAAVIDKDLA
                     SSLLATEIGAELFLISTAVERVSLAFGTPEERPIDRITLSEAKAYLAEGSHFAAGSMA
                     PKIEAVIGYLERGGREAIITDPENVERALAGETGTHVVRD"
     gene            complement(2750..3178)
                     /gene="rplU"
                     /locus_tag="VEO00_10070"
     CDS             complement(2750..3178)
                     /gene="rplU"
                     /locus_tag="VEO00_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HYU58379.1"
                     /translation="MYAVIRVGGKQQRVKQGDVIEAEYQKGETPGAELTFKPLLVVDD
                     DGTTHHGKDLEKALVTAKVVGEGKGDKVKVVKYRPKTRYSRHGGHRQMLSLLEISEVR
                     IGAPAKKAAAKAAPKGATKAEEPADAEPAEADDATADSEA"
     gene            complement(3273..>5249)
                     /locus_tag="VEO00_10075"
     CDS             complement(3273..>5249)
                     /locus_tag="VEO00_10075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HYU58380.1"
                     /translation="RRRRRGGRGRGGRGGGGAARTTDETTPEGADPETPSGDERKKAA
                     PRAPRTRAPRARRPAEPAQGEAGPDEETEAARPAPARRRTRSTRTKRSSESGEPAEPE
                     PTPDASAADAAVEPGEAEAATAARRRRRRGGRGRRRPGTTSAATGEGTPGGLAEAALE
                     ELPTEADRQREEALPDEAAEAAPAPKRTRARRGSKAAAKAEPKEPSEEKAERVPSTRR
                     RRAEPGARRRRTTGTRRRREPPLLVHRRTDKRMLITEIGERDQIAVLEDRELVEHYVT
                     RAGARSMVGNIYVGRVQNVLPGMEAAFIDVGRGRNGVLYAGEVNYDEEVEEGSQRIEE
                     VLKNGQSVLVQVTKDPMGGKGARLTAQISLPGRFLVFVPNEQVFGISRRLPDAERTRL
                     RDILKPIRPKDGGVIARTAAAGATAEDLTADLERLRATWEDVAKRSKRAKPPKTIYEE
                     PELTVRVVRDLFTEQEFEELITDSPTVFENVTSYLSAVAPDLVQKVRMHDGTLPLMEE
                     FHVAEQIRKALERKVWLPSGGYLVIDRTEAMTVVDVNTGKHVGKTNLEETVTKTNLEA
                     ADEIARQLRLRDIGGIIICDFIDMLLAQNQRKVIKAMKEALARDRTRSQVFEIGPLGL
                     LELTRKKVSAGLVEAFSETCPTCEGRGILLTHEI"
BASE COUNT          656 a         1893 c         1887 g          813 t
ORIGIN      
        1 ggcggtggtc gcgcgcgccg ccggccgcac gctgcgcgtg ttcgtcccgc ccgacggcga
       61 accggcggtc ctgcgccggc tgaaggagct cgaggccgac gtcgtcacct gcgtgcggga
      121 gccccgcgag cgcggcgacc cgacctaccg cgcgctgcgc cgggccctgg atgacggcgc
      181 gctgcccttc acctgccagg ggccggagaa cggtctgacc gtcgagggcg gcgagaccct
      241 ggcgtgggag atcgtctcgg acctccgcgc cgcggatgcg gccctcgacc gcgtgttcgt
      301 gcaggtcggg ggcggcgcgc tggccagctc ggtggtgcag ggattcgagg aggccgtcga
      361 actggacgcg ctgccgtgtg ctccccgcgt gcacgccgtg cagacccgcg gcgcgttccc
      421 gctccagcgc gcctacgaac gcgtcgcacg gcgcatcgcg gacggcgaga ccgccgtgga
      481 ggcgcttcgg tacgcggcca ggcaccgcgg cgagttcatg tggccgtggg aggaggaccc
      541 gcgcagcgtg gcgcgcggca tcctggacga cgagacgtac gactggcggg ccgtggtgga
      601 ggggatgctg gccaccggcg ggtccccggt cgtggtgggc gaggacgtgc tgcgcggggc
      661 gaacgacctc gccgtccgat cgaccggagt ggatgtcgac ccgaccggct cggccggact
      721 ggcggggctg atctcgctcc gccgcgccgg cgagatcgag cccggcgagc gcgtggcggt
      781 cctgttcacc ggcatccgga gagacgaggg gggttgacga tgaggagctt cgcgggacgc
      841 gacatcctgt cgctgaaggg attcgagcgc gaggagtact tccacgtctt cgaggtggcg
      901 gagtcgttga agcaccacgc caccgagcgg cggaacggcg acctgctggc gtccaagacg
      961 ctggtcacgg cgttctacca gccgagcacg cggacgcggc tggccaccga gggcgcgatg
     1021 caccgcctcg gcggccacgt ggtcgggttc tccgacgcga agatgacccg cgccggcgac
     1081 ttctaccagg agtcgatcaa ggacaccgtg cacatgctcg agtactacgg cgacgtcatc
     1141 gccatgcgcc acttccagca gggcgcgccg gccgaggcgg cacggtgggc ctcggtcccg
     1201 gtgatcaact gcggcgacgg ctggggcgag cacccgacgc aggtcctgac cgacctgtac
     1261 accacctggc gcgagaaggg ccgcatcgac ggcctgacgt tcctgctggt cggcgacatg
     1321 cggatgcgca cgatgcactc gatcctctac tcgttctccc agttcgacgc gaaggcgttc
     1381 gtcgtctcgc cggaggagat gtcgctgctg ccggagttca ccgccgagct cgacgacctg
     1441 tccgtgcagt acgaggtggt ggacggcgtc gagaaggcca tcgccgagag cgacgtgatc
     1501 tacatggaac ccgtggtgca ggcggactac acgaggtcgc gcgacgagcg cgatgcggag
     1561 gtcgggcgaa cgcctgccgc ctaccgggtc acgcgcgagc tgctccgaga ccgggcgaag
     1621 ggcgacgcga tcgtgctgca ctcgctgccg cgcatggacg agctccccga ggacgtcgac
     1681 gccacgcgcc acgcccgcta ctgggccgag gcgttcaacg gggtggtgat gcgcatggcg
     1741 ctcctggcgc tcgtcctcgg cgccgtcgag tagcggttcg gtggcgcggc gcaccgcggt
     1801 catcgcgatc ggcggcaact cgctgatcaa ggaccgctac caccagaccg tccgcgacca
     1861 gtacatcgcc gcgggcgaga cctgctggca catcgctggc atgatccagg ccgggtggga
     1921 ggtggccgtc acccacggca acggcccgca ggtggggttc atcatgcggc gctccgagct
     1981 cgccgcgcac gagctgcacg aggtcccgct cgacgccgcc ggcgccgaca cgcagggcgc
     2041 gatcggctac gcgctgcagc agaacctgtg gaacgacttc cgccgactgg ggatggacaa
     2101 gcaggtggtc accgtcgtca cccagacgga ggtggccgcc gacgaccccg cgttccagca
     2161 cccgtcgaag ccgatcgggt cgttcatgga cgaggcgacc gcgctgcgcc ggagggacga
     2221 ggacggctgg gccgtgttcg aggacgccgg gcggggctgg cggcgagtgg tggcgtcgcc
     2281 gaagccgctc tcgatcatcg aggtggacgc ggtgcgctcg ctgctcgacg agggcttcgt
     2341 ggtcatctcc gttggtggcg gagggatccc ggtggtccgc gcccccgagg gcgacctgca
     2401 gggcgctgcg gcagtgatcg acaaggacct cgcctcgtcc ctgctcgcga cggagatcgg
     2461 cgcggaactg ttcctgatca gcacggccgt cgagcgggtc tcgctcgcct tcggcacgcc
     2521 ggaggaacgc ccgatcgacc ggatcacgct gtcggaggcg aaggcgtacc tggcggaggg
     2581 gtcgcacttc gccgccggaa gcatggcccc gaagatcgaa gccgtgatcg gctacctgga
     2641 gcgcggcggc cgcgaggcga tcatcaccga cccggagaac gtggagcgcg ccctggccgg
     2701 cgagaccggc acccacgtgg tccgggactg actcccaaac cgcagcaggt caggcctcgg
     2761 agtcggcggt cgcgtcgtcc gcctcggccg gctccgcgtc ggcgggctcc tcggccttgg
     2821 tcgcaccttt cggcgccgcc ttcgcggcgg ccttcttcgc cggagcgccg atcctcacct
     2881 cgctgatctc gaggagcgag agcatctgcc ggtggccgcc gtggcggctg tagcgggttt
     2941 tcgggcggta cttgacgacc ttgaccttgt cgcccttgcc ctccccgacc accttcgccg
     3001 tgacgagcgc cttctccagg tccttcccgt ggtgcgtggt cccgtcgtcg tccaccacga
     3061 gcagcggctt gaacgtcagc tccgcgccgg gcgtctcgcc cttctggtac tcggcctcga
     3121 tcacgtctcc ctgcttcacg cgctgctgct tcccgccgac cctgatcacc gcgtacatgt
     3181 cgtcctcctc cccttggggg gttcgctcgc cgtatttggt caggtcgatc cctccacagg
     3241 gcgagctacc cccccaaacc cccccgtcgc cctcagatct cgtgggtcag gagtatgccc
     3301 cggccctcgc aggtcgggca ggtctcgctg aacgcctcga ccaggccggc cgagaccttc
     3361 ttccgcgtca gctcgagcag cccgaggggg ccgatctcga acacctggga gcgggtccgg
     3421 tcccgcgcca acgcctcctt catcgccttg atgaccttgc gctggttctg cgccagcagc
     3481 atgtcgatga agtcgcagat gatgatcccg ccgatatcgc gcagccgcag ctggcgggcg
     3541 atctcgtcgg cggcctcgag gttcgtcttg gtcaccgtct cctcgaggtt cgtcttgccg
     3601 acgtgcttgc cggtgttcac gtcgacgacg gtcatcgcct cggtgcggtc gatgaccagg
     3661 tagcccccgg acgggagcca caccttgcgc tccagagcct tgcggatctg ctcggcgaca
     3721 tggaactcct ccatcagcgg cagcgtgccg tcgtgcatcc gtaccttctg caccaggtcg
     3781 ggcgcgaccg cgctcaagta ggaggtgaca ttctcgaaca ccgtcggcga gtccgtgatc
     3841 agctcctcga actcctgctc cgtgaacagg tcgcggacca cccgcaccgt cagctcgggc
     3901 tcctcgtaga tcgtcttcgg cggcttggcc cgcttggagc gcttcgccac gtcctcccag
     3961 gtcgcccgga ggcgctcgag gtcggccgtg aggtcctcgg ccgtggcgcc ggcggcggcc
     4021 gtccgcgcga tcacgccccc gtccttcggc cggatcggct tcaggatgtc acgcaggcgg
     4081 gtgcgctccg catccggcag ccggcggctg atcccgaaca cctgctcgtt cggcacgaac
     4141 accaggaagc ggccggggag gctgatctgc gccgtcagcc gagcgccctt gccgcccatc
     4201 gggtccttgg tcacctggac caggaccgac tgaccgttct tcagcacctc ctcgatgcgc
     4261 tgcgagccct cctcgacctc ctcgtcgtag ttgacctcgc cggcatagag gacgccgttg
     4321 cgcccgcggc cgacgtcgat gaaggccgcc tccatgccgg gcagcacgtt ctggacgcgt
     4381 ccgacgtaga tgttgccgac catcgagcgg gccccggccc gggtgacgta gtgctcgacc
     4441 agctcgcggt cctcgagcac ggcgatctgg tcgcgctctc cgatctcggt gatcagcatc
     4501 cgcttgtcgg tgcggcggtg gaccaggagc ggtggctcgc ggcggcggcg ggtacccgtg
     4561 gtccgcctgc ggcgtgcccc cggctcggcg cggcggcggc gcgtggaggg cacgcgctcg
     4621 gccttctcct cgctgggctc cttgggctcc gccttggcgg ccgccttcga gccacgccgg
     4681 gcgcgggtcc gcttcggggc gggcgctgcc tcggcggcct cgtcgggcag cgcctcctcg
     4741 cgctggcggt cggcctcggt cggcagctcc tccagggcgg cctcggccag gcctcccggc
     4801 gtcccctcgc cggtcgcagc ggacgtggtg cccggccggc gccgtccccg gccgccgcgc
     4861 cgtcgccgcc gcctcgcggc tgtcgcggct tcggcctccc ccggttcgac ggcggcatcc
     4921 gcagccgagg cgtcgggggt cggctcgggc tctgctggct cgccggactc ggacgaccgc
     4981 ttcgtgcggg tggacctcgt ccggcgccgg gccggcgcgg gccgcgcagc ctcggtctcc
     5041 tcgtcgggcc ccgcctcgcc ctgcgccggc tcggcgggcc gccgggcgcg gggggcgcgg
     5101 gtgcggggcg cgcgaggcgc ggccttcttc cgctcgtctc cggacggagt ctccggatca
     5161 gcgccctccg gtgtggtctc gtcggtggtc cgtgcggcgc ctccgcctcc ccggccaccc
     5221 cgtccgcgac cgccgcgacg acggcgccg
//
LOCUS       DASPWO010000443         4120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_234111, whole genome shotgun sequence.
ACCESSION   DASPWO010000443 DASPWO010000000
VERSION     DASPWO010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234111"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VEO00_10080"
     CDS             complement(<1..416)
                     /locus_tag="VEO00_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYU58381.1"
                     /translation="MRGPRTLAGRLFLGVGVSVVALLAVLGMVLDRILEADLVSALTE
                     RLEAQARTVAVALPPGVTGLQADVSRLGGAGGVRITVIRADGVVLADSERDPATLENH
                     RDRPEVREALAGRVGVAVRRSESTGEEYRYVALPVR"
     gene            959..2011
                     /locus_tag="VEO00_10085"
     CDS             959..2011
                     /locus_tag="VEO00_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYU58382.1"
                     /translation="MRRKWLALAALVAAFALVAAACGGNNDTSGGGNGDAARAPDCLN
                     FADLYALIGPESEGFDNWSDAEALAQQVDGLDSYPDAPLTITAPGEESGTYDSFIELA
                     LAGVAETRLESGDITEDQVETTRPDYQASPNDNVIIEGIAGSEASLGWVGFSFYEQNR
                     DVVRAIEIDGGDGCVEPTVETIGDGSYPISRSLYIYVNTAKAADNPALQAFVDFYLTD
                     AGIRESVSEVGYVTLPDDRIAATQATWESEGPGTDTSGDLSGELFMSGSSTVEPISAL
                     VAELFNADHPDVAINVEGPGTGDGFELFCNGETDISDASRQIKDEEAAACADTGIEYV
                     ELEVGIDGISVLTQAG"
     gene            2152..3093
                     /gene="pstC"
                     /locus_tag="VEO00_10090"
     CDS             2152..3093
                     /gene="pstC"
                     /locus_tag="VEO00_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HYU58383.1"
                     /translation="MSVATGSGGPGLTLGDLRGSRSRRNREAAIRWSFFGAAGLSLVI
                     NALIVITLFGKAWDFVSSVDLSALWSNGWFPRRGLFDLKTVLAGTLIVAVTAMIVAAP
                     LGLGAAMYLAEYASPRRRRMLKPILEVLAGVPSVVLGYFALTWISPNVTQKLFGSSLF
                     NLAAAGLGVGVLITPLVASVAEDAMYAVPRSLREASYGLGATKRTTTLRVVFPAAVSG
                     IVAALILGFSRAIGETLIVAIAGGGTGGSAFSVNPFEQGQTMTAAMTALATGSDQVRG
                     AGGAFESLFFLGLLLFLMTLTLNVVSERFVRRVRQRY"
     gene            3093..4043
                     /gene="pstA"
                     /locus_tag="VEO00_10095"
     CDS             3093..4043
                     /gene="pstA"
                     /locus_tag="VEO00_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HYU58384.1"
                     /translation="MARETALTRPMPTPAELVRQAILGRRRDVRGAVFQWTLLLAMLL
                     ALAVLTVLLVDIVQGALPVIRDRGVKFLGQNISRIPDRAGVAQGIKGSLIITGTVMLL
                     AFPIGIGAAIYLEEYARDTRLTRIINANIRNLAGVPSIVYGLLGIALFVTFAPLARLT
                     GGRSQLTAGLTMSILVLPIVVITAAEAIRAVPESIREAGFGVGATRWEVIRSHVLPYA
                     APGIFTGAVLTIARAFGEAAPLILIGAATGFLSTGTASFAEQLRGPYTALPVQVYAYA
                     KQNLRTFENVTAAAALALLAVLLLVNLTAILLRNRYERRW"
BASE COUNT          588 a         1458 c         1460 g          614 t
ORIGIN      
        1 tcccggaccg ggagcgcgac gtaccgatac tcctcgccgg tggactcgct gcgacgcacg
       61 gccacgccga cccggccggc cagcgcctcg cgcacctccg gacggtcgcg gtggttctcg
      121 agcgtcgccg ggtcgcgctc cgagtcggcg agcaccaccc cgtcggccct gatcaccgtg
      181 atcctgacgc cgccggcgcc gccgagccgc gacacgtccg cctgcaggcc ggtcacgccc
      241 ggcgggagcg cgacggcaac ggtgcgggcc tgcgcctcga gccgctcggt gagggcggag
      301 accaggtcgg cttccaggat gcggtcgagg accatcccga gcaccgcgag gagggccacc
      361 acggacacgc ccacgccaag gaacagccgt ccggcgagcg tgcgcggccc cctcatgtgt
      421 cgcggcaagt ctacgggcga gaagcgagcg gcccgcgagg tgttcattcc gtggtcgccg
      481 acgcgtcgcg gagcggtcga agcgggtcga gacactccgg ccgtggcgaa cgtcgcggag
      541 cgggccgaat ggctcgactg ccccgagtcc ggagctgccg gggccatccg ccgcgggctc
      601 tgcgaggtcc gctacgcgga gatcgacgag tgcgacgggg ggtttggggg agtagctcgc
      661 cctggggaga gatcaccccg gccacgtccg gcgagcgaac cccccaagga gacgagggga
      721 ccgagtagac cgcacccggc ccggccaacg ttcaccgcgc gttcaactcg gagcaagggt
      781 ccagtcacgc ggcggcccta tcgtcgcgcc ggtgacaccg gtggatgccc acccacgccg
      841 ccgtcgcacc tacccgccgc gaccggcggc ttcccaggag gcgattcgga tccctctccc
      901 cgaatcgctt cccgggaaca ccccgttccc gcaccgacgc gaaggaggaa ccgtgcccat
      961 gagacggaag tggctcgcgc tcgcggcgct cgtcgcggcg ttcgcgctcg tcgccgcggc
     1021 ctgcggcggc aacaacgaca cgtctggcgg ggggaacggc gacgcggcgc gcgcgcccga
     1081 ctgcctgaac ttcgccgacc tctacgccct gatcggcccc gagtctgagg gcttcgacaa
     1141 ctggtccgac gccgaggcgc tggcgcagca ggttgacggg ctcgacagct acccggacgc
     1201 gccgctgacg atcaccgcgc ccggtgagga gtccgggacg tacgactcgt tcatcgagct
     1261 cgcgctcgcg ggcgtcgccg agacgcggct cgagtccggc gacatcaccg aggatcaggt
     1321 cgagacgacc cgtcccgact accaggcgtc gccgaacgac aacgtgatca tcgagggcat
     1381 cgccgggtcc gaggcgtcac tcgggtgggt cgggttctcg ttctacgagc agaaccggga
     1441 cgtggtccgg gcgatcgaga tcgacggagg cgacggctgt gtcgaaccga ccgtggagac
     1501 catcggcgac gggtcctacc cgatctcccg cagcctctac atctatgtca acacggccaa
     1561 ggcggcggac aaccccgccc tgcaggcgtt cgtcgacttc tacttgaccg acgccggaat
     1621 ccgggaatcg gtcagcgagg tcgggtacgt cacgctcccc gacgaccgga tcgcagcgac
     1681 ccaggccacc tgggagagcg aggggccggg gaccgacacc tccggcgacc tgtccggcga
     1741 gctgttcatg tcggggtcgt cgacggtcga gcccatctcg gcgctcgtcg ccgagctgtt
     1801 caacgccgat caccccgacg tggcgatcaa cgtcgagggg ccggggacgg gcgacggctt
     1861 cgagctcttc tgcaacggcg agacggacat ctccgacgcg tcccgccaga tcaaggacga
     1921 ggaggcggcg gcctgcgcgg acacggggat cgagtacgtc gagctggagg tcgggatcga
     1981 cgggatctcc gtcctgaccc aggcgggctg atcccgccaa gggttcgggg atcccgaggg
     2041 gccggccccc aggggccggc cccgctcgcg tgtggggcca ccagcgcctc gttcacctcc
     2101 cgttcacctg ccctccgtca ggggttcaag acccgctgat agcgtggggc cgtgagcgtg
     2161 gcgacgggct cggggggacc cgggctcacc cttggcgacc tgcgcggtag ccgttcgcgg
     2221 cgcaaccgcg aggccgccat ccggtggtcg ttcttcggag ccgccgggct ctcgctcgtg
     2281 atcaacgcac tgatcgtgat cacgctgttc gggaaggcct gggacttcgt cagctcggtc
     2341 gacctgagcg ctctgtggag caacggctgg tttccccggc gcggcctctt cgacctgaag
     2401 accgtcctgg cgggcacgct gatcgtggcc gtgaccgcca tgatcgtcgc tgctccgctc
     2461 ggcctgggcg cggccatgta cctggccgag tacgcctcgc cccgtcgccg ccggatgctg
     2521 aagccgatcc tggaggtcct ggccggggtg ccgagcgtgg tgctcgggta cttcgcgctg
     2581 acctggatca gcccgaacgt gacccagaag ctcttcggct cctcgctgtt caacctggcg
     2641 gcggcggggc tgggggtggg ggtgctgatc accccgctcg tcgcctcggt cgccgaggac
     2701 gcgatgtacg ccgtcccgag gtccctgcgc gaggcgtcct acgggctggg ggcgaccaag
     2761 cgcaccacca cgttgcgggt cgtcttcccg gcggccgtct cgggcatcgt ggcggcgctg
     2821 atcctggggt tctcgcgcgc catcggcgag acgctgatcg tggcgatcgc cggcggcggg
     2881 accggcggtt cagccttctc ggtgaacccg ttcgagcagg gacagacgat gaccgcggcg
     2941 atgaccgcgc tggccaccgg ctccgaccag gtacggggcg ccggcggagc gttcgagagt
     3001 ctgttcttcc tcgggctgct cctgttcctg atgacgctga ccctgaacgt ggtcagcgag
     3061 cgcttcgtcc gtcgcgttcg gcagcgatac tgatggctcg cgagaccgcc ctcaccaggc
     3121 cgatgcccac cccggccgag ctggtccggc aggccatcct gggccggcgt cgcgatgtcc
     3181 ggggggcggt gttccagtgg accctgctcc tcgcgatgct cctggccctg gcggtgctga
     3241 ccgtgctcct cgttgacatc gtccaggggg ccctgcccgt catccgggat cggggggtga
     3301 agttcctggg gcagaacatc tcccggatcc ccgaccgtgc gggcgtcgcc caggggatca
     3361 aggggtccct gatcatcacc gggacggtca tgctgctggc attcccgatc ggcatcggcg
     3421 ccgcgatcta cctggaggag tatgcccgcg acacccgcct gacccggatc atcaacgcca
     3481 acatccgcaa cctcgccggc gtgccgtcga tcgtgtacgg gttgctcggc atcgccctgt
     3541 tcgtgacgtt cgcgccgctg gcgcgcctga ccggcgggcg aagccagctc accgccggcc
     3601 tgaccatgtc catcctggtg ctgccgatcg tggtgatcac cgcggcggag gccatccgag
     3661 cggtcccgga gagcatccgg gaggccgggt tcggcgtcgg cgcgacgcga tgggaggtga
     3721 tccgcagcca cgtgctgccc tacgccgcgc cggggatctt cacgggcgcg gtgctgacca
     3781 tcgcccgcgc cttcggagag gccgcccccc tgatcctgat cggcgcggcc accggcttcc
     3841 tctcgacggg gacggcaagc ttcgccgaac agctgcgggg cccctacacg gccctgccgg
     3901 tgcaggtgta cgcctacgcc aagcagaacc tgcggacgtt cgagaatgtc acggcggcgg
     3961 ccgccctggc cctgctcgcc gtgttgctgc tcgtcaacct gaccgccatc ttgttgcgca
     4021 atcgctatga acgaaggtgg tgaagagcca atgagcgttg aggtccgtcc cggcgccgcc
     4081 ggcgaggggg gcgtcaaccc ggagaaggcc gcacgtgagc
//
LOCUS       DASPWO010000444         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_913799, whole genome shotgun sequence.
ACCESSION   DASPWO010000444 DASPWO010000000
VERSION     DASPWO010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913799"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VEO00_10100"
     CDS             complement(<1..837)
                     /locus_tag="VEO00_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYU58385.1"
                     /translation="MAGLDPRDAVVVVTGASSGIGWSTAWAFAARGAVVVPTARRLER
                     LENLAFEIRARGGTATPVACDVADRDSIHALAEAVRDLHGRCDVLVNNAGIPGGGHFD
                     DVSEEQIEAVVRTNYLGVLYCTKAFLPLLLEAERGHIVNVASLAGRYALPGSAVYGSA
                     KHAVVAFSESLDYELAARGVRVTTVNPGLVSTERFPHRDAGEGGGIRSRLVMRPERIA
                     REIVRVVERGIAPERSVPRWLAAPQAARLLAPPLFRGAVRRVAAGVVRTTRVDEAVAP
                     IRA"
     gene            complement(845..1621)
                     /locus_tag="VEO00_10105"
     CDS             complement(845..1621)
                     /locus_tag="VEO00_10105"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYU58386.1"
                     /translation="MIDFSLTGKVAVITGGSKGIGKGIALAFADAGADVVLAARGEED
                     LTLAAKEVGGRGRRALAVPTDVSDPAQVQALIDRTVAELGTVDVLVNNAGAAPFMSTL
                     DSMRMEGFRKYFEINFWGAVNAIRAVAPILTGKNAGSVINIASVAAYIASPGLSYYST
                     AKAAIVNLTKTIAFEWAGHRVRVNAIAPGWVESEMNARAREVDAFREGILQQIPLGRW
                     GTPEDVAAVAVFLASDAAAFVTGSVYVVDGGQTVSTLAGA"
     gene            1649..>2451
                     /locus_tag="VEO00_10110"
     CDS             1649..>2451
                     /locus_tag="VEO00_10110"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HYU58387.1"
                     /translation="MCARKAQNPRTHPAAERGADRWTPAPYAPDELLRALVDEGMAGE
                     VSHDRQNVLGKCQRLVEGDVDVTFGLSGMTRYTRDEVVALVDRATRFDAGAGLFEGPT
                     IIDPVPVLEMLEAMGDRLALACERGESMVFATGHPVGLLQLYMALAREVAARGVKLLR
                     PGEGEVWREWGHRRTVRYLGGVAVLTDRASSFHTHSPEAMRRMLADARPDLVVGDHGY
                     AGAAIEAGVETLAVTDVNDPAVLVAQSEGRVAVVVTMDDNVLPEKYWPC"
BASE COUNT          346 a          896 c          885 g          324 t
ORIGIN      
        1 cgcgcggatc ggggccaccg cctcgtcgac gcgggtcgtg cgcacgaccc cggcggcgac
       61 ccggcggacc gccccgcgga acaggggcgg ggcgagcagg cgggcggcct ggggcgcggc
      121 gagccagcgg gggaccgagc gctccggggc gatgccgcgc tcgaccacgc gcacgatctc
      181 ccgggcgatc cgctcgggcc gcatcacgag ccgggagcgg atgccgccgc cctcgcccgc
      241 gtcgcggtgg gggaaccgct cggtcgagac gagcccgggg ttgacggtgg tcacccgcac
      301 cccgcgcgcc gccagctcgt agtcgagcga ttcggagaag gccaccaccg cgtgcttcgc
      361 cgagccgtac acggcggagc ccggcaacgc gtagcgcccg gcgagcgagg cgacgttcac
      421 gatgtgcccg cgctccgcct cgaggagcag cggcaggaag gccttcgtgc agtagagcac
      481 ccctaggtag ttggttcgca cgaccgcctc gatctgctcc tccgacacgt cgtcgaagtg
      541 ccccccgccg gggatgccgg cgttgttcac cagcacgtca caccgcccgt gcaggtcgcg
      601 gacggcttcg gcgagggcgt ggatcgaatc gcggtcggcg acgtcgcagg cgaccggggt
      661 cgccgtgccg ccccgggcgc ggatctcgaa cgcgaggttc tcgagccgct ccagccgccg
      721 cgccgtgggc acgaccaccg cgccgcgcgc ggcgaacgcc caggcggtcg accagccgat
      781 cccggaggac gcgccggtca cgacgacgac cgcgtctcgc ggatccagtc cggccactgg
      841 cgccctacgc tccggcgagc gtggagacgg tctggccgcc gtcgaccacg tagaccgagc
      901 cggtcacgaa ggctgctgcg tccgaggcga ggaagaccgc cacggcggcg acgtcctcgg
      961 gcgtgcccca gcgcccgagc gggatctgct gcaggatccc ctcgcggaag gcgtccacct
     1021 cgcgcgcccg ggcgttcatc tcgctctcga cccagccggg ggcgatcgca ttgacccgca
     1081 cccggtggcc ggcccactcg aaggcgatcg tcttcgtcag gttcacgatc gccgccttcg
     1141 ccgtggagta gtaggagagg cccgggctgg cgatgtaggc ggcgaccgac gcgatgttga
     1201 tcaccgaccc ggcgttcttg cccgtcagga tgggcgccac ggcccggatc gcgttcaccg
     1261 cgccccagaa gttgatctcg aagtacttgc ggaacccctc catccgcatc gagtcgagcg
     1321 tggacatgaa cggcgcggcg cccgcgttgt tcaccagcac gtccacggtc ccaagctcgg
     1381 ccacggtccg gtcgatcagc gcctgcacct gagcggggtc gctcacgtca gttggcacgg
     1441 cgagggcgcg ccggccgcgc cccccgacct ccttcgccgc gagcgtgagg tcctcctcgc
     1501 cgcgcgccgc gagcaccacg tcggcaccgg cgtcggcgaa ggccagcgcg atgcccttgc
     1561 cgatcccctt gctgccgccg gtgatcaccg cgaccttgcc ggtgagggag aagtcgatca
     1621 tccgagaacc tccgctagca tcgggtcgat gtgcgctagg aaagcacaga acccccgtac
     1681 ccatcctgcc gccgagcgcg gtgccgaccg ctggaccccc gccccgtacg caccggacga
     1741 actgcttcgc gccctcgtgg acgaggggat ggccggcgag gtctcccacg atcgccagaa
     1801 cgtcctcggc aagtgccagc ggctggtgga gggtgacgtc gacgtcacgt tcggcctctc
     1861 cgggatgact cgctacacgc gcgacgaggt tgtggcgctc gtggaccgcg ccacccgatt
     1921 cgacgccggc gccggactct tcgaggggcc aacgatcatc gaccccgtgc ccgtgctgga
     1981 gatgctcgag gccatgggtg accgattggc gctcgcctgc gagcgcggcg agtcgatggt
     2041 gttcgccacc ggacacccgg tcgggctgct gcagctgtac atggccctgg cgcgggaggt
     2101 ggcggcgcgc ggcgtgaagc tcctgcggcc cggcgagggg gaggtctggc gcgaatgggg
     2161 ccaccgccgg accgtgcggt acctgggcgg ggtggcggtg ctgacggacc gggcctcctc
     2221 gttccacacc cacagcccgg aggccatgcg ccggatgctg gccgacgccc gccccgacct
     2281 ggtcgtcggc gatcacggat acgccggcgc cgcgatcgag gccggcgtcg agacgctggc
     2341 cgtcaccgac gtcaacgacc ctgcggtcct cgtggcccag tccgagggac gcgtcgcggt
     2401 ggtggtgacg atggacgaca acgtgctccc ggagaagtac tggccatgct t
//
LOCUS       DASPWO010000445         8916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1165229, whole genome shotgun sequence.
ACCESSION   DASPWO010000445 DASPWO010000000
VERSION     DASPWO010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8916
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165229"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1078
                     /gene="mdh"
                     /locus_tag="VEO00_10115"
     CDS             149..1078
                     /gene="mdh"
                     /locus_tag="VEO00_10115"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HYU58388.1"
                     /translation="MQVTVVGSGFVGATTAMRVAQKGLADEVVMVDIVEGLPQGLSLD
                     MNQSSPVEGFEPVVRGTNDYGDTAGSDVVVITAGLPRQPGMSRMDLLGTNARIMRDVA
                     GNVKATSPNAIIVVVSNPLDEMTFLAAEVTGFPRERVMGMAGVLDSARLRTFIAERLA
                     VSPSLVEAITLGSHGESMVPLPRHATVDGKPLTELVDEATLEELFARTRDAGAEIVGY
                     LKKGSAYYAPSASVARMVNAIAGDTKEVLPVCAWTTGQYGIADVYVGVPARLGRGGVE
                     GIDELDLNDDELAALRGAADGIREKCSDLAKLG"
     gene            complement(1082..1438)
                     /locus_tag="VEO00_10120"
     CDS             complement(1082..1438)
                     /locus_tag="VEO00_10120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU58389.1"
                     /translation="MGVGGRPGRRVPDRRPATSPPRKPRRGCCCGRSSRGPTIGRRSG
                     DRGWIAELGVRPAWRDRGIGVALLRASFARFARLGLREALLSVDSENATGATCLYERV
                     GMRSRFRFDFYEKAIE"
     gene            complement(1659..2366)
                     /locus_tag="VEO00_10125"
     CDS             complement(1659..2366)
                     /locus_tag="VEO00_10125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYU58390.1"
                     /translation="MFDKTARFYDAIYSFKDYPAEAARVQEIVEDRNPGAHTLLDVAC
                     GTGRHLEHLRERFAVEGLDLDPALLAVARERLGPDVPLHVADMVAFDLGRAFDAVTCL
                     FSAIAYARTGENLARAVERLLAHTAPGGVVIVEPFFERSQWRWPEVHATYVDEPDLKI
                     ARMNRTAEPGETIVMEFEYLIGTPKGIERATERHEVGAFTRGDYQAAFRAAGVEPEWT
                     DDGLMEDRGLWIAIKPA"
     gene            2420..3274
                     /locus_tag="VEO00_10130"
     CDS             2420..3274
                     /locus_tag="VEO00_10130"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019153745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HYU58391.1"
                     /translation="MSEVREVPTDQLAAVVRELCMEICYRVPPEMVELMKRAREREES
                     PLGRQILDQLLKNQDIAAEGEYPYCQDTGYTVVFLDIGQNVHVTGGDLYDAIDRGVAE
                     GYTDGYLRGSIVRDPLFDRKNTGDNTPAFVHTRIVPGDQVRIQIDAKGAGSENMGRHA
                     MLKPADGLEGVKHFVLKAVAESGPNACPPGIIGVGVGGNFEMSAVLAKRALMRKVGQP
                     SPDPLYAELEAELYEKCNALGLGPEALGGTQTVLAVHVEGMPTHIASLPVAVNIECHA
                     HRTGVRTI"
     gene            3274..3927
                     /locus_tag="VEO00_10135"
     CDS             3274..3927
                     /locus_tag="VEO00_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HYU58392.1"
                     /translation="MPVEPQGDGRVRISPPLTDDDVRSLEAGTVVLVRGVVYGARDAA
                     HKRMIEDLDAGRDLPFDPAGQIVYYVGPTPERPGNVIGSAGPTTASRMDRWTPTLLER
                     GLKGIIGKGGRGPAVRQELKRHTAVYMAALGGGGALSALTVRSQRVIAYEDLGTEAIR
                     AIEMDDFPAWVVNDAAGRDFYAETIRPWRRNDLLPDDLHLEEAAGKAIEERGFLGGG"
     gene            3943..4752
                     /locus_tag="VEO00_10140"
     CDS             3943..4752
                     /locus_tag="VEO00_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYU58393.1"
                     /translation="MSAGVPFDRAVAFYDRTRSLPPEVSSAVARLLAGELEGRGRALE
                     GRGRALEVGAGSGRITLPLAARGIPLAAVDLSRPMLEQLQRNAGGRAPFPVAVADGLA
                     LPFPSGAFGASLTCHVLHLVAGWQGFLAEVVRVVRPGGVVLFDLGSEPPEIQETKERF
                     AREAGLERVYVGMRDPVVLDRAMAGLGASARLLEPIRFETRQRIRDVLRGYAGAQFSF
                     TWVVEDEAVLRRAARATRAWAAGRFGDPDEQRTFVRTIQWRAYDLPGAAGA"
     gene            complement(4758..5825)
                     /locus_tag="VEO00_10145"
     CDS             complement(4758..5825)
                     /locus_tag="VEO00_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HYU58394.1"
                     /translation="MHRVTLIPGDGIGPEVAAAARRVVEAAGAEIEWDVQAAGATVVE
                     AEGTPLPARVLDSIRERGTALKGPVTTPVGTGFRSVNVALRQELDLFAAVRPSRSLPG
                     VATRHHNVDLVVIRENTEDLYAGIEFEKGSEELARLRTEIAELAGYRIAEDAGVTVKP
                     ISIGGTRRIVRFAFDFAARHGRAKITVGHKANIMKFSDGVFLFAAAGEAAFHPEVAFE
                     QMQVDELAMWLAWQPQELDVLLLPNLYGDIVSDLCAGLVGGPGLAAGANIGDSAAVFE
                     PVHGSAPDIAGKGVANPIAMVLSAALMLRHLGQHAAAGRVERAVQEALAAGDALTPDL
                     GGNGTTTALVEALARRASPRS"
     gene            complement(5937..>8916)
                     /locus_tag="VEO00_10150"
     CDS             complement(5937..>8916)
                     /locus_tag="VEO00_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYU58395.1"
                     /translation="YGRTVNAFAAKMSAQAAAALAGRTDVASVQPVGVVTRANETSVP
                     FIGAPLVWNNLGVRGEGMRVALVDTGIDYTHKDFGGPGTPGAYAGNDPDIIEPGTFPT
                     GKVIGGYDFVGGNYDVLDGDPSNDIPVPDPDPLDVDGHGTHTGGTCCGNGVPGKVGPG
                     VAPKAKLYAVKVWDVGNSSDDVLVAGYEFAVDPNQDGSTNDRANVLSFSGGVDYGTKN
                     SVEAVAAQGVVDLGTVFVASAGNAGNQPSGGSGYIVGTPAVAPGVVAVAASIDQFTAG
                     TIDVNSPATVLPDGGIYVHQDWSGDITTDITGDIFDVRELDPPAAPDGTPVPSDQEYC
                     TSLGGSPLAGKIALIFKGSTGAGDCGGSTKVYNAEVDGAIAVILWSGFGGAPFGLGPS
                     APPLPTIPAVMVSGPDGAALADAASPGAPGSYNTVVVNVTINANPEVIPGFEDLMTDF
                     TSEGPARLTSDLKPDISAPGSDIGSAAVGTGDDKILLSGTSMAAPHVSGVAALLRQLH
                     PTWTPLQIKAALMNQATQAMKNNDTSTPVPATVMGSGRVQAYESAKAKSLASPGSLSF
                     GYQSMKTTTSFQRTLTVENLASVPHKYTVTGSTRYSDFSNPLATLQFAVTGGTWQSTL
                     SFTLNAGTAKQVKVRLTVDPAAITKADDMYGWYYFNHGLDGNVTIQQSVNGSDTLHVP
                     WHIVPEAASNNSMNKSHLDLTGVLPESSLAMNVDGGAGQNYGDLYLLGALDPADPNPA
                     STGEEDISAIGARSFTGPSIDGVPEGIPNGFDPLVGLPWQIFLTNDNFPAEPVEVAVQ
                     TNGIHNTTDTNEIDVLIDVGGDGVFADPGLKADFLAVKTPEPGGVVVVYDLSLPDPFG
                     NPVATYFNEYTLYDGNLTGIVFDADDIGLSDGNPVFSYGVRACTGRYSGDIPDQICDK
                     AEQMSGGTYRAHLNVIDPALQIDDLVCRGPFGGTSCNAANPIVVSAGLAAPGEDPSIL
                     VVFPNNVPGPGTRTIITTET"
BASE COUNT         1242 a         3132 c         3263 g         1279 t
ORIGIN      
        1 gccgccgaca ccatggaggc cgcgatcgcc agtgtgatcc gcaagggcga gaaggtcacc
       61 tacgacctca agcccacccg tgacgacccc accgccgtcg gcaccagcca ggtcgccgag
      121 gccgtgatcg agaagctcgg agcctgacat gcaggtgacg gtggtcggga gcgggttcgt
      181 gggggcgacg acggccatgc gggtggcgca gaaggggctg gccgacgagg tggtgatggt
      241 cgacatcgtc gaggggctgc cacagggact ttcgctggac atgaaccagt cgtcgccggt
      301 cgaggggttc gagccggtcg tgcgcggcac gaacgactac ggcgacacgg cgggctccga
      361 cgtggtggtg atcaccgcgg ggctgccgcg ccagccgggg atgagccgga tggacctgct
      421 cgggacgaac gccaggatca tgcgcgacgt ggccggcaac gtgaaggcga cctcgccgaa
      481 cgcgatcatc gtcgtggtca gcaacccgct cgacgagatg acgttcctgg cggccgaggt
      541 gaccggcttc ccgagggaac gggtgatggg gatggccggc gtgctcgact ccgcccggct
      601 gcgcaccttc atcgccgagc gcctcgcggt ctcgccgtcg ctggtggagg cgatcacgct
      661 gggcagccac ggcgagtcca tggtcccgct gccgcgccac gccaccgtcg acgggaagcc
      721 gctgaccgag ctggtcgacg aggccacgct cgaggagctg ttcgcccgga cccgcgacgc
      781 cggcgccgag atcgtcggct acctgaagaa ggggagcgcg tactacgcgc cgtcggcctc
      841 ggtcgcccgg atggtgaacg ccatcgccgg cgacacgaag gaggtcctgc cggtgtgcgc
      901 ctggaccacc ggccagtacg ggatcgccga cgtctacgtg ggcgtcccgg cgcgcctcgg
      961 ccgcggcggg gtcgagggca tcgatgagct cgacctgaat gacgacgagc tcgccgcgct
     1021 ccggggggcc gccgacggca tccgcgagaa gtgctccgac ctggccaagc tcgggtaatc
     1081 cctactcgat ggccttctcg tagaagtcga atcggaagcg cgagcgcatc ccgacgcgtt
     1141 cgtacaggca ggtcgcgccg gtggcgttct cggagtcgac ggacagcagc gcctctcgca
     1201 ggccgaggcg ggcgaaccgc gcgaacgagg cccgcagcag cgcgaccccg atgccacggt
     1261 cgcgccacgc cggccggacg ccgagctcgg cgatccaccc gcggtccccc gagcgccgcc
     1321 cgatcgtcgg gccccggctc gaacgaccgc agcagcaccc ccggcggggc tttcgcggcg
     1381 ggctcgtcgc cggacggcgg tccgggacac gacggcccgg ccggcctccg actcccacac
     1441 gaacgcgtag ccggccgcgg acccgtcggg ggccaggacc agcaccggat ccctcgccag
     1501 atcgaaccgc gggcggcccc attcgtccgc caggtcctcc tcctggtaat cgacggcgcc
     1561 gaccagcgcc gtgtcggacg ctgcgatcag gtcgaggatc gccgggccgt ccgccggtcg
     1621 gatcggccgg gttccgtggc cggccggcag cgagccgttc acgccggctt gatcgcgatc
     1681 cacaggccgc ggtcctccat cagcccatcg tcggtccact ctggttccac cccggccgcc
     1741 cggaacgccg cctggtagtc gccgcgcgtg aacgcgccca cctcgtggcg ctcggtggcc
     1801 cgctcgatcc ccttcggggt gccgatcagg tactcgaact ccatgacgat ggtctcgccc
     1861 ggctcggcgg tgcggttcat ccgcgcgatc ttgaggtcgg gctcgtcgac gtacgtggcg
     1921 tgcacctccg gccagcgcca ctgcgaccgc tcgaagaacg gttcgacgat cacgacgccg
     1981 ccgggcgcgg tgtgcgcgag gaggcgctcg actgcccggg ccaggttctc gccggtccgg
     2041 gcgtaggcga tcgccgagaa gaggcaggtc acggcgtcga acgcccgacc gagatcgaag
     2101 gcgaccatgt cggcgacgtg gagcggcacg tccggcccca ggcgctcccg ggccaccgcc
     2161 agaagggcgg gatcgaggtc cagcccctca accgcgaatc gctctcgcag gtgctcgagg
     2221 tgcctcccgg tcccgcaggc cacgtcgagc agggtgtgcg cgccggggtt gcggtcctcg
     2281 acgatctcct gcaccctggc ggcctcggcc gggtagtcct tgaaggagta gatcgcgtcg
     2341 tagaagcgcg ccgtcttgtc gaacatgcgg ccatcgtagc ggcggaccgg cacaggcccg
     2401 gctcacctag aatcgagccg tgagcgaggt ccgcgaggtt ccgaccgacc agctcgcggc
     2461 ggtggtgcgc gaactgtgca tggagatctg ctaccgggtg ccgccggaga tggtggagct
     2521 gatgaagcgc gcccgggagc gcgaggagtc accgctcggc cggcagatcc tggaccagtt
     2581 gctgaagaac caggacatcg cggccgaggg ggagtacccg tactgccagg acaccggcta
     2641 cacggtggtg ttcctcgata tcggccagaa cgtgcacgtc accggcggtg acctgtacga
     2701 cgcgatcgac cgcggcgtgg ccgagggcta cacggacggc tacctgcgtg gctcgatcgt
     2761 ccgcgaccca ctgttcgacc ggaagaacac cggcgacaac accccggcct tcgtccacac
     2821 ccgcatcgtc ccgggcgacc aggtccggat ccagatcgac gccaagggcg ccggcagcga
     2881 gaacatgggg aggcacgcca tgctgaagcc cgccgacggc ctggagggcg tgaagcactt
     2941 cgtgctgaag gcggtggccg agtcgggacc gaacgcctgc ccgccgggga tcatcggggt
     3001 gggggtgggc gggaacttcg agatgagcgc ggtgctcgcc aagcgcgccc tgatgcgcaa
     3061 ggtcggccag ccgagccccg accccctgta cgccgagctg gaggccgagc tctacgagaa
     3121 gtgcaacgcg cttggcctgg gccccgaggc cctcgggggc acccagaccg tcctcgccgt
     3181 ccacgtcgag gggatgccca cccacatcgc cagcctcccc gtcgccgtga acatcgagtg
     3241 ccacgcccac cgcaccggcg tccgcacgat ctgatgccgg tcgagccgca gggcgacggg
     3301 cgggtgcgga tctcgccgcc gctcaccgac gacgacgtgc gttcgctcga ggcggggacg
     3361 gtcgtgctgg ttcggggggt ggtgtacggc gcgcgggacg cggcgcacaa gcggatgatc
     3421 gaggacctcg acgccgggcg ggacctgccg ttcgacccgg cggggcagat cgtctactac
     3481 gtcgggccca cgcccgagcg gcccgggaac gtgatcgggt cggccggccc gaccacggcg
     3541 tcgcggatgg acagatggac cccgacgcta ctggagcgcg gcctgaaggg gatcatcggc
     3601 aagggcggcc gcgggccggc ggtgcgccag gagctgaagc gacacaccgc cgtgtacatg
     3661 gccgcgcttg gcggcggggg ggcgctgtcg gcgctgacgg tccgttccca gcgcgtgatc
     3721 gcctacgagg acctcggcac cgaggcgatc cgcgcgatcg agatggacga cttcccggcc
     3781 tgggtggtga acgacgccgc cggacgcgac ttctacgccg agacgatccg gccctggcgc
     3841 aggaacgacc tgctgccgga tgacctgcac ctggaggagg ccgccgggaa ggcgatcgag
     3901 gagcgcggct tcctcggcgg gggctgagcc cgcccgcccc gcgtgagcgc cggcgtcccg
     3961 ttcgatcgcg ccgtcgcgtt ctacgaccgc acccggagcc tgccgccgga ggtgtcctcc
     4021 gcggtggcgc ggctcttggc gggtgagctc gaggggcggg gccgggccct cgaggggcgg
     4081 ggccgggccc tcgaggtggg ggcggggagc ggccggatca cgctgccgct cgcggcgagg
     4141 ggcatcccgc tggccgcggt cgatctctcg cggccgatgc tcgagcagct ccagcgcaac
     4201 gccggggggc gggcgccgtt cccggtggcc gtcgccgacg gcctggcgct gccgttcccg
     4261 tctggcgcct ttggcgccag cctgacctgc cacgtcctgc acctcgtcgc cggctggcag
     4321 gggttcctcg ccgaggtggt gcgcgtggtc cggccgggag gcgtggtcct gttcgacctg
     4381 ggcagcgagc ccccggagat ccaggagacg aaggagcggt tcgcccggga ggccggcctg
     4441 gagcgcgtct acgtcggcat gcgcgacccg gtggtgctcg accgggcgat ggccggcctc
     4501 ggcgcctcgg cccgcctgct cgagccgatc cggttcgaga cgcggcagcg gatccgggac
     4561 gtcctccgcg gctacgcggg cgcccagttc tcgttcacct gggtcgtcga ggacgaggcg
     4621 gtgctccggc gagccgctcg ggccacgcgc gcgtgggcgg cgggtcgctt cggtgacccc
     4681 gacgagcagc gaacgttcgt gcggacgatc cagtggcgtg cctacgacct gccgggagcg
     4741 gccggggcct gacccgatca gctccgtggc gaggcccggc gggccagcgc ctcgacgaga
     4801 gccgtggtcg tgccgttccc accgagatcc ggcgtcagcg cgtccccggc ggcgagggcc
     4861 tcctggaccg cccgctccac ccgcccggcc gcggcatgct ggccgaggtg tcgcagcatc
     4921 agcgcggccg acaggaccat ggcgatcggg ttggccacgc cctttccggc gatgtccggg
     4981 gcgctgccgt ggacgggctc gaacaccgcc gccgaatcgc cgatgttcgc gccggcggca
     5041 agccccgggc cgccgaccag gccggcgcac aggtcgctga cgatgtcacc gtagaggttt
     5101 ggcagcagca gcacgtcgag ctcctgcggc tgccaggcca gccacatggc cagctcgtcc
     5161 acctgcatct gctcgaaggc cacctcgggg tggaaggcgg cttcgccggc cgccgcgaac
     5221 aggaacaccc cgtcggagaa cttcatgatg ttcgccttgt ggccgacggt gatcttcgcc
     5281 cggccgtggc gcgcggcgaa gtcgaacgcg aagcgcacga tccggcgggt gccgccgatc
     5341 gagatgggct tgacggtcac gccggcgtcc tccgcgatgc ggtagccggc cagctcggcg
     5401 atctccgtgc gcagccgggc cagctcctcg gaccccttct cgaactcgat ccccgcgtag
     5461 aggtcctccg tgttctcgcg gatcacgacc aggtcgacgt tgtggtggcg ggtggccacc
     5521 cccgggagcg accgcgatgg ccggacggcg gcgaacaggt cgagctcctg gcgcagcgcc
     5581 acgttcacgc tgcggaagcc ggtcccgacc ggggtggtga ccgggccctt cagcgcggtg
     5641 ccccgctcgc ggatcgagtc cagcacgcgc gcggggaggg gcgtcccctc ggcctccacc
     5701 accgtggcgc cggcggcctg gacgtcccac tcgatctcgg ctccggccgc ctccaccacc
     5761 cgccgcgccg ccgcggccac ctcgggcccg atgccgtcgc cggggatcag cgtcacacga
     5821 tgcaccggtc catcatggac cggtgcatcg agccggcgga gccggctcca cggcatggag
     5881 agcgccgaag gcgctcctcg ccctcggtgg gcacacgggg ggcagagccc cccgtgctac
     5941 gtctcggtgg tgatgatcgt ccgcgtcccg ggaccgggga cgttgttcgg gaacaccacg
     6001 aggatcgacg ggtcctcgcc gggcgccgcc aagcctgccg agacgacgat cgggttcgcc
     6061 gcgttgcacg acgtcccgcc gaacgggcca cggcacacca ggtcgtcgat ctgcagggcc
     6121 gggtcgatga cgttcaggtg ggcccggtac gttccgcccg acatctgctc ggccttgtcg
     6181 cagatctggt cggggatgtc gcccgagtac cgcccggtgc aggcccgcac cccgtacgag
     6241 aacaccggat tcccatcgga caggccgatg tcgtccgcgt cgaacacgat gccggtgagg
     6301 ttgccgtcgt acagcgtgta ctcgttgaag tacgtcgcca ccgggttgcc gaacgggtcg
     6361 ggcaacgaca ggtcgtagac caccacgacg ccgccgggct cgggggtctt gaccgccagg
     6421 aagtctgcct tcaacccggg gtcggcgaac acgccgtcac cgcccacgtc gatcaggacg
     6481 tcgatctcgt tcgtgtcggt cgtgttgtgg atcccgttcg tctgcacggc gacctccacg
     6541 ggctcggccg ggaagttgtc gttcgtcagg aagatctgcc acggcagccc gaccagcggg
     6601 tcgaacccgt tcgggatccc ctccggcacc ccgtcgatcg agggcccggt gaaggaccgt
     6661 gcgccgatcg ccgagatgtc ctcctcaccg gtcgaggccg ggttcgggtc ggccgggtcg
     6721 agggccccga gcaggtacag gtccccgtag ttctggccgg ccccgccgtc cacgttcatg
     6781 gccagcgagc tctccgggag gacgcccgtc aggtcgaggt ggctcttgtt catcgagttg
     6841 ttcgacgccg cctcgggcac gatgtgccac ggaacgtgca gcgtgtccga cccgttcacg
     6901 ctctgctgga tcgtgacgtt cccgtccagg ccgtggttga agtagtacca gccgtacatg
     6961 tcgtcggcct tcgtgatcgc ggccgggtcc accgtcagac ggaccttgac ctgcttcgcc
     7021 gtcccggcgt tcagggtgaa ggacagcgtc gactgccagg tgccgccggt gacggcgaac
     7081 tgcagcgtgg ccagcggatt gctgaagtcc gagtaccgcg tgctcccggt gaccgtgtac
     7141 ttgtggggca cggaggccag gttctccacc gtgagcgtcc gctggaagct cgtggtggtc
     7201 ttcatcgact ggtacccgaa cgacaggctg cccggcgagg ccaggctctt cgccttcgcc
     7261 gactcgtagg cctgcacgcg tcccgacccc atcacggtcg ccgggaccgg ggtcgaggtg
     7321 tcgttgttct tcatcgcctg cgtggcctgg ttcatcaggg ccgccttgat ctgcagcggg
     7381 gtccacgtcg ggtggagctg ccgcagcagg gcggccacgc cggagacgtg cggggcggcc
     7441 atcgaggtcc ccgacagcag gatcttgtcg tcgccggtcc cgaccgccgc cgagccgatg
     7501 tcggagccgg gcgcggagat gtccggcttc aggtccgacg tcaggcgggc cggcccctcc
     7561 gaggtgaagt cggtcatcag gtcctcgaac cccgggatca cctcgggatt cgcgttgatc
     7621 gtcacgttca ccacgacggt gttgtacgag ccgggtgccc ccgggctggc ggcgtcggcc
     7681 agggccgcgc cgtccggccc gctgaccatg actgccggga tcgtcggcag cggcggcgcg
     7741 ctcggcccga ggccgaacgg ggcgccgccg aaccccgacc agaggatcac ggcgatggca
     7801 ccgtcgacct cggcgttgta gaccttggtg ctgccgccgc agtcgccggc gccggtggag
     7861 cccttgaaga tcagcgcgat cttcccggcc agcgggctgc cgcccaggct ggtgcagtac
     7921 tcctggtcgc tgggcacggg cgtgccgtcc ggcgcggcgg gcgggtcgag ctcgcggacg
     7981 tcgaagatgt cgccggtgat gtccgtggtg atgtcgcccg accagtcctg gtggacatag
     8041 atcccgccgt cggggaggac ggtcgccggg ctgttcacgt cgatcgtccc ggccgtgaac
     8101 tgatcgatcg aggcggccac ggcgaccacg ccgggggcga ccgccggcgt cccgacgatg
     8161 tagccgctgc cgccggaggg ctggttgccg gcgttgccgg cggaggcaac gaacaccgtc
     8221 ccgaggtcca ccacgccctg cgccgcgacg gcttccacgg agttcttggt gccgtagtcc
     8281 acgccgccgg agaacgacag cacgttcgcc cggtcgttcg tcgagccgtc ctggttgggg
     8341 tcgaccgcga actcgtagcc ggccaccagc acgtcgtccg acgagttgcc gacgtcccac
     8401 accttgaccg cgtagagctt cgccttcgga gctacgccgg gcccgacctt gccggggacg
     8461 ccgttgccgc agcacgtgcc gccggtgtgg gtgccgtgtc cgtcgacgtc gagcgggtcg
     8521 gggtcgggca ccgggatgtc gttggacggg tcgccgtcca ggacgtcgta gttgccgccg
     8581 acgaagtcgt agccgccgat caccttgccg gtcgggaagg tgccaggctc gatgatgtcg
     8641 gggtcgttgc cggcgtacgc gcccggagtc cccgggccgc cgaagtcctt gtgggtgtag
     8701 tcaatgccgg tgtcgaccag ggcgacccgc atcccctcgc cgcgcacgcc gaggttgttc
     8761 cagaccaggg gcgcgccgat gaacggcacg ctggtttcgt tggccctggt gacgacgccc
     8821 accggctgca ccgaggcgac gtcggtccgt cccgcgagcg ccgcggcggc ctgggccgac
     8881 atcttcgccg cgaacgcgtt cacggtccgc ccgtag
//
LOCUS       DASPWO010000446         7851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_744134, whole genome shotgun sequence.
ACCESSION   DASPWO010000446 DASPWO010000000
VERSION     DASPWO010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744134"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VEO00_10155"
     CDS             <1..768
                     /locus_tag="VEO00_10155"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYU58396.1"
                     /translation="FHSWTEMGQGVHTVLQQIACEELELPAERVHVVVDTSHELDTGE
                     TTASRATVLGGQAVIAAAKEVKAALNGGRLEDLAGKEFYGEFKVDWTTKNIEGLEEPV
                     THFAYGWGTQVVILDDEGRLARVVTANDAGRVINPTLLEGQVEGGIHMGLGYALTEEL
                     VVEGGVPVTDTLKSLGIIPPAGMPPVECIFVEEPQPEGPYGAKGVGEAPMVPTAAAVA
                     AALYAFDGVRRTRLPMRDSPAARAALPRLARTGGSDG"
     gene            765..2090
                     /locus_tag="VEO00_10160"
     CDS             765..2090
                     /locus_tag="VEO00_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYU58397.1"
                     /translation="MSLLLSGGMVASSLDPPRVAPADVAVEGGRIAPVPAPGAARIDC
                     AGCLVVPGNACGHTHLYSALARGMPFALEPPATFLQILQRVWWRLDRALDEESIRASA
                     LLGAMEALLAGTTSLVDHHASPNAVDGSLDVLAGALEEVGIRSVLCYEVTDRDGGERA
                     RAGVAENDRFLASLAARPRPLVRGMVGAHASFTLSEDTLAACVDVARRHDTGLHVHVA
                     EDGADERDARARFGTTVAARLARAGALDDRALLAHCVHLDGDEIALVRDSAAAVAHNP
                     RSNMHNGVGRAPVAALGPRVVLGTDGIGADMFEESRAGYFRLREDDLGAPAGWPLEWL
                     GETQRMVGRGYAEPLLGRLEPGAPADLVVLDYDPPTPIAAENLAGHWTFGLSAGAVRD
                     VIVGGEVVVRDRRPVRVDGAKVAAEAHAAAERLWARLERIDPHPFAPED"
     gene            2093..3112
                     /locus_tag="VEO00_10165"
     CDS             2093..3112
                     /locus_tag="VEO00_10165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU58398.1"
                     /translation="MAALPRVALYLQDKHPIRDGMAYARLAEEHGFEAVWQAESRLVR
                     EATVPMAAFAAVTERIKVGSGVVNTWTRNVGLLAATFSTLDDLAPGRILMGIGAWWEP
                     LASKVGVRRVRPLRAMREVVEVTRRLLAMERVTFHGEFVDVDDIEIDIVHGDRSPKRV
                     PIYVGATGMKMMELAGEIGDGALLNYLVGPSYNHRAMEALAEGAARAGRPVEALDRPQ
                     LVVCSLDQDRDAALDRARELVTQYLGQQPHIMKASGVDQALLDEIAAVLTWPAGPEEI
                     RRAMKLVPDEVVQLITASGTADECRAKVREYVEAGCTCPVLYPLGDDVEAMIGAFGTS
                     DGSAA"
     gene            3112..4002
                     /locus_tag="VEO00_10170"
     CDS             3112..4002
                     /locus_tag="VEO00_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HYU58399.1"
                     /translation="MDVLVPRTLVEALAMKAERPEAVPIAGGTDVMVEMNFGRLRPPA
                     MLDVSMLAELHELRRDDGQVVLGAGVPYARIIRELADHAPLVQASRTVGSPPIRNRGT
                     VGGNLGTASPAGDALPVLAVYDAEVVIARAGSEPRALAWDAFLTGPKANALAPDELIL
                     AARWRVPRGPGSFSKIGVRNAMVIAVASLTLQVDEDLRAVRVALGSVGPTILRAPEAE
                     AFAADALERSGAWDDPQAEIDEATLEGFADRVAAAARPIDDVRGAADYRRHGCAVLAR
                     RALSWALAERRPAAGSARVR"
     gene            4096..4656
                     /locus_tag="VEO00_10175"
     CDS             4096..4656
                     /locus_tag="VEO00_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58400.1"
                     /translation="MRIVTLTAVLVLGSSCGQRGQPDSPPPGGGNGPGIDWSDALNGN
                     GVEVPSLDAAQSQLTFTIVPPGKALGDPSSIQVSDPKQFAPADRALGLVYQGGAFGFF
                     VVIEGRTEYTQEFLESLPASCDESDACEGESLIKLADGSTALLLSGAPVTSVTWLDDG
                     IEIEVKGPAGSLTDQEAIGASNAFVS"
     gene            4795..5265
                     /locus_tag="VEO00_10180"
     CDS             4795..5265
                     /locus_tag="VEO00_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYU58401.1"
                     /translation="MLIRAQVNGEWVEAEVRPEASLLDLLRDGYLLMGTKNACTQGEC
                     GSCAVWLDGEVVCSCLVLAAQAEGREVRTVEGLADGDALHPVQAAFLRAGGVQCGFCT
                     PGLIVAATDLLQRDPDPREVDIREALAGNICRCTGYQKIVDAVRLAADALREAR"
     gene            5262..6638
                     /locus_tag="VEO00_10185"
     CDS             5262..6638
                     /locus_tag="VEO00_10185"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HYU58402.1"
                     /translation="MSEILISGCEAVATMDDAGTEIPGGSMLIRDGGIAWVGSGRPDG
                     AAGAEVVDGAGTVALPGFVNAHHHLFQALTRARAQRAGLFAWLRELYPVWAGLDEEWV
                     RAGAAVGLAELALSGCSTTTDHHYLFPAGAGDLLAAEIETAAEIGLRFQPGRGSMDLG
                     ESAGGLPPDAVVEDADAVLAATEDAVRRHHDPGPGAMVRVAVSPCSPFSVSDRLMRES
                     ADLARRLGVRLHTHIAETADEEAYCRERFGRRPVELLEDQGFLGPDVWLAHCVHLDDA
                     DVARLARTRTGVAWCPASNLRLGSGIARARDLLDAGAPVGFGVDGSASNDGGDLQAEA
                     RLAMLVARAGGDPGALDAREALRVATRGGAACLGRDDLGAIEPGRRADVALFDVGDLA
                     HAGAAADPVAAVLLCAPRGARDLFVEGRAVVRGGRLVTANQDAIAERGHRVARRILAR
                     TPAAKAAP"
     gene            6635..>7851
                     /locus_tag="VEO00_10190"
     CDS             6635..>7851
                     /locus_tag="VEO00_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYU58403.1"
                     /translation="MTGSAVLTGERTIAGVGASAARVDGVPKVKGAFVFGSDLHAEGM
                     LWGATLRSPHAHARIRSIDTAAAAAFPGVAAVLTAADLPAKRTFGLEFSDQPVLAADV
                     VRHEGEPVAIVAAQDTEVARDALARIVVDYEELPPLSDVVAALRPDAPAVHEHGNVLR
                     HVHIEHGDPDAPAEVWVEGYYETGMQDNAPLGPEAGLAFPAEDGGVDLFVTTQWLHVD
                     RQQIAPCLGLTEEKVRLYLAGVGGAFGAREDVHMQIHACMLALYTGRPVKMSYDREES
                     FYGHVHRHPSRVWMRHGARRDGRLVNIRARLLVDGGAYASSSSAVIGNASTFACGPYE
                     VPNALIEGTCVYTNNPPCGAMRGFGAVQAGFAYEAQMDKLAEALGIEPVELRLMNALH
                     TGSVLPTGQVILGA"
BASE COUNT         1014 a         2759 c         3011 g         1067 t
ORIGIN      
        1 ttccactcct ggaccgagat gggacagggc gtccacacgg tgctgcagca gatcgcctgc
       61 gaggagctcg agctcccggc cgagcgcgtg cacgtcgtgg tggacacttc gcacgagctc
      121 gacaccggcg agaccacggc ctcgcgagcg accgtcctcg gcgggcaggc cgtcatcgcc
      181 gcggcgaaag aggtgaaggc ggcgctgaac ggcggccgcc tcgaggacct ggccggcaag
      241 gagttctacg gcgagttcaa ggtggactgg acgacgaaga acatcgaggg cctggaggag
      301 ccggtgaccc acttcgccta cgggtggggc acgcaggtgg tgatcctcga cgacgagggc
      361 cggctggcaa gggtcgtcac cgcgaacgac gccggacgcg tgatcaaccc gacgctcctg
      421 gagggccagg tcgagggcgg gatccacatg ggcctgggtt acgcgctgac cgaggagctg
      481 gtggtcgagg gcggggtccc ggtcaccgac accctgaagt cgctcgggat catcccgccg
      541 gccgggatgc cgccggtgga gtgcatcttc gtcgaggagc cccagcccga ggggccgtac
      601 ggggcgaagg gagtgggcga ggcgccgatg gtcccgacgg cggccgccgt ggccgccgcc
      661 ctgtacgcgt tcgacggcgt ccgccgcacg cgcctgccga tgcgcgactc cccggccgcc
      721 cgcgccgcgc tcccgcgcct ggcgcggacg ggcgggagcg acgggtgagc ctcctcctct
      781 ccggaggcat ggtcgcctcc tccctcgacc cgccgcgcgt ggcgccagcg gatgtggctg
      841 tcgagggcgg gcgcatcgcc ccggtccccg cgccgggcgc cgcgcgaatc gactgcgccg
      901 ggtgcctggt cgtcccgggg aacgcctgcg gccacacgca cctctactcg gccctcgccc
      961 gcgggatgcc gttcgccctc gagccgccgg cgaccttcct gcagatcctg cagcgggtct
     1021 ggtggcgcct cgatcgcgcc ctggacgagg agtcgatccg cgcgtcggca ttgctcggcg
     1081 cgatggaggc cctgctggcc ggcaccacgt cgctggtcga tcaccacgcc tccccgaacg
     1141 ccgtggacgg gtcgctcgac gtcttggccg gggccctcga ggaggtcggg atccgctcgg
     1201 tgctctgcta cgaggtcacc gaccgcgacg gcggcgagcg cgctcgggcc ggcgtggccg
     1261 agaacgatcg gttcctggcc tcgctggccg cccggccccg gccgctggtg cgggggatgg
     1321 tcggggcgca tgcttcgttc acgctgtccg aggacacgct cgccgcctgc gtcgacgtgg
     1381 cccgccgcca cgatacaggg ctccacgtgc acgtcgccga ggacggcgcc gacgagcggg
     1441 acgcccgggc gcggttcggc acgacggtcg cggctcgact ggcgcgcgcc ggggccctcg
     1501 acgaccgcgc gctcctggcg cactgcgtcc acctcgacgg cgacgagatc gcgctggttc
     1561 gggacagcgc cgcggcggtg gcgcacaacc cgcgctcgaa catgcacaac ggagtcggcc
     1621 gggcaccggt ggccgcgctc ggcccgcgcg tggtgctcgg cacggacggc atcggcgccg
     1681 acatgttcga ggagtcccgc gccggctact tccggctgcg cgaggacgac ctcggcgccc
     1741 ccgcgggctg gccgctggag tggctgggcg agacgcagcg gatggtcggc cgggggtacg
     1801 cggaaccgct cctcgggcgt ctcgagcccg gcgcgcccgc cgacctcgtc gtgctcgact
     1861 acgacccgcc gacgccgatc gccgcggaga acctggccgg ccactggacc ttcgggctgt
     1921 ccgcgggcgc ggtgcgcgac gtgatcgtcg gcggagaggt cgtggtccgt gaccgccggc
     1981 cggtgcgcgt cgacggggcg aaggtcgccg ccgaggccca cgccgccgcc gagcgactgt
     2041 gggcccgcct ggagaggatc gacccccacc cgttcgcacc ggaggactga aggtggcggc
     2101 gctcccccga gtcgcgcttt acctgcagga caagcacccg atccgcgatg ggatggcgta
     2161 cgcgcggctc gccgaggagc acgggttcga ggccgtctgg caggcggaga gccgcttggt
     2221 gcgcgaggcg acggtcccga tggccgcctt cgccgcggtg accgagcgga tcaaggtcgg
     2281 ctccggcgtc gtcaacacgt ggacgcggaa cgtcggcctc ctggcggcga cgttctcgac
     2341 gctcgacgat ctggcgcccg ggcggatcct gatggggatc ggggcgtggt gggagccgct
     2401 cgcgtcgaag gtcggcgtgc gccgggtgcg cccgctgcgg gcgatgcgcg aggtggtcga
     2461 ggtgacgcgg cggctgctgg cgatggagcg ggtcaccttc cacggggagt tcgtggacgt
     2521 ggacgacatc gagatcgaca tcgtccacgg cgaccggtcg ccgaagcggg tcccgatcta
     2581 cgtcggcgcc acggggatga agatgatgga gctcgccggg gagatcgggg acggcgcgtt
     2641 gctgaactac ctggtcggcc cgtcgtacaa ccaccgggcg atggaagccc tggccgaggg
     2701 ggcggcgcgg gccgggcgac ccgtcgaggc gctggaccgt ccgcagctgg tggtgtgctc
     2761 gctcgaccag gaccgcgacg ccgcgctcga tcgggcgcgc gagctggtca cccagtacct
     2821 gggccagcag ccgcacatca tgaaggcgag cggcgtggac caggcactgc tggacgagat
     2881 cgccgcggtc ctcacatggc cggcgggccc ggaggagatc cgccgggcca tgaagctcgt
     2941 ccccgacgag gtggtccagc tgattacggc ctcgggcacc gccgacgagt gccgggccaa
     3001 ggtccgcgag tacgtcgagg ccggctgcac gtgccccgtg ctctacccgc tcggcgacga
     3061 cgtggaggcg atgatcggtg ccttcggcac gagcgatgga tcggcggcgt gatggatgtc
     3121 ctcgtgcccc ggacgctggt cgaggcgctg gcgatgaagg cggaacgtcc cgaggccgtc
     3181 ccgatcgccg gcggcacgga cgtgatggtg gagatgaact tcgggcgcct gcgtccgccg
     3241 gcgatgctcg acgtgtcgat gctggcggag ctccacgagc tgcgacgtga cgacggccag
     3301 gtcgtcctcg gagcgggcgt gccctacgcg cggatcatcc gcgagctggc cgaccacgcg
     3361 ccgctcgtcc aggcctcgcg cacggtcggg tcgccgccga tccgcaaccg cggaacggtc
     3421 ggcggcaacc tggggacggc gtcgccggcc ggcgacgcgc tccccgtcct ggcggtctac
     3481 gacgccgagg tcgtcatcgc gcgcgccgga agcgagccgc gcgcgctggc ctgggacgcc
     3541 ttcctgaccg gcccgaaggc gaacgccctc gcccccgacg agctgatcct cgccgcgcgc
     3601 tggcgcgttc cgcgggggcc ggggtcgttc tcgaagatcg gcgtgcgcaa cgcgatggtc
     3661 atcgcggtgg cgagcctgac gctgcaggtg gacgaggatc tccgcgcggt gcgcgtggcg
     3721 ctcggctccg tcggcccgac gatcctgcgt gccccggagg cggaggcgtt cgccgccgac
     3781 gcgctcgaac ggtccggcgc ctgggacgac ccgcaggcgg agatcgatga ggcgacgctg
     3841 gagggcttcg ccgaccgcgt cgccgccgcc gcccgcccga tcgacgacgt ccgcggcgcc
     3901 gccgactacc gccgacacgg ctgtgccgtc ctcgcccgcc gcgccctctc ctgggctctg
     3961 gccgagcgcc gcccggcggc gggatcagca cgagtccgct gaagcgaaac ggcggtcaag
     4021 tccttcgatg cgtcagacag aaccgccacc cctcctcggg cgttgacgat gcaacgatcc
     4081 gggatgtcag cccgcgtgag gatcgtgacc ctgacggccg tgcttgtact cgggtcctcc
     4141 tgtgggcagc gcggacagcc ggacagccca cctccgggtg gtggcaacgg tccggggatc
     4201 gactggtcag atgccctgaa cgggaacggc gtggaggtac cgtcgctcga cgcagcccag
     4261 tcgcagctga cgttcacgat cgtgcccccc gggaaagccc tcggggaccc gagcagcatc
     4321 caggtcagcg atcccaagca gttcgcgccc gcggatcggg cactgggcct cgtgtaccaa
     4381 ggtggagcgt tcgggttctt cgtggtgatc gagggccgaa cggagtacac gcaggagttc
     4441 ctcgagtcgc tcccggcttc gtgcgatgag agcgatgcct gcgaaggtga gtctctcatc
     4501 aagctcgcgg acggttccac cgccttgctt ctgtccggtg ccccggtcac ctcagtgacc
     4561 tggctggacg acggcattga gatcgaggtc aagggacccg cggggtccct cacggaccag
     4621 gaggcgatcg gcgcctcgaa cgccttcgtc agctgaacct ccgcgggtcc cgtctcgccc
     4681 ggttgggaac gacccctcgt tcggattaga ttcggctgtc gcgcggcatt gagagcctgg
     4741 gtcgatccgg ggggaacgcg ggctctcgcg gacaatggcc gtggaggtgc cgaggtgctg
     4801 atccgggcgc aggtcaacgg ggagtgggtg gaggccgagg ttcggcccga ggcgagcctg
     4861 ctcgacctgc tccgggacgg gtacctgctg atggggacga agaacgcctg cacccagggc
     4921 gagtgcgggt cgtgcgcggt gtggctggat ggcgaggtcg tctgctcgtg cctggtgctc
     4981 gccgcccagg ccgaggggcg ggaagtgcgc acggtcgagg gtctcgcgga cggcgacgcg
     5041 ctgcaccccg tgcaggcggc gttcctgcgg gccggtggcg tgcagtgcgg gttctgcacg
     5101 ccggggctga tcgtcgccgc caccgacctc ctccagcgcg acccggatcc ccgcgaggtc
     5161 gacatccgag aggcgctcgc cggcaacatc tgccgctgca ccggctacca gaagatcgtc
     5221 gacgcggtcc gcctggccgc cgacgcgctc cgcgaggctc gatgagcgag atcctgatct
     5281 ccggctgcga ggccgtggcg acgatggacg atgccggcac cgagatcccg ggcgggtcga
     5341 tgctgatccg ggacgggggg atcgcctggg tggggagcgg ccggccggac ggggccgcgg
     5401 gcgcggaggt cgtcgacggc gccggtacgg tggcgctccc ggggttcgtc aatgcccacc
     5461 atcacctctt ccaggcgctg acgcgggcgc gggcgcagcg cgccggcctg ttcgcctggc
     5521 tgcgagagct gtacccggtg tgggcaggtc tggacgagga gtgggtccgg gcgggggcgg
     5581 ccgtcgggtt ggccgagctg gccctctccg ggtgctcgac gacgacggac caccactacc
     5641 tgttccccgc cggagccggc gatctgctgg cggccgagat cgagacggcg gcggagatcg
     5701 gcctgcgctt tcagcccggc cgcggctcga tggacctcgg ggagtccgcc ggcgggctgc
     5761 cacccgacgc ggtcgtcgag gacgccgacg cggtgctggc ggcgaccgag gacgccgtgc
     5821 gccggcacca cgaccccggc ccgggggcga tggtccgcgt cgccgtctcg ccgtgctccc
     5881 cgttctcggt cagcgaccgc ctgatgcggg agtcggccga tcttgcccgg aggctcggcg
     5941 tgcgactgca cacgcacatc gccgagaccg ccgacgagga ggcctactgc cgggagcggt
     6001 tcgggcgccg gccggtggag ctgctcgagg accagggctt cctcggtccg gacgtctggc
     6061 tggcgcactg cgtccacctc gacgacgccg acgtggcgcg cctggcgcgc acccggaccg
     6121 gcgtggcgtg gtgcccagcc tccaacctgc ggctcggctc ggggatcgcc cgggcgcgcg
     6181 acctgctgga cgccggcgcg ccggtcgggt tcggcgtcga tggctcggcc tcgaacgacg
     6241 gcggggacct gcaggccgag gcccgcctgg cgatgctcgt cgcgcgcgcc ggcggtgatc
     6301 ccggagcgct cgacgcccgc gaggcgctgc gggtcgccac ccgcggcggg gcggcctgcc
     6361 tcgggcgcga cgacctcggg gcgatcgagc cgggccgccg cgccgacgtg gccctgttcg
     6421 acgtcggcga tcttgcgcac gccggggcgg ccgccgatcc ggtggccgcg gtcctgctct
     6481 gtgcgccccg cggcgcgcgc gacctgttcg tcgagggccg ggccgttgtg cgcggcgggc
     6541 gcctcgtcac cgccaaccag gacgcgatcg ccgagcgcgg ccaccgggtc gcccggcgga
     6601 tcctggcgcg aacgccggcg gcgaaggccg cgccgtgacc ggctccgccg tcctgaccgg
     6661 cgagcggacc atcgccggcg tcggcgcctc ggccgcgcgc gtcgacggcg tcccgaaggt
     6721 gaagggggcg ttcgtcttcg gcagcgacct ccacgccgag ggcatgctgt ggggggcgac
     6781 gctgcgcagt ccccacgccc acgctcgcat ccggtccatc gacaccgccg ccgcggcggc
     6841 gttccccggc gtcgcggccg tgctcaccgc cgccgacctc ccggccaagc ggacgttcgg
     6901 gctggagttc tccgaccagc cggtgctggc cgccgacgtc gtgcgccacg agggcgagcc
     6961 ggtggcgatc gtggccgccc aggacacgga ggtcgcccgg gacgcgctgg cgcggatcgt
     7021 cgtggactac gaggagctgc cgccgctgag cgacgtcgtg gcggcgctgc gccccgacgc
     7081 gccggccgtg cacgagcacg gcaacgtcct gcgccacgtc cacatcgagc acggcgaccc
     7141 cgatgcgccg gccgaggtgt gggtcgaggg ctactacgag accgggatgc aggacaacgc
     7201 cccgctcggg cccgaggccg ggctggcgtt cccggcggag gacggcgggg tcgacctgtt
     7261 cgtcacgacg caatggctgc acgtcgaccg ccagcagatc gcgccctgcc tggggctgac
     7321 cgaggagaag gttcgcctgt acctggccgg cgtgggcgga gcattcggcg cccgggagga
     7381 cgtccacatg cagatccacg cgtgcatgct cgccctgtac accggccgcc cggtgaagat
     7441 gtcctatgac cgtgaggagt cgttctacgg ccacgtgcac cgtcacccct cacgggtgtg
     7501 gatgcgtcac ggggcgaggc gggacggacg gctggtgaac atacgggcgc ggctcctcgt
     7561 cgacggcggg gcctatgcct cgagctcttc ggcggtgatc ggcaacgcct cgacgttcgc
     7621 gtgcggaccg tacgaggtgc ccaacgcgct gatcgagggg acctgcgtgt acaccaacaa
     7681 cccaccctgc ggggccatgc gaggctttgg cgccgttcag gcggggttcg cctacgaggc
     7741 ccagatggac aagctggccg aagcgctcgg catcgagccg gtcgagcttc gcctgatgaa
     7801 cgccctgcac acgggctcgg tgctccccac cgggcaggtc attctgggcg c
//
LOCUS       DASPWO010000447         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_744322, whole genome shotgun sequence.
ACCESSION   DASPWO010000447 DASPWO010000000
VERSION     DASPWO010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744322"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..574)
                     /locus_tag="VEO00_10195"
     CDS             complement(146..574)
                     /locus_tag="VEO00_10195"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HYU58404.1"
                     /translation="MSEAVELARKIVDLTDSGNFRAREHLIHPDCEIVMSGAGRLTRQ
                     EFTRYSQALATAFPQAEHVFEQTLEFAGTVVIEGMWIATQTGPFETPQGRIPATGRTM
                     RTRLCLVFYILDGLAVSIHGYWDQLEMLQQLGLVPEPAAL"
     gene            complement(671..>2199)
                     /locus_tag="VEO00_10200"
     CDS             complement(671..>2199)
                     /locus_tag="VEO00_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYU58405.1"
                     /translation="AEAMAVMSLATDVSMGQPMEESLAVCLLATRLADELGLSDADRV
                     HVYYTALLRHIGCTADMHVLAAVAGDDISMRNAFVGVDLGRPVEMFAAMMRHIGRTYA
                     PLERPAAVVRVMAATGKFRHGGEVQCEVAEMLAGRLGFDESFQRDISSVLERWDGKGF
                     PGKVGGEEITMAARVVGVAELAALSHRMDGPEAALDVLRRRSGKAFDPAVVEGFVSNG
                     TKLFAALETASPWDGVLEVEPGERPMLSGRRLDEALRAMGDFADLKSPYTVGHSSGVA
                     DLAARAAERCGLPPGDVIAARRAGWVHDIGRVAVSSAIWDKPGPLTRGEWEKVRLHPY
                     HADRVLARQGFLDSLGRIASMHHERLDGSGYFRQAPAGQQPPIVRVLAVADAYHAMTE
                     PRPHRPAMTPEQAAAELRAEIKAGRLDAEAVRGVLDAAGMPVGRRAEQVAGLTVREVQ
                     VLRLLARGQSIRDIARTLQIAPKTADAHIQHIYTKAGVSSRAAATLFAMQHDLVAPHA
                     "
BASE COUNT          308 a          788 c          779 g          324 t
ORIGIN      
        1 ttcaaggcgc tgttcgaggc gatcgagcgc gagcaggcgc gccgcgggaa cctctgagca
       61 gcccgctgcc cgctgacgag atggggccgg tccttccggg ccggccccat cgtccgcccc
      121 cccgggaccc tcattcccgg cgaggtcaga gcgccgccgg ctcaggcacg agcccgagtt
      181 gctgcaacat ctcgagttgg tcccagtagc cgtggatcga cacggccaga ccgtcgagga
      241 tgtagaagac cagacacagc cgcgtgcgca tcgtccgtcc ggtggccggg atgcggccct
      301 gcggcgtctc gaacggtcct gtctgcgtgg cgatccacat gccctcgatc accaccgtgc
      361 ccgcgaactc cagggtctgc tcgaagacgt gctccgcctg cgggaacgcc gtggcgagcg
      421 cctggctgta gcgggtgaac tcctggcggg tcagccggcc ggcgccggac atgacgatct
      481 cgcagtccgg atggatcaag tgctcgcggg ctcggaagtt cccgctgtcg gtgaggtcga
      541 cgatctttct cgccagctcc accgcttccg acatccgcac cgtctcctca ggtcaacgtg
      601 caaccggagg atgatccctg gcgtggtcgg ctccatcggg agttttccca atctttgccg
      661 ggctcaggcc ctatgcgtgg ggcgcgacca gatcgtgctg catggcgaag agcgtggccg
      721 cggcgcggct ggagacgccg gccttggtgt agatgtgctg gatgtgggcg tcggcggtct
      781 tcggggcgat ctgcagtgtg cgggcgatgt ccctgatcga ctggccgcgc gccagcagcc
      841 ggagcacctg gacctcgcgg acagtgagcc cggcgacctg ctcggccctg cggccgaccg
      901 gcatcccagc cgcgtcgagc accccgcgca cggcttccgc gtcgagcctg ccggccttga
      961 tctcggcgcg cagctcggcg gccgcctgct cgggggtcat ggccggccgg tgcggccgcg
     1021 gttcggtcat cgcgtggtag gcgtcggcga cggcgaggac cctgacgatc ggcggctgct
     1081 gtcccgccgg cgcctgccgg aagtagccgg agccgtccag gcgctcgtgg tgcatcgagg
     1141 cgatgcgccc gagggaatcg aggaacccct ggcgcgccag gacccggtcg gcgtggtagg
     1201 ggtgcaggcg gaccttctcc cactcgcccc gggtgagcgg gccgggtttg tcccagatcg
     1261 ccgaggacac ggctacccgg ccgatgtcgt gcacccaacc cgccctgcgg gcggcgatga
     1321 cgtcgccggg gggaaggccg cagcgttccg cggcgcgggc ggcgaggtcg gcgacgccgg
     1381 aggagtgacc gacggtgtac ggggacttga gatccgcgaa gtcccccatg gcacgcaggg
     1441 cctcgtccag tcgtctcccc gacagcatcg ggcgctcgcc cggctcgacc tcgagcacgc
     1501 cgtcccaagg ggaggcggtc tcgagcgctg cgaacagctt ggtcccgttg gacacgaacc
     1561 cttcgacgac ggcggggtcg aacgccttgc cggaacggcg ccgcaggacg tcgagcgcgg
     1621 cctcgggccc gtccatgcga tggctgaggg ccgcgagctc ggccacgccg accacccggg
     1681 cggccatcgt gatctcctcg ccgccgacct tgccggggaa ccccttgccg tcccagcgct
     1741 ccaggacgct cgagatgtcg cgctggaagc tctcgtcgaa cccgaggcga cccgcgagca
     1801 tttccgcgac ctcgcactgg acctcgcccc cgtgccggaa cttcccggtc gcggccatga
     1861 cgcgcaccac ggccgccgga cgttccagcg gcgcgtaggt ccgaccgatg tgccgcatca
     1921 tcgcggcgaa catctcgacc ggccggccga ggtccacccc gacgaacgcg ttccgcatcg
     1981 agatgtcgtc gccagccacc gccgccagca cgtgcatgtc ggcggtgcag ccgatgtggc
     2041 ggagcagggc ggtgtagtac acgtgcacgc gatcggcatc ggacagcccc agctcgtccg
     2101 ccagccgcgt ggccaggagg cagaccgcca ggctctcctc catcggctgg cccatgctga
     2161 cgtcggtggc gagcgacatc acggccatcg cctcggcca
//
LOCUS       DASPWO010000448         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1249200, whole genome shotgun sequence.
ACCESSION   DASPWO010000448 DASPWO010000000
VERSION     DASPWO010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249200"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VEO00_10205"
     CDS             <1..495
                     /locus_tag="VEO00_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HYU58406.1"
                     /translation="EHVDTPMDFESIQAAGSLLGTGAMIVLDDRDCIVEATLRFTQFY
                     AHESCGKCTPCREGTWWMTRVLQRMERGDGRSEDIDLIKDVGSNMLFKSFCALADGAV
                     SPLDSSIKYFRDEYEEHVRLGRCPLKEEPVMAAATDRSGGTTGGTAQVLPEGREINLG
                     EVLG"
     gene            492..>2311
                     /gene="nuoG"
                     /locus_tag="VEO00_10210"
     CDS             492..>2311
                     /gene="nuoG"
                     /locus_tag="VEO00_10210"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HYU58407.1"
                     /translation="MTNGAGGGPVTLAIDGKEVTVPQGTLIIRAAEQLGIEIPRFCDH
                     PLLEPVAACRQCYVKVEGQRKLMTSCSTPVAQDMAVHTQFTDDEVREAQEAVLEFLLI
                     NHPLDCPICDRGGECPLQDQALAYGPGESRYVEAKRTYRKPLPLSPLVNLDRERCVLC
                     ARCTRFCDQISGDRFIELFDRGAAEQVSIAPGEDFRSPFSGNTIQICPVGALTATTYR
                     FAARPFDIKSGDTICPHCACGCNLKVDLRRGEVVRHLARDNAEVNEAWLCDKGRFAFS
                     FPDRPTRLTTPLLRERGLEPVSFGEALEAVARWSKDDGSVAFLAGGRLSDEDAYALSK
                     LARAVFRTNDVDHRLTSPSDVPLEVDRAHAAGMPVTYRDVERAKAILVVGLDAEQELP
                     ILHLRIRKAARRGAKVFVLHPRRTRLWDLTEHLPCAPGEEAAVLEGLESAEDGSVESR
                     VLAALRDAGEAGVVLAGPRLADSPGGVGAAAALAGEFGAKFAHLSRRANDRGALRAGL
                     HTGLLPGGRSVEDTAARARVRTEWDMPVPAEPGRDTAAILDAAADREIGVLFLVGVDP
                     LRDYPDARLARRALENVPYKVVVDVSAETMSSYADVMLPAA"
BASE COUNT          338 a          790 c          846 g          337 t
ORIGIN      
        1 gaacacgtcg acacgcccat ggacttcgag tcgatccagg ccgccggttc gctcctgggg
       61 accggcgcga tgatcgtgct ggacgaccgc gactgcatcg tcgaggccac cctgaggttc
      121 acccagttct acgcccacga gtcctgcgga aagtgcacgc cgtgccgcga gggaacctgg
      181 tggatgacgc gggtgctcca gcggatggag cggggcgacg ggcggagcga ggacatcgac
      241 ctgatcaagg acgtcggcag caacatgctg ttcaagtcgt tctgcgcgct ggccgacggg
      301 gcggtctctc ccctcgactc ctcgatcaag tacttccgtg acgagtacga ggagcacgtc
      361 cggctgggcc ggtgcccgct gaaggaggag ccggtgatgg cggcggcgac ggaccgctct
      421 ggaggcacca ccgggggcac cgcccaggtc ctgccggagg gccgggagat caacctggga
      481 gaggtcctgg gatgaccaac ggggcgggcg gcgggccggt caccctggcc atcgacggca
      541 aggaggtgac cgttccccag gggacgctca tcatccgggc cgccgagcag ctggggatcg
      601 agatcccccg gttctgcgac cacccgctgc tcgagcccgt cgccgcctgc cggcagtgct
      661 acgtgaaggt cgagggccag cgcaagctca tgacctcgtg ctccacgccg gtggcccaag
      721 acatggcggt ccacacccag ttcaccgacg acgaggtgcg ggaggctcag gaggcggtcc
      781 tggagttcct cctgatcaac cacccgctcg actgcccgat ctgcgatcgg ggcggtgagt
      841 gtccgctcca ggaccaggcg ctcgcttacg ggccggggga aagccggtac gtcgaggcga
      901 agcgcacgta ccgaaagccg ctgccgctgt cgcccctggt gaacctggat cgcgaacggt
      961 gcgtgctgtg cgccagatgc acccggttct gcgatcagat ctccggcgac cggttcatcg
     1021 agctgttcga ccggggggcg gccgagcagg tctcgatcgc tcccggcgag gacttccgct
     1081 cgccgttctc cggcaacacg atccagatct gtccggtcgg agcgctgacc gcaacgacct
     1141 accggttcgc cgcccgcccg ttcgacatca aatcgggcga cacgatctgc ccccactgcg
     1201 cctgcgggtg caacctgaag gtcgacctgc ggcggggaga ggtcgttcgg cacctggccc
     1261 gggacaacgc cgaggtcaac gaagcttggc tgtgcgacaa gggccggttc gccttctcct
     1321 tccccgaccg gccgacccgg ctgaccaccc cgctcctgcg cgagcgcggc ctcgagcccg
     1381 tctcgttcgg cgaggcgctc gaggccgtgg ctagatggtc gaaggacgac gggtcggtgg
     1441 cgttcctggc cgggggacgg ctttcggacg aggacgccta cgccctgtcg aagctggctc
     1501 gcgcggtgtt ccggaccaac gacgtcgacc accgtctcac cagcccgtcc gacgtccccc
     1561 tcgaggtcga tcgggcccac gcggccggta tgccggtgac ctaccgggac gtggagcggg
     1621 ccaaggcgat cctggtggtc gggctggacg ccgagcagga gctgcccatc ctgcacctgc
     1681 ggatccggaa ggcggcccga cggggggcga aggtgttcgt cctgcatccg cgccggacgc
     1741 gcctgtggga cctcaccgag cacctgccgt gcgcgccggg agaggaggcc gccgtcctcg
     1801 aggggctcga gtcggcggag gacggctcgg tcgagagccg ggtcctggcg gccctgcgcg
     1861 acgcgggcga ggccggagta gtcctggctg gaccgcggct ggcggattcg ccgggtgggg
     1921 ttggcgccgc cgccgctctg gccggggagt tcggcgcgaa attcgcccac ctttcgcggc
     1981 gggccaacga ccggggggct ctccgtgccg gcctgcatac gggtctgctg ccgggcggca
     2041 ggtcggtcga ggacacggcg gcccgtgcca gggtgcgcac tgagtgggac atgccggttc
     2101 ccgccgaacc gggccgggac accgcggcga tcctcgatgc cgccgcggac cgggagatcg
     2161 gggtcctgtt cctggtgggc gtggaccccc tgcgcgacta ccccgacgcc cggctcgcca
     2221 ggcgggccct ggagaacgtg ccgtacaagg tggtggtcga cgtctcggcc gagaccatgt
     2281 ccagctacgc ggacgtgatg ctccccgccg c
//
LOCUS       DASPWO010000449         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_658404, whole genome shotgun sequence.
ACCESSION   DASPWO010000449 DASPWO010000000
VERSION     DASPWO010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658404"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..334
                     /locus_tag="VEO00_10215"
     CDS             53..334
                     /locus_tag="VEO00_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58408.1"
                     /translation="MPRKHRAARERGVPLEAPDRPRGVAPEWASAEGVRVQAVTGEKG
                     KTYRCPGCQQEIRPGTPHLVVMEHEDVGFRRHWHTPCWRSELRRRGLSA"
     gene            404..868
                     /locus_tag="VEO00_10220"
     CDS             404..868
                     /locus_tag="VEO00_10220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU58409.1"
                     /translation="MKVSVDEGVELLYREHGQRLWRAVMAFTGDREVASDAVAEAFAQ
                     ALRRGDVINDPLRWVWRAAFRIAAGELKERGRFREASTDRGYDQDEPVTDLLEALAKL
                     SPKQRAAVLLHHHAGYPVKEVADIIGSTTAAVKVHLMRGRNRLRDLLGEEHD"
     gene            1695..>2646
                     /locus_tag="VEO00_10225"
     CDS             1695..>2646
                     /locus_tag="VEO00_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58410.1"
                     /translation="MAATGGPSRKLTDCGAIPDCIDALDPTWSPDGRLIVFEVERDGG
                     RRSLFLMNADGTGLRQLTFHNDDCCASWQPLPSSPSTEHWSVAANGVIWARVGGGDGP
                     SFVYSVDPDGSGKTLLFSDGRDPNSPPETVNPEAVGEDYAWTPEGSQVAFLHSTNFDP
                     GRGQFSAIFAMNPDGSSLTQLTPDGEVDSGPAWSPDGARIAYASDRTGPFSEGAGCWA
                     TYLCSGDIHVMNADGSGDIQLTDDPADDSQPAWSPDGTRIAFVSQRDDANRGDIFVMS
                     ADGSGLTQLTSGRDWDRSPHWSPDGSVIAFLSYGDRAWNVY"
BASE COUNT          407 a          896 c          937 g          406 t
ORIGIN      
        1 ggcgggagct cggcgaggtc gccgccgcct tcatcaggga ggtggtggcg tagtgccccg
       61 gaagcaccgg gcggcccgcg agcggggagt cccgctggag gctccggacc ggccgcgggg
      121 cgtggccccc gagtgggcgt ccgcggaggg cgtgcgggtc caggcggtga ccggcgagaa
      181 gggcaagacc taccggtgcc ccggatgcca gcaggagatc aggccgggga ccccgcacct
      241 cgtggtgatg gagcacgagg acgtggggtt ccggcggcac tggcacacac cgtgctggcg
      301 aagtgagctt cgccgccgcg gcctgagcgc gtaacccgct tgaccccgct gttcacctgt
      361 tgggcccttt cggaccctgt atgcacgggt gcaggatgcg ctggtgaagg tgagcgtcga
      421 cgaaggcgtg gagctcctct accgcgagca cggtcagcgg ctgtggcgcg ccgtcatggc
      481 cttcaccggc gatcgggagg tggcgagcga cgccgtggcc gaagccttcg cccaggccct
      541 gcgaaggggc gacgtgatca acgacccgct gcggtgggtc tggcgagctg cctttcgcat
      601 cgctgccggc gagctcaagg aacgaggtcg gttccgcgag gcctccacgg accgcggtta
      661 cgaccaggac gagccggtca cggacctgct ggaggccctg gcgaagctct cgccgaagca
      721 gcgggccgcg gtgctgctgc accaccacgc cggttatccg gtgaaggagg tggccgacat
      781 catcggctcg accactgcgg cggtgaaggt ccatctgatg cgaggccgaa accgtcttcg
      841 cgacctactg ggagaggaac atgactgagc tggacgaacg cctgaagctg ctggatcgta
      901 tggagccgcc cgatctgtgg gcggacatcc gcagccgcga gccgcgccgg ccggcgtccc
      961 cggagggacg tgggcgggtg gtcgccgtct ttgcggctgc agtggtcgcg gcggggggga
     1021 tcgccctgct ggcgagggcc ttcctcggcg gaacgcccaa accgtcgagc caacccttcc
     1081 atccggcggc gaaggcgaac ggagtggtcg ccttcgtgcg aggcacccgt gggccgaact
     1141 ccggtatcta cgtcatccag cccgacggta ccggcgcccg ccggctggcg gcaggggcag
     1201 gcgaatacgg cgaggcgacg tggtctcctg acgggtccag gatcgccttc acccggtcgg
     1261 atggacccgg gtcctcatcg gttcagggac tcttcgccgc ggccgccgac ggttcacatg
     1321 cgcgccggct gttccaatgc ggagcccggg ggtgccgaga gcccgcgtgg tcgccggacg
     1381 gccgccggat cgccttcgta tggggtcagg acatctatac ggtgaacgcc gaggggactg
     1441 acatccgtcg cctcacccga gcccggaccc cgctcggcga cggccaccct gcatggtcgc
     1501 ctgacggaac ccggatcgcg ttcatcgtcc tgcgggtcca gccttacgac ctcccgggca
     1561 tcttcgtgat gaacgcggac gggagccacg tccggcggct cacgaactgt ctgccgggtt
     1621 gtgtccagag cctgcccgcg tggtcaccgg acggccgcac gatcgcgttc tccgggcaga
     1681 tcgacgtctt cgtgatggcc gcaaccggcg gaccaagccg aaagctgacc gattgcggcg
     1741 cgatcccgga ctgcatcgac gctctggatc cgacctggtc tcccgatggg cggctgatcg
     1801 tgttcgaggt cgagcgcgac ggcggccgcc ggtcgctctt cctgatgaac gccgacggca
     1861 ccggcctcag gcagctcacg ttccacaacg atgactgctg tgcgtcctgg cagcccctcc
     1921 cttcatcccc ttcgaccgag cactggtccg tcgctgcgaa tggcgtgatc tgggcccgtg
     1981 tgggcggagg cgacggaccg agcttcgtgt acagcgtcga ccccgacggt tccggcaaga
     2041 ctctgctgtt cagcgacggg cgcgatccga actctcctcc ggagaccgtc aacccagagg
     2101 ccgttggcga ggactacgcg tggacacccg aagggtccca ggtggccttc ctgcactcga
     2161 ccaacttcga cccgggccga ggtcagttca gcgcgatctt cgccatgaac ccggatgggt
     2221 cgagcctgac ccagctcacg cccgacggcg aggtcgacag cgggccggcc tggtccccag
     2281 atggcgccag gatcgcctac gccagcgacc gaaccggtcc gttctccgag ggcgccggat
     2341 gctgggccac ctacctatgc tcgggcgaca tccatgtgat gaacgccgac ggctcgggcg
     2401 acatccagct cacggacgac ccggccgacg actcgcagcc cgcctggtca ccagacggca
     2461 cgagaatcgc cttcgtcagt cagcgcgacg acgcgaaccg cggggatatc ttcgtgatga
     2521 gcgcggatgg aagcgggctc acccagctta cgtccggtcg tgactgggac cgctctcctc
     2581 actggtcgcc ggacggctcc gtgatcgcat tcctcagcta cggggaccgc gcatggaacg
     2641 tgtact
//
LOCUS       DASPWO010000450         5929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1249666, whole genome shotgun sequence.
ACCESSION   DASPWO010000450 DASPWO010000000
VERSION     DASPWO010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249666"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VEO00_10230"
     CDS             <1..853
                     /locus_tag="VEO00_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HYU58411.1"
                     /translation="LVAEVARAVGDPRRLALLYLLTVADAWATGPYARTPWRAALVRE
                     LVARVEAALTAGATATVVAGREAERQALLERHRALLEPRPLAVELRTSVEPEAVGTRS
                     YRLAVAALDRPGLLSKIAGALALSGLSILSAEVSTTDDGIALDVFVVEPAFHGDVDEE
                     RWRRFRGDLRRALEGRISLDYRVAEKRRHYPVGRDGIATEVTIDNQASEGFSVIEVSA
                     PDAVGLLYRITRALHELELDVHLAKVATYGDRVVDAFYVRDVYGRKVDEEHAREIERA
                     VVARLKE"
     gene            complement(894..1595)
                     /locus_tag="VEO00_10235"
     CDS             complement(894..1595)
                     /locus_tag="VEO00_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HYU58412.1"
                     /translation="MSGFRVGTSGFAYREWVGSFYPDGTRGRDMLSRYAERFDTVELN
                     TTFHHTPPPETVRTWLAEVPDGFRFTVKAHQRVTHWLKLMKPEESVPETLTTARAFGP
                     TLGAVLFQVPPRFRYREERLAAFLDALPADLRAAMEFRDPTWEAGRPLLAERGVAWVT
                     TDADARPAAELAAEPFAYARLRRTAYTDDDLRDWATRIRNALAAGRDVWCYLKHESKA
                     TEWARRLRDLVGTAP"
     gene            1643..2299
                     /locus_tag="VEO00_10240"
     CDS             1643..2299
                     /locus_tag="VEO00_10240"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HYU58413.1"
                     /translation="MAVERFTLETPRGPVSAAWHLPPGPRAIALVAHGAGVNMDHPLM
                     VGFAEGLEVSGMAALRFNFRYMEEGRKAPDGEASLREVYRAANDWTQERFPGKLHFAC
                     GKSLGGRIASMLVADGMPTDGLVFIGYPLHAPGKPEKLRADHLERIRVRMLFLWGTKD
                     PFARESALRPLIERLRPWARLHAVTGGDHSCVVRGVPEDETGRLLGGRAARFIQAAER
                     "
     gene            3274..3675
                     /locus_tag="VEO00_10245"
     CDS             3274..3675
                     /locus_tag="VEO00_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58414.1"
                     /translation="MAGESDSGVVRVSMWEEPSGRWRAQAETDPSLRASAETRDRCLA
                     RIRRLAIERTDARAGPLVLTVEVLPRLVGVAEAAQILGWDKRRLITYVDRGRFPEPVE
                     RLASGRIWRRADVEAYAARWRARATGRGARA"
     gene            3769..4152
                     /locus_tag="VEO00_10250"
     CDS             3769..4152
                     /locus_tag="VEO00_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HYU58415.1"
                     /translation="MGVKYLSEEWATAVQDALNSHEGFKNAAGSQTAKIQNVVTTTDG
                     ESKYYIKIEGGQVELKTGEIDAPEATITQDYDTAVALSKGELTGTAAYMSGKIKIAGD
                     LMKMMALQGALSQVNAATSSIEVEY"
     gene            complement(4194..4364)
                     /locus_tag="VEO00_10255"
     CDS             complement(4194..4364)
                     /locus_tag="VEO00_10255"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYU58416.1"
                     /translation="MHYLRHSDRGRVIGRGRAVHSGKRMGYGEAEILDDGGQLLARAS
                     ATFIVLPAPGAF"
     gene            complement(4474..5778)
                     /locus_tag="VEO00_10260"
     CDS             complement(4474..5778)
                     /locus_tag="VEO00_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HYU58417.1"
                     /translation="MQTEDPARTAPAPAAPVAGSPPPEMNPHEAVNWFFDRAADHVGL
                     DDEMRAVLRSSYRELMVQVPVRMDDGNVQVFSGYRVQHNGARGPYKGGIRYHPDADLN
                     EVRALAALMTWKTAIVDIPFGGAKGGVQCDPLTMSKAEKESLTRRFTGMIAYVIGVNR
                     DIPAPDVNTDAQTMAWMMDAYSSRYGYSPAIVTGKPVELGGSYGRDAATGRGVVDVLA
                     EAARDLPLDLDGATVAIQGFGNVGSWTARLIGSLGVRVVAVSDVTGGVHREDGLDVQA
                     LYDHAREARGVAGFPGADAMTNEDLLELGVDVLVPAALDRVIHQGNAGRVKARAILEA
                     ANHPVTPAADVVLQDRGILVIPDILANAGGVTVSYFEWAQNIQQFRWEEERVNEELGK
                     VMARSWKEVHARAMVDGIPLRLAAYAVAVEKVDRAERLRGYI"
     gene            complement(5832..>5929)
                     /locus_tag="VEO00_10265"
     CDS             complement(5832..>5929)
                     /locus_tag="VEO00_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HYU58418.1"
                     /translation="FTAALDRALNERGYILPGLGDFGDRLYGTEP"
BASE COUNT          838 a         2067 c         2138 g          886 t
ORIGIN      
        1 cctcgtggcg gaggtggccc gcgcggtcgg cgatccccgg cggctggcgc tgctctacct
       61 gctgacggtg gccgacgcgt gggcgacggg cccgtacgcc cggacgccct ggcgtgcggc
      121 gctggtccgc gaactcgtgg ccagggtcga ggccgcgctg acggccgggg ccacggcgac
      181 ggtcgtggcg gggcgggagg cagagcggca ggcgctcctc gagcgtcacc gcgcgttgct
      241 cgagccccgg ccgctggccg tcgagctccg cacgagcgtg gagccggagg cggtcgggac
      301 gcgatcgtac cgcctggccg tggcggcact cgaccggccc ggcctgctct cgaagatcgc
      361 cggcgccctg gccctatcgg gcctgtcgat cctgtcggcc gaggtctcga cgacggacga
      421 cgggatcgcc ctcgacgtgt tcgtcgtcga gccggcgttc cacggcgacg tggacgagga
      481 acgctggcgg cgcttccgcg gcgacctgcg ccgggcgctc gagggccgga tctcgctcga
      541 ctaccgcgtg gcggagaagc gccggcacta cccggtgggt cgcgacggga tcgccaccga
      601 ggtgacgatc gacaaccagg cctccgaggg gttctcggtg atcgaggtct cggcgccgga
      661 cgcggtcggc ctgctctatc ggatcacccg ggcgctgcac gagctggagc tcgacgtgca
      721 cctggcgaag gttgcgacct acggcgatcg cgtcgtcgac gcgttctacg tgcgcgacgt
      781 gtacggccgc aaggtggacg aggagcatgc ccgggagatc gagcgcgccg tcgtggcacg
      841 actgaaggag tgaccaccgg aagtggtcaa ggatctaaac gaatgtcggg tggtcaagga
      901 gctgtaccaa cgaggtcccg cagccggcgg gcccactcgg tcgccttcga ttcatgcttc
      961 agatagcacc acacgtcgcg gcctgcggcc agcgcgttgc ggatgcgggt cgcccagtcc
     1021 ctgaggtcgt cgtcggtgta tgccgtccgg cgcaggcggg cgtacgcgaa cggctcggcc
     1081 gccagttcgg ccgccggccg tgcgtccgcg tccgtcgtca cccaggccac gccgcgctcg
     1141 gcgagcaagg ggcgcccggc ttcccaggtc gggtcgcgga actccatggc agcccggaga
     1201 tcggcgggca atgcgtcgag gaacgcggcc agacgctcct cccggtagcg gaaccgcggc
     1261 ggcacctgga acagcaccgc cccgagcgtc ggcccgaagg cccgggcagt ggtcagggtc
     1321 tcgggcacgg actcctcggg cttcatcagc ttgagccagt gggtgacgcg ctggtgcgcc
     1381 ttcaccgtga agcggaaccc atccgggacc tctgccagcc aggtccgcac cgtctcgggc
     1441 ggcggggtgt ggtggaaggt cgtgttcagc tcgacggtgt cgaagcgctc cgcgtagcgg
     1501 ctgagcatgt cgcggccgcg cgtcccgtcc ggatagaacg agccaaccca ctcgcgatac
     1561 gcgaacccgg aggtgcccac ccggaacccg ctcacccgcc gattctccca ggagagctgt
     1621 tccgctggga taatccccgc tcgtggccgt cgagcgcttc accctggaga ccccgcgcgg
     1681 gccggtgtcg gccgcctggc acctgccgcc gggcccgcgc gccatcgccc tggtcgcgca
     1741 cggcgcaggg gtgaacatgg accacccgct gatggtcggg ttcgcggagg ggctcgaagt
     1801 ctccgggatg gccgcgctcc gcttcaactt ccgctacatg gaggaggggc gcaaggcgcc
     1861 cgacggcgag gcctcgctgc gtgaggtgta ccgcgcggcg aacgactgga cccaggaacg
     1921 gttcccgggg aagctccact tcgcctgcgg caagtcgctc ggcgggcgga tcgcctcgat
     1981 gctggtggcc gacggcatgc ccaccgacgg cctggtgttc atcggctacc cgttgcacgc
     2041 ccccggcaag cccgagaagc tgcgcgccga ccacctcgag cggatccggg tgcggatgct
     2101 cttcctgtgg gggacgaaag acccgttcgc ccgggagtcg gccctgcggc ccctgatcga
     2161 gcggctccgg ccgtgggccc gcctgcacgc ggtgaccggc ggcgaccact cgtgcgtggt
     2221 gcgcggcgtg ccggaggacg agaccggccg gctcctgggc ggccgggcgg cgcgcttcat
     2281 ccaggcggcc gagcggtagt cgggtggcag gaatcgtcgc cacggcgaac cctcggccat
     2341 ctcggcggag cgtcctcgct cgctgcggct cgatttcacc tggctcggac gagaacctgg
     2401 tgcggtccgc gggtcaggtc caagcggtga ggagatcttc gatgacgact agcggaaggc
     2461 tggctcgtcg cccacaagtc gctgcgttgg tggttgtctt gcttgggatg ttgctactgg
     2521 ccattgccct gtttcggctg atcccactct tcccggcctc gtccaatacc gtaggtgctg
     2581 acgccggatc tggggcgccg actcctcccg attgccctgc cggggggccc accgcttcat
     2641 cattcgtcgt tacggtcccg gagtctggtc cggttcattt ggagcccaat tgcctctatg
     2701 cgaggcaggg gagcccattt caggtcacct tccagaactt cgtgcgggcc acgcggagtg
     2761 gaaacggaat gatcgtcaat ctatcgatct accgaaacgc gggggaagca gcgacgcgga
     2821 gccagcgatt cgtaagagtc gatcgtagcc tggcgctctt tgttggcgaa gaggtcgccg
     2881 gtcctgcgag catcgtttac gaggtgccgc cactcgaagc gggtaccaac tacgtgcaat
     2941 cggactcctt gccagacttc ctgcacctga cgctggtcgt ctcctagggg gggtcggtct
     3001 ctccccgtaa gcaccgttcc gcgcgcgagc ggccggggcc gcccgcgccc ctcccgtcgt
     3061 cctcggcgcc cccgctctgg gcgacggtcc ccggcgtcca ggtccgggcc gtcgccggcg
     3121 agaaggacta ccgctgtccc ggctgccagc agacgatccg gaagggaacg ccgcacctgg
     3181 tggtggtccc cgccggcgac cccaccgccc gccgccactg gcacacaccg tgctggcgcc
     3241 gggagattcg ccgtcgcggg tagactgcgg gcggtggcgg gggaatcaga ctccggcgtg
     3301 gtgcgcgtct cgatgtggga ggagccctcc ggccgctggc gggcccaggc cgagaccgat
     3361 ccatcgctgc gggcttcggc cgagacccgc gaccgttgcc tggcccgcat ccgacggttg
     3421 gcgatcgagc gcacagacgc ccgcgcgggc ccgctggtcc tgaccgtcga ggtgctccca
     3481 cggctcgtcg gcgttgccga ggcggcgcag atcctcgggt gggacaagcg ccgtctgatc
     3541 acgtacgtcg accgcgggcg gttccccgag cccgtcgagc gactggcttc ggggaggatc
     3601 tggcggcggg ccgacgtgga ggcctacgcc gcgcggtggc gcgcccgcgc cacgggtcgc
     3661 ggcgcgcggg cctgacggac cgtcagacca cgcgccctgc tctggctata cttcccgcca
     3721 cggattccac cggggccgcg tgcccccccg gcagcgagga ggaacgacgt gggcgtgaag
     3781 tacctctccg aggagtgggc cacggccgtg caggacgccc tgaacagcca cgaggggttc
     3841 aagaatgccg ccggctcgca gacggcgaag atccagaacg tcgtgaccac caccgacggc
     3901 gagtcgaagt actacatcaa gatcgagggc ggccaggtcg agctgaagac gggcgagatc
     3961 gacgcccccg aagctacgat cacccaggac tacgacacgg cggtcgcgct gtcgaagggc
     4021 gagctgaccg gcaccgccgc ctacatgagc ggcaagatca agatcgccgg cgacctgatg
     4081 aagatgatgg cgctgcaggg cgccctgagc caggtcaacg ccgcgacgag ctcgatcgag
     4141 gtcgagtact agacaccacg gggggcttcg gccccccgtg ttccccccga aggctagaac
     4201 gctccgggcg cggggaggac gatgaacgtg gccgaggccc gggcaagcag ctggccgccg
     4261 tcgtccagga tctccgcctc cccgtagccc atccgcttcc cggagtgcac ggcgcggccc
     4321 cggccgatca cccggccgcg gtcgctgtgt cgcaggtagt gcacgttcag ctggagtccg
     4381 atccgcgatg cggtccctga gttcctcgag gtgttcgggt ccgaggccgg gcccggcgtc
     4441 ctcgtccatg acgccgccca aacgcgcctc ctcctagatg tagccgcgga ggcgttcggc
     4501 gcggtcgacc ttctccacgg ccacggcgta ggcggcaagt cggagcggga tgccgtccac
     4561 catggcccgg gcgtggacct ccttccagga ccgggccatc accttgccga gctcctcgtt
     4621 gacccgctct tcctcccagc ggaactgctg gatgttctgg gcccactcga agtaggagac
     4681 ggtcacgccg ccggcgtttg ccaggatgtc ggggatcacc aggatgccgc ggtcctgcag
     4741 caccacgtcg gccgccgggg tgaccgggtg gttggcggct tcgaggatcg cgcgcgcctt
     4801 gacccggccg gcgttgccct ggtggatgac ccggtcgagc gccgccggga ccaggacgtc
     4861 cacgccgagc tcgagcagat cctcgttcgt catggcgtcg gcgcccggga acccggccac
     4921 gccgcgcgcc tcgcgggcat ggtcgtagag cgcctgcacg tcgaggccgt cctcgcgatg
     4981 gacgccgccg gtgacgtcgg acaccgcgac cacccggacg ccgagcgagc cgatcagccg
     5041 cgccgtccac gagccgacgt tgccgaagcc ctggatggcg acggtggcgc cgtcgaggtc
     5101 gagcggcagg tcgcgggcgg cctcggccag gacgtcgacg acgccccgcc cggtggccgc
     5161 gtcgcgcccg taggagccgc ccagctcgac gggcttcccg gtgacgatgg ccggtgagta
     5221 cccgtagcgc gacgagtacg catccatcat ccaggccatc gtctgcgcgt cggtgttcac
     5281 gtcgggggcg gggatgtcac ggttgacccc gatgacgtac gcgatcatcc cggtgaaccg
     5341 gcgggtcagg ctctccttct ccgctttgct catggtcagc ggatcgcact gcacgccacc
     5401 cttcgccccg ccgaacggga tgtccacgat ggcggtcttc caggtcatca gcgcggcgag
     5461 cgcgcgaacc tcgttcaggt cggcgtccgg gtggtagcgg atcccgccct tgtatggccc
     5521 gcgcgcgccg ttgtgctgca cgcggtaccc gctgaagacc tgcacgttcc cgtcgtccat
     5581 gcgcacgggg acctgcacca tcagctcccg gtacgaactg cgcagcaccg cgcgcatctc
     5641 gtcgtccagc ccgacgtggt ccgccgcgcg gtcgaagaac cagttgacgg cctcgtgcgg
     5701 gttcatctcg ggcggcggcg agccagcaac gggcgcggcc ggagcgggag ccgtgcgggc
     5761 gggatcctcg gtctgcacgc ggggactcct tcgcctgggt gagcaagcgt accgccgggc
     5821 gagccggcgg gctagggctc ggtgccgtag agacggtcgc cgaagtcacc caggcccgga
     5881 aggatgtagc cgcgctcgtt cagcgcccgg tccagggctg cggtgaaca
//
LOCUS       DASPWO010000451         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_577073, whole genome shotgun sequence.
ACCESSION   DASPWO010000451 DASPWO010000000
VERSION     DASPWO010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577073"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VEO00_10270"
     CDS             <1..584
                     /locus_tag="VEO00_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU58419.1"
                     /translation="RMEMLEEQVEIVHRLWDREQPQTFAGKHYRLDGVRALPKPAQEP
                     HPPLIIGGRGGPRSARVAARWGDEYNVNGRTPEACRTVRAGLDRACQALDRDPASLRY
                     SLMTQVVAGADRRELEARAKRVMTVFGEPGDPSAYLANAAETGVVGTVNQVLDRLAAY
                     EEAGVERIMLQHLDHADLEMVALIGAEIIPQVA"
     gene            581..>2287
                     /locus_tag="VEO00_10275"
     CDS             581..>2287
                     /locus_tag="VEO00_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYU58420.1"
                     /translation="MTAIGRAGSGFVGRESELAELEAALGDAVEGRGRLVLISGEPGI
                     GKSRLAAELVRQAQARGIVVLSGRCWEAGGAPPYWPWVQALRPLVRELPAHRLASHLG
                     EGGPDVAQMLPEIRSALPDLAEPPSLDSDAARFQLFDSTAQFLLRAARDEPMVLVLDD
                     LHSADVPSLLLLRFVAPELSRAHVLVVATWRTGEMGIEHPLAEAVADVLRLPATRRLE
                     LAGFSERDVAAFVESTAGVTPSPQQVAAIRGETEGNPLFVQEVVRLLASEGMLEQVGR
                     GTSLPAIPQGVREVIGRRLLHISDECTRVLTLAAVLGREFTLEALRRMSELAADDLLE
                     VLEEAVSARVVEDVPGSLGWLRFSHVLIRDVLYEDVSGTQRLRLHRRAGTVLEELYRA
                     DPTPHLAELAHHFAQAAPAGEWAKAVEYARRAGDQAVDQLAYEEAVRVYGIALQALDL
                     EPSAEEETRCDLLLALGDAQARAGGLGARETFVAAADLAQRLGRPQALAKAALGYGGR
                     FTWSRAAGDARIVPLLEAARAALGERDDPLLVQVLGRLSCALRDRHEREPRWTLSRLA
                     VAM"
BASE COUNT          287 a          802 c          865 g          333 t
ORIGIN      
        1 tgcggatgga gatgctcgag gagcaggtcg agatcgtgca ccggctgtgg gaccgcgagc
       61 agccgcagac gttcgccggg aagcactacc ggctcgatgg cgtccgggcg ctgccgaagc
      121 cggcgcagga gccgcacccg ccgctgatca tcggcggccg gggcggcccc cgctcggccc
      181 gcgtcgcggc gcgatggggc gacgagtaca acgtcaacgg ccggacgccg gaggcctgcc
      241 gcacggttcg ggccggcctg gaccgcgcct gccaggccct ggatcgcgat ccggcgtcgc
      301 tccggtactc gctgatgacg caggtggtcg ccggggcgga ccgacgcgag ctcgaggccc
      361 gagccaagcg ggtgatgacg gtcttcggcg agccaggaga cccctccgcg tacctggcga
      421 acgccgcgga gaccggggtc gtcggaaccg tgaaccaggt cctcgaccgc ctggcggcgt
      481 acgaggaggc gggcgtggag cggatcatgc ttcagcacct cgaccacgcc gaccttgaga
      541 tggtcgccct gatcggcgcc gagatcatcc cgcaggtcgc gtgaccgcga tcggccgcgc
      601 cggcagcgga ttcgtcggcc gcgagtccga gctggccgag ctggaagcgg ccctcggcga
      661 cgcggtcgag ggccgcggcc ggctggtcct gatctccggc gagccgggga tcggcaagag
      721 ccggctggcc gccgagctgg ttcgccaggc gcaggcacgc ggcatcgtgg tcctgtcggg
      781 tcggtgctgg gaggccgggg gtgcgccgcc ctactggccg tgggtccagg cgcttcgccc
      841 gctcgttcgc gagctcccgg cgcaccggct ggcgagccac ctcggggaag gcgggccaga
      901 cgtcgcgcag atgctcccgg agatccgatc ggcgctgccc gatctggcgg agcctccgtc
      961 gcttgattcc gacgccgctc gattccagct gttcgactcc actgcgcagt tcctgctgcg
     1021 cgccgcccgc gacgagccaa tggtgctggt ccttgacgac ctgcactcgg cggacgttcc
     1081 ctctctgctc ctgctgcgat tcgtggcccc ggagctctct cgggcgcacg ttctcgtcgt
     1141 cgccacgtgg cgcaccgggg agatgggcat cgaacatccg ctggcggagg ccgtagcgga
     1201 tgtcctccgg ctccccgcga cgcgccggct ggagctggcc gggttctcgg agcgggacgt
     1261 ggctgcgttc gtggaatcga ccgccggggt gacgccctca cctcagcagg tcgcggcgat
     1321 ccgcggcgaa accgagggga acccgctgtt cgtccaggag gtcgtccgcc tactggcctc
     1381 cgaagggatg cttgagcagg tcggccgcgg gacctcgctc ccggcgatcc cgcagggcgt
     1441 gcgcgaagtg attggccgcc gcctgctgca catctcggac gagtgcaccc gcgtgctgac
     1501 cctcgccgcg gtcctcgggc gtgagttcac cctggaggcc ctccgtcgga tgagcgagct
     1561 cgcggccgat gacctgctgg aagtgctgga ggaggccgta tcggcccgcg tggtcgagga
     1621 tgttcccggc tcgctcggct ggctgcgctt ctcccacgtg ctgatccggg atgtcctgta
     1681 cgaggacgtg tcggggaccc agcggctgcg gctgcaccgc cgcgctggga ccgtgctcga
     1741 ggagctgtat cgcgcggacc cgacgccaca cctcgcggag ctcgcgcatc acttcgcgca
     1801 ggccgcgccc gccggagagt gggcgaaggc cgtcgagtac gcccgccgcg ccggggatca
     1861 ggcggtcgac cagctcgcgt acgaggaggc cgtccgcgtg tacgggatcg cgttgcaggc
     1921 gctcgacctg gagccctccg ccgaggagga gacacgatgc gacctgctcc tggccctcgg
     1981 ggatgcccag gctcgggcgg gagggctcgg tgccagagag accttcgttg cggcggccga
     2041 cctcgctcaa cgcctcgggc ggccccaggc cctcgccaag gccgcgctcg ggtacggcgg
     2101 tcggttcacc tggtccaggg cggccgggga cgcgcggatc gtcccgctcc tcgaggccgc
     2161 tcgcgcggcg ctgggggagc gggacgaccc gctgctggtc caggtgctgg ggcggctttc
     2221 gtgcgccctg cgcgaccgcc atgagcgaga gcctcgctgg accctcagcc ggctggcggt
     2281 cgcaatg
//
LOCUS       DASPWO010000452         3171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1249726, whole genome shotgun sequence.
ACCESSION   DASPWO010000452 DASPWO010000000
VERSION     DASPWO010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249726"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VEO00_10280"
     CDS             <1..499
                     /locus_tag="VEO00_10280"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HYU58421.1"
                     /translation="RRFGLVAAVLDRAGIPVGRGELYGAACGGLLVDDPGADLAVAAA
                     LASSRVGAPPPPATAFVGEVSLTGAVRPVPGLAQRLAAAAATGIRHVVGPPGDGGFGG
                     VARPGEGRARPRPASEPPTGGGDRSGVELVPVRHLRDAVGWARRARASEPEPAGQAGV
                     RRVGG"
     gene            599..1087
                     /locus_tag="VEO00_10285"
     CDS             599..1087
                     /locus_tag="VEO00_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HYU58422.1"
                     /translation="MFRKGDKVVYPHHGAAVIQDIRNVELLGERRKYLVLKLAYGGDL
                     TLKVPVDNTEEVGLREVVSRREVKKVMNVLREDESKMSTNWSRRFKTNWEKLRSGDIY
                     AVAEVVRDLSIRERSKGLSAGEKRMLAKARQILVSELTFAVDATEEKAEAMIDKVLEE
                     SH"
     gene            1136..2191
                     /locus_tag="VEO00_10290"
     CDS             1136..2191
                     /locus_tag="VEO00_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HYU58423.1"
                     /translation="MSGERRHSGLVVEGVRLIVLGLFAAGGYAIAEALDRQRRDLAIM
                     VILGVLVGYVAGGVFGRGTAAAVSAAERSFRTASAAELLAGTAGLIAGLLIAFLLSFP
                     LLRLPAEAGYPAVAFAYLVLGYLGERVARAKRDDLFALFGLKSRAAASGSGDVTVVDT
                     SALVDGRVVDVVRAGFLSGTLLVPGMVLRELQSIGDASDPARRSRGRRGLDAVRELQV
                     HPDVDVVVIEDPAGAGDVDAALVRLAREHRAGLLTNDTQLQKVAGAVGVPVRSVARLA
                     GSLRVPVSGGDDLQIGLSREGREHGQGVGFLDDGTMVVVEGGSGLVGSSVRVTVTNVI
                     DTANGRMLFARLPVAAS"
     gene            2188..2892
                     /gene="ispD"
                     /locus_tag="VEO00_10295"
     CDS             2188..2892
                     /gene="ispD"
                     /locus_tag="VEO00_10295"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HYU58424.1"
                     /translation="MRAAALLLAGGRGERLADVPKALVHVGDRSLVEWAVAAVEAAPE
                     VEGFVVVLPAAAGLAVEAERIARAARSPKLLAPVPGGRERQDSVAAGLAALDRGFDAV
                     LCHDVARPFASPALFARVLAALSGADGAVPVVPLPDTVKRVQGGLVVETVDRDALAAA
                     QTPQAFRRSALEEAHRRAEAEGARATDDAALLERAGFRVAAVPGEPGNFKVTLPADLE
                     RARALAAAGALGAPGR"
     gene            2889..>3171
                     /locus_tag="VEO00_10300"
     CDS             2889..>3171
                     /locus_tag="VEO00_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HYU58425.1"
                     /translation="MSERRFGLGFDVHPPDPSRPLVLGGVAFPGEAGLGGHSDADVVC
                     HALAQALLGAAALGDLGDHFPDTDPAFAGLPGLDLLRETVRRVSRAAYRP"
BASE COUNT          356 a         1107 c         1241 g          467 t
ORIGIN      
        1 gcggcgcttc ggcctggtcg ccgcggtgct cgaccgggct ggcatcccgg tcggccgcgg
       61 cgagctgtac ggggccgcct gcggtggcct gctcgtcgac gacccgggcg ccgacctcgc
      121 cgtggcggcg gccctggcgt cgtcgcgggt cggggccccg ccgcctccgg ccacggcctt
      181 cgtcggcgag gtgtcgctga ccggcgcggt ccgcccggtt ccgggactcg cccagcggct
      241 ggcggcggcg gccgcgaccg gcatccgcca cgtcgtgggt ccgccggggg acggggggtt
      301 tggcggggta gctcgccctg gggagggacg cgcccggcca cgtccagcga gcgaaccccc
      361 cacggggggc ggcgaccggt cgggagtcga gctggtgccg gtccgccacc tgcgggatgc
      421 ggtcggctgg gccaggcggg cgagggccag tgagcccgaa cccgcaggtc aggcgggggt
      481 gaggagggtg ggggggtaga atagggcctc tgacctgcgc ttttggtttg ccggtgggcc
      541 cggtcggtgg tatccttgcg atcggtcggc ccatccgttc ctgttcatag gggatgtcgt
      601 gttccgcaag ggagacaagg tcgtctatcc gcaccacggt gccgccgtca ttcaggacat
      661 tcggaacgtc gaactgctcg gtgaacgaag gaagtatctc gtcctcaagc tcgcctatgg
      721 cggggacctg acactgaagg ttcctgtcga caacaccgag gaggtcggcc tccgggaggt
      781 cgtctccagg cgcgaggtca agaaggtcat gaacgtcctg cgcgaggacg agtccaagat
      841 gtccactaat tggagccgcc gcttcaagac caactgggag aagctgcgct ccggagacat
      901 ctacgccgtc gcggaggtcg tgcgcgacct ctcgatccgc gagcgctcca agggcctttc
      961 tgcgggggag aagaggatgc tggccaaggc ccgccagatc ctggtctccg agctgacctt
     1021 cgcggtggac gccaccgagg agaaggccga ggccatgatc gacaaggtcc tcgaggagtc
     1081 gcactaactc tcgggaacgt ttgcatcccg cccccgagcg gggcacggta accaggtgag
     1141 cggcgagcgc cggcactccg ggctcgtggt cgaaggtgtg cgcctgatcg tcctcgggct
     1201 gttcgccgcc ggcggctacg ccatcgccga ggccctcgac cggcagcgcc gcgacctggc
     1261 gatcatggtc atcctcgggg tgctggtcgg gtatgtggcc ggtggcgtgt tcggacgcgg
     1321 gacggccgca gcggtgtcgg ccgccgagcg gtcgttccgg acggcaagcg cggcggagct
     1381 gctcgccggt accgccggac tgatcgccgg cctgctgatc gcattcctgc tgtcgttccc
     1441 gctgctccgc ctgccggccg aggccggcta cccggccgtc gccttcgcct acctggtgct
     1501 cggctacctc ggggaacggg tcgcgcgtgc caagcgcgac gacctcttcg cgctgttcgg
     1561 gctgaagtcg cgcgccgccg catcggggtc gggtgacgtc accgtcgtcg acaccagcgc
     1621 cctcgtcgac gggcgcgtgg tggacgtcgt ccgcgccggg ttcctgagcg gcacgctgct
     1681 cgtccccggg atggtgctcc gcgagctgca gtcgatcggc gacgcatccg atccggcccg
     1741 gcggtcgcgc ggccggcgag ggctcgacgc ggtgcgcgag ctgcaggtcc accccgacgt
     1801 ggacgtggtg gtgatcgagg atccggccgg tgccggagac gtcgacgcgg ccctcgtccg
     1861 gctggcgcgg gagcaccgcg cggggctgct gacgaacgac acgcagctgc agaaggtggc
     1921 gggcgccgtc ggggtgccgg tgcgctcggt ggccaggctg gccgggtcgc tgcgcgtccc
     1981 ggtcagcggg ggggacgacc tgcagatcgg cctgtcgcgc gagggacgcg agcacggcca
     2041 gggcgtcggg ttcctcgacg acggcacgat ggtcgtcgtg gagggcggga gcggcctggt
     2101 cggctccagc gtccgggtca ccgtgacgaa cgtcatcgac accgcgaacg ggcggatgct
     2161 cttcgcccgg ctgcccgtgg cggcctcgtg agggcggccg ccctcctgct ggccggcggc
     2221 cggggggagc ggctggccga cgtgcccaag gcgctcgtgc acgtcggcga ccgatccctg
     2281 gtcgagtggg ccgtggccgc cgtcgaggcg gcccccgagg tggaagggtt cgtggtcgtc
     2341 ctccccgcgg cagccggcct ggccgtcgag gcggagcgca ttgcccgcgc cgcgcgctcc
     2401 ccgaagctgc tcgccccggt tccggggggc cgggagcgac aggactcggt cgccgcaggg
     2461 ctcgccgcgc tggatcgcgg cttcgacgcg gtgctctgtc acgacgtcgc ccggccgttc
     2521 gcctccccgg ccctgttcgc ccgcgtgctg gctgccctgt cgggcgccga cggggccgtt
     2581 cccgtcgtgc cgctccccga caccgtcaag cgggtccagg gcggcctggt cgtggagacg
     2641 gtggaccgcg acgcgctggc ggccgcccag acgccgcagg ccttccggcg atcggccctg
     2701 gaggaggcgc accgccgtgc cgaggccgag ggtgcccggg ccaccgatga cgcggcgctc
     2761 ctggagcgcg ccgggttccg ggtggcggcc gtgccgggcg agccggggaa cttcaaggtc
     2821 accctgcccg cggacctcga gcgggcccgg gcactcgccg ccgccggcgc tctcggggca
     2881 ccgggccggt gagcgagcgc cggttcggtc tcggcttcga cgtgcatccg cccgacccct
     2941 cccgtccgct cgtgctcggc ggggtggcct ttccgggcga ggccgggctc ggtggccatt
     3001 cggacgccga cgtcgtttgc cacgcgctgg cgcaggccct gctcggcgcg gcggcgctgg
     3061 gggacctcgg ggaccacttc ccggacaccg atccggcctt cgccggcctt cccggcctgg
     3121 acctgctgcg ggagacggtg cggcgcgtct ccagggcggc gtaccggccc g
//
LOCUS       DASPWO010000453         4354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_235594, whole genome shotgun sequence.
ACCESSION   DASPWO010000453 DASPWO010000000
VERSION     DASPWO010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235594"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VEO00_10305"
     CDS             <1..387
                     /locus_tag="VEO00_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011761006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HYU58426.1"
                     /translation="VRMTAINSAIQVDLSGQVCADSIGWRLYSGVGGQMDFIRGAALA
                     REGRAIIALPSTAAGGTVSRIAPFLSEGAGVVTTRAHVKTVVTEWGVAELYGRSIAER
                     ARALIDIAHPDFREDLEAHARETHYL"
     gene            464..808
                     /locus_tag="VEO00_10310"
     CDS             464..808
                     /locus_tag="VEO00_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58427.1"
                     /translation="MRRLTSRTARWLALGAVGLAAIGIGAGIAIAAGGDRDAPLTGSD
                     LDRAVAAALEHTGGGTVVETEVGDDGAAYGVEIRLEDGRQVEVSLDQHFRVIGQESDE
                     EAPGETDAGADG"
     gene            complement(816..902)
                     /locus_tag="VEO00_10315"
     tRNA            complement(816..902)
                     /locus_tag="VEO00_10315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(866..868),aa:Ser,seq:tga)
     gene            936..1877
                     /locus_tag="VEO00_10320"
     CDS             936..1877
                     /locus_tag="VEO00_10320"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYU58428.1"
                     /translation="MFRRTGRVTWLAATVALAVLAPACGPAPTAGRPLADDDTVVVAS
                     FEFSESVLLAEIYAQAIEAAGIRVERELGLGARELVAPALQAGLVEFVPEYAGSALAF
                     AGLGATPVTADPQATHDALTAVMARRGVTVLDAAPAENQNGIAVTAATAAEYGLTTIS
                     DLVPLAPSFTFGGSVECPQRPLCLPGLESVYGLTFERFVPLDASGPLTAAALRSGQVD
                     VGLMFTTDGRVEANRLVFLADDRGLQPAENVTPVVRRSVVDHLGRPLVDAVNAVSGLL
                     TTEELRSLNALVAIEGRAPAMVAATWLSARGLTRSVG"
     gene            1923..3581
                     /locus_tag="VEO00_10325"
     CDS             1923..3581
                     /locus_tag="VEO00_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HYU58429.1"
                     /translation="MSVGEESRQLRRGRARRPSGQPPPLPHHLGRSGKLWLQLSLYVV
                     AVTGLALFGPFQKIFEQFDTERLRWMATLRSNWLTNVMLVVNGTASTWTIRALRWATI
                     LTLVGFRRWRHLFVFLGATLAFEFVALQISEITQRPRPLGVTILAGWQGFSMPSRPMG
                     GLAVTLVGMAFALVVAGRPRYLAKVAIVVVLALVFLARLYLAVDQPTSAGFGTVLGVA
                     LGLALFRLFTPTDVFPVSYRRGKSAHLDVGGPRGEAIVKAVRDQIGLDVLEAKPVGLE
                     ASGGSTPLRLRVGASGDEPERYLFAKLYAKSHVRADRWYKLGRSILYGALEDETPFQS
                     VRRFVEYEDYTLRLMYEGGLPAPKPYGIVEITPEREYMIVMEFFDGAVELGDAEIDAG
                     VVDQGLRLIREMWDMGLAHRDVKPANLMVREGQLKLIDVFFVQVRPSPWRQAVDLGNM
                     MLVLGLRTDAESVYRQALKYFSPVEIGEAFAATRGVASPTQLRAFLKRDGRDLLRRFR
                     ELAPPSPPVAIQRWSLRRVLLTIGALLGVLLAVLTLTSNWAVFT"
     gene            3578..4159
                     /locus_tag="VEO00_10330"
     CDS             3578..4159
                     /locus_tag="VEO00_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58430.1"
                     /translation="MSLRARGSIAAGALAAVLLPACGVAHEVRASCTAADPTLTILSA
                     QAVPSATMLPCIKEFPAGWTYEGEDVRSNRATFWLANDRAGFRAVQVDLTGECDVGSA
                     VEVAPAPDEAGTRRFEEPLALSPAYQANRYYRFPGGCVTYRFRFRAGASTTLALEVDE
                     ALSFRPRAPLVEHVREEFGQILCGAGAPPCIGE"
BASE COUNT          582 a         1531 c         1564 g          677 t
ORIGIN      
        1 gtccggatga cggcgatcaa ctccgccatc caggtcgacc tgtcggggca ggtgtgcgcg
       61 gactcgatcg gctggcgcct ctacagcggg gtcggcgggc agatggactt catccgcggt
      121 gccgccctgg cccgcgaggg cagggcgatc atcgcgctcc cctcgaccgc cgccggcggg
      181 acggtctcgc ggatcgcccc gttcctgtcg gagggtgccg gcgtggtgac cacccgcgcc
      241 cacgtcaaga cggtggtcac ggagtggggg gtggcggagc tctacggccg ctcgatcgcc
      301 gagcgcgccc gggcgctgat cgacatcgcc caccccgact tccgcgagga cctcgaagct
      361 cacgcccgcg agacgcacta cctctgaggc acgccgctgc gggactcacc gtcatctcat
      421 ccgcgatggg gcacgatccg ccaggacgaa gaagggaggc agcatgcgaa ggctgacgag
      481 caggacggcc cgctggctcg ccctgggcgc cgtgggactg gccgcgatcg ggatcggcgc
      541 cgggatcgcc atcgccgcgg ggggcgaccg cgacgccccg ctcacgggct cggacctgga
      601 ccgggcggtg gcggcggccc tggagcacac cggcggcggg accgtcgtcg aaaccgaggt
      661 cggcgacgac ggggccgcct acggggtcga gatccgtctg gaggacgggc ggcaggtcga
      721 ggtgagcctt gaccagcact tccgggtgat cggtcaggag tccgacgagg aagccccggg
      781 cgagaccgac gcgggcgcgg acggctgaca gccggcgggg gaaggaggat tcgaacctcc
      841 ggagccctcg cgggctctgc ggttttcaag accgccccgt tcgtccgctc cggcattccc
      901 ccctggagcc gagtgtaggc tcgggcaagg cttccatgtt tcggcgaacc gggcgcgtca
      961 cgtggcttgc tgcaacggtc gccctcgccg tgctggcacc cgcgtgcggg cccgcgccca
     1021 ccgcgggccg gccgctggcc gacgacgaca ccgtcgtggt cgcctcgttc gagttctccg
     1081 agagcgtcct gctcgccgag atctacgctc aggcgatcga ggcagcggga atccgggtcg
     1141 agcgagagct cgggctcggg gcccgtgagc tcgtggcgcc cgcgctgcag gcagggctcg
     1201 tcgagttcgt gcccgagtac gcggggagcg cgctggcatt cgccggcctt ggagccaccc
     1261 cggtcacggc cgacccgcaa gccacccacg acgccctcac ggcagtgatg gcgcggcgtg
     1321 gggtgacggt cctcgacgcc gcgcccgccg agaaccagaa cgggatcgcc gtgaccgccg
     1381 cgacggccgc cgagtacgga ctgacgacca tcagcgacct ggtcccgttg gcgccctcct
     1441 tcacgttcgg cgggtcggtg gagtgcccac agcggccact ctgcctgccc ggtctggagt
     1501 cggtgtacgg actgacgttc gagcgtttcg tgccgctcga cgcgagcggt ccgctgaccg
     1561 cggcggccct gaggagcggg caggtggacg ttggactgat gttcacgacc gacggacgag
     1621 tcgaagcgaa ccggttggtc ttcctcgccg acgaccgcgg cctgcagccc gcggagaacg
     1681 taacgccggt cgtccggcgg tccgtggtcg accacctcgg tcgtccgctg gtggacgccg
     1741 tgaacgccgt gtcggggctg ctcaccaccg aagagcttcg aagcctgaac gcgctggtcg
     1801 ccatcgaggg ccgcgcaccc gccatggtcg cggcgacgtg gctcagcgcg cgcggcctga
     1861 cgcggtcggt gggatagcat cgggagaccc tcgaaagcgg agaccgacac ggccatcggg
     1921 cgatgagcgt cggcgaagag tcacggcaac tccgccgtgg tcgggcccgc cggccatcag
     1981 gccagccgcc gccgctgcca caccacctcg ggcgaagcgg caagctctgg ctccagctgt
     2041 ctctctatgt ggtggccgtc accgggttgg ccctgttcgg cccgttccag aagatcttcg
     2101 agcaattcga caccgaacgg ctgcggtgga tggccacgct ccgctcgaac tggctgacca
     2161 acgtgatgct ggtcgtgaac ggcacggcct cgacctggac catccgggcg ttgcgctggg
     2221 cgacgatcct gacgctggtc ggcttccgtc gatggcggca cctgttcgtg ttcctcggcg
     2281 ccaccctggc gttcgagttc gtcgccctcc agatctcgga gatcacgcag cgccctcgcc
     2341 cgttgggcgt cacgatcctg gccggctggc aggggttctc catgccgtcg cgcccgatgg
     2401 gcggcctggc ggtgacgctg gtcgggatgg ccttcgccct ggtcgtggcc ggccggccac
     2461 gctacctcgc caaggtggcg atcgtggtcg tcctcgcgct ggtgtttctg gcgcggctgt
     2521 atctcgcggt cgaccagccc accagcgcgg ggttcggaac ggtgctcggc gtggccctgg
     2581 ggctcgctct cttccggttg ttcaccccga ccgacgtctt ccccgtctcc taccgtcggg
     2641 gcaagtccgc gcacctggac gtgggtggac cgcgcggaga ggccatcgtc aaggcggttc
     2701 gcgaccagat cggcctggat gtgctggagg cgaagccggt cgggctcgag gcctccgggg
     2761 gctcgacccc cctgcgactg cgggtgggcg cctcgggcga cgagcccgag cgctatctgt
     2821 tcgccaagct ctacgcgaag agtcacgtgc gcgccgaccg ctggtacaag ctcgggcgtt
     2881 cgatcctcta cggcgcgctc gaggacgaga cgcccttcca gtccgtccgc cgcttcgtcg
     2941 agtacgagga ctacacgctg cgcctgatgt acgagggagg gcttccggcc ccgaagccgt
     3001 acgggatcgt cgagatcaca cccgagcggg agtacatgat cgtgatggag ttcttcgacg
     3061 gcgcggtcga gctcggggat gcggagatcg acgcgggcgt ggtcgaccag gggctccggc
     3121 tgatccgcga gatgtgggac atgggcctcg cgcaccgtga cgtgaagccc gccaacctga
     3181 tggtgcgcga aggacagctg aagctgatcg acgtgttctt cgttcaggtt cgcccgtcgc
     3241 cgtggcggca ggccgtggat ctcgggaaca tgatgctcgt cctcgggttg cgcaccgacg
     3301 cggagagcgt gtaccggcag gcgctgaagt acttcagccc cgtggagatc ggcgaagcgt
     3361 tcgccgccac ccggggcgtg gccagcccga cgcagctccg cgcgttcctg aagcgggacg
     3421 gccgcgacct gctgcggcgg ttcagagagc tggcgcctcc ctccccgccg gtcgccatcc
     3481 agcgctggtc cctgcggcgc gtcctcctga cgatcggtgc cctgttgggg gtcctgctcg
     3541 ccgtcctcac gctcacgtcc aactgggcgg tgttcacgtg agcctccgtg cgcgcggatc
     3601 gatcgccgcc ggcgcccttg ccgccgtcct gctgcccgcc tgtggcgtcg cgcacgaggt
     3661 tcgcgcgtcc tgcaccgccg ccgaccccac gctgaccatc ctgtccgccc aggccgtccc
     3721 gtcggccacg atgcttccgt gtatcaagga gttccccgcc gggtggacct acgagggtga
     3781 ggacgtccgc agcaaccggg cgacgttctg gctggcgaac gatcgcgcag ggttccgcgc
     3841 ggtccaggtc gacctgaccg gcgagtgcga cgtggggagc gccgtcgagg tcgctccagc
     3901 accggacgag gcgggcaccc ggcggttcga ggagccgctc gccctcagcc ccgcctacca
     3961 ggccaaccgg tactaccggt tcccgggggg ctgcgtcacg taccgcttcc ggttccgggc
     4021 cggcgcctcc accaccctgg cactcgaggt cgacgaggcg ctgtcgttcc gcccccgggc
     4081 gccgctcgtg gagcacgtgc gcgaggagtt cgggcagatc ctctgtggcg cgggagcccc
     4141 accgtgcatc ggggagtagc ggcgggcccc gaacccgccg ccctcgccaa ccatcgccgg
     4201 gtgctcgtgt actccatcgt tctgctcgtc gctctgacgc tcgtcttcct cgcggttggc
     4261 cgacatccgc cgcgggaggc gccggcgacg acgatcgcct tcgtcgggga cctcgaccgg
     4321 tcgacgtaca gcgctctcga ggatgtccgc aacg
//
LOCUS       DASPWO010000454         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_406876, whole genome shotgun sequence.
ACCESSION   DASPWO010000454 DASPWO010000000
VERSION     DASPWO010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406876"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VEO00_10335"
     CDS             complement(<1..990)
                     /locus_tag="VEO00_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYU58431.1"
                     /translation="MGPLEGLVYGFGVALTPANLFACFVGVLIGTIVGVLPGIGPVGA
                     MALLLPSTFALSPTTALIMLAGIYYGAMYGGSTTSILVNVPGEAASVVTTLDGYQMAR
                     KGRAGAALAVSAVGSFVAGSLGVVGLMLFAAWLADAALQFGPPEYFALSVTGLAILSR
                     LSGGSVLVAFAMVGLGLAIGTVGMEPISGYRRFTFGSTQLSQGLELVPIIMGLYGVAE
                     VLVLAEEGLRRSEIVKVRMRELFPTREEWRRSSWPIARGSVLGFLTGLVPGPATVLAT
                     FISYTVERRLSKTPERFGTGAIEGVAGPEAANNGATAGAMVPLLSLGIPFSPAT"
     gene            complement(998..1441)
                     /locus_tag="VEO00_10340"
     CDS             complement(998..1441)
                     /locus_tag="VEO00_10340"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HYU58432.1"
                     /translation="MRERAWGLGLLVASGTYLAASLGLPLGDAAKPGPGFFPVGVGVF
                     LCLAAVALMVGRRRGAPGDAGVASLSRAARARVFATIVGLVAFCLLLPWLGYPVCAFL
                     FIALLLRRLGGARWPGVVVTAVLGALVSYYVFGVLLGVPFPAGPF"
     gene            complement(1444..2397)
                     /locus_tag="VEO00_10345"
     CDS             complement(1444..2397)
                     /locus_tag="VEO00_10345"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYU58433.1"
                     /translation="MIRIALALLVVLASAVPAVAQYPDRPVTAIVGYPAGGLADVVLR
                     AVAEGMKKRFPKGIAVVARPGAGGTIGASEVIKSKPDGYTVGIMPITNLVIQPQVMDL
                     PYRTPDDCIPVISLVSWYPLLVVKHDAPWKTAQEFVAAAKANPGKFRVGSPGEATSSH
                     LNLEEMMRLSDARMTHVPFSGWGESSPALLGGHIEGLIAQPGEVRPLVEGKRMRVLAV
                     FQPARNPVFPDAPTWKEIGYPVHNGSTFFLVLPNGTPPDVVRYIHDAAKAVMDDPAFA
                     NFVKARVIDVDYRAGDKLRADLWQEYKAHTEILKRLGMLRK"
BASE COUNT          453 a          888 c          898 g          316 t
ORIGIN      
        1 cgtggccggc gagaacggga tgccgagcga gagcaggggt accatcgcgc cggcggtggc
       61 gccgttgttg gccgcctccg gtcccgccac gccctcgatg gcgccggtgc cgaagcgctc
      121 gggcgtcttc gataggcgcc gctccaccgt gtaggagatg aatgtcgcca gcaccgtggc
      181 cggtcccggc accaggccgg tgaggaaacc caggaccgaa ccccgcgcga tcggccacga
      241 cgaccgccgc cactcctcgc gtgtggggaa cagctcgcgc atccggacct tcacgatctc
      301 gctgcgacgg agcccttcct cggcgaggac gagcacctcg gccacgccgt agaggcccat
      361 gatgatcggc accagctcca gcccctgcga gagctgcgtc gagccgaacg tgaagcgccg
      421 gtagcccgag atcggctcca tgcccacggt gccgatagcc aggccgaggc cgaccatggc
      481 gaacgcgacg agcaccgacc cgcccgacag gcgcgacagg atggcgaggc cggtcaccga
      541 gagggcgaag tactcgggcg gcccgaactg cagcgcggca tcggcgagcc aggcggcgaa
      601 cagcatgagg cccaccaccc cgagcgagcc tgctacgaac gagccgaccg cggacaccgc
      661 gagcgccgcg ccggcgcgtc ccttgcgcgc catctgatag ccgtcgagcg tcgtgaccac
      721 cgaggccgcc tcgccgggaa cgttgacgag gatcgacgtc gtcgatcctc catacatcgc
      781 cccgtagtag atgccggcga gcatgatcag cgccgtggtc ggcgacaggg cgaacgtcga
      841 gggcagcagc agggccatgg caccgaccgg accgatgccg ggcagcaccc cgacgatcgt
      901 cccgatgagg acgccgacga agcaggcgaa cagattggcg ggcgtcagcg cgacgccgaa
      961 accgtagacc agaccctcga ggggacccac gcccagtcta gaacgggccg gcggggaatg
     1021 ggacgccgag gagaacgccg aagacgtagt aggacacgag cgcgcccagg accgcggtga
     1081 ccacgacgcc cggccatcgc gcgccgccca gacgccgcag cagcagcgcg atgaacagga
     1141 acgcgcacac cgggtagccc aaccacggca gcagcaggca gaaggcgacg agccccacga
     1201 tcgtggcgaa tacccgcgcg cgtgcagcgc gcgacaggct cgccacgccg gcgtcgcccg
     1261 gagcgccgcg ccgacggccg accatcagcg cgacggcggc caggcagaga aacacgccca
     1321 ctccgaccgg gaagaatccg ggcccgggct tggccgcgtc cccgagcggg aggccgaggg
     1381 acgccgccag gtacgttcca ctggcgacga gcaagccgag cccccaggcg cgctcgcgca
     1441 tcgctacttt cggagcatcc cgaggcgctt gaggatctcg gtgtgcgcct tgtactcctg
     1501 ccagaggtcg gcgcgcagct tgtcgccggc ccggtagtcg acgtcgatga cgcgcgcctt
     1561 cacgaagttg gcgaaggccg ggtcgtccat cacggccttg gcggcgtcgt ggatgtagcg
     1621 cacgacgtcc gggggcgtcc cgttgggcag gacgagaaag aacgtcgagc cgttgtggac
     1681 agggtagccg atctctttcc aggtcggcgc gtcggggaag accggattgc gcgcgggctg
     1741 gaacacggcg agtacgcgca tgcgcttgcc ctcgaccagc ggtcgcacct cgccgggttg
     1801 agcgatcagg ccctcgatgt gacccccgag cagggccgga ctgctttctc cccagcccga
     1861 gaacgggacg tgggtcatcc tggcgtccga cagtcgcatc atctcctcga ggttcagatg
     1921 gctcgaggtc gcctcacccg gcgagcccac gcggaacttg ccgggattgg ccttggcggc
     1981 cgcgacgaac tcctgggcgg tcttccaggg cgcgtcgtgc ttgacgacta gcaacgggta
     2041 ccacgacacc aggctgatca ccggaatgca gtcgtcgggc gtccggtacg gcaggtccat
     2101 cacttgaggc tggatcacga ggttcgtgat cggcatgatg ccgaccgtat agccatccgg
     2161 cttggacttg atcacctcgc tggcgccaat ggtgcccccg gcgccgggcc gggccacgac
     2221 cgcgatcccc ttcgggaacc tcttcttcat cccctccgcg accgcgcgca ggacgacgtc
     2281 ggcgagccca cccgctggat agccgacgat cgcagtaacc ggccgatccg ggtactgggc
     2341 gacggccgga accgcagagg cgagcacgac gagcagcgcg agcgcgatgc ggatcatggt
     2401 gtcctcctga gcagcctggc gagggtcccc gggagtctag tcgggaacgc ggacgcgatc
     2461 aaggcgccgg ggaacgcgac tcgccccgga tctcgtctac gagcggcagg cgcgaaagtt
     2521 cgcgtagtcg ccggcggcgg tggacctggt gtcgt
//
LOCUS       DASPWO010000455         3692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1166599, whole genome shotgun sequence.
ACCESSION   DASPWO010000455 DASPWO010000000
VERSION     DASPWO010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166599"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1813)
                     /locus_tag="VEO00_10350"
     CDS             complement(<1..1813)
                     /locus_tag="VEO00_10350"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HYU58434.1"
                     /translation="MSWRAKLVIWAIVLGVFVLVFMPLWLPLLPDRWFADRYYVFPRV
                     EIDATVHQDGSMSVLERRTFDFQKGDFSFAFREFRPDYPGQIIDFSVSDDVHGPYTGD
                     DLVIEEFAGTYKATWYYSAHDEQRTFDIRYTARCAVGAFADGAFLYWQFIPDGFDKPT
                     EAARITVHLPGHAAEERSPGPCTAGNEPNAGRTAPETPLADGETRAWGHGPLQGNVRV
                     IDPQTVELTIRDLEPNRFVEGSVLFPADAVPFEGIALSANGPDPDDRIVQSAAEVLAQ
                     ERALAAEANATRSHVRAWDRGWKLAALLYPLLLALLVLVARWRDRVPGVPKTLPDPPE
                     EIHPVDLAVLWEGYERGSRLDDAYRAQLLSLAQKGTIEVVAEGRVSDADDLSIRKRQR
                     PEPGLDERFTEFLFDGSGDGPVKLSWLKASGVRARRAKEMREWARPVWKSLAKMADLR
                     SLRFEGVTSFLAMLAMIVVGITAAVVVHRGLAAWVGIVEGLVLFAVSRSFMPTQIEDP
                     VHHERIARWEAFRRYLLRFHTLTDAPALAVTIWERYLVYATALGIADRVVAQIQALVP
                     AELLPSPFRGAPPGPLGLSWVRTFSTAPSAAGYASIAS"
     gene            1857..2141
                     /locus_tag="VEO00_10355"
     CDS             1857..2141
                     /locus_tag="VEO00_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HYU58435.1"
                     /translation="MAVITLRAPLKDRAGGIARHDLPGATVGAALSELETRYPTVKGW
                     ILDERGRVRSHVGVFVNGERAVADTQVGPADVVHVLPSISGGSETGWVIR"
     gene            2138..3211
                     /locus_tag="VEO00_10360"
     CDS             2138..3211
                     /locus_tag="VEO00_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HYU58436.1"
                     /translation="MTELLVGTKKGLFVLRGDPASGTPFEVVTRAFPGDVVEFAVRDP
                     RSGRTFASVTSGFYGPRLFFTDGDPAGEWTQADGPVMPDGTDATIDRTWVVVPGEADG
                     QLWAGVAPAALFESRDSGTTWALNEALWSERVAGDWQPGAGGLALHSICPWPEEPERL
                     AVAVSAAGAWITEDGGRSWRAGFAGLDPHYVPEEMRPTTNVLCVHNMHRAPTRPERLF
                     LQFHGSVYRSDDEGGSWNDVAAGLPSGFGFPLAIDPADPDSAFVIPLNADTDRVTPDG
                     RVRVYETRDAGATWTARTEGLPDRDAYLTVYRQAFATAGEGDGLNLYFGATSGDVFGS
                     SDAGATWFSVRERLAPVNSVRVG"
     gene            complement(3240..>3692)
                     /locus_tag="VEO00_10365"
     CDS             complement(3240..>3692)
                     /locus_tag="VEO00_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HYU58437.1"
                     /translation="RAIGLLLVATALVAFEAELLVGALEPALDTLGLSKFFVGLIVVP
                     IVGNAAEHSSAVAFAIRDKLDVSLEIAIGSSTQVALFVAPLLVFISLLVGHPMDFVFS
                     PFEVAAVGLSTLIVALICQDGRSNWLEGTQLIGAYVIVAISFFFVKGL"
BASE COUNT          568 a         1333 c         1303 g          488 t
ORIGIN      
        1 gcgaggcgat cgacgcgtag cccgcggccg acggcgcggt cgagaaggtc cggacccagg
       61 agaggccgag gggtcccggc ggcgcaccgc ggaacggcga cggcagcagc tcggccggca
      121 ccagtgcctg aatctgcgcc accacccggt cggcgatgcc gagcgccgtg gcgtacacca
      181 ggtagcgctc ccagatggtc accgcgagcg ccggggcgtc ggtcagggtg tgaaaccgca
      241 gaaggtagcg ccggaaggcc tcccaccgcg cgatccgctc gtggtgcacg gggtcctcga
      301 tctgcgtggg catgaacgac cgtgagaccg cgaacaggac gagtccctcg acgatcccga
      361 cccacgcggc cagccctcga tgcacgacga ccgcggccgt gatccccacg acgatcatcg
      421 ccagcatcgc caggaacgag gtcacgccct cgaaccgcag gctgcgcagg tccgccatct
      481 tcgccaggct cttccacacc ggccgggccc actcgcgcat ctccttcgcc cgcctcgccc
      541 gcacgccgct cgccttcaac cacgagagct tcaccggccc gtcgcccgag ccgtcgaaca
      601 ggaactcggt gaagcgctcg tcgaggccgg gctccggccg ctggcgcttg cggatcgaca
      661 ggtcgtcggc gtccgagacg cgcccctcgg ccaccacctc gatcgtgccc ttctgggcca
      721 gcgacagcag ctgggcacgg taggcatcgt ccagtcgcga gccgcgctca tagccctccc
      781 acagcaccgc gaggtcgacc gggtggatct cctccggcgg gtcgggcagc gtcttcggca
      841 cgcccggcac gcggtcgcgc caccgggcga ccagcacgag cagggccagc agcaacgggt
      901 agagcaacgc ggcgagcttc cagccccggt cccaggcccg cacgtggctg cgcgtcgcgt
      961 tggcctccgc ggcgagcgcc cgctcctggg ccaggacctc cgcggcgctc tgcacgatcc
     1021 ggtcgtccgg gtcgggcccg ttggccgaga gcgcgatccc ctcgaagggg acggcgtccg
     1081 ccgggaagag cacgctcccc tcgacgaagc ggttcggctc gaggtcacgg atcgtcagct
     1141 cgacggtctg cgggtcgatg actcggacgt tgccctgcag cgggccgtgg ccccaggcgc
     1201 gcgtctcgcc gtcggcgagc ggcgtctcgg gagccgtgcg cccggcattc ggctcgttcc
     1261 ccgccgtgca gggtcccggc gaacgctcct ccgccgcgtg tcccggcagg tggacggtga
     1321 tccttgcggc ctccgtcggc ttgtcgaacc cgtccgggat gaactgccag tacaggaacg
     1381 cgccgtcggc gaacgcgccc accgcgcagc gggccgtgta gcggatgtca aacgtgcgct
     1441 gctcgtcgtg cgccgagtag taccaggtcg ccttgtacgt gccggcgaac tcctcgatca
     1501 ccaggtcgtc gcccgtgtag ggcccgtgca cgtcgtcgga cacggagaag tcgatgatct
     1561 gtccggggta gtccggcctg aactcccgga acgcgaacga gaagtcgccc ttctggaagt
     1621 cgaacgtccg ccgctcgagg acggacatcg agccgtcctg gtgcacggtg gcgtcgatct
     1681 cgacgcgcgg gaacacgtag tacctgtccg cgaaccaccg gtcgggcagc agcggcagcc
     1741 acagcggcat gaacacgagc acgaagacgc cgagcacgat cgcccagatc acgagcttgg
     1801 cccgccagga catcgggcag agtgtaggtc cgccgtggca cggtaggctc cgagccatgg
     1861 cggtgatcac cctccgcgcg ccactcaagg accgggccgg cgggatcgcc cggcacgacc
     1921 ttcccggtgc gacggtcggc gcggcgctct ccgagctcga gacgcgctac ccgaccgtga
     1981 agggctggat cctggacgag cgcggacggg tccgctcgca cgtcggcgtg ttcgtcaacg
     2041 gggagcgggc ggtggccgac acgcaggtcg ggcccgcgga cgtggtgcac gtcctgccgt
     2101 cgatctcggg agggagcgaa acgggatggg tgattcgatg accgagctgc tcgtcggcac
     2161 gaagaagggc ctgttcgtcc tgcggggcga tcccgcgtcc ggaaccccgt tcgaggtggt
     2221 cacccgcgcc ttccccggcg acgtagtcga gttcgcggta cgcgatccac ggtcgggccg
     2281 cacgttcgcc agcgtgacct ccgggttcta cggcccgcgg ctgttcttca ccgacggcga
     2341 ccccgccggc gagtggacgc aggccgacgg gccggtcatg cccgacggta ccgacgccac
     2401 catcgaccgc acctgggtgg tcgtcccggg cgaggccgac gggcagctct gggcgggcgt
     2461 ggcgcccgcc gcgctgttcg agagccggga ttccggcacg acgtgggcgc tgaacgaggc
     2521 actgtggagc gaacgtgtgg ccggcgactg gcagcccggg gccggtggcc tggcgctgca
     2581 ctcgatctgc ccgtggccgg aggaaccgga gcgcctggcg gtggccgtca gcgccgccgg
     2641 cgcctggatc accgaggacg gcggccggtc gtggcgcgcg ggctttgccg gcctcgaccc
     2701 gcactacgtc cccgaggaga tgcgcccgac cacgaacgtg ctctgcgtcc acaacatgca
     2761 ccgggcgccg actcggccgg agcgcctgtt cctgcagttt cacggcagcg tgtaccgcag
     2821 cgacgacgag ggcggctcct ggaacgacgt cgccgcagga ctgccctccg ggttcgggtt
     2881 cccgctggcg atcgacccgg ccgaccccga cagcgcgttc gtcatccccc tgaacgccga
     2941 cacggaccgc gtcactcccg acggcagggt ccgcgtgtac gagacccgcg atgccggcgc
     3001 cacgtggacg gcgcggaccg agggactgcc ggatcgcgac gcgtacctca ccgtctaccg
     3061 gcaagcgttc gctacggccg gcgagggtga cgggctgaac ctctacttcg gcgcgacgag
     3121 cggcgacgtg ttcggctcgt ccgacgccgg ggcgacctgg ttctcggtcc gggagcggct
     3181 cgcaccggtc aacagcgtgc gggtcgggta ggacccgccc gccgggtcga cgcggccggc
     3241 tatagaccct tcacgaagaa gaacgagatc gccacgatca cgtaggcgcc gatcagctgc
     3301 gtcccctcca gccagttcga gcggccgtcc tggcagatca gcgccacgat cagggtggag
     3361 aggccgaccg ccgccacctc gaacgggctg aagacgaagt ccatcgggtg gcccaccagc
     3421 aggctgatga acaccagcag cggcgcgacg aacagcgcca cctgtgtcga ggacccgatc
     3481 gcgatctcga gcgagacgtc gagcttgtcg cgtatcgcga aggccaccgc ggaggagtgc
     3541 tcggccgcgt tgccgacgat cggcacgacg atcagcccga cgaagaactt cgacagcccg
     3601 agcgtgtcga gcgccggctc cagcgcgccg accagcagct ccgcctcgaa cgccaccagc
     3661 gcggtggcga cgagcagcag acctatcgcc ct
//
LOCUS       DASPWO010000456         3037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1166819, whole genome shotgun sequence.
ACCESSION   DASPWO010000456 DASPWO010000000
VERSION     DASPWO010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166819"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="VEO00_10370"
     CDS             <1..1657
                     /locus_tag="VEO00_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58438.1"
                     /translation="LLAGRDQSAVYPAKVEVRSADVALAVLRAPILFVAEHQDVPLRV
                     TTTFALDRPWSLDPSGAFVGPAMERAVDEERPLQGLVAALGRIDPLVVNLAVSPLLVE
                     QLGRMADGYTVREGTTLRSVASDEPAARGAARMLDALAELARRPGTELLSFPYAAPSI
                     PALAAGILAADLPLQLVRGNRELERALGTAPNDQLIRPPRGLVDRSSLAALSQAGVSA
                     VLLDAGRAVAPPRDLGFSPVSTGVVTAGSAELDGILPSADATALLGRARDDPVLGAQV
                     LLGELAQIYFEEPDQLRGIALLFGESELPDPDFAEAFLEGLADAPLLLPVRASELLDE
                     LPPVEDLPLVPRPGSRFSGLYESRIGLARRDIASLRAVIDAPIALPGRLETRVLLAEG
                     AQFLHSEASGGVLLGSVTSRVSTELAKIEPPPEGFTVTLASRGGQVPVTIHNGTGYPV
                     RVRVTLRSNGLVFQEGGSQERRLTGETDTLRFQATARRGGRFSVDVILTTPDGSGRLS
                     TSRIVVRSTVYNRVALILTIGAALFLLVWWGRGVLARRRAASG"
     gene            1777..>3037
                     /gene="murJ"
                     /locus_tag="VEO00_10375"
     CDS             1777..>3037
                     /gene="murJ"
                     /locus_tag="VEO00_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HYU58439.1"
                     /translation="MSIGTALSRLTGFLRVAMLAFALGVQESRLADAYNVANTTPNMI
                     YDLVLGGIVASVFVPVFVEWLETRGGEATREAARAVLSISVIVLGAIALAGILGARLI
                     VGLYTARFEGPQSEAARALATFFLRWFMPQIVLYGIGAGVLTALLNAHRRFAAPMFAP
                     ILNNLLVTGTFFLFASLPGPRTPAPSTITDLQRYVLVLGTTLGVVGMTVALWPSLRRI
                     GFRWRWRLDLGDPGFRRIGRLAGWVFLYVAVNQIGYLVVIVLAAQVQGGYTAYTSAFI
                     FFQLPHAIFAVSIMTALLPSLSSRWANRDPAEFRSLLSQGIRATAFIVVPAAFGYIAL
                     ATPIVRLLLQHGVTRSQSTELLSHVLVMFSVGLFSFSSFQLLLRAFYAMQNTKTPAMI
                     NVAAVAINVVVDVVLFRYLRVEGLALGH"
BASE COUNT          349 a         1143 c         1046 g          499 t
ORIGIN      
        1 cctgctcgcc ggccgcgacc agagcgccgt ctacccggcg aaggtcgagg tgcgctcggc
       61 ggacgtcgcg ctcgcggtgc tgcgcgcgcc gatcctcttc gtcgccgagc accaggacgt
      121 gccgcttcgg gtcaccacga cgttcgccct cgaccgtccc tggtccctcg atccctccgg
      181 cgccttcgtc ggaccggcga tggagcgcgc cgtcgacgag gagcggccgc tgcagggact
      241 cgtcgcggcg ctcggccgca tcgacccgct cgtggtcaac ctggccgtct ctccgctgct
      301 cgtcgagcag ctcgggcgga tggcggacgg gtacacggtg cgcgagggca cgacgctgcg
      361 gtcggtggcg agtgacgagc cagccgcacg gggagcggcg cggatgctcg acgccctcgc
      421 ggagctggcc cgccggccgg ggaccgagct gttgtccttc ccctacgccg ccccgagcat
      481 cccggccctc gccgcgggga tcctcgccgc cgacctgccc ctgcagctcg ttcgtggcaa
      541 ccgcgagctg gagcgggctc tcggcacggc gcccaacgac cagctgatcc ggcccccccg
      601 cggcctggtg gaccgctcgt cgctcgccgc gctgtcgcag gccggggtct cggcggtgct
      661 gctcgacgcc gggcgcgccg tggccccgcc ccgcgacctg ggcttcagcc ccgtgtccac
      721 gggcgtggtc accgccggga gcgccgagct ggacgggatc ctgccgagcg cggacgccac
      781 cgccctgctg gggcgggcac gcgacgaccc cgtgctcggc gcgcaggtgc tcctcgggga
      841 gctggcgcag atctatttcg aggagcccga tcagcttcgc gggatcgcgc tcctgttcgg
      901 cgagtccgag ctccccgacc cggacttcgc cgaggcgttc ctcgaggggc tggccgacgc
      961 tccgctcctg ctccccgtgc gcgcctccga gctgctcgac gagctgccgc ccgtggagga
     1021 tctcccgctc gtcccccgcc ccgggtcacg gttctccggc ctgtacgaga gccggatcgg
     1081 cctggcgcgc cgtgacatcg ccagcctgcg cgcggtgatc gacgcgccga tcgccctccc
     1141 gggccggctc gagacgcggg tcctgctggc ggagggggcg cagttcctgc acagcgaggc
     1201 gagcgggggc gtcctcctcg gctcggtcac gtcgcgggtg agcacggagc tggcgaagat
     1261 cgagccgccg ccggagggct tcacggtcac cctcgcctcg cgaggcgggc aggtcccggt
     1321 gacgatccac aacggcaccg ggtatccggt ccgcgtccgg gtgaccctgc gctcgaacgg
     1381 gctcgtgttc caggaaggcg gcagccagga acggcggctg accggcgaga ccgacaccct
     1441 gcgcttccag gccacggccc gacgcggcgg gcgcttctcc gtcgacgtca tcctgacgac
     1501 gcccgacggc tcagggcggc tctccacctc gcgcatcgtc gtccgctcga ccgtctacaa
     1561 ccgcgtcgcg ctgatcctga cgatcggggc ggccctgttc ctcctggtgt ggtggggccg
     1621 cggcgtcctc gcccggcgcc gggcggcctc cggttgagcg cccccgacga cacgtcctct
     1681 ttggacccca ccccaccgtc ttcaccgggc ggtcccatcc ccgcgggccg accggccgag
     1741 gcctcggaac ggcggctgat ccaccacacg gcggtcatgt cgatcgggac cgcgctctcg
     1801 cggctgaccg ggttcctgcg ggtggcgatg ctcgccttcg cgctcggcgt ccaggagagc
     1861 cggctggccg acgcctacaa cgtggcgaac accacgccga acatgatcta cgacctcgtc
     1921 ctcggcggga tcgtcgcctc ggtcttcgtg ccggtgttcg tcgagtggct ggagacgcgg
     1981 ggaggggaag cgacgcgaga agctgcccgg gcggtcctgt cgatctccgt gatcgtgctc
     2041 ggggcgatcg ccctggccgg catcctcggc gcgcggctca tcgtcggcct gtacaccgcc
     2101 cgcttcgagg gcccgcagag cgaggccgcc cgcgcgctgg ccaccttctt cctgcgctgg
     2161 ttcatgccgc agatcgtcct ctacggaatc ggcgccgggg tgctgacggc actgctgaac
     2221 gcgcaccggc gcttcgccgc gccgatgttc gccccgatcc tgaacaacct cctggtcacc
     2281 gggacgttct tcctgttcgc ctcgctgccg ggcccccgta ccccggcgcc gtcgaccatc
     2341 acggacctcc agcggtacgt gctggtgctc gggacgaccc ttggggtggt cgggatgacg
     2401 gtggccctgt ggccctcgct ccgccggatc ggattccggt ggaggtggcg gctcgacctc
     2461 ggggaccccg ggttccgccg gatcgggcgc ctggccgggt gggtcttcct gtacgtcgcc
     2521 gttaaccaga tcggctacct ggtggtgatc gtgctggccg cgcaggtgca gggcggatac
     2581 accgcctaca cgtcggcctt catcttcttc cagctcccgc acgccatctt cgcggtgtcg
     2641 atcatgacgg cgttgctccc gtcgctgtcc tcccgatggg ccaaccggga ccccgcggag
     2701 ttccgctcgc tcttgtcgca ggggatccgg gccaccgcgt tcatcgtggt ccccgcggcg
     2761 ttcggctaca tcgcgctcgc cacccccatc gtccggctgc tgcttcaaca cggcgtcacc
     2821 cggagccaga gcaccgagct cctgtcacac gtgctggtca tgttctcggt ggggctgttc
     2881 tcgttctcga gcttccagct cctcctccgg gccttctacg ccatgcagaa cacgaaaacc
     2941 ccagcgatga tcaacgtggc cgccgtggcg ataaatgttg tagtcgacgt cgttctgttc
     3001 cgatatctcc gggtggaggg tctcgcgttg ggacacg
//
LOCUS       DASPWO010000457         3747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_996630, whole genome shotgun sequence.
ACCESSION   DASPWO010000457 DASPWO010000000
VERSION     DASPWO010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996630"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VEO00_10380"
     CDS             <1..584
                     /locus_tag="VEO00_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYU58440.1"
                     /translation="HALGVRPGTTTAIYGAGAVGLSAVMAAAVAGAGQIIAVDRHRSR
                     LDLALELGATHVVHVSDAPDAAREIGQQVAKISGGGTHTALDTTGAVPVIAAGLSGLR
                     TRGVIGLISAASGDIVLPRDALAPGKTVTGILEGDAVPRVLIPRLIELWRQGRLPFDR
                     LVRTYPMRDINQAEADMASGETIKPVLIPEESA"
     gene            581..2038
                     /locus_tag="VEO00_10385"
     CDS             581..2038
                     /locus_tag="VEO00_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014925637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HYU58441.1"
                     /translation="MTAPAHVRSQLFIGGDWHSPHEPELMAEIVSPSTETPFAAAPRG
                     STADVDAAVRAARTALKAAPWAGSTGTRRAAWMRSLADELESRGDHTAVLVSGENGMP
                     ISLARVAEGKGPAGTLRYYADLAEQTPAEQRRPSARGGRATVVRREPVGVVAAVVPWN
                     FPQSLTMFKVAPALAAGCTVVIKPAPETAVDAFALADAAQACGFPPGVINVIAGGRDV
                     GAYLVAHPGINKVAFTGSTAAGRAIGEVCGRLLRPVTLELGGKSAAIVLDDADLDQTV
                     RGLSWASLLNNGQTCYLSSRVLAPRSRYAEVVDAVADLAGSLKVGDPFDPSTRVGPLV
                     TRQQRDRVEGFISAGRSEGARVVTGGGRPEHLPVGWYVEPTVLADLAPHSTPAREEIF
                     GPVLVVLPYDDIDDAIRLANDSEYGLGGTVWTRDVERGLEIARRVETGSIGVNFFDLD
                     LGAPFGGVKSSGLGRELGPEGLDTYVSLKSVYLPG"
     gene            2044..2517
                     /locus_tag="VEO00_10390"
     CDS             2044..2517
                     /locus_tag="VEO00_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58442.1"
                     /translation="MTVRVRSSPVQRADDLQGNGRRELSRTIRLPAALSKGSSTMRSY
                     QQITREMGDQWVAALKRAEDAVGSAAESIRGKIDLPQIPVPEQFNKLNAALTGRLPMP
                     GEIVQANFQLTERLLGAQRDLALRLIGATESAIEPAEQPTARKAPAAKKTASNSK"
     gene            complement(2660..3043)
                     /locus_tag="VEO00_10395"
     CDS             complement(2660..3043)
                     /locus_tag="VEO00_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58443.1"
                     /translation="MSNRSRRIARRDEATAAWFDGLGEGRLLLRACSSGHLSRPDVLA
                     CDVCSDTDLSWVESRGIGSVVSLAVDHSGAEATLLAIVELVEGPWLVTRIDGPRVERG
                     GDVVVRIVRPDEGEAYPVAAAARPN"
     gene            complement(3040..>3747)
                     /locus_tag="VEO00_10400"
     CDS             complement(3040..>3747)
                     /locus_tag="VEO00_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYU58444.1"
                     /translation="DRYMSTFGVTHDQLGSVAVSQRKWAELNPIAAMRSPLTLEDYHA
                     SRWVVEPLHLLDCCLVSNGAAAVVVTSSERARDLRQPPVHVRGWGQAHPAPPLRADST
                     FGLTTGAKASGRAALGMAGMGAADIQLREIYDCYTYTVLVTLEDYGFCGPGEGGALAA
                     SGALAPGGNLPTNTGGGQLSSFYLWGMTPLHEAVTQVRGQAGDRQVVDHDTAIVSGNG
                     GILEHHSTLVLTSEAAA"
BASE COUNT          590 a         1357 c         1273 g          527 t
ORIGIN      
        1 tccacgcgct cggggtgagg ccgggcacga cgacggcgat ctatggcgcc ggcgccgtcg
       61 ggctctcggc cgtcatggcg gccgccgtgg ccggcgccgg gcagatcatc gccgtggatc
      121 gacaccgctc ccgcctcgac ctggccctgg aactcggagc gacccacgtg gtccacgtct
      181 cagatgcgcc cgatgcggcg cgcgagatcg gccagcaggt cgcgaagatc tccggcggcg
      241 gcacgcacac cgcactggac accactggcg ccgttccagt gattgccgcc ggcctgagtg
      301 ggctgcgcac tcggggcgtg atcgggttga tctcggcggc atccggagac atcgtcctcc
      361 cgcgcgacgc gctggcccca gggaagacgg tgaccggcat cctcgaaggc gacgctgttc
      421 cccgcgtcct catcccccgg ctgatcgagc tgtggcgcca gggccgcctc ccgttcgacc
      481 gcctcgtccg cacctatccg atgcgcgaca tcaaccaggc cgaggccgac atggcgagcg
      541 gcgagaccat caagcccgtc ctgatccccg aggagtccgc atgaccgctc ccgcgcacgt
      601 tcgcagccag ttgttcatcg ggggcgactg gcactcgccc catgaacccg agctgatggc
      661 agagatcgtc tccccgtcga ccgagacgcc gttcgccgcg gcacccaggg gatcgaccgc
      721 cgatgtcgac gctgccgtga gagccgcccg gactgctctc aaggccgcac cctgggcagg
      781 atccaccggc acacggcgcg ccgcctggat gcggtcgctg gcggacgagc tcgagtcacg
      841 cggcgatcac actgccgtgc tggtgagcgg cgagaacggc atgccgatca gcctggcgcg
      901 tgtcgccgaa ggcaagggcc ccgccgggac gctgcggtac tacgccgatc tggcggagca
      961 gacgccggcc gaacagcggc gcccctccgc ccgcggtggc cgggccaccg tcgtgcgccg
     1021 tgagcccgtc ggcgtcgtcg ccgcggtggt cccgtggaac tttccgcagt ccctgaccat
     1081 gttcaaggtc gcgcccgccc tcgccgccgg gtgcacggtc gtcatcaagc ccgcgccgga
     1141 gaccgcggtc gacgcgttcg cgctggcgga cgccgcgcaa gcctgcggct tcccaccggg
     1201 ggtgatcaac gtgatcgccg gaggccgcga cgtcggcgcc tacctggtgg cgcatcccgg
     1261 gatcaacaag gtcgccttca ccggttccac cgcggccgga cgggcaatcg gcgaggtctg
     1321 tgggcgcctc ctacggcccg tcaccctcga gctcggcggc aagagcgccg caatcgtcct
     1381 cgacgacgcc gacctggacc agactgtccg cggactgtcc tgggcgagcc tgctcaacaa
     1441 cggccagacc tgctacctga gcagccgggt cctcgccccc cgaagtcgtt acgccgaggt
     1501 agtcgacgct gtcgcagatc tcgccggctc gctcaaggtc ggagacccct tcgacccgag
     1561 cacgcgcgtc ggaccacttg tgacccgaca gcagcgggac cgcgtggagg gattcatctc
     1621 ggccgggcgc agcgagggcg cgcgggtggt gacgggcggt ggccggcccg agcatctgcc
     1681 agtgggctgg tacgtcgagc ccacggtgct cgccgacctg gctccgcact ccacgcccgc
     1741 gcgcgaggag atcttcggcc ccgtgctggt ggtcctcccc tacgacgaca tcgacgacgc
     1801 aatccgcctc gcgaacgact ccgagtacgg tctcggcggc accgtgtgga ctcgggatgt
     1861 cgagcgcggt ctcgagatcg cccggcgcgt agagacgggg tcgatcggcg tgaacttctt
     1921 cgatctcgac ctcggcgcgc ccttcggtgg cgtgaagtcc tccggcctcg gccgcgagct
     1981 cggccccgag ggcctggaca catacgtgtc gctcaagtcg gtgtatctgc ccggctgaga
     2041 acggtgacgg tccgggtgcg gtcatctcca gtgcagcgcg ccgatgacct acaaggcaac
     2101 ggtcgacggg agttgagccg gaccatccgg ctaccggcgg ccttgagtaa aggaagttca
     2161 acgatgagaa gctatcagca gatcacgcgc gagatgggcg accagtgggt cgcggctctg
     2221 aagcgagccg aggacgcggt cgggtcggca gcggagagca tccgcggaaa gatcgacctg
     2281 ccgcagatac cggttcccga gcagttcaac aagctcaacg cggccttgac cggacgcctc
     2341 cccatgccgg gcgagatcgt tcaggccaac ttccaactga ccgagcgtct gctgggcgcc
     2401 cagcgggacc tcgccctccg cctcattggc gccaccgagt cggcaatcga gcccgccgag
     2461 cagcccaccg cgaggaaggc gccggcggcg aagaagacgg ccagcaacag caagtaggcg
     2521 cttcactcct gacgtgttca ctctccggcc ggacagcaag gccggagggt ggacacgcga
     2581 cgggagaact gagccgcccc gcgtgctccc agcgaccggg gccgggtgtc tccgactccc
     2641 tggcccaccc ggccagacgt cagttcggac gggctgcggc ggcgacggga taggcctcgc
     2701 cctcgtcggg gcgcacgatg cgtacgacca cgtccccgcc acgttcgacc cgaggaccgt
     2761 cgatgcgggt cacgagccac gggccctcga cgagttcgac gatggccagc agcgtggctt
     2821 ccgcgccgga gtggtcgacc gcgagcgaga ccaccgatcc gatgccacgg gactcgaccc
     2881 aggacaggtc ggtgtcacta cacacgtcac aggccagcac gtccggacga ctcaggtggc
     2941 ccgaggagca cgcacgaagg agcagtcgtc cctcgccgag cccgtcgaac catgcggccg
     3001 tcgcctcgtc gcgacgcgcg atccttcgac tgcggttgct catgccgctg cctccgaggt
     3061 caacaccaga gtggagtggt gctcgaggat gccgccgttc ccgctcacga tcgcggtgtc
     3121 gtggtcgacc acctggcggt cgcccgcctg gccacgcacc tgcgtgacag cctcgtgcag
     3181 cggcgtcata ccccacaggt agaacgacga gagctgacca ccgccggtgt tggtcggtag
     3241 gttcccaccc ggcgcgaggg cacctgacgc tgccagggcg ccgccttcgc cgggtccgca
     3301 gaagccgtag tcctccagcg tcaccaagac ggtgtaggtg tagcagtcgt agatctcccg
     3361 cagctggatg tcggccgcgc ccatccctgc catgcccagc gcggcccggc ccgaagcctt
     3421 tgcccccgtg gtgagaccga acgtgctgtc cgcgcgcagc ggcggtgccg gatgggcctg
     3481 cccccacccc cgcacatgga cgggcggctg acggaggtcc cgcgcgcgtt ccgagctggt
     3541 cacgaccacc gccgccgcgc cgttggagac caggcaacag tcgaggaggt gcaacggctc
     3601 cacgacccac cgcgacgcgt ggtagtcctc cagcgtcaac ggggaccgca tcgccgcgat
     3661 cgggttgagc tcggcccact tgcgctggga cacagccacc gatcccaact ggtcgtgcgt
     3721 gacgccgaac gtcgacatgt agcgatc
//
LOCUS       DASPWO010000458         3529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_324192, whole genome shotgun sequence.
ACCESSION   DASPWO010000458 DASPWO010000000
VERSION     DASPWO010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324192"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1786)
                     /locus_tag="VEO00_10405"
     CDS             complement(272..1786)
                     /locus_tag="VEO00_10405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HYU58445.1"
                     /translation="MVEFSARTRSAAIDRMTDGPLDVLVVGGGATGCGVALDAAARGF
                     RTALVERDDFASGTSGRSSRLIHGGIRYLSKGDVGVVYGSLRERQTLLRLAPHLVTPL
                     PFFVVAPRGRARVLVRAGLVMYEALAAGRGIGRHRAVPEAESGRYAPGLAHASRGYVY
                     PDCRTDDARLTLEVARAAAAKGASLANHVAAVSVGAGAGGFEVDIADSMEGEHRTVRA
                     RAVVNAGGVWAHEVHALAGPSDVRLRPSKGVHLVFRAADLPVRAATLFPSGAHDRAFV
                     FAIPWGPRVYVGTTDSPYQGSLDEPGLDDPDADYLLAAVNGAFGCSLARSDVTAWWAG
                     LRPLLARGDASTRDLSRRHVVIEDPPGFLTVTGGKLTAYRAMSQDVTNRLAKLFGSRA
                     RSTTRSIPLGFTGDLTAALGAGEYEGAEAGLAPEAGRRLVRRYGDDWGEALDRIRSDP
                     ALGEPLVEGLPVLRVEASLARDREMALTDDDVLIRRTRLATMDARATELVKGRS"
     gene            1834..2931
                     /locus_tag="VEO00_10410"
     CDS             1834..2931
                     /locus_tag="VEO00_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020118857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYU58446.1"
                     /translation="MPSARATRLRRASREAARAGLSGLIVTPSADLIYLTRYDPPPLE
                     RLTALVLRPDRDPILMVPELEKPRAEHDGVGKLAELVPWRDGQEPYARLAKLLGRRGR
                     YAVSDRMPARHLLGLQRAAPSVAFEPAAGVLAALRAVKDAAEIDFLRRAARGADEAFG
                     RIADERLEGLREEQVAARLADLLLETGHDQVAFTIVASGPNGASPHHEPGGRTIQGGE
                     AVVLDFGGRVGGYCSDISRTVAVERTPREFEDVYRVVQEAQEAAFRAVRPGVPAEEVD
                     RTARAVIERAGYGDRFVHRTGHGIGLDEHEEPYIVAGNTTPLAAGMCFSIEPGIYLDG
                     AFGVRIEDIVTVTDTGAERLNHASRDLRITA"
     gene            3032..>3529
                     /locus_tag="VEO00_10415"
     CDS             3032..>3529
                     /locus_tag="VEO00_10415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU58447.1"
                     /translation="MPLLNIPDELIELRSSLRRFIDREVRPVEDEHRQEILERATWEG
                     IREDRMKVRKRSAELGFYTLHMPEEVGGGGLSLLGQVMLHEEIYTGGLILAGGESILP
                     IVTGPTPIYLDVTEHQREKYLDPLMRMDKTTCFALTEPGAGSDAIRIQTRAEKSDGGW
                     ILNGRK"
BASE COUNT          506 a         1315 c         1284 g          424 t
ORIGIN      
        1 cgaagatcta ccgcggagag cacgtcccgc acccgcacat caaggagttc gtcggcgccc
       61 gggtggaggt cgacgccgac cgcccgctgc agatcgaggc cgacggcgag gtcctcggca
      121 ccacccccgc cgtcttcgag atcgtcccgg aggcggtccg gctgaagatc tgatgcgccg
      181 gggctccgcc cccaccgatc gaccgcaacg acgagctcca ccaccccggc ttcccctggg
      241 tgacctgagc gcgcgcgccg gtcaggccgg cctacgaacg gcccttgacc agttccgtgg
      301 cgcgggcgtc catcgtggcc aggcgggtgc ggcggatcag gacgtcgtcg tcggtgaggg
      361 ccatctcgcg gtcgcgggcc agtgaggcct cgacgcgcag cacgggcagc ccctcgacga
      421 gcggctcgcc gagggcgggg tcggagcgga tccggtcgag ggcttccccc cagtcgtcgc
      481 cgtagcggcg caccagccgc cgcccggcct ccggcgccag cccggcctcg gcgccctcgt
      541 actccccggc gcccagggcg gcggtcaggt ctcccgtgaa ccccaacggg atggaccgtg
      601 tggtcgagcg agcgcgcgag ccgaagagct tcgccaagcg attcgtcacg tcctgggaca
      661 tggcgcggta ggcggtgagc ttcccgccgg tcacggtcag gaaccctggc gggtcctcga
      721 tcaccacgtg ccggcgcgag aggtcgcgag tggaggcgtc cccgcgcgcc agcagcggcc
      781 gcagcccggc ccaccaggcc gtgacgtccg agcgcgccag cgagcacccg aacgcgccgt
      841 tcaccgcggc cagcagatag tcggcgtcgg ggtcgtcgag cccgggctcg tccagcgagc
      901 cctgataagg actgtcggtc gtcccgacgt acacgcgcgg gccccacggg atggcgaaga
      961 cgaaggcgcg atcgtgcgcg cccgacggga agagcgtggc ggcgcggacg ggcaggtcgg
     1021 cggcccggaa caccaggtgg acgcccttgc tcggccgcag gcggacgtcg gacggtcccg
     1081 ccagcgcgtg cacctcgtgg gcccagaccc cgccggcgtt gaccaccgcc cgcgcccgga
     1141 cggtccggtg ctcgccctcc atgctgtccg cgatgtcgac ctcgaacccg ccggcgccgg
     1201 ccccgacgga taccgccgcc acgtggtttg cgagggaggc ccccttcgcg gcggccgccc
     1261 gggcgacctc gagcgtcagc cgggcgtcgt cggtgcggca gtccgggtac acgtagccgc
     1321 gcgaggcatg ggccaggccc ggcgcgtacc gtcccgattc cgcctccggc accgcccggt
     1381 gccgcccgat gccccgcccg gccgccagcg cctcgtacat gacgagcccc gcccggacca
     1441 gcacgcgggc gcggcctcgc ggcgcgacga cgaagaacgg gagcggcgtc accaggtggg
     1501 gagccagccg gagcagcgtc tgccgctcgc gcaggctccc gtagaccacg ccgacgtcgc
     1561 ccttggagag gtagcggatc ccgccgtgga tcaggcggga ggaccgccca gaggtcccgc
     1621 tggcgaagtc gtcgcgctcg acgagcgcgg tccggaagcc gcgggcggcg gcgtcgagcg
     1681 ccaccccgca gccggtcgcg ccacccccga ccaccaggac gtcgagcggg ccgtcggtca
     1741 tccgatcgat ggcggcggag cgggtgcggg ccgagaactc gaccacgtcg gcgatgctac
     1801 cggcccgtcc ggactggcgt ttatcatccc cggatgccat ccgccagggc aacgcgcttg
     1861 cggcgcgcca gccgggaggc cgcacgggcg ggcctctcgg gtctgatcgt cacgccctcc
     1921 gccgacctga tctacctcac gcgctacgac ccaccaccgc tcgagcggct gaccgccctg
     1981 gtgttgcgcc ccgaccgcga cccgatcctg atggtccccg aactcgagaa gccccgcgcc
     2041 gagcacgacg gggtcgggaa gctcgccgag ctcgtcccct ggcgcgacgg ccaggagccc
     2101 tacgcgcgcc tggcgaagct gctcggacgg cgcggccgct acgcggtgtc cgaccggatg
     2161 ccggcccgcc acctgctcgg cctgcagcgc gcggccccgt cggtggcttt cgagcccgcg
     2221 gccggcgtcc tcgctgcgct ccgggccgtg aaggacgcgg cggagatcga tttcctgcgc
     2281 cgggccgcgc gcggcgccga cgaggccttc gggcggatcg ccgacgagcg cctggaagga
     2341 ctgcgcgagg agcaggtggc tgcccgcttg gccgacctgc tactcgagac cggccacgac
     2401 caggtggcgt tcaccatcgt cgcctccggc ccgaacggcg cctcgccgca ccacgagccc
     2461 ggcgggcgga cgatccaggg cggggaggcg gtggtactcg acttcggcgg gcgggtcggt
     2521 ggctactgct cggacatcag ccgcacggtg gccgtcgagc gcaccccgcg cgagttcgag
     2581 gatgtctacc gcgtggtcca ggaggcgcag gaggccgcgt tccgcgccgt ccgcccaggc
     2641 gtcccggcgg aggaggtgga ccgcaccgcg cgagcggtca tcgagcgcgc cggctacggc
     2701 gaccggttcg tccaccggac cggccacggc atcggcctgg acgagcacga ggagccctac
     2761 atcgtggccg gcaacaccac cccgctcgcc gccggcatgt gcttctcgat cgagccgggc
     2821 atctacctcg acggcgcctt cggcgtccgc atcgaggaca tcgtcacggt caccgacacc
     2881 ggcgcggaac gcctgaacca cgcctcacgg gacctccgca tcaccgcgtg acgacgcacg
     2941 gcgcggcagg aatccggtag gctaggggta acctacggag cggtaggtta gtgcccggtt
     3001 ccggtgaacg gcgagcgaag gaggcccggt cgtgcccctg ctgaacatcc ccgacgagct
     3061 gatcgagctg cggtcgtcgc tgcggcggtt catcgaccgc gaggtgcgcc ccgtcgagga
     3121 cgagcaccgc caggagatcc tggagcgcgc cacctgggag gggatccggg aggaccggat
     3181 gaaggtccgc aagcgctcgg ccgaactcgg gttctacacc ctgcacatgc ccgaggaggt
     3241 cggtgggggc ggcctgtcgc tgctcggcca ggtgatgctg cacgaggaga tctacacggg
     3301 cggcctgatc ctcgcgggcg gcgaatcgat cctgccgatc gtcaccgggc cgacgccgat
     3361 ctacctcgac gtcaccgagc accagcgcga gaagtacctc gacccgctga tgcggatgga
     3421 caagaccacg tgcttcgcgc tgaccgagcc gggggccggg tccgacgcca tccggatcca
     3481 gaccagggcg gagaagtccg acggcgggtg gatcctgaac ggccgcaag
//
LOCUS       DASPWO010000459         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_488716, whole genome shotgun sequence.
ACCESSION   DASPWO010000459 DASPWO010000000
VERSION     DASPWO010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488716"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VEO00_10420"
     CDS             <1..825
                     /locus_tag="VEO00_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HYU58448.1"
                     /translation="LATDTTADRLGFDRAFDNSIDAVSDRDFALELLAASAIAAVHVS
                     RLAEDVVLWCTEEFGFARPAEADATGSSMMPQKRNPDVAELARGKAGRVIGDLMAVAV
                     AMKGLPLAYNRDLQEDKEPLFDAHDALAGALTAMAAMVRGLAFDIARMRAAVGGGLFA
                     TDLAEALVAGGTPSREAHRAVGELVRRLEAEGRTTADVRPDEWPALLPGLPPEASAAA
                     TPSPEAAVARRTTPGGPSPPSVAGQLTTLRGRLQAVRERAAAVRPYTAAGTVADDR"
     gene            858..2540
                     /gene="ilvD"
                     /locus_tag="VEO00_10425"
     CDS             858..2540
                     /gene="ilvD"
                     /locus_tag="VEO00_10425"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HYU58449.1"
                     /translation="MTESIDPRHRSRDVTDGIERAPARSMLRAVGFTDEDFERPQIGV
                     ASSWNEVTPCNVHLDRLAAAAKEGVRSAGGVPVEFTTIAVSDGIAMGHEGMRASLVSR
                     EVIADSVELVIHAERFDGAVTIAGCDKSLPGMVMAAVRLNLPTVFLYGGTILPGHHRG
                     RDVTVQDMFEAVGAVSAGAMSEEELRALELAACPGPGSCAGMYTANTMACAVEALGMS
                     LPGSASPPAVDPRRDELARASGVAAVEALRAGIRPREIVTREALENAIAVVMACGGST
                     NAVLHLLAIAAEAGVPLELDDFDRISRRVPHIGDVRPAGRFVMADLDRVGGLPVIMRP
                     LLDAGLLHGDALTVNGRTVAENLAALDPPAPDGDVVRPLDAPIHPHGGLAVLKGSLAP
                     DGAVTKIVGMEGHAFRGLARVFDSEEEAFDAVTTRRIGPGDVIVIRYEGPAGGPGMRE
                     MLAVTAAVVGAGHGRDVALVTDGRFSGASHGFAVGHVAPEAAVGGPIALVEEGDTIVL
                     DVDARRLDLEVSPEELGRRRDGWKPREPRYAHGALAKYARLVSSAARGAVCE"
     gene            complement(2575..>3011)
                     /locus_tag="VEO00_10430"
     CDS             complement(2575..>3011)
                     /locus_tag="VEO00_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58450.1"
                     /translation="PTVTRIPVGGEVTFTSADDMAHTVTGLGFSWGTSGDLKRGDSAS
                     FAFPDEGIFPYACMYHPGMVGAVVVGDGIGTSAAAAPVVANPSPPAPARDVDAAPAAD
                     TADIAGWWPVLAVAIAAGGLLAGFVAGRRVRALRGRERTAAA"
BASE COUNT          363 a         1123 c         1142 g          383 t
ORIGIN      
        1 ctcgccaccg acacgaccgc ggaccggctg ggcttcgacc gggcgttcga caactcgatc
       61 gacgccgtct cggaccgtga cttcgctctg gagctgctgg ccgcctccgc gatcgccgcg
      121 gtgcacgtct cgcgcctggc ggaggacgtc gtgctctggt gcaccgagga gttcgggttc
      181 gcccggccgg ccgaggccga cgccaccggc tcgagcatga tgccacagaa gcgcaacccg
      241 gacgtcgcgg agctggcgcg cgggaaggcc ggccgggtga tcggcgacct gatggcggtg
      301 gcggtggcga tgaagggact gccgctcgct tacaaccggg acctgcagga ggacaaggag
      361 ccgctgttcg acgcgcacga cgccctcgcc ggcgcgctga ccgcgatggc ggcgatggtc
      421 cgtgggctgg ccttcgacat cgccaggatg cgcgcggcgg tcggcggggg gctcttcgcc
      481 acggacctcg ccgaggcgct ggtcgccggc ggcacgccgt cccgcgaggc gcaccgggcc
      541 gtgggcgagc tggtgcgccg cctggaggcg gagggccgga cgaccgccga tgtccggccg
      601 gacgaatggc cggcgctcct ccccggcctc ccgcccgagg cctcggccgc ggccacgccg
      661 tcgccggagg cggccgtcgc tcgccggacc acgcccggcg gcccgtcgcc gccgtcggtc
      721 gccggccagc tcaccacgtt gcgggggcga ctccaggcgg tccgcgagcg ggcggcggcc
      781 gtccgcccgt acactgccgc cggtaccgtc gccgacgacc gctgaccggc ccgcccccgc
      841 tcctcggagg catcgtcatg accgaatcga tcgacccccg ccaccgcagc cgcgacgtca
      901 ccgacggcat cgaacgcgct ccggcccgct cgatgctccg cgcggtgggg ttcaccgacg
      961 aggacttcga gcgtccccag atcggcgtgg cctcgtcctg gaacgaggtc acgccgtgca
     1021 acgtccacct ggatcgcctg gccgcggcgg cgaaggaggg cgtgcgctcg gccgggggcg
     1081 tcccggtgga gttcacgacg atcgcggtct ccgacggcat cgccatggga cacgagggga
     1141 tgcgcgcctc gctcgtcagc cgggaggtga tcgccgactc cgtcgagctg gtgatccacg
     1201 ccgagcgctt cgacggcgca gtgaccatcg ccggctgcga caagagcctg ccgggcatgg
     1261 tgatggccgc ggtccggctg aacctgccga cggtcttcct ctacggcgga acgatcctcc
     1321 ccggccatca ccgcggccgg gacgtcacgg tccaggacat gttcgaggcg gtcggcgcgg
     1381 tctcggccgg cgcgatgtcc gaggaggagc tccgcgcgct ggagctggcg gcgtgcccgg
     1441 ggccgggatc gtgcgccggg atgtacaccg cgaacacgat ggcctgtgcg gtggaggcgc
     1501 tcggcatgtc gctccccggc agcgcttcgc cgcccgcggt ggacccgcgc cgggacgagc
     1561 tcgcccgggc cagtggcgtc gccgccgtcg aggcgctgcg ggccgggata cgtccgcgcg
     1621 agatcgtcac ccgcgaggcc ctggagaacg ccatcgcggt ggtgatggcc tgcggcgggt
     1681 cgacgaacgc cgtcctgcac ctgctggcga tcgccgccga ggccggcgtg ccgctcgagc
     1741 tcgacgactt cgaccggatc agccggcggg tgccgcatat cggcgacgtc cgtcccgctg
     1801 gccggttcgt gatggcggac ctcgatcgcg ttggcggcct gccggtgatc atgcggccgt
     1861 tgctcgacgc cggcctgctg cacggagacg cgctgacggt caacggccgg accgtcgctg
     1921 aaaacctcgc ggcgctcgac cctccggcgc ccgacggcga cgtggtgcgc ccgctcgacg
     1981 ccccgatcca tccccacggc ggcctggccg tcctgaaggg gtcgctggcg cccgacgggg
     2041 cggtgacgaa gatcgtcggc atggaggggc acgcgttccg cggcctggcc cgcgtcttcg
     2101 actccgagga ggaggcgttc gacgccgtca ccacgaggcg gatcgggccc ggcgacgtca
     2161 tcgtcatccg ctacgagggc ccggccggcg ggccggggat gcgcgagatg ctcgccgtga
     2221 ccgcggcggt ggtcggcgcc ggccacggcc gtgacgtggc cctcgtcacc gacggacggt
     2281 tctccggcgc ctcgcacggg ttcgcggtcg gccacgtggc ccccgaagcg gccgtcggcg
     2341 ggccgatcgc gctcgtcgag gagggcgaca cgatcgtcct cgacgttgac gcccgccggc
     2401 tggacctcga ggtctcgccc gaggagctcg gccgccggcg cgacggctgg aagccccgcg
     2461 agccacggta tgcacacggg gcgctggcga agtacgcccg cctcgtgtcg tccgcggccc
     2521 gcggggccgt ctgcgagtag ctctccggcc gggcggcccg ggatcgccgc gggttcaggc
     2581 ggcggccgtc cgctcccggc cgcgaagggc gcggacccgc cgtccggcga cgaacccggc
     2641 cagcagtccg ccggccgcga tcgccacggc cagcaccggc caccagccgg cgatgtccgc
     2701 cgtgtccgcc gcgggcgcgg cgtccacgtc cctcgccggc gcgggcgggg acgggttcgc
     2761 cacgaccggc gcggccgccg cggatgtccc gatgccgtcg ccgaccacga ccgcgccgac
     2821 catgcccggg tggtacatgc aggcgtacgg gaagatgccc tcgtcgggga acgcgaacga
     2881 ggcgctgtcg ccgcgcttga ggtccccgct ggtgccccag gagaagccga gcccggtcac
     2941 ggtgtgcgcc atgtcgtccg ccgacgtgaa cgtgacctcg ccgccgaccg ggatccgcgt
     3001 cactgttggc g
//
LOCUS       DASPWO010000460         5711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_488737, whole genome shotgun sequence.
ACCESSION   DASPWO010000460 DASPWO010000000
VERSION     DASPWO010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488737"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="VEO00_10435"
     CDS             <1..142
                     /locus_tag="VEO00_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HYU58451.1"
                     /translation="EIDREDWGLRWNVVLEGGGVLVSRKVQLELEVSAILQEDEPASQ
                     AD"
     gene            complement(139..774)
                     /locus_tag="VEO00_10440"
     CDS             complement(139..774)
                     /locus_tag="VEO00_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018822466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HYU58452.1"
                     /translation="MGGVALLEWLDPKRLIEAFGLIGIGALVFAESGVLLGLFLPGDS
                     LLFTAGILAAADTLPLLVLLPLVFVAAAAGDSVGYAFGRRAGPALYRRPDSRVFKQEH
                     LQHAREFYERRGGSTILLARFIPIVRTLAPVVAGIAGMRYRRFVGFNLIGAALWGIGV
                     TLLGYALGSVIPDIDRYLLPVIAVIVLISVTPVIREVRKRRTSGSSAAPRN"
     gene            complement(833..1384)
                     /locus_tag="VEO00_10445"
     CDS             complement(833..1384)
                     /locus_tag="VEO00_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HYU58453.1"
                     /translation="MDIQRARAIARERTEKETTYRHLVSVEGVMRALARRLGEDVELW
                     ARTGLFHDLDQDQTGGGDDVEQHARLAAEWLRAEGEDERVINGVLAHAYKEYRTDAMS
                     RAVVHADAVAGLLVAAALVRPEKATGMKVSSVKKKLKEKAFAPGVNRDEVYGAEEALG
                     MPLDDFIATGIEGLQLVAPDIGL"
     gene            complement(1390..1983)
                     /locus_tag="VEO00_10450"
     CDS             complement(1390..1983)
                     /locus_tag="VEO00_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58454.1"
                     /translation="MATRMAEAEPAGAEARLQELEARAHRRVQRAHAIAELDALFRAG
                     SAPDPLPIGFQPGRLLTMSVTPAIDALVRRIADAWMPWKGKAFDPSTMTGINIMTADA
                     RAPLRLLFGERPEPGPDGRMEVFPFRNRIAPGAVDPAVTTLKIDYDFPANVDFIRRIL
                     DELVQIGDGVYLGKILFRTRSGFHPIGFFSLQNPTQP"
     gene            complement(2057..4357)
                     /locus_tag="VEO00_10455"
     CDS             complement(2057..4357)
                     /locus_tag="VEO00_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HYU58455.1"
                     /translation="MATRLEERTRVGVGGVIKRILVGRAKPSHHLEHTLLPKFLALPV
                     FSSDPLSSVAYATEEMMLVLALAGTAAFAFMLPIALVIAAVLAIVITSYRQTVRAYPG
                     GGGSYIVAHENLGTLPGLISASALLIDYVLTVSVSVVAGVAAITSAAPGLTHLRVEMS
                     ILFIALITLANLRGVKEAGTLFAVPTYGFVACVYVTLGVGFVNCLNGCPTAASASLPV
                     ESVQGVSLYLILRAFSSGSTALTGVEAISNGVPAFRRPQSRNAAKTLAVMGAISISMF
                     LGITFLARAFDVHVSEGLVGEKSVLAQIGATLFGSGLMFYVLQAFTALILILAANTAY
                     QDFPRLSSILARDRFMPTPFRNRGDRLVFSNGVVVLALVAAALVWAFDAELTRLIQLY
                     VVGVFTAFTLSQAGMVRRWLRTREPGWKRSAVINAIGAFATGIVLVIVTLTKFTHGAW
                     IVISAMPVMILLMLGVNRHYASWSAQLRRGRVHVGEIPANTIVLAMTDLDAAAAEALG
                     YVRSLRGLPVRYVYLETGDTPPDLEERWRRFSGGGAPLEVVPSRDHPGAAIRDFVRTI
                     ERGAEDFVTVVVPEMVPRRSILFLLALSPATRIKLRLLPERGVAVMNAPVQVVEGQPV
                     GVDARPLIPTSVTTIVLVASVHDATLRAVNYARSLQATETKAVFISLDPEEIERIEED
                     WVRAGADIPLEIVGAPFRDLSVPVLEEIRRATAAPHSLAVVVLPEFVVPKLWQRLLHN
                     NTALFIKRLLLFEPRTVLVSVPYHLD"
     gene            complement(4391..5077)
                     /locus_tag="VEO00_10460"
     CDS             complement(4391..5077)
                     /locus_tag="VEO00_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010595497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU58456.1"
                     /translation="MTRVLVVDDEPQILRALRTSLSARGHEVLVAANGETALDLVASA
                     DPDLVVLDLGLPGIDGIEATRRIRSFSTVPIIVLSVREGQADKVAALDAGADDYVTKP
                     FAMEELLARMRAVGRRAGTAEATEPVLRFGPLVVDLPKHRVTRGEEPIHLTPTEWQLL
                     EAFVTHPGKLLTHRWLLGRVWGAGYGEESHYLRVFVAALRKKLEAEPGRPRWLLTEPG
                     VGYRWAEPGG"
     gene            complement(5074..>5711)
                     /locus_tag="VEO00_10465"
     CDS             complement(5074..>5711)
                     /locus_tag="VEO00_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU58457.1"
                     /translation="SIKASVTSLQDEAVDFDRGERGELLQTILEETDRLNRMVGNLLD
                     LSRMRAGALVPAREPVAVADVIGGVLSRLRPVLEGRPVEVRVPEDLPDLPLDVVQFDQ
                     VLTNLLENAVKFSPSGTAIEVTASRFRDGVQVRVVDHGEGIPEPEREQVFEPFYRRDA
                     DRAAPGSGLGLAIARAVVSVHEGRIWAEGTPAGGTTIVLELPATRDGEEPR"
BASE COUNT          885 a         2056 c         2020 g          750 t
ORIGIN      
        1 cgagatcgac cgcgaggact ggggcctgcg ctggaacgtg gtgctcgagg gcggcggcgt
       61 gctggtgagt cgcaaggtcc agctggagct cgaggtctcg gccatcctgc aggaggacga
      121 gccggcctcg caggccgact agttccgagg ggctgcgctc gaccccgacg tgcggcgctt
      181 gcgcacctcg cggatcaccg gcgtcaccga gatcaggacg atcacggcga tcaccggcag
      241 caggtagcgg tcgatgtccg ggatcacgct cccgagcgcg tagccgagca gcgtgacccc
      301 gatgccccac agcgcggcgc cgatcaggtt gaaccccacg aaccggcggt agcgcatccc
      361 ggcgatgccg gcgacgaccg gcgccagggt gcgcacgatc gggatgaacc gtgccagcag
      421 gatcgtgctg ccgccgcggc gctcgtagaa ctcgcgggcg tgctggaggt gctcctgctt
      481 gaagacccgc gagtccggcc gccggtacag cgccggcccg gcccggcggc cgaacgcgta
      541 cccgacgctg tcgccggccg cggcggccac gaacacgagc ggcagcagga cgaggagggg
      601 cagcgtgtcc gccgccgcca ggataccggc ggtgaacagc aggctgtcgc ccggcaggaa
      661 aagcccgagc aggacccccg actcggcgaa gacgagggcg ccgatcccga tcagcccgaa
      721 cgcctcgatc agccgcttcg gatcgagcca ctcgagcaga gccaccccgc ccaccgcgcg
      781 agtgtagcgg cgcccgaagg cggttccgta cgaccgtcgg cgccagcgaa ggctacaggc
      841 cgatgtcggg ggcgacgagc tggaggccct cgatgccggt ggcgatgaag tcatcgaggg
      901 gcattccgag cgcctcctcg gcgccgtaga cctcgtcgcg gttcacgccg ggggcgaagg
      961 ccttctcctt cagcttcttc ttcacgctcg agaccttcat cccggtggcc ttttccgggc
     1021 gcaccagcgc ggcggcgacc agcaggcccg ccacggcgtc ggcgtggacc accgcccgcg
     1081 acatcgcgtc ggtgcggtac tccttgtagg cgtgggccag caccccgttg atcacccgct
     1141 cgtcctcgcc ctcggcccgg agccactccg ccgccagccg ggcgtgctgt tccacgtcgt
     1201 cgccgccgcc ggtctggtcc tggtcgaggt cgtggaacag gccggtgcgc gcccacagct
     1261 ccacgtcctc gcccaggcgg cgggcaagcg cgcgcatcac gccctcgacg ctgaccaggt
     1321 gccggtaggt ggtctccttc tccgtccgct cgcgggcgat ggcccgggcc cgctgtatgt
     1381 ccatcccgct cagggctgcg tggggttctg cagcgagaag aacccgatcg gatggaaccc
     1441 ggatcgcgtg cggaacagga tcttgccgag gtacaccccg tcgccgatct ggaccagctc
     1501 gtccaggatg cggcggatga agtcgacgtt cgccgggaag tcgtagtcga tcttcagcgt
     1561 cgtgacggcg gggtccacgg cacccggcgc gatgcggttg cggaacggga agacctccat
     1621 ccggccatcg ggacccggct ccggccgctc cccgaacagc agccgcaacg gggcgcgggc
     1681 gtccgcggtc atgatgttga tcccggtcat cgtcgaaggg tcgaacgcct tgcccttcca
     1741 cggcatccag gcatcggcga tccgccgcac cagcgcgtcg atcgctgggg tcaccgacat
     1801 cgtcagcagg cgcccgggct ggaagccgat cggcagcggg tcgggcgcgc tccccgcgcg
     1861 gaatagcgcg tcgagctcgg cgatggcgtg ggcgcgctgg acccggcgat gagcccgcgc
     1921 ctcgagctcc tgcaggcgcg cctccgcgcc ggccggctcg gcctctgcca tccgtgtcgc
     1981 catcgcctcg cccctccccg ccgggcgctc cccggcattg tcccgcgggt cggccgctcc
     2041 gtcgaccttt gcccggctag tccaggtggt acggcacgct gaccaggacc gtgcgcggct
     2101 cgaacagcag caggcgtttg atgaacaggg cggtgttgtt gtgcagcagt cgctgccaga
     2161 gcttcgggac cacgaactcc ggcagcacga ccacggccag cgagtggggg gcggcggtgg
     2221 ctcgccggat ctcctcgagc accggcaccg agaggtcgcg gaacggcgcg ccgacgatct
     2281 cgagcgggat gtcggcgccg gcgcggaccc agtcctcctc gatccgctcg atctcctcgg
     2341 ggtcgagcga gatgaagacc gccttcgtct cggtggcctg cagcgagcgc gcgtagttga
     2401 cggcgcgaag ggtcgcgtcg tgcacggagg cgaccagcac gatggtggtc accgacgtcg
     2461 ggatcagcgg ccgggcgtcg acgccgaccg gctgcccctc caccacctgg accggcgcgt
     2521 tcatcacggc cacgccgcgc tccggcagca gtcggagctt gatccgggtc gccggggaca
     2581 gcgccaggag gaacaggatc gaccggcgcg ggaccatctc cgggacgacc acggtgacga
     2641 agtcctctgc cccccgctcg atcgtccgga cgaagtcgcg gatcgcggcc ccggggtggt
     2701 cgcgggaggg gaccacctcg agcggcgcgc caccgcccga gaaacggcgc cagcgttcct
     2761 cgagatcagg cggagtgtct ccggtctcca ggtacacgta ccggacgggt agcccgcgga
     2821 gcgaccgcac gtaccccagg gcctcggcgg cggcagcatc caggtccgtc atcgccagga
     2881 cgatcgtgtt cgcgggaatc tccccgacgt ggacccgacc ccgtcggagc tgtgcggacc
     2941 aggacgcata gtgccggttc acgccgagca tcaacaggat catgaccggc atggccgaga
     3001 tcacgatcca cgcgccgtgc gtgaacttcg tcagggtgac gatgaccagc acgatgccgg
     3061 tggcgaacgc gccgatcgcg ttgatcacgg cgctccgctt ccaaccgggc tcgcgggtgc
     3121 gcagccatcg ccggaccatc ccggcctgcg acagtgtgaa cgccgtgaac acccccacca
     3181 cgtacagctg gatcagccgg gtcagctcgg cgtcgaacgc ccacaccagc gccgcggcga
     3241 ccagggccag cacgacgacg ccgttcgaga acaccaggcg gtcgcccctg ttgcggaacg
     3301 gcgtcggcat gaagcggtcg cgggcgagga tcgaggacag gcgcgggaag tcctggtagg
     3361 cggtgttcgc cgcgaggatc aggatcaggg ccgtgaacgc ctgcaggacg tagaacatca
     3421 acccggagcc gaagagggtc gcgccgatct gcgcgagcac ggacttctcg ccgacgagcc
     3481 cctcgctgac gtggacgtcg aacgccctcg ccaggaaggt gatgccaagg aacatcgaga
     3541 tcgagatggc gcccatcacg gcgagcgtct tggcggcgtt gcgggactgc gggcggcgga
     3601 atgccggcac gccgttggag atggcctcca cgccggtcag cgccgtcgat cccgacgaga
     3661 acgctcgcag gatgaggtag agcgacacgc cctggaccga ctccaccggc aggctcgccg
     3721 aggccgccgt cgggcacccg ttcaggcagt tgacgaaccc gacgccgagc gtgacgtaca
     3781 cgcaggcgac gaacccgtag gtcgggacgg cgaagagggt gccggcctcc ttcaccccgc
     3841 gcaggttcgc cagcgtgatc agggcgatga acaggatcga catctccacc cgcaggtgcg
     3901 tcagcccggg ggccgccgag gtgatggcgg ccaccccggc caccacggag accgagacgg
     3961 tcaggacgta gtcgatcagc agcgccgagg ccgagatcag gccggggagg gtccccaggt
     4021 tctcgtgcgc cacgatgtag gagccgccgc cgcccggata ggcgcggacg gtctgccggt
     4081 acgacgtgat cacgatcgcc agcaccgcgg cgatcaccag cgcgatcggc agcatgaacg
     4141 cgaacgcggc ggtgccggcg agtgccagca ccaacatcat ctcctcggtg gcgtaggcga
     4201 ccgaggacag cgggtcggag gagaacaccg ggagcgccag gaacttcggc aacagggtgt
     4261 gctcgaggtg gtgggatggc ttggcccggc cgaccaggat ccgcttgatc accccgccga
     4321 cgccgacgcg agtgcgctcc tcgagcctgg ttgccacggc ggggattcta ggtccgccga
     4381 gccacccgcc tcagccgccc ggctccgccc agcggtaccc gacgcccggc tcggtgagca
     4441 gccagcgcgg ccggcccggc tccgcctcga gcttcttgcg cagcgcggcg acgaacaccc
     4501 gcaggtagtg gctctcctcg ccgtagcccg ccccccacac ccggccgagg agccagcggt
     4561 gggtgaggag cttgccgggg tgcgtgacga aggcctccag cagctgccac tcggtcgggg
     4621 tcaggtggat cggctcttcg ccgcgagtca cccggtgctt cggtaggtcg accaccaggg
     4681 ggccgaagcg cagcaccggc tcggtcgcct ccgcggtgcc cgcccggcgg ccgacggcgc
     4741 gcatccgagc cagcagctcc tccatggcga agggtttcgt cacgtagtcg tccgcaccgg
     4801 catcgagcgc ggccaccttg tccgcctgcc cctcgcgcac cgacagcacg atgatcggga
     4861 ccgtcgagaa cgatcggatc cgccgcgtcg cctcgatccc gtcgatcccg ggcagcccga
     4921 gatcgaggac gacgaggtcg gggtcggcgg aggcgaccag gtccagcgcc gtctcgccgt
     4981 tggcggcgac gagcacctcg tggccgcgcg ccgagaggct ggtccgcagc gcgcgcagga
     5041 tctgcggctc gtcgtcgacc accagcaccc gggtcatcga ggttcctccc cgtcccgggt
     5101 tgccggcagc tccagcacga tcgtcgtccc ccccgccggg gtcccctccg cccagatccg
     5161 gccctcgtgc accgagacca cggcgcgggc gatcgccagg ccgagcccgc tgccgggcgc
     5221 ggcgcggtcg gcgtcccgcc ggtagaaggg ctcgaagacc tgctcccgct ccggctccgg
     5281 gatcccctcg ccgtggtcga ccacgcggac ctgcacgccg tcgcggaacc gggaggcggt
     5341 gacctcgatc gccgtccccg agggcgagaa cttcacggcg ttctccagaa ggttcgtcag
     5401 cacctggtcg aactggacga cgtcgagcgg caggtcgggc aggtcctccg ggacgcggac
     5461 ctcgaccggc ctgccctcca ggaccggccg cagccgggac aggaccccgc cgatcacgtc
     5521 ggccaccgcg acgggctcgc gcgcgggcac cagcgcaccg gcgcgcatcc gcgagaggtc
     5581 gaggaggttc ccgaccatcc gattcaggcg gtccgtctcc tcgaggatcg tctgcagcag
     5641 ctcgccgcgc tcgccgcggt cgaaatcgac cgcctcgtcc tgcaggctcg tcaccgaggc
     5701 cttgatcgac g
//
LOCUS       DASPWO010000461         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_997229, whole genome shotgun sequence.
ACCESSION   DASPWO010000461 DASPWO010000000
VERSION     DASPWO010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997229"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2824
                     /locus_tag="VEO00_10470"
     CDS             <1..2824
                     /locus_tag="VEO00_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYU58458.1"
                     /translation="HVATFLEWAPIRLKGKARPIVAYQVQGVAALPGGRRPSPVATPF
                     VGRAAELAWLHDLLASAIGERTSRIALVAGVAGVGKTRLAEAFVAGAPDAVHLAGACV
                     PFGERTPWHALASALRDHARIAPSDGAEQTRRKLGSLVGAAVPASGTAERAALVRDVA
                     TVLGLEPDPAPPSATELNLDLAQAVRRTISGLAAERPVVVTLEDVHWADPQLLAFLRW
                     VAEEPWPAPVLMLCLGWSESLGQVPVDPRDARSLSSLGADDARRLLGSLLPGDIPPTL
                     AAELLDRSGGNPLFLEELVRLLSENRRLKRDGDSWHVEGGLSEAVPETVQLVVAARLD
                     GLSGEERRLCRDAAIAGDRFWDALLRALGWGDGLDALLDRLAVRDLIREQEPSGVPGT
                     REFAFKHFVIRDVAYASVPRADRAGRHLAIADWLRSSARPGDEPVDLLAFHYGQAVLL
                     GRESVRAHLPVALDYLRRAASRAERRSAHREAVAVCEQALDAVSWVHLDPASPEAALL
                     AEILLVHAQALVFLSRHQEARAAAAQVEEVARASGRRDLEAHALVARGRVEADLPNVE
                     AALPLFESAAALYRTLGDRHGQAIVLSETSYAHRLDDLVRQRDLLERATLAFAEIGDF
                     WWELRCCQSMAWLQSPRGGEDFLRWYRTLESLTARAGDRRSVAGLRRAWGYFRLYAGD
                     LEDARTAMADALAIGREIGDLDHELESRFPLGLIALALGDVEEAARHAAPLSRVGRRR
                     GWARTEAEGMVLEARVASRRGRRDEADAGLARAEATLASIRQERELSEVTVARTEVAL
                     DGGAWTEAAGLAERFVEENRRFDDRLVLPHALGLVALARLGAGDLEGAEESARSAAGE
                     AQRAGNVLVPARAACVSAVARALRGEPPGDLPAELPTPVERALAAEARALSGEPGGWE
                     DAAAAWSALGETAWRDRSRELAGRSE"
     gene            2976..>3384
                     /locus_tag="VEO00_10475"
     CDS             2976..>3384
                     /locus_tag="VEO00_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU58459.1"
                     /translation="MASARAANELRGSIGTTPSDGSAILTADAVRKTYRTGTVEVEAL
                     AGVDLTVNRGELVSVMGPSGSGKTTLLNCLSGLDDIDGGRVVIDGEDLFAMSDTARTM
                     HRARSMGFIFQAFNLIPVFTVAENVELPLLLVGT"
BASE COUNT          371 a         1237 c         1321 g          455 t
ORIGIN      
        1 gcacgtcgcc acgttcctcg agtgggctcc gatccggctg aaggggaagg cccgcccgat
       61 cgtcgcctac caggtgcagg gcgtcgccgc gctccccggc gggcgccggc cctcgccggt
      121 cgccacgccg ttcgtcgggc gggcggccga gctcgcctgg ttgcacgacc tgctcgccag
      181 cgcgatcggc gagcgcacca gtcggatcgc cctggtcgcc ggcgtggccg gcgtggggaa
      241 gacgcgcctg gcggaggcct tcgtcgcggg ggcgccggac gccgtccacc tggccggcgc
      301 gtgcgtcccg ttcggcgaac gcacgccgtg gcacgcgctg gcttccgccc tgcgcgacca
      361 cgcccggatc gctccgtcgg acggggcgga gcagacgcgg cggaagctcg gctcgctggt
      421 cggggccgcg gtccccgcgt cggggacggc cgaacgcgcc gcgctggttc gcgacgtggc
      481 gacggtcctc ggcctcgagc ccgacccggc gcctcccagc gcgaccgagc tgaacctgga
      541 cctggcccag gcggtccggc ggacgatctc tggcctcgcg gccgagcgcc cggtggtcgt
      601 gacgctggag gacgtccact gggcggaccc gcagctgctg gcgttcctgc gttgggtggc
      661 cgaggagccc tggccggccc cggtgctgat gctgtgcctg ggatggtcgg agtcgctcgg
      721 ccaggtgccg gtcgatcccc gggacgcgcg atcgctctcc tcgctcggag ccgacgacgc
      781 gcgccggctg ctcggctcgc tgcttccggg ggacatcccc ccgaccctcg ccgccgagct
      841 gctcgaccgc tcgggtggca acccgctgtt cctggaggag ctcgtgcggc tgctctcgga
      901 gaaccgccgc ctgaagcgcg acggcgactc ctggcacgtc gagggaggcc tgtccgaggc
      961 ggtacccgag accgtccagc tcgtggtggc ggcccggctg gacggattgt ccggtgagga
     1021 gcgccggctc tgccgcgacg cggccatcgc cggcgaccgc ttctgggacg cgctccttcg
     1081 ggcgctcggg tggggcgacg ggctggacgc gctgctcgac cgcctggcgg tgcgggacct
     1141 gatccgggag caggagccga gcggggtccc cgggacgcgc gagttcgcct tcaagcactt
     1201 cgtgatccgc gatgtggcgt acgcgtcggt gccgcgggcc gaccgcgccg ggcgccacct
     1261 ggccatcgcc gactggctgc ggtcctcggc ccgtcccggc gacgagccgg tcgacctgct
     1321 ggccttccac tacggccagg cggtgctgct cggacgcgag agcgtccggg cgcacctgcc
     1381 cgtggcgctc gactacctgc ggagggcggc gtcccgcgcc gaaaggcgct cggctcaccg
     1441 cgaggcggtc gccgtctgcg agcaggccct ggacgcggtg tcgtgggtgc acctcgatcc
     1501 ggcgtcgccg gaggcggcgc tccttgccga gatcctcctg gtgcacgccc aggcgctcgt
     1561 gttcctgtcc cgccaccagg aggcccgggc ggcggcggcc caggtggagg aggtcgcccg
     1621 ggcgtccggc cgcagggacc tggaggcgca cgccctggtg gcccgcggcc gggtcgaggc
     1681 ggacctgccc aacgtcgagg ccgccctgcc cctgttcgag tccgcggcgg ccctctaccg
     1741 tacccttggc gaccggcacg gccaggcgat cgtgctctcc gagacctcgt acgctcatcg
     1801 cctcgacgac ctggtccggc agcgggacct gctggagcgc gccaccctcg ccttcgcgga
     1861 gatcggcgac ttctggtggg agctgcgctg ctgccagtcg atggcctggc tccagtcccc
     1921 gcgcggcggc gaggacttcc tgcgctggta tcgaacgctc gagtcgctga cggcacgggc
     1981 gggcgaccgt cgctcggtgg ccgggctccg gcgcgcctgg ggctacttcc ggctgtatgc
     2041 cggcgacctg gaggacgccc ggacggcgat ggcggacgcc ctcgcgatcg ggcgcgagat
     2101 cggcgacctc gatcacgagc tggagtcacg gttccccctc gggctgatcg cgctggcgct
     2161 cggcgacgtc gaggaggcgg cgcgccacgc ggcgccgctc tcccgggtgg gccggcgccg
     2221 cggctgggcc cggacggagg cggagggcat ggtgctcgag gctcgagtgg cgtcgcggcg
     2281 cggccgccgc gacgaggcgg acgcgggcct cgctcgcgcc gaggccaccc tcgcctcgat
     2341 ccgccaggaa cgcgagctgt cggaggtcac ggtcgcccgg accgaggtgg cgctcgacgg
     2401 tggggcctgg acggaggccg ccgggctcgc ggagcgcttc gtcgaggaga accgccgctt
     2461 cgacgaccgg ctggtgctgc cccacgcgct cgggctggtc gccctggcac ggctcggcgc
     2521 gggcgacctc gagggcgccg aggagtcggc gaggtcggcg gccggggagg cccagcgcgc
     2581 ggggaacgtg ctggttccgg cgcgggcggc ctgcgtgtcg gccgtggcgc gcgcgctgcg
     2641 gggcgagccg cccggagacc tcccggccga gctcccgacg ccggtcgagc gcgcgctggc
     2701 cgccgaggct cgcgcgctgt ccggcgagcc cggcggctgg gaggacgccg cggcggcctg
     2761 gtcggcgctc ggcgagacgg cctggcgcga ccgcagccgc gagctggccg ggcgctccga
     2821 gtagcgacgg ttccccccgg ccctcccccc gttccccacc ggtcccgaac ggggcgggac
     2881 catttgcatc atggttcccg cggccgggtg agcgacgatc cggcgcaagg tgcgcgcatg
     2941 gtcgcgcggg ccacaggacg agaaaggggc gcaccatggc gagcgcgcga gctgcgaacg
     3001 aactgcgggg atcgatcggc acgactccat cggacgggtc ggccatcctg accgccgacg
     3061 ccgtccgcaa gacctaccgg accggcaccg tcgaggtgga ggccctcgcc ggggtcgacc
     3121 tgacggtgaa ccgaggggag ctggtgtcgg tgatggggcc ctcgggctcc ggcaagacca
     3181 cgctcctgaa ctgcctgtcg gggctcgacg acatcgacgg cggacgggtc gtgatcgacg
     3241 gcgaggacct gttcgcgatg tcggacacgg cgcggaccat gcaccgcgcc cgctcgatgg
     3301 ggttcatctt ccaggcgttc aacctgatcc cggtcttcac tgtggccgag aacgtcgagc
     3361 ttcccctgct gctggtcggc acct
//
LOCUS       DASPWO010000462         9624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_659524, whole genome shotgun sequence.
ACCESSION   DASPWO010000462 DASPWO010000000
VERSION     DASPWO010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659524"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VEO00_10480"
     CDS             complement(<1..290)
                     /locus_tag="VEO00_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58460.1"
                     /translation="MIQDARSCNGDHTFRFIDTVSQGNHEFLSSQYNNGWFHYAIDGN
                     YYDQPYVRTPNWVAGHPNAGGEIRYSCDQDTTRWWGLKMREPIAAGGDWVTW"
     gene            complement(444..1541)
                     /gene="ispG"
                     /locus_tag="VEO00_10485"
                     /gene_synonym="gcpE"
     CDS             complement(444..1541)
                     /gene="ispG"
                     /locus_tag="VEO00_10485"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HYU58461.1"
                     /translation="MTERRRSRQVRVGWVEVGGDAPVRVQSMTTTKTADVDATLQQMA
                     ALVAAGCEIVRVAVPHREDAEALKTIAEHATIPVIADIHFQWKYAMAALEGGIHGLRI
                     NPGNIKYQDKVRLIAREAKARGVPIRIGVNAGSLEKALLAKHGKPTAEALVESALDEA
                     RILEDEDFTDIKISVKHSNPRVMIDAYRLLAAACDYPLHLGVTEAGPMPIGGVKSAVG
                     IGTLLAEGIGDTIRVSLTDDPVEEVKVGNAILQSLGLRRRGLDLVACPSCGRAEVDVL
                     GLTKQVNEALEREGLKAPLRVAVMGCVVNGPGEAREADLGIAAGKGYGQLIVRGEVQE
                     HRIPEDRIVEVVVAKARELAEDMVAEGASRD"
     gene            complement(1604..2791)
                     /locus_tag="VEO00_10490"
     CDS             complement(1604..2791)
                     /locus_tag="VEO00_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HYU58462.1"
                     /translation="MPALIGVLIYIGLTVLFSLTGQAVIAGTLIFVGAVILVILFHEA
                     GHFASAKAFGIKVDEFFIGFGPRLWSVRRGETEYGVKAIPAGGYVRIAGMNPYEETPV
                     EDLPRTFGAKPRWQRAIVLVAGALTHFVLAFLLLVVFFSAIGQEVALPRIDEVVQELD
                     DGSPTPASTAGFRAGDVLVSVDGVAIESWDQARDYISAHPGQRITFVVERDGRRLTIA
                     VTPASIESGGETLGFIGLAPVVETRKDNVIVAFGHAGTTLVDLVGQSFKAIARVFGPE
                     GLGRIGRLLIGRAERDVTDPVGLVGAARLSGQAVAGGAVGALIGLLAGFNVFVGIVNL
                     LPLPPLDGGHLAVLAIEGVTGKRIDPRRLVPITALVAGFLILFTISVLYLDIASPIPN
                     PFR"
     gene            complement(2795..3958)
                     /locus_tag="VEO00_10495"
     CDS             complement(2795..3958)
                     /locus_tag="VEO00_10495"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HYU58463.1"
                     /translation="MRSLTILGSTGSIGTQALDVVRRFPERFRVAGLGAGSNHDLLVG
                     QIREFLPPVVAIADDGGVDELRARLRGIREPQILAGADAAERLARDTDADMIVNAMVG
                     AVGLEPTLAALSSGKTVALANKESLIVGGELVMDLVKAEPERLIPVDSEHSALAQCLR
                     GERRDDLRRVIITASGGPFRGWTQDELRRASVKEALAHPTWNMGEKITIDSATLMNKG
                     LEVIEAHYLFGLDYAEIEVVVHPESIVHGLAEFRDGSVLAQLATPDMRLPIQLALASP
                     DRLGSGVRPLDLVSIGRLTFEAVDREAFPALDLAVQAGRSGGTFPAVLNAANEIAVMA
                     FLESKIALTGIADVVASVLEEHDGARVVSAVALERADRWARRRAAEVIDQRPR"
     gene            complement(4050..5900)
                     /locus_tag="VEO00_10500"
     CDS             complement(4050..5900)
                     /locus_tag="VEO00_10500"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HYU58464.1"
                     /translation="MPSPEDERGRSGSGDLFEDLDDFFAPIEETDWPEPDPGGPPVAP
                     RAAPRPPAPPEGGDEEDLLPEGWGEAIDLGDIPAPAGSATSFSLDEEEEGPALGDLLA
                     ASREEPAEAAPAERAEEAAEEAAFPERPAWERGTEGEEGAGEEAPVFIDDQAQPTAAG
                     EPSVWEALEPSTGVGAEEDVYLIEAEEEGVAAAAGEPGGGTRELSVEDLRATPPQYAE
                     LPGPPEEDAGAEAEEGPPARTPAEPTFAGPAFEEPGFTYEEEQEPLMPAAAARPAAPG
                     EESGDVEAAAEHFAEGMRRSPDEVERELLADMEPGAAGPPRTVRVGEPPAEAGPSWEE
                     PASQPVAAERPRAPVVGRNLPAALITGVGLAALALVLLAIGPAPFAILAGGVVLLAQG
                     ELYAVVRRRGYQPATALALVCGGLMVFAGYNKGESAMAFILFLSLALTVAWYMAAPVK
                     ARRGTVPNIAMTMLGLIYVPFAAAFIFRILPLPTGRAIALSILGLTVLYDVAAFGVGS
                     LWGRRPLAPTISPNKSWEGLIGSSLVTIVVAVAIVASIDPFEIGSALGLAAVVLVAAP
                     LGDLAESVLKRDLGVKDMGTIMPGHGGLLDRIDSLLFVIPAAYYFLRIVL"
     gene            complement(5971..6528)
                     /gene="frr"
                     /locus_tag="VEO00_10505"
     CDS             complement(5971..6528)
                     /gene="frr"
                     /locus_tag="VEO00_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HYU58465.1"
                     /translation="MIDDTLRDAEQKMRRAVEVTQDEFAGVRTGRATPKILDRVMVEY
                     YGSPTPLNQLASFSVPEPRLLVIQPYDRGAMTAMEKAIMSSDLGLTPSNDGNVIRLAF
                     PQLTEERRKELIKVVHHRAEEGRVAVRNVRRHHKEELERLERDREISEDDLRRAEKEL
                     QKLTDRFVAEIDELLEHKEQELLEV"
     gene            complement(6542..7285)
                     /gene="pyrH"
                     /locus_tag="VEO00_10510"
     CDS             complement(6542..7285)
                     /gene="pyrH"
                     /locus_tag="VEO00_10510"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HYU58466.1"
                     /translation="MADADPVAGLRPAYRRVLLKLSGEAFSPPGREFGISSEALGNVA
                     RQLAEVVADGVETAVVVGGGNIWRGRDHQSMDRARADYIGMLATVMNALALQDALENI
                     GVITRVQTAIAMAQIAEPYIPRRAMRHLEKGRVVIFAAGMGAPFFSTDTTAAQRALEI
                     KAEAILKGTSVDGVYDADPRLQPDAVKFETLEYIEVLKRGLQVMDATAISLAMENRLP
                     IIVFNLWEDGNIRRAVAGEPIGTLVRAGA"
     gene            complement(7297..7905)
                     /gene="tsf"
                     /locus_tag="VEO00_10515"
     CDS             complement(7297..7905)
                     /gene="tsf"
                     /locus_tag="VEO00_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HYU58467.1"
                     /translation="MSDPKITAEEVRVLRELTGAGMMDCKRALQDAGGDLARAQEILR
                     ERGLAAAGKRAGRSANEGVIEAYIHPPGKLGVLVEVNSETDFVANTEEFGTLARNVAM
                     HIAALAPRWISRDDVPEDVVLAERKIYAEQARTTGKPEHVLEKIVEGKLRAFFKQTVL
                     LDQPFIRDDSVSVGDLVARVSAKVGEKIAVRRFARFKLGEEA"
     gene            complement(7902..8912)
                     /gene="rpsB"
                     /locus_tag="VEO00_10520"
     CDS             complement(7902..8912)
                     /gene="rpsB"
                     /locus_tag="VEO00_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HYU58468.1"
                     /translation="MSVVTRRELLEAGVHFGHQTRRWNPKMRRFIFAERSGIYIIDLE
                     QSLTGLQRAHAFVRELARKGGSILFVGTKKQAQEVVADQASRIGMPYVNTRWLGGMLT
                     NFQTISQRLARLRELQDMERSGAFEYLPKKEVLKLRHEKEKLERNMGGIQTMTRRPDA
                     ILVIDTKKEHIAVTEARKLGIPVVAIVDTNCDPDEVDYVIPGNDDAIRSVALVTKIIA
                     DAFEEGAFLGGEEIARAARGEPEFQPEPMELEPATTLPESQVFEPGPPDAVGVDRSAG
                     AGPESAGEAGAEVPPEAQPDVVPESSGEVAAGAPGETSDRTTSGERPPLDRKTGVGES
                     TE"
     gene            complement(9102..>9624)
                     /locus_tag="VEO00_10525"
     CDS             complement(9102..>9624)
                     /locus_tag="VEO00_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58469.1"
                     /translation="LFGLVIALMAPDLGWSEERPLHAWRRAIPHLVLVALALFGVAGA
                     IPLLLQGFAPQFTGFVQLGAPLIGLYLLGFAPIAAVVDGDPYREAVRRSIRAARLPGG
                     GHLSFILLYFAFVFFLFAGVPGGPATPLTPSVATWGFTLIGSLFHVGVLGALTFRWLA
                     VRDRLAEAPPPRR"
BASE COUNT         1378 a         3553 c         3292 g         1401 t
ORIGIN      
        1 taccacgtca cccaatcgcc gcccgcggcg atcggctcgc gcatcttgag accccaccaa
       61 cgcgtcgtgt cctgatcgca cgagtacctg atctcgcccc cagcattcgg atggccggcg
      121 acccagttgg gcgtccgaac gtacggctgg tcgtagtagt taccgtcgat ggcgtagtgg
      181 aaccaaccgt tgttgtactg ggagctcaga aactcatggt tgccctgcga aaccgtgtcg
      241 atgaaccgga acgtatgatc tccgttgcac gagcgcgcat cctggatcac gactgctcga
      301 ccagcagcgc cgccacccca ccatgggcct tcctcacagg gacaatcggg acgatatgtc
      361 gcggttcatc gccggtcgag ggtccgactt gaccaacccc gtctccacgc cgcaggcccc
      421 tccacgttcg gcaggccggc aggtcagtcc cgagaagcgc cctccgccac catgtcctcg
      481 gcgagctcgc gggccttggc caccaccacc tcgacgatgc ggtcctcggg gatgcggtgc
      541 tcctggacct ctccgcggac gatcaattgg ccgtagccct tgccggcagc gatgcccaga
      601 tcggcctcgc gcgcctcgcc cgggccgttc accacgcagc ccatcaccgc gacgcgcagg
      661 ggcgccttca gcccctcgcg ctcgagggcc tcgttcacct gcttcgtcag tccgaggacg
      721 tcgacctcgg cacggccgca cgacgggcag gccaccaggt ccaggccgcg ccggcgcagg
      781 ccgagcgatt gcaggatcgc gttcccgacc ttgacctcct cgacgggatc gtcggtcagc
      841 gagacccgga tcgtgtcgcc gatcccctcg gccagcagcg tcccgatccc gacggccgac
      901 ttcacgccgc cgatcggcat cggcccggcc tcggtgacgc cgagatgcag cgggtagtcg
      961 caggcggcgg cgagcagccg gtacgcgtcg atcatcaccc gcgggttgga gtgcttcacc
     1021 gagatcttga tgtcggtgaa gtcctcgtcc tccaggatcc gcgcctcgtc gagggccgac
     1081 tccaccaggg cctcggccgt cggcttgcca tgcttcgcca gcagcgcctt ctcgagcgag
     1141 ccggcgttca ccccgatccg gatcggcacg ccacgggcct tcgcctcgcg agcgatcagg
     1201 cgcaccttgt cctggtactt gatgttgccg gggttgatgc gcaggccgtg gatgccgccc
     1261 tcgagcgcgg ccatcgcgta cttccactgg aagtggatgt cggcgataac cgggatcgtg
     1321 gcgtgctcgg cgatcgtctt cagagcctcg gcgtcttccc tgtgcggcac cgccactcgc
     1381 acgatctcgc agccggccgc gacgagcgcc gccatctgct gcagcgtcgc gtcgacgtcg
     1441 gccgtcttcg tggtggtcat cgactggacg cgcaccggcg cgtcgccgcc gacctccacc
     1501 cagcccacgc gcacctgtcg tgaacggcgc cgttcggtca tcgccttcag tctacgcggg
     1561 ggccgtcgag cgaccgtggg gacgtgtgca ggaaccgtgc aggtcagcgg aacgggttcg
     1621 ggatcgggct ggcgatgtcc aggtacagga ccgagatcgt gaacaggatc aggaaccccg
     1681 ccaccagcgc ggtgatcggg accaggcggc gcgggtcgat ccgcttgccg gtgacgccct
     1741 cgatcgccag gacggccaga tggccgccgt cgagcggcgg cagcggcagc aggttgacga
     1801 tgccgacgaa cacgttgaag ccggccagca gaccgatcaa cgcgccgacc gccccaccgg
     1861 ccacggcctg tccggagagg cgcgccgcgc ccaccaggcc gacggggtcg gtcacgtcgc
     1921 gctccgcccg cccgatcagg agccttccga tccggcccag gccctcgggc ccgaacacgc
     1981 gggcgatggc cttgaacgac tgcccgacga ggtccacgag cgtggtgccc gcgtgcccga
     2041 acgcgacgat gacgttgtcc ttccgggtct cgacgaccgg cgccaggccg atgaacccga
     2101 gtgtctcgcc gcccgactcg atcgacgccg gggtgaccgc gatcgtcagg cgccgtccgt
     2161 cgcgctcgac gacgaaggtg atgcgctgcc cggggtgcgc ggagatgtag tcgcgcgcct
     2221 ggtcccagct ctcgatcgcc acgccgtcga ccgacaccag gacgtccccg gcgcggaatc
     2281 cggccgtgga cgccggcgtc ggcgacccat cgtcaagttc ctgtaccacc tcgtcgatgc
     2341 gcgggagggc aacctcctgc ccgatcgcgg agaagaacac cacgaggagc aggaacgcca
     2401 gcacgaagtg cgtcagggcg ccggccacca gcacgatcgc ccgctgccag cgtggcttgg
     2461 ccccgaacgt gcgcgggaga tcctcgacag gggtctcctc gtacgggttc atcccggcga
     2521 tgcgcacgta gccgcccgcg gggatggcct tcaccccgta ctcggtctcg ccgcggcgca
     2581 ccgaccacag gcgcgggccg aacccgatga agaactcgtc gaccttgatc ccgaacgcct
     2641 tggcggaggc gaagtggccg gcctcgtgga acaggatcac caggatcacc gcgcccacga
     2701 agatcagcgt cccggcgatg accgcctggc cggtcagcga gaacaggacg gtcaggccga
     2761 tgtagatgag gacgccgatg agcgccggca cggtctagcg gggccgctgg tcgatcacct
     2821 cggcggcgcg ccggcgggcc cagcgatccg cccgctccag cgcgaccgcc gacacgaccc
     2881 gggccccgtc gtgctcttcc aggacggagg ccaccacgtc ggcgatcccg gtcagcgcga
     2941 tcttgctctc gaggaacgcc atcacggcga tctcgttcgc ggcgttcagc acggcgggga
     3001 acgttccccc ggagcgcccg gcctgcaccg ccaggtcgag agccgggaac gcctcgcgat
     3061 cgacggcctc gaacgtcagg cgcccgatcg agaccaggtc gagcggccgc acgccggagc
     3121 ccagccgatc cggggaggcc agcgcgagct ggatcgggag gcgcatgtcc ggcgtcgcca
     3181 gctgcgccag cacgctcccg tcgcggaact cggccagccc gtggacgatc gactcggggt
     3241 gaacgacgac ctcgatctcg gcgtagtcga gcccgaagag gtagtgggcc tcgatcacct
     3301 cgaggccctt gttcatcagc gtcgcggagt cgatcgtgat cttctccccc atgttccagg
     3361 tcgggtgcgc cagcgcctcc ttgaccgatg cccgccgcag ctcgtcctgg gtccagccgc
     3421 ggaacggccc gccggaggcg gtgatgatca cgcgccgcag gtcgtcgcgg cgctccccgc
     3481 gcaggcactg ggccagggcc gagtgctcgg agtccaccgg gatcaaccgc tcgggctccg
     3541 ctttgaccag gtccatcacc agctcgccgc cgacgatcag ggactccttg ttggcgaggg
     3601 ccacggtctt gccgctcgag agcgcggcga gcgtcggctc gagcccgacc gcgccgacca
     3661 tggcgttgac gatcatgtcg gcgtcggtgt cgcgggccag ccgctcggcg gcgtcggcac
     3721 cggccaggat ctgcggctcg cggatgccgc gcaggcgggc gcggagctcg tcgacgcccc
     3781 cgtcgtcggc gatcgccacg accggcggga ggaactcgcg gatctgcccg accagcaggt
     3841 cgtggttcga gccggcgccc aggcccgcca cccggaagcg ctccgggaag cggcggacca
     3901 cgtccagcgc ctgggtgccg atggagccgg tcgagccgag gatcgtcagc gagcgcatca
     3961 cggatgaggg tacccgcttg gcgacggaat gttcggcagg cgggccacgg gcggccgccg
     4021 gcgccatccc gcccggccgg ccgacgcgcc tacaggacga tgcgcaggaa gtagtaggcg
     4081 gcggggatga cgaacagcag tgagtcgatc cggtcgagca ggccgccgtg gccgggcatg
     4141 atcgtcccca tgtccttcac gccgaggtca cgcttcagga cggactcggc caggtcaccg
     4201 aggggggcgg cgaccagcac caccgccgcc agcccgagcg cggagccgat ctcgaacggg
     4261 tcgatcgagg cgacgatggc cacggcgacg acgatcgtca ccagcgagct gccgatcagc
     4321 ccctcccacg acttgttcgg gctgatcgtc ggcgccagag gacggcggcc ccacagcgag
     4381 ccgaccccga acgccgcgac gtcgtagagc accgtcagcc cgaggatcga tagggcgatc
     4441 gccctcccgg tgggcagggg caggatccgg aagatgaagg ccgccgcgaa cgggacgtag
     4501 atcaggccga gcatcgtcat cgcgatgttc gggaccgttc cccggcgggc cttcacgggc
     4561 gccgccatgt accaggcgac ggtgagggcc agggagagga acaggatgaa cgccatcgcc
     4621 gactcgccct tgttgtagcc ggcgaacacc atcaggccgc cgcagaccag ggcgagcgcc
     4681 gtggccggct ggtagccgcg gcggcgaacc accgcgtaca gctccccctg cgccagcagc
     4741 acgacgccgc cggcgaggat cgcgaagggc gccgggccga tcgccagcag cacgagcgcc
     4801 agcgcggcga gccccacgcc ggtgatcagg gcggccggca ggttgcggcc caccaccggc
     4861 gcccggggcc gctcggcggc gacgggttgg gaggccggtt cctcccagga cggaccggcc
     4921 tcggcgggcg gttcgccgac gcgtacggtg cggggcgggc ccgcggcgcc cggctccatg
     4981 tcggcgagca gctcgcgctc gacctcgtcc gggctccggc gcatgccctc ggcgaagtgc
     5041 tcggccgcgg cctcgacgtc accgctctcc tcgcctgggg cggccggccg ggcggcggcc
     5101 gcaggcatca gcggctcctg ctcctcctcg tacgtgaagc ccggctcctc gaacgcgggg
     5161 cccgcgaagg tcggctccgc cggggtccgc gccggcgggc cttcctcggc ctcggctccg
     5221 gcatcctcct ccggcggccc tgggagctcg gcgtactgcg gcggcgtggc gcgcaggtcc
     5281 tcgaccgaca gctcgcgggt gcctccgccc ggctcgccgg ccgcggcggc aacgccctcc
     5341 tcctcggcct cgatcaggta gacgtcctcc tcggcgccga ccccagtgga cggctccagg
     5401 gcctcccaga ccgagggctc gccggccgcg gtcggctgcg cctggtcgtc gatgaagacg
     5461 ggggcctctt ccccggctcc ctcttcgccc tcggtccccc gctcccaggc cgggcgctcc
     5521 ggaaacgctg cctcctcggc cgcctcctcg gcccgctcgg ccggggcggc ctccgccggc
     5581 tcctcgcggg aggccgccag caggtcgccg agcgccgggc cctcctcctc ctcgtcgagg
     5641 gagaaggacg tcgccgagcc cgccggggcc gggatgtcgc cgaggtcgat cgcctcgccc
     5701 cacccctcgg gaagtaggtc ctcctcgtcg cccccctccg gcggggcggg cgggcgcggc
     5761 gcagcgcgcg gcgcgaccgg cggcccgccg gggtcgggct cgggccagtc ggtttcctcg
     5821 atcggggcga agaagtcgtc caggtcctcg aacagatccc cggacccgga acgcccgcgc
     5881 tcgtcttcgg ggctcggcat gcgatcgcct ctccctctcc gagagccctc acgcttcccc
     5941 agtggcccgg gtcaccgggc ccgagagggt tcagacctcc aagagctcct gttccttgtg
     6001 ttcgagcagc tcgtcgatct cggcgacgaa ccgatcggtc agcttctgga gctccttctc
     6061 cgctcgccgg agatcatctt cagagatttc gcggtccctc tcaagccttt ccagctcttc
     6121 tttgtggtgg cgccgcacgt tgcgtacggc gacgcgtccc tcttccgcac gatggtggac
     6181 gaccttgatc agctccttgc ggcgctcctc ggtgagctgc gggaacgcca ggcggatgac
     6241 gttgccgtcg ttcgacggcg tcaggccgag gtccgagctc atgatcgcct tctccatcgc
     6301 ggtcatggcg ccccgatcgt agggctggat gaccaggaga cgcggctcgg gaacggagaa
     6361 cgacgccagc tggttgagcg gcgtcggcga cccgtagtac tcgaccatca cccgatcgag
     6421 gatcttcggc gtcgcccgcc cggtgcgcac gccggcgaac tcgtcctgcg tcacctccac
     6481 cgcgcggcgc atcttctgtt cggcgtcccg cagcgtgtcg tcgatcatac cggccttccg
     6541 ctcaggcccc cgcacggacg agggtgccga tcggctcgcc ggcgaccgcc cggcggatgt
     6601 tcccgtcctc ccacaggttg aagacgatga tcggcaggcg attctccatc gccagcgaga
     6661 tcgcggtggc gtccatcacc tgcagaccgc gcttcagcac ctcgatgtac tcgagggtct
     6721 cgaacttcac cgcgtccggt tgcaggcgcg ggtccgcatc gtacacgccg tccaccgacg
     6781 tccccttcag gatggcttcc gccttgatct ccagcgcccg ctgcgcggcc gtggtgtcgg
     6841 tcgagaagaa cggcgcgccc atgccggcgg cgaagatgac cacccgtccc ttctccaggt
     6901 gccgcatcgc tcggcgcggg atgtagggct cggcgatctg ggccatggcg atggcggtct
     6961 gcacgcgggt gatcacgccg atgttctcga gcgcatcctg cagcgccagc gcgttcatca
     7021 ccgtcgcaag catgccgatg tagtccgccc gcgcccggtc catggactga tggtcgcggc
     7081 cgcgccagat gttgccgccg ccgaccacca ccgcggtctc cacgccgtcg gccaccacct
     7141 ctgccagctg acgcgcgacg ttgccgaggg cctcggagga gatgccgaac tcgcgcccgg
     7201 gcggcgagaa cgcctccccg gagagcttca acaggacccg gcgataggcc ggtcgaagac
     7261 cggctaccgg atcggcgtcc gccaccccgg ccagggctac gcctcctcgc cgagcttgaa
     7321 ccgcgcgaac cggcggacgg cgatcttctc gccgaccttc gccgagaccc gcgccaccag
     7381 gtctcccacc gagacggagt cgtcgcggat gaacggctgg tcgagcagca cggtctgctt
     7441 gaagaacgcc cggagcttgc cctcgacgat cttctcgagg acatgctcgg gcttgccggt
     7501 cgtgcgcgcc tgctccgcgt agatcttccg ctcggcgagg acgacgtcct cggggacgtc
     7561 gtcgcgcgag atccagcgcg gggcgagggc cgcgatgtgc atggccacgt tccgcgccag
     7621 cgtcccgaac tcctcggtgt tggcgacgaa gtcggtctcc gagttcacct ccaccaggac
     7681 gccgagcttg cccggcgggt ggatgtacgc ctcgatcacg ccctcgttcg ccgagcggcc
     7741 ggcgcgcttt cccgccgcgg ccaggccgcg ctcgcgcagg atctcctgcg cgcgagccag
     7801 gtcgccgccc gcgtcctgca gcgcgcgctt gcaatccatc atccccgcgc cggtcagctc
     7861 gcgcaggacg cgaacctcct cggcagtgat cttcgggtcg ctcattccgt cgattctccc
     7921 acaccggtct tccggtccag ggggggtctc tccccggatg tggtccggtc actcgtttcc
     7981 ccgggcgcgc cagccgccac ttcccccgac gactccggga ccacgtcggg ctgtgcctcg
     8041 gggggcacct cggcgccggc ctcgcccgcg gattccggtc cggcccccgc gctgcggtcg
     8101 acacccaccg cgtccggcgg gcccggctcg aagacctgac tctcgggaag ggtcgtcgcc
     8161 ggctcgagct ccatcggctc cggctggaac tccggttcgc cgcgcgcggc gcgagcgatc
     8221 tcctccccgc cgaggaaggc tccctcctcg aaggcgtcgg cgatgatctt cgtcacgagg
     8281 gccaccgacc ggatcgcgtc gtcgttgccc gggatgacgt agtcgacctc gtccgggtca
     8341 cagttcgtgt cgacgatggc gacgaccggg attccgagct tgcgggcctc ggtcaccgcg
     8401 atgtgctcct tcttcgtgtc gatgaccagg atcgcgtcgg ggcggcgggt catggtctgg
     8461 atgccgccca tgttccgctc gagcttctcc ttctcgtggc ggagcttgag cacctccttc
     8521 ttcgggaggt actcgaacgc gccggagcgt tccatgtcct gcagctcgcg cagacgggcc
     8581 aggcgctggc tgatcgtctg gaagttcgtg agcatgccgc cgagccagcg ggtgttgacg
     8641 tagggcatgc cgatccggga cgcctgatcg gccacaacct cctgggcctg cttcttcgtc
     8701 ccgacgaaca ggatcgagcc gcccttccgg gccagctcgc gcacgaacgc gtgggcgcgc
     8761 tgcagcccgg tcagggactg ctccaggtcg atgatgtaga tgccgctgcg ttcggcgaag
     8821 atgaagcgcc gcatcttcgg gttccagcgc cgggtctggt gcccgaagtg gacgcccgcc
     8881 tcgagcagct cccgtcgggt gacgacggac atggccgtgc ctccttccag gttttcgtcc
     8941 tccgcccggt tcatcccctg ccgacccgga gtaccgggac cacggcgcgg gtccccgggc
     9001 gtgcgtattc ggcctctcgg accggtcccc gattctaggg gacgacccac cgaaccgacc
     9061 ggcccgcttc gcccgccggc acgttcgccg gcacccggcg ctcatcggcg gggcggcggc
     9121 gcctcggcca gccggtcgcg caccgccagc cagcggaacg tcagcgcgcc gaggaccccg
     9181 acgtggaaga gcgacccgat cagcgtgaag ccccacgtgg ccaccgacgg cgtgagcggc
     9241 gtggccggcc ctcccgggac gcccgcgaac aggaagaaga cgaacgcgaa gtagagcagg
     9301 atgaaggaca ggtgcccacc gccggggagg cgcgcggcgc ggatgctccg gcggacggcc
     9361 tcgcggtacg gatcgccgtc caccaccgcg gcgatcggcg cgaacccgag caggtagagg
     9421 ccgatcagcg gcgcaccgag ctggacgaac ccggtgaact gtggggcgaa cccctgcagc
     9481 agcaggggga ttgccccggc aacgccgaac agggcgagcg cgacgagcac caggtgcggg
     9541 atcgcccgcc gccaggcgtg caggggccgt tcctcgctcc agccgagatc gggggccatc
     9601 agcgcgatca cgaggccgaa gagg
//
LOCUS       DASPWO010000463         4366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_997292, whole genome shotgun sequence.
ACCESSION   DASPWO010000463 DASPWO010000000
VERSION     DASPWO010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997292"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VEO00_10530"
     CDS             <1..549
                     /locus_tag="VEO00_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58470.1"
                     /translation="FNSDRDGLPMGGFLVDPDGSNLRRIDTDAYVEYPAWSPDGGRIA
                     FMGGDGAAEYDIWVVDVDGTDLVRLTDSFGPDGWPAWSPDGTRIAFTSVRDDCSYSNA
                     PDCRSTGDIGPHHDVWVVNVDGSGLARVTPEFGQFVTWSPDGEYLLVSGYDLYVIRPD
                     GTGRASLELEGLSGGLFPDWIE"
     gene            complement(595..1371)
                     /locus_tag="VEO00_10535"
     CDS             complement(595..1371)
                     /locus_tag="VEO00_10535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYU58471.1"
                     /translation="MLLEGKNAVIYGGGGSIGGAVARAFAREGAKVHLAGRTPERLEE
                     VADEIRSAGGAVETAQVDALDEQAVDAHADAVAAQAGSIDISFNLTSHGDVQGTPLVE
                     MHLEDFERPVRIAVRTQFLTARAAARHMMRQGSGVILTFGGAGGRDPIRDYATGGFQV
                     HLGGTQVAFGAVDVLRRQLACELGPHGIRVVTLQSAGVPETITGEWRETITEGLIAAT
                     MLKRPETLDDIGNVAAFAASDLARTMTATALNVTCGREAD"
     gene            1523..1795
                     /locus_tag="VEO00_10540"
     CDS             1523..1795
                     /locus_tag="VEO00_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HYU58472.1"
                     /translation="MLNKRFTATLQRSPKKGGWTYVVMPDSVEFFGTGGLVKVRGTID
                     GHPFRSSFMAMGDGTHKLPVRADVRTAIGKEAGDTVSVRLQERIED"
     gene            complement(1796..2575)
                     /locus_tag="VEO00_10545"
     CDS             complement(1796..2575)
                     /locus_tag="VEO00_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYU58473.1"
                     /translation="METVTSKDGTAIAFDRSGEGPPVVLVSGGSVDRRSNAPLAERLA
                     SDFTAFNYDRRGRGPSGDTPPYAVQREIEDIDAVIGAAGGSAHLYGSSSGAALALEAA
                     AAGLAITKLALWEPPWSVDESRPRPPGDTAKVFTELVEAGRRDDAVEHFMAKVVGLPP
                     EFVAGARQSPFWGDTVALAHTLAYDATIMGDYVFPRERLGGVTVPTLVLDGGASFGFM
                     GETARLIADTLPNGQHRTLEGQDHNVDPSVLAPALAEFFKA"
     gene            complement(2589..3329)
                     /locus_tag="VEO00_10550"
     CDS             complement(2589..3329)
                     /locus_tag="VEO00_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYU58474.1"
                     /translation="MAHPFEVRKEIEVDATPEEVWEAIATGPGLDAWFMGRNEVEPGE
                     GGSVRMTLPGFSIESSVTVWDPPHRLVTETAKDEHGRVMAFEYVIEGRAGGSTVLRFV
                     HSGFLAGDDWEAEYDALRQGDPAYVQKLAEYLKYFRGRTAVPVSAFGPEVDREPAWTA
                     FKSALGLSGEVAEGEPVRFTVEGVGPIEGVVDYVSRDFLGVRSDDGLYRFIHGFPGSV
                     VLGHHVFADVDPQATERAWQSWLDHLFA"
     gene            complement(3331..3942)
                     /locus_tag="VEO00_10555"
     CDS             complement(3331..3942)
                     /locus_tag="VEO00_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYU58475.1"
                     /translation="MLEVAVIEDAATAEVSLDPVRARLLSELAEPGSATTLAARVGLP
                     RQNVNYHLRALERHGLVELVEERRKGNCTERLLRATAASYVISPAALAAVQPDPARSP
                     DRLSARWLLAVASRLVRDVGALIAGATRARKRAATFALDGEVRFASAADRAAFAADLA
                     SAVAALVAKYHDETAERGRDHRLVVAIHPSVKADEAGRERKEP"
     gene            4157..>4366
                     /locus_tag="VEO00_10560"
     CDS             4157..>4366
                     /locus_tag="VEO00_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58476.1"
                     /translation="MIVRYIDDFDATPRGLLLALDHSTFIGILTNAILGLRLVATAAR
                     RVIWARADQLVFWGLGLMGFVIGLIS"
BASE COUNT          605 a         1575 c         1518 g          668 t
ORIGIN      
        1 ttcaactccg accgagacgg cctgccgatg ggcggcttcc tggtcgaccc ggacggatcg
       61 aacctgcgcc gtatcgatac cgacgcctac gtcgagtacc cggcgtggtc ccccgacggg
      121 ggacggatcg cgttcatggg aggggacggc gcggcggagt acgacatctg ggtcgtcgac
      181 gtcgacggga cggacctggt ccggctgacc gactccttcg gcccggacgg gtggcccgca
      241 tggtcgccgg acggcacccg gatcgcgttc acctcggttc gggacgactg ctcgtactcc
      301 aacgcgcccg actgccggtc caccggcgac atcgggccgc accacgacgt gtgggtggtg
      361 aacgtcgacg gaagcggcct cgccagggtc acccccgagt tcggccagtt cgtgacgtgg
      421 tccccggacg gcgagtacct gctcgtctcg ggctatgacc tctacgtgat ccgtccggac
      481 ggcacgggcc gcgcgtctct cgagctcgag ggcctctcgg gcggcctgtt cccggactgg
      541 atcgagtaga ccgggctgac cccgcgcgct cgagcgccct gtccaggtga ccgctcaatc
      601 ggcctcgcgg ccgcacgtga cgttgagggc ggtcgcggtc atggttcggg ccaggtccga
      661 ggcggcgaag gcggccacgt tgccgatgtc gtcgagcgtc tccggccgct tcagcatggt
      721 cgcggcgatg aggccttcgg tgatggtctc ccgccactcc ccggtgatcg tctcgggcac
      781 gccggcgctc tgcagcgtca cgacgcggat gccgtgaggg ccgagctcgc acgcgagctg
      841 gcgtcgcaac acgtcgaccg cgccgaaggc gacctgggta ccgccgagat ggacctggaa
      901 ccccccggtg gcgtagtcgc ggatcgggtc gcggccaccg gcgccgccga aggtgaggat
      961 gacccccgat ccctgcctca tcatgtgacg cgcggccgcc ctcgcggtca ggaactgtgt
     1021 cctcacggcg atcctgaccg gccgctcgaa gtcctccagg tgcatctcga cgagcggcgt
     1081 tccctggacg tcaccatgag aggtcaggtt gaaggagatg tcgatgcttc cggcttgcgc
     1141 cgccacggcg tcggcgtgcg catcgacggc ctgctcgtcg agcgcatcga cctgtgccgt
     1201 ctccaccgct cctcccgccg agcggatctc gtcggccacc tcctcgaggc gctccggggt
     1261 tcgaccggcg agatggacct tcgccccctc gcgcgcgaag gcgcgggcca ccgctccgcc
     1321 gatcgagccg cctcctccat agatgaccgc gttcttgccc tcgagcagca cgtcacgcct
     1381 cctccctctc gcggttcccc tcccagcttg ccccggagcg acctcgggcg tgacgctcat
     1441 cgtgatcggg ggccgcactc cgtcttcggg gcggccgcga tcctggttcg ggcagatcgg
     1501 atgctcttgg tagggtgcgg gcatgctgaa caagcggttc accgccaccc tgcaacggag
     1561 tcccaagaag ggcggctgga cctacgtggt catgcccgat tcggtcgagt tcttcgggac
     1621 cggggggctc gtcaaggtcc gaggcaccat cgacggccac ccatttcgca gttcgttcat
     1681 ggcgatgggc gacggcacgc acaagctgcc cgtcagggcg gacgtccgca cggcgatcgg
     1741 gaaggaagcg ggcgataccg tgagcgttcg cctgcaggag cggatcgaag actgatcacg
     1801 ctttgaagaa ctccgccagg gccggagcca ggaccgaagg gtccacgttg tggtcctgcc
     1861 cttcgagcgt ccggtgctgc ccgttgggga gcgtgtccgc gatcagccgg gcggtctcgc
     1921 ccatgaaccc gaagctggcg ccgccgtcca gcacgagggt gggcaccgtg acgccgccca
     1981 ggcgctcgcg cgggaagacg tagtcgccca tgatcgtggc gtcataggcg agggtgtgtg
     2041 ccagggcaac cgtgtcaccc cagaacggcg actggcgcgc gccggcgacg aactccggcg
     2101 gaaggccgac gaccttcgcc atgaagtgct ccaccgcgtc gtcgcggcgg cccgcctcga
     2161 cgagctcggt gaacaccttc gccgtgtcgc ccggggggcg tggccggctc tcgtcgacgc
     2221 tccacggcgg ctcccacagc gcgagcttcg tgatcgccag tcctgcggcg gcggcctcga
     2281 gcgccagggc cgcgcccgac gacgagccgt acaggtgcgc cgagccgccg gcggcaccga
     2341 tgacggcgtc gatgtcctcg atctcccgct ggaccgcgta cggaggcgtg tcgccgctcg
     2401 ggccccgccc gcggcggtcg tagttgaacg ccgtgaagtc cgaggcgagc cgctccgcca
     2461 gcggcgcgtt cgagcggcgg tcgaccgagc ccccgctcac caggacgacc ggcggtccct
     2521 cgcccgaccg gtcgaacgcg atcgccgtac cgtccttcga ggtgaccgtt tccatgcgac
     2581 ctcctcgctc aggcgaacag atggtccagc caggactgcc aggcgcgctc ggtcgcctgc
     2641 gggtccacgt cggcgaagac gtggtgtccg aggacgacac taccggggaa cccgtggatg
     2701 aaccggtaca gcccgtcgtc ggagcgcacg cccaggaagt cccgcgacac gtagtcgacg
     2761 acgccctcga tcggcccgac gccctccacg gtgaaccgca ccggctcgcc ctcggccacc
     2821 tcgccggaga ggccgagcgc ggacttgaag gccgtccagg ccggttcccg gtcgacctcg
     2881 ggcccgaagg cggagacggg caccgcggtg cggccgcgga agtacttcag gtactccgcg
     2941 agcttctgca cgtacgcggg gtcgccctgc cgcagcgcgt cgtattcggc ttcccagtcg
     3001 tcgccggcga ggaacccgct gtgcacgaag cggagcaccg tgctgccgcc ggcgcggccc
     3061 tcgatcacgt actcgaacgc catcacccgc ccgtgctcgt ccttcgcggt ctcggtcacg
     3121 agacggtgcg gcgggtccca gacggtcacg ctggattcga tcgagaatcc gggaagggtc
     3181 atccgcactg agccgccctc tccgggctcg acctcgttcc gtcccatgaa ccaggcgtcg
     3241 agcccagggc cggtggcgat cgcctcccag acctcttccg gcgtcgcgtc gacctcgatc
     3301 tctttacgca cctcgaaggg gtgggccatc tcagggctcc ttcctctccc gtccggcctc
     3361 gtcggccttg acgctcgggt ggatggcgac gaccaggcgg tgatcgcgtc cgcgctccgc
     3421 cgtctcgtcg tggtacttcg cgaccagggc cgcgaccgcg ctcgccaggt ccgcggcgaa
     3481 ggcggcgcga tcggcggccg aggcgaagcg cacctcgccg tcgagggcga acgtcgccgc
     3541 ccgcttgcgt gcccgggtcg cgccggcgat cagcgcgccg acgtcgcgca cgagcctgga
     3601 ggcgaccgcc agcagccagc gcgcagagag ccgatcgggc gagcgggcgg ggtcgggctg
     3661 cacggcggcc agcgcggcgg gggagatcac gtaggacgcg gccgtggcgc ggagcaggcg
     3721 ctcggtgcag ttccccttcc ggcgctcctc gaccagttcg accagcccgt gccgctccag
     3781 cgcccggagg tggtagttca cgttctgcct cgggaggccg acgcgagcgg ccagggtggt
     3841 ggccgagccc ggctccgcca gctccgacag cagccgggcc cgcaccgggt cgagcgacac
     3901 ctcggccgtc gccgcgtcct cgatcaccgc cacttcgagc atacgaggat cctgccaccg
     3961 acaactttct ttgtcaagat gagcaggttc gtcggtgcgg tcgccgtccc tccgcgcgct
     4021 tgacatcggg acacggccgt cgaagggttc acgcgcatca cgttcgagat cgaggcgatg
     4081 agcggcggca tcaccggcgc gaaacgcggc cgcatccctc ctgtggctga tcccggccct
     4141 cgggctgctc gcgtacgtga tcgtccgcta catcgacgac ttcgacgcca cgccccgggg
     4201 cctgctgctc gccctggacc actcgacgtt catcgggatc ctgacgaacg cgatcctcgg
     4261 gctccggctc gtggcgaccg ccgcgcggcg ggtcatctgg gcccgggccg accagctcgt
     4321 gttctggggc ctgggactca tggggttcgt gatcgggctg atctcg
//
LOCUS       DASPWO010000464         5772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_407780, whole genome shotgun sequence.
ACCESSION   DASPWO010000464 DASPWO010000000
VERSION     DASPWO010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407780"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="VEO00_10565"
     CDS             <1..1445
                     /locus_tag="VEO00_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58477.1"
                     /translation="LADRLWTVNDEYSRGKIYYDSFGAKRILTWFASGELFDRGRPPM
                     ITILVAVGILAALWRYRRDERARVLLFLFLVSLVLFFGRPTLGPVLRLLPGSGDLFLR
                     RFVFGVHLAGLYLAGFGAVTLGRIARDRLQRFRALRERPVAITALAVAGVAVFLAPGF
                     IERTAWASQGAEWIGKQAVADDRGGADVAALVDIARARGPGRFYAGMRSNWGSAYEVG
                     QVPVYVVLLNEGTEDVGFVRPTWSLSSPFEYRFTDTNPSHYDLFDVRYLILDARKTPP
                     VPAERVAERGRHVLWERPDAGYVDLVDVLPPITADRTDLGLRGEDWFRSDLPARGANP
                     GVAFEGEPAPEATVRADELPDTPAGTVVSEDVDLRDGRATATVVADRRAMVMLKASFD
                     PRWQVTVDGIAVSPQMIAPSFVGRLVAPGRHVVRFVYEPFPRYDLLLTFGALTLLGLA
                     LLPGWFARRRPDGSSHVTHEPGAEPSGAGT"
     gene            1716..2447
                     /gene="rplJ"
                     /locus_tag="VEO00_10570"
     CDS             1716..2447
                     /gene="rplJ"
                     /locus_tag="VEO00_10570"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HYU58478.1"
                     /translation="MPNAEKIAKVAALKERIRDAEALLLTEYRGLSVHDATELRRALS
                     EQARFAIVKNTLMVRAAADAGVAELEVLLDGPTAVAFVQGDVVATAKKVVDAAKKFPA
                     LVIKGGYLDGKVLSAAEAQSLATLESREVMLSKVAGMLKAEMTRAASMLQAIQGRFLG
                     VLEAYKEKLPGEVTDEPAEAEPAAEAESAAEAETVEAEPAAEAEPAADTTTDDEPDKP
                     QPEPEGEPHSQPEGEPEAEPEGEKE"
     gene            2451..2846
                     /gene="rplL"
                     /locus_tag="VEO00_10575"
     CDS             2451..2846
                     /gene="rplL"
                     /locus_tag="VEO00_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HYU58479.1"
                     /translation="MAKTKMTADDLLDQFKDMTLLELSDFVKKFEETFDVQAAAAGPM
                     MMAAPGAPGGAAEEVEEQTEFDVVLTGAGDKKIQVIKEVRALTSLGLKEAKDLVDSAP
                     KPVLEKVNKEAADKAKEQLEGAGAAVEIK"
     gene            2985..3404
                     /locus_tag="VEO00_10580"
     CDS             2985..3404
                     /locus_tag="VEO00_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYU58480.1"
                     /translation="MTDERTFGQRLRATRIKAGYSQSDLEEISGIPKARLSRYENGHV
                     APSIQTLERLARALEVSEASLLGDDRALMEEFFHVLTTRGVRIGSSEQAVALGSAVAD
                     IARALGMVEDGEGAVANRSTLSPVGVTGTATWSPDGS"
     gene            3589..>5772
                     /locus_tag="VEO00_10585"
     CDS             3589..>5772
                     /locus_tag="VEO00_10585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HYU58481.1"
                     /translation="MPDSLVPVRDRVSFSKLDEALPLPDLVGIQRESFDWLLAEGLKE
                     VLEEVSPIEDFTEQFQLHFGKHAFKEQKFTEEECKDKDATFSAPLFVEAMFVNKTTGE
                     IKEQEVFMGDFPMMTERGTFIINGTERVVVSQLVRSPGVYFSRELDKTTDKDVYIAKI
                     IPSRGAWLEFDVDKKDTVGVRIDRKRRQNVTVLLKALGWSADEILQLFDHAESIKNTL
                     EKDHVETPEEALEDIYRKLRPGEPPTAESARTLLENLFFNHKRYDLARVGRYKVNKKL
                     EAAQGVLQTQLKARARQLAELDNPDKKGWDQPKFRVWSELQKDEQGENGPKYKGILSY
                     EDMLRTVRYLVKLHAGEEGYEPDDIDHFGNRRLRSVGELIQNQIRIGLSRMERVVRER
                     MTTQDVEAITPQTLINIRPVVASIKEFFGTSQLSQFMDQTNPLAGLTHKRRLSALGPG
                     GLSRERAGFEVRDVHPSHYGRMCPIETPEGPNIGLIGSLSTYGRINPFGFIETPYRRV
                     TGGKVTDKVDYLSADEEDRHVIAQANEPIKPDGGYANPTVLVRRKGGEVDSVVPDEVD
                     YMDVSPKQIISVAAALIPFLEHDDANRALMGANMQRQAVPLLRSEAPIVGTGVEFRAA
                     VDAGDVVLAEEAGVVEDVSADDIVIRERTGSLKTYKLQKFRRSNQGTSLNQKPLVNEG
                     DKVVRNQVIADGPSTEAGELALGRNLIVAYMPWEGHNYEDAIIISE"
BASE COUNT          976 a         1933 c         2001 g          862 t
ORIGIN      
        1 tgctcgccga ccggctgtgg acggtgaacg acgagtactc gcgcgggaag atctactacg
       61 actcgttcgg ggcgaagcgg atcctgacgt ggttcgcgag cggcgagctg ttcgaccgtg
      121 ggcgaccgcc gatgatcacg atcctggtcg cggtgggcat cctggcagcg ctctggcggt
      181 accgtcgcga cgagcgggcc cgcgtgctcc tgttcctgtt cctcgtttcg ctggtcctct
      241 tctttgggcg gccgacgctc ggacccgtcc tgcggctgtt gcccggctcc ggcgacctgt
      301 tccttcggcg gttcgtgttc ggggtgcacc tggcggggtt gtacctcgcc gggttcggcg
      361 cggtcacgct cggccggatc gcgcgggacc ggctgcaacg attccgagca ctgcgagaac
      421 gacccgtggc gatcacggcg ctggcggtcg ccggtgtcgc ggtcttcctc gcccccggct
      481 tcatcgaacg cacggcgtgg gcctcgcagg gggccgaatg gatcgggaag caggcggtcg
      541 ccgacgaccg gggcggcgcc gacgtcgccg ccctggtcga catcgcgcga gcacgagggc
      601 cgggtcggtt ctacgcgggc atgcggtcca actggggctc ggcctacgag gtcggtcagg
      661 tacccgtgta cgtggtgctc ctgaacgagg gcacggagga cgtcggcttc gtccggccga
      721 cctggtccct ctcatcaccg ttcgagtacc ggttcaccga cacgaacccg tcgcactacg
      781 acctgttcga cgtccgatac ctgatcctcg acgcgagaaa aacgcccccc gtccctgcgg
      841 agagggtcgc cgaacgcggg cgccacgtgc tctgggaaag gcccgacgcc ggctacgtcg
      901 acctcgtcga cgtcctgcca ccgatcacgg ccgaccggac ggacctcggg ctgcgcgggg
      961 aggactggtt ccgttcggac ctcccggcca ggggcgcgaa cccgggcgtc gcgttcgagg
     1021 gggagccggc gcccgaagcc accgtgaggg ccgacgaact cccggacacg ccggccggca
     1081 ccgtcgtcag cgaggacgtt gaccttcgcg acgggagggc caccgcgacg gtggtcgccg
     1141 accggcgggc gatggtgatg ctgaaggcgt cgttcgaccc ccgttggcag gtgaccgtgg
     1201 atggcatcgc agtgtcgccg cagatgatcg cgcccagctt cgtcgggcgg ctcgtggcgc
     1261 ccggccggca cgtcgtccgg ttcgtgtacg agccgttccc gaggtacgac ctcctgctga
     1321 cgttcggggc gctgacgctg ctgggcctcg ctctgctccc gggttggttc gcgcgccgcc
     1381 gcccggatgg gtccagtcac gtcacgcacg agcccggtgc cgagccgagt ggtgcgggga
     1441 cctagaggac ccctcctacc cggccgtcgc gagctcgcct cgtctccttc tgcggacccg
     1501 cctgctgaac ccggatgacc gcgagcggct ccttgtcgcc gactccgttg tcgcccgagc
     1561 cggtggctcg gtaccctttg gcttcgccgc agacagcagg ggtgcgcagg cacgtaatcc
     1621 cccgggggca cctgccgagg cgacgaagac gcatcgctct cgagcgcgtc tccgctgtct
     1681 gcggggacgc gtttcccgtt ttccctggag ggtcgatgcc gaacgccgag aagatcgcga
     1741 aggtcgccgc gctcaaggag cgcatccgag acgccgaggc gctgctgctg accgagtacc
     1801 gcggtctctc ggtgcacgac gcgaccgagc tccgccgggc cctgtccgag caggcgcggt
     1861 tcgcgatcgt caagaacacc ttgatggtgc gcgcggccgc cgatgcgggc gtcgccgagc
     1921 tcgaggtgct cctggacggc cccaccgcgg tcgcgttcgt ccagggcgac gtggtcgcga
     1981 ccgccaagaa ggtcgtggac gccgcgaaga agttcccggc cctcgtgatc aagggcggct
     2041 acctggacgg caaggtgctc tcggccgccg aggctcagtc cctcgcgacc ctcgagtcga
     2101 gagaggtgat gctctcgaag gtcgccggga tgttgaaggc cgagatgact cgggcggcct
     2161 cgatgctcca ggcgatccag ggtcggttcc tcggagtgct cgaggcctac aaggagaagc
     2221 tccccggcga ggtgaccgac gagcccgccg aggccgagcc cgccgccgag gcggagtcgg
     2281 ccgcagaagc cgagaccgtc gaggccgagc ccgccgccga ggccgagccc gccgccgaca
     2341 cgacgaccga cgacgagcca gacaagccgc aaccagagcc agagggagag ccgcacagcc
     2401 agccagaggg agaacccgag gcagagcccg agggagagaa ggagtagagg atggccaaga
     2461 ccaagatgac cgccgacgac ctgctcgatc agttcaagga catgacgctg ctcgagctgt
     2521 cggacttcgt caagaagttc gaagagacct tcgacgtcca ggccgccgcg gccgggccga
     2581 tgatgatggc cgcgcccgga gccccgggtg gcgctgccga agaggtcgag gagcagaccg
     2641 agttcgacgt ggtcctcacc ggcgccggcg acaagaagat ccaggtgatc aaggaggtgc
     2701 gggccctgac gagcctcggc ctcaaggaag ccaaggacct cgtggactcc gcgccgaagc
     2761 cggttctgga gaaggtcaac aaggaagcgg ccgacaaggc caaggagcag ctcgagggcg
     2821 ccggcgccgc cgtcgagatc aagtaggccg aacggccgag gaggggacca tcgccagatt
     2881 cgcaaggcct cgccatcgcc aggttccgaa gctccgcgtt gcggtaaggc tccgcatggg
     2941 gatagattcc ccgtcgagca accaccgtcg atagggggcg ttgcatgacc gacgaacgca
     3001 cgttcggaca gaggctccga gcgacccgga tcaaggcggg gtactcgcag tcggacctgg
     3061 aggagatctc cgggatcccg aaggcgcgcc tgtcgcggta cgagaacggg cacgtcgctc
     3121 cttcgatcca gacgctcgag cgcctcgccc gggcgctgga ggtgtcggaa gcttcgctcc
     3181 tgggggacga ccgcgcgctc atggaggagt tcttccacgt cctcaccacg cgcggcgtcc
     3241 ggatcggctc gagcgagcag gcggtcgcgc tcgggagcgc cgtcgccgac atcgcgcgtg
     3301 cactcgggat ggtggaggac ggggagggcg ccgtcgcgaa ccggtcgacc ctctcgccgg
     3361 tcggtgtgac gggcaccgcc acctggtcac ccgacggcag ctaggctctc ctttgatggg
     3421 ttcccaaggg ttgacctccg ctccgagtgg gggtaacctt cggccgattt cgaccgggtc
     3481 cccgcccggg ggttgacgct gcgcgcccgc gcacgtagac tgctcgtttg ccgtgccgcg
     3541 ttccgcatgc cctggatcca gggttctcta ccctaggggg gatcgttctt gcctgactcg
     3601 ctcgtccctg tccgcgaccg tgtcagtttc tccaagctcg acgaggcgct accgcttccc
     3661 gacctcgtcg gcatccaacg ggagtccttc gactggctcc tggccgaggg tctgaaggag
     3721 gttctcgagg aagtctctcc gatcgaggac ttcaccgaac agttccaact gcatttcggg
     3781 aagcacgcct tcaaggaaca gaagttcacc gaggaggagt gcaaggacaa ggacgccacc
     3841 ttctccgctc cgctgttcgt cgaggccatg ttcgtcaaca agacgaccgg cgagatcaag
     3901 gagcaggagg tcttcatggg ggacttcccc atgatgaccg agcgcggcac cttcatcatc
     3961 aacggcaccg agcgggtggt cgtgtcccag ctcgtgcggt ccccgggggt ctacttcagc
     4021 cgcgagctgg acaagaccac cgacaaggac gtctacatcg ccaagatcat ccccagccga
     4081 ggggcgtggc tcgagttcga cgtcgacaag aaggacaccg tcggtgtccg gatcgaccgc
     4141 aagcgccggc agaacgtcac ggtgctgctc aaggcgctcg ggtggtcggc ggacgagatc
     4201 ctccagctct tcgaccacgc cgagtcgatc aagaacaccc tcgagaagga ccacgtcgag
     4261 acccccgagg aggccctgga ggacatctac cgcaagctgc ggcccggtga gccgccgacg
     4321 gccgagtccg cccggacgct ccttgagaac ctcttcttca accacaagcg ctacgacctg
     4381 gctcgggtgg gtcgctacaa ggtcaacaag aagctcgagg ccgcacaggg cgtgctccag
     4441 acacagctga aggcccgtgc caggcagctg gccgagctcg acaaccccga caagaagggg
     4501 tgggaccagc cgaagttccg cgtctggtcg gagctccaga aggacgagca gggcgagaac
     4561 gggccgaagt acaaggggat cctgtcctac gaggacatgc tccgcacggt gcgatacctc
     4621 gtgaagctgc acgcgggcga ggaagggtac gagcccgacg acatcgacca cttcgggaac
     4681 cggcgcctgc gctcggtcgg cgagctgatc cagaaccaga tccggatcgg tctgtcgcgc
     4741 atggagcggg tcgtccgcga gcggatgacg acgcaggacg tcgaggcgat cacgccccag
     4801 acgctgatca acatccgtcc ggtggtcgcg agcatcaagg agttcttcgg cacctcgcag
     4861 ctgtcgcagt tcatggacca gaccaacccg ctcgccgggc tgacccacaa gcgccggctg
     4921 tcggcgctag ggccgggcgg gctctcgcgc gagcgcgcgg ggttcgaggt ccgggatgtg
     4981 cacccctccc actatggccg catgtgcccg atcgagacgc cagaaggtcc gaacatcggt
     5041 ctgatcgggt cgctctcgac gtacgggcgc atcaacccct tcgggttcat cgagaccccg
     5101 taccgccggg tgacgggcgg caaggtcacc gacaaggtcg actacctctc ggccgacgag
     5161 gaggaccggc acgtcatcgc gcaagccaac gagccgatca agcccgacgg tggctacgcc
     5221 aaccccacgg tcctggtccg tcgcaagggc ggcgaggtcg actcggtcgt gcccgacgag
     5281 gtcgactaca tggatgtctc gccgaagcag atcatctcgg ttgctgcggc cctgatcccg
     5341 ttcctcgagc acgacgacgc caaccgcgcc ctcatggggg ccaacatgca gcggcaggcc
     5401 gtgcccctgc tccggtcgga ggccccgatc gtcgggacgg gcgtggagtt ccgcgccgcc
     5461 gtcgacgccg gggacgtggt cctggccgag gaggccgggg tcgtcgagga cgtctcggcc
     5521 gacgacatcg tgatccgcga gcggaccggc tcgctgaaga cctacaagct ccagaagttc
     5581 cgccggtcca accagggcac gtcgctcaat cagaagccgc tggtcaacga aggcgacaag
     5641 gtcgtccgca accaggtgat cgccgacggg ccgtccacgg aggcgggcga gctcgccctc
     5701 ggccgcaacc tgatcgtggc gtacatgccg tgggagggcc acaactacga ggacgcgatc
     5761 atcatctcgg ag
//
LOCUS       DASPWO010000465         6294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1250866, whole genome shotgun sequence.
ACCESSION   DASPWO010000465 DASPWO010000000
VERSION     DASPWO010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250866"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VEO00_10590"
     CDS             <1..311
                     /locus_tag="VEO00_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58482.1"
                     /translation="RFGSSFDWRLAIKYPGDTDWAVARIAEDMWDKKGWAIGEVSKYG
                     AGASADNSEFNLQYRNLNDQWGPWKDVGCWSDSLADWSANRTSGTEFKVVQQEGYCFD
                     "
     gene            934..1134
                     /locus_tag="VEO00_10595"
     CDS             934..1134
                     /locus_tag="VEO00_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HYU58483.1"
                     /translation="MPQEQERKQTQRKSGGAGEGDAGQVDASKKGTELKEELDDILDE
                     IDSVLEENAEEFVKSYVQKGGE"
     gene            1131..1973
                     /gene="prcB"
                     /locus_tag="VEO00_10600"
     CDS             1131..1973
                     /gene="prcB"
                     /locus_tag="VEO00_10600"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HYU58484.1"
                     /translation="MTNPFDSSGYPMGANPSFADVLRRAGQDAAVPRPVEPGALPAHP
                     HGTTVLALSFADGLVMAGDRRATEGYSIADERTEKVFPADAHSLVSIAGAAGQAVEMV
                     RLFQTELEHYEKVEGESLSLEGKANRLAQMIRGNFGWALQGLIVVPLFGGYDLRRGEG
                     RLFRYDVTGGRWEEFDHHATGSGGQTAKATLKKRWRPGLSRDEAVRAAVEALYDASQE
                     DIATGGIDPIRGIFPTVKVAAAGGVTDVTEDEVRAAFEAIVAERVGTEPLGRTTPGQA
                     EAER"
     gene            1977..2669
                     /gene="prcA"
                     /locus_tag="VEO00_10605"
     CDS             1977..2669
                     /gene="prcA"
                     /locus_tag="VEO00_10605"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HYU58485.1"
                     /translation="MMSFQPYVPPEQAMKDRSDYARKGIARGKSVAAMEFADGIAFLA
                     ENPSATLHKISEIYDRISFAGVGKYSEFDSLRIAGIQLADVRGYSYGREDVTAKSLAN
                     AYSQALSNIFTQQWKPFEVEILVAEVGNTPGENALFHILFDGSVNDEQGYVAMGGRSE
                     EIRAQLKDRYREGLSADEALRFCIEAIQSVEERTVPPEDLEAGILDRTLPRRKFRRLT
                     DDQVAALAPAGG"
     gene            2760..3971
                     /locus_tag="VEO00_10610"
     CDS             2760..3971
                     /locus_tag="VEO00_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58486.1"
                     /translation="MLPVVLGIVLAISLIANLLLAGSLRQANRRADRLTQRPATPIAS
                     GEPSPAGGGGELAAVREIQDQTATMRELAFTRDLEPEFLSDEELARDLQTAIPDEDLE
                     AGLPDEEVLKALGMLPSGDDLVDTLRAAAEANVLGYYDPETERLVVRSGQEALTPLGK
                     ITLSHELTHAVTDQHYDLERVLTGEEPDDVAIGTLALIEGDATLEMNLWAQQYLSVEE
                     QVQLGLEAATGLLSGEGQLPPGLEALQSFPYLDGMAFVQYLYEQNGWKAVNDAYGDPP
                     STSEQVLHPEKYLSGETGERVTVEAPIPGSTRFDRGEVGELFLRTLLDVSGVGNAALA
                     ADGWDGGAYEALRGPDGVIVAFALRWDTSADDDAFEDALAGASIGGCVDLAPGGPTDT
                     RFTVYPGTCRA"
     gene            4159..5217
                     /locus_tag="VEO00_10615"
     CDS             4159..5217
                     /locus_tag="VEO00_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58487.1"
                     /translation="MLNHVLPGLRRDAADVRELPYERTVVVRLLPSVEAIPDVFRVLD
                     VGGDPLPSRPVLSALDMIGPLPGSGELSGYYDPGTGVVTIFDNVDLDRPYGRGVVIHE
                     LTHALVDQRFDLDLVGRAIVDGDSFLATSSLVEGDAMLAEHLWLERTAPEEPSPFHGY
                     RDPEDGEALFAGFPYVRGYEFVRSLYDAGGWEALNEAYRRPPVTSEQVLHPEAYLAGD
                     SGVPAPEEVMSPAPGLGAQFEDGRVGELFLRGLLGPTAPASASEGWDGGAYHAFRDDE
                     GRVSVHVVLAWDAPSDVGEFVGALGRWAAEAFGGFTDFGPRETGHRYGAGSHCIDMAV
                     DEFHALVTFTVIGHPCDA"
     gene            5253..>6294
                     /gene="pafA"
                     /locus_tag="VEO00_10620"
     CDS             5253..>6294
                     /gene="pafA"
                     /locus_tag="VEO00_10620"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HYU58488.1"
                     /translation="MDRRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVHWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDSVRDLVAHDKAGERILEALLGAAEVRLHEEGI
                     SGDVYLFKNNTDSAGNSYGCHENYLVARQGEFARMADVLIPFFVTRQIWCGAGKVLHG
                     PRGAQFCIAQRAEHIWEGVSSATTRSRPIINTRDEPHADAERFRRLHVIVGDSNMSEF
                     TSWLKVGVTDLVLRMVEENAVMRDLTLENPIRAIREISHDMTCRKKVRLATGRELSAI
                     EIQAEYLERAARFLDRHGADAHLGEVLEQWRFALQALAEGALERLGRQVDWVAKYLLI
                     ERYRATHNMGLAS"
BASE COUNT          951 a         2184 c         2237 g          922 t
ORIGIN      
        1 aacgcttcgg aagttctttc gactggcgcc tggcgatcaa gtatccgggt gataccgact
       61 gggccgtcgc gaggatcgcc gaggacatgt gggacaagaa ggggtgggcg atcggagagg
      121 tatccaagta cggtgccggc gcctccgcgg ataacagcga gttcaatctc cagtaccgaa
      181 acctgaacga ccaatggggg ccgtggaagg atgtgggctg ctggtccgac agtctcgcgg
      241 actggagcgc gaatcggact tccggaacgg aattcaaggt tgtgcagcag gaggggtatt
      301 gcttcgacta gccgttccaa gcgctcgccg gtgctccttc gcgctgctga ccagcggagt
      361 cctcttcttg ctggtcgcct gcggtcgatt cgagggggat gatgtaacga ctccggaggc
      421 cgagcttccc gagttcatgt ggattcagga tggtgtttcg ttcgacatca ccggcccgtc
      481 gatatcgccg gctcaggtac ccgtcctcgg cgcacagttg ttcctatctg cgtcgtagcc
      541 catgtcccga agggccgccc gactgcccgc aatcgcgatc ctccttgcct cgctcgcgct
      601 ggccatcgcc atcccgagcg aacgaatccg atgcggtgcc cctacgccgt catccgcacc
      661 ggaggagcag aatctgcccg ctccctgcca aggggtcccg gaccgcgtgg atcacttgat
      721 ccgcctgcgc gccaccatcc tcgcggtcgg gctcgggggc gcgctcgtgg tcctgttcgt
      781 cccctggccg acgatccgat cgagacgccc tcatcgaacg gcagtccccc cggtaacgac
      841 cggctctcgg cctgaagccg agcgggtacg gttccgtcat ccccggcggg cggtatcctg
      901 tcctctcccc cgaatccatg aggaggtcgc gcgttgccgc aggagcagga gcgcaagcag
      961 acccagcgca agtcgggcgg cgccggcgag ggcgacgccg ggcaggtcga cgcctcgaag
     1021 aagggcaccg agctgaaaga ggagctcgac gacatcctcg acgagatcga ctcggtgctg
     1081 gaggagaacg ccgaggagtt cgtgaagtcc tacgtgcaga agggcgggga gtgacgaacc
     1141 cgttcgactc ctccggctac ccgatggggg ccaacccgag cttcgccgac gtcctgcgcc
     1201 gcgcaggcca ggacgccgcg gttccccggc cggtcgagcc cggtgccttg cccgcgcacc
     1261 cgcacgggac caccgtcctg gccctctcct tcgccgacgg gctcgtcatg gccggcgacc
     1321 ggcgcgccac ggagggctac tcgatcgccg acgagcgcac cgagaaggtc ttcccggccg
     1381 atgcccactc cctggtctcg atcgccgggg ccgccggcca ggccgtcgag atggtccggc
     1441 tgttccagac cgagctcgag cattacgaga aggtcgaggg ggaatcgctc tccctggagg
     1501 gcaaggcgaa ccggctggcg cagatgatcc gcggcaactt cggctgggcc ctgcagggcc
     1561 tgatcgtggt gccgctcttc ggcgggtacg acctgcgccg tggggagggc cgcctgttcc
     1621 gctacgacgt cacgggtggc cgctgggagg agttcgacca ccacgccacg ggctccggcg
     1681 gccagacggc gaaggccacc ctgaagaagc gctggcgccc cggcctctcc cgcgacgagg
     1741 cggtccgcgc ggccgtggag gccctgtacg acgcctccca ggaggacatc gccaccgggg
     1801 gcatcgaccc gatccgcggc atcttcccca cggtgaaggt ggccgccgcc ggcggcgtca
     1861 cggacgtgac cgaggacgag gtccgggcag ccttcgaggc gatcgtcgcc gagcgcgtcg
     1921 gcaccgagcc gctcgggagg accacgcccg gtcaggccga ggcggagcgc tgagcgatga
     1981 tgtccttcca gccctacgtc ccgccggagc aggcgatgaa ggaccgctcc gactatgcgc
     2041 gcaagggcat cgcgcgcggg aagtccgtgg cggcgatgga gttcgccgac ggcatcgcgt
     2101 tcctggccga gaaccccagc gccacgctgc acaagatcag cgagatctac gaccggatct
     2161 ccttcgccgg cgtcgggaag tacagcgagt tcgactcgct gcgcatcgcc ggcatccagc
     2221 tggccgacgt gcgcggctac tcctacggcc gggaggacgt caccgcgaag tccctggcca
     2281 atgcctactc gcaggcactg tcgaacatct tcacccagca gtggaagccg ttcgaggtcg
     2341 agatcctggt ggccgaggtc gggaacacgc cgggcgagaa cgcgctcttc cacatcctgt
     2401 tcgacggctc ggtgaacgac gagcagggtt atgtggcgat gggcgggcga tcggaggaga
     2461 tccgcgccca gctgaaggac cgctaccgcg agggcctctc ggccgacgag gccctgcgct
     2521 tctgcatcga agccatccag tcggtcgagg agcggaccgt gccgccggag gacctggagg
     2581 cggggatcct cgaccgcacg ctgccgcgcc ggaagttccg ccggctgacc gacgatcagg
     2641 tcgcggcgct ggcgcccgcg ggggggtagg tgcagacccc ggccccgccg gcccccgacc
     2701 tgccgcgaca ccggactcca cacgatgcgg accgcgagcg gaccgtcgag cgatcgtccg
     2761 tgctcccggt ggtcctgggc atcgtgctcg ccatcagcct gatcgcgaac ctgttgctgg
     2821 ccggcagcct gcggcaggcc aaccggcggg cggaccgcct cacgcagcgg ccggccaccc
     2881 ctatcgcgtc gggcgagccg agccccgcgg gcggcggcgg ggagctggcc gcggtgcgcg
     2941 agatccagga ccagacggcg accatgcgcg agctggcgtt cacccgcgac ctggagccgg
     3001 agttcctgag cgacgaggag ctggcccggg acctgcagac cgcgatcccg gacgaggacc
     3061 tcgaggccgg gctgcccgac gaggaggtcc tgaaggcgct ggggatgttg ccgtcgggcg
     3121 acgacctagt ggacacgctg cgcgccgcgg ccgaggcgaa cgtcctcggg tactacgacc
     3181 cggagaccga gcggctggtg gtccgatcgg gccaggaggc gctgacgccg ctcggcaaga
     3241 tcacgctctc gcacgagctg acgcacgccg tcaccgacca gcactacgac ctggagcgcg
     3301 tgctgaccgg cgaggagccg gacgacgtgg cgatcggaac gcttgcgctg atcgagggtg
     3361 acgccacgct ggagatgaac ctctgggccc agcagtacct ctcggtcgag gaacaggtcc
     3421 agctggggct cgaggcggcc accggcctcc tgtcggggga gggccagctg ccgccggggc
     3481 tcgaggcgct gcagtcgttc ccatacctcg atggaatggc gttcgtacag tacctgtacg
     3541 aacagaacgg ctggaaagcc gtgaacgacg cgtacggcga cccgccgtcc accagcgagc
     3601 aggtcctgca ccccgagaag tacctctccg gcgagacggg ggagcgggtg accgtggagg
     3661 cgccgatccc cggctccacc cggttcgacc gcggcgaggt cggggagcta ttcctgcgga
     3721 cgctgctcga cgtctccggc gtcggcaacg cggccctcgc cgccgacgga tgggatggtg
     3781 gcgcctacga ggccctgcgc gggccggacg gcgtgatcgt ggccttcgcc ctgcgctggg
     3841 acaccagcgc cgacgacgac gccttcgagg acgccctggc cggcgcgtcg atcggcggct
     3901 gcgtggacct cgcccccggt ggccccaccg acacccggtt cacggtctac cccggcacct
     3961 gccgggcctg agcggtccgt ggtcgagccc ccgccgactc cggtcctgcc accgcctcct
     4021 ccgccacgcc ggcggcgacg gtcgtggccc tgggccgccg gcgcgatcgc gctgacgatc
     4081 gcgctgatcg cggcgctcgg cgcggtcggt gcgtcgaaac agtcgcaccg gagggacgag
     4141 atcagagcgg atcgcgcgtt gctgaaccat gtgctccccg ggctgcggcg cgacgccgcg
     4201 gacgtccggg agcttcccta cgagcggacc gtggtcgtgc gcctgctccc gagcgtcgaa
     4261 gcgatcccgg acgtgttccg cgtgctggac gtcggcggcg atccgctgcc gagccggccg
     4321 gtcttgagcg cgctggacat gatcggcccc ctcccggggt ccggggagct gagcgggtac
     4381 tacgacccgg gcaccggcgt cgtgaccatc ttcgacaacg tggacctcga tcgtccctac
     4441 ggccgcggcg tggtgatcca cgagctgacc cacgcgctgg tcgatcagcg cttcgacctc
     4501 gacttggtgg gacgggccat cgtggacggc gactccttcc tggccacctc gtccctggtg
     4561 gagggcgacg cgatgctcgc ggagcatctg tggctcgagc ggaccgcccc cgaggagccg
     4621 tcgccgttcc atggataccg ggaccccgag gacggcgagg cgctcttcgc cggcttcccg
     4681 tacgtccggg gctacgagtt cgttcgttcc ctctacgacg cgggcgggtg ggaggcgctc
     4741 aacgaggcgt atcgccggcc tccggtgacc agcgagcagg tcctccaccc ggaggcctac
     4801 ctggccggtg actcgggggt acccgcccca gaagaggtga tgtcgcccgc gcccgggctc
     4861 ggtgcccagt tcgaggacgg tcgcgtcggc gagctcttcc tgcgcgggct gctcggaccg
     4921 accgcgccgg cgtccgcctc ggagggatgg gacggcggcg cctaccacgc cttccgcgac
     4981 gacgagggcc gggtctcggt ccacgtcgtc ctggcgtggg acgcgccgtc ggacgtcggg
     5041 gagttcgtgg gtgcgctcgg acgctgggcg gccgaggcgt tcggcgggtt caccgacttc
     5101 ggaccgcggg agaccggcca ccgttacggt gccgggtccc actgcatcga catggccgta
     5161 gacgagttcc atgccctggt cacgttcacc gtgatcgggc acccctgcga cgcctgagcg
     5221 gcgctgtcgg tccccccgcc tacactcact ccgtggatcg gcggatcttc gggctggaga
     5281 acgagtacgg cgtgacctgc accttccggg ggcagcgccg gctctccccg gatgaggtgg
     5341 cccggtatct gttccgccgg gtggtgcact ggggccgctc gtcgaacgtc ttcctcgaga
     5401 acggcgctcg cctctacctc gacgtcggct cgcaccccga gtacgccacc ccggaatgcg
     5461 actcggtccg cgacctggtg gcccacgaca aggccgggga gcggatcctg gaggcactgc
     5521 tcggggcggc cgaagtgcgc ctgcacgagg aggggatctc cggggacgtc tacctgttca
     5581 agaacaacac cgactcggcc ggaaactcct acggctgcca cgagaactac ctggtggcgc
     5641 ggcagggaga gttcgcgcgg atggcggacg tgctgatccc gttcttcgtc acccgccaga
     5701 tctggtgcgg cgcggggaag gtgctccacg ggccgcgcgg tgcgcagttc tgcatcgcgc
     5761 agcgggccga gcacatctgg gagggcgtct cgtcggccac cacccgctcc cggccgatca
     5821 tcaacacgcg cgacgagccg cacgccgacg ccgagcgctt ccgtcgcctg cacgtcatcg
     5881 tcggcgactc gaacatgagc gagttcacct cgtggctgaa ggtcggcgtg accgacctcg
     5941 tcctgcggat ggtcgaggag aacgccgtca tgcgcgacct gacgctcgag aacccgatcc
     6001 gcgcgatccg cgagatcagc cacgacatga cctgccgcaa gaaggtccgg ctggcgaccg
     6061 gacgggagct ttcggcgatc gagatccagg cggagtacct cgagcgggcc gcgcgcttcc
     6121 tcgaccgcca cggcgccgac gcgcaccttg gcgaggtgct cgagcagtgg cgcttcgcgc
     6181 tgcaggcgct ggccgaggga gccctcgagc ggctcggacg gcaggtggac tgggtggcga
     6241 agtatctgct gatcgagcgc taccgcgcca cccacaacat ggggctggcc agcc
//
LOCUS       DASPWO010000466         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1167757, whole genome shotgun sequence.
ACCESSION   DASPWO010000466 DASPWO010000000
VERSION     DASPWO010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167757"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VEO00_10625"
     CDS             <1..318
                     /locus_tag="VEO00_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58489.1"
                     /translation="RDPINLFAVALLVSVAVETVAVALQGFGAVESSGSFSSLLGAAG
                     VASYLAIGMVLATALVAVMIRLTLRGERASAVQAATGFFYLAVIFAVAGEFAAKIRFL
                     PPR"
     gene            401..949
                     /locus_tag="VEO00_10630"
     CDS             401..949
                     /locus_tag="VEO00_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58490.1"
                     /translation="MAATGEGDVGRASELMAKAFRSLARPEQDEVLRALFAATLGAVA
                     GGGMRLGYAAGPPGPLPQLPTPLDPQADVLFLRSATAGIRTLPPAGGEMQMVPMRLPE
                     PQYQAFRRWCQEHSFSMAAVMRGLVESFLEKQAVAQVTPPKRTAPRKRATRSSSERAG
                     PKPPAARRRTRSAAKRAPRRSP"
     gene            984..1613
                     /locus_tag="VEO00_10635"
     CDS             984..1613
                     /locus_tag="VEO00_10635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HYU58491.1"
                     /translation="MAAVVIALGAVAVAVVWRLVARGVATVWGAMPVVLAVAAVVALL
                     AEDVHLSRRIAPVVATLAGAGIGLGLYVATRVFVRLVARFPAFAHHTADVYGERHDVS
                     LAVALFLALAVVAPAEEAFWRGFVQARLGDAMSPLAGALAAWGLYVAANLASGKLPIV
                     AAAVVGGAVWGGLAFWTRGVLASVACHVVWAGLMILLPPPEAASREVSA"
     gene            1640..>2475
                     /locus_tag="VEO00_10640"
     CDS             1640..>2475
                     /locus_tag="VEO00_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYU58492.1"
                     /translation="MLRGGVLCHLAVGWGERPHVTPVVFVAHGGSLWFTTSRTSVKAR
                     AWRRDPAAAGLVRQGARAVAFRGTVRTYDLLDPATWGRSLASVPRLTRAATAFTLRNA
                     RFFAGYARDAARVPLAWTPPGRVFAELGLRAIAVLDLEAGAVVERAGAWPSPRRRPTR
                     AYRRLARVALTDRTVPDDVLDAVGISGEGALGFLDPLTVLPARWRRDAGQGCYLATLP
                     AAFLDLATWREGQAGLTVDRRSAWRAREMRGMLLQGTARPSAPVPDGGEQAKAVARAG
                     GRP"
BASE COUNT          230 a          930 c          946 g          369 t
ORIGIN      
        1 cgcgacccga tcaacctgtt cgcggttgcg ctcctcgtct cggtggccgt cgagacggtg
       61 gccgtggcgc tgcaggggtt cggggccgtc gagtcgagcg gcagcttcag ctcgctgctc
      121 ggcgcggcgg gcgttgcctc ctacctggcg atcgggatgg tgctcgccac cgcgcttgtc
      181 gccgtcatga tccgcttgac gctccgcggc gagcgagcct cggccgttca ggccgccacc
      241 gggttcttct acctggccgt gatcttcgcg gtggctgggg agttcgccgc caagatccgg
      301 ttcctgccac cccgctgacc cgggctccgg cgtgccgcag ccagcgatag cgccgtgata
      361 tcgtgcgata tcacgcggga cggcgaggga gaggagtggc atggcggcga ccggtgaagg
      421 cgacgtcggc cgggcgagcg agctgatggc gaaggcgttc cggtcgctcg cgcggccgga
      481 acaggacgag gtgctgcggg cgctcttcgc ggccacgttg ggagccgtgg cgggcggagg
      541 gatgcggctc ggctacgcgg ccgggccgcc gggtccgctg cctcagctcc ccacgccgct
      601 cgacccgcag gcggacgtgc tgttcctgcg ctcggccacg gcgggcatcc gcacgctccc
      661 gccggccggt ggcgagatgc agatggtgcc gatgcgcctg ccggagccgc agtaccaggc
      721 ctttcgccgc tggtgccagg agcactcctt ctccatggcc gccgtcatgc gcggcctggt
      781 cgagtccttc ctcgagaaac aggcggtggc ccaggtgacg ccgccgaagc gcacggcgcc
      841 gaggaagcgc gccacgcggt cctcgtccga gcgcgcgggg ccgaaacccc cggccgcgag
      901 gcggcgcacg cgatccgccg cgaaacgggc gccacggcgc tcgccctgag cccgtcgcgg
      961 gacccccgct aggctggccg ttcatggccg ccgtcgtgat cgcgcttggg gccgtcgccg
     1021 tggccgtcgt ctggcggctc gtggcgcgcg gcgtggccac cgtgtggggc gcgatgcccg
     1081 tcgtgctcgc tgtcgcggcc gtggttgcgc tgctcgccga ggacgtccac ctctcccggc
     1141 ggatcgcgcc cgtcgtggcc acgctcgccg gcgccgggat cggcctgggc ctctacgtcg
     1201 caactcgcgt gttcgttcgc ctggtggcgc gcttccccgc cttcgcccac cacaccgccg
     1261 acgtctacgg ggagcgtcac gacgtctctc tcgcggtggc gctgttcctt gcgctcgctg
     1321 tcgtcgcgcc cgccgaggag gcgttctggc gcgggttcgt ccaggcgcgg ctcggcgacg
     1381 cgatgtcgcc gctcgccggc gcgctggcgg cctgggggct gtacgtcgcg gcgaacctgg
     1441 cgagcgggaa gctgccgatc gtggccgccg cagtggtcgg cggcgccgtc tggggcgggc
     1501 tggccttctg gacccggggc gtgctggcga gcgtggcctg ccacgtcgtc tgggccgggc
     1561 tgatgatcct ccttccgccg cccgaggccg cgagtcggga ggtctcggcc tgagcgccgc
     1621 gccggcatcc gccgtctcgg tgcttcgtgg gggcgtgctg tgccacctcg ccgtcggctg
     1681 gggcgagcgg ccgcacgtca ccccggtcgt cttcgtcgcg cacggcggca gcctctggtt
     1741 cacgacgtcg cgcacgtccg tcaaggcccg cgcctggcgc cgcgacccgg ccgcggccgg
     1801 cctggtgcga cagggtgcgc gtgccgtcgc gttccgcggc accgtccgga cctacgacct
     1861 gctcgatccg gccacctggg ggcgctcgct cgcctcggtg ccgcgcctga cccgagccgc
     1921 caccgcgttc accctccgca acgcccgatt cttcgccggc tacgcgcgcg acgccgcgcg
     1981 cgtcccgctc gcctggacgc ccccggggcg ggtgttcgcc gagctcggcc tgcgggcgat
     2041 cgcagtgctc gatctcgagg ccggggccgt cgtcgagcgc gcgggtgcct ggccgtcgcc
     2101 gcggaggcgc cccacccgcg cgtaccgccg cctggcgcgg gtggccctca ccgaccggac
     2161 ggtgcccgac gacgtgctcg atgccgtcgg catctccggc gagggcgcgc tcgggttcct
     2221 cgatccgctc accgtcctcc cggcgcgttg gcgccgcgac gccgggcagg gctgctacct
     2281 ggcgacgctg ccggcggcct tcctcgacct cgccacctgg cgagaggggc aggccggcct
     2341 gacggtcgac cggcgctcgg cgtggcgcgc ccgggagatg cgcgggatgc tcctgcaggg
     2401 aaccgcgcgg ccctcggcgc cggtcccgga cggcggcgag caggcgaagg cggtggcccg
     2461 ggccggcggg cggcc
//
LOCUS       DASPWO010000467         5318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_746187, whole genome shotgun sequence.
ACCESSION   DASPWO010000467 DASPWO010000000
VERSION     DASPWO010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746187"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VEO00_10645"
     CDS             <1..675
                     /locus_tag="VEO00_10645"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HYU58493.1"
                     /translation="VWVLCPGPETDPAAAAAAESLARAVGATPVRMDAGRHDRLVALV
                     SHVPQVASTALMRLAAARAEEGEPELLLLAAGGFRDLTRLAASNPDLWADILLANAAR
                     AREGVEAYREALAEVAVLLASGDRAALAALFREARAARLALAAKPQARVGVAILALEI
                     PDRPGTLAEVTSTLGERGINIEDLEIIHSAEGAKGALHLTVGAADADAALAALAARGY
                     AAGRLA"
     gene            690..2027
                     /gene="aroA"
                     /locus_tag="VEO00_10650"
     CDS             690..2027
                     /gene="aroA"
                     /locus_tag="VEO00_10650"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HYU58494.1"
                     /translation="MRFSVRPARRPLRGTVIVPGDKSIAHRWLILAATAEGESRLAGV
                     PASLDVWSTATCLSALRPHREPLVEVEAGLPRAEVERGITLDLTVNASGFGDLRQAGA
                     VLDCGNSATCLRLLLGVLAGRPFGATLDGDGSLRARPMERVAHPLRAMGASVETANGT
                     PPVRMRGGRLSGIEHAPTVPSAQVKGAVLLAGMQADGTTTVRERIPTRDHTERALGAL
                     GAPVSAVDGAISLSAFAHGGFAGRVPGDVSSAAFLLAAAAVVPGSSVTIVGVGVNPSR
                     TGFLEALARMGAAVEIETAGVEVGEPVGSVTVGGAPLRGLSMVPAETARCIDEVPVLA
                     AVAAAAAGETRFAGVAELRVKESDRVAAIVGTIRALGGDAEAEDDDLVVGGGGLAGGA
                     VDALGDHRIAMAFAVAGLAARAPVEIGGMEWVDVSFPGFVPALRSLGAEGGPV"
     gene            2024..2686
                     /gene="cmk"
                     /locus_tag="VEO00_10655"
     CDS             2024..2686
                     /gene="cmk"
                     /locus_tag="VEO00_10655"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HYU58495.1"
                     /translation="MTARRPRVAIDGAAGAGKSTLGRALARALGVPFLNTGAMYRSLA
                     ARALSAGADLEDGEALAALARSVEFAMDAKGDPPGLLIDGRPPGDHLSSPAVERSVSL
                     VARHPAVRRVMAAEQRRLAGGGGVVEGRDIGTVVLPDADVKIFLRASERERAARRVAE
                     REGPAGELASALARRDALDAKVNPLVAAPDAVEIDTEGRDQRAVLDEALGVVSAALAR
                     ED"
     gene            2686..3996
                     /gene="der"
                     /locus_tag="VEO00_10660"
     CDS             2686..3996
                     /gene="der"
                     /locus_tag="VEO00_10660"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HYU58496.1"
                     /translation="MPGVPRVAVVGRPNVGKSTLVNRLLGRRAAIAHPEPGVTRDRVE
                     VTVSWRGRSFLVIDTGGYLPRGGGIDALVREQADRAAFEADVILFVVDGQTGAQQEDL
                     EVARHLRRAEPPVIVVANKLDRPDPSPAEVAELHALGLGEPVAVSALHGYGSADLLDR
                     VLAVLPEPAEDEGEEKPDEARFAIVGRPNVGKSSLFNRLVGEERAVVHDTAGTTRDAV
                     DSLVEVNGRTLRFVDTAGFRKRTRLAGVAYYGLVRALQAIDRSDVALLVIDAADGLTG
                     EDKRIAARTGEAGRGLVAAANKWDLVPAGEREERFLDLKVGLAVFPGVPVLRTSALTG
                     RGVERLVPALLESHATWSKRVSTAKVNEVLQAAQGRRPAPRGVGRPLYGAQVGTGPPR
                     FVIFGSGPVDPGYRRYLENRLRTELGFAGVPIRIAFRQRRKRRR"
     gene            4041..4114
                     /locus_tag="VEO00_10665"
     tRNA            4041..4114
                     /locus_tag="VEO00_10665"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4075..4077,aa:Pro,seq:ggg)
     gene            complement(4080..4721)
                     /locus_tag="VEO00_10670"
     CDS             complement(4080..4721)
                     /locus_tag="VEO00_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58497.1"
                     /translation="MARLLIVRTGLASLVALAACSSGGSPSGTARGSSPTTAPSTATV
                     VAARLGAPPSCPGASVPIGTAGPYGEVVGRSPLWGGFYADVDRDAGTFAAPDAPRTAS
                     GWRIKVLWVMRAGQESAVTIRGADTETGAALWFDPDDYPAGPVATLDPAHPGAGSENP
                     GWLNYPSYLFFPGSGCFRLTVSSRDGGLGDDLRVRGLSSATVVGPAGLEPATF"
     gene            complement(5074..>5318)
                     /locus_tag="VEO00_10675"
     CDS             complement(5074..>5318)
                     /locus_tag="VEO00_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011128415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HYU58498.1"
                     /translation="DGARGGRWMTRDGRSGRFDPAPPPGRIADAYGCGDSFAAGLTYG
                     LAARRSLEDALTLAARCGAACLTGRGPYEAQLRSPG"
BASE COUNT          625 a         1920 c         2097 g          676 t
ORIGIN      
        1 gtctgggtgc tctgccccgg gccggagacc gatccggccg cggccgccgc ggccgagtcg
       61 ctggcgcggg cagtcggggc gacgccggtg cggatggacg ccgggcgcca cgaccggctg
      121 gtggccctgg tcagccacgt cccccaagtc gcctcgacgg cgctgatgcg cctggcggcc
      181 gcccgcgcag aggagggcga gccggagctg ctgctgctgg ccgccggcgg gttccgcgac
      241 ctgacccggc tggcggcgag caaccccgac ctgtgggccg acatcctgct ggcgaacgcc
      301 gcccgcgccc gcgagggcgt cgaggcgtac cgcgaggcgc tggccgaggt ggcggtgctg
      361 ctcgcgtccg gcgatcgcgc cgcgctcgcg gccctgttcc gcgaggcgcg cgccgcgcgg
      421 ctggcgctcg cggcgaagcc ccaggcgcgc gtgggggtgg cgatcctggc gctcgagatc
      481 ccggaccgtc cggggacgct ggccgaggtg acctccaccc tcggggaacg ggggatcaac
      541 atcgaggatc ttgagatcat ccactccgcg gagggcgcga agggcgcgct gcacctcacg
      601 gtcggcgccg ccgacgccga cgcggccctc gccgccctcg ccgcgcgcgg ctacgcggcc
      661 ggacggctgg cgtagggggg tccgggggga tgcggttcag cgttcggccg gcgcggcgtc
      721 cactccgcgg gacggtgatc gttccgggcg acaagtcgat cgcgcaccgc tggttgatct
      781 tggcggccac ggccgagggt gagagccgtc tggccggcgt tcccgcatcc ctcgacgtgt
      841 ggtcgacggc cacctgcctg tcggcgctcc ggccgcaccg tgaacccctg gtcgaggtcg
      901 aggccggcct ccctcgggcc gaggtcgagc gtgggatcac gctcgacctg accgtgaacg
      961 cctctggctt cggggacctg cgccaggcag gcgcggtcct cgactgcggg aactcggcga
     1021 cgtgcctgcg cctgctgctc ggcgtcctgg ccggacggcc gttcggcgca accctcgacg
     1081 gggacgggag cctgcgggcg cggccgatgg agcgcgtcgc ccatcccctg cgggcgatgg
     1141 gcgcctcggt cgagaccgcg aacggcaccc cgccggtccg gatgcgcggc ggccgcctgt
     1201 ccggcatcga acacgcgccc acggtcccga gcgcccaggt gaagggcgcg gtcctgctgg
     1261 ccggcatgca ggccgacggg acgacgacgg tccgcgagcg gatcccgaca cgggaccata
     1321 cggagagggc gctcggcgcg ctcggcgccc cggtgtcggc ggtggacggc gcgatctccc
     1381 tctcggcgtt cgcgcacggc gggttcgccg gacgggtccc cggggacgtg tcctcggccg
     1441 ccttcctcct ggcggccgcc gccgtggtgc ccggctcgtc ggtcaccatc gtgggcgtgg
     1501 gggtcaaccc gtctcgcact gggttcctcg aggcgctggc tcggatgggc gccgccgtcg
     1561 agatcgagac ggccggagtg gaggtcgggg aaccggttgg ctcggtgacg gtcggaggcg
     1621 cgccgctccg cggcctgtcg atggtcccgg ccgagacggc gcgctgcatc gacgaggtgc
     1681 cggtgctcgc cgcggtcgcc gccgccgcgg cgggcgagac gcggttcgcc ggggtggcgg
     1741 agctgcgggt gaaggagtcc gaccgggtcg cggcgatcgt cggcacgatc cgcgcgcttg
     1801 gcggcgatgc ggaggccgag gacgacgacc tggtcgtggg cgggggcggc ctggccggcg
     1861 gggcggtcga tgcgctcggc gaccaccgca tcgcgatggc gttcgctgta gccggcctcg
     1921 ccgcgcgcgc gccggtcgag atcgggggga tggagtgggt ggacgtctcg ttccccgggt
     1981 tcgtgccggc gctgcggtcg ctcggcgcgg aaggcgggcc cgtgtgaccg cccggcggcc
     2041 gcgcgtggcg atcgacggcg ccgccggcgc ggggaagagc acgctcggcc gcgcgttggc
     2101 tcgcgcgctc ggggtgccgt tcctgaacac cggcgcgatg tatcgctcgc tggccgcccg
     2161 cgccctgtcc gccggggccg atctggagga cggcgaggcg ctcgccgctc tggcgcgctc
     2221 ggtcgagttc gcgatggacg cgaagggcga cccgcccgga ctgctgatcg acggacgccc
     2281 gcccggggac cacttgtcct cgcccgcggt cgagcgcagc gtgtccctcg tggcgcgcca
     2341 cccggcggtc cggcgggtga tggccgccga gcagcgccgg ctggccgggg ggggcggggt
     2401 ggtcgagggc cgcgacatcg ggaccgtggt gctacccgac gccgacgtga agatcttcct
     2461 gcgcgcgtcg gagcgcgagc gggcggcgcg tcgcgtggcc gagcgggagg gtccggccgg
     2521 ggagctggcg tccgcgctcg cccggcgcga cgcgctcgac gcgaaggtca atccgctggt
     2581 cgccgcaccc gacgccgtcg agatcgacac cgagggccgc gaccagcggg cggtcctcga
     2641 cgaggcgctc ggtgtcgtat ccgccgcact ggcgcgggag gactgatgcc gggcgtgcct
     2701 cgagtggcgg tggtgggacg gccgaacgtc gggaaatcga cgctcgtcaa ccggctgctc
     2761 ggccggcgcg cggcgatcgc tcaccccgag cccggcgtga cgcgggaccg tgtcgaggtc
     2821 accgtgtcct ggcgcgggcg ttcgttcctg gtgatcgaca ccggcggcta cctcccgcgg
     2881 ggtggcggta tcgacgcgct cgtccgggag caggccgacc gcgccgcgtt cgaggcggac
     2941 gtgatcctgt tcgtggtcga cgggcagacc ggagcgcagc aggaggacct cgaggtggcc
     3001 cggcacctgc gccgcgccga gccgccggtg atcgtcgtgg cgaacaagct cgaccggccg
     3061 gacccgtcgc cagccgaggt ggcggagctg cacgcgctgg ggctcggcga gccggtggcg
     3121 gtctcggcgc tgcacgggta cggctcggcg gacctgctcg accgggtcct ggccgtgctg
     3181 cccgagcccg ccgaggacga gggtgaggag aagccggacg aggcgcggtt cgccatcgtc
     3241 ggccggccga acgtcggcaa gtcctcgctc ttcaaccggc tggtcggcga ggagcgcgcg
     3301 gtcgtgcacg acacggcggg gacgacgcgc gacgcggtgg actcgctggt ggaggtgaac
     3361 ggccggacgc tgcgcttcgt cgacaccgcc ggcttccgca agcggacgcg cctggcaggc
     3421 gtggcgtact acggactggt ccgcgcgctg caggcgatcg accgctcgga cgtcgcgctg
     3481 ctggtgatcg acgccgccga cggcctgacc ggcgaggaca agcgcatcgc ggcgcgcacg
     3541 ggcgaggcag ggcgagggct tgtggcggcg gcgaacaagt gggacctggt cccagccggg
     3601 gagcgcgagg agcggttcct cgacctgaag gtgggcctgg cagtgttccc cggcgtgccg
     3661 gtgcttcgga cctccgccct gaccggccgc ggcgtggagc ggctggtgcc cgcgctcctc
     3721 gagtcgcacg ccacgtggtc gaaacgcgtc tcgaccgcga aggtgaacga ggtgctgcag
     3781 gcggcccagg gccggcgccc ggccccgcgc ggcgtcggtc gccccctgta cggcgcgcag
     3841 gtcgggaccg gcccgccgcg cttcgtcatc ttcggctccg gcccggtcga cccgggctac
     3901 cgccgctacc tcgagaatcg cctccggacc gagctcggct tcgcaggcgt cccgatccgc
     3961 atcgccttcc gccagcgccg caagcgccgc cgctgaccgg tcccgggggg gccgcgggcg
     4021 gatcgctaca atggttgcct cgggccgtgg cgcagcttgg tagcgcgtct gactgggggt
     4081 cagaaggtcg cgggttcaag tcccgccggc ccgacaacgg tggcgctgct caggccccga
     4141 acccgaagat catctcccaa ccccccgtcg cgagagctga ccgtcagccg gaagcagccg
     4201 gacccgggga agaagaggta cgaggggtag ttgagccagc ccgggttctc ggacccggcg
     4261 cccgggtggg cgggatcgag ggtggccacg ggtccggccg ggtagtcgtc gggatcgaac
     4321 cacagtgccg ccccggtctc cgtgtcggcg ccacggatcg tcaccgcgct ctcctgaccg
     4381 gcccgcatca cccacagcac cttgatccgc cagcccgatg cggttcgcgg agcgtcaggg
     4441 gccgcgaagg tgcccgcgtc tcggtcgacg tccgcgtaga agccgcccca gagcggcgac
     4501 cgcccgacga cttcgccgta cgggcccgcc gtcccgatcg gaaccgaggc tcccgggcag
     4561 cttgggggtg cacctagtcg cgcagccacg accgtcgcgg tggagggcgc cgtggtcggc
     4621 gaggaacccc gggcggtacc cgacgggctc ccccccgagg agcacgcggc gagcgccacc
     4681 agggacgcca ggccggtccg aacgatcagg agcctcgcca ccgctcgcct cgtctcttct
     4741 acctcccggc gcgctgtccg gttccgagcg gatgtgcccg ggctagtcat cgtggttcgc
     4801 ccggaatgat cacgttgggc catgttcgcc gggaattcca cgcggtagcg tcatggcaac
     4861 cgagcccagg agcccaccat gcctgccacc gacgaacgca tcacccacga ggacgacgcc
     4921 gcccgagccg aggctgtcgc ccgggcgaaa gcgcggcacc cgagctcccc acgcctggaa
     4981 ccgtctgcgg ccctgccatc cgcggccgcg ccggcgcccc gcgagaccgt ccccgccgga
     5041 tctcgctaga tcgcggccgg ccgggccggc gcctcagccg ggggagcgca gctgggcctc
     5101 gtacgggccg cggcccgtga ggcaggcggc gccgcagcgg gcggcgaggg tcaaggcgtc
     5161 ctcgagcgac cgtctcgcgg ccaggccgta ggtcaacccc gcggcgaagc tgtcgccaca
     5221 cccgtaggca tcggcgatcc ggccgggcgg tggagcggga tcgaaccggc cgctgcggcc
     5281 gtcgcgcgtc atccaccggc ctccacgcgc gccgtccg
//
LOCUS       DASPWO010000468         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_578698, whole genome shotgun sequence.
ACCESSION   DASPWO010000468 DASPWO010000000
VERSION     DASPWO010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578698"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VEO00_10680"
     CDS             complement(<1..223)
                     /locus_tag="VEO00_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HYU58499.1"
                     /translation="MSGPFTRGQPAALAAIRPMIEGRSPHAVLLAGPPSVGKTTLALD
                     LAAGLLCLAQVAADRPCRTCRGCRLVSSGN"
     gene            complement(234..2219)
                     /locus_tag="VEO00_10685"
     CDS             complement(234..2219)
                     /locus_tag="VEO00_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYU58500.1"
                     /translation="MGKNVAVFVDVANIFYAAKAAGVDIDYVTLLKSAVSGRDFVRAY
                     AYTGLDPDNENQRNFHSFLARHGYRVVSKDIRKYGDGKVKANLDIELVVDLMKTARNL
                     DVAIIVSGDGDFAPAIRAVQEMGVRCEVISFRGNTSSDLMDVADLFTDITQIARVEKG
                     SSRSGRRVAGDEADLSMTEVPDKQTEGTGRGRGRTGRGRGRVGEAEPVAATARTGRGR
                     GRAVAAEPEPLAAASGSLIALPGEKLSRVRPAQVAAEPDEDELAGAEELEPTGEPPLI
                     AEGEEADDGRRRRRRRGGRGRGRGRGRDEQPEAVTDALPAAEPDLLDDQVEEPAPARR
                     SPRPAAFGSVWDTQMGVPRQPTSRAPLPPEEEFDDEPAIPEYLIAERRGMTRGPAGGP
                     VGGRGGRARSGYSAAVDRERYGRGGGGGINRYPDVSGRTGGPPRSGRDGGRREERYGR
                     PADRDDRVERPDRGRGRGPQVGGGAPRPGEQWSEVPPELEALLRAQLAAKGGPGGGPA
                     GSAAGRPAGRRLGRPRADASAAPQARTALPSAAAVTEPAADATPKRRPGRPRKTAESP
                     TAASESPVVAAPVSTTRRRTARSTTAAASATETSAGGVAEGADDAPPKRRAARPRTAA
                     PASATDAKAATEESSGDTATAPKRRTTRKSTVTTSEA"
     gene            complement(2282..2683)
                     /locus_tag="VEO00_10690"
     CDS             complement(2282..2683)
                     /locus_tag="VEO00_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU58501.1"
                     /translation="MSPRAADGSSPMDAASLLRQLVYLPQASVFVAEARREVVGGAIL
                     ALRPSVRAGGYVGTVDLLVVDPAHDADRVTDALLEEVLRSAHNKACSAVEAVRPDDPA
                     ERTRWERQGFTEAGPRIARSVAAARVAAGRN"
BASE COUNT          324 a         1048 c          999 g          421 t
ORIGIN      
        1 gattgccgct ggacaccagc cggcagcccc ggcaggttct acagggccga tcggccgcta
       61 cctgcgcgag gcagagcaac ccggccgcga ggtccagggc gagcgtcgtc ttgccgaccg
      121 acggcgggcc ggcgagcagg accgcgtgcg gcgaacgccc ctcgatcatc ggccggatcg
      181 ccgccagtgc cgccggctgg ccccgggtga acggtccgct cacgaggaac cggtcaggcc
      241 tctgaggtgg tcaccgtcga cttgcgggtc gttcggcgct tcggtgcggt ggccgtgtcg
      301 cccgacgatt cttcggtcgc cgccttggcg tctgtcgctg aggccggtgc cgcggttcgc
      361 ggccgggcgg cgcgtcgctt gggcggagcg tcgtcggccc cctcggccac cccacccgcc
      421 gacgtctcgg ttgcactggc cgctgccgtc gtgctgcggg cggtccggcg gcgggtcgtc
      481 gacaccggcg ctgccacgac gggcgactcg ctcgccgccg ttgggctctc ggcggtcttg
      541 cgcggccggc ccggccgtcg cttgggcgtc gcgtcggcgg ccggctctgt aacggcggcc
      601 gccgacggca gcgccgttct ggcttgcggg gcggcggaag cgtcggccct gggccgtccc
      661 aggcgtcggc ccgccgggcg accggcggcg ctgccagccg ggccaccgcc tggaccaccc
      721 ttggcggcca actgggcgcg caggagcgcc tcgagctccg gcggcacctc gctccattgc
      781 tctccgggtc gcggcgcgcc gccacccacc tgaggaccgc gaccgcggcc acgatccggt
      841 cgctcgaccc gatcgtctcg gtcggcgggc cggccgtagc gctcctcccg gcggccgcca
      901 tcgcgaccac ttcgcggcgg gccaccggtc cgcccgctca cgtctgggta gcggttgatg
      961 ccgccgccgc caccgcgccc gtatcgttcg cggtcgacgg ccgccgagta gccgctcctg
     1021 gctcggccgc cgcggccgcc cacgggtccc ccggctgggc ccctggtcat gccgcgcctt
     1081 tcggcgatca ggtactccgg gatcgccggc tcgtcgtcga attcttcctc cggtggcagt
     1141 ggggcgcggc tcgtcggctg gcgcgggacg cccatctgcg tgtcccagac ggacccgaac
     1201 gcggccggcc gaggcgaacg tcgcgccggt gccggctcct cgacctggtc gtcgagcagg
     1261 tcgggctcgg cagccggaag agcgtcggtc acggcctcgg gctgctcgtc gcggccgcgg
     1321 ccccggcccc gtccgcgacc gccgcgccgg cgccgccggc gccggccgtc gtcggcctcc
     1381 tcgccctcgg cgatgagcgg cggctcgccg gtcggctcga gctcctcggc ccctgccagc
     1441 tcgtcctcgt cgggctccgc cgcgacctgt gccggccgaa ccctggacag cttctcgccc
     1501 ggcagcgcga tcagcgagcc actggctgcg gccagtggct ccggctctgc cgccacggcc
     1561 cggccacggc cccggccggt ccgggcagtg gcggcaaccg gctcggcctc gcctacccgt
     1621 ccccgtccgc ggcccgttcg gcctcgccct cgaccggtcc cctcggtctg cttgtcggga
     1681 acctccgtca tggacagatc ggcctcgtcg ccggctacgc gacggccgga tctcgacgag
     1741 cccttctcga cgcgcgcgat ctgggtgatg tccgtgaaca ggtcggccac gtccatgaga
     1801 tcggacgagg tgttgccacg gaaggagatg acttcgcagc ggacgcccat ctcctggaca
     1861 gcccggatgg ccggcgcgaa gtcgccgtcg cccgagacga tgatcgccac gtccaggttc
     1921 cgggccgtct tcatgaggtc gactacgagc tcgatgtcga ggttcgcctt caccttgccg
     1981 tcgccgtact tgcggatgtc cttgctgacg acccggtagc cgtggcgggc caggaaggaa
     2041 tggaagttgc gctggttctc gttgtcggga tcgaggcccg tgtacgcata ggcgcgtacg
     2101 aagtcgcggc cggaaacggc cgacttgagg agcgtgacgt aatcgatgtc cacgccggcg
     2161 gccttggccg catagaagat gttcgccacg tccacgaaga cggcgacgtt cttacccacg
     2221 atgactcctt gatgtgtcgt gtcggtggcc ggcgcgctgc gctgctcggc gaccggattg
     2281 ttcagttgcg gccggcagcg acgcgcgccg cggccacgga tcgtgcaatg cgcggaccgg
     2341 cttcggtgaa tccctggcgc tcccagcggg tccgctccgc gggatcgtcg ggccgaaccg
     2401 cctccacggc ggaacaggcc ttgttgtgcg ccgaccggag gacctcctcc agcagcgcgt
     2461 cggtcacccg gtcggcgtcg tgtgccgggt ccaccaccag caggtcgacg gtccccacgt
     2521 agccgccggc ccggacggag ggccggagcg cgaggatcgc gccaccgacg acctcacgcc
     2581 gcgcttccgc gacgaagacg ctcgcctgag gcagatagac cagctggcgc agcagagagg
     2641 ccgcatccat cgggctcgag ccgtccgcgg cgcgcgggct cacctcgcac agttgcgcga
     2701 gacgctcgat atcggtgatg cgggccgttc tgatcaaata ctccacgccg aaggtgcacc
     2761 ctcatgaggt gatggccgcc gaagccattg ag
//
LOCUS       DASPWO010000469         5431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_834397, whole genome shotgun sequence.
ACCESSION   DASPWO010000469 DASPWO010000000
VERSION     DASPWO010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834397"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VEO00_10695"
     CDS             <1..691
                     /locus_tag="VEO00_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYU58502.1"
                     /translation="SLERLAAECSAVPRAGGSVRLRTAVRERFRRAKDDPETPLQPPR
                     VLYELRRALGREDVLVSDVGLHKLWIGRMFPAYEPGTVMISNGLAAMGFALPAAIAAK
                     LVHPDRRVVAVSGDGGFLMNAQELETAVRLGTAVVNVVWEDGRFGSIAWKQRRRFGRT
                     FGTDFGNPDFVALAESFGMPAWRCEDASDFAKRLAEALAAGEPSLIVIPIDYSPDVAI
                     VGELGEETVPT"
     gene            691..2154
                     /locus_tag="VEO00_10700"
     CDS             691..2154
                     /locus_tag="VEO00_10700"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HYU58503.1"
                     /translation="MTAEREQAVVAGVWTQLSIGGTWRDARGERTFPVEDPSTRQVLA
                     EVADAGPEDGLDAVAAAAGAQDGWAAHPPRERGEILRRAFETIVERTEDLALLMTLEM
                     GKPLAESRAEIAYAAEFFRWFSEEAVRVAGHFAVAPDGRGRLLTMRQPVGPCLLITPW
                     NFPMAMATRKIGPAVAAGCTMILKPASQTPLSALALAQILEECGLPPGVLNLLTTSSP
                     GAVTEPILSDGRVRKLSFTGSTVVGRTLIAQSAEQVLRVSMELGGNAPFIVFEDADLD
                     AAVEAALLAKMRNIGEACTAANRFHVARPLAGEFSGRLAERLGGMRLGRGTEDGVQVG
                     PLIDASAVEKVSGLVRDALDRGAKAIVGAEAGPGPGYFYRPTVLADVPPGSRVLREEI
                     FGPVAPVASFGSEEEAIAAANDTEFGLVAYVFTRDLKRALRVVEGLRTGMVGLNQGFV
                     SNPAAPFGGVKQSGFGREGGREGIEEYLDVKYVAVNL"
     gene            2171..4120
                     /locus_tag="VEO00_10705"
     CDS             2171..4120
                     /locus_tag="VEO00_10705"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HYU58504.1"
                     /translation="MSAGTVAAKLIEPHLVSGTMTPGEEIGIRIDQTLAQDATGTMVM
                     LAFAALDIPRVRTELSAQYVDHNLLQVDWRNADDHLFLRSACERFGVWYSKPGNGVSH
                     PVHMQRLGIPGKSLLGADSHTCAGGSLGMLAIGAGGLEVAMAMAGRPFYLKMPRIFGV
                     RLTGALPEWVSAKDVILEMLRRHGVAGGAGAIIEYYGPGLASMSAMDRHVIANMGAEL
                     GATTTVFPSDEEVRRFMAAERREGDWVDLRADAEEESLYDQHDEIDLGALEPLVALPS
                     SPGNVVPVREVAGQEIYQAVVGSSANPGLRDFAIAAAIVEGRQSHDRVSFDVNPTSRE
                     ILEQLAETGALADLVAAGARVHQAGCMGCVGMGQAPASGENSLRTVPRNFPGRSGVKD
                     DRVFLASPETTAASALTGVITDPRDLGIPYPRLELPASSAPNTAMLVPPGQDGSGVEL
                     RVGPNIAPLPELTPLPDALEAPVLLAVGDDVSTDEIMPAGSSILALRSNIPEIAKHAF
                     VYMDDTLYERAMAVRDTTGWIVVGGRNYGQGSSREHAALAPRYLGLRAVIAVSFARIH
                     WQNLVNFGILPLTFADPVDRAAVEQGHVLALPNVRAEIEGGRTLTVEDRTSGRTFLAE
                     HQLSPRQVRVVLIGGLLNAFRSDRA"
     gene            complement(4126..4680)
                     /locus_tag="VEO00_10710"
     CDS             complement(4126..4680)
                     /locus_tag="VEO00_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58505.1"
                     /translation="MPRTIAALALAATLAACSGSGGTTDSSPRTTGAGTVSPNESPPP
                     QNSTARVGETLTLHGTEEGSAIEVTVAEVIDPYTLQSEVLVPEEGTRLIAFDLELTNA
                     GTVAYSDAPGNGAVLLDENDVEWNSVISGEDVALVSVTMAPGDSDSGLIYFQLPEDLG
                     IARLRLTLNSGYGPESGEWLLDGS"
     gene            4849..5226
                     /locus_tag="VEO00_10715"
     CDS             4849..5226
                     /locus_tag="VEO00_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HYU58506.1"
                     /translation="MRLAPAVSWIPGLMAAVYAVMQRSVRLRQATGTLSVTAVGMFAG
                     GGGHGIGPATILPGVLVGGASPRSRVVGGRIEVRDVLDLTITLDHRVVDGAPAARFGA
                     ELRRLLESPELVLRPRAAEVPQA"
     gene            complement(5217..>5431)
                     /locus_tag="VEO00_10720"
     CDS             complement(5217..>5431)
                     /locus_tag="VEO00_10720"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYU58507.1"
                     /translation="PYRFSRNPMYVAWTLIYAGVALAMNTAWPFVILPFVLLWMHLVV
                     VREERELQRRFGAEYRAYVSRTRRYA"
BASE COUNT          719 a         1869 c         2065 g          778 t
ORIGIN      
        1 gagcctggag cggctggccg ccgagtgctc ggcggtgccg cgcgccgggg gctcggtgcg
       61 gctgcgcacg gctgtccggg agcggttccg ccgggcgaag gacgatcccg agaccccgct
      121 gcagccgccg cgcgtgctct acgagctgcg ccgggccctc gggcgggagg acgtcctcgt
      181 ttcggacgtg gggctgcaca agctctggat cggccggatg ttccccgcgt acgagcccgg
      241 caccgtgatg atctcgaacg gcctggccgc gatgggcttc gcgctcccgg cggccatcgc
      301 ggcgaagctc gtgcacccgg accgccgcgt ggtcgcggtg agcggggacg gcggcttcct
      361 gatgaacgcg caggagctcg agacggcggt gcggctcggc acggccgtcg tcaacgtggt
      421 ctgggaggac ggacgattcg gctcgatcgc ctggaagcag cggcggcggt tcgggcggac
      481 gttcgggacc gacttcggca acccggactt cgtcgccctc gccgagtcct tcggcatgcc
      541 ggcctggcgc tgtgaggacg cctcggactt cgcgaagcgc ctggcggaag cgctggcggc
      601 aggcgagccg tcgctgatcg tgatcccgat cgactactcg cccgacgtgg ccatcgtcgg
      661 cgagctcggg gaggagacgg tgccgacgtg atcaccgcgg agcgggagca ggcggtcgtc
      721 gccggcgtgt ggacgcagct ctcgatcggc gggacgtggc gggacgcgcg cggcgagagg
      781 acgttcccgg tcgaggaccc ctcgacccgc caggtcctgg ccgaggtggc cgacgccggc
      841 cccgaggacg gcctggacgc ggtggcggcc gccgccgggg cgcaggacgg ctgggccgcg
      901 cacccgccgc gggagcgcgg cgagatcctg cgccgggcct tcgagacgat cgtcgagcgg
      961 accgaggacc tggcgctgct gatgacgctc gagatgggga agccgctcgc cgagtcgcgc
     1021 gccgagatcg cctacgcggc ggagttcttc cgctggttct ccgaggaggc cgtgcgggtc
     1081 gcgggacact tcgccgttgc gccggacggc cgcgggcggc tgctgaccat gcgccagccg
     1141 gtcgggccct gcctgctgat cacgccctgg aacttcccga tggcgatggc cacgcgcaag
     1201 atcggtccgg ccgtcgcggc cggctgcacg atgatcctga agcccgcgag ccagactccg
     1261 ctctcggccc tggcgcttgc gcagatcctc gaggagtgcg gcctgccgcc cggcgtgctc
     1321 aacctgctga ccacgtcgtc gcccggtgcg gtgaccgagc cgatcctctc cgacggccgc
     1381 gtccgcaagc tgtcgttcac cggctccacc gtggtcggcc ggacgctcat cgcgcaatcg
     1441 gccgagcagg ttctgcgcgt gtcgatggag ctcggcggga acgcgccgtt catcgtcttc
     1501 gaggacgccg acctggacgc cgcggtggag gcggccctgc tggcgaagat gcgcaacatc
     1561 ggcgaggcgt gtaccgccgc gaaccggttc cacgtcgcaa ggccgctggc cggggagttc
     1621 tccgggcggc tggcggagcg gctggggggc atgcgcctgg ggcggggtac cgaggacggg
     1681 gtccaggtcg gtccgctgat cgacgcgtcg gcggtggaga aggtgtccgg cttggtccgc
     1741 gacgcgctcg accgcggggc gaaggccatc gtcggcgcgg aggccggccc gggcccgggg
     1801 tacttctacc gcccgacggt cctcgccgac gtcccccccg gctcccgggt gctgcgcgag
     1861 gagatcttcg gtccggtggc ccccgtcgcg tcgttcgggt ccgaggagga ggcgatcgca
     1921 gcggcgaacg acacggagtt cggcctggtg gcctacgtgt tcacccgcga cctgaaacgg
     1981 gcgctgcggg tggtggaggg cctgcggacc ggcatggtcg ggctgaacca ggggttcgtc
     2041 tccaacccgg ccgcgccgtt cggcggcgtg aagcagtctg ggttcggccg ggagggcggc
     2101 cgcgagggca tcgaggagta cctcgacgtg aagtacgtgg cggtgaacct ctgatggcgg
     2161 ggagcctcgc gtgagcgcgg ggaccgtcgc ggcgaagctg atcgagccgc acctcgtctc
     2221 ggggacgatg acccccggcg aggagatcgg gatccgcatc gaccagaccc tcgcccagga
     2281 cgccaccggc acgatggtca tgctcgcgtt cgccgcgctc gacatcccgc gcgtgcgcac
     2341 cgagctgtcc gcgcagtacg tggaccacaa cctgctccag gtcgactggc gcaacgccga
     2401 cgaccatctc ttcctgcgca gcgcctgcga gcgattcggt gtctggtact cgaagcccgg
     2461 caacggcgtg agccatcccg tccacatgca gcggctcggc atcccgggga agtcgctgct
     2521 cggggcggac agccacacgt gcgccggcgg ctcgcttggg atgctggcga tcggcgccgg
     2581 cggcctcgag gtggcgatgg ccatggccgg tcggccgttc tacctgaaga tgccccggat
     2641 cttcggcgtg cgcctcaccg gggcgctgcc ggagtgggtc agcgcgaaag acgtcatcct
     2701 ggagatgctc cgccgccatg gcgtcgcggg cggcgcgggc gcgatcatcg agtactacgg
     2761 gcccgggctc gcgtccatgt cggccatgga ccgccacgtg atcgccaaca tgggcgccga
     2821 gctgggggcg acgaccaccg tgttcccttc cgacgaggag gttcggcggt tcatggcggc
     2881 cgagaggcgg gaaggcgact gggtcgatct gcgcgccgac gccgaggagg aatcgctcta
     2941 cgaccagcac gacgagatcg acctcggtgc gctcgagccg ctcgtcgcgc tgccctccag
     3001 ccccgggaac gtcgtgccgg tccgcgaggt ggccgggcag gagatctacc aggcggtggt
     3061 cgggtcgtcg gccaaccccg ggctacggga cttcgccatc gccgcggcca tcgtcgaggg
     3121 ccgccagagc cacgaccgag tctccttcga cgtgaacccc acgtcccggg agatcctcga
     3181 gcagctcgcg gagacggggg cgctcgccga cctcgtggcg gccggcgccc gcgtgcatca
     3241 ggcggggtgc atgggctgcg tcgggatggg gcaggcgccg gcctcgggcg agaacagcct
     3301 gcggaccgtg ccgcggaact tccccggccg ctccggcgtg aaggacgacc gggtgttcct
     3361 ggcgagtccg gagacgaccg cggcgtcggc gctcaccggc gtcatcaccg acccccggga
     3421 cctcgggatc ccttaccctc gcctcgagct cccggcgtcg tctgcgccga acacggcgat
     3481 gctcgtgccg cccggccagg acggctccgg cgtcgagctg cgcgtggggc cgaacatcgc
     3541 gccgctcccc gagctgacgc cactgccgga cgccctcgag gcgccggtcc tgctcgcggt
     3601 gggagacgac gtctcgaccg acgagatcat gccggccggc tcgagcatcc tcgcgctccg
     3661 cagcaacatc cccgagatcg cgaagcacgc gttcgtgtac atggacgaca cgctgtacga
     3721 gcgggccatg gcggtgcgcg acacgacggg atggatcgtc gtcggcgggc ggaactacgg
     3781 tcaggggtcg agccgcgagc acgccgcgct cgccccgcgg tacctgggcc tgcgggccgt
     3841 catcgcggtg agcttcgccc ggatccattg gcagaacctc gtgaacttcg ggatcctccc
     3901 gctcacgttc gcggatcccg tcgaccgggc ggccgtcgag cagggccacg tgctggcgct
     3961 gccgaacgtg cgcgccgaga tcgagggcgg ccggaccctg accgtcgagg accggacgag
     4021 cggccggacg ttcctggcag agcaccagct cagtccccgc caggtccggg tcgtgctgat
     4081 cggtggcctg ctgaacgcct ttcgctccga tcgggcctga cggggctacg acccgtccag
     4141 cagccactct ccgctctcgg ggccgtaccc gctgttcagc gtgagccgaa gccgcgcgat
     4201 gccgagatcc tcggggagct ggaagtagat caggccgctg tcggagtcgc cgggggccat
     4261 cgtcacgctg accagggcca cgtcctcgcc cgagatgacg ctgttccact cgacatcgtt
     4321 ctcatcgagg aggacggcgc cgttccccgg tgcgtcgctg taggccacgg tgccggcgtt
     4381 cgtcagctcc aggtcgaagg cgatcaggcg tgttccctct tccgggacga ggacctcgct
     4441 ctgcagcgtg tacgggtcga tcacctcggc gaccgtcacc tcgatcgccg aaccctcctc
     4501 ggtgccgtgc agcgtcaggg tctcgcccac gcgggcggtc gagttctgcg gcggcggcga
     4561 ctcgttcggc gagaccgttc cggccccggt tgtccgcggc gagctatcgg tggtcccgcc
     4621 cgagccggag caggcggcga gggtcgcggc gagggcgagg gcggcgatgg ttcggggcat
     4681 ggggggttcc ttctccttcc tggatggtcc gggcggcggc agaatacgcc gtccgcggag
     4741 gggcgctccg gaggggcgct ccacagggcg agctgcgtgg acgagctgac cgccgaggat
     4801 gcgtggggtc aagcgccggc cgggcgaaag cggaacggtc gcctgataat gcggctggcc
     4861 ccggccgtct cgtggatacc cgggctgatg gccgcggtct acgcggtgat gcagcgatcg
     4921 gtccggctgc ggcaggccac ggggacgctg tcggtcacgg cggtcgggat gttcgccggc
     4981 ggcggagggc acggcatcgg gccggcgacg atcctccccg gcgtcctcgt cggtggggcg
     5041 agcccgcggt cacgggtggt gggtgggcgg atcgaggtcc gcgacgtgct ggacctgacg
     5101 atcacgctcg accaccgggt ggtcgacggc gcccccgcgg cgcgcttcgg cgccgagctg
     5161 cgccgcctcc tcgagagccc cgagctggtg ctccggccgc gggccgccga ggtacctcag
     5221 gcatagcggc gcgtgcggga cacgtaggcg cggtactcgg cgccgaagcg ccgctggagc
     5281 tcacgttcct cccgaaccac cacgaggtgc atccacagga gcacgaacgg caggatcacg
     5341 aacggccacg ccgtgttcat tgcgagggcg accccggcgt agatcagggt ccaggccacg
     5401 tacatcggat tgcggctgaa ccggtagggc c
//
LOCUS       DASPWO010000470         3872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_746471, whole genome shotgun sequence.
ACCESSION   DASPWO010000470 DASPWO010000000
VERSION     DASPWO010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746471"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VEO00_10725"
     CDS             complement(<1..392)
                     /locus_tag="VEO00_10725"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HYU58508.1"
                     /translation="MQRAAGAAFVTVTVAVAVAVAAVTVPSGTASRAASGRPVQQVAA
                     RVEVGAVQAPPVLKAAAAAGVAPTSSGVRRAIAPALKDPTLGPHVGAFVYDASRGRTV
                     YGLNAASTFTPASTMKVLTTTAALATLGP"
     gene            443..931
                     /locus_tag="VEO00_10730"
     CDS             443..931
                     /locus_tag="VEO00_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HYU58509.1"
                     /translation="MEFDVFIEIPKGCRNKYEVDHQTGRLRLDRTLFTATQYPADYGF
                     IENTLGLDGDPLDALVLLPEPTFPGCLIKARAIGMFRMKDEKGGDDKVLCVPAGDPRQ
                     EHLRDIHHLPEFDRLEIQHFFEVYKDLEPGKSVEGATWVGRADAEAEIIASFERLKTE
                     GH"
     gene            complement(935..1531)
                     /locus_tag="VEO00_10735"
     CDS             complement(935..1531)
                     /locus_tag="VEO00_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HYU58510.1"
                     /translation="MIIVTAAIKGGVGKTTTAVYLAALASSNRRTATLIDADPSGSAA
                     DWIENHNDERLDRVEIVEAPTERLLTKALDKIEPGATGIVDPPPSHERLVNKAIERAR
                     VIVIPTRIGGVEMPRVESVLEMIPDGTPYGLVICSARTYTRSYAEAVQAWSEAGVPIW
                     GTVPERVAITAGPTGPLSYDGLEAYKKVWRKVLAAARS"
     gene            complement(1750..3219)
                     /locus_tag="VEO00_10740"
     CDS             complement(1750..3219)
                     /locus_tag="VEO00_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HYU58511.1"
                     /translation="MPTPTGATPESRGVCARKAGRVVLAGALTVSLTGSVALISAPAM
                     ADPKPPVIPSQNQVDAAKKAVGDKAAQVNAVEARLAAGTARLEALGRAGDRAAEAYNG
                     AVYRLKLAKAEAAAAEQRARKAQADLDAQGRQIGRFAAASYQSGGGLARIAPLFTADG
                     TQSLLDRAGTAQSVSGAMQASYLRYSASRVVTNAFKLQAEQALAEVRKATAAAAAAKA
                     RAEAAVKAQQVAVAGAAVERRQLIGQLASLRKVSYQVAAQRQKGLEELARQRAAAEAA
                     RRAAELRRRAAEKAAAEAAARAAEAARKAAQRAERAKAERAREAAERAERAAREAREA
                     RDQKAREEAARRAQEAARDAERQTGSGGSSEGTRSGAERAVSFARAQLGEPYVWGADG
                     PDSWDCSGLTMGAWERGGVDLPHYSAAQYEQIGHISADDLRPGDLIFWADDPDDPATI
                     FHVALYIGDGYMIHAPRPGASVRIDSVYYWVDPDFFGRP"
     gene            3674..>3872
                     /locus_tag="VEO00_10745"
     CDS             3674..>3872
                     /locus_tag="VEO00_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014050226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58512.1"
                     /translation="MTNSVSAATAAATYTITVGTKGSWTHPTDTPAGAYTDKDGTFYF
                     QQSAALYGSTDPRKWDFYTGTN"
BASE COUNT          535 a         1435 c         1316 g          586 t
ORIGIN      
        1 gggccgagcg tggccagcgc ggcggtggtg gtgagcacct tcatcgtcga cgccggggtg
       61 aacgtcgacg cggcgttcag gccgtagacc gtcctgcccc gcgacgcgtc gtacacgaac
      121 gcgcccacgt gcgggcccag cgtcgggtcc ttcaacgccg gggcgatggc ccgtcgtacg
      181 cctgaggacg tcggcgcgac gcctgcggcg gcagccgcct tcagcaccgg cggggcctgc
      241 acggccccga cctcgacgcg ggccgcgacc tgctgcaccg ggcggccgga cgccgcgcgc
      301 gacgccgtcc ccgagggcac ggtcacggcg gcgacggcaa cggcgacggc cactgtgacc
      361 gtgacgaacg ccgcaccggc tgcgcgctgc acgaggcgtc tcctgtcttt cggcgggtcg
      421 gaaccgtgac agtatcgtcc gcatggagtt cgacgtcttc atcgagatcc ccaagggttg
      481 ccgcaacaag tacgaggtcg atcaccagac cgggcggctg cgcctggacc ggaccttgtt
      541 caccgcgacg cagtacccgg ccgactacgg gttcatcgag aacaccctcg gtctggacgg
      601 cgacccgctg gacgcgctcg tcctgctgcc ggagccgacc ttcccgggct gcctgatcaa
      661 ggcccgggcg atcggcatgt tccggatgaa ggacgagaag ggcggggacg acaaggtgct
      721 gtgcgtcccg gccggcgacc cgcgccagga gcacctgcgc gacatccacc acctgccgga
      781 gttcgaccgg ctcgagatcc agcacttctt cgaggtctac aaggacctgg agccgggcaa
      841 gtccgtcgag ggcgcgacct gggtcggccg cgccgacgcc gaggccgaga tcatcgcgtc
      901 gttcgagcgg ttgaagaccg aagggcacta gcgctcagct gcgcgcggcg gcgagcacct
      961 tgcgccagac cttcttgtac gcctccagcc cgtcgtacga cagcggcccc gtcggcccgg
     1021 cggtgatcgc cacccgctcg ggcaccgtgc cccagatcgg cacaccggcc tcggaccagg
     1081 cctgcacggc ctcggcgtag ctgcgggtgt aggtccgggc cgagcagatc accagtccgt
     1141 acggcgtacc gtccgggatc atctcgagca ccgactcgac ccggggcatc tcgacaccgc
     1201 cgatcctggt cgggatcacg atcacccgcg cccgctcgat ggccttgttg accagccgct
     1261 cgtgcgacgg cggcgggtcc acgatcccgg tggcgcccgg ctcgatcttg tccagcgcct
     1321 tggtgagcag ccgctcggtc ggggcctcga cgatctcgac acggtcgagc cgctcgtcgt
     1381 tgtggttctc gatccagtcc gccgcactac ccgacggatc ggcgtcgatc agggtcgcgg
     1441 tacggcggtt cgaggacgcc agcgcggcga ggtacaccgc ggtggtcgtc ttgcccacgc
     1501 cgcccttgat tgctgcggtc acgatgatca tgaccgcgac ggtattccgt tccggccacg
     1561 gaacgataac gaccggcgtg gcgcgcgctc ggccggaccg gtcaggccgc tctgaggccc
     1621 ctcacatgga tatcggcaca tggatatcgg ccggaagcgg gcgacgagtt ccggaacaac
     1681 ccccgcccgg gcgttccaga gccgcgcact tccggccgac atctaggtaa cgaggttgcc
     1741 aagcctaggt cacggccttc cgaagaaatc cgggtcgacc cagtagtaga cgctgtcgat
     1801 ccgcaccgac gcgccggggc gtggggcgtg gatcatgtac ccgtcgccga tgtacagagc
     1861 cacgtggaag atcgtcgccg ggtcgtccgg gtcgtcggcc cagaagatca ggtcgcccgg
     1921 ccgcaggtcg tcggcggaga tgtgcccgat ctgctcgtac tgcgcggccg agtagtgcgg
     1981 gaggtccacc ccgccccgct cccaggcgcc catcgtgagg ccggagcagt cccaggagtc
     2041 gggaccgtcg gcaccccaga cgtagggctc gccgagctgg gcgcgagcga acgagaccgc
     2101 ccgctcggcg ccgctgcggg taccctccga gctgccgccg ctgccggtct ggcgctcggc
     2161 gtcccgagcc gcctcctgcg cgcgccgggc cgcctcctcg cgggccttct ggtcgcgagc
     2221 ctcgcgcgcc tcgcgggcgg cccgttcggc acgctcggcg gcctcccggg cacgctcggc
     2281 cttggccctc tccgcgcgct gcgcggcctt gcgggccgcc tccgcggccc gggcggcagc
     2341 ctcggcggcc gccttctccg ccgcccggcg gcgcagttcc gcggcccggc gggctgcctc
     2401 ggccgcggcc cgctgccgag ccagctcctc caggcccttc tgccgctgcg cggcgacctg
     2461 gtacgagacc ttgcgcagac tcgcgagctg gccgatcagc tgccgccgct cgaccgcggc
     2521 gccggcgacg gctacctgct gcgccttcac cgccgcttcg gcccgcgcct tggccgccgc
     2581 ggccgccgcg gtcgccttcc gcacctcggc cagggcctgc tcggcctgca gcttgaacgc
     2641 gttcgtcacc acgcgggagg cgctgtacct caggtacgac gcctgcatcg cgccggagac
     2701 cgactgcgcg gtaccggccc ggtccagcag cgactgcgtg ccgtccgcgg tgaacagcgg
     2761 ggcgatccgc gccaggccgc cgccggactg gtacgacgcg gctgcgaacc ggccgatctg
     2821 gcggccctgc gcatcgaggt cggcctgcgc cttccgggcc cgctgctcgg cggcggcggc
     2881 ctccgccttg gcgagcttca ggcggtagac ggccccgttg tacgcctcgg cggcgcggtc
     2941 gccggcccgg ccgagcgcct ccagccgggc ggtcccggcc gccaggcggg cctcgaccgc
     3001 attgacctgc gcggctttgt cgccgacggc cttcttcgcg gcgtcgacct ggttctgcga
     3061 cgggatcacg ggcggtttcg ggtccgccat ggcaggagct gagatcaggg cgaccgaccc
     3121 cgtcagcgag acggtgagcg caccggcgag cacaacccgg ccggccttcc tcgcgcacac
     3181 accgcggctt tcaggtgtcg cccccgttgg cgtcggcacg tctgattcct tccgccgggc
     3241 cacgggaccc cttcccaagg gcacgtggca gggacgctct tcttatgtac cttccgtgac
     3301 actagtgctg ttctgtccca aagggaacag ctttcacaca aatcccatcg gctcccaggc
     3361 gtgtcggctt gacaaactac attgttgtaa ttctcgccct cggctgatcg ccgccgatcc
     3421 gtcgttgccg agatgcaggt ggtgtgccgc gagcggggtc acctctgcgt cgacatgctc
     3481 tgaactgttg acttatgtgc agtaatggtc aaacttgtct ccacccgcga ttccgcgtcg
     3541 taccgctaag tcgtccgccc aggggctcaa cgagggggaa gcgccccaag acagaggagt
     3601 ccccgtgtca cgttctcgaa gagcactcgt cgcagcggcc gcctgcttcg gtctcacgct
     3661 gacctcggtc gcgttgacga actcggtgtc ggcggcgacg gccgccgcca cctacaccat
     3721 cacggtcggg accaagggga gctggaccca tcccaccgac accccggccg gtgcctacac
     3781 cgacaaggac gggaccttct acttccagca gtccgccgcg ctctacggca gcaccgatcc
     3841 gcgcaagtgg gacttctaca cgggcacgaa ct
//
LOCUS       DASPWO010000471         6785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_746643, whole genome shotgun sequence.
ACCESSION   DASPWO010000471 DASPWO010000000
VERSION     DASPWO010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746643"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VEO00_10750"
     CDS             complement(<1..459)
                     /locus_tag="VEO00_10750"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYU58513.1"
                     /translation="MGERQVMERTRRLDGMEVPVPGTWVADPAHTTLGFVARHLMVTK
                     VRGRFGEFEGGIHVAERLEDSWAEGVAKAASIDTGVPDRDAHLRSPDFLDVENHPEVR
                     LRLTEFERLEGPRFRVQGELTIRGETRPVTLDATFEGVGTDHTGRARAFFS"
     gene            540..968
                     /locus_tag="VEO00_10755"
     CDS             540..968
                     /locus_tag="VEO00_10755"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYU58514.1"
                     /translation="MDERFEAWVALLEAHAAVMDALDRALQDEGGIPLAWHEVLARLA
                     AQPEGRMRMRDLAREVLLTKSGISRLVDRLQEAGLVTRAECDTDRRVTYATITPDGRR
                     ALRAAGPAFRRTFEERFGRHLSEDEARSLRSTLARVSGRS"
     gene            1112..3592
                     /gene="topA"
                     /locus_tag="VEO00_10760"
     CDS             1112..3592
                     /gene="topA"
                     /locus_tag="VEO00_10760"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HYU58515.1"
                     /translation="MATQLVIVESPTKAKTLERYLGNGYTVRASVGHIRDLPEDRMGV
                     DTERGFEPEYVVPEESRPVVAGLKSALRKADGLVLATDFDREGEAIAFHVAELLGANP
                     STTPRVTFTEITKQAILDAFEEPRTIDMRLVEAQQARRILDRLVGYTVSPLLSKTLRR
                     SLSAGRVQSVAVRLIVEREREIHAFVPVEWWSVDARLTPGDDDLAFTARLFQVGDEKL
                     AASPDKKGLLLSTEAEAEAHVARLREAAYRVAEVREKEVNRNPSAPFTTSTLQQEASR
                     KLGFATRRTMRAAQQLYEGVNLPGEGQVGLITYMRTDSVNIAGSALAEIADVIRRQFG
                     KEYALAEPRHYKKKQRGAQEAHEAIRPTSAVRHPDEIARHLSSDQAKLYRLIWQRTIA
                     SQMAPARFRQLSVDVEARPRDDGPRYLLRATGQVLLFDGFRRVYFEGRDDAFHEDAEA
                     MLPPLTAEQVLRLLDLLPEQHWTEPPPRYSEASLVKALEEYGIGRPSTYASIVSTIVD
                     RGYVRLEDKRFYPEDIGEVVTDLLVESFPDVVDVGFTALMEEELDEIAEGQLGWVQVL
                     DEFFGPFERALEKAADEMAPPEEELDETCPRCPEEGRDPGRLIVKLGRYGKFVACSNY
                     PECTYTREVDGTERPEPEPTGETCPQCVQEGRDPPGSLVKRVGRYGPFVGCDRYPECR
                     YVKKEAAERVGTGVTCPQCGKGELIQRRGRYGLFYSCERFPDCDFALNQKPLAEPCPA
                     CGGVRVEARGGAVRCIACTRAWDGQGEELPEEEALKLVPKPRAKRAPKGGNAASANGS
                     GGTPAARKRTPRATGRRKPAPRRRAPAG"
     gene            3592..5733
                     /gene="tmk"
                     /locus_tag="VEO00_10765"
     CDS             3592..5733
                     /gene="tmk"
                     /locus_tag="VEO00_10765"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HYU58516.1"
                     /translation="MASAAEQIAALRGQRPARLGDLLRHPQFSRLARAMAVSSLGDWV
                     GFVAVAALVTRLGGERAGFAVAGVMAARLLPSVLFGPFAGVLVDRFDRKRLMMTADIS
                     RGLMYAAMPFLRQLGAIYLLSFLIECFALLWVPAKDASLPNLVPRRQIGNANTVGLIT
                     AYGTLPLGGIIFTFLAGLAGTIGIRLDYFANNREFLALWLDGLTFAFSAYMISRLRLR
                     GGPRRREEFRLADAGRDIAGGIRFMREHAMARVMLIGIVMAFTGAGSVMSLGPIFARQ
                     ALNAGDTGWGILVTSLGVGMGLGMAGLGIVSRLVSKETLFGGAMVGAALAAFVLAAMP
                     SIAPAAALTVVVGACAGMTWVTGYTLLQENISDEFRGRTFATLTVLARLGLFLSLAGF
                     PLLAEVVGDHSITVGDARIDLSGTRIALMVGGAVVLLAGIFSRRGLHRTRIARARPLG
                     LHLRMRKAPPSGMFIAFEGVEGSGKGTQIRLLEEYLESTGRPVLVTREPGGTVLGESL
                     RDAVLRKDHESVDARAEALVFAASRAQHVVSVIRPALAEGKIVLCDRYVDSSLAYQGV
                     ARGLGEQDILQLNAWATQGLFPDLVILLHLEPEVGLARKPGEGDRIEDEDDAFHARVS
                     DAYLQLAEEHPERFMVVDADAPPEVVHERVRTVVDRVLTERDADGSLRLGRHDPDRPD
                     SPAPWIPGQDTPPAGTEIPPPPPPPGLEEPG"
     gene            5730..>6785
                     /gene="holB"
                     /locus_tag="VEO00_10770"
     CDS             5730..>6785
                     /gene="holB"
                     /locus_tag="VEO00_10770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HYU58517.1"
                     /translation="MSAVLERVPGQQGAIAFLVRAAADPHHAYLFAGPEGSGKQLAAR
                     AFVAALLCPDGGCGECRACRLALAERHPNVAVVEPEGRDIRVDTIRDEVWTPAYRTAP
                     EPGRKVFVVREADRLNEAAADVLLKVLEEPPPETVLLLLSARPEELPETIRSRCHQVT
                     FHPLSERFVVDALVAEGIPEERALLACRLAGGNLGRARRLAADPEGLAFRDEALQAVD
                     AARAGGAGALAAAEALLAGTATYRKRLKEELAGEMQPVLDPVTGKVDDAYRAVARRIE
                     ERYHRRDRRAEREYLDWSLLALEAWFRDALAAASGTPAPELINLDRAPAVAELAGALE
                     PAEAARAVGAMEDARAAL"
BASE COUNT          905 a         2459 c         2486 g          935 t
ORIGIN      
        1 cgagaagaac gcccgggccc gcccggtgtg atcggtgccg acaccctcga acgtggcgtc
       61 gagcgtcacc ggccgggtct cgccgcggat cgtcaactcc ccctggaccc gaaagcgggg
      121 accctccagg cgctcgaact ccgtcaagcg caggcgcacc tccgggtggt tctcgacgtc
      181 caggaagtcc ggggagcgca ggtgcgcgtc gcgatcgggc accccggtgt cgatcgaggc
      241 cgccttcgcc acgccctcgg cccacgagtc ctccagccgc tcggcgacgt ggatcccgcc
      301 ctcgaactca ccgaaccgcc cgcggacctt cgtcaccatc aggtgccggg ccacgaatcc
      361 cagcgtggtg tgtgccgggt ccgccaccca cgtcccgggc accggcacct ccatcccgtc
      421 cagccgccgc gttcgctcca tgacctgccg ctcgcccatc gccgtcccct ttcgtgccct
      481 ctcgccccat cgtaacggtt gatcccgcaa ccatattccg cgtctgggag aatgggacga
      541 tggacgagcg gttcgaggcg tgggtggcgc tcctggaggc ccacgccgcg gtgatggacg
      601 ccctggatcg ggccctgcag gacgagggcg gcatcccgct cgcctggcac gaggtgctgg
      661 cccgcctggc cgcccagccg gaggggcgga tgcggatgcg cgacctggcc cgtgaggtgc
      721 tgctgacgaa gagcgggatc tcgcgcctgg tcgatcgcct gcaggaggcg gggctggtca
      781 ctcgcgccga atgcgacacc gaccggcgcg tcacgtacgc gacgatcacg cccgacgggc
      841 ggcgggccct gcgggcggcc ggccctgcct tccggcgtac gttcgaggag cggttcgggc
      901 gccacctctc cgaggacgag gcccgatccc ttcgctcgac gctggcgagg gtctccggcc
      961 ggtcatagac ccggtcgaat cggtcgaacc ggacgcgcgt ccgcggccct cgagggcggt
     1021 tcttccggcg ccgtcgggag gtggttctat cctctgcggg gaatatcggg gcgttgacag
     1081 cggagaaacg ctccaggaga aggcaggacc cttggccacc caactcgtca tcgtcgaatc
     1141 gccgaccaag gcgaagacgc tcgagcggta cctggggaac ggctacacgg tccgggcctc
     1201 agtcggccac atccgcgacc tccccgagga tcgcatgggc gtggacaccg aacgggggtt
     1261 cgagcccgag tacgtcgtcc ccgaggagag ccgccccgtc gtcgccgggc tgaagtccgc
     1321 cctcaggaag gcggacggcc tcgtgctggc caccgacttc gaccgcgagg gcgaggcgat
     1381 cgccttccac gtggccgagc ttctcggcgc gaacccctcg accacccccc gcgtcacgtt
     1441 caccgagatc accaagcagg ccatccttga cgccttcgag gagccgcgca cgatcgacat
     1501 gcgcctggtg gaggcgcagc aggcccgccg gatcctcgac cggctggtcg gctacacggt
     1561 ttcgccgctg ctctcgaaga cgctccgccg ctcgctgtcg gccggccgcg tgcagtccgt
     1621 cgccgtgcgg ctgatcgtcg agcgcgagcg tgagatccac gccttcgtcc ccgtcgagtg
     1681 gtggtcggtc gacgcccgcc tgacccccgg cgacgacgac ctcgcgttca cggcccgact
     1741 gttccaggtc ggcgacgaga agctcgccgc ttccccggac aagaagggcc tgctgctctc
     1801 gaccgaggcc gaggccgagg cgcacgtcgc ccggctccgc gaggccgcct accgggtcgc
     1861 cgaggtccgg gagaaggagg tcaaccggaa cccgagcgca ccgttcacca cctccaccct
     1921 gcagcaggag gcctcccgca agctcgggtt cgccacccgg cggaccatgc gcgccgcgca
     1981 gcagctgtac gagggcgtca acctgcccgg cgagggccag gtcgggctga tcacgtatat
     2041 gcgcaccgac tccgtgaaca tcgccgggtc ggcgctcgcc gagatcgccg acgtgatccg
     2101 gcggcagttc gggaaggagt acgcgctcgc cgagccgcgc cactacaaga agaagcagcg
     2161 gggggcgcag gaggcgcacg aggcgatccg gccgacgagc gccgtgcggc accccgacga
     2221 gatcgcccgc cacctctcgt ccgaccaggc gaagctctac cgcctgatct ggcagcgcac
     2281 gatcgcgtcg cagatggccc cggcccggtt ccggcagctg tcggtggacg tggaggccag
     2341 gccacgagac gacgggccgc gctacctgct ccgggccacc gggcaggtcc tgctgttcga
     2401 cggcttccgc cgcgtctact tcgagggccg cgacgacgcc ttccacgagg acgcggaggc
     2461 catgctgccc ccgctcaccg cggagcaggt cctgcggctc ctcgacctgc tgcccgagca
     2521 gcactggacc gagcccccgc cgcgctacag cgaggcctcg ctggtcaagg cgctcgagga
     2581 gtacggcatt gggcgcccct cgacgtacgc ctcgatcgtc tcgacgatcg tcgaccgcgg
     2641 ctacgtccgg ctggaggaca agcgcttcta ccccgaggac atcggcgagg tggtcaccga
     2701 cctgctcgtc gagagcttcc ccgacgtcgt ggacgtcggc ttcaccgccc tgatggagga
     2761 ggagctcgac gagatcgccg aggggcagct cggctgggtg caggtgctcg acgagttctt
     2821 cggcccgttc gagcgggccc tggagaaggc ggccgacgag atggcccccc cggaggagga
     2881 gctcgacgag acgtgcccgc gctgtccgga ggagggccgc gaccccggca ggctgatcgt
     2941 caagctcggg cggtacggca agttcgtggc gtgctcgaac tatccggagt gcacgtacac
     3001 gcgcgaggtg gacggcaccg agcgcccgga gccggagccc accggcgaga cctgcccgca
     3061 gtgcgtccag gagggccgtg acccgcccgg ctcgctcgtc aagcgcgtcg gacggtacgg
     3121 cccgttcgtc ggctgtgacc ggtaccccga gtgccgctac gtcaagaagg aggcggcgga
     3181 gcgggtcggc accggcgtga cctgcccgca gtgcggcaag ggcgagctga tccagcggcg
     3241 cggccgctac gggctcttct actcctgcga gcgcttcccc gactgcgact tcgcgctgaa
     3301 ccagaagccg ctcgccgagc cgtgcccggc ctgcggcggc gtgcgcgtcg aggcccgggg
     3361 cggggcggtg cgctgcatcg cgtgcacgcg ggcctgggac ggccagggcg aggagctgcc
     3421 cgaggaggag gccctgaagc tggtgccgaa gccgcgggca aagcgcgccc cgaagggtgg
     3481 gaacgccgcg tcggcgaacg ggtcgggcgg cacgccggcc gctcgcaagc ggacgcccag
     3541 ggcgacgggc cggcgaaagc cggcgccccg gaggcgggca ccggccggct gatggcatcc
     3601 gccgccgagc agatcgccgc gctccgcggc cagcggcccg cccgcctcgg cgacctcctg
     3661 aggcatccgc agttctcccg cttggcgcgg gcgatggcgg tgtcatcgct cggcgactgg
     3721 gtggggttcg tggcggtcgc cgcgctcgtc acccgcctcg gcggcgagcg cgccgggttc
     3781 gccgtggccg gcgtgatggc cgcgcgcctc ctgccctctg tgctcttcgg gccgttcgcc
     3841 ggggtcctgg tggaccggtt cgaccgcaag cgcctgatga tgaccgccga catctcgcgc
     3901 gggctgatgt acgcggcgat gccgttcctg cgacagctcg gcgcgatcta cctcctgtcg
     3961 ttcctgatcg agtgcttcgc gctgctgtgg gtgccggcga aggacgcgag cctgccgaat
     4021 ctggttccgc ggcgccaaat cggcaacgcg aacaccgtcg gcctgatcac cgcgtacggg
     4081 acgctcccgc tcggcgggat catcttcacg ttcctggccg gcctcgccgg gaccatcggg
     4141 atccggctcg actacttcgc gaacaaccgc gagttcctgg ccctctggct ggacggcctg
     4201 acgttcgcgt tctccgcgta catgatcagc aggctgcgcc tgcgcggcgg gccgcggcgg
     4261 cgcgaggagt tccggctggc cgacgccggc agggacatcg ccggcggcat ccggttcatg
     4321 cgcgagcacg ccatggcccg ggtgatgctg atcgggatcg tcatggcgtt caccggcgcc
     4381 ggctcggtga tgtcgctcgg gccgatcttc gcccggcagg ccctgaacgc cggcgacacc
     4441 ggctggggca tcctggtcac gtcgctcggg gtggggatgg ggctggggat ggccggcctg
     4501 gggatcgtct cgcggctggt gagcaaggag acgctgttcg ggggggccat ggtcggcgcc
     4561 gcgctggcgg cgttcgtgct ggccgcgatg ccgagcatcg cgccggcggc cgccctgacc
     4621 gtggtggtcg gcgcctgcgc ggggatgacg tgggtgaccg gctacacgct cctccaggag
     4681 aacatctcgg acgagttccg cggccggacg ttcgccacgc taacggtgct ggcccgcctg
     4741 ggcctcttcc tgtccctggc cgggttcccg ctgctggccg aagtggtggg cgatcattcg
     4801 atcaccgtgg gagacgcacg gatcgatctg tcggggacgc ggatcgcgct gatggtcggc
     4861 ggggccgtcg tgcttttggc cggcatcttc tcccggcgag ggctgcaccg cacgcggatc
     4921 gcgcgcgccc ggcccctggg gctgcacctg cgcatgcgca aggcgccgcc gtcgggcatg
     4981 ttcatcgcgt tcgagggcgt cgagggatcc gggaagggga cgcagatccg gctgctggag
     5041 gagtacctgg agtccaccgg ccgcccggtg ctcgtcaccc gcgagcccgg cgggaccgtg
     5101 ctcggggaga gcctgcgcga cgccgttctg cgcaaggacc acgaatccgt ggacgcgcgc
     5161 gccgaggcgc tggtgttcgc cgccagccgt gcccagcacg tggtctcggt gatccgcccg
     5221 gcgctcgccg agggcaagat cgtgctctgc gaccgctacg tggactcctc gctcgcctac
     5281 cagggcgtgg cccgcggcct cggggagcag gacatcctgc agctgaacgc ctgggcgacc
     5341 cagggactgt tccccgacct ggtgatcctg ctgcacctcg agcccgaggt cgggctggcc
     5401 cgcaagccgg gcgagggcga tcgcatcgag gacgaggacg acgccttcca cgcgagggtg
     5461 tccgacgcgt accttcagct cgccgaggag cacccggagc ggttcatggt cgtcgacgcc
     5521 gacgcgccgc cggaggtggt gcacgagcgc gtccgaacgg tggtggaccg ggtgctcacc
     5581 gagcgcgacg ccgacgggtc gctgcgcctc ggccgccacg accccgaccg gccggactcg
     5641 ccggcgccct ggatccccgg gcaggacacc ccgcccgccg gcacggagat cccgccgccc
     5701 ccgccacccc cggggctcga ggaaccggga tgagcgcggt cctggagcgt gtcccggggc
     5761 agcaaggggc gatcgccttc ctggtccgcg ccgccgccga cccgcaccac gcctacctgt
     5821 tcgccgggcc cgagggctcg ggcaagcagc tcgccgcgcg cgctttcgtc gcggcgctcc
     5881 tgtgtccgga cggcggatgc ggcgagtgcc gggcctgccg cctggcgctc gccgagcggc
     5941 atccgaacgt cgccgtggtc gagccggagg gccgcgacat ccgggtggac acgatccgag
     6001 acgaggtgtg gacgcccgcc taccgcaccg ctcccgagcc cggacgaaag gtgttcgtcg
     6061 tccgggaagc cgaccgtctg aacgaggcgg ccgccgacgt cctgctgaag gtgctggagg
     6121 agccgcctcc ggagaccgtc ctgctccttc tctccgcgcg gccggaggag ctgccggaga
     6181 cgatccgttc gcgctgccac caggtgacct tccacccgct gtctgagcgc ttcgttgtgg
     6241 acgcgctcgt cgccgagggc atccccgagg aacgggcgct gctggcctgc cggcttgccg
     6301 gggggaacct ggggcgggcc cgccggctgg ccgcggaccc cgaggggctg gcgttccggg
     6361 acgaggccct gcaggccgtc gacgcggcgc gggccggtgg cgccggggcg ctcgccgccg
     6421 ccgaggcgct cctcgccggc accgccacct accgcaagcg gctgaaggag gagctggccg
     6481 gcgagatgca gccggtcctg gacccggtca ccgggaaggt cgacgatgcg taccgggccg
     6541 tggcccggcg catcgaggag cgctaccacc gccgcgaccg ccgcgccgag cgtgagtacc
     6601 tggactggtc gctcctggcg ctggaggcct ggttccgcga cgcgctcgcc gcggcgagcg
     6661 ggacgccggc gccggagctg atcaacctcg atcgcgcccc ggcggtcgcg gagctggccg
     6721 gcgccctcga gcctgccgag gccgcccggg cggtcggggc gatggaggac gcgcgcgccg
     6781 cgctc
//
LOCUS       DASPWO010000472         5565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_490179, whole genome shotgun sequence.
ACCESSION   DASPWO010000472 DASPWO010000000
VERSION     DASPWO010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490179"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2622)
                     /locus_tag="VEO00_10775"
     CDS             complement(<1..2622)
                     /locus_tag="VEO00_10775"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009646131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HYU58518.1"
                     /translation="MAQDMPKAYDATATERRWYEEWLRRGYFEARADTDREPYCIVLP
                     PPNVTGSLHIGHAYQQTNQDVIIRRRRMQGYEALWLPGTDHAGIATQVVVERELAREG
                     TSREELGREAFIQRVWEWKEQYGNRIVHQMQALGNSCDWSRLRFTMDESLSRAVREAF
                     VTLYEDGLIYRGERIINWCPKDGTALSDSEVEHDDVAGELITFAYPLAGGSGRIEVAT
                     TRIETMLGDTGIAVHPDDPRYRDLVGKAVRHPFDGRELPIVADPAVDPSFATGAVKVT
                     PAHDPTDFDIAERTGLPLLNVLDARAHLNENVPERFWGLDRYAARRAVRDALEELGLL
                     VKEERPFVHPVGHCYRCHTEIEPWISGKQWFVAVDRLKGPAMEVAEDGRLAFHPERWT
                     RNYLEWLQGLRDWNISRQLWWGHRIPVWYCANGHEFAAREDPAGCPHCGSADLEQDPD
                     VLDTWFSSQLWPFSTLGWPDDTPDLRTFHPTSVLVTGYEILYLWVARMVMSGMYFTGD
                     VPFRHTMIHGLVRDARGRKMSKSLGNVIDPLDVIESHGADALRFALMRIATGQQDIPL
                     SMDAVEVGRNFANKIWNAARLVLGAGDVPTPAAPGDERSLVERWILSRLEACRDEVDR
                     AIEEYRLADAARALQRFLWAEFCDWGLEMEKARLQDPATAPATAAILAWVLERTLRLL
                     HPIMPFVTEDLWQRLRPEDAAASIVVAPWPEPRPADRDPDAELRFSFAEDLVVALRRF
                     RSDHRIAPSLRLRVRLAGSAEQRSVAEELAAEIGALARLEALELVDAHGEGTGAARLI
                     VQGAEVLIPLEGVLDMGDELRRIERRLAELRGAAESAERKLANLGFTEKAPPDVVEKE
                     RARLVAATDEIATLEVQL"
     gene            complement(2665..2799)
                     /locus_tag="VEO00_10780"
     CDS             complement(2665..2799)
                     /locus_tag="VEO00_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58519.1"
                     /translation="MGPRKHHRTGILGKAWQHAGVWVPLLVIAMLLLALVLGGPSPRY
                     "
     gene            2824..3540
                     /locus_tag="VEO00_10785"
     CDS             2824..3540
                     /locus_tag="VEO00_10785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYU58520.1"
                     /translation="MGGPDPSRPHRRAPAEGEVARMFDAVAPWYDALNALLSGGLDAR
                     WRRAAAGALALPPGSLVLDLGTGSGRLAESLAARYRVVGLDVSRAMLALAVQRATRAG
                     PGRPRHVRGSAFRLPFRDRAFAGAASGFLLRNLDDLPAAFAELARVLVPGGRVALVDI
                     TEPRGPLRRRLFHAYFGRAAPLAGRLSGNSRAYEYLARSLEQLPPPGRVTGLLAAVGL
                     RGRARPLFPGMVTLWTATRA"
     gene            3592..4077
                     /locus_tag="VEO00_10790"
     CDS             3592..4077
                     /locus_tag="VEO00_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYU58521.1"
                     /translation="MGALPEPIEELLNTALVAELTFVDDRGRAVTNPLIPLYDGERVY
                     MTSSVLFSRKLEYLRGNPRVSVAITDPVAVSVERFRRATIQGNARVIDDDLHQGWERV
                     LPLWRAKEPAIDFFLGKRVALPLFFERGIIEITPRRALLWEDGRTELPPSVYELAEVE
                     A"
     gene            4074..5465
                     /locus_tag="VEO00_10795"
     CDS             4074..5465
                     /locus_tag="VEO00_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HYU58522.1"
                     /translation="MSTGGRVDESGILERLEERFAHAILAWVGADGYPISVATGFRVA
                     RERGVVQLDAVATGGEAPPLDREVSVVFSHIRPQPGVGYDERRWVSYWGRLRPVPSGY
                     EFTPESARGWDEQQMPFFEYSERTVPQARRYFERISAERGRRVKPRLAFGWLFLRATR
                     LPFLTATFVPVALGVAVAARDGVFEWRLALLTLLGAAFVHLGVNVANDVFDTLSGADE
                     ANVNPTQFSGGSRVILYGLLSMRQMVAMMAAFYAGAVAIGLYLAVTRGFWPLVGIGAA
                     GVFLSIWYTAPPLRLVHRGLGELCVALGFGPVMVLGAHYVQAQRFSGEALFVSLPVAI
                     LIALVLYVNEIPDRPADAAAGKRTLPVRLSREAVLRVYAIAVTAVFALVAGGAVSGLL
                     PRLTLIALATIPLAIKVYRGARDHYDGPYALMPFMGTGVQLHLFTGLLLLVAYGVTIA
                     AGHVMDAPPALLR"
BASE COUNT          704 a         2003 c         2014 g          844 t
ORIGIN      
        1 aagctgtacc tcgagcgtgg cgatctcgtc cgtcgcggcg accaggcggg cgcgttcctt
       61 ctcgaccacg tctggcggcg ccttctcggt gaacccgagg ttggccagct tgcgctcggc
      121 cgattccgcc gcgccgcgca gctcggccag gcgccgctcg atccggcgaa gctcgtcccc
      181 catgtcgagc acgccctcca gcgggatcag gacctcggcg ccctgcacga tcaacctcgc
      241 cgcaccggtg ccctcgccgt gggcgtcgac cagctccagc gcttcgaggc gggccagcgc
      301 gccgatctcc gcggcaagct cctcggccac cgaccgctgc tcggcggagc cggccagccg
      361 aacccgcagg cggagcgagg gggcgatgcg gtggtcgctt cggaaccgtc gcagggccac
      421 cacgagatcc tcggcgaacg agaagcgcaa ctcggcatcg gggtcgcggt cggccggccg
      481 gggctccggc cacggggcga cgacgatgga cgccgcggca tcctcggggc gcaggcgctg
      541 ccacaggtcc tcggtgacga acggcatgat cgggtggagc aggcgcaggg tccgctccag
      601 gacccaggcc aggatggcgg cggtggccgg agcggtcgcc ggatcctgca gccgggcctt
      661 ctccatctcc aggccccagt cgcagaactc ggcccacagg aaccgctgca gcgcccgggc
      721 cgcgtcggcc agccggtact cctcgatggc ccggtcaacc tcgtcccggc aggcctccag
      781 ccgggagagg atccagcgct cgacgagcga ccgctcgtcc cccggcgcgg cgggggtcgg
      841 gacgtcgccc gcgccgagca ccagccgcgc ggcgttccag atcttgttgg cgaagttgcg
      901 ccccacctcg accgcgtcca tcgacagcgg gatgtcctgc tggccggtgg cgatccgcat
      961 cagcgcgaac cggagcgcgt cggcgccgtg cgactcgatc acgtcgagcg gatcgatgac
     1021 gttgccgagc gacttgctca tcttccgtcc gcgggcatcg cgcaccaggc cgtggatcat
     1081 cgtgtggcgg aacgggacgt cgccggtgaa gtacatgccg gacatcacca tccgcgccac
     1141 ccagaggtag aggatctcgt agccggtgac cagcaccgac gtcgggtgga acgtgcgcag
     1201 gtccggcgtg tcgtcgggcc acccgagcgt ggagaacggc cagagctggg aggagaacca
     1261 ggtgtcgagg acgtcggggt cctgctcgag gtcggcgctc ccgcagtggg ggcagccggc
     1321 cgggtcctcc cgtgcggcga actcgtggcc gttcgcgcag taccagaccg ggatgcgatg
     1381 cccccaccac agctgccgcg agatgttcca gtcgcgcagg ccctgcagcc actcgaggta
     1441 gttccgcgtc cagcgctccg ggtgaaacgc caggcggccg tcctcggcaa cctccatcgc
     1501 cgggcccttc agccggtcga ccgccacgaa ccactgcttg ccggagatcc acggctcgat
     1561 ctcggtgtgg cagcggtagc agtgtccgac gggatgaacg aacggccgct cttccttcac
     1621 cagcagcccg agctcctcga gcgcgtcgcg caccgcgcgg cgcgccgcgt accggtccag
     1681 gccccagaag cgctcgggga cgttctcgtt caggtgggct cgcgcatcca ggacgttcag
     1741 cagcggcagg ccggtgcgct cggcgatgtc gaagtcggtc gggtcgtgcg ccggggtgac
     1801 tttcacggca cccgtcgcga acgaggggtc caccgccggg tccgcgacga tcggcagctc
     1861 gcgcccgtcg aacgggtggc ggacggcttt cccgaccagg tcgcggtagc gcgggtcgtc
     1921 ggggtgcact gcgatcccgg tgtcgccgag catggtctcg atgcgggtgg tggccacctc
     1981 tatccgcccc gacccgccgg ccagtgggta ggcgaacgtg atcagctcgc cggccacgtc
     2041 gtcgtgctcg acctcggaat cggagagcgc ggtgccgtcc ttcgggcacc agttgatgat
     2101 ccgctcgccc cggtagatca ggccgtcctc gtagagcgtg acgaacgcct cgcgcaccgc
     2161 gcgcgacagg ctctcgtcca tcgtgaagcg cagccggctc cagtcgcagg agttcccgag
     2221 cgcctgcatc tggtggacga tgcggttccc gtactgctcc ttccactccc agacccgctg
     2281 gatgaaggcc tcgcggccga gctcctcccg ggaggtgccc tcccgtgcca gctcgcgttc
     2341 caccaccacc tgggtggcga tgccggcatg gtcggtcccc gggagccaca gagcctcgta
     2401 cccctgcatg cgccgccggc ggatgatcac gtcctggttc gtttgctggt aggcatgccc
     2461 gatgtgcagg ctgccggtga cgttcggcgg ggggagcacg atgcagtacg gctcgcggtc
     2521 ggtgtcggcg cgcgcctcga agtacccgcg gcgcagccac tcctcgtacc agcgccgctc
     2581 ggtggcggtg gcgtcgtagg ccttcggcat gtcctgcgcc atcgtgcgac gaaggctagc
     2641 aggcaccctt cgcgacccgc ccgctcagta gcggggcgag gggccgccga gcaccagcgc
     2701 cagcaggagc atggcgatca ccagcagcgg aacccagacg ccggcgtgct gccaggcctt
     2761 cccgaggatc ccggtgcggt ggtgcttgcg cggtcccatc gcggggagtg tacggtccgc
     2821 gtcgtgggtg gacccgatcc gtcacgtccc caccggcggg cgccggccga gggcgaggtg
     2881 gcccggatgt tcgacgccgt cgcaccgtgg tacgacgcgc tgaacgcgct gctctcgggc
     2941 gggctcgacg cgcggtggcg gcgggcggcg gcaggggcac tggcgctccc cccagggtcg
     3001 ctcgtcctcg acctgggcac cggcagcggc cgtctggccg agtcgctggc ggcccgctac
     3061 cgcgtggtgg ggctcgacgt cagccgggcg atgctcgccc tggccgtcca acgcgcgacg
     3121 cgggccggac ccgggcggcc acgccacgtt cgcggcagcg ccttccgcct gcccttcagg
     3181 gatcgggcct tcgccggcgc cgccagcggc ttcctgctcc ggaacctgga cgacctcccg
     3241 gccgcgttcg ccgagctcgc gcgggtgctc gtccccggcg gacgcgtcgc gctggttgac
     3301 atcaccgagc cgcgcggccc gctgcggcgg cgcctgttcc acgcgtactt cgggcgggcc
     3361 gccccgctcg ccgggcggct ctccgggaac tcccgcgcct acgagtacct ggcgcggtcg
     3421 ctggagcaac tgcccccgcc cgggcgggtg accggcctgc tggcggccgt gggcctgcgg
     3481 ggccgggccc gaccactctt cccgggaatg gttacgctct ggacggcgac ccgtgcctga
     3541 gcctcggtgg ctcggtcgcg gtccgcgacc gagacggaga ggggtatcgc gatgggagcg
     3601 ctgccggaac cgatcgagga gcttctgaac acggcgctcg tcgccgagct gacgttcgtc
     3661 gacgaccgcg gccgggcggt caccaacccg ctgatcccgc tgtacgacgg cgagcgcgtc
     3721 tacatgacct cgagcgtgct cttctcgagg aagctcgagt acctgcgcgg caatccccgg
     3781 gtcagcgtgg cgatcaccga tccggtcgcg gtgtcggtcg agcggttccg ccgggccacc
     3841 atccagggca acgcccgcgt gatcgacgac gacctgcacc agggctggga gcgggtgctg
     3901 ccgctctggc gagccaagga gccggcgatc gacttcttcc tcggcaagcg ggtcgcgctg
     3961 cccctgttct tcgagcgcgg gatcatcgag atcacgccgc ggcgcgccct gctgtgggag
     4021 gacgggcgga ccgagctgcc gccgtccgtc tacgagctgg cggaggtgga ggcgtgagca
     4081 ccggcgggcg ggtcgacgaa tcggggatcc tggagcgact cgaggagcgc ttcgcgcatg
     4141 ccatcctggc atgggtgggc gccgacgggt acccgatcag cgtggccacc gggttccgcg
     4201 tcgcccgcga acggggcgtt gtgcagctcg acgcggtggc caccggcggc gaggccccgc
     4261 cgctcgaccg cgaggtgagc gtcgtcttca gccacatccg gccacagccg ggggtcggct
     4321 acgacgagcg gcggtgggtc tcctactggg gtcggctccg cccggtgcca tccgggtacg
     4381 agttcacccc ggaatccgct cggggttggg acgagcaaca gatgccgttc ttcgagtact
     4441 cggaacggac ggtgccccag gcacgccgct acttcgagcg gatctcggcg gaacgcgggc
     4501 gccgggtcaa gccgcgactc gcgttcggct ggctgttcct tcgggccacg cggctgccgt
     4561 tcctgaccgc gacgttcgtg ccggtggcgc tcggcgtggc ggttgccgcc cgcgacggcg
     4621 tgttcgagtg gcgcctggcc ctgctcacgt tgctcggtgc ggcgttcgtg cacctcgggg
     4681 tgaacgtcgc caacgacgtg ttcgacaccc tgtcgggagc ggacgaggcc aacgtcaacc
     4741 ccacgcagtt cagcggcggg tcgcgcgtga tcctgtacgg cctgctgtcg atgaggcaga
     4801 tggtggccat gatggcggcg ttctacgccg gggccgtggc gatcgggctg tacctggccg
     4861 ttacgcgcgg gttctggccc ctcgtcggta tcggggcggc cggtgtgttc ctgtcgatct
     4921 ggtacaccgc tcctccgctg cgactggtgc accgcgggct cggcgaactg tgcgtggcgc
     4981 tcgggttcgg gcccgtgatg gtcctcggcg cgcactacgt ccaggcccag cggttctcgg
     5041 gcgaggcgct gttcgtctcg ttgccggtgg ccatcctgat cgcgctcgtg ctctacgtga
     5101 acgagatccc ggaccgcccc gccgacgccg ccgccggcaa gcgcacgctg ccggtccgcc
     5161 tctcgcgcga ggccgtgctt cgcgtgtacg cgatcgccgt caccgccgtc ttcgcgctgg
     5221 tggcgggtgg cgccgtgagt ggcctgctcc cccggctgac cctgatcgcg ctcgccacga
     5281 tcccgctggc gatcaaggtc taccgcgggg cgcgcgacca ctacgacggc ccgtacgccc
     5341 tgatgccgtt catggggacg ggcgtgcagc tccacctgtt caccggcctt ctcctgctcg
     5401 tcgcctacgg ggtgaccatc gccgccggcc acgtgatgga cgcgcccccc gcgctcctgc
     5461 gctagcgcgg cggcgtgcgg gtgtccgcca ccgcgcgccg ccagcacggc acgaagcccc
     5521 cggggcggcc cgggggcttc gtgatgccct tcagagatcg ctcag
//
LOCUS       DASPWO010000473         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_660492, whole genome shotgun sequence.
ACCESSION   DASPWO010000473 DASPWO010000000
VERSION     DASPWO010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660492"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VEO00_10800"
     CDS             complement(<1..866)
                     /locus_tag="VEO00_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HYU58523.1"
                     /translation="MAEIIPIRSLREAARPDDRCQATTRSGRRCRNRAVDGQGFCRMH
                     SAGPAAARPRPAPAKPERIGPFRAETIEELVDFVRRRLTGEYEIDDFGFDPELTEKVL
                     MRLARPLYERWWRIDFVGLENVPAGGSALLVSNHAGTVPWDSLMMKFGVLDHHPAHRH
                     VRLLAADLAFRMPFIGPLARKSGNTLATEADTFRLLERGELLGVFPEGYKGVGKAFRD
                     RYKLQRFGRGGFIEIALRTKTQIVPVSIVGSEEIYPMIYNARWLARAGGFPYFPITPF
                     FPWLGPLGLIPLP"
     gene            complement(874..1923)
                     /locus_tag="VEO00_10805"
     CDS             complement(874..1923)
                     /locus_tag="VEO00_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYU58524.1"
                     /translation="MAQRVLITGVSRHLGGLLAQRLEQDPDVEYIVGVDLDEPDVDLE
                     RTEYVRADIRNPLVVKVLQTTEVDTVVHLNIIATPTRVGGRSAMKEINVIGTMQLFAA
                     CQKAPALHKVVMKSSTAVYGADARDPALFTEEMGPRSSPRTGYAKDAVEVEQYARDFG
                     RRRPDVDLTVLRFANFMGPRIETPLTRYFSLPAVPTAMGYDPRLQFVHESDAVEVLYR
                     ATREDHPGIYNVSGDGIVLLSQALRLLGKLPIPVIVPLVAPVAGLVRRLGLVDFATDQ
                     LTFLHYGRVADTTRLKEVFGSTPAFSSKEALLDFARGRKIRRLLSAETVSDLEQELYS
                     FLRRKGQENFERTRT"
     gene            complement(2005..>2601)
                     /locus_tag="VEO00_10810"
     CDS             complement(2005..>2601)
                     /locus_tag="VEO00_10810"
                     /inference="COORDINATES: protein motif:HMM:NF042701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HYU58525.1"
                     /translation="RERHAVAVQSERGPRLVGPDNGLLSMAWRALGGAARAVEITSAE
                     VILQPLSATFHGRDVFAPAAAHLALGRPLEFLGPELDPAALVELDLPAPRIGSARVEC
                     VVQAVDRYGNLQLNLGRADLEAAGLADLEVIDVHAADWTIPVRLVKTFADLPLGDLGL
                     LMDSTGAVALFLNQGSAADAFAVESGDEVILGRPGEVE"
BASE COUNT          385 a          905 c          953 g          358 t
ORIGIN      
        1 ggcagcggga tcagcccgag cggcccgagc cacgggaaga acggcgtgat cgggaagtag
       61 gggaacccac cggcccgcgc cagccagcgc gcgttgtaga tcatcgggta gatctcctcg
      121 gacccgacga tcgagaccgg cacgatctgg gtcttggtac gcagagcgat ctcgatgaac
      181 cccccgcggc cgaaccgctg cagcttgtag cggtccctga aggccttgcc gacacctttg
      241 tatccctccg ggaacacccc cagcagctcc ccgcgctcga gcaggcggaa cgtgtctgcc
      301 tcggtggcga gcgtgttgcc cgactttcgc gccagggggc cgatgaacgg catccggaag
      361 gccaggtcgg ccgccagcag ccgaacgtgc cggtgcgcgg ggtggtggtc gaggaccccg
      421 aacttcatca tcagggaatc ccacgggacc gtgccggcgt ggttcgagac gagcagcgca
      481 gaccccccgg ccgggacgtt ctccaggccg acgaagtcga tccgccacca ccgctcgtac
      541 aacgggcggg ccaggcgcat gagcaccttc tcggtcagct cggggtcgaa cccgaagtcg
      601 tcgatctcgt actcgccggt caggcggcgc cggacgaaat cgaccagctc ctcgatcgtc
      661 tcggcgcgga acggcccgat ccgctcgggc ttcgccggcg ccggccgggg ccgggcggcg
      721 gccggaccgg ccgagtgcat ccggcagaac ccctggccgt cgaccgcccg gttccggcag
      781 cgccggccgc tcctcgtggt ggcctggcac cggtcgtcgg ggcgggcggc ctcccgcagg
      841 ctgcggatcg ggatgatctc ggccatgctt atctcacgtc ctggtccgct cgaagttctc
      901 ctggcccttg cggcgcagga aggagtacag ctcctgctcc aggtcgctga ccgtctcggc
      961 gctgagcagc cggcggatct tgcggccgcg cgcgaagtcg agcagcgcct ccttgctgga
     1021 gaacgccggc gtggagccga agacctcctt cagccgggtg gtgtccgcca cgcggccgta
     1081 gtgcaggaac gtcagctggt cggtggcgaa gtccaccagc cccagccggc gaaccaggcc
     1141 ggccaccggc gcgacgagcg gcacgatcac cgggatcggc agcttgccga gcaggcggag
     1201 ggcctgggag agcagcacga tgccgtcgcc ggagacgttg tagatgccgg ggtggtcctc
     1261 gcgggtggcg cggtagagca cctccacggc gtcggattcg tgcacgaact gaaggcgcgg
     1321 gtcgtacccc atggccgtgg ggacggccgg aagcgagaag taccgcgtca gcggcgtctc
     1381 gatccgcggc cccatgaagt tcgcgaagcg caggacggtg aggtccacgt ccgggcgccg
     1441 ccgtccgaag tcgcgcgcgt actgctcgac ctcgacggcg tccttcgcgt agccggtgcg
     1501 cgggctggag cgcggcccca tctcctcggt gaagagcgcc gggtcgcgcg cgtcggcgcc
     1561 gtacacggcg gtggaggact tcatcaccac cttgtgcagg gccggcgcct tctggcaggc
     1621 cgcgaacagc tgcatggtgc cgatgacgtt gatctccttc atcgccgaac ggccgccgac
     1681 gcgggtcggc gtggcgatga tgttcaggtg gaccactgtg tccacctcgg tggtctgcag
     1741 gaccttgacc accagcgggt tgcggatgtc ggcgcggacg tactcggtgc gctcgagatc
     1801 cacgtccggc tcgtcgaggt ccaccccgac gatgtactcg acatcggggt cctgctccag
     1861 acgctgcgcg agcagcccgc cgaggtggcg ggacactccc gtgatcagca cgcgctgcgc
     1921 catacgggga cccaggatag cgggtcccgc accggccccg ccgcggccct tgaggggaac
     1981 acgggggggc ggagcccccg tgcatcactc gacctcgcct ggacggccga ggatgacctc
     2041 gtcgccgctc tcgacggcga aggcgtcggc cgcgctcccc tggttcagga agagcgcgac
     2101 cgcgccggtg gagtccatga gcaatccgag gtcgcccagc gggaggtcgg cgaacgtctt
     2161 caccagccgc accgggatcg tccagtcggc ggcgtggacg tcgatgacct cgaggtcggc
     2221 caggccggcg gcctccaggt ccgcgcggcc caggttcagc tggaggttgc cgtagcggtc
     2281 gacggcctgg accacgcact ccacgcgggc gctgccgatc cgcggcgcgg ggaggtcgag
     2341 ctcgacgagc gcggcgggat cgagctcggg gccgaggaac tcgagcgggc ggccgagggc
     2401 caggtgggcc gcggcggggg cgaacacgtc gcggccgtgg aacgttgccg acagcggctg
     2461 caggatcacc tcggcgctcg tgatctcgac ggcccgggcc gccccgccga gggcccgcca
     2521 ggccatcgac agcagcccgt tgtcagggcc gaccaggcga ggcccgcgct ccgattgcac
     2581 cgccacggcg tgccgctcgc g
//
LOCUS       DASPWO010000474         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_998536, whole genome shotgun sequence.
ACCESSION   DASPWO010000474 DASPWO010000000
VERSION     DASPWO010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998536"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VEO00_10815"
     CDS             <1..869
                     /locus_tag="VEO00_10815"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYU58526.1"
                     /translation="AARTFALGKADLVLAIGVDWDFRKGFATPEPTFIQIDVDAVHMG
                     GNRPADVGIVGDPGRVIEQLVNAGAGFADEPEWTEEVRREERRLHDEARAGMESSAVP
                     IHPQRFAKEIRDSLDPDAIVVGDGGDIVGISASIVHVRYPGHWLDPGPFGCLGAGPSF
                     AMAAKLAKPEKQVLIVYGDGSFGLHAMEYESAIRQKIPFVGVIGNDGAWGQIKVAQEM
                     LYGPGNAPASELDPEAPYHTMVEGLGGYGEKVTSPDQIRPALERAFDSGMPACLNVMV
                     DPALMNRTSYLE"
     gene            866..1657
                     /locus_tag="VEO00_10820"
     CDS             866..1657
                     /locus_tag="VEO00_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58527.1"
                     /translation="MNGPAGVMALVLAETAAGGIAFLFLTPLWREVRPGFFYLTGAVV
                     VALAAGAATTAAGAYEAGAASAGRLAAALAAALGGATVGWLALMVVKLRSAARVLGIA
                     TVPLAVAMLVAFARTTDESLALAIFQLLAGALFTGAVLDGLLLGHWYLTDRKLPRTPI
                     NRMAWLLIGSVVIEAAAVIAGFGSGNASGGSSSNSLNPLLTVGGSASWIAVGMVACTG
                     LIAAFIRLTLRGNRPTAVQSATGFFYLAVITAFTAEIAAKVRLLP"
     gene            1657..>2207
                     /locus_tag="VEO00_10825"
     CDS             1657..>2207
                     /locus_tag="VEO00_10825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HYU58528.1"
                     /translation="MIFALMAVGAAGVVAVWRLVAAGRVTIWAGQAVLFAAAGTAALA
                     TGRVNLSPRVWWTWALLVGSGAGLVLYAATLAFVAGVRRWPPFERHVDQIYDQRRGLT
                     LPAALLLAAAVVAPGEELFWRGLFQGRLAGASGWLVAALVTWAVYVVANAASGSLPIL
                     AAAVVSGGAWGALALWTHGVLASL"
BASE COUNT          281 a          752 c          816 g          358 t
ORIGIN      
        1 ccgcagcccg cacgttcgcc ttggggaagg ccgacctggt cctcgccatc ggcgtggact
       61 gggatttccg gaaggggttc gccacgcccg agccgacctt catccagatc gatgtggatg
      121 ccgtccacat gggggggaac cgccctgcgg acgttgggat cgtcggagat cccggccggg
      181 tcatcgagca gctcgtcaac gcgggagcgg gtttcgccga cgagcccgag tggaccgagg
      241 aagtgcgccg agaggaacgt cgcctgcatg acgaggcccg cgcgggcatg gaatccagcg
      301 ccgtgcccat ccacccccag cgcttcgcga aggagatccg cgactccctc gaccccgacg
      361 cgatcgtggt cggggacggc ggggacatcg tggggatcag cgcctcgatc gtccacgtcc
      421 gatatccggg ccactggctg gaccccggcc ccttcggatg cctcggggcc ggcccgtcgt
      481 tcgccatggc tgccaagctg gccaagccgg agaagcaggt cctgatcgtc tacggcgacg
      541 ggtcgttcgg gctgcacgcc atggagtacg agagcgccat ccgccagaag atccccttcg
      601 tcggggtcat cggcaacgac ggcgcgtggg ggcagatcaa ggtggcccag gagatgctgt
      661 acgggccggg gaacgccccc gcgtcggagc tcgacccgga agctccgtac cacacgatgg
      721 tcgaggggct tggtggctac ggcgagaagg tgacctcgcc ggaccagatc cgcccggccc
      781 tggagcgggc tttcgattcg ggcatgccgg cctgcctcaa cgtgatggtc gatcccgccc
      841 tgatgaacag aacgtcctac ctcgagtgaa cggcccggcg ggcgtgatgg ccctcgtgct
      901 ggccgaaacg gccgccggcg ggatcgcctt cctcttcctg acccccctgt ggcgcgaggt
      961 ccggcccggg ttcttctacc tgaccggagc cgtcgtcgtc gcgctggccg cgggggcggc
     1021 gacgaccgcg gccggcgcct atgaggccgg ggcggcctcg gcagggaggc tggctgcggc
     1081 cctggccgcg gcgctgggcg gggccacggt cggctggctc gcgctcatgg tcgtcaagct
     1141 cagatcggcg gcgcgggtgc tcgggatcgc cacggtccct ctggccgtgg ccatgctggt
     1201 cgccttcgcc cgaaccacgg acgagtccct ggccctggcc atcttccagc tcctggccgg
     1261 cgcgctgttc accggtgcgg tcctggacgg cctgctgctc ggacactggt acctcaccga
     1321 tcgcaagctc ccgcgcacgc cgatcaaccg gatggcatgg ctgctgatcg ggtccgtggt
     1381 gatcgaagcc gccgccgtca tcgccggctt cggctcaggg aacgcatcgg gaggttcgag
     1441 ctcgaactcg ctcaacccgt tgctgacggt gggagggagc gcctcttgga tcgccgtcgg
     1501 gatggtggcc tgcacgggcc tcatcgcggc tttcatccgg ctcaccctgc gaggcaaccg
     1561 cccgacagcc gtccaatcgg ccaccgggtt cttctatctc gccgtcatca ccgcgttcac
     1621 cgccgagatc gcggcgaagg tcaggctgct cccgtgatga tcttcgccct catggccgtg
     1681 ggggcggccg gcgtggtcgc ggtgtggagg ctggtggctg cgggccgcgt cacgatctgg
     1741 gcggggcagg cggtcttgtt cgccgcggcg gggacggcgg cgctggcgac gggccgagtg
     1801 aacctctcgc ctcgcgtgtg gtggacctgg gcgctcctgg tcggctcggg agccggcctc
     1861 gtgctctacg cggcgacgct cgcgttcgtg gcgggggtgc ggcggtggcc gccgttcgag
     1921 cgccacgtcg accagatcta cgaccagcgg aggggcctga cactccccgc ggcgctcctc
     1981 ctggcggcgg ccgtggtcgc ccccggcgag gaactgttct ggcgcgggct gttccaggga
     2041 cggctggcag gagcgtccgg atggttggtg gctgcgctcg tcacgtgggc ggtctacgta
     2101 gtggcgaacg ccgccagtgg cagcctcccc atcctggccg cggcggtcgt gtcgggtggg
     2161 gcgtggggcg ccctggcact ctggacacac ggcgtgctgg caagcct
//
LOCUS       DASPWO010000475         4602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_237527, whole genome shotgun sequence.
ACCESSION   DASPWO010000475 DASPWO010000000
VERSION     DASPWO010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237527"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /gene="hemQ"
                     /locus_tag="VEO00_10830"
     CDS             complement(<1..638)
                     /gene="hemQ"
                     /locus_tag="VEO00_10830"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013019152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HYU58529.1"
                     /translation="MSEPVTYTYFPVFRARPGLRDLGTEGIEAAREVERLEKEWADRV
                     DVRGHYSTVGFRPDADLMLWWVAHSADDLQGLLAAVQRTPLGRHLDLTHAFMGVHRPP
                     EVAKDHVPAFLRGEAPKRYLCVYPFDRSYEWYLLPREERADLLREHGEMGREFPDVLA
                     NTTSNFGIGDWEWILAFEADRLERIVDCIRRLREAPARRHTRTERPFVTGIR"
     gene            complement(635..1570)
                     /gene="hemH"
                     /locus_tag="VEO00_10835"
     CDS             complement(635..1570)
                     /gene="hemH"
                     /locus_tag="VEO00_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HYU58530.1"
                     /translation="MTRTLGLLVMSYGTARDLDDVEAYYTHIRGGRAPAPELVDELRE
                     RYRAIGGRSPLLQITDAQIRALEAALNAGPPPGTAFRAWGGMKHQSPYIEDAVAAMAA
                     DGVREGIGLVLAPHYAGMSVGEYQRRAREAGEAHGVRFAFVDSYHDHPGFVAFVAERV
                     REALDRLPPGVRDGAPVVFSAHSLPERIVAGGDPYPKQLQETADAVAARLGLRSYLTA
                     WQSAGRTPEPWLGPDLVEVLRDLAGQGCTAVVSCPVGFVADHLEVLFDVDVEAQGAAR
                     QAGLILVRTRSLNDDPAFIAVLADVVREQLARGDG"
     gene            complement(1567..2625)
                     /gene="hemE"
                     /locus_tag="VEO00_10840"
     CDS             complement(1567..2625)
                     /gene="hemE"
                     /locus_tag="VEO00_10840"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018836902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HYU58531.1"
                     /translation="MSDPTSDRFLRACRREPVDRTPVWYMRQAGRYLPEYRRIRESHD
                     VLEICRTPDLAVAVSLQPVRRLGVDAAILFSDIMVPVQAMGIDLRIDPDVGPVIPQPV
                     RAAADLRRLRPLDPEGDVPFVLEAVRALAAEAGVPVIGFAGAPFTLASYLVEGGPSRT
                     HPRTKALMLGEPELWDALMSRLGESVLAYLRAQVAAGARAVQLFDSWAGALSLDDYRE
                     RVLPASARILAGLADLGVPRIHFGVNAGHLLEALRDAGAEVVGVDWRTPLDEARARLG
                     EATAVQGNLDPADLLAPWPVLEARALDLLARAGEGTGHVFNLGHGVLPQTNPDALARL
                     TALVHERTVRPAVRGAVR"
     gene            2697..3497
                     /gene="fabI"
                     /locus_tag="VEO00_10845"
     CDS             2697..3497
                     /gene="fabI"
                     /locus_tag="VEO00_10845"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HYU58532.1"
                     /translation="MLLEGKRLLVTGVLTPDSIAYSVAQVAQEEGAEIVLTSFGRAMA
                     VTERTARRLPAPADVLELDANDERQIEAVAADLERRWGAVDGFLHAIAFAPQDALGGN
                     FLRTPWESAATAFRTSAFSLKAIAEGMLPLMREAGGAIVALDFDASVAWPIYDWMGVS
                     KAALESVARYLARDLGGHGIRVNLVSAGPLRTMAAKSIPGFTGLARAWEGRSPLSWDV
                     SDPVPVARTVCFLLSDWARAITAEIVHVDGGYHAMGADLRATDDQPGS"
     gene            3576..4088
                     /locus_tag="VEO00_10850"
     CDS             3576..4088
                     /locus_tag="VEO00_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58533.1"
                     /translation="MRKRIVIALVAALAIGLVGVGMGVGSADVSTPTITIRGEFHFEP
                     NQYIESTQRFVPGIIFVKSGGHLTVRNRDGVREPHTFSIVRQGDVPKTFGEAFGKCFV
                     GACGKILARHGAEGPKTDNGTPGLDGFGDSIILLPGRSVEYTVTAPPGTALYFLCAFH
                     PWMQGRINIG"
     gene            complement(4282..>4602)
                     /locus_tag="VEO00_10855"
     CDS             complement(4282..>4602)
                     /locus_tag="VEO00_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58534.1"
                     /translation="WMRFNRSWISVPTEASPDQRRSRAAVPRPARPTMGGEVVRRVDG
                     SGSVSFAGSGYRVGNRYRGQTVGVRLVGDTVQITLGDQLLRTHRARHDKTKEFGALGT
                     RRAR"
BASE COUNT          627 a         1686 c         1678 g          611 t
ORIGIN      
        1 ttccggatcc cggtgacgaa gggcctctcg gtccgcgtgt gccggcgggc gggcgcctcg
       61 cgcagccggc ggatgcagtc gacgatccgc tcgagccggt cggcctcgaa ggccaggatc
      121 cactcccagt cgccgatccc gaagttgctc gtggtgttcg ccagcacgtc cgggaactcc
      181 cgccccatct cgccgtgctc gcggagcagg tcggcgcgct cctcgcgcgg gaggaggtac
      241 cactcgtagg accggtcgaa ggggtacacg cagaggtagc gcttcggggc ctcgccgcgc
      301 aggaacgcgg ggacgtggtc cttcgccacc tccggtgggc ggtggacgcc catgaatgcg
      361 tgggtcaggt cgaggtggcg gccgagcggc gtgcgctgca ccgcggcgag caggccctgg
      421 aggtcgtccg ctgagtgcgc cacccaccag agcatcaggt ccgcgtccgg gcggaagccc
      481 acggtcgagt agtgcccgcg cacgtcgacc cggtcggccc attccttctc caggcgctcg
      541 acctcccgcg ctgcctcgat gccctcggtc cccaggtcgc gcagccccgg gcgggcccgg
      601 aacaccggaa agtacgtgta ggtgaccggc tcgctcatcc gtcccccctc gccagctgct
      661 cccggaccac gtcggcgagc accgcgatga acgccgggtc gtcgttcagg gagcgggtcc
      721 gcaccaggat cagcccggcc tgccgggccg cgccctgcgc ctcgacgtcc acatcgaaca
      781 gcacctcgag gtggtctgcc acgaacccca ccgggcacga tacgacggcc gtgcatccct
      841 ggccggccag gtcccgcagc acctcgacca ggtcgggacc gagccacggc tccggcgtcc
      901 tgccggcgct ctgccaggcc gtcaggtagc tccgcaggcc gagacgggcc gcaacggcgt
      961 cggcggtctc ctggagctgc ttcgggtacg gatcgccccc ggccacgatg cgctcgggca
     1021 ggctgtgggc cgagaagacg accggcgcac cgtcgcggac gccgggcggg agccgatcga
     1081 gcgcctcccg cacccgctcc gcgacgaagg ccacgaaccc cggatggtcg tggtacgagt
     1141 cgacgaaggc gaaccgcacg ccgtgcgcct cccccgcctc tcgcgcccgg cgctggtact
     1201 cgccgacgct catccccgcg tagtgcggcg cgagcaccag cccgatcccc tcgcgcacgc
     1261 cgtcggccgc catggccgcg acggcgtcct cgatgtacgg ggactggtgc ttcatgccgc
     1321 cccaggcgcg gaacgcggtg cccggtggcg ggccggcgtt cagcgcggcc tcgagcgccc
     1381 ggatctgcgc gtcggtgatc tgcaggaggg gggaacggcc cccgatcgcc cggtaccgtt
     1441 cgcggagctc atccacgagc tccggggccg gtgcgcggcc gccgcggatg tgcgtgtagt
     1501 aggcctcgac gtcgtcgagg tcccgggccg tgccgtagga catgaccagc aggccgagcg
     1561 tgcgggtcac cggaccgccc ctctgacggc cgggcggacg gtgcgctcgt gcaccaacgc
     1621 cgtcagccgg gccagcgcgt cgggattcgt ctgcgggagg acaccgtggc cgaggttgaa
     1681 cacgtgcccg gtcccctcgc ccgcgcgcgc gaggaggtcg agcgcccgcg cctcgaggac
     1741 cggccacggc gcgagcaggt cggccgggtc gaggttcccc tgcaccgccg tcgcttcgcc
     1801 cagccgcgcc cgggcctcgt cgagcggggt gcgccagtcg acgccgacca cctcggcccc
     1861 cgcgtcgcgc agggcctcca gcaggtgccc ggcgttcacc ccgaagtgga tccgcggcac
     1921 gccgaggtcg gccagcccgg ccaggatgcg cgcgcttgcc ggcagcaccc gctcgcggta
     1981 gtcgtccagc gagagcgccc cggcccagga gtcgaagagc tgcacggcgc gcgcgccggc
     2041 cgccacctgc gcgcggaggt aggcgagcac cgactccccc agccgggaca tcaacgcgtc
     2101 ccacagctcg ggttcgccga gcatcagcgc cttcgtccgg ggatgcgtcc gcgacggccc
     2161 accctcgacc aggtagctcg ccagcgtgaa cggggcgccg gcgaacccga tcacgggcac
     2221 cccggcctcg gcggcaagcg cccgcaccgc ctcgagcacg aacggcacgt cgccctccgg
     2281 gtcgaggggc cgcaggcggc gcaggtcggc agcggcgcga acgggctgcg ggatcaccgg
     2341 gccgacgtcg gggtcgatgc gcaggtcgat ccccatggcc tgcaccggga ccatgatgtc
     2401 ggagaacagg atcgccgcgt ccacgccgag ccgccggacg ggctgcaggg acaccgccac
     2461 ggccaggtcc ggggtgcggc agatctcgag cacgtcgtgc gactcgcgga tcctgcggta
     2521 ctcgggcagg tagcggcccg cctggcgcat gtaccagacc ggggtgcggt ccacgggctc
     2581 gcggcggcac gcgcgcagga aacggtcgct cgtcggatcg gacattggga ccgtcagatc
     2641 gtagccgccc ggccggtcgc tcggccgggg cgggggcgga tcggtagcat gggccgatgc
     2701 tgctggaggg gaagcggcta ctggtcaccg gcgtgctgac gcccgacagt atcgcctact
     2761 ccgtggcgca ggtggcccag gaggaaggcg ccgagatcgt gctcacgtcg ttcggccggg
     2821 cgatggccgt caccgagcgc acggcgcgcc gcctgcccgc gcccgccgac gtcctggagc
     2881 tcgacgcgaa cgacgagcgg cagatcgaag cggtggccgc cgatctcgag cggcgctggg
     2941 gagcggtgga cgggttcctg cacgccatcg ccttcgcccc gcaggacgcc ctcgggggga
     3001 acttcctgcg cacgccctgg gagagcgcgg ccaccgcgtt ccgcaccagc gccttctcgc
     3061 tgaaggcgat cgccgagggg atgctccccc tgatgcgcga ggccggcggg gcgatcgtcg
     3121 ccctggactt cgacgcctcc gtcgcctggc cgatctacga ctggatgggc gtcagcaagg
     3181 cggctctgga gtcggtggca cggtacctgg cgcgcgacct cggaggccac gggatccgtg
     3241 tgaacctggt ctcggccggc ccgctgcgga ccatggccgc gaagagcatc ccggggttca
     3301 cgggcctggc gagggcgtgg gagggccgct cgccgctctc ctgggacgtc tcggaccccg
     3361 tcccggtggc gcgcacggtc tgcttcctgc tgtcggactg ggcccgggcg atcaccgccg
     3421 agatcgtgca cgtggacggc ggctaccacg cgatgggcgc ggacctccgg gccaccgacg
     3481 accagccggg atcctgagcg ggaatccggc cggacccctt cccctcggcc gcggctcgtg
     3541 cgatagtccg gccggcggag cgaaaggagt gggggatgcg gaaacggatc gtcatcgcgc
     3601 tggtcgccgc gctcgcgatc ggcctggtgg gcgtggggat gggggtcggg tcggccgacg
     3661 tgagcacgcc gacgatcacc atccgcgggg agttccactt cgagccgaac cagtacatcg
     3721 agagcaccca gcggttcgtg ccggggatca tcttcgtcaa gtcgggcggg cacctcacgg
     3781 tgcgcaaccg cgacggcgtc cgcgagccac acacgttctc gatcgtccgc cagggcgacg
     3841 tgccgaagac cttcggcgag gcgtttggga agtgcttcgt cggagcgtgc gggaagatcc
     3901 tggcgcgcca cggcgccgaa ggaccgaaga cagacaacgg cactcccggg ctggatgggt
     3961 tcggcgactc gatcatcctg ctcccgggcc gcagcgtgga gtacacggtg accgccccgc
     4021 cggggaccgc cctgtacttc ctctgcgcgt tccacccctg gatgcagggc cgcatcaaca
     4081 tcggctgatc gtccgggtcg gcaccgaagg gccgggttcg cccggccctt cggcatctgc
     4141 ggggcaaggc gagcgcgagc ccgaagccgt cgccggcgtc ggcgacctcc tccaccccgg
     4201 acgagtcctg tgtgaaccgc gcgttccggg ccgccgtgag cccgcgcgcc cggccgtaca
     4261 gtgacgtgga cggccccggc ctcatcgagc ccggcgagtg cctagagctc cgaactcctt
     4321 ggtcttgtcg tggcgagcac ggtgggtccg tagcagctgg tcgccgagtg tgatctgcac
     4381 ggtatcgccg acgagacgaa cgccgacggt ctgtccgcgg tagcggttgc ccacccggta
     4441 gccgctgccg gcgaagctga ccgagccgct cccatcgacc ctccgcacga cctcccctcc
     4501 catggtcggc ctggccggcc ttgggaccgc cgctcggctc cgcctctggt cgggagaggc
     4561 ttcggttggc acggatatcc aggacctgtt gaacctcatc ca
//
LOCUS       DASPWO010000476         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_237528, whole genome shotgun sequence.
ACCESSION   DASPWO010000476 DASPWO010000000
VERSION     DASPWO010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237528"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VEO00_10860"
     CDS             complement(<1..420)
                     /locus_tag="VEO00_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58535.1"
                     /translation="MSGRSEEGDRGDLVRLDPATGEIEARIAMDALPSWVVGGGGIAA
                     ASGSIWVMGTNSQGAILQRVDPRANRLADVIPVLPEARDADVWADASGVWALVFEPHD
                     DPHYAVVRVDPLTYRVGEPIEIEATWAHWVFRAGGSVW"
     gene            complement(731..1198)
                     /locus_tag="VEO00_10865"
     CDS             complement(731..1198)
                     /locus_tag="VEO00_10865"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYU58536.1"
                     /translation="MTSRPDLVLEELYREHGGRLWRALVAYAGDPDVASDAMAEAFAQ
                     ALGRGAALREPMRWVWRVSFRIAAGELKDRRRRASGLSPPVTTGDEPADELVGLLSRL
                     SPKQRAAIILHYYVGYSIGEVARVIGSSAATVRVHLSQGRKRLRRILEEEHGG"
     gene            complement(1314..>2606)
                     /locus_tag="VEO00_10870"
     CDS             complement(1314..>2606)
                     /locus_tag="VEO00_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYU58537.1"
                     /translation="LGMVGRRVDSLRIFGRRRTEDSPRLWRRWAHAVMARPWVALIGT
                     VAVVGLLAFPARNLVLGSSGPSILPADSTARQAADITAGAFGAGAVSPIQMVYADPRG
                     VTGDGLEGLLATVGAIAEDPDVVRVDFVLTPLLALNPGATADEARQFLASPLSADVRA
                     QLVAADGDRTLVAIVTTHGPQTAESDATVERLRALVPPVLPEGASAALGGSPGLNLDL
                     NDEVQRQIIPVVALVMILSFVLLMLFFRSLLLPLKAILMNTASVVAAYGVLVFVFQEG
                     HWEGLLGFRSNGHIESFLPLFLFSTLFGLSMDYEVFLLARVREEYLRTGDNTEAVGWG
                     LEHTARIITSAAAIMVTVFGAFAFARLLPIKTMGFGLAVAVLLDATLIRVIMVPATMR
                     LMGRWNWWLPKWLDRILPKVSVEESAPSPDEEKVAAPA"
BASE COUNT          424 a          976 c          864 g          342 t
ORIGIN      
        1 ccacaccgac cccccggccc gaaagaccca gtgggcccag gtggcttcga tctcgatggg
       61 ctcgcccacc cggtaggtga gcggatcgac ccgcacgacc gcgtagtgag ggtcatcgtg
      121 aggttcgaag accagggccc aaaccccgct ggcatcggcc cacacatcgg cgtcgcgcgc
      181 ttccggcagg acggggatca cgtccgcgag ccggttcgcc cgggggtcga ctcgctgcag
      241 gatcgctccc tgcgagttgg tgcccatcac ccagatgctc ccggacgcgg ccgcgatccc
      301 acctcctccg accacccagg aagggagagc gtccatggcg atcctggcct cgatctcccc
      361 ggtcgccgga tccagacgga ccaggtcgcc ccgatccccc tcttcgctcc gaccgctcac
      421 ccagacgctt ccctccgcga tcgagatgga gttgggaagc gttgagatgt cgaccctgcc
      481 ggttacccgc ccctggtccc ggccgggagt cgcggccgac gaagcagcgg aaccgggctt
      541 ctccgagccg gcggtgaagg ctcggacgag gaaggcgata ccggtcgccg cgacggcgag
      601 cgcgacgagg gtggcgaccc aacgtctggg tctgccaggc agccgcttcg ttccaggccg
      661 tcgacgctcg gcatcctgcc atacgtccgg cggctcgatc cggtccaatg cacggaaacg
      721 ttgtctcatg tcatccaccg tgttcctcct cgaggattct ccgcaggcgc ttcctcccct
      781 ggctgagatg gacccggacg gttgcggcac tcgagccgat cacccgcgcg acctccccga
      841 tcgagtaccc gacgtaatag tggaggatga tcgcggcgcg ctgcttcggc gagagcctgc
      901 tgagcaaccc gaccagctca tcggccggct cgtccccggt cgtcaccggg ggcgaaagcc
      961 cgctcgcacg ccgtcgacgg tctttcagct ctccggctgc gatgcggaac gacacccgcc
     1021 acacccaccg cattggctca cggagcgccg cgcctctacc gagcgcctgc gcgaacgcct
     1081 cggccatcgc gtcgctcgcc acgtccgggt caccggcgta cgccaccaac gcccgccaga
     1141 gccgaccacc gtgctcgcgg tacagctcct cgagcaccag atccggcctg gacgtcacca
     1201 gcgcatcctg ccgcacaccc ctacaggggc gatccgagcc acgaacgtgt aggaggagac
     1261 cccggccgtg ttgtgttggc cggcccggtc tccgtcctgc tcgcggtggc cggtcaggcc
     1321 ggggcggcga ccttctcctc gtcgggcgag ggggcggact cctccaccga gaccttcggc
     1381 aggatccggt cgagccactt cggcagccac cagttccacc tgcccatcag ccgcatggtg
     1441 gccgggacca tgatcacgcg gatcagcgtg gcgtcgagca acaccgccac cgccaagccg
     1501 aagcccatcg tcttgatcgg cagcagccgg gcgaacgcga acgcaccgaa cacggtgacc
     1561 atgatcgccg cggcgctcgt gatgatccgc gcggtgtgct ccaggcccca gccgacggcc
     1621 tccgtgttgt cgcccgtgcg caggtattcc tcgcgcacgc gcgccagcag gaagacctcg
     1681 tagtccatgg acagaccgaa cagcgtggag aacaggaaca gcggcaggaa cgattcgatg
     1741 tgcccgttcg accggaaccc gagcagcccc tcccagtgac cctcctggaa cacgaacacc
     1801 agcacgccgt acgccgccac gaccgaggcg gtgttcatca ggatcgcctt cagcggcagc
     1861 agcaggctgc ggaagaagag catcagcagc acgaacgaca ggatcatcac cagcgcgacc
     1921 accgggatga tctgccgctg cacctcgtcg ttcaggtcca ggttcagacc gggggagccg
     1981 ccgagggcgg cgctcgcgcc ctccgggagc accggcggca ccagcgcgcg cagccgctcc
     2041 accgtcgcgt cgctctccgc ggtctgcggg ccgtgcgtcg tgacgatcgc cacgagcgtc
     2101 cggtcgccgt ccgccgccac cagctgggcg cgaacgtcgg cggagagcgg gctggccagg
     2161 aactgccgcg cctcgtcggc ggtggcgccc gggttgagcg ccagcagcgg cgtgagcacg
     2221 aagtcgaccc gcaccacgtc ggggtcctcg gcgatcgcgc cgaccgtcgc cagcagcccc
     2281 tcgaggccgt cgccggtcac cccgcgcggg tcggcgtaga ccatctggat cggcgagacg
     2341 gcgcccgcgc cgaacgcccc ggcggtgatg tcggcggcct gccgcgcggt ggagtcggcc
     2401 ggaaggatcg agggacccga ggaccccagc accaggttgc gggccgggaa ggccagaagg
     2461 ccgacgaccg ccaccgtgcc gatcaaggcc acccacggcc gggccatcac ggcgtgggcc
     2521 cagcgccgcc acaggcgagg cgagtcctcg gtccggcggc ggccgaagat gcgcagggag
     2581 tcgacccggc ggccgaccat gccgag
//
LOCUS       DASPWO010000477         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_148854, whole genome shotgun sequence.
ACCESSION   DASPWO010000477 DASPWO010000000
VERSION     DASPWO010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148854"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VEO00_10875"
     CDS             <1..455
                     /locus_tag="VEO00_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HYU58538.1"
                     /translation="TRVIGRSAGLLEVPIDPPGAEEIARRARGTPRVANRLLRRVRDF
                     AQVRGEGRIDRDVAMAALALFEVDEQGLDKLDVSILTTLVRKFGGGPVGLSTLAAAVG
                     EETETVEDVYEPYLLQLGFLQRTPRGRVATEAAFRHLGVSRHRDGLFT"
     gene            599..934
                     /gene="yajC"
                     /locus_tag="VEO00_10880"
     CDS             599..934
                     /gene="yajC"
                     /locus_tag="VEO00_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018301523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HYU58539.1"
                     /translation="MIAQSGSSGLATIIPLILMVAIFYFLLIRPQQRRMRQQRQLVES
                     LEVGDDILTIGGLFGRVTGMDDEALDVELAPGTTVRLLKSAVARRLTEPAPEEDLEDE
                     EEEEASESP"
     gene            931..1653
                     /locus_tag="VEO00_10885"
     CDS             931..1653
                     /locus_tag="VEO00_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYU58540.1"
                     /translation="MSKVARVPAAGEAAAEGAAEGAPITLETIQKDPTFSNFIVSADR
                     VMDGLGYTEHGFRHANLTGKIAFNLIARLERDRREAELASIAGYLHDLGNMVAREAHG
                     QVGAVLAHGVLRNRMAPDELALVMAGIGNHEEPEGAAAGPIPAAVILADKSDVHRSRV
                     RKADAIEFDIHDRVNYAATQSFLRVDSGARTITLELTIDTEISQVMEYFEIFLDRMVM
                     CRRAAEQLDCTFKLTINGATLL"
     gene            1688..>2108
                     /locus_tag="VEO00_10890"
     CDS             1688..>2108
                     /locus_tag="VEO00_10890"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58541.1"
                     /translation="MKNTRSLAASILFVAVLVALPALGFLSGSLKPLLGLDLVGGVDV
                     VLTAPEGTDREVMDRTLETIRNRVDAFGVAEPSIAIVGDRNIEVQIPGFQNEVGDAAR
                     QNRLLALIGSTARLEEREVLETIPRTADNYADVKLTCP"
BASE COUNT          314 a          755 c          754 g          285 t
ORIGIN      
        1 tgacgcgggt gatcgggcgc tcggccggcc tgctcgaggt cccgatcgac ccgcccgggg
       61 cggaggagat cgcccgccgc gcccggggga cgccgcgcgt ggcgaaccgc ctgctgcgcc
      121 gggtgcgcga cttcgcccag gtccgcggcg aggggcggat cgaccgggac gtggccatgg
      181 cggcgctggc gctgttcgag gtggacgagc agggcctcga caagctcgac gtctcgatcc
      241 tgaccacgct ggtgcgcaag ttcggcggcg gtccggtcgg gctctcgacg ctcgccgccg
      301 ccgtcggcga ggagaccgag accgtcgagg acgtctacga gccgtacctg ctgcagctcg
      361 ggttcctgca gcgcaccccg cgcggccgcg tggcgaccga ggccgccttc cgccacctcg
      421 gcgtgtcccg ccaccgggac ggcctgttca cctgagatgc acggggggct ccgccccccg
      481 tgtccccccc gaaagcgacg gacccggctt cgccggtccg gcccggcgga cctcctcgtg
      541 cgttgctagg atgagtggtc cgggccccgc gacgggccct tcctgaaggg acaccaacgt
      601 gatcgcgcag agcggatcga gcggcctcgc caccatcatc cccctgatcc tgatggtggc
      661 gatcttctac ttcctgctga tccggccgca gcagcgtcgc atgcgccagc agcgccagct
      721 ggtcgagagc ctcgaggtcg gcgacgacat cctgacgatc ggcggcctgt tcgggcgggt
      781 caccggcatg gacgacgagg cgctcgacgt cgagctcgcc cccggcacca ccgtgcgcct
      841 cttgaagagc gccgtggctc gccgcctcac cgagccggct cccgaggagg acctcgagga
      901 cgaggaggaa gaggaggcga gcgagtcgcc gtgagcaagg tcgcccgggt accggccgcc
      961 ggggaggcgg ccgccgaggg tgcggccgag ggcgcgccca taacgctcga gacgatccag
     1021 aaggacccga ccttctcgaa cttcatcgtg tcggcggacc gggtcatgga cggactcggg
     1081 tacaccgagc acgggttccg ccacgccaac ctgaccggga agatcgcctt caacctgatc
     1141 gcccgcctgg agcgcgaccg gcgggaggcc gagctcgcgt cgatcgccgg ctacctccac
     1201 gacctgggga acatggtggc ccgcgaggcg cacgggcagg tcggcgccgt gctcgcccac
     1261 ggcgtgctgc gcaatcgcat ggcgcccgac gagctggccc tggtgatggc ggggatcgga
     1321 aaccatgagg aaccggaggg ggcggcggcc ggccccatcc cggcggccgt gatcctggcg
     1381 gacaagtccg acgtgcaccg cagccgcgtg cgcaaggcgg acgcgatcga gttcgacatc
     1441 cacgaccgcg tgaactacgc cgccacgcag tcgttcctgc gagtagactc cggggcccgc
     1501 acgatcacgc tcgagctgac gatcgacacc gagatctccc aggtcatgga gtacttcgag
     1561 atcttcctgg accgcatggt gatgtgccgc cgcgccgcgg agcagctcga ctgcaccttc
     1621 aagctgacca tcaacggggc gacgctcctg tgagcgcacc ggtcgcccgc gatccggcga
     1681 ggccggcgtg aagaacaccc ggagcctcgc cgcctcgatc ctgttcgtgg cggtcctcgt
     1741 ggcgctcccg gccctcggct tcctgagcgg ttccctgaag ccgctcctcg gcctggacct
     1801 ggtcggtggc gtggacgtcg tcctgacggc gccggagggg accgaccggg aggtcatgga
     1861 ccgcacgctg gagacgatcc gcaaccgcgt cgacgcgttc ggcgtggccg agccgtcgat
     1921 cgccatcgtc ggcgaccgaa acatcgaggt ccagatcccg gggttccaga acgaggtcgg
     1981 cgacgcggcg cggcagaacc gcctgctggc gctgatcggc tcgaccgccc ggctggagga
     2041 gcgcgaggtc ctggagacga tcccgcgcac ggccgacaac tacgccgacg tcaagttgac
     2101 ctgccccc
//
LOCUS       DASPWO010000478         6824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_579391, whole genome shotgun sequence.
ACCESSION   DASPWO010000478 DASPWO010000000
VERSION     DASPWO010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579391"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VEO00_10895"
     CDS             <1..945
                     /locus_tag="VEO00_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYU58542.1"
                     /translation="CGTDLHEYLHGPLYTPAESLPQVIGHEFSAVVAEVGDGVGGLRP
                     GDRVAVLPHVFCGGCYYCVRGRQGLCADLLLTGVTWPWGGLAEQAVVPAYQAVRLPDG
                     VSFEQGAVLEPLATVEHGVERSGLRPGGSVLITGAGPIGQLAVLVCAAAGAGSIFVSE
                     PQPARRGLAERLGVTAAFDPAAGDVAAQVLERSDGLGVDCAIECSGSQPGLDACVASV
                     RRAGTVAVIAIHLGPRTVQPEGWVWRDLTLAGVWSFKFYDTPRILAQIAAGTLPVERI
                     VTSRIGIHDLVPAGIERLADPGGDQVKILVAPGDGADR"
     gene            942..1739
                     /locus_tag="VEO00_10900"
     CDS             942..1739
                     /locus_tag="VEO00_10900"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYU58543.1"
                     /translation="MSRFDGRTVLIVGGAQGMGRACAELFGAEGARLALFDIEADTLA
                     GTSADLQARGLPVEAITGDVSNAADVDAAVTRTVERFGGIDVLVHTAAIVELRPLLEF
                     PEDLWRRIVDVNLTGVFLTTRRAGREMAARGGGAIVVFASTNAFFAEELNVPYSATKG
                     ALVMFVRNAALDLARHGIRINAVDPGIIYTRLSAALIDDPVAGPEYLRRIPAGRYGTP
                     EDIAKVVLFLASDDAAYMTGEDVVVDGGATLGVALGIEDLGLEGTAE"
     gene            1759..2565
                     /locus_tag="VEO00_10905"
     CDS             1759..2565
                     /locus_tag="VEO00_10905"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYU58544.1"
                     /translation="MTPQGRLAGKVAIVTGGGSGIGRAVAKRFAAEGAAVAVADIAAG
                     NAEAVTAEILADGGRAFAMGVDVSDPQQVQALVDRAVREFGGLDVLMTAAGVLGFGSV
                     VETEPDEWSRVIGVNLTGTYLCARSAIPRMVERGGGSIVTVSSSTGAHDAAPGTAAYI
                     ASKGGVAMLTKAISVDHARQGIRANAIAPGPTDTPMLGGVMTPQELQAFGEAMPIGRL
                     AQPEELAAAALFLASDEASYVTGAVFAVDGGQTAKVGMTLDDLGTIARQG"
     gene            2593..4041
                     /locus_tag="VEO00_10910"
     CDS             2593..4041
                     /locus_tag="VEO00_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HYU58545.1"
                     /translation="MATETGRSGETGIGTELREGAVGLPGVLMQGIATIAPAFAILAS
                     FVFIVGFAGIVTPWAYVFGGALLALQALSAAQLARVFPSAGGWYTWIARALHPRAGFF
                     AGVFFSVWLPPVGALTLSLLGKTVLEPAIEEEYGVTIPWWIWVVAGLALVIWWAYAGI
                     VVSERLLIITGTIEIVLMVALAISALASPGPGGFSFAPLNPGNFSLASNLFLGVVFSI
                     FAFSGWESTGPLAEESRNPRRNVPIGLVGSVLILMLYFVFVTWGYLVGFGVDNVGQIS
                     FDIPFPVTTVAQRVWGGAWVLLLFALLNSAIAVSIACFNGGTRTWYGMGRSGVLPSAL
                     GKVAPSRQTPDNAIHLQVAVNVVAFLLVLIFGAENTFFSWANMITLGLITMYLLANIG
                     VATYYLTEARSQFNPLLHVVVPAIASAAGLVVVWKSYFSPFQHTGPVFWGLMSFIVLV
                     VITILALVVLRVRGQEDWLRKAQLVFEESGGH"
     gene            4062..5018
                     /locus_tag="VEO00_10915"
     CDS             4062..5018
                     /locus_tag="VEO00_10915"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HYU58546.1"
                     /translation="MEAETAVTGPAPLSAREFADIYDACRNWGRWGRDDQRGAINFIT
                     PDHVRAATATVRSGRTVACSWPLDTRAGPDNPKPVLHHMTMLPDIHLGDAGDLRFACD
                     FMGIEFHGDAHSHLDALCHVAYKGLLYNGVPVDRAVSSVGASMQTMDVAKDGIVGRGV
                     LLDIPRLRDTAWVEPGEAVYRDEIEAAEQAQGVRLEEGDILFFRTGHARKRLDDGPWD
                     SASRKAGFHATAMPLFHERRIAAIGFDGDGETVPSNCEGVIYPIHAIGVNAMGLYFLD
                     SLFFEDLAAACEQERRWDFLCVIAPLRLAAGTGSPVNPIAVF"
     gene            5117..6154
                     /gene="dmpG"
                     /locus_tag="VEO00_10920"
     CDS             5117..6154
                     /gene="dmpG"
                     /locus_tag="VEO00_10920"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HYU58547.1"
                     /translation="MNETIAPRVRLLDSTLRDGSHGIRQRYTPEQVKLIAGRLDEAGM
                     HSIGVGHGDGLGASSIQYMRSLHSDDELLSAARAAIRRARLAVTFQPGIGTKRHLERA
                     AELGADLVRIATHCTEADIAIQHIRLSRELGMDVHGDLMMPHMTSPGKIAEQARIMVD
                     AGATGVHIMDSAGALTMDDVKARIAAMLREFGGAAEAGIHAHQNLSLAVANTVVAFRE
                     GATLADACLAGAGAGAGNCQMEALIAVLEKLGIQTGIDLWTLEDIADSIVRPIMATPP
                     TVDRMTLTQGFAGVPGSFLHHASRAAERFGVDARDLLVEVGRRKSVSGQEDLLLEVAA
                     GLAGAERPAAG"
     gene            6185..>6824
                     /locus_tag="VEO00_10925"
     CDS             6185..>6824
                     /locus_tag="VEO00_10925"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYU58548.1"
                     /translation="MKAKRSGERGEVASAAELSTEVVHDRLRRAILRGELDPRVPISQ
                     VRLAATLGVSRTPLREALRMLQREGLIDSEPNRRVRVAELSMTDLEQLYAGRVLIEAL
                     AVRLTVPRFTAADMAALRAALADMNRRLEEREIDEWEAAHRRFHELLRRDAGERIGRI
                     ARDLADHAERYRRVYMAEPRIWSAAAEEHAAIVEACASGDGPLASVQLVRHLA"
BASE COUNT          922 a         2288 c         2588 g         1026 t
ORIGIN      
        1 tgcggcacgg acctgcacga gtacctgcac ggcccgctct acacgccggc cgagagcctg
       61 ccacaggtga tcgggcacga gttctccgcg gtggtcgcgg aggtcgggga cggcgtgggc
      121 ggcctgcggc cgggcgaccg tgtggcggtg ctgccgcacg tgttctgcgg cggctgctac
      181 tactgcgtcc gcgggcggca gggcctctgc gccgacctgc tgctcaccgg cgtgacctgg
      241 ccgtggggtg ggctcgccga gcaggccgtg gtgcccgcct accaggcggt caggctgccc
      301 gacggggtgt cgttcgaaca gggcgcggtc ctggaaccgc tggcgaccgt ggaacacggc
      361 gtggagcgct cgggcctgcg gcccggaggt tcggtcctga tcaccggcgc cggaccgatc
      421 gggcagctgg ccgtgctggt ctgcgcggcc gctggggccg gttcgatctt cgtctcggag
      481 ccgcagccgg caagacgcgg cctggccgag cgtctcgggg tgacggcggc cttcgacccc
      541 gccgccggcg acgtggccgc ccaggtgctc gagcgcagcg acggcctcgg ggtggactgc
      601 gcgatcgaat gctctggcag ccagccgggc ctcgacgcgt gcgtcgcctc ggtccggcgc
      661 gccggaacgg tcgcggtgat cgcgatccac ctcgggccgc gaacggtcca gccagagggc
      721 tgggtctggc gggacctgac gctcgccggg gtctggtcgt tcaagttcta cgacaccccg
      781 cggatcctcg cccagatcgc ggcggggacc ctgccggtgg agcggatcgt gaccagcagg
      841 atcggcatcc acgacctggt cccggccggg atcgagcggc tcgccgatcc cggcggcgac
      901 caggtcaaga tcctcgtcgc gcccggcgac ggagcggacc ggtgagccgc ttcgacggcc
      961 ggaccgtcct gatcgtcggc ggcgcgcagg gcatgggccg ggcgtgcgcc gaactcttcg
     1021 gcgcggaggg cgcgcggctg gcgctgttcg acatcgaggc ggacaccctc gccgggacct
     1081 cggccgacct ccaggcaaga ggcttgccag tggaggccat caccggggac gtgtcgaacg
     1141 ccgccgacgt cgacgccgcg gtcacccgca cggtcgagcg gttcggtggg atcgacgtcc
     1201 tggtgcacac cgcggcgatc gtggagctgc ggccgctgct ggagttcccc gaggacctct
     1261 ggcggcggat cgtcgacgtg aacctcacgg gggtcttcct gacgacccgc cgggccggcc
     1321 gggagatggc ggcgcggggc ggcggtgcga tcgtcgtgtt cgcatcgacc aacgcgttct
     1381 tcgctgagga gctgaacgtc ccctacagcg cgaccaaggg cgccctggtg atgttcgtcc
     1441 gcaacgccgc gctcgacctt gcgcggcacg ggatccggat caacgcggtc gaccccggga
     1501 tcatctacac gcggctgtcg gccgcgttga tcgatgatcc ggtcgcgggg cccgagtatc
     1561 tcaggcggat ccccgccggc cgctacggga cgccggagga catcgcaaag gtggtgctgt
     1621 tcctcgcctc cgacgacgcc gcctacatga ccggcgagga cgtggtggtc gacggcggag
     1681 cgacgctcgg cgtcgcgctc gggatcgagg acctcgggct ggagggcact gcggagtgaa
     1741 aggagggcag cgcaggggat gacgccgcag gggaggctcg cgggcaaggt cgccatcgtc
     1801 accggtggag gctccggcat cggccgggcg gtcgccaagc ggttcgccgc ggagggtgcg
     1861 gcggtcgccg tcgcggacat cgccgccggg aacgcggagg cggtcacggc cgagatcctg
     1921 gcggacggcg ggcgcgcgtt cgccatgggc gtggacgtgt ccgaccccca gcaggtgcag
     1981 gcgttggtgg atcgcgccgt gcgcgagttc ggcgggctcg acgtgctgat gaccgccgcc
     2041 ggcgtgcttg ggttcgggtc cgtggtcgag accgagcccg acgagtggag ccgggtgatc
     2101 ggggtcaacc tgaccggcac ctacctgtgc gcgcgcagcg cgatcccgag gatggtcgag
     2161 cggggcggtg gctcgatcgt cacggtctcc tcgtccaccg gcgcccacga cgcggcgccg
     2221 gggacggcgg cctacatcgc ctccaagggt ggggtggcga tgctcaccaa ggcgatcagc
     2281 gtcgaccacg ccaggcaggg gatccgggcg aacgcgatcg cgccggggcc gaccgacaca
     2341 ccgatgctcg gcggcgtcat gacgccgcag gagctgcagg cgttcggcga ggcgatgccc
     2401 atcggccgcc tggcgcagcc ggaggagctg gcggcggcgg cgttgttcct ggcctccgac
     2461 gaggcctcgt acgtgacggg tgcggtgttc gcggtcgacg gggggcagac ggcgaaggtc
     2521 ggcatgaccc tcgacgacct cggaacgatc gcacggcaag ggtaacggca ggcaaccgga
     2581 agggagcaca cggtggcaac cgagactggt cggtctggcg agacgggcat cgggaccgag
     2641 ctgcgcgagg gcgcggtcgg cctgcccggc gtgctgatgc agggcatcgc gacgatcgct
     2701 ccggcgttcg cgatcctcgc gtcgttcgtg ttcatcgtgg gcttcgcagg catcgtcacc
     2761 ccatgggcct acgtgttcgg tggggcgctg ctggcgctgc aggcgctgtc ggccgcgcag
     2821 ctggccaggg tgttcccgtc ggccggtggc tggtacacct ggatcgcccg cgcgctgcat
     2881 ccgcgggccg gcttcttcgc cggggtgttc ttctcggtgt ggctgccgcc ggtcggcgcg
     2941 ctgaccctgt cgctgctggg caagaccgtc ctggagccgg cgatcgagga ggagtacggc
     3001 gtcacgatcc cctggtggat ctgggtcgtc gccgggctgg cgctggtgat ctggtgggcg
     3061 tacgccggca tcgtggtctc cgagcggctg ctgatcatca ccgggacgat cgagatcgtg
     3121 ctgatggtcg ccctggccat cagcgcgctc gccagcccgg gacccggcgg cttcagcttc
     3181 gcgccgctca acccggggaa cttctccctg gcctccaacc tgttcctcgg ggtggtgttc
     3241 tcgatctttg ccttcagcgg ctgggagtcc accggccccc tggccgagga gagcaggaac
     3301 ccgcgccgca acgtcccgat cggcctggtg ggctcggtgc tgatcctgat gctctacttc
     3361 gtgttcgtca cctggggcta cctcgtcggc ttcggcgtcg acaacgtcgg ccagatcagc
     3421 ttcgacatcc ccttccccgt cacgaccgtc gcgcagcgcg tgtggggcgg cgcctgggtg
     3481 ctgctgctgt tcgcgctgct gaactccgcg atcgcggtct ccatcgcctg cttcaacggc
     3541 ggcacccgca cctggtacgg catgggccgg tccggcgtgc tgccgtcggc gctgggcaag
     3601 gtggcgccga gcaggcagac gccggacaac gccatccacc tccaggtcgc cgtgaatgtg
     3661 gtggccttcc tgctggtgct gatcttcggg gccgagaaca cgttcttcag ctgggccaac
     3721 atgatcacgc tgggcctgat caccatgtac ctgctggcga acatcggggt ggcgacgtat
     3781 tacctgaccg aggccaggag ccagttcaat ccgctcctgc acgtcgtcgt cccggccatc
     3841 gcgtcggcgg cagggctcgt cgtcgtctgg aagtcgtact tctcgccgtt ccagcacacc
     3901 ggcccggtgt tctgggggct gatgtccttc atcgtgctgg tcgtcatcac gatcctggcg
     3961 ctggtcgtcc tgcgggtgcg cggccaggag gactggctgc gcaaggcgca gctcgtgttc
     4021 gaggagtccg ggggtcacta gggccgggga gggcgcgtcg catggaagcg gaaacggcgg
     4081 tgaccggccc ggcgccgttg tccgcgcgcg agttcgcgga catctacgac gcctgcagga
     4141 actggggacg ctgggggcgg gacgaccagc gcggggcgat caacttcatc acgcccgacc
     4201 acgtacgcgc ggcgacggcg acggtccggt caggccggac cgtcgcctgc tcgtggccgc
     4261 tcgataccag ggccggtccc gacaacccca agccggtcct gcaccacatg acgatgcttc
     4321 cggacatcca cctcggtgac gccggcgacc tgcggttcgc gtgcgacttc atggggatcg
     4381 agttccatgg ggacgcgcac tcccacctcg acgcgctgtg ccatgtcgcc tacaaggggc
     4441 tgctgtacaa cggcgtcccg gtggaccggg cggtctcctc cgtcggcgcc tcgatgcaga
     4501 ccatggatgt cgccaaggac gggatcgtcg ggcggggggt gctgctcgac atcccccgcc
     4561 tgcgcgacac ggcgtgggtc gaaccgggcg aggccgtgta ccgggacgag atcgaggccg
     4621 cggagcaggc gcagggcgtt cgtctggagg agggcgacat cctgttcttc cggaccggcc
     4681 acgcgcgcaa gcggctggac gacgggccgt gggactcggc gagccggaag gcggggttcc
     4741 atgcgaccgc gatgcccctg ttccacgagc ggaggatcgc ggcgatcggc ttcgacggcg
     4801 acggcgagac ggtcccgagc aactgcgagg gggtgatcta cccgatccac gcgatcgggg
     4861 tgaacgcgat ggggctgtat ttcctcgaca gcctgttctt cgaggatctc gccgccgcgt
     4921 gcgaacagga gcgccgctgg gatttcctgt gcgtgatcgc gccgctgcgc ttggcggcgg
     4981 gcacgggatc gccggtgaat ccgatcgcgg tgttctgacg caggggacta tccacggccg
     5041 acacaggcaa cctcgacatc atgacggcgg tggccgtccg ggtcgccgaa cgatacgcgg
     5101 agaggttcgc gacgacatga acgagaccat tgcgccacgg gtccggctgc tggactcgac
     5161 gctgcgcgac gggtcgcacg ggatccgcca gcgctatacg cccgagcagg tcaagctgat
     5221 cgcgggcagg ctggacgagg cgggcatgca ctcgatcggc gtcgggcatg gcgacgggct
     5281 cggcgcctcg tcgatccagt acatgcgctc gctccacagc gacgacgagc tgctgtcggc
     5341 ggcacgcgcg gcgatcaggc gcgcgcggct ggcggtgacg ttccagccgg gcatcggcac
     5401 caaacggcat ctcgagcgcg cggccgagct tggcgcggac ctggtgcgga tcgcgacgca
     5461 ctgcaccgag gcggacatcg cgatccagca catccggctg tcccgcgagc tcgggatgga
     5521 cgtgcacggg gacctgatga tgccgcacat gacctcgccc gggaagatcg cggagcaggc
     5581 ccggatcatg gtcgacgccg gcgcgaccgg ggtccacatc atggactccg ccggcgcgct
     5641 cacgatggac gacgtgaagg cccggatcgc cgcgatgctc cgggaattcg gcggcgcggc
     5701 cgaggccggg atccacgcgc accagaatct ctcgcttgcg gtcgccaaca cggtggtggc
     5761 gttccgggag ggcgccacgc tcgcggacgc atgcctggcc ggcgccggcg caggcgccgg
     5821 gaactgccag atggaggcgc tcatcgccgt gctcgagaag ctcggcatcc agacgggcat
     5881 cgacctgtgg acgctcgagg acatcgccga ctcgatcgtc cggccgatca tggcgacacc
     5941 gccgacggtc gaccggatga ccctcacgca ggggttcgcc ggcgtgccgg gcagcttcct
     6001 gcatcatgcc tcccgggccg ccgagcggtt cggcgtggac gcccgcgacc tcctggtcga
     6061 ggtcggccgg cgcaagtcgg tcagcggcca ggaggacctg ctgctcgagg tcgccgcggg
     6121 cctggccggg gccgagcggc ccgcggcggg ctgagcgcca gtggcgcggg tcggcttgcc
     6181 tggcatgaag gcgaagcggt ccggtgagcg cggcgaggtg gcgtccgcgg ccgagctgtc
     6241 gacggaggtc gtccacgacc ggttgcgacg ggcaatcctg cgcggtgagc tggacccgcg
     6301 cgtgccgatc tcgcaggtcc ggctcgccgc gacgctgggg gtgagccgca ccccgctgcg
     6361 ggaggcgctg cgcatgctcc agcgtgaggg gctgatcgac tcggagccga accggcgcgt
     6421 ccgggtcgcc gagctgtcca tgaccgacct cgagcagctc tacgccggcc gggtgctgat
     6481 cgaggcgctc gcggtccggc tgaccgtgcc gagattcacc gccgccgaca tggccgcgtt
     6541 gagggcggcg ctggcggaca tgaaccggcg gctcgaggag cgcgagatcg acgaatggga
     6601 ggccgcgcac cggcgcttcc acgagctgct gcgcagggac gccggcgagc ggatcgggcg
     6661 gatcgcccgc gacctcgcgg atcacgccga gcgctaccgg cgcgtctaca tggccgagcc
     6721 tcggatctgg tccgctgccg ccgaggagca cgccgccatc gtcgaggcgt gcgcctccgg
     6781 ggacggtccg ttggcgagcg tgcagttggt ccgtcatctc gccc
//
LOCUS       DASPWO010000479         3123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1252085, whole genome shotgun sequence.
ACCESSION   DASPWO010000479 DASPWO010000000
VERSION     DASPWO010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252085"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1719
                     /locus_tag="VEO00_10930"
     CDS             <1..1719
                     /locus_tag="VEO00_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58549.1"
                     /translation="GGASVSGPEGAEGALPAPPPVPRPPLPPAPPPEPPTGNRSTERT
                     GPDDTTVGVGAQRLVAVLIMVVTLLGAVFAFLQTQAGNRESSANRSAQAAAIQATTDI
                     VDGNRAINQDDLAWELQDDQGWFRFYLEDDTGPAASFARALARAYDAAQRDLKDLSVV
                     ERTGTYKLPDDEIDWARFYEDQYRASYSSTEFQRAHAEERDGWSAKGSQYVTVITVLA
                     MALFLLGLTLTVPAAARRPFLVTGTIVAVVGTAWGATVWLRPVERPSAEAIEAYVDGQ
                     VLLNAGSDTEDFQQAVDRFGAAIRARPDYVDAYVSRGNAYFELDFLNPDGPQGSMEAV
                     ADFQRAIDLGLNDVLSWGNLGAAQWWLGEYDAALESTRTAFELDRSDITVSLNYAEVL
                     LTAGGLDDPGYRSQLRHVRGLLERAPTWLRDSKMESFYEAVDLAVRYRPDLAELESAF
                     KEAMIRIHHEIDLNNELYGRPRPEPVDATITDLQFTLSPDHTTLDIDFDYAGVAEGQR
                     WLYRTYVDGLWQEDWSIEPEPWPFDVPDGGVTITLTGSFDKDEIVRTEIFLEGNLLAA
                     GELTVP"
     gene            complement(1790..2017)
                     /locus_tag="VEO00_10935"
     CDS             complement(1790..2017)
                     /locus_tag="VEO00_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58550.1"
                     /translation="MTWSMVTERGEVVSEIRSCGDVLEALARLEPAGENILTELLPWA
                     RSSSGGTPRRRCMMVAFVLRDGAVQQIVSPA"
     gene            2238..3041
                     /locus_tag="VEO00_10940"
     CDS             2238..3041
                     /locus_tag="VEO00_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYU58551.1"
                     /translation="MDGDWMSYDAVAELYERHHARRTALVARDLVATMAIEPGMRVLD
                     VGTGTGVTAAAAHEAVGDGGLVVGIDPSLGMLGRGLAAGRSVRFAAGEALDLPFRNGT
                     FDAETATFVLPQFAKQDTALFDLHRVLRPGGTLGVAVWGPSNDEFDRTWNDIAERYLG
                     TDILRDALARMPWEDRLGNPAVLSEVLHRAEFRKIRIEPREYRFEWTRDDYIEGRTTH
                     RVSRMTRNLLGEELWGRFLDDIRRTYAERFPERFGDTKDVLLAVSVRES"
BASE COUNT          464 a         1109 c         1112 g          438 t
ORIGIN      
        1 ggtggagctt ctgtgagcgg gcccgagggc gccgagggcg ccctgccggc gccgccaccg
       61 gtaccgcggc cgcctctgcc acccgccccg ccgccggagc cacccacggg gaaccggagc
      121 accgagcgca ccgggcccga cgacacgacc gtcggggtgg gagcccagcg cctggtggcc
      181 gtgctgatca tggtggtgac cttgctcggt gcggtgttcg cgttcctgca gacccaggcc
      241 ggcaaccgcg agtcgagcgc caaccgcagc gcgcaggcgg cagccatcca ggcgaccacc
      301 gacatcgtcg atgggaaccg cgccatcaat caggacgacc tggcctggga gcttcaggac
      361 gaccagggtt ggttccggtt ctacctggag gacgacaccg gccccgccgc ctcctttgcc
      421 cgtgcgctgg cgcgggcgta tgacgcggcc cagcgggacc tgaaggatct ctccgtcgtg
      481 gagcggaccg gtacctacaa gctcccggac gacgagatcg actgggcgcg cttctacgag
      541 gatcagtacc gcgcctcgta ctcctcgacg gagttccagc gcgcccacgc cgaggagcgg
      601 gacggctggt cggccaaggg ttcgcagtac gtgacggtga tcaccgtact ggccatggcc
      661 ctgttcctcc tcggcctgac gctgacggtc ccggccgccg cccgccggcc gttcctcgtc
      721 accggaacga tcgttgcggt ggtggggacg gcctggggag cgaccgtatg gctgcggccg
      781 gtcgagcggc cgtcggccga ggcgatcgag gcgtacgtcg acggccaggt cctcctgaac
      841 gccggttcgg acacggagga cttccaacag gcggtcgatc ggttcggcgc cgccatccgg
      901 gcgcggccgg actacgtcga tgcctacgtc agccgaggga acgcctactt cgagctggac
      961 ttcctcaacc cggacggccc tcagggctcg atggaggccg tggccgactt ccagagggcc
     1021 atcgacctcg gcctgaacga cgtgctctcc tgggggaacc tgggcgcggc gcagtggtgg
     1081 ctcggcgagt acgacgccgc gctggagtcg acccgcacgg cgttcgagct cgaccggagc
     1141 gacatcacgg tgagcctgaa ctacgccgag gtcctcctga ccgccggcgg gctcgacgac
     1201 cccgggtacc ggtcgcagct ccggcacgtc cgcggcctcc tggagcgggc cccgacctgg
     1261 ctgcgcgact cgaagatgga gagcttctac gaggcggtgg acctggcggt gcgctaccgg
     1321 ccggacctgg ccgaactgga gagtgccttc aaggaggcca tgatccggat ccaccacgag
     1381 atcgacctga acaacgagct gtacggccgg ccccggcccg agcccgttga cgccacaatc
     1441 accgacttgc agttcacgct ctcgccggac cacaccacgc tggacatcga cttcgactac
     1501 gccggggtgg ccgagggcca gcggtggctc taccggacgt acgtcgacgg gctctggcag
     1561 gaggactggt cgatcgagcc agaaccctgg ccgttcgacg tcccggacgg cggcgtgacc
     1621 atcacgctga ccggctcgtt cgataaggac gagatcgttc ggaccgagat cttcctcgag
     1681 ggcaacctgc tcgccgccgg cgagctcacc gttccgtaac tgcccacaag ccggcgcggc
     1741 ttcgttcgga agcgccagaa cgggcgatcc ggcggagcgg acccgcgggt caggcggggc
     1801 tgacgatctg ctgaacggcc ccgtcccgga ggacgaacgc gaccatcatg cagcgtcttc
     1861 tcggcgtgcc gcccgaggat gatctcgccc acggcaacag ttcggtgagg atgttctccc
     1921 ccgcgggttc gagacgggcc agcgcttcga ggacgtcccc gcagctgcgg atctccgaga
     1981 ccacctcgcc tcgttcggtg accatgctcc aggtcacgtc gtcgagggct ccggtggcga
     2041 aaccggaagc gctcgtcgtg ggcgccagcg ggtcgcggcc gcaggtggag agtacgcctc
     2101 cggcggaggc cagggctgcg gtcgcgccgg ccccccggag gaggcggcgc cgcccgaggt
     2161 cgagtcaccc gctcgccacg gcagcggaag gaggtcgacg ttccctattc cagcccgggc
     2221 ccatagagtg atccgccgtg gatggggact ggatgtcgta cgacgccgtc gccgagttgt
     2281 acgagcggca ccacgcgcgg cgcacggccc tggtggcccg ggacctcgtg gcgacgatgg
     2341 ccatcgagcc gggcatgcgc gtgctcgacg tcgggaccgg caccggggtg accgcggccg
     2401 cggcgcacga ggccgtcggc gacggcggcc tggtcgtcgg gatcgatccg tcgctcggca
     2461 tgctcgggcg ggggctggcg gccggacggt ccgtgcgctt cgccgccggc gaggcgctcg
     2521 acctcccgtt ccgaaacggc acgttcgacg ccgagaccgc gacgttcgtg ctcccccagt
     2581 tcgccaagca ggacaccgcg ctgttcgacc tgcaccgcgt gctgcgcccc ggcgggacgc
     2641 tcggcgtcgc ggtgtggggc ccgtcgaacg acgagttcga ccggacctgg aacgacatcg
     2701 cggagcgcta cctgggcacc gacatcctgc gcgacgccct ggcccgcatg ccctgggagg
     2761 atcgcctcgg caacccggcc gtgctctccg aggtgctgca ccgggcggag ttccgcaaga
     2821 tacggatcga gccccgcgag taccggttcg agtggacccg ggacgactac atcgagggcc
     2881 gcaccacgca ccgggtctcg cggatgacgc ggaacctgct cggcgaggag ctctgggggc
     2941 gcttcctgga cgacatccgc cggacctacg ccgagcggtt cccggaacgg ttcggcgaca
     3001 cgaaggacgt gttgctggcc gtctcggtcc gcgaatcctg accgtcctcc gctgcgggca
     3061 ggtgcagccg cagcagctcc ccgccaccct ccgccggctc ggcggagacc cggccgccgc
     3121 tcg
//
LOCUS       DASPWO010000480         8856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_917732, whole genome shotgun sequence.
ACCESSION   DASPWO010000480 DASPWO010000000
VERSION     DASPWO010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917732"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3887
                     /locus_tag="VEO00_10945"
     CDS             <1..3887
                     /locus_tag="VEO00_10945"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYU58552.1"
                     /translation="VIAGDDATSDHLLEWIGAGFLASGAAATDEVVEDAATPFDRRRH
                     GLIVGMGGAALVLERADVARERGIRPICQVLGAVTANSAFHGSRLDVHHIASVMEGLV
                     SGAEARWGIRREEIAPELVFVSHETYTPARGGSASAEVEALRRVFGDTTDRIVVANTK
                     GFTGHPMGVGIEDVVAVKSLETGLVPPVPNFKEVDPELGTLNLSRGGAYPVRYALRLA
                     AGFGSQISMSLLRWVPTPDGSRPAPQSLGPEGRVADASARTAWLERIAGRPEAELEVV
                     QRRLRVAEPPRADLGAPAVVEAAATPEPEIVPVPEAAVPVTETAPAPEPVAVPAEPVA
                     VPAEPAPVPAEPAPVAPPEEEVRERVLALVAEQTGYPADMLELDLDLEADLGIDTVKQ
                     AEIFVALRREYGIARDESLKLRDFPTLEHVIAFARDRAGSEALMALEETATAGPPAET
                     AVAAAQAPVDRERVTAGLQVADAVPRRVPVPVLRPPIERCKDTGVVLSDGSRVLVMAD
                     AGGAAKALASRLEELGADVLLVDDAPETDALSERIATWTAGEPVAGVVWLPALDRAEP
                     IARLDLPAWREALRRRVKLLYATMRQLTADGSDPFLVAATRLGGWHGYEAAGATDTLG
                     GAVVGFAKAYRRERPGATVKAVDVEVEAPAEEVADRLLEETLRDPGAVEIGYREGLRF
                     AIGLAERPAEAPAAELALGPRTVFAVTGAAGGIVSEIVADLSAATGATFHLLDLAPEP
                     DPGDPDLAAFDADREGLKPALAERIRARGERATPALVERELAKLERARAGLAAIEAVR
                     TAGGAAVYHQVDLTDAEGVQEVVDRIRDESGRIDVLIHAAGLEISHALADKEPREFDL
                     VFDVKADGLFNLLHALGEDPPGATVVFSSVAGRFGNAGQTDYSAANDLLCKVVSNLRR
                     SGSGVRGIAIDWTAWAGIGMASRGSIPKIMEAAGIDMLEPAAGIPVVRRELLGATDGE
                     VVIGGRLGVLTEELDESGGLTPGAEAGSGPLPVVPAAMGVYTGLRVEIELDPAAEPFL
                     DHHRIDGTAVLPGVMGIEAFAEAARWAAPGWVVAAIDDVEFLAPCKFYRDEPRTLTVE
                     ASVTPDGDDLVATCRLVGVRELPNRPEPQVTTHFTGRVRLARGVGDLGSEAPPARRGP
                     GVGAEDVYRVFFHGPAYRVLERVWPSEASATGLMAGGLPPDLSRPGQASAAPRLIELA
                     FQTAGAWEIAARDRFGLPQSVGRIRVATGANRPAGRPVALVRSGQEPGAIDATVVDGD
                     GAVLVRVEGYRAVELPGALDPRSAAPFRAGVA"
     gene            3887..8083
                     /locus_tag="VEO00_10950"
     CDS             3887..8083
                     /locus_tag="VEO00_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYU58553.1"
                     /translation="MGTDRTLAVALVASAIRALDEDEAEERARFLASAARGRPVVSDE
                     YGRAVALREGGVSYAFRVSRTGPTDYTVERAGQRLAAGWESFGGAVGRLSLAAGVWPV
                     TWPEADGGAIRVAGRAFDVARDDRGLVRAPAPAVVVAVSVEAGQRVEAGAPLVVLEAM
                     KMEMTVAAPHDGWVRRTLVGPNEQVDAGAALLQLAPAEVAGAEGEPVGFDLLAGPQGR
                     TDHPCALVLDGLRLLMLGFDVDPAIARTLGARWSASCADDGEIAEQILRGEEEVLQSF
                     ADVVALSQRTVGLPDEDGETMRANGEEFLTYLRYLDAGHPALSGAFLDALRRALGHHG
                     VASLERSTALEEALLRLYRSQHRTEHLVPAVLAILDRRLERIEQLRQAVGPEFQALLD
                     RLVAVTRGRHLGVSDLAREVRYRYFDQPLIEEVRRSAYARAEDDLHALAASPSGPDRE
                     ARIRALVECPQPLKRLLTERMQAAAGEARAAMLEALARRYYRIREVEDARTRLVDGRH
                     VFTARYPHDGATIHLVATHGAWPELARAWSVAIGEATAAPAGDEVVIDLYVWRGGPPE
                     AAETTSERIAAVLDAAPIDRPIRRVVVALSGPGASLEAAAMEHFTFRPGPEGYREERP
                     YRGLHPMMGKRLDLWRLSRFTIDRLPSVEDVYLFHGVARENPKDERLFALAEVRDLTV
                     ARDGDGRLVGVPQLELMLTEALEAIRLYQSHRPPGKRLQWNRVLLNAWPPVDLPIGEL
                     YGIVHSLAPLAEGLGLQKVVVRARVLDEVTGEVHDRVLHISNPAGRGLALRFDEPSDV
                     PIPPLSPYRQKVVQTRQRGLTYPYEIVRMLTPPPGTPADFPPGEFLEHDLGEDGSLVA
                     VDRSHGENAANVVVGVITNHTDAHPDGMRRVAILGDPSRSLGSLAEPECRRIIAALDL
                     AERMGVPLEWFAVSAGAKIAMDSGTENMDWIGRVLRRLIEFTQGGGEVNVLVCGINVG
                     AQPYWNAEATMLMHTKGILVMTPESAMVLTGKQALDYSGGVSAEDNVGIGGYDRVMGP
                     NGQAQYWAADLDEACRILFRHYEHTYVQPGERFPRRVPTTDPIDRDVRAFPHRASGEG
                     GFATIGDVFSERANPERKKPFDIRSVMRAVVDRDHAVLERWGGMRDAEIAVVWDAHLG
                     GIPVCLLGIESRPLPRHEVAPADGPDYWTSGTLFPLSSKKVARAVNAASGVRPLVVLA
                     NLSGFDGSPESLRNLQLEYGAEIGRAVVNFDGPIVFCVVSRYHGGAFVVFSRTLNEHI
                     EIAAVEGSFASVIGGAPAAAVVFAREVDERTKSDPRVASLAQELAEVAPGERGRLLAA
                     LERATASVRSEKLGEVAAEFDHIHTIYRALEVGSVDRIIAPSALRPYLIDAVERGIAR
                     VLGRAEGGTERPSELVESRP"
     gene            8080..8769
                     /locus_tag="VEO00_10955"
     CDS             8080..8769
                     /locus_tag="VEO00_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HYU58554.1"
                     /translation="MTVGATVRVLLQRADEVPVDDGWLAPEERAIMDGLRVPKRRADW
                     RLGRWTAKRAVAETLGTAAGPPADLRILAGPDGAPEAFVGWRPLPVAISISHRGGLAA
                     CAVAPANAALGCDLEVVEARSAGFVADFFTEDERALVEAAPPARRHLLAALVWSAKES
                     ALKALRTGLRSDTRSVEVSVEPGTAGAWSPLAVRDRSADRRLRGWWRAVDEAVLTVVA
                     DPPVGRPSAIA"
BASE COUNT         1093 a         3145 c         3464 g         1154 t
ORIGIN      
        1 tcgtgatcgc cggtgacgac gcgacctcgg accacctgct ggagtggatc ggtgccgggt
       61 tcctcgcctc cggcgcggcg gccaccgacg aggtggtcga ggacgctgcc acgccgttcg
      121 accggcgccg ccacggcctg atcgtgggga tgggcggggc cgcgttggtc ctcgagcgcg
      181 ccgacgtcgc gcgcgagcgc gggatccggc cgatctgcca ggtcctcggc gcggtcaccg
      241 cgaacagcgc ctttcacggc agccggctgg acgtccacca catcgcgtcg gtgatggagg
      301 ggcttgtctc cggagcggaa gcgcggtggg ggattcgccg cgaggagatc gcccccgagc
      361 tggtcttcgt ctcgcacgag acctacacgc cggcccgcgg cggcagcgcc tccgcggagg
      421 tcgaggccct gcgccgcgtc ttcggcgaca ccaccgaccg gatcgtcgtg gcgaacacga
      481 agggcttcac cggccacccg atgggcgtcg ggatcgagga cgtcgtggcg gtgaagtcgc
      541 tcgagaccgg gctggttccg ccggtgccga acttcaagga ggtcgacccc gagctgggga
      601 ccctgaacct gtcgcgggga ggggcctatc ccgtgcgcta cgcgctgcgg ctggccgcgg
      661 gcttcggctc gcagatcagc atgagcctgc tgcgctgggt gccgacgccg gacgggagcc
      721 gcccggcgcc gcagtcgctc ggccccgagg gacgcgtcgc ggacgcctcg gcccggacgg
      781 cctggctgga gcggatcgcc ggccgcccgg aggccgagct cgaggtggtg cagcggcggc
      841 tgcgcgtcgc cgagccaccg cgcgccgatc tcggcgcgcc ggccgtggtg gaggcggccg
      901 cgaccccgga gccggagatc gtgccggtgc ccgaggcagc agtccccgtg accgagaccg
      961 cgccggctcc ggagcccgtg gccgttcccg cggagcccgt ggccgttccc gcggagcccg
     1021 cgcccgttcc cgcggagccc gcacccgtcg cgccccccga ggaggaggtg cgcgagcgcg
     1081 tcctggccct ggtcgccgag cagaccggct acccggccga catgctcgag ctcgacctgg
     1141 acctcgaggc cgacctcggc atcgacaccg tgaagcaggc ggagatcttc gtcgccctgc
     1201 gccgggagta cggcatcgcc cgcgacgaga gcctgaagct gcgcgacttc ccgaccctcg
     1261 agcacgtcat cgcgttcgcc cgcgaccggg ccggctccga ggccctcatg gccctggagg
     1321 agaccgccac ggccggtccc ccggcggaga ccgccgtggc cgccgcccag gcgccggtcg
     1381 accgggaacg ggtgaccgca ggcctgcagg tggccgacgc cgtcccccgg cgagttccgg
     1441 tgcccgtcct gcgcccgccg atcgagcggt gcaaggacac cggcgtcgtc ctctccgacg
     1501 gctcccgcgt cctggtgatg gccgacgccg gcggcgccgc gaaggcgctc gcgtcccggc
     1561 tcgaggaact cggcgccgac gttctgctgg tggacgacgc gcccgagacg gacgcgctct
     1621 ccgagcggat cgcgacgtgg acggccgggg agccggtggc cggcgtggtc tggctgcccg
     1681 ccctcgaccg ggcggagccg atcgcccggt tggacctccc ggcctggcgc gaggcgctgc
     1741 gccgccgggt gaagctcctc tacgccacga tgcggcagct gaccgccgat ggttccgacc
     1801 cgttcctcgt ggcggcgacg cggctgggag ggtggcacgg gtacgaggcg gctggtgcga
     1861 cggacaccct cggtggggcg gtcgtcgggt tcgcgaaggc ctatcggcgc gagcggcccg
     1921 gcgcgacggt gaaggccgtc gatgtcgagg tcgaggcgcc ggccgaggag gtcgccgaca
     1981 ggctgctcga ggagacgctg cgcgacccgg gagccgtcga gatcggctac cgggagggcc
     2041 tgcgcttcgc gatcgggctc gcggaacggc ccgccgaggc gcccgccgcc gagctcgcgc
     2101 tcgggccgcg gaccgtcttc gccgtgaccg gcgccgccgg cggcatcgtg tcggagatcg
     2161 tggcggacct ctccgcggcc acgggagcga cgttccatct gcttgacctg gcccccgagc
     2221 ccgatccggg cgaccccgac ctcgcggcgt tcgacgccga ccgggagggg ctgaagcccg
     2281 cgctcgccga gcggatccgc gcccggggcg agcgggccac ccctgccctg gtcgagcgag
     2341 agctggcgaa gctggagcgg gcgcgggcgg ggctcgccgc gatcgaggcc gtccggacgg
     2401 ccggcggagc ggccgtctat caccaggtcg acctgaccga cgccgaggga gtgcaggagg
     2461 tcgtggaccg gatccgcgac gagagcggac ggatcgacgt gctgatccac gcggccggcc
     2521 tggagatcag ccacgcgctg gccgacaagg agccgcggga gttcgacctg gtcttcgacg
     2581 tcaaggccga cgggctgttc aacctgctcc acgccctcgg cgaggacccg cccggcgcga
     2641 ccgtggtgtt cagctccgtg gccggccggt tcggcaacgc ggggcagacc gactacagcg
     2701 cggcgaacga cctgctgtgc aaggtcgtgt cgaacctgcg ccggagcggg agcggcgtgc
     2761 gcgggatcgc catcgactgg accgcctggg ccgggatcgg gatggcatcc cgaggctcga
     2821 tcccgaagat catggaggcg gccgggatcg acatgctcga gccggcggca gggatcccgg
     2881 tggtccgccg cgagctgctc ggcgcgaccg atggcgaggt ggtgatcggc ggccggctcg
     2941 gggtcctgac cgaggagctc gacgagtccg gcggcctgac gccgggcgcc gaggccggca
     3001 gcggtccact tccggtcgtc cccgcggcga tgggggtgta caccggcctg cgcgtggaga
     3061 tcgagctcga tcccgccgcc gagccgttcc tggaccacca ccggatcgac ggaaccgccg
     3121 tgctcccggg ggtgatgggc atcgaggcgt tcgccgaggc cgcacgctgg gcggcgcccg
     3181 ggtgggtcgt cgcggcgatc gatgacgtcg agttcctggc cccctgcaag ttctaccggg
     3241 acgagccgcg cacgcttacg gtcgaggcgt cggtcacgcc ggacggggac gacctggtcg
     3301 cgacctgccg cctggtcggc gtccgggagc tgccgaaccg gccggagccc caggtgacga
     3361 cccacttcac cgggcgggtc cggctggcga ggggcgtcgg cgatctcggg tcggaggcgc
     3421 cgccggcgcg acgcgggccg ggcgtcgggg ccgaggacgt gtacagggtg ttcttccacg
     3481 gcccggccta ccgcgtgctc gagcgtgtct ggccgtcgga ggcctccgcg accggcctga
     3541 tggcgggcgg cctgccgccg gacctgtcgc gtccgggcca ggcatccgcc gccccgcggc
     3601 tgatcgagct cgcgttccag accgccggcg cctgggagat cgccgcccgg gaccggttcg
     3661 ggctcccgca gtcggtcggg cgcatccgcg tggcgaccgg cgcgaaccgg cccgccggcc
     3721 gcccggtcgc cctggtccgg tccggacagg agccgggcgc gatcgacgcc acggtcgtgg
     3781 acggggacgg cgccgtcctg gtgcgcgtcg agggctaccg cgcggtggag ctgcccggcg
     3841 cgctcgaccc gcggtccgcc gccccgttcc gggcgggggt ggcctgatgg ggaccgaccg
     3901 gacgctggcc gtcgccctgg tcgcctcggc gatccgggcg ctcgacgagg atgaagccga
     3961 ggagcgggcc cggttcctgg cgtcggccgc gcgcgggcgg cccgtggtgt cggacgagta
     4021 cggacgggcc gtcgcgctgc gcgagggggg cgtgagctac gcgttccgcg tcagtcgcac
     4081 gggtccgacc gactacacgg tcgagcgcgc cgggcagcgg ctcgcggcgg ggtgggaatc
     4141 gttcggcggc gccgtgggcc ggctgagcct cgcggcgggc gtctggccgg tgacctggcc
     4201 ggaggcggac ggcggggcga tccgcgtggc cggacgcgcc ttcgacgtcg cgcgggacga
     4261 ccgcgggctg gtgcgagcgc cggcgccggc cgtggtggtc gccgtgtccg tcgaggcggg
     4321 gcagcgggtc gaggcgggcg cgccgctcgt ggtcctcgag gcgatgaaga tggagatgac
     4381 ggtcgcggcg ccgcacgatg ggtgggtgcg gcgcacgctc gtcggcccga acgagcaggt
     4441 cgacgcggga gcggcgctgc tgcagctcgc gccggccgag gtcgccgggg ccgaagggga
     4501 gccggtgggg ttcgacctgc tcgccggccc tcaaggaaga acggaccacc cgtgtgcgct
     4561 ggtcctcgac gggcttcgcc tgctgatgct cggcttcgac gtggacccgg cgatcgcgcg
     4621 gacgctcggc gcgcgctgga gcgcttcgtg cgcggacgac ggcgagatcg ccgagcagat
     4681 cctgcgcgga gaggaggagg tccttcagag cttcgccgac gttgtggccc tgtcgcagcg
     4741 gacggtcggg ctccccgacg aggacggcga gacgatgcgg gcgaacgggg aggagttcct
     4801 gacatacctc cgctacctcg acgccgggca tccggcgctc tccggcgcct tcctcgacgc
     4861 gctgcgccgc gccctcggcc accacggcgt ggcctcgctg gagcggtcga ccgccctcga
     4921 ggaggcgctc ctgcgcctgt accggtcgca gcaccggacc gagcacctgg tccccgccgt
     4981 cctcgccatc ctcgaccgcc ggcttgagcg gatcgagcag ctccgccagg ccgtcggccc
     5041 ggagttccag gcgctgctcg accgcctggt ggccgtgacg cgtggacgcc acttgggggt
     5101 gagcgacctg gcgcgcgagg tccgctaccg ctacttcgac cagcccctga tcgaggaggt
     5161 ccggcggtcg gcgtacgcgc gcgccgagga tgacctgcac gcgcttgcgg cgagcccgag
     5221 cggtcccgac cgcgaggcgc gcatccgggc gctcgtggag tgtccccagc cgctcaagcg
     5281 tctgctcacg gaacggatgc aggcggccgc cggcgaggcc cgggccgcga tgctcgaggc
     5341 gctcgcccgg cgctactacc gcatccgcga ggtcgaggac gcacggaccc ggctggtcga
     5401 cggccggcac gtgttcacgg cccgttaccc acacgacggt gcgacgatcc acctcgtcgc
     5461 cacccacggc gcctggccgg agctggcccg agcgtggtcg gtggcgatcg gggaggccac
     5521 cgccgcgccg gcaggcgacg aggtcgtgat cgacctctac gtctggcgcg gcggcccgcc
     5581 cgaggcggcg gagacaacgt ccgagcggat cgccgccgtc ctcgacgcgg caccgatcga
     5641 ccgcccgatc aggcgggtcg tggtggcgct atcgggtccg ggcgcgtccc tcgaggctgc
     5701 ggcgatggag cacttcacgt tccgcccggg cccggagggg taccgggagg agcggcccta
     5761 ccgcggcctg catccgatga tggggaagcg gctcgacctg tggcggctgt cgcggttcac
     5821 gatcgaccgg ctgccctccg tggaggacgt ctatctcttc cacggcgtgg cgcgggagaa
     5881 cccgaaggac gagcggctgt tcgcgctcgc cgaggtgcgc gacctgaccg tggcgcgcga
     5941 cggcgacggc cgcttggtcg gcgtgccgca gctcgagctg atgctcaccg aggcgctgga
     6001 ggcgatccgc ctgtaccagt cgcaccgccc gccgggcaag cgcctgcagt ggaaccgcgt
     6061 cctgctgaac gcgtggccgc cggtggacct gccgatcggg gagctgtacg ggatcgtgca
     6121 ctcgctggcg ccgctggccg agggcctcgg cctgcagaag gtggtggtcc gggcccgcgt
     6181 gctcgatgag gtgaccggtg aggtgcacga ccgcgtcctg cacatctcga acccggccgg
     6241 gcgcgggctg gccctgcgct tcgacgagcc gtccgacgtg ccgatcccgc ccttgtcgcc
     6301 ctaccggcag aaggtggtcc agacgcgcca gcgcgggctg acctacccgt acgagatcgt
     6361 ccggatgctc acgccgcctc ccggcacgcc ggccgacttc ccgcccgggg agttcctgga
     6421 gcacgacctg ggggaggacg ggagcctcgt cgccgtggac cgttcccacg gggagaacgc
     6481 ggcgaacgtg gtggtcgggg tgatcacgaa ccacaccgac gcgcacccgg acggcatgcg
     6541 ccgcgtggcg atcctcggcg acccgagccg cagtctgggg tcgctcgccg agcccgagtg
     6601 ccgccggatc atcgccgcgc tcgacctggc cgagcggatg ggggtgccgc tcgagtggtt
     6661 cgcggtgtcg gccggggcga agatcgcgat ggacagcggc accgagaaca tggactggat
     6721 cggccgcgtc ctgcgccggc tgatcgagtt cacgcagggc ggcggcgagg tcaacgtgct
     6781 cgtctgcggg atcaacgtcg gtgcccagcc ctactggaac gccgaggcca cgatgctcat
     6841 gcacacgaag ggcatcctgg tgatgacgcc ggagagcgcg atggtgctca ccgggaagca
     6901 ggcactcgac tactcgggcg gggtctcggc cgaggacaac gtcgggatcg gcggctatga
     6961 ccgggtgatg ggtccgaacg gccaggcgca gtattgggcg gccgacctcg acgaggcctg
     7021 ccggatcctg ttccggcact acgagcacac ctacgtccag cccggcgagc ggttcccgcg
     7081 ccgcgtgccg accaccgacc cgatcgaccg cgacgtccgt gccttcccgc accgtgcgag
     7141 cggcgagggc ggcttcgcga cgatcggcga cgtgttctcc gagcgcgcca acccggagcg
     7201 gaagaagccg ttcgacatcc gcagcgtcat gcgcgcggtg gtcgaccgcg accacgcggt
     7261 gctggagcgg tggggcggga tgcgcgacgc ggagatcgcc gtggtctggg acgcgcacct
     7321 cggcggcatc ccggtctgcc tgctggggat cgagtcgcga ccgctgcccc ggcacgaggt
     7381 ggccccggcc gacggcccgg actactggac ctcgggcacg ctgttcccgc tgtcgtcgaa
     7441 gaaggtcgcc agagcggtca acgcggcgag cggcgtccgc ccgctggtgg tgctggcgaa
     7501 tctctccggc ttcgacggct cgccggagtc gctccggaat ctgcagctcg agtacggcgc
     7561 ggagatcggc cgggccgtcg tgaacttcga cggaccgatc gtcttctgcg tggtctcccg
     7621 ctaccacggc ggcgcgttcg tggtcttctc gcgcacgctg aacgagcaca tcgagatcgc
     7681 cgcggtggag ggttcgttcg cctcggtgat cggcggggcg ccggcggcgg ccgtggtgtt
     7741 cgcccgcgag gtcgacgagc ggaccaagtc cgacccgcgg gtcgcgtcac tcgcgcagga
     7801 gctggcggag gtcgcgcccg gcgagcgcgg acgcctcctc gccgcgctgg agcgggcgac
     7861 cgcgtccgtg cgctcggaga agctcggcga ggtggccgcg gaattcgacc acatccacac
     7921 gatctatcgc gcgctggagg tcggatcggt ggaccggatc atcgccccgt ccgcgctgcg
     7981 cccgtacctg atcgacgccg tcgagcgcgg gatcgcccgc gtcctcggcc gggccgaggg
     8041 ggggacggag cgcccgagcg agctggtcga gtcccggcca tgacggttgg ggccaccgtc
     8101 cgggtcctgc tccagagggc cgacgaggta ccggtggacg acggctggct cgcgccggag
     8161 gagcgggcca tcatggacgg cctgcgcgtc ccgaagcggc gggcggactg gcgactgggc
     8221 cgctggaccg cgaagcgcgc cgtcgccgag accctgggca cggccgctgg gccgcccgcc
     8281 gacctccgca tcctggccgg gcccgacggc gccccggagg cgttcgtcgg gtggcgcccg
     8341 ctgccggtgg cgatctcgat cagccaccgg ggaggactgg ccgcctgcgc ggtcgccccg
     8401 gcgaacgcgg cgctgggctg cgacctcgag gtcgtcgagg cccggtcggc cggtttcgtc
     8461 gcggatttct tcaccgagga cgaacgggcg ctcgtcgaag cggcgccgcc ggcacggcgt
     8521 cacctgctgg cggcgctcgt ctggtcggcc aaggagagcg cgctgaaggc gctgcgcacc
     8581 ggcctgcgct cggacacccg ctccgtggag gtgtcggtcg agcccgggac cgccggggcg
     8641 tggtcgccgc tcgccgtgcg ggaccgatcc gccgaccgga ggctccgggg gtggtggcgg
     8701 gcggttgacg aggccgtgct cacggtggtg gccgatcccc ccgtgggaag gccgagcgcg
     8761 atcgcgtagc ctcgccctgc cgatggccca gacgacgacg ctccggcgaa gctacgggca
     8821 ctaccgcgag ctgttccgcg ggcggccgat gccgtt
//
LOCUS       DASPWO010000481         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_409300, whole genome shotgun sequence.
ACCESSION   DASPWO010000481 DASPWO010000000
VERSION     DASPWO010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409300"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VEO00_10960"
     CDS             complement(<1..573)
                     /locus_tag="VEO00_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008741665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYU58555.1"
                     /translation="MLELAILGLLKERSMHGYQLKKRVAETLGAFWQVSYGSLYPALK
                     RLQRSGAVEMVFPTEDVGRRKNVYRITEAGEALFQELLQETGRETGEDGKFQVRLAFF
                     RYLKPETRIGMLERRRAFLEGRLSELRRGFKEYRERIDSYTLALMRHGVEFTEQDIRW
                     LDDLIAAERRGARVKRSPRTARRAAAARIPQ"
     gene            809..1741
                     /locus_tag="VEO00_10965"
     CDS             809..1741
                     /locus_tag="VEO00_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HYU58556.1"
                     /translation="MARLRPGEPVPLRAPRRVPGGGGSPFLLALTVAAFMVAVAPAES
                     ARAAAAGSGAAAGPPRAARAETVASAFPDVVPEPVSLPRQRAVAREPDRRWALVIGID
                     EYPGASRDLLLSAADARDTARALVRRLGFPRRQVRVLLNGEATAAAIRRSARWLASRT
                     GEGGTSVLFYAGHAVQLRHLDGDREDVDEAILAADGRLVPDGVLAGALEGSAGRMWLV
                     VAACHGAGFRDALAPGRVLTAASAENDLAYESDALGNSFLVEYVVGAGLLANRSRTAE
                     AAFRTGLAAMPGAYARHRPLQLDRVAGPLSLVTP"
     gene            1770..2474
                     /locus_tag="VEO00_10970"
     CDS             1770..2474
                     /locus_tag="VEO00_10970"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HYU58557.1"
                     /translation="MSPRGESPLYPKQVLTPGRDDGRLSRAAGSADLVLDTWRRRPWP
                     RRLLKALAIVLLLAGATLIAYPFATNIYANYRQGSLAREFRSESTERRYVTRTVRTGQ
                     ALTRLRIPELGVDTIVVEGTTLSALRAGAGHYEATALPCEGGNAAIAGHRTTYSKPFA
                     RLDQLRTGDRIEIETPLGECTYRVTGAPFVVEPDDWRVIAATGGATLTLTTCHPPGSA
                     AQRLVVRAGLIGEPLR"
     gene            2525..>2801
                     /locus_tag="VEO00_10975"
     CDS             2525..>2801
                     /locus_tag="VEO00_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU58558.1"
                     /translation="MATTPQGDGALLVLQGVRKAFGSNVVLDGIDLTVGAGEAVVIAG
                     PSGSGKSTLLRCVNGLESVDGGDIRFAGRSIPNAGREIYRLRSEIGMV"
BASE COUNT          333 a         1043 c         1023 g          402 t
ORIGIN      
        1 ctggggaatg cgggcggctg cggcgcggcg cgccgtgcgg ggcgagcgct tcacccgcgc
       61 cccacggcgc tccgcggcga tcaggtcgtc gagccagcgg atgtcctgct cggtgaactc
      121 gacgccgtgg cgcatcagcg ccagcgtgta gctgtcgatc cgctcgcggt actccttgaa
      181 cccgcggcgc agctcggaca gccggccctc gagaaaggcc cggcgccgct ccagcatgcc
      241 gatccgggtc tcgggcttca ggtagcggaa gaacgccagg cgcacctgga actttccgtc
      301 ctcgccggtc tcgcggcccg tctcctggag cagctcctgg aagagcgcct cgccggcttc
      361 cgtgatgcgg tacacgttct tgcgccggcc cacgtcctcc gtgggaaaca ccatctccac
      421 ggcccccgac cgctgcaggc gcttcagcgc cgggtacagg gatccgtagg agacctgcca
      481 gaacgctccc agcgtctcgg ccacccgctt cttcagctgg tagccgtgca tcgaacgttc
      541 cttcagcagt ccgaggatcg cgagctcgag catggactcc cttccgtacg tatcgatcct
      601 atatatcgaa ccgatatatt gaaccgatac cccgcgagtg tcaacccgtg cgcgcgggtg
      661 gggccggtgg cctccgggag ccggccgtcc ctcgcgctcc ccacgctatc ggctcgtcac
      721 cggagcctcc cgtcggtccg ccgcgccact gcggaattgg ggagggcgcc cgatggtctc
      781 gcggcggtga ctggtccgat atgagggcgt ggcacggctg cgtcccggcg agcccgtccc
      841 gctccgcgca ccccggaggg taccgggggg cggcggctcg cccttcctcc tcgccctcac
      901 cgtggctgcg ttcatggtgg ccgtcgctcc cgccgagtcc gcgcgggcgg ctgccgcggg
      961 gagcggggcg gcggcgggcc cgccacgggc cgcccgcgcg gagaccgtcg cgtcggcgtt
     1021 ccccgacgtc gtgcccgagc cggtctcgct tccccgccag cgcgcggtgg ctcgcgagcc
     1081 cgaccggcgc tgggcgctgg tgatcgggat cgacgagtat ccgggcgcct cccgggacct
     1141 gctgctctcc gccgccgacg cccgggacac ggcccgggcg ctggtgaggc ggctggggtt
     1201 cccccgccgc caggtgcgcg tgctgctgaa cggcgaggcg acggcggcgg cgatccgccg
     1261 ctcggcccgg tggctggcgt cgcggacggg agaggggggc acgagcgtct tgttctacgc
     1321 tggccacgcc gtccagctgc ggcacctcga cggcgaccgt gaggacgtcg acgaggccat
     1381 cctcgccgcc gacggccggc tggtgccgga cggcgtcctg gccggcgccc tggagggatc
     1441 ggccggccgg atgtggttgg tggtcgccgc gtgccacggc gccggctttc gcgacgcgct
     1501 ggccccgggc cgcgtgctca ccgccgcgtc ggccgagaac gatctggcat acgagagcga
     1561 cgcgctcggg aactcgttcc tggtcgagta cgtcgtcggc gccgggctgc tcgcgaaccg
     1621 gtcgagaacc gccgaggcgg cgttccgcac gggcctcgcg gcgatgcccg gagcttacgc
     1681 ccgccaccgt ccgctgcagc tcgatcgcgt ggccggcccg ctctccctgg tgactccgta
     1741 agcgccgccc gctgaggcat cattgcgccg tgagcccccg cggggagtcg cccctctacc
     1801 cgaagcaggt cctgacgccc ggccgggacg atgggcggct gtcgcgagcg gccgggtcgg
     1861 ccgacctcgt gctcgacacg tggcggcggc ggccgtggcc gcggcggctg ctgaaggccc
     1921 tggcgatcgt cctcctgctg gcgggggcca ccctgatcgc ctacccgttc gccacgaaca
     1981 tctacgcgaa ctaccggcag gggagcctgg cgcgcgagtt ccgctcggag agcaccgagc
     2041 gccgctacgt cacgcggacc gtccgcaccg ggcaggcgct cacccggctg cgcatcccgg
     2101 agctcggcgt ggacacgatc gtcgtggagg ggacgaccct ctccgccctg cgggcgggcg
     2161 ccgggcacta cgaggcgacg gcgctcccgt gcgagggagg gaacgccgcg atcgccggcc
     2221 atcgcaccac gtactcgaag ccgttcgcgc gcctggacca gctgcggacg ggcgaccgga
     2281 tcgagatcga gaccccgctc ggggagtgca cctaccgcgt caccggcgcg ccgttcgtgg
     2341 tcgagcccga cgactggcgg gtgatcgccg ccaccggggg cgcgaccctc accctcacca
     2401 cctgccaccc gccggggagc gccgcccagc gcctcgtggt ccgcgcgggc ctgatcggag
     2461 agccgctccg ctagcggttc gcggcgtctc tcccggtccg tcatggtccc gtacacttcg
     2521 ggcgatggcg acgacgccgc agggcgacgg cgcgctgctg gtcctgcagg gggtccggaa
     2581 ggccttcggc tcgaacgtcg tgctcgacgg gatcgacctg acggtgggcg ccggcgaggc
     2641 cgtcgtgatc gccggcccgt cggggtcggg caagtccacc ctcctgcggt gcgtcaacgg
     2701 cctggagtcc gtggacgggg gcgacatccg cttcgccggc cggtcgatcc cgaacgccgg
     2761 ccgggagatc tatcgcctgc gctccgagat cgggatggtc t
//
LOCUS       DASPWO010000482         5256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_747914, whole genome shotgun sequence.
ACCESSION   DASPWO010000482 DASPWO010000000
VERSION     DASPWO010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747914"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VEO00_10980"
     CDS             <1..337
                     /locus_tag="VEO00_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58559.1"
                     /translation="FDPAIRIAHIGLPSMRAVLAHQRAYGARVAELARDPQTPWPTEK
                     VRARTLSVLYPVAKWGRLVARAARSSPAALGWSLLLAPWIVSAHVAWRRGYREALAAA
                     ASPVGDAAP"
     gene            382..1134
                     /locus_tag="VEO00_10985"
     CDS             382..1134
                     /locus_tag="VEO00_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58560.1"
                     /translation="MGVLRALTVAELRVTQDLTVVGILKWMLEPLSYMAVYYLLIGVV
                     LGATSRTPAYPLFLLCGLVPFRYFSGVVGESMNLITRFGAAITNRAFPRWVLPLVLVA
                     AEVPTLLLSMLLFAPMMAAFGIGPSAALLWIPVIVVVLALVSTGPAYLMAVFGLYFPD
                     FRGAAQNLIRMTYFVSTGLFPASEAKGDVLPLLPKLNPLSGLFDSLRAVVLHGNPPKP
                     FDLLYPAAVGLVLLAIGGSVYRWRQHQFAKEL"
     gene            1136..1894
                     /locus_tag="VEO00_10990"
     CDS             1136..1894
                     /locus_tag="VEO00_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU58561.1"
                     /translation="MSGRHAVSLRDVGVRFYVPRRSRLGRTPRLFRARRWHHWGLRHA
                     TVDVEHGEVVGLVGPNGSGKTTMLRVVSGIFRPDEGSIRVAGRVAPILSLTGGLENAL
                     SGWENVALSGILLGLSHAETREVGPAVAEFAGIGDEFMDAPVRVYSSGMRARLGFAIA
                     AFSRPDILALDEVLSVGDEEFKERSRQKILEMMRADHTVLIASHDVEEIVAFCDRLVR
                     IEQGEIVEIGPAEGVAERYLADHGRAGRSAHGRR"
     gene            1891..2739
                     /locus_tag="VEO00_10995"
     CDS             1891..2739
                     /locus_tag="VEO00_10995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYU58562.1"
                     /translation="MSARPAGDPTPLVSVVVAAFDEAAHMGTCLRSLRGQTYRPFEVI
                     VIDDGSRDGTAAVAARFPEVTLVRRPHGGAGSARNLGASRARGEILAFLDADMAFPSP
                     FLERLVAPMLEEGAIGTFTKDIRVANGGRRWARAHMLGRNLPPDTHFPPGFPDRWENF
                     RAVWRRDFERVGGFDDVGHGEDVTLGRKLGALAVAAPGATCWHFEPETLADVARSARW
                     AGRGVRIRETERPLSRYGPHRSIPRAARMAWRHHMPSLFLYRLVWDAGVAYGWLTRGR
                     SRPGAK"
     gene            complement(2705..3679)
                     /locus_tag="VEO00_11000"
     CDS             complement(2705..3679)
                     /locus_tag="VEO00_11000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYU58563.1"
                     /translation="MPTAPPISVIVCSYNSRQRIDRSLRSLRRQDLAEPSEVIVVDSG
                     RDGAHEYVRAAYPEVTVVRSERRLFPGPARNAGVRAAHGEVVAFLPDDGVAAPDWLRR
                     RLERHRQGYLAVGGAIANGTPSSAIGTAGYLIEYAALVPSAPVLWQQALPHCLSYHRS
                     LFDRFGLFPEGAETGEDTLFNRRLVAAGVPIALDPEIVLAHVNPTGMRAYLRHQFEHG
                     RGMAQCAVLYELEGPVGPSGQSPARAAWRLLVRYPFRRWMAAGRRLRIGRGAWLRRYL
                     TLTPLVWAGIWSAGFGAWREVLVLRRSAARGPLAVDAVTSRPGANGRA"
     gene            3651..4571
                     /locus_tag="VEO00_11005"
     CDS             3651..4571
                     /locus_tag="VEO00_11005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYU58564.1"
                     /translation="MTEIGGAVGTATMVYTAIMGDSTASAEAEAGPRPGSPSVSVVIA
                     AHDEERYLGDCLRSLAAQTYRPLEVIVVDDGSRDATAAVAARFPTVRLLRQAHLGAGS
                     ARNLGASRAHGDILVFVDGDMVLAPRFVDRLIAPMRGGGAVGTFTKDILMANPGRRWA
                     RAHVLGRNLDPQDPMAGVPDRWEIFRAVWRRDFERVGGFDDVGHGEDVTLGRKLGALA
                     EAAPGATCWHHFPDTLPDVFRSARWYGRGIRIAEDPSWRARYGPLKSTRRALRMAWRH
                     RWPPLLLYRLVWEAGAWWGWRTRERWPGAR"
     gene            4553..>5256
                     /locus_tag="VEO00_11010"
     CDS             4553..>5256
                     /locus_tag="VEO00_11010"
                     /inference="COORDINATES: protein motif:HMM:NF015527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HYU58565.1"
                     /translation="MAGGPMTDLARVVFVHASKTGGSTLRAVLRRLYRDAYRELGRDI
                     APALAELDAMPEARRERLRAVGGHAPYGVDRHLPGRWTTITLVRHPAERILSHYSYVL
                     RSPESPLHERVSAAGLGLRDYVTTFELASVINNGQTRLLGGDVDEPFRPTDGEHLERA
                     LARLRNGDVLAGTTERFDTSLLLMARSLGWSAPWYVPAKVAPQRIQRDEIDGETLRTI
                     LETNALDVELHRAAAER"
BASE COUNT          654 a         1914 c         1944 g          744 t
ORIGIN      
        1 gttcgacccc gccatccgga tcgcgcacat cggcctgccc tcgatgcggg ccgtgctggc
       61 ccaccagcgg gcgtacggcg ccagggtcgc cgagctggcc cgggacccgc agacgccatg
      121 gccgaccgag aaggtgcgag cgcgcacgct gtccgtcctc tatccggtgg cgaaatgggg
      181 ccgcctggtc gcccgcgcgg cgcgctcctc gcccgccgcg ctcggctggt cgctcctgct
      241 cgccccgtgg atcgtgtccg cccacgtggc ctggcgccgc ggctaccgcg aggcgctggc
      301 cgccgccgcg tctcccgttg gggacgccgc gccgtgacct ccgccgtacg tgtcgcggcg
      361 gcgcgcccgc aggcccgggc gatgggggtc ctgcgcgcgc tgaccgtggc cgagctgcgg
      421 gtgacccagg acctgaccgt cgtcgggatc ctgaagtgga tgctcgagcc gctctcctac
      481 atggccgtct actacctgct gatcggcgtg gtcctcggcg ccacgagccg gacccccgcg
      541 tacccgttgt tcctgctgtg tgggctcgtc ccgttccggt acttctcggg ggtcgtcggg
      601 gagtcgatga acctcatcac acgattcggt gcggcgatca ccaaccgcgc gttcccacgg
      661 tgggtcctgc cgctcgtgct ggtcgcggcc gaggtcccga cgctcctcct gtcgatgctg
      721 ctcttcgccc cgatgatggc cgcgttcggg atcggtccct ccgcggcgct cttgtggatc
      781 ccggtgatcg tcgtggtcct ggcgctcgtc tcgaccggac cggcctacct gatggccgtg
      841 ttcggcctgt acttccccga cttccgcggt gccgcccaga acctcatccg gatgacgtac
      901 ttcgtctcca ccgggctctt cccggcgagc gaggcgaagg gcgacgtcct cccgctgctg
      961 ccgaagctga atcccctgtc cggcctcttc gactcgctcc gtgccgtggt gctccacggc
     1021 aatccgccga agccgttcga tctcctgtat ccggcggccg tcgggctggt gcttctggcc
     1081 atcggcggat ccgtgtaccg ctggcggcag caccagttcg cgaaggagct gtgagatgag
     1141 cggacgccac gcggtgtcgc tgcgcgacgt cggcgtccgc ttctacgtcc cgcggcgtag
     1201 ccggctgggc cgaaccccgc gcctgttccg cgcccggcgc tggcaccact gggggctgcg
     1261 ccacgccacc gtggacgtgg agcacggcga ggtggtgggg ctcgtcggcc cgaacggctc
     1321 gggcaagacc acgatgctcc gcgtggtctc cgggatcttt cggccggacg aggggtccat
     1381 tcgggtggca ggccgggtgg cgccgatcct gtccctgacc ggcggcctcg agaacgcgct
     1441 gtcgggctgg gagaacgtcg cgctgtcggg gatcctcctc ggactgtccc acgccgagac
     1501 gcgcgaggtc gggccggccg tcgcggagtt cgccgggatc ggagacgagt tcatggacgc
     1561 gcccgtgcgc gtgtactcgt cgggcatgcg ggcacgcctg gggttcgcca tcgccgcgtt
     1621 cagccggccg gacatcctgg ccctggacga ggtgctctcc gtcggcgacg aggagttcaa
     1681 ggaacgcagc cggcagaaga tcctggagat gatgcgcgcc gaccacacgg tcctgatcgc
     1741 cagccacgac gtcgaggaga tcgtcgcgtt ctgcgaccgc ctggtgcgca tcgagcaggg
     1801 cgagatcgtc gagatcgggc cggccgaggg ggtggcggag cggtacctgg ccgaccacgg
     1861 ccgggccgga cggtccgcgc acgggcggcg gtgagcgccc ggccggccgg cgacccgacc
     1921 ccgctcgtct cggtggtcgt cgcggcgttc gacgaggccg cccacatggg gacgtgcctg
     1981 cggtcgctgc gcggccagac ctaccgtccg ttcgaggtga tcgtgatcga cgacggttcg
     2041 cgcgacggca cggccgccgt ggcggcccgc ttcccggagg tcaccctggt gcgccggccg
     2101 cacggcggcg ccgggtcggc gcgcaacctc ggcgcgagcc gcgctcgcgg cgagatcctc
     2161 gccttcctcg acgccgacat ggcgttcccg tccccgttcc tcgaacggct cgtggccccg
     2221 atgctcgagg agggggcgat cggcacgttc accaaggaca tccgcgtggc gaacggtggc
     2281 cggaggtggg cgcgggcgca catgcttggc cgcaacctgc cgccggacac ccacttcccg
     2341 cccgggttcc ccgaccgctg ggagaacttc cgcgcggtgt ggcgccgcga cttcgagcgg
     2401 gtcggcggct tcgacgatgt cggccacggc gaggacgtca cgctcgggcg aaagctgggc
     2461 gcgctcgccg tcgcggcccc gggggcgacc tgctggcact tcgagccgga gaccctggcc
     2521 gacgtggcac gctcggcccg gtgggccgga cgcggcgtgc ggatccgcga gacggagcgg
     2581 cccctgtccc ggtacggccc gcaccgctcg atcccccgtg cggcgcgaat ggcatggcgc
     2641 caccacatgc cgtcgctgtt cctctaccgg ctggtctggg acgccggcgt ggcctacggc
     2701 tggctcacgc gcggccgttc gcgcccgggc gcgaagtgac ggcatccacg gcgagcggcc
     2761 cgcgcgcggc gctccggcgc aggacgagga cctcgcgcca ggcgccgaac cccgccgacc
     2821 agatcccggc ccagacgagc ggggtcagcg tcaggtaccg ccgaagccac gccccgcggc
     2881 cgatccggag ccgccgcccg gcggccatcc agcggcggaa cgggtaccgc accagcaggc
     2941 gccaggcggc gcgagcggga gactggccgg aggggccgac cggtccctcc aactcgtaga
     3001 ggacggcgca ctgcgccatc ccccgtccgt gctcgaactg gtgccgcagg tacgcgcgca
     3061 tccccgtcgg gttcacgtgc gccaggacga tctccggatc gagcgcgatc gggacgcccg
     3121 ccgctaccag gcggcggttg aacagcgtgt cctcgccggt ctcggccccc tccgggaaga
     3181 ggccgaaccg atcgaacagc gaccgatggt acgagaggca gtgcgggagg gcctgctgcc
     3241 atagcacggg cgccgacggc acgagcgcgg cgtattcgat caggtagccg gcggtgccga
     3301 tcgcgcttga gggggtgccg ttggcgatcg cgccgccgac cgcgaggtag ccctggcggt
     3361 ggcgttcgag gcggcggcga agccagtcgg gggcggcgac gccgtcgtcg ggcaggaacg
     3421 ccaccacctc gccatgggcg gcccggaccc cggcgttgcg ggccggtccc gggaacaggc
     3481 ggcgctcgga ccgcaccacc gtgacctccg gataggctgc ccggacgtac tcgtgtgccc
     3541 cgtcccttcc ggagtccacg acgatgacct cggagggctc cgccaggtcc tgccggcgca
     3601 gcgatcgcag cgatcggtcg atccgctgcc gtgagttgta ggagcagacg atgaccgaga
     3661 tcggcggggc ggtcggcacg gcgacgatgg tatacaccgc gatcatgggc gactcgaccg
     3721 catcggccga agcggaggcc ggcccgagac cgggatcgcc ctcggtctcg gtcgtgatcg
     3781 ccgcgcacga cgaggagcgc tacctgggcg actgcctccg gtcgctcgcc gcgcagacgt
     3841 accggccgct ggaggtgatc gtggtcgacg acggctcccg cgacgcgacg gccgcggtgg
     3901 cggcgcggtt ccccaccgtg aggctgctcc ggcaggcgca cctcggcgcg gggtcggccc
     3961 gcaacctcgg cgccagccgc gcgcacggcg acatcctggt gttcgtggac ggcgacatgg
     4021 tccttgcgcc gcggttcgtg gatcggctga tcgcgccgat gcgcggcggc ggcgcggtcg
     4081 ggacgttcac caaggacatc ctgatggcga atccgggccg gcgctgggca cgggctcacg
     4141 tgctcggacg caacctggac ccgcaggacc cgatggccgg cgtcccagat cgctgggaga
     4201 tcttccgcgc ggtctggcgc cgcgacttcg agcgcgtcgg cgggttcgac gacgtcggcc
     4261 acggggagga cgtcacgctc ggccgcaagc tcggcgcgct ggccgaggcc gcccccggcg
     4321 ccacctgctg gcaccatttc cccgacaccc tcccggacgt cttccgctcg gcgcgctggt
     4381 acggccgggg catccggatc gcggaggacc cgtcgtggag ggcgcgctac ggcccgctga
     4441 agtcgacgcg acgggccctg cgcatggcct ggcgccaccg ctggccgccg ctcctcctct
     4501 accggctggt ctgggaggcc ggcgcctggt ggggctggcg gacccgggag cggtggccgg
     4561 gggcccgatg accgacctcg cccgcgtggt cttcgtccac gcgtcgaaga cgggcggcag
     4621 cacgctgcgg gccgtgctgc gccggctgta ccgggacgcg taccgggagc tggggcgcga
     4681 catcgccccg gcgctcgccg agctcgacgc catgccggag gcgcggcgcg agcggttgcg
     4741 ggccgtgggc gggcacgcgc cgtacggtgt ggaccgccac ctgccgggac ggtggacgac
     4801 gatcaccctc gtccgccacc cggccgaacg gatcctctcc cactacagct acgtcctgcg
     4861 cagccccgag tcgcccctgc acgaacgcgt gtccgcggcg ggcctcggtc tgcgcgatta
     4921 cgtcacgacc tttgagctcg cgtccgtgat caacaacgga cagacccggc tgctcggcgg
     4981 tgacgtggac gagccgtttc gtcccacgga cggcgagcat ctcgagcggg ccctggcgcg
     5041 gctgcggaac ggcgacgtcc tggcggggac caccgagcgg ttcgacacga gcctcctgct
     5101 gatggccagg tcgctcggct ggagcgcgcc gtggtacgtg ccggccaagg tggcgccgca
     5161 gcggatccaa cgggacgaga tcgacgggga gaccctgcga acgatcctcg agaccaacgc
     5221 cctcgacgtc gagctccatc gcgcggcggc cgaacg
//
LOCUS       DASPWO010000483         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_747950, whole genome shotgun sequence.
ACCESSION   DASPWO010000483 DASPWO010000000
VERSION     DASPWO010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747950"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VEO00_11015"
     CDS             <1..643
                     /locus_tag="VEO00_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58566.1"
                     /translation="FDPDLVRAFFNISLGRLTWTAGVAAWLAQLPFVGTVPRAGAAVG
                     RATAPVPAFGSTAAGAGAVAFGVVLAIGAPAGGPADINSLEGAPGGWGGRPPVSGSGT
                     VKVAAATIKAPGVNAAEAAGTADGGGDPSGPGEQAATPPGPPAQVPVGSPEDRSSDPP
                     PVDPPATPPPDAAGGGGGGGLAIGTDGCPPGQLVQDWLDHPLGGPPGLDGRPC"
     gene            1103..2359
                     /locus_tag="VEO00_11020"
     CDS             1103..2359
                     /locus_tag="VEO00_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYU58567.1"
                     /translation="MRVAVRSRNLRSLRSWAEDAAKGGADTDDSRHATDVLTLATALS
                     AHDRRTRGHSERVRSLTMLVAAEMGIEGEESERLQWAALLHDIGKLTVPPEILNKKGA
                     PDNREWKILQRHPGNGAEIASPLGDWLGDSISAVGQHHERWDGNGYPEGLAEDKIGLA
                     ARIVAVTDSFETMTAVRSYKKPQKPAAARAELARCSGTHFDPGVVRSFMKISIGRLTL
                     TVGAASWFAQMPFVGLLPRAGASVTELVSAAPAISGTVAATAAVALGLSVAATPAPAE
                     AGGGAPQGTPAAAHQPVEDHTPTWGGGWGVRDELSAPPEGTPVASPPPDVPGSPDAAT
                     PDPGTPTGTPTTTPGSGGGTDPSPGPPSDPGNGNGSPPAGAGPPADPGNGNGSPPVDP
                     GAGHGPPADPGNGNGNSGQGQGTVTP"
BASE COUNT          334 a          938 c          907 g          287 t
ORIGIN      
        1 cttcgacccc gacctggtgc gtgcgttctt caacatctcg ctcggccgcc tcacctggac
       61 cgcgggtgtg gcggcgtggc tcgcacagct gccgttcgtg ggcacggtgc cgcgcgccgg
      121 cgccgcggtc ggtcgggcga ccgcgccggt gcccgcgttt ggaagcacgg cggcaggcgc
      181 cggcgcggtc gcgttcggcg tggtgctggc gatcggcgcg ccggcgggcg ggccggccga
      241 catcaattcc ctggaaggcg cccccggtgg ctggggaggc cgacccccgg tgtccgggtc
      301 cggcaccgtc aaggtggccg ccgccacgat caaggcaccg ggcgtcaacg cagccgaggc
      361 cgcgggcaca gccgacggcg gtggcgaccc gtcggggccg ggcgagcagg ccgcgacgcc
      421 tccgggcccg cccgcccagg tcccggtcgg ttcccctgag gaccgctcga gcgacccacc
      481 tccggtggac cccccggcga ctccgcctcc cgacgcggcg ggtgggggcg gaggaggcgg
      541 cctcgccatc gggaccgacg gctgcccgcc gggccagctg gtccaggact ggttggatca
      601 ccccctggga ggtccgcccg ggctcgacgg gcgcccctgc tagccgcgct acagcgcggc
      661 cgcccggccg ccgatatcgc gacgtgggaa cggcgggggg acgaaacggg atccttcgga
      721 cgaagcggtc cgctggcctt gacgcgcgct ctccaaccgt catagtgaca ctctgcgtga
      781 gtttttaggg gcggagcgtg agtgaagcac ggaacgtgaa caaagacgac cgcaatcggt
      841 gggaagagcg cccgatcctg agcgcgctcc tgcggttggc ggtgctcgga gttccgatcg
      901 gcgcatcggt ggtggccggg atcgcggtgg gccgcgtcct gcccccggcg cacggatgga
      961 cgggcgtggt cggccaactg gcggtcgtgc tggccagctc gacgatcgtc ctgctcgtcg
     1021 tggaacggat ggcacgccgc gcgctgccgc tgcggcgctg ctgcgcctct cgctcgtgtt
     1081 ccccgagaag gcgccgtcac gcatgcgggt cgcggtccgg agccgcaacc tgcgctcact
     1141 gcgctcgtgg gccgaggacg cggcgaaggg aggcgctgac accgacgact cccgtcacgc
     1201 caccgacgtg ctcacgctcg cgaccgccct cagcgcgcac gaccgccgca cgcgaggcca
     1261 ctccgagcgc gtgcgttccc tcacgatgct ggtcgccgcc gagatgggga tcgaggggga
     1321 ggagtccgag cgcctccagt gggccgcgct cctccacgac atcggcaagc tcaccgtccc
     1381 gcccgagatc ctcaacaaga agggcgcgcc cgacaaccgg gagtggaaga tcctccagcg
     1441 ccacccgggg aacggcgccg agatcgccag cccgctgggc gactggctgg gcgactcgat
     1501 cagcgccgtc ggccagcacc atgagcgctg ggacgggaac ggctatcccg aggggctggc
     1561 cgaagacaag atcgggctcg ccgcccgcat cgtcgccgtg accgactcgt tcgagacgat
     1621 gaccgcggtt cgctcctaca agaagcccca gaagcccgcg gccgcccgcg ccgagctggc
     1681 gcgctgctcc ggtacccatt tcgatcccgg cgtcgtccga tcgttcatga agatctcgat
     1741 cggccgcctg accttgaccg tgggcgcggc gtcgtggttc gcgcagatgc cgttcgtggg
     1801 actgctgccc cgggcgggcg ccagcgtcac cgagctcgtc agcgcggccc cggcgatcag
     1861 cggcacggtg gccgccaccg ccgcggtcgc gctcgggctc agcgtggcgg cgaccccggc
     1921 gcccgccgaa gcggggggtg gcgcgcccca aggcacgccg gccgccgccc accagcccgt
     1981 cgaggatcac accccgacct ggggtggcgg ctggggcgtc cgcgacgagc tcagcgcacc
     2041 gccggagggg acgccggtcg cgtcgccccc gcccgacgtt ccgggctcgc cggacgcggc
     2101 cacgcccgac cccgggactc ccaccgggac tcccaccacc acccccgggt ccggcggcgg
     2161 caccgatccc agccctggcc cgccatctga tcccgggaac ggcaacggga gcccgcccgc
     2221 cggcgccgga cctccggccg acccgggcaa cggcaacggg agcccgccgg tcgaccccgg
     2281 cgccggccac ggccccccgg cggacccggg gaacggcaac ggcaacagcg gccagggtca
     2341 gggcaccgtc accccctgac cggtcggcgc gagcgaagcg tcagcgccgc gagcggaacc
     2401 gccgcaggcg cagcgagttc gtcaccacga acacgctcga gaagcccatg gcggcggccg
     2461 cgatca
//
LOCUS       DASPWO010000484         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_999871, whole genome shotgun sequence.
ACCESSION   DASPWO010000484 DASPWO010000000
VERSION     DASPWO010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999871"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VEO00_11025"
     CDS             complement(<1..381)
                     /locus_tag="VEO00_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58568.1"
                     /translation="MRGNADSDFAVARLNGDGSLDQRFHGDGRVAVDFGVNELGQAVA
                     LQTNGKIVVGGGGPRALIIGTPPVKERSFLLARLQGCTISGTNGDDILAGTDGNDVIC
                     RLSGNDVLTGKSGNDLLIGGYGADT"
     gene            complement(378..767)
                     /locus_tag="VEO00_11030"
     CDS             complement(378..767)
                     /locus_tag="VEO00_11030"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58569.1"
                     /translation="MAVQDDGKIVIVGTWVNAAPDFVFFAARFATTMSLDPTFNAAGP
                     TPGVKDVRETAAGSAVAVRPNGRIVAGGSAVDTPDFLAAQLLPNGTPDPSFGTSGLKP
                     VDFPGAELLILTTSPCRRTGRRSSPEP"
     gene            complement(794..952)
                     /locus_tag="VEO00_11035"
     CDS             complement(794..952)
                     /locus_tag="VEO00_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58570.1"
                     /translation="MATLGGTLYGVAAAPSGKIVVAGSIDMATDRDTFVARRTTTGAA
                     NGSASIAI"
     gene            1316..1633
                     /locus_tag="VEO00_11040"
     CDS             1316..1633
                     /locus_tag="VEO00_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58571.1"
                     /translation="MRILRVLPLAAPALLILSGTALAQTTPNPTVSPEPIGGWTIWQG
                     WLAIALAVLLVVGVVAAYVRFSPQFYGSQERPKRRQLPPGARTAASLAIPRQGAGRGE
                     SRE"
     gene            1630..2688
                     /locus_tag="VEO00_11045"
     CDS             1630..2688
                     /locus_tag="VEO00_11045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HYU58572.1"
                     /translation="MTAAADRLVDWPLAARVGRRVAGAGPKLDPAERAAVTEDFSELV
                     PRAGALVSEFTNLAPGPERPRAWVMTRGEWIDQNLRGFRRVIDPFAGRVMKRDDGRLA
                     GFRRAALGAQLGLLLGYLSRRVLGQYDLFLPPEDQGLLYFVAPNVVGVERRYRFPARD
                     FRTWVALHEVSHQVQFGGVPWLRGHISGLVDDYFESMHLDPRELIDALRRAVREVRSG
                     DAEWRGIGFLVLLMTPEQRQTFRRMQAVMSLLEGHGNFVMDTLAHRERVGDVARMKRT
                     LRERRRATPGLEKAFQKAIGFEAKSRQYDVGERFVAEVVERVGMEGFNRVWEDAGNLP
                     TLEEVGRPERWVHRVGAS"
     gene            2690..>3466
                     /gene="tilS"
                     /locus_tag="VEO00_11050"
     CDS             2690..>3466
                     /gene="tilS"
                     /locus_tag="VEO00_11050"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HYU58573.1"
                     /translation="MSPSERRPPAVARVLERLVSTVRHRGLIEPGQTVLLAVSGGPDS
                     TCLLHAMARVSRLLHVRLAVAHVDHGMRTDSAADAAYVERSARAVGLPVRVLRIEGRP
                     SRGASVEAFLRGERYRLLEAARRDAGVDLVATAHTADDQAETVLMALLRGAGLSALGG
                     IAPRRDRVIRPLLDVTRDETAAFCRALRLRPRSDPMNLEPGYLRVAVRQQVLPAIARA
                     TGREVRATIGRTADALREDATLLDALAARASRRIVRRLPAS"
BASE COUNT          425 a         1278 c         1250 g          513 t
ORIGIN      
        1 ggtgtcggcc ccgtagccgc cgatcagcag gtcgttcccg gacttgccgg tcaggacgtc
       61 gttcccggag aggcggcaga tcacgtcgtt gccgtcggtg ccggcgagga tgtcgtcgcc
      121 gttcgtcccg gagatcgtgc acccctggag tctggcgagc aggaaggacc gctccttcac
      181 cgggggcgtg ccgatgatta atgcgcgggg accaccgccc cccacgacga tcttgccgtt
      241 ggtctggagg gcgacggcct ggccaagctc gttcacgccg aagtccaccg cgacccgccc
      301 gtcgccgtgg aaccgctggt ccaggctccc gtcgccgttc agacgggcga cggcgaagtc
      361 tgagtccgcg tttcccctca cggttccggc gaggaccgcc tgcccgttct cctgcagggc
      421 gaggtcgtca ggatgagcag ctccgcgccc gggaagtcca ccggcttgag gccgctcgtc
      481 ccgaaggacg ggtccggcgt gccgttcggc aggagctgcg cggcgaggaa gtcgggcgta
      541 tcgacggcgc tccccccggc gacgatcctg ccgttcggcc ggacggccac ggcgcttccc
      601 gccgcggtct cgcgcacgtc cttgaccccg ggggtggggc ctgcggcatt gaacgtcggg
      661 tcgaggctca tcgtcgtggc gaaccgggcg gcgaagaaga cgaagtccgg cgccgcgttc
      721 acccaggtcc ccacgatgac gatcttcccg tcgtcctgga ccgccacgtc ggtcgcgtcc
      781 tcggagtcgt cgctcagatc gcgatggacg cggagccatt tgcggcgccg gtggtcgttc
      841 gccgagccac gaacgtgtcg cggtccgtcg ccatatcgat cgaaccggcg acgacgatct
      901 tcccggacgg agccgcggcc acgccgtaca gcgttccgcc gagcgtggcc atggggtcga
      961 gctcggcgcc gatggcgaac cccggaccga ccccgccgcg ttgaacgtgg tgtcgagcga
     1021 tccgttcggg ttgaatcggc ccagcgcggg atcgtcgtcg accctccccg ccgccaggat
     1081 cttgccgttg tcctggagcg ccagggcgtt gatcgtcccg ccgtcgctga gcccgaccgg
     1141 cagcacgacc ttgccgcccg tgccgaacga cgggtcgagc gagccatcgg gcgtgtaccg
     1201 ggccatcgcc cagtcgctgc gcgacgagtc acttacgcgg ccagcgcgaa ccgcgcggcg
     1261 agcacggggg gtcgacacaa tggctccacg tgcacgcttg ctagaatgcg ccctcgtgcg
     1321 catccttcga gttctcccgc tcgccgctcc ggccctcctg atcctctccg ggacggcgct
     1381 cgcgcagacc acgcccaacc cgaccgtcag ccccgagccg atcgggggtt ggacgatctg
     1441 gcagggctgg ctggcgatcg cgctcgccgt cctgctggtc gtcggggtgg tcgccgccta
     1501 cgtgcggttc agcccgcagt tctacggctc gcaggagcgg ccgaagcggc gccagctccc
     1561 gccgggggcg cgcacggcgg cctccctcgc catcccccgc caaggtgcgg gacgggggga
     1621 gtcgcgcgag tgaccgccgc cgccgatcgc ctggtcgact ggcccctggc cgctcgcgtc
     1681 gggcggcggg tcgccggcgc cggcccgaag ctcgaccccg ccgagcgagc ggcagtgacc
     1741 gaggacttct cagagctcgt tcctcgtgcg ggagcgctcg tctcggagtt cacgaacctt
     1801 gccccggggc ccgagcggcc gcgggcgtgg gtgatgaccc gcggcgagtg gatcgaccag
     1861 aacctgcgcg gcttccgccg ggtgatcgac ccgttcgccg ggcgggtgat gaagcgtgat
     1921 gacggacggc tcgccgggtt ccgccgcgcg gcgctcgggg cgcagctcgg cctgctgctc
     1981 ggatatctct cgcgccgggt cctcggccag tacgatctct tcctcccgcc cgaggaccag
     2041 ggcctgctct acttcgtcgc accgaacgtc gtcggcgtcg aacggcgcta ccgcttcccc
     2101 gcccgcgact tccggacctg ggttgccctg cacgaggtct cgcaccaggt ccagttcggc
     2161 ggcgtcccgt ggctgcgcgg ccatatctcg ggcctggtgg acgactactt cgagtcaatg
     2221 cacctcgacc cgcgcgagct gatcgacgcg ctgcgccggg ccgtccgcga ggttcgttcc
     2281 ggcgacgccg agtggcgggg gatcggattc ctcgtgctgc tgatgacgcc cgagcaacga
     2341 cagacgttcc ggcggatgca ggcggtgatg tcgttgctcg aggggcatgg caacttcgtc
     2401 atggacacgc tggcccaccg cgagcgcgtc ggggacgtcg cgcggatgaa gcggaccctg
     2461 cgcgagcggc gccgcgccac ccccgggctg gagaaggcgt tccagaaagc gatcgggttc
     2521 gaggccaagt cgcgccagta cgacgtcggg gagcgcttcg tcgccgaggt ggtcgagcgc
     2581 gtcgggatgg agggtttcaa ccgcgtgtgg gaggacgccg ggaacctgcc gaccctcgag
     2641 gaggtcggcc ggcccgagcg gtgggtgcac cgggtcgggg cttcgtgaga tgtctccgag
     2701 cgagcggcgg ccgccggccg tcgcccgggt cctggagcgc ctggtctcca ccgtccgcca
     2761 ccgcggcctg atcgagcccg gccagacggt cctcctcgcc gtctccggcg gccccgactc
     2821 cacctgcctg ctgcacgcga tggcgcgggt cagccgcctg ctccacgtgc gcctggcggt
     2881 ggcgcacgtg gaccacggga tgcgcaccga ctcggcggcc gacgcggcct acgtcgagcg
     2941 ctcggcccgc gcggtcggcc ttcccgtccg cgtgctccgc atcgagggcc ggccctcgcg
     3001 cggcgcctcg gtggaggcct tcctgcgcgg cgagcgctac cgactgctcg aggcggcccg
     3061 ccgggatgcc ggcgtcgacc tggtggcgac ggcccacacg gcagacgacc aggccgagac
     3121 ggtgctgatg gccctgctgc ggggagccgg cctctccgcg ctcggcggca tcgccccccg
     3181 ccgcgatcgg gtgatccgcc cgctcctcga cgtgacgcgc gacgagaccg ccgcgttctg
     3241 ccgggccctg cggctgcgcc cccgaagcga cccgatgaac ctcgagcccg gctacctgcg
     3301 cgtcgccgtc cggcagcagg tcctcccggc gatcgcgcgc gccacggggc gggaggtgcg
     3361 ggcgacgatc ggacggaccg ccgacgccct gcgcgaggac gccacgctgc tcgacgcgct
     3421 cgccgcccgc gcctcccggc gcatcgttcg tcgcctcccg gcatcg
//
LOCUS       DASPWO010000485         4398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_662003, whole genome shotgun sequence.
ACCESSION   DASPWO010000485 DASPWO010000000
VERSION     DASPWO010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662003"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VEO00_11055"
     CDS             <1..1297
                     /locus_tag="VEO00_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58574.1"
                     /translation="IAELEAVQAALEAARTAAAKAGAAAERAGDDRATAEDALARAAE
                     ADPALPCPTCGRPLGDDFAEYVRHCRARLAEGKKAETAARTGERRAAAALAGAERAVA
                     AAAKRGDAVRQAVERRRALEQRVAELGEIVDPLLERFGGEVPDVPALAEAASREVTVG
                     KRLAELDAERRRLEDTERDLDSCRDELEAAAARLAELDGEDGRLGFDVEEHARLGKER
                     DESRALLDRARADERVAAAALADSGREAAEAEGRLAQARETAAEAEALFDEARLLERV
                     QLLLDGFRDHLVARVGPELSREAEALFRDLTGGAYEDLRIDEETMAIQIADGADWLPI
                     ERFSGSETDLANLALRVAISMHMSRVSGADVGLLVLDEVLASLDAERRDRFVQVIGGL
                     SSRFNQILVITHADQVKDQFPAVIEVRPTGRRRSQAVLV"
     gene            1307..2533
                     /locus_tag="VEO00_11060"
     CDS             1307..2533
                     /locus_tag="VEO00_11060"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU58575.1"
                     /translation="MEPRGPESLWRRVRDLPRPVWVLCAGTFVNRFGSFVSVFLVLYL
                     TASGYGPAQAGLAVAAYGAGSLVSALAGGYLADRLGRRNAIAISMFSAAGAALGLGAA
                     RGLLPIASLAALFGLASELYRPAASALLADLVPQGRRVPAYAAYRLAVNAGFAAGPAV
                     AGFLADISFWWVFVGEAATSAVYGFVALAGLPESRRVRRHEEEPGAALRSILGNRPFV
                     LLCLGTLLGAFVYMQATTTLPLHVRDSGHSAAVFGVLISLNGLLIVLAELPLTSFTHR
                     LPARPVIAAGMILSGIGFGVNAWAHSILPLAAGVLIFTVGEMTAAPIGSAYAADLADG
                     RYQGRYQGAFTLMFALGAVLAPAAGTALYARSPVLLWAACFAVAVAAGGLILLSGGSR
                     RVTRAAVVTSETDGRA"
     gene            2586..4043
                     /locus_tag="VEO00_11065"
     CDS             2586..4043
                     /locus_tag="VEO00_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYU58576.1"
                     /translation="MSFRRVVALRVLLLAALLFVATRVSSPETFAFSSGDLSIRMVPA
                     IPGGRIVLHLGPFGDLWWNTHVSPLDVHASFVLSPTIRDLPALEELGDLRAGFLARKA
                     VWVLLTGLLGGALVAIPAGARRLGRASAAGVAALVLVAGGLVGLSVVTFDADALADPH
                     YRGPIEDAPRVLRLLEEVERDWEGVSRNINQVVAGLARVHAQIVGGGPGAETPTVRFL
                     FVSDLHNNPVGLLIARELASGFDVDAVLDGGDFSDRGTAIEGELFAQFGAFDVPYVVA
                     PGNHEDRGALARVGAIPMVTILDARATDTVTFHGITVLGDRDPNSFFVGDDPNNERAE
                     RLFPVLCERLRQRWLAVRPEVVLVHNPRLGECAAAEAEEEVAPLVFAWGHLHRASYDE
                     RGSVVSISPGTSGANGIKSPVAAPYGFALLEFSIRDGLLTSACSFQFRTPGELAETAC
                     HVFPGGRVPQPQETPTETVSPTETVSPTAGATVSP"
     gene            complement(4033..>4398)
                     /locus_tag="VEO00_11070"
     CDS             complement(4033..>4398)
                     /locus_tag="VEO00_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HYU58577.1"
                     /translation="DHGIAVVEDPELMKRLADRRIPFDVCPNSNIVIANRYPRLEDHP
                     FRRMREAGLLVTLNTDDPAMTDLDLGIEYRTVAAAQGMSLEEMFGVALDGVEATWLDD
                     DEKRRLRASFEREIAALRD"
BASE COUNT          482 a         1612 c         1656 g          648 t
ORIGIN      
        1 catcgccgag ctcgaggcag tgcaggccgc gctggaggca gcgcggaccg cggcggcgaa
       61 agccggggcg gcggccgagc gggccggtga cgaccgcgcc acggccgagg acgcgctcgc
      121 ccgtgccgcc gaggccgatc cggccctccc ctgccccacc tgcggccgcc cgctcggcga
      181 cgacttcgcc gagtacgtcc gccactgcag ggcgcgcctc gccgagggga agaaggcgga
      241 gaccgcggcc cggaccggag agcggcgggc cgccgccgcg ctcgccgggg ccgagcgcgc
      301 cgtggctgcc gccgcgaaac gcggcgacgc ggtgcggcag gcggtcgagc gccgccgcgc
      361 gctggagcag cgcgtcgccg agctcgggga gatcgtcgac ccgctcctcg agcgcttcgg
      421 cggcgaagtg ccggacgtgc cggcgctcgc cgaagccgcg tcgcgcgagg tcaccgtcgg
      481 caagcgcctg gccgagctcg acgccgagcg acggcggctg gaggacacgg agcgcgacct
      541 ggattcctgc cgggacgagc tcgaggccgc cgcggcccgc ctcgccgagc tcgatggcga
      601 ggacggcagg ctggggttcg acgtcgagga gcacgcccgc ctggggaagg agcgggacga
      661 gtcccgcgcg ctcctcgatc gggcacgggc cgacgagcgg gtcgcggcgg ccgccctcgc
      721 cgactccggt cgggaggccg ccgaggccga ggggcgcctg gcgcaggcgc gggagacggc
      781 ggccgaggcc gaggcgctct tcgacgaggc ccgcctgctc gagcgcgtcc agctgttgct
      841 cgacggcttc cgggaccacc tcgtcgcgcg cgtcgggccg gagctgtccc gcgaggccga
      901 ggcgctgttc cgcgacctga ccggcggcgc gtacgaggac ctgcgcatcg acgaggagac
      961 gatggcgatc cagatcgccg acggcgccga ctggctcccg atcgagcgat tctccggctc
     1021 ggagaccgac ctggcgaacc tggcgctgcg cgtggcgatc tcgatgcaca tgtcgcgcgt
     1081 ctccggtgcc gacgtcggcc tgctggtcct cgacgaggtg ctcgcctcgc tcgacgccga
     1141 gcgccgggac cggttcgtcc aggtgatcgg cgggctgtcg tcccgcttca accagatcct
     1201 ggtgatcacg cacgccgacc aggtgaagga ccagttcccg gcggtgatcg aggtgcgccc
     1261 gaccggccgg cggcgcagcc aggcggtcct ggtctaggag gcaccgatgg agccgcgggg
     1321 cccggagagc ctgtggcggc gggtccggga cctgccgcgg ccggtgtggg tgctctgcgc
     1381 ggggacgttc gtcaaccggt tcggatcgtt cgtctccgtc ttcctggtcc tgtatctcac
     1441 ggcgagcggc tacggcccgg cgcaggccgg acttgccgtc gccgcgtacg gtgccgggag
     1501 cctggtgtcg gcgctcgccg gtgggtacct ggcggacagg ctggggcgcc ggaacgcgat
     1561 cgcgatctcg atgttctcgg ccgccggggc cgcgctcggc ctcggcgcgg cccgtggcct
     1621 gctcccgatc gcgtcgctcg ccgcgctctt cgggctcgcc tccgagctct accggccggc
     1681 cgcgtccgcg ctcctggcgg acctcgtccc gcagggccgg cgcgttcccg cctacgctgc
     1741 ctaccggctg gccgtcaacg ccgggttcgc ggccgggccg gcggtggccg ggttcctggc
     1801 ggacatctcg ttctggtggg tgttcgtcgg cgaggcggcc acctcggcgg tctacgggtt
     1861 cgtcgcgctc gctggcctcc cggagagccg ccgcgtccgc cggcacgagg aggagcccgg
     1921 cgcggcgctg cgctcgatcc tcggcaaccg tccgttcgtc ctgctgtgcc tggggacgct
     1981 gctcggcgcc ttcgtctaca tgcaggccac cacgacgctg ccactgcacg tgcgcgactc
     2041 gggccactcg gccgccgtct tcggggtcct gatctcgctg aacgggctgc tgatcgtgct
     2101 cgcggagctg ccgctgacgt cgttcacgca ccgcctgccg gcgcgcccgg tgatcgcggc
     2161 cggcatgatc ctgtccggga tcgggttcgg tgtgaacgcc tgggcgcact cgatcctgcc
     2221 gctcgccgcc ggcgtcctga tcttcacggt tggcgagatg acggcggcgc cgatcggctc
     2281 ggcctacgcc gccgacctgg ccgacgggcg gtaccagggc cgctaccagg gggcgttcac
     2341 cctgatgttc gcgctcggcg ccgtgctggc gccggcggcg gggacggcac tctacgctcg
     2401 ctcccccgtc ctactgtggg ccgcctgctt cgccgtcgcc gtcgccgcgg gcgggctgat
     2461 cctgctctcc ggcggctctc gccgcgtgac ccgggcggcc gtcgtgacgt cggagaccga
     2521 cgggcgggcc tgagcccggg ctgcccgacc gcgccccgcc agctctgatc cctatcatcg
     2581 gtgcggtgtc gttccggcgc gtcgtcgccc tgcgcgtgct gctcctcgcc gcgctcctct
     2641 tcgtggccac ccgcgtctcc tcgccggaga cgttcgcctt ctcctccggg gacctgtcga
     2701 tccggatggt cccggcgatt ccaggcggac ggatcgtgct gcacctcggt ccgttcgggg
     2761 acctctggtg gaacacgcac gtgagcccac tggacgtgca cgccagcttc gtgctgtcgc
     2821 ccacgatccg tgacctcccg gccctcgagg agctgggcga cctccgggcc ggcttcctgg
     2881 cgcgcaaggc ggtctgggtg ctgctgaccg gcctgctggg cggcgcgctg gtggccatcc
     2941 ccgccggggc gcgccggctg ggccgcgcgt cggcggccgg ggtcgcggcc ctcgtgctgg
     3001 tggcgggcgg gctcgtgggc ctgtcggtgg tgacgttcga cgcggacgcg ctcgcggacc
     3061 cgcactaccg cgggccgatc gaggacgcgc cgcgggtgct gcggctgctg gaggaggtgg
     3121 agcgcgactg ggagggcgtc tcgcgcaaca tcaaccaggt ggtcgccggg ctggctcgcg
     3181 tccacgcgca gatcgtcggc ggcgggccgg gggccgagac cccgaccgtg cggttcctgt
     3241 tcgtctcgga cctgcacaac aacccggtcg gactgctgat cgcccgcgag ctggcgtcgg
     3301 ggttcgacgt cgatgccgtg ctggacggcg gcgacttctc cgaccgcggc acggcgatcg
     3361 agggcgagct gttcgcccag ttcggggcgt tcgacgtgcc ctacgtcgtc gcccccggga
     3421 accacgagga ccgcggcgcg ctggcccgtg tgggggccat ccccatggtg acgatcctcg
     3481 acgcgcgggc gaccgatacg gtgacgttcc acgggatcac cgtcctcggc gaccgcgatc
     3541 cgaactcctt cttcgtcggc gacgacccga acaacgagcg ggccgagcgg ctgttcccgg
     3601 tgctgtgcga gcgcctgcgc cagcgatggc tcgccgtgcg ccccgaggtc gtcctggtgc
     3661 acaatccgcg gctcggcgag tgcgcggcgg cggaggccga ggaggaggtg gcgccactgg
     3721 tgttcgcctg gggacacctg caccgggcgt cgtacgatga gcgcggctcg gtggtctcca
     3781 tctcgcccgg gacgagcggg gcgaacggga tcaagtcccc cgtggccgcc ccgtacggat
     3841 tcgccctgct cgagttctcg atccgcgacg gcctgctgac gtcggcgtgc tcgttccagt
     3901 tccgcacgcc gggcgagctc gccgagacgg cctgccacgt cttccccgga ggacgggtgc
     3961 cgcagcctca ggaaacgccg accgagaccg tgtccccgac cgagaccgtg tccccgaccg
     4021 ccggggcgac cgtcagtccc tgagggcggc gatctcccgc tcgaacgacg cgcgcaggcg
     4081 gcgcttctcg tcgtcgtcca gccaggtggc ctcgaccccg tcgagcgcca caccgaacat
     4141 ctcctccagg ctcatcccct gcgccgcggc caccgtgcgg tactcgatcc cgaggtcgag
     4201 gtcggtcatc gccgggtcgt cggtgttcag ggtgacgagc aggcccgcct cgcgcatccg
     4261 ccggaacggg tggtcctcca ggcgcgggta gcggttggcg atgacgatgt tcgagttcgg
     4321 acagacgtcg aacgggatcc gtcggtccgc gaggcgcttc atcagctccg ggtcctcgac
     4381 caccgcgatg ccgtggtc
//
LOCUS       DASPWO010000486         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1170115, whole genome shotgun sequence.
ACCESSION   DASPWO010000486 DASPWO010000000
VERSION     DASPWO010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170115"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VEO00_11075"
     CDS             <1..706
                     /locus_tag="VEO00_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HYU58578.1"
                     /translation="WILNGRKHFITGGDYADFALVFAVTDSEKRARGGITAFFVDADS
                     PGYSVSRLQKTMSPYQTPVELTFENVEVPDENVLGEVGYGFYSAVKGINGARLQISAN
                     ALGVAQHLLDRSLEYANDRVAFERPIAANQFVQGHLVDSFAELEQARLLVYKLADDID
                     NGADGRREAALAKLVATEAVGRIADRAIQVHGGNGFMTELGIEAWYREVRAMRLYEGT
                     SEILRANIAKTLGLKV"
     gene            complement(643..1152)
                     /locus_tag="VEO00_11080"
     CDS             complement(643..1152)
                     /locus_tag="VEO00_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58579.1"
                     /translation="MDGPTAGGDRPDGRRPPSLPWVLTIVSIAVAVLATSGFVYLLLK
                     RTVGPGTILRDFYQAVHNGDCDASYDLLAHTVAERVEPGDWCSAIQQAGYPATFSIEQ
                     TLLVDDVAVLTVEEAGDGATLDWGLERAGRSWVVRDFPAQRDVLRAAQTFRPSVLAMF
                     ARRISDVPS"
     gene            1175..2179
                     /locus_tag="VEO00_11085"
     CDS             1175..2179
                     /locus_tag="VEO00_11085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU58580.1"
                     /translation="MPDPLDRRLACHLQPGRSLDEAIECARLADRLGYESIWAYQTVD
                     RDPLQVLARYAAVTERAVLGTAVVPIATRHPIQLAMEAATLDEASGGRLRLGIGVSHR
                     LTVEGWYGLALEDPVGWLEEYASVLRELFAHGAVGFEGRHVTARFTFVRYRARRDLPI
                     WFAAMGPRMLRLAARLADGVVLWMCSPGHVREHIRPVLDAALAEAGRSWEEFDLVAPV
                     PVALSEDLAAGRNAFRRAAYAYVQLPYYRKEIAAAGFGEDLEAFDRTLAAAGPADAMA
                     ALSDAFVDAYAAVGDAGAARAKIEEYRAAGVTLPSVVTSGRPPAAAPAEAALEALAGA
                     "
     gene            2190..3050
                     /locus_tag="VEO00_11090"
     CDS             2190..3050
                     /locus_tag="VEO00_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYU58581.1"
                     /translation="MAVGGDRFVGGRLASLLPDEAHDVVALARASSDTRRLRALGAEI
                     AVGDVTDRASVAASMEGCDGGFHVAAWYEVGVAERDRMFRTNVGGTENVLEAARATSL
                     PKLVYCSTVGALGWHRSDLPGGVYGPAIPRWSAGSCGRPGVDAGHGARRRRVQLGPRR
                     RRGPRAPADPRAGRARRRLRPPRRAGHRRPLPGPGGTPVRPSPAAGAHPRRGVLRAVA
                     PLSGPVLRLAGFGPRTLTEALGSLSGERSWAYSWDRAARELGYAPRDLETGLRQTVEA
                     LRRTRNGDRD"
BASE COUNT          389 a         1096 c         1191 g          430 t
ORIGIN      
        1 gtggatcctg aacggccgca agcacttcat caccggcggc gactacgcgg acttcgcgct
       61 ggtgttcgcg gtgaccgaca gcgagaagcg tgcccgcggc gggatcaccg cgttcttcgt
      121 cgacgcagac tcgcccggct actcggtcag ccgcctgcag aagaccatgt cgccgtacca
      181 gaccccggtg gagctgacgt tcgagaacgt cgaggtcccg gacgagaacg tgctcggcga
      241 ggtcggctac ggcttctact cggcggtgaa gggcatcaac ggcgcgcgcc tgcagatctc
      301 ggcgaacgcg ctcggcgtgg cacagcacct gctcgaccgt tcgctcgagt acgcgaacga
      361 ccgcgtggcg ttcgagcggc cgatcgcggc gaaccagttc gtgcaggggc acctggtgga
      421 ctcgttcgcg gagctggagc aggcgcgcct gctcgtctac aagctcgccg acgacatcga
      481 caacggggcc gatgggcggc gcgaggccgc gctggccaag ctcgtcgcca cggaggcggt
      541 cggccgcatc gccgaccgcg ccatccaggt gcacggcggc aacggcttca tgaccgagct
      601 cgggatcgag gcctggtatc gggaggtccg ggcgatgcgc ctctacgagg gcacgtcgga
      661 gatcctgcga gcgaacatcg ccaagacgct cggcctaaag gtctgagcgg cccgcagcac
      721 gtcgcgctgc gcggggaagt cgcggaccac ccaggagcgg ccggcgcgct ccaggcccca
      781 gtcgagggtc gcgccgtcgc ccgcttcctc gaccgtcagc accgccacgt cgtcgacgag
      841 gagcgtctgc tcgatgctga acgtcgcggg gtagcccgcc tgctggatgg ccgagcacca
      901 gtcgcccggc tcgacgcgct ccgcgaccgt gtgggcgagc aggtcgtagg aggcgtcgca
      961 gtcgccgttg tgcaccgcct ggtagaagtc tcgcaggatg gtgcccgggc ccacggtgcg
     1021 cttcagcagc aggtagacga atccgctggt cgccagcacc gccacggcga tggagacgat
     1081 cgtcaggacc caggggagcg agggcggccg gcggccgtcg gggcggtcgc cgccggcggt
     1141 cggaccatcc acgggcggta ggatagcccc tcccatgccc gacccattgg accggcggct
     1201 ggcctgccac ctccagcccg gccgttccct ggacgaggcg atcgagtgcg cccggctggc
     1261 cgaccggctc ggctacgaga gcatctgggc ctatcagacc gtggaccggg acccactgca
     1321 ggtcctggcg cggtacgccg ccgtcaccga gcgggccgtc ctgggaaccg cggtggtgcc
     1381 gatcgccacg cggcacccga tccagctggc catggaggcg gccacgctcg acgaggcgtc
     1441 cgggggccgc ctgcggctcg gcatcggggt cagccaccgc ctgaccgtcg aggggtggta
     1501 cggcctggcg ctcgaggacc cggtcgggtg gctcgaggag tacgcctcgg tcctgcgcga
     1561 gctgttcgcg cacggcgcgg tcgggttcga gggccgccac gtcacggcgc ggttcacctt
     1621 cgtccgctac cgggcgcggc gggatcttcc gatctggttc gccgcgatgg gaccgcgcat
     1681 gctgcggctg gccgcgcggc tggcagacgg ggtcgtgctc tggatgtgct cacccgggca
     1741 cgtccgtgag cacatccggc cggtgctcga tgcggcgctg gcggaggccg gccgctcctg
     1801 ggaggagttc gacctggtcg cgccggttcc ggtggcgctc tccgaggacc tcgcggccgg
     1861 aaggaacgcc ttccgccgcg cggcctacgc ctacgtccag ctgccctact accgcaagga
     1921 gatcgccgcc gccgggttcg gggaggacct ggaagcgttc gaccgcacgc tcgcggccgc
     1981 cggcccggcg gacgcgatgg cggcgctgtc ggacgcgttc gtcgacgcct acgccgcggt
     2041 cggagacgcc ggggcggccc gggcgaagat cgaggagtac cgcgccgccg gcgtgaccct
     2101 cccgtcggtg gtgacctccg ggcggcctcc ggcggccgcc ccggccgagg cagcgctcga
     2161 ggccctggcg ggtgcctgat ggcgcggctg tggctgtcgg gggtgaccgg ttcgtcggcg
     2221 ggcggctggc ctcgctgctg cccgacgagg cgcacgacgt cgtggcgctg gcccgcgcat
     2281 cgagcgacac ccggcgcctg cgggcgttgg gagcggagat cgccgtcggc gacgtcacgg
     2341 accgggcctc ggtggccgcc tccatggagg gctgcgacgg cggattccac gtcgcggcgt
     2401 ggtacgaggt gggcgtggcc gagcgggacc ggatgttccg gacgaacgtc ggcgggaccg
     2461 agaacgttct cgaggcggcg cgggccacgt cgctgccgaa gctcgtctac tgctcgaccg
     2521 tgggggcgct cggctggcac cgctcggact tgcccggcgg ggtgtacggg ccggcgatcc
     2581 ctcggtggtc ggccgggtcc tgtggtcgtc cgggggtgga tgctggccac ggcgctcgac
     2641 gacgccgagt tcagctgggt ccacgtcgac gacgtggccc gcgggcacct gctgaccctc
     2701 gagcggggcg cgcccggcgc cgactacgtc ctccgcggcg agccggccac cgtcggcccc
     2761 ttcctggccc gggcggcacg cctgtccggc catcgcccgc cgcgggtgcg catccccgcc
     2821 ggggggtcct gcgcgcggtc gcacccctct ccgggccggt gctgcgcctg gcgggcttcg
     2881 ggccgcgcac cctgacggag gcgctcggct cgctgtccgg cgagcggtcg tgggcctact
     2941 cctgggaccg cgccgcgcgc gagctggggt atgcgccgcg cgacctcgag acggggctgc
     3001 gccagacggt cgaggcgctg cgccgtacgc ggaacggaga tcgcgactag gtgacgacgc
     3061 cgaggccgtc gatcaggacc gtcctccccg acgagctggc gttctt
//
LOCUS       DASPWO010000487         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_151001, whole genome shotgun sequence.
ACCESSION   DASPWO010000487 DASPWO010000000
VERSION     DASPWO010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151001"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VEO00_11095"
     CDS             complement(<1..213)
                     /locus_tag="VEO00_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HYU58582.1"
                     /translation="MRIAKYHGTGNDFVLVEDLDDRAPLPSALIAALCDRHRGVGADG
                     LIRIVRGALFGGRADFTMDYANADGEP"
     gene            complement(210..1139)
                     /gene="miaA"
                     /locus_tag="VEO00_11100"
     CDS             complement(210..1139)
                     /gene="miaA"
                     /locus_tag="VEO00_11100"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HYU58583.1"
                     /translation="MTDATRRPLLGLVGPTASGKTRASLAIAVALDAEIVCVDSMLVY
                     RGMDVGTAKPAGAERAAVRHHLIDVAEPDERFSVARFQRLAAEALTAIAGRGRTALLV
                     GGSGLYYRAVVDGLAFPATDPAVRVALETEAVAVGAAGLHRRLAAFDPAAAAKIEPGN
                     VRRTVRALEVAAITGRTFSSFAAMWEAYPAERVRAAGIEIEPAALAARIESRARAMFE
                     GGLLEEARGLAEAGLGEALTARQAIGYQEALAHLDGQLSLEEAVARTVRRTRELARRQ
                     MAWFRRDPRIRWFRTGAEGGSAVADELREYLAA"
     gene            complement(1136..2494)
                     /gene="miaB"
                     /locus_tag="VEO00_11105"
     CDS             complement(1136..2494)
                     /gene="miaB"
                     /locus_tag="VEO00_11105"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HYU58584.1"
                     /translation="MGRFLIRTFGCQMNEHDSERIAGLLTANGMLGVDDVADADVVVL
                     NTCAIRENADRRLYGNLGHLRPLKERNPGMRIVVAGCLAQKDRGAIQRRAPWVDVVVG
                     THALPELLDLLRRAETEGPQMDVREYTETFPSALPALREVRHHAWVQVAVGCDNACTF
                     CIVPLVRGRQRSRPLGEILAEVQGLGRDGVVEVTLLGQNVNTYGRDLHVPGSAARPRF
                     GELLRAVDGVAGIRRVRFTSPHPHDFTPDVIEAMAECAAVCEHIHFPLQSGSDRVLAD
                     MRRSYRVARYLGWLERIRGAIPGIAVTTDVIVGFPGETEDDFAETLRTVEAARFDAAF
                     TFQYSPRPGTAAGTRDDQVPAEVVTERFSRLVALQEAISMERNRALEGASVEVLIEGA
                     GRKGGAQGRTRTNKVVNLPGADLEPGTFVSALVERAHPHHLTGRPVAADDLARPMAAA
                     AS"
     gene            complement(2519..>2673)
                     /locus_tag="VEO00_11110"
     CDS             complement(2519..>2673)
                     /locus_tag="VEO00_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HYU58585.1"
                     /translation="RVMVKPEDVDDLSAQVLARDIAKRVEEELQYPGQIKITVVRETR
                     AVEFAK"
BASE COUNT          357 a         1027 c          936 g          353 t
ORIGIN      
        1 gggctcgccg tcggcgttcg cgtagtccat cgtgaagtcg gcccggccac cgaacagggc
       61 gccgcggacg atccggatca gcccgtcggc cccgacgcca cggtgacggt cgcagagggc
      121 ggcgatgagc gccgagggga gcggcgcgcg gtcgtcgagg tcctcgacaa ggacgaagtc
      181 gttgccggtg ccgtggtact tcgcgatcct cacgctgcca ggtactccct cagctcgtcg
      241 gccaccgcgg acccgccctc ggcgccggtc cggaaccagc ggatccgcgg gtcccgccgg
      301 aaccaggcca tctgccggcg ggccagctca cgggtccgcc ggacggtgcg ggccacggcc
      361 tcctccagcg acagctggcc gtccaggtgc gccagtgcct cctgataccc gattgcctgg
      421 cgggcggtga gcgcctcgcc caaccccgct tctgccagcc cgcgtgcctc ctccagcagc
      481 ccgccctcga acatagcgcg ggcgcgcgat tcgatccgcg cggcgagggc cgccggctcg
      541 atctcgatcc cggcggcgcg cacgcgctcc gccggatagg cctcccacat ggcggcgaac
      601 gacgagaagg tccggccggt gatcgcggcc acctccaggg cgcgcacggt ccggcggacg
      661 tttccgggct cgatcttcgc cgctgcggca gggtcgaacg ccgccaggcg gcggtgcagg
      721 ccggcggccc cgacggccac cgcctcggtc tcgagcgcca cccggacggc cggatcggtc
      781 gccgggaatg ccagcccgtc gacgacggca cggtagtaca ggccggaccc gcccaccagg
      841 agcgccgtgc gcccgcgacc ggcgatcgcg gtcagcgcct ccgcggcaag gcgctggaag
      901 cgggccaccg agaatcgctc gtcgggctcg gcgacgtcga tcaggtggtg ccgaaccgcc
      961 gcgcgctcgg ccccggccgg cttcgccgtc ccgacgtcca tgccccggta gacgagcatc
     1021 gagtccacgc acacgatctc ggcgtcgagc gccaccgcga tcgccaacga ggcccgggtc
     1081 ttcccgctcg cggtcgggcc gaccagtccg agcagcggac gccgggtcgc atccgtcacg
     1141 aggccgccgc cgccatcggc cgcgcgaggt cgtccgcggc gaccgggcgg ccggtcaggt
     1201 ggtgcgggtg ggcccgctcg acgagcgcgc tgacgaacgt tcccggctcg aggtcggcac
     1261 ccgggaggtt caccaccttg ttcgtccgcg tccggccctg ggcgccgccc ttgcgcccgg
     1321 ccccctcgat cagcacctcg accgacgccc cctccagcgc acggttgcgc tccatcgaga
     1381 tcgcctcctg cagcgccacc aggcgggaga agcgctcggt gaccacctcg gccgggacct
     1441 ggtcgtcgcg ggtcccggcg gccgttcccg gccgcggcga gtactggaac gtgaacgcgg
     1501 cgtcgaaccg cgccgcctcg accgtccgca gggtctcggc gaagtcgtcc tcggtctccc
     1561 ccgggaagcc cacgatcacg tcggtggtca ccgcgatgcc ggggatcgcg ccccggatcc
     1621 gctccagcca cccgaggtag cgcgcgacgc ggtaggagcg acgcatgtcc gcgagcacgc
     1681 ggtccgatcc ggactgcagc gggaagtgga tgtgctcgca gaccgcggcg cactcggcca
     1741 tggcctcgat cacgtcgggg gtgaagtcgt gggggtgcgg gctggtgaac cggacgcggc
     1801 ggatccccgc gacgccgtcc accgcgcgca ggagctcgcc gaatcgcggg cgggcagccg
     1861 acccggggac gtgcaggtcg cgcccgtacg tgttcacgtt ctgcccgagg agggtcacct
     1921 cgacgacgcc gtcgcggccg agcccctgca cctccgccag gatctccccc agcggccgcg
     1981 agcgctgtcg tccccgcacg agcggcacga tgcagaacgt gcacgcgttg tcgcagccca
     2041 ccgcgacctg cacccatgcg tggtggcgga cctcgcgcag cgccggcagc gccgaaggga
     2101 acgtctcggt gtactcgcgg acgtccatct gcgggccctc ggtctccgcc cgccgcagca
     2161 ggtcgaggag ctccggcagg gcatgcgtgc cgacgacaac gtccacccac ggcgcacggc
     2221 gctggatggc cccgcggtcc ttctgcgcca ggcacccggc caccacgatg cgcatcccgg
     2281 ggttccgttc cttcagcggg cggagatgcc caaggttgcc gtacagccgc cgatcggcgt
     2341 tctcgcggat cgcgcacgtg ttcagcacca cgacgtcggc gtcggccacg tcgtccaccc
     2401 cgagcatgcc gttcgccgtg agcagcccgg cgatccgttc ggagtcgtgc tcgttcatct
     2461 ggcacccgaa ggtgcggatc aggaagcgtc ccatccggcc ttcgagtcta gccgccactc
     2521 acttcgcgaa ctcgacggcg cgggtctcgc ggacgaccgt gatcttgatc tggccggggt
     2581 actgcagctc ctcctcgacc cgcttggcga tgtcgcgcgc gagcacctgc gccgacaggt
     2641 cgtcgacgtc ctccggcttg accatgacgc gca
//
LOCUS       DASPWO010000488         4619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_410499, whole genome shotgun sequence.
ACCESSION   DASPWO010000488 DASPWO010000000
VERSION     DASPWO010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410499"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..226
                     /gene="rpmI"
                     /locus_tag="VEO00_11115"
     CDS             38..226
                     /gene="rpmI"
                     /locus_tag="VEO00_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HYU58586.1"
                     /translation="MKQKTHRGAAKRFRISGTGKVLRRKATGNHMLTKKSGSQKRRIT
                     GTAVVEAEAKTVRRMLGS"
     gene            228..608
                     /gene="rplT"
                     /locus_tag="VEO00_11120"
     CDS             228..608
                     /gene="rplT"
                     /locus_tag="VEO00_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018024748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HYU58587.1"
                     /translation="MARVKRSVHGRKKRREVLAAAKGYRGARSRRYRVAKEQLLHSGV
                     YQYRDRRDRKGQFRRLWIARIGAAARTHGMSYSTFIAGLAAAGIEVDRKILAELAVHD
                     PAAFSSLVQAARAARPGVDAAAAS"
     gene            669..2192
                     /locus_tag="VEO00_11125"
     CDS             669..2192
                     /locus_tag="VEO00_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HYU58588.1"
                     /translation="MRPDDVPRANPPRETDGAVTSRPRLSSPRNRQVVRAARLKKRAF
                     RERDRRFLVEGPQAVGAALGTAPVDALFVAEGAHGELATRADRLGVPVYSATPDVIGH
                     LSSTVTPQGVVAAAGFVDMPLADLPARPDLVALLCAIRDPGNTGTIIRSAEAAGADAV
                     VVTEGSVDVYNAKAVRSTAGSLFHLPLVREVSAADAVTALRARGMQVIAADGRAETPV
                     EAVDLSRPTAILFGNEAWGLPPEVRDLADLVVRVPIRGRAESLNVAAAAAVILFVAAD
                     AASRTAPPPAASAGPAGTEAEFTALVAAAAHDLRSPLAAMQSVVGMLLERWARLTDEQ
                     RDELLGALAGETERAQVAIRRVVDGARLSMGSLPLDPVPTDLRELAERAVALAAGQRS
                     GLRIELGGGPARALVDRDRMLPAAMALLEVAAWAESDAPIEVRTIAGEPAALEVRRAG
                     AALTPADLTRLFTPQGGDRALGLWEARALAEAHGGALDAEAGDDGLVLRLRLPGPSA"
     gene            2231..3250
                     /locus_tag="VEO00_11130"
     CDS             2231..3250
                     /locus_tag="VEO00_11130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009646181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HYU58589.1"
                     /translation="MEEEVARGRQLAAAAGSLAELERAEVALLGRKAPLAGVQRSLGG
                     LSEDDRRRVGQATNEARETIRALLEERREALAAAEAAARLEADRADMTLPGRRPRLGS
                     LHPLTIVEDRIVDAFTRMGYRVAEGPEIEDDWHNFEALNIPPDHPARTMKDSLYVDVP
                     GMLLRTETSAVQIRTMESQPPPVFVVAPGRTYRREAVDATHLSVFHQVEGLAVDEGIS
                     FAHLKGTLEAFARELFGERVRVRLNPDYFPFVEPGCQVAVSCFVCDGVGCRVCGNGWL
                     ELLGAGMVHPKVLENCGYDSERYTGFAFGMGIERVAMVSFGVRDMRLFVEGDVRFLEQ
                     FEGAA"
     gene            3247..>4619
                     /gene="pheT"
                     /locus_tag="VEO00_11135"
     CDS             3247..>4619
                     /gene="pheT"
                     /locus_tag="VEO00_11135"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HYU58590.1"
                     /translation="MRIVLSWLRELCPTDLSAGELAELLTARGAEVESVERPWAGLAG
                     VVVARVLEVADHPGSDTLCLARVDAGDTGRYEVVVGVRNMGPGDLVPFAPPGARVPVL
                     PEPLGAREIRGVLSNGMLCSPAELAISPDHGGILVLDEDLAPGRDLAEALGLDDAVLD
                     LEVTPNRPDFLSVLGIAREVAAATGTPLVPPDTSVAEDEEAAAGVATVEVLDAERCPR
                     YLARIVRGVRHVPAPVRAQARLTAAGMRPISAVVDATNYAMLEIGQPLHPFDLALLKG
                     PGIVVRRAQPEERLVTLDRVERAFTADDLMICDAERPVAVAGVMGGRVAEVSEGTEDI
                     LLESAAFERGGVQRTRRRIDLSTEASVRFERGVDPEAPPVGADRACRMLAEWCGARIL
                     RGVVEVGGAPPRRRIAMRATRASLVIGYPVSVADAEEVFDRLGMTHEREDKDRLAVEV
                     PGYRVDL"
BASE COUNT          583 a         1665 c         1768 g          603 t
ORIGIN      
        1 cggcggagat ggagcccgaa aggacgagca cgagcggatg aagcagaaga cgcaccgtgg
       61 ggccgcgaag cgcttccgga tctcggggac cggcaaggtc ctccgaagga aggcgaccgg
      121 caaccacatg ctgacgaaga agagtggcag ccagaagcgg cggatcaccg ggaccgccgt
      181 cgtcgaggcg gaggccaaga ccgtccgccg catgctcggg agctgacatg gcccgggtga
      241 agcggagcgt ccacgggcgc aagaagcggc gcgaggtcct ggcggcggcg aagggctatc
      301 gcggcgcgcg gtcccggcgc taccgcgtgg cgaaggagca gctgctccac tccggcgtct
      361 accagtaccg tgatcgccgc gatcgcaagg gccagttccg gcgtctgtgg atcgcccgga
      421 tcggcgcggc cgcccgcacg cacgggatga gttactcgac gttcatcgcc gggctcgcgg
      481 cggccggcat cgaggtcgac cgcaagatcc tggccgagct ggccgtacac gatcccgccg
      541 ccttctccag cctggtccag gcggcgcgtg ccgcccgccc gggcgtggac gcggcggccg
      601 cctcctagcg ggccgatgcc cgccggggcg accggggggg tttgggcggg caggtcgccc
      661 tggagagagt gagaccggac gacgtcccgc gagcgaaccc cccaagggag acggacgggg
      721 ccgtcacgag ccgcccgcgc ctctcgagtc ctcgcaaccg gcaggtcgtc cgcgcggcgc
      781 gcctgaagaa gcgcgccttc cgcgagcgcg accgccgatt cctggtcgag ggtccacagg
      841 ccgtcggcgc cgccctggga accgccccgg tcgacgcgct cttcgtcgcc gagggcgcgc
      901 acggcgagct cgccacgcgc gccgaccggc tcggcgtccc cgtctactcg gcgaccccgg
      961 atgtgatcgg ccacctttcg tcgaccgtca caccacaggg tgtcgtggcc gcggcgggtt
     1021 tcgtcgacat gcccctggcc gatctgccgg cgcggccgga cctcgtcgcg ctgctctgcg
     1081 cgatccgcga tcccgggaac accgggacca tcatccgctc ggcggaggcg gccggtgccg
     1141 acgccgtcgt ggtcaccgag gggtccgtcg atgtctacaa cgccaaggcg gtccgttcga
     1201 ccgccggctc gctgttccac ctcccgctcg tccgcgaggt ctccgccgcc gacgcggtga
     1261 cggccctgcg cgctcgcggc atgcaggtga tcgccgccga cggccgcgcc gagacgccgg
     1321 tggaggccgt cgacctctca cgcccaacgg ccatcctgtt cgggaacgag gcctggggcc
     1381 tgccgcccga ggtgcgggac ctggcggacc tggtggtcag ggtcccgatc cggggtcggg
     1441 ccgagtcgct gaacgtcgcc gccgcggctg ccgtgatcct gttcgtcgcc gccgacgcgg
     1501 cctcgagaac agcgccgccg cccgctgcct cggccggccc cgcgggcacg gaggcggagt
     1561 tcacggcgct cgtggcggcc gccgcgcacg acctccgctc gccgctcgcg gcgatgcagt
     1621 cggtggtcgg gatgctcctg gagcgatggg cgcggctcac cgacgagcag cgcgacgaat
     1681 tgctgggcgc cctcgccggc gagacggagc gggcgcaggt cgcgatccgg cgcgtggtgg
     1741 acggcgcccg gctgtcgatg ggatcgctgc cgctcgatcc cgtccccacg gacctgcgcg
     1801 agctggccga gcgcgcggtg gcgctcgcgg ccgggcaacg gtcgggcctg cggatcgagc
     1861 tgggcggcgg cccggccagg gccctggtcg accgggaccg gatgctcccc gccgcgatgg
     1921 ccctcctcga ggtcgccgcc tgggccgaga gcgacgcgcc gatcgaggtg cgcaccatcg
     1981 ccggggagcc cgccgcgctc gaggtgcgcc gggcgggcgc tgcgctgacg cccgccgacc
     2041 tgaccaggct attcaccccg cagggcgggg accgcgcgct ggggctctgg gaggcgcgcg
     2101 ccctggccga ggcccacggc ggggcgctcg acgccgaggc cggagacgac ggcctcgtgc
     2161 tgcggctccg gctgcctggc ccctccgcct agactcgcat cccgtggagc cgaccgacgc
     2221 cctgcgcgtg ctggaagagg aggtcgccag gggacggcag ctcgccgccg cggcgggaag
     2281 cctcgccgag ctcgaacgtg ccgaggtcgc gctactcggg cgcaaggcgc cgctggccgg
     2341 cgtccagcgt tccctgggcg gcctctccga ggacgaccgc cgccgcgtcg gacaggccac
     2401 gaacgaggcg cgggagacga tccgcgcgct gctcgaggag cgccgcgagg ccctcgctgc
     2461 ggccgaggcc gccgcgcgcc tggaggccga ccgggccgac atgacgctgc ccggccggcg
     2521 cccccggctc ggatcgctcc atccgctgac gatcgtcgag gaccgcatcg tcgacgcgtt
     2581 cacgcggatg ggctaccggg tggccgaggg ccccgagatc gaggacgact ggcacaactt
     2641 cgaggcgctg aacatcccgc ccgaccaccc ggcgcggacg atgaaggact cgctctacgt
     2701 cgacgtaccc gggatgctgc tgcggacgga gacttcagcg gtgcagatcc ggacgatgga
     2761 atcgcagcca ccgccggtgt tcgtcgtcgc gccgggccgg acgtaccggc gggaggcggt
     2821 cgacgccacg cacctgtcgg tcttccacca ggtggagggc ctcgcggtcg acgagggaat
     2881 ctcgttcgcg cacctgaagg ggaccctcga ggcattcgcg cgggagctgt tcggtgagcg
     2941 cgttcgcgtg cgcctcaacc ccgactactt cccgttcgtc gagcccgggt gccaggtggc
     3001 cgtctcgtgc ttcgtctgcg acggcgtcgg ctgccgcgtg tgcggcaacg gatggctgga
     3061 gctgctcggc gcgggcatgg tgcacccgaa ggtcctggag aactgcggct acgactccga
     3121 gcgctacacg gggttcgctt tcgggatggg catcgagcgc gtggcgatgg tcagcttcgg
     3181 cgtccgcgac atgcgcctgt tcgtcgaggg cgacgtccgc ttcctcgagc agttcgaggg
     3241 ggcggcatga ggatcgtcct ctcgtggctt cgcgagctgt gcccgacgga cctctcggcc
     3301 ggggagctcg ccgagctgct gacggcgcgt ggagccgagg tcgagtcggt cgagcgaccc
     3361 tgggccgggc tggcgggcgt cgtcgtggcg cgcgtgctgg aggtggccga ccaccctggg
     3421 tcggacacgc tctgcctggc acgggtcgac gccggggaca ccggccgcta cgaggtggtc
     3481 gtcggcgtgc gcaacatggg acccggggac ctggttccgt tcgccccacc cggcgcgcgc
     3541 gtccccgtcc tcccggagcc gctcggggcg cgcgagatcc gcggcgtgct gtcgaacggg
     3601 atgctctgct ccccggcgga gctggccatc tcgccggacc acggcggcat cctggtcctc
     3661 gacgaggacc tggcgccggg tcgcgacctg gcggaggcgc tcggcctcga cgacgccgtc
     3721 ctcgatctcg aggtcacccc caaccgccct gacttcctct cggtcctcgg catcgcccgc
     3781 gaggtcgcgg cggcgacggg gaccccgctc gtcccgccgg acacctcggt cgccgaggac
     3841 gaggaggcgg cggcaggcgt ggccacggtc gaggtgctcg acgctgagcg ctgcccgcgc
     3901 tacctggcgc gcatcgtccg cggcgtgcgc cacgtgccgg cgccggtccg cgcgcaggca
     3961 cggctgacgg cggcggggat gcgcccgatc tcggcagtcg tcgacgccac gaactacgcg
     4021 atgctcgaga tcggccagcc gttgcacccc ttcgatctcg cgctgctgaa gggaccgggg
     4081 atcgtcgtcc gccgcgccca gcccgaggag cggctcgtca ccctcgaccg ggtcgagcga
     4141 gccttcacgg cggacgacct gatgatctgc gacgcggaac ggccggtggc cgttgccggc
     4201 gtgatgggcg gccgggtggc ggaggtatcc gagggcaccg aggacatcct gctcgagagc
     4261 gccgcgttcg agcgcggcgg ggtccagcgc acccggcggc ggatcgacct gtcgaccgag
     4321 gcgtcggtac ggttcgagcg cggcgttgac cccgaggcac cgccggtcgg ggccgaccgg
     4381 gcgtgccgca tgctggccga gtggtgcggc gctcgcatcc tgcgcggggt cgtcgaggtc
     4441 ggcggcgcgc ccccgcggcg gcggatcgcg atgcgcgcga cccgcgcgtc gctcgtgatc
     4501 ggctacccgg tgtcggtcgc cgacgccgag gaggtcttcg accggctggg gatgacccac
     4561 gagcgtgagg acaaggaccg gctggcggtc gaggtccccg ggtaccgcgt ggacctgga
//
LOCUS       DASPWO010000489         6255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_749319, whole genome shotgun sequence.
ACCESSION   DASPWO010000489 DASPWO010000000
VERSION     DASPWO010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749319"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2011)
                     /gene="recG"
                     /locus_tag="VEO00_11140"
     CDS             complement(<1..2011)
                     /gene="recG"
                     /locus_tag="VEO00_11140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HYU58591.1"
                     /translation="MSPAKALSLDAAVREVGGVASMPTGPGRRGGSPVHELLARRLGV
                     ETVDDLLHHYPRRYLDRSKTVPIRDLKINEEATVIGRVEKTSRVFTRRRQAMVTVIIR
                     DEHRGVLDLFWFNQAWPFRRFRAGMEVMAFGKLGAFRGRLRMQNPTVDVLRGDDGESI
                     HVGRIIPIHPATEGVSPGMIRRLVHDALGRVALADPVPPDLRPADGALGRGEALRAIH
                     FPEDEVQLSRARQRLKFDEMFTLELGVAFRKHRVERETKGVAHDLSGTLAKRFLAALP
                     FEPTEGQRAAIAEIEGDMVRPRPMNRLLQGDVGSGKTVVALYAAMVAIQSGHQAAIMA
                     PTEVLAGQHLLKVREFLAPLGAYEVGEAAPGGQLALGDGRPEPELAYALLTAAVTGKE
                     RQRVLDGVASAGIDLVIGTHALVQEGVAFHDLSLAVIDEQHRFGVHQRMVLKEKGAHP
                     DVLIMTATPIPRTLALTYYGDLDVSILSELPAGRQPIETRLARTDAERQRSYDLVREE
                     VAAGRQAFVVCAAIDESNRVEVRSAEAEADRLATEVFPDLRVALLHGRMRPAEKQARM
                     AAFRASETDVLISTTVIEVGVDVPNATVMLVENAERFGLAQLHQLRGRIGRGAHRSYC
                     VLFDESAEENEEARARLAAMVRTTDGFDLADEDLRLRGEGTLFDVR"
     gene            complement(2008..3615)
                     /locus_tag="VEO00_11145"
     CDS             complement(2008..3615)
                     /locus_tag="VEO00_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HYU58592.1"
                     /translation="MVEAVDARTLGAVMRLFGEALRAHREELNSLNVFPVPDGDTGTN
                     MLLTQEAVTHALEGTDGDLGAMGEAIARASLMGARGNSGVILSQVLRGLSDRLCREEG
                     AGGTALAEALTEASVEATRAVARPQPGTVLTVLDEAAAAARAAATGGGDLPTVAAAAL
                     SAGEEALARTPELLPELKRAGVVDAGGRGLLLMFDALLAVVSGEPLSVPVGPLGPVGH
                     TPLDAALEPASLAFSHEVMYLLEADDAAVPPLQARLAALGDSLVVVGGGGLYNVHVHV
                     NDPEAAVDAGRGAGTLREVRITSLERDVAEHCLSGQARGVRVDERVSLVAVALGGGLA
                     ELFRSLGARVVEGGPGSNPSVGELVAAIDAAPADSVLVLPNHPNAIPAAERAAAESAR
                     EVHVLMTRSIPQGIAAAAAHNPMADPADAHAALIEAAAACRWGELTRAVRDAETEAGP
                     VRSGQWLALAEGGVQGIGDDPAPLAAALVAALGADRELLTVVTGADAVPSEAAAVEAA
                     VRAAAPGLDVEVVRGGQPNYPYLIGIE"
     gene            3653..3844
                     /gene="rpmB"
                     /locus_tag="VEO00_11150"
     CDS             3653..3844
                     /gene="rpmB"
                     /locus_tag="VEO00_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HYU58593.1"
                     /translation="MAAVCDICGKTPGFGMNVSHSHRRTKRRWDPNVQRVRALIDGSP
                     RRVNVCTKCLKAGKVLRAV"
     gene            3905..5155
                     /locus_tag="VEO00_11155"
     CDS             3905..5155
                     /locus_tag="VEO00_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HYU58594.1"
                     /translation="MPDPRARGPKAPSPDRLEETRLLLRLAREVTGTLDLQEVLDRSL
                     AALRTLIDFGGGAIQLIEEGALAAAATDPPASPEAKRVRIPVGEGVSGGIAATGEPVY
                     IPDITIDPRVPAARRRAGVSSGVRSYFGAPLIVHGQPIGVLQIDAPRVDAFPPDVQAL
                     VLSFVPTITAAVQNARLYAKERDAAERLREAQQPKRDFVSMVSHELRNPLATVAGFAE
                     TLSRHLDRLDPETARDAARRIRAGVTRIERLVDDLLDLSQVERGRLALSLGPTDVGAV
                     VRTVAAEQADVTPVEVEVQEDLPAVVADEDRLHQVVSNLIVNARRYSPSGSAVRVDAR
                     SLEGWVLVSVEDHGKGIPADMLDRVFEPFFRVDDGERSGGLGLGLHLSRALIQAMGGD
                     VGVESEVGRGSRFTVRLPARAGAR"
     gene            complement(5152..5679)
                     /locus_tag="VEO00_11160"
     CDS             complement(5152..5679)
                     /locus_tag="VEO00_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016823393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58595.1"
                     /translation="MEESEEIRGWFAGRLPGEWFTEAPEVRVDREEILVIGRLAEPEV
                     GEGGSAEAAREGRIRQFREDTRQARMKIAREAEHRFGRKVSWGARCGDTGLLFTHLSV
                     PVMTRLRQPERQVLDTLVDAGVARSRSGALAWCVELVSKKQEEWLKDLREALEQVRAV
                     REQGPGDRVEGTQVV"
     gene            complement(5753..>6255)
                     /locus_tag="VEO00_11165"
     CDS             complement(5753..>6255)
                     /locus_tag="VEO00_11165"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HYU58596.1"
                     /translation="NLVVDPVFVSKHGHSLLAEDAVDALRERILPLATVVTPNLHEAG
                     GLAGFRVVDLADMERAGRAILELGARSVLMKGGHLERDASDLFLTAEGDVEWLEGSRV
                     DTRHTHGTGCILSAAIAAHLAHGRPLREAVRRGKAFVTEAIRHALALGQGIGPADPAW
                     SLRDRP"
BASE COUNT          822 a         2393 c         2217 g          823 t
ORIGIN      
        1 ggcgaacgtc gaacagggtg ccctcgcccc gcagccggag gtcctcgtcg gccaggtcga
       61 agccgtccgt ggtccggacc atcgccgcca gacgggcccg ggcctcctcg ttctcctccg
      121 cgctctcgtc gaacagcacg cagtacgagc ggtgcgcccc gcgaccgatc cgtccgcgga
      181 gctggtgcag ctgggccagg ccgaatcgct ccgcgttctc gacgagcatc acggtcgcgt
      241 tcggcacgtc gacgcccact tcgatcacgg tggtcgagat cagcacgtcg gtctcgctgg
      301 cgcggaacgc cgccatccgc gcctgcttct ctgccgggcg catccggccg tgcagcagcg
      361 ccacccgaag gtccgggaac acctcggtcg ccagccgatc ggcctcggcc tcggccgatc
      421 tcacctcgac ccggttcgac tcgtcgatcg ccgcgcagac cacgaacgcc tgccgccccg
      481 ccgccacctc ctcgcgtacc agatcgtagg agcgctgccg ctcggcgtcg gtccgggcca
      541 ggcgcgtctc gatcggctgg cggccggcgg ggagctcgga gaggatcgag acgtcgaggt
      601 caccgtaata cgtcagcgcg agcgtccgcg ggatcggggt ggcggtcatg atcagcacgt
      661 ccgggtgcgc tcccttctcc ttgagcacca tccgctggtg cacgccgaag cggtgctgct
      721 cgtcgatcac cgccagcgaa aggtcgtgga acgccacgcc ctcctgcacc agcgcgtgcg
      781 tgccgatcac gaggtcgatc cccgcggagg ccacgccgtc caggacgcgc tggcgctcct
      841 tcccggtgac cgccgcggtc aggagggcgt aggccagctc gggctcgggg cggccgtcgc
      901 cgagcgccag ctgcccgccg ggcgcggcct caccgacctc gtacgcgccg aggggcgcca
      961 ggaactcgcg caccttcagc aggtgctgcc cggcgagcac ttcggtcggg gccatgatcg
     1021 cggcctggtg gccggactgg atcgccacca tcgctgcgta cagcgcgacc acggtcttcc
     1081 cggacccgac gtcaccctgc agcagccggt tcatcgggcg cggccggacc atgtcccctt
     1141 cgatctccgc gatcgccgcg cgctgcccct cggtcggctc gaacggcagc gcggccagga
     1201 agcgcttcgc gagggttccc gagagatcgt gggccacgcc cttcgtctcg cgctccacgc
     1261 gatgcttgcg gaaggcgacc ccgagctcga gcgtgaacat ctcgtcgaac ttcaagcgct
     1321 ggcgcgcccg cgacagctgc acctcgtcct cggggaagtg gatcgcgcgg agcgcctcgc
     1381 cgcgccccag ggcgccgtcg gcggggcgca ggtccggcgg gaccgggtcg gcgagcgcca
     1441 cccgccccag ggcatcgtgg accaggcgcc ggatcatgcc cggcgacacg ccctcggtgg
     1501 ccgggtggat cgggatgatg cggccgacgt ggatgctctc gccgtcgtcg ccgcggagca
     1561 cgtcgacggt cgggttctgc atccggagac ggccgcggaa cgcgccgagc ttcccgaagg
     1621 ccatcacctc catgcccgcg cggaagcgcc ggaagggcca ggcctggttg aaccagaaga
     1681 ggtcgaggac cccgcgatgc tcgtcgcgga tgatcacggt gaccatcgcc tgacgccgcc
     1741 gcgtgaacac gcggctcgtc ttctcgaccc tcccgatgac cgtggcctcc tcgttgatct
     1801 tcagatcgcg gatcgggacg gtcttcgagc ggtccaggta gcggcgcgga tagtggtgca
     1861 gcagatcgtc taccgtctcg acgccgagcc ggcgggcgag gagctcgtgg accggcgagc
     1921 ctccccggcg gccaggcccg gtgggcatcg aggccacacc gccgacctct cgaaccgcgg
     1981 cgtcgagcga caatgccttc gcggggctca ctcgatcccg atcaggtacg ggtagttcgg
     2041 ctggccgccg cggacgacct cgacgtccag gcccggcgcg gcggcgcgga cggccgcctc
     2101 caccgcggcc gcctccgagg ggaccgcgtc cgcgccggtg acgaccgtca gcagctcgcg
     2161 gtccgcgccg agggccgcga cgagcgccgc ggccagcgga gccgggtcgt cgccgatccc
     2221 ctgaacgccc ccctcggcga gcgccagcca ctggccgctc cggaccgggc cggcctcggt
     2281 ctcggcgtcg cggacggcgc gggtgagctc cccccagcgg cacgccgccg ccgcctcgat
     2341 cagggcggcg tgggcgtcgg ccggatctgc catcgggttg tgcgccgccg ccgcggcgat
     2401 cccctgcggg atcgagcgcg tcatcaggac gtggacctcc cgggcgctct cggcggcggc
     2461 ccgttccgcg gcggggatcg cgttcgggtg gttcggaagc accagcaccg agtctgccgg
     2521 tgccgcgtcg atggcggcga ccagctcgcc gaccgacggg ttgctgcccg ggccaccctc
     2581 gaccacccgc gcgccgaggg agcggaagag ctcggcgagc ccgccgccga gggccaccgc
     2641 caccagggag acccgctcgt ccacccgcac gccccgggcc tggccggaga ggcagtgctc
     2701 ggcgacgtcg cgctcgagcg aggtgatccg gacctcgcgc agggtaccgg cccctcgccc
     2761 ggcgtcgacg gcggcctcgg ggtcgttcac gtggacgtgc acgttgtaga gcccgccgcc
     2821 gccgaccacc accagggagt ccccgagcgc ggccagccgg gcctggagcg gcgggactgc
     2881 cgcgtcatcc gcctcgagca ggtacatcac ctcgtggctg aacgccaggg acgccggctc
     2941 gagggcggcg tcgaggggcg tgtggccgac cggcccgagc ggtcccaccg gcaccgagag
     3001 cggctccccc gacaccaccg cgagcagcgc gtcgaacatc agcagaaggc ctcggccgcc
     3061 ggcatcgacc accccggccc gcttcagctc ggggagcagc tccggcgtgc gcgccagcgc
     3121 ctcctccccg gcggagagcg ccgcggcggc caccgtcggc aggtcgcccc cgccggtggc
     3181 ggcggcccgc gcggcggccg cagcctcgtc caggaccgtc agcacggtcc ccggctgggg
     3241 cctggccacg gcacgggtcg cctcgacgct cgcctcggtc agcgcctcgg ccagcgcggt
     3301 cccaccggcg ccctcctccc ggcacaggcg gtcggagagc ccacggagga cctgcgacag
     3361 gatcacgccg gagttcccgc gcgcgcccat gagggatgcc cgggcgatcg cctcgcccat
     3421 cgcgccgagg tcgccgtccg tcccctcgag cgcgtgcgtg acggcctcct gcgtcagcag
     3481 catgttcgtg ccggtgtcgc cgtccggaac cgggaagacg ttcagggagt tgagctcctc
     3541 ccggtgcgcc cggagggcct cgccgaacag ccgcatcacc gcgccgagcg tccgcgcgtc
     3601 gaccgcttcg accatcgtcg caggatagcc cgcctgctac catcgttcgg ccatggccgc
     3661 cgtctgcgac atctgcggga agacgcccgg gttcgggatg aacgtctccc actcccatcg
     3721 ccgcaccaag cgacggtggg atccgaacgt gcagcgtgtg cgggccctga tcgacgggtc
     3781 accgcgccgc gtgaacgtct gcacgaagtg cttgaaggcc ggcaaggtcc tgcgggcggt
     3841 ctgaccgcgc cacgaatcgt tggtctgttc cgacgccccc ggacgtggca gactgagggg
     3901 gtcgatgccc gacccgcgcg cccgcggtcc gaaggccccg tctcccgacc gcctcgagga
     3961 gacgcgcctg ctgctgcggc tggcccgcga ggtcaccggg accctcgatc tccaggaggt
     4021 cctggaccgc agcctggcgg cgctgcgcac cctgatcgac ttcggcggcg gggcgatcca
     4081 gctcatcgag gaaggagcgc tcgcggcggc ggccaccgac ccgccggcga gcccggaggc
     4141 caaacgggtg cggatcccgg tcggtgaggg cgtctccggc ggcatcgccg cgaccggcga
     4201 gccggtctac atccccgaca tcaccatcga cccccgcgtc ccagccgccc ggcgcagagc
     4261 gggcgtcagc agcggcgtgc gcagctactt cggcgcgccg ctgatcgtgc acgggcaacc
     4321 gatcggcgtc ctgcagatcg acgcgccgcg cgtggacgcg ttcccgccgg acgtccaggc
     4381 gctcgtgctg tcgttcgtcc cgacgatcac cgccgccgtc cagaacgcga ggctctacgc
     4441 gaaggagcgc gacgccgccg agcgtctgcg ggaggcgcag cagccgaagc gcgacttcgt
     4501 ctcgatggtc tcgcacgagc tgcgcaatcc gctggcgacg gtggccgggt tcgccgagac
     4561 gctctcccgg cacctcgacc ggctcgaccc ggagaccgcg cgcgatgccg cccggcgaat
     4621 ccgcgccggc gtcacacgga tcgagcggct ggtggacgac ctgctcgacc tgtcccaggt
     4681 tgagcgcggg cggctggccc tttcgctcgg cccgaccgac gtcggcgccg tggtgcgcac
     4741 tgtcgccgcg gagcaggcgg acgtgacgcc ggtcgaggtc gaggtgcagg aggacctgcc
     4801 ggcggtggtc gccgacgagg accggctgca ccaggtggtg tcgaacctga tcgtcaatgc
     4861 ccgcaggtac tcgccgagcg ggagtgcggt gcgcgtcgac gcccggtcgc tggagggctg
     4921 ggtgctggtg tcggtcgaag atcacgggaa gggcatcccg gccgacatgc tcgaccgggt
     4981 cttcgagccg ttcttccgcg tggacgacgg cgagcgctcc ggcggcctcg gcctcgggct
     5041 gcacctctcc cgagcgctga tccaggcgat gggcggcgac gtcggcgtcg agagcgaggt
     5101 cggacgcggg agccgcttca cggtgcggct gccggctcgc gccggggccc gctagacgac
     5161 ctgcgttccc tccacgcggt cgcccggacc ctgttcgcgg accgcccgga cctgctccag
     5221 cgcctcgcgg aggtccttca gccactcctc ctgcttcttg gacacgagct cgacgcacca
     5281 cgcgagggct ccgctgcggc tgcgggccac cccggcgtcg accagcgtgt cgagcacctg
     5341 gcgctccggc tggcgcaacc gggtcatcac cggcaccgag aggtgggtga acaggagccc
     5401 ggtgtcgccg caccgcgcac cccaggacac cttgcgcccg aagcggtgct ccgcctcacg
     5461 ggcgatcttc atccgggcct gccgggtgtc ctcccggaac tgccggatcc ttccctcgcg
     5521 ggccgcctcg gccgacccgc cctcgccgac ctcgggctcg gcgagccgcc cgatcaccag
     5581 gatctcctcg cggtcgaccc gcacctcggg cgcctccgtg aaccactccc cgggcagccg
     5641 cccggcgaac caaccccgga tctcctccga ctcctccacg ggatccctcc ttcgctcggc
     5701 ccgcggaagt gagattacat cattactctc attacgcgct gcgcacggtg gatcagggcc
     5761 ggtcgcgcag ggaccaggcg gggtcggccg ggccgatccc ctggccgagg gcgagcgcgt
     5821 ggcggatggc ctccgtgacg aacgccttcc cccgccggac ggcctcgcgc agcggccggc
     5881 cgtgcgccag gtgggccgcg atggccgcgg agaggatgca ccccgtgccg tgggtgtgcc
     5941 gcgtgtcgac gcgcgagccc tcgagccact cgacgtcgcc ctcggccgtc aggaacaggt
     6001 cggaggcgtc gcgctcgagg tgcccaccct tcatcagcac cgaccgggcc ccgagctcca
     6061 ggatcgcccg cccggcgcgc tccatgtcag ccagatccac cacccggaac ccggccagtc
     6121 cgccggcctc gtgcaggttc ggggtgacca cggtcgccag cgggaggatc cgttcgcgca
     6181 gcgcgtccac ggcgtcctcc gcgagcagcg aatggccgtg cttcgagacg aacaccgggt
     6241 cgaccaccag gttcg
//
LOCUS       DASPWO010000490         5303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_581508, whole genome shotgun sequence.
ACCESSION   DASPWO010000490 DASPWO010000000
VERSION     DASPWO010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581508"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VEO00_11170"
     CDS             complement(<1..1017)
                     /locus_tag="VEO00_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYU58597.1"
                     /translation="MAETYAYRVRDRAGRLQNGTLVADSEQLVLTRLREMGMVPISVE
                     VKKEGLSREIRVFGTGIKLKDLVVFSRQFATMINSGLSLLRSLAILEQQTENKRLGAV
                     LAEVRLDVERGASLSQALSKHPRVFNRLYVAMVRAGEAGGVLDTTLLRLADTLERELS
                     LRQRVKSAMTYPIVVAAFVMLILAAMLLFIVPQFKDIYTQLHGVLPLPTRILLFVSDV
                     FRKWWWVWILIVIGLIFAFRRFKKTPQGRAAVDAFKLKVPVFGGLFHKTALSRFSSTL
                     SILLRAGVPILQSLDIVTETVNNRVIARAVADVETSVKEGESIARPLGKHAVFPPMVV
                     QMLAV"
     gene            complement(1043..2179)
                     /locus_tag="VEO00_11175"
     CDS             complement(1043..2179)
                     /locus_tag="VEO00_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012087261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HYU58598.1"
                     /translation="MTQNVMTEVQTFGEQRPEVEISELLGLTIDRRASDLHLTAGTHP
                     VLRVNGSLIRMEQYPKLVPDELRRMIYAILTQKHRERLEEDLELDFSYSLPGRARFRG
                     NVYIQRGSLGAVFRLIPVEIRSVAELGLPEVVNDLARVPRGMVILTGPTGSGKSTTLA
                     AMIDLVNRERDCHIMTVEDPIEFLHQHKTAVVNQREVGSDTHGFAQALRHALRQDPDV
                     ILVGEMRDLETIATGLTAAETGHLVFATLHTQDAPQTIDRIIDVFPTAQQQQIRVQLA
                     STLQGVVTQQLLPRSDGSGRVVATEVMVATPAVRNLIREAKVHQIRTAMQAGARHGMH
                     TMDSSLADLVRAGKVAHPVAAERAHDVDELNRLLGRTVEGAPAR"
     gene            complement(2182..3849)
                     /locus_tag="VEO00_11180"
     CDS             complement(2182..3849)
                     /locus_tag="VEO00_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYU58599.1"
                     /translation="MTPLGALLVGAGLITSEQLDEALEEQQRSRGKLGRILIDRGLVR
                     EADLVRMLSRQVGLRFADLSEYPVDPAAAAAIPEAVARRYLAIGVGFEDGKLVVAMSD
                     PANVFALDDIRTITGREVQPVVATASDVEAAIRRYSRMDQAVEEFATEAAMAAEEDVD
                     AAAAGAAVEEGPIVKLVNLLIAQAVNDRASDIHVEPWERDVRIRYRIDGVLHEVMRSP
                     KNIQLGLTSRLKIMSDINIAERRIPQDGRVSLQVGGRSVDLRVATLPTVHGEKVVIRI
                     LDKSHALLRLDQLGFLEGPYRRYERAFRKPYGAILVTGPTGSGKSTTLYATLNIINTD
                     DRNIITVEDPVEYRLAGVNQIQVNPKAGLSFASALRSILRADPDVVLIGEIRDRETAM
                     IAVESALTGHLVLSTLHTNDAAAAITRLIEMGVEPYLVASAMDCIVAQRLARLLCDRC
                     KQPYTPDRADLEEAGFGEEDRDRIEVLYRAVGCPVCGKTGFRGRLGLYEVMPVTEEVE
                     RLTVARSSSEDIRRLAVEQDMVTLREDGLAKVAMGLTTIEEVLRVVV"
     gene            complement(3911..4513)
                     /locus_tag="VEO00_11185"
     CDS             complement(3911..4513)
                     /locus_tag="VEO00_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58600.1"
                     /translation="MTSRSDFAEIAARAAAWADDVRACLILSADGLTLGAFPAEQEAA
                     ARDVWSRLAGTGHPARGFLEFGTETWACVRRGGYSGVLIASVGARPGVLLDALDRALR
                     EAQGEATETPRAPEPPARRARPDVPRETKRIEHVLATRTEAPVTIPDSDAAGPQADAA
                     GVPEPVAVAAAGEEGPLPLEIDPVELSREFGALLDRDRER"
     gene            complement(4601..>5303)
                     /locus_tag="VEO00_11190"
     CDS             complement(4601..>5303)
                     /locus_tag="VEO00_11190"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HYU58601.1"
                     /translation="AVAGLAAAGVGGLNVTMPHKEAAVRVAEARSEDVERLRAANVLT
                     FDAGPVRADNTDVAGFARFLADDAGFDPRGASVLLFGAGGAARACALAVARGGAARLT
                     AAVRDPARARALREALDGTGVRVTVETFAGLSAGGADLVVNATPLGGAGEPLPASGLR
                     PGQTVVDLVYRPAVTPLVAGARAAGADAHGGLGLLLHQAALTVELWTGQAPPLDVMSA
                     AALAELAEDPRGLGG"
BASE COUNT          726 a         1999 c         1833 g          745 t
ORIGIN      
        1 cacggccagc atctgcacga ccatcggggg gaacacggcg tgcttaccca gcggccgggc
       61 gatcgactcc ccctccttca cgctggtctc gacgtccgcg acggcccgtg cgatgacccg
      121 gttgttgacg gtctcggtga cgatgtccag cgactgcagg atcggcactc ccgcgcgcag
      181 caggatcgac agcgtggacg agaaccgaga cagcgcggtc ttgtggaaca gcccgccgaa
      241 gaccgggacc ttcagcttga aggcgtcgac cgcggcgcga ccctggggcg tcttcttgaa
      301 ccggcggaac gcgaagatca acccgatgac gatcaggatc cagacccacc accacttccg
      361 aaacacgtca gagacgaaca gcaggatccg tgtcggcagc ggcagcacgc cgtgcagctg
      421 cgtgtagatg tccttgaact gcgggacgat gaacagcagc atcgccgcca ggatcagcat
      481 cacgaacgcc gccaccacga tcgggtaggt catcgctgac ttgacgcgct ggcgcaggga
      541 cagctcccgc tccagcgtgt cggccaggcg caacagtgtc gtgtcgagca cgccgccggc
      601 ctcgccggcg cggaccatcg ccacgtacag ccggttgaac acccggggat gcttcgagag
      661 ggcctgcgac agcgacgccc cgcgctcgac gtccagccgg acctccgcga gcaccgcccc
      721 caggcgcttg ttctccgtct gctgctcgag gatggccagc gagcgcagca gcgagagccc
      781 ggagttgatc atcgtggcga actgccgcga gaagacgacc aggtccttca gcttgatccc
      841 ggtcccgaac acgcggatct cgcgggacag gccctccttc ttcacctcca ccgagatcgg
      901 gaccatgccc atctcgcgga ggcgcgtcag gacgagctgc tcgctgtcgg cgaccagcgt
      961 gccgttctgc agccggccgg cccggtcgcg gacccggtag gcgtaggtct cggccatcgc
     1021 cgcgctcccc tcgtccctgc cgctaccgcg ccggcgcgcc ctcgacggtc cgtccgagga
     1081 ggcggttcag ctcgtcgacg tcgtgcgcgc gctcggcggc caccgggtgg gcgaccttcc
     1141 cggcacggac caggtcggcc agcgacgagt ccatcgtgtg catgccgtgg cgggccccgg
     1201 cctgcatcgc ggtgcggatc tggtggacct tcgcctcgcg gatcaggttc cgcaccgccg
     1261 gcgtggcgac catcacctcg gtggccacca cccggccgct cccgtcggac ctcgggagca
     1321 gctgctgggt cacgacgccc tggagcgtcg acgcgagctg cacccggatc tgctgctgct
     1381 gcgcagtggg gaacacgtcg atgatccggt cgatcgtctg cggcgcgtcc tgcgtgtgca
     1441 gcgtggcgaa gaccaggtgg ccggtctcgg ccgccgtcag gccggtggcg atcgtctcca
     1501 gatcgcgcat ctcgccgacc aggatcacgt cggggtcctg gcgcagggcg tggcgcagcg
     1561 cctgggcgaa cccgtgcgtg tccgagccga cctcgcgctg gttcaccacc gcggtcttgt
     1621 gctggtggag gaactcgatc gggtcctcca cggtcatgat gtggcagtcg cgttcccggt
     1681 tgaccaggtc gatcatcgcc gcgagcgtcg tcgacttccc ggagccggtc ggtccggtga
     1741 ggatgaccat cccccgcggc acgcgggcca ggtcgttcac cacctccggc agcccgagct
     1801 ccgccaccga ccggatctcg accgggatca gccggaacac cgcgccgagc gagccgcgct
     1861 ggatgtagac gttgccccgg aagcgcgccc gcccggggag gctgtaggag aagtcgagct
     1921 ccaaatcctc ctcgagccgc tcgcggtgtt tctgcgtcag gatcgcgtag atcatccgcc
     1981 gaagttcgtc cgggacgagt ttcgggtact gctccatccg gatcaggctg ccgttgacgc
     2041 gcagcaccgg atgcgtgccg gcggtgaggt ggaggtcgct cgcccggcgg tcgatggtca
     2101 ggccgagcag ctcggagatc tccacctcgg ggcgctgctc gccgaaggtc tgcacctcgg
     2161 tcatcacgtt ctgggtcata cctacaccac cacccgcagg acctcttcga tggtcgtcag
     2221 gcccatggcc accttcgcca ggccgtcctc ccgcagcgtg accatgtcct gctcgacggc
     2281 cagccggcgg atgtcctcgg acgaggagcg cgccaccgtc agccgctcca cctcctccgt
     2341 gaccggcatg acctcgtaga ggcccagccg gccgcggaat cccgtcttcc cgcacaccgg
     2401 gcagcccacc gcccggtaga ggacctcgat gcggtcccgg tcctcttcgc cgaaccccgc
     2461 ctcctccagg tcggcgcggt ccggggtgta gggctgcttg caccggtcgc acagcagccg
     2521 ggcgaggcgc tgggcgacga tgcagtccat ggcgctcgcc accaggtagg gctcgacccc
     2581 catctcgatc agccgcgtga tcgccgcggc cgcgtcgttc gtgtgcagcg tcgagagcac
     2641 caggtggccg gtgagtgccg actcgaccgc gatcatcgcg gtctcgcggt cgcggatctc
     2701 cccgatcagt acgacgtcgg ggtcggcccg caggatcgac cgcagcgccg aggcgaacga
     2761 cagcccggcc ttggggttca cctggatctg gttcacgccc gccaggcggt actccaccgg
     2821 gtcctcgacc gtgatgatgt tccggtcgtc cgtgttgatg atgttcagcg tggcgtacag
     2881 cgtcgtcgac ttgcccgagc cggtcgggcc ggtgaccagg atcgcgccgt acggcttgcg
     2941 gaacgcccgc tcgtagcgcc ggtagggccc ctccaggaac ccgagctgat ccaggcgcag
     3001 gagcgcgtgg ctcttgtcca ggatccggat gacgaccttc tcgccgtgga cggtcggcag
     3061 cgtcgccacc cgcaggtcca ccgagcgccc cccgacctgc aggctcacgc ggccgtcctg
     3121 cgggatccgc cgctcggcga tgttgatgtc ggacatgatc ttcagccgcg aggtcagccc
     3181 gagctggatg ttcttcggcg accgcatcac ctcgtgcagc actccgtcga tccggtagcg
     3241 gatccgcacg tcgcgttccc acggctcgac gtggatgtcc gatgcccgat cgttgacggc
     3301 ctgggcgatg agcaggttga cgagcttgac gatcgggccc tcctccaccg ccgcgccggc
     3361 cgcggccgcg tcgacgtcct cctccgccgc catcgccgcc tcggtggcga actcctcgac
     3421 cgcctggtcc atccgcgagt agcgccggat cgccgcctcg acgtcactgg ccgtggccac
     3481 caccggctgg acctcgcggc cggtgatcgt gcggatgtcg tcgagggcga acacgttcgc
     3541 cgggtccgac atcgccacga ccagcttgcc gtcttcgaac ccgaccccga tcgccaggta
     3601 gcggcgagcc accgcctcgg ggatcgcggc cgcggcggcc gggtcgaccg ggtactcgga
     3661 gaggtcggcg aaccgcaggc ccacctggcg agagagcatc cggacgaggt cggcctcccg
     3721 gaccaggccc ctgtcgatca ggatccggcc gagcttgccg cgcgagcgct gctgctcctc
     3781 gagcgcctca tcgagctgct ccgaggtgat caggccggcc ccgaccagga gcgcgccgag
     3841 cggggtcacc ccgctcgagg cgcgttgcac ccgctcgggg gcctgcatgg ttcgctcgtc
     3901 ggcgcgcacg tcagcgctcc cgatcgcggt cgaggagcgc gccgaactcc cgggagagct
     3961 cgaccgggtc gatctccagg ggcagcggtc cctcctcgcc ggccgcggca acggcgacgg
     4021 gctccgggac gccggccgcg tcggcttggg ggccggccgc gtccgaatcg gggatggtca
     4081 cgggcgcctc ggtgcgggtg gccagcacgt gctcgatgcg cttcgtctcc cgggggacgt
     4141 ccgggcgcgc ccggcgggcc ggcggctcgg gcgcccgggg cgtctccgtc gcctcaccct
     4201 gcgcctcgcg cagcgcccgg tccagggcgt cgaggaggac ccccggccgc gcgccgaccg
     4261 acgcgatcag gacgccggag tagccccccc gccggacgca cgcccaggtt tcggtgccga
     4321 actccaggaa cccgcgggcc gggtggccgg tcccggccag gcggctccac acgtcgcgcg
     4381 cggccgcctc ctgctcggcc gggaacgcgc cgagggtcag gccgtcggcg gacaggatca
     4441 ggcacgcgcg cacgtcgtcc gcccacgccg ccgcccgcgc ggcgatctcc gcgaaatccg
     4501 atctcgaggt catggcgctc cccacctgtt tcggaacgcc ccgtgcccac cttgagcccc
     4561 gaatgggctc aaaggggcgg cccaccgcac tcgggccgag ctaccccccc aaccctcggg
     4621 ggtcctcggc cagttcggcg agggccgccg cggacatcac gtcgaggggc ggtgcctggc
     4681 cggtccacag ctcgacggtc agggcggcct ggtgcagcag caggccgagc ccgccgtgcg
     4741 cgtccgcgcc ggccgcccgg gcgccggcga ccagcggcgt caccgccggg cggtacacga
     4801 ggtccaccac cgtctggccg gggcgcagac cgctcgccgg cagcggctcg ccggcgccgc
     4861 ccagcggcgt ggcgttcacc accaggtcgg cacccccggc cgacaggccc gcgaacgtct
     4921 ccacggtgac tcgcaccccc gtgccgtcga gcgcctcgcg cagcgcccgg gcgcgcgccg
     4981 ggtcgcgcac cgccgcggtg agccgcgccg ccccgccccg cgccaccgcc agcgcgcagg
     5041 cgcgggccgc ccccccggcg ccgaacagga gcaccgaggc cccgcggggg tcgaacccgg
     5101 cgtcgtcggc gaggaaccgc gcgaagcccg cgacgtcggt gttgtcggcg cgcaccggcc
     5161 cggcgtcgaa cgtcaggacg ttcgcggcgc gcagccgctc cacgtcctca gagcgggcct
     5221 cggccacgcg cacggccgcc tccttgtgcg gcatcgtcac gttcaggccg ccgacgccgg
     5281 ccgcggccag gccggcgacc gcc
//
LOCUS       DASPWO010000491        12059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1254222, whole genome shotgun sequence.
ACCESSION   DASPWO010000491 DASPWO010000000
VERSION     DASPWO010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12059
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254222"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2840)
                     /locus_tag="VEO00_11195"
     CDS             complement(<1..2840)
                     /locus_tag="VEO00_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HYU58602.1"
                     /translation="MDRLYDPRHERDACGIGFVADAAGRPSRAIVETALEALRRVRHR
                     GALAADARTGDGAGLLVPLPRRFFETLAPVAGSGPLGTAMALLDARDPAPGRAIVEAA
                     CAAEGIAVVAWRDVPVDPAALGDGARQSLPALVQALLVPRRGAGPEAAERAAFRARRR
                     AEAAAREARARCYLASLSFRTVVYKALCAADQLSAFYADLADPAFEAPFAVFHQRYST
                     NTTPTWERAQPFRFLCHNGEINTIQGNVNLMRAREGRLGAGDLAPEELLAPVIDPDGS
                     DSAMLDNALELLVRGGRDVRHALAMLVPEAWEGLPLPEGLRDFYRYHACLVEPWDGPA
                     GLVFTDGVRVGATLDRNGLRPLRYAVCQDGLVACASEAGAVDVAGHGTVRRGMLGPGQ
                     MIAVDPGAGGFLENDVIKRSLAAAGPWGAWGAALRPADPGPPVPHPGPALAERQVAHG
                     FDKEEVTVVIRPMATQGHEPTSSMGDDTQQAVIAERRRTVQHFLRQRFAQVTNPPIDH
                     LRERLVMATRTVLGPRAPILSDGPEAAALLELPGFLLTPAGLEALVAAAPWPAATLDA
                     TFPVAEGPDGLRAAVERLGREAEVDVAGRAALLVVSDWATGPERAPVPSILAVGAVHH
                     ALLRAGLRTRASIVAATDEPRDVHAVACLLGYGAEAVCPRLVLETIADICVEGRMGGD
                     SPPAEVAARRYVEAMEDGVLKVMSKMGIATVSSYRSAQIFEAVGLAPEVIEACLAGTP
                     SPIGGIGFADLGADVLARHRAAFTARPVLVNPGFVKHRAGGDYHATNPEVVGALHAVV
                     GVDRDPRAGGGEGPDGEPVPATAHALQRAVRGGGYEDYERFAALVNERPASEPRDLLE
                     ALPAGPPIPLEEVEPAAAITRRFSTGAMSHGALSAEAHETLAVALRMVGGKANTGEGG
                     EARARFRDERNSGIKQVASGRFGVTPEYCAFA"
     gene            complement(3015..3902)
                     /locus_tag="VEO00_11200"
     CDS             complement(3015..3902)
                     /locus_tag="VEO00_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HYU58603.1"
                     /translation="MGATFLIMLREGLEIALVLSIVLAYLNKSGSRERHATVWAGAGT
                     AAAVSIAAGAIIFAVAGELPHRAGEVFEGVATLLAVGVLTWMLFWMRRNAAFLRGKLQ
                     HRVDVALEEGSSITLAAVAFFVVLREGLETALFLFSSLRATSDTQPALAFTGALLGLA
                     GAAVLGYLFYRGGIRLNLRVFFRVTGLLLLVVAATLLVYGLHELTDAGAFRALEDTFL
                     LGGAGAAAIPLKAVGKALVGLGGGRTWLELVAWAGYLLVVGGLFLRPVPAPAAPPAAR
                     ATTGDEPDTQASDRAARST"
     gene            complement(3988..4662)
                     /locus_tag="VEO00_11205"
     CDS             complement(3988..4662)
                     /locus_tag="VEO00_11205"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HYU58604.1"
                     /translation="MTAPQALTASDYLEALYEMIEEDIPAQQARLAEWMGVSRASVSE
                     AVKRLVRDGLLTSEDRTLAFTRRGLSASESLVRRHRLAERFLIEVIGLRWHLAHEEAT
                     RWGHVVSDQVEERLVEILGDPATCPHGNPIPGSSHPVDHSDLRPLNDFGPGARVRLER
                     LTEDLELELEVMRFFEESGLMPGADIAVESVAPDGTMTLSVAGHGSALGAHLADNLWV
                     RPAISS"
     gene            4686..7322
                     /gene="polA"
                     /locus_tag="VEO00_11210"
     CDS             4686..7322
                     /gene="polA"
                     /locus_tag="VEO00_11210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HYU58605.1"
                     /translation="MASPRLFLLDGHSLAFRAFFALPSDLATTSGQVTNAVYGFTSML
                     IKLLGEERPDLLAVAFDVGKPTVRLEKYAEYKAGRAEAPDEFRQQLGLIVEVLETLRV
                     PVLGIPGHEADDVIATLAVRAAEEGIDVTIVTADRDFFQLVRPGITVMFNRRGISDIV
                     RYDAAAVTERFGLPPEKYLDYVALKGDTSDNIPGIAGVGEKTASRVIQEFGSVEELLR
                     RLDEAPPKIRAAVEAGHDLALNKDLARLVTDLDLDARPEDLALGEWDPEAVRRLFNSL
                     EFRTLLERLEDLGRTAKPALESADLDLSQGAERDLGRLLAGDAPRAVTLAGEPGRIEA
                     IAVSAGGGQAVHVAVQGVPKAAAAFFADPGRPKWVHDAKELATLLEASGVRPAGVAFD
                     TMLAGYLLDPAETGYALGALTERYLGVDVVSSSEDEGAGKTGQLFGPDWRAVAAQAAA
                     VALLAPLLAERVERAGLKGLLEDVELPLAQVLARMQAAGVAIDVPYLQEMSETLGDRM
                     ATLEQEIYRHAGEPFNLNSPPQLRVVLYERLGLMPSKRTSKGALSTDASVLEKLREQH
                     PIIDALLQYREYSKLKSTYLDALPPLVDPRDGRLHTTYVQTAATTGRLSSTNPNLQNI
                     PIRGELGRQIRRAFIPGSPGEVLFVADYSQIELRVLAHLSGDENLARAFASDTDIHLA
                     TAATIAGLPPEAVDPEWRRRAKMVNYGLAYGMSAYGLAQRLGIAPDEAQEFIDAYFAG
                     FPKVREFLDRQVATATTEGFTATILGRRRYLPELQSANPRMRDMGRRMALNAPIQGTA
                     ADIMKLGMIRVDEALEASGLPARMVLTVHDELVFEVPQGSVDKVSDLVKREMEAAYPL
                     EVPLRVDLGSGANWADAAPAGH"
     gene            7349..7942
                     /locus_tag="VEO00_11215"
     CDS             7349..7942
                     /locus_tag="VEO00_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HYU58606.1"
                     /translation="MMPPTAIRARPHRFTVSEYQRMGEAGILGEDDRVELIEGEIVEV
                     AAIGRRHASTVDRLTALFVNSFGDRAHVAVQNPVVLSDITEPQPDVVLLRPREDFYAG
                     ADRDPEATLLVVEVAATSLGYDGGVKFPLYARAGVPEVRIVDLQGRRVERHLGPGPAG
                     YADSRGLDMGERLAPTAFPDVEVGVEEILGPEPDPAP"
     gene            complement(7945..9732)
                     /locus_tag="VEO00_11220"
     CDS             complement(7945..9732)
                     /locus_tag="VEO00_11220"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HYU58607.1"
                     /translation="MSSDDFDLQDLAEHWQQQFAKPENLQRMRQAFQAFGATLQGGRM
                     PSIRQSISLARTLGTIGQPRRMFGAGTVIVTTLIALGVLAGVFGPVLKTDFGLPTLPV
                     TKRLFPQASGFSAADVEAVLAPDGTVAVRESVHLEDGGSVSLLGPCPADGSVPTVACV
                     RDVQVDGRPVAPAASGSGVSVQIDGEDAVLSYTADGLAMRFTDVSILAWPVLPQAFGN
                     TGPFDPRVTLRGRLRLPRAPEAPSEIHLHSAGERSARLEGDAVVFEGDTALYIRAIDL
                     QAAFPAGLLPDIPDERTFGGPGILGFRIQQQVEDQLDASFAAVPGEVTTPREVFNLII
                     FAFAVAPALALWILVLVRLVRRITHRRPKLDLPKYEEQPPTNHDPAVVAALAAEGKLP
                     PEAVAGAVLSLAERKRLDIQDLPGEHFVMRVVPGAIGDTPGEGLLLAVLASKAAQTGG
                     KIEGPPLWDGKVGFWKSFRKDALRRVARLGLAERADTTSLAGVALIVTIGSILVTFLL
                     PSPFLFFTFAVMLPILAAVVTFRLGYALTEEGRLLRERWLAFGRHVRERTQMGNVPPA
                     GVVIWGPYLTYGTVLDAAPKAAEQLSPQV"
     gene            9949..11319
                     /gene="rpsA"
                     /locus_tag="VEO00_11225"
     CDS             9949..11319
                     /gene="rpsA"
                     /locus_tag="VEO00_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HYU58608.1"
                     /translation="MTAGAEAPSDDYTPEELARAIEESLKDFKDGDLVSGKVVRIDRD
                     EVLLDIGYKSEGVIPAKELSIRHDVDPDQVVKVGDEIEALVLQKEDKEGRLILSKKRA
                     QYERAWGRIEEIMREGRTITGPVIEVVKGGLILDIGLRGFLPASLVDLRRVRDLHPFV
                     GQELEAKIIELDRNRNNVVLSRRAFLEESQSEGRKKFLESLQKGERRTGTVSSIVNFG
                     AFVDLGGVDGLVHVSELSWKHVDHPSEVVSVGQEVEVEVLDVELERERVSLSLKATQE
                     DPWREFERNHKAGEVISGQVTKLVPFGAFVRVGEGIEGLVHISELSDAHVDMPEKVVS
                     VGQQVRVKVVDVDVSRRRISLSMRQVGEQEPVAEIAPEPSPVAEVAEPEAEAVVPAGP
                     PPTVEEAAAAEAEAMAVPEDAPPVEPEPEPASPAAPEPPAEEPTDEDVSLESIVEDLK
                     RREGRS"
     gene            11486..>12059
                     /gene="coaE"
                     /locus_tag="VEO00_11230"
     CDS             11486..>12059
                     /gene="coaE"
                     /locus_tag="VEO00_11230"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HYU58609.1"
                     /translation="MLLVGLTGGIGTGKSTVAARLAERGAVVVDADVLGREAVAPGTA
                     GFENVVKRFGKRLVDPSTGGLDRNALAATVFADEMARRDLEAIIHPEVGRRLAETVGR
                     YRDSDAILVFDAPLIVEGGFADMVDVLVVVTSKAEEQVRRLMELRGMAEDDVRARIGA
                     QAPTEEKVARADVVIENDGSEADLAERVDAL"
BASE COUNT         1740 a         4284 c         4484 g         1551 t
ORIGIN      
        1 tcggcgaacg cgcagtactc gggcgtcacg ccgaaccgcc cggacgcgac ctgcttgatg
       61 cccgagttgc gctcgtcgcg aaagcgagcg cgcgcctcgc ccccctctcc ggtgttcgcc
      121 ttgcctccca ccatccgcag cgccaccgcc agcgtctcgt gcgcctccgc cgacagcgcg
      181 ccgtgcgaca tcgccccggt cgagaaccgc cgcgtgatcg ccgcggccgg ctcgacctcc
      241 tcgagcggga tcggcgggcc cgccgggagc gcttccagca ggtcgcgcgg ctccgacgcc
      301 ggccgctcgt tgaccagtgc ggcgaaccgc tcgtagtcct cgtagccgcc gccccgaacc
      361 gctcgctgca gcgcgtgcgc cgtggcgggg accggctcgc cgtccggccc ttcgccgccc
      421 ccggcccgcg ggtcgcggtc cacgccgacc accgcgtgga gggcgccgac cacctccggg
      481 ttcgtggcgt ggtagtcgcc cccggcgcgg tgcttcacga atcccgggtt gaccaggacc
      541 gggcgggcgg tgaaggcggc ccggtgccgg gccaggacgt cggcgccaag gtcggcgaac
      601 ccgatccctc cgatggggga gggggtcccg gccaggcagg cctcgatgac ctcgggggcc
      661 agcccgacgg cctcgaagat ctgcgcgctg cgataggagg agacggtggc gatgcccatc
      721 ttcgacatga ccttcagcac gccgtcctcc atcgcttcca cgtagcggcg cgccgccacc
      781 tcggccggcg ggctgtcgcc gcccatccgc ccttcgacgc agatgtcggc gatcgtctcc
      841 aggaccaggc gcggacagac ggcctctgcg ccgtagccga gcaggcaggc cacggcgtga
      901 acgtcgcgcg gctcgtcggt ggcggcgacg atcgaggcgc gcgttcgcag accggcccgc
      961 aggagggcgt ggtgcaccgc gccgacggcg aggatcgagg ggaccggcgc gcgctcaggc
     1021 ccggtggccc agtccgacac gacgagcagg gcggctcggc ccgccacgtc cacctcggcc
     1081 tcccgcccga gacgctccac ggccgcccgc aggccgtccg ggccctcggc caccgggaag
     1141 gtcgcgtcga gggtcgccgc cggccagggt gccgccgcca ccagagcctc cagaccggcc
     1201 ggggtcagga ggaacccggg cagttcgagg agggcggcgg cctcggggcc gtcggagagg
     1261 atcggcgccc gcgggccgag caccgtccgt gtcgccatca ccagccgctc gcgcaggtgg
     1321 tcgatcgggg ggttggtcac ctgggcgaag cgttgccgta ggaagtgctg cacggtccgg
     1381 cggcgctcgg cgatcaccgc ctgctgcgtg tcgtcgccca tcgacgacgt cggctcgtgc
     1441 ccctgggtgg ccatcgggcg gatgaccacc gtgacctcct ccttgtcgaa cccgtgggcc
     1501 acctggcgct cggcgagcgc ggggccggga tgcgggacgg gcggccccgg gtccgccggc
     1561 cgaagggcgg cgccccacgc gccccacggc ccggccgccg ccaacgaccg tttgatcacg
     1621 tcgttctcga ggaagccgcc ggcgcccggg tcgaccgcga tcatctggcc gggaccgagc
     1681 atcccgcggc ggaccgtccc gtgcccggcc acatcaaccg ccccggcctc cgaggcgcag
     1741 gccaccagcc cgtcctggca gacggcgtag cgcagcgggc gcaggccgtt gcggtccagg
     1801 gtcgccccga cccgcacgcc gtcggtgaac accagccccg ccggcccgtc ccacggctcc
     1861 accaggcagg cgtggtagcg gtagaagtcc cgcaggccct ccgggagcgg cagcccctcc
     1921 caggcctcgg ggacgagcat cgccagcgcg tgacggacgt cgcggccgcc gcgaacgagc
     1981 agctcgaggg cgttgtcgag catcgccgag tccgagccgt cggggtcgat caccggcgcc
     2041 agcagctcct cgggagccag gtcgccggcg ccgagccgcc cctccctggc gcgcatcagg
     2101 ttgacgttcc cctggatcgt gttgatctcg ccgttgtggc acaggaatcg gaacggctgg
     2161 gcccgctccc aggtcggggt ggtgttcgtc gagtagcgct ggtggaagac cgcgaacggc
     2221 gcctcgaagg cggggtcggc caggtcggcg tagaacgcgg agagctggtc ggccgcgcag
     2281 agcgccttgt agacgaccgt ccggaacgag agcgaagcca ggtagcagcg agcccgggcc
     2341 tcccgcgcgg cggcctcggc ccggcgccgg gcccggaacg ccgcccgctc cgccgcctcg
     2401 ggaccggcgc ctcgccgggg gacgagcagc gcctggacca gtgcgggcag cgactggcgg
     2461 gccccgtcgc cgagggccgc cggatcgacc ggcacgtcgc gccacgcgac gacggcgatc
     2521 ccttcggcgg cgcaggcggc ctcgacgatc gcccggcccg gcgccgggtc ccgggcgtcg
     2581 aggagagcca tggccgtccc gagtggcccg ctgccggcga cgggggcgag cgtctcgaag
     2641 aaccgccggg ggagcgggac caggagcccg gcgccgtcac cggtgcgcgc gtccgcggcg
     2701 agcgcgccgc ggtggcggac gcggcgcagc gcctcgagcg ccgtctcgac gatcgcccgg
     2761 gatggccggc cggcggcatc ggcgacgaag ccgatcccgc aggcgtcgcg ctcgtggcgc
     2821 gggtcgtaca ggcggtccac gcggtcggtc ctctccctgg tggccggaaa cggatcgcgg
     2881 ccgggcggcg cgcccggcca cgaagaaggc gccccgccgt cggcgaagcg cccttgcttc
     2941 tcgctatggg tgttgcgctg agggtaccgg tcggatggcg gcctcgcaac cacgcgacgc
     3001 cgcgctgcgg cccgctaggt cgaacgggcc gcccggtcgc tcgcctgagt gtcgggctcg
     3061 tcgccggtcg tggcccgagc ggccggcgga gcggccggtg ccgggacggg gcgcaggaag
     3121 agcccgccga cgacgagcag gtacccggcc caggcgacga gctcgagcca cgtccggccg
     3181 ccccccaggc cgacgagcgc cttgcccacg gccttcagcg ggatcgcagc ggcgccggcc
     3241 ccaccgagca ggaacgtgtc ctccagggcg cggaaggcgc cggcgtcggt cagctcgtgc
     3301 aggccgtaga ccagcagggt ggccgccacg accagcagga gcaggcccgt gacccggaag
     3361 aacacgcgca ggttgaggcg gatgcccccc cggtagaaga ggtacccgag caccgcggcg
     3421 ccggccagcc cgaggagggc gccggtgaag gccagggcgg gctgcgtgtc ggacgttgcc
     3481 cgcagggagc tgaacaggaa gagcgcggtc tccaggccct cgcgcaggac cacgaagaat
     3541 gccaccgcgg ccagcgtgat cgacgacccc tcctcgagcg cgacgtcgac ccggtgctgc
     3601 agcttccccc ggaggaaggc ggcgttccgc cgcatccaga agagcatcca ggtcaacacg
     3661 ccgacggcca agagggtggc gacgccctcg aagacctcgc cggcccggtg aggaagctcg
     3721 ccggcgaccg cgaagatgat cgcgccggcc gcgatgctga ccgccgcggc ggtcccggcg
     3781 ccggcccaca cggtggcgtg gcgttcgcgg ctgccggact tgttcaggta ggcgaggacg
     3841 atcgagagga ccagggcgat ctccagcccc tcgcggagca tgatgaggaa cgtcgcgccc
     3901 atccccctgc ctcccgcccc tgttgttcgg gttaccttac atccatcgtt cgggccgccg
     3961 aatccaagtg taccagtcgg attcgagtca agaggagatc gccgggcgga cccagaggtt
     4021 gtcggcaagg tgggcgccga gggccgagcc gtgcccggcc accgacaggg tcatcgtccc
     4081 gtccggggcc accgactcga ccgcgatgtc ggcccccggc atcaggccgg actcctcgaa
     4141 gaaccgcatc acttcgagct ccagctcgag gtcctccgtc agccgctcca ggcggacgcg
     4201 ggcgccgggg ccgaagtcgt tcagcggacg aaggtcggaa tggtccaccg ggtgcgagga
     4261 cccggggatc gggttgccgt gcggacaggt ggccgggtcg ccgaggatct ccaccagccg
     4321 ctcctcgacc tggtcggaga cgacgtgccc ccagcgggtc gcctcctcgt gggccaggtg
     4381 ccaacgcagg ccgatcacct cgatcaggaa gcgctccgcc agtcggtggc ggcggacgag
     4441 tgactcggag gccgagagcc cccgccgggt gaaggccagc gtgcggtcct cgctcgtcag
     4501 cagaccgtca cgcaccaggc gcttcaccgc ctccgagacg gaggctcgcg aaacgcccat
     4561 ccactccgcc agccgggcct gctgggcggg gatgtcctcc tcgatcatct cgtagagcgc
     4621 ctcgaggtag tccgacgcgg tgagcgcctg aggggccgtc acggggcgag tctacaatcc
     4681 acgtcgtggc ctccccccgg ctgttcctgc tcgacggaca cagcctcgcg ttccgcgcct
     4741 tcttcgccct gccgtcggac ctggcgacga ccagcgggca ggtgacgaac gcggtctacg
     4801 gcttcacctc gatgttgatc aagctgctcg gcgaggagcg cccggatctg ctggccgtgg
     4861 ccttcgacgt cggcaagccg accgtgcgcc tggagaagta cgccgagtac aaggccgggc
     4921 gggcggaggc gccggacgag ttccgccagc agctcgggct gatcgtcgag gtgctcgaga
     4981 cgctgcgcgt gccggtgctc gggatccccg ggcacgaggc cgacgacgta atcgccacgc
     5041 tcgcggtccg cgccgccgag gaggggatcg acgtgacgat cgtcaccgcc gaccgcgact
     5101 tcttccagct ggtgcggccg gggatcacgg tgatgttcaa ccgtcgcggc atctccgaca
     5161 tcgtccgcta cgacgccgcc gcggtgaccg agcgcttcgg gctgccaccg gagaagtacc
     5221 tcgactacgt ggccttgaag ggggacacga gcgacaacat ccccggcatc gccggcgtcg
     5281 gcgagaagac cgcgtcgagg gtgatccagg agttcgggtc ggtcgaggag ctgctccgcc
     5341 ggctggacga ggcgccgccg aagatccgcg cggcggtgga ggccggccac gatctggcgc
     5401 tgaacaagga cctggcccgc ctggtgacgg atctggacct cgacgcccgg ccggaggacc
     5461 tggcactggg ggagtgggac cccgaggccg tccgccggct cttcaacagc ctggagttcc
     5521 gcacgttgct cgagcgcctg gaggacctcg ggcgcaccgc gaagccggcg ctcgagtcgg
     5581 ccgacctgga cctgtcgcag ggcgccgagc gcgacctcgg gcgcctcctg gccggcgacg
     5641 ccccgcgagc ggtcacgctg gccggggagc ccggacggat cgaggcgatc gccgtctcgg
     5701 ccggcggggg ccaggccgtg cacgtcgccg tgcagggggt gccgaaggcg gcggcggcgt
     5761 tcttcgcgga ccccggccgt ccgaagtggg tgcacgacgc caaggagctc gccaccctcc
     5821 tggaggcctc cggtgtccgg ccggccgggg tggcgttcga cacgatgctc gccgggtacc
     5881 tgctcgaccc ggccgagacc ggctacgcgc tcggcgcgct gaccgagcgc tacctgggcg
     5941 tggacgtcgt ttcctcctcg gaggacgaag gtgcggggaa gacggggcag ctgttcggcc
     6001 ccgactggcg ggccgtggct gcgcaggccg cggcggtggc gctgctcgcg ccgctgctcg
     6061 cggagcgcgt cgagcgcgcc ggcctgaagg gcctgctgga ggacgtcgag ctgccgctgg
     6121 cgcaggtgct ggcgcggatg caagcggccg gcgtggcgat cgacgtcccg tacctgcagg
     6181 agatgtccga gacgctcggc gatcggatgg ctaccctcga gcaggagatc taccggcacg
     6241 ccggcgagcc gttcaacctg aactcgccgc cgcagctgcg ggtggtgctc tacgagcgcc
     6301 tcggcctgat gccgagcaag cgcacctcga agggcgcgct gtcgaccgac gcgagcgtcc
     6361 tggagaagct gcgcgagcag cacccgatca tcgacgcgct cctgcagtac cgggagtact
     6421 ccaagctgaa gagcacgtac cttgacgcgc tccccccgct ggtcgacccg cgcgacgggc
     6481 gcctacacac cacctatgtc cagacggccg ccaccaccgg ccggctctcc tcgacgaacc
     6541 cgaacttgca gaacatcccc atccggggcg agctcggtcg gcagatccgc cgcgccttca
     6601 tcccgggctc gccgggcgag gtactgttcg tcgccgacta ctcgcagatc gagctgcgcg
     6661 tgctggcgca cctgtccggc gacgagaact tggcgcgggc cttcgcctcg gacaccgaca
     6721 tccacctggc caccgcggcg acgatcgccg ggctgccgcc ggaggcggtc gatccggagt
     6781 ggcgccggcg ggcgaagatg gtgaactacg gactggcgta cgggatgagc gcctacggcc
     6841 tggcgcagcg gctcgggatc gcccccgacg aggcccagga gttcatcgat gcgtacttcg
     6901 cgggattccc gaaggtccgc gagttcctgg accgccaggt cgccaccgcc acgaccgagg
     6961 ggttcaccgc gaccatcctc ggacggcggc gctacctccc ggagctgcag tcggccaacc
     7021 cgcggatgcg ggacatgggc cggcggatgg cgctgaacgc gccgatccag gggacggccg
     7081 ccgacatcat gaagctcggc atgatccggg tggacgaggc gctggaggcg tcgggccttc
     7141 cggcgcggat ggtgctgacc gtgcacgacg agcttgtctt cgaagtgccg cagggttcgg
     7201 tcgacaaggt ctcggacctg gtgaaacggg agatggaagc ggcctacccg ctggaggtcc
     7261 cgctgcgcgt ggacctcggc tccggcgcca actgggccga cgccgccccc gcgggacact
     7321 gaggccggag gtgtcggagg ggctcggtat gatgcccccg acggccatcc gagcccggcc
     7381 acatcggttc acggtttccg agtaccagcg gatgggcgag gccggcatcc tcggcgagga
     7441 cgaccgggtc gagctcatcg agggggagat cgtcgaggta gcggcgatcg gccggaggca
     7501 tgcgtccacc gtggatcgcc tcaccgccct gttcgtgaac agtttcgggg atcgggccca
     7561 cgtggcggtg cagaacccgg tcgtgctttc cgacatcacc gagccccagc cggacgtggt
     7621 cctgctgcgg ccccgggagg acttctacgc gggggcggac cgcgatccgg aggccacgct
     7681 gctggtcgtg gaggtggcgg caacctccct cggctacgac ggcggggtga agttcccgct
     7741 gtacgcgcgg gccggggtgc ccgaggtccg gatcgtcgac ctccaggggc gccgggtcga
     7801 gcgccacctc ggtcccgggc ccgcgggtta cgcggactcc cgcggcctcg acatgggcga
     7861 gcgtctcgcg cccaccgcgt tcccggacgt cgaggtgggg gtcgaggaga tcctcggccc
     7921 ggagcccgac cccgcgccgt aacctcacac ttgcggcgag agctgctcgg cagccttcgg
     7981 ggcggcgtcg aggacggtgc cgtaggtcag gtacgggccc cagatcacca cgccggcggg
     8041 cgggacgttg cccatctgcg tgcgctcccg gacgtggcga ccgaaggcca gccagcgctc
     8101 ccggagcagc cgcccctcct cggtcagcgc gtagccgagc cggaacgtca ccaccgccgc
     8161 caggatcggc agcatcacgg cgaacgtgaa gaacaggaac gggctcggca gcaggaacgt
     8221 gaccaggatc gagccgatcg tgacgatcag cgccacaccg gccaggctgg tggtgtcggc
     8281 ccgctcggcc aggccgaggc gggcgacgcg ccgcagtgcg tccttgcgga acgacttcca
     8341 gaacccgacc ttcccgtccc acagcggtgg accctcgatc ttcccgccgg tctgggcggc
     8401 cttcgacgcg agcaccgcca gcaacagccc ctcccccggc gtgtcgccga tcgcccccgg
     8461 caccacgcgc atgacgaagt gctccccggg gaggtcctgg atgtccaagc gcttgcgctc
     8521 ggccagggag agcacggccc cggcgaccgc ctctggcggc agcttcccct cggcggccag
     8581 cgcggccacc acggcgggat cgtggttcgt cggcggctgc tcctcgtact tcggcaggtc
     8641 gagcttcggg cggcggtggg tgatccgccg gaccagcctg acgagcacca ggatccagag
     8701 cgccagggcc ggcgccacgg cgaaggcgaa gatgatcagg ttgaagacct cgcgcggcgt
     8761 cgtcacctcc ccgggcaccg cggcgaacga cgcgtccaac tggtcctcca cctgctgctg
     8821 gatccgaaag cccaggatcc cggggccgcc gaaggtccgc tcgtccggga tgtccggcag
     8881 gaggccggcc gggaacgcgg cctgcaggtc gatcgcgcgg atgtagaggg cggtgtcgcc
     8941 ctcgaacacg acggcgtcac cctcgaggcg cgcgctgcgc tcgccggccg agtgcagatg
     9001 gatctcgctc ggggcctcgg gcgcgcgcgg caggcgcagc cgcccgcgca gggtcacgcg
     9061 cgggtcgaac ggaccggtgt tcccgaacgc ctgcggcagg accggccacg ccaggatcga
     9121 gacgtcggtg aaccgcatgg ccagcccgtc ggcggtgtag ctcaggaccg cgtcctcgcc
     9181 gtcgatctgg acgctcaccc ccgaccccga ggcggcgggc gcgaccggcc gcccgtccac
     9241 ctggacgtcg cgcacgcagg ccacggtggg aaccgacccg tcggcggggc aggggccgag
     9301 caggctgacg gaccccccgt cctcgaggtg gacggactcg cgcacggcaa cggtgccgtc
     9361 gggcgccagc accgcctcca cgtcggccgc cgagaacccg ctcgcctggg ggaacagtcg
     9421 cttggtcacg gggagcgtcg gcagaccgaa gtcggtcttc agcaccgggc cgaacacccc
     9481 ggccaggacg ccgagcgcga tcagcgtggt gacgatgacc gtccccgcgc cgaacatcct
     9541 gcgcggctgg ccgatggtgc ccagggtccg ggccagcgag atcgactggc ggatcgacgg
     9601 catccgcccg ccctggaggg tggcaccgaa ggcctggaag gcctgccgca tcctctgcag
     9661 gttctctggc ttcgcgaact gctgctgcca gtgctcggcc aggtcctgca ggtcgaagtc
     9721 gtcgctgctc acggcggcag aggatatccc ggtgaacccg gccggggcgg gcgttggttc
     9781 gattgacgct cgaaacgggc cgcgcgtaca ctcgcgattg cccgagggcg ttcttcctgt
     9841 gcgttccgaa tccacagccg gtcccacggt ccgcgcgtcc ggaggagcct tcggcggaag
     9901 gagttgaggg agatcacgga gactatcgag cccggcgcca cggccgcgat gacggccggc
     9961 gccgaggccc cgagcgacga ctacacgccc gaggagctgg cgcgggcgat cgaggagtcc
    10021 ctgaaggact tcaaggacgg cgacctggtg agcggcaagg tggtgcgcat cgaccgcgat
    10081 gaggtgctcc tggacatcgg ctacaagtcc gagggggtca ttcccgccaa ggagctgtcg
    10141 atccgccacg acgtcgaccc cgaccaggtg gtgaaggtcg gcgacgagat cgaggccctg
    10201 gtcctgcaga aggaggacaa ggagggccgg ctgatcctct ccaagaagcg cgcgcagtac
    10261 gagcgcgcgt ggggccggat cgaggagatc atgcgcgagg ggaggacgat caccggcccc
    10321 gtgatcgagg tcgtgaaggg cggcctgatc ctcgacatcg gcctgcgtgg gttcttgccc
    10381 gcctccctcg tcgacctgcg ccgggtgcgc gatctccatc cgttcgtcgg ccaggagctc
    10441 gaggcgaaga tcatcgagct cgaccggaac cggaacaacg tcgtgctgtc gcgccgggcc
    10501 ttcctcgagg agtcgcagag cgagggccga aagaagttcc tggagtcgct gcagaagggc
    10561 gagcgccgca ccggcacggt ctcctcgatc gtcaacttcg gcgccttcgt cgacctcggt
    10621 ggggtggacg gcctggtgca cgtgtccgag ctgtcgtgga agcacgtcga ccatccgtcg
    10681 gaggtcgtgt cggtcggcca ggaggtggag gtcgaggtgc tcgacgtcga gctcgagcgc
    10741 gagcgggtgt cgctgtcgct gaaggccacc caggaggacc cctggcgcga gttcgagcgc
    10801 aaccacaagg ccggcgaggt gatctcgggc caggtgacga agctggtccc gttcggcgcc
    10861 ttcgtccgcg tcggcgaggg gatcgaaggc ctcgtgcaca tctccgagtt gtccgacgcc
    10921 cacgtcgaca tgccggagaa ggtcgtcagc gtcggccagc aggtgcgcgt caaggtcgtc
    10981 gacgtggacg tgtcgcgccg gcgcatctcg ctctcgatgc gccaggtcgg cgagcaggag
    11041 ccggtggcgg agatcgcccc cgagccgtcg ccggtggccg aggtcgccga gcccgaggcg
    11101 gaggccgtcg tcccggccgg cccaccgccc accgtcgagg aggccgccgc cgccgaggcc
    11161 gaggccatgg ccgtcccgga ggacgccccc ccggtcgagc ccgagcccga gcccgcgtcc
    11221 cccgccgcgc cggagccgcc ggccgaggag ccgaccgacg aggacgtctc gctcgagtcc
    11281 atcgtcgagg acctcaagcg ccgcgagggg cgctcctgac ggccggatcg tcgatctctc
    11341 ccgatgcctc gccggtgcgt gcttagcctc gacaaggtct cctcgatcag aggcgggtta
    11401 tgcaccgaac ggatcacctc gctccggccc gacaagatgg cggcggtgtg tgaagcgctc
    11461 cgcgtggcca tggtctgctg agtcgatgct gctcgtgggg ttgaccgggg ggatcgggac
    11521 ggggaagtcg accgtcgctg cccggttggc cgaacggggg gcggtcgtcg tcgacgcgga
    11581 cgtgctgggg cgagaggcgg ttgcgccggg gacggcgggc ttcgagaacg tcgtcaagcg
    11641 gttcggcaag cgactggtgg acccgtcgac cggagggctg gaccgcaacg cactggcggc
    11701 caccgtgttc gccgatgaga tggcgcggcg cgacctcgag gcgatcatcc atcccgaggt
    11761 cgggcggcga ctcgcggaga cggtcgggcg ctaccgggac agcgacgcga tcctcgtctt
    11821 cgacgcgccg ctgatcgtcg agggcgggtt cgcggacatg gtcgacgtgc tggtcgtggt
    11881 gacctcgaag gccgaggaac aggtccgccg gctgatggag ctgcgcggga tggcggagga
    11941 cgacgtccgg gcgcggatcg gggcgcaggc gccgaccgag gagaaggtcg cccgcgccga
    12001 cgtggtgatc gagaacgacg gctccgaggc cgatctcgcc gagagggtcg acgcgctct
//
LOCUS       DASPWO010000492         5889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_582103, whole genome shotgun sequence.
ACCESSION   DASPWO010000492 DASPWO010000000
VERSION     DASPWO010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582103"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1506
                     /locus_tag="VEO00_11235"
     CDS             226..1506
                     /locus_tag="VEO00_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HYU58610.1"
                     /translation="MLTQEEHVEAVALATRGWTISAIARHLDRDRKTIRSYLTGQTAP
                     GVRRRTSDPFGVVEPYVRQRLKDDPHLQLAVLIRELPPLGFAASYQTLTREVRARSLR
                     PHCEACAGAKGRATTDIEHPAGAETQWDWLELPDAPWTSKLIVLTGTLAHSSKTRAWV
                     SEAKDTPHLLQGIDAVLSRLGGLTKAFRIDAMEGAIVPGTRNLCAAFADAARYYGVSV
                     AVCPPRRGNRKGVVEKNNDHIAQSWWRTADVATPEQAQTSLDAFCESVGDARPRGDAT
                     VGALADREPLRPLPSGPYPAVVECSRKVSWAALVSYEGNRYSVPAAFVNAWVIVSVRL
                     GHPVVEIRSGEGEVLAQHRRRPAGAGALARSQEHRAGLERTVLAQFSTARPCRRKEHR
                     PPSDEARAIAARLTRGVQQSFDVDLGSYEELMAT"
     gene            1503..2294
                     /gene="istB"
                     /locus_tag="VEO00_11240"
     CDS             1503..2294
                     /gene="istB"
                     /locus_tag="VEO00_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016695443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HYU58611.1"
                     /translation="MSHDTVYQRLRSHLAYLKLPAAAEALAGKLDHAEKAKPSYTAFT
                     EDLLATEVEATERRRLAGRLRFSHFPVIKRLEDFDFDAQPGLDRRLVEELATLRFVEE
                     KANVLMVGPPGVGKTMLALALGYRAIEAGYRVYYTTAADLAARCHKAAIEGRWATTMR
                     FFAGPQLLICDELGYLPLQSEAASAIFQVVSRRSEQGSVIVTTNRSIASWGEIFDDQM
                     MAAAILDRFLFKATLLAITGDSYRMRAHRERLQQLREGVAATLQR"
     gene            2671..5133
                     /locus_tag="VEO00_11245"
     CDS             2671..5133
                     /locus_tag="VEO00_11245"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HYU58612.1"
                     /translation="MSAAPDAARLTAILESAGSPVRAAEEILDLLGGDVAPFSLLGAL
                     HERSPDRARAIARAARDLAPESETALGLAVAVAEADDDPRRALSLMQGRVDGSSHPSL
                     RVALAAALNREGRFADAAAVVEPLSHELPGLGEAEEELASAVIAAHVRVRTDDGRWTC
                     PCGSGRTYARCCKPGERAVVERFLDRSAFDRFRERISDYSLRPGFAAVRAHAFREWFG
                     DEHPPGPDDLPLGEAMAMIERAWLLLGATGIAGSGDTVLDRFARDRGNSPQDRRRAED
                     WSAWARYGLWRDTGEAAPPGIHLEDLLTGSSHYVAMEPEQREAMGEVFLGMIVPFDGV
                     WRTGATLMPVSVEEAHELRDGLLVRLSEIGAGHGSSPGDALVADLEEVRGFLVPGIPP
                     AGLPPELSLLLSSVAGAALPDLVGLLRARWAREVVLTNTAGEPLEMIEARVAVADLAG
                     TRDRLLARADVWAESDEVLVWEGDRIPPQQLREMHEHLRRQGAEVEAAEEPQRWILGR
                     IRVESDALVVEVNSRARFRRFLGILEALEASPVVVAEETTRVPQPPHAAEALSWLHPS
                     DPLPDPVVPATPDLADAASGSATFVRVRALLALLGDRGRRLTTTGNLQLADGRALAEA
                     MGVEFDDRFGNRVYRRRSSGEVREITETLAMVRAAGLVRVVHGWARPTKRAARFGRDP
                     VADWWSLFESFVRKVRWPERRGDRPWWAGELGDLTTRLLEEIYRAGGEDVAASELAAR
                     AVAVLGVRSRLDQDQRTRLADWIDGDIAYGMMLPLVDLGALALDERPLSRERPAGWTA
                     VEGARTTPLGAWAIHRLITDRP"
     gene            5253..5543
                     /locus_tag="VEO00_11250"
     CDS             5253..5543
                     /locus_tag="VEO00_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYU58613.1"
                     /translation="MTTMTATEARAALPGLLDRVEAGEEVTITRHGRAVAVLVRPDAL
                     RVRKADQALEIAASVRDRSAAARGRTLSSAGALTGARAEELIAEARAGREGR"
     gene            5550..>5889
                     /locus_tag="VEO00_11255"
     CDS             5550..>5889
                     /locus_tag="VEO00_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYU58614.1"
                     /translation="MDAFDADVLIYAAVPGHSLGRRVLALLPDEPVGEQGFPPGIGSV
                     LLLPELLSKPIRDQADDEVTTLTALLSRLDLLPVDQATAELAVALGARYGLKAADAVH
                     LATGVGAGADR"
BASE COUNT          811 a         2078 c         2174 g          826 t
ORIGIN      
        1 tgaagtcctc gcggatccgc gccacgatgg ccgactccgc gtccttcgcc gcaaggcggg
       61 ctgtcccgta cgagtccatg agccgtctcc cttcaacccg caaggtctcg ccagagtacg
      121 gctcatacgg tcctaaagta attccccacc tcctgagcct ccacgaacag ggctcccccg
      181 atgctggcgg tgtcgaagcc accggcagcg ggaggagccc cagggatgct gacacaggag
      241 gagcacgtgg aagccgtcgc actcgccacc aggggatgga ccatctcggc catcgcccga
      301 catcttgacc gcgaccgcaa gacgatccgg tcctacctca cgggccagac cgcccccgga
      361 gtccgtcgcc ggacgagcga cccgttcggg gtcgtcgagc cctacgtgcg ccagcgactg
      421 aaggacgacc cgcacctgca gctcgcggta ctcatccggg agctcccgcc gctcgggttc
      481 gccgcgagct accagacgct cacccgggag gtccgtgccc ggtcgctccg tccccactgc
      541 gaggcgtgcg ccggcgccaa gggccgggcc accaccgaca tcgagcaccc tgctggagcg
      601 gagacgcagt gggactggct ggagctcccc gacgcgccgt ggacgtccaa gctcatcgtc
      661 ctgaccggga cgctcgcgca ctcctcgaag acgagggcat gggtctccga ggccaaggac
      721 accccgcacc tgctccaggg catcgacgcg gtgctgtccc gcctcggcgg gctcaccaag
      781 gcgttccgga tcgacgccat ggagggggcc atcgtccccg gaacccggaa cctgtgcgcg
      841 gcgttcgccg atgccgcccg gtactacggc gtctcggtcg ccgtgtgccc gccccggcgc
      901 ggcaaccgca agggcgtggt ggagaagaac aacgaccaca tcgcccagtc ctggtggcga
      961 acggccgacg tggcgacccc ggagcaggcc cagacgagcc tggacgcgtt ctgcgagtcg
     1021 gtgggggacg cccgtccccg gggcgacgcc accgtggggg cgctcgccga tcgcgagccg
     1081 ctccgtccgc tgccctcggg gccgtacccg gcggtcgtgg agtgctcccg gaaggtgagc
     1141 tgggcggccc tggtgtccta cgaggggaac cgctactcgg tcccggccgc gttcgtgaac
     1201 gcgtgggtca tcgtctcagt gcgtctcggt caccctgtcg tggagatccg ctcgggcgaa
     1261 ggcgaggtgc tggcacaaca ccgccgtcgc ccggccggcg ccggggcgct cgcccgctcc
     1321 caggagcacc gggccgggtt ggagcggacg gtgctggccc agttctccac ggcgaggccc
     1381 tgccggcgca aggagcaccg tccgccgtcg gatgaggcga gggccatcgc cgcccggctc
     1441 acccggggcg tccagcagtc cttcgacgtc gacctcggga gctacgagga gctgatggcg
     1501 acgtgagcca cgacaccgtc taccagcgac tgcgatcgca ccttgcctac ctgaagctcc
     1561 ccgccgcggc ggaggccctg gccggaaagc tcgatcacgc ggagaaggcc aaaccctcct
     1621 acaccgcgtt caccgaggac ctgctggcga ccgaggtcga ggccaccgag cgccggcgcc
     1681 tggcggggcg cctgcggttc tcgcacttcc cggtgatcaa acgactcgag gacttcgact
     1741 tcgacgccca gcccggcctc gaccgtcgcc tcgtcgagga gctcgccacg ctccggttcg
     1801 tggaggagaa ggccaacgtg ctgatggtgg ggcctccggg ggtcggcaag acgatgctcg
     1861 ccctggcgct cgggtaccgg gcgatcgagg ccggctaccg cgtgtactac acgaccgccg
     1921 ccgacctcgc ggcccggtgc cacaaggccg cgatcgaggg ccggtgggcg acgaccatgc
     1981 ggttcttcgc cggcccccag cttctgatct gtgacgagct cggctacctc ccgctgcagt
     2041 ccgaggcggc ctcggcgatc ttccaggtgg tgtcgcggcg ctcggagcag ggctcggtga
     2101 tcgtgacgac caaccgctcg atcgcctcgt ggggcgagat cttcgacgac cagatgatgg
     2161 ccgcggcgat cctcgaccgg ttcctgttca aagcgacgct cctcgccatc accggcgact
     2221 cctaccggat gcgggcccac cgggagcggc ttcagcagct cagggagggg gtggccgcca
     2281 ccctccaacg ctagactcgg ccgggcccag ggtggggaat ttcgatgagc gcccctgggg
     2341 aatttcggtg agcgccgtca agcccacaat ctggggaacg gattcctggc gtttgtgcag
     2401 gtcagagtgc tgagtcggcg cgggcccggt gtcgatcccc gcacggagtc cggcgctggg
     2461 cggtaccatc accgggtctt ccccgtggca cggagggacc acgtggggaa gcgtgagagg
     2521 gaacggcggc ggcgcgagcg ccgaatggcc ctgctgcacg gcggcgcagg gaagggtggc
     2581 gactcacggc gcgacgggag atcggtcggc cgcagggtgc acggcgcgtc tcatcatccc
     2641 gccgaagcct tcgaccccgg agccctgcgc gtgagcgccg cgccggacgc cgcccggcta
     2701 acggcgatcc tcgagtccgc cggctcgccg gttcgggccg cggaggagat cctggacctc
     2761 ctcggcggcg acgtcgcccc cttctcgctc ctcggggccc tccacgaacg atccccggac
     2821 cgggcgcgag cgatcgccag ggccgctcgc gacctggcgc cggagtccga gaccgccctc
     2881 ggcctggcgg tggccgttgc cgaggccgac gacgatcccc gccgcgcgct gtccctgatg
     2941 caggggaggg tcgacgggtc atctcacccc tcgctccgcg tggctctggc ggccgccctc
     3001 aaccgcgagg gccggttcgc ggacgctgcc gccgtggtcg aaccactcag ccatgagctg
     3061 ccggggctcg gggaggccga ggaggagctc gcgtctgcgg tgatcgcggc ccacgtccgc
     3121 gtcaggaccg acgacgggag gtggacctgc ccgtgcggct cggggcggac gtacgcgagg
     3181 tgctgcaagc cgggcgagcg ggcggtcgtg gaacgattcc tcgaccgttc ggcgttcgac
     3241 cggttccgcg agcgaatctc tgattactcc cttcgccccg gtttcgcggc ggtgcgggcg
     3301 cacgcgttcc gggaatggtt cggggacgaa caccccccgg gcccggatga cctgccgctg
     3361 ggggaggcca tggccatgat cgaacgggcc tggctcctcc tgggcgcgac cgggatcgcc
     3421 ggatcgggcg atacggtgct cgaccgtttc gcccgcgacc gggggaactc tccccaggac
     3481 cgccggcgcg ccgaggactg gtcggcgtgg gcccggtacg ggctgtggcg ggacaccggc
     3541 gaggccgcac cgcccggcat ccacctcgag gacctgctga ccggctcgag ccactacgtc
     3601 gcgatggagc cggagcagcg cgaggcgatg ggcgaggtgt tcctggggat gatcgtcccc
     3661 ttcgacggcg tgtggcggac gggtgcgacc ctcatgccgg tgtcggtgga ggaggcccac
     3721 gagctccgcg acgggcttct cgttcggctg tcggagatcg gggccggcca cggatcgtcc
     3781 cccggcgatg cgctcgtggc cgacctcgag gaggtgcgag gcttcctggt gcccgggatc
     3841 ccccccgccg ggctcccccc ggagctctcg ctcctgttgt cctcggtggc cggggcagcc
     3901 ctccccgacc tggtcggtct cctgcgggca cggtgggccc gggaggtggt gctgacgaac
     3961 acggcgggag agccgctcga gatgatcgag gcccgggtcg cggtggcgga tctcgccggg
     4021 acgcgggacc gcctcctggc gagggccgac gtgtgggccg agtcggacga ggtgctcgta
     4081 tgggagggcg accggatccc cccgcagcag ctccgcgaga tgcacgagca ccttcgccgg
     4141 cagggggccg aggtcgaggc cgcggaggaa ccccagcgct ggatcctcgg gcggatccgg
     4201 gtggagtccg acgccctggt cgtcgaggtc aactcgcgcg cccgcttccg gcggttcctg
     4261 gggatcctgg aggcgctgga ggcgagcccc gtggtcgtcg ccgaggagac cacgcgggtg
     4321 ccgcagcccc ctcacgccgc cgaggcgctg tcgtggctgc atccgtcgga cccgttgccg
     4381 gaccctgtgg tgccggcgac cccggacctc gccgacgcgg cgagcggctc cgcgacgttc
     4441 gtccgggttc gggcgctcct cgctctcctc ggcgacaggg ggcggaggct cacgacgacg
     4501 gggaacctcc aactggccga cggtcgcgca ctggccgagg cgatgggcgt cgagttcgac
     4561 gaccggttcg ggaaccgcgt gtaccgccgg cggagctccg gcgaggtcag ggagatcacg
     4621 gagacgctgg cgatggttcg cgccgccggt ctcgtccggg tggtccacgg gtgggcccgc
     4681 ccgacgaagc gggcggcgag gttcggccgg gaccccgtcg ccgactggtg gagtctgttc
     4741 gagtcgttcg tccgcaaggt gcgctggccc gagcgccgcg gggaccggcc ctggtgggcc
     4801 ggcgagctgg gagacctcac cacgcgcctg ctcgaggaga tctaccgggc cggcggggag
     4861 gacgtggcgg cgagcgagct ggccgcccgg gccgttgccg tgctgggcgt ccgctcccgc
     4921 ctcgaccagg accagcgaac caggctggcg gactggatcg acggcgacat cgcctacggg
     4981 atgatgcttc ccctggtgga tctcggcgcc ctggcgctcg acgaacgccc gctttcgcga
     5041 gagcggccgg cgggatggac cgccgtcgag ggagcgcgca ccacgccgct cggcgcgtgg
     5101 gccatccacc ggctgatcac cgaccggccc tgacctgccg gcgatgacgt gacggggggt
     5161 tcaggtccgg attctcgcgg agggagcgac gcggctctcg cgaaggtggc cttcgtgtac
     5221 tgcaccgtac atcgctgtac aatcggatcg ccatgacgac gatgaccgca acggaagcga
     5281 gggcagccct cccgggcctg ctcgaccggg tcgaggcggg tgaggaagtg accatcaccc
     5341 gccatggacg agcggtcgcc gtgctcgttc gtcccgacgc cctccgggtt cgaaaggcgg
     5401 atcaggcgct ggagatcgcc gcctcggttc gcgaccggag cgccgcggcc cggggaagga
     5461 cgctgtcctc ggcaggtgcg ctcacgggcg cgcgcgccga ggagctgatc gccgaggcac
     5521 gagcgggacg cgagggccgc tgagcgcgaa tggatgcgtt cgacgcggac gtgttgatct
     5581 acgcggccgt gccgggacac tcgctcggcc gtcgcgtcct cgccctgctt ccggacgagc
     5641 cggtgggcga gcaggggttc cctcccggta tcggctcggt gctgctgctc ccagagctct
     5701 tgagcaagcc gatccgggac caggcagacg atgaggtgac aacgctcacg gcgctgctga
     5761 gcaggttgga tctcctgccg gtcgaccagg cgaccgccga actcgccgtc gccctcgggg
     5821 cccggtacgg actgaaggcc gcggatgccg tgcacctcgc gacaggggtc ggcgccggcg
     5881 ccgaccggt
//
LOCUS       DASPWO010000493         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_152056, whole genome shotgun sequence.
ACCESSION   DASPWO010000493 DASPWO010000000
VERSION     DASPWO010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152056"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VEO00_11260"
     CDS             <1..306
                     /locus_tag="VEO00_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58615.1"
                     /translation="HILGTASGNTLVAGPSGPRCMFELFNDPPFEVASSNRHPAGVQL
                     TCALEGKIAAGGIERKARKLLKLLIPVAVVLLVVVVALAVWFRRRRSRAPSGEAAGS"
     gene            303..446
                     /locus_tag="VEO00_11265"
                     /pseudo
     CDS             303..446
                     /locus_tag="VEO00_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007529570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
     gene            complement(469..1167)
                     /locus_tag="VEO00_11270"
     CDS             complement(469..1167)
                     /locus_tag="VEO00_11270"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYU58616.1"
                     /translation="MGGKAGHDAPRIRDVPGPRRNQSVIGPLFQRVFMWPYRAALVGL
                     YRVGFRPWQLTILSLLTNAAVGWLLVDGYRFVPGMLLIPAGLFDVFDGGVARLRGEES
                     RLGAFLDSTLDRVSDGILFGCLFWSEAGQGHHTTSILALSTLGISLVVSQIRAEGEAL
                     GVKLSEGVFQRLERYVALMVGLTAPGALLPVLAILTGLGGITVAQRVWSAWRGLAKPP
                     PEPAAPVPDQSRAG"
     gene            complement(1442..1984)
                     /gene="greA"
                     /locus_tag="VEO00_11275"
     CDS             complement(1442..1984)
                     /gene="greA"
                     /locus_tag="VEO00_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HYU58617.1"
                     /translation="MVELPEELASRPPRPAGHEPTATLTLDAYRRLKDELDGLRSEGR
                     THISERLKAAREHGDIRENAEYDAAKNEQGLMEARIRQLERMLRDPDIVEAPAHSDQV
                     GPGMLVTVLPLDDDDPEEETYLLADSAEERAQGGVRTITTTSPLGSAMVGTAKGDEVV
                     SQAPGGNFRYRVVEFRPHTA"
     gene            complement(2309..>2782)
                     /locus_tag="VEO00_11280"
     CDS             complement(2309..>2782)
                     /locus_tag="VEO00_11280"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYU58618.1"
                     /translation="SRGNAIGIADYSAPAVPEDRPAPTFTLPGLAGRGAVSLDDLSGH
                     PVVLSFWASWCPPCREEAPFLKSAWSTYRSEGVRFLGVDHRDSRPAGLSFARRMAIPF
                     PSVYDAGGELAGRYGLFGVPTTFVIRADGRIAYRITGRVDRTNLRTALKSLVSSG"
BASE COUNT          425 a          976 c          983 g          398 t
ORIGIN      
        1 cacatcctcg gaacggcctc cgggaacacg ctggtggccg gcccgagcgg gccgaggtgc
       61 atgttcgagc tgttcaacga cccgccgttc gaggtcgcgt cgtcgaacag gcacccggcg
      121 ggggtgcagc tgacgtgtgc cctggagggc aagatcgccg ccggcggcat cgaacggaag
      181 gcccgcaagc tgctgaagtt gctgatcccg gttgcggtgg tgttgctcgt cgttgtggtg
      241 gcgctggcgg tgtggtttcg ccgccgccgg tcgcgggcgc catcggggga ggcagcggga
      301 tcgtgacgcg gtccaagccg cagatcgaac ggctccgtcg gatctgcctg tcgttccccg
      361 aggcgacgga gcgcctgagc cacggcgagc cgacctggtt cgtccggggc aagtccgtgt
      421 tcgtcacgac cgccgaccat caccacgcac catcgcgccc cgcgctggtc agccggctcg
      481 actctggtcg ggaaccggcg ccgcgggctc cggcggaggc ttcgccaggc cgcgccaggc
      541 cgaccagacg cgctgggcca ccgtgatgcc gccgaggccg gtgaggatag ccaggaccgg
      601 aagcagggcc cccggggccg tcagtccgac catcagcgcg acgtagcgct ccaggcgctg
      661 gaacacccct tctgagagct tcacgccgag ggcctcgccc tccgcccgga tctggctcac
      721 caccagcgag atgccgagcg tggagagcgc caggatcgac gtggtgtggt ggccctgccc
      781 ggcctccgac cagaacaggc agccgaacag gatgccgtcg gagacgcggt cgagggtcga
      841 gtcgaggaac gcgccgagcc ggctctcctc gccccgcaga cgggccacgc cgccgtcgaa
      901 cacgtcgaac agccccgctg ggatcagcag catccccggc acgaaccggt acccgtccac
      961 gagcagccag ccaacggcgg cgttcgtcag cagcgacagg atcgtgagct gccacggccg
     1021 gaaccccacc cggtacagcc ccaccaacgc cgcgcgatac ggccacatga acacgcgctg
     1081 gaacagcggc ccgatgacgc tctggttccg ccgcggcccc ggcacgtccc ggatccgcgg
     1141 cgcgtcgtgc cccgcctttc cacccatccc atgatcaggc tagccgtgcc ccacccggca
     1201 tgcgagcgcc tactcacagc gatggggccc cggcagggcg tggaccttcc cgtcgtaggc
     1261 cgtaacagct cgctcgattt gacggtccac gcccgcgtcc aggcggtgct ggaggacagc
     1321 tccggcaacg cggtccggat gaccacggct gcccacgacc ccaccccgcc atgatgcgcc
     1381 agctccggta ccgcgaccgc tggtcgtacc gctggcgtcg agagcagccg cgaccgcggg
     1441 cctacgcggt gtgggggcgg aactcgacga cgcggtagcg gaagttgccg ccgggggcct
     1501 gggagacgac ctcgtcgccc ttggcggtgc ccaccatggc ggagccaagt ggcgacgtgg
     1561 tggtgatggt gcggacgccg ccctgagcgc gctcctcggc ggagtcggcc aggaggtagg
     1621 tctcctcctc ggggtcgtcg tcgtcgagcg ggagcaccgt caccagcatc cctgggccga
     1681 cctggtcgga gtgggcgggg gcctcgacga tgtcggggtc gcggagcatc cgctcgagct
     1741 ggcggatgcg cgcctccatg agtccctgtt cgttcttcgc ggcgtcgtac tcggcgttct
     1801 cgcggatgtc gccgtgctcg cgggcggcct tgagccgctc ggagatgtgg gtgcgaccct
     1861 cggacctcag gccgtcgagt tcgtccttca gccgccggta ggcgtcgagc gtcagcgtcg
     1921 cggtcggctc gtgtcctgcg ggacgcggtg ggcgggacgc cagctcctct gggagttcca
     1981 ccatggacgg aactgtaaca gcgggtcgtg gtgccctcgt cgttgggttg tcccgcggcg
     2041 ctgccccctc ggctgctcgc ggtcgtcccg cagcgccgac cagcagaggg cgccgaaagc
     2101 gccgacgagc cccaccgtca gcgaggcctg gatccagtcg cccacggaag ccggatcgat
     2161 catcttgtgc ccctttccgt cagatcggtt cttcgacacg ggagatggaa gcagccgaag
     2221 cttggaagtc cctggagacg ggcgaagaga caggtgagac caaggccaag cttgatcccg
     2281 gtgacggaga aacccggacc tggtcgcgtc agccggagct gacgagactc ttgagggcgg
     2341 ttctcagatt cgttcggtcg accctgcccg tgatcctgta ggcgatccgc ccatctgccc
     2401 ggatcacgaa cgtcgtgggg acgccgaaca atccatatcg gccggcgagc tcgccgccgg
     2461 cgtcgtagac gctcggaaag gggatcgcca tgcgtcgcgc gaaggacagg ccggcaggcc
     2521 gagagtcccg gtgatccacc cccaggaacc ggactccttc cgaccgatac gtgctccacg
     2581 ccgacttcag gaagggcgcc tcctcgcggc acggggggca ccaggaggcc cagaagctca
     2641 gaaccaccgg atgcccggag aggtcgtcga gcgagacggc tccccttccc gcgagtcccg
     2701 gcagggtgaa cgtcggggcc gggcgatcct ccggcaccgc cggcgcggag tagtccgcga
     2761 tgccgatcgc gttcccccgg ga
//
LOCUS       DASPWO010000494         4210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1171433, whole genome shotgun sequence.
ACCESSION   DASPWO010000494 DASPWO010000000
VERSION     DASPWO010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171433"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..997
                     /locus_tag="VEO00_11285"
     CDS             260..997
                     /locus_tag="VEO00_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58619.1"
                     /translation="MSRLFRRFLGKGRAVSFVSLLSYLHHPLITPREEPSQRASLDPL
                     EVAEHVRRCREALEATSAYRSEQLLRIDRVEEPVIIWTIEHVVPDRYHVVQSAWRDPD
                     RPTGELTDQLFDEWLIVGSDHFLKIMGWMRVDQSSRMSEQFLLRKYLELLGRPDTRLV
                     GVSSVEGSSYLQLECPSLPASSARIYEVPDAAGQTEMWVDLSTDMLARADQVWSQSDN
                     ERRNEFHRVEVFFDQGEAISIEPPEGV"
     gene            complement(1206..1278)
                     /locus_tag="VEO00_11290"
     tRNA            complement(1206..1278)
                     /locus_tag="VEO00_11290"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1243..1245),aa:Ala,seq:ggc)
     gene            1388..1978
                     /locus_tag="VEO00_11295"
     CDS             1388..1978
                     /locus_tag="VEO00_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020138421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HYU58620.1"
                     /translation="MRKVIVSEFLTLDGVMQAPGDPDEDRSGGFEAGGWQLAYFDDVF
                     GKAVMDTFDKIDGFLFGRRTYEIMAAYWPTATEPEPLAGMMNRLAKHVVSTTLEEPLA
                     WENSRLVGDDVPTAIAELKGRPGKDLAVLGSGRLVQTLMKHDLVDEYRLMIHPLVLGS
                     GRRLFGEGSPPSRLRLVDSATSGTGILILTYRPEKG"
     gene            2022..2330
                     /locus_tag="VEO00_11300"
     CDS             2022..2330
                     /locus_tag="VEO00_11300"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HYU58621.1"
                     /translation="MAIFVYLRFSLDEGFDRQDFEGDMRAMAELAREQPGHRWSEMGR
                     SMADPSVYVVVSEWDDVDQVRAWEHQPVHVQVQQKWESRFREPLLHRRFVPWERPPET
                     "
     gene            complement(2369..2752)
                     /gene="rsfS"
                     /locus_tag="VEO00_11305"
     CDS             complement(2369..2752)
                     /gene="rsfS"
                     /locus_tag="VEO00_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HYU58622.1"
                     /translation="MTAAPVVSSRALAVEAARAASAKQARDVVVMEMRDLLVITDYFV
                     VAGGTSDRQVRTVAEEIERALRERGVKPVRREGEREGRWVLLDYVDLVVHVFLDEERE
                     FYDLERLWADAPRVGWEHAGAVSNG"
     gene            complement(2749..3834)
                     /locus_tag="VEO00_11310"
     CDS             complement(2749..3834)
                     /locus_tag="VEO00_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HYU58623.1"
                     /translation="MTGERVRRATLRRRRRRVRLLSAMAVLAVAGSVAGYVLLAGDGR
                     PGPEEAPPSGAGADAQVVTAFGYDGRRDEVAVLASAGGKDSLVMVVPANLDVSTPGHG
                     TGPVGRAVPGGAETVGTTVANLLGIEVDHRGVLASDGLAGFVEAIGGLAVRLGAAANL
                     GERNAGPGEVELTGAEAAFYLDRSPGDPLERSLRFEEVVAGLVRAAGDGAALQDAAAD
                     ADGGFGAAVGSVGDDPRIEEVPVVDPTAAATAIDREAADRLVRELFVRPTGTAATVVV
                     LNGNGVPGIGQAVAERIVPEGFRIVVSTNARAFDVETTRIEVGTEGKRELGERIRSLL
                     GVGEVFVGEEATGLADITIVVGRDFRP"
     gene            complement(3831..>4210)
                     /locus_tag="VEO00_11315"
     CDS             complement(3831..>4210)
                     /locus_tag="VEO00_11315"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HYU58624.1"
                     /translation="RTEIDRGGPTYTRDTLLELLDHVGPSAELFFITGADAMLEIFHW
                     KDPEDVLSLAHFIAATRPGYDIPRFEREAPTSHPNVSVMDIPALAISSTDIRRRVKEG
                     RPIRYLVPAGVEAYIQKAGLYRG"
BASE COUNT          677 a         1476 c         1425 g          632 t
ORIGIN      
        1 gggacacatc gagtgctgga acctcgaccc gcaggcctat gagtcgaaga tgatctcctt
       61 cctgaacgac gcgacctcct gagccgacga cgacgcgccg cgaagccacc cggggactcg
      121 cggatatcgc ggaacacgac atgcgaggac ctgaggggat gctattcgaa ccgcctcaat
      181 gagctgaagc agctggtcgc cccagcgcgc cagaaggggg acaatgagat gctcgggttc
      241 ggggatactg gggtcgagaa tgagtaggct gtttcgacgc tttctgggca aaggccgagc
      301 ggtgtcgttc gtgtccctcc tcagctatct gcaccacccc ctgatcactc cccgagagga
      361 accctcccag agggcatcac tcgatcccct cgaggtcgct gagcatgtgc gacggtgccg
      421 ggaagcgctc gaggcaacct cggcgtaccg atcggaacaa ctcctcagga tcgaccgggt
      481 ggaagagcct gtgatcatct ggacgatcga gcacgtcgtc ccagaccgat accacgttgt
      541 ccaaagcgcc tggcgagacc cggatcgccc gacaggagag ttaactgatc aactctttga
      601 cgagtggctc atcgtgggtt ctgaccactt cctgaagata atggggtgga tgcgggttga
      661 ccaatcgagt cggatgagcg agcagttctt gcttcggaag tacctcgaac tcctgggtcg
      721 tccggatact cggttggttg gagtctcatc ggtcgaggga agcagttacc tacagctgga
      781 gtgtccgtcg ttaccagctt cgtctgctcg tatctacgaa gtgccggacg cggcagggca
      841 gaccgagatg tgggtggatc tctccacgga catgctcgca agagcggatc aggtctggtc
      901 gcaatcagat aacgagcgta ggaacgaatt ccatcgagtg gaggtcttct tcgatcaggg
      961 ggaggcgatc tcgatcgaac cacccgaggg ggtgtaacca cttcaccggc tagccgttcc
     1021 gagttcgcgg tcggcgtaat tgcgggtctg accgccaccc aggaggaggg cagctgcgcg
     1081 gccatcggaa gggtcgccgc acagtggacc tgtgccggcc ggggagtcca ccgaaaccgg
     1141 catcgccacg aagataaccc actattcggg aacagggccc cgcgtttgcc ctggtcaggg
     1201 ctttgtggac ctgaggggag tcgaacccct gacctcttgc ctgccgagca agcgctctac
     1261 caactgagct acaggcccta gatgggccga ttctatcagc cgttgatgtc atcccccgga
     1321 gacccgccga cgcgttggag gcggtcgggc gtgggtacgg ataccgcgac gaggaaggag
     1381 gagcgggatg cgcaaggtga tcgtgagcga gttcctgacg ctggacgggg tgatgcaggc
     1441 ccccggggac ccggacgagg accgcagcgg cgggttcgag gccggcgggt ggcagctcgc
     1501 ctacttcgac gacgtcttcg gcaaggccgt catggacacg ttcgacaaga tcgacgggtt
     1561 cctgttcggg cggcggacgt acgagatcat ggccgcctac tggccgacgg cgacggagcc
     1621 cgagccgctc gccgggatga tgaaccgcct ggccaagcac gtcgtctcga cgacgctcga
     1681 ggaacccctg gcgtgggaga actcgcgcct ggtcggcgac gacgtcccca cggcgatcgc
     1741 cgagctgaag ggccggcccg gcaaggacct tgccgtcctc ggcagcggcc ggctcgtgca
     1801 gacgctgatg aagcacgacc tggtcgacga gtaccgcctg atgatccacc cgctcgtcct
     1861 ggggagcggg cggcgcctgt tcggtgaggg cagccccccg agccgccttc gcctggtcga
     1921 cagcgcgacg agcgggacgg gcatcctgat cctcacctac cggccggaga aaggctgagt
     1981 cccgaagcgc ggccggcggc ccccggcgta gggtcggcgc tgtggcgatc ttcgtctatc
     2041 tccgcttctc cctcgacgag ggcttcgatc gacaggactt cgagggcgac atgcgggcga
     2101 tggccgagct ggcccgcgaa cagcccggcc accggtggtc agagatggga cggtcgatgg
     2161 cggatccgtc ggtctacgtc gtggtatcgg agtgggacga cgtcgaccag gttcgcgcct
     2221 gggagcacca gccggtccac gtccaggtcc agcagaagtg ggagtcccga ttccgcgagc
     2281 cgctcctgca ccgccggttc gttccctggg agcgcccccc ggagacgtga ccgggagcca
     2341 tcgatctgag gcttcggcgg gctcggcctc acccgttgga gacggcgccg gcgtgctccc
     2401 atccgacgcg cggggcgtcg gcccacaggc gttcgaggtc gtagaactcg cgctcctcgt
     2461 cgaggaagac gtggaccacc aggtccacgt agtcgagcag gacccagcgg ccttcgcgct
     2521 cgccctcgcg gcggaccggt ttgacgccgc gctcgcgcag ggcgcgttcg atctcctcgg
     2581 ccaccgtgcg gacctggcgg tccgaggtcc cgccggcgac cacgaagtag tcggtgatca
     2641 ccaggaggtc gcgcatctcc atcacgacca cgtcccgggc ctgcttcgcc gaggccgccc
     2701 gggcggcctc caccgccagc gctcgcgacg acaccaccgg cgccgccgtc atggccggaa
     2761 gtcccttccc acgacgatcg tgatgtcggc cagccccgtc gcttcctcgc ccacgaacac
     2821 ctcccccact ccgaggagcg agcggatccg ctcgcccagc tctcgcttcc cctccgtacc
     2881 cacttcgatc cgggtggtct ccacgtcgaa cgcccgcgcg ttcgtcgaga ccacgatgcg
     2941 gaacccctcc ggcacgatcc gctcggccac cgcctggccg atcccgggca cgccgttgcc
     3001 gttcaagacc accaccgtcg ccgccgtgcc ggtcgggcga acgaacagct ctcgcaccag
     3061 gcggtcggcc gcctcccggt cgatggcggt ggccgcggcg gtcgggtcga ccaccggaac
     3121 ctcctcgatc cgcgggtcgt cgcccaccga gccgaccgcc gcgccgaacc ctccgtcggc
     3181 gtcggccgcc gcatcctgga gcgcggcacc gtccccggca gcccgcacca ggccggccac
     3241 cacctcctcg aaccgcagcg agcgctccag cgggtcgccc ggcgaacggt ccaggtagaa
     3301 cgccgcctcc gcgccggtca gctcgacctc acccgggccg gcgttccgct cccccaggtt
     3361 cgccgccgcg ccgaggcgca ccgccagccc cccgatcgcc tcgacgaagc cggccaggcc
     3421 gtcggacgcc agcacgccgc ggtggtcgac ctcgatcccg agcaggttcg ccaccgtggt
     3481 cccgaccgtc tcggcgccgc cgggcacggc gcgcccgacc gggccggtgc cgtggccggg
     3541 cgtgctgacg tcgaggttcg ctggcacgac catcaccagc gagtcctttc cccctgccga
     3601 cgcgagcacc gccacctcgt cacggcgtcc gtcgtagccg aacgcggtca cgacctgggc
     3661 gtcggccccg gcgccgctgg gcggggcctc ctccggaccc ggtcgcccgt cgccggccag
     3721 caggacgtag ccggccacgg atccggccac ggccaggacg gccatggccg agagcagccg
     3781 gacccggcgg cggcgacggc ggagggtggc gcgccggacc cgctcgccgg tcacccgcgg
     3841 tagagccccg ccttctggat gtacgcctcg acaccggccg gcaccaggta gcggatcggc
     3901 cggccctcct tcacccggcg gcggatgtcg gtcgacgaga tcgcgagcgc cgggatgtcc
     3961 atcaccgaca cgttcgggtg ggaggtcggc gcctcccgct cgaaccgcgg gatgtcgtag
     4021 ccgggccggg tcgccgcgat gaagtgcgcc agcgacagca cgtcctcggg gtccttccag
     4081 tggaagatct cgagcatcgc gtcggcgccg gtgatgaaga acagctccgc gctcgggccg
     4141 acatgatcga gcagctcgag cagcgtgtcc cgcgtgtacg tcgggccgcc gcggtcgatc
     4201 tcggtcctcg
//
LOCUS       DASPWO010000495         4002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1091238, whole genome shotgun sequence.
ACCESSION   DASPWO010000495 DASPWO010000000
VERSION     DASPWO010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091238"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VEO00_11320"
     CDS             <1..1027
                     /locus_tag="VEO00_11320"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58625.1"
                     /translation="SAGVPAGAVRSTAADAGEPPEELADQGPALSYYEAGAAPTATWL
                     RSPPASYLPAPAPQRVPSPIWRPWRFAAGAALGLATAVKWSAVTALAGAAILSFFWEI
                     GRRRRAFRRSPIGEAMLQESLGLVLSFLFVPAVAYLATYVPWLHEHGWSLRSLVGHHR
                     DMWDFHAGLQARKPNGELTHPYQSEAWSWPFLRRPVAYFYEGTDTPPTSAEILGIGNP
                     VIFWGSLFAIPFTVFAWARRRDWIAGVIAIGFLSQYLPWLAVSRPAFLFYMTPITPFM
                     VLAGAYALRHLAHARLSTGARAFAPAAGFLVVLAVGVFAFFVPVLVGHVISRGAWNLR
                     MWFPSWV"
     gene            1032..1391
                     /locus_tag="VEO00_11325"
     CDS             1032..1391
                     /locus_tag="VEO00_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HYU58626.1"
                     /translation="MDARKRRRRFDELAGEALDRLPRWVQERMENVEVLVEELPPADA
                     PNLLGLYEGVPLTNRGWGYGVGGTLPDRITLFRATLEAGDPTADELRDRIRHTIVHEV
                     AHFFGISDDRLREIDRY"
     gene            complement(1456..3123)
                     /locus_tag="VEO00_11330"
     CDS             complement(1456..3123)
                     /locus_tag="VEO00_11330"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYU58627.1"
                     /translation="MRRLQTILACALLFASILAASGPGAEAAKPQSLMAGRRSVDAAL
                     LAQARANANGASTGVDNPAPSEIECATETIGPSDHNLDCDHPWLPKNEQDIEVDPNDP
                     LHMVVSANDYESCCDQYDTTFDGGDHWTTGDMSKEDLTRIGSDPVTVFDPVSHNVIHS
                     SLNFTFNQDGADDGDLVVSLSHNGGLTWPDVVVVGNGLGDDNDPSQLFWDKEWLVTDT
                     NPLSPYYGRTYMTSTLFFSEFGAYKKSPIYLSYSDDGGETWTSPKVISGKSNALCTFQ
                     VAGASGVCDQDQFSVPTVGPDGTVYVAFENEQNQSIWEGPGEVDNQYLLVRSFDGGAT
                     WTKPRFVVGMEDGRSDYPINVDGRQTVSKLQFRLNSAGNIVADPDNNGQLYVAFSDNR
                     NGTKQETNVDVFLMRSTNGGQTWSGPHLVSDAPGDDLDLSDQWFPWVDVSSQGTVGVV
                     YNDRTGVGTYEVTFAETAPWGGGSPSWTYTDVTSAASNPRNSLVFQAGVQGCFKCATF
                     IGDYINVDYGSDEAANLAWTDMRQFVVLGSNPLDQGYTQHSFFRRIP"
     gene            complement(3158..>4002)
                     /locus_tag="VEO00_11335"
     CDS             complement(3158..>4002)
                     /locus_tag="VEO00_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58628.1"
                     /translation="LGFLRPLGRLSPWVPRLLAIHAGVSLLAQSARGTYLAPSMRLAD
                     NPVGIALAILQGVLGVWLISGVRIRPAAGVLVLAGPLGMLVYGPVAILERADLLGIAL
                     FLAVLPPGRDRYGAAEPERPRVLWSVFYLRMLVGLSLLVVAFTEKLIDPHLALEFLDR
                     YPVLNVARSLGLPVSDLMFVRIAGGIEVLFGLLLISGAAPQIAVVLTGIPFNATLFFF
                     GASELIGHLPVYGAMLALLVYGSEPALAPLVPVLDPWRAERSARRMGGMAHSGPSPGT
                     APLI"
BASE COUNT          568 a         1410 c         1411 g          613 t
ORIGIN      
        1 ttcggccggc gtccccgccg gcgccgtgcg gagcacggcg gccgacgcgg gggaaccgcc
       61 cgaggaactg gccgatcagg gaccggcgct ctcgtactac gaggcgggcg cggcgccgac
      121 ggcgacctgg ctgcggtccc cgccggcctc ctacctgccg gcgcccgccc cgcagcgggt
      181 gccctcgccg atctggcggc cgtggcgctt cgcggccggc gcggcgctcg gcctggccac
      241 cgcggtgaag tggtcggcgg tgacggcgct cgccggcgcc gcgatcctgt ccttcttctg
      301 ggagatcggc cggcggcggc gggcgttccg gcggtcgccg atcggcgaag ccatgctcca
      361 ggagtcgctc ggcctggtcc tgtcgttcct gttcgtgccg gcggtcgcct acctggcgac
      421 ctacgtgccc tggctgcacg agcacggctg gagcctgcgg tcgcttgtcg gccaccaccg
      481 ggacatgtgg gacttccacg ccgggctcca ggcgcgcaag cccaacgggg agctgaccca
      541 tccctaccag tcggaggcgt ggtcctggcc cttcctgcgc cggccggtgg cctacttcta
      601 cgaggggacc gacacccccc cgacatcggc cgagatcctc ggcatcggga accctgtgat
      661 cttctggggt tcgctcttcg ccatcccctt cacagtgttc gcgtgggcgc gccggcgcga
      721 ctggatcgcc ggcgtgatcg ccatcgggtt cctgtcccag tacctgccct ggctggccgt
      781 gagccggccc gccttcctct tctacatgac cccgatcacg ccgttcatgg tcctggccgg
      841 ggcctacgcc ctgcgccacc tggcgcatgc gcggctgtcg accggagcgc gcgcgttcgc
      901 cccggcggcg gggttcctgg tggtgctcgc cgtcggcgtg ttcgccttct tcgtcccggt
      961 cctggtcggc cacgtcatct cgcgtggggc ctggaacctg cggatgtggt tcccgagctg
     1021 ggtgtgacgg gatggacgcg cggaagcggc ggcgacggtt cgacgagctg gcgggagagg
     1081 cactggaccg gctcccgcgg tgggtgcagg agcgcatgga gaacgtcgag gtcctcgtcg
     1141 aggagctgcc gcccgccgac gcgccgaacc tgctcgggct ctacgagggg gtccccctca
     1201 ccaaccgggg ctggggctac ggcgtcgggg ggacgctccc cgaccgcatc acgctgttcc
     1261 gcgccaccct cgaggccggc gacccgaccg ccgacgagct gcgcgaccgc atccggcaca
     1321 cgatcgtcca cgaggtggcg catttcttcg gcatctccga cgaccgcctg cgcgagatcg
     1381 accggtactg accgccggac cacatggtgg gggggcgggt cgccccgccc cctcgggccg
     1441 tgccgtcgct gcccgctacg ggatacgccg gaagaacgag tgctgggtgt atccctggtc
     1501 gagcgggttc gagccgagga cgacgaactg gcgcatgtcg gtccacgcca ggttcgccgc
     1561 ttcgtccgag ccgtagtcga cgttgatgta gtcgccgatg aacgtggcgc acttgaagca
     1621 cccctggacg cccgcctgga agaccaggga gttcctgggg ttcgacgccg cgctggtgac
     1681 gtccgtgtac gtccacgacg gcgagccgcc gccccacggc gccgtctcgg cgaaggtcac
     1741 ctcgtacgtc ccgacaccgg tacggtcgtt gtagacgacg ccgacggtgc cctgcgagct
     1801 gacgtccacc cacgggaacc actggtccga caggtcgagg tcgtcgccgg gggcgtccga
     1861 caccaggtgc ggaccgctcc acgtctggcc gccgttcgtc gaccgcatca ggaacacgtc
     1921 gacgttcgtc tcctgcttgg tcccgttgcg gttgtcggag aaggccacgt acagctggcc
     1981 gttgttgtcc gggtcggcga cgatgttccc tgccgagttc agccggaact gcagcttcga
     2041 caccgtctga cgcccgtcca cgttgatcgg gtagtcgctg cggccgtcct ccatcccgac
     2101 cacgaacctc ggcttggtcc acgtcgcgcc cccgtcgaac gagcgcacca gcaggtactg
     2161 gttgtcgacc tcgcccgggc cctcccagat cgactggttc tgctcgttct cgaaggcgac
     2221 gtagaccgtc ccgtcgggac cgaccgtcgg caccgagaac tggtcctggt cgcagacgcc
     2281 ggaggcaccg gcaacctgga acgtgcagag cgcgttgctc ttgccggaga tcaccttcgg
     2341 gctggtccag gtctcgccgc cgtcgtcgct gtacgagagg tagatcggcg acttcttgta
     2401 cgccccgaac tcggagaaga acagggtgga ggtcatgtag gtccggccgt agtacggcga
     2461 gagtgggttg gtgtcggtga cgagccattc cttgtcccag aacagctggg aggggtcgtt
     2521 gtcgtcgccg aggccgttgc cgaccacgac cacgtcgggc caggtcaggc caccgttgtg
     2581 cgagagcgac acgacgaggt caccatcgtc ggccccgtcc tggttgaacg tgaagttcag
     2641 cgaggagtgg atgacgttgt gggacacggg gtcgaacacc gtcaccgggt cgctgccgat
     2701 ccgggtgagg tcctccttgg acatgtcgcc ggtggtccag tgatcgccgc cgtcgaacgt
     2761 cgtgtcgtac tggtcgcagc aggactcgta gtcgttcgcc gagacgacca tgtgcagcgg
     2821 gtcgttcggg tccacctcga tgtcctgctc gttcttcggg agccacgggt ggtcgcagtc
     2881 gaggttgtgg tcgctcggcc cgatcgtctc ggtcgcacac tcgatctccg agggcgccgg
     2941 attgtccacc cccgtcgagg ccccgttggc gttcgcgcga gcctgcgcca ggagcgccgc
     3001 gtccaccgag cgccgcccgg ccatcaggct ctgcggcttc gccgcctcgg cccccgggcc
     3061 gctcgctgcc aggatcgacg cgaacagcag cgcgcaggcc aggatcgtct ggagtcgtct
     3121 catcgcccct cctcggaaat ccagcagacg gcgccggtta tatcaggggg gcggtgccgg
     3181 gggaagggcc cgaatgggcc attccgccca tccgccgcgc ggagcgttcg gcccgccacg
     3241 ggtcgagcac ggggacgagc ggcgcgagcg ccggctccga cccgtagacg aggagtgcga
     3301 gcatcgcccc gtagaccggg aggtgcccga tcagctcgct cgccccgaag aagaagagcg
     3361 tggcgttgaa cgggattccg gtgaggacca cggcgatctg gggcgccgcc ccggagatca
     3421 ggagcagccc gaacaggacc tcgatgcccc cggcgatccg aacgaacatc aggtcggaga
     3481 ccggcagccc aagcgagcgg gccacgttca gcaccgggta ccggtccagg aactccaggg
     3541 ccaggtgcgg gtcgatcagc ttctcggtga acgccacgac gaggagcgag agcccgacca
     3601 gcatccgcag gtagaagacc gaccacagca ctcgcggccg ctcgggctcg gccgccccgt
     3661 accggtcacg cccgggcggg agcaccgcca ggaacagcgc gatgccgagc aggtcggccc
     3721 gctccaggat cgccaccggc ccgtacacca gcatccccag cggcccggcc aggaccagga
     3781 cgccggcggc gggccggatc cggaccccgg agatcagcca gacgccgagg actccctgga
     3841 ggatggcgag ggcgatcccg accgggttgt ccgccagccg catgctcggc gccaggtacg
     3901 tgccgcgcgc cgactgcgcc aggagcgata cgccggcgtg gatcgccaag aggcgcggga
     3961 cccacggcga caggcgcccg agagggcgca ggaaccccag ct
//
LOCUS       DASPWO010000496         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1001616, whole genome shotgun sequence.
ACCESSION   DASPWO010000496 DASPWO010000000
VERSION     DASPWO010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001616"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VEO00_11340"
     CDS             complement(<1..642)
                     /locus_tag="VEO00_11340"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYU58629.1"
                     /translation="MDRLAADYGPPLLLSEPVRGQYVPRELWRLPGYDQMRLWQERGD
                     EQPHIPLDHLLGIRMEDAGFDRASFVMPASPWLVSATGFILGGMLATVADAALGIAAY
                     VALPGMTAIATAELSMNFVRAATPESGVLRAHGGLIHAGGSLALSDTVVEDGAGKLLA
                     HGTSRVVVTRPRIPQEPVVEIPWEPAARYDTPDPFERRPVVGEVVPQETWDRLG"
     gene            complement(657..1748)
                     /locus_tag="VEO00_11345"
     CDS             complement(657..1748)
                     /locus_tag="VEO00_11345"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYU58630.1"
                     /translation="MIWEEPARGGYPDPRWFGLSGLEQMRAFLRNGGPRPPLSHLTGI
                     LPTEVGHGTSTFTMPVTGWLLPPPGLLGLGELAMLADAPLGTAIQTSLPPLTGYTTAE
                     LSMSHLRPVWPACGPLAARARLLQAGRSLGLSAVEILDGEGRLVAFGTSRCVIIPPMG
                     PPPAEPPPLEPVAVPDYASPDPFERPVVGAVVPQETWDRLSGLEVVQAYLKGELPPCP
                     LTELTGLRATEAEEGATTFVMPATEWLCSPLGTIQGGTVAMLADTAQVVAVQTTCPPR
                     TAYAPLDLKVNFLRPARPDGRDLTAHGRVVQRGRSIAVAHAEVTDVDGKRVALAVGTT
                     MILPGRAWSLERPFVPLDESGDPPAGEPG"
     gene            complement(1745..>2164)
                     /locus_tag="VEO00_11350"
     CDS             complement(1745..>2164)
                     /locus_tag="VEO00_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYU58631.1"
                     /translation="RIVVGSADAPAFRGEVPYPAGRGPLSLPYRPLAAFPRFEPDVTV
                     DGRTELDGGLVAIPTPGHTVGHISVWAPDLEALFVGDTIWNLARLRPSWKPFTQDLEA
                     NADSVAVLAGEKARRAYFGHGPAIGGDVPARFRRLAR"
BASE COUNT          297 a          792 c          822 g          253 t
ORIGIN      
        1 gccgagacgg tcccacgtct cctggggcac gacctccccg accaccggcc ggcgctcgaa
       61 cgggtccggg gtgtcgtagc gggcggccgg ctcccacggg atctcgacga ccggctcctg
      121 cgggatccgt ggacgggtga ccaccacccg ggacgtgccg tgggccagga gcttcccggc
      181 gccgtcctcc accacggtgt ccgagagcgc caggctcccg ccggcgtgga tcagcccgcc
      241 gtgggcgcgc aggacgccgc tctcgggcgt cgcggcgcgg acgaagttca tcgacagctc
      301 ggcggtcgcg atcgcggtca ttcccggcag cgcgacgtac gcggcgatgc cgagcgccgc
      361 gtcggccacg gtcgccagca tgccgccgag gatgaagccg gtggccgaga cgagccacgg
      421 cgaggcgggc atgacgaacg aggcccggtc gaacccggca tcctccatcc ggatcccgag
      481 caggtggtcg agggggatat gcggctgctc gtcaccccgc tcctgccaca gccgcatctg
      541 gtcgtagccc ggcagtcgcc acagctcgcg gggcacgtac tgcccgcgga ccggctcgga
      601 caggaggagg ggtggcccgt agtcggccgc gagccggtcc acctccacgg cgtgcttcag
      661 ccgggctcgc cggccggggg gtcgccggac tcgtcgagcg gcacgaacgg ccgctccagc
      721 gaccaggccc gccctgggag gatcatcgtc gtgccgaccg cgagggccac acgcttgccg
      781 tcgacatcgg tcacctcggc gtgcgccacc gcgatcgagc gcccgcgctg gaccacccgc
      841 ccgtgggcgg tgaggtcacg gccatccggg cgggcgggcc gcaggaagtt caccttcagg
      901 tcgagcgggg cgtaggccgt gcgcggcggg cacgtggtct ggacggccac gacctgggcc
      961 gtgtcggcga gcatcgccac ggtaccgccc tggatcgtcc cgagcgggct gcagagccac
     1021 tccgtggccg gcatgacgaa cgtggtcgca ccctcctcgg cctccgtcgc cctgagcccc
     1081 gtcagctccg tgagcggaca gggcggcagc tcgcccttca ggtacgcctg gacgacctcg
     1141 agccctgaca gccggtccca cgtctcctgc ggaacgacag ctcccaccac cggccgctcg
     1201 aacgggtcgg gcgaggcgta gtcggggacc gcgaccggct cgagcggggg cggctcggcc
     1261 ggcggcggcc ccatcggcgg gatgatcacg cagcgcgacg tcccgaaggc caccaggcgt
     1321 ccctcgccgt cgaggatctc caccgcggac agccccagcg agcggcccgc ctgcaggagg
     1381 cgggcccgcg cggcgagcgg cccgcacgcc ggccagaccg gccgcaggtg gctcatcgac
     1441 agttcggcgg tcgtgtagcc ggtgagcggc ggcagggacg tctggatggc ggtcccgagc
     1501 ggcgcgtcgg ccagcattgc cagctctccg aggccgagca ggcccggcgg cgggagcagc
     1561 cacccggtga cgggcatcgt gaacgtggag gtcccgtggc cgacctcggt cggcaggatc
     1621 ccggtcaagt ggctgagcgg gggacgcggg ccaccgtttc ggaggaacgc gcgcatctgc
     1681 tccaagccgg acaggccgaa ccaccggggg tcggggtatc ccccgcgggc gggctcctcc
     1741 cagatcaccg ggccagccgg cggaagcgcg ccgggacgtc gccgccgatg gccggcccgt
     1801 ggccgaagta ggcgcggcga gccttctcgc cggcgagcac ggccacggag tccgcgttgg
     1861 cctcgaggtc ctgcgtgaac ggcttccagg agggccgcaa ccgggcgagg ttccagatcg
     1921 tgtctccgac gaagagcgcc tcgagatcgg gcgcccacac cgagatgtgg ccgacggtgt
     1981 gccccggcgt cgggatggcg accagcccgc cgtccagctc ggtgcgtccg tccaccgtga
     2041 cgtcgggctc gaagcgcggg aaggccgcca ggggccggta gggcaggctg aggggaccgc
     2101 gtcctgcggg ataggggacc tcccctcgga acgcgggcgc gtcggccgac ccgaccacga
     2161 tccg
//
LOCUS       DASPWO010000497         3799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_411501, whole genome shotgun sequence.
ACCESSION   DASPWO010000497 DASPWO010000000
VERSION     DASPWO010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411501"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VEO00_11355"
     CDS             complement(<1..1112)
                     /locus_tag="VEO00_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYU58632.1"
                     /translation="MIDRNDIDRLAERVREVMAHWRKPGRTFGPEGMQEVGIALGRNV
                     EVRAPLGLAFDEDDRRILELTEGQQYALHFVMHRRRAAITGPAGSGKTLLAIALARCL
                     GEQGTRTLLTCFNKGLAGWLRRATAGTTNVDAEHFHALCVRLAHEAGLTVPDGGEQPD
                     GSPFFEDDLPDLLERAARALGPRYGAIIVDEAQDFRPHWWPALLALHREPDTGLLYLF
                     ADDNQNLYGGAVPEDLVEDRWHLPQNLRTTKAIHEFVTVFYRGDEPVTAGGPPGRDVE
                     VLGYRENEDLVRLLAIVLRNLVEERVPLGEIVVLTPSRRAKSPLRLRGSVDGFRLSES
                     EEPGAVLVSSIHGYKGLERQVVILAEVGDRHREDLD"
     gene            complement(1109..1561)
                     /locus_tag="VEO00_11360"
     CDS             complement(1109..1561)
                     /locus_tag="VEO00_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HYU58633.1"
                     /translation="MARMVPFPMLPTESSAERRLYEWFLHRLDDDYVVYHSVDWVLRG
                     PKGADEGEADFVVTHPRDGLLVVEAKGGGLAYDAGTRTWTQSGRGGTHRLDEDTRFTR
                     RETSCTTSRRSWRRARTGSGGSRRTATRWRSPTRCTTVRPIRAPRPRS"
     gene            complement(1576..2688)
                     /locus_tag="VEO00_11365"
     CDS             complement(1576..2688)
                     /locus_tag="VEO00_11365"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HYU58634.1"
                     /translation="MPQGRKRRVAVVFGGRSAEHEVSVVSARSVIDALDPERFEVVPI
                     GIDKGGRWHLLEGPPQLPSGTGGLPAVAEAGGKEVALSREPGERALVAEDGERAPFDV
                     VFPLLHGPFGEDGTIQGLLEMAGIPYVGSGVLASAVGMDKAVQKVLFRAAGLPVVEHV
                     AFTEADWEQDREEIEARAQALGLPLFVKPAALGSSVGVTKIRSRDRLVAAVEEAFAYG
                     RKALIERAMEGYREIECAVLGNDDPIASLPGEVVVRGAEFYDYRAKYLDDVARIDVPA
                     VLQPETVEAVQRLSVAAFRAVDAAGMARVDFFVREPDAVFVNEINTIPGFTSISMYPK
                     MWAASGVPYAELVERLVDLAVERHDREAKRGRTLPG"
     gene            complement(2688..>3799)
                     /locus_tag="VEO00_11370"
     CDS             complement(2688..>3799)
                     /locus_tag="VEO00_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYU58635.1"
                     /translation="ASVPIYQTSTYAFQRAEALADLIRAGKDAGFVYSRWHNPTRAAL
                     EEVVAELEGAERAVSFSSGMAAISTALGASAGAGDHVVSSPDLYGGTHSLMTKILPRW
                     GIETTLARSSTVEDLLFALRPNTRVCYVETIANPRLSVPDLEALGPECRARGVRLIVD
                     NTFASPYLCTPLDLGADVVVHSTTKYLGGHNDLIGGVAAGSAETMREVRELSVDLGGV
                     AGPVDAWLVLRGVQTLALRMERHCANAAALAAMLEGHPKVARVWYPGLASHPDHTVAR
                     RVLRGFSGMLSFELAGGLDAGVRFQDALEVALVGPSLGGVHTLVTHSASVTHTQLTAE
                     ERAAAGITEGFVRVSVGIEDAPDILDDFGRALEKA"
BASE COUNT          520 a         1451 c         1306 g          522 t
ORIGIN      
        1 tcgtcgaggt cctcgcggtg gcggtcgccg acctcggcca ggatcaccac ctgccgctcg
       61 aggcccttgt acccgtggat cgaggagacc agcaccgcgc cgggctcctc cgattccgaa
      121 agccggaacc cgtccaccga cccgcgcagc cggagcgggc tcttcgcccg acgggacggg
      181 gtcaggacca cgatctctcc gagcggcacg cgctcctcga ccaggttccg cagcacgatc
      241 gccagcagcc gcacgaggtc ctcgttctcg cggtagccga gcacctcgac gtcccggcca
      301 gggggcccgc ccgcggtgac gggctcgtcg ccccggtaga agaccgtgac gaactcgtgg
      361 atcgccttcg tggtgcgcag gttctgcggc aggtgccagc gatcctcgac caggtcctcg
      421 ggcaccgccc cgccgtacag gttctggttg tcgtcggcga acaggtagag cagcccggtg
      481 tcgggctcgc ggtgcagcgc caggagcgcc ggccaccagt gcggccggaa gtcctgcgcc
      541 tcgtcgacga tgatcgcccc gtaccggggc ccgagcgcgc gggccgcccg ttcgagcagg
      601 tcggggagat cgtcctcgaa gaacggcgac ccgtcgggct gctcgccgcc gtccggcacc
      661 gtgagcccgg cctcgtgggc caggcggacg cagagcgcgt ggaagtgctc ggcgtcgacg
      721 ttcgtggtgc ccgccgtggc ccggcgcagc cacccggcca gccccttgtt gaagcaggtc
      781 agcagcgtgc gcgttccctg ctccccgagg catctcgcca gcgcgatggc caggagcgtc
      841 ttcccgctcc cggccgggcc ggtgatggcg gcgcgccggc ggtgcatcac gaagtggagc
      901 gcgtactgct gcccctcggt cagctcgagg atccgccggt cgtcctcgtc gaaggccagc
      961 cccagcggcg cccggacctc cacgttgcgc cccagagcga tcccgacctc ctgcatcccc
     1021 tccggcccga acgtccgccc gggcttccgc cagtgcgcca tcacctcacg gacgcgctcg
     1081 gccagccgat cgatgtcgtt ccggtcgatc acgatctcgg ccggggcgcc cggatgggcc
     1141 ggacggtcgt acagcgcgtc ggggagcgcc agcgcgtagc cgtacgacgg cttccaccgc
     1201 tcccagtccg ggcgcgccgc caggatctcc tcgaggtcgt gcagctcgtc tctcgcctgg
     1261 tgaaacgggt gtcctcgtcc agccggtggg tgcccccccg ccccgactgc gtccatgtcc
     1321 gcgtccccgc gtcgtaggcc agcccgccac ccttcgcctc gaccaccagc aacccgtcgc
     1381 gcgggtgcgt gacgacgaag tcggcctcgc cctcgtccgc gcccttcggg ccgcgcagca
     1441 cccagtcgac gctgtggtac acgacgtagt cgtcgtccag gcggtgcagg aaccactcgt
     1501 agaggcgccg ttcggccgag ctctcggtcg gcagcatggg gaacgggacc atccgggcca
     1561 tcggcgctcc gtggttcagc cgggcagggt tcttccgcgc ttggcctcgc ggtcgtggcg
     1621 ctcgacggcg aggtcgacca gccgctccac cagctcggcg taggggacgc cggacgccgc
     1681 ccacatcttc gggtacatcg agatgctggt gaacccgggg atcgtgttga tctcgttcac
     1741 gaacaccgcg tccggctcgc ggacgaagaa gtccacccgc gccatcccgg cggcgtccac
     1801 cgctcggaag gcggccaccg acaggcgctg gacggcctcc accgtctcgg gctggaggac
     1861 cgccggcacg tcgatgcgcg cgacgtcgtc gaggtacttc gcgcggtagt cgtagaactc
     1921 ggcgccccgg acgacgacct cgccggggag gctggcgatc gggtcgtcgt tgccgagcac
     1981 cgcgcattcg atctcgcggt acccctccat cgcccgctcg atcagcgcct tgcgcccgta
     2041 ggcgaaggcc tcctcgaccg ccgccaccag ccggtcacgt gaccgtatct tcgtcacgcc
     2101 gaccgatgag ccgagggcgg ccggcttgac gaacaggggc agcccgaggg cctgcgcccg
     2161 cgcctcgatc tcctcgcggt cctgctccca gtcggcctcg gtgaacgcga cgtgctcgac
     2221 caccgggagc ccggccgcgc ggaacagcac cttctgcacg gccttgtcca tcccgaccgc
     2281 ggaggccagc acccccgacc cgacgtaggg gatgcccgcc atctccagga gcccctggat
     2341 cgtcccgtcc tccccgaacg gtccgtgcag caaagggaag accacgtcga acggggcgcg
     2401 ctcgccgtcc tccgccacga gcgcacgctc ccccggctcg cgcgacagcg cgacctcctt
     2461 gccgcccgcc tccgccaccg cgggcagccc tcccgtcccg gacgggagct gcggcggccc
     2521 ctccagcagg tgccaccggc cacccttgtc aatgccgatc gggacgacct cgaaccgctc
     2581 cgggtcgagc gcgtcgatca cgctccgggc ggagacgacc gacacctcgt gttcggccga
     2641 gcggccgccg aacacgaccg cgacgcgccg cttgcgcccc tggggcacta ggccttctcc
     2701 agggcgcggc cgaagtcgtc gaggatgtcc ggcgcgtcct cgatgcccac cgagacgcgg
     2761 acgaacccct cggtgatccc ggccgcggcg cgctcctccg ccgtgagctg ggtgtgcgtg
     2821 acgcttgccg agtgggtgac gagcgtgtgc acgccgccga ggctcggccc gaccagcgcg
     2881 acctccaggg cgtcctggaa ccgcaccccg gcgtcgaggc cgccggccag ctcgaacgag
     2941 agcatcccgg agaacccccg cagcaccctg cgcgccacgg tgtggtccgg atgtgaggcc
     3001 agtcccgggt accagacccg ggcgaccttc gggtgcccct cgagcatcgc cgccagcgcg
     3061 gcggcgttcg cgcagtgccg ctccatccgc agggcgaggg tctgcacgcc gcgcaggacg
     3121 agccacgcat cgaccggccc ggccacccct ccgaggtcga ccgagagctc gcgcacctcg
     3181 cgcatcgtct ccgccgaccc ggccgccacg ccgccgatca ggtcgttgtg gccgcccagg
     3241 tacttcgtgg tcgagtgcac gaccacgtcg gcgccgagat cgagcggcgt gcagaggtag
     3301 ggggaggcga aggtgttgtc gacgatcagc cgcacgccgc gggcgcggca ctcggggccg
     3361 agcgcctcga ggtcggggac gctcagcctg gggttggcga tcgtctccac gtagcagacc
     3421 cgcgtgttcg gccgcagggc gaacagcagg tcctcgaccg tcgacgatcg cgccagcgtg
     3481 gtctcgatcc cccaccgcgg aaggatcttc gtcatcagcg agtgggtgcc gccgtacagg
     3541 tccgggctgg agaccacgtg atcgccggcg ccggcggacg cgccgagcgc ggtcgagatg
     3601 gcggccatcc cggacgagaa ggagacggcg cgctcggctc cctccagctc ggcgacgacc
     3661 tcctccaggg ccgcccgcgt cgggttgtgc cagcgcgagt agacgaaccc ggcgtccttc
     3721 ccggcccgga tcagatcggc gagggcttcg gcccgttgga acgcgtacgt cgaggtctgg
     3781 tagatgggta ccgacgcgg
//
LOCUS       DASPWO010000498         8944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_750396, whole genome shotgun sequence.
ACCESSION   DASPWO010000498 DASPWO010000000
VERSION     DASPWO010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750396"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VEO00_11375"
     CDS             <1..740
                     /locus_tag="VEO00_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HYU58636.1"
                     /translation="YIIDTGIRITHSLFGGRASYGYDAVDGSLPADDCNGHGTHVSGT
                     VGSSTYGVAKGVSLVAVRVLNCQGSGTYAQVIAGVNWVTGDHLTGQPAVANMSLGGGP
                     SASLDTAVKNSITDGVTYAIAAGNSNRKACNYSPARVAAALTVGATTSTDARASYSNW
                     GTCLDLFAPGSSITSTWNTSDTATNTISGTSMATPHVAGVAALYLQGNPTASPATVGN
                     TVISSATLGKVTHRRTGSPNRLLYSLL"
     gene            795..2402
                     /locus_tag="VEO00_11380"
     CDS             795..2402
                     /locus_tag="VEO00_11380"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HYU58637.1"
                     /translation="MAKTEPREQELPPEPPAAPHTIEERLADLDRKRAEALEAGGPGS
                     IEKQHARGKLTARERIDLLLDPGSFVETDQLARHRATSFGIDTKRPYGDGVVTGWGTI
                     DGRKVFIFSQDFTVFGGSLGEVFAEKVCKVMDLAMAVGAPVIGINDSGGARIQEGASS
                     LAGYGYIFDRNVRASGVIPQISLVMGPCAGGAVYSPAMTDFVYMVKETSHMFITGPEV
                     IKTVTGEDVTFEELGGAMTHAQRSGVANFVAENEEDCLAQVRYLMSFLPSNNMEDPPA
                     YATTDDPERRDESLRHLIPESPREPYDMRDVIERVFDDGGFYEVSALWAKSILTGFAR
                     LDGRSVGIVANQPEVLAGTLDMESSEKAARFVRFCDAFNVPLVTFVDVPGFLPGVTQE
                     YGGIIRRGAKLLYAFTEATVPRVTVITRKAYGGAYVVMNSKHIRADVSYAWPTAEIAV
                     MGSEGAVNIVHRRDIAGSEDPDARREQLIAEYEERFSNPYIAAERGYVDAVIDPADTR
                     PLLIRAFRMLHTKREAVPPRKHGNIPL"
     gene            2489..2734
                     /locus_tag="VEO00_11385"
     CDS             2489..2734
                     /locus_tag="VEO00_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58638.1"
                     /translation="MELAEGLPPEEAAAVRDALRRYLEGLRPRLSPWVVAGRAENTRQ
                     GALQFRYQDDRAWQRVGHDFIFARQVSEARMGRGDSK"
     gene            2746..3951
                     /locus_tag="VEO00_11390"
     CDS             2746..3951
                     /locus_tag="VEO00_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYU58639.1"
                     /translation="MDEGDRPLPLEGVRVLDLATIIAGPGVSARLGDFGADVVKVEHP
                     RGGDTLRGLGWKVAGVALWWKLIGRNKRPITLDLSHPKGQDLLLRLVDGADVLIESFR
                     PGTLERWNLAPERLAERNPRLVVLRVSGFGQTGPYAPRPGFGTLAEALSGYVHLTGEP
                     GEAPVLPPVALADEVSALLGAYAVMIALYHRDVRGGGGQMIDLSLIESLFGVTGPVAT
                     VYDKVGLVEGRHGNRMPYAAPRGAFRTEDGRWVALSGTSQSVAERVFAAIGRSDLVAD
                     PRFATNEARVEHVEELDDILWDWMEAHTLAEVMAAFEAHEAAVAPIYDVAQIAADVHY
                     RARGTLVRVPDEELGDLVLPDPQPRLSATPGRIRHAGLPLGAANADVYGELGLGPDDL
                     DALRLDGAI"
     gene            4091..4411
                     /locus_tag="VEO00_11395"
     CDS             4091..4411
                     /locus_tag="VEO00_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYU58640.1"
                     /translation="MTAIRTANEGPAPLSLGEIQDIFRAEGLSAPRFWSNGPGDTYGR
                     HEHSYHKVLYCLEGSIVFHTDDGDLALGPGDRLDIAPGTAHAATVGTDGVSCVEASRE
                     AGPG"
     gene            4408..5520
                     /locus_tag="VEO00_11400"
     CDS             4408..5520
                     /locus_tag="VEO00_11400"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYU58641.1"
                     /translation="MTSVAWGVDQVFAAVPGGIGTYVRELVPALAAQDPSLRITLFHA
                     RVDDARPEPWMADFRTETLSGTIQRLYPSWNLAGRPALPPSLASHDVVHVPSCVAVPP
                     TGPGRRLVVTVHDLAFRLYPDLFPPAWRAMFRLGLRRAVRTADAIVTVSRNTAEDLVR
                     LTKVDPAKLHVVPLAASLPVSPLAVEPLLERLKVRRPYLLSVGTLEPRKNLVRLVRAY
                     RRAAAEGVPHVLVLAGPMGWGPQPLLRELRVPGPGEVVLTGAMAPDELDALYRGAAAF
                     VYPSLYEGFGLPVVEAMSRGLPTVVSDVSSLPEVAGEAAIYVHPGSVRDLARAMQHAV
                     EPEEAERLGVAARARADRFSWAETARLTLEVYQKAG"
     gene            5517..6443
                     /locus_tag="VEO00_11405"
     CDS             5517..6443
                     /locus_tag="VEO00_11405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU58642.1"
                     /translation="MKVSLITTVKDAELAVEPFLASVRAQSRAPDEVVVVDGGSADGT
                     VAALRRHPDLVLVEEHRANIAMGRNVAIKTAVHDVIAATDGDCILHPEWLARVVAPVE
                     DGAAVGMGFTRPAPRSFLEACVAAAHLPDAGEVDPAHFMPSARSVAFRRSAIEAVGGY
                     PEWLDIGEDMWVDHRWRELGLDMRFVPDAVVYWHPRPDLAGLWRQFAGYAEGDARAGM
                     YPGRHLIRAGAYAAGALAWASGRPGARAVVAAAGLWYARRPLRRAWRRLAGRPRERAA
                     ALAAVPAVMAVSDAAKLWGYVRGLTAPIRPRR"
     gene            complement(6424..7137)
                     /locus_tag="VEO00_11410"
     CDS             complement(6424..7137)
                     /locus_tag="VEO00_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58643.1"
                     /translation="MPKKKPTQGDAKDTPKGAEPAPRNVPAGGSGGGRVAAIAGIAGA
                     GAAAIGAAADSVTLTEAFAGGDEESSPRPRAARGRDERERPARQRDFDRDAAADLDEP
                     DDPGAFVGTPAVLGNIPLITEGLPAEEDLGLDDDLGLGMDAAPAGGSEPEPPALDDPM
                     PDGEDGMEASTEPPPEAPSEPEPEPFQGTVPEAPPALEEAPLQDAPVLEEDAAPPPAE
                     DVEQPEPVEPVDDLSGEAG"
     gene            complement(7197..8045)
                     /locus_tag="VEO00_11415"
     CDS             complement(7197..8045)
                     /locus_tag="VEO00_11415"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYU58644.1"
                     /translation="MRAIDLHVHLPTEEWLAGAIGPNLAATEAYFRTAPERKTIEQVA
                     EEYAERDILGVVLDWDDETVTGRPWLGNAFLAGLAERFPGVLMGFGSVDPHRADAADR
                     VVEAADLGLAGLKFHPTMQAFDPADERFYPIWGKAEELGLVALFHTGTCGIGAGTPGA
                     GGTKIRYSHPGFLDAVGADFPGLTLLAAHFGWPWFLECLAIALHKANVWIELSGWAPK
                     YLPPEVVREAGRRLNGRTVFGSDYPFISLDRWFAEAEALPWTEEARRAILVENAARLL
                     GVGAGA"
     gene            complement(8045..8308)
                     /locus_tag="VEO00_11420"
     CDS             complement(8045..8308)
                     /locus_tag="VEO00_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58645.1"
                     /translation="MLLLAPLPGVQEPAGEAGTIPSGSAAVRVLMGYGDEMTSAGERD
                     VMLDGTLRGASVSGTFAAVACGPPDGPPDSDLVVRGTWEAHKT"
     gene            complement(8362..>8944)
                     /locus_tag="VEO00_11425"
     CDS             complement(8362..>8944)
                     /locus_tag="VEO00_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HYU58646.1"
                     /translation="ENLDTKLSIFRRLDEITGPEVVLASNTSSLPLVQMATATKRPDR
                     VVGMHFFNPPPVMKLLELVRAITTSDETVEFARAYGTDRLGKTTVLAKDRAGFIVNYL
                     LTTYLNAAVRMYEEGFATREDIDTAIKLGLGHPMGPLELLDLIGLDTMLSVGDVLYNE
                     FKAPEFNAPPLARRMVQAAHLGRKTGRGFYDYT"
BASE COUNT         1195 a         3208 c         3283 g         1258 t
ORIGIN      
        1 cctacatcat cgacaccggc atccggatca cgcactcgct gttcggggga cgggcgtcgt
       61 acgggtacga cgccgtggac ggctccctgc cggccgacga ctgcaacggc cacggcaccc
      121 acgtctccgg aaccgtcggc agcagcacgt acggcgtggc gaagggcgtc tcgctcgtgg
      181 cggtgcgcgt gttgaactgc cagggcagcg gcacgtacgc gcaggtcatc gccggggtca
      241 actgggtcac cggagaccac ctgaccggcc agccggcggt ggccaacatg agcctcggcg
      301 gcggcccctc ggcgtcgctc gacaccgccg tgaagaactc gatcacggac ggcgtgacgt
      361 acgcgatcgc ggccggcaac agcaatcgca aggcctgcaa ctactcgccc gcgcgggtgg
      421 ccgcagccct aacggttggg gccaccacct ccaccgacgc ccgggcgtcg tactcgaact
      481 ggggcacctg tctcgacctg ttcgcgccgg gatcgagcat cacctccacg tggaacacga
      541 gcgatacggc gacgaacacg atcagcggga cctcgatggc aactccccac gtcgccggag
      601 tggccgccct gtacctgcag gggaacccga cggcctcacc ggccacggtg gggaacacgg
      661 tcatcagcag cgccacgctg ggcaaggtga cccaccgccg gacaggttcg ccgaatcgcc
      721 tgctgtattc gttgttgtga ggaccccgcc acatcgccgc cctccggggc ccggcgggta
      781 gactcggctg gcgtatggcg aagaccgaac cgcgcgaaca ggagcttccg cccgagcctc
      841 ccgccgcgcc gcacaccatc gaggaacgcc tggccgacct cgaccgcaag cgggccgagg
      901 cgctcgaggc cggcgggccg ggctcgatcg agaagcaaca cgcccgaggc aagctgaccg
      961 cgcgcgagcg catcgacctg cttctcgacc cgggctcgtt cgtcgagacc gaccagctgg
     1021 cgcggcaccg ggccacgagc ttcgggatcg acacgaagcg cccctacggc gacggcgtgg
     1081 tcaccggctg gggaacgatc gacgggcgga aggtcttcat cttctcgcag gacttcacgg
     1141 tgttcggcgg gtccctcggc gaggtgttcg ccgagaaggt ctgcaaggtg atggaccttg
     1201 ccatggcggt cggcgccccg gtgatcggca tcaacgactc gggcggtgcg cggatccagg
     1261 aaggcgcgtc gtcgctggcc ggctacggct acatcttcga ccgcaacgtt cgagccagcg
     1321 gggtgatccc gcagatctcg ctggtcatgg gcccgtgcgc cgggggcgcc gtgtactcgc
     1381 cggcgatgac ggacttcgtg tacatggtca aggagacctc ccacatgttc atcaccgggc
     1441 ctgaagtgat caagacggtg accggtgagg acgtcacctt cgaggagctc ggcggcgcca
     1501 tgacccacgc gcagcgctcg ggcgtcgcca acttcgtggc ggagaacgag gaggactgct
     1561 tggcgcaggt tcgctacctg atgtcgttcc tgccctcgaa caacatggag gacccaccgg
     1621 cgtacgccac caccgacgac ccggagcgcc gcgacgagtc cctgcgccac ctgatccccg
     1681 agtcaccgcg cgagccgtac gacatgcgcg acgtcatcga gcgcgtcttc gacgacggtg
     1741 ggttctacga ggtctcggcc ctgtgggcga agagcatcct gaccgggttc gcgcgcctcg
     1801 acgggcggag cgtcggcatc gtggcgaacc agccggaggt actggccggg acgctcgaca
     1861 tggaatcctc ggagaaggcg gcgcggttcg tgcgcttctg cgacgcgttc aacgtcccgc
     1921 tggtgacgtt cgtcgacgtg ccggggttcc tgccgggcgt cacccaggag tacggcggga
     1981 tcatccgccg cggggcgaag ctgctctacg cgttcaccga ggccacggtg ccccgcgtga
     2041 cggtgatcac tcgcaaggcc tacggcggcg cgtacgtcgt catgaactcg aagcacatcc
     2101 gggccgacgt ctcctacgcc tggccgacgg ccgagatcgc ggtgatgggg tccgagggcg
     2161 cggtgaacat cgtccaccgg cgcgacatcg ccggctcgga ggatcccgac gcgcggcggg
     2221 agcagctgat cgccgagtac gaggagcgct tctcgaaccc ctacatcgcc gccgagcggg
     2281 ggtacgtcga cgccgtgatc gacccggccg acacccggcc gctgctgatc agggcgttcc
     2341 gcatgctgca cacgaagcgc gaggcggtac cgccccgcaa gcacgggaac atcccgctgt
     2401 gagccccgca gggcttgggg gggtggctcg ccccgggtgg gatgaacgcc ggtcaccggc
     2461 cgcgagcgga ccccccaagg aaggacgggt ggagcttgcg gagggactgc cgcccgagga
     2521 ggccgcggcg gtccgcgacg cgctgcgccg gtacctggag ggattgcgcc cgcggctgtc
     2581 gccgtgggtc gtcgccggcc gggcggagaa cacgcgccag ggcgcgctgc agttccgcta
     2641 ccaggacgac cgcgcctggc agcgcgtcgg ccacgacttc atcttcgccc gccaggtgtc
     2701 cgaagcgcgc atgggccgcg gcgactcgaa gtagcccgaa acggcatgga cgagggcgac
     2761 cggccgctgc cgctcgaggg ggttcgcgtg ctcgacctgg cgacgatcat cgccgggccg
     2821 ggggtgtcgg cgcggctcgg ggacttcggg gcggacgtgg tcaaggtgga acatccgcgc
     2881 ggcggggaca ccttgcgcgg gctcgggtgg aaggtcgccg gcgtggcgct gtggtggaag
     2941 ctgatcgggc gcaacaagcg gccgatcacg ctggacctgt cgcatccgaa gggacaggac
     3001 ctgctgctgc gcctggtgga cggcgcggac gtcctgatcg agtcgttccg cccggggacg
     3061 ctggagcgct ggaacctcgc gccggagcgg ctggcggagc gcaacccgcg cctggtggtc
     3121 ctgcgtgtct cggggttcgg gcagacaggg ccgtacgcgc cgcgcccggg gttcgggacg
     3181 ctcgccgagg cgctctcggg gtacgtccac ctgaccgggg agccgggcga ggcaccggtc
     3241 cttccgccgg tggcgttggc cgacgaggtc tcggcgctgc tcggcgcgta cgcggtgatg
     3301 atcgcgctgt accaccgcga cgttcgcggg ggtggcggcc agatgatcga cctgtcgctc
     3361 atcgagtcgc tgtttggcgt gacgggtccg gtcgccaccg tgtacgacaa ggtgggcctg
     3421 gtcgaggggc ggcacgggaa ccgcatgccg tacgcggcgc cgcgcggcgc cttccggacg
     3481 gaggacggtc ggtgggtggc gctgtcggga acgtcgcagt cggtcgccga gcgcgtcttc
     3541 gccgcgatcg gccgttccga cctggtcgcc gacccgcggt tcgccacgaa cgaggcccgc
     3601 gtcgagcacg tcgaggagct cgacgacatc ctgtgggact ggatggaggc gcacaccctg
     3661 gccgaggtga tggccgcgtt cgaggcgcac gaggcggcgg tcgccccgat ctacgacgtc
     3721 gcgcagatcg cggccgacgt ccactaccgg gcgcgcggga cgctggtccg agtgcccgac
     3781 gaggaactcg gtgacctggt gctccccgac ccccagccgc gcctgtcggc gacgcccggc
     3841 cggatccgcc acgccgggct cccgctcggc gcggcgaacg cggacgtcta cggcgagctg
     3901 ggcctcggcc ccgacgacct ggacgcgctg cgcctggatg gggcgatcta gcttctcagg
     3961 ttgcgggtcc acgaccccac ggccggcgat cgaacggccg cggtcgagcg accggcgaac
     4021 cggaataccc accggcgcct gggcgcgtcc gtccgaacct tcggcatccg gcctgactaa
     4081 gctctcccgc gtgacggcca tccggacggc gaacgagggg ccggccccgc tctcactggg
     4141 ggagatccag gacatatttc gggccgaggg actgtcggcg ccgcggttct ggtcgaacgg
     4201 acccggggat acgtacggca ggcacgagca ctcttaccac aaggtcctgt actgcctgga
     4261 gggctcgatc gtgttccaca ccgacgacgg ggatctggcg ctcgggcccg gcgaccgcct
     4321 ggacatcgcg cccggaacgg cacacgcggc gacggtcggc accgacggcg tgtcgtgcgt
     4381 cgaggcatcg cgggaagccg gccccggatg acgagcgtcg cgtggggcgt cgaccaggtc
     4441 ttcgccgccg tgcccggcgg gatcggaacc tacgtccgcg agctggtgcc ggcgctcgcc
     4501 gcgcaggacc cctcgctgcg gatcacgctg ttccacgcgc gtgtcgacga cgcccgcccc
     4561 gagccgtgga tggccgactt ccggacggag acgctcagcg gaacgatcca gcggctctac
     4621 ccgtcgtgga acctggccgg ccgtccggcg ctccccccct cgctcgcgtc gcacgacgtc
     4681 gtccacgtcc cgtcctgcgt ggcggtccca ccgaccggtc ccggccggcg attggtggtc
     4741 acggtgcacg acctggcctt ccgcctctac cccgacctct tccctccggc gtggcgggcg
     4801 atgttccgcc tcggcctgcg ccgcgcggtg cgcaccgccg acgccatcgt cacggtctcg
     4861 cggaacacgg ccgaggacct ggtccggctg acgaaggtcg acccggcgaa actccacgtg
     4921 gtgccgctgg ccgcgtcgct gccggtctcc ccgctcgcgg tcgagccgct cctcgagcgg
     4981 ctgaaggtcc gccgccccta cctgctctcg gtcgggacgc tcgagccgcg caagaacctg
     5041 gtccggctgg tgcgggcgta ccggcgggcc gccgccgagg gcgtgccaca cgtgttggtg
     5101 ctggccgggc cgatggggtg gggcccccag ccgttgctcc gcgagctgcg cgtgccgggc
     5161 cccggcgaag tggtcctgac cggggcgatg gcgccggacg agctcgatgc cctgtaccgc
     5221 ggcgccgccg cgttcgtcta cccctcgctc tacgaggggt tcgggcttcc cgtcgtcgag
     5281 gcgatgagcc gcggcctccc gaccgtggtg tcggacgtct cctcgctgcc cgaggtggcc
     5341 ggtgaggccg cgatctacgt ccaccccggc tccgtgcgcg acctggcccg ggcgatgcag
     5401 cacgcggtcg agccggagga ggccgagcgg ctcggcgtcg ccgcccgggc gcgcgccgac
     5461 cggttctcgt gggccgagac ggcgcggctg accctggagg tgtaccagaa ggccggatga
     5521 aggtctcgct gatcaccacc gtgaaggacg ccgagttggc cgtcgagccg ttcctggcct
     5581 cggtccgggc gcagtcgcgg gcgccggacg aggtcgtggt cgtcgacggc gggtccgccg
     5641 atgggacggt ggccgcgttg cgccgccatc cagacctcgt gctggtcgag gagcaccggg
     5701 cgaacatcgc catgggccgc aacgtggcga tcaagacggc cgtgcacgac gtgatcgcgg
     5761 cgaccgacgg cgattgcatc ctgcacccgg agtggctggc acgggtcgtc gccccggtcg
     5821 aggacggcgc cgccgtgggg atggggttca cgcgcccggc gccgcgctcg ttcctggagg
     5881 cgtgcgtcgc cgccgcgcac ctgcccgacg ccggcgaagt ggacccggcg cacttcatgc
     5941 cgtcggcgcg ctcggtggcc ttccggcgca gcgcgatcga ggccgtcggc ggctaccccg
     6001 agtggctcga catcggcgag gacatgtggg tggaccaccg ctggcgcgag ctgggcctgg
     6061 acatgcgctt cgtcccggac gcggtcgtct actggcaccc gcggcccgac ctggccggcc
     6121 tctggcgaca gttcgccggc tacgcggagg gcgacgcgcg ggccgggatg taccccgggc
     6181 gacacctgat ccgcgccggc gcgtacgcgg cgggcgcgct ggcgtgggcg tcgggccgcc
     6241 ccggggcgcg cgccgtcgtg gcggcggccg gcctctggta cgcacgccgt cccctgcgcc
     6301 gcgcctggcg ccggctcgcc ggccgtccgc gcgagcgcgc cgccgcgctg gccgcggtcc
     6361 cggccgtgat ggcggtgagc gacgccgcga agctgtgggg atacgtgcgg ggcctgacgg
     6421 ctcctatccg gcctcgccgc tgaggtcgtc aaccggttcg accggctccg gctgctccac
     6481 gtcctccgcc ggcggcggtg cggcgtcctc ctcgaggacg ggggcatcct ggaggggagc
     6541 ctcctcgagc gctggcggcg cctcgggcac ggtcccctgg aacggctccg gctccggctc
     6601 gctgggggcc tccggcggtg gctcggtcga ggcctccatg ccgtcctcgc cgtccggcat
     6661 cgggtcgtca agggccggcg gctccggctc ggaacctccg gcgggcgcgg cgtccatgcc
     6721 gagcccgagg tcgtcatcga gtccgaggtc ctcctccgcc ggcagaccct cggtgatcag
     6781 cgggatgttg ccgagcaccg cgggggtgcc gacgaacgct ccgggatcgt cgggttcgtc
     6841 gaggtcggcg gccgcgtccc ggtcgaagtc gcgctggcgg gcggggcgct cgcgctcgtc
     6901 ccgtccccgt gccgcccgcg gccggggtga tgactcctcg tcgcccccgg cgaacgcctc
     6961 ggtcagcgtc acgctgtcgg cggccgcccc gatggctgcg gccccggcgc cggcgatccc
     7021 ggcgatcgcg gcgacgcgcc cgccaccgct gccgcccgcg gggacgttcc ggggcgcggg
     7081 ttccgcgccc ttcggcgtgt ccttcgcgtc gccctgggtg ggcttcttct tcggcacggc
     7141 gaggtcctgt ctgttggggg gatcctggcc agactaggaa ccggaccggt ccgcggtcaa
     7201 gcgccggcgc cgaccccgag caggcgggcg gcgttctcca ccaggatcgc gcggcgggcc
     7261 tcctcggtcc acggcagcgc ctccgcctcg gcgaaccagc ggtccaggga gatgaacggg
     7321 tagtcgctgc cgaacaccgt gcgcccgttc aggcgcctgc cggcctcgcg caccacctcc
     7381 ggcggcaggt acttcggcgc ccagcccgac agctcgatcc agacgttcgc cttgtgcagc
     7441 gcgatggcca ggcactccag gaaccagggc cagccgaagt gcgcagcgag cagcgtcagc
     7501 ccggggaagt ccgccccgac cgcgtcgagg aaccccggat gggagtagcg gatcttcgtc
     7561 ccgccggcgc cgggcgtgcc ggcgccgatg ccgcaggtcc cggtgtggaa gagcgccacc
     7621 aggcccagct cctccgcctt gccccagatc gggtagaagc gctcgtcggc ggggtcgaac
     7681 gcctgcatgg tcgggtggaa cttcagcccc gcgagaccga ggtccgcggc ctccacgacg
     7741 cggtcggcgg cgtcggcccg atgtgggtcc accgacccga accccatcag caccccgggg
     7801 aaccgctcgg ccagcccggc gaggaaggcg ttcccgagcc agggccggcc ggtgacggtc
     7861 tcgtcgtccc agtcgaggac caccccgagg atgtcgcgct cggcgtactc ctccgccacc
     7921 tgctcgatcg tcttgcgttc cggcgccgtg cggaagtacg cctcggtcgc cgcgaggttc
     7981 ggcccgatgg cgcccgccag ccactcctcg gtcggcagat gaacgtgcag gtcgatggcc
     8041 ctcatcacgt cttgtgggct tcccaggtgc cccgaacgac gaggtccgaa tccggcggcc
     8101 cgtcgggggg tccgcaggcc accgccgcga aggtgcccga caccgacgct ccccggagcg
     8161 tgccatcgag catgacgtcc cgctcccccg cggaggtcat ctcgtccccg taccccatca
     8221 gcacccgcac cgccgccgag cccgatggga tcgtcccggc ttcgccggcg ggctcctgga
     8281 cgccgggcag cggcgcgagg aggagcacgc gccgatggtc accgccgcga tgacggcggc
     8341 gagccgcagc cgccgcatcg cctacgtgta gtcgtagaag ccccggccgg tcttgcggcc
     8401 gaggtgggcg gcctggacca tccgccgggc gagcggcggc gcgttgaact ccggcgcctt
     8461 gaactcgttg tacagcacgt cgccgacgga gagcatcgtg tccagcccga tcagatcgag
     8521 cagctcgagc ggccccatcg gatggccgag gccgagcttg atcgcggtgt cgatgtcctc
     8581 gcgcgtggcg aagccctcct cgtacatccg cacggccgcg ttgaggtagg tcgtcagcag
     8641 gtagttgacg atgaagccgg cgcggtcctt cgccagcacg gtggtcttcc ccagccggtc
     8701 ggtcccgtag gcgcgcgcga actcgacggt ctcgtccgac gtcgtgatcg cccgcaccag
     8761 ctcgagcagc ttcatcaccg gcggggggtt gaagaagtgc atccccacca cccggtcggg
     8821 tcgcttcgtc gcggtggcca tctgcaccag cggcagcgac gacgtgttcg aggcgagcac
     8881 gacctcgggc ccggtgatct cgtcgagccg gcggaagatc gagagcttgg tgtcgaggtt
     8941 ctcg
//
LOCUS       DASPWO010000499         6670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1002008, whole genome shotgun sequence.
ACCESSION   DASPWO010000499 DASPWO010000000
VERSION     DASPWO010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002008"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VEO00_11430"
     CDS             complement(<1..567)
                     /locus_tag="VEO00_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HYU58647.1"
                     /translation="MTREWAADFEALRATDPEVFEAIANELDRERRTLRLIASENYAS
                     PAVLAALSSTMNNKYAEGYPGRRYYGGCEFVDVSETLAIERARQLFGAEHANVQPHAG
                     AQANLAVYGAFVQPHSEDTVLGLVLAHGGHLTHGSPVNVSGKWFRFVGYEVDPQTELI
                     DMDRVRDLAREHRPKIILAGFTAYPRTID"
     gene            complement(564..1022)
                     /gene="rpiB"
                     /locus_tag="VEO00_11435"
     CDS             complement(564..1022)
                     /gene="rpiB"
                     /locus_tag="VEO00_11435"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HYU58648.1"
                     /translation="MTGFRVAIGSDHAGYRLKEELKAFLKEEGYEVADLGTDSEEPVD
                     YPPICAAVGREVAAGRAERGIMLGGSGQGEQIAANKVRGVRAALCNDLYTARMSRRHN
                     DANVLAIGARIVAPELAREIVTTWFRSDFEEGRHVRRLEQIAAIEEEECR"
     gene            complement(1024..1623)
                     /locus_tag="VEO00_11440"
     CDS             complement(1024..1623)
                     /locus_tag="VEO00_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HYU58649.1"
                     /translation="MPSVLFVCTGNICRSPMAEGLLREHLRHRFPEKPFEVSSAGLIA
                     VDGHRAMLEAVRAAEEREADISEHRARRLRPEHVEAADLVIGMAVEHVEAVYEMVPDA
                     AGKLFTLRELVEILKALPPLDDPRALDDGRFRDRVAEAAARRRVAPPPPYDLDIADPL
                     GMPFETYRATAWQLDNLLGQLIEGIAGRQPARTSMWEDE"
     gene            complement(1634..2287)
                     /locus_tag="VEO00_11445"
     CDS             complement(1634..2287)
                     /locus_tag="VEO00_11445"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009400717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HYU58650.1"
                     /translation="MTVLVVDPADPDPDDIARAAAALAAGQLVVMPTDSVYGLAADPA
                     LPAAVSRLFEAKGRPRSLSIPVLVASMSHVDRLVEVDDRARRLIERYWPGPLTIVLPR
                     RPGVDWELGEARATLAVRMPDHPVALALLTRTGPLAVTSANRSGESTPATVPEIEAVL
                     GDRAGVYLDAGPAPLGTPSALVSLVGPALVLREGPIDLAELMVTALQGTPEGGRATR"
     gene            complement(2284..3123)
                     /gene="prmC"
                     /locus_tag="VEO00_11450"
     CDS             complement(2284..3123)
                     /gene="prmC"
                     /locus_tag="VEO00_11450"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HYU58651.1"
                     /translation="MRARPVEVLNRAADYLERHGVEAPRETAEALLMRVLGTDRAEIY
                     TRTAMDMASAKAFGRALCQRCSGTPLQHLTGAQPFRTLRLAVEPGVFVPRPETEVVVE
                     AALDAIAGIDAPLVVDAGTGTGAIALSVAAEHPGAIVHATDLNPAAVALARRNAESHG
                     LAVVVREGDLLEPLPAALRGRVELVVSNPPYVSAAEYADLPPEVRADPREALVGGTEV
                     HRRLAAAARDWLLPGGALVMEIGASQAEEVRSILADTGYAQIEILPDLALRDRVAGAR
                     TGS"
     gene            complement(3129..4217)
                     /gene="prfA"
                     /locus_tag="VEO00_11455"
     CDS             complement(3129..4217)
                     /gene="prfA"
                     /locus_tag="VEO00_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HYU58652.1"
                     /translation="MNAQVRERLEGIERRFEELGAELSLPETAADPAKLRGLGKQHAE
                     LEPVVRAFRAYRGAAAQAEEGLALAKGEADPDMAEYFRTEADQAAARAAELERELEIL
                     LLPKDPNDDRNVIIEIRAGTGGDEAALFAGELLEMYRRFAERHRWKTEVLSSNPSDLG
                     GVKEVVLAARGDRAFSRMKHEAGVHRVQRVPVTESGGRIHTSTATVAVLPEAEETEVD
                     IKRDELQIDVFRSSGPGGQSVNTTDSAVRVTHRPTGIVVSVQEERSQMQNRDKAMRYL
                     RARLLQLAQREAAAKEAAARSAQVGTGERSEKIRTYNFPQNRVTDHRIGLTVHNLPDV
                     LAGNLDPFVDALMANERAEQLGRQNGEG"
     gene            complement(4528..5502)
                     /locus_tag="VEO00_11460"
     CDS             complement(4528..5502)
                     /locus_tag="VEO00_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HYU58653.1"
                     /translation="MAAETGPALDPVGPEPQPPHFYGGQAVLEGVMMRGKDHWALAVR
                     RPDTSIHLEAHHIDSVANRYRVLRWPGFRGVIGLGQALAIGVKALTASANQSAEEEAK
                     LTRGQMAFSMTLALVLFIGLFVVGPAVLFSFFRRTVDSSILRNVLEGLFRVALFLGYL
                     ALIGRMKDIRRVFQYHGAEHKTIAAFEHGEPLVPERVDRFSTLHVRCGTNFLLIVMIL
                     TILVFALFGNPPILWAILSRIVAIPVIAGIAYELLRLGAKYTHSPVMRFLMAPGLWLQ
                     KITTRPPSDDQIEVAIASFQEVLRAEGKLPPRTLDETGAAQEPATSDS"
     gene            complement(5510..5713)
                     /gene="rpmE"
                     /locus_tag="VEO00_11465"
     CDS             complement(5510..5713)
                     /gene="rpmE"
                     /locus_tag="VEO00_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HYU58654.1"
                     /translation="MKQGIHPEYVVATVHCSCGNTFVTRSTKPEIRVEICSNCHPFYT
                     GKQKLVDTGGRVERFQRRYAKRG"
     gene            5819..>6670
                     /locus_tag="VEO00_11470"
     CDS             5819..>6670
                     /locus_tag="VEO00_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58655.1"
                     /translation="MSSDESLPHPSLGVSEEEAQARFEALQAKLVPLWRSIAALNQDP
                     QTIVVVPSLTLDQLEEHGVEMQAYEERYLFLLFLLRQPRARMVYVTSQKIAPNILDYY
                     FHLLPGVISSHARKRFFNPAPQDGAARALSQKLLERPRLLEEIRGLILDPDRAHMVPY
                     NTTTLERDVALRLGIPMYAADPKFFPLGTKSGCRKLFAEEGVPHPLGHEDLRGEDDLV
                     DALVKMRAERPAMQRAIVKTNEGVSGGGNAHVDLAGTPAPGDPAERDAVVDRVRSMTF
                     EHHLGRYD"
BASE COUNT          926 a         2435 c         2349 g          960 t
ORIGIN      
        1 gtcgatcgtt ctcggatagg cagtgaaccc ggccaggatg atcttcggcc ggtgctcccg
       61 ggccaggtcg cggacacggt ccatgtcgat cagctccgtc tgcgggtcga cctcgtagcc
      121 gacgaagcgg aaccacttcc cggagacgtt caccggcgag ccgtgggtca ggtggccgcc
      181 gtgggcgagg accagtccga gcaccgtgtc ctcggagtgt ggctggacga acgcgccata
      241 cacggcgagg ttcgcctggg cgccggcgtg gggctgcacg ttggcgtgct cggcgccgaa
      301 cagctgcctg gcccgctcga tggcgagcgt ctcggagacg tcgacgaact cgcagccgcc
      361 gtagtagcgc ctgcccggat agccctcggc gtacttgttg ttcatcgtcg aggagagcgc
      421 cgcgagcacc gcggggctgg cgtagttctc ggacgcgatc agccgcagcg tgcgccgctc
      481 ccggtcgagc tcgttcgcga tcgcctcgaa gacctccgga tccgtcgcgc ggagcgcctc
      541 gaagtccgcc gcccattccc tcgtcatcgg cactcctcct cctcgatcgc ggcgatctgt
      601 tcgaggcggc gcacgtgtcg gccctcctcg aagtcgctgc ggaaccacgt ggtcacgatc
      661 tcgcgcgcga gctccggggc gacgatgcgc gccccgatcg ccagcacgtt ggcgtcgttg
      721 tggcgccggg acatgcgggc ggtgtagagg tcgttgcaca gggcggcgcg tacgccgcgc
      781 accttgttcg cggcgatctg ctcgccctgg ccggagcccc cgagcatgat cccgcgctcg
      841 gcgcggccgg cggccacctc ccgcccgacc gccgcgcaga tcggcgggta gtccacgggc
      901 tcctcggagt cggtcccgag gtcggccacc tcgtacccct cctccttcag gaacgccttc
      961 agctcctcct tcagtcggta cccggcgtga tcggatccga tcgcgacccg gaacccggtc
     1021 atctcactcg tcttcccaca tcgaggtccg ggccggttga cggccggcga tgccctcgat
     1081 cagctggccg agcagattgt cgagctgcca ggccgtcgcg cgataggtct cgaacggcat
     1141 gccgaggggg tcggcgatgt ccaggtcgta cggcggcggg ggcgcgacgc ggcggcgcgc
     1201 ggcggcctcc gcgacgcggt cccggaaccg cccgtcgtcg agcgcccggg gatcgtcgag
     1261 cggcgggagc gctttcagga tctccacgag ctcgcgcagc gtgaagagct tcccggccgc
     1321 atccggaacc atctcgtaga cggcctcgac gtgctcgacc gccatgccga tcaccaggtc
     1381 ggccgcttcg acgtgctccg ggcgcaggcg tcgggcgcgg tgctcggaga tgtcggcctc
     1441 ccgctcctcg gcggcgcgga cggcctcgag cattgcccga tggccgtcga cggcgatcag
     1501 cccggccgag gagacctcga agggcttctc cggaaagcgg tgccgcaggt gctcccgcaa
     1561 caggccctcg gccatcggcg aacggcagat gttgcccgtg cagacgaaga ggaccgacgg
     1621 catggcgccg agtctagcgc gtcgctctcc ccccctcggg ggtcccctgc agggcggtca
     1681 ccatcagctc cgccaggtcg atcggaccct cgcgcaggac gagtgccggg ccgacgagcg
     1741 agacgagggc ggagggcgtt ccgagcggcg ccggaccggc gtccaggtac acgccggcgc
     1801 ggtcgccgag cacggcttcg atctccggga cggtcgccgg cgtcgattcg ccggagcggt
     1861 tcgcgctggt caccgccagc ggcccggtcc gggtgaggag cgcgagcgcg accggatggt
     1921 cgggcatccg cacggcgagg gtcgcgcgcg cctcgcccag ctcccagtcg acccccggtc
     1981 tccgcggcag gacgatcgtg agcgggcccg gccagtagcg ctcgatcagc cgccgggcgc
     2041 ggtcgtccac ctccaccagg cggtcgacgt gggacatgct cgccacgagc accgggatcg
     2101 acagcgagcg cggccgcccc ttcgcctcga agagcctgga tacggccgcc ggcagcgccg
     2161 ggtcggcagc aagcccgtac accgagtccg tcggcatcac gaccagctgc cccgccgcga
     2221 gcgccgcggc ggcacgcgcg atgtcgtcgg ggtcgggatc ggccgggtcg accaccagta
     2281 cggtcacgaa ccggtgcggg cgccagcgac gcggtcgcgg agggcgaggt cgggaaggat
     2341 ctcgatctgc gcgtagcccg tgtccgcgag gatcgagcgg acctcctccg cctgcgacgc
     2401 gccgatctcc atcaccaggg cgccacccgg cagcagccag tcgcgcgccg cggccgccag
     2461 ccggcggtgc acctcggtcc cgccgaccag cgcctcgcgc gggtcggcac ggacctcggg
     2521 tggcaggtcc gcatactccg ccgcggacac gtaggggggg ttcgagacga ccagctcgac
     2581 ccggccgcgc agggcggccg gaagcggctc gagcaggtcg ccctcacgca cgacgacggc
     2641 cagcccgtgg gactcggcgt tgcgtcgcgc cagcgcgacg gccgccgggt tcaggtccgt
     2701 ggcgtggacg atcgcgccgg ggtgctcggc ggcgaccgac agcgcgatcg cgccggtccc
     2761 ggtcccggca tcgaccacca gtggagcatc gatcccggcg atcgcatcga gcgccgcctc
     2821 gacgaccact tcggtctccg gccggggaac gaagacgccg ggctcgacgg ccaggcgcag
     2881 cgtgcggaac ggctgcgcgc cggtgaggtg ctgcagcggc gtgcccgagc agcgctggca
     2941 gagcgcccgg ccgaacgcct tcgccgatgc catgtccatc gcggtccgcg tgtagatctc
     3001 cgcccggtcc gttcccagca cccgcatcag gagcgcctcg gcggtctcgc gcggtgcctc
     3061 cacgccgtgg cgctccaggt agtcggcggc gcggttcaga acctcgacgg gccgcgcgcg
     3121 catcgccgtc agccctctcc gttctgccgc ccgagctgct cggcgcgctc gttcgccatc
     3181 aacgcgtcga cgaacgggtc gaggttcccc gccagcacgt ccggcaggtt gtggacggtc
     3241 aggccgatgc gatggtcggt gacccggttc tgcgggaagt tgtaggtgcg gatcttctcg
     3301 gagcgctcgc cggtcccgac ctgggcgctg cgcgcggccg cctccttcgc ggccgcctcc
     3361 cgctgcgcca gctgcagcag ccgcgcgcgc aggtagcgca tcgccttgtc ccggttctgc
     3421 atctgggagc gctcctcctg aacgctgacc acgatgccgg tcggtctgtg cgtgacccgc
     3481 accgccgagt cggtggtgtt cacgctctgg ccgccggggc ccgaggaacg gaacacgtcg
     3541 atctggagct cgtcgcgctt gatgtccacc tccgtctcct ccgcctccgg cagcaccgcg
     3601 acggtggcgg tggacgtgtg gatccggccg ccggactcgg tcaccggcac gcgctggacg
     3661 cggtgcacgc cggcctcgtg cttcatccgg ctgaaggcgc ggtccccgcg cgctgcgagg
     3721 acgacctcct tcaccccgcc gaggtccgac gggttcgacg agagcacctc ggtcttccag
     3781 cggtgccgct cggcgaagcg gcggtacatc tcgagcagct cgccggcgaa cagcgccgcc
     3841 tcgtcgcccc cggtcccggc gcggatctcg atgatcacgt tccggtcgtc gttcgggtcc
     3901 ttcggaagca gcaggatctc gagctcccgc tccagctcgg ccgcccgcgc ggccgcctgg
     3961 tccgcttccg ttcggaagta ctccgccata tccgggtcgg cctcgccctt ggccagggcc
     4021 agcccctcct cggcctgggc ggccgccccc cgatacgcgc ggaaggcccg cacgaccggc
     4081 tcgagctcgg cgtgctgctt cccgaggccg cggagcttcg ccgggtcggc cgccgtctcc
     4141 ggcaggctca gctccgcccc cagctcctcg aaccgccgct cgatcccctc gagccgctcc
     4201 ctgacctgcg cgttcataca cccattatcg gtgccagcac acagacctca ggtcctgcgc
     4261 gccgagccag cgactggctc tgcgcccgct acggcctggc ccggcacggc ccgggagtgg
     4321 gtgcgggcgg cgcaccggtt ggccgacgcc ctcgtgcggt tggcgacggc cgcgggaggg
     4381 ggaaccgaac ccggtcaccc tcgtgatcca cgccgacgcc tcggtgctcg ccgggcggcg
     4441 cccgcggagg gcccggtgct ttccgagaga ggacgggcag cgcctcgcgg tcgggcgagc
     4501 gcccgacgag cggtacctgt ctgaaggcta ggagtcggag gtcgcgggct cctgcgcagc
     4561 gccggtctcg tcgagcgtcc gcggcgggag cttgccctct gcgcggagga cctcctggaa
     4621 cgaggcgatg gcgacctcga tctggtcatc ggagggaggc ctggtggtga tcttctgcag
     4681 ccacaggccg ggggccatca gaaagcgcat cacggggctg tgcgtgtact tcgcgccgag
     4741 gcggagcagt tcgtaggcga tgccggcgat caccgggatc gccacgatcc gggacaggat
     4801 cgcccagagg atcggcgggt tcccgaacag cgcgaacacc aggatcgtca ggatcatcac
     4861 gatcagcagg aagttcgtgc cgcagcggac gtgcagcgtc gagaaccggt ccacccgctc
     4921 gggaacgagc ggctcgccgt gttcgaacgc agcgatcgtc ttgtgctcgg cgccgtggta
     4981 ctggaacacc cgccggatgt ccttcatccg tccgatcagc gccaggtatc cgaggaacag
     5041 cgccacccgg aacaggccct cgagcacgtt gcgcaggatg ctcgagtcga cggtcctgcg
     5101 gaagaagctg aagagcaccg ccggcccgac cacgaacagc ccgatgaaca gcacgagcgc
     5161 gagcgtcatc gagaaggcca tctggcccct ggtcagcttg gcctcctcct cggcggactg
     5221 gttggccgaa gccgtcagcg ccttgacccc gatcgcgagc gcctgcccga gcccgatcac
     5281 cccccggaac cccggccagc gcaggacgcg gtagcggttc gccaccgagt cgatgtggtg
     5341 cgcttccagg tggatggacg tgtcgggcct gcgcacggcg agggcccagt ggtccttgcc
     5401 gcgcatcatc acgccctcga gcaccgcctg gccgccatag aagtgcggcg gctggggctc
     5461 gggccctacc gggtcgagcg cggggccggt ctcggccgcc acggcccgat cagccgcgct
     5521 tggcgtagcg acgctggaag cgctcgacgc gcccgccggt gtccaccagc ttctgcttgc
     5581 ccgtgtagaa cgggtggcag ttcgagcaga tctcgacgcg gatctcgggc ttcgtggagc
     5641 gcgtgacgaa cgtgttcccg cacgagcagt gcacggtcgc gacgacgtat tcggggtgga
     5701 ttccctgctt catctcactc ggttcaacga cgggctattc ccgccccagg gtagcaggta
     5761 cgatccggcg gttccgatcc gacagccctg agaggccccg acaggaggta gccccacgat
     5821 gagcagcgac gagtccctcc cccacccctc gctcggcgtc tccgaggagg aggcccaggc
     5881 acgcttcgag gccctccagg cgaagctcgt gccgctctgg aggtcgatcg cggcgctgaa
     5941 ccaggacccg cagacgatcg tggtcgtccc ctccctgacc ctcgaccagc tcgaggagca
     6001 cggcgtggag atgcaggcct acgaggagcg ctacctgttc ctgctgttcc tgctccgcca
     6061 gccgcgggcg cggatggtgt acgtcacctc gcagaagatc gcgccgaaca tcctcgacta
     6121 ctacttccac ctgctgcccg gggtcatctc cagccacgcc cgcaagcggt tcttcaaccc
     6181 ggccccccag gacggggcgg cccgggcgct ctcgcagaaa ctgctggagc ggccccggct
     6241 gctggaggag atccggggcc tgatcctcga cccggaccgg gcgcacatgg tgccctacaa
     6301 caccacgacc ctcgagcgcg acgtcgccct gcggctgggc atcccgatgt acgcggccga
     6361 cccgaagttc tttccgctcg ggacgaagtc gggctgccga aagctgttcg ccgaggaagg
     6421 ggtcccgcac ccgctcggcc acgaggacct gcgcggcgag gacgacttgg tcgacgcgct
     6481 ggtcaagatg cgcgccgagc ggccggccat gcagcgtgcc atcgtcaaga cgaacgaggg
     6541 ggtctcgggg ggcggcaacg cccacgtcga cctcgccggc acgccggcgc ccggggaccc
     6601 cgccgagcgc gacgcggtgg tggaccgggt gcgctcgatg acgttcgagc accacctggg
     6661 acgctacgac
//
LOCUS       DASPWO010000500         5148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1091729, whole genome shotgun sequence.
ACCESSION   DASPWO010000500 DASPWO010000000
VERSION     DASPWO010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091729"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1759)
                     /locus_tag="VEO00_11475"
     CDS             complement(<1..1759)
                     /locus_tag="VEO00_11475"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HYU58656.1"
                     /translation="MSITAYLLGPPVLIRDDVVYPAPRGRKVWALLAYLALSDRQPSR
                     QQLIDLLFPDAEDPAGALRWNLSQLRRLLGGPDTIGSGSVVQLRLPEGSVIDVHVLTA
                     GTSHAAVELPGLGRELLEGLDVEASAGFSAWLLGERRRLLALSMAVLREGAFRALASG
                     NARRAVELATRLVAADPLDEDAHALLIRAFAGTGDGVAVERQLAASADLFRRELGTEP
                     GPGLAEAARIPIPSAAPVVGGGRTALLTLLESGEAAVAAGAIEAGLEKLRGAVAGTRE
                     SGETALEAAACLALGSALVHATQGKDEEGSAALHRSIATAEATGERAIAASAHRELGY
                     VELLRGDYARATIWLQTAEQLAAGDPAELARIQAARGAGLADTGVHDRAADAFLDSIR
                     LAETTGQVKQQAWATAVLGRTHLLRDELDRAEELLERGRTLARGERWTAFLAYPEALL
                     AEVWVRQGDLERADQAFEHAFALGCQVDDACWEAYGVRGFGLLRAARGDLAGSVDVMQ
                     EALARCARQRDTHLWLRAYVLDGLCAVAIAARHPMASRWVTDLASLAGRSGMRELSVR
                     AYLYRRDLGDGSAIAAARVL"
     gene            1884..3254
                     /locus_tag="VEO00_11480"
     CDS             1884..3254
                     /locus_tag="VEO00_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYU58657.1"
                     /translation="MKTLAIDELRERVRGNVIIPEDEGYDEARRVYNGMIDRHPAVVV
                     RAVNVGDVIAGVNFARENQLDLAVRGGGHSVPGFGTCDDGLVIDLSPMRGVRVDPATA
                     TARAEGGATWGDFNAAAYPFGLATTGGIISTTGVGGLTLGGGIGYLTRGRGLSIDNLL
                     SVDVVTADGRFLVASEREHEDLFWALRGGGGNFGVVTSFEFRLHPVKDIYGGPMFYEL
                     TEVGNILRFYRDYIAEAPEQIGAFPAFQIAPPLPFIPEDRHGDTFIAMVACWAGPLEE
                     GERALRPFHDVAPVVAEHVGPMPYPALNSAFDPLLPPGLQHYWKASFVTELTDEAIAA
                     HAVHGPGVPAVNSTMHIYPINGAAHRTAADATAFAYRDANFATVIAGVWPDPGRNEAN
                     IEWVRGYYEAIAPHSEEGGYINFMAGDDQDRIRVNYRGNYARLVEVKRMYDPDNLFHL
                     NQNIRP"
     gene            3702..4349
                     /locus_tag="VEO00_11485"
     CDS             3702..4349
                     /locus_tag="VEO00_11485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYU58658.1"
                     /translation="MNHSNRFHPSSRPAGHGITIGTPRLYDLSAALLFVGRRRAFGRL
                     LAAAGVQRGDRALEVGCGTGYFARMLADRVGPEGAVVGIDAAPEMTTYASRKARRLSN
                     CRFQPGTAESLAFPDAAFDVVVSSLMVHHLPEDTRLRAVREMRRVLRPGGGLLLADFR
                     LPERGTWRLVASLHGVVAMQRRVPPLEPLVAEAGFTGLRSGNVPPWLRYVSATNP"
     gene            complement(4420..>5148)
                     /locus_tag="VEO00_11490"
     CDS             complement(4420..>5148)
                     /locus_tag="VEO00_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58659.1"
                     /translation="AIFAAAAAGGLYGIGLVIGWFDNELGGIHRVHDLGSGVLYGVIL
                     TVAFVAMTWRPEWKTSAFFQVVATAVAVLIAGLVSGEGGYVVFGLLVMAVAGILLALH
                     PARTEVLRPALRPSPVMGALALAGSVPLVWFGLTMARLETGLSTDPHEGHWATMAAMA
                     FGLVLTGLLASSRMRGWRLTAWCAGLGAAVYGLASIVFHRFPGTDVPYPGSEGFGWGL
                     AALIGGLVFVAVAEWESRRPQRVG"
BASE COUNT          774 a         1926 c         1769 g          679 t
ORIGIN      
        1 ccagcacgcg tgccgcggcg atcgccgacc catcgccgag atcccggcgg tacaggtagg
       61 cccgaacgga cagctcgcgc atccccgaac ggccggcgag cgatgccaga tccgtgaccc
      121 accggctggc catcggatgg cgcgccgcga tggccacggc gcagagtccg tcgaggacgt
      181 aggcgcgcag ccacaggtgg gtgtcccgct ggcgggcaca gcgagccagg gcctcctgca
      241 tcacgtcgac cgatccggcg aggtcgccgc gggccgcccg cagcagcccg aagcctcgca
      301 ccccgtaggc ctcccagcac gcgtcgtcca cctgacagcc gagcgcgaac gcgtgctcga
      361 acgcctgatc cgctcgttcc aggtcgccct gccggaccca gacctcggcc agcagcgcct
      421 ccggataggc gaggaacgcc gtccaccgtt cgcctctggc caacgtacgc ccacgctcca
      481 gcagctcctc ggcgcgatcg agctcgtccc gcaggaggtg cgtgcggccg aggaccgccg
      541 ttgcccaggc ctgctgcttc acctgtccgg tcgtctcggc gagccggatc gaatcgagga
      601 acgcatcggc cgcccggtcg tgcactcccg tgtcggccag gccggccccc ctggcggcct
      661 ggatcctcgc gagctcggcg gggtcgccgg cggcgagctg ctcggcggtc tgcagccaga
      721 tcgtggctcg ggcgtagtcg ccgcggagca gctccacgta gcccagctcc cggtgggccg
      781 acgcggcgat ggcccgctct cccgtcgcct ctgcagtggc gatgcttcgg tgaagcgccg
      841 cggagccctc ctcgtccttg ccctgcgtgg catgcaccag cgccgagccg agcgccaggc
      901 aggccgccgc ctcgagggcg gtctcgccgg attcgcgggt cccggccacg gcgccccgca
      961 gcttctcgag gcccgcctcg atcgcgccgg cggcgacggc ggcctccccg gactcgagga
     1021 gcgtcagcag cgcggttcgc cccccgccca cgacgggggc cgcgctcggg atcgggatcc
     1081 gggcagcctc cgcaagcccg gggccgggct cggtgcccag ctctcgccgg aacaggtcgg
     1141 ccgacgcggc gagctgccgc tcgaccgcca ccccgtcgcc ggttccggcg aaggcccgga
     1201 tcaacagggc atgggcgtcc tcgtccagcg gatcggccgc caccagccgg gtggcgagct
     1261 cgaccgccct ccgcgcgttc cccgaggcga gcgcccggaa ggcaccctcc cggaggacgg
     1321 ccatgctcag ggccagcagg cggcgccgct cgccgagcag ccaggccgag aacccggcgc
     1381 tcgcctcgac gtccaggcct tcgagaagct ccctgcccag cccggggagc tcgacggccg
     1441 cgtgcgacgt cccggccgtc agcacgtgca cgtcgatgac ggagccctcc ggcagccgga
     1501 gctgcacgac gctcccgctc ccgatcgtgt ccggcccacc gagcagacgg cgcagctggg
     1561 acaggttcca gcgcagggcg ccggcgggat cctccgcgtc ggggaacagg aggtcgatga
     1621 gctgctgccg gctgggctgc cggtccgaga gcgcgaggta ggcgaggagg gcccacacct
     1681 tccggcccct cggcgcgggg tagaccacgt cgtcgcggat cagcacgggc ggcccgagca
     1741 ggtaggccgt gatcgacatg ggaccccatc ctgggccgga tccatcggaa cggcaacggc
     1801 gagcacgatc gccgcctcac acgctccgtc acacggcgcg gccgtacggt ccccgccgga
     1861 ccgaggagcg agggaggcct ggcatgaaga cgctcgcgat cgacgagctg cgggagcgcg
     1921 tgcgcgggaa cgtgatcatc cccgaggacg agggctacga cgaggcccgg cgcgtctaca
     1981 acggcatgat cgaccggcac cccgccgtgg tcgtgcgagc cgtgaacgtc ggcgacgtca
     2041 ttgccggagt gaacttcgcc cgcgagaatc agctcgacct ggcggtacgc ggtggtggtc
     2101 acagcgtgcc ggggttcggc acgtgcgacg acggtctcgt gatcgacctg tctccgatgc
     2161 gcggcgtgcg ggtggatccg gccacggcga cggcccgagc cgagggcggc gccacctggg
     2221 gcgatttcaa cgccgccgcc taccccttcg gcctggcgac caccgggggc atcatctcga
     2281 ccaccggggt gggcggtctc acgctgggtg gcggtatcgg ctacctgacc cgcgggcgcg
     2341 gtctctcgat cgacaacctg ctttcggtgg acgtcgtcac ggccgacggg cggttcctgg
     2401 tggccagcga gcgcgagcac gaggacctgt tctgggcgct ccgcggcggc ggcgggaact
     2461 tcggggtggt gacgtcgttc gagttccggc tccatcccgt gaaggacatc tacggaggcc
     2521 cgatgttcta cgagctgacc gaggtcggga acatcctgcg gttctaccgc gactacatcg
     2581 ccgaggcccc cgagcagatc ggggcgttcc ccgcgttcca gatcgccccg ccccttccgt
     2641 tcatcccgga ggaccgccac ggcgacacgt tcatcgcgat ggtggcctgc tgggccgggc
     2701 cgctggagga aggcgagcga gcactgcggc cgttccacga cgtggcgccg gtggtcgcgg
     2761 agcacgtggg cccgatgccg tatcccgcgc tgaacagtgc gttcgacccc ctgttgcccc
     2821 ccggcctcca gcactactgg aaggccagct tcgtgaccga gctgaccgac gaggcgatcg
     2881 ccgcccacgc ggtccacggg ccgggcgtgc ccgccgtcaa ctcaacgatg cacatctatc
     2941 ccatcaacgg cgccgcccac cggacggcgg ccgacgccac ggcgttcgcg taccgcgacg
     3001 cgaacttcgc cacggtgatc gccggggtat ggcccgaccc cggccggaac gaggccaaca
     3061 tcgagtgggt gcgcggctac tatgaggcga tcgcgcccca ctccgaggag ggtggctaca
     3121 tcaatttcat ggccggcgac gaccaggacc ggatcagggt caactacagg gggaactacg
     3181 cgcggctggt cgaggtcaag cggatgtacg acccggacaa cctcttccac ctgaaccaga
     3241 acatcaggcc ctagggcctg acccgaggcc ggcggttctc acggagtccc tcgtcgccgc
     3301 cacggcagga cgatgtccac gcgacggagg gggaccccgg ccttccctgc atccgagcgc
     3361 accgtgagca cctggtgaac ctggggggct ccctcctcga accggcgcgc cgaccccgcc
     3421 gtgaacagcc gccatacccg ggaaccgtcc cgtcccggga gggcccgagc atcaagcggt
     3481 ggaagtcgtt cgagatgtcg tagtgatggg cgacggcacg ggcgtcgcgc tcccgggagg
     3541 gtggcaacgt ccatcgtctc tctccgtcag gacctgtaca tcacgacctt cctctcccca
     3601 accttcaccc gcccttgaac agccgccccg gcagcgagac cggcgcgatc ggccggcgca
     3661 tgcgtcaatg gccacgcccg tcttcagcga gaggaggagc cgtgaaccac tcgaaccgct
     3721 tccatccatc ctcccggccc gccggccacg ggatcaccat cggcacgccg cggctctacg
     3781 acctgtccgc ggcgctgctc ttcgtcggcc ggcgccgcgc gttcggcagg ctgctcgcag
     3841 ccgccggtgt ccagcgcggc gatcgtgcgc tcgaggtcgg ctgcggcacc ggctacttcg
     3901 cgcggatgct ggccgatcgg gtcgggccag aaggcgccgt cgtcggcatc gacgcggctc
     3961 cggagatgac cacctacgcc agccggaagg cccgtcgcct ctcgaactgc aggttccagc
     4021 caggaacggc cgagtcgctc gccttccccg acgccgcctt cgacgtggtc gtgtccagcc
     4081 tcatggtgca ccatcttccc gaggacacac gactccgggc ggttcgggag atgcggcgcg
     4141 tgctccgccc cggcggcggg ctgctgctgg ccgacttccg gttgccggag cgcggtacgt
     4201 ggcgcctggt cgcctcgctc cacggcgtcg tggcaatgca gcgccgggtc ccgccgctcg
     4261 agccactggt cgcggaggca ggcttcaccg gtctgcgctc cggcaacgta ccaccgtggc
     4321 tccgctacgt gagtgcgacg aacccctgat cggccatgaa gatgcccctc ccgccagtcc
     4381 tgcagggtcg atgctcccag cgggtgccag gcgttgccgt cacccgaccc gctgtggtcg
     4441 ccgcgactcc cattccgcga ctgccacgaa cacgaggccg ccgatcagcg ccgccagacc
     4501 ccagccgaac ccttcgctcc cggggtacgg gacgtccgtg cccgggaagc gatggaagac
     4561 gatcgacgcg agcccgtaga cagccgctcc gaggccggca caccatgccg tgaggcgcca
     4621 cccgcgcatg cgggacgagg cgaggagccc cgtgagcacc agcccgaagg ccatggcggc
     4681 catggttgcc cagtgaccct cgtggggatc ggtggataga ccggtctcca acctggccat
     4741 cgtcagaccg aaccacacca ggggtacgga tccggccagg gcgagcgcac ccatgacggg
     4801 gctgggcctc agcgccggac ggagcacctc ggtccgcgcc gggtggagcg ccagcaggat
     4861 tccggccacc gccatcacca ggagcccgaa gaccacatag ccgccttcgc cggagaccag
     4921 cccggcgatc agcacggcaa ccgccgtcgc gacgacctgg aagaaggccg aggtcttcca
     4981 ctccggtcgc cacgtcatgg ccacgaacgc caccgtcagg atgacgccgt agaggacccc
     5041 ggacccgagg tcgtggacgc gatggatccc tccaagctcg ttgtcgaacc atccgatgac
     5101 gagcccgatc ccgtacagcc ccccggccgc cgcggccgcg aagatcgc
//
LOCUS       DASPWO010000501         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_582747, whole genome shotgun sequence.
ACCESSION   DASPWO010000501 DASPWO010000000
VERSION     DASPWO010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582747"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VEO00_11495"
     CDS             <1..472
                     /locus_tag="VEO00_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HYU58660.1"
                     /translation="LENALLKARGVAAATGVPALADDSGIEVDALGGRPGPRSARYAG
                     ESATDEKNLSALLRALAGVPSGGRTARYRAVAALAWPDGRVVHGEGTCEGLLIARPRG
                     TGGFGYDPAFVPEGWDRTMAELGPDEKNRISHRARALRALRALLANDRIVTDGA"
     gene            complement(469..>3013)
                     /locus_tag="VEO00_11500"
     CDS             complement(469..>3013)
                     /locus_tag="VEO00_11500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYU58661.1"
                     /translation="RAGRDRSRGRLVVALGLIGLVSILVRVADALGDPLGLVSYAAIV
                     GLLGSGYALLLFRHSFVPLSPGVRRAALAACAATFALAVASNLPVGPDRGFTPLRSAA
                     VVLFALVWAAMLAEPIVALWRAARDRPAVQRARLRTLSAGYAGIMLAVAIGLLSAPTG
                     RHRWIEVAVPLIGLTVVPLLYVAFLPPRWLRRAWRQIEEEALTSSSDAVLLAPDRATL
                     ATRAVEWSMRLLGADGGLLVDRRGNELARIGSVPAEASRAARRGAAAGPWLFRLEGAP
                     ARHAAVVPMHFDEGVGALVLVSGPLTPLFGSDELGRLSHYAASIATALDRARLEGALS
                     RETKHTQVMLQAMSDLGEGYVITRDGRAIDVNRAYEEITGYGRDELVGMSLVDLAVPE
                     DRPALADRGRHRLSGRPEPERYETRLMRKDGTVIDVEVAVKLLGHDGAGGVQMASLVR
                     DITERKRAEAEVRASRELLVDAQRVAHLGSFEWEVDSDRLTWSEEMHRIFGVDPASFT
                     ASLQGYLQRVHPDDRDRVAGAIRRALEGGRPFVFDHRIVRGDGAVRSLHCRGQVEAGA
                     DGRAARMFGTAQDVTERVRAEEALRAAYERERQAADELRALNELKNEFLTAVSHELRT
                     PLTAVRGFANTLEGAEDRLSEEQRHTLLGRLSANAVRLDRLLSDILDLDRLRRGMVRA
                     HRHPTDLPALVRRVVTESDATDGRTVNVAAEPCVIAVDPAKVERIVENLLSNAARHTP
                     ADAVIWVGVRAEGDGALLVVEDAGPGVPPELREALFEPFRQGPTESGRAQGVGIGLSL
                     VARFAELHGGRAWVEERRGGGASFRVHLPDGPAEELPATTEPAGASRSA"
BASE COUNT          394 a         1147 c         1126 g          346 t
ORIGIN      
        1 cctggagaac gcgctgctga aggcgcgcgg ggtggccgcc gcgacagggg tcccggcgct
       61 ggccgacgat tccgggatcg aggtcgacgc gctcggaggg aggcccgggc cgcgatcggc
      121 ccgctacgcc ggcgagagcg ccaccgacga gaagaacctg agcgcgctgc tccgggccct
      181 ggcgggggtc ccgtccggcg gccgtaccgc ccgctaccgg gccgttgcgg ccctcgcctg
      241 gccggacggc cgcgtggtcc acggcgaggg gacgtgcgag ggcctgctga tcgcccgccc
      301 tcgcggcacc ggcggcttcg gctacgaccc ggcgttcgtc cccgagggct gggaccgcac
      361 gatggccgag ctcggccccg acgagaagaa ccgcatcagc caccgcgcgc gagccctccg
      421 cgccctccgc gccctgctgg cgaacgaccg gatcgtgacc gacggggcct aggcggaccg
      481 gctcgcgccg gcgggttcgg tggttgcggg cagctcctct gccgggccgt cggggaggtg
      541 aacgcggaag gaggcaccgc ctccccggcg ctcctcgacc caggcccggc cgccgtgcag
      601 ctcggcgaac cgcgccacga gcgacagccc gatcccgacc ccctgggccc ggccgctctc
      661 cgtcgggccc tggcggaacg gctcgaacag cgcctcgcgc agctcgggcg ggactcctgg
      721 gcccgcgtcc tcgaccacca gcagggcgcc gtccccctcg gcgcggacgc cgacccagat
      781 cacggcgtcc gccggcgtgt gccgggcggc gttggacagc aggttctcga cgatgcgctc
      841 gaccttggcc gggtccacgg cgatcacgca cggctcggcg gcgacgttca ccgtgcgccc
      901 gtcggtggcg tcgctctcgg tcacgacgcg ccgtaccagc gcgggcaggt cggtggggtg
      961 acggtgcgcc cggaccatcc ctcgccgcag gcggtccagg tccaggatgt cggagagcag
     1021 ccggtcgagc cgaaccgcgt tcgcggagag gcggccgagc agcgtgtggc gctgctcctc
     1081 cgacagccgg tcctcggccc cctcgagcgt gttggcgaac ccgcgcaccg ccgtcagcgg
     1141 cgtgcgcagc tcgtgggaga cggctgtcag gaactcgttc ttcagttcgt tgagggcccg
     1201 cagctcgtcg gccgcctggc gctcgcgctc gtaggccgcc cgcagggcct cctcggcgcg
     1261 tacgcgctcg gtgacgtcct gggccgtgcc gaacatccgc gcggcccggc cgtcggcccc
     1321 ggcctccacc tgcccgcggc agtgcagcga gcggacggcg ccatcgccgc ggacgatacg
     1381 gtggtcgaac acgaagggcc gcccgccctc gagggcgcgg cggatggccc cggcgacccg
     1441 gtcccggtcg tccgggtgta cccgctgcag gtacccctgg aggctcgccg tgaacgacgc
     1501 cgggtcgacc ccgaagatcc ggtgcatctc ctccgaccag gtgagccggt ccgagtcgac
     1561 ctcccactcg aagctcccga ggtgcgccac gcgctgcgcg tcgaccagca gctcccggct
     1621 ggcgcgcacc tccgcctcgg cccgcttgcg ctccgtgatg tcgcgaacca gtgaggccat
     1681 ctgcacgccg ccagccccgt cgtgcccgag cagcttcacc gcgacctcca cgtcgatcac
     1741 ggttccgtcc ttgcgcatca ggcgcgtctc gtagcgctcg ggctccggcc ggccgctcag
     1801 ccggtggcgg ccgcggtcgg ccagcgccgg gcgatcctcg gggacggcca ggtccaccag
     1861 cgacatgccg accagctcgt cgcgcccgta gccggtgatc tcctcgtagg ctcggttgac
     1921 gtcgatcgcg cggccgtcgc gcgtgatcac gtagccctcc ccgagatcgc tcatcgcctg
     1981 cagcatcacc tgggtgtgtt tcgtctcccg ggagagcgcg ccctccaggc gcgcgcggtc
     2041 gagcgcggtt gcgatcgagg ccgcgtagtg cgagagccgg ccgagctcgt cggacccgaa
     2101 cagcggggtg agcgggcccg agaccagcac gagggcgccg acgccctcgt cgaagtgcat
     2161 cgggaccacg gcggcgtggc gggccggcgc gccctcgagg cggaacagcc acggcccggc
     2221 cgccgcgccg cgccgcgccg cgcgcgaggc ctccgccggg acgctcccga tccgcgccag
     2281 ctcgttgccc cgccggtcca ccaggagccc gccgtcggcc ccgagcagtc gcatcgacca
     2341 ctccacggcg cgggtggcca gggtcgcacg gtccggcgcg agcaagaccg cgtcgctgga
     2401 ggaggtcagg gcctcctcct cgatctgtcg ccaggccctg cgcagccagc gcggggggag
     2461 gaaggccacg tagagaaggg gaaccaccgt caggccgatc agcgggacgg cgacctcgat
     2521 ccagcggtgc cggccggtcg gcgccgagag cagcccgatt gccaccgcga gcatgatccc
     2581 cgcgtatccg gctgacagcg tgcggagccg cgctcgttgc acggccgggc ggtcccgggc
     2641 ggcgcgccag agcgccacga tgggctcggc gagcatcgcc gcccagacca gcgcgaacag
     2701 cacgaccgcg gccgagcgca gcggcgtgaa tccgcggtcc gggccaacgg ggaggttcga
     2761 ggcgaccgcg agcgcgaacg tcgcggcgca ggcggcgagg gcggcgcgcc ggacgcccgg
     2821 cgacagcggg acgaaggaat ggcggaagag cagcagcgcg tagcccgacc cgagcagtcc
     2881 gacgatggcg gcgtacgaca ccaggccgag ggggtcgccg agtgcgtcgg cgacgcgtac
     2941 gagtatcgag acgagcccga tcaggccgag ggcgacgacc agtcggccgc gcgaccgatc
     3001 gcggccggcg cgc
//
LOCUS       DASPWO010000502         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_663960, whole genome shotgun sequence.
ACCESSION   DASPWO010000502 DASPWO010000000
VERSION     DASPWO010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663960"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1873)
                     /gene="purL"
                     /locus_tag="VEO00_11505"
     CDS             complement(<1..1873)
                     /gene="purL"
                     /locus_tag="VEO00_11505"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HYU58662.1"
                     /translation="MTGGFGEVARPVERETRPRPASQPPKGNAPLHRALALSDDEFAR
                     IVETLGREPNHAELAMFSAMWSEHCSYKSSRVHLKRLPTEGPQVLVGPGEDAGAVDLG
                     EGVAVVFKIESHNHPSAVEPVQGAATGVGGIVRDVLSMGARPVAILDSLRFGPPDDAR
                     NRYLFGGVVAGIGQYGNSIGVPTVGGEVAFASGYGPNPLVNALCVGIVPSDRLLRARA
                     EGAGNLLVLIGARTGRDGIGGVSVLASRTLEDADRRPSVQIGDPFAEKLLIEACLELT
                     DAGLLVGLQDLGGAGLTCAVSESAARANAGAEVDLDAVPLREPGMEAFEILTSESQER
                     MLAVVRPEDRGAVEGVCGRWGLASAVVGKVTTDGRVRVRMRGEVVAEVPARALTDEAP
                     EYRRPMAPPAWLDALREDDPTFVEPPTDLGDAFLAVLGSPNVASKRWVFEQYDSLVQH
                     NTVRGPGGDAAVIRLEERLKAFAVTTDGNGRLGYLDPYLGAAHAVAEAARNLACVGAR
                     PLAVTNCLNFGNPERPEVMWQFAEAVKGMGDACRALGTPVTGGNVSFYNEAGDSAIHP
                     TPVVGMLGVLEDYRLMVRSAFPASGLSIYLLGRTLPELGGSEFSDVVLGRVSGRPPAL
                     "
     gene            complement(1870..2556)
                     /gene="purQ"
                     /locus_tag="VEO00_11510"
     CDS             complement(1870..2556)
                     /gene="purQ"
                     /locus_tag="VEO00_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006435807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HYU58663.1"
                     /translation="MITFPGSNDDRDAARAIRLLGGEAVPVWHGEADLGGADAVILPG
                     GFSHGDYLRTGAIARFAPALGAVREHAATGRPVLGICNGFQILCEAGLLPGALIRNRS
                     LSFICRVVHVRVETRRTPFTAQLSEGEVLDIPIKHGEGQFVATPGELARIEGEGLAVF
                     RYCTPDGEVSEEANPNGAMDGIAGVRDERGNVVGLMPHPEHAVDPDIGPTGGQPLFES
                     LFAELLATLS"
BASE COUNT          365 a          983 c          891 g          322 t
ORIGIN      
        1 tcagcgccgg cggacggccg gagacacgac ccagcacgac gtcggaaaac tcgctgccgc
       61 cgagctccgg cagggtccgc ccgaggagat agatcgacag cccgctcgcc ggaaacgcgg
      121 aacggaccat cagccggtag tcctccagga ccccgagcat ccccacgacc ggcgtggggt
      181 ggatcgccga gtcgcccgcc tcgttgtaga aggacacgtt cccaccggtg accggcgtgc
      241 cgagcgcgcg gcaggcatcg cccatcccct tcaccgcctc ggcgaactgc cacatcacct
      301 cgggccgctc ggggttgccg aagttcaggc agttcgtcac ggccagcggc cgggcgccca
      361 cgcaggccag gttccgggcc gcctcggcca ccgcgtgggc cgccccgagg taggggtcga
      421 ggtagccgag ccgcccgttc ccgtcggtgg tcaccgcgaa cgccttcagg cgctcctcca
      481 gccggatcac cgccgcgtcc ccgccggggc cgcgcacggt gttgtgctgg accagcgagt
      541 cgtactgctc gaagacccag cgcttggagg cgacgttcgg ggagccgagc accgccagga
      601 aggcgtcacc gaggtccgtg ggcggctcga cgaaggtcgg gtcgtcctcg cgcagcgcgt
      661 cgagccaggc cggcggcgcc atcggccggc ggtactcggg cgcctcgtcg gtcagcgcgc
      721 gggccggcac ctcggccacc acctccccgc gcatccgaac gcggacgcgc ccgtcggtgg
      781 tcaccttccc gaccaccgcc gacgccaggc cccagcgtcc gcagacgccc tcgaccgcgc
      841 cgcggtcctc cggccggacc accgcgagca tccgctcctg cgactccgag gtcaggatct
      901 cgaacgcctc catccccggc tcgcgcaggg gcaccgcgtc gaggtcgacc tccgcgccgg
      961 cgttcgcgcg cgccgccgac tcgctcacgg cgcaggtcag gccggccccg ccgaggtcct
     1021 gcagcccgac gagcagccct gcgtcggtca gttccaggca cgcctcgatc agcagtttct
     1081 cggcgaaggg gtcgccgatc tgaacggaag gtctgcgatc ggcgtcctcc agcgtccggc
     1141 tggcgaggac cgagacgccg ccgatcccgt cgcgcccggt gcgcgcgccg atcaggacca
     1201 gcaggttccc ggcaccctcg gcccgggccc gcagcagacg gtccgacggc acgatgccga
     1261 cgcacagcgc gttgaccagc gggttcggcc cgtaaccgga ggcgaacgcc acctcgcccc
     1321 cgacggtcgg cacgccgatc gagttcccgt actgcccgat gccggccacc acgccaccga
     1381 acaggtagcg gttgcgtgcg tcgtcgggcg gcccgaagcg cagcgagtcc aggatggcca
     1441 ccggacgcgc ccccatcgag agcacgtcgc ggacgatgcc gccgacgccg gtggccgcgc
     1501 cctgcaccgg ctcgaccgcc gaaggatggt tgtggctctc gatcttgaac acgacggcca
     1561 cgccctcgcc gaggtcgacg gcgccggcgt cctcccccgg accgaccagg acctgcgggc
     1621 cctcggtggg cagccgcttc aggtggacgc ggctcgactt gtaggagcag tgctcggacc
     1681 acatcgccga gaacatcgcg agctccgcgt ggttcggctc gcgcccgagg gtctcgacga
     1741 tccgggcgaa ctcgtcgtcg gagagcgcca gcgcccgatg gaggggcgcg ttccccttgg
     1801 gaggttggct cgccggacgc ggtcgagtct cacgctccac agggcgagct acctccccaa
     1861 accccccggt cacgacagcg ttgccaggag ctcggcgaac agcgactcga agagcggctg
     1921 cccgccggtc ggtccgatgt cggggtcgac cgcgtgctcg gggtgtggca tcaggcccac
     1981 gacgttccct cgctcgtcgc gaacgccggc gatgccgtcc atcgccccgt tcgggttcgc
     2041 ttcctcgctc acctcgccgt cgggcgtgca gtagcggaag accgccaggc cctccccctc
     2101 gatccgcgcc agctcgcccg gcgtggccac gaactgcccc tcgccgtgct tgatcgggat
     2161 gtcgagtacc tcgccctcgg acagctgcgc ggtgaacggg gtacgccgcg tctcgacgcg
     2221 cacgtgcacg acgcggcaga tgaacgacag tgagcggttg cggatcagcg cgccggggag
     2281 cagccccgcc tcgcacagga tctggaagcc gttgcagatg ccgagcaccg gccgcccggt
     2341 ggcggcgtgc tcccggacgg ctccgagggc cggcgcgaag cgggcgatcg cgcccgtgcg
     2401 caggtagtcg ccgtgggaga acccaccggg aaggatcacg gcgtcggccc ctccgaggtc
     2461 cgcctcgccg tgccacaccg ggaccgcctc cccgccgagg agccggatcg cgcgggcggc
     2521 gtcgcggtcg tcgttcgagc cggggaacgt gatcacggcc a
//
LOCUS       DASPWO010000503         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_412477, whole genome shotgun sequence.
ACCESSION   DASPWO010000503 DASPWO010000000
VERSION     DASPWO010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412477"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="VEO00_11515"
     CDS             <1..1532
                     /locus_tag="VEO00_11515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HYU58664.1"
                     /translation="LASLAWSILDTNRSPAGAYFSTLARAWELALGAALAIAASRFAT
                     VSDRTRAVMGWAGALCIGVAAVLFSEATPFPGSAALVPTVGTALVIAAGIGNERSPRG
                     VGRLLGRAPLRYVGDRSYAFYLWHWPALVIAAYYVGHEVSVTGNLALLAVAFLVSMAS
                     FRLIENPIRHARLERPIRTSEVLWGTSVGMVGLVALLGIRSIAVEAGSGQAVAPDAVH
                     AFAPLPQPSEPGGPGTAELGDPPAPAVIPAVVVAVEAARLGAPIPSPLLPPVTRLFGD
                     RYDFPRRSCYAEPGETLQQEVCQLVGGASRTIVVFGDSHARQWIPTVSWVASQDGWNL
                     VPLVELGCRPSRYDTACTVYFEWAMEQIKTLHPDVVLIGGQLMVDGPGAMQATVAGIG
                     HVVEAVTPFAKDVIVIGDPPAQSQQPVDCLLARNATMGTCTSTLTKDQVSVYDDVRRA
                     ARRGGAAFVDTMDWFCFEHQCPMVIDRTIAYRDIAHITQTYALQLRELFRAEFARAVS
                     Q"
     gene            complement(1600..2481)
                     /locus_tag="VEO00_11520"
     CDS             complement(1600..2481)
                     /locus_tag="VEO00_11520"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HYU58665.1"
                     /translation="MADWARRAAGPVADAVRGHHPSAIVTSLFGVEVLSEVSPPCPWA
                     VINSTFYVGPDPPRPLEEDFGPRAIPLISRYASLLGSASLVLHATDPSFDLEFDGLPP
                     RHHYVGPLGIWEPPSKSPLYLDEPGDPWVLVTISSQMQDDVALADAALAALSGSPVRG
                     VLTLGPDHDPDELSTIPPNVRVERTISHSAVLGREALLVSHAGHGSVMKALWYGRPMV
                     LVPWGRDQPGVAARAATLGVAEVVPRGEASEKTLSRAIDRALTDGRMKDAAGRHGARL
                     RATDPAAKATGLLEMLL"
BASE COUNT          401 a          983 c          918 g          426 t
ORIGIN      
        1 gcctcgcctc actggcctgg tcgatcctgg acacgaaccg gtcgcccgcg ggcgcctact
       61 tctccacgct ggctcgggcc tgggagctgg ccctgggcgc ggcgctcgcc atcgcggcct
      121 cacggttcgc caccgtttcg gatcggaccc gcgcggtgat gggatgggcc ggagccctct
      181 gcatcggcgt cgcagcggtc ctgttctcgg aggcgacgcc gttcccgggg tcggccgcgc
      241 tcgtgccgac ggtcgggacc gcgctggtca tcgcggccgg catcggcaac gaacggtccc
      301 cgcgaggcgt cggaaggctg ctcgggcgag cgccgcttcg ctacgtcggc gaccgctcct
      361 atgccttcta cctgtggcac tggcccgccc tggtgatcgc ggcctactac gtgggccacg
      421 aggtctcggt caccgggaac ctcgcgctcc tggcggtggc gttcctcgtg tcgatggcga
      481 gcttccggtt gatcgagaac ccgatccggc acgcccggct cgagcggccc atccggacga
      541 gcgaggtgct gtggggaacc tcggtgggca tggtgggcct cgtggccctg ctcggcatcc
      601 ggtccatcgc cgtcgaggcg ggctccggcc aggccgttgc gcccgacgcg gtgcacgcgt
      661 tcgccccgct cccgcagccg tcggagccgg gtgggcccgg aaccgcggag ctcggggatc
      721 caccggcgcc cgccgtgatc cccgccgtgg tcgtcgccgt ggaggcagct cgccttggag
      781 cgccgatccc ctcgcctctc ctgccacccg tcacccggct cttcggcgac cggtacgact
      841 tcccgaggcg aagctgctac gccgaacccg gcgagaccct tcagcaggag gtgtgccaac
      901 tggtcggcgg cgcgtccaga accatcgtcg tgttcggcga ttcgcatgcc cgccagtgga
      961 tccctacggt gtcgtgggtc gcgtcgcagg acggatggaa cctcgttccg ctcgtggagc
     1021 tggggtgccg accatcacgg tatgacaccg cgtgcaccgt ctatttcgaa tgggccatgg
     1081 agcagatcaa gaccctccat ccggacgtcg tcctgatcgg aggccagctc atggtcgacg
     1141 ggccgggagc gatgcaggcg accgtcgccg gcatcggcca cgtggtcgag gcggtcacgc
     1201 ccttcgcgaa ggacgtcatc gtgatcggcg acccgccggc gcagagccag caaccggtcg
     1261 actgccttct ggctcggaac gcgacgatgg gaacgtgcac ctcgaccctc accaaggacc
     1321 aggtctccgt gtacgacgac gtcaggaggg ccgcgcggcg gggcggggcc gccttcgtcg
     1381 acacgatgga ctggttctgc ttcgagcatc agtgcccgat ggtcatcgac cgcacgatcg
     1441 cctatcgcga catcgcccac atcacccaga cctacgccct gcagcttcgc gagctgttcc
     1501 gcgccgagtt cgcgcgcgcc gtctcgcagt agctcgagcg ccgatacggg cgagagaggc
     1561 ctcacactcg gcgccttggt ttcgcaccga tcttttccgt cagaggagca tttcgaggag
     1621 gccagtcgcc ttcgccgctg gatccgtcgc ccgcagtcgc gccccgtggc ggccggccgc
     1681 atccttcatg cgtccgtccg tgagcgctcg atcgatcgcc ctcgagagcg tcttctcgga
     1741 cgcctcgccg cgcggcacga cctcggcgac tccgagcgta gcggcacggg ccgcgacccc
     1801 cggctggtcc cgtccccacg gcacgagcac catgggcctc ccataccaca gagccttcat
     1861 caccgatcca tgcccagcgt ggctcaccag caaggcctcg cgccccaaaa cggcggaatg
     1921 cgagatcgtc cgctctaccc gcacattggg tgggatcgtc gagagctcat ccggatcgtg
     1981 gtccgggccg agtgtgagga ccccccgcac cgggctgccg gacagggccg cgagcgccgc
     2041 gtccgcgagg gccacatcgt cctgcatctg cgagctgatc gtcacaagca cccacgggtc
     2101 gccgggctcg tcgagataca acggcgactt ggacggtggc tcccagatcc cgagtggccc
     2161 gacgtaatgg tgccggggcg gcagaccgtc aaattcgagg tcgaacgatg ggtcggtcgc
     2221 gtgcagcacc aggctcgctg aacccaacag cgacgcgtac cgcgagatca gcgggatggc
     2281 ccgtggtccg aagtcttcct cgagtggcct gggcggatcc ggacccacgt agaaggtgct
     2341 gttgatgacg gcccaaggac agggtggtga gacctcactc aggacttcca ccccgaacag
     2401 cgaagtcacg atcgcactcg gatgatgccc ccgcaccgca tcggcgaccg gcccggcagc
     2461 ccgccgggcc cagtcggcca tgctcctctg gatgagggga cccgcggcga cgagatcgcc
     2521 gcccgttgtc cgcatcgcgt ctcggatggc agcaacgagc gtcggaccca ggtcgagctc
     2581 cgacggcaac gtctccacgt ggaggtccag cccatcaacc gatcgggcca ccgacccgtc
     2641 acccagcacg atgatctcgt gccctcgatg ccggagggcg agagcggccg cgagtagcgg
     2701 ctggagatcg ccgcctgcgc cggcggtc
//
LOCUS       DASPWO010000504         8335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_494653, whole genome shotgun sequence.
ACCESSION   DASPWO010000504 DASPWO010000000
VERSION     DASPWO010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494653"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VEO00_11525"
     CDS             <1..764
                     /locus_tag="VEO00_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU58666.1"
                     /translation="VISNLTVPGDRGTDAVRDLHLEVRSGEIVGIAGVSGNGQRELAE
                     AIAGLRTPTSGSIRLNGVETAGRGPAEIRERGLGYVPEERTRDGMIAGFSVSENLMLV
                     DHRDPRFSRWGFLRPGAIQRHCAELVSAFRVKTPGLSTPGSNLSGGNIQKLVLARELS
                     GSPRVLLVAQPTRGVDVGAAEYIQTRLLEQRGHGTAILIISGDLDEVLALSDRILVMY
                     EGTIIGEVDAQSATREALGLMMAGVRPDAQEEARA"
     gene            complement(791..976)
                     /locus_tag="VEO00_11530"
     CDS             complement(791..976)
                     /locus_tag="VEO00_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58667.1"
                     /translation="MEDRYRQLKQKRDRVGLSHDEANELGRLIAEREGKEYANADMLR
                     PHSAREGISVFARAETR"
     gene            complement(1013..1228)
                     /locus_tag="VEO00_11535"
     CDS             complement(1013..1228)
                     /locus_tag="VEO00_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HYU58668.1"
                     /translation="MDADFRMRMEGRWDQVKGRVKEAWGSLTDDDLDRDEGNWDRLVG
                     TIRERTGESLDAIETKLKDLFDRADTQ"
     gene            1343..1789
                     /locus_tag="VEO00_11540"
     CDS             1343..1789
                     /locus_tag="VEO00_11540"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HYU58669.1"
                     /translation="MDAALEAARVCQAWGDVPIGAVVARDGAVLASAGNQRERAQDPT
                     AHAEILALRDAAAAAGSWRLDGCDVFVTLEPCAMCAGALVLARVDRLVYATPDPKAGF
                     AGSLGDLTRDARLNHRLEVVSGVREAEASELLRAFFRGRRGATEGV"
     gene            1813..1900
                     /locus_tag="VEO00_11545"
     tRNA            1813..1900
                     /locus_tag="VEO00_11545"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1847..1849,aa:Ser,seq:cga)
     gene            1941..2027
                     /locus_tag="VEO00_11550"
     tRNA            1941..2027
                     /locus_tag="VEO00_11550"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1975..1977,aa:Ser,seq:gga)
     gene            2082..2345
                     /gene="ffs"
                     /locus_tag="VEO00_11555"
     ncRNA           2082..2345
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VEO00_11555"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            2388..4088
                     /gene="dnaX"
                     /locus_tag="VEO00_11560"
     CDS             2388..4088
                     /gene="dnaX"
                     /locus_tag="VEO00_11560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HYU58670.1"
                     /translation="MTESKTRTGNGARTPAALRAEPHRSLYRKYRPQGPSEVIGQEHV
                     IRALVGAIREGRLAHAFLFCGPRGTGKTSTARILAKMVNCERGPTPEPCGVCEQCVAI
                     RDGQHLDVVEIDAASHGGVEDARELRERAPTAPAQGREKVYIIDEAQRLSREAFDALL
                     KLFEEPPSGVRFVLATTEPHKMPATIIGRCQRFDFRRIAWDALAGHLAAIAEREGVSL
                     APEAAQTIARHAEGSARDALSLLDQASVLGDGDVDEETVLSLIGAADLEVRFALADAV
                     AAQDPRTVFEIVHRLVQEGFDLRSVTAQVIAHFRDLFLALVAVDQADLLDVPAEHQAR
                     LAAQAAKFTPAELNRILSVLLEANTNLRWTTSPRLTLELALVKAAVPEADAHAEGLLS
                     RIERLERLSGLKDAPPASPESPAPAPAPPPSPAPAEERVTEEPATEPHAPSTGVVDTD
                     LVRRSWPEVLKALDTASNRVLHTVAQLVVMQTLDGDTLELVVPAGKKFAAEKIMQKQQ
                     ELRAVLRDILGITPALRCVVRDGSPATDDPILEDEAPPTPELAIERVMEGLGAEIVED
                     "
     gene            4113..4706
                     /gene="recR"
                     /locus_tag="VEO00_11565"
     CDS             4113..4706
                     /gene="recR"
                     /locus_tag="VEO00_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HYU58671.1"
                     /translation="MFEGPIQNLIDELNRLPGIGPKSAQRLAFYLVKAPAADAKRLAE
                     AIVQAKERVSFCRECFNVAEGALCRFCADPSRDASVICVVEEPKDVAAIEKAGIVRGR
                     YHVLGGAISPMEGIGPDDLRIQELLDRVGRDHTQEVIVATNPNLEGNATAMYVAGLLK
                     PLGVRVTRLASGLPVGGDLEYADEVTLGQALEGRREL"
     gene            complement(4731..5309)
                     /locus_tag="VEO00_11570"
     CDS             complement(4731..5309)
                     /locus_tag="VEO00_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58672.1"
                     /translation="MTARLSPGPGDGSPSRRRVAKTVPAGVALALLLLAACGETATPA
                     ADDTGEGSGCSPWALRPQYLPWSGEGDPGPPDETRDASATWFAGTPGDSGAGYVTIEA
                     SDVALLDPNAAAATPTVRGSASVLVWAGDPGVGELALGWREADAACGHLTLRLGTGFI
                     EPPAGTDGDAAIAAVEEEAIREIQRVADSLQG"
     gene            complement(5306..5668)
                     /locus_tag="VEO00_11575"
     CDS             complement(5306..5668)
                     /locus_tag="VEO00_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58673.1"
                     /translation="MRPRPLPCAGRLPADADGRYPSDLLLAAKRSPAYRRLRCHLDGP
                     CPEDVWADGPRDAFVVFRLRPGTSATHVGFALDRRRREVTRVRLLSIGPWTVAGSEVT
                     LAPCGDRRPAVTDREVSG"
     gene            complement(5675..6526)
                     /locus_tag="VEO00_11580"
     CDS             complement(5675..6526)
                     /locus_tag="VEO00_11580"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYU58674.1"
                     /translation="MGLQARFRETTPTVTRRHFMQMAILATSSVALPLPRLWAAQPRE
                     PGAAGGGEEVRPPRYCTPGATGLDPGAEAGPVRLRRIDAPRNGDWSGVAWPFGGGRGL
                     PGHYQGAPRADWIMVHHTGAPNAHLETDACADCYYYDFCIDRSGDICVNGRWDDSTGA
                     HARGCNCRAVGIMLYGCFGGCNGGNVSAPSNAQLCSLAWLSLHLGTTADVNHHKPHAR
                     CASWNPCDDPNPTITVCCGDYLTEPHYSGDANKWSTTGESLMRDMLTYRSRLARGCSC
                     FSGQACD"
     gene            6738..8027
                     /locus_tag="VEO00_11585"
     CDS             6738..8027
                     /locus_tag="VEO00_11585"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018836618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HYU58675.1"
                     /translation="MGVIVQKYGGTSVADTERIVSVASRIVGARRAGDDVVVVVSAMA
                     DMTDDLLAMAQEIAPVPVSRELDLLLTAGERIAMSLLSIAINHGGVPAVSFTGSQAGI
                     ITDTAHGKAKIIDIRPGRVREALDRGNVVIVAGFQGVSTAYDVTTLGRGGSDTTAVAL
                     AAALGADACEIYTDVPGVFTADPRIVPEARKLHAVSYEEMLELAACGARVLQLRAVEY
                     ARNHRVMVHVRSSFSGEPGTWVTEEDERMEQAIISGVAHDESEAKVTIADVPDRPGVA
                     AAIFRAVAEAGINVDMIVQNVSHDGRTDVSFTLPKDDLVHVRPVMSKAVEAVGARSVT
                     VDDGIAKVSLVGAGMKSHPGVAATMFEALADAGINIEMISTSTIRVSCVVRAGDVEQA
                     VRAIHDRFALSDEVILRESHPDGAFGVGARPAGEGPG"
     gene            8038..>8335
                     /locus_tag="VEO00_11590"
     CDS             8038..>8335
                     /locus_tag="VEO00_11590"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683199.1"
                     /note="catalyzes the formation of 4-aspartyl phosphate
                     from aspartate 4-semialdehyde; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HYU58676.1"
                     /translation="MRLAVVGASGAVGREMLRALAERRFPAGEIRAFATARSAGRRVP
                     FGDAELTVEEYGDGALAGIDLALFSAGGAAARTIVPGAAEAGTVCVDNSSAFRLE"
BASE COUNT         1137 a         2950 c         3067 g         1181 t
ORIGIN      
        1 tggtcatctc caatctgacg gtgccgggcg accgggggac ggacgcggtg cgcgacctgc
       61 acctcgaggt gcggtcgggc gagatcgtcg ggatcgccgg tgtctcgggc aacggccagc
      121 gggagctcgc cgaggcgatc gccggcctgc ggacgccgac gagcggctcc atccgcctga
      181 acggcgtgga gaccgccggg cgaggcccgg cggagatccg ggagcggggt ctgggctacg
      241 tcccggagga gcggacgcga gacggcatga tcgccgggtt cagcgtcagc gagaacctca
      301 tgctggtcga ccaccgcgac ccccggttct cacggtgggg attccttcgc ccgggcgcca
      361 tccagcgaca ttgcgcggag ctcgtctcgg ccttccgggt gaagacgccc gggctgtcca
      421 cgcccggatc gaacctgtcg gggggaaaca tccagaagct cgtactggct cgcgagctgt
      481 ccgggagccc gcgcgtcctg ctggtggccc agcccacgcg aggggtcgat gtgggcgcgg
      541 ccgagtacat ccagacccgg ctgctggagc agcgcgggca cgggaccgcc atcctgatca
      601 tctcggggga cctcgacgag gtactcgctc tctcggatcg catcctggtc atgtacgagg
      661 ggacgatcat cggggaggtc gacgcgcaga gtgccacccg ggaggccctg ggactgatga
      721 tggccggcgt gcgcccagac gcccaggagg aagcgcgggc ctgagacgga cgaccggagg
      781 tcgatccgga ctatcgggtc tcggccctgg cgaacaccga tatgccttcg cgagcggagt
      841 ggggacggag catgtccgcg ttcgcatact ccttgccctc gcgctcggcg atcaggcggc
      901 ccagttcgtt cgcctcgtcg tgggacagtc ccacgcggtc gcgtttctgc ttgagctgtc
      961 gatagcgatc ctccacccca tccctcctca tcggcgtccg tccccctcgc cgtcactggg
     1021 tgtcggcccg gtcgaacagg tccttcagct tcgtctcgat ggcgtccagg ctctctccgg
     1081 tcctctcccg gatggttccg accagccggt cccagttccc ctcgtcgcga tcgaggtcgt
     1141 cgtcggtgag cgatccccac gcctccttca cccggccctt gacctggtcc cacctgccct
     1201 ccatgcgcat cctgaagtcg gcgtccatga ccgctccttt ggtctcgtcg tccgtcggcg
     1261 tcattgccga catacccacg ccgccctcgt tccacgcgcg ggagggtccc gtatcgtgag
     1321 gtgcgtgctc gacgaggaac tgatggacgc ggccctcgag gccgcccgcg tctgccaggc
     1381 ctggggcgat gtcccgatcg gggcggtggt cgcgcgcgac ggcgccgtgc tggcctccgc
     1441 cgggaaccag cgcgagcggg cgcaggaccc gacggctcac gccgagattc tggcgctgcg
     1501 ggatgcagcc gccgcggcgg ggtcctggcg cctcgatgga tgcgacgtct tcgtcacgct
     1561 cgagccgtgc gccatgtgcg cgggcgcctt ggtcctcgca cgtgtcgacc gcctggtcta
     1621 cgcgacgccc gacccgaagg cgggcttcgc cgggagcctc ggcgacctga cccgcgatgc
     1681 ccggctgaac caccgcctgg aggtcgtttc cggcgtccgc gaggccgagg cgtccgagct
     1741 gctgcgagcc ttcttccgcg gccgccgcgg ggccacggag ggcgtctgag gccgttcgct
     1801 acaatggccg gcggaagcgt ggccgagcgg acgaaggcga tcgcctcgaa agcgattagg
     1861 ggttaaccgc ccctcgcagg ttcgaatcct gccgcttccg ccgcggctgg agccctttgc
     1921 ggtcccccta caatcgctgc ggtggcttgc gagagcggac gaatcggcac gcctggagag
     1981 cgtgtagggg ggtgaccccc tccagggttc gaatccctga gccaccgccg gagcgcggcg
     2041 gtcgggctgt ccgacgggcc gcgtatactc ggtcgcggtg accgtgctag gcggggagct
     2101 agcggtgccc tgcacccgca atccgctcga gcggggccga tgttcgcctt cggcccggac
     2161 ggtccggcag gcccggtgta cggggcgctg atggtcgggt cccgcgcgac gtcaacccgt
     2221 gaaccccgtg aggtccggaa ggaagcagcg gtaagcgggc gctggcgggt gccgcgggga
     2281 tgcctggccg gagccaccgg caccggatac cgccgagccg ttcgcggtca acggcggatg
     2341 cacggtcacc accttcttcc ccccgatgag gcgagggtcg tgtggacgtg accgagtcga
     2401 agacccgaac cgggaacggc gcccggaccc ccgccgccct gcgggccgag ccccatcgct
     2461 cgctctaccg caagtaccgc ccgcaggggc cgtccgaggt catcgggcag gagcacgtca
     2521 tccgcgccct ggtcggcgcg atccgcgagg gacgactggc ccacgcattc ctgttctgcg
     2581 gccctcgtgg gaccggcaag acctccaccg cgcgcatcct ggccaagatg gtgaactgcg
     2641 agcgcggccc gacacccgag ccctgcggcg tgtgcgagca gtgcgtcgcg atccgcgacg
     2701 gtcagcacct cgacgtcgtc gagatcgacg ccgcctccca cggcggcgtg gaggacgcgc
     2761 gggagctgcg cgagcgtgcc cccaccgctc cggcgcaggg ccgggagaag gtctacatca
     2821 tcgacgaggc gcagcggctg tcgcgcgagg cgttcgacgc gctgctgaag ctcttcgagg
     2881 agccaccgtc cggcgtgcgc ttcgtcctcg ccacgaccga gccgcacaag atgccggcca
     2941 cgatcatcgg ccgctgccag cggttcgact tccgccggat cgcctgggac gccctggcgg
     3001 ggcacctcgc ggcgatcgcg gagcgcgagg gggtctcact cgccccggag gccgctcaga
     3061 ccatcgcccg gcacgcggag ggctcggcgc gcgacgcgct gtcgctcctc gaccaggcca
     3121 gcgtgctcgg cgacggcgac gtggacgagg agaccgtgct cagcctgatc ggcgcggccg
     3181 acctcgaggt ccgcttcgcc ctggccgacg cggtggcggc gcaggatccg cgcacggtgt
     3241 tcgagatcgt ccaccggctt gtgcaggagg ggttcgacct gcgcagcgtc accgcgcagg
     3301 tgatcgccca cttccgcgac ctgttcctgg ccctcgtggc cgtggatcag gccgacctgc
     3361 tcgacgttcc ggccgagcac caggctcgcc tggcggccca ggcggcgaag ttcacgcccg
     3421 ccgagctgaa ccggatcctc tcggtgctgc tcgaggcgaa cacgaacttg cgctggacga
     3481 cgtcgccgcg gctgacgctc gagctggcgc tggtcaaggc ggccgtcccg gaggcggacg
     3541 cgcacgccga ggggctgctg tcgcgcatcg agcgcctgga gcggctctcg ggcctgaagg
     3601 acgcgccccc ggcctcgcca gaatcgccgg ccccagcgcc ggccccaccc ccgtccccgg
     3661 cgcctgccga ggagcgggtc accgaggagc cggccaccga gccacatgcg ccgtcgacgg
     3721 gagtggtcga taccgacctg gttcggcggt cgtggcccga ggtcctcaag gcgctcgaca
     3781 cggcgtcgaa ccgcgtgctg cacacggtgg cgcagctggt ggtcatgcag accctcgatg
     3841 gcgacacgct cgagctcgtc gttccggccg gcaagaagtt cgccgcggag aagatcatgc
     3901 agaagcagca ggagctccgc gccgtgctgc gagacatcct cgggatcacg ccggccctgc
     3961 gctgcgtggt ccgcgacggg tcgccggcca cggacgaccc gatcctcgaa gacgaggctc
     4021 cgccgacccc cgagctggcg atcgagcgcg tgatggaggg actgggcgcc gagatcgtcg
     4081 aggactaggc cccgggcgtg tagggtcggc acgtgttcga gggaccgatc cagaacctca
     4141 tcgacgagct gaaccggctc cccgggatcg gcccgaagtc cgcgcagcgc ctggccttct
     4201 acctggtcaa ggcgcccgcc gctgatgcca agcgcctggc cgaggcgatc gtccaggcga
     4261 aggagcgggt cagcttctgt cgtgagtgct tcaacgtggc cgagggcgcg ctctgccgct
     4321 tctgcgcgga cccctcgcgc gacgcgtcgg tgatctgcgt ggtggaggag ccgaaggacg
     4381 tggcggccat cgagaaggcg gggatcgtcc gcggccgtta ccacgtgctc ggcggcgcca
     4441 tctcgccgat ggaggggatc ggacccgacg acctgcgcat ccaggagctg ctcgaccggg
     4501 tcgggcgcga ccacacgcag gaggtcatcg tggcgaccaa cccgaacctg gagggcaacg
     4561 ccacggcgat gtacgtcgcc ggcctgctga agccactcgg cgtcagggtg acccgcctgg
     4621 cgagcgggct cccggtgggc ggggacctcg agtacgcgga cgaggtcacc ctcggccagg
     4681 cgctggaggg ccgccgcgag ctctaggcgt ccccggagta tcccggacgg tcagccttgc
     4741 agcgaatcgg ccacacgctg gatctcccgg atggcctcct cctcgaccgc ggcgatcgcg
     4801 gcgtccccgt cggtcccggc ggggggttcg atgaatccgg tgccgaggcg cagggtgagg
     4861 tggccgcacg cggcgtccgc ctcgcgccag ccgagcgcga gctccccgac ccccggatcg
     4921 cccgcccaga ccaggacgct cgccgatccc cggaccgtgg gcgtggccgc cgccgcgttc
     4981 gggtcgagga gcgcgacgtc actcgcctcg atcgtcacgt atccggcgcc ggagtcgccc
     5041 ggcgtcccgg cgaaccacgt ggccgaggcg tcgcgagtct catccggcgg tcccgggtcg
     5101 ccctcgcccg accagggcag gtactgcggc cggagggccc agggcgaaca cccggacccc
     5161 tcgccggtgt cgtccgctgc cggggtcgcc gtctccccgc aggcggcgag cagcaagagg
     5221 gccagcgcca cgccggccgg cacggtcttc gccacgcgcc ggcgcgaggg cgaaccgtcc
     5281 cccggcccgg gggagagccg agcagtcatc ccgatacctc ccgatccgtg accgcgggac
     5341 ggcggtcacc gcagggcgcg agcgtcacct cggagccggc gacggtccag ggcccgatgg
     5401 agagcaggcg gacgcgcgtc acctcccgcc ggcggcggtc gagcgcgaac ccgacgtgtg
     5461 tggccgacgt gcccggtcgc aggcggaaca cgacgaaggc gtcccgggga ccgtccgccc
     5521 acacgtcctc ggggcacggg ccgtccaggt ggcagcgcag gcggcggtag gcgggcgagc
     5581 gcttcgccgc cagcaggagg tcgctcgggt agcgcccgtc cgcgtcggcc gggagtcgcc
     5641 cggcgcacgg aagcggtcga gggcgcatcg ccgctcagtc gcaggcctgc ccgctgaagc
     5701 acgagcatcc tcgcgccagc cggctgcgat aggtgagcat gtcccgcatg aggctctccc
     5761 cggtggtgct ccacttgttc gcgtccccgg agtagtgcgg ctcggtcagg tagtcgccgc
     5821 agcagacggt gatggtcggg ttcgggtcgt cgcacgggtt ccaggacgcg cagcgcgcgt
     5881 gcggcttgtg gtggttgacg tcggccgtgg tcccgagatg cagagacagc caggccaggg
     5941 agcagagctg ggcgttgctc ggggcggaga cgttgccacc gttgcagccg ccgaaacagc
     6001 cgtagagcat gatcccgacg gcccggcagt tgcagccgcg ggcgtgcgcc ccggtcgagt
     6061 cgtcccagcg accgttgacg cagatgtcgc cgctgcggtc gatgcagaag tcgtagtagt
     6121 agcagtcggc gcacgcgtcg gtctcgaggt gggcgttcgg cgcgccggtg tgatggacca
     6181 tgatccagtc cgccctgggg gcgccctggt agtggccggg cagcccgcgt ccgccaccga
     6241 acggccaggc cacgccgctc cagtcgccgt tcctcggcgc gtcgatccgc cgcaagcgga
     6301 ccgggccggc ctcggcgcca ggatcgaggc ccgtcgcccc gggagtgcag taccgtggcg
     6361 gccggacctc ctcgcctccc ccggccgctc ccggttcgcg gggctgcgcc gcccacagcc
     6421 gtggcagcgg aagcgccacg ctcgaggtgg ccaggatcgc catctgcatg aagtgcctcc
     6481 gcgtcaccgt tggcgtcgtt tcgcggaacc gagcctgcag gcccatggca cctcccctct
     6541 ccggccctct gggggaggtg tccagcgaac cgtatatgcg ccacgcgccg gcgtcgtccc
     6601 ccatccgggg gttcccgggc cccgaccggg ggattcccgg ccgagccacc gccggaaggc
     6661 tgacgggctc cgtgagatgg cctgggtcca ccgggcgaag atccccgcgg caaggctgct
     6721 gctaggctcg ctcggcgatg ggcgtcatcg tgcagaagta cggcggtacc tcggtggccg
     6781 acaccgagcg gatcgtctcc gtggcctcgc ggatcgtcgg cgcccgccgg gccggcgacg
     6841 acgtcgtcgt ggtcgtctcg gcgatggcag acatgaccga cgacttgctg gccatggcgc
     6901 aggagatcgc ccccgtgccg gtctcgaggg agctcgacct gctgctgacg gcgggggagc
     6961 ggatcgcgat gtcgctcctc tcgatcgcca tcaaccacgg gggcgtgccg gccgtctcgt
     7021 tcacgggctc gcaggccggg atcatcaccg acaccgcgca cgggaaggcc aagatcatcg
     7081 acatccgccc ggggcgggtc cgcgaggccc tcgatcgtgg gaacgtggtc atcgtggccg
     7141 ggttccaggg tgtctccacc gcctacgacg tgacaacgct cgggcgcggc ggctcggaca
     7201 ccaccgccgt ggcgctcgcc gcggcgctcg gggccgacgc gtgcgagatc tacactgacg
     7261 tgcccggggt gttcaccgcc gacccccgga tcgtgccgga ggcgcgcaag ctccacgccg
     7321 tctcgtacga ggagatgctc gagctggcag cgtgcggcgc gcgagtgttg cagctgcggg
     7381 cggtcgagta cgcgcggaac caccgcgtga tggtccatgt gcgctcgtcg ttctccggcg
     7441 agcccggaac gtgggtcacc gaggaggatg agcggatgga gcaggcgatc atctcgggcg
     7501 tggcgcacga cgagtccgag gcgaaggtca cgatcgcaga cgtgcccgac cgtccgggcg
     7561 tcgccgcggc gatcttccgg gcggtggccg aggccgggat caacgtggac atgatcgtgc
     7621 agaacgtctc gcacgacggc cggaccgacg tctccttcac cttgccgaag gacgacctgg
     7681 tgcacgttcg gccggtcatg tcgaaggccg tcgaagcggt cggcgcgcgc agcgtcaccg
     7741 tggacgacgg catcgcgaag gtgtcgctgg tcggcgcggg gatgaagagc caccccggcg
     7801 tggccgcgac gatgttcgag gcactcgccg acgccgggat caacatcgag atgatctcga
     7861 cctcgacgat ccgcgtgtcc tgcgtcgtcc gcgccggcga cgtcgagcag gcggtgcgcg
     7921 cgatccacga tcggttcgcg ctgtccgacg aggtgatcct gcgcgaatcc cacccggacg
     7981 gcgcgtttgg ggtgggagcg cggcccgcgg gagaggggcc cggctaggat ggccggcatg
     8041 cgcctcgcgg tcgtcggtgc ctccggagcc gtcgggcggg agatgctccg ggccctcgcc
     8101 gaacgccgct tcccggccgg cgagatacgc gccttcgcca ccgcccgatc ggccggccgg
     8161 cgcgttccgt tcggggatgc cgagctgacc gtggaggagt atggggacgg ggcgctggcc
     8221 gggatcgacc tggccctgtt ctcggccggc ggcgcggcgg cccggacgat cgtcccgggc
     8281 gccgcggagg ccgggaccgt gtgcgtggac aactcctcgg cgttccggct ggagc
//
LOCUS       DASPWO010000505         5319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_72193, whole genome shotgun sequence.
ACCESSION   DASPWO010000505 DASPWO010000000
VERSION     DASPWO010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72193"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VEO00_11595"
     CDS             <1..384
                     /locus_tag="VEO00_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58677.1"
                     /translation="YEDLVADPERELEAICAFAALDFTPELLTYYERADEVLTDLEKG
                     RHPKVKEAPAVVRDWRRDMPRKAVAMVEAVAGDALEEFGYLRATPTAPAGVRMRAKAS
                     LGVRRFRRRLKRRGRAAKARLSGAD"
     gene            399..1385
                     /locus_tag="VEO00_11600"
     CDS             399..1385
                     /locus_tag="VEO00_11600"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYU58678.1"
                     /translation="MSALERASGPPIFVVGCPRSGTTMLRLMLDSHPDLAIPPESHFV
                     TQFWSAHHRYEKNDRFEADRLARDVMGTFRFKEWRLPPEAVTERLRRLERPSFGSVME
                     AFFLAYADREGKTRWGDKTPGYSIEMAALAQIFRDARFVHLIRDGRDVAMSLDDLGRV
                     KLRPAMAMWAQRVMQGRSDGRRLGPDRYLEVFYEALVDDPERELKAICDHADLEFRPG
                     DMLRYHERAERAMASKAWGSHQGLARPPTKGMRDWRRSLDDRTQAVLEGIGGDALTEF
                     GYERRFSDPPAVARREADRVVRGYRRAMRAWRLRRRAIMILKPRAVPPHRRF"
     gene            1388..2398
                     /locus_tag="VEO00_11605"
     CDS             1388..2398
                     /locus_tag="VEO00_11605"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYU58679.1"
                     /translation="MPDPAGRPAPAPGGPLFVVGCGRSGTTLLRLMLDANPALAIPGE
                     SHFIPPLWRARRRYERDGAFDEALLAEDLLRVPHVRAWGVPESVMRAYLGNAAGGSFA
                     DIVSAAFDAYAAHHGKVRWGDKTPIYVLDMPLLADLFPDARFVHLIRDGRDVTLSYLS
                     VSWGPRSVWEAARKWRRDVTAGRLHGQELGKARYLELRYEDLVADPRTWLETVCAFAD
                     LAFDERMLEHNKDWRDRVQAPPGGAERWHRSVAKPVTEGLRDWRTQMAERDVRAFETI
                     AGRLLTDLGYERRFPRIGLARRAGAGLRVGWMESVAAGSRAKKAFNRARGRAPAKIDR
                     GR"
     gene            complement(2400..2771)
                     /locus_tag="VEO00_11610"
     CDS             complement(2400..2771)
                     /locus_tag="VEO00_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYU58680.1"
                     /translation="MTDRATVERWLEGYQQAWGSNDPADIEALFAEGARYWTAPHREP
                     WTGRAEIAREWIARKDDPGTWSFRSEILAIAGDLAFVRGWTDYPGQDPPLYSNLWVIR
                     FDDDGRATEFTEWWMPAGPTD"
     gene            complement(2768..3748)
                     /locus_tag="VEO00_11615"
     CDS             complement(2768..3748)
                     /locus_tag="VEO00_11615"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HYU58681.1"
                     /translation="MEITTKKRMMLFTGSADPPLAKEIADHLGMRLSDVKLSRFASGE
                     IYVRFDDSVRGSDAFVIQSHADPVNESIMEQAIMIDALKRASAKRITAVVPYYGYSRQ
                     DKKTLAREPITAKLVADILTVAGADRVMSVDLHSGQIQGFFDFPFDHLTALPILAGYL
                     REELGLEDGNLVVVAPDAGRVKTAERLREQLDSELAYIYKRRSRLEAHKIETMVVEGD
                     VDGRACVLVDDMIDTAGTITAGAQVLREHGATDIYAAATHPVFSGEAVKRLADAPIRG
                     VVVTNTLPIPEDRRLDKITVLSVAPIMAAAIKAVFEEESVSELFHGENMP"
     gene            complement(3773..5215)
                     /gene="glmU"
                     /locus_tag="VEO00_11620"
     CDS             complement(3773..5215)
                     /gene="glmU"
                     /locus_tag="VEO00_11620"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HYU58682.1"
                     /translation="MATPRRTRTLAAVVMAAGKGKRLKSDTPKVLHPVAGRPSLWHVL
                     NAVRAVRPDRTVVVVGVGKERVEVAVRAWWPKDRFTFVDQGEQLGTAHAVLAAERAVG
                     RAHDVLVAAGDEPLVTAEHLRGLLRVHRRRDMAAVIQTTVPDDARGFGRIIRDGDDFV
                     RIAEGSDATPAELAVTEVGTSVYAFRRDALFAALPLVDRENRQREHYLPDVLGILREK
                     GERIAVQTVDFGGAIGANSRAEIARANAVFRRRLNEAHMAAGVSIVDPETAFVDVGVR
                     IGRDATILPFTFLEGDTRVGPGATVGPSTRLVDTTVGAGATVQFTVANGARIGPRATV
                     GPFTYLRPEAVLEEGSKAGTFVEIKASRVGKGSKVPHLSYVGDARIGRGANIGAGTIT
                     VNYDGYEKHPTYVGDGARVGSDTMLVAPVRVGRDAFTGAGSAITRDVPPGALAVERSK
                     QVTVRGYAKRRAETARRAASGGGSGKRARR"
BASE COUNT          719 a         1906 c         1925 g          769 t
ORIGIN      
        1 tacgaggacc tggtcgccga tccggagcgc gagctggagg cgatctgcgc gttcgccgcc
       61 ctcgacttca ccccggagct cctgacctac tacgagcgcg ccgacgaggt gctgaccgac
      121 ctggagaagg gtcgacaccc gaaggtgaag gaggcgccgg ccgtggtccg agactggcgc
      181 cgcgacatgc cgcggaaggc cgtggcgatg gtcgaggccg tggcggggga tgccctggag
      241 gagttcggct acttgcgcgc gacccccacc gcccccgcgg gcgtgcgcat gcgggcgaag
      301 gcctccctcg gggtccggcg cttccggcgc cggctgaagc ggcgggggcg agcggcgaag
      361 gcccggctct cgggcgccga ctagactcga ccgccggtat gtcggcactc gaacgggcca
      421 gcggaccgcc catcttcgtc gtcggctgcc cacgctcggg caccacgatg ctgcggctga
      481 tgctcgactc gcaccccgac ctggccatcc cgccggagtc gcacttcgtc acgcagttct
      541 ggtcggcaca ccaccgctac gagaagaacg accggttcga ggccgatcgc ctggcccgcg
      601 acgtgatggg gacgttccgg ttcaaggagt ggcgcctgcc gccggaggcg gtcaccgagc
      661 gcctgcgccg gctggagcgc ccgtctttcg gctcggtgat ggaggcgttc ttcctggcct
      721 acgccgaccg cgaggggaag acgcgctggg gggacaagac gcccggctac tcgatcgaga
      781 tggcggcgct cgcccagatc ttccgcgacg cacggttcgt gcacctgatc cgcgacgggc
      841 gcgacgtggc gatgtcgctc gacgacctcg gtcgcgtgaa gctgcgaccg gcgatggcca
      901 tgtgggcgca gcgggtgatg cagggccggt cggacgggcg ccgcctcggc cccgaccgct
      961 acctggaggt cttctacgag gcgctggtcg acgaccccga gcgcgagctg aaggcgatct
     1021 gcgaccatgc cgacctggag ttccggcccg gggacatgct gcgctaccac gagcgcgccg
     1081 agcgggccat ggccagcaag gcctggggca gtcaccaggg cctggccagg ccgccgacga
     1141 aggggatgcg cgactggcgg aggtcgctcg acgaccggac gcaggcggtg ctcgagggga
     1201 tcggcggcga cgcgctcacc gagttcggct acgagcggcg gttctccgat ccgccggccg
     1261 tggcccgccg cgaggccgat cgcgtcgtcc gcggctaccg ccgggcgatg agggcctggc
     1321 gcctccgccg ccgcgcgatc atgatcctga agccccgcgc cgtgcccccg caccgccgct
     1381 tctagccgtg ccggacccgg ccggacggcc agcgcccgcc cccgggggcc cgttgttcgt
     1441 ggtcgggtgc gggcggtcgg ggaccacgct cctgcggctg atgctcgacg ccaatccggc
     1501 gctggcgatc ccgggggagt cccatttcat cccaccgctg tggcgggcgc gcaggcgcta
     1561 cgagcgcgac ggggccttcg acgaggcgct gctggccgaa gacctcctgc gcgttccgca
     1621 cgtgcgggcc tggggcgtgc cggagagcgt gatgcgcgcg tacctcggga acgccgccgg
     1681 cggctcgttc gccgacatcg tgtccgcggc gttcgacgcg tacgccgcgc accacggcaa
     1741 ggtccgctgg ggcgacaaga ccccgatcta cgtgctcgac atgccgctgc tggccgacct
     1801 gttccccgat gcgcgattcg tgcacctgat ccgcgacggg cgcgacgtca cgctctccta
     1861 cctgtcggtg tcgtgggggc cgcggagcgt gtgggaagcc gcgcggaagt ggcggcgcga
     1921 cgtcaccgcc ggacggctgc acggccagga gctcgggaag gcgcgctacc tggagttgcg
     1981 ctacgaggac ctggtcgccg acccgcgcac ctggctggaa accgtgtgcg cgttcgccga
     2041 tctggccttc gacgagcgca tgctcgaaca caacaaggac tggcgcgacc gcgtccaggc
     2101 gccgccgggc ggcgcggagc gctggcaccg ttcggtggcc aagccggtca ccgagggcct
     2161 gcgcgactgg cgcacgcaga tggccgagcg cgacgtccgg gcgttcgaga cgatcgccgg
     2221 ccgcctgctc accgaccttg gctacgagcg ccgtttcccg cggatcggcc tcgcccgccg
     2281 cgccggggcc gggttgcgcg tcggctggat ggagtcggtc gccgccggga gccgcgccaa
     2341 gaaggccttc aaccgcgccc gaggccgggc gccggcgaag atcgaccggg ggcgctgagt
     2401 cagtcggtgg gtccggccgg catccaccac tcggtgaact cggtggcccg gccgtcgtcg
     2461 tcgaaccgga tcacccagag gttcgagtag agcggcggat cctggcccgg gtagtcggtc
     2521 cagccgcgga cgaaggccag gtcgccggcg atcgccagga tctcggagcg gaacgaccac
     2581 gtgccgggat cgtccttgcg ggcgatccac tcccgggcga tctcggcgcg cccggtccac
     2641 ggctcgcggt ggggcgcggt ccagtacctc gcgccctcgg cgaacagcgc ctcgatgtcg
     2701 gccgggtcgt tcgaccccca ggcctgctgg tagccctcca gccagcgctc gaccgtggcg
     2761 cggtcggtca cggcatgttc tcgccgtgga acagctcgga gaccgactcc tcctcgaaga
     2821 cggccttgat cgcggcggcc atgatcgggg ccaccgagag gaccgtgatc ttgtccagcc
     2881 gccggtcctc ggggatcggc agcgtgttcg tgaccacgac ccccctgatc ggggcgtccg
     2941 ccagccgctt gacggcctcg cccgagaaca ccgggtgcgt cgcggcggcg tagatgtcgg
     3001 tggcgccgtg ctcacgcagc acctgcgcgc cggcggtgat cgtcccggcg gtgtcgatca
     3061 tgtcatccac cagcacgcag gcccgtccgt cgacgtcgcc ctcgaccacc atcgtctcga
     3121 tcttgtgcgc ctccaaccgg gagcgtcgct tgtagatgta cgcgagctcc gagtcgagct
     3181 gctcgcggag ccgctcggcg gtcttgacac gcccggcgtc gggcgccacc accaccaggt
     3241 tgccgtcctc cagcccgagt tcctcgcgca ggtagccggc caggatcggc agcgccgtca
     3301 ggtggtcgaa cgggaagtcg aagaacccct ggatctgtcc ggagtgcagg tcgaccgaca
     3361 tgacgcggtc ggcgccggcg accgtcagga tgtcggccac cagcttcgcc gtgatcggct
     3421 cgcgggcgag cgtcttcttg tcctggcgcg agtacccgta gtagggaacg acggccgtga
     3481 tccgcttcgc cgacgcccgc ttcagcgcgt cgatcatgat cgcctgctcc atgatcgact
     3541 cgttgaccgg gtcggcatgg ctctggatga cgaatgcatc cgagccgcgg acgctgtcgt
     3601 cgaagcgcac gtagatctcg ccggaggcga agcggctgag cttgacgtcg gacagccgca
     3661 tccccaggtg atcggcgatc tccttcgcca ggggagggtc cgcgctcccc gtgaagagca
     3721 tcatccgctt cttggtcgtg atctccacgg atcccctccc cgagaccgct cgctatcgcc
     3781 ttgcgcgctt ccccgaaccg cccccggacg cggcgcgacg ggccgtctcc gcccgccgct
     3841 tcgcgtaccc ccgaacggtc acctgcttgc tccgctcgac tgccagggcg cccggcggga
     3901 catcgcgggt gatcgccgat cccgcgcccg tgaaggcatc cctgccgacc cggaccggcg
     3961 ccaccagcat ggtatcggac ccgacccgcg ccccgtcgcc gacgtaggtc gggtgcttct
     4021 cgtagccgtc gtagttcacg gtgatggtgc cggcgccgat gttcgcgcct cgcccgatcc
     4081 gcgcgtcgcc gacgtaggag aggtggggaa ccttgcttcc cttcccgacc cgcgaggcct
     4141 tgatctcgac gaacgttccg gccttcgagc cctcctcgag cacggcctcc ggccggaggt
     4201 aggtgaacgg cccgacggtg gctcgtggcc cgatccgtgc gccgttcgcc acggtgaact
     4261 ggaccgtggc gcccgcgccg accgtggtgt cgacgagccg cgtcgagggg ccgaccgtcg
     4321 cccccggacc gacgcgggta tcgccctcca ggaacgtgaa cggcaggatg gtcgcgtcgc
     4381 gcccgatgcg cacgccgacg tcgacgaacg ccgtctccgg gtcgacgatc gacacgccgg
     4441 cggccatgtg cgcctcgttc agtcgccggc ggaacacggc gttggcgcgg gcgatctcgg
     4501 cccgcgagtt cgccccgatc gccccaccga agtccacggt ctggacggcg atccgctcgc
     4561 ccttctcgcg caggatgccg agcacgtcgg ggaggtagtg ctcccgctga cggttctcgc
     4621 ggtcgacgag cgggagcgcg gcgaagagcg cgtcgcggcg gaaggcgtac acggaggtgc
     4681 ccacctcggt caccgcgagc tcggccggag tcgcgtcgga gccctcggcg atgcgaacga
     4741 agtcgtcgcc gtcgcggatg atcctcccga acccgcgagc gtcgtcgggg acggtcgtct
     4801 ggatcacggc ggccatgtcg cggcggcggt ggacgcgcag caggccgcgg agatgctccg
     4861 ccgtgaccaa cggttcgtcg ccggcggcga ccagcacgtc gtgggcgcgc ccgacggccc
     4921 gctcggcggc gaggaccgcg tgcgcggtgc cgagctgctc gccctggtcg acgaacgtga
     4981 accggtcctt cggccaccaa gcgcgcacgg ccacctcgac gcgctccttg ccgacaccga
     5041 ccacgacaac ggtccggtcc ggccggacgg cccgcacggc gttcaggacg tgccacaggc
     5101 tcgggcggcc ggcgaccggg tgcaggacct tcggcgtgtc cgacttcagc cgcttgccct
     5161 tcccggccgc catgacgacg gcggccaggg tgcgggtccg tctcggggtg gccaaggagc
     5221 gtcctctccg gggaaggtgg ggttcgggtg tgttctacag gcgcccggca gaatctggca
     5281 aggcatcgat cataggcggg agcggttcga cctgaaggg
//
LOCUS       DASPWO010000506         6143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_494666, whole genome shotgun sequence.
ACCESSION   DASPWO010000506 DASPWO010000000
VERSION     DASPWO010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494666"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VEO00_11625"
     CDS             <1..585
                     /locus_tag="VEO00_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HYU58683.1"
                     /translation="GTPVARSYTITNNLAAFTGRAVGTTLGSALRGRFTIANLEQQER
                     TVNVTAGSTSLRATIGNPSDPGADLDLFVFNCTSGTCVLAGQSADGDSEESVTINNPA
                     AGEWLVLVDGFSVPAGTTQYDYVDVFVNPAFGSISVTDANALRPSGSSWTVPASVTAN
                     SAPAAGRVLLGNVQVRTDVNVLVGQGDVVVENVT"
     gene            complement(672..863)
                     /locus_tag="VEO00_11630"
     CDS             complement(672..863)
                     /locus_tag="VEO00_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58684.1"
                     /translation="MRLRFMLLFMAVASAGLIAGAAVTKMAWPLFFLFLALGAAIPLS
                     RTDEPPDGETPAGDPPARD"
     gene            966..1340
                     /locus_tag="VEO00_11635"
     CDS             966..1340
                     /locus_tag="VEO00_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYU58685.1"
                     /translation="MARYLLLIYDDENQWASMSPEDTGRMLQEYSTYGNELTSAGAMV
                     AGEGLQPTATATTVTLKGSDVVTTDGPYAETKEQLGGFYLVEARDLDDAIQWAAKIPS
                     AKRGGLIEVRPVQEFPDALEQH"
     gene            1337..2611
                     /locus_tag="VEO00_11640"
     CDS             1337..2611
                     /locus_tag="VEO00_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYU58686.1"
                     /translation="MSEAPAREVVDRLFRHESGRAVATLIRVTGDFDLAEEAVQEAFL
                     VALERWPRDGLPDNPGAWITVTARNKALDRLRREKRLAQKREALAALERLRVPAAGVE
                     ATAVPDDRLRLMFTCCHPALAPEARVALTLRTLGGLTTAEIARAFLVPEPTLAQRLVR
                     ARRKIRDAYIPYRVPPEHLLPERLPAVLAVLYLIFNEGYAATAGAALIRRELCAEAIR
                     LSRVLAALMPDEPEALGLLALELLQDSRREARTGPSGELVLLEEQDRGRWDRAEIDDG
                     QALLERAARLGPAGPYTLQAAIAAEHARAARAEDTDWVRIAALYGRLAARTRSPVVEL
                     NRAVAIAMARGPAVGLSLINRLGATGALDGYHLYHSARADLLRRLGRRAEAADAYRRA
                     LDLARNPVERAFLERRLAEAAPGPGSPVPDSP"
     gene            complement(3045..4268)
                     /locus_tag="VEO00_11645"
     CDS             complement(3045..4268)
                     /locus_tag="VEO00_11645"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYU58687.1"
                     /translation="MLTGRSRRAALLVLAVAAAACSGGVGPLTTPPPSTGSPPTSAGS
                     PMSGAFDPSRVSLALENLAGGLDGPLAVTGAGDGSGRLFVAEQTGRIRVIRDGELLPD
                     PFLDLSERVVVTSEQGLLGLAFHPDYAENGRFFVYYSAPGSEQRVAEFSVSADDPDRA
                     DPGSERILLPMADQAENHNGGCILFGPDGYLWIATGDGGEQGDPEGDAQDRGSLLGKL
                     LRMDVDGGDPYRIPPDNPFVDTAGARPEVWAYGLRNPWRFSIDRETGDVWIGDVGGSA
                     YEEVNRAPGGRAGLNFGWVVTEGPECYGGGTDCDRSGLVPPVWSYPTGCCVTGGFVYR
                     GSDFPVLAGGYLVGDYATGQIWSLDPAGPDTQEGVELLDSDLSISSFGEDDDGELYVT
                     DIGGGGLYRITATRA"
     gene            complement(4277..5346)
                     /locus_tag="VEO00_11650"
                     /pseudo
     CDS             complement(4277..5346)
                     /locus_tag="VEO00_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973192.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            5484..>6143
                     /gene="rmuC"
                     /locus_tag="VEO00_11655"
     CDS             5484..>6143
                     /gene="rmuC"
                     /locus_tag="VEO00_11655"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HYU58688.1"
                     /translation="MEINGVLIGLLALAVAGLAAALAVALRRASSPPPATSDVALQEA
                     VRSQALRLERVAATVEQGALGQEALRRGMDDTRALLADLSARDEERRRRDDEAARAWS
                     RLESVLLGGSSRGRMGENVVAEALSQLSPDVLERDVALNGKTVEFALRLPDGRRLPVD
                     SKWTAMAEAQQLEEADGDERVRLSQVVEKEAALRAREVAKYLGPASAPFAVAAVPDPV
                     YA"
BASE COUNT          880 a         2198 c         2240 g          825 t
ORIGIN      
        1 ggcacgccgg tcgcgcgcag ctacacgatc acgaacaacc tggccgcgtt caccggacgg
       61 gccgtgggga cgaccctcgg cagcgccctg cgcggccggt tcacgatcgc gaacctcgaa
      121 cagcaggagc ggacggtgaa cgtcacggcc ggctcgacgt cgctgcgggc gacgatcggg
      181 aacccgagcg atccaggagc cgacctcgac ctgttcgtct tcaactgcac cagcgggacc
      241 tgcgtcctgg cagggcagag cgcggacggg gactccgagg aatcggtgac gatcaacaac
      301 ccggctgccg gcgagtggct ggtgctcgtg gacggcttct cggtcccggc cgggacgacg
      361 cagtacgact acgtcgacgt gttcgtgaac ccggcgttcg ggagcatcag cgtgaccgac
      421 gcgaacgcgc tgcgcccgag cggttcctcc tggaccgttc ccgcgagcgt gaccgcgaac
      481 tcggccccgg ccgccggacg ggtcctgctg ggcaacgtgc aggtccggac cgacgtgaac
      541 gtcctggtcg gacagggcga cgtggtcgtc gagaacgtca cgtaggccgt cgcaggcacg
      601 cggggggccg cgccggatgt ccggtgtcgg ccctctcgca ttgcgcgccg gggtcccgag
      661 gaggggagtg gtcagtccct cgcgggggga tcgccggcgg gcgtctcccc gtcaggcggc
      721 tcgtcggttc gcgagagggg gatggccgcc ccgagggcca ggaacaggaa gaagagcggc
      781 caggccatct tcgtcaccgc cgccccggcg atcagcccgg ccgaggcgac ggccatgaac
      841 aggagcatga agcgcaggcg cacggagaga gcctagggaa tgatcgcccg gtctgtcgag
      901 atggtcgtgg cccgttcgtc gtatgaaggg gcggcgagcg gcgccgccgg cgagaggagg
      961 cgggaatggc caggtacctg ctgctgatct acgacgacga gaaccagtgg gcgagcatgt
     1021 ccccggagga caccgggcgg atgctgcagg agtactccac ctacggcaac gagctgacgt
     1081 ccgccggcgc catggtcgcc ggggaagggc tgcagccgac ggcgacggcg accacggtca
     1141 ccctgaaggg cagcgacgtg gtcacgaccg acgggccgta cgccgagacc aaggagcagc
     1201 tcggcgggtt ctacctggtg gaggcgaggg acctggacga cgcgatccag tgggcggcca
     1261 agattccgag cgcgaagcgc ggcggcctga tcgaggtgcg cccggtgcag gagttcccgg
     1321 acgcgctcga gcagcactga gcgaggcacc cgcgcgcgag gtcgtcgacc gcctgttccg
     1381 gcacgagtcg ggccgggcgg tcgcgaccct gatccgggtc accggcgact tcgacctggc
     1441 cgaggaggcc gtgcaggagg cgttcctggt cgccttggag cgctggccgc gcgacggcct
     1501 gccggacaat ccgggcgcct ggatcacggt caccgcccgg aacaaggcgc tcgaccggct
     1561 gcgccgcgag aagcggctgg cgcagaagcg cgaggcgctc gccgccctgg agcggctgcg
     1621 ggtccccgcg gcgggcgttg aggcgacggc cgtgccggac gaccgcctgc ggctgatgtt
     1681 cacgtgctgc cacccggcgc tcgcgcccga ggcccgcgtg gccctgacgc tgcgtacgct
     1741 cggcggcctg accaccgccg agatcgcccg ggccttcctg gtgcccgagc cgacgctcgc
     1801 gcagcgcctg gtccgggcga ggcgcaagat ccgcgatgcc tacatcccgt atcgcgtccc
     1861 gccggagcac ctgctgccgg agcggctccc ggccgtgctg gccgtcctgt acctgatctt
     1921 caacgagggg tacgccgcga cggccggggc cgccctgatc cgccgcgagc tgtgtgccga
     1981 ggcgatccgg ctgagccgtg tgctggccgc gctgatgccg gacgagcccg aggcgctcgg
     2041 cctgctggcg ctcgagctgc tgcaggactc acggcgcgag gcgcgcaccg ggccgagcgg
     2101 agagctcgtg ctgctcgagg agcaggatcg cggccgctgg gatcgggcgg agatcgacga
     2161 tggccaggcg ctcctggagc gcgcggcccg gctgggcccg gcgggtccct acacgttgca
     2221 ggcggccatc gccgccgagc acgcccgggc cgcgcgagcc gaggacacgg actgggtccg
     2281 catcgcggcg ctctatgggc gactggccgc gcggacccgt tcgccggtgg tcgagctgaa
     2341 ccgcgcggtg gcgatcgcca tggcgcgcgg cccggcggtc gggctgtcgc tgatcaaccg
     2401 cctgggcgcc accggggccc tcgacggcta tcacctctac cactcggccc gggccgacct
     2461 gctgcgccgc ctcggtcggc gcgcggaggc cgcggacgcc taccgccgcg ccctcgacct
     2521 ggcgcggaac cccgtggagc gcgcgttcct cgaacgccga ctggccgagg cggcgccggg
     2581 accgggctcg cccgtcccgg attccccttg acggccgaac tcgacttcat cgcgcccggc
     2641 tacggcagcg atccgtggaa cccgaacggc agccggtatt cagtcatctg gccgcgggtc
     2701 ttctactacg acgactgctc gcatacacag acgttcaacg atgggcgcac atacgagctc
     2761 ctgtaccggg gagccaccga caagacgaat gtcaacagga tcatcacgac tacgtccgac
     2821 gacaagcagc tctatggctg gacagatggc ggatcgccct caagtgggcg aacaacatcc
     2881 ggtcctgggt gaacgggtgg aactgctcga agtcgaccgg atccctgagg tggtctgctc
     2941 gggttacatc tgccctctcg tcgatccggg ctcgaactcc atcagttcct ctggcagcaa
     3001 gggggccagc ttctacgggg agggtacaga caagcagcct tacttcaggc ccgggtcgcc
     3061 gtgatccggt acaggccgcc gccgccgatg tcggtgacgt acagttcgcc gtcgtcgtcc
     3121 tcgccgaacg agctgatcga aaggtcgctg tccagcagct cgacgccttc ctgcgtgtcg
     3181 gggcccgcgg ggtcgaggga ccagatctgg ccggtggcgt agtccccgac caggtagccc
     3241 ccggcgagca cggggaagtc cgacccgcgg tagacgaacc cgccggtcac gcagcagccg
     3301 gtggggtaag accagaccgg cggtacgagg ccgctccggt cgcaatcggt cccgcccccg
     3361 tagcactccg ggccctccgt caccacccag ccgaagttca ggccggcgcg gcctccgggc
     3421 gcccgattca cctcctcgta ggcgctgccg ccgacgtcgc cgatccacac gtccccggtc
     3481 tcgcggtcga tcgagaaccg ccacgggttg cgcagcccat aggcccacac ctccggccga
     3541 gcgccggccg tgtccacgaa ggggttgtcg ggggggatcc ggtacggatc gcccccgtcg
     3601 acgtccatcc gcaggagctt cccgagcagc gacccacggt cctgcgcgtc gccctccggg
     3661 tcgccctgct cgccgccgtc gccggtcgcg atccacaggt agccgtccgg gccgaagagg
     3721 atgcaccctc cgttgtggtt ctcggcctgg tcggccatcg gcagcaggat ccgttccgag
     3781 cccgggtctg cacggtccgg gtcgtccgcc gagacgctga actcggcgac gcgttgctcc
     3841 gagcccggcg ccgagtagta gacgaagaac cggccgttct ccgcgtagtc cgggtggaag
     3901 gccagcccga gcaggccctg ctcggaggtg acgacgaccc gctcggagag gtcgaggaag
     3961 ggatcgggga gcagctcgcc gtcccggatg acccggatgc gccccgtctg ctccgcgacg
     4021 aacagccgac cgctcccgtc cccggcgccg gtcaccgcga gcggaccgtc cagcccgccg
     4081 gcgaggttct cgagggcgag cgacacgcgg gacgggtcga acgcgccgga catgggagag
     4141 cccgccgagg tcggcggcga gccggtcgac ggtggagggg tggtgagggg accgaccccg
     4201 ccgctgcacg ccgcggcggc gaccgccagc accaggagcg ccgcgcgcct cgatcggccg
     4261 gtgagcacac tcctccctac aacgtccgcc gcggctcggt tcggcgccgc cgcgcctgcg
     4321 gggccatcca cggcggggcc catccggtgt cggcctcctc gaacgtcacc cccggcggga
     4381 cgatcgcgtc gatcgcgtcg agggtctcgc cgtccaggcg gacgtccgcg ccggccagca
     4441 ggtcgtcgag ctgctccatg gtccgcggcc cgatgatcgc cgaggtcacg gccgggtgag
     4501 tcagggcgaa cgcgatcgcc atgtgcgtca gcgagatccc ggccttgtcc gccacccgct
     4561 ccagctcctc gaccgcctcg agcttgcgct ggttggccgg acggctcatg tcgaagcgct
     4621 gcacgaccgg cgacatctgc ccgtaggccg tggaacgggc ggcgcgggac tcggccggca
     4681 ccgcctgccc ccggcggtac ttgcccgaca ggtagccgcc ggcgagcggg ctccacacca
     4741 gcacccccat cccgtggcgg agcgtcaccg ggaacgtgtc gtcctccttg tgccggacga
     4801 agatcgagta cggcgctggt cgcaggcgaa ggactccagg ttgcgatccc gggcgaccca
     4861 gtgggcctcc tcgagctgcc atcccgtgta cgtcgaggag cccaggtagc gcaccttccc
     4921 ctgccggacc aggtccgtca gcgccgagag cgtctcctcg atcgcgatga tcgggtcggg
     4981 gcggtgcatc tgatacaggt cgatgcgatc ggtctgcagc cggcgcaggc tgtcctcgac
     5041 cgcccgcatg atccacagcc gcgagccgcc ccggtcgttc tggccctcgc ccatcgggtt
     5101 gaagaacttc gtcgccagca cgacctggtc gcgccgcccc ttcagggcac gcgcgacgat
     5161 ctcctcggac ccgcccgccg agtagacgtc ggccgtgtcg acgaaattga tccccgcgtc
     5221 gagcgccgcg tggatgatcc gcacgctctc gtcgggatcg cgattccccc actcaccgaa
     5281 catcatcgtc ccgaggcagt gggcgctgac ccgcaccccg gtccgcccga gcgttcggta
     5341 ctccatggcc ccctcctcgg cgccggctgc ttgcactccc cgccgaaccc atcttccccc
     5401 gatgggccgg aagagggtcc cgggtcccga gcgtgtcgta ccccccggct accctgttcg
     5461 aagcagcctc cgaaggggac gggatggaga tcaacggcgt gctcatcggg ctactggccc
     5521 tggccgtggc cgggctggcg gcggcgctgg cggtcgcgct gcggcgggcg tcctccccgc
     5581 cgccggccac gagcgacgtc gccctgcagg aggccgtccg gtcccaggcg ctccgcctgg
     5641 agcgcgtggc cgccaccgtg gagcaggggg cgctggggca ggaggcgctg cggcggggca
     5701 tggacgacac gcgggcactg ctggccgacc tctcggcgcg ggacgaggag cggcgccgtc
     5761 gggacgatga agcggcgcgc gcctggtccc ggctcgagtc ggttctgctc ggcggatcga
     5821 gccgcggccg gatgggggag aacgtggtgg ccgaggcgct ctcgcagctc tcgcccgacg
     5881 tcctggagcg cgacgtggcg ctgaacggta agacggtcga gttcgcgctg cgcctgccgg
     5941 acggccggcg cctcccggtg gactcgaagt ggacggcgat ggccgaggcg cagcagctcg
     6001 aggaggccga cggcgacgag cgcgttcgcc tgtcccaggt ggtcgagaag gaggccgcgc
     6061 tgcgcgcccg ggaggtcgcc aagtacctcg ggccggcgag cgcgccgttc gccgtggccg
     6121 cggtgcccga ccccgtgtac gcc
//
LOCUS       DASPWO010000507         4538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_921179, whole genome shotgun sequence.
ACCESSION   DASPWO010000507 DASPWO010000000
VERSION     DASPWO010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921179"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3030)
                     /locus_tag="VEO00_11660"
     CDS             complement(<1..3030)
                     /locus_tag="VEO00_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYU58689.1"
                     /translation="MSPLDRFSPLARGWFESAFAEPTPAQAEAWEAISTGRDALVVAP
                     TGSGKTLAAFLWALDRIATSPAPEQKKRLRVLYVSPLKALAVDVERNLRSPLSGMRAA
                     AERLGQAVPDVAVAVRTGDTPAEDRRRFATRPPDILITTPESLFLILTSQAREALANV
                     ETVIVDEIHAIAGNKRGAHLALSLERVDQLLERPAQRVGLSATVRPVDEVGRFLTGGR
                     PVEILQPLAEKTFDLKVVVPVEDMTAPGGEVPADGGGPGGDPEERASVWPAVEREIVD
                     LIRAHRSTIVFANSRRLAERLCARLNAIAREDAADPEGPIARAHHGSVSRDQRLQIED
                     DLKAGRLPAVVATSSLELGIDMGAVDLVIQVEAPASVAQGLQRIGRAGHQVGAVSRGT
                     VFPKYRGDLVECAVVVERMRAGAIEALSVPRNPLDVLAQQVVSMVAMDPWTVDDLEAL
                     VRRAAPFAELPRDALEGVLDMLSGRYPSDEFAELRPRLNWDRVAGGLEPRPGAQRLAV
                     TSGGTIPDRGLFGVYIAGEKPVRVGELDEEMVYESRVGDVFVLGASSWRIEEITHDRV
                     LVSPAPGVPGRMPFWHGDMLGRPVELGRALGAYVRELWGLAPSARDARLREAGLDENA
                     RANLVRYLEEQHEATGVLPDDRTIVVERFRDELGDWRLCVHSPFGARVHAPWSQAIEA
                     RVRERLELEVQTMYTDDGIVVRLPEADEAPPGDSVLFDPEDVEDLVVGQVGSSALFAS
                     RFRECAARALLLPRRRPGQRTPLWQQRQRSANLLQVASRYGSFPVVLETYRECLQDVF
                     DLPGLTELMAAVRRRDVRIVEVETPFPSPFAGSLLFGYVAAFLYEGDAPLAERRAQAL
                     SLDRRLLAELLGRAELRELIDAGALAEVELELQRLAEGRKVRDADGLHDALRTLGDLT
                     LEEARERSADPTATGRWLGELEAAHRALRLRIAGEERWTAIEDAARFRDALGAALPTG
                     VPDSLLAPVDDPVADLVARYARTHGPFGAQEPAARLGL"
     gene            complement(3764..>4538)
                     /locus_tag="VEO00_11665"
     CDS             complement(3764..>4538)
                     /locus_tag="VEO00_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58690.1"
                     /translation="GIRLAADLPGFVLHCRAWRAASFARMGLWDEASEELARAEDLLG
                     ERRENPPPFADRLFGASAFVLDARGEPTAERYVLMLRRFMDTGIGAWGAMGWLIEILA
                     RRREFETARDLVRRHEASRHRVHDGLVLEARCDLVALEGTWDEAEALLERARATAAEC
                     GLRALAASADRLEGRAALAGGRPDVAVAALERARAAFDDLEAGWDAAVTTLSLAEAHA
                     AAGNEPEARAAATAALETFDRLGALRETAAARTLLDRLR"
BASE COUNT          634 a         1687 c         1642 g          575 t
ORIGIN      
        1 gagccccagg cgcgcggcgg gctcctgcgc tccgaacggg ccgtgtgtgc gggcgtaccg
       61 cgccaccagg tcggccaccg ggtcgtcgac cggcgccagg aggctgtccg gcaccccggt
      121 cggcagcgcc gccccgagcg cgtcccggaa ccgtgcggcg tcctcgatcg cagtccaccg
      181 ctcctcgccg gcgatccgga gccgcagcgc ccggtgggcg gcctccagct cgcccaacca
      241 acgcccggtc gcggtcgggt cggcgctgcg ctcacgcgcc tcctcaagcg tcaggtcgcc
      301 gagcgtgcgc agcgcatcgt ggagcccgtc cgcgtcgcgc accttccgcc cctcggccag
      361 gcgctgcagc tccagctcga cctcggccag ggcgccggcg tcgatcagct cgcggagctc
      421 ggcgcggccg agcagctcgg ccagcaggcg acggtcgagc gaaagggcct gggcccggcg
      481 ctcggcgagc ggggcgtcgc cctcgtagag gaatgccgcc acgtacccga acagcagcga
      541 tcccgcgaag ggcgatggga agggcgtttc gacctcgacg atccgcacgt cccgccggcg
      601 caccgccgcc atcagctcgg tgagcccggg gaggtcgaac acgtcctgca ggcactcgcg
      661 gtacgtctcg agcaccaccg ggaaggagcc gtagcgcgac gcgacctgca gcaggttcgc
      721 gctgcgctgg cgctgctgcc agagcggcgt ccgctgcccc ggccggcggc gcgggaggag
      781 aagcgcgcgg gcggcgcact cgcggaaccg gctggcgaac agcgccgagc tcccgacctg
      841 gccgacgacg aggtcctcga cgtcctccgg atcgaacagc accgagtcgc cgggcggggc
      901 ctcgtccgcc tccggcagcc ggaccacgat cccgtcgtcc gtgtacatcg tctgcacctc
      961 gagctcgagg cgctcccgca cccgggcctc gatcgcctgg gaccaaggcg cgtgcacgcg
     1021 cgccccgaac ggcgaatgga cgcacagtcg ccagtcgccg agctcgtcgc ggaaccgctc
     1081 gacaacgatg gtccggtcgt ccggcaggac gccggtggcc tcgtgctgct cctcgaggta
     1141 gcggaccagg ttcgcccggg cgttctcgtc gaggccggcc tcgcgcagcc gggcgtcgcg
     1201 agcggacggc gccaggcccc acagctcgcg cacgtaggct ccgagggcgc ggccgagctc
     1261 caccggccgc ccgagcatgt cgccgtgcca gaacggcatc cgcccgggaa cgccaggcgc
     1321 cggcgagacc aggacgcggt cgtgcgtgat ctcctcgatc cgccaggagg acgcaccgag
     1381 gacgaacacg tcgccgacgc ggctctcgta gaccatctcc tcgtccagct cgccgacgcg
     1441 caccggcttc tcgccggcga tgtacacgcc gaacaggccg cggtcgggga tcgtcccgcc
     1501 ggaggtgacc gccaggcgct gggcgccggg gcgcggctcg agcccgccgg ccacccggtc
     1561 ccagttcagc cgggggcgca gctcggcgaa ctcgtcggac gggtagcgac cggagagcat
     1621 gtccagcacg ccctccagcg cgtcccgggg cagctcggcg aatggggcgg cccgacggac
     1681 gagggcctcg aggtcgtcca cggtccaggg gtccatcgcc accatcgaga cgacctgctg
     1741 cgccaggacg tcgagcgggt tgcgcggaac cgacagtgcc tcgatggcgc ctgcacgcat
     1801 ccgttcgacg accaccgcgc actccaccag gtccccgcgg tacttcggga acaccgttcc
     1861 gcggctgacg gcgcccacct ggtgcccggc gcggccgatg cgctgcaggc cctgcgccac
     1921 cgacgccggc gcctcgacct ggatcaccag gtccaccgcg cccatgtcga tgccgagctc
     1981 gagcgacgag gtggcgacga ccgccgggag gcgaccggcc ttcaggtcgt cctcgatctg
     2041 cagccgctgg tcgcggctga ccgagccgtg gtgcgcgcgg gcgatcgggc cttccgggtc
     2101 ggcggcgtcc tcgcgcgcga tcgcgttcag ccgggcgcac agccgctcgg ccaggcggcg
     2161 cgagttggcg aacacgatcg tgctgcggtg cgcgcggatc aggtcgacga tctcgcgctc
     2221 gacggccggc cagacgctgg cgcgctcctc cggatcgccg cccggcccgc cgccgtcggc
     2281 cggaacctcc ccgcccggag cggtcatgtc ctcgaccggg acgaccacct tcaggtcgaa
     2341 ggtcttctcc gcgagcggct gcaggatctc gaccggccgg ccgcccgtca ggaagcggcc
     2401 gacctcgtcc acggggcgga cggtcgccga caggccgacg cgctgcgcgg ggcgctcgag
     2461 caactgatcg acccgctcca gggagagtgc caggtgagcg ccgcgcttgt tcccggcgat
     2521 ggcgtggatc tcgtcgacga tcaccgtctc gacgttcgcc agcgcctcgc gcgcctgcga
     2581 ggtgaggatc aggaagagcg actcgggcgt cgtgatcagg atgtcgggcg gccgggtggc
     2641 gaagcggcgg cgatcctcgg ccggcgtgtc gccggtccgc accgcgaccg ccacgtcggg
     2701 aaccgcctgg ccgaggcgct ccgcggccgc gcgcatgccg gagagcggcg agcgcaggtt
     2761 ccgctcgacg tccacggcga gggccttcag cggtgagacg tacaggacgc ggaggcgctt
     2821 cttctgctcc ggggcggggc tcgtggcgat gcggtcgagc gcccagagga acgcggcgag
     2881 cgtcttgccg gacccggtcg gcgcgacgac gagggcgtcc cgtcccgtcg agatcgcctc
     2941 ccaggcctct gcctgagccg gcgtgggctc ggcgaacgcc gactcgaacc agccgcgcgc
     3001 gagaggcgag aaccgatcga gggggctcac cgcccgatgc taccccggcc ctccgacaat
     3061 gccgccgggc tacgggccgg tcgcgcgtcg gttctctgca tcggagcccc tggtgaacgg
     3121 ctcggtgccg tcagggactg gcgggggagg ctcgctggga ggtggatctt caggcatctc
     3181 ctcgtcgcgc aggacaagag cgtccatctc ggctgcgacg tgcttgcgga tccgagtgtc
     3241 tccaaggagg tcatcgatcc atcgcccgag gcctttcggg tgatcggtcc catcaggtac
     3301 cggacggggg agcttggtct cgatctcgac gtgcggggcg acacgccgtt gttgggtggc
     3361 catcgatcac cctccgggac gtccgcccag gatcgtccag atgaagtatg cgcctatcac
     3421 cacgacaccg acgaagagaa ggaccaggct cagggtccgt atcggcgagt ggcgaacgca
     3481 atgaggccaa gggcgaggga tccgatgatg gcccctgcgg ccaccactcc gatgatctgc
     3541 ggtgcctccc tcggcaggga atcgcccgcg agcaagagtc ctccgatcac accgtcggcg
     3601 gcgatcaaga tccccgccct ccgttcagcg cttcgacccg acggaacggt tcgtcaaacg
     3661 atcggattcc ggtctcgaag gcaaatgcag caccgggttg gggtggttct ggctgcgaca
     3721 tggtcgaacg gtagcgaccg ccacctacat cgaaccggtc gggtcagcgc agccggtcga
     3781 ggagcgtgcg ggcggcggcg gtctcgcgga gagcaccgag gcggtcgaag gtctccaggg
     3841 cggccgtcgc ggcggcgcgg gcctcgggct cgttcccggc ggcggcgtgg gcctcggcca
     3901 gggaaagggt ggtgaccgcc gcgtcccagc cggcctcgag gtcgtcgaag gcggcccgag
     3961 cgcgctcgag cgcggccacg gcgacgtcgg ggcggccccc ggcgagcgcg gcccggccct
     4021 ccaggcggtc cgcagacgcg gccagggcac gcagcccgca ctccgcggcc gtcgcgcggg
     4081 ctctctcgag cagcgcctcg gcctcgtccc acgtcccctc gagcgccacc aggtcgcacc
     4141 gggcttcgag cacaaggccg tcgtggaccc ggtggcgcga cgcttcgtgc cggcggacca
     4201 ggtcccgggc cgtctcgaac tccctccgcc gcgccaggat ctcgatcagc caccccatcg
     4261 cgccccaggc cccgatcccg gtgtccataa agcggcggag catcaagacg tagcgctcgg
     4321 cggtcggttc cccccgcgcg tcgagcacga aagccgacgc cccgaacagg cgatccgcga
     4381 acggtggcgg gttctcccgg cgttctccca gcaggtcttc cgcccgcgct agctcctcgc
     4441 tcgcctcgtc ccacagcccc attctcgcga aggacgcggc cctccaggcc cggcagtgca
     4501 ggacgaagcc ggggaggtcg gcggcgagcc ggatcccg
//
LOCUS       DASPWO010000508         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_495072, whole genome shotgun sequence.
ACCESSION   DASPWO010000508 DASPWO010000000
VERSION     DASPWO010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495072"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VEO00_11670"
     CDS             complement(<1..246)
                     /locus_tag="VEO00_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58691.1"
                     /translation="MTGPRPEDEHYTLLDAFADDPGTPAPVARRAREWRTFVRFGAWE
                     VANPESAPGVLLEDLVTGRFVFAAVPPEQLEHLPRWSV"
     gene            complement(243..1085)
                     /locus_tag="VEO00_11675"
     CDS             complement(243..1085)
                     /locus_tag="VEO00_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HYU58692.1"
                     /translation="MADRALEEAASKVRAMLDPETPPAEVAAHFLDMYEDDRLAPHGF
                     SLALGAGVEGKAAEVADEALRRAPGTVTALALAADVAEVAGDLEEAEERYREAFGVLA
                     LAASGDRGDDDEPAWARPGGHDPAAGDDEDGGSSTRVALSTRLAGVMAERDRVADALD
                     LLFDVGREDPGYAPAHPFLSAALAVASERRSMDPAEPCPCGSGRTHGECCRPREAAAL
                     ARFEDRSPMYEGRDALRPFLERPEIDPLVMDGISDWYLRRTPEEMATRGRPRSGSPSN
                     GSWW"
     gene            complement(1520..>2090)
                     /locus_tag="VEO00_11680"
     CDS             complement(1520..>2090)
                     /locus_tag="VEO00_11680"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HYU58693.1"
                     /translation="MAFLVLLENLTPVERAVFLLREVFDYDYAEIAQVVGKTEDNCRQ
                     IAVRARRRIEDGRPRFEADRRRRDELAQYFWAAARNGDTESLIDLLAEDVVLYGDGGG
                     QVAGAFRHAIHGKATIGKLIGPLLQVTKKRGWTMHPAEVNGQPGCVWVDEAGDPMVVV
                     SLDIAEDKIQTIRSVANPDKLRHLQQAAR"
BASE COUNT          260 a          778 c          730 g          322 t
ORIGIN      
        1 gaccgaccac cggggcaggt gctcgagctg ctcgggcggc acggccgcga acacgaaccg
       61 ccccgtcacg aggtcctcca gcagcacccc gggggccgac tccggattcg cgacctccca
      121 cgccccgaac cggacgaacg tccgccactc ccgggcccgc cgggccaccg gagccggcgt
      181 ccccgggtcg tcggcgaacg cgtcgagcag ggtgtagtgc tcgtcttccg ggcgcgggcc
      241 cgtcaccacc aggagccgtt cgacggcgag ccggacctcg gtctcccccg ggtcgccatc
      301 tcctcgggcg tccggcggag gtaccagtcg gagatcccgt ccatgacgag cggatcgatc
      361 tccggtcgct ccaggaacgg gcggagcgcg tcgcggccct cgtacatggg cgagcggtcc
      421 tcgaacctgg ccagcgctgc ggcctcgcgc gggcggcagc actcgccgtg cgtgcggccc
      481 gacccgcagg gacagggctc cgcgggatcc atggaccgcc gctccgacgc gacggccagc
      541 gccgcgctca ggaaggggtg cgcgggcgcg tacccagggt cctcccggcc cacgtcgaac
      601 agcaggtcga gggcgtccgc gacgcggtcc cgctcggcca tgacaccggc caggcgggtg
      661 ctcagggcga cgcgggtgga cgaaccgccg tcctcgtcgt ccccggccgc cgggtcgtgg
      721 ccgcccggcc gggcccacgc cggctcgtcg tcgtccccgc gatcgccgct cgccgcgagg
      781 gccaggacgc cgaacgcctc ccggtaccgc tcttcggcct cctccaggtc gccggccacc
      841 tcggcgacgt cggccgccag ggccagcgcc gtgaccgtcc ccggcgccct ccggagggcc
      901 tcgtccgcga cctcggcggc cttcccctcc acgccggccc cgagggccag cgagaacccg
      961 tggggcgcga gccggtcgtc ctcgtacatg tcgaggaagt gcgcggccac ctcggccgga
     1021 ggcgtctccg gatcgagcat ggcccggacc ttcgaggcgg cctcctcgag ggcccggtcg
     1081 gccatggtgc gcctcgcgcg ggaccgttcg cgctggaccg cctcgtggtg ctgcctggtc
     1141 ctggttctct gcttccttcg ccggttcgtg gtcatggcag agcgagctcc gatccgggga
     1201 aggttcgatc tcgagtctcc ggcgccgccc gtgggcgggc gcaggtcatg atacctaccc
     1261 ccgcggacat ccggaaagac ggcccgatcc ctgtcggggc ctcggcatat ggtcgctcgc
     1321 atccccctcc agccccgctt ggcgcgcgtg aggaggagga ggacaatggc ggtcgatgaa
     1381 cgggccaggc acgagctgtt cctcagggtc gagggcgagc tcggtcccca atgatgctct
     1441 gcgcgagcct ggtgttcgcc gtggccggcc tggcgttcgc cgcggccagg ctctcgtagc
     1501 cgcgtcgcgg atcagctcgt cagcgcgcgg cctgctggag gtggcggagc ttgtcggggt
     1561 tcgcgactga gcggatcgtt tggatcttgt cttcggcgat gtcgaggctc acgaccacca
     1621 tgggatcgcc ggcctcgtcg acccagacgc aaccgggctg gccgttgact tcggctgggt
     1681 gcatggtcca gcctcgcttc ttggttactt ggagcagggg gccgatgagc ttgccgatcg
     1741 ttgccttgcc gtggatggcg tgccgaaacg ctcctgcgac ctggcctccg ccgtcaccgt
     1801 agaggaccac gtcttcggct aggaggtcga tcagcgactc cgtgtctcca ttcctggcgg
     1861 ctgcccagaa gtactgggcc agctcgtccc gtcgccggcg gtcggcctcg aacctcggac
     1921 ggccgtcctc gatccgcctt ctcgcccgca ccgcgatctg gcggcagttg tcttcggtct
     1981 tgccgaccac ctgggcgatc tccgcgtagt cgtagtcgaa gacctctctc agcaggaaca
     2041 ccgctcgctc gaccggcgtg aggttctcca gcagcaccag gaacgccatc
//
LOCUS       DASPWO010000509         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_839575, whole genome shotgun sequence.
ACCESSION   DASPWO010000509 DASPWO010000000
VERSION     DASPWO010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839575"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..146
                     /locus_tag="VEO00_11685"
     CDS             39..146
                     /locus_tag="VEO00_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58694.1"
                     /translation="MAGSRNPKHVRDNAAAGDIDLDEATLAEIAKILRD"
     gene            479..724
                     /locus_tag="VEO00_11690"
     CDS             479..724
                     /locus_tag="VEO00_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58695.1"
                     /translation="MSQKSARNVADRLRASSPSQFWFGLMALASSVIFFIGNLSQGNV
                     IWATVFAVTFALLSVLMISAVVLKRQDRRHQRNSQVP"
     gene            complement(737..1609)
                     /locus_tag="VEO00_11695"
     CDS             complement(737..1609)
                     /locus_tag="VEO00_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018831357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYU58696.1"
                     /translation="MRRERGIWLLVAPYLLGLTVLVIAPVAVTFSLSLFDYDLIRPAV
                     FRGLANFQELARDETFRTSLWNSALFAAWAVPLRLLGALGLALLLHRRYRAVGAYRSA
                     VYLPTVIPDVAYGLVWLWILNPLYGPLNLVLGAAGLSTPSWLTEPATAQAAVIVMSLF
                     TIGEGFVVALAVRQQVPHDLYEVAVLEGAGSLRTFRTITLPLMAPALLLLLFRDAVYS
                     FQVSFVPALVVTHGGPPPSSTTFLPLFVYREGFTHLRYGYAAAASLAMLAVTALIVAI
                     QWAIIRRWRRTFTP"
     gene            complement(1609..>2251)
                     /locus_tag="VEO00_11700"
     CDS             complement(1609..>2251)
                     /locus_tag="VEO00_11700"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HYU58697.1"
                     /translation="ALARALVRDPDVMLLDEPLGNLDAQQRVAMRAELDRFHREVGAT
                     MLFVTRDQTEALTLGERVAVVQEGALQQVGTPDEVYRRPVNRFVASFIGSPPMNLLPA
                     TVDGGVLRAGPFVIPLPGSMQRLAGRALELGIRPEDLEVSLTATGAPARVEAVEVAGP
                     EAYLRCTASGLPLVARVGHDVRPSAGGIVHVDFERERAYLFDVLTGRTLVYAD"
BASE COUNT          374 a          787 c          805 g          285 t
ORIGIN      
        1 tcgcatggtg cgtccagcaa cccgggatca cggtagccat cgccggcagc cgcaacccca
       61 agcacgtccg cgacaacgcc gccgccggcg acatcgacct cgacgaagcc accctggccg
      121 agatcgccaa gatcctccgc gactagctcg ctgcccgaga tgccattgtt cctcccgccc
      181 cgggagaccc tcgcgcccgg ggtggcggcg gggcgcgctc ctacggtcgg gcggccgaat
      241 tcgctccggg ctgcgctcgc tccgcgtgtc tctcccggga gccgtgactc gcttggaggc
      301 gggctgacgc gtccgaggct ccgctcgact ccgccctgcg ctcgccggct tcgccggcct
      361 gcggccgccc tccccggagc gcatccccgc cgccgaccgg aagacgactc gaagcacgct
      421 cgaccaaccg cttgacacgt aagtagccag cctcgtcgat gcgatgtatg actcgaccgt
      481 gagccagaag tcggcacgca atgtcgctga cagattgcgg gcgtcctcgc caagccaatt
      541 ctggttcggc ctgatggcgc tcgcgtcgag cgtgatcttc ttcatcggca atctgagtca
      601 gggaaacgtg atctgggcaa ccgtgttcgc tgtcactttc gcgctgttgt ccgtgctgat
      661 gatctcggcg gtcgtcctga aacggcaaga ccgtcgacac cagcggaaca gtcaagttcc
      721 ttgacgatct ctccgttcag ggggtgaagg ttcggcgcca gcggcggatg atcgcccatt
      781 ggatggcgac gatcagggcg gtcacggcca acatcgccag ggatgcagcg gcggcgtagc
      841 cgtagcggag gtgggtgaag ccctcgcggt agacgaagag gggaaggaag gtggtcgacg
      901 acggcggggg gccgccatgg gtgacgacga gggcgggaac gaagctcacc tggaacgagt
      961 agacggcgtc gcggaacagg agcaggagca gggccggggc catcagcggg agcgtgatcg
     1021 tgcggaaggt ccgcagcgac ccggcgccct ccaggacggc gacctcgtag agatcgtgcg
     1081 gcacctgctg gcggaccgcc agcgccacga cgaacccctc gccgatcgtg aacaggctca
     1141 tcacgatcac ggccgcctgc gccgtggccg gctcggtgag ccaggacggc gtggacagcc
     1201 cggccgcgcc gagcaccagg ttcagcggcc cgtagagcgg gttcaggatc cagagccaca
     1261 cgagcccgta cgccacgtcg gggatcaccg tgggcaggta gacggccgag cggtacgcgc
     1321 cgacggcccg gtagcggcgg tgcagcagga gcgccagccc gagggcgccg agcagccgga
     1381 gcgggacggc ccaggccgcg aacagggccg agttccagag cgaggtgcga aaggtctcgt
     1441 cgcgggccag ctcctggaag ttcgccaggc cccggaacac cgccggacgg atcaggtcgt
     1501 agtcgaagag cgacagcgag aacgtgaccg cgacgggggc gatgaccagg acggtcaggc
     1561 caagcaggta cggcgccacc agcagccaga tcccgcgttc ccggcgcatc agtcggcgta
     1621 gacgagcgtc cggccggtca ggacgtcgaa gaggtaggcg cgctcgcgct cgaagtcgac
     1681 gtggacgatg ccgccggccg acggacggac gtcgtggccg acccgcgcca cgagcggcag
     1741 acccgacgcg gtgcagcgca ggtacgcctc gggcccggcg acctcgaccg cctcgacgcg
     1801 cgccggggcc ccggtcgccg tgagcgacac ctccaggtct tccggccgga tgcccagctc
     1861 cagcgcgcgg ccggcgaggc gttgcatcga gccgggcagg gggatgacga acggcccggc
     1921 ccgcagcacc cccccgtcga ccgtcgccgg cagcaggttc atcggcgggc tgccgatgaa
     1981 cgacgcgacg aaccggttca cgggacggcg gtagacctcg tcgggcgtgc cgacctgctg
     2041 cagcgccccc tcctgcacca ccgccacccg ctccccgagc gtgagggcct cggtctggtc
     2101 gcgcgtgacg aacagcatcg tcgcgccgac ctcgcggtgg aagcggtcga gctccgcccg
     2161 catcgcgacc cgctgctgcg cgtcgaggtt cccgaggggc tcgtcgagca gcatgacgtc
     2221 cgggtcgcgg accagcgccc gcgccagcgc c
//
LOCUS       DASPWO010000510         2922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1002883, whole genome shotgun sequence.
ACCESSION   DASPWO010000510 DASPWO010000000
VERSION     DASPWO010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002883"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..433)
                     /gene="cutA"
                     /locus_tag="VEO00_11705"
     CDS             complement(107..433)
                     /gene="cutA"
                     /locus_tag="VEO00_11705"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HYU58698.1"
                     /translation="MGVRLVQLVTSLPARDDAVALARAAVEARVAACGQVVGPVTSAY
                     RWEGAVRGEEEHLLLLKVPVEGVDALAAFIRERHPYDTPELTALDGPFIDERYLAWAR
                     AETVEA"
     gene            778..1317
                     /locus_tag="VEO00_11710"
     CDS             778..1317
                     /locus_tag="VEO00_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58699.1"
                     /translation="MRRSFVVLAAMLAALALLATQALAADITGTPGNDYLYGTGGNDN
                     INGLAGHDWIWGNGGGDIIIGSYGFDKLIGNDGPDHLYGRSGNDVLWGEELGCGYFGG
                     GGFPCPSPDLDQFYGGTNNDVIYADDSAFLVADPTLGEFIDCGGGLDTAYVDPSDYAR
                     ALVGQSNLFTTCEAYVVSP"
     gene            complement(1701..1773)
                     /locus_tag="VEO00_11715"
     tRNA            complement(1701..1773)
                     /locus_tag="VEO00_11715"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1738..1740),aa:Ala,seq:tgc)
     gene            1991..>2922
                     /locus_tag="VEO00_11720"
     CDS             1991..>2922
                     /locus_tag="VEO00_11720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU58700.1"
                     /translation="MALCTFEGARFLEEQLDSILGQTVLPDELVVCDDGSSDWTVDIL
                     QAFRDVAPFPVRIERNPRLLGSTANFGKAIGLCSEQVIVLCDQDDVWLPLRLEITADV
                     FGRRPDLGLFFSDAELIDDRSRPTGERLWWRLGIRPAAIGAYLSGDRIDRVEQLTWGN
                     LVTGATAAFRTSFRDLVLPIPDGWVHDAWIALILSASAPVEASTVPLVRYRVHPAQQI
                     GIHSPGEGKRRTQLVRRARKRERYLMRRQSALLATAAERLRAAGLERPPEPGLLEYLE
                     GKARHFAVRGAIDRPGRLATIAREVRAGGYRRFS"
BASE COUNT          423 a         1075 c          995 g          429 t
ORIGIN      
        1 gggtgcacga gcagcgcgtt cgacgcgtgc gtcccgaggt ccgggaagcc gatcgcggac
       61 agcccggcgc gcgggctcgg cttccttccc cgggtgtccg cgccggtcaa gcctcgacgg
      121 tctcggcccg ggcccaggcc aggtagcgct cgtcgatgaa ggggccgtcc agcgcggtca
      181 gctcaggggt gtcgtacgga tgccgctcgc ggatgaaggc cgccagcgcg tcgacgccct
      241 ccaccggcac cttcagcagc aggaggtgct cctcctcccc tcggacggcg ccctcccagc
      301 ggtaggcgct cgtgaccggc ccgaccacct gtccgcacgc cgccacccgc gcctccacgg
      361 ccgcccgcgc cagcgcgacc gcgtcgtcgc gggccggcag ggacgtgacg agctggacca
      421 gcctgacccc cacggccctg cagcatatgg cgttgcacac cgacgctcgg cgagttggtg
      481 gagacgaccg gactcgaacg gcgacccccc tcttgcaaag gcgtcggtgc acgtttctcg
      541 gagtgcggca aagacgagaa gaacagggcg tccgccggct taataagtga acgctggccc
      601 atcacagcta cttctccttt agccgacgaa gatgaagcca ggggggtaga ttagtgtcac
      661 ccggcccact gggccaagga ctgccccggg atcgccaacc tcttgtgccg cggcgtgcgg
      721 cggcgcacac tacgcgcatc gcggccgtcg gccgcacgat cggaagcgag ggttccgatg
      781 cgcagatcct tcgtcgtgct ggcggcgatg ctggccgcgc tcgccctgct ggcgacccag
      841 gccctggccg ccgacatcac cggcaccccc ggcaacgact acctctacgg cacggggggc
      901 aacgacaaca tcaacggcct ggccggccac gactggatct gggggaacgg cggcggcgac
      961 atcatcatcg ggtcctacgg gttcgacaag ctgatcggca acgacgggcc cgaccacctc
     1021 tacggccgtt ccggcaacga cgtgctgtgg ggcgaggagt tgggctgcgg gtacttcggt
     1081 ggcggcggtt tcccgtgccc ctccccggac ctcgaccagt tctacggcgg caccaacaac
     1141 gacgtgatct acgccgacga cagcgcgttt cttgtggccg atccgaccct cggcgagttc
     1201 atcgactgcg gcggcgggtt ggacaccgcc tacgtggacc ccagcgacta cgcccgcgcc
     1261 ctggtgggac agagcaatct attcacgacc tgcgaggcat acgtcgtctc cccctgagcg
     1321 agcgagacgg gacggacggg gccggacaac cgtccccgtc cgttgaacga ggagactcga
     1381 cgaggctcgt gaccgaggat cgcgacgtca gccggtagcc ggcccacaca tcgcggcggc
     1441 cacgaaatcc tgagcgcgag ctccgtccga ctgatccgag gcatccgcac ctcggcttcc
     1501 tcctgctcgc gcagtcgctc ctcgacgatg aggaggggcg tggcagggaa gctgaccagg
     1561 gtcggacgcc cacgttcgtt cctgctggtg ccgctcacgt tcccgacaat cccctctcgg
     1621 cgtgctccgc cctggcagca agaccggcag caagccccgg cccgcacgcg cgggtctttg
     1681 tgaagggctg gtcagcgccg tggagacgac cggactcgaa ccggcgaccc ccgccttgca
     1741 aaggcggtgc tctcccaact gagctacgtc cccgcagcga ttgtaggcgg cggccccggc
     1801 gggggaaacg gtcccgtttg ttgcgaccgt cctggttccc tatcgttccg ggggcttcgg
     1861 aaggaggagt ccactgacca ccgacctctc ggcggcgccc cgcgtcgtcc ctgccgagct
     1921 gcgggatcgc ctcgacgagc ggttccggca cgcgctgcag gggcgccggg cgggcggact
     1981 gtcgctctcg gtggcgctgt gcaccttcga gggggcgcgg ttcctcgagg agcagctcga
     2041 ctcgatcctc ggccagaccg tgctccccga cgagctggtc gtgtgcgacg acgggtcgag
     2101 cgactggacg gtggacatcc tgcaggcgtt ccgggacgtc gccccgttcc cggtccggat
     2161 cgagcgcaac ccgcggctgc tcgggtcgac ggcgaacttc ggcaaggcga tcggcctgtg
     2221 ctccgaacag gtcatcgtgc tctgtgacca ggacgacgtg tggctcccgc tccgcctcga
     2281 gatcaccgcg gacgtcttcg gccgccggcc cgacctcggc ctgttcttct ccgacgccga
     2341 gctgatcgac gaccgctccc ggccgaccgg agaacggctc tggtggcgcc tcgggatccg
     2401 gccggcggcg atcggcgcgt acctgtccgg ggaccggatc gatcgggtcg agcagctcac
     2461 gtggggcaac ctggtcaccg gagcgacggc ggcctttcgc acgtccttcc gcgacctggt
     2521 gctcccgatc cccgacggct gggtccacga cgcctggatc gccctgatcc tgtccgcctc
     2581 tgccccggtg gaggcctcca ccgttccgct ggtccgctac cgggtccacc cggcccagca
     2641 gatcggcatc cactcgccgg gcgaggggaa gcgccggacc cagctcgtcc gtcgcgcccg
     2701 caagcgggag cgctacctga tgcgccggca gtcggccctg ctggccaccg ccgccgagcg
     2761 cctccgcgcc gccggcctgg agcggccgcc ggagcccggc ctgctcgagt acctggaagg
     2821 caaggcccgg cacttcgcgg tccggggcgc gatcgaccga cccggccggc tcgccacgat
     2881 cgcccgcgag gtccgcgccg gcggctaccg ccgcttctcg aa
//
LOCUS       DASPWO010000511         3294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_495245, whole genome shotgun sequence.
ACCESSION   DASPWO010000511 DASPWO010000000
VERSION     DASPWO010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495245"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..389
                     /gene="rpmA"
                     /locus_tag="VEO00_11725"
     CDS             135..389
                     /gene="rpmA"
                     /locus_tag="VEO00_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HYU58701.1"
                     /translation="MAHKKGGGSSRNGRDSESKRLGVKRFGGQQVTAGSILVRQRGTK
                     IHPGAGVGKGGDDTLFARSAGVVTYHSSNGRRFVSITPAE"
     gene            complement(394..813)
                     /locus_tag="VEO00_11730"
     CDS             complement(394..813)
                     /locus_tag="VEO00_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58702.1"
                     /translation="MPEPRTVWLVEPPRNVDGVEGTLMLEHDPARLVFAAREPAGSLT
                     IPFDRLQGVRRIRGTPILEVRHRARGIDQEVFLYFTSPPPVPGRPQPTLPGSTGRARR
                     RERRKALTWLWKRNATLKGVVQAWADAIEGSRRPGGA"
     gene            906..2159
                     /gene="obgE"
                     /locus_tag="VEO00_11735"
     CDS             906..2159
                     /gene="obgE"
                     /locus_tag="VEO00_11735"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HYU58703.1"
                     /translation="MAFVDEARVSARGGHGGGGAATFRQEPYTPRGGPDGGDGGRGGD
                     VVLEVSRDVYDLSALADARELRAGDGGAGGRNRRSGAGGKDLVVLVPDGTVVHDERGL
                     VADLVGLRARAMVAHGGRGGRGNAALANAANRLPRVAERGEAGEEHLLELEIRLVADV
                     GLVGLPNAGKSTLLAALTAARPRIADYPFTTIDPNLGVAEVGDLRFVVADMPGIVEGA
                     SEGRGLGRKFLKHIARCRALVCVVEATAADPRGDLRQVLGEIEGYDRELAARPRLIVV
                     TKADLLTPGAAVPAVLAGQVAISAVTGQGLDELRERLATLVGEAAAAAPSREPYVVLR
                     PGRLPFEVLREGERFRVRGQRVERWVAETDLDDPRQVAALQKRLVRAGVERQLAAAGA
                     RRGDEVVIAGRAFEFIPEEMAEGEG"
     gene            2156..3271
                     /gene="proB"
                     /locus_tag="VEO00_11740"
     CDS             2156..3271
                     /gene="proB"
                     /locus_tag="VEO00_11740"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HYU58704.1"
                     /translation="MTVRVIRPLPAIGRVVVKVGTSSLVTERGGVSRGRLDRVVTEVA
                     VAARDRSCVLVSSGAIAAGLRPLGMTKRPSEVPALQAAAAVGQGRLMAAYAEAFARRR
                     LVAAQVLLTQDDFVHRRQFVNARNTLRRLLESAAVPVVNENDTVATEEIRFGDNDRLA
                     ALVAVMIEADLLVLLSDVDGIFSRDPRQGDAVLLSELSDADEITAGGPHRGSGGMASK
                     LEAARIATAAGIGVVVANAGRQRVLARILAGEPEGTYLPPAPKRQRARKAWIAFVRDV
                     RGRVVVDEGAAKAVREDGRSLLAAGVRAVEGSFAAGDAVEVAGPDGVAFARGIVNYGA
                     AELPKLLGRTTADLASLAGGPYDTEVIHKDQLVVIEA"
BASE COUNT          422 a         1168 c         1283 g          421 t
ORIGIN      
        1 cggcgccgcc cggcaccgtc atgatcccgg gcagcgccgg cccgtcgatc cggacctcca
       61 catcccgctc gtccattcgg catcccttcg agcgtcgact ggctttcagg agtctctgct
      121 accctcaatc gaccatggca cacaagaagg gcggcggctc ctcgcgcaac ggacgggaca
      181 gcgagtccaa gcggctcggc gtcaagcggt tcggcggtca gcaggtcacc gccggatcga
      241 tcctggtgcg ccagcgcggg accaagatcc atcccggggc aggggtcggc aagggcggcg
      301 acgacacgct cttcgcgcgc tcggcgggcg tggtgaccta ccattcctcg aacggccgcc
      361 ggttcgtctc gatcacaccc gccgagtagc cggtcaggct ccgccgggcc gccgagagcc
      421 ctcgatcgcg tcggcccacg cctggaccac gcccttcagg gtggcgttgc gcttccagag
      481 ccaggtcagc gccttgcgcc gctcccgccg ccgggcgcgg ccggtcgaac ccggcagcgt
      541 cggctgcggg cggcccggca ccgggggggg cgaggtgaag tagaggaaca cctcctggtc
      601 gatccctcgg gcgcggtggc ggacctccag gatcggcgtc ccgcggatac ggcgcacccc
      661 ctgcaggcga tcgaagggga tcgtcagcga tccggccggc tctcgtgccg cgaacacgag
      721 ccgcgccggg tcgtgctcga gcatcagcgt gccctcgacg ccgtccacgt tcctcggcgg
      781 ctccaccagc cagacggtcc gcggctcagg cacgttgcgg gattccggca cgccaccatc
      841 ttcgcccccc ggccacgtcc ggcgagcgaa cccccaagtg agaacgggcc ggtaccctgg
      901 aggtcatggc gttcgtggac gaagcgcggg tctcggcgcg gggcggccac ggcgggggcg
      961 gggcggcgac cttccgccag gagccctaca cgccgcgcgg cggcccggac ggcggagacg
     1021 gcgggcgcgg cggggacgtc gtgctcgagg tgtcgcgcga cgtctacgac ctctccgcgc
     1081 tcgccgatgc ccgtgagctc cgcgccgggg acggtggcgc cggcggccgc aaccgtcgca
     1141 gcggcgccgg cggcaaggac ctcgtcgttc tcgtgccgga cgggaccgtc gtccacgacg
     1201 agcgcggcct ggtggccgat ctggttggtc tccgcgcgcg ggcgatggtg gcgcacggcg
     1261 gccggggggg ccgggggaac gctgcgctgg cgaacgcggc caaccgcctg ccgcgggtgg
     1321 ccgagcgagg cgaggcgggc gaggagcacc tcctggagct cgagatccgc ctggtcgccg
     1381 acgtcgggct cgtcggcctc ccgaacgcag ggaagtcgac cctgctcgcc gcgctgacgg
     1441 cggcccgacc caggatcgcc gactatccgt tcacgaccat cgacccgaac ctcggcgtgg
     1501 ccgaggtcgg ggacctgcgc ttcgtggtgg ccgacatgcc aggcatcgtc gagggcgcca
     1561 gcgagggacg gggcctcggc aggaagttcc tgaagcacat cgcgcgctgc cgcgcgctcg
     1621 tgtgcgtcgt agaggcgacc gcggccgatc cgcgggggga ccttcgccag gtgctcggcg
     1681 agatcgaggg ctacgaccgc gagctcgctg cccgcccgcg cctgatcgtg gtgacgaagg
     1741 cggacctgct cacgccggga gcggcggtcc cggcggtcct ggccgggcag gtcgcgatca
     1801 gcgccgtgac cggccagggg ctggacgagc tgcgggagcg cctggcgacc ctggtgggcg
     1861 aggcggccgc ggccgcgccg tcccgcgagc cctacgtcgt gcttcggccg gggcgcctgc
     1921 cgttcgaggt gctgcgcgag ggcgagcggt tccgcgtgcg gggccagcgc gtcgagcgct
     1981 gggtggccga gaccgacctg gacgacccgc gacaggtcgc cgccctgcag aagcgcctgg
     2041 tccgggccgg tgtcgagcga cagctggcgg cggccggcgc gcgccgcggc gacgaggtcg
     2101 tcatcgccgg gcgcgccttc gagttcatcc cggaggagat ggcggagggc gagggatgac
     2161 cgtccgcgtg atccggccgc tcccggccat cgggcgggtc gtggtgaagg tggggacctc
     2221 cagcctggtc accgaacgag gcggcgtctc gcgcgggcgg ctcgaccgcg tggtgacgga
     2281 ggtggcggtc gccgcccgcg accggtcgtg cgtgctggtc tcgtcggggg ccatcgccgc
     2341 cggcctccgt ccgctcggca tgacgaagcg gccgtccgag gttccggccc tgcaggcggc
     2401 ggcggcggtg gggcagggac ggctgatggc ggcctacgcg gaggccttcg cccgccgccg
     2461 cctggtggcg gcgcaggtgc tgctgaccca ggacgacttc gtgcaccggc ggcagttcgt
     2521 caatgcccgg aacacgctgc ggcgcctgct cgagtcggcc gccgtcccgg tcgtgaacga
     2581 gaacgacacg gtcgccaccg aggagatccg cttcggcgac aacgatcgcc tggcggcgct
     2641 cgtcgccgtg atgatcgagg ccgacctgct ggttctgctc tccgacgtcg atgggatctt
     2701 ctcccgcgac ccgcggcagg gtgacgcggt acttctctcg gagctctccg acgcggacga
     2761 gatcaccgcc ggcgggccac atcgcgggtc gggcgggatg gccagcaagc tcgaggcggc
     2821 gcggatcgcc accgctgccg gcatcggcgt ggtggtggcg aacgccggac ggcagcgcgt
     2881 gctggcccgc atcctggccg gcgagccgga gggaacctac ctgccgccgg cgccgaagcg
     2941 ccagcgggcg cgcaaggcct ggatcgcctt cgtccgcgac gtgcgcgggc gggtggtggt
     3001 cgacgagggc gcggccaagg cggtgcgcga ggacggccgg agcctcctgg cggccggcgt
     3061 ccgcgccgtg gaggggagct tcgccgccgg cgacgcggtg gaagtggccg ggcccgacgg
     3121 cgtggcgttc gcccgcggga tcgtgaacta cggagcagcg gagctgccga agctcctcgg
     3181 gcggaccacg gccgacctcg cctcgctcgc gggaggcccg tacgatacgg aggtcatcca
     3241 caaggaccag ctggtcgtga tcgaggcgtg accatccccg acgaagcaaa cgag
//
LOCUS       DASPWO010000512         4153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1092693, whole genome shotgun sequence.
ACCESSION   DASPWO010000512 DASPWO010000000
VERSION     DASPWO010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092693"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VEO00_11745"
     CDS             complement(<1..433)
                     /locus_tag="VEO00_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58705.1"
                     /translation="MMLCEGSAESWDSWSGISKSIVDQLRADGHTVHVGDVDLYGAAR
                     WAAAAAALSRDRRRWTSRYHLGAVPFRLRSRSARRHVDAHRGRLDAIVQVGATFQTPN
                     SAGIPCYLCCDSNIRMAQHGASSGFSDAAALSDTDLNSIATR"
     gene            complement(456..914)
                     /locus_tag="VEO00_11750"
     CDS             complement(456..914)
                     /locus_tag="VEO00_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58706.1"
                     /translation="MREVYAGWAVKWSPNWACSPAGCGKIAFLRTEAEDLVYISYGDF
                     GLTSNPKSISVNTVWAPYGQRVWPANRALTTITPGTWYWIEWYNKYASSAAAGDGVMR
                     WWVNGVLNGDYEDVRFPSGGFVQFEFAPTLQNPPPGEQYMYVDHTHIGIR"
     gene            complement(930..2129)
                     /locus_tag="VEO00_11755"
     CDS             complement(930..2129)
                     /locus_tag="VEO00_11755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYU58707.1"
                     /translation="MISAPRDLAGLRIAHLIETDGPGGAERVLAHLATALQEAGALNV
                     AFLPAGGEGWLADQLKGSGVAIEYFRLKRPVSPACARWLEAALRRHRVAVAHSHEFTM
                     AVYGAWASRRAGIPHVITMHGNRYYVGRLRRRVALRAAIGLSGRAVAVSAPLASQLRR
                     DLWVPPSQIETIPNGVPHVPPERTTLRRELALRPDERLLVSVGNLYPVKGHRHLIDAL
                     ALLGPRHPRLHLAVAGRGGLENELLEQARNLGVGDRVHLLGLRSDVPAVLAAADLFVL
                     PSLSEGLPLALLEAMFAARPIVASDVGEVSVALAGGEAGALVPPGAPAPLAAAIDRLL
                     AAPKEASLLGEQAARRARAEYDVSVMVRRYLSLYERVMGRRRSPSVSQPASRLEPAPA
                     ESRTGDE"
     gene            complement(2126..3475)
                     /locus_tag="VEO00_11760"
     CDS             complement(2126..3475)
                     /locus_tag="VEO00_11760"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYU58708.1"
                     /translation="MARVTAPAPPAEACEPDRWWDLLLVCVAGHILTAVGRVHQIFPV
                     LGALHPAIITGILAIVLYLLDQRSERRFDRLWVATTKCLLALLGWMILSVPGALVDGN
                     SFDLVFDNFAKTVVLFLLTAGTIRGVCDVERLASVYLIGATAYAAVACTEFDLGPGAN
                     WRLGHLFYYDANDLATFLVTAVPFGLFFLQAARRTWSRVLAAGALALLMVAFVRTGSR
                     GGFIALLAVAGYVVLRYSAIPLRRRLGATVLVALVVAATASDQYWQQMGTIGSDTDYN
                     LTEESGRMQIWRRGIGYMLEHPMLGVGPGNFQAAEGMLSPIAGRQQFGVGVRWNAPHN
                     TFIQAGAELGVPGLVLFVAIIASAFVALRRSGSPERVGAGSADRQTQLAQVLTASLIG
                     FVAGAFFLSLAYSEMLYTLLALAIGLKKVSAPDTAAGATRRFRYRSAFPYRGAQEAR"
     gene            complement(3489..>4153)
                     /locus_tag="VEO00_11765"
     CDS             complement(3489..>4153)
                     /locus_tag="VEO00_11765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYU58709.1"
                     /translation="GCDVLVVPNGVDVDEFKPAAAMEEGLAFVGGANWFPNRDALAFF
                     CSEILPHLRAAKPSLPVRWIGAAPAELRKSHRERYGVELTGYVDDAIPLMREAACHVV
                     PLRAGGGTRLKILNSWAMGKAVVSTSMGCEGLAAADGVNILVRDDPKDFARAVLDVLA
                     DDLLRRRLGEQGRATAQRLYSWDVVGQPMIKAYLGLAGAPSGELATGTGTTHAEPGYG
                     YS"
BASE COUNT          688 a         1455 c         1451 g          559 t
ORIGIN      
        1 cccgcgtcgc aatggagttc aagtccgtat cgctgagcgc agcggcgtcg ctgaagcccg
       61 aggacgcgcc gtgctgagcc attcggatgt tcgaatcgca gcagagatag cagggaatcc
      121 cggcgctgtt cggcgtctgg aaggtggcgc cgacctgcac gatggcgtcg agccggcccc
      181 ggtgggcgtc gacgtgtcgt ctcgcgctcc gcgaccgaag ccggaagggg accgccccga
      241 gatgataccg gctcgtccaa cggcgccgat cacgcgagag cgccgccgcc gccgccgccc
      301 agcgggccgc tccgtagagg tcgacgtcac cgacgtggac ggtgtgaccg tcggcccgga
      361 gttgatcgac gatgctcttg gagatcccgg accaggagtc ccaggattcg gcgcttccct
      421 cgcagagcat caagatcctc atgcgacacc ggtcgtcatc ggatgccgat gtgcgtgtgg
      481 tcgacgtaca tgtattgctc cccgggaggc gggttctgga gcgtgggggc gaactcgaac
      541 tggacgaagc cgccgctcgg gaaccgtacg tcctcgtagt cgccgttgag gacgccgttg
      601 acccaccagc gcatcacccc gtcgccggcc gccgcgctgg aggcgtactt gttgtaccac
      661 tcgatccaat accaggtccc gggcgtgatg gtggtcaggg cccggttggc gggccagaca
      721 cgctgcccgt agggcgccca caccgtgttg accgagatcg acttcggatt ggaggtgagg
      781 ccgaagtcac cgtagctgat atacaccagg tcttcggcct cggtgcggag gaaggcgatc
      841 ttgccgcagc cggccggact gcacgcccag ttcggggacc atttcaccgc ccagccggcg
      901 tacacctccc tcaccgcggc ggtgggaggt cattcgtcac cggtgcgcga ctccgcgggg
      961 gccggctcga ggcgggacgc gggctgcgag accgatggcg aacgccgcct gcccatcacg
     1021 cgttcgtaga ggctgaggta gcggcggacc atcacggaaa cgtcgtactc cgcccgcgcg
     1081 cgccgcgccg cctgctcgcc gagcaggctc gcctccttcg gggcggccag gaggcggtcg
     1141 atcgcggcgg ccagcggcgc cggcgcgccc ggcggcacca gcgctccggc ctcgccgccg
     1201 gcgagggcga cactcacctc tccgacgtcg ctggccacga tcggccgggc ggcgaacatc
     1261 gcttcgagaa gcgcgagggg caatccttcc gacagcgacg gcagcacgaa caggtcggcc
     1321 gcggcgagaa ccgcgggaac gtccgagcgg agcccgagca ggtgcacccg gtccccgacg
     1381 ccgagattcc gcgcctgttc cagaagctca ttctccagcc cgccccgtcc ggcaacggcc
     1441 agatgcagcc ttggatggcg ggggccgaga agagcgagcg catctatgag atggcggtgt
     1501 cccttcaccg gatagaggtt gccaaccgag acgagcaggc gctcgtccgg ccggagcgcc
     1561 agctcgcggc gcagagtggt ccgttccggc ggcacgtggg gtacgccgtt ggggatcgtc
     1621 tcgatctgcg acggcggaac ccacaggtcg cggcggagct gactcgccag gggcgccgaa
     1681 accgccacgg cccggccgct caggccgatg gccgcgcgca acgccacgcg gcgccggagc
     1741 cggcccacgt agtagcgatt gccgtgcatc gtgatgacgt gcggaatgcc ggcccgccgg
     1801 gaggcccacg ccccgtagac ggccatcgtg aactcatggc tgtgggcgac ggcgacgcgg
     1861 tgccggcgca gggccgcttc cagccaccgc gcgcacgccg gcgagacggg ccgcttcagc
     1921 cggaagtact cgatggccac gcccgatccc ttgagctgat ccgccagcca cccctcgccg
     1981 ccggccggaa ggaacgccac gttaagggcc cccgcctcct gcaacgcagt ggccagatgg
     2041 gccaggacac gctccgcgcc gccggggccg tccgtctcga tcaggtgcgc gatccgcagt
     2101 ccggcgaggt cgcgaggagc ggagatcatc gcgcctcctg cgccccgcgg tatggaaatg
     2161 cgctccggta tcgaaatcgg cgcgtcgctc cggccgcggt gtccggcgcc gacaccttct
     2221 tcaggccgat ggccagagcc agcagcgtat acagcatctc cgaataggcc agcgacagga
     2281 agaacgcgcc cgcgacgaag ccgatcagcg aggcggtgag cacctgcgcc agttgcgtct
     2341 gcctgtccgc ggagccggct ccgacgcgct cgggggagcc cgaccggcgg agggccacga
     2401 aggcgctcgc gatgatggca acgaacagca cgaggcccgg cacgcccagc tcggcaccgg
     2461 cctggatgaa cgtgttgtgg ggggcgttcc atcgcacgcc gacgccgaac tgctggcgcc
     2521 ccgcgatggg cgacagcatt ccctcggccg cctggaaatt accgggcccg acgccgagca
     2581 tcgggtgctc gagcatgtaa ccgatgccgc gacgccagat ctgcatgcgc ccgctctctt
     2641 cggtcaggtt gtagtccgta tccgacccga tggtgcccat ctgctgccag tactggtcgc
     2701 tggccgtcgc ggccacgacc agcgccacga gcacggtggc gccgaggcga cggcgaaggg
     2761 gaatcgccga gtaccggaga acgacgtagc cggccaccgc gagcagggcg atgaacccac
     2821 cgcgggatcc ggtccgcacg aaggccacca tcagcagcgc gagggcgccg gcggccagga
     2881 cgcggctcca cgtccgccgg gcggcctgga ggaagaagag gccgaacggc acggcggtca
     2941 ccaggaaggt cgccaggtcg ttggcgtcgt aataaaagag gtgtccgagc cgccaattcg
     3001 ctccggggcc gaggtcgaac tcggtgcacg ccacggcggc atacgcagtc gccccgatga
     3061 ggtacaccga cgccagacgc tccacgtcgc aaacgccgcg gatggtgccc gccgtcaaga
     3121 ggaacagcac gacggtcttg gcgaaattgt cgaagacgag gtcgaagctg ttgccgtcca
     3181 ccagcgcgcc cgggacggac aggatcatcc agccgaggag cgccagcagg cacttcgtgg
     3241 tggcgaccca gagtctgtcg aacctgcgtt ccgagcgctg atccagaagg tagagcacga
     3301 tcgcgaggat cccggtgatg atcgcggggt ggagggctcc cagtacgggg aagatctggt
     3361 gaacgcggcc caccgcggtc aggatgtgcc cggcgacgca gaccagcagc agatcccacc
     3421 accggtccgg ctcgcaggcc tccgccggcg gcgcgggcgc cgtgacgcgc gccaacggcg
     3481 cggccgatct aagagtagcc atagcccggt tccgcatggg tcgtccccgt gccggtggcg
     3541 agctcgcccg atggagcccc cgccaggccc agataggctt tgatcatcgg ctgcccgacg
     3601 acgtcccagc tatagagccg ctgggccgtc gcccgaccct gctcgcccag ccgtcgtcgc
     3661 aggaggtcgt ctgccagcac gtccaggacg gcacgcgcga agtccttcgg atcgtccctg
     3721 acgaggatgt tcaccccgtc ggcggcggcc aggccttcgc agcccatcga ggtgctgacg
     3781 accgccttcc ccatcgccca cgaattcagg atcttcagcc gagtgccccc gccggcccgg
     3841 agcggcacca cgtggcacgc cgcttcgcgc atgagcggga tcgcgtcgtc gacgtagccc
     3901 gtcagctcca cgccgtagcg ctcccggtgg ctctttcgca gttcggccgg ggccgcgccg
     3961 atccagcgga cggggaggga cggcttcgcc gcccgcagat gaggcaggat ctcgctgcag
     4021 aagaaggcga gcgcatcccg attcgggaac cagttcgccc cccccacgaa ggcgagcccc
     4081 tcttccattg cggcggcggg cttgaactcg tcgacgtcga ccccgttcgg cacgacgagg
     4141 acgtcgcaac cgg
//
LOCUS       DASPWO010000513         8990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_330180, whole genome shotgun sequence.
ACCESSION   DASPWO010000513 DASPWO010000000
VERSION     DASPWO010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330180"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VEO00_11770"
     CDS             complement(<1..291)
                     /locus_tag="VEO00_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58710.1"
                     /translation="MERAGSPRTSNHRWGIHPRPPRYTMYGGVALFVWFAGWSVSGPA
                     PAPTPGSAVCTRAVKATTVAYLTGSACPPPGFASAFGYEPDLVRTPSGWRYTR"
     gene            complement(420..1559)
                     /locus_tag="VEO00_11775"
     CDS             complement(420..1559)
                     /locus_tag="VEO00_11775"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HYU58711.1"
                     /translation="MTSATRTAPSRPRPAPVRAKAAPPSRVRDALHLAVRLGLGGVFL
                     WAGLVKATHPQDTILAVDAYAVLPDGLVRVVAAVMPWAEIAAGALLLTGLFVRAAALG
                     TAVLSFAFLLGMAQAAIRGLDISCGCFGGTGAGEGVTWIEIARDLAFLAAAGYLFLRP
                     GGPLQADHRLDRPPRKLAVRRRMLAGRAIAVTVAAVLLVPASISVIDQVGDRSGAGHA
                     PSADQTVTIDGPARTTALQPGDRMPTFEAPGLTGGRSGYGASGPAQATVLIVWAPWCP
                     YCQKELPLVGRVAVDYPGVHVQTVVTAIGARPGPDPLSFLQENGLDFPTAVDDEAGTL
                     FNGFGIQNFPTSFYIGSDGVIVQTMVGATEEPDLRALFADLAALG"
     gene            1670..2251
                     /locus_tag="VEO00_11780"
     CDS             1670..2251
                     /locus_tag="VEO00_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HYU58712.1"
                     /translation="METERETRRGSLEIRLHGRGGQGTVTMAALLVDAAFRDGWHALG
                     FPAFGTERTGAPVTAFVRLSDEPIRDRSQIRTPRIVVVQDPTLAAAVDLTEGLEPGGT
                     VLVNAEIVPPRLRDLAGVTVIAVPVTALATEHLGSPITSTAMLGLLAGATGLVRIESV
                     CAAVRDRFSGSIGEKNEALVRAAHTLALGRKAA"
     gene            2248..2520
                     /locus_tag="VEO00_11785"
     CDS             2248..2520
                     /locus_tag="VEO00_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HYU58713.1"
                     /translation="MTADHGLPAGLVTRPGTSAVYQTAGWRTEIPRFLLAACNGCDRC
                     VAFCPEGIVVRLGKNRYGFDHDYCKGCGICAKECPVDDIVMEVEVR"
     gene            2517..3674
                     /locus_tag="VEO00_11790"
     CDS             2517..3674
                     /locus_tag="VEO00_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYU58714.1"
                     /translation="MRVMEGSEAIAEAVRVAVPKVIAAYPITPQTHVVEDLAKYVADG
                     RLDAEFLTVESEHSAASAVLGASAAGVRTFTATSSQGLLYMSEVVCAIAGMRLPVVMT
                     LANRAISAPLSIWNDHQDSMMLRDSGWVQIFAQTAQEAHDLHLVAYRVAEDTRVQLPV
                     MVCVDGFVLTHAFEPVEPLTEADAAALLPPYEPEVRLDPADPMTFGAYAEPSVYTEAR
                     YEVHDAMRVAGDVLTEALDAFAARTGRRYDPWFDRYRMEGAEVALIAMGTSAQTAQLV
                     VDDLRAEGVSAGLIALRTFRPFPAERLAEDLATVERLVVLDRSVSLGAGGIVAGEVRA
                     ALHAAGAPTRVIGVIAGLGGRDVSRETIRKAFEAEADGWVDLKHELVGSER"
     gene            3671..4612
                     /locus_tag="VEO00_11795"
     CDS             3671..4612
                     /locus_tag="VEO00_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYU58715.1"
                     /translation="MSQTLVEEAEVVHAPERAEHLIAPGHRACAGCGLLVGARLAADA
                     AGGNVVVTNATGCLEVVTTAFPGSAWRVPWIHSVFGNAAAVASGVKAALRASGRDDIP
                     VIAQAGDGGTADIGMQALSGMLERGDDVLFVLYDNEGYMNTGVQRSGLTPTDAATTTT
                     PAGRLSEGNPHLKKDLLAIVLAHRPVYAATATLAYHKDLQGKMGRAIAARGPRFLHVL
                     VPCPLGWRFKSESTIEVSRLAVLSNLFPLVEYRGGILDKVKRIGAPVPVDDYLRAQDR
                     FKHLFRDERGEVEREFLQSIADENARRLGLPVYRPAA"
     gene            4745..4951
                     /locus_tag="VEO00_11800"
     CDS             4745..4951
                     /locus_tag="VEO00_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58716.1"
                     /translation="MALRADDLRRDVLYRRYLQALARFGQPEEPAPAPGHAIRSCPTC
                     GRHTMFRLDPDGTWFECTRCGHYA"
     gene            complement(4973..6010)
                     /locus_tag="VEO00_11805"
     CDS             complement(4973..6010)
                     /locus_tag="VEO00_11805"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYU58717.1"
                     /translation="MTLPFKPTVAPMEAKVAADVPTGSDWRYEPKWDGFRSIVWRDGP
                     NVTITSRKQLPFNRYFPELEAAFAAVKIPDGVYDGEIVIFGKRGLDFNALLQRIHPAA
                     SRVRMLSETTPSSFVAFDLLAEGTEDLRGVGFAERRARLERAMKRAKTPLLLSPQTAD
                     PDEARGWLTEYDVAGLDGVVAKRGDRPYKSGEREMIKVKRQRTADCVVGGFRWYKNAH
                     GKAIGSLLLGLFHEGKLHNVGFTSGFSAKDRVEVAKVLKPHMGGAGFSGAGPGGPSRW
                     RSEEEAEYQPLDPVLVVEVWFDQVTGHRIRHGARFIRWRPDKPPESCTFEQLYPTGTT
                     EELNELFEGWA"
     gene            6267..6422
                     /locus_tag="VEO00_11810"
     CDS             6267..6422
                     /locus_tag="VEO00_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HYU58718.1"
                     /translation="MTRDDGPDVFVHFSAIEGAGYRNLEEGQTVEFEVTQGAKGDQAS
                     NVRIVGA"
     gene            complement(6542..7330)
                     /locus_tag="VEO00_11815"
     CDS             complement(6542..7330)
                     /locus_tag="VEO00_11815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HYU58719.1"
                     /translation="MTEAAFFDLDRTLIPGSSLFPIAREMFRRGYFTTGDIARFTLDQ
                     AAYRLFATEDPGRIRRARQGALDGIRERSREEVLELGRDLVRLELLPRMYPQALELVA
                     RHQRHGRQVYVCSAAPEDLLQLFAADLGLDGVLGTRALVDDDGRYTGELDGEPCHGEE
                     KARRVLDVADEQHLDLEGSYAYSDSVNDVPLFEVVGNPVATNPDRHLLQEARRRGWPV
                     LDFRAGRRRALVASVAGASAAATGVAAYVTGYLVGRARTRAAAA"
     gene            7385..7894
                     /gene="greA"
                     /locus_tag="VEO00_11820"
     CDS             7385..7894
                     /gene="greA"
                     /locus_tag="VEO00_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HYU58720.1"
                     /translation="MPERDPNHVPTAHLTRTALERLQAELHELKTSGRERMAERLQHA
                     REMGDIRENAEYDAAKNEQGLMEARIRTIERLLKDPVIVEKGEHSDEVAAGMVVTVLP
                     LDDGDGADEEEYLLAASAEERHPGARTVTTESPFGKALLGSRTGDTVRYAAPGGEFSY
                     RVIRFEPHG"
     gene            7943..8827
                     /locus_tag="VEO00_11825"
     CDS             7943..8827
                     /locus_tag="VEO00_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HYU58721.1"
                     /translation="MRTALPVDLPLSLGPLACGRWDPTIRADGGAWWRATRTATGPAT
                     VRYAPADGGVRVRAWGPGAEAALALAPDLLGARDELDGFDPGAGLVHELHRRFSGLRV
                     IRSRAVFQAIVPAVLEQQVTGKESRASWRRLVLRYGEPAPGPLAGLMLPPAAATLAAL
                     PYHAFHPLGVEMRRANTVRGVAAGAARLEATAAMPLADAHRRLRAFPGVGPWTAAVVA
                     GVALGDGDAVPVGDYHVKHMVSWALAGEPRGTDERMLELLEPFAGHRGRVVRLIKAAG
                     LAAPRFGPRMPARSYERL"
     gene            complement(8824..8982)
                     /locus_tag="VEO00_11830"
     CDS             complement(8824..8982)
                     /locus_tag="VEO00_11830"
                     /inference="COORDINATES: protein motif:HMM:NF018531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HYU58722.1"
                     /translation="MARRGHGRWVYRRADEPCRRCGTPIERIAQGPPAAERLTFLCPS
                     CQPLAADP"
BASE COUNT         1231 a         3264 c         3314 g         1181 t
ORIGIN      
        1 gcgcgtgtac cgccagccgg acggcgtgcg caccagatcc ggctcgtagc cgaaggcgct
       61 cgcgaagccg ggcggggggc aggcgctccc cgtcaggtag gccacggtcg tcgccttcac
      121 cgcgcgggtg cacaccgcgg aacccggtgt cggcgcgggg gcgggtcccg agacggacca
      181 tccggcgaac cacacgaaca gagcgacccc cccgtacatc gtgtagcgcg gagggcgcgg
      241 gtggatcccc caccggtggt tgctcgtccg tggcgacccg gcccgttcca tgtccggatg
      301 ctggagcgcc gagcggccgg cgaacagacg agaaagtgct caggtgctcc ttcacgagct
      361 cccgtttcgc ctgggcaccc ggcgtaggta gatgccgcag gtacgggtag ggtggcgcct
      421 cagccgagag cggcgaggtc ggcgaagagg gcccggaggt ccggctcctc ggtggcgccg
      481 accatggtct gaacgatcac gccgtcggac ccgatgtaga agctggtcgg gaagttctgg
      541 atcccgaagc cgttgaagag cgtcccggcc tcgtcgtcga ccgcggtggg aaagtcgagc
      601 ccgttctcct gaaggaagga caggggatcg ggcccggggc gcgccccgat cgcggtcacg
      661 accgtctgga cgtggacccc cgggtagtcc accgccacgc gcccgaccaa cgggagctcc
      721 ttctggcagt acggacacca gggcgcccag acgatcagta ccgtcgcctg cgccgggccg
      781 gaggcgccgt atccgctgcg cccgccggtc aggccgggcg cctcgaaggt gggcatccga
      841 tcgcccggct ggagcgcggt cgtccgcgcg gggccgtcga tcgtcaccgt ctgatccgcc
      901 gacggggcgt ggccggcgcc cgagcggtcg ccgacctggt cgatcaccga gatcgacgcc
      961 gggaccaaca gcaccgccgc caccgtcact gcgatggccc ggcccgccag catccgccgg
     1021 cgcacggcga gcttgcgcgg gggccggtcg agccgatggt cggcctgcag cggtccgcct
     1081 ggccgcagga acaggtagcc ggccgccgcc aggaacgcca ggtcgcgggc gatctcgatc
     1141 caggtgacgc cctcgccggc gccggtccca ccgaagcacc cgcacgagat gtcgaggccg
     1201 cggatcgccg cctgcgccat cccgagcagg aaggcgaacg acaggacggc cgtgcccagg
     1261 gccgccgccc ggacgaacag gccggtgagc agcaacgccc cggccgcgat ctcggcccac
     1321 ggcatgacgg ccgccaccac ccgcaccagt ccgtccggca gcaccgcgta ggcatcgacg
     1381 gcgaggatcg tgtcctgcgg gtgggtggcc ttcaccaatc ccgcccacag gaacacgccg
     1441 cccaacccga gccggaccgc caggtgcagg gcatcgcgca cgcgcgacgg cggagcggcc
     1501 tttgctcgga cgggcgccgg cctgggccgg gacggggcgg tgcgggtggc ggacgtcatc
     1561 gcactcgacg gtacggcccg cggggtgccc cgccggaggg ccggaggtcc cgcacggaag
     1621 gggccgctcg tccggtaccc gggtcccgcc ccgcacctac ggtgcggtca tggagaccga
     1681 gcgggagacc cgtcgtgggt cgctcgagat ccgtctgcat ggacggggtg ggcagggcac
     1741 cgtgacgatg gccgccctgc tcgtcgacgc cgcgttccgc gacgggtggc acgcgctggg
     1801 gttccccgcc ttcgggaccg agcggaccgg ggcgccggtg acggccttcg tccgcctgtc
     1861 ggacgagccg atccgggacc gcagccagat ccgcacgccg cgcatcgtgg tggtgcagga
     1921 cccgaccctc gccgcggccg tcgacctgac cgagggcctc gagccgggcg gcacggtcct
     1981 ggtgaacgcg gagatcgtcc cgccgcgcct gcgcgacctg gcgggggtca ccgtgatcgc
     2041 cgtgccggtc accgcgctgg ccaccgagca cctcgggtcg ccgatcacga gcaccgcgat
     2101 gctcgggctg ctcgcgggcg cgaccggcct ggtccggatc gaatcggtgt gcgccgccgt
     2161 ccgggatcgc ttcagcggct cgatcggcga gaagaacgag gcgctggtcc gggccgccca
     2221 cacgctggcg ctgggaagga aggcggcatg accgccgatc acgggctgcc ggccggactc
     2281 gtcacgcgtc cgggcacctc cgccgtgtac cagacggccg ggtggcggac cgagatcccg
     2341 cggttcctgc tcgccgcgtg caacggctgc gaccgctgcg tcgccttctg ccccgagggg
     2401 atcgtggtgc gcctggggaa gaaccgctac gggttcgacc acgactactg caaggggtgc
     2461 ggcatctgcg ccaaggagtg cccggtggac gacatcgtga tggaggtcga ggtccgatga
     2521 gggtgatgga gggctccgag gccatcgccg aggccgtccg cgtggccgtg ccgaaggtga
     2581 tcgcggccta cccgatcacg ccgcagaccc acgtggtgga ggacctggcg aagtacgtcg
     2641 ccgacggccg cctggacgcc gagttcctga cggtcgagag cgagcattcc gcggcgagcg
     2701 cggtcctcgg ggccagcgcg gccggcgtgc ggaccttcac ggcgacctcc tcgcagggcc
     2761 tgctctacat gtccgaggtg gtgtgcgcga tcgccgggat gcgcctgccg gtcgtgatga
     2821 ccctggcgaa ccgggcgatc tccgcgccgc tctcgatctg gaacgaccac caggactcga
     2881 tgatgcttcg cgactctggc tgggtgcaga tcttcgccca gacggcccag gaggcgcacg
     2941 acctgcacct ggtcgcctac cgcgtggcgg aagacacccg tgtgcagctg ccggtgatgg
     3001 tctgcgtgga cgggttcgtc ctgacccacg ctttcgagcc ggtcgagccg ctgaccgagg
     3061 ccgacgctgc cgccctgctg ccgccctacg agccggaggt gcggctcgat cccgccgacc
     3121 cgatgacgtt cggcgcctac gccgagccga gcgtgtacac ggaggcgcgc tacgaggtgc
     3181 acgacgcgat gcgagtcgcc ggcgacgtgc tgacggaggc gctcgacgcg ttcgctgctc
     3241 gcaccgggcg gcgctacgac ccgtggttcg accggtaccg gatggagggc gccgaggtcg
     3301 cgctgatcgc gatgggcacc tcggcgcaga ccgcgcaact cgtcgtcgac gacctgcgcg
     3361 cggagggtgt gtccgcgggg ctgatcgccc tgcggacgtt ccggccgttc ccggcggagc
     3421 ggctggccga ggacctggca acggtggagc gccttgtcgt cctcgaccgg tccgtctccc
     3481 tgggagcggg cgggatcgtg gccggggaag tgcgcgcggc gctgcacgcg gccggcgcgc
     3541 cgacccgggt gatcggcgtg atcgccggcc tgggcggccg cgacgtctcg cgggagacga
     3601 tccgcaaggc gttcgaggcc gaggccgacg gctgggtgga cctgaagcac gagctggtcg
     3661 ggagcgagcg gtgagccaga cgctggtcga agaagctgag gtcgtgcacg cgccggagcg
     3721 cgccgagcac ctgatcgctc ccgggcaccg cgcgtgcgcc gggtgcgggc tgctggtcgg
     3781 cgcgcggctc gccgcggacg ccgccggggg caacgtcgtg gtgaccaacg ccaccggctg
     3841 cctggaggtc gtcacgacgg ccttccccgg gtccgcgtgg cgggtgccgt ggatccactc
     3901 ggtgttcggc aacgcggcgg cggtggcgag cggcgtcaag gcggcgctgc gcgcgagcgg
     3961 ccgcgacgac atcccggtga tcgcgcaggc cggcgacggg ggaaccgccg acatcgggat
     4021 gcaggcgctc tcgggcatgc tcgagcgcgg cgacgacgtt ctcttcgtcc tgtacgacaa
     4081 cgagggctac atgaataccg gcgtccagcg ctccggcctg accccgaccg acgcggcgac
     4141 gaccaccacc ccggcgggac ggctctcgga gggcaaccca cacctgaaga aggacctgct
     4201 ggcgatcgtc ctcgcccacc ggccggtcta cgcggccacc gcgacgctcg cctaccacaa
     4261 ggacctccag gggaagatgg gccgcgcgat cgcggcgcgc gggccgcggt tcctgcacgt
     4321 cctggtgccc tgcccgctcg gctggcgctt caagtcggag tcgacgatcg aggtgtcgcg
     4381 cttggcggtg ctctcgaacc tgttcccgct ggtcgagtac cggggcggga tcctggacaa
     4441 ggtgaagcgg atcggcgccc ccgtccccgt ggacgactac ctccgggcgc aggaccgctt
     4501 caagcacctg ttccgggacg agcgcgggga ggtggagcgc gagttcctgc agtcgatcgc
     4561 cgacgagaac gcgcgccgcc tggggctccc cgtctaccgg cccgcggcct gacccgctcc
     4621 cccgacggag ggtccccggc ggaaccacga ggccggctgg ggcgtctcag tcgcgaacgg
     4681 cggggttacg aacgtccgat tcgggggaag acgaatgccg aaccgtcccg aaggggggag
     4741 tgatatggcg ctcagggccg acgatctgcg gcgggacgtt ctctaccggc gttacctgca
     4801 ggctctcgcg aggttcggcc agccggagga accggcgccg gccccgggcc acgcgatccg
     4861 gagctgcccc acctgcggcc ggcacaccat gttccgtctc gaccccgacg ggacgtggtt
     4921 cgagtgcacc cgctgcggcc actacgcctg accggcccgt tcacctgacc ggctaggccc
     4981 agccctcgaa cagctcgttc agctcctcgg tcgtgcccgt cgggtagagc tgctcgaacg
     5041 tgcacgactc cggcggcttg tcggggcgcc agcggatgaa gcgggcgccg tggcggatcc
     5101 ggtgcccggt cacctggtcg aaccagacct ccacgactag caccgggtcg agcggctggt
     5161 actccgcctc ctcctcgctc cgccagcggc tcgggcctcc cgggccggcg ccgctgaacc
     5221 cggccccgcc catgtgcggc ttcaggacct tcgccacctc cacacgatcc ttcgccgaga
     5281 agccgctcgt gaacccgacg ttgtgcagct tcccctcgtg gaagagcccg agcaggaggg
     5341 agccgatggc cttgccgtgg gcgttcttgt accagcggaa cccaccgacg acgcagtcgg
     5401 ccgtgcgctg gcgcttgacc ttgatcatct cgcgctcgcc gctcttgtaa gggcggtcgc
     5461 cgcgcttcgc gaccacgccg tccagcccgg ccacgtcgta ctcggtcagc cagccacgcg
     5521 cctcgtcggg gtccgcggtc tggggcgaca ggagcaatgg cgtcttcgcg cgcttcatcg
     5581 cccgctccag ccgcgcccgg cgctcggcga accccacgcc gcgcaggtcc tccgtgcctt
     5641 cggcgagcag gtcgaacgcc acgaacgacg acggcgtcgt ctccgaaagc atcctcactc
     5701 gggacgccgc cggatggatc cgctgcaaca gcgcgttgaa gtcgagcccg cgcttcccga
     5761 agatgacgat ctcgccgtcg tagacgccgt ccggaatctt caccgccgcg aacgccgcct
     5821 ccagctccgg gaagtaccgg ttgaacggca actgcttgcg cgaggtgatc gtcacgttcg
     5881 gcccgtcccg ccagacgatc gagcggaacc cgtcccactt cggctcgtac cgccagtccg
     5941 agccggtcgg gacgtcggcc gcgaccttcg cctccatcgg cgcgacggtc ggcttgaacg
     6001 ggagcgtcac gctcgtgagt ctcccacggc gagcgcgatc ggggggctcg gcatggcggc
     6061 ctcccttcta cgcttgttgt cgaggggagt cactcgccgc tgtggtcgtc tcacgttcca
     6121 aagcatcgag caggtccctc ccggcgcgag ccggaacggc cccccggccg gacagcacag
     6181 ggcgccgggg agccaggaaa ggagggatcg gcttcatggc caccggcaag gtcaagcggt
     6241 tcagccccga gaaggggtag gggttcatca cccgcgacga cggccccgac gtgttcgtcc
     6301 acttctccgc catcgagggc gcgggctacc gcaacctcga ggaggggcag acggtggaat
     6361 tcgaggtcac gcagggcgcc aagggcgacc aggcctccaa cgtccgcatc gtcggcgcct
     6421 gaccaccacc acgagcggcg agcgtccgtc cgcggcccgg ctacggggtg tagggcgggc
     6481 gctcctcgcc tccggctcgg ccggcccccg ggcctctcgg gacgcgcggc cgtggcgctc
     6541 atcaggcggc ggccgcccgg gttcgcgccc gccccaccag gtagccggtc acgtaggccg
     6601 ccactcccgt cgcggcggcg ctcgcgcccg cgacggaggc caccagcgcg cggcgccgtc
     6661 ccgcgcggaa gtcgagcacc ggccagccgc gccgccgggc ctcctgcagg aggtggcggt
     6721 ccgggttcgt ggccaccggg ttgcccacga cctcgaacag cgggacgtcg ttcacgctgt
     6781 cggagtaggc gtacgagccc tcgagatcga ggtgctgctc gtcggcgacg tcgagaacgc
     6841 gccgggcctt ctcctccccg tggcatggct cgccgtccag ctcccccgtg tagcggccat
     6901 cgtcgtccac gagggcccgg gtgccgagca cgccgtccag cccgaggtcc gcggcgaaca
     6961 gctgcagcag gtcctccggc gcggccgagc agacgtagac ctgccgaccg tggcgctggt
     7021 gccgcgcgac cagctcgagc gcctgcgggt acatccgagg cagcagctcc aggcggacca
     7081 ggtcccggcc gagctcgagc acctcctcgc ggctgcgctc gcggatcccg tcgagcgccc
     7141 cctggcgggc ccgtcggatg cgccccgggt cctcggtggc gaacaggcgg tacgcggcct
     7201 ggtcgagcgt gaagcgggcg atgtccccgg tggtgaagta cccgcggcgg aacatctctc
     7261 gcgcgatcgg gaaaaggctc gatccgggga tcagcgtgcg atcgagatcg aagaacgcgg
     7321 cctcggtcat gaccgagcag tctagccggg ccttccgacg accccgtcta ctagtctctc
     7381 ctccatgccg gaacgggatc cgaaccacgt gcccaccgcc cacctgacgc gaaccgccct
     7441 cgagcggctg caggccgagc tgcacgagct gaagacctcc ggccgcgagc gcatggccga
     7501 gcgcctgcag cacgcccgcg agatgggaga catccgcgag aacgccgagt acgacgcggc
     7561 gaagaacgag cagggcctca tggaggcccg catccgcacg atcgagcgcc tgctgaagga
     7621 cccggtgatc gtcgagaagg gcgagcactc ggacgaggtg gcggccggca tggtggtcac
     7681 ggtgctcccc ctggacgacg gtgacggcgc cgacgaggag gagtacctgc tggcggcgtc
     7741 ggcggaggag cggcacccgg gcgcgcggac ggtgaccacc gagtcgccgt tcggcaaggc
     7801 gctgctcggc tcgcgcaccg gcgatactgt tcgctacgct gcgcccggcg gcgagttctc
     7861 ctaccgggtc atccgcttcg agccccacgg ctgaggagcc cacacggacg cggtccgcga
     7921 tgccggacct tcccccaggc tggtgcgcac ggcgcttccg gtcgacctcc cgctgagcct
     7981 cggcccgctg gcgtgcggcc ggtgggaccc gacgatccgg gccgatggcg gtgcgtggtg
     8041 gcgcgccacc cgcacggcga ccggcccggc caccgtccgc tacgcgccgg cggacggtgg
     8101 cgtgcgggtg cgggcctggg ggcccggcgc cgaggcggcg ctcgcgctgg cgcccgacct
     8161 gctcggcgca cgcgacgaac tcgatggctt cgaccctggc gccggcctgg tccacgagct
     8221 gcatcggcgg ttctccggtc tgcgggtcat ccgctcgcgg gccgtgttcc aggcgatcgt
     8281 ccccgccgtg ctcgagcagc aggtcaccgg gaaggagtca cgggcgtcct ggcggcggct
     8341 ggtgctccgg tacggggagc cggcccccgg gccgctcgcc gggctgatgc tgccgccggc
     8401 ggcggcgacg ctcgcggcgc ttccgtacca cgcgttccac ccgctcggcg tcgagatgcg
     8461 ccgggcgaac acggtccgcg gcgtggcggc cggcgccgcc cgcctggagg cgaccgccgc
     8521 catgccgctc gccgacgccc accgccggct gcgcgcgttc cccggcgtcg ggccgtggac
     8581 ggcggcggtg gtggccgggg tggcgctcgg cgacggcgac gcggtgcccg tcggcgatta
     8641 ccacgtcaag cacatggtct cctgggcgct cgccggcgag ccacgcggca ccgacgagcg
     8701 gatgctcgag ctgctcgagc cgttcgccgg ccaccgcggc cgcgtggtcc gcctgatcaa
     8761 ggccgccggc ctcgccgccc ccaggttcgg cccgcggatg ccggcgcgct cctacgagcg
     8821 gctctaggga tccgccgcga gcggctggca ggaagggcag aggaacgtca gacgctcggc
     8881 cgccggcggg ccctgggcga tgcgctcgat cggggtcccg cagcggcggc acggctcgtc
     8941 ggcacggcgg tagacccagc gcccatggcc gcggcgggcg atgccggtcg
//
LOCUS       DASPWO010000514         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_839844, whole genome shotgun sequence.
ACCESSION   DASPWO010000514 DASPWO010000000
VERSION     DASPWO010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839844"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VEO00_11835"
     CDS             <1..213
                     /locus_tag="VEO00_11835"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HYU58723.1"
                     /translation="EALALAATITANGPVAVRKSLRMLREAGDLGEADAWQRSNEIAL
                     EVFGTNDAIEGATAFAEKRPPKWTGT"
     gene            258..1037
                     /locus_tag="VEO00_11840"
     CDS             258..1037
                     /locus_tag="VEO00_11840"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYU58724.1"
                     /translation="MGSGERMRVEDLARRAEVSVDTIRFYQKRQLLPPPEREGRIAWY
                     QPDHIERLGRIRDLQRRGFSLAVIRRLLAGELDATDEPLAAAVAAAEGAGPTEFLTLE
                     ELAERAGVPLALIESVAKEGLLIPRLHEGQARYTAGDADVVAAGLRLLEAGLPMPELL
                     ALARHHHEATRVTAEEAVAMFDSHVRKPLRDSDLPAEERAERLVDAFRVLLPSVTALV
                     AHHFRRILLQVAQEHLEQVGEPAELAAAEEEARRTLEAVWT"
     gene            1034..1738
                     /locus_tag="VEO00_11845"
     CDS             1034..1738
                     /locus_tag="VEO00_11845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HYU58725.1"
                     /translation="MSRTTRPAPLPEGTEKRREVESMFDRIAPRYDLVNRVMTLGLDR
                     RWRRRTITALGLPSGARVLDLGCGTGDLCEELRARAHAPVGIDLSAGMLGAAHTIAPL
                     VRGDALRLPVPEGTIDGVVSGFALRNVVDVGALFSECARVLRVGGRIAMLETDEPTNP
                     VLHAGHRLWFRRIVPALGGRLGRDREAYRYLPRSAAYLPPRHELLELIGTAGFDAVRH
                     QELGGGAVQLLTGTRR"
     gene            1735..>2035
                     /locus_tag="VEO00_11850"
     CDS             1735..>2035
                     /locus_tag="VEO00_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58726.1"
                     /translation="MTRAAPAAATLFARTREIDPPADLLDALGDNGFAWLDGTGGLVT
                     AGVAARLRPAEAGESLASVTLDDPLARPGTGAIAVGALPFAGAGELVVPHTVTAVA"
BASE COUNT          273 a          767 c          732 g          263 t
ORIGIN      
        1 gaagccctcg cgctcgcggc caccatcacc gccaacggcc cggtggcggt gcgcaagtcg
       61 ctgcggatgc tgcgcgaggc cggcgacctc ggcgaagccg atgcgtggca acgcagcaac
      121 gagatcgcgc tcgaggtgtt cgggacaaac gacgccatcg agggcgcgac cgcgttcgcc
      181 gagaagcggc cgcccaaatg gaccggcacc tgagccggat gcgaccgcgg gagtaactgc
      241 tcctagaatc ggctttcgtg ggatcgggag agcggatgcg ggtcgaggac ctggcccggc
      301 gggccgaggt gtccgtcgac accatccgct tctaccagaa gcgtcagctg ctcccgccgc
      361 ccgagcgtga gggccgcatc gcctggtacc agcccgacca catcgagcgg ctcggccgca
      421 tccgcgatct gcagcgccgc gggttctcgc tcgcggtcat ccgccggctc ctcgccggcg
      481 agctcgacgc caccgacgag ccgctggcgg cggcggtggc ggcggccgaa ggcgcggggc
      541 cgaccgagtt cctcacgctg gaggagctcg ccgagcgcgc cggcgtgccg ctggcgctca
      601 tcgagtcggt cgcgaaggaa ggtctcctca tcccgcggct gcacgagggc caggcgcgct
      661 acaccgccgg ggacgccgac gtcgtcgccg ccggcctccg gctcctcgag gctggcctac
      721 ccatgcccga gctgctcgcg ctggcacgcc atcaccacga agccacccgc gtgaccgccg
      781 aggaagcggt ggcgatgttc gacagccatg tccgcaagcc cctgcgcgac tcggacctgc
      841 cggcggagga gcgggccgaa cggctggtcg acgcgttccg ggtgctcctg ccctcggtga
      901 ccgcgctggt cgcgcaccac ttccggcgga tcctgctcca ggtcgcgcaa gagcacctcg
      961 agcaggtcgg cgaaccggcg gagttggcgg cggccgagga agaggcacgc cggacactcg
     1021 aggcagtgtg gacgtgagcc gcacgactcg ccccgcgccg ctgccggaag ggactgaaaa
     1081 gcgacgcgag gtcgagagca tgttcgaccg catcgcaccc cgctacgacc tcgtcaaccg
     1141 ggtcatgacc ctcgggctcg accggcggtg gcgacgtcgc acgatcaccg cgctgggact
     1201 tccgagcggc gcgcgggtac tcgacctcgg ctgcggcacc ggcgacctgt gtgaggagct
     1261 ccgcgcccgc gctcacgcgc cggtggggat cgatctctcc gccggcatgc tcggagccgc
     1321 gcacacgatc gcgcccctcg tgcggggcga cgccctgcgg ctgccggtac cggagggcac
     1381 aatcgacggc gtcgtctccg gcttcgcctt gcgcaacgtc gtcgacgtgg gcgcgctgtt
     1441 ctccgaatgc gcgcgcgtgc tgcgcgtcgg cggccgcatc gcgatgctcg agaccgacga
     1501 gcccacgaac ccggtgctgc acgccgggca ccggctctgg ttccgacgca tcgtccccgc
     1561 gctcggcgga cgcctgggac gggaccgcga ggcctaccgc tacttgcccc gctcggccgc
     1621 gtaccttccg ccccggcacg agctgctcga gctgatcggc accgccgggt tcgacgccgt
     1681 gcgccaccag gagctcggcg gcggcgcggt gcaactcctg accgggaccc ggcgatgacc
     1741 cgcgccgcgc cggccgcggc cacactgttc gcccgcaccc gggagatcga cccaccggcc
     1801 gacctcctcg acgcgctggg cgacaacggg ttcgcctggc tcgacgggac cggcgggctc
     1861 gtcaccgctg gggtggcggc tcggctccgg cccgccgagg ccggcgagtc gttggcttcg
     1921 gtcaccctcg atgatccgct ggcgcgcccg ggcaccggcg cgatcgcggt gggtgcgctc
     1981 cccttcgcgg gcgccggcga gctcgtcgtc ccccacacgg tcaccgcggt ggccg
//
LOCUS       DASPWO010000515         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_583985, whole genome shotgun sequence.
ACCESSION   DASPWO010000515 DASPWO010000000
VERSION     DASPWO010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583985"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VEO00_11855"
     CDS             complement(<1..798)
                     /locus_tag="VEO00_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58727.1"
                     /translation="MRARAAPLSRAARARALGAAIAWPGVIVAAVALVLHDFAFGLHV
                     RGGDLLDFFAPNHCFLGTSLRAGHIPAWNPYALAGVPFAADPQAGWLHLPAMFLYTAL
                     PCGAAAPMLVVLQPALAGVGLYAFLRGERVSRPSAALGGLALGVGTAGSTLVLSLPFA
                     GTLAWTPWLLAAAGRALRAVRWSPRLAWTAAAAVAWGQLAAAHASDGLFVGTAALLAY
                     AGATMWTAVGRDGRPARSFLGPAGVLLAALPLVNLAILVPRAAYLPRT"
     gene            complement(774..>2210)
                     /locus_tag="VEO00_11860"
     CDS             complement(774..>2210)
                     /locus_tag="VEO00_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58728.1"
                     /translation="LNPETTEDLAQYKAETFGRLVAVCALLLAVRAVRGGTWGGSSPP
                     GAPERTSSSERTPRTRRSSRGETVVAGALFGVGAGTHLVPVIAALAFLVFYALAVLVI
                     ERDARGLARTAGVMGAVAVVVAAAALVLPKGDIGFGGAAGGDEAYARFGTDFDPTRFF
                     LSGNLELAQRPPPPGWYYDGGFLFDQLIESGTGRPFSRGAGLAFAAGGLVLAGVMLWR
                     FPDDLRPLGVAAWGLGLTLFLVAVWFSARSDLYILARFGLRRMYDYASVPFALLGLAL
                     LEVALAALTRVEATVPAVAGAAVVLAGTIWIAPGGRADPARIAEGLRGVAPLEWIRDH
                     APCDARILSNQRTAGSFQALTGRVGVLEGMAPYLRPEILVDVVDLHLAAREFFADPAR
                     RALTLVLQDVDYVIVTRGQIIGAAGAEFRAAPALDEVPFLTLAFRSPLARIYRVSHDE
                     LPGLDLPVPPPPDPVDFPGYACARGPLR"
BASE COUNT          347 a          862 c          803 g          198 t
ORIGIN      
        1 cgtccggggc aggtaggccg cgcgcggcac caggatcgcc aggttgacga ggggcagcgc
       61 ggcgagcagc accccggccg gcccgaggaa cgaccgggcc ggcctgccgt cccggccaac
      121 cgccgtccac atcgtcgccc cggcgtaggc gagcagcgcc gccgtcccga cgaacagacc
      181 gtcgctggcg tgcgctgcgg cgagctgccc ccaggccacc gccgcggccg ccgtccaggc
      241 gaggcggggg ctccagcgca ccgcccgaag cgcccttccc gccgcggcta ggagccacgg
      301 cgtccaggcg agcgttccgg cgaacggaag cgacagcacg agggtcgacc cggccgtccc
      361 cacgccgagc gccagccctc cgagcgccgc cgacggccgc gaaacccgct cgccgcgcag
      421 gaaggcgtac aggccgaccc cggcgagcgc cggctgcagc acgacgagca tcggcgcggc
      481 ggcaccgcag ggaagggccg tgtagaggaa catcgccggc aggtgcaacc accccgcctg
      541 agggtccgcc gcgaacggca ccccggcaag cgcgtacggg ttccaggccg ggatgtgccc
      601 ggcccgcagg ctcgttccca ggaagcagtg gttcggcgcg aagaagtcga ggaggtcgcc
      661 gccgcgaacg tgcagcccga acgcgaagtc gtgcagcacg agcgcgacgg ccgcgacgat
      721 cacccccggc caggcgatcg cggcgccgag cgcgcgcgcc cgcgccgcgc ggctcagcgg
      781 agcggcccgc gcgcgcacgc gtagcccggg aagtcgactg ggtcgggagg aggcggcacg
      841 gggaggtcca gccccggcag ctcgtcgtgc gatacgcggt agatccgcgc gagcggcgaa
      901 cggaaggcga gggtgaggaa tggcacctcg tccagcgccg gcgccgcgcg gaactcggcc
      961 ccggctgcgc cgatgatctg gcctcgggtc acgatcacgt agtcgacgtc ctgcaggacg
     1021 agggtgagcg cgcgtcgcgc gggatcggcg aagaactccc gggccgccag gtgcaggtcc
     1081 accacgtcga ccaggatctc cgggcgcagg tacggcgcca tcccctcgag cacccccacc
     1141 cggccggtga gcgcctggaa cgatccggcg gtgcgctggt tcgacaggat tcgggcgtca
     1201 cacggggcgt ggtcacggat ccactcgagc ggcgcgacgc cgcgcagccc ctccgcgatc
     1261 cgcgccgggt cggcccgccc gcccggcgcg atccagatcg tcccggccag caccacggcc
     1321 gcgccggcca cggccggcac cgtcgcttcg acgcgcgtca gcgcagcgag ggcgacctcg
     1381 agcagcgcca gcccgagcag cgcgaacggg accgacgcgt agtcgtacat ccggcgcagg
     1441 ccgaaccggg cgaggatgta gaggtccgag cgcgccgaga accagacggc caccaggaag
     1501 agcgtcaggc cgagccccca ggccgccacc ccgagcggcc gcagatcgtc cgggaagcgc
     1561 cagagcatca cccctgccag gacgagccca ccggcggcga aggccagccc cgccccgcgc
     1621 gagaacggcc gcccggttcc cgactcgatc agctggtcga agaggaaccc accgtcgtag
     1681 taccagccgg gcggcggcgg tcgctgcgcc agctcgaggt tccccgacag gaagaagcgg
     1741 gtcgggtcga agtccgtgcc gaagcgcgcg tacgcttcgt cgccgccggc cgcccccccg
     1801 aagccgatgt cccccttcgg caggacgagg gccgcggcgg ccaccacgac ggccacggct
     1861 cccatcaccc ccgccgtgcg ggccagcccg cgagcgtccc gctcgatgac caggacggcc
     1921 agcgcgtaga acacgaggaa cgccagcgcc gcgatcaccg gcaccaggtg cgtccccgcg
     1981 ccgacgccga agagtgcgcc ggcgaccacc gtctccccgc gagatgagcg tctcgtccgg
     2041 ggggtccgct cgctcgacga ggtccgttcc ggcgctcccg ggggcgagct ccccccccac
     2101 gtccccccac gaacggcgcg gaccgcgagc agcagcgcgc acaccgccac caggcggccg
     2161 aacgtctcgg ccttgtactg cgccaggtcc tcggtcgtct ccgggttcag
//
LOCUS       DASPWO010000516         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1093673, whole genome shotgun sequence.
ACCESSION   DASPWO010000516 DASPWO010000000
VERSION     DASPWO010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093673"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1221)
                     /locus_tag="VEO00_11865"
     CDS             complement(199..1221)
                     /locus_tag="VEO00_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYU58729.1"
                     /translation="MQALVRCATLSGYIDLARVLGLETDRLLHDVGLNAADLAVPEKW
                     IPAGAVARLLAASAVESGHDDFALKLAERRRLSTLGPLSVVLREEPDLREALALLIRY
                     EHSYNESLRMTLTETDSLATVWLWFEFGEPAPTEQALALGLAALHGIIRACVDPDWRP
                     LSVCLTQHPPVHRETYRQLFGPGLRFDHEFTGLVMYAADLDRPNRLADPLMRPYAQQF
                     LDSVVAPRAATWTDRTRDLIELLLPLGKCSLDQAAKALGVDPRTLQRMLAGEGGSFST
                     LLHSVRAGLAERHLAGRRYSLTEVSEVLGFAAPSAFSRWFSQQFGVSPSAWRANTSAP
                     QREASA"
     gene            1366..2352
                     /locus_tag="VEO00_11870"
     CDS             1366..2352
                     /locus_tag="VEO00_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005257691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HYU58730.1"
                     /translation="MAKGFRFYQAGGPEVLQWEILEVGEPGPGEVRIRHAAVGLNFAD
                     SYFRSGLYPAPLPAGMGVEAAGVIEAVGPGVAGFSEGDRVTYTGSPLGAYSTERVMPA
                     EHLIKLPETIAFDTAAAMTMRGLTSAYLLRRIASLEEGDTVLLHAAAGGVGLIFTQWA
                     ALLGIEVIGTVSTDEKAEIAGARGCAHTIVYTRENVAARVREITEGAGVPVVYDSIGK
                     TTYAQSLDSLARRGLLVCFGTASGQVPPIDAMELAVKGSLFVTRPALADYIADADERA
                     ALAGELFDHVSSGAIRVEINQRYALEDAVQAHRDLESGRSTGSSVFDLDATL"
BASE COUNT          371 a          797 c          853 g          358 t
ORIGIN      
        1 gccggccgag cccgccgggc atcgagccga cccacaggcc gtgctcgtcc tctccgagcc
       61 ggaacgactg gatctgccgg tgggggcggc cgtcgtactt gcagaacagc gtcctcacaa
      121 cctccacgat ctgaccgtac gtgatcacgc gatcacttgc tgacgtaccc ggcgttttgg
      181 gtcggcccgg cgttttggct aggccgaggc ctcgcgctgc ggcgcggacg tgttggctcg
      241 ccacgcgctc ggactcaccc cgaactgctg actgaaccag cgcgagaacg cgctcggggc
      301 ggcgaacccg agcacctcgg agacctcggt cagggagtac ctccggccgg ccaggtggcg
      361 ttccgccagg ccggctcgga cggagtgcag gagtgtgctg aaggagccgc cctcacccgc
      421 gagcatgcgt tgcagggttc gaggatcgac gccgagtgcc ttggcggcct ggtcgaggga
      481 gcacttcccg agggggagca gcagttcgat gagatcgcgg gtgcgatccg tccaggtcgc
      541 cgcccgtggg gcgaccacgg agtcgaggaa ctgctgggcg tacgggcgca tcagggggtc
      601 ggcgagtcgg ttcgggcggt cgaggtccgc ggcgtacatc acgaggccgg tgaactcgtg
      661 atcgaaccgt aggcccgggc cgaacagctg gcggtaggtc tcccggtgaa ccggcgggtg
      721 ctgggtgagg cacaccgaga ggggccgcca gtccgggtcg acgcaggccc ggatgatgcc
      781 gtggagagcg gcgagtccga gtgccagtgc ctgctcggtg ggcgccggct cgccgaattc
      841 gaaccagagc cacaccgtgg ccagactgtc ggtctcggtc agagtcatcc gcagcgactc
      901 gttgtagctg tgctcgtagc ggatcagcag cgccagcgcc tcgcgcaggt ccggttcctc
      961 tcggagtacg acgctcaagg ggccgagcgt ggagagccgg cgccgttcgg cgagcttgag
     1021 cgcgaagtcg tcgtggccgg actccacggc cgaggccgcc aggaggcggg cgacggctcc
     1081 tgcggggatc cacttctccg gcacggccag gtcagcggca ttcaggccga catcgtggag
     1141 caggcggtcg gtctccagac ccagcacccg ggccaagtcg atgtacccgc tcagagtcgc
     1201 gcagcggacc agcgcctgca tcagagcccc cttcctgtcg cgatcaggca aggagattgt
     1261 cgtgatcagt aaagcgagcc gttcggggtc cacctagcgt aatgcgcatc acacagaccc
     1321 gatggcatgg cggtgcgggc tgtcccgaga aacaggagaa gctcgatggc gaagggtttt
     1381 cgtttctacc aggctggtgg gcccgaggtc ctgcagtggg agatcctcga ggtcggcgag
     1441 cccggccctg gcgaggtcag gatccggcac gcggcggtcg gtttgaactt cgccgacagc
     1501 tacttccgca gcggcctgta ccccgccccg ctgccggccg ggatgggggt cgaggccgcc
     1561 ggcgtgatcg aggccgtggg ccccggcgtc gccgggttca gcgaaggcga ccgggtgacc
     1621 tatacgggca gcccgctggg cgcgtacagc acggagcggg tgatgccggc cgagcacctg
     1681 atcaagctgc ccgagaccat cgcgttcgac accgcggcgg ccatgacgat gcgcggcctc
     1741 acctcggcgt acctcttgcg ccggatcgcg tcgctcgagg agggggacac ggtcctgctc
     1801 cacgccgcgg cgggcggtgt cggcctgatc ttcacccagt gggccgccct gctggggatc
     1861 gaggtgatcg gcaccgtgtc gaccgacgag aaggccgaga tcgccggtgc ccgcggttgt
     1921 gcgcacacca tcgtctacac ccgcgagaac gtggccgcca gggttcggga gatcacagag
     1981 ggtgccgggg tgccggtcgt ctacgacagc atcggcaaga cgacgtacgc gcagtccctc
     2041 gactcactcg cccgccgtgg cctgctcgtc tgcttcggca cggcgtcagg ccaggtgccg
     2101 cccatcgacg ccatggagct cgcggtcaag ggttcgctct tcgtgacccg gcctgcgttg
     2161 gccgactaca tcgccgacgc ggacgagcgg gcggctctcg ccggcgagct cttcgaccac
     2221 gtcagctccg gcgcgatccg cgtcgagatc aaccagcgct acgccctcga ggacgcggtc
     2281 caggcacatc gggacctgga gtccggccgc agcaccggct cctccgtttt cgacctcgac
     2341 gcgactctct agcccagcca cacaaccccc aggaaccag
//
LOCUS       DASPWO010000517         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_752783, whole genome shotgun sequence.
ACCESSION   DASPWO010000517 DASPWO010000000
VERSION     DASPWO010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752783"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VEO00_11875"
     CDS             complement(<1..302)
                     /locus_tag="VEO00_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58731.1"
                     /translation="MTAQTAIPAPPIDLGPVLPEVILAGVAMLLLVGESIWKRPPRWV
                     GPAASLAAVAGAAAASLWVWTLPVVTSGAAGSAGTELAGNAAAQPFTVLSGMVAADR"
     gene            complement(299..1819)
                     /locus_tag="VEO00_11880"
     CDS             complement(299..1819)
                     /locus_tag="VEO00_11880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HYU58732.1"
                     /translation="MFPWLTVVVLAPLVGAAVVAALPRGRDGAARWVALVTTVGTFVV
                     AVGIVGAYDGSRSGFQLVERAVWVRSLNFQYVVGVDGISLFLVVLTAFLMPLSILASW
                     TVGTRVKAYMVAMLVLETAMIGSFVALDLLLFFLFFEALLFPMYLIIGGWGSERRVYA
                     AVKFFLYTMAGSAFLFVAILFLYFRAGSEVGRATFDFTQLVGLPLSVAAGRWLFLAFF
                     VAFAVKVPLFPLHTWLPDAHTEAPTAGSVLLAGVLLKVGAYGLLRFNLSLFPEASEYF
                     RVFVSTLAVVGIVYGAVVAMVQRDVKRLVAYSSVSHLGFVVLGTFAFTLQGMSGGVLQ
                     MVNHGLSTGALFLLVGMLYERSHTRDLDALGGMGKTVPLLSGFFLLVALSSLGLPGLN
                     GFVGEFLILLGSFARHRALTVVGATGVILSAIYLLWAYQRMAHGEPRGDAVRFPDLSL
                     RELVILVPMVAAIVFIGVYPKPLLERIEPSVRDAGAAVMVRDAGPAVAEAGGQRTP"
     gene            complement(1823..>2965)
                     /locus_tag="VEO00_11885"
     CDS             complement(1823..>2965)
                     /locus_tag="VEO00_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HYU58733.1"
                     /translation="TPVSALIHAATMVTAGVYLVVRTHVLFEASDVALPIVAVVGTIT
                     ALWAATSALGQDDLKRVLAYSTISQLGFMFLAAGVGAYAVAIFHLVAHAFFKAVLFLG
                     AGSVMHALDGEIDLKRLGGLRRRMPVTTASFVAGALALAGIPPLSGFFSKDQILAGAY
                     GAGRFGLWIVALVAASFTALYVARATLLAFFGGPRHDRQPHESPVLMTAPMVVLAALA
                     VAGGALGLSAGGGAIVRFLGEGFAAGETAGGGLSELTLALIATGLSVVMLALGWLVYG
                     SGRFDWAALRVRLGGLHDTLAAGWYLDAAYGATIVAPGKLGAAFAAYVFDRGVIDGAV
                     NGVGRAVAAASRAGRRIQTGFVRSYALATFLGATALLLYLAFRFGG"
BASE COUNT          549 a         1100 c         1029 g          287 t
ORIGIN      
        1 cggtcggctg cgaccatccc cgagagcacc gtgaacggct gcgcggcggc gttcccggcg
       61 agctcggtcc cggcgctccc ggccgcgccg gacgtgacga ccggcagcgt ccacacccag
      121 agggaggccg ccgccgcccc ggccaccgcg gcgagggagg cggccgggcc gacccagcgc
      181 ggcgggcgct tccagatcga ctcgccgacc agcagcagca tcgccacgcc ggcgaggatc
      241 acctccggca gcaccggccc gaggtcgatc ggcggcgccg ggatcgccgt ctgcgcggtc
      301 acggcgttct ctgccccccg gcctccgcca ccgccggacc cgcgtcccga accatgacgg
      361 cggcgcccgc gtcccgcacc gagggctcga tccgctcgag gagcggcttc gggtagacgc
      421 cgatgaacac gatcgccgcg accatcggca ccaggattac cagctcgcgt agagagaggt
      481 ccgggaagcg gaccgcatct ccccgcggct caccgtgcgc catccgctgg tacgcccaca
      541 gcaggtagat cgcggacagg atcacgccgg tcgcgccgac gacggtcagc gcccggtggc
      601 gcgcgaacga gccgagcagg atcaggaact ccccgacgaa cccgttcagg ccgggcaggc
      661 cgagcgagga cagagcgacg aggaggaaga atccggagag cagcgggacc gtcttgccca
      721 tgccgccgag cgcgtcgagg tcacgagtgt gcgaccgctc gtagagcatc ccgaccagca
      781 ggaagagcgc cccggtggag agcccgtggt tcaccatctg cagcaccccg cccgacatcc
      841 cctgcagcgt gaacgcgaag gttccgagca cgacgaaccc gaggtgcgag accgacgagt
      901 aggcgaccag gcgcttcacg tcgcgctgga ccatcgccac gacggcgccg tagacgatcc
      961 cgacgaccgc gagcgtcgag acgaacaccc tgaagtactc cgaggcttcg gggaagaggg
     1021 aaaggttgaa gcgcagcagc ccgtaggcgc cgaccttcag caggacgccg gccagcagga
     1081 ccgatccggc cgtcggcgcc tcggtgtgcg cgtcgggcag ccacgtgtgc agcgggaaca
     1141 gcggcacctt cacggcgaac gccacgaaga aggccaggaa cagccagcgg ccggccgcca
     1201 ccgacagcgg gaggcccacc agttgcgtga agtcgaacgt ggctcgcccc acctcggagc
     1261 ccgcccggaa gtacaggaac aggatcgcca cgaacaggaa ggccgacccc gccatggtgt
     1321 acaggaagaa cttcaccgcc gcgtagacgc ggcgctcact cccccacccc ccgatgatca
     1381 ggtacatcgg gaacagcagc gcctcgaaga acaggaagaa gagcaggagg tcgagcgcga
     1441 cgaacgatcc gatcattgcg gtctcgagga cgagcatcgc caccatgtag gccttcaccc
     1501 gcgtcccgac ggtccacgag gcgaggatcg agaggggcat caggaacgcg gtcagcacca
     1561 cgaggaacag gctgatgccg tccacgccga cgacgtactg gaagttgagc gatcggaccc
     1621 agaccgcgcg ctcgacgagc tggaacccgg accggctccc gtcgtaggcg ccgacgatcc
     1681 cgacggcgac gacgaacgtc ccgaccgtcg tgaccagcgc cacccaccgc gccgcgccgt
     1741 cccggccgcg gggcagcgcg gccacgacgg cggctcccac cagcggcgcg agcaccacga
     1801 ccgtcagcca cgggaacacc ggtcagcctc cgaagcggaa tgccaggtag aggaggagcg
     1861 ccgttgcccc caggaacgtg gccagggcgt aggagcggac gaatccggtc tggatccgcc
     1921 ggccagcccg cgacgccgcc gcgacggccc gcccaacccc gttgacggcg ccgtcgatga
     1981 cgccccggtc gaagacgtag gccgcgaagg cggcgccgag cttgccgggg gcgacgatcg
     2041 tcgccccgta tgccgcgtcg aggtaccagc cggcggcgag cgtgtcgtgc agacccccga
     2101 gccgcacccg cagcgccgcc cagtcgaacc ggcccgaccc gtacaccagc cagcccagcg
     2161 cgagcatgac caccgacaga ccggtggcga tgagggccag ggtcagctcc gagagccccc
     2221 cgccggcggt ctcccccgcg gcgaacccct cgccgaggaa ccggacgatc gcgccgccgc
     2281 cggccgacag gccgagcgct cccccggcca ccgcgagcgc ggcgaggacg accatcggcg
     2341 cggtcatcag caccggcgac tcgtgcggct gccggtcgtg ccgcgggccg ccgaagaacg
     2401 ccagcagcgt cgctcgcgcc acgtagagcg cggtgaagga ggccgcgacg agcgccacga
     2461 tccacagccc gaaccggccg gccccgtagg ccccggccag gatctggtcc ttgctgaaga
     2521 acccggagag cggggggatg ccggcgagcg cgagcgcccc ggccacgaac gaggccgtgg
     2581 tcaccggcat ccgtcttcgc agacccccca gccgcttcag gtcgatctcg ccgtccaggg
     2641 cgtgcatcac gctccccgcg ccgaggaaca gcacggcctt gaagaacgcg tgggcgacca
     2701 ggtggaagat ggccacggcg taggcgccga cgccggccgc gaggaacatg aaccccagct
     2761 ggctgatcgt cgagtaggcg agcacgcgct tcaggtcgtc ctgcccgagg gccgacgttg
     2821 cggcccacag cgcggtgatc gtcccgacca cggcaacgat ggggagcgcc acgtccgacg
     2881 cctcgaagag cacgtgggtg cgcacgacga ggtacacacc ggccgtgacc atcgtcgcgg
     2941 cgtggatcag cgccgacacc ggcgt
//
LOCUS       DASPWO010000518         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_922514, whole genome shotgun sequence.
ACCESSION   DASPWO010000518 DASPWO010000000
VERSION     DASPWO010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922514"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1221)
                     /locus_tag="VEO00_11890"
     CDS             complement(238..1221)
                     /locus_tag="VEO00_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58734.1"
                     /translation="MRRSRLALAALLSFPAVFGGALVAPPAAARSPAVRLVAPQPGEA
                     RGRVIVRVDAEGDPAGAAFEVRPRGSGWDEALAVPLQPSAGGFEAVLDTRPLPNGAYR
                     LLARAWWADAVAYDPADGDTFAADGIALSVANPPPRPALASPRAGRGRVSLSWTQVAT
                     SDRSDFAGYGVYRAAARSGRCSAFGPLFVRVETTFGTTHVSSGLARGRYCFAVVALRT
                     SPVAAEVASRATAPSLVRVVRGGPPVSEGGDPDAAQGPYVPTLPYDPADLVKVVAAVI
                     ERGGLRDVGPLGSGRERQALVFVAVGLLLAVAAAGLLFAMRVPRETPARLR"
     gene            complement(1232..1996)
                     /locus_tag="VEO00_11895"
     CDS             complement(1232..1996)
                     /locus_tag="VEO00_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU58735.1"
                     /translation="MISSARRFPILEIDGLSAGYRRDAPAVREASLEVGEHEVVAVLG
                     SNGAGKTTLLRAVCGLVPHGAGRVRFLGEEVGGLRPHVLASRGLVLAPQARELAPSVS
                     VRDTLLLGAYPFRRDRGRVRRLLHDAYARFPILAERSRQRAGSLSGGERQQLMLARAL
                     MTRPRLLLLDEASAGLAPRLVRDLFTLIHALWEEGVTILLVEKAARAALEVADRAYVM
                     ELGVIVAEGPADEIAADPRLVRASLGPPPGERSGGR"
     gene            complement(1993..>2181)
                     /locus_tag="VEO00_11900"
     CDS             complement(1993..>2181)
                     /locus_tag="VEO00_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU58736.1"
                     /translation="VVVEHHLEFLRSLADQVTVLDAGRVIATGPPDLVRSDPAVVEAY
                     LGAPGGLGDAAASEMGHA"
BASE COUNT          312 a          857 c          811 g          201 t
ORIGIN      
        1 cctgaccacc tcgcccaacc gcctggggcg caagcggccg ggcactgccg gggccctcgc
       61 cggattacgc tacggcggcc acctcatcga catcggcgac ggatcggagc ggcgcgacgg
      121 tcacgctcgc cggcgcggaa cagtcgccgg gaaccgacta cgcgagcggg catgcggtcc
      181 ctcgcgcctg cccggtagct ccctcaaccc atctcactcc ggacgcgagc gtcggcgcta
      241 gcgaaggcgg gccggggtct cgcgcgggac gcgcatggcg aacaggagcc ccgcggccgc
      301 cacggcgagc agcagcccga cggcgacgaa gacgagcgcc tggcgttccc gacccgaccc
      361 gagcggcccg acgtcgcgca ggccgccgcg ctcgatcacc gcggccacca ccttgaccag
      421 gtcggcgggg tcgtacggga gggtcgggac gtacgggccc tgagctgcgt ccgggtcgcc
      481 gccctcggag accggggggc ctccgcgcac cactcgaacg agggatgggg cggtcgcccg
      541 cgaggccacc tccgccgcga ccggtgacgt ccgcagcgcc acgacggcga agcagtaccg
      601 gccgcgcgcc agcccgctcg agacgtgcgt cgtgccgaac gtcgtctcga cgcggacgaa
      661 gagagggccg aacgcggagc accggccgga ccgtgcggcg gcacggtaga ccccgtaccc
      721 ggcgaagtcg gaccggtcgg acgtggccac ctgcgtccag gagagcgaca cgcgcccccg
      781 gccggcccgg ggagaggcga gggccgggcg cggcggcggg ttcgccacgg agagcgcgat
      841 cccatcggcg gcgaacgtgt cgccgtcggc tgggtcgtac gcgacggcgt cggcccacca
      901 ggctcgtgcc agcagccggt acgccccgtt cggcagcggg cgggtgtcga gcacggcctc
      961 gaaccccccg gccgagggtt ggagcggcac cgccagcgcc tcgtcccagc cgctcccccg
     1021 cgggcgcacc tcgaaggcgg cgccggccgg atcgccctcc gcatccacgc gcacgatcac
     1081 gcggccgcgc gcctcgccgg gctggggggc gacgagccgc accgccgggg agcgcgccgc
     1141 cgcgggcggc gccacgaggg ccccgccgaa cacggccggg aacgagagca gcgctgcgag
     1201 cgcaaggcga ctccggcgca cggcccgcag tctaccggcc gccggagcgc tccccggggg
     1261 gcggaccgag ggacgccctc accaggcgcg ggtcggcggc gatctcgtcc gccgggcctt
     1321 cggccacgat cacccccagc tccatcacgt acgcccggtc ggcgacctcg agggcggccc
     1381 gggccgcctt ctcgaccagc aggatcgtga cgccctcctc ccacagcgca tggatcagcg
     1441 tgaacaggtc gcgcacgagc cgcggcgcga gcccggcgga cgcctcgtcc agcagaagga
     1501 gtcgcggccg cgtcatgagg gctcgggcga gcatcaactg ctggcgctcg ccgccggaca
     1561 gggacccggc ccgctgccgc gagcgctcgg cgaggatcgg gaagcgcgcg tacgcgtcgt
     1621 gcagcagccg gcgcacccgc ccccggtcgc gccggaacgg ataggcgccg agcaggagcg
     1681 tgtcgcgcac gctgacgctc ggcgcgagct cgcgagcctg cggcgcgagc accagccccc
     1741 gggaagccag cacgtgcggg cgcaggcccc cgacctcctc gccgaggaag cggaccctcc
     1801 ccgcgccgtg cggcacgagg ccgcacaccg cccgcaggag cgtggtcttg ccggcgccgt
     1861 tcgagccgag cacggccacg acctcgtgct ccccgacctc gagcgacgcc tcgcgcaccg
     1921 ccggcgcgtc gcgccggtac ccggcggaga gcccatcgat ctcaagaatc ggaaaccgcc
     1981 gggcggatga gatcatgcat gccccatctc gctggcggcg gcgtccccca ggccgcccgg
     2041 ggcgccgagg taggcctcga ccacggccgg gtctgagcgc acgagatccg gcggcccggt
     2101 ggcgatcacg cgcccggcgt cgagcaccgt cacctggtcg gcgagcgacc gcaggaactc
     2161 caggtggtgc tccaccacga c
//
LOCUS       DASPWO010000519         4088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_414248, whole genome shotgun sequence.
ACCESSION   DASPWO010000519 DASPWO010000000
VERSION     DASPWO010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414248"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VEO00_11905"
     CDS             <1..357
                     /locus_tag="VEO00_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan phosphorylase"
                     /protein_id="HYU58737.1"
                     /translation="ASGTSGMKAALNGGLNLSVLDGWWEEAYDGSNGWAIEGDVEADM
                     AAQDARDAATLYDLLEREVVPLFHDRDERGIPRGWMARVKASLRTIGPRYCAARMLDD
                     YVRDVYAAQPAGAAVR"
     gene            complement(391..2493)
                     /gene="glgX"
                     /locus_tag="VEO00_11910"
     CDS             complement(391..2493)
                     /gene="glgX"
                     /locus_tag="VEO00_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014912878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HYU58738.1"
                     /translation="MRVWPGEPYPLGATYDGLGVNFAIFSEHAEAVELCVYESPDDPA
                     ESARIELRERTDLIWHCYLPDARPGLAYGYRVRGPYAPDHGHRFNPHKLLIDPYAKAI
                     AGPIRWDDALLGYRPGSEESEPDERDSGPVMPKCMVIDPAFAWEGDRRPAVPWNRTII
                     YESHVRGMTMLHPEIPEELRGTYLGMAQDPVLDHLVSLGVTTVELMPVHQSVAEHELS
                     ARGLTNYWGYNSIAFLAPEVRYAAGRLGGQVDEFKSMVKAFHRAGIEVVLDVVYNHTG
                     EGNHLGPTLSLRGVDNAAYYRLDPEDRSRYLDFTGCGNSLNMLHPRTIQLIMDSLRYW
                     ITDVHVDGFRFDLAPALARELYEVNRLGTFFDIIQQDPTISQVKLIAEPWDVGPGGYQ
                     VGNFPIGWAEWNGKYRDCVRRFWRGDDGLVSELASRLSGSSDLYAAGGRHTYATINFV
                     TCHDGFTLHDLVSYERKHNEANGFDNTDGADENHSSNWGTEGETGNQRVVHLRERAKR
                     NFLATLLLSQGVPMLSHGDEIRRSQRGNNNGYCQDNDLSWVRWDLSTAERSLLEYTRL
                     LIRLRNENPVLRRRSFFTGRPIDARGTKDVTWLRRDGQEMSDEDWADEDRHVLGMLIH
                     GRATDEVDQRGRPVSGDTVLLLMNGGDRPRLFQLPAVGEGRWHEELNTARAGARVVRK
                     PGVNLAAHSLIFLRLAEH"
     gene            complement(2490..>4088)
                     /gene="glgB"
                     /locus_tag="VEO00_11915"
     CDS             complement(2490..>4088)
                     /gene="glgB"
                     /locus_tag="VEO00_11915"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HYU58739.1"
                     /translation="RVDKADPFALRAEVPPRTGSIVWHLDHEWGDGEWMRTRARRQSL
                     DSPMAIYEVHLGSWMRAPEDGDRWLTYRELAPRLSAYCAEQGFTHVEFLPITEHPFYG
                     SWGYQTTGYFAPTARYGKPQDLMDLIDELHRAGIGVILDWVPSHFPTDEHGLVFFDGS
                     HLYEHEDPKLGYHPDWNSAIFNYGRNEVRSFLMSSAMHWLDAYHADGLRVDAVASMLY
                     LDYSRGPGEWIPNEYGGNENLEAIGFLRRFNEDVYRAHPDVQTIAEESTAWPMVSRPT
                     HVGGLGFGLKWDMGWMNDTLGYAAREPVHRRYHHGNLTFRAMYAGSENFVLPLSHDEV
                     VHGKGSLLGKMPGDDWQRFANLRLLLAHLYATPGKKLLFMGGEIGQWREWDHERSLDW
                     HLLAQAPHAGVQRLVGHLNRTYRSEPAMHTRDCDGGGFEWIDANDAEHSVISFLRWGT
                     DGSVVLVVANFRPMPRPSYVVGVPRGGSWREIVNTDAEEYGGSGQGNLGGVEAVPLSA
                     HGRPRALTITLPPLAAVWFRHEPR"
BASE COUNT          573 a         1392 c         1479 g          644 t
ORIGIN      
        1 gccagtggga cgagcggcat gaaggccgcg ctgaacggcg gtttgaacct gtccgtcctg
       61 gacggctggt gggaggaggc atacgacggg tcgaacggct gggcgatcga gggcgacgtg
      121 gaagcggata tggcggcgca ggacgcccgc gacgccgcga cgctgtacga cctgctcgag
      181 cgcgaggtcg tgccgctgtt ccacgaccgc gacgagcgcg ggatcccgcg gggctggatg
      241 gcccgcgtca aggcttcgct gcggacgatc ggcccgcgct actgcgccgc gcgcatgctc
      301 gacgactacg tccgcgacgt ctacgccgcg cagccggcgg gcgccgccgt acggtagccg
      361 gtccacgagc gccgcggccc cggcccgacg ctagtgctcg gcgaggcgca ggaagatcag
      421 cgagtgggcg gccaggttga cgccgggctt gcgcactacg cgggcgccgg cgcgggcggt
      481 gttcagctcc tcgtgccagc ggccctcgcc gacggcgggg agctggaaca ggcgcgggcg
      541 gtcgcccccg ttcatcagca ggagcacggt gtcgccggag accggccggc cgcgctggtc
      601 gacctcgtcg gtggcccggc cgtggatcag catgccgagc acgtgccggt cctcgtcggc
      661 ccagtcctcg tcggacatct cctgcccgtc gcggcgcagc caggtgacgt ccttcgtccc
      721 gcgcgcgtct atcggccgtc cggtgaagaa cgagcgccgc cgcagcaccg ggttctcgtt
      781 ccgcaggcgg atcagcaggc gggtgtactc gagcagcgac cgttcggcgg tgctcaggtc
      841 ccagcgcacc cacgacagat cgttgtcctg gcagtagccg ttgttgttgc cgcgctgcga
      901 tctgcggatc tcgtccccgt gcgagagcat cggcacgccc tgcgagagca ggagcgtggc
      961 caggaagttg cgcttcgcgc gctcccggag gtggacgacg cgctggttgc cggtctcgcc
     1021 ctcggtgccc cagttggacg agtggttctc gtcggcgccg tcggtgttgt cgaagccgtt
     1081 cgcctcgttg tgcttgcgct cgtaggagac caggtcgtgc agcgtgaagc cgtcgtggca
     1141 cgtgacgaag ttgatcgttg cgtacgtgtg ccgtccgccg gccgcgtaga ggtcgctcga
     1201 cccggagagg cgtgaggcca gctcgctgac cagcccgtcg tcgccgcgcc agaaccggcg
     1261 cacgcagtct cggtactttc cgttccactc cgcccacccg atcgggaagt tccccacttg
     1321 gtacccgccc ggccccacgt cccagggctc ggcgatcagc ttgacctggg agatcgtcgg
     1381 gtcctgctgg atgatgtcga agaacgtccc cagccggttc acctcgtaca gctcgcgcgc
     1441 cagcgccggc gccaggtcga agcggaagcc gtccacgtgc acgtcggtga tccagtagcg
     1501 gagcgagtcc atgatcagct ggatcgtgcg cggatggagc atgttcaggg agttgccgca
     1561 ccccgtgaag tcgaggtaac ggctgcggtc ctcggggtcc agccgatagt aggcggcgtt
     1621 gtccacgccg cgcaggctga gggtcggccc caggtggttc ccctcgccgg tgtggttgta
     1681 gacgacgtcg agcacgacct cgatcccggc gcggtggaac gccttgacca tgctcttgaa
     1741 ctcgtcgacc tgaccgccga gccgcccggc cgcgtagcga acctcggggg cgaggaaggc
     1801 gatcgagttg tacccccagt agttggtcag gccgcgcgcg gacagctcgt gctccgccac
     1861 ggactggtgg acgggcatca gctcgaccgt cgtcacgccg agcgagacca ggtggtcgag
     1921 gacggggtcc tgcgccatgc cgaggtacgt gccgcggagc tcctcgggga tctccgggtg
     1981 gagcatggtc atcccgcgga cgtggctctc gtagatgatc gtccggttcc acgggacggc
     2041 cggtcgccgg tcaccctccc aggcgaaggc cgggtcgatc accatgcact tcggcatcac
     2101 cggcccgctg tcccgctcgt ccggctcgct ctcctcggac ccgggccggt acccgagcag
     2161 cgcgtcgtcc cagcggatcg ggccggcgat cgccttggcg taggggtcga tcagcagctt
     2221 gtgggggttg aaccggtgcc cgtggtccgg cgcgtagggg ccgcggaccc ggtacccgta
     2281 ggccaggccc ggccgcgcgt cggggaggta acagtgccag atcaggtcgg tgcgctcccg
     2341 cagctcgatc cgggcggact cggcgggatc gtcgggcgac tcgtagacgc agagctccac
     2401 cgcctcggcg tgctcggaga agatggcgaa gttgaccccg agcccgtcgt aggtcgcccc
     2461 gagcgggtac ggctctccgg gccagaccct caccgaggct cgtggcggaa ccacaccgcc
     2521 gccagcggcg gcagcgtgat ggttagcgcg cgcgggcgtc cgtgggcgct cagcgggaca
     2581 gcctcgacgc cgccgaggtt gccctgtccg ctgccgccgt actcctcggc gtcggtgttc
     2641 acgatctcgc gccaggatcc gccgcgcggg acgccgacca cgtagctcgg gcgcggcatc
     2701 ggtctgaagt tggcgaccac gagcaccacg gacccgtccg tcccccaacg caggaagctg
     2761 atcacggagt gctccgcgtc gttggcgtcg atccactcga agccgccgcc gtcgcagtcg
     2821 cgggtgtgca tggccggctc gctgcggtag gtgcggttca ggtgcccgac caatcgctgc
     2881 acgccggcgt gcggcgcctg ggccaggagg tgccagtcga gcgagcgttc atggtcccac
     2941 tcccgccact ggccgatctc cccgcccatg aacagcagct tctttcccgg cgtcgcgtag
     3001 agatgggcga gcagcaggcg caggttggcg aaccgctgcc agtcgtcgcc cggcatcttg
     3061 ccgagcagcg acccctttcc gtgcacgacc tcgtcgtggg agagcggcag cacgaagttc
     3121 tcgctcccgg cgtacatcgc gcggaacgtg aggttgccgt ggtggtagcg gcggtggacc
     3181 ggttccctcg ccgcgtagcc gagcgtgtcg ttcatccagc ccatgtccca cttcagcccg
     3241 aagccgagcc cgccgacgtg ggtcggccgc gacaccatcg gccaggccgt cgactcctcg
     3301 gcgatggtct gcacgtccgg gtgggcccgg tagacgtcct cgttgaagcg gcgcaggaac
     3361 ccgatcgcct ccaggttctc gttgccgccg tactcgttcg ggatccactc gccgggcccc
     3421 cgcgagtagt cgaggtagag catcgaggcg acggcgtcca cccgcaggcc gtcggcgtgg
     3481 tacgcgtcga gccagtgcat cgcggaggac atcaggaacg agcggacctc gttccgcccg
     3541 tagttgaaga tcgcggagtt ccagtcgggg tgatagccga gcttcgggtc ctcgtgctcg
     3601 tagaggtgcg atccgtcgaa gaacaccagg ccgtgctcgt cggtggggaa gtgcgagggc
     3661 acccagtcca ggatcacgcc gatcccggcc cggtgcagct cgtcgatcag gtccatcagg
     3721 tcctgcggct tcccgtatcg ggcggtgggg gcgaagtacc cggtggtctg atagccccac
     3781 gagccgtaga aggggtgctc cgtgatcggc aggaactcga cgtgggtgaa gccctgctcg
     3841 gcgcagtacg ccgagaggcg cggggcgagc tcgcggtagg tgagccagcg gtcgccgtcc
     3901 tccggcgcgc gcatccacga gccgaggtgc acttcgtaga tcgccatcgg cgagtccagg
     3961 ctctgccggc gggcccgggt gcgcatccac tccccgtcgc cccactcgtg gtcgaggtgc
     4021 cagacgatcg agccggtccg cggcgggacc tcggcgcgca gcgcgaaggg atcggccttg
     4081 tcgacgcg
//
LOCUS       DASPWO010000520         7038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_155201, whole genome shotgun sequence.
ACCESSION   DASPWO010000520 DASPWO010000000
VERSION     DASPWO010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155201"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VEO00_11920"
     CDS             complement(<1..273)
                     /locus_tag="VEO00_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58740.1"
                     /translation="MARRVTSTRALVAVLAVAVVTLAACSTSSAPKAAAGLQARKTTI
                     GGLEVTVTPTELDSRGAAFTVVFDTHNGAPGIDVAANAALDVDGTAW"
     gene            complement(258..719)
                     /locus_tag="VEO00_11925"
     CDS             complement(258..719)
                     /locus_tag="VEO00_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58741.1"
                     /translation="MTGRSIRIGALGASALSAFYIAVIGWASGADHLATQAPADWPYL
                     TIIIAGFGTQLALLAELRHRHRARHLEQAAGGAGAGASAVGMVACCAHHIADLVPVVG
                     ASGAAAFLTDWRTEFMLAGIAISAAGVAVAARRLVADSRYPIAKGPAWHAA"
     gene            complement(716..964)
                     /locus_tag="VEO00_11930"
     CDS             complement(716..964)
                     /locus_tag="VEO00_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HYU58742.1"
                     /translation="MMHSSRYFPYLVGAAAVAAVLIAVGAPLASLLPFAIVLACPLMM
                     IFMMKGMAGMHGGGEDHTGHGCEHDPTRTAEPPVSHQR"
     gene            1105..3783
                     /locus_tag="VEO00_11935"
     CDS             1105..3783
                     /locus_tag="VEO00_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HYU58743.1"
                     /translation="MTVAQIVVTVAGVVVIAVLTRFFFRARAAADAVEVAGVQEVRVV
                     VRGGYSPDVIRARTGAPLRIVFDRQEDGDCSSRVVFTDLALSRFLPPFAETTVELTPS
                     HAGTYGFACGMNMIHGTLLIEGDPALGDSSAGGIAVAEENAVIGGDGEDVEAAARRAE
                     VTDLSRRLAVGAVLTAPVLFAVMGRDVFGFDWIPMWLMNRWLQLALIAPVMVWVGWPI
                     HRTGWLALAHRAADMNSLITLGTVAAFGYSTVVTVAPNVVPADVRDVYFEAVGVIITL
                     ILLGRLLEARAKAGTGEAIRRLIGLQARTARVERDGATVEVPVEEVVAGDVVLVRPGE
                     KVPVDGEIVDGRSSLDESIVTGELMPVTKETGDVVIGATLNQTGAFCMRATKVGRDTM
                     LAQIVRLVRQAQSSKAPIQRLADAVARWFVPAVLFIAVATFVVWFDVGPTPAFQFALV
                     AAVAVLIIACPCALGIATPLSIMVATGKGAEAGVLIRSAEALETAHKLDVLILDKTGT
                     LTKGHPDLTDIVTTPGFDDDELLTLVAAAEARSEHPLAAAVVAAAHDRSLTVADADEF
                     DSVTGKGVGATIGGRRVLVGNPRLLDDSGIDPAPLADAAATLAADGKTPMLVAVDGLT
                     AGVVAVADTLKDDAAASVAALRRLGLEVVMLTGDNRRTAEAIARATGVDRVLAEVLPG
                     DKAAEVARLQGEGRTVGMVGDGINDAPALAQADVGIAIGTGTDVAIEAADITLISGQL
                     AGLVTAIALSKATMRNIRQNLGFAFGYNALGIPLAAGVLYPFIGWRLNPMIAAAAMAA
                     SSLSVVTNANRLRRFHPPAAAPTPAAPVAAVIVDTPPAQAIDPVCAMTVTAAPPTLSF
                     TADGRTWWFCSAGCRDTVAADPAAFIADHAAAGGAR"
     gene            3780..3935
                     /locus_tag="VEO00_11940"
     CDS             3780..3935
                     /locus_tag="VEO00_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58744.1"
                     /translation="MMWGHYDEFSWWWFLLMPLGMVGFWALVAWVIVTVVRGDRRDQP
                     PPASKSG"
     gene            3939..5039
                     /locus_tag="VEO00_11945"
     CDS             3939..5039
                     /locus_tag="VEO00_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU58745.1"
                     /translation="MGRVPLARRNLLADRRRLAASVTGVGLAVMLILLLDGMWAGIRQ
                     QTTLYTDRTGADLYVLQPGVRDLTAGASTLPLTTLDTVRADPGVDWVAPVRTTYSIFQ
                     LHGTKVAAYVVGAVPGEPGGPWSITRGRAPQADDEIVVGSLLADRHGIHVGDRLDVMG
                     HGLRVVGLAETNGFMMSYVFVTHAALDALAVTPNTTSFLLVGTRQPAAVQARLQRGGL
                     NVLARDEVAANNLRFATGIFGSPVRLMVGIGLAAGTMIIALTAYTAIIERRREYGIVK
                     AMGGTRRYLVALALGQTLALAGLGLVAGWVFFVAGRLLIVSTRPQFTVLLTGGAVGRA
                     ALAALAMALLAAVIPARRLAALEPAVAYRSAT"
     gene            5036..6154
                     /locus_tag="VEO00_11950"
     CDS             5036..6154
                     /locus_tag="VEO00_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYU58746.1"
                     /translation="MIGRVPLARRALFQDRRRAALAAGGVAAALLLVLMLQGIFDGAM
                     HQVTAYLRANPADVIVSQRDVRTMHMSASALDPATVQSVAAVDGVAWAEGIRYTTTFL
                     VGGDGNQQLSYVIGYDTTTGRGGPQHLTAGRAPGGDEIVVEQVAADQLGLHVGDTVSV
                     FGDRLRVSGVFRGGTSVVNSIAFITTDDFAARRGPAVAYILAGAKPGVSADELQRRLA
                     AALPELTVQTRSRFTREEAALVRDMSADLMRIMSIIGLLIALAVIGLTLFTLTLSKLR
                     EHAIVKALGGRTRRLAAVVLAQAAWSVGVAVVAATTLAIALGTVVERANPAITIVIEP
                     GSVERIAVAALVIGAIGAVIPLRRVVAVDPASAFRRAS"
     gene            6151..6984
                     /locus_tag="VEO00_11955"
     CDS             6151..6984
                     /locus_tag="VEO00_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYU58747.1"
                     /translation="MTNLSVHDLTKTYGQGELAVAAVRHVDLDVEPGEVVLIMGPSGS
                     GKTTLLLMLGAMLRPTSGTITVDGVDLATAPERALPPLRAGHFGFVFQDFNLLSALTA
                     LENVELACKLAGVHGRPARQRATDLLTRLGLSERLRFRPDQLSGGEKQRVAIARALAN
                     NATVILADEPTANLDSAHGREIARQLRQLATDDGRSVVIVSHDDRLREIADRVLWLED
                     GTFRELAAMTIDPVCGMPVDTTSPYRHDHDGRSWWFCSAHCRDEFTGDPERFETEAAS
                     P"
BASE COUNT          973 a         2694 c         2324 g         1047 t
ORIGIN      
        1 ccatgcggtg ccgtcgacgt cgagggcggc gttggcggcg acgtcgatgc cgggcgcgcc
       61 gttgtgggtg tcgaacacga ccgtgaacgc ggcgccgcgg ctgtcgagct cggtgggggt
      121 gacggtcact tcgagcccgc cgatcgtggt cttgcgggcc tgcagtccgg ccgccgcctt
      181 cggggcgctc gacgtggagc acgcggccag ggtgacgacg gcgacggcca gcaccgctac
      241 gagcgcccgc gtcgacgtca cgcggcgtgc catgccggtc ctttcgcgat cgggtagcgg
      301 gagtcggcga caaggcggcg ggcggcgacc gcgacgccgg cggcgctgat ggcgatgccg
      361 gcgagcatga actcggtgcg ccagtcggtc aggaacgcgg cggcgccgct cgcgccgacg
      421 acggggacaa ggtcggcgat gtggtgggcg caacaggcga ccatgccaac cgcggacgcg
      481 ccggcgcccg cgccgccggc ggcttgttcg aggtggcggg cgcggtgccg gtgacggagc
      541 tcggcgagca gggcgagctg ggtgccgaac ccggcgatga tgatggtgag atacggccag
      601 tccgccgggg cttgggtggc gaggtggtcg gcgcctgatg cccagccgat gaccgcgatg
      661 tagaaggcgg aaagcgcact ggcaccgagg gcgccgatgc ggatgctgcg cccggtcacc
      721 gctggtggct gacgggcggc tcggccgttc gggtcgggtc gtgctcgcag ccatggccgg
      781 tgtggtcctc gccgccgccg tgcatgccgg ccatgccctt catcatgaag atcatcatga
      841 gcgggcaggc gagcacgatc gcgaacggaa gcagcgaagc gagcggcgct ccgacggcga
      901 tcagcacggc cgcgacagcc gcggcgccaa ccaggtaagg aaagtatcga ctcgagtgca
      961 tcatcagggc ctcctcgata ggtgcctctg actgcaggat cgacctttcg tgcggccgcg
     1021 aacagagtcg aaagtcccta cccctagggg gtatttgcgg cgcacagtgg tgatgcggcc
     1081 gaaacgtcgc aggaggtggt tgtcatgacc gttgcgcaga tcgtcgtcac ggtcgccggc
     1141 gtggtggtga tcgccgtgct gacccgcttc ttcttccggg cgcgcgctgc tgccgacgcg
     1201 gtcgaggtgg ccggcgtgca ggaggtccgg gtagtggtcc ggggcggcta cagccccgac
     1261 gtgatccgag cccgcaccgg tgcgccgctg cggatcgtgt tcgaccgcca ggaggacggc
     1321 gactgctcat cgcgagtggt gttcaccgac ctggcgctga gccgcttcct cccaccgttc
     1381 gccgagacga ccgtcgagct cacgccgagc catgccggca cctacgggtt cgcctgcggc
     1441 atgaacatga tccacgggac gctgttgatc gaaggcgacc cggccctcgg tgacagctcc
     1501 gccggcggga tcgccgtggc ggaggaaaac gcggtgatcg gcggcgacgg tgaggacgtc
     1561 gaagccgccg cccgccgggc cgaggtcacc gacctgtcac ggcggctggc cgtcggcgcc
     1621 gtactcaccg cgccggtgct gttcgcggtg atgggccgcg acgtgttcgg gttcgactgg
     1681 atcccgatgt ggctgatgaa ccgctggctg cagctggcgc tgatcgcgcc agtgatggtg
     1741 tgggtcggct ggccgatcca ccgcaccggc tggctcgccc tcgcccaccg cgccgcggac
     1801 atgaacagcc tcattaccct cggcaccgtc gccgcgttcg gctacagcac cgtcgtcacc
     1861 gtcgccccga acgtcgtgcc ggccgacgtg cgcgacgtct acttcgaagc ggtcggagtg
     1921 atcatcacgc tcatcctgtt gggccggctc ctcgaggcgc gcgccaaggc cggcacgggc
     1981 gaagccatcc gccgcctcat tggcctccaa gcgcgcaccg cccgcgtgga acgtgacggc
     2041 gccaccgtcg aggtgccggt cgaggaggtc gtcgccggcg atgtcgttct cgtacgcccg
     2101 ggtgagaagg tgcccgtcga cggcgagatc gtcgacgggc ggtcgagcct cgatgagtcg
     2161 atagtcaccg gcgagttgat gccggtgacc aaggagacgg gcgacgtggt gatcggcgcc
     2221 accctcaacc agaccggcgc gttctgcatg cgggccacca aggtcgggcg cgacacgatg
     2281 ctcgcccaga tcgtgcgtct cgtgcgccaa gcccagtcgt ccaaggcgcc catccagcgg
     2341 ctcgccgacg cggtggcgcg ctggttcgtt cccgcggtgc tgttcatcgc ggtggcgacc
     2401 ttcgtcgtgt ggttcgacgt cggaccgacg ccggcgttcc agttcgcgct ggtcgccgcg
     2461 gtcgccgtgt tgatcatcgc ctgcccgtgt gcgctcggca tcgccacgcc gctgtcgatc
     2521 atggtcgcga ccggcaaggg cgctgaggcg ggcgtgctca tccgctccgc cgaagcgctc
     2581 gaaacggcgc acaagctcga cgtgctgatc ctcgacaaga cgggcacgct gaccaagggc
     2641 caccccgatc tcaccgacat cgtcaccacc cccggcttcg acgacgacga gttgctcacg
     2701 ttggtggcgg cagccgaggc ccgctccgag cacccactcg ccgctgccgt cgtggccgcc
     2761 gcccacgacc gttcgctcac cgtcgccgac gccgacgagt tcgactcggt gaccggcaaa
     2821 ggcgtcggcg ccaccatcgg cgggcgacgc gtgctcgtcg gcaacccccg cctcctcgac
     2881 gattctggca tcgaccccgc accgctggcg gacgcggccg ccacgctggc ggccgacggc
     2941 aagaccccga tgctggtggc cgtcgatggg ttgaccgccg gtgtggttgc cgtggcggac
     3001 accctcaaag acgacgccgc tgccagcgtc gccgcgctgc gccgtctcgg gctcgaggtc
     3061 gtgatgctca ctggcgacaa ccggcgcacg gctgaggcca tcgcccgcgc caccggcgtc
     3121 gaccgggtcc tggccgaggt gctccccggt gacaaggccg ctgaggtcgc ccgcctccag
     3181 ggcgaaggcc gcactgtcgg gatggtcggt gatggcatca acgacgcgcc ggccctcgcg
     3241 caggccgacg tcggcatcgc catcggcacc ggcaccgacg tggcgatcga agccgccgac
     3301 atcaccctca tctcgggcca actcgccggg ctcgtgaccg ccatcgcgct gtccaaggcg
     3361 acgatgcgca acatccgcca gaacctcggc ttcgcgttcg gctacaacgc gctgggcatc
     3421 cccctcgccg ccggcgtgct ctatccgttc atcggctggc ggctcaaccc catgatcgcc
     3481 gccgccgcca tggccgcgtc cagcctgtcg gtggtcacca acgccaaccg gctccgccgc
     3541 ttccacccgc cggccgccgc accgacacca gcagcgccgg tcgccgccgt gatcgtggac
     3601 accccgccgg cccaggcgat cgacccggtg tgcgccatga ccgtgaccgc agcaccgcca
     3661 accctgtcgt tcaccgccga cgggcgcacc tggtggttct gctcggccgg ctgccgcgac
     3721 accgtcgccg ccgacccggc cgcgttcatc gccgaccacg ccgccgcagg tggtgcccga
     3781 tgatgtgggg tcactacgac gagttcagct ggtggtggtt cctgctcatg ccgctgggca
     3841 tggtcggctt ctgggccctc gtcgcctggg tgatcgtcac cgtcgtccgc ggtgatcggc
     3901 gagaccagcc accaccggca tcgaagtcgg gctgacccat gggccgcgtc ccgctcgccc
     3961 gccgcaacct cctcgccgac cgacgtcgac tggcggcgag cgtcaccggc gtcgggctcg
     4021 ccgtcatgtt gatcctgctg ctcgacggca tgtgggccgg catccgccag cagaccaccc
     4081 tctacaccga ccgcaccggc gccgacctct acgtgctgca accgggcgtg cgcgacctca
     4141 ccgccggggc cagcaccctg ccgctcacca ccctcgacac ggtgcgagcc gatccgggcg
     4201 tcgattgggt ggcaccggtg cgcaccacct attcgatctt ccagctgcac ggcaccaagg
     4261 tggcggccta cgtcgtcggc gccgtgcccg gcgagccggg cggaccatgg tcgatcacac
     4321 gcgggcgtgc cccgcaggcc gacgacgaga tcgtcgtcgg gtcactcctc gccgaccgtc
     4381 acggcatcca cgtcggtgac cggctcgacg tcatgggtca cggcctgcgc gtcgtgggtc
     4441 tcgccgagac caacgggttc atgatgtcct acgtcttcgt cacccacgcc gcgctcgacg
     4501 cgctggcggt gacgccgaac accacgagct tcctgctcgt cggcacccgc cagccggccg
     4561 ccgtccaagc gcggctgcaa cgcggcggac tgaacgtgct ggcccgtgac gaggtggccg
     4621 ccaacaacct caggttcgcc actggcatct tcggttcacc ggtgcggctc atggtcggca
     4681 tcggcctcgc cgccggcacg atgatcatcg ccctcaccgc ctacaccgcc atcatcgaac
     4741 ggcgacgcga gtacgggatc gtcaaggcca tgggcggcac ccgccgctac ctcgtggcgc
     4801 tcgctctcgg ccagaccctg gcgttggccg ggctcgggct cgtcgccggc tgggtgttct
     4861 tcgtcgccgg ccggctgctc atcgtgtcga cccgccccca gttcaccgtg ctgctcaccg
     4921 gcggcgccgt cggacgcgcc gcgctcgcgg cgctggccat ggcgctgctg gccgccgtca
     4981 tccccgcccg tcggctcgcc gcgctcgagc cggccgtcgc ctaccggagc gccacatgat
     5041 cggccgcgtt cccctggcgc gccgggcgct gttccaggac cgccgccgcg ccgcgctcgc
     5101 cgccggcggt gtcgccgccg cgctcctcct cgtcctcatg ctgcaaggaa tcttcgacgg
     5161 cgccatgcac caagtcaccg cctatctgcg cgccaacccc gcggatgtga tcgtgtccca
     5221 gcgcgacgtc cgcaccatgc acatgtcggc ctccgcgctc gaccccgcca ccgtgcaatc
     5281 cgtcgccgcg gtcgacggtg tcgcgtgggc cgaaggcatc cggtacacga ccaccttcct
     5341 tgtcggcggc gacggcaacc agcagctcag ctacgtcatc ggctacgaca ccaccaccgg
     5401 ccgcggcgga ccacaacacc tcaccgccgg ccgggcccca ggcggcgacg agatcgtcgt
     5461 cgagcaggtc gccgccgacc agctcggcct gcacgtcggc gacacagtct cggtgttcgg
     5521 cgaccgcctc cgcgtgagcg gcgtgttccg gggcggcacc agcgtcgtca actccatcgc
     5581 cttcatcacc accgacgact tcgccgcccg ccgcggaccc gccgtcgcct acatcctcgc
     5641 cggcgccaag ccgggcgtct ccgccgacga actccaacgc cgcctcgccg ccgcgctccc
     5701 cgagctcacc gtgcagaccc ggagccggtt cacccgcgaa gaagccgccc tcgtgcgcga
     5761 catgtcagcc gacctgatgc ggatcatgtc gatcatcggt ctcctcatcg ccctcgccgt
     5821 catcgggctc accctcttca ccctcaccct gtccaagctt cgagagcacg ccatcgtcaa
     5881 ggccctcggc ggccgcactc gccggctcgc cgccgtcgtc ctcgctcaag ccgcctggtc
     5941 ggtcggcgtc gccgtcgtcg ccgccaccac cctcgctatc gcgctcggca ccgtcgtcga
     6001 acgcgccaac cccgccatca ccatcgtcat cgaaccgggc agcgtcgagc gcatcgccgt
     6061 cgccgccctc gtgatcggcg ccatcggcgc cgtcatcccc ctgcggcgcg tcgtcgccgt
     6121 cgaccccgca tccgcgttcc ggagggcgtc atgaccaacc tgtccgtcca cgacctgacc
     6181 aagacctacg gtcaaggcga actcgccgtc gccgccgtcc gtcacgtcga cctcgacgta
     6241 gagcccggcg aggtcgtgct catcatgggc ccctccggca gcggcaagac caccctcctc
     6301 ctgatgctcg gcgccatgct ccgccccacc agcggcacca tcaccgtcga cggcgtcgac
     6361 ctcgccaccg cccccgaacg ggcgctgccg ccgcttcggg ccggccactt cgggttcgtg
     6421 ttccaggact tcaacctcct ctccgccctc accgcgctcg agaacgtcga gctcgcctgc
     6481 aagctcgccg gcgtccacgg acgccccgcc cgccagcgcg ccaccgacct gctcacccgg
     6541 ctcgggctga gcgaacggct gcggttccgg ccggaccagc tctccggtgg cgagaaacag
     6601 cgggtcgcca tcgcccgcgc cctcgccaac aacgccaccg tcatcctcgc cgacgagccc
     6661 accgccaacc tcgactccgc ccacggccgc gagatcgccc gccagctccg ccagctcgcc
     6721 accgacgacg gccgcagtgt tgtcatcgtc tcccacgacg accgcctccg cgagatcgcc
     6781 gaccgtgtcc tgtggctcga agacggcacc ttccgcgagc ttgccgccat gaccatcgac
     6841 cccgtgtgcg gcatgcccgt cgacacgacc tccccctacc gccatgacca cgacggccgc
     6901 agctggtggt tctgctccgc ccattgccgc gacgagttca ccggcgaccc cgagcggttc
     6961 gagaccgagg cggcatcgcc atgagcggaa gcgagcgggt ccgccatccg atcttcgccc
     7021 gcatgtatca gcgcgtcg
//
LOCUS       DASPWO010000521         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_841504, whole genome shotgun sequence.
ACCESSION   DASPWO010000521 DASPWO010000000
VERSION     DASPWO010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841504"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VEO00_11960"
     CDS             <1..237
                     /locus_tag="VEO00_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58748.1"
                     /translation="HTRSERFLRETRDAVRLGHVYGPCYHAHRQYVLWHITRLEELAD
                     LCRMLDVDNFAARCDWVGWRYGRMRDALRYRIAR"
     gene            complement(293..901)
                     /locus_tag="VEO00_11965"
     CDS             complement(293..901)
                     /locus_tag="VEO00_11965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HYU58749.1"
                     /translation="MANLLEVLEEARARGLLGPGPLDEHIGHSRALAGLVGEPPRRFL
                     DLGSGGGIPGLVLAAEWPAAAGVLLDAHRRAQAFLEEAIDRLGLAGRLRAVAGRAEEL
                     ARDPELRSASDLVVARAFGPPPATAECGAGFLREGGRLVVSEPPEPDPARWDPEGLAR
                     LGLSAPAVRRGDGVTIAVMTLEHPVDDRWPRRTGIPSKRPCW"
     gene            complement(917..1810)
                     /gene="jag"
                     /locus_tag="VEO00_11970"
     CDS             complement(917..1810)
                     /gene="jag"
                     /locus_tag="VEO00_11970"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HYU58750.1"
                     /translation="MEWVETTGRTLAEAVDAALDQLGVAEEDLEYQVLDEPKSGLLGR
                     LGGREARIRARVKPLSREKPGDRRRRDRKTRPERGAGRGRRPSGDRGRGRGDGEEPAA
                     ARSGGERSSGSRSRRRRPRRPAEEKQAARSATDEGARVEESEIPIEEQAETAGGFTRG
                     LVEAFGADADVDARIEDGEMILVDVTGENLGLLVGPRGATLSALEELVRTVVQRHTGG
                     HGARINVDVGGYRAKRRQALSEFTQELARKVLDSGQEQALEPMSASDRKVAHDAVAEM
                     DGVTTGSEGEEPRRRVVIRPA"
     gene            complement(1811..>2107)
                     /locus_tag="VEO00_11975"
     CDS             complement(1811..>2107)
                     /locus_tag="VEO00_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58751.1"
                     /translation="APPADAAIPETPAPAQSPKAASPKATSPPEPEPEAPAAAPSGGG
                     RLRGLFGLPAAPNGEPAPARPAAAGKATAAGAARGGSASRSNQGRRRRNKKRKR"
BASE COUNT          232 a          859 c          724 g          292 t
ORIGIN      
        1 cacacccgta gcgagcgctt tctgcgcgag acgcgagacg ccgtgcgcct cggccacgtg
       61 tacgggccgt gctatcacgc ccatcggcag tacgtgctgt ggcatatcac tcgcctcgag
      121 gagctcgccg acctctgccg gatgcttgac gtcgacaatt tcgcggcccg ctgtgactgg
      181 gttgggtggc ggtacggtcg gatgcgggac gccctgcgat accgcatcgc ccggtaggcg
      241 tctcccggga tgttccacgg tgaacacccc tggtgatgtt caccgtggaa catcaccagc
      301 aggggcgctt cgacgggatg ccggtgcggc ggggccaccg gtcgtcgacc gggtgctcca
      361 acgtcatcac cgcgatggtc acgccgtcgc ctcggcggac ggccggcgcc gacaggccca
      421 ggcgggcgag tccctccggg tcccatctcg ccgggtccgg ctccggaggc tcgctcacga
      481 cgagccgtcc gccctctcgc aggaagccgg ccccgcattc agccgtcgcc ggcggcggac
      541 cgaaggcccg ggcgacgacc aggtccgagg cgctccgcag ctcgggatcc cgggccagct
      601 cctccgcccg gcccgcgacc gcccgcagcc gtcccgccag ccccaggcgg tcgatcgcct
      661 cctcgaggaa ggcctgcgcc cgccgatgcg cgtcgagcag cacgccggcg gccgccggcc
      721 actccgccgc caggacgagg cccgggatgc ccccacccga tccgagatcg agaaaacgcc
      781 ggggcggctc gccgaccagg cccgccagcg cccggctgtg gccgatgtgc tcgtcgagcg
      841 gcccggggcc gaggaggccg cgggctctgg cctcctccaa cacctcgagg aggttcgcca
      901 tccgccctcc gaacgactac gccgggcgga tgacgacccg gcgccggggc tcctcgccct
      961 cggagccggt ggtgaccccg tccatctcgg cgacggcgtc gtgggcgacc ttgcggtccg
     1021 acgccgacat gggctccagg gcctgctcct ggccgctgtc gagcaccttg cgggcgagct
     1081 cctgggtgaa ctcggagaga gcctggcggc gcttggcccg gtacccgccg acgtcgacgt
     1141 tgatccgggc gccgtggccg ccggtatggc gctggacgac ggtgcgcacg agctcctcca
     1201 gcgcgctcag ggtcgccccc cgcgggccca ccagcaaccc caggttctcg ccggtcacgt
     1261 ccacgaggat catctcgccg tcctcgatcc gcgcatcgac gtcggcgtcg gcgccgaacg
     1321 cctcgaccag gccgcgggtg aaccccccgg ccgtctcggc ctgctcctcg atgggtattt
     1381 cggattcctc caccctcgct ccctcgtccg tggcggatcg cgccgcctgc ttctcctcgg
     1441 ccggtcgccg cggtcggcgc cggcgcgatc tcgacccgct cgaccgctca ccgcccgacc
     1501 gggcggcggc gggctcctcc ccgtccccgc ggcctcgacc tcggtccccg ctcggccggc
     1561 ggcccctccc ggcgccgcgc tccgggcggg tcttgcggtc acgccgccgg cgatcgccgg
     1621 gcttctcacg cgagagcggc ttcacccggg cccggatgcg ggcctcccgg ccgccgagcc
     1681 gacccagcag accgctcttg ggctcgtcga ggacctggta ctcgaggtcc tcctccgcca
     1741 cccccaactg gtccagcgcc gcgtcaaccg cctccgcaag cgtccgaccc gtcgtctcta
     1801 cccactccat ctaccgcttc cgcttcttgt tgcgccggcg ccgaccctgg ttcgagcggc
     1861 tcgcgctccc gccccgcgcc gctcccgccg cggtggcctt tcccgcggcc gccgggcgcg
     1921 ccggggcagg ctctccgttc ggggccgccg gcagaccgaa caggccccgc agccgcccgc
     1981 cccccgaggg ggccgcggcc ggcgcctcgg gctcgggttc cggcggagac gtcgccttcg
     2041 gggaggccgc cttcggagat tgggccggcg ccggcgtctc ggggatcgcc gcatcggccg
     2101 ggggcgc
//
LOCUS       DASPWO010000522         5173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_414938, whole genome shotgun sequence.
ACCESSION   DASPWO010000522 DASPWO010000000
VERSION     DASPWO010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414938"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VEO00_11980"
     CDS             complement(<1..507)
                     /locus_tag="VEO00_11980"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HYU58752.1"
                     /translation="MRPTPETLHASERDRRAVEEALGKPPDIPFEVVARCPDGAPLVI
                     RDAVRDPAGAPFPTRYWLACPEAVRSVSRLEAAGWIGRLNRRAESDPGFGAALSAAHA
                     AYARERERAEPGAGAWGGVGGTRRGVKCLHAHLAYHLAGGADPVGAWTAERTDPWHPP
                     PATPPAAAV"
     gene            complement(504..1016)
                     /locus_tag="VEO00_11985"
     CDS             complement(504..1016)
                     /locus_tag="VEO00_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HYU58753.1"
                     /translation="MPASATRRSRTTASAGSSLSSHRPVDAPTGGWDSRDAAHGEPPC
                     CRKGADISDLEVGAVVEGTVQRITPYGAFLQLDGGQIGLVHISEIDRNYVRDVREHLR
                     ENDRVQAKVVAIKEDGKIDLSIKALQDPAPRLERRGKDPGFESMLKKFMRQSEERQVD
                     FKRSLDHKRR"
     gene            complement(955..2157)
                     /locus_tag="VEO00_11990"
     CDS             complement(955..2157)
                     /locus_tag="VEO00_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HYU58754.1"
                     /translation="MSATFVPDQLWEQSTPGVENVAEGNDHFGKALASGDFDGDGFDD
                     LTVGVSENFGGLADPGAVNVLYGSDEGLVADDLPSTRADQFFTQDSPNVEGVAEDGDF
                     FGRSLAAGDFNGDGFDDLAIGAPGEDLGNVGDAGAVHVLYGVGAGLSPTTTPNDELWH
                     QDVPGIGSSGEEGDWFGGALAAGDFDADGLDDLAIGVPDEGLDDAPVDRAGIVQVLYG
                     ASHNGGLGSAGAQLWAQGVGGVNDQSEADGLFGWALASANFGNGGQDDLAIGVYGEDV
                     QGLAGAGAVNVIYGSAHGLTTAGIADQFWTQASNDVGGEPGVQDRFGGALAAFDFGGS
                     GHADLAIGAQLEDSSGQTDAGAVNVIYGSPNGLSATGTPDDLWHQDNASVGDSAEPYD
                     RFGGKLPL"
     gene            complement(2173..2610)
                     /locus_tag="VEO00_11995"
     CDS             complement(2173..2610)
                     /locus_tag="VEO00_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58755.1"
                     /translation="MKRAPTLAILALLVWVLSTPIPLARGLPLGAATPAGGAGGEADF
                     DGDGFSDLAIGAVDEDVGNLSDAGAVNVIYGDDGGLSPALSPNEVWTQNSLNVAGAAE
                     PGDQFGWALAIENFNDDAYDDLAIGVPDEDLGDIPNAGAVNVI"
     gene            complement(2694..3017)
                     /locus_tag="VEO00_12000"
     CDS             complement(2694..3017)
                     /locus_tag="VEO00_12000"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HYU58756.1"
                     /translation="MHAARIAGRRARVTLRALVLLVAVLAMLLAISYPLRQYVDARGD
                     VARLEQRLSGLEEQNRTLHEQIERLHDPEYVERLARECLGMVRPGEIAFVVAPEGGEA
                     EPPAC"
     gene            complement(3037..4314)
                     /gene="eno"
                     /locus_tag="VEO00_12005"
     CDS             complement(3037..4314)
                     /gene="eno"
                     /locus_tag="VEO00_12005"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HYU58757.1"
                     /translation="MPAITSLSAREILDSRGNPTVEVDVFLEDGSAGRAAVPSGASTG
                     TFEAVEVRDGDERFGGKGVLHAVRHVEETLGPELIGHDALAQREIDGIMADLDGTPNK
                     AKVGANAILGVSLAVAHAAAQSLGLPLYRYLGGPGAHLLPVPQLNVINGGVHADNDLE
                     PQEFMLAPVGAASFSEALRWGAEVYHALHALLKERTLATGVGDEGGFAPQIATAAEAL
                     DLLVAAIVSAGLEPEREVAIALDPAASEFFSQGAYRLEGKARSSEDMIAFYRGLLDAY
                     PIVSIEDPLAEDDWDGWTAITEVLGGRVQLVGDDLFVTNPERLARGIAARSSNAILIK
                     LNQIGTLSETLDVIGMARASAFGIVVSHRSGETEDSTIADLVVAVNAGQIKAGAPARS
                     ERTAKYNQLLRIEEALGDSARYAGGEPYRRYAR"
     gene            complement(4327..5115)
                     /gene="mazG"
                     /locus_tag="VEO00_12010"
     CDS             complement(4327..5115)
                     /gene="mazG"
                     /locus_tag="VEO00_12010"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HYU58758.1"
                     /translation="MSAGSNATGEPLLELVRVMRRLRDPGGCPWDREQTHASLRRHLL
                     EEAHEVLEAIDAGDDDRLREELGDLLIQVVFHAQIAEDEGAFTVDDVATKLVEKLVRR
                     HPHVFGDARADTPDAVLANWDRIKADEKGGLEEHLEDDIPPSLPALLRAYKVQRRARA
                     RGFDWRSREGALGKLREELGELAGAEGAEEVEAELGDLLFAAVAVARLSGVDAESALR
                     GATRRFAERFEQMLRRADEAGADLAGMSEAELLAFFRRSREGAG"
BASE COUNT          675 a         1962 c         1800 g          736 t
ORIGIN      
        1 gaccgccgcg gccggtggcg tcgccggcgg cgggtgccag gggtccgtgc gctcggcggt
       61 ccaggccccc acgggatcgg cgccgccggc caggtggtag gccaggtggg cgtgcaggca
      121 cttcacaccc cggcgcgtcc cgccgacgcc cccccacgcc cccgcgccgg gctcggcgcg
      181 ctcgcgctcc cgggcgtacg cagcgtgggc cgcgctcagc gcggcgccga acccggggtc
      241 ggactccgcc cgccggttca gccgcccgat ccagcccgcc gcctccaggc gcgacaccga
      301 ccggacggcc tccgggcagg cgagccagta gcgagtcggg aacggggcgc cggcggggtc
      361 gcggaccgcg tcgcggatga ccaggggagc gccgtcggga cagcgcgcga cgacctcgaa
      421 cgggatgtcg ggcggcttgc cgagcgcctc ctcgaccgcg cggcggtcgc gctcggaggc
      481 gtggagcgtc tcgggcgtgg gcctcagcgc cgcttgtgat cgagcgagcg cttgaagtcg
      541 acctggcgct cctcgctctg gcgcatgaac ttcttcagca tcgactcgaa gccggggtcc
      601 ttgccccgcc gctcgagccg cggggccggg tcctgcagcg ccttgatcga caggtcgatc
      661 ttgccatctt ccttgatcgc cacgaccttg gcctgcaccc ggtcgttctc gcggaggtgc
      721 tcccggacgt cgcggacgta gttccggtcg atctcggaga tgtgcaccag cccgatctgt
      781 cccccgtcca gctgcaggaa cgcgccgtac ggcgtgatcc gttgaacggt cccctcgacg
      841 accgctccga cctcgagatc cgagatgtcc gctcctttcc gacagcaagg cggctctccg
      901 tgagccgcgt cgcgactatc ccacccgccc gtcggagcgt caacaggacg gtggctagag
      961 agggagcttc ccgccgaagc ggtcgtacgg ctccgccgag tcgccgacgc tggcattgtc
     1021 ctgatgccag aggtcgtcgg gggtaccggt cgccgacaac ccgttcgggg acccgtagat
     1081 gacgttcacg gcaccggcgt ccgtctgacc cgacgagtcc tcgagctggg cgccgatggc
     1141 caggtcggcg tggccgcttc cgccgaagtc gaacgccgcc aaggccccgc cgaagcggtc
     1201 ctgcacgccg ggctcgccgc caacgtcgtt cgacgcctgg gtccagaact ggtccgcgat
     1261 gcccgcggtc gtcaggccat gggcggaccc gtagatcacg ttcaccgcgc cggcgccggc
     1321 caggccctgg acgtcctcgc cgtagacgcc gatggccagg tcgtcctgcc ccccgttccc
     1381 gaagttggcc gacgccagcg cccatccgaa gagcccatcg gcctccgact ggtcgttcac
     1441 gccgccaacc ccctgtgccc acagctgcgc tccggcgctg ccgagcccac cgttgtgcga
     1501 ggccccgtac agcacctgca cgatgcctgc ccggtccaca ggtgcatcgt cgagaccctc
     1561 gtccggcacg ccgatggcga ggtcgtccag gccgtccgcg tcgaagtctc ccgccgcgag
     1621 cgcgccaccg aaccagtcgc cctcctcgcc ggaactgccg atgccgggca cgtcctggtg
     1681 ccagagctcg tcgttcggcg tcgtcgtcgg cgacaggccg gcgccgaccc cgtacagcac
     1741 gtgcaccgca ccggcatcgc ccacgttccc gaggtcctcc cccggagcgc cgatggccag
     1801 gtcgtcgaag ccgtcgccgt tgaaatcccc ggccgccagc gagcggccga agaagtcgcc
     1861 gtcctcggcg acgccttcca cgttcgggct gtcctgcgtg aagaactgat ccgcccgggt
     1921 gctcggcagg tcatcggcca cgagcccctc gtccgagccg tacagcacgt tcaccgcgcc
     1981 cgggtccgcc agccccccga agttctcact cacgccaacg gtgaggtcgt cgaagccgtc
     2041 gccgtcgaag tcaccgctcg ccagtgcctt cccgaagtga tcgttgccct ccgcgacgtt
     2101 ctcgacgccg ggcgtcgact gctcccagag ctggtccggg acgaacgtgg ccgacagccc
     2161 gctgccggag cgttagatca cgttcacggc gccggcgttc gggatgtcgc cgaggtcctc
     2221 gtcgggaacg ccgatcgcca ggtcgtcgta ggcgtcgtcg ttgaagttct cgatcgcgag
     2281 cgcccacccg aactggtcgc ccggctcggc cgctcccgcg acgttcagac tgttctgcgt
     2341 ccagacctcg ttcggcgaca gcgccggcga gagcccgcca tcgtcgccgt agatcacgtt
     2401 gacggcaccg gcgtccgaga ggttgcccac gtcctcgtcc accgcgccga tcgcgaggtc
     2461 ggagaacccg tcgccgtcga agtcggcctc gccgcccgcc ccgcccgccg gggtcgcggc
     2521 gccgagggga aggccgcgcg cgagcgggat cggcgtagag aggacccaaa cgagcagtgc
     2581 gaggatcgcg agggttgggg cccgcttcat gcagctcgtc ccggtagacg cgattgtgcg
     2641 ccgccccgcc tcgcagggtc aacccgcggg gggacccggg gccccggtgc ccgtcagcag
     2701 gctggtggct cggcttcgcc gccctcgggg gcgacgacga aggcgatctc gccgggacga
     2761 accatgccca ggcactcgcg ggcgagccgc tcgacgtact ccggatcgtg cagccgctcg
     2821 atctgctcgt gcagggtgcg attctgttcc tccaggccgg acagccgctg ctcgagccgg
     2881 gccacgtcgc cccgcgcgtc gacgtactgg cggagcgggt acgagatggc gaggagcatc
     2941 gccagcacgg ccaccagcag gacgagggcg cgaagcgtga cccgcgcccg ccgcccggcg
     3001 atccgcgcgg cgtgcacgct catctcgttc gcgcgctcat cgggcgtacc tccggtacgg
     3061 ctccccgccg gcgtagcgcg ccgagtcgcc gagcgcctcc tcgatccgca gcagctggtt
     3121 gtacttcgcc gtccgctcgg agcgcgccgg ggcgccggcc ttgatctgcc cggcgttcac
     3181 ggcgaccacc aggtcggcga tcgtggagtc ctcggtctcg ccggaccggt gcgagacaac
     3241 gatgccgaag gccgaggcgc gggccatgcc gatcacgtcg agcgtctcgg agagggtccc
     3301 gatctggttc agcttgatca ggatggcgtt cgaggaacgc gcggcgatgc cccgtgccag
     3361 ccgctccggg ttcgtcacga acaggtcgtc gccgacaagc tggacgcggc cgccgaggac
     3421 ctcggtgatc gccgtccagc cgtcccagtc gtcctcggcc agcgggtctt cgatcgacac
     3481 gatcgggtac gcgtcgagca ggccgcgata gaacgcgatc atgtcctcgg acgagcgggc
     3541 cttcccctcc agccggtagg cgccctggct gaagaactcc gacgccgcgg ggtcgagcgc
     3601 gatcgccacc tcccgctccg gctccagccc ggcgctcacg atcgcggcga cgagcaggtc
     3661 gagcgcctcg gcggccgtcg cgatctgcgg cgcgaagccg ccctcgtcgc cgacgccggt
     3721 ggcgagcgtg cgctccttca gcagggcgtg cagggcgtgg tagacctcgg ccccccagcg
     3781 cagggcctcg gagaacgagg cggcgccgac cggggcgagc atgaactcct gcggctcgag
     3841 gtcgttgtcg gcgtggacgc cgccgttgat cacgttcagc tgcgggaccg ggagcaggtg
     3901 cgcccccggg ccgccgaggt agcgatagag cggcaggccg agggactgcg cggcggcgtg
     3961 tgccaccgcg agcgagacgc cgaggatggc gttcgcgccg accttcgcct tgttcggcgt
     4021 gccgtcgagg tcggccatga tcccgtcgat ctcacgctgg gcgagcgcgt cgtgcccgat
     4081 cagctccggg ccgagcgtct cctcgacgtg gcggaccgcg tgcaggacgc ccttcccccc
     4141 gaagcgctcg tcgccgtcgc gcacctcgac ggcctcgaag gtgccggtcg aggcgccgga
     4201 gggcacggcg gcccgcccgg cggagccgtc ctccaggaag acgtccactt cgacggtggg
     4261 attgccgcgg gagtccagga tctcgcgcgc cgacagggaa gtgatggccg gcattgccgg
     4321 gcgatcctac cccgcgccct cgcgcgatct gcggaagaac gccaggagct ccgcctcgct
     4381 catgcccgcc aggtccgctc ccgcctcgtc cgcgcggcgg agcatctgct cgaaccgttc
     4441 ggcgaaccgc cgggtcgcgc cgcgcagggc gctctccgcg tccacgccgg agagccgcgc
     4501 caccgcgact gccgcgaaca gcaggtcgcc cagctcggcc tcgacctcct cggccccctc
     4561 cgctccggcg agctcgccga gctcctcgcg gagctttccc agcgcgccct cgcgcgagcg
     4621 ccagtcgaac ccccgggccc gcgcgcgccg ctgaaccttg taggcgcgca gcagcgccgg
     4681 cagcgacggc gggatgtcgt cctcgaggtg ctcctcgagg ccgcccttct cgtcggcctt
     4741 gatccggtcc cagttggcga gcaccgcgtc gggcgtgtcg gcgcgggcgt cgccgaacac
     4801 gtgcgggtgg cgacggacga gcttctcaac gagcttcgtc gcgacgtcgt cgacggtgaa
     4861 ggcgccctcg tcctcggcga tctgggcgtg gaacaccacc tggatcagca ggtccccgag
     4921 ctcctcgcgc agccggtcgt cgtcgccggc gtcgatcgcc tccagcacct cgtgcgcctc
     4981 ctccagcagg tggcggcgca ggctcgcgtg cgtctgctcc cggtcccagg ggcacccgcc
     5041 cgggtcgcgc agccgccgca tcacccggac gagctcgagc agcggctccc cggtcgcgtt
     5101 cgagccggca ctcatggggt gagctgcatc ggctccgtgg cccggtcctc ggtcgacgtg
     5161 atcgccacga tcc
//
LOCUS       DASPWO010000523         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_497204, whole genome shotgun sequence.
ACCESSION   DASPWO010000523 DASPWO010000000
VERSION     DASPWO010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497204"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VEO00_12015"
     CDS             complement(<1..892)
                     /locus_tag="VEO00_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HYU58759.1"
                     /translation="MEPWSIEFRGTVNEHTITSEALRGNRLGDPHERPLYVYTPPGYD
                     PDGETRYPTIVVIQGLTGQVDMWWNRVAFRTTAMENTDRLFADEGCPPAIVAFVDAWT
                     SLGGSQYVNSPGTGRYLDYLCDEVVPFVDATYRTHGDREHRGISGKSSGGYGAMVVPM
                     LRPDVFAGLATHCGDALFEVCYQPDFREAARVLRDDYEGAFAKFWEDFRSRPALSKKN
                     DGLLLNVWCMAACYSAEEDGTVTLPFDDRTGRLIPEVWERWLALDPVRMAPKHAEALT
                     SMRAIYIDAGKWDEYYLDLGA"
     gene            1013..>2211
                     /locus_tag="VEO00_12020"
     CDS             1013..>2211
                     /locus_tag="VEO00_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HYU58760.1"
                     /translation="MPDVGAVSDVVGIGLAGGQGVRARPLTLKAPGYLRSKAAMAFVG
                     RRLIHWVMQILTAEGIKEYYVVAHGKENRYQIKVLIGYGENLGVDVQYSPVKYDVMSR
                     GSADSTLRLLEHWDIGRPALVFPTDSIIDFDLEPMLLQHLESGAVATIAAMTRPPVEV
                     AGKYGVMLTDPHGKILEFVEKPTLEEIREQFPVTDEPEFDRLPLLTNAGFYLLDAAAL
                     RRIAEDPSLAAVRRNRLDFGMDLLPWLVGNGHPVYAHAVRRLGDLGNIEDYVDTTVDV
                     LHGRFRSIDRLMGEPFDRSRNVWIAPESLHMRDALTGKSLAEKLAEGLVTIGPAVRIG
                     RYCEVGPGVVITESNLDDDVEVREGAYVSRAQVRDGAIIGQAATVSEAVVGSMSEVRS
                     SRGDPTV"
BASE COUNT          331 a          775 c          768 g          337 t
ORIGIN      
        1 cggcgccgag gtccaggtag tactcgtccc acttcccggc gtcgatgtag atcgcgcgca
       61 tcgacgtgag cgcctcggcg tgcttcggcg ccatgcgcac cgggtcgagc gccagccagc
      121 gttcccacac ctccgggatc agccggccgg tgcgatcgtc gaagggcaac gtcaccgtgc
      181 cgtcctcctc ggccgagtag caggccgcca tgcaccacac gttcagcagc aggccgtcgt
      241 tcttcttcga gagcgccggg cgcgagcgga agtcctccca gaacttcgcg aaggcaccct
      301 cgtagtcgtc gcgtaggacc cgcgccgcct cgcggaagtc cggctggtag cagacctcga
      361 acagcgcgtc gccgcagtgg gtggccagcc cggcgaagac gtccgggcga agcatcggca
      421 cgaccatcgc cccgtagccg ccgctcgact tccccgagat gccgcggtgc tcgcggtcgc
      481 cgtgggtccg gtaggtggcg tcgacgaagg ggaccacctc gtcgcacaga tagtccaggt
      541 agcggccggt gcccggcgag ttgacgtact ggctcccccc gagggaggtc caggcgtcca
      601 cgaacgcgac gatcgccggg gggcagccct cgtcggcgaa caggcggtcg gtgttctcca
      661 tcgcggtcgt ccggaacgcc acccggttcc accacatgtc cacctggccg gtcaggccct
      721 ggatgacgac gatcgtcggg tagcgcgtct ccccgtccgg gtcgtagccc ggtggtgtgt
      781 agacgtacag ggggcgctcg tgcgggtcgc cgagccggtt gccgcgcagg gcctcgctcg
      841 tgatcgtgtg ctcgttgacg gtcccgcgga actcgatcga ccagggctcc atcgccacgc
      901 ctccctttcg ccttgggggt tcgctcgccc accggggcac ggttctcggc acacaggcga
      961 gcagtccccc aaaacctccc gctcggatct ccggattggg caagggccta tcatgcccga
     1021 tgtgggcgcc gtgtccgacg tcgtcgggat cggcctcgcg ggcggacagg gagtgcgcgc
     1081 gcgcccgctc acgctgaagg cgcccggcta cctgcgctcg aaggcggcga tggcgttcgt
     1141 cgggcggcgc ctgatccact gggtgatgca gatcctgacc gccgagggga tcaaggagta
     1201 ctacgtcgtc gcccacggca aggagaaccg ctaccagatc aaggtgctga tcggctacgg
     1261 cgagaacctc ggcgtcgacg tgcagtactc gccggtgaaa tacgacgtga tgagccgtgg
     1321 gagcgccgac tccacgctgc gccttctgga gcactgggac atcggccggc cggcgctcgt
     1381 gttcccgacc gactcgatca tcgacttcga cctggagccg atgctcctcc agcacctcga
     1441 gagcggcgcc gtggccacca tcgcggcgat gacccggccg ccggtcgagg tggccggcaa
     1501 gtacggcgtg atgctcaccg atccgcacgg gaagatcctc gagttcgtcg agaagccgac
     1561 gctcgaagag atccgggaac agtttcccgt caccgacgag cccgagttcg accgcctgcc
     1621 gctgctgacg aacgccgggt tctacctgct cgacgcggcc gccctgcgcc ggatcgccga
     1681 ggacccgagc ctcgcggccg tccgccgcaa ccgcctggac ttcgggatgg acctgctgcc
     1741 ctggctggta gggaacggtc acccggtcta cgcgcacgcc gtccggcgcc tcggcgacct
     1801 ggggaacatc gaggattacg tcgacaccac cgtcgacgtg ctgcacggcc gcttccgctc
     1861 catcgaccgg ctgatgggcg agcccttcga ccggtcgcgc aacgtctgga tcgcgcccga
     1921 gtcgctgcac atgcgcgacg cgctgaccgg caagtcgctc gccgagaagc tcgccgaggg
     1981 gctggtgacc atcggcccgg ccgtgcggat cggccgctac tgcgaggtcg gccccggggt
     2041 cgtgatcacc gagtcgaacc tggacgacga cgtggaggtc cgggagggcg cctacgtgtc
     2101 ccgtgcccag gtgcgcgacg gggcgatcat cggccaggcg gccaccgtca gcgaggcggt
     2161 ggtcgggtcg atgagcgagg ttcgctcgtc gcgcggcgac ccgacggtga t
//
LOCUS       DASPWO010000524         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_667371, whole genome shotgun sequence.
ACCESSION   DASPWO010000524 DASPWO010000000
VERSION     DASPWO010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667371"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VEO00_12025"
     CDS             <1..319
                     /locus_tag="VEO00_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58761.1"
                     /translation="AASTAGTAAGAADAQYASLVDRVRSTGLDAVVTVNSARDMGVGV
                     PEGALFDLELTLNLPGQPAVAVKQHAIVPPDDAPKLKPGVNLPAKATAGSPPQFVILW
                     DRV"
     gene            381..1382
                     /locus_tag="VEO00_12030"
     CDS             381..1382
                     /locus_tag="VEO00_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HYU58762.1"
                     /translation="MSTIRERWEDTERRILSTRATLAADSKGREREEEPHPLRTAFQR
                     DRDRIIHSKAFRRLKHKTQVFIAPEGDHYRVRLTHTLEVAQIARTLARALRLNEDLTE
                     AIALGHDLGHTPFGHLGEEAITPFLGRPFRHNEQSLRVVDHLENDGDGLNLTWEVRDG
                     IVNHTWSMPVPGTLEGQVARLADRAAYVNHDVDDAIRAGILTEADLPAEVHAVLGETH
                     GARINTLVGDAVASSEDRPDVALSEEVGNALDALRDFMFERVYLRAEAHEEQEKAISL
                     VRALFSYYVRHPDQIPPEYGRAPGDTATRVADYIAGMTDRYALRDYERIFLPQRWLL"
     gene            1386..>2463
                     /gene="dnaG"
                     /locus_tag="VEO00_12035"
     CDS             1386..>2463
                     /gene="dnaG"
                     /locus_tag="VEO00_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HYU58763.1"
                     /translation="MSGRIRQEDVETVRERTDIVQVVGQYLQLRKAGADRFVGLCPFH
                     TEKTASFGVSPSKQLYHCFGCGVGGNVFHFVMAIENLGFPEAVERLAKQAGITLRYEG
                     LSESDRRDLSRRGALHRANQLAAELYHANLMESPEAGAARDFLASRGLAREAAVEFGI
                     GLAPGLPDFLLRRLAGKLSAEILVEAGLVVKDARGALRDRFRGRVTFPLHDLTGRGVG
                     FGARLLAGEGPKYVNTPETAIYKKGELLYNLHRAKAAMARTGTAVVVEGYTDVIALAQ
                     GGVGTAVATCGTALSESHFRLLSRFAERAVLAFDSDEAGAKAAERAFAFHEQHPVEAV
                     VLVLPEGLDPADFVLKHGAGAFEEL"
BASE COUNT          359 a          897 c          883 g          324 t
ORIGIN      
        1 cgcggcttcc acggccggca ccgcggcggg ggcggccgac gcgcagtacg cctcgctggt
       61 cgaccgggtg cggagcaccg ggctcgacgc ggtcgtcacc gtgaactcgg cccgggacat
      121 gggtgtcggc gttcccgagg gagcgctgtt cgacctggag ctgacgctga acctgcccgg
      181 ccagccggcg gtcgcggtga agcagcacgc catcgtcccg ccggacgacg ccccgaagct
      241 gaagcccggc gtcaacctgc cggcgaaggc cacggccggc tcgccgccgc agttcgtcat
      301 cctctgggac cgggtctagt cagacgccgt ccagtcagag gccggcgggg gcgtcggaac
      361 cccgtgctac cctggcccgc gtgagcacga tccgcgagcg ctgggaagac accgagcgcc
      421 ggatcctctc cacgcgagcc acgctcgccg ccgactccaa gggccgggag cgcgaggagg
      481 aaccgcaccc gttgcgcacc gcgttccagc gcgaccgcga ccggatcatc cattcgaagg
      541 ccttccgccg cctgaagcac aagacgcagg tcttcatcgc cccggagggc gaccactacc
      601 gcgtccgcct gacccacacc ctggaggtgg cccagatcgc gcggaccctc gcccgggccc
      661 tgcgcctgaa cgaggacctc accgaggcga tcgccctcgg gcacgacctg ggccacacgc
      721 cgttcggaca cctcggcgag gaggccatca cgccgttcct cggccgcccg ttccgccaca
      781 acgagcagag cctgcgcgtc gtcgaccacc tggagaacga cggggacggg ctgaacctca
      841 cctgggaggt ccgcgacggc atcgtcaacc acacctggtc gatgccggtg cccggcaccc
      901 tcgaggggca ggtggcccgc ctggccgacc gcgccgccta cgtcaaccac gacgtcgacg
      961 acgccatccg cgccggcatc ctgaccgagg cggacctgcc ggccgaggtg cacgccgtcc
     1021 tcggggagac ccacggcgcg cggatcaaca ccctggtcgg cgacgccgtg gcgagcagcg
     1081 aggaccgccc ggacgtggcg ctctccgagg aggtcgggaa cgccctcgac gcgctgcgcg
     1141 acttcatgtt cgagcgggtc taccttcggg ccgaggcgca cgaggagcag gagaaggcga
     1201 tcagtctggt ccgggcgctg ttctcgtact acgttcgcca cccggaccag atcccgccgg
     1261 agtacgggcg ggcgccgggc gacacggcga cgcgggtggc cgactacatc gccgggatga
     1321 ccgaccgcta cgcgctgcgg gactacgagc ggatcttcct tccccagagg tggttgctct
     1381 aggggatgtc gggcaggatc cggcaggaag acgtcgagac ggtccgcgag cggaccgaca
     1441 tcgtgcaggt cgtcggccag tacctgcagc tgcgcaaggc cggcgccgac cgcttcgtcg
     1501 gcctgtgccc gttccacacg gagaagacgg cctcgttcgg cgtcagtccc tcgaagcagc
     1561 tctaccactg cttcggctgc ggcgtcggcg ggaacgtctt ccacttcgtg atggccatcg
     1621 agaacctcgg cttcccggag gccgtcgagc gcctggccaa gcaggccggg atcacgctgc
     1681 gctacgaggg gctgtccgag tccgatcgcc gtgacctgtc ccggcgcggc gcattgcacc
     1741 gggccaacca gctcgccgcc gagctgtacc acgccaacct gatggagtcg ccggaggcgg
     1801 gggcggcccg cgacttcctg gcctcccgcg ggctggcgcg cgaggcggcc gtggagttcg
     1861 ggatcggcct ggcgcccggc ttgcccgact tcctgctgcg gcgcttggcc gggaagctgt
     1921 cggccgagat cctggtcgag gccggcctgg tggtgaagga cgcccgcggc gcgctgcgcg
     1981 accggttccg cggacgggtg acgttcccgc tgcacgacct gaccgggcgg ggcgtcggct
     2041 tcggcgcgcg cctgctggcg ggggagggcc cgaagtacgt caacaccccc gagaccgcga
     2101 tctacaagaa gggcgaactg ctctacaacc tgcaccgggc gaaggcggcc atggcccgca
     2161 ccgggacggc cgtcgtggtc gagggctaca ccgacgtcat cgcgctggcg cagggcggcg
     2221 tgggaacggc cgtggccacc tgcggcaccg ccctgtccga gagccacttc cggttgctgt
     2281 cgcgcttcgc cgagcgcgcc gtcctggcct tcgactccga cgaggcgggg gcgaaggccg
     2341 ccgagcgcgc cttcgccttc cacgagcagc acccggtgga ggccgtcgtc ctggtgctgc
     2401 cggagggtct cgacccggcg gacttcgtgc tgaagcacgg cgccggggcg ttcgaggagc
     2461 tcg
//
LOCUS       DASPWO010000525         3127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_415665, whole genome shotgun sequence.
ACCESSION   DASPWO010000525 DASPWO010000000
VERSION     DASPWO010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415665"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /gene="ssrA"
                     /locus_tag="VEO00_12040"
     tmRNA           <1..297
                     /gene="ssrA"
                     /locus_tag="VEO00_12040"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            713..988
                     /locus_tag="VEO00_12045"
     CDS             713..988
                     /locus_tag="VEO00_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HYU58764.1"
                     /translation="MSETLPLAEIKAHLSEIVDRVEGHHERVTLTRNGRPAAVLISPE
                     DLEALEDTLELLADPGARSEIERARRDIARGKGIRADELRARYLDRA"
     gene            985..1260
                     /locus_tag="VEO00_12050"
     CDS             985..1260
                     /locus_tag="VEO00_12050"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYU58765.1"
                     /translation="MTRSSWELIVAPSAERSLARLPEKVAGAVVEFMLGQLLENPNRV
                     GHRLRGDLEGLWAARRGPYRVVYEIDERAGEIRMLRIDHRADIYRPR"
     gene            complement(1410..1757)
                     /locus_tag="VEO00_12055"
     CDS             complement(1410..1757)
                     /locus_tag="VEO00_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58766.1"
                     /translation="MTFETVPGFTDSPTAIFPIAINVSALVSGPPVEFRVLSTNVGGQ
                     THASKPGTTRFVPSGGSADSFGYLWVEQNQAAAPHSIFLRLQWRSPSGNTVHLLRGDM
                     SVEYTTDVCDGSS"
     gene            complement(2150..2581)
                     /locus_tag="VEO00_12060"
     CDS             complement(2150..2581)
                     /locus_tag="VEO00_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58767.1"
                     /translation="MELPRDSLRRLLGLATVALVVVAALVLPPSRQASAADDDCTLTV
                     SGPFLYAGMVSPVIEVRCDSVKQSIRIDAALDSDGSQVATSSRTCRRASSCVTGLADD
                     GIFALDVPGDQRWCGRGSAAIHDRGATYVLPEATSCETESF"
     gene            complement(2829..>3127)
                     /locus_tag="VEO00_12065"
     CDS             complement(2829..>3127)
                     /locus_tag="VEO00_12065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU58768.1"
                     /translation="DNAVRHAREGGSVRVRASRGDGSLTIEVADDGRGFDPAFLPKAF
                     DPFTRSEDGRGRDGGGAGLGLAIVKAVAEAHGGSAHADGAPDGGARVTIALPAT"
BASE COUNT          503 a         1092 c         1045 g          487 t
ORIGIN      
        1 tcgccacatg cgtcgtgaaa caggtggcac aaccagaagc gccgataacc acgcgctcgc
       61 tgcctaaaca ctaggcagcc gtcccccgcg gggtgcctgt ccccgcggac cgggcgtcat
      121 caacaggctc accggagggg gcatggcccc gtggtcctcc cgggacatcg cagcggggct
      181 gggtcggtgg tcggccgtcg gcggcgctcc accgaccgag aaccttcgcc gactacgctc
      241 gtagaagacc cggggagtcg cccgaggaca ggggttcgaa tcccctcacc tccacttgaa
      301 ccgtccggtg cgcgaaccac ggggggaacc cggtacgtcg gcaggatctc acgccggtcg
      361 atcacccgca attccttgat cagaagccgc aggagggcct tccgctgttg cgccgtgccg
      421 gctcgaagaa tcgcgccgaa gtctttggcc cattccgcgg cggccgcggc gctaaccgcc
      481 tctgacgggt cctcttcggc gccccctgtg ccaggaggcc tcctccgctt gcagcgcgtc
      541 gatcctcgtc ttcaacgacg cgatgcgctc gcggcaatcc gccggggaga gagacccctc
      601 ttcgaagagg ccccgaagta gcggtatcgt ctgaagctgg tgccgccggt cgccgttccc
      661 cttcccgaca gtttattgac gccggccgtt accgatgact agaatcctgg tcatgagcga
      721 gactctgccc cttgcggaga tcaaggccca cctatccgag atcgtagaca gggtcgaagg
      781 acatcacgaa cgggttaccc tcacgcgcaa cggccggcct gcggccgtgc tgatcagccc
      841 ggaggatctg gaggcgctgg aggacacgct ggaactgctg gccgatccgg gggcccgatc
      901 cgagatcgag cgggctcgtc gagacatcgc caggggcaag ggaatccgcg ccgacgagct
      961 gagggctcgc tacctggata gggcgtgacc cgctcctcgt gggagcttat cgtcgctccg
     1021 tcggcagagc ggagcttggc tcgattgccg gagaaagtgg ctggcgccgt cgtcgagttc
     1081 atgttgggcc agctcctaga gaatccaaac cgcgtggggc acagactgcg tggggacctt
     1141 gaaggcctct gggccgctcg tcgaggaccg taccgggtcg tctacgagat cgacgagcgc
     1201 gcaggggaga ttcggatgct gaggatcgat catcgggcgg acatctatcg acctcgatag
     1261 gtgaccttca tcgagccgtt gctcgcccac tggccagagg ggccggcgca aatgagcacc
     1321 tctgggcggt tcgcgcaccg cgcaatccac accacgttca acggcttgcc gaagccattt
     1381 cctgtcgttg acccgggggc gacctccgcc tacgacgagc cgtcgcacac atccgtggtg
     1441 tactcgaccg acatgtcgcc ccgcagcagg tgcaccgtgt tgccgcttgg actccgccat
     1501 tggagccgga ggaagatgct gtgcggggct gctgcctggt tctgctcgac ccacaggtat
     1561 ccgaacgagt ccgcgctgcc cccgctaggc acgaaccgcg tcgtcccggg cttgctggcg
     1621 tgggtctgcc ctcccacgtt ggtactcagg acgcggaact ccaccggcgg tccggaaacg
     1681 agggcgctga cgttgatcgc gatcggaaag atggcagttg gagagtcggt gaaccccggg
     1741 accgtttcga aggtcatcga ggaagtggaa gcctcggtgg tccgccagac ggtgtccagg
     1801 cagctcaccg gggtgctgcc cctccccgta tcggccacga cggccgccca cgccgcccct
     1861 cccgagcgcc gctgcgttcg aattccttgc gacagtgtcc acagcagccg gagagtgggg
     1921 cacgctgggg tgtggcgtcg acgggacgtc cggacgttac tccgtgcgcc agcggacggc
     1981 ggagaccctg tggaatcccg cgaggccctt cagttccacc tccaccgggt cttcgaacga
     2041 gaacgctccc gaactctcga tgagctcgcg gacgagagag gagaccagga tctcccgccc
     2101 cgcgcctccg cagcgatgcg tgctgcgagg atgaccgccg tccaatcggt tagaagctct
     2161 ccgtttcgca cgaggttgcc tcggggagga cgtatgtcgc ccccctgtcg tggatcgcgg
     2221 cagacccccg gccgcaccag cgctggtcgc ccggaacgtc cagggcgaag atgccgtcgt
     2281 cggccagccc ggtcacgcag ctactggccc ggcgacaggt ccggctggaa gtcgccacct
     2341 gcgatccatc cgagtcgagc gcggcgtcga tacgaatgct ctgcttgacc gaatcgcaac
     2401 ggacctcgat gaccggggac accatgcccg cgtagaggaa gggccccgag accgtcagcg
     2461 tgcagtcgtc gtcggcggcg gacgcctgcc gcgacggcgg caggacgagg gccgcgacca
     2521 cgaccagcgc cacggtggca agtccgagca gcctcctcag agagtccctc ggcagctcca
     2581 tctctcccct ccccagagcc ctgtggctct tccagagagc gccttccggc tggtactacg
     2641 ggcgagcgag gtgtgaagtt ccctcctgtc gtgaggccac gccgccggaa gtgcctcacc
     2701 ccgtgcgggc tgcggagcgg tggcctggtg gacccgggct ccgccggcgc cgtccagcag
     2761 cgttccctgc gcaccccggc gagcactgcc gacacgcggt cctccatcgg ctcgacccgc
     2821 gacgacgctc aggtcgccgg cagggcgatc gtgacgcgag cgccgccatc aggcgccccg
     2881 tcggcgtggg cggacccccc gtgggcctcc gcgacggcct tgacgatggc caggccgagc
     2941 ccggcgccgc ccccatcacg gccgcggccg tcctcggacc tggtgaaggg gtcgaacgcc
     3001 ttcgggagga aggccgggtc gaacccccga ccgtcgtcgg ccacctcgat ggtcagcgag
     3061 ccgtcgcccc tcgacgccct cacccggacg ctgccgccct ctctggcgtg gcggacggcg
     3121 ttgtcga
//
LOCUS       DASPWO010000526         4319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_415666, whole genome shotgun sequence.
ACCESSION   DASPWO010000526 DASPWO010000000
VERSION     DASPWO010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415666"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VEO00_12070"
     CDS             complement(<1..1150)
                     /locus_tag="VEO00_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HYU58769.1"
                     /translation="MASRTSTQAGSGLDRYFKISERGSTVRTEVVAGLATWLTMAYIL
                     FVNPGILTSLPDHTGLTLPFNQVVTVTALVAGVMTILMGVYANYPFAMAAGLGLNAFV
                     AFTLVGANGLSWPEAMGVIVIEGLAITVLVLTGFRTAVLNAIPMDLKRAIGIGIGLFI
                     AFIGLRNAGIVVVGEGTPLRLAPDLTSWPIVVFAFGLAVTATLVARKMRGALLIGILA
                     TTILAIIVNAATDAPYWAPGVAELPDKVAETPNFGLVGDFSFNVFKVLGLASALAAIL
                     SVMLSDFFDTMGTVIGIGGEAKLLDSEGKLPGINRVLLVDSLGAAAGGAASASSNTTY
                     IESAAGVAEGGRTGLASVVTGALFLVALFLAPIAGVVPNEATAPVLVLV"
     gene            1281..1631
                     /locus_tag="VEO00_12075"
     CDS             1281..1631
                     /locus_tag="VEO00_12075"
                     /inference="COORDINATES: protein motif:HMM:NF039182.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HYU58770.1"
                     /translation="MTEDTPMDPEAPREEPGPVEAVVKKASRFSREFLATVVALVSTA
                     FGVVAALAWNSAIQELFTALDISTANKVTALFIYAVIVTVIGVVVIVYLGRLATRIGA
                     EPVEFKFPVPKKSE"
     gene            1636..2448
                     /locus_tag="VEO00_12080"
     CDS             1636..2448
                     /locus_tag="VEO00_12080"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HYU58771.1"
                     /translation="MAKKRKKRPRPPGQAGPATAGAATDTAAPPRGETRPQPRPQPTR
                     RHKEEARRAKELALKRLRRRTLLRRLGTFAVMAAVIGGVTTFFLVRNAATTRTVTAAN
                     RAAEAAGCTAPGDIERPLDPAYLAGNNVANHLGPGETATYNTVPATSGRHTSSTLPAD
                     RHVYTEAVPEQTAVHNLEHGYVLIYYRADGDGALAKGVVDRLASFARGEGKVILAPYE
                     SLEVGTALAFVAWQDLRTCPAGVTPAQAADMAEGFVADYRSGGGNAPEPNGV"
     gene            complement(2445..>4319)
                     /locus_tag="VEO00_12085"
     CDS             complement(2445..>4319)
                     /locus_tag="VEO00_12085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HYU58772.1"
                     /translation="PRATRAPRGVPRTVMRTIRSLAVALAVAAAMLAPASAARAGGGD
                     FTFYGAGWGHAVGMGQYGAYGLANDGWSTNRIVTHFYRGTKVAATTVPGAVRVGLLQG
                     SRSIGIKAVDAGVRLNLNSGSGPAVATVPVGQSWTVRLRYGVYWVQKSDGTYVGNHGF
                     GTSDSKLVASYRPTGSAYLPSEGHTYGWGTLEFNIYRPCDDCIQTMRALNVLGMQAYL
                     YGIAEVPSSWPTAALEAQAIAARTYALARIAAYGQNRPTCNCALYDSTYDQVFVGSDK
                     ERGVDGDRWVDAVGNTLDKIAMAGTAAAGGYYHSSSGGYTENNEVTLGGAPASYLRGV
                     CDPGDYTTSNPNRFWTVSFTAAYIADRIALYTGVDVGTVTGISISDRGVSGRVISANV
                     TGTAGSTTVSGSTLKSALNLKDTRVWVNSDRIVTGEIRTRYDQLMCAPGLQLSASFEV
                     DGGRYQRFDDGRIYANYDRGVAYWVHDGVLEKYLDLGAHDSFLGLPETNVLYDDSANG
                     YWTTFEGGRIYWSARTGGHELHGPVYERYKAEGAHDSGLGLPTSDVVKSGGTESAEFE
                     HGTITCNLSNDTCTVNGAGARRSSAVPVPGPAATPSVSPTPSPSASPSVSPSATSSPG
                     "
BASE COUNT          660 a         1542 c         1522 g          595 t
ORIGIN      
        1 cgaccaggac cagaaccggc gcggtggcct cgttcggcac caccccggcg atcggggcga
       61 ggaacagggc caccaggaac agcgcgccgg tgaccaccga cgccagaccg gtgcgccccc
      121 cctcggccac gccggccgcg ctctcgatgt aggtcgtgtt cgacgacgcc gacgccgcgc
      181 ccccggcggc cgcgccgagc gagtcgacca gcagcacccg attgatcccc ggcagcttcc
      241 cctcgctgtc gagcagcttc gcctcgccgc cgatcccgat cacggtgccc atcgtgtcga
      301 agaagtccga cagcatcacc gagaggatcg cggcgagcgc cgaggccaac ccgagcacct
      361 tgaagacgtt gaacgagaag tcgccgacga gtccgaagtt cggcgtctcg gcgaccttgt
      421 ccggcagttc cgcgacgccg ggagcccagt agggggcgtc ggtggccgcg ttgacgatga
      481 tcgccaggat ggtcgtggcc aggatcccga tcaggagcgc gccgcgcatc ttccgggcca
      541 cgagggtcgc ggtgacggcg agcccgaacg cgaacaccac gatcggccag ctggtcaggt
      601 ccggcgccag gcggagcggt gtgccctctc cgaccaccac gatccccgcg ttgcgcagcc
      661 cgatgaaggc gatgaacagg ccgatcccga tcccgatggc ccgcttcagg tccatcggga
      721 tcgcgttgag caccgcggtg cggaagccgg tcaggacgag caccgtgatc gccaggccct
      781 cgatgacgat cacccccatc gcctccggcc aggacaggcc gttcgctccg acgagggtga
      841 acgcgacgaa cgcgttcagt cccagcccgg cggccatggc gaacgggtag ttcgcgtaga
      901 cccccatcag gatcgtcatc accccggcga cgagcgcggt gacggtgacc acctggttga
      961 acggaagcgt caggccggtg tggtcgggaa gcgacgtcag gatccccgga ttgacgaaca
     1021 ggatgtaggc catggtcagc caggtggcca acccggcgac cacctccgtt cgcacggtgg
     1081 acccgcgctc ggagatcttg aagtaacgat ccaggccgga gccggcctgg gtggacgtcc
     1141 tcgacgccat cgtccccctc ccccctttcg acctgccgcg gggaaggcgg aacaagcata
     1201 cggagccggt caagccggag caccgactag catcggaacc tcgcccacgc ccccgcgccc
     1261 cggaagagag gaggaacctt ttgaccgagg acacccccat ggacccggag gctccgaggg
     1321 aagagcccgg tcccgtcgag gcggtcgtga agaaggcgag ccgattctcc cgcgagttcc
     1381 ttgccaccgt cgtcgcgctc gtctcgacgg cgttcggcgt ggtcgccgcg ctcgcctgga
     1441 acagcgcgat ccaggagctg ttcacggcgc tggacatctc caccgcgaac aaggtcaccg
     1501 ccctgttcat ctacgcggtg atcgtcaccg tgatcggggt cgtggtgatc gtctacctgg
     1561 ggcgcctcgc gacccggatc ggagccgagc cggtagagtt caagttcccc gtcccgaaga
     1621 agagcgagta gtcgcgtggc gaagaagcgg aagaagcggc cccggccgcc ggggcaggca
     1681 ggcccggcca ccgccggcgc ggcgaccgac accgcggccc cgccgcgggg agagacgcgg
     1741 ccgcagccgc gcccccagcc gacccggcgg cacaaggagg aggcgcgccg ggcgaaggag
     1801 cttgccctga agcggctccg gcgccggacc ttgctccgcc ggctggggac gttcgcggtg
     1861 atggccgcgg tgatcggcgg ggtgacgacc ttcttcctgg ttcgcaacgc ggccacgacc
     1921 cggacggtca ccgcggccaa ccgcgcagcg gaggcggcgg gctgcaccgc gcccggcgac
     1981 atcgagcggc cgctcgatcc cgcgtacctc gccggcaaca acgtcgccaa ccatctcggc
     2041 ccgggcgaga cggccacgta caacaccgtt ccggccacct ccggccggca cacctcgtcg
     2101 acgctccccg cggatcgcca cgtgtacacg gaggccgtgc ccgagcagac ggccgtccac
     2161 aacctcgagc acggctacgt cctgatctac taccgggccg acggcgacgg cgcgctggcg
     2221 aagggcgtgg tcgaccgtct ggcctcgttc gcccgcgggg agggcaaggt catcctggcg
     2281 ccgtacgaga gcctcgaggt cggcaccgcg ctcgccttcg tcgcctggca ggacctgcga
     2341 acctgcccgg ccggcgttac cccggcccag gcggcggaca tggccgaggg gttcgtcgcc
     2401 gactaccgca gcggcggcgg gaacgccccc gagccgaacg gcgtctagcc cggcgaggac
     2461 gtcgcgctcg gcgagaccga cggcgaggcg ctcggcgacg gtgtcggcga gaccgacggg
     2521 gtggcggccg ggccggggac cgggacggcg gaggaccgcc gggcacccgc cccgttcacg
     2581 gtgcaggtgt cgttcgagag gttgcaggtg atcgtgccgt gctcgaactc ggccgactcg
     2641 gtcccgccgc tcttcacgac gtcgctcgtc gggaggccga gtcccgagtc gtgggccccc
     2701 tcggctttgt agcgctcgta gaccgggccg tgcagctcgt ggccgcccgt ccgcgcggac
     2761 cagtagatcc gtccgccctc gaacgtcgtc cagtagccgt tcgccgagtc gtcgtacagc
     2821 acgttcgtct ccggcaggcc caggaagctg tcgtgcgccc cgaggtcgag gtacttctcg
     2881 agcacgccgt cgtgcaccca gtacgccaca ccgcggtcgt agttcgcgta gatccggccg
     2941 tcgtcgaagc gctggtagcg gccgccgtcg acctcgaacg aggccgagag ctgcaggccg
     3001 ggcgcgcaca tcagctggtc gtagcgggtc cggatctctc cggtcacgat ccggtcggag
     3061 ttcacccaga cccgggtgtc cttcaggttg agcgccgact tcagcgtgga tcccgagacc
     3121 gtcgtcgatc ccgcggttcc ggtgacgttg gcggagatca cgcggcccga cacgccgcgg
     3181 tcggagatcg agatcccggt gacggtgccg acgtcgacgc ccgtgtacag cgcgatgcgg
     3241 tcggcgatgt acgcggccgt gaagctcacc gtccagaacc ggttcgggtt gctcgtcgtg
     3301 tagtcgccgg ggtcgcacac gccgcgcagg taggaggccg gcgcgccgcc caacgtgacc
     3361 tcgttgttct ccgtgtagcc gcccgaggac gagtggtagt agccgccggc cgcggcggtg
     3421 ccggccatgg cgatcttgtc gagcgtgttc ccgacggcgt ccacccagcg gtcgccgtcc
     3481 acgccgcgct ccttgtccga tccgacgaac acctggtcgt acgtgctgtc gtacagcgcg
     3541 cagttgcacg tcggccggtt ctgcccgtac gcggcgatgc gggccagggc gtaggtacgc
     3601 gccgcgatgg cctgcgcttc gagcgcggcc gtcggccagg acgatggcac ctcggcgatg
     3661 ccgtacaggt aggcctgcat cccgagcacg ttcagcgcgc gcatcgtctg gatgcagtcg
     3721 tcgcacggcc ggtagatgtt gaactcgagg gtcccccagc cgtacgtgtg gccctcggac
     3781 gggaggtacg ccgagccggt cggacggtac gaggcgacga gcttggagtc cgaggtcccg
     3841 aacccgtggt tgccgacgta ggtcccgtcg gacttctgca cccagtagac gccgtagcgc
     3901 agccgtaccg tccagctctg gcccaccggg acggtggcga cggccgggcc ggagcccgag
     3961 ttcaggttca ggcggacccc cgcgtccacc gccttgatcc cgatcgagcg cgagccctgc
     4021 aacaggccga cccgcacggc gccggggacg gtcgtcgcgg cgaccttcgt tccccggtag
     4081 aagtgcgtca cgatccggtt ggtgctccaa ccgtcgttcg ccaggccgta cgcgccgtac
     4141 tggcccatcc cgacggcgtg cccccacccg gcgccgtaga acgtgaagtc gccgccgccg
     4201 gcgcgagcgg cggaggccgg cgcgagcatc gcggcggcga cggccagggc gacggcgagg
     4261 gatcggatcg ttcgcatcac ggttcggggg acgcctctcg gggcgcgtgt cgctctcgg
//
LOCUS       DASPWO010000527         4017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_415908, whole genome shotgun sequence.
ACCESSION   DASPWO010000527 DASPWO010000000
VERSION     DASPWO010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415908"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VEO00_12090"
     CDS             complement(<1..587)
                     /locus_tag="VEO00_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58773.1"
                     /translation="MSKRAAAALLAFTPAVVLIAAPTRPASAAPALPPLSHVAVVVMD
                     RVTIDQVAAMPAVRDAPDGWTAGLVSTAGRGVVGPTAGALTFSAGARAIPPAGEDVPF
                     TLAPGPVDERLPTVGTVGDAYTARTGEREPAATDPEMPPFVYPDIDLVAQLNARPEVR
                     ARPGLLGSEFADRNIDRAVVTIREAPVRLAPLVAM"
     gene            615..1997
                     /locus_tag="VEO00_12095"
     CDS             615..1997
                     /locus_tag="VEO00_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HYU58774.1"
                     /translation="MLDPELLSEVLAAALSTGGRFAEVFVEDRVSTSIRLDDGRVEEL
                     TSGLDRGAGIRVLLGESQAYAHTNRWDRDALVEAARAAAAAATAEGGTTMVPFRHAET
                     VPVHDVRIPFADVPAERKVDWVREADDAARAHSSDVRQVIAGYGESEQRVLIANSEGL
                     WAEDVRPRIRVTVHVVAARDGVIQTGFHSPGGLTGAEFLEEHTGRAVAEEAARQAVTM
                     LDGRPAPAGEMAVVMGPGGGGVMFHEACGHGLEADLIQKEASLYRGKLGQRLASPLFT
                     GVEDATLRSAWGSYAFDDEGTPSSRVVLFEKGVLTDYLYDRVRAERDGVASNGHGRRQ
                     SYAHLPIPRMANSHILGGDDDPEAIVAGTARGLYARKLGGGQVNPATGDFVFGVAEGY
                     LIEHGELAYPVRGANLIGNGPAVLSAIDALAADFDVFHGVCGKDGQGVPVSNGSPTLR
                     IGRMTVGGTG"
     gene            1997..3334
                     /locus_tag="VEO00_12100"
     CDS             1997..3334
                     /locus_tag="VEO00_12100"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HYU58775.1"
                     /translation="MAETDELVRRAVEAAAGGESLEGFAVESRRTQVKAHGGTVESLS
                     FAESRGLGVRVIVDGRLGYAYATDPDLEEVAGLVARARENAGLGTPDEANRLPEAVPF
                     RPLDGIYRPALAAHPTEGKVALALDLERVATSALPQVRKLEEAAYGDAVSRVAIASTA
                     GVAAEYARTDCWCSAFAIAEDGEDTQTGFDFRLGHELDDLDWETVARDAAERAARLLG
                     AAKPGTERLPVLLDPLAAASFLGVLAGALSAESVQKGRSLFAGLVGEAVASAAVTLVD
                     DGRFLDGPAASPFDDEGVPTGRTDLIGSGRLLEFLHNTVTARRGNTVSTGNASRGSYR
                     TVPGVAPSNLFLEPGPDEPAALLARAGRAIYVQDVTGVHSGANPVSGEFSVGATGLRV
                     ADGTLAEPLREMTIASTLIEMLRAVEAVGADLRFGGAVGSPTVLVGEMTVAGT"
     gene            complement(3357..>4017)
                     /locus_tag="VEO00_12105"
     CDS             complement(3357..>4017)
                     /locus_tag="VEO00_12105"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HYU58776.1"
                     /translation="VAVVAYFWRDLWGILRGSLRALAGRGDERDRRRARLLGLLAIGS
                     VPAAVLGVAFGDSVEETFQEPQVVALLLLVTAGLLVAADTLHARRAHHREVDSIGLTD
                     AVVIGLFQGIALLPGISRSGSTIAAGVGRGLSREAAARFSFLLSIPAIVGAALVKVPD
                     LSGSANLGTVALGTLAAAAVGFASIWFLLRYLRHGRLVPFAVYCVVAAAIGLVFWLQY
                     K"
BASE COUNT          538 a         1454 c         1547 g          478 t
ORIGIN      
        1 tccatggcca cgaggggcgc gaggcggacc ggcgcctccc ggatcgtcac gacggcccgg
       61 tcgatgttcc ggtcggcgaa ctccgacccg agcaacccgg gccgggcccg gacctcgggg
      121 cgggcattga gctgcgcgac caggtcgatg tccgggtaga cgaacggtgg catctccggg
      181 tccgtcgcgg ccggctcgcg ctccccggtc cgggccgtgt acgcgtcgcc cacggtgccg
      241 accgtcggca gccgctcgtc caccggcccg ggcgcaagcg tgaacggcac gtcctcgccg
      301 gcgggaggga tcgcccgggc tccggcggag aaggtcagcg cgccggcggt gggcccgacg
      361 acaccccgcc cggcggtgga gacgagcccg gccgtccatc cgtccggcgc gtcgcggacc
      421 gccggcatcg ccgcgacctg gtcgatcgtc acgcggtcca tgaccacgac ggcgacgtgg
      481 gagagcgggg ggagcgccgg cgcggcggac gcgggtcggg tgggcgccgc gatgaggacg
      541 accgccggcg tgaaggcgag cagggccgcc gcggcgcgct tggacaaacc ggcccgagtg
      601 tacgatgcgg cctcgtgctc gaccccgagc tcctctccga ggtcctggcc gccgccctct
      661 cgaccggcgg gcgcttcgcc gaggtgttcg tcgaggaccg cgtctcgacc tcgatccgtc
      721 tggacgacgg ccgcgtcgag gagctgacct ccgggctcga ccggggcgcc gggatccgcg
      781 tcctgctcgg cgagtcgcag gcctacgcgc acaccaaccg ctgggatcgc gacgcgctgg
      841 tcgaggcggc ccgcgcggcg gccgccgcgg cgaccgccga gggcggcacg acgatggtgc
      901 cgttccggca cgccgagacc gttcccgtgc acgacgtgcg gatccccttc gccgacgttc
      961 ctgccgagcg caaggtcgac tgggtccggg aggccgacga cgcggcccgg gcccactcga
     1021 gcgacgtgcg acaggtgatc gccggctacg gcgagtcgga gcagcgcgtg ctgatcgcga
     1081 actccgaggg gctgtgggcc gaggacgtcc ggccccggat ccgcgtcacg gtccacgtgg
     1141 tcgcggcgcg cgacggggtg atccagaccg ggttccacag cccgggcgga ctgaccgggg
     1201 cggagttcct cgaggagcat accgggcggg cggtggccga ggaggccgcg cgccaggcgg
     1261 tgacgatgct cgacggccgc ccggcgccgg ccggcgagat ggcggtggtg atggggccgg
     1321 gtggaggggg cgtgatgttc cacgaggcct gcggccacgg cctggaggcc gacctgatcc
     1381 agaaggaagc gtcgctgtac cgcggcaagc tcgggcagag gctcgcctcg ccgctcttca
     1441 ccggcgtgga ggacgccacg ctgcgcagcg cctgggggtc ctacgcgttc gacgacgagg
     1501 gcacgccgtc gtcgcgggtg gtgctgttcg agaagggcgt gctgaccgac tacctgtacg
     1561 accgtgtccg cgccgagcgc gacggcgtgg cctcgaacgg ccacggccgg cgccagtcct
     1621 acgcccacct gccgatcccg cggatggcga actcgcacat cctcggcggc gacgacgacc
     1681 ccgaggccat cgtcgccgga accgcgcgcg gcctgtatgc gcgcaagctg ggcggcggcc
     1741 aagtcaatcc ggccaccggc gacttcgtgt ttggcgtggc cgagggctac ctgatcgagc
     1801 acggcgagct cgcgtacccg gtgcgcggcg cgaacctgat cgggaacggc ccggcggtcc
     1861 tgtcggccat cgacgcgctg gccgccgact tcgacgtctt ccacggggtg tgcgggaagg
     1921 acgggcaggg cgtgccggtc tcgaacggca gccccacgtt gcggatcggg cggatgacgg
     1981 tcggcgggac cggctgatgg ccgagaccga cgagctggtc cggcgcgcgg tcgaggcggc
     2041 cgccggcggc gagtcgctgg aggggttcgc cgtcgagagt cgccggacac aggtcaaggc
     2101 gcacggcggg acggtcgagt cgctgtcgtt cgccgagagc cgcggcctcg gcgtgcgcgt
     2161 gatcgtcgac gggcggctcg ggtacgcgta cgccaccgac cccgatctcg aggaggtcgc
     2221 gggcctcgtc gcccgggcgc gcgagaacgc cggcctggga acgcccgacg aggcgaaccg
     2281 cctgcccgag gcggtgccgt tccggccgct cgacgggatc taccggccgg cgcttgccgc
     2341 gcaccccacc gagggcaagg tggcgctggc gctcgacctg gagcgcgtgg ccacctcggc
     2401 cctgccgcag gtgcgcaagc tggaggaggc ggcgtacggc gacgccgtct cgcgcgtggc
     2461 gatcgcctcc accgccggcg tcgccgcgga gtacgcccgg accgactgct ggtgctcggc
     2521 cttcgccatc gccgaggacg gcgaggacac gcagaccggc ttcgacttcc gtctcggcca
     2581 cgagctggac gacctggact gggagaccgt ggcccgcgac gcggccgagc gcgccgcgcg
     2641 cctgctcgga gcggcgaagc cgggcaccga gcggctgccg gtcctcctcg accccctggc
     2701 ggcggcgtcg ttcctcggcg tgctcgccgg cgcgctctcc gccgagtccg tgcagaaggg
     2761 gcgctcgctc ttcgccgggc tggtcggtga ggcggtggcc tcagcggcgg tgacgctcgt
     2821 ggacgacggg cgcttcctgg acggtcccgc cgcgtccccg ttcgacgacg agggcgttcc
     2881 gaccgggcgt accgacctga tcggttccgg ccggctcctg gagttcctgc acaacacggt
     2941 cacggcccgc cgcgggaaca ccgtctcgac cgggaacgcc tcgcgcgggt cgtaccgcac
     3001 cgtccccggc gtcgcgccgt cgaacctgtt cctcgagccg ggcccggacg agccggcggc
     3061 gctgctggcc cgggccgggc gggcgatcta cgtccaggac gtcaccggcg tgcactctgg
     3121 cgcgaacccg gtcagcgggg agttctccgt cggcgccacc ggcctgcgcg tggcggacgg
     3181 cacgctggcc gagccgctgc gcgagatgac gatcgcctcg acgctgatcg agatgctgag
     3241 ggcggtcgag gcggtcgggg ccgacctgcg cttcggcggc gccgtcggct cgcccacggt
     3301 gctcgtgggc gagatgacgg tcgccgggac gtagtccgaa gagcacgggc cgtggactac
     3361 ttgtactgca gccagaagac gagcccgatc gccgccgcga ccacgcagta gaccgcgaac
     3421 ggcacgagcc ggccgtgccg caggtagcgc aacaggaacc agatcgaggc gaacccgacc
     3481 gcggcggcgg ccagcgtccc cagggccacc gtccccaggt tcgcgctacc cgagaggtcg
     3541 gggaccttga ccagcgcggc gccgacgatc gccgggatcg acaggaggaa cgagaacctc
     3601 gccgccgcct cccgggacag gccccggccg acgccggcgg cgatcgtcga acccgaccgc
     3661 gagattcccg ggagcagcgc gatgccctgg aacagcccga tgacgacggc gtccgtgagc
     3721 ccgatcgagt cgacctcgcg gtggtgcgcg cgccgcgcgt gcagcgtgtc ggcggcgacg
     3781 agtaggcccg cggtgacgag cagcagaagg gccaccacct gcggttcctg gaaggtctcc
     3841 tccaccgaat ctccgaacgc aacgcccagg acggccgccg gtaccgaccc gatcgccagc
     3901 aggccgagca ggcgggcgcg tcgccggtcc cgctcgtcgc cgcgaccggc cagcgcccgc
     3961 agcgagccgc gcaggatccc ccacaggtcg cgccagaaat aggcgacgac cgcgacg
//
LOCUS       DASPWO010000528         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_754508, whole genome shotgun sequence.
ACCESSION   DASPWO010000528 DASPWO010000000
VERSION     DASPWO010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754508"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VEO00_12110"
     CDS             complement(<1..541)
                     /locus_tag="VEO00_12110"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF014870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HYU58777.1"
                     /translation="MSGRPRDLETLLHRAGRTAARAEDEAAVAAVARFAASAADEADV
                     AYAFVDSPLGRLLVAGTRRGLNRVEYADEDGDALLDDIAATISPRILEAPARLDDIRR
                     QLDEYFEGRRHAFDVTLDLRPAEGFRRRVLRATWRIPYGSVATYRDVATRAGNAAAVR
                     AAGNAVGWNHIPIVIPCHRV"
     gene            complement(538..1002)
                     /locus_tag="VEO00_12115"
     CDS             complement(538..1002)
                     /locus_tag="VEO00_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYU58778.1"
                     /translation="MKGAPPFQRLLEEQGPVVHRYLRAVAGQEADDLFQETFLAALRA
                     YPRLADARNLRGWVLRIATRKAIDAGRRRSRQAIPVEEVPEVPAPPSPDGEPALWAAV
                     RSLPPKQRAAVVHRYVYDLTYEEIAGLMGGTAEAARANASQGVRRLREVWEG"
     gene            complement(1047..1814)
                     /locus_tag="VEO00_12120"
     CDS             complement(1047..1814)
                     /locus_tag="VEO00_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HYU58779.1"
                     /translation="MPGDERPPPERDGLSGIAPGERDRALALLRAGRMRLLGLIEGAS
                     NGTFLAEVADGHGSALAVYKPRRAEAPLWDFPDGTLYRREVAAFELADALGWPHVPPT
                     AVVDGSLGVGSAQLFVEHDPEEHFFALAGTRLDDFAEVALFDAIANNADRKAGHCLHG
                     PDGNVWLVDHGVCFAEEPKLRTVIWDFAGDPLPEALRERVAAAAASLDGGEAADRLSA
                     LLAPGEVRAAADRAARLAATGCFPLPGSSRSVPWPPV"
     gene            complement(1772..2278)
                     /locus_tag="VEO00_12125"
     CDS             complement(1772..2278)
                     /locus_tag="VEO00_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HYU58780.1"
                     /translation="MEFDPVERITADAVGVPGQRTFYLQARHGKRSVHVLVEKDQVRL
                     LAASILEILQRVGKETSVDAVPDEDLALVEPDPNAWRAGRLSIGYQEERDLLVLDVEE
                     LVPEEDERPPQELRIWATREQMLALARHGAAAVAAGRPACQFCGNPIDPEGHWCPGMN
                     GHRQSGMV"
     gene            complement(2309..>2745)
                     /locus_tag="VEO00_12130"
     CDS             complement(2309..>2745)
                     /locus_tag="VEO00_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYU58781.1"
                     /translation="LRPEPGLIEVGYGEWSGKTFRQLLRRRLWTAVRETPSRVRFPGG
                     ETFGEVQARAVAAVDRIVTAHRRGTVVACSHADVIRLLIAHYSGVHLDLFQRVIVSPA
                     SVTAVALGEHPPRLLRVNDTGTLADLVPSAPPPRTARGPGKR"
BASE COUNT          350 a         1058 c         1002 g          335 t
ORIGIN      
        1 ccacgcggtg gcacgggatg acgatcggga tgtggttcca gccgaccgcg ttgccggccg
       61 cgcgcaccgc tgcggcgttc ccggcgcggg tcgccacgtc gcggtaggtg gccaccgatc
      121 cgtaggggat gcgccacgtg gcccgcagga cgcgccggcg gaacccctcg gcggggcgca
      181 ggtcgagcgt gacgtcgaac gcgtggcggc gcccctcgaa gtactcgtcg agctggcgcc
      241 ggatgtcgtc caggcgggcc ggagcctcga ggatgcgcgg gctgatcgtg gcggcgatgt
      301 cgtcgagcag ggcgtccccg tcctcgtccg cgtactcgac ccggttcagg ccgcgccggg
      361 tgccggcgac gagcagccgc ccgagcggcg agtccacgaa ggcgtaggcc acgtcggcct
      421 cgtccgcggc ggaggccgcg aaccgcgcca ccgccgcgac ggccgcctcg tcctccgccc
      481 gggcggccgt ccgccccgcg cggtggagca gcgtctccag gtccctcggc cgtccgctca
      541 tccctcccac acctcccgca ggcggcgcac gccctgcgac gcgttggccc gcgccgcctc
      601 ggcggttccg cccatcagcc cggcgatctc ctcgtaggtc aggtcgtaca cgtagcggtg
      661 caccaccgcc gcgcgctgct tcggcgggag cgaccgcacc gcggcccaca gcgccggctc
      721 gccgtccggc gacggcggcg cggggacctc cggcacctcc tcgaccggga tcgcctggcg
      781 tgaccgtctc cggcccgcgt cgatcgcctt gcgggtggcg atgcgcagca cccacccgcg
      841 caggttgcgc gcgtcggcca gccgtgggta cgcccgcagc gccgcgagga acgtctcctg
      901 gaacaggtcg tcggcctcct gtccggccac cgcccgcagg taccggtgca ccaccgggcc
      961 ctgctcctcc agtaatcgct ggaacggcgg cgctcccttc acaacgggag aacgacgcgg
     1021 gcggcccggg cgtgagacgg ggccggtcac accggcggcc acgggacgct gcgggacgat
     1081 ccggggagtg ggaagcagcc cgtcgcggcg aggcgcgcgg cccgatccgc ggcggcgcgg
     1141 acctcacccg gcgccaggag cgcggagagc cgatccgccg cctccccccc gtcgagcgac
     1201 gcggccgccg cggccacgcg ctcccgcaac gcctccggca gcgggtcgcc ggcgaagtcc
     1261 cagatcaccg tgcgcagctt cggctcctcg gcgaagcaca cgccgtggtc cacgagccag
     1321 acgttcccgt ccgggccgtg caggcagtgc ccggccttgc ggtcggcgtt gttggcgatg
     1381 gcgtcgaaca gggcgacctc cgcgaagtcg tccaggcgcg tcccggccag cgcgaagaag
     1441 tgttcctcgg ggtcgtgctc gacgaagagc tgcgccgagc cgactccgag cgatccgtcg
     1501 accacggcgg tgggcggcac gtgcggccag ccgagcgcgt cggccagctc gaacgcggcc
     1561 acctcccgtc ggtagagcgt cccgtcgggg aagtcccaga gcggggcctc tgcccgtcgc
     1621 ggcttgtaca cggcgagcgc gcttccgtga ccgtcggcca cctcggccag gaaggtgccg
     1681 ttcgaggcgc cctcgatcag accgagcagg cgcatccggc ctgcgcgcag gagcgccagc
     1741 gcccggtcgc gctcgcccgg ggcgatcccg ctcagaccat cccgctctgg cggtggccgt
     1801 tcatccccgg gcaccagtgg ccctccggat cgatcgggtt gccgcagaac tggcaggccg
     1861 gccgcccggc ggcgacggcc gccgcgccgt gccgcgccag cgcgagcatc tgctcgcggg
     1921 tcgcccagat ccgcagctcc tgcggcgggc gctcgtcctc ctcgggcacc agctcctcga
     1981 cgtccagcac cagcaggtcg cgctcctcct ggtagccgat cgacaggcga ccggcccgcc
     2041 aggcgttggg gtccggctcg acgagggcca ggtcctcgtc ggggacggca tcgaccgacg
     2101 tctccttccc gacgcgctgc aggatctcga ggatcgaggc cgccagcagc cggacctggt
     2161 ccttctcgac caggacgtgc acggaccgct taccgtggcg cgcctgcaag tagaaggttc
     2221 gctggccggg gacgccgacg gcgtcggcgg tgatgcgctc gacgggatcg aactccaccg
     2281 ccctatcctg ccacccgccg ccggaacgtc accgcttccc gggaccacgg gccgtgcgcg
     2341 gaggcggcgc cgacggcacc aggtctgcga gcgtgccggt gtcgttcacc cgcagcaggc
     2401 ggggcggatg ctccccgagc gcgaccgcgg tcaccgaggc cggggagacg atcacgcgct
     2461 ggaacaggtc gaggtgaacc ccggagtagt gcgcgatcag caggcggatg acgtcggcgt
     2521 ggctgcaggc caccaccgta ccgcggcggt gcgcggtgac gatgcggtcc accgccgcca
     2581 cggcccgcgc ctgcacctcg ccgaacgtct cgcccccggg gaagcgcacg cgcgagggcg
     2641 tctcgcgcac ggcagtccac aggcggcggc ggagcagctg ccggaacgtc ttccccgacc
     2701 attcgccgta gccgacctcg atcaggccgg gctctggtcg caggg
//
LOCUS       DASPWO010000529         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_754715, whole genome shotgun sequence.
ACCESSION   DASPWO010000529 DASPWO010000000
VERSION     DASPWO010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754715"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1161
                     /gene="gcvT"
                     /locus_tag="VEO00_12135"
     CDS             64..1161
                     /gene="gcvT"
                     /locus_tag="VEO00_12135"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HYU58782.1"
                     /translation="MSDGKLKRTPLEAEHVALGAKMGPFAGWSMPIEYSGTLAEHRAV
                     REAVGLFDLTHLGRVTLAGEGALDVLLHAFTNDASSLADGRAQYTLCLNEEGGIVDDL
                     IVYRTGGTRYLVVPNAANRDRVFELMLEGVPAPEHATIREDTVLLAVQGPRSTGLVVR
                     FFPVAADLAYMHCAEVEHEGRPLILARSGYTGEVGFEIVVAETQAAGLWRRLLEEGEA
                     LGIVPCGLGARDTLRLEMGYPLHGNDISPDRTPLEAGLSWAVHWEHGFRGREPLLRQK
                     EAGIASRLWGLRMEGRLIPRAHYPVFAGDEQVGETTSGTFSPTLRVGIALAYLSPRER
                     FSPGDRVEVDVRGRRGAAEVVRPPFVERSPK"
     regulatory      1222..1373
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1421..>2170
                     /locus_tag="VEO00_12140"
     CDS             1421..>2170
                     /locus_tag="VEO00_12140"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HYU58783.1"
                     /translation="MSRAPRARLTLLGALAAIAIVASACVPQPSATTQDSPLPAFPVT
                     LTDDEEVTVTLDAVPKRIVTWAPSNTEVLFALGIGERVVGVSGEFDDYPPEARRIERV
                     GGANVQPNVETVVALQPDLVLEGFGGGEEWKQQLREQGIPVFTVVAAGFEDLLHDIRT
                     IGRLTGTTAKAGTLVNDMRGRAEDVAGRVADEERVTCFFEVGFEGGFFTVGPGSLIYD
                     LLLRAGCDPVTADADSAFPQWSVEELVADDPD"
BASE COUNT          297 a          739 c          833 g          301 t
ORIGIN      
        1 cgaaccggtc cgatggccat actaccgccg acgcggaatc ccccggtcca tgaggagggc
       61 agtgtgtcgg acgggaagct gaagcggacg ccgctcgagg cggagcacgt cgcgctcgga
      121 gcgaagatgg ggccgttcgc cggttggtcc atgccgatcg agtactcggg cacgctcgcg
      181 gagcaccgcg ccgtgcgcga ggccgtcggc ctcttcgacc tcacccacct cggccgggtc
      241 acgctggccg gcgagggcgc gctggacgtg ctgctgcacg ccttcacgaa cgacgcctcg
      301 tccctcgccg acggccgggc acagtacacg ctgtgcctga acgaggaggg gggcatcgtc
      361 gacgacctga tcgtctaccg caccggtggt acgcgctacc tcgtggtgcc caacgcggcg
      421 aaccgcgacc gggtgttcga gctgatgctg gagggcgtcc cggcgccgga acacgccacg
      481 atccgcgagg acaccgtgct gctcgcggtg caggggccgc gctcgaccgg gctggtcgtg
      541 cggttcttcc cggtcgccgc cgacctcgcg tacatgcact gcgccgaggt cgagcacgag
      601 gggcgaccgc tcatcctggc ccgctccggc tacacgggcg aggtgggctt cgagatcgtg
      661 gtcgcggaga cccaggcggc cggcctgtgg cgccggcttc tggaggaggg agaagcgctc
      721 ggcatcgtgc cgtgcgggct cggggcgcgc gacaccttgc gcctggagat ggggtacccc
      781 ctgcacggga acgacatctc gcccgaccgg acgccgctcg aggcggggct gtcctgggcg
      841 gtgcactggg agcacggttt ccgcggccgc gagccgctgc tgcggcagaa ggaggccggg
      901 attgcctcgc ggctgtgggg gctgcgcatg gagggccggc tgatcccgcg agcgcactac
      961 ccggtcttcg ccggcgacga gcaggtcggg gagacgacca gcggcacgtt ctcgccgacg
     1021 ctgcgggtcg ggatcgcgct ggcctacctc agcccgcggg agcggttctc gcccggcgac
     1081 cgggtcgagg tggacgtccg cggccgccgc ggcgcggcgg aggtcgtccg gccgccgttc
     1141 gtcgagcgct cgccgaagta gggacgcgta gaatccgcgg tgcaaggaca tcgcgagccc
     1201 cgcggtggtc cgggtgggga cggcatcccc gtccggctga agagggaagc cggtgagaat
     1261 ccggcgcggt cgcgccactg tgaccgggga gcgaccccga gagatgccac tgggaggcca
     1321 ggcctcccgg gaaggttcgg gggagcgatg atccgggagc caggagacct gcccggggcc
     1381 gcccgatacg gccgcgcgtt cggccagagg ggaggaccgc atgtctcggg ccccacgagc
     1441 ccggctcact ctgctcggtg ccctggccgc catcgcgatc gtggcgtccg cctgcgtgcc
     1501 gcagccgtcg gccacgacgc aggattcgcc gctcccggcg ttcccggtca cgctgaccga
     1561 cgacgaggag gtcacggtga cgctcgacgc cgtgccgaaa cgcatcgtca cctgggcgcc
     1621 gtcgaacacg gaggtcctgt tcgcgctggg gatcggggag cgggtcgtcg gcgtctccgg
     1681 cgagttcgac gactacccgc cggaggcgcg ccggatcgag cgcgttggcg gcgcgaacgt
     1741 tcagccgaac gtcgagaccg tggtggcact gcagccggac ctggtcctcg aggggttcgg
     1801 cgggggtgag gaatggaagc agcagctccg ggagcagggc atcccggtgt tcacggtcgt
     1861 cgccgccggc ttcgaggacc tgttgcacga catccgcacg atcggccggc tgaccgggac
     1921 cacggccaag gcaggcacgc tggtgaacga catgcgcggc cgggccgagg acgtcgccgg
     1981 gcgcgtcgcc gacgaggagc gcgtgacctg cttcttcgag gtcggcttcg agggtggctt
     2041 cttcacggtg ggccccggct cgctgatcta cgacctcctg ctgcgcgcgg gctgtgaccc
     2101 ggtcaccgcc gatgccgact cggccttccc gcagtggtcg gtcgaggagc tcgtcgccga
     2161 cgaccccgac
//
LOCUS       DASPWO010000530         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_498387, whole genome shotgun sequence.
ACCESSION   DASPWO010000530 DASPWO010000000
VERSION     DASPWO010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498387"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..988
                     /locus_tag="VEO00_12145"
     CDS             47..988
                     /locus_tag="VEO00_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HYU58784.1"
                     /translation="MAAALGTTLPPAPDREQVAGKLIRASQDHSFDPMVDVDWEAPLA
                     PAAYAEPPHRSSLYGTHLWDRLDQDRRVLLTRHEVASIASVGIWFETILLQMMARHIY
                     NLDPRTAHVQYALTEVGDECRHSVMFARMIEKLGCPVYGPGRVAHAMGRVFKATSTGP
                     LTFGAALFVEELLDSLQREAMRDETLEPLVRNVSRIHVIEESRHIRYARAELGRQWSR
                     LSAPARETTRIQLAGAATVATRRLIHPRAYAAVGLDPRTAHRAAVRNPHWRGTLAWSA
                     RKVVDDFTGLGLIRGPARLLWNRAGLLDWVDGGDAAA"
     gene            1108..1329
                     /locus_tag="VEO00_12150"
                     /pseudo
     CDS             1108..1329
                     /locus_tag="VEO00_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyacetophenone monooxygenase"
     gene            complement(1642..>2221)
                     /locus_tag="VEO00_12155"
     CDS             complement(1642..>2221)
                     /locus_tag="VEO00_12155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYU58785.1"
                     /translation="VLWLIGITFVFNFLYGPIDVGLPLFVRNTLGAGPALYGQMFTVF
                     GLGSLVGGLAVGSLRRASVVRWITVVSIFGWGGAALLLAVAGTPSVALLALGIGGLVY
                     GPFTAASVTAIQAATPVADVGAVISIWAAITIGALPLGTAVGGPLVAAMGTRGTFAIC
                     AALTIVLGIATLPRLLASPRPPAERRPTVRSA"
BASE COUNT          355 a          765 c          801 g          300 t
ORIGIN      
        1 tgcattgtca agatcagggg agtacaggac gaaggaggtt cgctcgatgg ctgcagcgct
       61 cggcacgacg ctcccccccg ccccggatcg cgagcaggtg gccggcaagc tgatccgggc
      121 ctcccaggac cactccttcg accccatggt cgacgtggac tgggaggcgc cgctcgctcc
      181 ggccgcctac gcggagcccc cgcaccgctc ctcgctgtac gggacccatc tttgggatcg
      241 cctcgatcag gatcggcggg tgctgctcac tcgccacgag gtggcgagca tcgcgagcgt
      301 tggcatctgg ttcgagacga tcctgctcca gatgatggcc cgccacatct acaacctcga
      361 tccgaggacc gcccacgtcc agtacgccct caccgaggtg ggtgacgagt gccggcactc
      421 cgtgatgttc gctcggatga tcgagaagct cggatgcccg gtctacggac cggggcgtgt
      481 cgcccacgcc atgggtcggg tgttcaaggc gaccagcacc ggaccgctga cgttcggcgc
      541 cgccctgttc gtggaggagc tgctcgactc gctgcagcga gaggccatgc gcgacgagac
      601 gctcgagccg ttggtccgga acgtctcgag gatccacgtg atcgaggagt cccggcacat
      661 ccggtatgcc cgggccgagc tcggccggca gtggtcccgg ctctcggcgc cggcgcgtga
      721 gacgacacgg atccagctgg ccggggcggc caccgtcgcg acgcggcgac tgatccaccc
      781 ccgggcgtac gcggcggtcg gcctcgaccc ccgcaccgcg caccgtgccg ccgtgcgaaa
      841 cccccactgg cgaggaacgc tcgcatggtc ggcccgcaag gtcgtggacg acttcaccgg
      901 gctgggcttg atccgggggc cggctcgctt gctgtggaac cgcgccggcc tgctcgactg
      961 ggtcgacggg ggggacgcgg cggcgtgagg gcgacgatcg agcgcgcccg cgcctcgcag
     1021 cgggggtacg aggcgagtcg agccgacgat gacggcgcgc cgcacattcg cgaggcggag
     1081 cccatgcccg atgtccaggc ccgggacaac cgagacctcc agaggccgat gcggcggacc
     1141 gtctggctga atggaggatg ccgcagctgg tacctcgacg cgaacggccg caacacgacg
     1201 ctgtggcccc ggtcgacgtg gacgttccgc cggttgacgc ggcggttcga ccccgcgagc
     1261 tatcgcctgg tcgcgcggcc ggaggccgtg cgtgcgtcgg gggcgctggc gagcgcccga
     1321 tcgtcctagg gtcgcctggc gacgagtctc ggctgcagcc gttcgtggac cgaacaggtc
     1381 gccgactcgt tgtagatcga gagcactgtg gagcagccat cggagacaca gacccgccct
     1441 gactgcgatc gccggagcgc cttgtgtcga tcgatcgaac cgaacttgac ctggctgttg
     1501 ctcgccacgg cgcctcccgc tggtacggtc cgagggagcg aggaaggtcg tgctgggtcg
     1561 cccacgcgaa cggcaggtcg gtcctgctgg ccatcactgt acctggttgg ggcgcggccg
     1621 ggcgcgtcta cgcggtctcg ctcaagcgga cctgacggtc ggcctcctct ccgcgggagg
     1681 acgaggggag gcgaggaggc gagggagcgt cgcgatcccg aggacgatgg tgagggcggc
     1741 gcagatcgcg aaggttccac gggtccccat ggccgccacg agtggccctc cgacggcggt
     1801 gccgagcggc agggcgccga tcgtgatcgc cgcccagatc gagatcacgg cgcccacatc
     1861 ggcgaccgga gtggccgcct gaatggcggt cacgctcgcg gcggtgaagg gcccgtagac
     1921 caggcctccg atccccaggg ccaggagcgc gacagacgga gtgccggcca ccgcgagcag
     1981 cagggccgct ccgccccacc cgaagatcga gacgacggtg atccaccgca cgaccgaggc
     2041 ccgccgaagg ctccccacgg ccaggccgcc gaccagcgag cccagtccga acaccgtgaa
     2101 catctgcccg tagagggccg gccccgcacc cagggtgttc cgaacgaaga gcggaagccc
     2161 gacgtcgatc ggcccgtaca ggaagttgaa gacgaacgtg atgccgatca gccacaggac
     2221 g
//
LOCUS       DASPWO010000531         7166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_668037, whole genome shotgun sequence.
ACCESSION   DASPWO010000531 DASPWO010000000
VERSION     DASPWO010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668037"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VEO00_12160"
     CDS             complement(<1..622)
                     /locus_tag="VEO00_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58786.1"
                     /translation="MDRESSDNLSAAEVAERAGVTEADVERMAALGILEPHRKFGPGD
                     VRRVRLAMACERAGLPMDAIAESVRENRLSFAFLDAVAYRRWAERTTRTYADVVEEAG
                     VSFDTLRACLEAMGFAPMEPMDRIRADELEIVPLIQLSLATGVMDEAWFVRLGRAYSE
                     GLRSLAQAENEVYHWRFEAPFLEQGMTQAQAMERASQMAGQYNDLVD"
     gene            1048..1335
                     /locus_tag="VEO00_12165"
     CDS             1048..1335
                     /locus_tag="VEO00_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58787.1"
                     /translation="MDVLAEVARGRSNRGVGKALSISEEAVKSRRSLIWAERTRDCGV
                     VDHGVADPAPLEGSMTGSKMDSATGDQVPPSAGVPSSPAAPCSRRAAARNR"
     gene            complement(1257..2096)
                     /locus_tag="VEO00_12170"
     CDS             complement(1257..2096)
                     /locus_tag="VEO00_12170"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF014752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HYU58788.1"
                     /translation="MERDEFVVARNPEPDSSLPYLLRLPIDGGLVLKAREPWPATSRV
                     YCHPAEGWPDDPEIVESAPVRLCRRRGAAVDLVLDRARNNRAQFVFTEIKSRPAIFWQ
                     TAKSLRSARPGVRVPSRKAAGLRDWRIQVDTREHYPYRFAKHPSVTAVRAALPAGDYA
                     VRDGDDGAILAAVERKTVPDMSKGLVEGSLAFTMAELAILPAAAVVVEGRYSDLFKVP
                     RVQPGWLPEVLARLQVRYHDVPVVFAETRPLAEEWTYRFLAAALREHGAAGEDGTPAE
                     GGT"
     gene            complement(2185..2589)
                     /locus_tag="VEO00_12175"
     CDS             complement(2185..2589)
                     /locus_tag="VEO00_12175"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HYU58789.1"
                     /translation="MILVDTSAWIHFLRGTDHPVRFVLRDFLREPGQLATTPPVVMEV
                     LAGARSDHEAAALRARLLGLQFLPIVGLRGYEEAAAIHRACRGGGVTVRSQIDCLIAA
                     VAIRNDASVLHADAGFEMIARHTPLRLEPLGS"
     gene            complement(2586..2795)
                     /locus_tag="VEO00_12180"
     CDS             complement(2586..2795)
                     /locus_tag="VEO00_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017603793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HYU58790.1"
                     /translation="MGRTNVVVDDELVEKVMRLYGLRTRREAIDFALRAVAGGDGTLS
                     MLDLEGSGWEGDLFEMRRSEPIEDL"
     gene            2961..4685
                     /locus_tag="VEO00_12185"
     CDS             2961..4685
                     /locus_tag="VEO00_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58791.1"
                     /translation="MARSLRGRRFLAGLASGVVAVSVVVVPRVASASEVTWAAMFGSA
                     NRDHGTAVSATTGAVYVGGVTSGQLPTLTSKGKDDAFLRKFDASGAVQWTVQFGTNNY
                     DAVMGISARTDGVYAGGFTLGSLYGASAGGVDAIVRKYDTSGGVLWTRQFGTAADEAV
                     LAVTSDADGVVVAGWTSGELQGSSAGKTDAFVRKFDASGNAVWTRQFGGSGDERAEGV
                     SLTSGNVYVGGYTTAGLPSEAYGGGRDGFIRKYDSAGNAQWTDEIGTAGTDDVSGVSA
                     RVGGIYVSGFTDGTLPGQANAGGTDGFVWKYSSGGSTAWAKQFGTSGGDVALAVAATS
                     SREYVAGYTSGTFAGESLAGDTDSFVVTVDTGGSIIRTREFGTSGWEGAMDVSAPSSS
                     AAYFAGAGNKALFGHANSGGFDAFAGKYVLSRPDGLIGTTAASQIGNGVYNTTGTGQT
                     KTGQAETFDSVTFFITAQNDGPAAESMKVTGCGGSPGWGVKYLAGTVGGADITSQVVA
                     GTYSLGSLAPGDAKSLRVQILHKSGAQIGSYKSCLVTVRSSTQSTARDAVKARVKAVA
                     PTTPEAAR"
     gene            4682..>7166
                     /locus_tag="VEO00_12190"
     CDS             4682..>7166
                     /locus_tag="VEO00_12190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYU58792.1"
                     /translation="MRVALARAKPRGVLLAAAVAALAAQAVGVPAARAADPPDTVITS
                     GPFGEVVPGPLWFEFTASERDLRFECSLDEEAFADCSSPQAYDGLPRGDHTFLVRAVS
                     GAGADPTPDGRSFTVGRSPCQRLEPVGRPVALGCSVIELMRRTGAFVNPVVDGLRRPL
                     AKLQGRAGFAGAVNDLTDAIAQLRSAGEHLGEGQPCPAGRDAGLAAANLRSARTKIGN
                     ALDRMQQDLADETSLPEGDADRLDLLISELRLQQVLADDAISDGLGVADAFDAACSAV
                     TGPVEVRGRVVRTDDAQGTFELEDGSVFGLPLTRHGSALVKGRVVDVGGLGLEDRTAV
                     VTSVEGTSAKKVAAVQCLRLRIAPVQKFSGGPITLLSTSAYESGGVLQLETAMRLGVQ
                     ETGCPGATPEGHALHYTMKIDVVQGGETDTIATDLGEGEGAVPFPWPLSQGGSAAVDV
                     TVIRTNCTVIGPDFESCQPPQILSQDSFDVLLRQVGSYSDTEYDTTVFGVTDDGVNGN
                     FEPASVTGFDLHFVSSITNPVFHADGYKVNGGSSTKPQVVAIDEGDKFAVYDTDFYDP
                     GYLWLEDEVLATGQDSPAGIRWPRIVGTRNGHQYRYFSSLPSIVRDRVAVCPTYPSLT
                     IPQGPPIPPEDPAWNYFYPNYPPREAYTTKTVKDSFYKLPFEKNFQPGTGLMNIDDDK
                     VTARHPEWQPYAFDLTAPESTKLLATRGGTVVINVHDDPYNVNDAAKPANWPGIGNYM
                     WLTHGDGTYAVYFHMKFNSNVVWTGNEVSRGDQISLIGQTGNASGPHTHFEVTEKTPP
                     VPDSQHTRVRFQVLFGANKVQKNCYIPRKG"
BASE COUNT         1103 a         2493 c         2567 g         1003 t
ORIGIN      
        1 ggtccaccag gtcgttgtat tggcccgcca tctgggaggc ccgctccatg gcctgggcct
       61 gggtcatgcc ctgctcgagg aacggcgcct cgaaccgcca gtggtagacc tcgttctcgg
      121 cctgggccag gctccgcagg ccctccgagt acgcccgccc caaccgaacg aaccacgcct
      181 cgtccatgac gccggtggcg aggctgagct ggatgagcgg cacgatctcg agctcgtccg
      241 cccggatccg gtccatcggc tccatcgggg cgaaccccat cgcctccaga caggcgcgaa
      301 gcgtgtcgaa cgagacgccg gcctcctcga cgacgtccgc gtaggtgcgc gtggtccgct
      361 cggcccaccg ccggtaggcc actgcatcca ggaacgcgaa cgacagccgg ttctcacgaa
      421 cggactccgc gatcgcgtcc atcgggagcc cggcccgctc gcacgccatg gccaggcgca
      481 cgcgccggac gtcaccgggc ccgaacttcc ggtggggctc gaggatcccg agagccgcca
      541 tccgctcgac gtccgcctcg gtgaccccgg cccgctcggc cacctcggcg gcggacaggt
      601 tgtctgagga ctctcgatcc atgacccgag catcgtacga gggcacgccc ccgcgtctgt
      661 ggttcaggag cgactcgctc gctcgggaat gggagtagcc tcggggccgc gctcttcggc
      721 gggggcgacg gcgtcccgac ccgttcttcc cgccggggga gagtctggcc tgcggggccg
      781 tgatccagtc cgacggcagg atcgtcctgg ccggctacgc ggcggccaac gccaccaccc
      841 acgggttcgg catggagcgg ttcctgggct gatcagctcc gcggccgggg tggtggctcc
      901 ccgggtcaat cgcctcgaag acgcggacgg gctccgcgaa gccggcgcgc ggtgagagcg
      961 tcccttcgcc atcgttcatc gcccgcctga tcaaggaggt acgcgaaggc ccaaccggcc
     1021 ccctgccccc ctcagcgctc ccgcgagatc gacgtcctcg cagaggtggc gcggggccgc
     1081 tccaaccgcg gggtcgggaa ggccctctcg atcagcgaag aggccgtgaa gagccggcgc
     1141 tccttgattt gggctgaacg gacgcgggat tgcggggtcg tcgaccacgg cgtagcggat
     1201 cccgccccac tcgagggctc gatgaccggc tcgaagatgg actcggccac gggggatcag
     1261 gtcccaccct cggccggggt gccgtcctcg ccggcggcgc cgtgttcgcg gagggcggcg
     1321 gcgaggaacc ggtaggtcca ttcctccgcg agcggcctgg tctccgcgaa cacgaccggc
     1381 acgtcgtggt accggacctg cagccgggcc aggacctcgg ggagccagcc cggctggacg
     1441 cgggggacct tgaacaggtc ggagtaccgg ccctcgacca cgacggccgc ggccgggagg
     1501 atcgcgagct cggccatcgt gaaggccagc gagccctcga ccaggccctt cgacatgtcc
     1561 gggacggtct tgcgctcgac cgcggcgagg atggcgccgt cgtccccgtc gcgcaccgcg
     1621 tagtcgccgg cgggcagcgc cgccctgacg gcggtcaccg acgggtgctt ggcgaaccgg
     1681 tacgggtagt gctcgcgcgt atcgacctgg atccgccagt cgcggaggcc ggccgccttc
     1741 cgcgacggga cgcggacgcc gggccgggcc gagcggagag acttcgccgt ctgccagaag
     1801 atcgccgggc ggctcttgat ctcggtgaac acgaactggg ccctgttgtt gcgggcgcgg
     1861 tccaggacga ggtccaccgc cgccccgcgc cggcgacaaa ggcggactgg cgcggactcg
     1921 acgatctcgg ggtcgtcggg ccacccctcg gccggatggc agtacacgcg cgaggtcgcc
     1981 ggccacggct ccctggcctt cagcacgagc ccgccgtcga tgggcagccg cagcagatac
     2041 ggcagcgagc tgtccggctc cgggttccgc gccaccacga actcgtcccg ctccacggac
     2101 ccgattatcg cccccgaggg ggcgatctct gctccatgtg cgcagccggc ccgtgatgcg
     2161 ggccgccgaa gttcgcctcc gtcctcagct cccgaggggc tcgagccgca gcggggtgtg
     2221 tcgggcgatc atctcgaagc ccgcgtcggc gtgcagcacc gaggcgtcgt ttcggatggc
     2281 gaccgcggcg atcaggcagt cgatctggct acggacggtc acgccgccgc ctcgacacgc
     2341 ccggtggatc gcggccgcct cctcgtagcc gcgaagaccg acgatcggca ggaactgcaa
     2401 cccgagcaac cgggcccgca acgcagcggc ctcgtggtcc gatcgagcac ccgccagcac
     2461 ctccatcacc accggcgggg tcgtggccag ttgcccgggt tcgcgcagga agtcgcgcag
     2521 cacgaatcga accgggtggt cggtcccgcg caggaaatgg atccacgcgg acgtatcgac
     2581 caggatcaca ggtcctcgat cggctcgctc cgacgcatct cgaagagatc gccctcccag
     2641 ccgctccctt ccaggtcgag catgctgagc gtcccgtcgc ctcctgccac ggcgcgcagg
     2701 gcgaagtcga tcgcctcgcg cctggtccgc aagccgtaca ggcgcatcac cttctcgacc
     2761 agctcgtcgt ccaccacgac gttcgtcctg cccacgaccc aatcgtacac atcaatacac
     2821 atcagtacac accgctccct gcccatacat ggagccaccg acacacgctc attccccccc
     2881 aggatgtcga acctcggtgg acagcggggg agcggcctgc cttgacgggc ttcgggaagg
     2941 agcaaagact cgcggtgtcc atggcgcgct ccctccgcgg tcgtcggttc cttgctggtc
     3001 tggcctcggg tgtggtcgcc gtctccgtcg tggtcgttcc ccgggtggcc tcggcgagcg
     3061 aggtgacgtg ggccgcgatg ttcgggtccg cgaaccggga ccatgggacg gccgtgtcgg
     3121 cgaccaccgg cgccgtctac gtgggcggcg tgacgagcgg ccagctcccg acgctcacca
     3181 gcaagggcaa ggacgacgcc ttcctccgga agttcgatgc ctccggcgcg gtgcagtgga
     3241 ccgtccagtt cggcacgaac aactacgacg cggtgatggg gatctccgcg aggaccgacg
     3301 gcgtctacgc gggcggcttc accctcggct cgctctacgg agccagcgcc ggcggcgttg
     3361 acgcgatcgt ccgaaagtac gacacgtccg ggggcgtgct gtggacgagg cagttcggca
     3421 cggccgccga cgaggcggtc ctcgcggtga cctcggacgc cgacggtgtg gtggtggcgg
     3481 gttggacgag cggtgagctg caaggctcca gcgccggcaa gaccgacgcg ttcgtccgga
     3541 agttcgacgc ctccggaaac gcggtgtgga cccggcagtt cggcggctcg ggcgacgaac
     3601 gggccgaggg cgtgtcgctg acctcgggga acgtctacgt gggcggctac acgacggccg
     3661 gactgccgag cgaggcgtac ggcggcgggc gggacggctt catccggaag tacgactcgg
     3721 cgggcaacgc gcagtggacc gacgagatcg gcacggccgg aacggacgac gtgtccggcg
     3781 tctcggcgcg ggtgggcggc atctacgtct cggggttcac ggacggcacg ctgcccggcc
     3841 aggcgaacgc cggcgggacc gacgggttcg tctggaagta ctcgtcgggc gggtccacgg
     3901 cgtgggcgaa gcagttcggc acgtccggtg gggacgtcgc gctggccgtc gcggccacct
     3961 cgagccggga gtacgtggcc gggtacacga gcgggacgtt cgccggcgag agtctcgcgg
     4021 gggacaccga ttcgttcgtg gtgacggtcg acaccggggg gagcatcatt cgaacgcgcg
     4081 agttcggcac gtccgggtgg gagggcgcga tggacgtgtc ggcgccttcg tcctcggcgg
     4141 cctatttcgc gggcgcgggg aacaaggcgc tgttcggaca cgcgaactcc ggcgggttcg
     4201 acgcgttcgc cgggaagtac gtgctgtcgc ggcccgacgg cctgatcggg accacggcag
     4261 cgtcgcagat cgggaatggc gtctacaaca ccaccggcac aggccaaacg aagaccggcc
     4321 aggcggagac gttcgactcg gtcacgttct tcatcaccgc ccagaacgac ggcccggccg
     4381 cggagtcgat gaaggtgacc gggtgcgggg gttcgcccgg gtggggcgtg aagtacctcg
     4441 cggggacggt ggggggcgcc gacatcacga gccaggtcgt cgccgggacc tactcgctgg
     4501 ggtcgctcgc gccgggggac gcgaagagcc tgcgcgtgca gatcctgcac aagagcggag
     4561 cgcagatcgg ctcgtacaag agctgcctgg tcaccgtccg gtcctcgacc cagagcaccg
     4621 cgagggacgc ggtgaaggcc agggtgaagg ccgtggcgcc gacgacgccg gaggctgctc
     4681 gatgagggtt gcgctggcgc gggcaaagcc ccggggcgtc ctgctggccg ctgccgtggc
     4741 ggcgctggcc gcgcaggccg tcggggtgcc cgcggcccgg gccgccgatc cgcccgacac
     4801 cgtcatcacc tcgggaccct tcggcgaggt tgtcccgggc ccgctgtggt tcgagttcac
     4861 cgccagtgag cgcgacctcc ggttcgagtg ctcgctggac gaagaggctt tcgccgactg
     4921 ctcctcgcca caggcgtacg acgggctgcc ccggggcgac cacacgttcc tggtgcgggc
     4981 ggtgtccggg gccggggccg atccgactcc ggacggacgt tcgttcaccg tcggccgaag
     5041 cccgtgccag cgcctcgagc cggtgggccg gccggtcgcc ctggggtgct cggtcatcga
     5101 gctcatgcgc aggacggggg cgttcgtgaa cccggtcgtg gacggcctcc ggaggccgct
     5161 cgccaagctg caggggcgtg ccgggttcgc cggcgcggtg aacgacctca ccgacgcgat
     5221 cgcgcagctg aggtcggccg gggagcacct gggggagggg cagccgtgcc cggccggtcg
     5281 ggacgccggc ctggcggctg cgaacctgcg aagcgcccgc acgaagatcg gcaacgcgct
     5341 ggaccgcatg cagcaggacc tcgccgacga gaccagcctc ccggaggggg acgccgatcg
     5401 gctggacctc ctcatctcgg agctgcggct gcagcaggtg ctcgcggacg acgccatctc
     5461 cgacggcctc ggtgtcgcgg acgcgttcga cgcagcatgc tcggcggtga ccgggccggt
     5521 cgaggtccgc ggccgcgtgg tccgaaccga cgacgcccaa ggcacgttcg agctggagga
     5581 cggttcggtc ttcgggttgc ccttgacgcg ccacggctcg gcgctggtca aggggcgggt
     5641 cgtggacgtc ggcggcctgg ggctcgagga ccgaacggcc gtcgtcacgt cagtggaggg
     5701 gacctccgcg aagaaggtgg ccgcggttca gtgcctgaga ctgcgcatcg cgccggtcca
     5761 gaagttctcc ggcggtccga tcacgctgct gtccacctcg gcctacgaga gcggtggcgt
     5821 cctccagctc gagacggcca tgcgcctggg cgtacaggag acgggatgcc cgggggcgac
     5881 gcccgagggc cacgcgctgc actacacgat gaagatcgac gtggtgcagg gcggggagac
     5941 cgacacgatc gccaccgacc tcggcgaggg cgagggagcg gtccccttcc cgtggccgct
     6001 gtcccagggc gggtccgcgg cggtggacgt cacggtgatc cggacgaact gcacggtcat
     6061 cggccccgac ttcgagtcct gccagccgcc ccagatcctg tcgcaggact cgttcgacgt
     6121 cctgctgcgc caggtgggga gctactcgga caccgagtac gacaccacgg tgttcggcgt
     6181 caccgacgac ggggtgaacg ggaacttcga gccggcgtcg gtgaccgggt tcgacctcca
     6241 cttcgtgtcg tccatcacga acccggtctt ccacgcggac ggctacaagg tgaacggcgg
     6301 ctcgtcgacg aagccgcaag tggtcgcgat cgacgaaggg gacaagttcg ccgtgtacga
     6361 cacggacttc tacgaccccg gctacctgtg gctggaggac gaggtgctgg ccacgggcca
     6421 ggacagcccg gcggggatcc ggtggccgag gatcgtgggc acacgcaacg gccaccagta
     6481 ccgatacttc tcgtcgctgc cgtcgatcgt ccgggaccgg gtcgcggtgt gcccgacgta
     6541 cccgtcgctc acgatcccgc agggcccgcc gatcccgccc gaggaccccg cgtggaacta
     6601 cttctatccg aactacccgc cccgggaggc ctacacaacg aagaccgtga aggactcgtt
     6661 ctacaagctg ccgttcgaga agaacttcca gccggggacg ggcctcatga acatcgacga
     6721 cgacaaggtg acggctcgcc accccgagtg gcagccttac gcgttcgacc tcacggctcc
     6781 ggagagcacg aagctgctgg cgacgcgcgg cggcacggtg gtcatcaacg tgcacgacga
     6841 cccctacaac gtgaacgacg ccgcgaagcc cgcgaactgg cccgggatcg gcaactacat
     6901 gtggctcacc cacggcgacg gcacgtacgc cgtctacttc cacatgaagt tcaacagcaa
     6961 cgtggtctgg accggcaacg aggtgtcccg aggcgaccag atctccctca tcggccaaac
     7021 gggcaacgcg agcggccccc acacccactt cgaggtcacc gagaagaccc cacccgtccc
     7081 cgacagccaa cacacccggg tccgcttcca ggtcctgttc ggcgcgaaca aggtccagaa
     7141 gaactgctac atcccccgca agggcg
//
LOCUS       DASPWO010000532         8645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_498570, whole genome shotgun sequence.
ACCESSION   DASPWO010000532 DASPWO010000000
VERSION     DASPWO010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498570"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VEO00_12195"
     CDS             <1..386
                     /locus_tag="VEO00_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU58793.1"
                     /translation="APTFITDDLPAAREQVRWFPALVSNHVVDLVNRYASDELPAELT
                     DYIKARDHYDYLEHGQRGAEHAQFVPDDIVDRFCVIGTVEQCIEKLRVLEGLGVDQFT
                     IYAMSVEGPESIIETYGNEIIPKFA"
     gene            430..1275
                     /locus_tag="VEO00_12200"
     CDS             430..1275
                     /locus_tag="VEO00_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019391581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HYU58794.1"
                     /translation="MTTVRAAVIQANANMSKEEAVEKHVELIGKAAAEGAQVTCLQEI
                     FHGPYFPAEQDPKWYATAEPDDGPTVTRMREVARDHRMVLVVPFYEEAQTGVYYNAAV
                     VIESDGTIAGKYRKSHIPHVGPCFWEKFYFKPGNLGYPVFQTSVGRLGLIICYDRHFP
                     EIARALGLKGAEIVFNPSATVESLSKYLWKLEQPAHAVANGYWIGAINRVGVEAPLST
                     AKFYGTSYFCDPRGQIVAEASDSQDEVLVADLDLEMIREVRNTWQFLRDRRPETYGEL
                     TELLP"
     gene            1272..2591
                     /locus_tag="VEO00_12205"
     CDS             1272..2591
                     /locus_tag="VEO00_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYU58795.1"
                     /translation="MTEPFSGDDGGADELLARHREVMPSWLLLYYDRPIELVRGEGAR
                     VWDAAGREYLDFFGGIVTTISGHVVPKLVEALTEQANRIAHTSTLYLIRPMVELGERL
                     TGLAPVDPPAKAFFVGSGTEAIEAALLFATSWRRSNEIIALRNSYHGRSFGAVGITGN
                     KGWSATSFSPLNVSYALAPYCYRCPLGLTYPDCGVACAEDVRNVIETTTTGEPAAMIA
                     EPIQGVGGFVTPPPEYFGIVKSILDEFGIPFIADEVQTGFGRTGEAFWGIESYGVGAD
                     AVVCAKGLGNGMAIGAVIGRADMVDSLRANSISTFGGNPIASTYALANLDHIQEENLQ
                     QNAYKVGNYLFRGLKDLEDRFDVVGEVRGKGLMIGVELVKDGESKAPSPEAAGRVMEA
                     CRERGLLIGKGGLYGNVLRVSPPLTIGEDDAARAIETLEVAFGEASS"
     gene            2588..3964
                     /gene="hydA"
                     /locus_tag="VEO00_12210"
     CDS             2588..3964
                     /gene="hydA"
                     /locus_tag="VEO00_12210"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HYU58796.1"
                     /translation="MRTLIGGGTVVTASEATVADVLVQDERVAAVGVGLDAQADRTID
                     ASGRYVMPGGVDVHTHFDLPFGGSFCSDDFDTGTRAAAFGGTTTCVDFAVQDAGESLR
                     QGLDRWFEKGQKAQTDYGLHMIVREVNDGTLKEMDELVSEGITSFKLFMAYPGVFMLD
                     DASIFRAMQRAAESGALIMMHAENGGPIDVLVKQLIGEGKTEPRYHGASRPAAMEGEA
                     VHRVFKLAELAGTPAYIVHLSSRDALEAVREARDRGVAAYAETCPQYLYLSSDDMARP
                     GFEGAKYVCSPPLRPKDHQQDLWSGLAEGALQLVSTDHAPFNYVGQKDLGRDDFAKIP
                     NGLPSVEDRVTLMYQRVHAGQMSPSRWVELVATNPARMFGLYPRKGTIAPGFDADIVV
                     FDPNRERVLSAETHHMNVDYSCYEGMTVWGLPEVVLQRGTVLVESGEWLGKPGQGRFV
                     ARSRFQEP"
     gene            3976..5472
                     /locus_tag="VEO00_12215"
     CDS             3976..5472
                     /locus_tag="VEO00_12215"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HYU58797.1"
                     /translation="MMATRVQSLIGGRFRESSGEDVPVDPVFDPATGETIALLPYSTP
                     EEIDEAIRAASEAFPEWSNVPVPDRAQVMFRFKALLEEHFDELSRLVVRENGKTLPEA
                     SGEVRRAIEVVELACGAPSMLMGTNLDQIADGIDEELVRFPVGVCAGITPFNFPAMVP
                     LWMFPVALVAGNTFVLKPSQRTPLSAVRLAELLTEAGLPDGVFNVVHGAKDAVDALLT
                     HPDVAAVSFVGSVGVARYIYTTAAAHGKRVQALGGAKNHLIVMDDADLERSVTALISS
                     AFGNAGQRCLAGSVAVGVGSVGEALVRELRHQASALRLGPGEDPATDMGPVIREERRK
                     ELIGYIEEGQEAGATLALDGRGAGPARGFFLGPTIFDGVTPDMRLWKEELFGPVLSVL
                     RAADIDEALRVLNASTDGNAASIFTTSGHNAREFKRRAEAGMLGVNIGVAAPMAFFPF
                     TGWKSSFFGDLHATGADAFRFYTRQKVVTSRWWLAPTLDPTRPEGRGH"
     gene            5476..6258
                     /locus_tag="VEO00_12220"
     CDS             5476..6258
                     /locus_tag="VEO00_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010471886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYU58798.1"
                     /translation="MEPVYRPVAGLAYAVCWIMGWDIRVRGAEHVPRHGPAIVAANHV
                     GFLDFVFIGMGARRVRRWVRFMAFERAFRHWLGGPLLRGMRHIPVDRYGDARAALDLA
                     IRALRSGEVVGLHPESRINRTFVPTAGKSGAARMALETAAPLIPCAVWGSQRLWTRGR
                     RELRRGVMVTVDFGPSIAYRPGEPPAEVTERLMGEIRRLAGRAAERYPQRPRGPADRW
                     WLPAHLGGTAPPADSFVIGPPGPDRARDGRHSFEETDVTLGS"
     gene            6335..6508
                     /locus_tag="VEO00_12225"
     CDS             6335..6508
                     /locus_tag="VEO00_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58799.1"
                     /translation="MMQPGSLANYRIQDDLRRAEAYRRSKLTREGAAREQRAHGRSVG
                     RAILSALLWPIRH"
     gene            6645..6950
                     /locus_tag="VEO00_12230"
                     /pseudo
     CDS             6645..6950
                     /locus_tag="VEO00_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
     gene            7009..7359
                     /locus_tag="VEO00_12235"
     CDS             7009..7359
                     /locus_tag="VEO00_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HYU58800.1"
                     /translation="MEFGRFTFGSIEIDGTTYEHDLVIDRGRVRKRKKGPSKELRSGY
                     GHTPLSAAEDIPWTCRRLVVGSGADGALPVMDQVADEARRRGVELVVQPTARALETLR
                     AGSDDTNAILHVTC"
     gene            complement(7364..7675)
                     /locus_tag="VEO00_12240"
     CDS             complement(7364..7675)
                     /locus_tag="VEO00_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58801.1"
                     /translation="MPGIPEPAPFDGEAPEELDLAGFGAVVFAGGFRPDYRDLVTVPG
                     AFDDMGFPIQTDGASTVVPGLYFAGVHFQRKRKSATFFGIAEDAAIVARGIVERSSRP
                     R"
BASE COUNT         1287 a         2976 c         3203 g         1179 t
ORIGIN      
        1 cggcgccgac gttcatcacc gacgacctgc cggccgcgcg cgagcaggtt cgctggttcc
       61 cggcgctcgt ctcgaaccac gtcgtcgacc tggtgaaccg ctacgcgagc gacgagctgc
      121 cggcggagct gaccgactac atcaaggccc gcgaccacta cgactacctc gagcacgggc
      181 agcgcggggc ggagcacgcg cagttcgtcc ccgacgacat cgtcgatcgc ttctgcgtga
      241 tcgggacggt cgagcagtgc atcgagaagc tccgcgtcct cgaggggctc ggcgtcgacc
      301 agttcacgat ctatgccatg tcggtcgagg gcccggagtc gatcatcgag acgtacggga
      361 acgagatcat cccgaagttc gcgtgaccgt ccggtgcggc gacgacgcgg gacagagagg
      421 agggcgagca tgaccacggt gcgagcggcc gtgatccagg cgaacgccaa catgtcgaag
      481 gaggaggcgg tcgagaagca cgtcgagctg atcggcaagg ccgccgccga gggggcgcag
      541 gtgacctgcc tgcaggagat cttccacggc ccgtacttcc cggccgagca ggacccgaag
      601 tggtacgcga ccgccgagcc cgacgacggc cccaccgtga cccggatgcg cgaggtcgcc
      661 cgcgaccacc gcatggtgct cgtcgtcccg ttctacgagg aggcgcagac cggcgtctac
      721 tacaacgccg cggtggtgat cgagtccgac gggacgatcg ccggcaagta ccgcaagtcc
      781 cacatcccgc acgtcggccc gtgcttctgg gagaagttct acttcaagcc cggcaacctg
      841 gggtacccgg tgttccagac gtcggtcggg cggctcggcc tgatcatctg ctacgaccgg
      901 cacttcccgg agatcgcccg ggcgctcggc ctgaaggggg cggagatcgt cttcaacccc
      961 tcggccaccg tcgagtcgct ctcgaagtac ctctggaagc tggagcagcc ggcgcacgcg
     1021 gtggcgaacg gctactggat cggggcgatc aaccgcgtcg gggtggaggc gccgctgtcg
     1081 acggcaaagt tctacggcac gtcgtacttc tgcgacccgc gcggccagat cgtcgccgag
     1141 gcgtcggact cccaggacga ggtgctggtc gccgacctcg acctggagat gatccgcgag
     1201 gtccgcaaca cctggcagtt cctccgcgac cgccgcccgg agacctacgg cgagttgacg
     1261 gagctgctgc catgaccgaa ccgttctccg gggacgacgg cggcgccgac gagctgctgg
     1321 cccggcaccg cgaggtgatg ccctcctggc tgctgctgta ctacgaccgg ccgatcgagc
     1381 tcgtccgggg cgagggggcg cgggtgtggg acgctgccgg gcgcgagtac ctcgacttct
     1441 tcggtggcat cgtcacgacg atctccggcc acgtggtccc gaagctcgtc gaggcgctga
     1501 ccgagcaggc gaaccggatc gcgcacacgt cgacgctcta cctgatccgg ccgatggtgg
     1561 agctcgggga gcggctcacc ggcctggcac cggtggaccc gccggcgaag gccttcttcg
     1621 tcgggtcggg caccgaggcg atcgaggcgg cgctgctgtt cgcgacgtcg tggcgccggt
     1681 cgaacgagat catcgcgctg cgcaactcct accacggccg gtcgttcggg gcggtgggga
     1741 tcaccgggaa caagggctgg tcggccacct cgttctcgcc cctgaacgtc tcctacgcgc
     1801 tggcgcccta ctgctaccga tgcccgctgg gactcacgta cccggactgc ggggtggcct
     1861 gtgccgagga cgtccggaac gtcatcgaga cgacgaccac cggggagccg gcggcgatga
     1921 tcgccgagcc gatccagggc gtcggcgggt tcgtcacccc gccgccggag tacttcggga
     1981 tcgtgaaatc gatcctcgac gagttcggga tcccgttcat cgccgacgag gtgcagacgg
     2041 ggttcggccg caccggcgag gcgttctggg ggatcgagtc gtacggcgtt ggcgcggacg
     2101 ccgtggtgtg cgccaagggc ctgggcaacg ggatggcgat cggcgcggtg atcggccggg
     2161 ccgacatggt cgactccctg cgcgccaact cgatctccac cttcggcggg aacccgatcg
     2221 cctcgacgta cgcgctggcc aacctggacc acatccagga ggagaacctg cagcagaacg
     2281 cctacaaggt cggcaactac ctgtttcgcg gcctgaagga cctcgaggac cgcttcgacg
     2341 tggtcggcga ggtccggggg aaggggctga tgatcggcgt ggagctggtg aaggacgggg
     2401 agagcaaggc gccgagcccg gaggcggccg ggcgggtgat ggaggcctgc cgcgagcggg
     2461 gcctgctgat cggcaagggc gggctgtacg ggaacgtcct gcgggtgtcg cccccgctca
     2521 cgatcggtga ggacgacgcc gcccgcgcca tcgagacgct cgaggtggca ttcggagagg
     2581 cctcatcatg aggacgctga tcgggggcgg gacggtggtc acggcgagcg aggcgacggt
     2641 cgccgacgtg ctcgtccagg acgagcgagt cgccgcggtc ggagtggggc tcgacgcgca
     2701 ggccgacagg accatcgacg cctccggccg ctacgtgatg cccgggggcg tcgacgtcca
     2761 cacgcacttc gacctgccgt tcggcgggag cttctgctcc gacgacttcg acaccgggac
     2821 ccgcgccgcg gcgttcggcg ggaccaccac ctgcgtcgac ttcgcggtcc aggacgccgg
     2881 cgagtcgctg cgccagggcc tggatcgctg gttcgagaag ggccagaagg cgcagaccga
     2941 ctacggcctg cacatgatcg tgcgcgaggt gaacgacggg acgctgaagg agatggacga
     3001 gctggtgagc gagggcatca cctcgttcaa gctgttcatg gcctacccgg gagtgttcat
     3061 gctcgacgac gcttcgatct tccgggccat gcagcgcgcg gccgagagcg gggcgctgat
     3121 catgatgcac gccgagaacg gcgggccgat cgacgtcctg gtcaagcagc tgatcggcga
     3181 ggggaagacc gagccccggt accacggtgc atcgcggccg gcggcgatgg agggcgaggc
     3241 cgtgcaccgc gtgttcaagc tcgccgagct ggccgggacc cccgcctaca tcgtccacct
     3301 ctcctcgcgg gacgccctgg aggcggtccg cgaggcgcgc gaccgcggcg tggcggcgta
     3361 cgcggagacg tgccctcagt acctctacct ctcgagcgac gacatggcac gcccgggctt
     3421 cgagggcgcg aagtacgtct gctccccgcc gctgcgcccg aaggatcacc agcaggacct
     3481 gtggagcggg ctggccgagg gcgcgctgca gctcgtgtcc accgaccacg cgccgttcaa
     3541 ctacgtcggc cagaaggatc tcgggaggga cgacttcgcc aagatcccga acgggctccc
     3601 gagcgtggag gatcgcgtga cactgatgta ccagcgcgtc cacgccgggc agatgagccc
     3661 cagccgatgg gtggagctcg tggccacgaa cccggcgcgg atgttcggcc tctacccgcg
     3721 caagggcacc atcgcgcccg gcttcgacgc ggacatcgtg gtgttcgacc cgaaccgcga
     3781 gcgcgtcctc tcggcggaga ctcaccacat gaacgtggac tactcctgct acgaggggat
     3841 gaccgtctgg gggttgcccg aggtggtcct gcagcgcggg accgtgctcg tggagagcgg
     3901 cgagtggctc gggaagccgg gacagggacg gttcgtcgcg cgcagccggt tccaggagcc
     3961 ataggaggcg atggcatgat ggcgacccgg gtgcagagcc tgatcggcgg gcggttccgg
     4021 gaatcgagcg gcgaggacgt gccggtcgac ccggtgttcg acccggcgac cggcgagacg
     4081 atcgcgctcc tgccctactc gacgcccgag gagatcgacg aggcgatccg ggcggcgagc
     4141 gaggcgttcc cggagtggtc gaacgtcccg gtccccgacc gggcccaggt gatgttccgg
     4201 ttcaaggcgc tgctcgagga gcacttcgac gagctgtcgc ggctcgtggt ccgcgagaac
     4261 ggcaagacgc tccccgaggc gagcggcgag gtgcgccggg cgatcgaggt cgtggagctc
     4321 gcgtgcggcg cgccgtccat gctgatgggg acgaacctgg accagatcgc cgacggcatc
     4381 gacgaggagc tggtgcggtt cccggtgggc gtgtgcgccg ggatcacgcc gttcaacttc
     4441 ccggccatgg tgccgctctg gatgttcccg gtggccctcg tcgccgggaa cacgttcgtg
     4501 ctgaagccat cgcagcgcac gccgctgtcg gcggtccgcc tggccgagct gctgaccgag
     4561 gccggcctgc ccgacggcgt gttcaacgtg gtccacggcg cgaaggacgc ggtggacgcg
     4621 ctcctgacgc acccggacgt cgccgccgtg tccttcgtcg gctcggtcgg ggtggcccgg
     4681 tacatctaca cgacggccgc ggcgcacggg aagcgcgtcc aggcgctcgg aggagccaag
     4741 aaccacctga tcgtgatgga cgacgccgac ctggagcgaa gcgtgaccgc gctgatctcc
     4801 tccgccttcg gcaacgccgg gcagcggtgc ctggccggga gcgtcgcggt gggcgtcggg
     4861 tcggtcggcg aggccctggt ccgcgagctg cgccaccagg cgtcggcact gcggctgggc
     4921 cccggggagg atcccgccac cgacatggga ccggtgatcc gcgaggagcg gcgcaaggag
     4981 ctgatcggct acatcgagga ggggcaggag gccggcgcga ccctcgcgct ggacggccgc
     5041 ggcgccggtc cggcgcgggg cttcttcctc gggccgacca tcttcgacgg cgtgacgccc
     5101 gacatgcggc tgtggaagga ggaattgttc gggccggtgc tctcggtgct gcgcgcggcc
     5161 gacatcgacg aggcgctccg cgtgctgaac gccagcaccg acgggaacgc cgcctcgatc
     5221 ttcaccacgt cgggacacaa cgcgcgcgag ttcaaacggc gggccgaggc cgggatgctc
     5281 ggcgtcaaca tcggggtggc ggcgccgatg gcgttcttcc cgttcaccgg ctggaagagc
     5341 tcgttcttcg gcgacctcca cgccaccggc gcggacgcct tccgcttcta cacccggcag
     5401 aaggtggtca cgtcccgctg gtggctggcg ccgacgctcg atccgacccg gccggagggc
     5461 cgcggccact agctgatgga gcccgtctac cggcccgtgg ccgggctcgc ctacgcggtg
     5521 tgctggatca tgggctggga catccgcgtg cgcggcgccg aacacgtgcc gcgtcacggg
     5581 ccggcgatcg tcgcggccaa ccacgttggg ttcctcgact tcgtgttcat cgggatgggt
     5641 gcccgccgcg tacggcggtg ggtgcggttc atggccttcg aacgggcctt ccggcactgg
     5701 ctgggcgggc cgctgctgcg cgggatgcgg cacatcccgg tcgaccgcta cggcgacgcg
     5761 cgtgcggcgc tcgacctcgc catccgggcg ctgcgctccg gcgaggtcgt cgggctccac
     5821 ccggagtcgc ggatcaaccg gacgttcgtg ccgacggccg gcaagtccgg cgccgcgcgg
     5881 atggcgctgg agacggccgc gcccctgatc ccgtgcgcgg tgtgggggag ccagcggctg
     5941 tggacgcgcg ggcgccgcga gctgcggcga ggcgtgatgg tcaccgtcga cttcggcccg
     6001 tcgatcgcgt accggcccgg cgagccgccg gcggaggtca ccgaacggct gatgggggag
     6061 atccggcggc tcgccgggcg cgccgccgaa cgctacccgc agcggccgcg cgggccggcc
     6121 gaccggtggt ggctgcccgc gcacctcgga gggaccgcgc cgccggccga ctccttcgtc
     6181 atcggaccgc ccgggcctga tcgggcgcgt gacggaaggc actccttcga ggagaccgac
     6241 gtcacgctgg gatcgtgacg cccggcaccg accgccgtgc gcccgtgccc tatcgtcggc
     6301 ggcacgcgac ggacgagacg gaaggaagcc gacgatgatg cagccgggat cactcgcgaa
     6361 ctaccggatc caggacgacc tgcgccgggc agaggcgtac cggcgctcga agctcacccg
     6421 tgagggcgcg gcccgcgagc agcgggcgca cggccgatcg gtgggccggg ccatcctgag
     6481 cgccctgctc tggccgatcc gccactgagg cccgggtcgt cgcctcagga tcgccgggga
     6541 gcggggatgg aaccgacgta tcccgccagt ccccagtaga cgggggtgtc gtcgaactcg
     6601 aaggtcagcg cgtcgccgtc gaccgtgacg acaccgtcca cgccctcgac caggacgagt
     6661 tcccggcgca gcgcctggcc gccgagctgt acctcgactc cgggacgcgg agcatggagc
     6721 gcgggatcgt ctccgccggc cgcgaccccg agaccggcga acacctgcgc ccgagcctgg
     6781 agctggtccg gctgaccgtc ccgcggcgcg tctacacctc gcggcagctg gacatggtcg
     6841 ccggctccgt ggccaacgtg tacgagcggc gcgaggggac ggccggcctg gcgatgacct
     6901 acgagccccg ctacctgcgc ttcttccagg cgcgcttcac gccgctctga tccacgcgtt
     6961 tcggccttct ctcggcgtgg gatcatgcct gtacgggagg gaccgggtat ggagttcgga
     7021 cggttcacct tcgggtcgat cgagatcgac gggacgacgt acgagcacga cctggtgatc
     7081 gatcgcgggc gcgtccggaa gcggaagaag ggcccgtcga aggagctccg ctcgggttac
     7141 gggcacaccc cgctctccgc cgcggaggac atcccctgga cgtgccggcg gctggtcgtc
     7201 ggcagcggtg ccgacggcgc gctcccggtc atggaccagg tggccgatga ggcccgccgt
     7261 cgcggggtcg agctcgtggt gcagcccacc gcacgggccc tggagacgct gcgcgccgga
     7321 tccgacgaca ccaacgcgat cctgcacgtg acctgctgac cgctcagcgc ggtcgcgagg
     7381 atcgctccac gatgccgcgg gcgacgatgg cggcgtcctc ggcgatgccg aagaacgtcg
     7441 cggacttgcg cttgcgctgg aaatgaacgc cggcgaagta gagccccggg acgaccgtac
     7501 tggcgccgtc ggtctggatc gggaagccca tgtcgtcgaa cgccccgggg accgtgacca
     7561 ggtcgcggta gtcggggcgg aagccgccgg cgaacacgac cgcgccgaac cccgcgaggt
     7621 ctagctcctc gggcgcttcg ccgtcgaacg gcgccggctc ggggatgccc ggcatgggca
     7681 ggccgcgcgc ctcgacagcg gtgcggacca gcccagcgaa ctcgcggaag cggtcgtcgc
     7741 cccaggtgac gctggcgccg aggtcgggcg cgaagcgggc ccgccgcccc gtcggacccg
     7801 aggaagtgcc cgagcaggat gacgcccatg gcccgcaggg tccggtagtg caggtcgtgg
     7861 ccaccggcgc ggccggagct cagcaggttg gccgccaggc gagcatccgg cgacgggagg
     7921 gcggccaccg gctggtcggc gaagccggtc tggacgaccc accagacgat gtcgtggccg
     7981 tccagccgcc gggggagcca cggcgccctc ccgcacgaca gggacacctc acggccggcc
     8041 tcgtgcagct cctccgcgat ctggcagcct gactggccgc tgccgatcac aagcacccgg
     8101 ccatcgggga gcgacgccgg gtcgcggtag tcgtccacgt cgacctggaa caggccgggc
     8161 ggcagcgtct cggcgccggg cggccggtgc ggccgggggt aaccgccggt cgcgatcacc
     8221 accgagcggg ccccgatctc gccgtccggg gtccgcagga cgaagccgcc gtccaccggc
     8281 tcgaacgcgg tgaccgcaac gccctcgcgg accggcgcct cgaccgcggc agcgtagcgc
     8341 ccgaggtagg cgacgatctc gtcgcggggc atgaacccgt ccgggtcgtc gccgtcatag
     8401 gggtggccgg ggagctggac gctccagttc ggcgtgacca ggcagaagct gctccaccgc
     8461 ccccgccagc gctcgcccgt gcgcccccgc tcgagcacca cgtggtccac gccggacccg
     8521 gtcagctcgt ggctgacaga cagccctgcc tgcccggccc cgacgacggc cacgtcgacc
     8581 cgctcggcca cggccggaca tactaccgcc gggcgggggg cggctcccgt gccgcccccg
     8641 cccgg
//
LOCUS       DASPWO010000533         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1006400, whole genome shotgun sequence.
ACCESSION   DASPWO010000533 DASPWO010000000
VERSION     DASPWO010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006400"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VEO00_12245"
     CDS             complement(<1..344)
                     /locus_tag="VEO00_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58802.1"
                     /translation="MDVLLRLLGLGIVTGVSWFGLAGDWADPSLLEAALPVVLVAVGF
                     ALAFREDPYLGTAGYLTFGVVTLVIVTVWALVRGAPVTWQGWLRVVSTAGAGYLSLAV
                     PTSVLSRFLNLIR"
     gene            complement(377..>3356)
                     /locus_tag="VEO00_12250"
     CDS             complement(377..>3356)
                     /locus_tag="VEO00_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017611692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HYU58803.1"
                     /translation="APPATPATPTYADFFASLRFRWPWRPYQEVILERFEARDRAKRT
                     FHVVAPPGSGKTIIGLEIARRIGRPAVTFSPTTTIQEQWAEKVPQFRPEADSPEESSR
                     VLSWVSTEPAKLGALSSLTYQSLATQTQEREFLDRIGRQGWIDELVAGGRSPEGAAAY
                     LDEIGAKAPDVLQGGVSNRARARKRELLASGKANLRDLLHPNALDLLDRIVANGTGCV
                     ILDEAHHLLDYWAIILADLIRRLPDVLVVGLTATPPASADPGDMANYLVLVDGIDFEV
                     PTPAVVKAGSLAPYQDMVLITRPTDGERAFLRAQHDSLAGAIAHVFADPRFTDHIDRR
                     INRPPDPSGARTPWRTLLDEEFDLAIAGTRLLRASGRPLAEDVEIPDIPEPLGADDRL
                     VLVREWCYGFLRLSPDLADRSALADLRAALRALGLIMTETGWRPAPSPVDRVLSYSAS
                     KVAGMVEILRAESAALGDRLRAVVLTDFERSSATALRHLASVLDPESGGAVRAIRALA
                     ADPATGALDPVMLTARTVLVDADFAPRFLAEARAWLAERGLAATLEVRDAGNRLALIE
                     GSGGDWGPRHYVALVTDLFERGVTRCLVGTRGLLSEGWDSLTLNTLVDLTTAGTFASV
                     NQIRGRSIRLDPNDPRKVADNWDVVCLAPDLEEGDRDLRRLAKKHHHVWGLGPDGRIV
                     RGVGHVDHRVLLAVEGFRASLPVHAVNQRSLRRAAQRDRAYDAWDVGGPYRGESLSGT
                     VVAPDPAKVRTAFTWKKSLRALLNLVILNLVFYALVFAQGLPNVLGMDAPWWVRALMV
                     GVLVLMPFVVSAPLAWRYVRAAFIDLPVDSYLADFGRAVTDALHATGLGAARPEQVRA
                     TEGTDGVYEVALDGTDPATATAFAAAVRELFLPIVDQRYLVTREEAALSGSFYRPVWY
                     VLRGLFRVFRRRRRVYHPVPAIFGRKRETAEAFGAAWKRWVGGGELVYTRSPEGAAIL
                     LRERAAGRARTPSATVEEWV"
BASE COUNT          478 a         1227 c         1251 g          400 t
ORIGIN      
        1 cggatcaggt tcaggaagcg ggagagcacc gacgtcggca cggcgaggct caggtacccc
       61 gcaccggcgg tggagacgac gcggagccac ccctgccagg tgaccggggc gccgcggacg
      121 agcgcccaga ccgtgacgat cacgagcgtc accacgccga acgtgaggta gccggcggtg
      181 ccgaggtacg ggtcctcgcg gaacgccagg gcgaacccca ccgcgaccag caccaccggc
      241 agcgccgcct cgagcaggct ggggtcggcc cagtcgccgg ccagcccgaa ccaggacacc
      301 ccggtgacga tcccgaggcc gagcagcctc agtaggacgt ccacgcctga ttatgcggag
      361 gtggcggtcc ccgagctcag acccactcct ccacggtggc gctcggcgtg cgggcgcggc
      421 cggcggcgcg ctcgcggagc aggatcgcgg cgccctcggg cgagcgggtg tagacgagct
      481 cgccgccgcc cacccagcgc ttccaggcgg ccccgaaggc ctcggccgtc tcccgcttgc
      541 ggccgaagat cgcggggacg gggtggtaga cgcggcgccg gcggcggaac acccggaaga
      601 gcccgcgcag cacgtaccag accggccggt agaacgatcc cgacagcgcc gcctcctctc
      661 gcgtgaccag atagcgctgg tcgacgatcg gcaggaacag ctcgcggacg gcggcggcga
      721 acgccgtggc agtggccggg tcggtgccgt cgagggcgac ctcgtagacg ccgtcggttc
      781 cctcggtggc gcggacctgc tccggccgcg cggcgccgag gccggtggcg tgcagcgcgt
      841 ccgtcaccgc ccggccgaag tcggccaggt aggagtcgac cgggaggtcg atgaacgccg
      901 ccctgacgta gcgccaggcg agcggggcgg agacgacgaa gggcatgagg accagcacgc
      961 cgaccatcag cgcccggacc caccacggcg cgtccatccc gagcacgttc ggaagcccct
     1021 gcgcgaagac cagggcgtag aagacgaggt tcaggatcac gaggttcagc agcgcccgga
     1081 gcgacttctt ccacgtgaag gcggtgcgca ccttcgccgg gtcgggcgcg acgacggtgc
     1141 cggacagcga ctcgccgcgg taggggccgc cgacgtccca tgcgtcgtag gcgcggtccc
     1201 gttgggcggc ccggcggagc gaccgctggt tcacggcgtg gaccggcagg ctggcgcgga
     1261 acccctcgac ggccagcagc acgcggtggt cgacgtggcc gacgccgcgc acgatccgcc
     1321 cgtccggccc cagcccccag acgtggtggt gcttcttcgc caggcgccgc aggtcgcggt
     1381 cgccctcctc caggtcgggg gccaggcaca ccacgtccca gttgtcggcc accttgcgcg
     1441 ggtcgttcgg gtcgagccgg atcgagcgcc cgcggatctg gttcacggat gcgaacgtcc
     1501 ccgcagtggt caggtcgaca agcgtgttca gcgtcaggct gtcccacccc tccgacagca
     1561 gcccgcgcgt cccgaccagg cagcgcgtca cgccgcgctc gaacaggtcg gtgacgagag
     1621 cgacgtagtg gcgcggcccc cagtcgccgc cgctgccctc gatcagcgcg aggcggttgc
     1681 ccgcatcgcg gacctcgagc gtcgccgcca ggccgcgttc cgccagccag gccctggcct
     1741 cggccaggaa ccgcggcgcg aagtcggcgt cgaccagcac cgtgcgcgcg gtgagcatca
     1801 cggggtcgag cgcgccggtc gccgggtcgg cggccagcgc ccggatggcc cggaccgcac
     1861 cgccagactc cgggtcgagg acgctggcca ggtgccgcag cgcggtggcg gaggagcgct
     1921 cgaagtcggt cagcaccacc gcgcggagcc ggtccccgag cgcggccgac tccgcccgca
     1981 ggatctccac catgccggcc accttgctcg ccgagtacga gagcacccgg tccaccggcg
     2041 agggcgccgg ccgccagccc gtctcggtca tgatcaggcc gagcgcccgc agcgccgcgc
     2101 gcaggtccgc gagcgcggag cggtcggcga gatccggcga gaggcgcagg aacccgtagc
     2161 accactcccg gaccaggacc aggcggtcgt cggcgccgag cggctccggg atgtcgggta
     2221 tctcgacgtc ctcggccagc ggccgtccgc tcgcgcgcag caggcgcgtc ccggcgatcg
     2281 ccaggtcgaa ctcctcgtcg agcagcgtcc gccacggcgt gcgcgcgccg ctcgggtcgg
     2341 ggggtcggtt gatgcgccgg tcgatgtggt cggtgaagcg cgggtcggcg aacacgtggg
     2401 cgatcgcgcc ggcgaggctg tcgtgctggg cccgcaggaa cgcccgttcc ccgtcggtcg
     2461 gccgggtgat cagcaccatg tcctggtacg gcgccaggct cccggccttc acgacggccg
     2521 gcgtgggcac ctcgaaatcg atcccgtcga cgagcaccag gtagttcgcc atgtcgcccg
     2581 gatctgcaga tgcgggcggc gtcgcggtca gccccacgac cagcacgtct ggcagccggc
     2641 ggatcaggtc ggccaggatg atcgcccagt agtcgagcag gtggtgcgcc tcgtcgagga
     2701 tcacgcagcc ggtcccgttc gccacgatcc ggtcgagcag gtccagcgcg ttggggtgca
     2761 gcaggtcgcg caggttcgcc ttcccggacg ccagcagctc gcgcttgcgc gcccgggccc
     2821 ggttcgagac gccgccctgg agcacgtcgg gggccttcgc cccgatctcg tccaggtagg
     2881 ccgcggcccc ctccggcgag cggccgcccg cgaccagctc gtcgatccag ccctgccgcc
     2941 cgatccggtc gaggaactcg cgctcctgcg tctgggtggc caggctctgg tacgtcagcg
     3001 acgacagggc gccgagcttc gccggctcgg tcgacaccca ggagagcacg cgggaggact
     3061 cctcggggga gtcggcctcc ggtcggaact gcgggacctt ctcggcccac tgctcctgga
     3121 tcgtggtggt cggcgagaac gtgacggcag gccgtccgat ccgccgcgcg atctccaggc
     3181 cgatgatcgt cttccccgac ccgggcggcg cgacgacgtg gaaggtgcgc ttcgcccggt
     3241 cgcgggcctc gaaccgctcg aggatcacct cctggtacgg ccgccagggc cagcggaacc
     3301 gcagcgaggc gaagaagtcg gcgtacgtcg gggtggcggg ggtcgccggc ggcgcc
//
LOCUS       DASPWO010000534         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1006410, whole genome shotgun sequence.
ACCESSION   DASPWO010000534 DASPWO010000000
VERSION     DASPWO010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006410"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VEO00_12255"
     CDS             complement(<1..300)
                     /locus_tag="VEO00_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HYU58804.1"
                     /translation="MNPLLYVCLDGLGDDPVPALGGRTPLEAATTPFLDSLAMRGAQG
                     LVQTVGEGIAPESDIAVFAILGYDPREEHPGRGVVEAVGTAMDFRDGDLAFRVNFA"
     gene            complement(310..1080)
                     /gene="tpiA"
                     /locus_tag="VEO00_12260"
     CDS             complement(310..1080)
                     /gene="tpiA"
                     /locus_tag="VEO00_12260"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HYU58805.1"
                     /translation="MAERKPILAANWKMHKTHLEAIQAVQKLSYLLDARRAEAVEVVI
                     CPPFTALRSVQTVLEADRLPYGLGAQDTHWEDAGAFTGAVSPAMLAALKVGYVVVGHS
                     ERRELFGETDETVAKKVRAVFAHDMAPILCVGETLDEREGGRTEEKVVGQVKAALAGL
                     ASEQVASLIVAYEPIWAIGTGRNATAADAGEVVRTIRQTVAGLASADAAASLRVQYGG
                     SVKPGNIREFMAHPEIDGALVGGASLDPEDFALIVQYE"
     gene            complement(1095..2279)
                     /locus_tag="VEO00_12265"
     CDS             complement(1095..2279)
                     /locus_tag="VEO00_12265"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HYU58806.1"
                     /translation="MRTIEDLGDLAGRRVLVRVDFNVPLADGRVADDFRIRAALPTIS
                     ELLDDGASLVLASHLGRPKGVDEALRLAPVAARLGELLGRDVTALPEVVGDNVTGAVR
                     AVPRRSVVMLENLRFDPGETANDPAFAAALASLGEAFVDDAFGAAHRAHASVVGVAER
                     LPSAAGRLLQREVEVLSELRDGPERPYVAILGGAKVSDKLGVLRALVERVDALLVGGA
                     MAFTLLLARGGEVGDSLVEPDRLDDVREALAAADARGVPVILPEDVVAAPEADAGAPR
                     QTVPADRIPAGMKGLDIGPRSVEEFVRTLADARTILWNGPMGVFELEPFAAGTVGVAK
                     GVAGAEAFTVVGGGDSLLALQRTGLADRIDHLSTGGGASLEFIEGHTLPGVAVLQSDS
                     GS"
     gene            complement(2292..3305)
                     /gene="gap"
                     /locus_tag="VEO00_12270"
     CDS             complement(2292..3305)
                     /gene="gap"
                     /locus_tag="VEO00_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017582648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HYU58807.1"
                     /translation="MGATGVKLGINGFGRIGRNFLRAAKKLGADFDFVAVNDITDAGT
                     LAHLLRYDSVLGTFDAEVSVSDNGIVVDGDELRVTAERDPANLPWKELGAEIVLESTG
                     LFTSREQAEKHVAAGAQKVVISAPAKGEDITIVMGVNDGDYDPAAHNIVSNASCTTNC
                     VVPLASVLDEAFGIDRALMTTVHAYTNDQQLLDLPHKDLRRARAAALNIIPTSTGAAK
                     ATSIAIPKLKGKMDGMSLRVPVPDGSITDLVCLLDRDASVDELNAAYREAAEGRLRGV
                     LRYTEDPIVSSDIVGDPHSCVFDAQSTMAIGNFVKVFGWYDNEWGYSNRLVDLIQLIA
                     SKL"
     gene            complement(3370..>3564)
                     /locus_tag="VEO00_12275"
     CDS             complement(3370..>3564)
                     /locus_tag="VEO00_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58808.1"
                     /translation="TTTTTTTTTKPPGGGCTIPIQCTTQSHAPAAVTAGPSTNAIAIG
                     VALLALLLGASIAARARHPA"
BASE COUNT          490 a         1291 c         1275 g          508 t
ORIGIN      
        1 ggcgaagttg acccggaacg cgaggtcgcc gtcgcggaag tccatggcgg tgccgaccgc
       61 ctcgaccacg cccctgccgg ggtgctcctc gcgcgggtcg tacccgagga tggcgaacac
      121 ggcgatgtcc gactccgggg cgatcccctc cccgacggtc tgcaccaggc cctgcgcccc
      181 gcgcatggcg agcgagtcga ggaacggcgt ggtggccgcc tcgagcggcg tgcggccgcc
      241 gagtgccggg acgggatcgt cgcccagacc gtccagacag acgtagagga ggggattcac
      301 ggcgcgaacc tactcatact ggacgatcag cgcgaagtcc tccgggtcga ggctggcccc
      361 gccgacgagt gccccgtcga tctcgggatg ggccatgaac tcgcggatgt tgccgggctt
      421 cacgctgccg ccgtactgca cccgcaggga cgcggcggcg tcggccgatg ccaggccggc
      481 gacggtctgg cggatcgtcc tcaccacctc accggcgtcg gccgcggtgg cgttgcggcc
      541 ggtgccgatc gcccagatcg gctcgtaggc gacgatcagg gaggcgacct gctcggacgc
      601 cagcccggcg agcgcggcct tcacctgtcc gaccaccttc tcctccgtcc ggccgccctc
      661 ccgctcgtcg agcgtttcgc cgacgcagag gatcggcgcc atgtcgtggg cgaacaccgc
      721 ccgaaccttc ttcgccacgg tctcgtcggt ctcgccgaac agctcccgcc gctcggagtg
      781 cccgacgacc acgtagccca ccttgagcgc ggcgagcatg gcgggcgaga ccgcgccggt
      841 gaacgcgccg gcgtcctccc aatgcgtgtc ctgcgcgccg agcccgtacg ggaggcggtc
      901 ggcctccagc accgtctgga cgctgcgcag ggccgtgaac ggagggcaga tcaccacctc
      961 gacggcttcg gcccggcgcg cgtccagcag gtacgagagc ttctgcacgg cctggatggc
     1021 ctcgaggtgc gtcttgtgca tcttccagtt cgccgccagg atcggcttcc gctcggccat
     1081 cgcgcctcct cgtcctagga cccggaatcg gactgcagga ccgcgacgcc gggaagggtg
     1141 tgcccctcga tgaactcgag cgacgctccg ccgccggtcg acaggtggtc gatccgatcc
     1201 gccaggcccg tgcgctgcag ggcgagcagg ctgtcgccac ccccgacgac ggtgaacgcc
     1261 tcggcgccgg cgaccccttt cgccacgccg accgtccccg cggcgaacgg ctccagctcg
     1321 aatacgccca tcgggccgtt ccagaggatc gtccgggcgt cggccagcgt gcgcacgaac
     1381 tcctcgacgg accgcggccc gatgtccagg cctttcatcc cggcggggat gcggtcggcc
     1441 ggcacggtct ggcggggcgc gccggcatcg gcctccgggg cggcgacgac gtcctccggc
     1501 aggatcaccg ggacgccgcg cgcgtcggcg gcggccaggg cttcgcgaac gtcgtccagc
     1561 cggtccggct cgaccaggga gtcgccgacc tcgccgccgc gagccagcag cagcgtgaag
     1621 gccatcgccc ccccgacgag cagcgcatcg acccgctcga ccagtgcccg cagcaccccg
     1681 agcttgtcgg agaccttcgc cccgccgagg atcgccacgt agggccgctc cgggccgtcg
     1741 cgcagctccg acagcacctc cacctcgcgc tgaagcagcc gtcccgcggc cgacgggagc
     1801 cgctcggcca cgccgaccac gctggcgtgg gcgcggtgcg ccgcgccgaa cgcgtcgtcg
     1861 acgaaggcct cgcccagcga ggccagggcc gcggcgaagg ccgggtcgtt cgcggtctca
     1921 ccggggtcga agcggaggtt ctccagcatc accaccgagc ggcggggcac ggcccgcacc
     1981 gccccggtga cgttgtcgcc gaccacctcg gggagggccg tcacgtcgcg gccgagcagc
     2041 tcgccgaggc gggcggcgac cggagccagc cgcagcgcct cgtccacgcc cttcgggcgg
     2101 ccgagatgcg acgccagcac gagcgacgcg ccgtcgtcga gcagttcgga gatcgtcggg
     2161 agggcggcgc ggatgcggaa gtcgtcggcc acccgcccgt cggcgagcgg gacgttgaag
     2221 tcgacgcgga cgagcacccg ccgtccggcg agatcgccca ggtcctcgat cgtgcgcagg
     2281 cgcggcacgc gctacagctt ggaggcgatc agctggatca ggtcgacgag ccggttcgag
     2341 tacccccact cgttgtcgta ccagccgaag accttcacga agttcccgat ggccatcgtg
     2401 ctctgcgcgt cgaagacgca cgagtgcgga tcgccgacga tgtccgagga gacgatcggg
     2461 tcctccgtgt agcgcagcac cccgcggagg cgcccttcgg cggcctcgcg gtaggccgcg
     2521 ttgagctcgt cgaccgaggc atcgcggtcg agcaggcaca ccaggtcggt gatcgagccg
     2581 tccgggaccg gcacccgcaa cgacatcccg tccatcttcc ccttcagctt ggggatggcg
     2641 atcgaggtgg ccttcgccgc gccggtcgag gtggggatga tgttcagcgc ggccgcccgc
     2701 gcccggcgca ggtccttgtg cggcaggtcg agcagctgct ggtcgttcgt gtaggcgtgg
     2761 acggtggtca tcagggcgcg gtcgatgccg aacgcctcgt cgagcacgct ggccagcggc
     2821 acgacgcagt tcgtggtgca cgacgcgttc gagacgatgt tgtgggcggc agggtcgtag
     2881 tccccgtcgt tgacgcccat gacgatcgtg atgtcctcgc ccttcgccgg tgcgctgatc
     2941 acgaccttct gcgcgccggc ggcgacgtgc ttctcggcct gctcgcgcga ggtgaacagg
     3001 ccggtggact cgagcacgat ctccgctccg agctccttcc acggcaggtt ggccgggtct
     3061 cgctcggcgg tgacccgcag ctcgtcgcca tccaccacga tgccgttgtc gctcaccgag
     3121 acctcggcgt cgaacgtccc gagcaccgag tcgtagcgca gcagatgcgc cagcgtgccg
     3181 gcgtcggtga tgtcgttgac ggcgacgaag tcgaagtccg ctccgagctt cttcgccgcg
     3241 cgcaggaagt tccgtccgat gcggccgaac ccgttgatcc cgagtttcac gcccgtggca
     3301 cccatggcag gtcctcccct tcggtcgacc ggttcatcct agttggagcg ggaggaggcg
     3361 gccgccgcgt caggccgggt ggcgcgcccg cgccgcgatg gaggcgccca gcagcagggc
     3421 gagcagcgcc acgccgatcg cgatcgcgtt ggtggagggc ccggcggtga cggcggccgg
     3481 ggcatgcgac tgggtcgtgc actggatggg aatggtgcat ccccctcccg gcggcttggt
     3541 cgtggtcgtg gtggtcgtcg tggt
//
LOCUS       DASPWO010000535         3643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1006569, whole genome shotgun sequence.
ACCESSION   DASPWO010000535 DASPWO010000000
VERSION     DASPWO010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006569"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VEO00_12280"
     CDS             <1..263
                     /locus_tag="VEO00_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYU58809.1"
                     /translation="HDLTSFVGSIRERSLLDRVFRDPDAMQADVAQVMGPPFPAVGEH
                     EPVEVAFEQLQSGPAVIVARGSEAIGVVTRSDLLEFLAERDR"
     gene            278..928
                     /locus_tag="VEO00_12285"
     CDS             278..928
                     /locus_tag="VEO00_12285"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HYU58810.1"
                     /translation="MRSLGIDIGASRKGFDAVLLDDALVPLRTARRLGAADVGALIDT
                     ARPDVVAIDSPPAWGLRGGSRATERELRARGIHAFATPSDLRKAESRFYDWMRCGFEA
                     FAVAAARGYARYRSGRVRGTAIEIFPHASAVVLAGRLPPAGARKRPFREDVLRAAGVD
                     TRGLRSIDQIDACLAALTGLLALRGTRCAPGDPAEGVIVLPATSFPAPPFHRPRSG"
     gene            940..2064
                     /locus_tag="VEO00_12290"
     CDS             940..2064
                     /locus_tag="VEO00_12290"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HYU58811.1"
                     /translation="MRFRTRAIHAGQDPEPAYGAVNVPIYQTSTYAQNAVGEHKGYDY
                     ARTRNPTRTALETCLAALEDAAHGLCFASGMAAEATFLQTLRPGDHVVLGDDVYGGTY
                     RLIARVLADWDLQFDTVDLTDGEAVRKALREETRAVWVETPTNPLLKVIDIAAVAELA
                     HEAGARVVVDNTFATPALQRPLERGADVVVHSTTKYLGGHSDVVGGALLTSDDALHDR
                     LAFLQNAVGAVPGPLDCYLVLRGVKTLALRMVAHCAGAARIAEFLAAHPRVTAVHYPG
                     LESHPQHELAARQMDGFGGMVSFELESEQAALAVPARTRLFFLAESLGGVESLIEHPG
                     RMTHASVAGSPLEVPPTLVRLSVGLEDPDDLLEDLERALS"
     gene            2113..2730
                     /locus_tag="VEO00_12295"
     CDS             2113..2730
                     /locus_tag="VEO00_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYU58812.1"
                     /translation="MKQAVRKRVAKPAEERRVDLMDAALRVFLEKGAASTTVADITDA
                     AGVAKGTFYLYFDSKEALLGALKERFVDDMIAHASQFAERVGREDWWILAEATAEGWV
                     DFALEHRAQIRLFAQEGVTVETFEPFAECEAKLHGLFVAGIEAGTRAGAFHVTDPELT
                     AHFVSKALEGTVEHAILFGAAIDRDALVTGAKELVRKVLGPSVAS"
     gene            complement(2737..3207)
                     /locus_tag="VEO00_12300"
     CDS             complement(2737..3207)
                     /locus_tag="VEO00_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006036429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HYU58813.1"
                     /translation="MSEHDDDEDEDGPVYSPSDDELRRIYADTRTIAVVGASGNPSKA
                     AYSIPRYLQSQGYRIIPVNPSGGTIHGEPARAALSEIDEPIDVVDVFRPAAETPGVAR
                     AAVAAGAKVLWLQEGIYNDEARAIGEDAGLTVIMGKCMGATHWTLIGEGSRGGV"
     gene            complement(3222..>3643)
                     /locus_tag="VEO00_12305"
     CDS             complement(3222..>3643)
                     /locus_tag="VEO00_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58814.1"
                     /translation="GFRYNARVLVDHIAERRFGWAPPRPAVPPDALARAITDEMARSP
                     ALWNQKAYLARVFEAVADGVVDAGLAPLQAFVDSGGPDAVAATVETTDAGEIRPVLYV
                     RRRGSVEEHPLLPHPLQDFGGPEYEQEVAMLLKGLLS"
BASE COUNT          475 a         1370 c         1289 g          509 t
ORIGIN      
        1 accacgacct gacgagcttc gtcggcagca tccgcgagcg gagcctgctc gaccgcgtgt
       61 tccgcgatcc cgacgcgatg caggccgacg tggcgcaggt gatggggccg ccgttccccg
      121 ccgtcggcga gcacgagccg gtcgaggtgg ccttcgagca gctgcagtcc gggccggccg
      181 tgatcgtggc ccgcggctcc gaggccatcg gcgtcgtcac ccgctcggac ctgctcgagt
      241 tcctcgccga acgggaccgc tagcggccgc cagctcggtg cggtcgctcg gcatcgacat
      301 cggcgcgtca cggaagggct tcgacgccgt cctactcgac gacgcgctgg tcccgctccg
      361 gacggcccga cggctcggcg ccgcggacgt cggcgcgctg atcgacaccg cgaggccgga
      421 cgtcgtggcg atcgactcgc cgccggcgtg gggcctgcgc ggcggctccc gggcgaccga
      481 gcgcgagctg cgggcgcgcg ggatccacgc gttcgcgaca ccgtcggacc tgcgcaaggc
      541 cgagagccgt ttctacgatt ggatgcgctg cgggttcgag gccttcgcgg tcgccgccgc
      601 ccgcggatac gcgcgctacc ggagcggccg cgtgcgcggc acggcgatcg agatcttccc
      661 ccacgccagc gcggtggtcc tcgccggccg gctgcccccg gccggggcgc gcaagcgccc
      721 gttccgcgag gacgtgcttc gagcggcggg tgtcgacacg cgggggctgc ggtcgatcga
      781 tcagatcgac gcctgcctgg ccgcgctgac cggcctgctc gcgctccgcg ggacgcgctg
      841 cgctccgggc gaccccgccg agggcgtcat cgtgcttccg gccacctcct tcccggcccc
      901 gccgttccac cggccccgtt ccggctaggc tgggccccga tgcgtttccg gaccagggcg
      961 atccacgccg ggcaggatcc cgagcccgcc tacggcgcgg tgaacgtgcc gatctaccag
     1021 acctcgacct acgcgcagaa cgccgtcggc gagcacaagg ggtacgacta cgcccgaacc
     1081 aggaacccca cgcgcacggc gctcgagacc tgcctggccg cgctggagga cgccgcgcac
     1141 ggcctgtgct tcgccagcgg gatggccgcc gaggccacgt tcctgcagac gctccgcccc
     1201 ggcgaccacg tggtgctcgg ggacgacgtc tacggcggca cctaccgcct gatcgctcgc
     1261 gtcctcgcgg actgggacct gcagttcgac accgtcgacc tcaccgacgg ggaggccgtg
     1321 cgcaaggccc tgcgcgagga gacgcgcgcg gtgtgggtcg agaccccgac caacccgctg
     1381 ctgaaggtga tcgacatcgc cgccgtcgcc gagctggccc acgaggccgg cgcgcgcgtc
     1441 gtcgtcgaca acacgttcgc cacgccggcg ctgcagcggc cgctcgagcg cggcgccgac
     1501 gtcgtcgtcc actcgaccac gaagtacctg ggcgggcact ccgacgttgt cggcggcgcc
     1561 ctcctcacct ccgacgacgc cctgcacgac cggctggcgt tccttcagaa cgcggtcggc
     1621 gcggtccccg ggccgctcga ctgctacctg gtgcttcgcg gggtgaagac gctcgcgctg
     1681 cgcatggtgg cgcactgcgc cggggcggcg cggatcgccg agttcctcgc cgcccaccca
     1741 cgggtcaccg ccgtccacta cccggggctg gagtcgcacc cgcagcacga gctcgccgcg
     1801 cggcagatgg acggcttcgg cgggatggtg tcgttcgagc tggagagcga gcaggccgcc
     1861 ctggcggtcc cggcgcggac gcggctcttc ttcctggcgg agtcgctcgg cggcgtcgag
     1921 tcgctgatcg agcaccccgg ccggatgacc cacgccagcg tggccggctc gccgctcgag
     1981 gtgccaccga cgctcgtccg gctgtcggtc ggcctcgagg accccgacga cctgctcgag
     2041 gacctggagc gggcgctttc ctaggacccc cgaacaccgg cggctatact gactgaccgg
     2101 gggtcagtgg agatgaagca ggccgttcgg aagcgggtcg ccaagccggc ggaggagcgc
     2161 cgcgtcgacc tgatggacgc cgcgctccgg gtgttcctgg agaagggcgc cgccagcacg
     2221 acggtggcgg acatcaccga cgccgccggc gtggcgaagg ggaccttcta cctctacttc
     2281 gattcgaagg aggcgctcct cggcgcgctg aaggagcgct tcgtcgacga catgatcgcc
     2341 cacgcctcgc agttcgccga gcgggtcggc cgcgaggact ggtggatcct ggccgaggcc
     2401 accgccgagg gctgggtgga cttcgccctg gagcaccgcg cccagatccg gctgttcgcc
     2461 caggaggggg tcacggtcga gacgttcgag ccgttcgccg agtgcgaggc caagctgcac
     2521 gggctgttcg tcgccgggat cgaggccggc acccgtgccg gcgcgttcca cgtcaccgac
     2581 cccgagctca ccgctcactt cgtctcgaag gcgctcgagg ggaccgtcga gcacgcaatc
     2641 ctgttcggcg cggcgatcga ccgcgacgcc ctggtgaccg gcgccaagga gctggtgcgc
     2701 aaggtcctcg ggccctccgt ggcttcctga cctcgctcag acgccgccgc gcgacccctc
     2761 cccgatgagc gtccagtgcg tcgcgcccat gcacttgccc atgatcacgg tcagcccggc
     2821 atcctccccg atcgcccggg cctcgtcgtt gtagatcccc tcctgcagcc agagcacctt
     2881 cgcccccgcg gccaccgccg cgcgcgccac gccgggcgtc tcggcggccg gccggaagac
     2941 gtcgacgacg tcgatcggct cgtcgatctc ggagagcgct gcgcgtgccg gctcgccgtg
     3001 gatcgtcccg ccggacgggt tcaccgggat gatccggtag ccctgcgact gcaggtagcg
     3061 cgggatgctg taggcggcct tcgacgggtt ccccgacgcc ccgaccacgg cgatcgtgcg
     3121 ggtgtccgcg tagatcctcc gcagctcgtc gtcggacggg ctgtagaccg gaccgtcctc
     3181 gtcctcgtca tcgtcgtgct cgctcatcga tcctcccttc gctaggacag cagacccttc
     3241 agcagcatcg ccacctcctg ctcgtactcc gggccgccga agtcctgcaa cggatggggc
     3301 agcagcggat gctcctcgac cgagccgcgg cggcggacgt agaggacggg gcggatctcc
     3361 ccagcgtcgg tggtctcgac cgtcgcggcc acggcgtccg gaccgcccga gtcgacgaag
     3421 gcctggagcg gcgccagccc ggcgtcgacc acgccgtcgg ccacggcctc gaacacgcgg
     3481 gccagatagg ccttctggtt ccagagcgcc gggctcctcg ccatctcgtc ggtgatcgcg
     3541 cgcgcgagcg cgtcgggcgg caccgccggg cgcggaggcg cccaaccgaa tcgccgctcg
     3601 gcgatgtggt ccaccagcac gcgggcgttg taacggaacc cgt
//
LOCUS       DASPWO010000536         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1006627, whole genome shotgun sequence.
ACCESSION   DASPWO010000536 DASPWO010000000
VERSION     DASPWO010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006627"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VEO00_12310"
     CDS             complement(<1..798)
                     /locus_tag="VEO00_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58815.1"
                     /translation="MPDVRQLLERGVADYRPAPDALQRTLRRVRRRQLRRRSLAAFLA
                     FVLTGASIWALTRAFLGSGPAAPAVQPRVTATIPTFPPAGDVEVAFGRVWVVTVKGLY
                     GIDAATGEVALVGDQELDPDFDKVAAGHDGLWVSHGNQVTLVDPEDGRRIRSVSMGST
                     VTDVVVAGGYVWGTRARDGRGDLLRVTEDAAEAEPIQGPPPCVCVELGEGPARAVAGG
                     RFVWVSNLSGERYVSKVDPDRAEVVGNVGLPGPVAFGFRWLWAVGDDG"
     gene            complement(791..1309)
                     /locus_tag="VEO00_12315"
     CDS             complement(791..1309)
                     /locus_tag="VEO00_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYU58816.1"
                     /translation="MAESAEVEAAAVSVGPLAFEDFFEREYANLARALLLLTGERAEA
                     EDLAQEALARVFERWDRVAAMESPSGYLYRTALNLNRKRLRRQGVRRRHRTESRPEPA
                     PEDVAETRDAILRALRSLPPAQREALVLVEWLGLEAEEAGAVLGIAAASVRGRLHRAR
                     QSLRARFGGPDA"
     gene            complement(1380..2018)
                     /locus_tag="VEO00_12320"
     CDS             complement(1380..2018)
                     /locus_tag="VEO00_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006347979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYU58817.1"
                     /translation="MLVDDHEVVRGGIRSLLEATEDIVVTAEAGTVRDAVDEAERSRP
                     DIVVMDVRLADGSGIEATREIRARRPATQVLMLTSFADDEALFASIMAGASGYVLKQV
                     RSGELVRAIRTVGSGQSLLDPAMTSAVLDRLRKGKHLMRDEKLARLSPQEERILALVA
                     DGRTNREIGEELHLAEKTVKNYVSSILSKLEVARRAEAAAYLARHTTTPGQV"
     gene            2315..2782
                     /locus_tag="VEO00_12325"
     CDS             2315..2782
                     /locus_tag="VEO00_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58818.1"
                     /translation="MSSDRDRRETEAEPTVLVVAQDREERGRVGAWLEEAGFSVMMCS
                     GPTEPDYVCLAGRSRTCPIAEEADAVVLDLWLAGDTVMRGMSGNELLTYYLSRGKPVV
                     TLSHGRETATLQADDQVTPIAYPPEREKVLAAVAEAVWPYAPHVPPLIDRRAR"
     gene            2779..3081
                     /locus_tag="VEO00_12330"
     CDS             2779..3081
                     /locus_tag="VEO00_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58819.1"
                     /translation="MSTITDVRERIEARLGIVYDIMEPAERSLVPLRPRERSHSLPAG
                     SPISLAAYVIRKAETDRIPITGSAGTVIGTVTLAAVEDAVACASWTLHHPAARPAH"
     gene            complement(3107..>3467)
                     /locus_tag="VEO00_12335"
     CDS             complement(3107..>3467)
                     /locus_tag="VEO00_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58820.1"
                     /translation="LRCSRVRGGIRPQLPAAQLPEAAALLAAGIGSLVLGILTTIAEA
                     SASFADALALNDRVGPLSGKTIYAVAAWVVAWIILGIALRRKDPSPGGMFTLTAILVL
                     LGFLGTLPTFFEKFAPE"
BASE COUNT          498 a         1290 c         1236 g          443 t
ORIGIN      
        1 cccgtcgtcg ccgacggccc acagccagcg gaacccgaac gcgaccggcc cgggcagtcc
       61 cacgttcccg accacctccg cccgatccgg gtccaccttc gagacgtatc gctccccgct
      121 caagttcgag acccagacga agcggccgcc cgccacggcc cgagcggggc cttcacccaa
      181 ctcgacgcac acgcagggcg gcggtccctg gatgggttcg gcctccgccg cgtcctccgt
      241 cacgcggagc agatccccgc gcccatctcg agcccgcgtt ccccacacgt acccaccggc
      301 gaccacgaca tcggtaaccg tcgaacccat cgagaccgac cggatgcgcc ggccatcctc
      361 gggatccacg agcgtgacct ggttcccatg gctcacccac aggccgtcgt ggccggccgc
      421 aaccttgtcg aagtcagggt cgagttcttg gtcgccaacg agagccacct cacccgtggc
      481 ggcgtcgatc ccgtacaggc ccttcacagt gaccacccac acccggccaa aggccacctc
      541 gacgtcgccg gcgggcggaa aggtcgggat cgtggcggtc acgcgcggct ggacggcggg
      601 tgcggctggt ccggacccga ggaaggcccg ggtcagggcc cagatcgagg cgccggtcag
      661 gacgaaggcc aggaacgcgg ccagggagcg ccggcggagc tggcggcggc ggacgcggcg
      721 gagggttcgc tgcagggcgt cgggtgcggg ccggtagtcg gccacgccgc gctcgagcag
      781 ttgccggacg tcaggcatcg ggacctccga acctcgcgcg gagcgactgg cgggcccggt
      841 ggaggcggcc gcggaccgag gcggccgcga tgccgagcac cgcgccggcc tcctccgcct
      901 ccaggccgag ccactcgacg agcaccagcg cctcgcgctg cgccggcggc agcgaccgca
      961 gcgcgcgcag gatcgcgtcg cgggtctcgg ccacgtcctc cggggccggc tccgggcgtg
     1021 actccgtccg atggcgcctg cgaacgccct gccggcgcag gcgcttgcgg ttcaggttca
     1081 gcgcggtgcg gtacaggtag ccgctcgggg actccatcgc cgccacgcga tcccatcgct
     1141 cgaacacccg ggccagcgcc tcctgcgcca ggtcctcggc ctcggcccgc tcgccggtga
     1201 gcagcagcag cgcccgcgcc aggttcgcgt attcgcgctc gaagaagtcc tcgaacgcga
     1261 gcggaccgac ggacaccgcc gcggcctcca cctcggcgga ctcggccatc accgaagcat
     1321 gaacccccga ccgcgctgcc acgttcgggt cgacggccca gaggccggag aagggcggat
     1381 cagacctggc cgggggtggt ggtgtggcgg gcgaggtagg ccgcggcctc cgcccgccgg
     1441 gcgacctcga gcttggagag gatgctcgag acgtagttct tgacggtctt ctcggccagg
     1501 tggagctcct cgccgatctc gcgattggtc cgcccgtcgg cgacgagcgc caggatgcgc
     1561 tcctcctgcg gcgacagccg ggcgagcttc tcgtcccgca tcaggtgctt gcccttgcgc
     1621 agccggtcga gcacggcgct ggtcatcgcc gggtcgagca ggctctgccc ggacccgacc
     1681 gtgcggatcg cccgcaccag ctcaccgctt cgcacctgct tcagcacgta gccggaggcg
     1741 ccggccatga tcgaggcgaa cagcgcctcg tcgtcggcga acgaggtcag catcagcacc
     1801 tgcgtcgccg ggcgccgggc gcggatctct cgcgtcgcct cgatcccgct gccgtcggcc
     1861 agccggacgt ccatcaccac gatgtcgggg cggctgcgct cggcctcgtc gaccgcgtcg
     1921 cgcaccgtcc cggcctccgc agtgaccacg atgtcctcgg tggcctccag cagcgagcgg
     1981 atcccgccgc ggacgacctc gtggtcgtcc accagcatca cgcgcagcgg ctgcacgctc
     2041 ggcgagcgcg gctggccggg ctctgcggcc ttcgcccggg ggggcgtcgt gcgcggatcg
     2101 gcgcccctgc cctgggggct ccgcttcggt gacccccctt tcccggccct cgatgctggg
     2161 ctcatcgtct ggattctccc atcagaccgc cggattgccc gtcgtcggcg ctcgcgagcg
     2221 gtggcgaagg tccccccgga gacgggacct gcgccccggg ccggcgcccg gggaacgggc
     2281 caccctttcg acaggacggg tggaaggggg acacatgagc agcgaccgtg accggcggga
     2341 aaccgaggcg gagcccaccg tcttggtcgt cgcgcaggat cgcgaggagc gggggcgcgt
     2401 gggagcctgg ctggaggaag ccgggttctc ggtgatgatg tgctctgggc ccacggagcc
     2461 cgactacgtc tgcctggccg gacgaagccg cacctgtccg atcgcggagg aggcggacgc
     2521 ggtggtgctc gacctgtggc tggccggcga cacggtgatg cgcgggatgt ccgggaacga
     2581 gctgctgacc tactacctgt cgcgcggcaa gcccgtggtc accctgtccc acggccgcga
     2641 gacggcgacg ctgcaggccg acgatcaggt cacgccgatc gcctacccgc cggagcggga
     2701 gaaggtgctc gcggccgtgg ccgaggcggt gtggccgtac gcgccgcacg tgccgcccct
     2761 gatcgacagg agggcgagat gagcacgatt accgacgtcc gggagcggat cgaggcccgg
     2821 ctggggatcg tctacgacat catggagccg gccgagcgtt cactcgtccc actccgcccg
     2881 cgggagcgat cgcacagtct ccccgccggc agcccgatct cgctggcggc ctacgtgatt
     2941 cgcaaggcgg agactgaccg gatcccgatc accgggagcg ccggcaccgt gatcggcacg
     3001 gtgaccctgg cggccgtcga ggacgccgtg gcctgcgcga gctggacgct gcaccacccg
     3061 gccgcccggc cggctcactg acgacgacgc cacccagaag gccgcgtcac tccggggcga
     3121 acttctcgaa gaacgtcggg agcgtcccga ggaaccccag cagcaccagg atggccgtga
     3181 gcgtgaacat gcctcctggc gacggatcct tcctgcgcag ggcgatgccg aggatgatcc
     3241 acgccaccac ccacgccgca acggcgtaga tggtcttccc cgagaggggg cccacccggt
     3301 cgttcagcgc cagcgcgtcg gcgaacgagg cgctcgcctc cgcgatcgtg gtcaggatcc
     3361 cgagcaccag cgacccgatc ccggcggcga ggagcgcggc ggcctccggg agctgcgccg
     3421 cggggagctg cgggcggatc cctccgcgga cccggctgca acgcagg
//
LOCUS       DASPWO010000537         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_157177, whole genome shotgun sequence.
ACCESSION   DASPWO010000537 DASPWO010000000
VERSION     DASPWO010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157177"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VEO00_12340"
     CDS             complement(<1..382)
                     /locus_tag="VEO00_12340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HYU58821.1"
                     /translation="MRDGPAQASRPPALPTLSVIMPSYNHARYLEDALRAHLDQSAPP
                     LEILVIDDASTDDSCARAERLAQQHPPLRLIRLSRNAGVNTASNLGLTEARGDYVCFS
                     AVDDLVLPGFAERSLELLARNPDAA"
     gene            complement(397..954)
                     /locus_tag="VEO00_12345"
     CDS             complement(397..954)
                     /locus_tag="VEO00_12345"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYU58822.1"
                     /translation="MTGLPDVQMRSPRIRRRRVVHRNPFWSVRAVRAEFDGFVKDYYV
                     ADFGARVGVVAVRAGAVLLTAQYRFLVDRVAWEIPGGRVDAGESAEEAARRECLEETG
                     VYCRGLRPLIAYRPGLDNVENLTTVFYSENVEERQLFVPDPAEVLAIAWVPLAECVGM
                     VFERRIADCLTASAVLGYRCRLEGR"
     gene            complement(951..1886)
                     /locus_tag="VEO00_12350"
     CDS             complement(951..1886)
                     /locus_tag="VEO00_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYU58823.1"
                     /translation="MRNSFASTFYEVGCRDHRLAVVVADISPAGSLEKFRQEFPGRFI
                     NTGVAEQVMIGMCAGMALRGLRPFAYTIATFALFRPFEFVRDDLCYQNLPVTVVGIGG
                     GVTYSTLGGTHHAQEDIAIATALPNMAVVAPCDPAETREATLWCARQERGPVYLRLGK
                     AGEPVLTREAAESWEFGKIRYVKRGADVCILGYGPIMRMAAEVGARLEGTGRSVSLVS
                     VHTLKPLDVDGIAEAIRRHRHVIVIEEMAPYGGLGPRVKEMAWDLGASCRLDIFSLED
                     AFHHVYGSHGELLAVHGLGVEAILARLKAEPGARR"
     gene            complement(1883..>2492)
                     /locus_tag="VEO00_12355"
     CDS             complement(1883..>2492)
                     /locus_tag="VEO00_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HYU58824.1"
                     /translation="CLAQYVILEDLGVLTKADLDLYCTAAGQLGAHPDYGVPGIEAST
                     GSLGHGMGIATGMAYADRIRSGDRRTHVVLSDGELQEGSTWEAIMMAANLGLDNLLAF
                     LDLNDFSGLERMSERHPALYPVVDKVRAFGWEVALVNGHDAVAAWEAVGTRRGGRPFM
                     LVGETVKGRGVSFMEHVPIWHYRSPSKEEYAKAIAELAEVAS"
BASE COUNT          396 a          910 c          837 g          349 t
ORIGIN      
        1 acgcggcgtc agggttgcgt gccagcagct ccagcgaccg ctcggcgaat ccgggcagca
       61 cgaggtcgtc caccgcggag aagcagacgt agtccccccg cgcctcggtc aggccgaggt
      121 tcgatgctgt attcacgccg gcgttgcggc tcagccggat caagcgaagg gggggatgct
      181 gctgcgccag ccgctccgcg cgggcgcagc tgtcgtccgt ggaggcgtcg tcgatcacga
      241 ggatctcgag cggcggcgcc gactggtcca ggtgggctcg cagcgcgtcc tccaggtagc
      301 gcgcgtggtt gtagctcggc atgatcaccg acagcgtcgg cagggccgga ggccgtgagg
      361 cctgggcggg cccatccctc atcggctgat ccggactcac cggccctcca gccgacaacg
      421 atacccgagc acggccgacg ccgtgaggca gtcggcgatg cggcgctcga acaccatgcc
      481 cacgcactcg gccagcggca cccaggcgat cgccagcacc tcggccgggt cgggaacgaa
      541 aagctgacgc tcttcgacgt tctcgctgta gaagaccgtc gtcaggttct ccacattgtc
      601 gagaccgggg cggtaggcga tgagcgggcg gaggccccgg cagtagacgc cggtctcctc
      661 caggcactcg cggcgcgcgg cttcctccgc cgattcccca gcgtccaccc gccctccagg
      721 aatctcccag gccacgcggt cgacgaggaa ccgatactgg gccgtgagca ggactgcgcc
      781 cgcgcgcacc gccacgacgc cgacccgggc accgaagtcg gccacgtagt agtccttcac
      841 gaagccgtcg aactccgccc gcacggcgcg gacgctccag aacgggttcc ggtgcaccac
      901 ccgccggcgg cggatccgcg gcgaccgcat ctggacgtcg ggcaggcccg tcaccggcgc
      961 gcccccggct ccgccttcag gcgcgccagg atggcttcca cgccgagccc atgcacggcg
     1021 agcaactccc cgtgcgatcc gtagacgtgg tggaaggcgt cctcgagcga gaagatgtcc
     1081 aggcgacagg aggcccccag gtcccaggcc atctccttga cccgtggccc gagcccgccg
     1141 tacggcgcca tctcctcgat gacgatcacg tgccggtgcc ggcggatcgc ctcggcgatc
     1201 ccatcgacat cgagcggctt cagcgtgtgg acggacacca gcgatacact ccgacccgtc
     1261 ccctcgagcc gggcccccac ttcggccgcc atcctcatga tcggcccgta gccgaggatg
     1321 cacacgtcgg cgcccctctt gacgtagcgg atcttcccga actcccacga ctccgccgcc
     1381 tcccgagtca gcacgggctc cccggccttc ccgagccgga gatacaccgg tccccgctcc
     1441 tgccgggcgc accacagcgt cgcctcgcgg gtctcggcgg gatcgcaggg cgcgacgacg
     1501 gccatgttgg gcagcgccgt cgcgatggcg atgtcctcct gggcgtggtg ggtgcccccc
     1561 agcgtagagt acgtcacgcc gccgccgatc cccacgaccg tcaccgggag gttctggtag
     1621 cagaggtcat cgcggacgaa ctcgaaagga cggaagagcg cgaaggtcgc gatggtgtag
     1681 gcgaagggac gcaggccgcg cagggccatg ccggcgcaca tgccgatcat gacctgctcg
     1741 gcgacgcccg tgttgatgaa ccggccgggg aactcctgac ggaacttctc gagcgagccc
     1801 gccggcgaga tgtcggcgac caccaccgcc agccgatggt cccggcagcc gacctcgtaa
     1861 aaggtgctgg cgaagctgtt cctcacgagg ccacctccgc cagctcggcg atcgccttcg
     1921 cgtactcttc cttgctggga gagcggtagt gccagatcgg cacgtgctcc atgaaggaca
     1981 ccccgcggcc cttgacggtc tcgcccacga gcataaaggg gcgtcccccg cggcgcgtgc
     2041 cgaccgcctc ccacgccgct acggcgtcgt gcccgttgac cagcgcgacc tcccacccga
     2101 aggcgcgcac cttgtcgacc acagggtaga gcgccgggtg tcgctcgctc atccgctcca
     2161 gccccgagaa gtcgttgagg tcgaggaagg ccaggaggtt gtcgaggccc aggttggcgg
     2221 ccatcatgat ggcctcccag gtcgagccct cctggagctc gccgtcggag aggaccacgt
     2281 gggtccgccg gtccccgctc cgaatccgat ccgcgtaggc cattccggtg gcgatgccca
     2341 tcccgtgccc gagcgacccg gtcgaggcct cgatgccggg gacgccgtag tcgggatgag
     2401 cgcccagctg gcccgcggcg gtgcaataga ggtccaggtc ggccttcgtg aggacaccca
     2461 ggtcctcgag gatgacgtac tgggccaggc ag
//
LOCUS       DASPWO010000538         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_76195, whole genome shotgun sequence.
ACCESSION   DASPWO010000538 DASPWO010000000
VERSION     DASPWO010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76195"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VEO00_12360"
     CDS             complement(<1..413)
                     /locus_tag="VEO00_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006380234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HYU58825.1"
                     /translation="MHGLAQESGPIQAVFRSFENNALWVILLVSLGALAFAYYLVREV
                     LAAPEGTPKMVEIARAIQEGARAYLNRQFRTVGLFLGLLTVIIFFILPAPKDALHSEL
                     SIKLGRSLAFILGAGFSAVTGYAGMWLAVRANVRTA"
     gene            complement(534..1820)
                     /locus_tag="VEO00_12365"
     CDS             complement(534..1820)
                     /locus_tag="VEO00_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HYU58826.1"
                     /translation="MTDAVAIAIFVGAYVLIATEWVHRVVVALAGAGLMLLFKIVDAN
                     DAFHSEEFGVDWNVVFLLFAMMIIVGVLRRTGFFEYVAIWSAKRSRGRPFAILTTLVL
                     ITAVASALLDNVTTILLIAPVTVLIAERLGVQPVPLLMAEVFASNIGGTATLIGDPPN
                     IIIGSAAGLSYLDFLANLGPIVAVLVAVFVFQSRWMFRRSLVSHPDRVEELMALDERE
                     ALLEGSLLPKALGVLGVVTLAFALQEPLGYEPSVVALLGAGVLLLISGGDRLDYLRDV
                     EWPTLVFFMGLFVMVGGLVKAGVIEALADRLIEFTDGRLFLATALLLVASAVLSGLVD
                     NIPYVATIVPLVGALAAAIPGDTTVLWWALALGAGLGGNATSIGASANVVVLGVAERA
                     GSRIRFTQFLRQGLPVTAISVAVALAYLWLRYFAFR"
     gene            complement(1817..2263)
                     /locus_tag="VEO00_12370"
     CDS             complement(1817..2263)
                     /locus_tag="VEO00_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYU58827.1"
                     /translation="MKARNLASPQETLRTDQEAGEAASVLSRYGVRAVLVVDPAGSFA
                     GVISDSELLRRLLPAYVTENEALARVLEEGAAEALQGRLEGLTVADLMPGDREEYPVV
                     DGEDTLVEVAATMVRSRASLVGVVDEGRLIGGISIDDLVSHLLRTR"
     gene            complement(2492..>2683)
                     /locus_tag="VEO00_12375"
     CDS             complement(2492..>2683)
                     /locus_tag="VEO00_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58828.1"
                     /translation="EVTDSGAPFDPDTYEGEPAAVDGEEFSFERGLSLPVVRGLVEDL
                     EIVARAEGGMRVRFTLRAG"
BASE COUNT          478 a          950 c          925 g          330 t
ORIGIN      
        1 gccgttcgca cgttggcgcg cacggcgagc cacatcccgg cgtagcccgt caccgcggag
       61 aaccccgcgc cgaggatgaa cgccagggaa cggccgagct tgatcgaaag ctcggagtgc
      121 agggcgtcct tcggtgccgg aaggatgaag aagatgatca cggtcagcag accgaggaag
      181 agcccgacgg tgcggaactg gcggttcagg taggctcgcg cgccctcctg gatcgcccgg
      241 gcgatctcga ccatcttcgg cgtcccctcg ggcgcggcca ggacctcgcg gaccaggtag
      301 taggcgaacg ccagcgcgcc gagcgacacc agcaggatca cccagagcgc attgttctcg
      361 aacgatcgga agacggcctg gatcgggccg ctttcctggg caaggccgtg catcgatcct
      421 cctcggtcgg attcgggaag gttttgctcc cggccgggcc ggggcccgag cagtatagag
      481 aagcgccaaa cgcgtttccc ccgcggccgg cgagcccggg ggggcgcggt cggtcagcgg
      541 aacgcgaagt accgcagcca caggtacgcc agggccacgg cgaccgatat ggccgtgacc
      601 ggcaacccct gccgcaggaa ctgggtgaat cggatccggg agccggcgcg ctccgccacg
      661 ccgagcacca ccacgttcgc gctggcgccg atcgacgtgg cgttcccgcc gaggcccgcg
      721 ccgagggcga gcgcccacca caacaccgtc gtgtccccgg ggatcgcggc agccagggcg
      781 ccgacgagcg gcacgatggt cgccacgtac gggatgttgt cgaccaggcc cgacagcacg
      841 gccgacgcca ccagcaacag ggccgtcgcc aggaagagcc ggccgtccgt gaactcgatc
      901 aggcgatcgg cgagcgcctc gatcacgccg gccttcacca acccgccgac catgacgaac
      961 agccccatga agaagacgag cgtcggccat tcgacgtcgc gcaggtagtc cagccggtca
     1021 ccgccggaga tcagcaacag cacgcccgcg cccaggagcg cgaccacgga cggctcgtag
     1081 ccgagcggct cctgcagcgc gaacgccagc gtgaccacgc cgaggacgcc gagcgccttc
     1141 gggagcagtg atccctccag cagggcctct cgctcgtcga gcgccatcag ctcctcgacc
     1201 cggtcggggt gggagaccag cgagcggcgg aacatccacc gagactggaa gacgaacacg
     1261 gcgacgagca ccgccacgat cggcccgagg ttcgccagaa agtccaggta ggagaggccg
     1321 gccgcgctcc cgatgatgat gttcggcggg tcaccgatca gcgtggccgt cccgccgatg
     1381 ttcgaggcga acacctccgc cataagcagt gggaccggct gcacacccaa gcgctcggcg
     1441 atcagcaccg tcaccggcgc gatcagcagg atcgtcgtca cgttgtcgag caaggcggac
     1501 gcgaccgccg tgatcaggac gagcgtggtg aggatggcga aggggcgccc gcgggatcgc
     1561 ttggccgacc agatggccac atactcgaag aacccggtcc gacgcaacac cccgacgatg
     1621 atcatcatcg cgaacagcag gaagaccacg ttccagtcga ccccgaactc ctcggagtgg
     1681 aacgcgtcgt tcgcatcgac gatcttgaac agcagcatca ggcccgcgcc ggcgagggcc
     1741 acgacgacgc ggtgcaccca ttcggtggcg atcaggacgt aggccccgac gaagatcgcg
     1801 atggcgaccg cgtcggtcat cgcgtccgga gcaggtggct caccaggtcg tcgatcgaga
     1861 tgccgccgat cagccgaccc tcgtcgacca ctccgacgag cgaggcgcgc gaccgcacca
     1921 tggtcgcggc gacctcgacg agggtgtcct cgccgtcgac caccgggtac tcctcgcgat
     1981 cgccgggcat caggtcggca accgtgagtc cctcgagcct cccctggagg gcctccgcgg
     2041 cgccctcctc cagcactcgc gccagcgcct cgttctccgt gacgtatgcg ggcaggaggc
     2101 gccggagcag ctcgctgtcg ctgatcacgc cggcgaacga tccggcgggg tcgaccacca
     2161 gcaccgcccg gacgccgtag cgggagagca cgctcgccgc ctcgccggcc tcctggtccg
     2221 tgcgaagggt ttcctgcggg ctcgccaggt tgcgcgcctt catggtgcca tccctcctcg
     2281 tcgccgtgag tgatccggcg gcagcgtacg cgcgagcgaa aacgggagga ggaggggagc
     2341 cacgtgcggg acgccggtgc accacgaagc ggcatcgggg taggggcgca tgccggggtc
     2401 tccctgccga ccagacttcc cggcacaccg acgaggcgtc ttcggttgaa acgatcctat
     2461 cggttccgcc ggcggcggcc gggcgagcgg ctcagccggc gcgcagggtg aagcggacgc
     2521 gcatgccgcc ctcggcacgg gcgacgatct cgaggtcctc gaccaggccg cgcacgacgg
     2581 gcagcgacag cccgcgctcg aacgagaact cctcgccgtc gacggcggcc ggctcgccct
     2641 cgtaggtgtc cgggtcgaac ggcgccccgg aatcggtcac ctc
//
LOCUS       DASPWO010000539         6215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_924892, whole genome shotgun sequence.
ACCESSION   DASPWO010000539 DASPWO010000000
VERSION     DASPWO010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924892"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /gene="rpsC"
                     /locus_tag="VEO00_12380"
     CDS             complement(<1..819)
                     /gene="rpsC"
                     /locus_tag="VEO00_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HYU58829.1"
                     /translation="MGHKVNPYGFRLGVIYPWKSNWYSRKEYAEQLHEDIRIRKYIRD
                     SMTRAGIASIDIDRRGDQITVWIWTARPGIVIGRKGSEVDRIRRELERLTGKRVDVKV
                     QDMNASTTELKPETNAQLLAQGVAEQLAGRVSFRRAMRRAVQTALRSGALGVRVQCGG
                     RLGGAEMSRREWYREGRVPLHTLRAKVDYGLAEAKTTFGRIGVKVWVYHGDEIPVEEQ
                     EIARARARALAGMPARGAGAASTGALITSTKDLAELAEAEPAAEPGAAPAEPEVP"
     gene            complement(809..1165)
                     /gene="rplV"
                     /locus_tag="VEO00_12385"
     CDS             complement(809..1165)
                     /gene="rplV"
                     /locus_tag="VEO00_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HYU58830.1"
                     /translation="MEARAVARYVRVSPQKARRVVDLIRGKHVEEARRVLRFAPIGAA
                     ETVSKVLNSAVANAEQAIADTRELATLYVSRATVDEGPTMKRWRPKDRGRAVRIRKRT
                     SHITVVVETIGETVGS"
     gene            complement(1179..1463)
                     /gene="rpsS"
                     /locus_tag="VEO00_12390"
     CDS             complement(1179..1463)
                     /gene="rpsS"
                     /locus_tag="VEO00_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HYU58831.1"
                     /translation="MPRSVKKGPFVDEHLLAKVEDANRRGDKRVIKTWSRRSTVVPEM
                     VGLTIAVHDGRKHVPVFVTEAMVGHKLGEFAPTRTFRTHGHHTERSTALK"
     gene            complement(1487..2317)
                     /gene="rplB"
                     /locus_tag="VEO00_12395"
     CDS             complement(1487..2317)
                     /gene="rplB"
                     /locus_tag="VEO00_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HYU58832.1"
                     /translation="MAIRKYKPTSAGRRGATVSDFGEVTRPKPEKALLAPKPKKAGRG
                     AQGRITTRHQGGGNKRRYRVIDFRREKDGVPARVAHVEYDPNRSARIALLHFADGEKR
                     YILAPTGVKVGDRLASGEGVDIRPGNALPLKNIPVGTVVHAVELRPGGGAKMARSAGT
                     SVQLVAKEGGYAQLRLPSGEMRLVYATCRATVGVVGNPEHELISWGKAGRNRWRGVRP
                     SVRGVAMNPVDHPLGGGEGKSSGGRHPTSPWGKKEGRTRKKGKPSDRYIVRRRGSKRG
                     "
     gene            complement(2328..2615)
                     /gene="rplW"
                     /locus_tag="VEO00_12400"
     CDS             complement(2328..2615)
                     /gene="rplW"
                     /locus_tag="VEO00_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002523451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HYU58833.1"
                     /translation="MKSPRDVIIRPIVSEKSYAGLEQNRYTFLVNPKANKTEIKEAIQ
                     AIWNVRVLKVNTNLRRGKVKRFRQTTGQRPSTKRAVVVLAEGDTIEIFEQK"
     gene            complement(2612..3304)
                     /gene="rplD"
                     /locus_tag="VEO00_12405"
     CDS             complement(2612..3304)
                     /gene="rplD"
                     /locus_tag="VEO00_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HYU58834.1"
                     /translation="MATVDVLDQSGKVVGTRDLDAEVFGAEVRPALMHQVVVAALAAR
                     RAGTHATKTRGEVRGGGRKPWRQKGTGRARAGSTRAPHWTGGGVVFGPHPRDHAQRLP
                     KKMRRAALRSALSAQAAEGRIRVVERLSFEEPKTREAAETLRALEAGGRVLLVLAGPD
                     QVVEKSFRNLPEVRVTYAGGLSTYEVLAADTLLFTADALALAGRNGPAAEGPAAPEGA
                     GSGGDDEEEAAS"
     gene            complement(3304..3978)
                     /gene="rplC"
                     /locus_tag="VEO00_12410"
     CDS             complement(3304..3978)
                     /gene="rplC"
                     /locus_tag="VEO00_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HYU58835.1"
                     /translation="MKGILGEKLGMTQIFTEDQRAVPVTVIKAGPCFVAQVKTTERDG
                     YQAVQLALGAVPERKARRPETGHAKAAGLDATPKYLVELRVDDVSGYKPGQEIRADVF
                     RPGERADVVGVSKGKGFSGVMKRHGFAGLSASHGTERKHRSPGAVGACATPSRIFKGT
                     RMAGQMGNVRVSVLNLEVIEVDPERGLLLLRGAVPGPTGGLVMVKSSSRAPAEEPAAA
                     AAGEAS"
     gene            complement(3992..4300)
                     /gene="rpsJ"
                     /locus_tag="VEO00_12415"
     CDS             complement(3992..4300)
                     /gene="rpsJ"
                     /locus_tag="VEO00_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HYU58836.1"
                     /translation="MAAPKIRIKLRGYDHVILDAAARDIADHVVRTGAKVVGPVPLPT
                     ERNLFTVIRSPFKDKDSREHFEMRTHKRLIDIHNATAKTVQELQRLNLSAGVDIEIKL
                     "
     gene            complement(4336..5523)
                     /gene="tuf"
                     /locus_tag="VEO00_12420"
     CDS             complement(4336..5523)
                     /gene="tuf"
                     /locus_tag="VEO00_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HYU58837.1"
                     /translation="MGKKKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLAEKGLADF
                     TPFEQIDKAPEERERGITIAIAHVEYETENRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHVLLARQVGVPYIVVALNKCDMVDDPELLDLVELEVRELLTS
                     YEFPGDDVPVVQVSALKALEGDPEWVAKIEELLAACDASIPEPTRDVDKPFLMPIEDI
                     FSITGRGTVATGRVDRGKLQKMSEVEIVGIKPTRKTVATDLEMFRKLLDEVQAGDNVG
                     VLLRGIDKDEIERGQVLAKPGSITPHTEFKAQVYVLSKDEGGRHTPFFNGYRPQFYFR
                     TTDVTGSIQIPGGPDSMVMPGDNTEMDVELIAPIAMEEGLRFAIREGGRTVGAGRVTQ
                     ISE"
     gene            complement(5526..>6215)
                     /gene="fusA"
                     /locus_tag="VEO00_12425"
     CDS             complement(5526..>6215)
                     /gene="fusA"
                     /locus_tag="VEO00_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018998535.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HYU58838.1"
                     /translation="LLREFRVEANVGKPQVAYRETIRKAAAAQGRYVRQTGGRGQYGH
                     CWLRLIPQDPGAGFTFDNATVGGTIPKEFINPIQKGIEEAMTGGILAGYPMVDLKVEV
                     FDGSYHDVDSSEMAFKIAGSMGFKDGATKAHPVLLEPIMEVEVVTPEEYMGDVIGDLN
                     SRRGKVHAMNPRSGVQMIEALVPLAEMFGYATDLRSRTQGRATYTMQFHSYQEVPAGI
                     SREIVAKVRGE"
BASE COUNT          781 a         2345 c         2025 g         1064 t
ORIGIN      
        1 gggcacctcg ggctcggccg gcgccgcccc cggctccgcc gccggctcgg cctcggccag
       61 ctcggcgagg tccttcgtcg acgtaatcaa ggcgccggtc gacgcggcgc cggccccgcg
      121 cgccggcatc ccggcgagcg cccgggcgcg tgcgcgggcg atctcctgct cctccaccgg
      181 gatctcgtcc ccgtggtaga cccacacctt cacgccgatc cggccgaagg tggtcttcgc
      241 ctccgccagc ccgtagtcca ccttcgcccg cagcgtgtgc agggggacgc ggccctcgcg
      301 gtaccactcg cggcgcgaca tctccgcgcc accgaggcgc ccgccgcact gcacccgtac
      361 gccgagcgcg cccgagcgca aggcggtctg caccgcccgg cgcatggccc gccggaacga
      421 gacccgcccg gcgagctgct cggccacgcc ctgggccagg agctgcgcgt tcgtctccgg
      481 cttcagctcg gtggtggagg cgttcatgtc ctgcaccttc acgtcgacgc gcttgccggt
      541 caggcgctcg agctcgcggc ggatgcggtc gacctcggag cccttgcgcc cgatgacgat
      601 gcctggccgg gcggtccaga tccacaccgt gatctggtcg ccgcggcgat cgatgtcgat
      661 gctcgcgatc ccggcccggg tcatgctgtc ccggatgtac ttccggatgc ggatgtcctc
      721 gtgcagctgc tcggcgtact ccttcctgga gtaccagttg ctcttccagg ggtagatgac
      781 ccccagccgg aacccgtacg ggttgacctt atgacccaac cgtctctccg atcgtctcca
      841 ccaccacggt gatgtggctc gtgcgcttgc ggatccgcac cgcgcggccg cggtccttcg
      901 gccgccaacg cttcatcgtc ggtccctcgt ccacggtcgc ccggctgacg tacagcgtcg
      961 ccagctcgcg ggtgtccgcg atcgcctgct cggcgttggc caccgccgag ttcagcacct
     1021 tcgacacggt ctcggccgcg ccgatcgggg cgaagcgcag cacccggcgg gcctcctcca
     1081 cgtgcttgcc gcggatgagg tccaccacac gccgcgcctt ctgcggcgag accctgacgt
     1141 agcgcgcgac cgctctcgct tccatcactc cccgtcccct acttgagcgc cgtgctccgc
     1201 tcggtgtggt gcccgtgcgt gcggaacgtc cgcgtcgggg cgaactcgcc gagcttgtga
     1261 ccgaccatcg cctcggtgac gaacaccggg acgtgcttgc ggccgtcgtg caccgcgatg
     1321 gtcaggccga ccatctccgg caccacggtc gaccgccgcg accacgtctt gatcacgcgc
     1381 ttgtcccccc gccggttcgc gtcctcgacc ttggccaaca ggtgctcgtc cacgaacggg
     1441 cccttcttca ccgacctcgg catctcgtct cccgactgtc ctctcgctac ccgcgcttcg
     1501 atccgcgccg gcgcacgatg tagcggtccg agggcttccc cttcttccgg gtccgcccct
     1561 ccttcttccc ccacgggctg gtcgggtggc ggccgccgga ggacttcccc tcaccaccac
     1621 cgagcggatg gtccaccggg ttcatcgcca cgccgcgcac cgacgggcgc acaccccgcc
     1681 agcggttgcg gccggccttc ccccaggaga tcagctcgtg ctccgggtta ccgacgacgc
     1741 caacggtcgc ccggcacgtc gcgtacacca ggcgcatctc cccggacggg aggcgaagct
     1801 gggcgtaccc accctccttc gccaccagct gcacggacgt tccggcggag cgcgccatct
     1861 tcgcgccgcc gcccgggcgc agctccaccg cgtgcaccac cgtgccgacg gggatgttct
     1921 tcagcggcag cgcgtttccc ggccggatgt cgacgccctc gccggaggcg agccggtcgc
     1981 cgaccttcac gcccgtcggc gccaggatgt agcgcttctc gccgtccgcg aagtgcagca
     2041 gggcgatccg cgcgctgcgg ttcgggtcgt actcgacgtg cgccaccctg gccggcacgc
     2101 cgtccttctc ccggcggaaa tcgatcaccc ggtagcggcg cttgttcccg ccgccctggt
     2161 gacgcgtggt gatccgcccc tgggcccccc gcccggcctt cttcggcttc ggggcgagca
     2221 gcgccttctc cggcttcggc cgcgtcacct cgccgaagtc ggacacggtc gcgccgcgcc
     2281 gcccggcgct tgtgggcttg tacttgcgga tcgccatcgt caggtcccta cttctgctcg
     2341 aagatctcga tggtgtcgcc ctcggccagc acgacgaccg cgcgcttggt cgaaggccgc
     2401 tgaccggtcg tctgccggaa ccgcttgacc ttccccctgc ggaggttcgt gttcaccttc
     2461 agcacgcgca cgttccagat cgcctggata gcttccttga tctccgtctt gttggccttc
     2521 gggttcacca ggaacgtgta gcggttctgc tccagcccgg cgtaggactt ctccgagacg
     2581 atcgggcgga tgatcacgtc gcgggggctc ttcacgacgc cgcctcctcc tcgtcatccc
     2641 cgccggaacc cgctccttcc ggcgcggccg ggccctccgc cgccggaccg ttccggccgg
     2701 ccagagccag cgcgtcggcg gtgaacagca gcgtgtccgc cgccagcacc tcgtaggtcg
     2761 acaggccgcc ggcataggtc acccgaacct cggggaggtt gcggaacgac ttctccacga
     2821 cctggtcggg accggcgagg accagcagca cgcgcccgcc ggcctcgagc gcccgcagcg
     2881 tctcggccgc ctcgcgcgtc tttggctcct cgaacgacag gcgctccacc acgcggatcc
     2941 gcccctcggc cgcctgcgcc gagagggccg agcgcagcgc agcgcggcgc atcttcttcg
     3001 gcagccgctg agcgtggtcg cgcgggtgcg ggccgaacac gaccccaccc ccggtccagt
     3061 gcggcgcccg ggtcgagccg gcccgggcgc gtccggtgcc cttctgccgc cagggctttc
     3121 gcccgccgcc gcgaacctcg ccgcgggtct tcgtggcgtg cgtgccggcg cggcgcgccg
     3181 cgagcgcggc caccacgacc tggtgcatca gcgcgggccg aacttccgcg ccgaacacct
     3241 cggcgtccag atcgcgcgtg ccgaccacct tgccggactg gtcgaggacg tccacggtgg
     3301 ccatcaggac gcctccccgg cggccgcggc ggcgggctcc tcggcgggcg cccgcgagga
     3361 cgacttgacc attaccagcc cgccggtcgg gccggggacg gccccgcgca gcaggagcag
     3421 gccccgctcc gggtccacct cgatcacctc gaggttcagc accgagacgc gcacgttgcc
     3481 catctggccg gccatccgcg tgcccttgaa gatgcgggaa ggcgtcgcgc aggcgccgac
     3541 cgccccgggc gagcggtgct tgcgctcggt gccgtggctg gccgacagtc cggcgaagcc
     3601 gtgccgcttc atgacgccgc tgaagccctt gcccttcgag acgccgacca cgtcggcgcg
     3661 ctcgccagga cggaacacgt cggcacggat ctcctggccc ggcttgtagc cggagacgtc
     3721 gtcgacgcgc agctccacca ggtacttggg cgtggcgtcg agcccggcag ccttcgcatg
     3781 cccggtctcg ggccggcgcg ccttgcgctc gggaacggcg cccagggcga gctggacggc
     3841 ctgatagccg tcgcgctcgg tcgtcttgac ctgggccacg aagcacggcc cggccttgat
     3901 gaccgtcacc ggcacggccc gctgatcctc cgtgaagatc tgggtcatgc cgagcttctc
     3961 gccgaggatg cctttcatct tccgcgaacc cctagagctt gatctcgatg tcgacgccgg
     4021 ccgacaggtt cagccgctgg agctcctgca cggtcttcgc cgtggcgttg tggatgtcga
     4081 tcagccgctt gtgcgtgcgc atctcgaagt gctcgcggct gtccttgtcc ttgaagggcg
     4141 accggatgac ggtgaacagg ttgcgctcgg tcggcagcgg taccgggccg acgaccttcg
     4201 cccccgtgcg cacgacgtgg tccgcgatgt cgcgggccgc cgcgtcgagg atgacgtggt
     4261 cgtacccccg gagcttgatc cggatcttcg gagccgccac ggtcctcgtt ccttcctctc
     4321 tccgtcggcc gccggctact cgctgatctg cgtgacccgg ccggcgccca cggtgcgtcc
     4381 gccctcgcgg atggcgaacc gcaggccctc ctccatggcg atcggcgcga tcagctcgac
     4441 gtccatctcg gtgttgtcac cgggcatgac catcgagtcg ggcccgcccg ggatctggat
     4501 cgagccggtc acgtcggtcg tccggaagta gaactgcggc cggtagccgt tgaagaacgg
     4561 cgtgtgccgg ccaccctcgt ccttggacag cacgtagacc tgcgccttga actccgtgtg
     4621 cggggtgatc gagccgggct tggcgagcac ctggccgcgc tcgatctcgt ccttgtcgat
     4681 cccgcggagc aggacgccca cgttgtcgcc ggcctgaacc tcgtcgagga gcttgcggaa
     4741 catctcgagg tcggtggcaa cggtcttgcg ggttggcttg atcccgacga tctcgacctc
     4801 ggacatcttc tgcagctttc cgcgatccac tcgtccggtc gcgaccgtcc cgcgcccggt
     4861 gatcgagaag atgtcctcga tcggcatcag gaacggcttg tccacgtcgc gcgtcggctc
     4921 ggggatgctg gcgtcgcagg ccgccagcag ctcctcgatc ttggccaccc actcggggtc
     4981 gccctcgagc gccttcagcg ccgagacctg gaccaccggg acgtcgtcgc cggggaactc
     5041 gtaggaggtg agcagctcac gcacctcgag ctccaccagg tcgagcagct ccgggtcatc
     5101 caccatgtcg cacttgttca gggcgaccac gatgtacggc accccgacct gccgggcgag
     5161 cagcacgtgc tcacgggtct ggggcatcgg accatcggcc gccgacacca ccaggatcgc
     5221 gccgtccatc tgggcggcgc cggtgatcat gttcttgatg taatcggcgt gtcccggaca
     5281 gtccacgtgc gcgtagtgac ggttctccgt ctcgtactcg acgtgagcga tcgcgatcgt
     5341 gatgcctcgc tcccgctcct ccggcgcctt gtcgatctgc tcgaacggcg tgaagtcggc
     5401 caggcccttc tcggccagca ccttcgtgat cgcggccgtg agcgtggtct tgccgtggtc
     5461 gatgtggccg atcgtgccga tgttcacgtg cggcttggtc cgctcgaact tcttcttgcc
     5521 catcgctact cccctctgac cttggccacg atctcccggg agatgccggc cgggacttcc
     5581 tggtacgagt ggaactgcat cgtgtaggtg gctcgcccct gggtgcgcga ccgcaggtcg
     5641 gtggcgtagc cgaacatctc cgcgagcggc acgagcgcct cgatcatctg cacgcccgag
     5701 cgcgggttca tcgcatggac cttgccgcgg cggctgttca ggtcgccgat gacgtcgccc
     5761 atgtattcct cgggcgtcac cacctcgacc tccatgatcg gctcgagcag gaccgggtgc
     5821 gccttcgtgg cgccgtcctt gaagcccatc gacccggcga tcttgaacgc catctcggac
     5881 gagtcgacgt cgtggtagct gccgtcgaag acctcgacct tcagatcgac catcgggtag
     5941 cccgcgagga tgccgcccgt catggcctcc tcgatgccct tctggatcgg gttgatgaac
     6001 tccttcggga tcgtcccgcc gaccgtggcg ttgtcgaagg tgaaccctgc gcccggatcc
     6061 tgcgggatga gccgcagcca gcagtggccg tactggccgc ggccgccggt ctgccgcacg
     6121 tagcggccct gcgccgcggc ggccttccgg atcgtctcgc gatacgcgac ctggggcttg
     6181 ccgacgttgg cctcgaccct gaactcgcgc aggag
//
LOCUS       DASPWO010000540         9528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1176613, whole genome shotgun sequence.
ACCESSION   DASPWO010000540 DASPWO010000000
VERSION     DASPWO010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9528
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176613"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VEO00_12430"
     CDS             <1..887
                     /locus_tag="VEO00_12430"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HYU58839.1"
                     /translation="RTLEAMQLDTKRTLDALVARHAGSEAQRDRILANRFYARIADTL
                     AGTHEYMAMEKLYELATEGDHETIVVDTPPTRSALSFLDAPRRMTDFLGGRFLRFMLA
                     PTSVGARGTLRLTALGAQAFGRALRAITGGELLADTAEFLSAFEGMYDGFKRRAAAVL
                     DLLRRPGTRFVIVTSPEPGAMDEAGYFAERLAEAGMPLAAVVANRTHLGRPRLDPAVA
                     GAVPRLEGGAPAARALAAAIRTRLAWQAVEDREAAALDAFHARHGGVLLTTVPDLGRD
                     VHDLPGLRRLASALFEGG"
     gene            1013..3445
                     /locus_tag="VEO00_12435"
     CDS             1013..3445
                     /locus_tag="VEO00_12435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HYU58840.1"
                     /translation="MATKTTDRSPETPSAPVVMGREAPVHLPVTLLRLLLILFVTGVA
                     AGAFALMLAPTTMAAGKAVKQFTDQFTAPSPEDLSFPRFPERSTIYAADGKTRLATLY
                     LDENRKIVRLQNVAPVAQQAVLASEDKDFYEHGPLDLAAIIRAIIANWRAGEIEQGAS
                     TITQQLIKGVTNQDDPTLKRKLDEARLAFQLEQQYTKDEILELYLNEIYYGRGSYGIQ
                     AAAEFYFATSAKRLDLAQSATLAQLIPAPEAFNPVTAPDVAMRRRNRVIDEMLGLGWT
                     NQADASAAKERPLGLSKKLRAQSNRPGEAPYFVEYVRQEILRDPAFGENRSAREHTLY
                     QGGLKIITTLDPNWLQYAKDSVTSNMYDPNFPQAAVVTVDANSGAIRTLLGGTDFKKQ
                     KFDIASQGRRQAGSSFKPFTLAAALEQGIPAGRVYDSSPKTFTECGSPWTPHNAEGGS
                     EGYLNLYQATARSVNLVFARLIVDTGPQNVVSAAHRMGITSDLPAVCSLTLGSGPVSP
                     LEMASAYATLANDGVHCQPYAVQRAIGPNGKLIKRHRPKCTQAIESRWARQISDMLRG
                     VVTGGTGTAAALYHHPVVGKTGTTNDHADGWFVGYTWDQVATAVWVGDIRAENAIPDL
                     PGPWGPLFGGNLPATIWHDYMDRATASLPVNYFPPPPPIPRASVPNVIGKPFDEAVAI
                     LAEANFTATVQEVDSDAPAGTVVAQSPGPGASVTAGSLVTLSVSTGEAPNTPVPNVVG
                     RTLPDARQILQNAGFAVKIVHQTVKRPFLDGIVIGQDPNGGKHKQGSTVVLTVGKYQP
                     EPSPPPSPPPTP"
     gene            complement(3500..3967)
                     /locus_tag="VEO00_12440"
     CDS             complement(3500..3967)
                     /locus_tag="VEO00_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HYU58841.1"
                     /translation="MTADTTPDDPKARLSADIVAALKAGEKVRLAALRFLAYQVKNRE
                     VELRRELTAEEVGEVATRDTKRRRESIEAFEAGGRQDLVDKERAELAALESYLPPSLS
                     VEELDRLVEEAVAETGAATPAQMGQVMKALMPKVKGRADGAVVSARVRAQLGG"
     gene            3984..4859
                     /locus_tag="VEO00_12445"
     CDS             3984..4859
                     /locus_tag="VEO00_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYU58842.1"
                     /translation="MLWLWILLAVLGAGALCVAYGVLVERVWFRLNRHRLDILPPGSP
                     PLSVLHLSDLHMLGRQRRKARFLRSLPAADVTVVTGDLLGEPAAVDPVVAALHGVRGA
                     LASYFVLGSNDYFAPRPLNYLRYFGGLSGNGKRERVPGRAGDLAAALQADGWTHLRNR
                     RTELAANGSRIEVLGLDNPHIHRQDSRAIPRRHPSAFGLAVVHSPDPAPELAAFGYDL
                     VLSGHTHGGQVRLPLVGALVTNCSLPRRMARGLTRLGRAVLHVSSGAGTSKYAPFRFL
                     CRPEACLLELRPAPI"
     gene            complement(5086..6114)
                     /locus_tag="VEO00_12450"
     CDS             complement(5086..6114)
                     /locus_tag="VEO00_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYU58843.1"
                     /translation="MRIAQLAPPWLAVPPPAYGGIEWVVALLADGLTEAGHDVTLIAT
                     GDSRTKAKLEYVFEEGPGPKLINSIYHDTLHTLYAYRDVSRFDLIHLHTPFSALAAAP
                     HLAVPVVHTLHGSFADIMVRLYALVADRVWYVAISQNQRSHDPDLNYGGVVYNGIDTS
                     LYPFREEKEDFVLFVGRADPDKGALRAVRAARLAGVRLVMTMKIADPREEEHWARDIE
                     PELPEGTTVLGEITVQEKADLMSRARAVLFPIDWEEPFGLVMTESMACGTPVIARPRG
                     SVPEVIADGETGFIVDTDEEIAEAIGKAHSIDPKACRRRVEERFSKDAMVRGYERIFE
                     RVLASRGP"
     gene            6233..6727
                     /locus_tag="VEO00_12455"
     CDS             6233..6727
                     /locus_tag="VEO00_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58844.1"
                     /translation="MADQAGASSEAPTYTHQGTRYLLGYVADYYGIWDRQSPGVAVYR
                     FIKDPGGWQQAWTQYVALEPQGAAPTQPVAASARAGAAPVAAPAPATTLRLPSPARRT
                     LVALLRGLGWAALIVFPITGIAILAAGGGFSGVMVGFSDFVLGGLLWALGLGVAKVLE
                     AEAG"
     gene            6778..6851
                     /locus_tag="VEO00_12460"
     tRNA            6778..6851
                     /locus_tag="VEO00_12460"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6812..6814,aa:Pro,seq:cgg)
     gene            6865..8286
                     /locus_tag="VEO00_12465"
     CDS             6865..8286
                     /locus_tag="VEO00_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58845.1"
                     /translation="MTGPIPPAGRGPRGHALAVWALFASLLGLALFFLIRPAVGGDPY
                     YRPRPSGTTYAYWHLVALAFVPYGLALWSRRRRGRRGGPSAGVLIAGAAIVYAAFVPA
                     PAQQSQDVYQYLVYGRMAIHGANPYVVAPNEATGPWTPWLQWSLYDDTPTVYGPAWTL
                     LTAGVVRGATEDLLGSFLTLKAITAMMALGAAGLLARAVSRGGPGLRRDPGFVVLAFA
                     WNPLVVVSVGLGAHADAAVALLLAAAVVAERNRQDPVVTLLLAVATAVKPYAGVALLA
                     WLFAVARRGSARSLVPNTLLAGLVAAALYAPYWAGVRTLTAFAEGAWSVSASLTGTMI
                     RLLSGHVDDAVAAGATGAAVALRVVAGLVMVAAIVAAARWPRIRAEPWRAAALLFAAY
                     VLLTPWYLYWHLIGLIALASLVTDDRLTRGVGLFGATSVLTFTGGPAIGLLAQTIVRY
                     GPPILAAGLPGWVRDRALGERGR"
     gene            8301..8477
                     /locus_tag="VEO00_12470"
     CDS             8301..8477
                     /locus_tag="VEO00_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58846.1"
                     /translation="MGPITVRAARPGDFAAVATLLAELGRPAVLGGPDEDRYRGRYVA
                     WLADPDRYVRRRRR"
     gene            8482..8904
                     /locus_tag="VEO00_12475"
     CDS             8482..8904
                     /locus_tag="VEO00_12475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU58847.1"
                     /translation="MIGLVDMAIVPRLNFLAPQAWIPDLVVTRGARGRGAGAALLVRA
                     EEAARERGAWAVRLESANWRARAHAFYLREGYSDDAKSFDKALAELGWAPPTGPEELN
                     APGHPGAVIAARSRSPFAAIRPRSPARGIHGRWSSSKR"
     gene            complement(8796..9236)
                     /locus_tag="VEO00_12480"
     CDS             complement(8796..9236)
                     /locus_tag="VEO00_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HYU58848.1"
                     /translation="MNGPDREPGCIFCSIIAGESPATVVFEDERTLSFMDVNPATEGH
                     ALVVPRRHGLDLFEVPAEDAAAMWGTARLVAAAAKEAYRAPAVNLLMANGREAFQTVF
                     HAHVHVVPRYRFDELHLPWIPRAGDRGRIAANGERLRAAMTAPG"
     gene            complement(9233..>9528)
                     /locus_tag="VEO00_12485"
     CDS             complement(9233..>9528)
                     /locus_tag="VEO00_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HYU58849.1"
                     /translation="ELEPGLDAARAAIEAFPGVALVDDPDGDAFPTPLDAAGADDVLV
                     GRLRRAPDDRNALLLFSAADNLRKGAALNAVQIAERLVADGLLGGGFDVPAAR"
BASE COUNT         1259 a         3570 c         3330 g         1369 t
ORIGIN      
        1 gccggaccct cgaggcgatg cagctcgaca cgaagcgaac gctcgacgcc ctcgtcgcgc
       61 gccatgccgg atccgaggcc cagcgcgacc ggatcctggc gaaccgcttc tacgcccgga
      121 tcgccgacac ccttgccggg acgcacgagt acatggcgat ggagaagctc tacgaactgg
      181 cgaccgaagg agaccacgag acgatcgtcg tcgacacgcc tcccacccgc tccgcgctct
      241 cgttcctcga cgctccgagg cggatgaccg acttcctggg cggccgcttc ctgcggttca
      301 tgctcgcccc gacctcggtc ggggcccgcg ggacgcttcg gctgaccgcg ctcggcgcgc
      361 aggccttcgg ccgggccttg cgcgcgatca ccggcgggga gctgctggcc gacaccgccg
      421 agtttctgtc ggcgttcgaa gggatgtacg acggcttcaa gcgccgcgcg gccgctgttc
      481 tcgacctgct ccgccggccc gggacccgct tcgtgatcgt cacgtcgccg gagcccggcg
      541 cgatggacga ggccgggtac ttcgcggagc gcctcgcgga ggcgggcatg ccgctggcgg
      601 ccgtcgtcgc gaaccgcacc cacctcggcc ggccccggct ggatcccgct gtcgcgggtg
      661 ccgtccctcg gctggagggc ggcgccccgg ccgcccgtgc gctcgccgcc gccatccgca
      721 cgcgcctggc gtggcaggcg gtcgaggacc gggaagccgc cgccctcgac gcgttccacg
      781 ctcgccacgg cggggtcctg ctgacgacgg ttcccgatct cggccgggac gtccacgacc
      841 tgcctggcct gcgccgcctg gcgtcggcat tgttcgaggg gggctaggcg gcgtcggcgg
      901 cgagcgcgcg ggcggcgcgg ttggcgaggg ccatgatcgt ccaccggccc actgaacggc
      961 ggtcagtggg ccgtggccgt cggcagggcc gcacggtatc ctcacccggc ctgtggctac
     1021 caagacgacg gatcgctcgc ccgagacccc gagcgctccc gtcgtgatgg gccgggaagc
     1081 gccggtgcac ctccccgtga cgttgctgcg cctgctgctg atcctgttcg tcaccggggt
     1141 cgccgccggg gcgttcgcgc tcatgctcgc gccgaccacg atggctgccg ggaaggccgt
     1201 gaagcagttc acggaccagt tcacggcgcc ctcgccggag gacctgtcgt tcccccggtt
     1261 cccggagcgc tcgacgatct acgccgccga cgggaagacc cggctggcga cgctctacct
     1321 cgacgagaac cgcaagatcg tccggctgca gaacgtcgcg ccggtcgcgc agcaggccgt
     1381 cctggcgtcc gaggacaagg acttctacga gcacggacct ctcgacctgg cggcgatcat
     1441 ccgcgcgatc atcgcgaact ggcgggcggg cgagatcgag cagggcgcgt cgaccatcac
     1501 ccagcagctg atcaagggcg tcacgaacca ggacgatccg acgctgaagc gcaagctcga
     1561 cgaggcgcgc ctcgcgttcc agctcgagca gcagtacacg aaggacgaga tcctcgagct
     1621 gtacctgaac gagatctact acgggcgggg gtcctacggc atccaggccg cggccgagtt
     1681 ctacttcgcc acctcggcca agcgcctcga cctcgcccag tcggcgaccc tggcgcagct
     1741 gatccccgcg ccggaggcct tcaacccggt caccgctccg gacgtggcga tgcgccgccg
     1801 caaccgcgtg atcgacgaga tgctcgggct cggctggacc aaccaggccg atgcgtcggc
     1861 ggcgaaggag cgcccgctgg gtctctcgaa gaagctgcgc gcccagagca acaggccggg
     1921 ggaagcgccg tacttcgtcg agtacgtgcg gcaggagatc ctgcgcgacc cggccttcgg
     1981 cgagaaccgc agcgcgcggg agcacacgct ctaccagggc gggctgaaga tcatcaccac
     2041 gctcgacccg aactggctgc agtacgcgaa ggattccgtc acctcgaaca tgtacgaccc
     2101 gaacttcccg caggcggccg tggtcacggt cgatgcgaac agcggcgcca tccggaccct
     2161 cctcggcggt acggacttca agaaacagaa gttcgacatc gccagccagg gtcgccgcca
     2221 ggccggctcg tcgttcaagc cgttcacgct ggcggcggcc ctcgagcagg gcatcccggc
     2281 ggggagggtc tacgactcga gcccgaagac cttcaccgag tgcggctccc cctggacccc
     2341 acacaacgcc gagggcggga gcgagggcta cctgaacctg taccaggcca ccgcgcgctc
     2401 ggtgaacctg gtcttcgccc gcctgatcgt cgacaccggc ccgcagaacg tggtcagcgc
     2461 ggcgcaccgc atggggatca cgtcggacct gccggcggtg tgttccctca cgctcgggtc
     2521 cggtccggtg tccccgctcg agatggcctc ggcctacgca accctcgcga acgacggcgt
     2581 gcactgccaa ccgtacgcgg tgcagcgggc gatcggaccg aacggtaagc tgatcaagcg
     2641 gcacaggccc aagtgcacgc aggccatcga gtcgagatgg gcccgccaga tctcggacat
     2701 gctccgcggg gtcgtcaccg gcggcaccgg gacggccgcg gcgctgtacc accaccccgt
     2761 cgtcggcaag accgggacca cgaacgacca cgccgacggc tggttcgtcg gctacacctg
     2821 ggaccaggtg gccacggcgg tgtgggtcgg cgacatcagg gccgagaacg cgatcccgga
     2881 ccttccgggc ccgtgggggc cgctgttcgg cggcaacctg ccggccacga tctggcacga
     2941 ctacatggac cgcgccaccg cgagcctgcc ggtcaactac ttcccgccgc cgccaccgat
     3001 tccgcgggcg agcgttccga acgtgatcgg caagccgttc gacgaggcgg tagcgatcct
     3061 ggccgaggcg aacttcaccg ccaccgtcca ggaggtcgac tcggacgcgc cggccggcac
     3121 cgtggtcgcg cagtcgcccg gccccggcgc ctcggtgacg gcgggcagcc tggtgacgct
     3181 ctccgtctcc acgggggagg ccccgaacac cccagtgccg aacgtcgtcg gcaggacgct
     3241 ccccgacgcc cggcagatcc tgcagaacgc cggcttcgcc gtgaagatcg tccaccagac
     3301 cgtgaagcgg ccgttcctcg acggcatcgt catcggccag gacccgaacg gcggaaagca
     3361 caagcagggc tctacggtcg tcctcacggt cggcaagtac cagccggagc cctcgccgcc
     3421 accctcgccg ccgccaacgc cctgaccccg cgggcggagg gtgcgcccca atagtacagc
     3481 aactgtacta gtgagagggt tagcctccca gttgcgcgcg cacccgggcg ctgaccacgg
     3541 cgccgtcggc gcggcccttg accttcggca tcagggcctt catcacctgg cccatctggg
     3601 cgggggtggc ggcaccggtc tcggcgaccg cctcctcgac cagacggtcg agctcctcga
     3661 ccgacaggct tggcggcagg taggactcca gcgcggcgag ctccgcgcgc tccttgtcga
     3721 ccaggtcctg ccggccgccg gcctcgaacg cctcgatcga ttcgcgccgc cgcttcgtgt
     3781 cgcgcgtggc gacctccccg acttcctcgg cggtcagctc gcgacggagc tccacctcgc
     3841 ggttcttgac ctggtatgcg aggaagcgga gcgccgccag ccggaccttc tcgccggcct
     3901 tcagcgcggc gacgatgtcc gccgacagac gggccttggg gtcgtcgggt gtggtatccg
     3961 cggtcactag actcccctct ccgatgctct ggctctggat ccttctcgcc gtgctcggcg
     4021 ccggcgccct gtgcgtcgcc tacggcgtgc tggtcgaacg ggtctggttc cggctgaacc
     4081 ggcaccgact ggacatccta ccgccgggct caccgccgct ctccgtgctg catctctcgg
     4141 acctgcacat gctcgggcgg cagcggcgca aagcgcggtt cctgcgctcc ctccccgccg
     4201 ccgacgtgac cgtcgtcacc ggcgatctac tcggagagcc ggcggccgtc gaccccgtgg
     4261 tggcggcgct gcacggcgtg cggggagcac tcgcctccta cttcgtgctc ggctcgaacg
     4321 actacttcgc gccgcgcccg ctgaactacc tgcgctactt cggcggcctt tccggcaacg
     4381 ggaagcgcga gcgcgtgccg ggccgcgccg gcgacctcgc cgccgccctg caggccgacg
     4441 gctggaccca cctgcggaac cggcggacgg agctcgcggc gaacggttcc cggatcgagg
     4501 tgctcggcct ggacaacccg cacatccacc gccaggacag ccgcgcgatc ccacggcgcc
     4561 acccctcggc cttcggcctg gccgtcgtcc actcccccga ccccgcgccg gagctggccg
     4621 ccttcggtta cgacctggtc ctctccggcc atacgcacgg tggccaggtc cgcctcccgc
     4681 tggtcggcgc gctcgtcacg aactgctcgc tcccccggcg catggcccgc ggcctgaccc
     4741 ggctcggccg cgcggtcctg cacgtctcct ccggcgccgg caccagcaag tacgccccct
     4801 tccgcttcct ctgccgcccc gaagcctgcc tcctggagct gcgcccagcc cctatctagg
     4861 cgcgccaccg ccgtgggaga tccacacgcc cgggggtggc gggggcgcgc tcctacggtc
     4921 gggcgggcga agtcgctccg ggctgcgctc gccgaccgga agacgactcc aagacccgct
     4981 gggcacccgc gcggcggttt ccctgcgcga ggagcgggcg ggcccgaacg accgccgtca
     5041 cgctggtgca agggagtctc gggcccgccc gcgacccgag cgtcgtcagg gacctcttga
     5101 ggcgaggacg cgctcgaaga tccgttcata gccgcggacc atggcgtcct tggagaacct
     5161 ctcctcgacg cggcggcggc aggccttggg gtcgatggag tgggccttgc cgatggcctc
     5221 ggcgatctcc tcgtcggtgt cgacgatgaa gcccgtctcg ccgtcggcga tcacctcggg
     5281 gaccgagccg cgcgggcggg cgatcaccgg ggtgccgcag gccatcgact cggtcatcac
     5341 cagcccgaac ggctcctccc aatcgatcgg gaacagcacg gcccgggccc gcgacatcag
     5401 gtcggccttc tcctggaccg tgatctcgcc gagcaccgtg gtcccctcgg gcagctcggg
     5461 ctcgatgtcg cgggcccagt gctcctcctc gcgggggtcc gcgatcttca tcgtcatcac
     5521 cagccggacg ccggccaggc gtgccgcgcg cacggcgcgc agcgccccct tgtccgggtc
     5581 ggcccggccg acgaacagca cgaagtcttc cttctcctcg cggaaggggt agagcgacgt
     5641 atcgatcccg ttgtagacca cgccgccgta gttcaggtct gggtcgtggg agcgctggtt
     5701 ctggctgatc gccacgtacc acacccggtc ggcgaccagt gcatacagcc ggaccatgat
     5761 gtcggcgaac gacccgtgca gcgtgtgcac caccgggacg gcgaggtgcg gggcggccgc
     5821 cagggcggaa aacggagtgt gcaggtggat caggtcgaag cgcgagacgt cgcggtacgc
     5881 gtacagggtg tgcagcgtgt cgtggtagat cgagttgatc agcttcgggc cgggcccctc
     5941 ctcgaagacg tactcgagct tcgccttcgt ccgtgagtcc cccgtggcga tcagtgtcac
     6001 gtcgtggcct gcctcggtca ggccgtcggc gaggagcgcc accacccatt cgatcccgcc
     6061 gtaggcgggc gggggcaccg cgagccacgg cggcgcgagc tgggcgatac gcatcctgca
     6121 gtcttacccg aaacagggcc ggggtacgaa ccggctttac tcggccccgt ccggaccgcc
     6181 atcatccccg gtgacggccc gctccgagga accgggacga aaggagcaga cgatggcgga
     6241 ccaagccggg gcctcctccg aggcgcccac ctacacccac cagggaaccc gctacctctt
     6301 ggggtacgtc gccgactact acggcatctg ggaccgtcag tcgccggggg tggccgtcta
     6361 ccgcttcatc aaggatccgg gaggctggca gcaggcctgg acgcagtacg tggcgctcga
     6421 gccgcagggt gcggccccga cacagcccgt ggccgcctcg gcacgcgcgg gcgcggcgcc
     6481 ggtcgccgcg ccggccccgg caacgacgct caggctgccg tcacccgccc gccggacgct
     6541 cgtcgccctg ctccgcgggc tggggtgggc cgcgctgatc gtgttcccga tcaccggcat
     6601 cgcgatcctc gccgccggcg gcggcttctc cggcgtgatg gtcgggttct cggacttcgt
     6661 gctcggcggg ctgctgtggg cgctcggcct cggcgtggcg aaggtgctcg aggccgaggc
     6721 gggctgaccg gccgcaccgt gccgcgccga acggaccact acaatcgatc tctctcacgg
     6781 gacgtggcgc agtttggtag cgcgcagcgt tcgggacgct gaggtcgcgg gttcaaatcc
     6841 cgccgtcccg acctccgggg tgcagtgacc ggccccatcc caccggcggg tcgcggtcct
     6901 cgcggccacg cgctcgctgt gtgggcgctg ttcgcctcgc tgctcggcct ggccctgttc
     6961 ttcctgatcc gccccgcggt cggcggcgat ccctactatc ggccccgtcc gtcggggacg
     7021 acatacgcct actggcacct cgtcgcgctc gccttcgtcc cgtacgggct cgccttgtgg
     7081 tcccggcggc ggcgcggccg gcgcgggggc ccgtcggccg gcgtcctgat cgccggcgcc
     7141 gcgatcgtct acgcggcctt cgtcccggcg cccgcgcagc agtcccagga tgtctaccag
     7201 tacctggtgt acggacggat ggccatccac ggcgcgaacc cctacgtcgt ggccccgaac
     7261 gaggcgaccg ggccgtggac accgtggctc cagtggtccc tgtacgacga cacccctacc
     7321 gtctacggcc ccgcctggac gcttctcacg gccggcgtgg tccgcggcgc gacggaggac
     7381 ctcctcggct cgttcctgac gctgaaggcg atcaccgcga tgatggcgct cggggccgcc
     7441 gggctcctcg cccgggcggt ctcgcgcggc gggcccggcc tgcgccgcga cccgggcttc
     7501 gtggtgctgg cgttcgcctg gaaccctttg gtggtcgtgt cggtgggtct cggcgcgcac
     7561 gccgacgccg ccgtggcgct ccttctggcg gcggccgtcg tcgccgagcg gaaccgccag
     7621 gatccggtgg tcacgctgct cctcgccgtg gcgacggccg tgaagccgta cgccggggtc
     7681 gcactgctcg cctggctgtt cgcggtggcc cgccgcggaa gcgcccgctc cctcgtcccg
     7741 aacacgttgc tggccggcct ggtggcggcc gcgctgtacg cgccgtactg ggcgggggtc
     7801 cgcacgctga cggccttcgc cgagggcgcg tggagcgtct cggcctccct caccgggacc
     7861 atgatccggc tgctgtccgg acacgtcgac gacgccgtcg ccgccggcgc gaccggcgca
     7921 gcggtcgccc tccgggtcgt cgcggggctg gtgatggtcg cggcgatcgt ggcggcggcc
     7981 cggtggccgc ggatccgggc ggagccgtgg cgggccgcgg ccctcctgtt cgccgcctac
     8041 gtgctgctca cgccctggta cctctactgg cacctgatcg gcctgatcgc cctcgcgtcg
     8101 ctcgtcaccg acgaccggct cactcgcgga gtgggcctct tcggcgcgac ctccgtgctg
     8161 accttcacgg gggggcccgc gatcgggctc ctcgcccaga cgatcgtccg ctacggcccg
     8221 cccatcctgg ctgccgggct ccccggatgg gtccgcgacc gcgccctcgg cgagcggggc
     8281 cggtagcatc ggccgtccag atgggaccga tcacggtgcg cgcggctcgt cccggcgact
     8341 tcgcggccgt cgcaacgctc ctcgcggaac tggggcgtcc ggcggtcctg ggcggccccg
     8401 acgaggaccg ctaccgcggc cgctacgttg cctggctggc cgaccccgat cggtacgttc
     8461 gtcgccgaag acggtgacgt ggtgatcggc ctggtggaca tggcgatcgt cccccggctg
     8521 aacttcctgg cgccccaggc ctggatcccc gacctggtgg tgaccagggg agcgcgaggc
     8581 cgcggcgccg gcgcggccct cctggtccgg gccgaggagg ccgcccgcga gcgcggcgcg
     8641 tgggccgtgc gcctcgagtc ggcgaactgg cgcgcccgag cgcatgcctt ctatctccgg
     8701 gagggttatt cggacgacgc gaagagcttc gacaaggccc tcgccgagct cggctgggcc
     8761 ccgccgaccg ggcccgagga gctgaacgct cccggtcacc cgggcgcggt catcgcggcc
     8821 cgcagccgct cgccgttcgc ggcgatccgg ccgcggtcgc cggcccgcgg gatccatggc
     8881 aggtggagct cgtcgaagcg gtagcgcggg acaacgtgca cgtgggcgtg gaacacggtc
     8941 tggaacgcct cgcgcccgtt cgccatcagc aggttgacgg ccggcgcccg gtaggcctcc
     9001 ttcgccgccg ccgcgacgag ccgcgcggtc ccccacatcg ccgcggcgtc ctcggccggg
     9061 acctcgaaca gatcgaggcc atggcgcctg gggacgacca gcgcgtgccc ctcggtggcc
     9121 gggttgacgt ccatgaagga gagcgtccgc tcgtcctcga agacgacggt cgcggggctc
     9181 tctccggcga tgatggagca gaagatgcag ccgggctcgc ggtcgggacc gttcaccgcg
     9241 cggccgggac gtcgaacccc ccgccgagca ggccgtcggc caccaggcgc tcggcgatct
     9301 ggaccgcgtt cagggcggcg cccttgcgca ggttgtcggc cgcggagaac agcaggagcg
     9361 cgttccggtc gtcgggcgcc cggcgcagcc gcccgaccag gacgtcgtcg gcgccggcgg
     9421 cgtcgagcgg cgtcgggaag gcatcgccgt ccgggtcgtc caccagcgcc acgccgggga
     9481 aggcctcgat cgccgcgcga gccgcgtcca ggccgggctc cagctcga
//
LOCUS       DASPWO010000541         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_76453, whole genome shotgun sequence.
ACCESSION   DASPWO010000541 DASPWO010000000
VERSION     DASPWO010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76453"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..411)
                     /locus_tag="VEO00_12490"
     CDS             complement(40..411)
                     /locus_tag="VEO00_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYU58850.1"
                     /translation="MHAGIEATLRPFELSRPRFELLSRLLSRPEGEHLGRLGNLLFVH
                     PATITNLVDRLDAAGLIERRAAEGDRRAIVAKITPKGEQIARTAFRALADDQFALGHL
                     TADEVSTLRGLLVKLFADRPA"
     gene            676..>2057
                     /locus_tag="VEO00_12495"
     CDS             676..>2057
                     /locus_tag="VEO00_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYU58851.1"
                     /translation="MDLVRPPAPTTTRPYAVPTADVRLALRHVAGYERVEELPALRHA
                     ALDTVVEVVAEAGRLAVDRVLPLTRSGDAAHSRLVDGRVVPPPGFRSAYRAFVDGGWF
                     GVSAPEEHGGGGLPYVTSVALQEVLNACDPSFALCPVLTQGAMELLLAFGTPEQQARY
                     LPHLVAGTWTGTMNLTEPGAGSDVGALQTTAVRAEDGTWHISGTKIFITWGDHDLTEN
                     IVHLVLARTPGAPAGTRGVSCFLVPRRLIHPDGSLGDDNRLGPIGVEDKVGIRASPTC
                     TMAYDGAVGELVGDVGGGMRAMFVMMKRARLAMGAQGLGIAEAAVQLARTHAAERIQG
                     TPPLGTEDQDPMRRTIVDHPDIRRLLLACRAQVAALRGLVLLEAVELDLAQHHPDPSE
                     REAALALADILTPIVKAWGTELGVEGASAAMQVLGGMGYVEDTGAGQLWRDARIGPIY
                     EGTNGIQAIDL"
BASE COUNT          294 a          710 c          744 g          309 t
ORIGIN      
        1 tgtgatccga cggtatgtca aggaggatgc ccaggcgtgt tacgccggcc tgtcggcgaa
       61 cagtttgacg agcagccccc ggagggtgga cacctcgtcc gccgtcaagt ggccgagcgc
      121 gaactggtcg tccgccagtg cccggaacgc ggttcgggcg atctgctcgc ccttcggcgt
      181 gatcttggcg acgatcgctc ggcggtcccc ctcggcggcc cggcgctcta tgaggccggc
      241 cgcgtccaaa cggtcgacga ggttggtgat cgtcgccggg tgaacgaaca gcaggttccc
      301 gagccggccg aggtgctcgc cctcggggcg ggagagcagc cggctgagca gctcgaagcg
      361 cggcctcgaa agctcgaacg ggcgcagggt ggcttcgatg cccgcgtgca cgcttctgct
      421 cgcccgttcg agcgcttgcg ccagcgccgc tcgttcgggg gaccctggca gcccgatgga
      481 cttgaacgtc tcgcggatct ggtggatggg atcggcggac gactcagggg gggtcgcgtt
      541 ctttcgcgaa cgtgagctgt tcgtcgtccg tgctcgctgc gcagccatga cccgactgta
      601 gagccggcgg ctcaggcccc aacgaccgta ctgtgcaccg tagcagctcc atgagcggtc
      661 atctattatg tgtccatgga tctggtccgg cccccggctc cgacgaccac ccggccgtat
      721 gccgttccga cggccgacgt ccgcctcgcc ctccgccatg tagccggcta cgagcgcgtc
      781 gaggagctgc cagcgctccg gcacgccgct ctcgacacag tggtcgaggt cgttgccgag
      841 gcggggcgcc tggccgtcga ccgggtgcta ccgctgaccc gttccggcga cgcggcccac
      901 agccggctgg tcgacgggcg ggtcgtgccg ccaccggggt tccgcagcgc gtaccgggcg
      961 ttcgtggacg gcggatggtt cggtgtgtcc gcacccgagg agcacggggg cggcggcttg
     1021 ccgtacgtca cctcggtcgc cctgcaggag gtgttgaacg cgtgcgaccc ttcctttgcc
     1081 ttgtgcccgg tgctgactca aggcgccatg gagctgctcc tcgccttcgg cacgcccgag
     1141 cagcaggccc gctacctccc ccacctcgtc gccggcacgt ggacgggcac gatgaacctc
     1201 accgagccgg gcgccggatc ggatgtcggc gccctccaga cgactgccgt gagggccgag
     1261 gacggcacgt ggcacatctc gggcacgaag atcttcatca cgtggggcga ccacgacttg
     1321 accgagaaca tcgttcacct ggtcctcgcc cgcaccccag gcgccccggc ggggacacgg
     1381 ggagtcagct gcttcctggt gccccggcgt ctgatccacc ccgacgggag cctcggcgac
     1441 gacaaccggc tcggccccat cggagtcgag gacaaagtcg ggatccgcgc cagccccacg
     1501 tgcacgatgg cgtacgacgg cgccgtcggc gagctggtcg gcgacgtcgg cggcgggatg
     1561 cgggcgatgt tcgtcatgat gaagcgggcg cggctggcga tgggcgcgca gggcctggga
     1621 atcgccgagg ccgccgtcca gctggcgcgg acgcacgccg cggagcggat ccagggcacg
     1681 ccgccgctgg gcacagagga ccaagacccg atgcggcgga ccatcgtcga ccaccccgac
     1741 atccgtcgcc tcctcctggc ctgccgagcg caagtggcag cgctacgggg tctggtcctg
     1801 ctcgaagcgg tggagctgga cctcgcccag caccaccccg atccgtccga gcgcgaggcg
     1861 gcgctcgcgc tcgccgacat cctcacgccg atcgtcaagg cgtggggcac cgagctcggg
     1921 gtcgaggggg cgtcggcggc gatgcaggtg ctcgggggta tggggtacgt ggaggacacg
     1981 ggcgcgggcc agctgtggcg agatgcccga atcgggccca tctacgaggg gaccaacggg
     2041 atccaggcca tcgacct
//
LOCUS       DASPWO010000542         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_76572, whole genome shotgun sequence.
ACCESSION   DASPWO010000542 DASPWO010000000
VERSION     DASPWO010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76572"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VEO00_12500"
     CDS             complement(<1..438)
                     /locus_tag="VEO00_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYU58852.1"
                     /translation="MTDTDYVQLVERLDKEIAAVDGRGFVETPLERAGGLSDRLGFRP
                     IGGVWVKDETGNVSGSHKARHLFGLLVHLALVEEMGLIGDGSEIPDLAIASCGNAALA
                     AAVVARAGGRRLRVFVPTWADPWVLERLRSLGAVIEVCDRRPGV"
     gene            complement(663..2162)
                     /locus_tag="VEO00_12505"
     CDS             complement(663..2162)
                     /locus_tag="VEO00_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5'-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HYU58853.1"
                     /translation="MKRLGLERDLVDRDTYERTVARFREAEIVLPTFAELADPGKVPD
                     RIREALASIDPDAPHPLNLFRVHWYDDASRVGLVDVPEHVVLPKQLTGVDAKIVVAFG
                     NRFPMINAHKVLAAYGCLAPRVITGQFDPVNHRAIWPSTGNYCRGGVAISRIMQCHGV
                     AVLPEGMSQERFDWLDQWVLDPSDVIRTPGTESNVKEIYDKCAELAEDPANVIFNQFS
                     EFGNHLVHYLCTGAALERVFDSLRASNPELRLRAFTSATGSAGTIAAGDYLKERLGSL
                     VVAVEALECPTMLLNGFGEHNIQGIGDKHIPLIHNVMNTDVATAVSDRSTDQLGVLWN
                     TDVGREYLVDRRGVAGEVVDQLGDIGLSGICNILAAIKTAKYCDMGPDDVVMTVATDG
                     FAMYGSERDKAVGKYFPEGFDVVSAGEVFGEHLLGESTDNLRELTYEDRTRIFNLGYF
                     TWVEQQGVSVEEFRARKNPAFWTEIREIIPVWDRMIEEFNARTGAIEKL"
     gene            complement(2427..>2794)
                     /locus_tag="VEO00_12510"
     CDS             complement(2427..>2794)
                     /locus_tag="VEO00_12510"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYU58854.1"
                     /translation="GFHLHQGNDMFAAVGTPVVTPFDGRAVQTPNQLGGLAVTVYGAQ
                     GYVYNAHLAAYGKLGDVRSGDVVGFVGNTGDAAGGPWHDHFEWHPAHVPARAHVSPYG
                     VSDLAGAVDPFPYLLAACG"
BASE COUNT          443 a          992 c          902 g          457 t
ORIGIN      
        1 cacgccgggc cgccgatcgc acacttcgat caccgctccg agcgagcgca ggcgctccaa
       61 cacccacggg tcggcccagg tggggacgaa cacccgaagc ctgcgtccgc ccgccctcgc
      121 caccaccgcc gcagcgagcg cggcgttgcc acagctcgcg atggcgagat ccgggatctc
      181 ggacccatcc ccgatcaatc ccatctcctc gaccaatgcc aggtgcacca gcagcccgaa
      241 caggtggcgc gccttgtggg agcccgaaac gttgccggtc tcgtccttga cccacacgcc
      301 gccgataggg cggaaaccga gccgatcgct cagaccgccc gctcgctcca acggtgtctc
      361 gacgaaaccg cggccgtcca cggcggcgat ctccttgtcc agccgttcga ccaattggac
      421 gtaatccgtg tcggtcatgt cgagggagag ggcgagcgtg tgggaatgga acagctctcg
      481 gtagcggacg aacggattgg gctcgatccc gtgcggccac gagacctctc ggggatccaa
      541 ttgccgtatc agcacgtggt cggtgtcgtc cgttccagaa ttcggacatc ggaacgggta
      601 cgggtcgcgc ggggcggccc tccatccgca accgtgacag accagatcgg atccgtaccg
      661 gctcacagct tctctatcgc ccccgttcgc gcgttgaact cctcgatcat ccggtcccac
      721 acggggatga tctcgcggat ctcggtccag aacgccgggt tcttgcgggc ccggaactcc
      781 tcgacggaga cgccctgctg ctccacccag gtgaagtagc cgaggttgaa gatccgggtg
      841 cgatcctcgt aggtgagctc gcgcaggttg tcggtgctct cccccagcag gtgctccccg
      901 aacacctcgc ccgccgagac cacgtcgaac ccctcgggga agtacttccc gacggccttg
      961 tcgcgttccg atccgtacat ggcgaacccg tcggtggcca ccgtcatcac gacgtcgtca
     1021 gggcccatgt cgcagtactt cgccgtcttg atcgcggcca ggatgttgca gatgcccgac
     1081 aacccgatgt cgccgagctg gtccacgacc tcacccgcca cgcctcgacg gtcgacgagg
     1141 tactcgcgtc ccacgtccgt gttccacaac acgccgagct ggtcggtcga gcggtcggag
     1201 actgccgtgg ccacgtcggt gttcatcacg ttgtggatca acgggatgtg cttgtccccg
     1261 atcccctgga tgttgtgctc gccgaacccg ttcagcagca tcgtggggca ctccagcgcc
     1321 tccaccgcca cgaccagaga gccgaggcgc tccttcaggt agtcgcccgc ggcgatcgtt
     1381 ccggccgagc cggtggccga ggtgaatgcc cgaagcctca gttcggggtt cgatgcccga
     1441 agcgagtcga acacgcgctc cagcgccgca ccggtgcaca ggtagtgcac caagtggttc
     1501 ccgaactccg agaactggtt gaagatgacg ttcgccggat cctccgcgag ctccgcgcac
     1561 ttgtcgtaga tctccttcac gttggactcg gtgccggggg ttcggatgac gtccgaggga
     1621 tcgagcaccc attggtccag ccagtcgaac cgctcctggc tcatcccctc gggcaggacc
     1681 gccaccccgt ggcactgcat gatccgggag atggccacgc caccgcggca gtagttgccg
     1741 gtggacggcc agatggcccg gtggttgacc gggtcgaact gccccgtgat gacgcgtggc
     1801 gccaggcaac cgtaggccgc cagcaccttg tgggcgttga tcatggggaa gcggttcccg
     1861 aacgccacca cgatcttcgc gtcgacgccg gtcagctgct tcggcaggac cacgtgctcc
     1921 ggaacgtcga ccagcccgac gcggctcgcg tcgtcgtacc agtgcacccg gaagaggttc
     1981 agcgggtggg gggcgtccgg atcgatcgag gccagcgcct cccggatccg gtccgggacc
     2041 ttccccggat cggcgagctc ggcgaaggtc ggaagcacga tctccgcctc gcggaaccgg
     2101 gccaccgttc gttcgtaggt gtcccgatcg accaggtctc gctccagccc gagacgcttc
     2161 atcggagccg cctcatcgat cggggcgtgc cgcagcacgc gtcgtggccc ggcatcggta
     2221 ggcctccttt cgtccccccg ggcttcgtcc cgagggtagt catcgcgtgg tcgtgcgcgc
     2281 ttggttcagg ggccagtctg aacgtcgctc gggcctgatg tcgggacctt cggccgcaac
     2341 cgtccgggcc gtatgtccct gcccccgggg tggagtgtag gaccccggcg ccgccgcgcg
     2401 aggcgctcag cccggtggac cgccgatcac ccgcaggccg ccagcaggta cgggaacggg
     2461 tccacggcac cggcgaggtc ggaaaccccg tacgggctca cgtgggcccg ggccggcacg
     2521 tgggcggggt gccattcgaa atggtcgtgc cacggaccgc cggccgcgtc gccggtgttc
     2581 cccacgaacc cgaccacgtc gcccgacctc acgtcgccca gcttcccgta cgccgcgaga
     2641 tgggcgttgt acacgtaccc ctgggctccg tacacggtga cggccagccc acccagctgg
     2701 ttcggagtct gaaccgcgcg gccgtcgaac ggcgtcacga ccggcgtgcc gaccgcagcg
     2761 aacatgtcgt tcccctggtg gaggtggaat ccgc
//
LOCUS       DASPWO010000543         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1176903, whole genome shotgun sequence.
ACCESSION   DASPWO010000543 DASPWO010000000
VERSION     DASPWO010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176903"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..2991)
                     /locus_tag="VEO00_12515"
     CDS             complement(145..2991)
                     /locus_tag="VEO00_12515"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HYU58855.1"
                     /translation="MTATRRSRALLAIVGTAALLGAACKPAVLGTTGEPNVLQVSAAY
                     RSAVTPDAFSSRARDLTRALRRLREETHSGWIGRQDDQTGYLAELRGGTFPADGDPED
                     VAGTFFSSWALALFGIEAAELAFAPGEGTAGGPVVLRAEQRLHEVPVVDGVLTVTVAT
                     PEASPRVSAVRGRVFPGLQVSAEAAITAARAGRLAEQASGGAARDPTLVVLPVGASGA
                     LAWEVFVVGAQNAALPSALYYVSADDGAILAVRPASVQFSPFSTAASAAAVAAQATEG
                     EDVELTGQTPVGTAVTANGKRRGGKFLLIDTTTPTFDPATGRGGIETHDGINLEQEDL
                     PGPVSSSDTAEVADQEALGAHVYSREIFDYYNEVQGRASWDGQGATVTSSVHFGGPEF
                     CNAYFNGEQMVYGAPCEVAGEEITTFVDIDIAAHEVSHGVTATGSGLLYTGQSGAVNE
                     ALSDYFGNVIGNRFTGVDSVAIGEGGCVDIDHDTALCSPNPDGTRSIRYMLSGLQASD
                     YAFLLDPPISQKLLGLRQDNGGVHLNSQIFNNTLWEIRTRLAQLDGTDGNTSARAQAF
                     DHAVYVANTQIMTPTSSFLDTRAAIEQAARETGMDGESIRVIGQVFDLTGIVAANLSV
                     PPSPAQSVVATSAAQKAPQSGGDTIAWIDYPGGEGLTGQAAFVSADGGAAPTALGTAL
                     TATSVAFAGHSLLVADDPGIVTLYGPAGGAAQVLDPSVGVGSLILGLAGSGEGAAWLN
                     FENATLGFVDPSGTVTSAPVPPAATQSQPLSIGTGGGTVAVGSQGGNVIVWQAGGQPQ
                     LIQVPGGNPMAIDAFGDKLLVASFPGNLPIQGSGADLTCTVTLVDLTSGSQTVLSRNA
                     MPFGVTIGQDYAIWTQAVQRLSGGVIGNLPTGEGAFQDTDLWLYSLGTGRTYSVLTLR
                     GQQGYPSLEGDRLVWQEAAAGGDDVATGRLPSGL"
BASE COUNT          459 a         1185 c         1251 g          454 t
ORIGIN      
        1 tgggtatggg tggtgatcgc cgtgttcgcc gtggcggcag cgccagcacg gaggaactgc
       61 gataggccat ggtgcactac cggtcgctgt tcaatcagct gctggggagc gacgcagacg
      121 ttcgccgggc cggctgagcg aaggttagag gcctgagggg agccgcccgg tggcgacgtc
      181 atcgccgccg gcggcggcct cctgccacac caggcggtcg ccctccagcg aggggtagcc
      241 ctgctggccg cgcagcgtca ggacgctgta ggtcctcccg gtgcccaggc tgtacagcca
      301 caggtccgtg tcctggaacg cgccctcgcc ggtgggcagg ttcccgatca ccccgccgct
      361 caggcgctgc accgcctgcg tccagatcgc gtagtcctgg ccgatcgtca ccccgaacgg
      421 catggcgttc cgcgacagga ccgtctgcga gccgctcgtc agatccacca gcgtcaccgt
      481 gcaggtcagg tccgcgccgg acccctggat cgggagattc cccgggaagc tggcgacgag
      541 cagcttgtcg ccgaacgcgt cgatcgccat cggattgccg cccgggacct ggatcagctg
      601 gggctggccg cccgcctgcc agacgatgac gttcccgccc tgggagccaa ccgcgaccgt
      661 gccgcctcct gtcccgatcg agagcggctg ggactgggtc gcggcggggg ggacgggggc
      721 cgaggtcacg gtgccggacg gatcgacgaa cccgagcgtc gcgttctcga agttcaacca
      781 cgcggcgccc tcgcccgacc cggcgagccc gaggatcagc gaaccgaccc cgacgctcgg
      841 gtcgagcacc tgcgccgccc cgccggccgg cccgtagagc gtgacgatcc ccgggtcgtc
      901 cgccacgagc agcgagtggc cggcgaacgc caccgaggtg gccgtcagcg ccgtgccgag
      961 cgccgtcggc gcggcgccgc cgtcggcgga gacgaacgcg gcctgcccgg tgagtccctc
     1021 gcccccgggg tagtcgatcc aggcgatggt gtccccgccc gactgggggg ccttctgcgc
     1081 ggccgacgtg gccacaacgc tctgggccgg cgacgggggc acgctgaggt tcgcggcgac
     1141 gatgccggtc aggtcgaaca cctggccgat cacccggatc gactcgccgt ccatgccggt
     1201 ctcgcgggcc gcctgttcga tcgccgcgcg ggtgtcgagg aagctcgagg tcggcgtcat
     1261 gatctgggtg ttggcgacgt agaccgcgtg gtcgaaggcc tgcgcccgcg ccgacgtgtt
     1321 gccgtcggtc ccgtccagct gggccaatct cgtccggatc tcccacagcg tgttgttgaa
     1381 gatctggctg ttcaggtgga cgccgccgtt gtcctgccgc aggccgagca gcttctgcga
     1441 gatcggcggg tcgagcagga acgcgtagtc gctcgcctgc aggcccgaca gcatgtagcg
     1501 gatcgagcgg gtcccgtccg ggttcgggct gcacagggcc gtgtcgtggt cgatgtcgac
     1561 gcagccgccc tcgccgatcg ccacgctgtc cacgccggtg aaccggttcc cgatcacgtt
     1621 gccaaagtag tccgagagcg cctcgttcac cgcccccgac tggccggtgt acagcaggcc
     1681 gctgccggtc gcggtgacgc cgtgggagac ctcgtgggcg gcgatgtcga tgtcgacgaa
     1741 cgtggtgatc tcctcgcccg ccacctcgca cggcgcgccg tagaccatct gctcgccgtt
     1801 gaagtaggcg ttgcagaact cggggccgcc gaagtggacc gacgaggtga ccgtggcgcc
     1861 ctgcccgtcc caggacgccc tcccctgcac ctcgttgtag tagtcgaaga tctcccggct
     1921 gtagacgtgc gccccgagcg cctcctggtc ggcgacctcg gcggtatccg aggaggagac
     1981 gggccccggg aggtcttcct gctccaagtt gatgccgtcg tgggtctcga tcccgccgcg
     2041 cccggtggcc gggtcgaagg tgggcgtcgt ggtgtcgatc agcaggaact tcccgccccg
     2101 gcgcttgccg ttcgcggtga cggccgtgcc caccggggtc tggccggtca gctcgacatc
     2161 ctccccctcg gtggcctgcg cggcgacggc ggcggcggac gcggcggtgg agaagggcga
     2221 gaactgcacc gaggccgggc gcacggcgag gatggcgccg tcgtcggcgg agacgtagta
     2281 cagcgccgag ggcagggcgg cgttctgcgc tccgacaacg aacacctccc aggcgagcgc
     2341 gccggaggcc ccgaccggga ggaccacgag cgtcgggtcc cgggccgccc ccccggaggc
     2401 ctgctcggcg agcctgccgg ccctggcggc ggtgatggcg gcctcggcgg agacctgcag
     2461 gccggggaac acccggccgc ggacggccga gacccgcggg gaggcctccg gggtggcgac
     2521 ggtcaccgtc agcacgccgt cgaccaccgg gacctcgtgc aggcgctgct cggcgcggag
     2581 caccaccggg ccgccggcgg tgccctcccc cggggcgaag gcgagctccg ccgcctcgat
     2641 cccgaacagg gcgagggccc aggaggagaa gaaggtcccg gccacgtcct cgggatcccc
     2701 gtccgccggg aaggtccccc cgcgcagctc ggcgaggtag ccggtctggt cgtcctggcg
     2761 cccgatccag ccggagtggg tctcctcgcg cagccggcgc agggcccggg tcaggtcccg
     2821 ggcgcgcgaa ctgaacgcgt cgggggtgac cgccgagcgg taggccgccg agacctggag
     2881 cacgttcggc tccccggtgg tgccgagcac ggccggcttg caggccgccc cgagcagggc
     2941 cgccgtcccc acgatcgcga ggagagcgcg cgagcggcga gtcgcggtca cggagcccga
     3001 gtatacgcgg ggggcttcgc tccaggcccc cggggaggag ccccgtgaac acgtcgtcgc
     3061 gccaccgccg gcgcccggtg gaccggtcat tccgcgcggt tgacgcgcgc tggacggggg
     3121 caaagaatcg aggacggtgc cccggggtgg atgacccgac agtccccgga gcgagaaggg
     3181 gggacgggat gcagtgcccg acgtgcaacg ctgagaatcc cagcgaggcc agctactgct
     3241 ggcggtgcgg acaggcctta cgccgcgccg acgcctcgaa gggtggacgg cggtccgcct
     3301 acgcggtcca gtcgtcggag aacgtgacgc agttcgcgct catctcgac
//
LOCUS       DASPWO010000544         5595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1096724, whole genome shotgun sequence.
ACCESSION   DASPWO010000544 DASPWO010000000
VERSION     DASPWO010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096724"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="VEO00_12520"
     CDS             complement(<1..1623)
                     /locus_tag="VEO00_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58856.1"
                     /translation="MFQTDPRTPRASRMLAAALTLLAAACAPKLSALAPGSTSPKGLR
                     FVPEVVDIRLNAGPGLSVAVSSDGTPDASYFRLDEEVALPLQPVPEDPTALPAVMVAT
                     RVEGGLWVRQVVLQEREEPAEGEQASGPKKLDGLDPETDATDIAVDGNGARHVVWTVA
                     NGTVQYATDAGGAFPTGDAGEAETVAEGSAVSGAAVAVAEDGTPWVSYYEGTSVRAAS
                     RSGTTWTVEEIGPASAPGPVAGEPAPPAAGQRTDVAAGPDGPIVVYGDGDATMVATRG
                     TDGTWTTEEADGDGGFGAAIALDADGNPHLAYYSAAGDVRHAHRTGAAWSANVVSKSA
                     AAELDARTTASIALSGDGTHWVAFARTAKKASTVWIAENSGGDFAAKAMPDGAGGARP
                     AIAAVGDHLAAAWYDTVTTSLLIAPQTNRPPPVALPSPPAGTPGASPKTSPTATGSPT
                     GGGNLPCEPNGTDLQISAQNIQFDTDCLAAPAGKAFTVTFTNKDEGVPHNWEAFTDQS
                     ASQRIGGATDASDTITTGSVTYDVDALDAGQYY"
     gene            complement(1635..4493)
                     /gene="uvrA"
                     /locus_tag="VEO00_12525"
     CDS             complement(1635..4493)
                     /gene="uvrA"
                     /locus_tag="VEO00_12525"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYU58857.1"
                     /translation="MATGSIVVRGAREHNLKNLHLELPRDKLIVFTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARQFLGQMEKPDVDFIEGLSPAISIDQKSSSKNPRSTVGT
                     ITEIYDYLRVLYARIGRMHCPKCGRPIGRQTTDQIVDRILELPEGTRFQVLGPVVRGR
                     KGEYERLLSDLAKRGFARARVDGKPVELTGTIRLDRYYKHDIEIVVDRLVAKEGIRRR
                     VADAVETATQLAEGIVAVAVQREGGREEIQTYSQQYACVFDGLSFEEPAPRNFSFNSP
                     YGACPTCDGLGTRLEVDPSAIVPDPDLSIAEGAIAPWGTSRLEYWQRLLEAVGEAYGF
                     DIRTPWRRLSKKARDVILYGSGDTEVYVKYRNRYGRVRSYYTSFQGVIPVTERRHAEA
                     ESDLARQRLEQYMREVPCRACRGMRLKPESQAVTVGGLNISELSANSVKDLAAFLDGL
                     VLDARETLIAERLLKEIRARLGFLLDVGLDYLTIDRASATLAGGEAQRIRLATQIGSG
                     LVGVLYILDEPSIGLHQRDNKRLLDTLIRLRDLGNTLIVVEHDEATIRAADHVVDIGP
                     GAGELGGEIVVSGTLEDVLAHPRSLTGAYLTGRRSIPLPRARRLPGDRWLTVVGAREH
                     NLRGIDVAFPLGCLISVTGVSGSGKSTLVEDILYRGLQSKVHRSRVVPGKHRRLAGWQ
                     AVDKVINIDQSPIGRTPRSNPATYIGVFDHIRKLFAQVPEARVRGYQPGRFSFNVRGG
                     RCEGCAGDGQIKIEMHFLPDVYVTCEVCKGQRYNRETLEVRYKGRSIADVLAMSVDEA
                     LEFFGRIPVIARHLQTMSDVGLGYVRLGQPAPTLSGGEAQRVKLASELSRRATGNTFY
                     ILDEPTTGLHFDDVRKLLSVLDRLVDAGNTVVVIEHNLDVVKTADWIIDLGPEGGDRG
                     GEVVAAGPPETVASNPRSYTGAVLAEVLEPHRVAAVAG"
     gene            complement(4445..4948)
                     /locus_tag="VEO00_12530"
     CDS             complement(4445..4948)
                     /locus_tag="VEO00_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58858.1"
                     /translation="MEGRDAPGGAATAARRPWAAVGAAGLALAIACLVLGAGYALTPA
                     TGGDGARRFAPWDPALDPWVGLAGVAGGTLIALTSVALARRLRGRGSSAGVGGAATVV
                     AAFAVADALGLWLWATAEPPLPPSWAWLRPVHWAGLALAGVWAVVTTMAAWPRDRSSS
                     AGPVSTT"
     gene            4967..>5595
                     /locus_tag="VEO00_12535"
     CDS             4967..>5595
                     /locus_tag="VEO00_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYU58859.1"
                     /translation="MDLEGYREEFPVLARKAYLISASLGPVSRRSLAYLDEYVTAWTE
                     LGSPEPVWFERIFPRLREVKRSFARLTGAEPDELAITTNVSLALSTIASCLDFPPQRN
                     RVIISELDFPTEFHVWRAQERRGADVVVLPSPDGLTVPVEAYARAIDDRTAVACVNRV
                     LYRSSALLDATAICRLAAEAGAVSVVDDFHGAGIVPVDVRDLGCDFYTTG"
BASE COUNT          735 a         2053 c         1999 g          808 t
ORIGIN      
        1 gtagtactgg ccggcatcca gagcgtcgac gtcgtaggtc accgacccgg tggtgatcgt
       61 gtcgctggca tcggtcgcgc ccccgatccg ctgcgaggcg gactggtcgg tgaacgcctc
      121 ccagttgtgc gggacgccct cgtccttgtt ggtgaacgtc acggtgaagg ccttccccgc
      181 cggggcggcc aggcagtcgg tgtcgaactg gatgttctgc gccgagatct gcaggtcggt
      241 gccgttcggc tcgcagggca ggttcccccc gccggtcggg ctccccgtgg cggtcggcga
      301 cgtcttcggg gaggcgccgg gggtgccggc cggcggggac ggcagcgcca ccggcggggg
      361 ccggttcgtc tgcggcgcga tgagcagcga ggtcgtcacc gtgtcgtacc aggcggccgc
      421 caggtggtcg ccgacggcgg cgatcgccgg ccgagcgccg ccggcgccgt ccggcatggc
      481 cttcgcggcg aagtcgccgc cggagttctc ggcgatccag accgtgctgg ccttcttcgc
      541 cgtccgggcg aacgccaccc agtgcgtccc gtccccgctc agggcgatcg aggcggtggt
      601 ccgggcgtcc agctccgccg cggcgctctt ggacacgacg ttggcggacc aggccgcgcc
      661 cgtgcggtgg gcgtggcgga cgtcgccggc cgccgagtag tacgccaggt gcgggttccc
      721 gtcggcgtcg agcgcgatcg ccgcgccgaa gcccccgtcg ccatcggcct cctcggtggt
      781 ccaggtcccg tccgtgcctc gcgtggcgac catcgtcgcg tcgccgtcgc cgtagacgac
      841 gatcgggccg tccgggcctg cggcgacgtc cgtccgctgg cccgcggcgg ggggtgccgg
      901 ttcgccggcg acgggcccgg gagcggacgc cggcccgatc tcctccacgg tccaggtcgt
      961 tccggagcgc gaggccgccc gcaccgacgt gccctcgtag tacgagaccc acggggtgcc
     1021 gtcctcggcg accgccaccg ccgccccgga gaccgcggaa ccctcggcca cggtctcggc
     1081 ctcgcccgcg tcgccggtgg ggaacgcgcc gccggcgtcg gtggcgtact gcaccgtgcc
     1141 gttcgcgacc gtccacacca cgtggcgcgc gccgttcccg tccacggcga tgtcggtggc
     1201 gtcggtctcg ggatcgagcc cgtcgagctt cttcgggccg ctggcctgct cgccctcggc
     1261 cggctcctcg cgctcctgga gcaccacctg ccgcacccag agcccgccct cgacccgcgt
     1321 cgccaccatc accgccggca gcgcggtggg gtcctctggc accggctgca gcggcagggc
     1381 cacctcctcg tcgagccgga agtacgaggc atccggggtg ccgtccgacg acacggccac
     1441 ggagagcccg ggtccggcgt tcagacggat gtcaaccact tcgggcacga accgcagccc
     1501 cttcggggag gtgctgcccg gggcgagcgc cgagagcttc ggcgcgcagg ccgccgccag
     1561 cagggtcagg gcggcggcga gcatccggct ggcgcgcggc gtgcgcgggt cggtctggaa
     1621 cacgaacggg gattctagcc cgcgaccgcc gcgacgcggt ggggctcaag cacctccgcc
     1681 aggaccgcgc cggtgtagga gcgcgggttg gacgccaccg tctcgggcgg tccggcggcc
     1741 acgacctcgc cgccgcggtc cccaccctcg gggccgaggt cgatgatcca gtccgccgtc
     1801 ttgaccacgt ccaggttgtg ctcgatgacc accaccgtgt tcccggcgtc caccaggcgg
     1861 tcgagcaccg agagcagctt gcgcacgtcg tcgaagtgca gcccggtcgt cggctcgtcg
     1921 aggatgtaga aggtgttccc ggtcgcccgc cgggacagct cggaggcgag cttgacccgc
     1981 tgggcctcgc cgccggagag cgtcggcgcc ggctggccga gccggacgta cccgagcccg
     2041 acgtcggaca tcgtctgcag gtgacgggcg atcaccggga tcctgccgaa gaactcgagc
     2101 gcctcgtcga cgctcatggc cagcacgtcg gcgatcgagc ggcccttgta gcgcacctcc
     2161 agggtctcgc ggttgtagcg ctggcccttg cacacctcgc aggtgacgta gacgtccggc
     2221 aggaagtgca tctcgatctt aatctggccg tctccggcgc acccctcgca gcggcctccg
     2281 cgaacgttga acgagaacct ccccggctga tacccgcgca cccgcgcctc cggcacctgc
     2341 gcgaacagct tccggatgtg gtcgaacacg ccgatgtacg tcgcggggtt ggagcgcggg
     2401 gtgcggccga tcggcgactg gtcgatgttg atgaccttgt cgaccgcttg ccacccggcc
     2461 agcctgcggt gcttgccggg gaccacccgc gagcggtgca ccttcgactg cagcccgcgg
     2521 tacaggatgt cctcgacgag cgtcgacttc ccggaccccg acacccctgt caccgagatc
     2581 aggcagccga ggggaaaggc gacgtcgatg ccgcgcaggt tgtgctcgcg ggcgccgacg
     2641 acggtcagcc agcggtcgcc gggcaggcgg cgcgcccgcg ggagggggat cgagcggcgg
     2701 ccggtcaggt aggcgccggt cagcgaccgg gggtgggcca gcacgtcctc gagagtcccg
     2761 gagacgacga tctcgcctcc cagctcgccc gcgcccgggc cgatgtcgac gacgtggtcg
     2821 gcggcccgga tcgtggcctc gtcgtgctcc acgacgatca gcgtgttccc caggtcgcgc
     2881 agccggatca gcgtgtcgag gagccgcttg ttgtcgcgct ggtgcagccc gatcgacggc
     2941 tcgtcgagga tgtagagcac cccgaccagc cccgacccga tctgcgtcgc cagccggatg
     3001 cgctgcgctt cgccgccggc gagcgtcgcc gaggcgcggt cgatcgtcag gtaatccagg
     3061 ccgacgtcca gcaggaaccc cagacgcgcg cggatctctt tcagcagccg ctcggcgatc
     3121 aacgtctcgc gcgcgtccag gaccaggccg tcgaggaagg ccgccaggtc cttcaccgag
     3181 ttggcggaca gctccgagat gttcagtccg ccgacggtca ccgcctggct ctccggcttc
     3241 aggcgcatgc cccggcacgc tctgcacgga acctcgcgca tgtactgctc caggcgctgg
     3301 cgcgccaggt ccgattcggc ttctgcgtgc cggcgctcgg tgaccgggat gaccccctgg
     3361 aaggacgtgt agtaggaacg cactcgcccg taacggttgc ggtacttcac gtacacctcg
     3421 gtgtcgcccg agccgtacag gatgacgtcg cgcgccttct tcgagagcct gcgccacggc
     3481 gtgcggatgt cgaacccgta cgcctccccg accgcctcca gcaggcgctg ccagtactcc
     3541 aggcggctcg tcccccaggg ggcgatggct ccctcggcga tcgacaggtc cgggtcgggg
     3601 acgatcgccg aggggtcgac ctccaggcgc gtcccgagcc cgtcgcacgt cgggcaggcg
     3661 ccgtacggcg agttgaacga gaagttccgc ggggccggtt cctcgaacga cagaccgtcg
     3721 aagacgcagg cgtactgctg cgagtaggtc tggatctcct cccggccgcc ctcacgctgg
     3781 acggcgaccg cgacgatgcc ctccgcgagc tgcgtggcgg tctcgaccgc gtcggcgacg
     3841 cgccggcgga tgccctcctt cgccacgagc cggtccacga cgatctcgat gtcgtgcttg
     3901 tagtaccggt cgagccggat cgtcccggtc agctcgaccg gcttcccgtc gacccgcgcc
     3961 cgggcgaacc ctcgcttcgc caggtcggac agcaggcgct cgtactcgcc cttccttccg
     4021 cgaaccaccg gtccgagcac ctggaaccgc gtcccctccg gcagctccag gatgcggtcc
     4081 acgatctggt cggtggtctg ccggccgatc ggtcgtccgc acttcgggca atgcatccgg
     4141 ccgatgcgcg cgtagagcac ccggaggtag tcgtagatct cggtgatcgt cccgaccgtc
     4201 gagcgcgggt tcttcgacga cgacttctgg tcgatcgaga tcgccggcga gagcccctcg
     4261 atgaagtcga cgtcgggctt ctccatctgc cccaggaact gccgggcata ggaggacagc
     4321 gactccacgt acctccgctg gccctcggcg tagatcgtgt cgaaggcgag cgaggacttc
     4381 ccggagccgg agagtcccgt gaacacgatc agcttgtcgc ggggaagctc cagatggagg
     4441 ttcttcaggt tgtgctcacg ggccccgcgg acgacgatcg atcccgtggc cacgcggcca
     4501 ttgtagtgac cactgcccac acgcccgcca gcgccagccc ggcccagtgc accggccgca
     4561 gccaggccca cgacgggggc aggggcggct ccgcggtggc ccacagccag agcccgagcg
     4621 cgtcggcgac ggcgaacgcc gcgacgacgg tcgccgcgcc gcccacgccc gccgacgacc
     4681 cccgcccccg cagccgccgg gcgagggcca ccgacgtcag cgcgatcagc gtcccaccgg
     4741 ccacaccggc gagcccgacc cacgggtcga gcgccgggtc ccacggcgcg aagcgtcgtg
     4801 ccccgtctcc ccctgtcgcg ggggtgaggg cgtacccggc gccgaggacc aggcaagcga
     4861 tcgccagcgc cagcccggcc gcgccgacgg cggcccacgg ccgtcgagcg gccgtcgcgg
     4921 cgccgccggg tgcatcccgg ccttccacgc ggtctagggt agcgacgtgg acctggaggg
     4981 ctaccgggaa gagttcccgg tgctggcgcg caaggcgtac ctgatcagcg cctcgctcgg
     5041 cccggtgtca cgccgctcgc tggcgtacct ggacgagtac gtgaccgcct ggaccgagct
     5101 cggttcgccc gagccggtgt ggttcgagcg gatcttcccg cgattgcgcg aggtgaagcg
     5161 gtcgttcgcc cggctgaccg gggccgagcc ggacgagctg gcgatcacga cgaacgtgtc
     5221 gctggcgctc tccactatcg cgtcctgcct ggacttcccg ccgcagcgca accgggtgat
     5281 catctccgag ctcgacttcc cgaccgagtt ccacgtctgg cgggcgcagg agcgccgcgg
     5341 cgcggacgtg gtcgtgctgc cgagccccga cgggctgacc gtcccggtgg aggcgtacgc
     5401 gcgggcgatc gacgaccgga cggcggtcgc gtgcgtgaac cgggtcctgt atcgctccag
     5461 cgcgctgctc gacgcgacgg ccatctgccg gctggcggcg gaggcgggcg cggtctccgt
     5521 ggtcgacgac ttccacggcg cggggatcgt cccggtggac gtgcgcgacc tggggtgcga
     5581 cttctacacc accgg
//
LOCUS       DASPWO010000545         4619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1007115, whole genome shotgun sequence.
ACCESSION   DASPWO010000545 DASPWO010000000
VERSION     DASPWO010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007115"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..532)
                     /locus_tag="VEO00_12540"
     CDS             complement(116..532)
                     /locus_tag="VEO00_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HYU58860.1"
                     /translation="MQASGGEPQRPVGESLRKIRRSRDDRVIAGVAGGIGHHLGVDPL
                     LIRIAFVVLTLSGGSGILLYVLGWIFIPEERTPGEVTAAPPERAGAVAVIVGTALIAL
                     GSLLLLSRIFPDFNRIVGPIVLIAIGVGVVVTASRR"
     gene            582..1964
                     /locus_tag="VEO00_12545"
     CDS             582..1964
                     /locus_tag="VEO00_12545"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYU58861.1"
                     /translation="MVPEEAVRAAIDSLVAWQPVTNACSQLWPDEAIEAARGLPVEPG
                     GPLHGVPVLVKDLYDVAGHETTGCSAAFRGNVAASDAPLVTALRAAGAVIVGKANMHE
                     LAAGGTNHVSACGRTRNPWDPERITGGSSGGSAAAVAQRSVPLSLGSDTGGSIRIPSS
                     LCGVTGLKPTHGRLPLDGVMPLAPSLDCPGPIAATAEGAALAFAVLAGEAHEPAPASA
                     YRVGVVAEGFFAERMTTAVRGALEEAGRTLGAAGAVVVEAALPGLEDGPHVWTDLAWP
                     EFADTYPDLDLERLLPETAFLHWYGRRVTPAERADARRRRTKIRATFERALESVDAIL
                     LPATPFPAPRFDEEHVPVEGGGFGGVARPGERSARPRVASEPPKEGGDTLDLYQGGPG
                     WFTRPVSLTGLPSLAFPVGFEENLGLPLGAQLVGRANGEWVLLDLARAFQAVTGHHLR
                     APAAPPPPGS"
     gene            complement(2023..2151)
                     /locus_tag="VEO00_12550"
                     /pseudo
     CDS             complement(2023..2151)
                     /locus_tag="VEO00_12550"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2247..2606)
                     /locus_tag="VEO00_12555"
                     /pseudo
     CDS             complement(2247..2606)
                     /locus_tag="VEO00_12555"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            2659..3561
                     /locus_tag="VEO00_12560"
     CDS             2659..3561
                     /locus_tag="VEO00_12560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYU58862.1"
                     /translation="MSRRDDLIGGAYAAIASLAFGAVAVVGKIVTDRGMTVTSLLGVR
                     FGVAAIVLALVQGVRRGPLRPARGEWWRLLLLGVAGYALESGLFFAALRHGTVSAVTL
                     LFFTYPVFVAIGSVLLGRGLPGWVVVGSLVVALAGAATVAASAGGVDISALGVLFALL
                     ASFAFAVYLVAAEALLERTPPQVGATWVAGAASLGLFTAALVSRTGDLPPSGAKGWVM
                     LGFMGAGTALAFTGLFGAVQRLGAVRTAVVSALEPATTALMGVAFLGDPLRTGTVVGG
                     VLILVAAFAASVARGIPTAEAPVA"
     gene            complement(3576..>4619)
                     /locus_tag="VEO00_12565"
     CDS             complement(3576..>4619)
                     /locus_tag="VEO00_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HYU58863.1"
                     /translation="LGYSGTNAGGDLWVVNSIWRNNMGGIVPNTLDTERLAPQRGAVV
                     AGNLVYDNNNLDAPAKSIAYPSLGIGILVAGGRDNVVRDNRVSGHRHYGIAVVPNVDE
                     NVWFSGRNRVVRNRVDRSGIADLAVGGPAMGGNCFADNAFETSLPIAIELTAGCGGAL
                     NRLGGGDLSVSLHALSLFADAGDGEFPHGDWTVQRAPPDQLEMPGDLETWTPGASMPG
                     PRPVIRVPEAPDGALGPDPGSAVSVLGVPFVGATPWELLLGLYGYVLPLVLYAAWVSV
                     ALWDLVRRDGLRSGARLGWLAVVLIVPFLGPLIYLAFGKSEIPRSVRIMLVAGSLAAY
                     AAFTLLVYLLASI"
BASE COUNT          583 a         1683 c         1750 g          603 t
ORIGIN      
        1 acgtcgacga catcggtggt ctcgagcagc cagccgacac cgagcagcag cagcaggacc
       61 ccggcggcga tccgcccgag cgggaacggc ctggacgggc ggtcctccgt gccgttcatc
      121 gccggctggc cgtcacgacc accccgaccc cgatggcgat caggacgatc ggcccgacga
      181 tccggttgaa gtccgggaag atccggctca ggagcaggag tgagccgagg gcgatcaggg
      241 cggtgccgac gatgacggcc acggcgccgg cgcgctccgg cggtgccgcc gtgacctcac
      301 ccggcgtcct ctcctcgggg atgaagatcc accccaggac gtagagcagg atcccggagc
      361 cgccggacag cgtcagcacc acgaacgcga tccggatcag gagtgggtcg acgccgaggt
      421 gatggccgat cccgccggcc acgcccgcga tgacccggtc gtcgcggctg cggcggatct
      481 tccgaaggct ctcgccgacc gggcgctggg gctcccctcc cgacgcctgc atgacggcgc
      541 cagcgtaccg caccgtgccc gtccgatacc ctccgagcgg aatggtcccc gaggaggccg
      601 tgcgggctgc gatcgattcg ctcgtggcgt ggcagccggt gacgaacgcc tgctcgcagc
      661 tgtggccgga cgaggcgatc gaggcggcgc gcggactgcc cgtcgagccg ggcgggccgc
      721 tgcacggcgt cccggtactg gtcaaggacc tgtacgacgt ggccgggcac gagaccaccg
      781 gctgcagcgc cgcgttccgc ggcaacgtcg cggcatcgga cgcgccactg gtgacggcgt
      841 tgcgagcggc cggcgccgtg atcgtcggca aggcgaacat gcacgagctg gccgccgggg
      901 ggacgaacca cgtctcggcc tgcggccgga ccaggaaccc ctgggacccg gaacggatca
      961 ccggcggctc gagcggcggc tcggccgccg ccgtcgcgca gcgctcggtc ccgctctcgc
     1021 tcgggagcga cacaggaggc tcgatccgca tcccgtcctc gctctgcggg gtcaccggac
     1081 tgaagccaac gcacgggcgg ctgccgctcg acggcgtcat gccgctcgct ccctcgctcg
     1141 actgcccggg cccgatcgcc gcgacggccg agggggcggc gcttgcgttc gccgtgctgg
     1201 ccggcgaggc gcacgagccc gcgccggcct ccgcctaccg cgtcggcgtg gtcgccgagg
     1261 ggttcttcgc cgagcggatg acgaccgccg tccggggggc gctcgaggag gcagggcgga
     1321 cactcggggc ggccggggcc gtcgtggtcg aggcggcgct gcccggcctg gaggacgggc
     1381 cgcacgtctg gaccgacctg gcctggccgg agttcgccga cacgtacccc gacctcgacc
     1441 tcgagcgcct gctgccggag accgcgttcc tgcattggta cgggcgccgc gtcacaccgg
     1501 ccgagcgcgc cgacgcgcgc cgccggcgca cgaagatccg cgccacgttc gagcgcgcgc
     1561 tggagagcgt cgacgcgatc ctgctcccgg cgacgccgtt ccccgccccg cggttcgacg
     1621 aggagcacgt cccggtggag ggcggggggt ttgggggggt agctcgccct ggggagagat
     1681 ctgcccgacc acgtgtggcg agcgaacccc ctaaggaggg aggcgatacg ctcgatctgt
     1741 accagggagg gccgggatgg ttcacccggc cggtcagcct gaccggcctg ccgtccctgg
     1801 cgttcccggt cggcttcgag gagaacctcg gccttccgct cggggcgcag ctcgtcgggc
     1861 gggcgaacgg cgagtgggtg ctgctcgacc tcgcccgggc gttccaggcg gtgaccggcc
     1921 accacctgcg agccccagcc gcgccgccac cgccgggctc ctgagctcgc gcagcgcgtc
     1981 agcggcgatc caccgcgccg cgcgcgagcc gctcgcggcg aggccctcgg cggtgcccac
     2041 ggcccgggcg tgcagcgcgg cgttgcgctt tccgatctgc cgcagcgccc agttgacggc
     2101 cttcttgacg taggtgcgct cgtccttcgc gccacgccgg atcgctgcca ggaaccgagc
     2161 gaggcgcggt cgggcgcggc ccggtcgtgg acggcgagca cggccatcag cgcgtagccg
     2221 gcgcgcttga cgaactcctc atcgccgccg gcccagcgca gcgcggcggc gtgcgcgaag
     2281 ggcgaacgga cgaagaggtt caggcaggcc tggtcgcaca agtcccacga gtcgaacgag
     2341 cgcgcccagc ggtcggcgac cgggggcgtg acccgtgccg ggtcggcgac catggtcgcc
     2401 agcaggcgcg cctcgtgcac ccccgaccgc cacaggcgca gcgccagcgc gtgatcggtg
     2461 ccgatctcgc gcgccaggcg gcgcaggtcg gtgaccgagg cgccgacgac ctcgccgcgc
     2521 gggcggatcc cgaaccgggc catcccctcg gcgccgcccg gtcgcgccag cgcgcggatc
     2581 cgccggatcg ccacccgctc gtccatcgcg gcggatcgta cgcggcccgt cagggaggct
     2641 ccgctagcat ccgtccccgt gtcccggcgc gacgacctga tcggcggcgc ctacgccgcg
     2701 atcgcctcgc tggcgttcgg cgccgtggcg gtggtcggaa agatcgtcac cgaccgcggg
     2761 atgaccgtga cgagcctgct gggcgtccgg ttcggcgtgg cggcgatcgt gctggcgctg
     2821 gtccaggggg tccggcgcgg gccgctgcgg ccggcgaggg gcgagtggtg gcggctgctg
     2881 ctgctcgggg tcgccgggta cgcgctcgag tcgggcctgt tcttcgcggc gctgcggcac
     2941 gggaccgtct ccgcggtgac gctgctgttc ttcacgtacc cggtgttcgt ggccatcggc
     3001 tcggtcctgc tcggacgcgg gctgcccggg tgggtggtgg tcgggtcgct cgtggtggcg
     3061 ctcgccggcg cggccacggt ggccgcctcg gccggcgggg tggacatcag cgcccttggc
     3121 gtgctgttcg cgctcctggc gtcgttcgcg ttcgcggtct acttggtcgc cgccgaggcc
     3181 ctgctggagc ggacgccgcc ccaggtcggc gcgacctggg tggcgggggc ggcgtcgctc
     3241 gggctcttca ccgccgcgct cgtctcgcgg accggagacc tcccgccgag cggcgcgaag
     3301 ggatgggtga tgctcgggtt catgggcgcc ggcacggcgc tggcgttcac cggcctgttc
     3361 ggcgcggtgc agcggctggg cgccgtgcgg accgcggtcg tctccgccct cgagccggcg
     3421 accaccgccc tgatgggcgt ggccttcctc ggggacccgc tgcggacggg cacggtcgtc
     3481 ggcggcgtcc tgatcctggt ggcggccttc gccgcgagcg tcgcccgcgg catccccacc
     3541 gccgaggccc cggtggcctg accggggacg gtcgatcaga tcgacgccag tagatagacc
     3601 agcagcgtga aggcggcgta agcggccagg ctcccggcga ccagcatgat ccgcacgctg
     3661 cgcgggatct ccgacttccc gaacgcgagg tagatcaggg ggccgaggaa gggcacgatc
     3721 agcaccaccg ccagccaccc gagtcgcgcg cccgagcgca ggccgtcccg gcggaccagg
     3781 tcccacagcg ccaccgatac ccaggcggcg tagagcacga gcgggagcac gtagccgtag
     3841 agcccgagta gcagctccca cggcgtcgct ccgacgaacg ggacgccgag cacggacacc
     3901 gccgatcccg gatcgggccc gagcgcgccg tccggcgcct ccggcacgcg gatgaccggc
     3961 cgcggcccgg gcatgctcgc gcccggcgtc cacgtctcga ggtcgcccgg catctcgagc
     4021 tggtcgggcg gggcgcgctg gaccgtccag tccccgtggg ggaactcgcc gtcgccggcg
     4081 tcggcgaaca gggacagcgc gtgcagcgag accgacaggt cgccgccacc gagccggttc
     4141 agcgcgccgc cgcatccggc ggtcagctcg atcgcgatcg gcaggctggt ctcgaaggcg
     4201 ttgtccgcga agcagttccc gcccatcgcc ggtccgccca cggccaggtc ggcgatccct
     4261 gaacggtcga cccggttgcg caccacgcgg ttcctgccgg agaaccagac gttctcgtcg
     4321 acgttcggga ccaccgcgat cccgtagtgg cggtgtcccg acacgcggtt gtcgcgcacg
     4381 acgttgtcgc gcccgccggc gaccagaatg ccgatgccga gggagggata ggcgatcgac
     4441 ttggccgggg cgtccaggtt gttgttgtcg tagacgaggt tcccggcgac gaccgcgccc
     4501 cgctgcggag cgaggcgctc ggtgtcgagg gtgttcggga cgatcccgcc catgttgttc
     4561 cgccagatcg agttcacgac ccagagatcg ccgccggcgt tggtgccgga gtagccgag
//
LOCUS       DASPWO010000546         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1260812, whole genome shotgun sequence.
ACCESSION   DASPWO010000546 DASPWO010000000
VERSION     DASPWO010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260812"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VEO00_12570"
     CDS             complement(<1..249)
                     /locus_tag="VEO00_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HYU58864.1"
                     /translation="MRSLQHPCLCPAPRSAFAGFRFPPEVITLAVRWYLHFGLSYRDV
                     EELLAERGIEVDHVSIYRWVQRFAPEFAEAARARQHVIG"
     gene            422..544
                     /locus_tag="VEO00_12575"
     CDS             422..544
                     /locus_tag="VEO00_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58865.1"
                     /translation="MEAVPHLRRGIVALRQYAERHGVPDELIRRAEARHRSRHA"
     gene            806..>2251
                     /locus_tag="VEO00_12580"
     CDS             806..>2251
                     /locus_tag="VEO00_12580"
                     /inference="COORDINATES: protein motif:HMM:NF023683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58866.1"
                     /translation="MKARIILILSAAICLAFAAAAPAALADGVEPRFDLTDPAGGPFP
                     ADRFTVPDESQLTGLRVNLPEPDCSVRPSDCDDIDVLNTLDGFNLQPRLSIPFTGPID
                     VATVSSEAMFLFKLSCTICPGGSVVGINQVVWDPATNTLYAESDQFLDQDARYLFVVT
                     NDIRDPSGERIDAEQFRDVLHSGQTNDPAETAYREALLAALDRLKDAGVPPGHVAAAS
                     IFTTQSATAVLEKIRDQLDQATPGPADFVLGSNGERTVFPLADVASIAFNRQITTAPP
                     FGTSPVAVNVLKAAGAVGAIAFGRFRSPDYETPERVIPAVGTRGTPAVQAVNEVYFNL
                     VLPAGTPPAGGWPVAIVGHGAGVGNKNDAGEMRNAAALAQRGMAAIAFNAIGFGGGPL
                     GTLVVTKTDGSTVTLSAGGRGGDDDGNTVIGAFEGHGTVPGTPQALVWVRDGISQTVI
                     DAMQLVRVIGAGMDVDADSNADLDPSRIYYLG"
BASE COUNT          376 a          803 c          754 g          318 t
ORIGIN      
        1 cccgatgacg tgctgacggg cccgagccgc ctccgcgaac tcgggcgcga accgctggac
       61 ccaccggtag atgctcacgt ggtcgacctc gatgccgcgc tcggcgagga gctcctcgac
      121 gtcccggtag gacaggccga agtgcaggta ccagcgcacc gccagggtga tgacttcggg
      181 agggaagcgg aacccggcga aggctgacct cggggccggg cagaggcaag ggtgttggag
      241 tgatctcatc gcgccagtct cggtgacgga ggcccggacg accaatgcaa cagtgcccgt
      301 cgttttggta cccctcaggc tccaagaggc accgcctcac ggtgccccaa acgaccggct
      361 gaaggccatc acgaaaagca tcgccgccca cccgagggac ccgagatgcc aggagctgct
      421 agtggaggca gtaccgcatc ttcgccgcgg gatcgtcgcc ctgcgccagt acgccgagcg
      481 gcacggggtc cctgacgagc tgatccgccg ggcggaggcg cgccacagaa gccggcacgc
      541 ctgacttccg cgcgatacgc gctggctcac cttgacaagg tgggttacag aggacaaggc
      601 taccgatacg ggactttgga ggcagagcgg cttgggcggc ggccgaaggg cattgacggc
      661 caggcccgat cgagacccgg gcagccccga acccaggtcg cccttgggcg gcaagacacc
      721 acgacggtgc ttcgatgtcc ctcctgcgcc gcatggtgct ggttacgccg acgcccccac
      781 gcaggaacca ggaggagggg acccgatgaa agcccggatc attctcatct tgtcggccgc
      841 tatatgcctt gcgttcgccg cggctgcgcc cgcggcgctg gcggacgggg tcgagccccg
      901 cttcgacctc accgatccgg ccggcgggcc cttcccagcc gaccggttta ccgtccccga
      961 cgagagccag ctgaccggcc tgcgggtgaa cctgcccgag cccgactgct ctgtccgccc
     1021 ctcggactgc gacgacatcg atgtgctgaa caccctcgat ggcttcaacc tccagccccg
     1081 gctctccatc cccttcaccg gcccgatcga tgtggccacc gtgtcgagcg aggccatgtt
     1141 cctgttcaag ctgagctgca cgatctgccc gggcgggagc gtcgtcggca tcaaccaggt
     1201 cgtgtgggat ccggcgacga acaccctgta cgcggagtcc gaccagttcc tcgaccagga
     1261 cgcccgctac ctgttcgtgg tcacgaacga catccgggac ccgagcgggg agcggatcga
     1321 cgccgagcag ttccgcgacg tcctccactc cgggcagacg aatgatcccg ccgagaccgc
     1381 ataccgagaa gcgctccttg ccgccctgga tcgcctgaag gacgccgggg ttccccccgg
     1441 ccacgtcgcc gccgccagca tcttcaccac ccagagcgcc acagcggtgt tggagaagat
     1501 ccgcgaccag ctcgaccagg cgaccccggg gccggccgac ttcgtgctcg gctcaaacgg
     1561 cgagcgcacc gtgttcccgc tcgccgacgt tgccagcatc gccttcaacc gccagatcac
     1621 gacggcgccg ccgttcggca ccagccccgt cgcggtcaac gtgttgaagg cagccggggc
     1681 ggtcggcgcg atcgccttcg gcaggttccg ctcgcccgac tacgagacgc ccgagcgcgt
     1741 gatcccggcg gtcggcacgc gcggcacgcc cgccgtccaa gccgtgaacg aggtgtactt
     1801 caacctcgtg ctgccggcag gcactccgcc cgcggggggc tggccggtgg cgatcgtcgg
     1861 ccacggcgcc ggcgtcggaa acaaaaacga cgccggtgaa atgcggaacg ccgcggccct
     1921 tgcccagcgc gggatggccg cgatcgcctt caacgcgatc ggcttcggcg gcggcccgct
     1981 ggggacgctc gtggtgacca agacggatgg cagcacggtg acgttgtcgg ccggcggtcg
     2041 cggcggcgac gacgacggta acaccgtgat cggtgccttc gagggacacg gcaccgttcc
     2101 ggggacgccg caggcgctcg tctgggttcg cgacggcatc agccagacag tcatcgacgc
     2161 gatgcagctc gtgcgcgtga tcggggccgg gatggacgtc gacgccgact ccaacgctga
     2221 tctggacccg tcccgcatct actacctcgg c
//
LOCUS       DASPWO010000547         6867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1096948, whole genome shotgun sequence.
ACCESSION   DASPWO010000547 DASPWO010000000
VERSION     DASPWO010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096948"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VEO00_12585"
     CDS             <1..389
                     /locus_tag="VEO00_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYU58867.1"
                     /translation="DANPANDQATASTQVVANAAGCTIVGTAGDDIISGTGGADVICG
                     GGGNDKLTGGGGNDQLRGEAGNDTLNGGFGNDVLNGGLGFDSHYGDSGADALQAKDGV
                     GNEVVNGGIGTDSCQSDPTDRRSSCP"
     gene            complement(415..624)
                     /locus_tag="VEO00_12590"
     CDS             complement(415..624)
                     /locus_tag="VEO00_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58868.1"
                     /translation="MQLFTSGQRGPGTTYQLAFIGAGRFAYTCSNHPSRMDHGRGTYR
                     FRARMRKPAAGRASKYSPPAAITVS"
     gene            697..933
                     /locus_tag="VEO00_12595"
     CDS             697..933
                     /locus_tag="VEO00_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58869.1"
                     /translation="MTVDGVKEKSVTDTCVAPPPPGRWACPESGSIISEEASTASNWP
                     AGTDFSSSRTSFDHPVSEAPPKNQFDPLSVRMRP"
     gene            1807..2850
                     /locus_tag="VEO00_12600"
     CDS             1807..2850
                     /locus_tag="VEO00_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYU58870.1"
                     /translation="MEPEREALFEVARRTFDTPEFRGIEFIESEAKTIINHVPGTWLP
                     FNWTINPYRGCTHACSYCFARPTHTYLDMNAGRDFETKIVVKVNAPQILRAQLGSKRW
                     KGEHIAMGTATDPFQRAEGRYRLMRGIIEALADYRNPFSILTKGTLILRDLDLLREAA
                     KITDVSTAFSIGTLDEDAWRRSEPGTPHPRARIEAVAALNDAGIPCGVLMAPILPGIT
                     DDPGRLRDVVRAAIDAGATHVSPILLHLRPGVREEFIPWLAEHYPDLVERYERTYAAR
                     GGYAPKAVQKEIGDVVRSLVREAGGVQVERGMANRLRRDEPAASPALPPVPPGQQLSL
                     LDAMPTARRRRAS"
     gene            2917..3417
                     /locus_tag="VEO00_12605"
     CDS             2917..3417
                     /locus_tag="VEO00_12605"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HYU58871.1"
                     /translation="MTRVREHLVGRGVEFEEISHSTAYTGIDEARAMGIEADDVAKTV
                     VVRAGDGFVLLAVPASRRLAMRRVQSAVGDNHARLATEDEIEGAFPGFQLGALPPLGS
                     LLDRPLYVDPELLRHDTIVFAAGTQTDSVRVRTADLFGGEPFSEAPLTRHPEGEDEDE
                     DEAPDG"
     gene            3430..3503
                     /locus_tag="VEO00_12610"
     tRNA            3430..3503
                     /locus_tag="VEO00_12610"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3464..3466,aa:Ile,seq:cat)
     gene            3701..4812
                     /locus_tag="VEO00_12615"
                     /pseudo
     CDS             3701..4812
                     /locus_tag="VEO00_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875750.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(5330..5887)
                     /locus_tag="VEO00_12620"
     CDS             complement(5330..5887)
                     /locus_tag="VEO00_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58872.1"
                     /translation="MRRSFVVLAAMLAALTLLATQALAADITGTPGNDYLYGTNGNDN
                     INGLAGHDWIWGNGGGDIIIGSYGFDKLIGNDGPNHLYGRSGKDTLWGEEQGCGFFTS
                     GGLHATGGAIPCPSPDLDQFYGGTNDDVIHADDRFVQPGDPTLGEFIDCGGGFDLAFV
                     DFSDFARAQSGQSNLFTTCEAYLVA"
     gene            complement(6089..6568)
                     /locus_tag="VEO00_12625"
     CDS             complement(6089..6568)
                     /locus_tag="VEO00_12625"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYU58873.1"
                     /translation="MSLDRSYVQHNDRERERLRALVERLSDDELGSAVNEHWTVAGVL
                     GHIGFWDGRVLALADKLEGGVPFSPSDEEPEDVDWINDAARPLIHAIVPREAARLALR
                     LAEETDQRVASLPPERMWPADANSPLNPLRATHRGEHLDEIEAALRARGAPEATERT"
     gene            6605..6687
                     /locus_tag="VEO00_12630"
     tRNA            6605..6687
                     /locus_tag="VEO00_12630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6638..6640,aa:Leu,seq:tag)
BASE COUNT         1029 a         2372 c         2450 g         1016 t
ORIGIN      
        1 cggacgcgaa cccggcgaac gaccaggcca ccgcgagcac ccaggtggtg gcgaacgccg
       61 ccgggtgcac gatcgtcggg acggcgggcg acgacatcat ctccgggacc gggggggccg
      121 acgtcatctg tggcggcggc gggaacgaca agctgaccgg cggcggcggg aacgaccagc
      181 tgcgcggcga ggccgggaac gacacgctga acggggggtt cggcaacgac gtcctgaacg
      241 gagggctcgg attcgacagc cactacggcg attccggtgc cgacgccctg caggccaagg
      301 acggcgtggg caacgaggtc gtgaacggcg ggatcgggac cgactcgtgc cagagcgacc
      361 cgaccgaccg tcgctcgtcc tgcccctgac cgctcgacgg cggacggccc gtcgtcagct
      421 gaccgtgatg gccgccggag gcgagtactt cgacgcgcgg ccggcggcgg gcttgcgcat
      481 ccgggcacgg aaccggtagg tcccccgacc atggtccatc cgtgacgggt gattcgagca
      541 ggtgtaggcg aaccgcccgg cgccgatgaa cgccaactga tacgtggtgc ccgggccgcg
      601 ctgccccgag gtgaacagct gcatcccgcc cgagtccgtg gccgtgtgag tgctcggccc
      661 ggcgaagctc cagcggacgc tcccgcctgc gaggcggtga cggtcgacgg tgtgaaggag
      721 aagtcggtca ccgacacctg tgtcgcgccg ccgccccccg gccggtgggc ctgcccggag
      781 tccgggtcga tcatcagcga ggaggcgtcc acggcctcga actggcctgc cggcacggac
      841 ttcagctctt cgaggacgtc gttcgaccac ccggtctccg aggctccgcc gaagaaccag
      901 ttcgacccgt tgtcggtcag gatgaggccg taggtcttca tcgcctgcag gaccacccgc
      961 gcctccggcg agaacccgtt cagcgagaag cgcgccctca gccggaaccg cgcgcccatc
     1021 ggcggcaggt tggcgtggcg ccgcgcgccg gcctggtgcc gggccggcca gacgtaggtg
     1081 gtgtcggtct cccgcgcggt gaaccggatg gcgtggtcga tgaccccggc cacgacttcg
     1141 tcgcggcgga tcagcccggc gaggatcggg agcccggccg cgtccgccga tgtccacccg
     1201 gccgggcgaa gcgcgttcga gcgcaggtcg aacactgcgc ccgatcctgc ggtcgagccg
     1261 ccgctcggcc ggtacgtcgc gtcgtagagc tcgaagagcg cgcaggcgtc cttgtcgacc
     1321 atcagcgcgt gccggtccga gccgccctcg atcgtgatgt cggggccgaa ggggtagagc
     1381 acccggtcgc tctcgtccgg gtacgagaag tcgaccggca cgtcctggtg cgacgcgccg
     1441 atcacgttga acgggatgcc gtacggctgc ccgtcggagg gcccgaagtc cgggtgcagg
     1501 ttggtggtcg acgcgtccat cgaggccatc caggccgcgc tcttggcatg caccgggagc
     1561 cgcgagatgt ccgagtgcca gacgttgtcg tcggggaaga cggagcacgt gctgccgggc
     1621 ggcgggatgg ccgtggccgg gctgcaggcg agcaggctgc ccagaagggc gacggcaacc
     1681 agggccggaa ggggccgcat ccgccgagcc acgctacagg tatcgacccc cgtgcggcgg
     1741 ccctgaaccc ggaacgccga gcacggccgg gttgcgggaa tcgaacgtat gttctatcgt
     1801 cccgccatgg aaccggagcg cgaggccctg ttcgaggtgg cccggcggac cttcgacacc
     1861 ccggaattcc gcgggatcga gttcatcgag tccgaggcca agaccatcat caaccacgtc
     1921 ccgggcacct ggctgccgtt caactggacg atcaacccgt atcgaggatg cacacatgcc
     1981 tgcagttact gtttcgctcg gccgacgcac acgtacctcg acatgaacgc agggcgggac
     2041 ttcgagacga agatcgtggt gaaggtgaac gcgccgcaga tattgcgggc gcagctcggg
     2101 tcgaagcggt ggaagggcga gcacatcgcg atggggacgg cgaccgatcc ctttcagcgg
     2161 gcggaggggc gctaccggtt gatgcgcggg atcatcgagg cgctcgccga ctaccgcaat
     2221 cccttctcga tcctgacgaa gggaacgctc atcctgcgcg acctggacct gctgcgcgag
     2281 gccgcgaaga tcaccgacgt ctcgaccgcg ttctcgatcg ggacgctcga tgaggacgcc
     2341 tggcgccgca gcgagccggg cacgccccac ccgcgagccc gaatcgaggc cgtggcggcg
     2401 ctgaacgacg cggggatccc gtgcggcgtg ctgatggccc cgatcctgcc gggcatcacc
     2461 gacgatcccg gcaggctgcg cgatgtggtg cgcgccgcga tcgacgccgg cgccacccac
     2521 gtctcgccga tcctgctgca cctccgcccc ggcgtgcgcg aggagttcat cccctggctg
     2581 gccgagcact atccggacct cgtcgaacgc tacgagagga cgtacgcggc ccgcggcggc
     2641 tacgcgccca aggccgtgca gaaggagatc ggcgacgtgg tccgctccct ggtgcgcgag
     2701 gccggaggcg tgcaggtaga gcgcggcatg gcgaaccgcc ttcgccgcga cgagccggct
     2761 gcatcaccag cgctcccgcc ggtgccgccc gggcagcagc tctcgctgct cgacgccatg
     2821 cccaccgccc ggcgacgccg cgcgtcctga ggtccgcggt gcggcccggc cgcgggtcgg
     2881 gttgaatggg accccagcgc ggaggggagg cgggccatga cgcgggtcag agaacacctg
     2941 gtggggcgag gggtcgagtt cgaggagatc tcgcactcca cggcctacac gggcatcgac
     3001 gaggcgcggg cgatggggat cgaggcggac gacgtggcca agaccgtcgt ggtcagggcc
     3061 ggtgacggct tcgtcctgct ggccgtgccc gcgtcccggc gcctcgccat gaggcgggtc
     3121 cagtcggcgg tcggcgacaa ccacgcgcgg ctggcgaccg aggacgagat cgagggcgcc
     3181 ttccccgggt tccagctcgg cgcgttgccg ccgctcggct ccctgctcga ccgtcccctg
     3241 tatgtcgatc ccgagctgct gcggcacgac acgatcgtgt tcgccgcggg gacgcagacc
     3301 gactcggtgc gggtccggac ggccgacctg ttcgggggcg agccgttctc cgaggcgcca
     3361 ctgacccgcc acccggaagg cgaggacgag gacgaagacg aggcccccga cggctagggt
     3421 gtgacccgag gggcggtagc tcagtcggtc agagcagcgg actcataatc cgccggtcgc
     3481 tggttcgagt ccagcccgcc ccacccctgt ttgtcgtgct tcgggtgatg ctggacgttc
     3541 cggcgtcacc tgatccttga cacggctccg ggtgatcctt gacacgtgtt tcggctgatc
     3601 cttgacggtg gctccggctg atgcttgaca ccgcgccgcc ctgacgtctg cttgcgcaaa
     3661 cgacaggagg aggtgcgcgg tgctcgtgga gctgagcgtc atggagcagc ggtaccaggc
     3721 ggtgctggcg gtggtgcagg acggctggcg ggtggtcgag gtggcccgcc gcctcggcgt
     3781 gtcccgccag agcgtccacg cctggatcgc ccggtacgag gccggcggcc tggccgccct
     3841 ggccgaccgg tcgcaccggc ctgccgcgtg tccccaccag atcgccccgg aggtcgaggc
     3901 ccgcatctgc gagctccgcc gtgagcaccc cgggtggggg ccgcggcgga tcgagcacca
     3961 gctcgcgagg ctcggcgtcg agcccgttcc atcccgctcg agcatctacc gctgtctcaa
     4021 gcgacacggc ctgatcgaac tccgccgccg gcgccgccgc cgcgacgagt tccgccggtg
     4081 ggagcgcgac cgccccatgc agctgtggca gatggacgtc atcggcgggg tcctcctcga
     4141 cgacgacacc agctgaaggt ggtgaccggc gtcgatgacc acagccggtt ctgcgtcgcg
     4201 gccggcctgg tcagacgggc gacggccagg gcggtgtgcg agctgttcgc cgcgtccctc
     4261 cgccgctacg ggatccccga cgaggtcctg accgacaacg gcaaggtgtt caccgggcgg
     4321 ttcggtcccc acccggtgga ggtgctgttc gaccggatct gccgggagaa cggcatctcc
     4381 caccgcctga ccggggttcg ctcgcccacg accaccggca aggtcgagcg gttccaccag
     4441 agcctgcgcc gggagttcct ggccgagcgg accttcccct cgctcccggc ggctcaggcc
     4501 gccctcgacg cctgggtggg tgagtacaac accgtgcgtc cccaccaggc gctcgagatg
     4561 gccaccccgg cggagcgctt ccgtctggag cccctcgccg ccgaggccgc gtcggtcccg
     4621 gtcgacgacc aggcggacca cgccgggcag tgggtcctgc gccgggtcgc ctcgaacggc
     4681 gtggtgtcgg tcgacaacca gatgttctcg gtgggcaacg cctacaaggc ccagctcgtc
     4741 gacgtcttcg tcgacgacac ggtgatccag gtgtggagta agaaccacct catcaagagc
     4801 gtcgccagga cgaggaccgg gtcggtccgc aaggtccggg ccgacgccct ccacgtcaag
     4861 catcagccga ctacagttcg ttcaagcatc aggtggctct tgacagggcc cacctcatct
     4921 ccgcagatca atgggcacga acagttgccc gccagatcag tggcgggcaa cgttggggcg
     4981 aatctgggga gaaccgcgct cggaccggct ctgcaagcga gctagttctc cgtgccgcca
     5041 tggcgttgag gcaagccacc agcacgaacg aatccgggcg tccaaccctg gtcagctccc
     5101 ctcctcacgc cctcctgatc cgtcggagga gcggctgcgt gagcaggagg aagccggggt
     5161 ccggatgccg ccgatcagtc ggacggagct cgcgctcagg atcctcgtgg ccgccgcgat
     5221 gtgtggggct actggctgac gtcgcgactc ttggtcacca gcttcctcga atctcctcgt
     5281 tcaacggacg gaggccttgt ccggcctcgt ccgttacgtt ctcgctcgct caggcgacga
     5341 ggtacgcctc gcaagtcgta aatagattgc tctgtcccga ctgggcgcgg gcgaagtcgc
     5401 tgaagtccac gaaggcgagg tcgaacccgc cgccgcagtc gatgaactcg cccagggtcg
     5461 ggtcgcccgg ctgaacgaac ctgtcgtcgg cgtggatcac gtcgtcgttg gtgccgccgt
     5521 agaactggtc gaggtccggg gagggacacg ggatggcacc gccggtggcg tgcaggccgc
     5581 cactggtgaa gaacccgcag ccctgctcct cgccccacag cgtgtccttg ccggaacgcc
     5641 cgtagaggtg gttgggcccg tcgttcccga tcagcttgtc gaacccgtag gacccgatga
     5701 tgatgtcgcc gccgccgttc ccccagatcc agtcgtggcc ggccaggccg ttgatgttgt
     5761 cgttcccatt cgtgccgtag aggtagtcgt tgccgggggt gccggtgatg tcggcggcca
     5821 gggcctgggt cgccagcagg gtgagcgcgg ccagcatcgc ggccagcacg acgaaggatc
     5881 tgcgcatcga acgtcgcctc cttcggcggc cggtggccgc gatgccccaa ggtatacgcc
     5941 gccacacgcc cggcacaagg gtttggatcg cgatccggga acagtcccca gcgcagggca
     6001 gcgaggcccg tctcggtcct cgatcggcgg ggttctcgcg tgtcgggcac ggcatcaggc
     6061 gagcctggcc gaccttcctg ccgcgcgatc aggtgcgctc ggtcgcctcg ggcgctcccc
     6121 tggctcgcag cgccgcttcg atctcgtcga ggtgctctcc gcggtgcgtg gcccgcaacg
     6181 gattgagggg gctgttcgca tcagcgggcc acatccgctc gggcggaagc gaggccaccc
     6241 gctggtcggt ctcctcggcc agacgcaggg cgagccgggc cgcctctcgc ggcacgatgg
     6301 cgtggatcag cgggcgggcg gcgtcgttga tccagtccac gtcctccggc tcctcgtcgg
     6361 acggcgagaa cgggacgccg ccctccagct tgtcggccag ggccagtacg cgcccgtccc
     6421 agaagccgat gtgacccagc acgccggcca ccgtccagtg ttcgttcacc gcgctgccca
     6481 gctcgtcgtc gctcagccgt tcgaccaggg cgcgcaatcg ttcgcgctcg cggtcgttgt
     6541 gctgcacgta ggaacgatcg agcgacatcg ggtggcatga tagccagggt tgacgacatt
     6601 tctggcgaga gtggcggaac gggagacgcg ccggccttag gagccggtgc ccggaagggc
     6661 gtgggggttc gactcccccc tctcgcacct tctcccccat ccggccgaca gcgccggtgg
     6721 tcaggacagg tcaggcgaaa ccacccgttc gggctatcag cctgtccccc caccaagccg
     6781 acccttagag cgtgccgacc gccgtgcgcg tgctcgatgc gatggtgacc ggcgcctacc
     6841 tcgtcctggt cgccgtgctg gcccggg
//
LOCUS       DASPWO010000548         3678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_844874, whole genome shotgun sequence.
ACCESSION   DASPWO010000548 DASPWO010000000
VERSION     DASPWO010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844874"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VEO00_12635"
     CDS             complement(<1..967)
                     /locus_tag="VEO00_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYU58874.1"
                     /translation="MSESRVDPARIRNLCIVAHIDHGKSTLADRFLELTHAVAERDMR
                     AQYLDRMDLERERGITIKAQAVRLSFDGTVLNLIDTPGHVDFSYEVSRSLAACEGAIL
                     LVDAAQGIEAQTLANFHLARDAGLTIVPAVNKVDLPSADPDEVARELAELTGTAPPDV
                     LRISAKTGEGVEALLRAVVERIPPPAGDRSAPLRALIFDSAHDPYRGVIAYVRVVDGE
                     LRGRQRVRLMASGLHPETEEVGVIAPEPMPVPALAAGEVGYLVTGVKDVRKAQVGDTV
                     TDAASPAAEALPGYREPKPMVWSGLYPVEGGDYTLLRDALDKLRLS"
     gene            1024..1293
                     /gene="rpsT"
                     /locus_tag="VEO00_12640"
     CDS             1024..1293
                     /gene="rpsT"
                     /locus_tag="VEO00_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HYU58875.1"
                     /translation="MANIKSQIKRNRQNETRRLRNKSMRSALKTGARGVREAHASGDA
                     GEAQARLRETSRALDKAAAKGTLHKRRAADRKSRLARSLSAPGGE"
     gene            complement(1309..2271)
                     /gene="holA"
                     /locus_tag="VEO00_12645"
     CDS             complement(1309..2271)
                     /gene="holA"
                     /locus_tag="VEO00_12645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HYU58876.1"
                     /translation="MGKNVLLWGEDEFLLREAAAEAFGSLWEHVREVEGDAWTGGETG
                     DLATPSLFGEPRALLVSGCQDLSDEGAREVRAYLEHPAPDATLVLTGRGIRAAPPLAK
                     GLPAGIEVRRVEITRRDLPGWVKRRGERRGVRAEPAALAALIDTVGEDPGRLDQALEQ
                     LVNAFPDQPITSRHVEEQFRGLGDRKTWEVTDRAFGGDLAGAIRALAGMLAARDDPLM
                     ILGGIAARLRDLQRVAALPDGIHPRDLAREAGMRFDWQARRAREQARRYEPGDLAALH
                     RTVAEMDRAIKIGVPGEVILPLLLARISRAVPPREPYTAPRMGG"
     gene            complement(2285..>3678)
                     /locus_tag="VEO00_12650"
     CDS             complement(2285..>3678)
                     /locus_tag="VEO00_12650"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HYU58877.1"
                     /translation="LALGGAALGRGRAGATLLGTAVLVLLAWNPTLLRSVGFQLSVAA
                     TAGILAFAGPIQRRLPIPKGMAVAAGATVGAQAGVTPLLLFHFHVVPMATLPANLLAF
                     AAVGPALWLGLAAAGASLVWPAAGAVLAAPARLPLAWLTAVADRAASAPLPALTGGGL
                     PVLVAGTAAVAWLAWRLRTGRRLGPRLGVLLLAGPLLVWTLAARAGPPAELTVRFLDV
                     GQGDAALVQSPAGATVLIDGGQEPDDVAIQLAALGVRRLDVVVATHGHADHVGGLPAV
                     LTRFPVGVVLDPGCPADSPLYADFLRAVRVEGVPLRHPRSGDVLLVGDLRLQVLNPPG
                     CYVGTDSDPNNDSIVIRLSRERASVLFAAEVEEPAQENLLETSASNLRALVLKVPHHG
                     AGTSLAEFLVAAHAAVAVVSVGAGNDYGHPVPWVLATLRAAGMHVYRTDRSGEVVVRF
                     GAQGVLVESERAA"
BASE COUNT          512 a         1385 c         1368 g          413 t
ORIGIN      
        1 cggagaggcg caacttgtcg agcgcgtcgc gcaggagcgt gtagtcaccg ccctccaccg
       61 gatagagccc gctccagacc atcggcttcg gctcgcggta gccggggagc gcctccgcgg
      121 ccggactggc cgcgtcggtg accgtgtcgc cgacctgcgc cttgcgcacg tccttcacgc
      181 cggtgaccag gtagccgacc tccccggcgg cgagcgccgg caccggcatc ggctccgggg
      241 cgatcacgcc gacctcctcg gtctcggggt gcaggccact cgccatcagc cgcacccgct
      301 gccggccgcg cagctcgccg tccacgaccc ggacgtaggc gatcaccccg cggtaggggt
      361 cgtgggccga gtcgaagatc agcgcgcgca gcggggccga tcgatcgccg gccggcggcg
      421 ggatgcgctc gaccaccgcc cgaagcagcg cctcgacccc ctcgccggtc ttcgccgaga
      481 tgcgcagcac gtcggggggc gccgtcccgg tcagctcggc cagctcgcgg gccacctcgt
      541 ccggatccgc cgagggcagg tcgaccttgt tcactgccgg gacgatcgtg agaccggcgt
      601 cgcgggccag gtggaagttg gcgagcgtct gggcctcgat cccctgcgcc gcgtcgacca
      661 gcaggatggc gccctcgcat gccgcgagcg aacgggagac ctcatacgag aagtcgacgt
      721 gccccggcgt gtcgatcagg ttgagcacgg ttccatcgaa cgacaggcgc acggcctgtg
      781 ccttgatcgt gatcccccgc tcccgctcga ggtccatccg atcgaggtac tgggcacgca
      841 tgtcgcgctc ggcgaccgcg tgggtcagct ccaggaagcg gtcggccagc gtcgacttcc
      901 cgtggtcgat gtgggccacg atgcagaggt tgcggatgcg cgcggggtcg acgcgggact
      961 cgctcacggc aggccagcgt acggcatgga gtgactcatc ccccctgcta gactggccgg
     1021 ctcatggcga acatcaagtc ccagatcaag cggaaccggc agaacgagac gcgccgcctg
     1081 cgcaacaagt ccatgcgcag cgcgctgaag accggcgccc gcggggtccg cgaggcccac
     1141 gcctccggcg acgccgggga ggcccaggcc cgcctgcgcg agacgtcgcg cgctctggac
     1201 aaggccgccg cgaaggggac cctgcacaag cgccgcgccg ccgaccgcaa gtcgcgcctg
     1261 gcgcgctcgc tgagcgcccc cggcggcgag tagcccccgc cgctcccctc agccgcccat
     1321 ccggggcgcc gtgtagggct cccgcggcgg gacggcgcgc gaaatgcgcg ccagcagcag
     1381 cggcaggatc acctcgccgg gcaccccgat cttgatcgcg cggtccatct cggccaccgt
     1441 gcggtgcagc gcggcgaggt ctcccggctc gtagcggcgc gcctgttcgc gggcccggcg
     1501 cgcctgccag tcgaatcgca tccccgcctc ccgggccagg tcccgcgggt gaatcccgtc
     1561 cgggagcgcc gccacccgct gcaggtcgcg cagccgggcg gcgatcccgc cgaggatcat
     1621 cagcgggtcg tcgcgagcgg cgagcatccc cgcgagggcg cggatcgcgc cggccaggtc
     1681 gccgccgaag gcccggtcgg tcacctccca ggtcttccgg tcccccaggc cccggaactg
     1741 ctcctcgacg tgccgcgacg tgatcggctg gtcggggaag gcgttgacga gctgctcgag
     1801 cgcctggtcg agccgcccgg ggtcttcccc caccgtgtcg atcagtgcgg ccagggcggc
     1861 cggttcggcc cgaacgccgc gccgctcgcc gcgccgcttg acccatccgg ggaggtcgcg
     1921 gcgggtgatc tcgacccggc ggacctcgat cccggcaggg agccccttcg ccagcggtgg
     1981 cgcggcgcgg atgccgcggc cggtcagcac gagcgtggcg tcgggcgcgg gatgctcgag
     2041 gtaggctcgg acctcgcggg cgccctcatc ggacaggtcc tggcagcccg agacgagcag
     2101 cgcgcgcggc tcgccgaaca gcgacggcgt ggcgagatcg cccgtctcgc ccccggtcca
     2161 ggcgtcgccc tcgacctcgc gcacgtgctc ccagagggag ccgaacgcct ccgcggccgc
     2221 ctcgcgcagg aggaactcgt cctcgcccca gagcagcacg ttcttcccca acgattcact
     2281 cccgtcaggc ggcgcgctcg gattctacga gcaccccctg cgcgccgaac cggacgacca
     2341 cctcgccgct gcggtcggtg cggtagacgt gcatccccgc cgcgcgcaac gtcgccagca
     2401 cccacgggac cgggtgcccg tagtcgttcc cggcgccgac gctgacgacg gcgaccgcgg
     2461 cgtgcgcggc gaccaggaac tcggccagcg aggtcccggc cccgtggtgc ggcaccttca
     2521 ggacgagggc gcgcaggttc gaggctgagg tctccagcag gttctcctgc gccggctcct
     2581 ccacctcggc ggcgaacagg acggaggcgc gctcgcggga caggcggatg acgatcgagt
     2641 cgttgttcgg gtcggagtcg gttccgacgt agcagccggg cgggttgagc acctgcaacc
     2701 gcaggtcccc gaccagaagc acgtctccgg atcgagggtg gcgcaggggg acgccctcga
     2761 cgcgcacggc ccgcaggaag tcggcataca gcggcgaatc cgcggggcag ccggggtcga
     2821 ggaccacccc gaccgggaag cgcgtcagca ccgccgggag cccgccgacg tggtcggcgt
     2881 ggccgtgggt ggcgaccacg acgtcgaggc gccgcacgcc gagggccgcc agctggatgg
     2941 cgacgtcgtc gggttcctgg cccccgtcga tcagcacggt ggcgcctgcc ggcgactgca
     3001 ccagcgcggc gtcgccctgg ccgacgtcga ggaaccgcac ggtcagctcc gcgggcgggc
     3061 cggcccgggc ggccagcgtc cagacgagca gcgggccggc gagcagcagg acgccgaggc
     3121 gcggccccag gcgccgcccg gtcctgagac gccaggccag ccaggcgacc gcggctgtcc
     3181 cggccaccag gacgggcagg ccgcctccgg tcagcgccgg gagcggcgcg gacgcggcgc
     3241 gatcggcaac ggcggtcagc caggcgagcg ggagacgcgc cggcgccgcc aggacggccc
     3301 cggccgccgg ccagacgagg ctcgcgccgg cggcggccag tccgagccag agcgccgggc
     3361 cgaccgcggc gaaggccaac aggttcgccg gcagcgtggc catcgggacg acgtggaagt
     3421 ggaacaagag cagcggggtc acgccggcct gggcgccgac ggtggcgccg gcggccaccg
     3481 ccatcccctt cgggatcggc aggcgacgct ggatggggcc ggcgaaggcc aggatgccgg
     3541 ccgtggccgc caccgacagt tggaagccga ccgagcgcaa cagcgtcggg ttccaggcca
     3601 gcaagacgag gaccgcggtg ccgagcagcg tggccccggc gcgtccacgc ccgagcgccg
     3661 cgccgcccag ggcgaggc
//
LOCUS       DASPWO010000549         5671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1097831, whole genome shotgun sequence.
ACCESSION   DASPWO010000549 DASPWO010000000
VERSION     DASPWO010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097831"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VEO00_12655"
     CDS             <1..336
                     /locus_tag="VEO00_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HYU58878.1"
                     /translation="AGQGQGLSEYFLGQESGVESVTLITTEMPLHTHTASAHAFDPGD
                     VFAPGPTVVPATADGGFAYNQTANANLTQFSPQALSIAGGSLPHNNMMPYLTLNFCIA
                     LQGVFPPRG"
     gene            355..861
                     /locus_tag="VEO00_12660"
     CDS             355..861
                     /locus_tag="VEO00_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU58879.1"
                     /translation="MAIQTSTVTLRPARPEDRDFLLAVYASTRAEEMAIVPWTDEQKD
                     AFVRFQFEAQDRHYREHFARASFDVVLVGGDPAGRLYVDRGPAEIRVVDIAILPEFRG
                     AGVGGMLLGELLNEAERTGRKVSIHVERFNRAIGLYRRMGFTPVADAGGDAYVLMEWV
                     RPSERSTR"
     gene            858..2126
                     /locus_tag="VEO00_12665"
     CDS             858..2126
                     /locus_tag="VEO00_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYU58880.1"
                     /translation="MKEERLTISRRRFLQAAGAGAGAAAMGPVLLADRAFASTSLSLR
                     VGVMAPSGGEYTSAGANLLEGIRLRLAQVHKQVGSSVTAVEVPLGFGGADGAAEQLLS
                     GGVDVVIAGITAPVAGQVASTFERQRVPLIVANLGAHAVRQSARSPYVLHHGLHYWQS
                     SFSFGSWAASHLGSRAFVAAPLADTGYDTVYAFRRGLESAGGQVVGTFVTETSDDDST
                     LRSLFAAASAASPDFVYALFSGSRAARFVRGYASSALGGRVPLTGGGLMVDDYLLSQI
                     GSAAFGVRNALPWVRTASDAAAVAFRKAFRRRAGREPDAFALIGYEAAMLAVTGAQAA
                     RQQGKPSKELISALAGADVAAPRGTLTVDGGSNTVRSGVQIRTVRSVNGSLANVSLER
                     VAALAAFPGELSSLDTAMSSGYLNEYLHAQ"
     gene            2123..2431
                     /locus_tag="VEO00_12670"
     CDS             2123..2431
                     /locus_tag="VEO00_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58881.1"
                     /translation="MTEFPDLAAFEQCRGDEFRLRPDEGESVPLVLDQVTALGDGRPR
                     PGLARQEPFSLLFRGPPGFAAPQRIWPLEHDRLGELRIFLVPVGQDQEGRMRFEAIFN
                     "
     gene            complement(2499..5093)
                     /gene="clpB"
                     /locus_tag="VEO00_12675"
     CDS             complement(2499..5093)
                     /gene="clpB"
                     /locus_tag="VEO00_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HYU58882.1"
                     /translation="MDLNKLTMKSRAALEAASAQAGARNHQNIEPEHVLFALLSDPEG
                     VPYPLLHAMGLSPRALRDRVDEALDRMPKVYGPSGSEAQLSPATRRVLERAFAEAEQL
                     TDEYVSTEHLLLAMIDGPAGVARLVADAGLTRDGVLQALAKVRGRQRVTDPNPEEKYQ
                     ALERYGRDLTELARSGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIVAGDVPDGLKGKRVVALDIGALVAGSKYRGEFEERFKAVLKEIAESGGEIIT
                     FIDELHTVVGAGAAEGSVDAGNMLKPMLARGELRAIGATTLDEYRKHVEKDPALERRF
                     QPVFVGEPSVEDTVAILRGLKERYEVHHGVRIQDAALVAAALLSNRYVTGRFLPDKAI
                     DLIDEAASRLRIEIDSMPVEIDEVERRIKQLEIERAALRKEKDAASKGRLEKLEAELA
                     DLRERSSGMKAHWEREKEHIDRIRQLNSEIERARSQAELAERDGDLERAAELRYGTII
                     ELQRQMEEAQRALADLQADTKMLKEEVDDEDVAEVVSKWTGIPVSRLLEGEIQKLVRM
                     EDAIHERMVDQDEAVAAVANAIRRSRAGLSDPNRPIGSFVFLGPTGVGKTELARSLAD
                     FLFDDERAVVRIDMSEYQERHAVSRLIGAPPGYVGYDEGGQLTEAIRRRPYSVILLDE
                     IEKAHSDAFNVLLQLLDEGRLTDGQGRTVDFRNTVVIMTSNLGSYVFNDVTSGEEERR
                     EKVMAEVRATFRPEFLNRVDEIIVFSPLGRDDIAKIVDIQLRILQERLAARRLTVELT
                     EGARDYLADTGFDPAYGARPLKRLIQREIQDALAMQLLSGDIRDGDTIVIDRGDAGLE
                     FRTAAPSR"
     gene            complement(5084..5638)
                     /locus_tag="VEO00_12680"
     CDS             complement(5084..5638)
                     /locus_tag="VEO00_12680"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HYU58883.1"
                     /translation="MTRVVLRRAGVTLRPYRKGELEDMEAAMRTEEAREWLPLGPPPR
                     QELRRRVDAGGLLSDGRIDLVIEVDGRMVGEIDGRHPRWALPPGVWELGITVFSRADR
                     GRGHGREALRLMCDRLFIEESAHRVSLTTDVENRAMRAVAERVGFRMEGVLRSFMPTG
                     QGRRDYAMYAMTKDDWENERTTWT"
BASE COUNT          747 a         2034 c         1992 g          898 t
ORIGIN      
        1 gccgggcagg gccagggcct gtccgagtac ttcctggggc aggagtccgg ggtcgagtcc
       61 gtgaccctga tcaccacgga gatgcccctg cacacccaca cggcatccgc ccatgccttc
      121 gaccccggcg acgtcttcgc gcccggcccc acggtcgtgc ccgcgaccgc ggacgggggg
      181 ttcgcgtata accagaccgc gaacgcgaac ctgacgcagt tctcaccgca ggcgctgtcg
      241 atcgcggggg gcagcctgcc ccacaacaac atgatgccgt acctcacgct gaacttctgc
      301 atcgccctgc agggcgtctt cccgccgcgg ggctgagggg gaacgaggga aggagtggcc
      361 atccagacat ccaccgtcac cctgaggccg gcgcgaccgg aggaccgcga tttcctgctc
      421 gcggtgtacg cctccacgcg ggccgaggag atggcgatcg tcccctggac cgacgagcag
      481 aaggatgcgt tcgtccggtt ccagttcgag gcacaggacc ggcactaccg cgagcacttc
      541 gcccgggcgt cgttcgacgt cgtcctcgtc ggcggcgatc cggcggggcg cctgtacgtg
      601 gaccgcgggc cggcggagat ccgagtggtc gacatcgcaa tcctgccgga gttccgcggg
      661 gccggagtgg gcggcatgct gctcggcgag ctgctgaacg aggccgaacg gacggggcgc
      721 aaggtgtcga tccacgtcga gcggttcaac cgggccatcg gcctgtaccg gcggatgggc
      781 ttcacgccgg tcgcggatgc cggtggcgac gcgtacgtgt tgatggaatg ggttcgtccc
      841 agtgagagga gcacccggtg aaggaggaac ggttgacgat cagccggcgc cgcttcctgc
      901 aggcggccgg ggcgggggct ggtgcggcgg cgatggggcc ggtcctgctg gccgaccggg
      961 cgttcgcatc gacctccctg agcctccggg tgggagtcat ggcgccatcc ggcggcgagt
     1021 acacgagcgc cggggcgaac ctgctggagg ggatccgtct ccggctggcc caggtccata
     1081 agcaggtggg gtcatcggtg acggccgtcg aggtaccgct ggggttcggc ggcgcggacg
     1141 gggcggccga gcagctgctc tcgggcggcg tcgacgtggt catcgcaggc attaccgccc
     1201 cggtagcggg gcaggtggcc tcgacgttcg agcggcaacg tgttcctctg atcgtggcga
     1261 acctcggcgc gcacgccgtc cggcagtcgg cgcggagccc ctacgtcctg caccacgggc
     1321 tgcactactg gcagtcgagc ttctcgttcg ggtcctgggc cgcgagccac ctggggtcgc
     1381 gcgcgttcgt ggccgcgcca ctggccgaca ccggctacga caccgtgtac gcgttccggc
     1441 gcggcttgga gtcggccggt gggcaggtcg tggggacgtt cgtcaccgag acgtccgacg
     1501 acgattccac gctccgcagc ctcttcgcgg cggcgtcggc ggcctcgccg gacttcgtgt
     1561 acgcgctgtt ctccgggagc cgggcggccc ggttcgtccg ggggtacgcc tcgtcggcgc
     1621 tcggtggccg ggtgccgctc accggcggcg ggctcatggt ggacgactac ctgctgtccc
     1681 agatcggctc ggcggcgttc ggtgtccgca acgcgctgcc gtgggtccga accgcgtccg
     1741 acgcggcggc cgtcgcgttc cggaaggcat tcaggcggcg ggccgggcgg gagcccgacg
     1801 cgttcgcgct catcgggtac gaggcggcga tgctggccgt caccggggcg caagcggccc
     1861 ggcagcaggg gaagccgtcg aaggagctga tctccgcact cgccggcgcg gacgtcgcgg
     1921 ccccacgggg gacgctcact gtggacggcg gatcgaacac ggtccggagc ggcgtgcaga
     1981 tccggacagt ccggtcggtg aacgggagcc tggcgaacgt ctcgctcgag cgggtggcgg
     2041 cgctggcggc gttccccggc gagctctcgt ctctcgacac cgcgatgtcg tccggatacc
     2101 tgaacgagta cctgcacgcg cagtgaccga gttccccgat ctcgccgcat tcgagcagtg
     2161 ccggggcgac gagttccgtc tgcgcccgga cgagggcgag agcgtgccgc tcgtgctgga
     2221 tcaggtgacc gcgctgggcg acggccggcc ccggccgggc ttggcccgcc aggagccgtt
     2281 ctcccttctc ttccggggtc cgccgggctt cgccgcgccc cagcggatct ggcccctcga
     2341 gcacgaccgg ctcggcgagc tccgcatctt cctggtgccg gtcggccagg accaggaagg
     2401 gcggatgcga ttcgaagcga tcttcaactg acgttcaccg agatccgctg ggagaacccg
     2461 ctggccgctc ggggctgacg acccgcggcg acgagccgct accgggaggg tgcggccgtc
     2521 cggaactcca atccggcgtc gccgcgatcg atcacgatcg tgtcgccgtc gcggatgtcg
     2581 cccgaaagga gctgcatggc cagcgcgtcc tggatctccc gctggatcag gcgcttcagc
     2641 ggccgggcgc cgtacgcggg gtcgaagccc gtgtcggcca ggtagtcccg ggccccctcg
     2701 gtgagctcca ccgtcagccg ccgcgcggcc aggcgctcct gcaggatccg gagctggatg
     2761 tccacgatct tcgcgatgtc gtcgcggccg aggggcgaga acacgatgat ctcgtccacc
     2821 cggttcagga actccggacg gaacgtggcc cggacctcgg ccatgacctt ctcccggcgc
     2881 tcctcttccc ccgaggtcac gtcgttgaac acgtacgagc ccaggttcga ggtcatgatc
     2941 accacggtgt tccggaagtc cacggttcgg ccctggccgt cggtgagccg gccctcgtcg
     3001 agcagctgga gcaggacgtt gaacgcgtcc gagtgggcct tctcgatctc gtcgagcagg
     3061 atcaccgagt acggccgccg ccggatcgcc tcggtgagct gtccgccctc gtcgtagccg
     3121 acgtagccgg gcggcgcccc gatcagccgg ctgaccgcgt ggcgctcctg gtactcgctc
     3181 atgtcgatgc ggaccacggc ccgctcgtcg tcgaacagga agtcggccag cgagcgggcc
     3241 agctcggtct tccccacacc ggtggggccc aggaacacga acgagccgat cgggcggttg
     3301 gggtcggaca gcccggcccg ggacctgcgg atggcgttcg ccacggcggc gacggcctcg
     3361 tcctggtcca ccatccgctc gtggatggcg tcctccatgc ggaccagctt ctggatctcg
     3421 ccctccagga ggcgcgagac ggggatgccg gtccacttcg agacgacctc ggccacgtcc
     3481 tcgtcgtcga cctcctcctt cagcatcttg gtgtcggcct ggaggtcggc cagggcccgc
     3541 tgggcctcct ccatctgccg ctggagctcg atgatcgtgc cgtagcggag ctcggcggcc
     3601 cgctccaggt cgccgtcgcg ttcggccagc tcggcctgcg aacgggcccg ctcgatctcg
     3661 gagttcagtt ggcggatccg gtcgatgtgc tccttctccc gttcccaatg cgccttcatg
     3721 ccggagctcc gctcgcggag gtcggccagc tcggcctcga gcttctccag ccgccccttc
     3781 gacgccgcgt ccttctcctt gcggagtgcg gcgcgttcga tctcgagctg cttgatccgc
     3841 cgttccacct cgtcgatctc cacgggcatc gagtcgatct cgatccgcag ccgcgaggcc
     3901 gcctcgtcga tgaggtcgat ggccttgtcc gggaggaacc ggccggtcac gtagcggttc
     3961 gacagcaggg ccgccgcgac caacgcggcg tcctggatcc gcacgccgtg gtggacctcg
     4021 tagcgctcct tgagcccccg aaggatcgcc acggtgtcct ccaccgacgg ctcgcccacg
     4081 aacaccggct ggaaccgccg ctccagggcg gggtccttct ccacgtgctt gcggtactcg
     4141 tcgagcgtgg tggcgccgat ggcccggagc tcgccgcgag ccagcatggg cttcagcatg
     4201 ttccccgcgt ccaccgagcc ctcggcggcc cccgcgccga ccacggtgtg gagctcgtcg
     4261 atgaacgtga tgatctcgcc gccggactcc gcgatctcct tcagcacggc cttgaaccgc
     4321 tcctcgaact caccccggta cttggatccg gccaccaggg cgccgatgtc gagcgccacc
     4381 acccgcttgc ccttcaggcc gtccgggacg tcgcccgcca cgatgcgctg ggccaggccc
     4441 tccacgatcg cggtcttgcc cacgccgggc tcaccgatga ggaccgggtt gttcttcgtc
     4501 cgacgggaca gcacctggat gacccggcgg atctcgtcgt cgcggccgat caccggatcg
     4561 agcttcccgg accgggccag ctcggtgagg tccctgccgt accgctccag ggcctggtac
     4621 ttctcctccg ggttggggtc ggtgacccgc tggcggcccc gcaccttcgc cagggcctgg
     4681 aggacaccgt cccgggtcag gccggcgtcg gccaccagtc gggccacgcc ggccggcccg
     4741 tcgatcatgg ccaacaggag gtgctcggtg gagacgtatt cgtcggtgag ctgctcggct
     4801 tcggcgaacg cccgctccag gacccggcgc gtggccggcg agagctgggc ctcggagccg
     4861 gaaggcccgt acaccttcgg catgcggtcg agggcctcgt ccacccggtc ccggagcgcg
     4921 cgagggctca ggcccatcgc gtgcagcaac gggtacggaa ccccttcggg gtcggacagc
     4981 agcgcgaaca gcacgtgctc gggctcgatg ttctggtggt ttcgcgcgcc ggcctgcgcc
     5041 gaggcggcct ccagcgcggc ccgcgacttc atcgtcagct tgttcaggtc catgtcgttc
     5101 gttcgttctc ccagtcgtcc ttggtcatcg cgtacatggc gtagtcccgt cgtccctgcc
     5161 cggtcggcat gaacgaccgg agcacgccct ccatgcggaa ccccacccgt tcggccaccg
     5221 cccgcatcgc ccggttctcg acgtccgtgg tgagcgacac ccggtgcgcc gattcctcga
     5281 tgaacagccg gtcgcacatc aggcggagcg cctcccggcc gtggccgcgc ccccggtcgg
     5341 cccgcgagaa cacggtgatg ccgagctccc acaccccggg cgggagggcc cacctcgggt
     5401 gccggccgtc gatctccccc accatgcggc cgtcaacctc gatcacgagg tcgatccggc
     5461 cgtcggacag cagccccccg gcgtcgaccc gacggcggag ctcctggcgg ggcggcggac
     5521 cgagcgggag ccactcccgt gcctcctcgg tgcgcatcgc cgcctccatg tcctccagct
     5581 cgcccttgcg gtagggccgg agtgtcacgc cggcgcgtcg cagcacgacc ctggtcacag
     5641 gccctcccac ggcatgcggg gcatgctcga t
//
LOCUS       DASPWO010000550         6361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_500836, whole genome shotgun sequence.
ACCESSION   DASPWO010000550 DASPWO010000000
VERSION     DASPWO010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500836"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3864)
                     /locus_tag="VEO00_12685"
     CDS             complement(<1..3864)
                     /locus_tag="VEO00_12685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HYU58884.1"
                     /translation="MLDVNYFDVLKIGLATADQIRAWSNGEVKKPETINYRTLKPEKD
                     GLFCERIFGPTRDWECHCGKYKRVRFKGIICERCGVEITRSKVRRERMGHIELAAPVT
                     HIWYFKGVPSRLGYLLDLAPKDLEKVIYFAAYIITRVEEERRHKDLPEIEAEFADEKK
                     SIEARRDEQIRDRLSLLEKRLRELEGEGAKGAQLTAARREAQRDVDRLTEAARRDIEH
                     LDEVLSEFKGLSRKKLLADDLVYRTLRERFGEYFDGGMGAEAIKRLLSDLDLEAEAEF
                     LREQIRTAKGTRRLKAIKRLKVVTNFIGTKNSPLGMVLDAVPVIPPDLRPMVQLDGGR
                     FATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDALFDNGRRGRPVT
                     GPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKQMALE
                     LFKPFVMKRLVDQNLAQNIKSAKRMVERARPQVWDVLEEVIKEHPVLLNRAPTLHRLG
                     IQAFEPVLVEGKAIQIHPLVCTAFNADFDGDQMAVHLPLSAEAQAEARILMLSTNNIL
                     SPAHGRPIATPTQDMILGIYYLTIANQRPGKDEDIRRFASADEALMALDGGDADLHDI
                     VKVRLPNKRMQEAEGGEEPQPESNGQAPLVETTIGRMILNEAFPEDFPYVNHHVQKGD
                     VAAIVDDCVARYDKAQVERVLDNLKALGFHYSTRAGVTIGVDDVTTPSEKRKILDQHE
                     RDAQKIETQYRRGIITDDERRQELIEIWTRATEEVKDAMEAGFTERNPVYMMAYSGAR
                     GNIMQIRQLAGMRGLVANPRGEIIPRPIKANFREGLSVLEYFISTHGARKGLADTALR
                     TADSGYLTRRLVDVAQEVIVREDDCGTDRGLPVTIGAITPTGEYQALTKGVLDTRAFG
                     RVLSEDVKVKVDGKSVKVAAAGDLLDDTDVERLQEREVERIYVRSVLSCDAEVGVCRL
                     CYGRDLASGRLVDIGASVGIIAAQSIGEPGTQLTMRTFHTGGVAGEDITHGLPRVQEL
                     FEARRPKGEATITELSGTVELEEDEEKKVRRITVTAEDGEQVQYQVSLRARLTVDDGD
                     RVEVGQQLTEGSVNPHEKLRVEGVQALQLHLVEEVQAVYRSQGVVIHDKHIELIIRQM
                     LRKVQIIEGGDTEFLPGEMVDRRRFEEKNAEIVESGGEPASARPMLMGITKAALATDS
                     WLSAASFQETTRVLTDAAISAKSDPLLGLKENVIIGKLIPAGTGMSRYRNLMTRIPEE
                     AMPEWWLARQREQAEEAEGAEGPTFD"
     gene            complement(3886..>6361)
                     /locus_tag="VEO00_12690"
     CDS             complement(3886..>6361)
                     /locus_tag="VEO00_12690"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HYU58885.1"
                     /translation="DNDLTLTRADILATVSYLVKLHAREPDHWDDDIDHFGNRRLRSV
                     GELIQNQVRIGLSRMERVVRERMTTQDVEAITPQTLINIRPVVAAIKEFFGTSQLSQF
                     MDQTNPLTGLTHKRRLSALGPGGLSRERAGFEVRDVHPSHYGRMCPIETPEGPNIGLI
                     GSLSTYGRINPFGFVETPYRKVTGGRVTDQIDYLAADEEDRHIIAQANTPIDGRGNFT
                     EDHVLVRRKGGEIDYVEPNEVDYMDVSPKQIVSVAAALIPFLEHDDANRALMGANMQR
                     QAVPLLRSEAPLIGTGVEGRAAVDAGDVILVENPGTVEEVSADEIVVRERTGALIRYP
                     LHKFTRSNQGTCINQKPVVNVGDRVSKDQVIADGPATDRGELALGKNLLVAFMPWEGH
                     NYEDAIVISERLAKEDVLTSIHIEEHEVDARDTKLGAEEITRDIPNVSEDILKDLDER
                     GIVRIGAEVNPGDYLVGKVTPKGETELTPEERLLRAIFGEKAREVRDTSLKVPHGESG
                     KVIGVRVFSREAGDELAPGVNQLVRVYVAQKRKISDGDKLAGRHGNKGVIAKVLPEED
                     MPFLADGTPVDLVLNPLGVPSRMNLGQVLETHLGWAARMGFRANGTTVEGPAFVATPV
                     FDGAREDEIAQALASSAANDDGQKLVDEHGKALLFDGRSGEPFDVPVTVGYIYIMKLL
                     HLVDDKIHARSTGPYSMITQQPLGGKAQFGGQRFGEMEVWALEAYGAAFALQELLTIK
                     SDDVLGRVKVYEAIVKGENIPEPGIPESFKVLIKEMQSLCLNVEVLSAEGKEIEMRET
                     DEDVFRAAEELGIDLSRREPAGADFD"
BASE COUNT          834 a         2255 c         2126 g         1146 t
ORIGIN      
        1 gtcgaatgtc gggccctccg cgccctcggc ctcctccgcc tgctcgcgct ggcgggccag
       61 ccaccactcc ggcatggcct cctcggggat gcgcgtcatc aggttccggt agcggctcat
      121 gccggtcccg gccgggatga gcttgccgat gatgacgttc tccttcagcc cgagcagcgg
      181 gtcggacttc gcgctgatcg ccgcgtcggt gagcacccgc gtggtctcct ggaacgaggc
      241 ggccgagagc cacgagtcgg tggcgagcgc cgccttcgtg atccccatca gcatcgggcg
      301 ggccgaggcc ggctcgccgc cggactccac gatctcggcg ttcttctcct cgaacctccg
      361 gcggtcgacc atctcgccgg gcaggaactc ggtgtccccg ccctcgatga tctgcacctt
      421 gcgcagcatc tgccggatga tcagctcgat gtgcttgtcg tggatcacga cgccctggct
      481 gcggtagacg gcctggacct cctcgaccag gtgcagctgc agggcctgca cgccctccac
      541 gcgcagcttc tcgtgcgggt tgaccgagcc ctcggtgagc tgctggccga cctcgacgcg
      601 gtcgccgtcg tccaccgtga gccgggcgcg cagtgacacc tgatactgca cctgctcgcc
      661 gtcttccgcg gtgacggtga tccggcggac cttcttctcc tcgtcctcct cgagctccac
      721 cgtcccggac agctcggtga tggtcgcctc gcccttcggg cggcgggcct cgaacagctc
      781 ctgcacccgc ggcaggccgt gcgtgatgtc ctccccggcg acgccgccgg tgtggaacgt
      841 gcgcatggtc aactgggtgc cgggctcgcc gatggactgc gccgcgatga tcccgaccga
      901 ggcaccgatg tccaccagcc gcccggacgc caggtcgcgc ccgtagcaga gacggcacac
      961 gccgacctcc gcgtcgcagg agagcaccga gcggacgtag atccgctcca cttcgcgctc
     1021 ctgcaggcgc tcgacgtcgg tgtcgtcgag caggtcgccg gcggcggcca ccttcaccga
     1081 cttgccgtcc accttgacct tgacgtcctc cgacagcacg cgcccgaacg cgcgcgtgtc
     1141 gagcacgccc ttggtgagcg cttggtactc gccggtgggc gtgatcgccc cgatcgtcac
     1201 cggcaggccg cggtcggtgc cgcagtcgtc ctcgcggacg atgacctcct gggccacgtc
     1261 caccaggcgc cgggtcaggt accccgagtc cgcggtgcgc agcgcggtgt cggccaggcc
     1321 cttgcgggcg ccgtgcgtcg agatgaagta ctcgagcacg gagaggccct cccggaaatt
     1381 ggccttgatc ggccgcggga tgatctcgcc gcgcgggttg gccaccaggc cgcgcatccc
     1441 cgccagctgg cggatctgca tgatgttccc gcgcgccccg gagtaggcca tcatgtagac
     1501 cggattgcgc tcggtgaagc ccgcctccat ggcgtccttc acctcttcgg tcgcccgcgt
     1561 ccagatctcg atcagctcct gccggcgctc gtcgtcggtg atgatcccgc ggcggtactg
     1621 ggtctcgatc ttctgggcgt cgcgctcgtg ctggtcgagg atcttgcgct tctcgctcgg
     1681 ggtggtgacg tcgtcgacgc cgatcgtcac gccggcccgc gtggagtagt ggaagccgag
     1741 cgccttcagg ttgtccagca cccgctccac ctgcgccttg tcgtagcgcg ccacgcagtc
     1801 gtcgacgatc gccgccacgt cgcccttctg gacgtggtgg ttcacgtacg ggaagtcctc
     1861 cgggaaggcc tcgttcagga tcatccgccc gatcgtcgtc tcgaccaggg gagcctggcc
     1921 gttcgactcc ggctgcggct cctcgccccc ctcagcctcc tgcatccgct tgttcgggag
     1981 gcgcaccttg acgatgtcgt gcaggtccgc gtcgccgccg tcgagcgcca tcagcgcctc
     2041 gtcggcggac gcgaagcgcc ggatgtcctc gtccttcccc ggtcgctggt tggcgatcgt
     2101 caggtagtag atccccagga tcatgtcctg ggtcggggtg gcgatcgggc ggccgtgcgc
     2161 cggcgacagg atgttgttcg tcgagagcat caggatccgc gcctcggcct gcgcctccgc
     2221 ggacagcggg aggtgcacgg ccatctggtc gccgtcgaag tccgcgttga acgccgtgca
     2281 gacgagcggg tggatctgga tggccttgcc ctccacgagg accggctcga aggcctggat
     2341 ccccaggcgg tgcagcgtcg gcgcgcggtt caggagcacg gggtgctcct tgatgacctc
     2401 ctcgagcacg tcccacacct gcgggcgcgc ccgctcgacc atccgcttcg cgctcttgat
     2461 gttctgggct aggttctggt cgacgagccg cttcatcacg aacggcttga agagctcgag
     2521 cgccatctgc ttcggcaggc cgcactggtg cagcttcagc tcagggccga ccacgatcac
     2581 cgagcggccg gagtagtcca cgcgcttgcc gagcaggttc tggcggaagc gcccctgctt
     2641 gcccttgagc atgtccgaca gcgacttcag cgggcggttg ccggggcccg tcaccggccg
     2701 gccacgccgg ccgttgtcga acagcgcgtc caccgcctcc tggagcatcc gcttctcgtt
     2761 gttgacgatg atctccggcg cgccgaggtc gagcagtcgc ttcaaccggt tgttgcggtt
     2821 gatgacgcgg cggtagaggt cgttgaggtc cgacgtggcg aaccggccgc catcgagctg
     2881 caccatcggg cgcaggtcgg ggggaatcac cggcaccgcg tcgaggacca tccccagcgg
     2941 ggagttcttc gtgccgatga agttcgtgac gaccttcagg cgcttgatcg ccttcagccg
     3001 ccgagtgccc ttcgccgtgc ggatctgctc gcgcaggaac tccgcctcgg cctcgaggtc
     3061 gaggtcggac agcaggcgct tgatcgcctc ggcgcccatg ccgccgtcga agtactcgcc
     3121 gaagcgctcg cgcagcgtgc ggtagaccag gtcgtcggcc agcagcttct tccggctcag
     3181 gcccttgaac tcggacagca cctcgtcgag gtgctcgatg tcgcgcctgg ccgcctcggt
     3241 caggcggtcg acgtcgcgct gcgcctcgcg ccgggcggcg gtcagctggg cacccttcgc
     3301 gccctcgccc tccagctcgc gcaggcgctt ctcgagcagg ctgagccgat cacggatctg
     3361 ctcgtcgcgg cgggcctcga tcgacttctt ctcgtcggcg aactccgcct cgatctcggg
     3421 gaggtccttg tgccggcgct cctcttcgac gcgcgtgatg atgtaggccg cgaagtagat
     3481 gaccttctcg aggtccttcg gcgccaggtc gagcaggtag ccgagccggc tggggacgcc
     3541 tttgaagtac cagatgtggg tgaccggcgc ggccagctcg atgtggccca tccgctcgcg
     3601 gcggaccttg gagcgggtga tctccacgcc acaccgctcg cagatgatgc ccttgaagcg
     3661 gacgcgcttg tacttcccgc agtggcactc ccagtcgcgg gtgggaccga agatccgctc
     3721 gcagaacagg ccgtccttct ccggcttcag cgtgcggtag ttgatggtct ccggcttctt
     3781 gacctcgccg ttcgaccacg cgcggatctg gtccgccgtg gccaggccga tcttcaggac
     3841 gtcgaagtag ttgacgtcaa gcactgcgct atcgcctcct cggcctcagt cgaagtccgc
     3901 tccggccggc tcgcgccggg agaggtcgat cccgagctcc tccgcggccc ggaacacgtc
     3961 ctcgtcggtc tcgcgcatct cgatctcctt gccctcggcc gagaggacct cgacgttcag
     4021 gcacagggac tgcatctcct tgatcagcac cttgaacgac tcggggatcc ccggctcggg
     4081 gatgttctcg cccttgacga tggcctcgta caccttcacc cggccgagga cgtcgtcgga
     4141 cttgatcgtc aggagctcct gcagcgcgaa ggccgcgccg taggcctcga gcgcccagac
     4201 ctccatctcg ccgaaccgct ggccgccgaa ctgggccttt cccccgagcg gctgctgcgt
     4261 gatcatcgag tacgggccgg tgctccgggc gtggatcttg tcgtccacca ggtggagcag
     4321 cttcatgatg tagatgtagc cgacggtcac cggcacgtcg aagggctctc cggagcgccc
     4381 gtcgaacagc agcgccttcc cgtgctcgtc gacgagcttc tgcccgtcgt cgttcgccgc
     4441 cgacgacgcc agggcctggg cgatctcgtc ctcgcgggcc ccgtcgaaca ccggggtggc
     4501 cacgaacgcc ggcccctcga cggtggtgcc gttcgcccgg aagcccatcc tcgccgccca
     4561 cccgaggtgg gtctcgagga cctgcccgag gttcatccgg gacggcacgc cgagcgggtt
     4621 caggaccaga tcgacggggg tcccgtcggc gaggaacggc atgtcctcct ccgggagcac
     4681 cttggcgatc acgcccttgt tcccgtggcg gccggcgagc ttgtcgccgt ctgagatctt
     4741 tcgcttctgg gccacgtaca cccggaccag ctggttgacg cccggcgcca gctcgtcgcc
     4801 ggcctcgcgc gagaacaccc gcaccccgat caccttgccc gactcgccgt gaggcacctt
     4861 cagcgaggtg tcgcgcacct cgcgtgcctt ctccccgaag atcgcgcgca gcagccgctc
     4921 ctcgggggtc agctcggtct cgcccttcgg cgtgaccttg ccgaccaggt agtcgcccgg
     4981 gttgacctcg gccccgatgc ggacgatgcc gcgctcgtcg aggtccttca gaatgtcctc
     5041 cgagacgttc gggatgtccc gggtgatctc ctcggccccg agcttggtgt cgcgcgcgtc
     5101 cacctcgtgc tcctcgatgt ggatcgaggt cagcacgtcc tccttggcca ggcgctcgga
     5161 gatgacgatg gcgtcctcgt agttgtgacc ctcccagggc atgaatgcca ccagcaggtt
     5221 cttgccgagc gccagctcgc cgcggtcggt cgcgggtccg tcggcgatga cctggtcctt
     5281 cgacacccgg tcgccgacgt tcaccaccgg cttctggttg atgcaggtgc cctggttgga
     5341 gcgcgtgaac ttgtgcaggg ggtagcggat cagcgcgccc gtgcgctccc ggaccacgat
     5401 ctcatcggcc gagacctcct cgacggtgcc cgggttctcg accaggatca cgtcgccggc
     5461 gtccaccgcc gcccggccct cgacgccggt cccgatcagc ggcgcctcgg agcgcaggag
     5521 cggcaccgcc tggcgctgca tgttcgcccc catcagggcc cggttggcgt cgtcgtgctc
     5581 gaggaacggg atcagggccg ccgccaccga cacgatctgc ttcggggaga cgtccatgta
     5641 gtcgacctcg ttcggctcga cgtagtcgat ctcgccgccc ttgcgccgga cgagcacgtg
     5701 gtcctcggtg aagttcccgc gcccgtcgat cggcgtgttc gcctgagcga tgatgtggcg
     5761 gtcctcctcg tcggcggcga ggtagtcgat ctggtcggtg acacggccgc ccgtgacctt
     5821 gcggtacggc gtctcgacga acccgaacgg attgatccgc ccgtatgtcg acagcgagcc
     5881 gatcaggccg atgttcgggc cctcgggggt ctcgatcggg cacatccgcc cgtagtgtga
     5941 cgggtgcacg tcgcggacct cgaagccggc gcgctcgcgc gacaggccgc ccgggccgag
     6001 cgccgacagc cggcgcttgt gggtcagccc ggtgaggggg ttcgtctggt ccatgaactg
     6061 cgacagctgc gaggtgccga agaactcctt gatcgccgcg accaccgggc ggatgttgat
     6121 cagcgtctgc ggcgtgatcg cctcgacgtc ctgcgtggtc atccgctcgc ggaccacccg
     6181 ctccatccgg gagaggccga tccggacctg gttctggatc agctcgccga ccgatcgcag
     6241 gcggcggttg ccgaagtggt cgatgtcgtc gtcccagtgg tcgggctcgc gggcgtggag
     6301 cttcaccaga taggagacgg tggccaggat gtcggcccgg gtcagggtga ggtcgttgtc
     6361 g
//
LOCUS       DASPWO010000551         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_418721, whole genome shotgun sequence.
ACCESSION   DASPWO010000551 DASPWO010000000
VERSION     DASPWO010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418721"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /gene="truB"
                     /locus_tag="VEO00_12695"
     CDS             complement(<1..577)
                     /gene="truB"
                     /locus_tag="VEO00_12695"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HYU58886.1"
                     /translation="MTDGFLLVAKPAGITSHDAVDRVRRALGIRKVGHAGTLDPLAEG
                     LLLMGAGRGTRLLRFLADLDKEYEGTARLGIETDTLDAEGTVVATGAVDVDEGALLVA
                     LAALTGAVSQLPPAYSAVKVGGEALYRAARAGRPARAEARVVRVDAFDLLRFATPDVE
                     FRVVCSGGTYVRALVADLGHALGCGAHLTRLV"
     gene            complement(574..1611)
                     /locus_tag="VEO00_12700"
     CDS             complement(574..1611)
                     /locus_tag="VEO00_12700"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HYU58887.1"
                     /translation="MTEPAVAASPFDEAARVLATAEEVALACHVNPDPDAIGSMLGLG
                     LHLAGRGARVVASWGNEPFIRPPFLAALPGDGLIVPPAEFPRAPAVFVAFDTASAERL
                     GALTGALRRAGISICLDHHVTNPGFGTIDVIDHHASSTAELAYRIAGAIGGDLSPDAA
                     ACLYAGILTDTGRFQYEAATPDTLRVAAALRDTGFDHVRLGQALFEDNTVRFLRVTRA
                     ALDRLVLDEEADLIWTYLTQADLAEAGATSMDTDDLIDVVRTAREADVACIVKQQRDG
                     RFKVSLRSRGGHDVGAVAAAFGGGGHRLAAGYTSQSGPEETVRALASELRSHGIAGGS
                     GGIPREPPRAR"
     gene            complement(1604..1996)
                     /gene="rbfA"
                     /locus_tag="VEO00_12705"
     CDS             complement(1604..1996)
                     /gene="rbfA"
                     /locus_tag="VEO00_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HYU58888.1"
                     /translation="MIEDFGGGHRAERVRPERVAEAFKEILAAEIPRLKDPRVGFVTV
                     TGVDVTPDLRRAHVYYTVLGEEPDHRATRAALRSARGHLRRALGGQLRLKYLPELLFE
                     EDAAAARGERVEEILRTLRAEREDGDGD"
BASE COUNT          282 a          806 c          759 g          265 t
ORIGIN      
        1 ggaccagccg ggtgaggtgc gcgccgcacc ccagcgcgtg ccccaggtcg gcgaccagcg
       61 cccggacgta ggtcccgccc gagcagacga cccggaattc aacgtcgggg gtcgcgaagc
      121 gcaggaggtc gaacgcgtcc acgcggacga cgcgcgcctc ggcacgagcc ggccgtccgg
      181 cgcgggcggc gcggtagagc gcctcgcctc ccaccttcac cgcggagtac gccggcggga
      241 gttgggaaac cgcgccggtc agggccgcga gcgccacgag cagcgccccc tcgtcgacgt
      301 ctaccgcccc cgtcgccacc accgtcccct cggcgtcgag cgtgtcggtc tcgatcccga
      361 ggcgcgcggt gccctcgtac tccttgtcca ggtccgccag gaagcgcagc aggcgggtcc
      421 cacggccggc ccccatcagc agcagcccct cggccagcgg gtcgagcgtc ccggcgtgcc
      481 cgaccttgcg gatcccgagc gcccgccgga cgcgatccac ggcgtcgtgc gaggtgatcc
      541 ccgccggctt ggccacgagc aggaatccat cggtcaccgt gctctggggg gttcgcgggg
      601 gatcccccca gacccccccg cgatcccgtg cgatcggagc tctgatgcca gggccctgac
      661 ggtctcctcg ggccccgact gggaggtgta gccggccgcc aggcggtggc cgcccccgcc
      721 gaacgcggcg gcgaccgcgc cgacgtcgtg gccgccccgt gagcgcaggc tgaccttgaa
      781 ccggccgtcc cgctgctgct tcacgatgca ggcgacgtcc gcctcgcgcg cggtgcggac
      841 cacgtcgatc aggtcatcgg tgtccatgct cgtggcgccg gcctccgcca ggtcggcctg
      901 ggtcaggtag gtccagatca gatccgcctc ctcgtcgagg acgagccggt cgagcgccgc
      961 gcgagtcacc cgcaggaatc ggaccgtgtt gtcctcgaac agcgcctgcc ccaggcggac
     1021 gtggtcgaac ccggtatccc gcagcgccgc ggccacccgg agcgtgtccg gtgtggccgc
     1081 ctcgtactgg aagcggccgg tgtcggtcag gatgccggcg tacaggcacg ccgccgcgtc
     1141 gggagacagg tcgccgccga tcgcccccgc gatgcggtag gccagctcgg ccgtcgacga
     1201 ggcgtgatga tcgatcacgt cgatcgtccc gaacccgggg ttcgtgacgt ggtggtcgag
     1261 gcagatcgag atccccgccc ggcggagcgc gccggtcagc gcgccgagtc gctcggccga
     1321 ggccgtgtcg aacgcgacga acaccgccgg cgcgcgcggg aactccgcgg gcgggacgat
     1381 gagcccgtcg ccggggagcg ccgccaggaa cggcgggcgg atgaagggct cgttgcccca
     1441 cgaggcgacg acccgggcac cccgcccggc caggtgcagc ccgagaccga gcatcgaccc
     1501 gatcgcgtcg gggtcgggat tgacgtggca ggcgagcgcc acctcctccg ccgtggccag
     1561 cacgcgcgcc gcctcgtcga acgggcttgc cgccaccgcg ggctcagtca ccatcgccat
     1621 cctcccgctc ggcgcgcagc gtgcgcagga tctcctcgac gcgctcgccg cgggcggccg
     1681 ccgcgtcctc ctcgaacagc agctccggga ggtacttcag gcgcagctgc ccgccgaggg
     1741 cgcggcggag gtggccgcgg gcggaccgca acgcggcacg cgtggctcgg tggtcgggct
     1801 cctcaccgag caccgtgtag taaacgtgcg cgcggcgcag gtccggggtg acgtccacgc
     1861 cggtcacggt gacgaagccg acgcgcgggt ccttcagccg cgggatctcc gccgccagga
     1921 tctccttgaa cgcctccgcg acgcgctccg ggcgaacgcg ctccgcccgg tgcccgccgc
     1981 cgaagtcctc gatcaccggt agtcctccgg gtcgatcagg tcgctggtcc ggccgatcac
     2041 ctcgacgcgg ccgaacgcgc cgacatgctt ctcgacggct tgcagcactc gacggaggtg
     2101 atatccgctc tc
//
LOCUS       DASPWO010000552         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_500946, whole genome shotgun sequence.
ACCESSION   DASPWO010000552 DASPWO010000000
VERSION     DASPWO010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500946"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1137
                     /locus_tag="VEO00_12710"
     CDS             19..1137
                     /locus_tag="VEO00_12710"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HYU58889.1"
                     /translation="MTCKAGPLDLATPSGRMVARQLGAVARYEVEQMIERQRRAKLQA
                     ATDPDGGRWTGGRRPYGYEADGVTVIEAEAACVREACEQVLAGVSLIGTAAELNRRGC
                     RTSTGRPWRATELRRMLLRPRNAGLMQYRGEIVGKAAWPPIVDEDTWRAVVALLTDPA
                     RRTNPGAGARWLLSGMALCGVCGAPVRSTSAGGHSRGRRMKPAYTCRSGKHVVRDAAE
                     VDRFVEKVILERLSRKDARDLLTRDTPGDTAGLHLEADQLDQRLKGLADAYADGAIDA
                     GQLRSGSERLRARQAAVEEEIAARSRRSVLAGVADAPDPAKVWKGLPLDRRRAVVDVL
                     VTVTILPGRRGRRPGWQAGESYFDPATVRVEPRKVNEA"
     gene            1346..1597
                     /locus_tag="VEO00_12715"
     CDS             1346..1597
                     /locus_tag="VEO00_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015627512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58890.1"
                     /translation="MPSPDPKARHHSARIGALSRSRKPDDPDLIEAQRALRVLQAEEY
                     VRRLVDGFPPLTREQRARLATLLQPAEVKEADDAAAGAA"
     gene            1724..2539
                     /locus_tag="VEO00_12720"
     CDS             1724..2539
                     /locus_tag="VEO00_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58891.1"
                     /translation="MSGQLASWVDEHAQVDTDLLKGADVGEKAVVDVVRALALASYPD
                     GTSAYAPTWDELAHRTRTSRSTVAAALKLAVEAKLLSVDRPGRGRGNATVYRVHFTLC
                     RPVDACQAACKGLATAQAEKVQQPDPFQAPTRSRKGPAGRRKGPGGGRKGPAAGPTTE
                     TEERHPSQGGAVPPPESNARAPLRSAASGSAARSAPQEPARSGDAMPGLPNLAPVPAE
                     AWAALGKALPSDGGERFESREHEEREKRRRAQLLLVPSGPDPPCADCPEGTAV"
BASE COUNT          394 a          947 c          912 g          296 t
ORIGIN      
        1 ccgcggagca tcccgacgat gacctgtaag gccggcccgc tcgacttggc gacgccatcg
       61 gggagaatgg tcgcgcgcca gctcggcgcg gtcgcccgct acgaggtcga gcagatgatc
      121 gagcggcagc gccgagcgaa gctgcaggcc gcgaccgatc cggacggtgg gcggtggacg
      181 ggcggccgcc gcccctacgg gtacgaggcc gatggcgtga ccgtgatcga ggcggaggcc
      241 gcctgcgtcc gggaggcatg cgagcaggtc ctcgccggcg tcagcctcat cgggaccgcc
      301 gcggagctga accggcgcgg ctgccgcacc tcgaccgggc ggccgtggcg ggcgaccgag
      361 ctgcgccgga tgctgctgcg cccgcgcaac gccggcctga tgcagtaccg cggcgagatc
      421 gtcgggaagg ccgcctggcc gccgatcgtg gacgaggaca cctggcgggc ggtcgtcgcg
      481 ctgctgaccg acccggcgcg gcgtaccaac ccgggcgccg gcgcccgctg gctgctctcc
      541 ggcatggcgc tctgcggcgt ctgcggcgcc cccgtgcggt cgacgtcggc gggtgggcac
      601 agccgcggcc ggcgcatgaa gcccgcctac acctgccgga gcgggaagca cgtcgtccgg
      661 gacgccgccg aggttgaccg gttcgtcgag aaggtgatcc tggagcgctt gtcccgcaag
      721 gatgcgcgcg acctgctcac ccgcgacacc ccgggcgaca cggccgggct gcacttggag
      781 gctgaccagc tcgaccagcg gctcaagggc ctcgccgacg catacgccga cggcgcgatc
      841 gacgccggcc agctccgctc cggcagcgaa cggctccgcg cccgccaggc ggcggtcgag
      901 gaggagatcg ccgccaggtc gcgcaggtcg gtgctcgccg gcgtcgccga cgcgcccgac
      961 ccggccaagg tctggaaggg cctgccgctc gaccggcgac gcgcggtcgt cgacgtcctc
     1021 gtcacggtga cgatcctgcc ggggcgcagg ggtcgccggc ccggctggca ggcgggggag
     1081 agctacttcg acccggccac cgtgcgggtc gagccgagga aggtcaacga agcgtagggc
     1141 atccctgacg ctctgacctg caggaatggc ttgcatgcgg gctcatgcgg tgcgacacta
     1201 gaccgcaacg acgaatgagc gcatagcccg aagaagcagt agcgggacgg ccgcggcccc
     1261 atcatcaccg cggccgaaga aggagtaccg agcaggcaac gcgctccttg acgccgcgcc
     1321 gtgatgcgcg gccgaggagg cttcggtgcc ctctcccgat cccaaggcgc ggcaccacag
     1381 cgcccgcatc ggtgcgctct cacgtagccg caagcccgac gatcccgatc tgatcgaggc
     1441 gcagcgtgcc ctccgggtct tgcaggctga ggagtacgtc cggcggctgg tcgacgggtt
     1501 cccgccgctg acccgcgagc agcgcgcccg gctcgcgacc ctgctccagc cggccgaggt
     1561 gaaagaggcg gacgatgccg ctgcgggcgc cgcctgatgc gcgaagccgc cggcttgggc
     1621 aaccggcggc tcccgacaac ctcaccctcg acggatcggt tggcaccatc ctatccgccc
     1681 gtcccgacag gcgtcatgtc cccacggaac cggggaggcg gccgtgagcg ggcagctcgc
     1741 gtcctgggtc gacgagcacg cccaggtcga caccgacctg ctcaagggcg ccgacgtggg
     1801 cgagaaggcc gtcgtcgacg tcgtccgcgc actggccctc gcctcctacc ctgacggcac
     1861 gagcgcctac gcgccgacct gggacgagct ggcccaccgg actcgaacct cgcgaagcac
     1921 cgtcgcagcc gcgctgaagc tcgccgtcga ggccaagctc ctcagcgtcg accggcccgg
     1981 ccgtggccgc ggcaacgcca ccgtctaccg cgtccatttc accctctgcc gtccggtcga
     2041 cgcctgccag gcagcatgca agggccttgc taccgcccag gcagaaaagg tccagcagcc
     2101 ggaccctttc caggcgccga cccgatcccg aaaaggtcca gccgggcgca gaaaaggtcc
     2161 aggtggcggc agaaaaggtc cagcagccgg acccacaacg gaaacggagg aacggcaccc
     2221 ttctcaaggg ggtgccgttc ctccccctga gtccaacgct cgcgcgccgc tccgctccgc
     2281 cgcaagcggc tccgcggcgc gctcggcacc acaagagccg gccaggagcg gggacgccat
     2341 gccggggctg ccgaacctcg ccccggtacc ggccgaagca tgggccgcgc tcggcaaggc
     2401 cctcccgtct gatggcggtg agcggttcga gagccgcgag cacgaggagc gggaaaaacg
     2461 ccgccgcgcc cagctcctcc tcgtcccgtc cgggcccgat ccgccctgcg ctgactgccc
     2521 ggaaggcacc gcggtatgag ccgacggcc
//
LOCUS       DASPWO010000553         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_589245, whole genome shotgun sequence.
ACCESSION   DASPWO010000553 DASPWO010000000
VERSION     DASPWO010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589245"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1264
                     /locus_tag="VEO00_12725"
     CDS             206..1264
                     /locus_tag="VEO00_12725"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYU58892.1"
                     /translation="MAATREEAPVRYYARATVGVVLVLIAFSIALLVRNVLVLVLIAA
                     VLAMGLEPAVRFMMRRLKMRRPWAVTTVILIGVGVVILLLVLIVPPFVREIRNFARDI
                     PGFIDRLQNASGLVGDLEARFHLSERLRELAANIPNAISGSFSTIFGVTKSLAATTFN
                     VLTVFVLTIYFMGRLPNIRDGIVALMPAAKRGSYANTLDESIRRISGYVSGNIVISLI
                     AGGVSFVFLIVLGIPFAAALAVIVAFTDLIPTIGAILGMVVCAAVAFFSGVVPGVITL
                     IFFTVYQQVENYVIGPRVMRDAVDLSPIAVVLSVLIGGALLGFVGGLLALPVAAAAKV
                     VIRDLWLRERLEKIRATT"
     gene            1341..2426
                     /locus_tag="VEO00_12730"
     CDS             1341..2426
                     /locus_tag="VEO00_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HYU58893.1"
                     /translation="MRDLDSLLRALFDLRGSDLHLKAGAPPLVRVDGLLAPLEGEPVV
                     TAEETEEVLRAIVPAGRWDRFERNHEADFAYQISDLARFRVNAFSQREAVSLVFRLVR
                     VGSPSFEELGLPPVIRQLAVQPRGLVLLTGPTGTGKTTTLAAMIDHINATRRCHILTI
                     EDPIEVIHIDRMASVNQREVGVDTESFVSAMRTAMRQDPDVILIGEMRDMETVQAALA
                     AAETGHLVFSTLHTIDAAETVNRIVDFFPPYQQQQIRVTLSGALRGIVCQRLLPRAGG
                     GRAPAVEVMVNNGRTAECILDPMRTAQINDIVAEGGFYGMQTFDQALLGLVLDGTVTI
                     HDAFDAATNRHDFKLALEQAGVSVPVG"
BASE COUNT          361 a          926 c          889 g          428 t
ORIGIN      
        1 ctccccctgc cgcccggccg cttcacgctc gtcgccgaga gcctgatcca gaacgggggg
       61 ctgccggcgg ggaagccgct cgacgtcgag gtccgggccc acgcgttcag cgaggtcacg
      121 ctgctcctcg acaccgggct ccgatagccg cgcaatcgag cggcgggtgc tggaatcgcc
      181 ggggcgcgcg ggcgagactc cacccgtggc ggcgactcgt gaggaagccc cggtccggta
      241 ctacgcccgc gccacggtcg gcgtcgtcct cgtcctgatc gccttctcga tcgcactcct
      301 ggtgcgcaat gtcctggtgc tggtgctgat cgccgccgtg ctggcgatgg ggctcgagcc
      361 ggccgtgcgg ttcatgatgc gccgcctgaa gatgcgccgg ccgtgggcgg tgaccacggt
      421 gatcctgatc ggcgtgggcg tcgtcatcct gctgctcgtc ctgatcgtgc cgccgttcgt
      481 acgcgagatc cgcaacttcg cccgggacat ccctgggttc atcgatcgcc tgcagaacgc
      541 cagcgggttg gtcggcgacc tggaggcccg gttccacctg tccgagcggc tgcgcgagct
      601 ggcggcgaac atcccgaacg ccatctccgg ctccttctcg acgatcttcg gcgtgacgaa
      661 gagcctggcg gcgaccacct tcaacgtgct gacggtcttc gtcctcacga tctacttcat
      721 gggccggctg ccgaacatcc gcgacggcat cgtggcgctc atgccggcgg cgaagcgcgg
      781 cagctacgcg aacaccctcg acgagtcgat ccgtcggatc tccggctacg tctcggggaa
      841 catcgtcatc tcgctgatcg ccggcggagt ctcgttcgtg ttcctgatcg tcctcgggat
      901 ccccttcgcc gccgcgctgg cggtgatcgt ggccttcacc gacctgatcc cgacgatcgg
      961 ggcgatcctc gggatggtgg tctgcgcggc ggtcgccttc ttctcgggcg tggtcccggg
     1021 cgtgatcacg ctgatcttct tcacggtcta ccaacaggtc gagaactacg tgatcgggcc
     1081 gcgggtgatg cgcgacgccg tggacctgtc gccgatcgcg gtggtcctct ccgtgctgat
     1141 cggcggggcc ctgctcgggt tcgtcggcgg gctcctggcg ctgccggtgg ccgccgcggc
     1201 gaaggtggtc atccgcgacc tgtggctcag ggaacgcctg gagaagatcc gggccaccac
     1261 ctgacccggt cttcgggcag gtgccctgtc ccgacgcacg agcgcctaaa gatcgccccc
     1321 gggcgcgccg aaggaagggt gtgcgagacc tcgactccct gttgcgcgcc ctgttcgacc
     1381 tgcggggctc cgacctccat ctgaaggccg gggcacctcc gctggtgcgc gtcgatggcc
     1441 tgctcgctcc gctggagggc gagccggtcg tcaccgccga ggagaccgaa gaggtcctgc
     1501 gggccatcgt cccggccggg cgctgggacc gcttcgagcg caaccacgag gcggacttcg
     1561 cctaccagat ctccgacctg gcccgcttcc gtgtgaacgc gttcagccag cgggaggccg
     1621 tttcgctggt gttccggctg gtgcgcgtcg gcagcccctc gttcgaggag ctcggcctgc
     1681 cgccggtgat ccgccaactc gccgtccagc cgcgcggcct ggtgctcctg acgggcccga
     1741 ccgggaccgg gaagacgacc accctcgcgg cgatgatcga ccacatcaac gccacccgcc
     1801 gctgccacat cctgacgatc gaggacccga tcgaggtcat ccacatcgac cggatggcgt
     1861 cggtcaacca gcgcgaggtg ggcgtggaca ccgagagctt cgtctccgca atgcgcaccg
     1921 cgatgcgcca ggaccccgac gtgatcctga tcggcgagat gcgagacatg gagaccgtgc
     1981 aggcggcact cgcggccgcc gagaccggac acctggtgtt ctcgaccctg cacacgatcg
     2041 acgccgcgga gaccgtcaac cggatcgtgg acttcttccc cccgtaccag cagcagcaga
     2101 tccgtgtgac gctctccggc gccctgcgtg ggatcgtctg ccagcgcctg ctccctcgag
     2161 ccggaggcgg ccgcgcgccg gccgtggagg tcatggtcaa caacgggcgc acggccgagt
     2221 gcatcctcga cccgatgcgc acggcgcaga tcaacgacat cgtcgccgag ggcggcttct
     2281 acggcatgca gacgttcgac caggccctgc tcgggctcgt gctcgacggg acggtgacga
     2341 tccacgacgc gttcgacgcc gccaccaacc gtcacgactt caagctggcc ctggagcagg
     2401 ccggggtgtc cgtcccggtc ggctaacgcg tcctccaccg ccgggaagat ccgcgatgcg
     2461 ggtacgctct cctaccggga tgccatcggt ggctgatctg gtcgacgagg agacggtccg
     2521 gcgcctggcc gggccggagg cgcacgcccg cgggacgacg ctcgccgacg agggccacgt
     2581 gcggatcggc gaattcgggc cgct
//
LOCUS       DASPWO010000554         3419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_757397, whole genome shotgun sequence.
ACCESSION   DASPWO010000554 DASPWO010000000
VERSION     DASPWO010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757397"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..1348
                     /locus_tag="VEO00_12735"
     CDS             323..1348
                     /locus_tag="VEO00_12735"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYU58894.1"
                     /translation="MPTSPADRPARWATLARNFADVSPFYFNSRESITADPFDSTYVY
                     AVWNRGRKPGDAHSASAEHSFAFRGDTMFSRTTDGGQTWEEPRAIVMYQSNSGTFANQ
                     LAVLPDGTLLNIFDNLQSVGHGRNRYDIKVVISTDRGETWSDPIEVALERAIAPVNPD
                     TGTSVFVDLGRPDLAVDLNPSSPGYGNIYAAWAASFGSPKKTPYSNVVFTESTDGGLT
                     WSPLVKVNRSPAGVQAFTPAVDVASDGTVAVTHHDFRNNTPEPGVPTDLWMIHCHAAT
                     ECTDPANWEENHVAGSFDIERAPRLRGYYLGDYTGLTSSGTTFLAFFAQTTDTDQCNT
                     YLGIVDP"
     gene            1775..>3419
                     /locus_tag="VEO00_12740"
     CDS             1775..>3419
                     /locus_tag="VEO00_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYU58895.1"
                     /translation="MLVCSSCGKENPEGFAFCGYCAAPLPDRPQPPAFEERKVVSVLF
                     CDLVGFTAASERQDPEDVRARIRPYHARLRQEIERYGATVEKFVGDAVMAVFGAPVAH
                     EDDAERAVRAGLRILEAIGELNEADPELDLQVRVGINTGEAVVALGARPEQGEVFVTG
                     DVVNTAARIQSAGPVNGVAVGEQTYRTTRHVFEYERLAPVSVKGKAEPLPLWQATAVR
                     VRFGSDVIRQHSTPLVGRELEKPLLIGTFERAAQQGSVQLVTVVGEPGVGKSRLVAEL
                     FAYIDAKQALTRWRQGRCLPYGEGITFWALGEIVKGEAGILESDSAQVAAAKLEACVS
                     PEEPERRWLLERLAPLVGVEAASPAERQELFTAWRRFLEGLAAAQPTVLVFEDLHWAD
                     EALLAFLEYLAEWSQGVPLLVLCAARPELYERRPGWGAGQRNAHTINLSPLSDQETAE
                     LVSQLIPTTVLSHELEQAILERAGGNPLYAEEFVRLLADRGLEPRTDRLAETALPESL
                     QALIAARLDTLSAERKSLLQDAAILGKVFWAGAVAEIGGR"
BASE COUNT          545 a         1161 c         1199 g          514 t
ORIGIN      
        1 ccggccgacg gcggcagccc attcgcggac tgtgacgtct ccgggttcta cctgcctggg
       61 gagacgagct acctgaacgc cgaggtctcg ccgtgggtgg cggtcaatcc caccgatccc
      121 agcaacatca tcggagtcta ccagcaggac cagttctcgt tcggcagcgg gcgggggctc
      181 gtggcggcgg tgagccatga tggcggccag acgtggtcgg ccacgttccc gcccttcacc
      241 atctgcgccg gcggagacgg gaccaacggg ggcgatttcc agcgatcgcg cgagccggag
      301 gtgacgttct caccgaccgg cgatgcctac ttcaccagcc gatcggccag ccaggtgggc
      361 aacgctcgct cgcaacttcg ccgatgtttc accgttctac ttcaactcca gggagtcgat
      421 cacggcggac ccgttcgact cgacctacgt ctacgcggtg tggaaccgcg gccggaagcc
      481 gggcgatgcc cacagcgcga gcgccgaaca ctccttcgcc ttccggggag acacgatgtt
      541 ctcgagaacc accgacggcg gtcagacgtg ggaggaaccg agggcgatcg tgatgtacca
      601 gagcaactcg gggaccttcg cgaaccagct cgcggtgctg ccggacggga ccctgctcaa
      661 catcttcgac aatctccaga gtgtcgggca cggccgcaac cgctacgaca tcaaggtggt
      721 catctcaacg gatcgtggcg agacatggtc ggatccgatc gaggtggcgc tggaacgggc
      781 catcgccccc gtcaacccgg acacgggcac gtccgtcttc gtggacctcg ggaggccgga
      841 cctcgccgtc gacctcaatc cctcgagccc cgggtacggc aacatctacg ccgcatgggc
      901 ggccagcttc ggcagcccga agaagacccc ctactccaac gtggtgttca ccgagtcgac
      961 cgacggcggg ctcacctggt cgccgctggt caaggtcaac cggtcgccgg ccggcgtgca
     1021 ggcattcacg ccggcggtgg acgtggcgtc cgacggaacg gtggcggtga cccaccacga
     1081 cttccgcaac aacacccccg agcccggcgt cccgacggac ctatggatga tccactgtca
     1141 cgccgcgacc gagtgcaccg acccggcgaa ctgggaagag aaccacgtgg cgggatcgtt
     1201 cgacatcgag cgagccccga ggcttcgcgg gtactacctc ggcgactaca cggggctgac
     1261 gtcgagcggc accacgttcc tggcgttctt cgcccagacc acggacacgg atcagtgcaa
     1321 cacgtacctg gggatcgtcg acccgtagct gcagccgacg cggaaggcag ccggagcacc
     1381 cggtttcgag accggtccaa tgtgttgccc gactcggctg ctccccaccg accggcgaac
     1441 acgtccatgc catggtcccc acggccttcg accaggtcgg aggcgtagga cccgaaggcc
     1501 acgaacccgc cccccgtgct gatggggggc ccgtccccgt cgtagcggct gtcgtcgttc
     1561 gcatccccgc cgtcccggtc cacgctgacc cggacggtga cccccacgag aacatcgcgt
     1621 acgaagacat catcgaaacc tttcttgtca tcctggacca ggtctggggc cgatgaccag
     1681 aacgccacgc agctcccgtc gtctggcgcg agatgaagcg tttgactgag gacgctccgg
     1741 ccgtgggagt gggggtgtca tctacactcg tggtgtgttg gtctgctcga gctgcggcaa
     1801 ggagaacccg gaagggttcg cattctgcgg ctactgcgcg gcgccgcttc ccgacaggcc
     1861 acagccaccc gcgttcgagg agcggaaggt cgtctccgtc ctcttctgtg acctggtcgg
     1921 gttcaccgcc gcctccgaac gtcaggaccc ggaggacgtg cgcgcccgca tccgtcccta
     1981 ccatgcccgt ctgcggcagg agatcgagcg ctacggcgcc acggtcgaga agttcgtcgg
     2041 cgacgcggtc atggccgtgt tcggcgctcc cgtggcgcac gaggacgacg ccgagcgggc
     2101 ggtgcgggcg ggactcagga tcctcgaagc gatcggcgag ctgaacgagg ccgatcccga
     2161 gctggacctg caggtgcggg tggggatcaa cacgggggag gcggtggtgg cgctgggcgc
     2221 ccgccccgag cagggggagg tgttcgtcac cggcgatgtc gtcaacacgg ccgctcgcat
     2281 ccagagcgcc gggccggtga acggggtcgc ggtcggcgag cagacgtacc ggacgacgag
     2341 gcacgtgttc gagtacgagc ggctcgcgcc ggtttccgtg aaggggaagg ccgagcctct
     2401 gccgctgtgg caggcgacgg cggtccgcgt gcgcttcggc agcgacgtca tccgtcaaca
     2461 ctcgacgccg ctggtcgggc gcgagctcga gaagccgctc ctgatcggca cgttcgagcg
     2521 ggcggcgcag cagggttccg ttcagctggt cacggtcgtc ggggagccgg gcgtgggcaa
     2581 gagccggctg gtcgccgagc tgtttgcata catcgacgcg aagcaggccc tcacccgctg
     2641 gcggcagggg cgctgcctgc cctacggcga ggggatcacg ttctgggcgc tgggcgagat
     2701 cgtcaagggg gaggcgggca tcctcgagtc ggactcggcg caggttgcgg cggccaagct
     2761 ggaggcgtgc gtctctcccg aggaacccga acggcggtgg ctgctggagc ggctggcgcc
     2821 gctggtcggg gtcgaggcgg cttcgccggc cgagcgacag gagctgttca cggcctggcg
     2881 gcgcttcctc gagggcctgg ccgccgccca gccgaccgtg cttgtgttcg aggacctgca
     2941 ctgggcagac gaggcgctgc tcgcgttcct ggagtacctc gccgagtggt cgcagggggt
     3001 gccgctgctc gtcctctgcg cggcgcggcc ggagctctac gagcgccggc cgggctgggg
     3061 cgccgggcag cgcaacgccc acacgatcaa cctctcgccg ctctcggatc aggagacggc
     3121 cgagctcgtc tctcagctca tccccaccac cgtcctcagc cacgagctcg agcaggcgat
     3181 cctcgagcgg gcgggcggga acccgctcta cgcggaggag ttcgtgcgcc tgctcgcaga
     3241 ccgcggcctc gagccgcgga cagaccgcct ggcggagacg gcactgcccg agagtttgca
     3301 ggcgctgatc gccgcccgcc ttgacaccct ttcggccgag cgcaagagcc tgctccagga
     3361 cgcggcgata ctcggcaagg tgttctgggc gggcgccgtg gccgagatcg ggggccgcg
//
LOCUS       DASPWO010000555         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_419091, whole genome shotgun sequence.
ACCESSION   DASPWO010000555 DASPWO010000000
VERSION     DASPWO010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419091"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1133
                     /gene="thrC"
                     /locus_tag="VEO00_12745"
     CDS             84..1133
                     /gene="thrC"
                     /locus_tag="VEO00_12745"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HYU58896.1"
                     /translation="MDWQGVIGQYRDRLPVTDATPVVTLLEGGTPLIDAPRLSDRTGA
                     RVLLKLEGANPTGSFKDRGMTVAMSKAAEEQPKAVICASTGNTSASAAAYAARAGLTC
                     GVVIPEGQIALGKLAQALIHGARVVPVRGNFDAALDVVRALGDGGEVSVVNSINPYRI
                     EGQKTAAFEIVDVLGDAPDAHCLPVGNAGNITAYWAGYVEYADAGRTRRRPRMLGWQA
                     AGAAPLVAGEPVPHPETIATAIRIGNPASWDGAIAARDESEGQIGAVHDHEILDAYRL
                     LATLEGCFVEPASAAAVAGLLRAAAEGFIEAGETVVCTLTGHGLKDPQRAIAEVDVGS
                     AVEPTAAAVAAALGL"
     gene            1178..1930
                     /locus_tag="VEO00_12750"
     CDS             1178..1930
                     /locus_tag="VEO00_12750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYU58897.1"
                     /translation="MTGASAGIGRAFASALAARGDDLVVVARDTARLDQLASELRGAG
                     GVNVEVLPADLTTNDGVAAVEERARARVDLLVNNAGFGTFGKFADLPIEREAQEIALN
                     VLALVRLTHAALPGMLERRGGGVINVSSLACYQPTPMNATYGATKAYVTSFSQALHEE
                     TRGTGVKVMVLCPGFTRTEFQARAEVDSSRVPDFLWQSAEQVVAAALRSYDRGRAVCV
                     PGPLNQAGAAFTSALPAGITRRIAAAVLPRVE"
     gene            complement(1931..>2316)
                     /locus_tag="VEO00_12755"
     CDS             complement(1931..>2316)
                     /locus_tag="VEO00_12755"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYU58898.1"
                     /translation="EVDYMPAGEGAGANYGWARYEGTHPFSGDQIPDTVAPIFEYPNP
                     DEGCSVTGGFVYRGTRIPNLKGAYVFADYCTAELRALVQADGAVVAQRSLGLSSENIQ
                     SFGQDGDGELYVLSAGQGLLRIDPA"
BASE COUNT          328 a          816 c          842 g          330 t
ORIGIN      
        1 gcaaccgtgc acgcgctgcg cgacgtcgag ccggtgcacc gcgtcgggtc ggtgctgcgg
       61 gtgatcggcg acgagccgtg agcatggact ggcaaggagt catcggtcag taccgcgatc
      121 gcctacccgt caccgacgcc acgccggtgg tcacgctgct cgaaggcgga acgccgctga
      181 tcgacgcccc ccgactctcc gaccgcaccg gcgcgcgcgt gctgctgaag ctcgagggcg
      241 ccaatcccac cggctccttc aaggaccggg ggatgacggt ggcgatgtcg aaggcggccg
      301 aggagcagcc caaggcggtg atctgcgcct cgaccgggaa cacgtccgcg tcggcggcgg
      361 cgtatgccgc ccgcgccggg ctcacctgtg gcgtggtgat cccggaaggg cagatcgcgc
      421 tgggcaagct cgcccaggcg ctcatccacg gcgcccgcgt cgtgccggtg cgcgggaact
      481 tcgacgccgc cctcgacgtc gtccgcgccc tcggcgacgg cggcgaggtc tcggtggtga
      541 actcgatcaa cccgtaccgc atcgaaggcc agaagacggc cgcgttcgag atcgtcgacg
      601 tgctcggcga cgcccccgac gcccactgct tgcccgtcgg caatgccggc aacatcaccg
      661 cctactgggc gggatacgtc gagtacgccg acgccggtcg cacccggcgc cggccccgca
      721 tgctgggctg gcaggccgcg ggagcggcgc cgctggtggc gggcgagccg gtgccgcacc
      781 ccgagacgat cgccaccgcc atccggatcg ggaacccggc gagctgggat ggcgcgatcg
      841 cggctcgtga cgagtcggag ggtcagatcg gcgcggtgca cgaccacgag atcctcgacg
      901 cctaccggct gctggccacc ctcgagggct gcttcgtcga gccggcgtcg gcggcggcgg
      961 tggcgggcct gctgcgggcg gcggccgaag ggttcatcga ggcgggggag acggtcgtgt
     1021 gcacgctgac aggtcacggg ctcaaggacc cgcagcgcgc catcgccgag gtcgacgtcg
     1081 gatccgcggt cgaaccgacg gccgcggccg tggcggccgc gctcggcctg tagaaggtcg
     1141 gcctgtagaa gggagccgtt ccatgggcac agcactggtg accggcgcgt ccgccggcat
     1201 cggacgcgcg ttcgcgtcgg cactcgccgc gcggggtgac gacctcgtcg tcgtggcgcg
     1261 cgacaccgcc cggctcgacc agctcgcctc ggagctgagg ggcgccgggg gcgtgaacgt
     1321 cgaggtgctg ccggccgatc tgaccaccaa cgacggtgtt gccgcggtcg aggaacgcgc
     1381 ccgcgcccgc gtcgacctgt tggtcaacaa cgccgggttc ggcacctttg gcaagttcgc
     1441 tgacctcccc atcgagcgag aggcgcagga aatcgcgttg aacgtgctcg cgctcgtacg
     1501 gctcacccac gccgcgctcc cggggatgct cgagcgacgc ggcggcggag tcatcaacgt
     1561 gtcgtcgttg gcctgctacc agccgacccc gatgaacgcg acctacggcg cgaccaaggc
     1621 ctacgtgacg agcttcagtc aggcgctcca cgaggagacg cgtggtaccg gcgtgaaggt
     1681 catggtgttg tgcccgggct tcacccgtac cgagttccag gcgcgggccg aggtcgattc
     1741 gagtcgggtg cccgatttcc tgtggcagtc ggccgagcaa gtggtggcgg ccgccctccg
     1801 cagctacgac cgcggtcggg cggtctgcgt gcccggtccc ctgaaccagg ccggggctgc
     1861 gttcacgagc gcgctcccgg ccggcataac ccgccgtatt gccgcggcag ttctgccccg
     1921 cgtggagtag ttacgccgga tcgatgcgga gcaggccctg gccggcggac agcacgtaga
     1981 gctcgccgtc gccgtcctgg ccgaacgact ggatgttctc cgatgacaat cccaggctcc
     2041 gctgcgcgac caccgcgccg tcggcctgca cgagtgctcg cagctcggcg gtgcagtagt
     2101 cggcgaagac gtacgcgccc ttcaggttcg ggatgcgcgt gccgcggtac acgaacccgc
     2161 cggtcaccga gcagccctcg tcggggttcg ggtactcgaa gatcggcgcc accgtgtcgg
     2221 ggatctggtc accgctgaac gggtgggtcc cctcgtagcg ggcccagccg tagttcgcgc
     2281 ccgcgccctc cccggccggc atgtagtcca cctcct
//
LOCUS       DASPWO010000556         3646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1098554, whole genome shotgun sequence.
ACCESSION   DASPWO010000556 DASPWO010000000
VERSION     DASPWO010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098554"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VEO00_12760"
     CDS             <1..963
                     /locus_tag="VEO00_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYU58899.1"
                     /translation="RSTAMMGGKHMRRSFVHLQLVSVALLWGGAFVAIKDLVGTVSPV
                     TVTTLRFLLTGATYGLLLAIWPPARARIDRRDRGRLLVLAVTGTAGYHLALNTGERFV
                     SAGVASLIVASMPVMVALLSARILRERIGAVKRAGIGLALAGVVLLTLYGTPGTRLEL
                     RNLGGALIAVLAPVCWAIHTVIAKPMASRYGAIPVTAQAVTIGSVLLLPFGAPATLAD
                     LPRLTGGDWAWLAFLAFGCTVYAYLIWNAALRRMEASTVAAYVYLVPVSSLAWAAIVL
                     GERLTAFAAFGGALVLAGVILTERGTGWLEARRQPHEEAVKDAA"
     gene            960..1901
                     /locus_tag="VEO00_12765"
     CDS             960..1901
                     /locus_tag="VEO00_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HYU58900.1"
                     /translation="MKILVGGTGGVGGYYGARLLAAGNDVWFMARGRTLDALQERGLT
                     LSSDLGDLAFASVNAVEAGANAGIADLVLFCVKTYDNEPGAGAVAGAVGRRTLVLSLQ
                     NGVENEAFLADRFPRADVLAGVARVEAWVEEPGLIRQRGPQTNLTIGPIEGEPSGRTR
                     DLAAAIADAGVPCEASADMPTALWWKLLGICGVGGVTAYCRCPIGEVLRDPELNDLIT
                     GCWNEAFHVARATGVALPDDTVATMTHYATQVLNLGLKSSMCRDVERGRPLEVEAING
                     AVTRFGKEAGIETPANRQVLDTLLPLHRAAMARRRGG"
     gene            1894..2958
                     /locus_tag="VEO00_12770"
     CDS             1894..2958
                     /locus_tag="VEO00_12770"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYU58901.1"
                     /translation="MADRAHLRIAGIPIRFDPTFFFIVALLGLTARPEPAFVAAWVAV
                     VLVSILLHEMGHALTFRAFGYRPQIVLHGMGGATSTRSPEPIPPGRSLLITLAGPGSG
                     LALGGLVLAFVVAIRPVHGELARTVLSDLLWVNIGWSLLNLIPLLPLDGGRVLVSVLD
                     IVTKGRGERPTRYVGIVVAVAAAVWAFSVGLVFAAVLALFFLFENAQALAGERLPPGD
                     RTLMPRIDEAGRALAAGDADGSASTALEALARARSRRARGEAAQVAVRAMRAQLDGGR
                     FEAAATTGEAIFKRIPDPFLAYDIARTWTRAGRRDQGAVWLGEAVRAGYDDLESLRTD
                     PDLATLRGTPVYEDAVRRLS"
     gene            complement(2987..>3646)
                     /locus_tag="VEO00_12775"
     CDS             complement(2987..>3646)
                     /locus_tag="VEO00_12775"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HYU58902.1"
                     /translation="KTIDNDLAATDFTFGFDTAVQIATDAIDRLHSTAESHARVMMLE
                     VMGRNAGWIGLYSGLAGGADVILIPEHPFDINEVCRHLRHRHAVGHLFSIVVVAEGAV
                     PAPGTMELPPYQADEFGRPRLGGISNVIAEEIQKRTGFDTRVTILGHVQRGGTPTAFD
                     RVLATRFGVAAIDLASAEEWGRMVALRGTEIVSVPLDDAVAELKVVPEELYRVAEVFF
                     G"
BASE COUNT          451 a         1337 c         1306 g          552 t
ORIGIN      
        1 aggtccactg ccatgatggg aggcaagcac atgcggcgct cgttcgtcca cctgcagctc
       61 gtctccgtcg ccctcctgtg gggcggcgcg ttcgtcgcga tcaaggacct ggtcggcacc
      121 gtctcgccgg tcacggtgac cacgctccgc ttcctgctga ccggcgccac ctacggactc
      181 ctgctggcga tctggccgcc ggcgcgggcc cggatcgatc gccgcgaccg cggccgcctc
      241 ctggtcctcg ccgtcaccgg cacggccggc taccacctgg cgctgaacac cggcgagcgc
      301 ttcgtctctg ccggcgtggc cagcctgatc gtggcctcga tgcccgtgat ggtcgccctg
      361 ctgtcggccc ggatcctgcg ggagcggatc ggcgccgtga agcgggccgg catcgggctc
      421 gcgctggcgg gggtggtgct gctgaccctc tacgggacgc ccggcacgcg cctggaattg
      481 cgaaacctcg gcggggcgct gatcgccgtg ctcgctccgg tgtgctgggc gatccacacc
      541 gtgatcgcga agccgatggc gtcgcgctac ggcgcgattc ccgtcacggc gcaggccgtg
      601 acgatcggct cggtgctgct cctgccgttc ggagcgcccg ccacgctcgc cgaccttccc
      661 cggctgaccg gcggcgactg ggcctggctc gcgttcctcg ccttcggctg caccgtgtac
      721 gcgtacctga tctggaacgc ggcccttcgc cggatggagg cgtcgaccgt cgccgcgtac
      781 gtctacctcg tcccggtcag ctcgctcgcc tgggccgcga tcgtcctcgg cgagcggctg
      841 acggcattcg cggcgttcgg cggcgccctg gtcctggccg gggtgatcct gacggagcgg
      901 ggaacggggt ggctcgaggc gcgacggcag ccgcacgagg aagccgtgaa ggacgccgct
      961 tgaagatcct cgtcggggga accggcgggg tgggcggcta ctacggcgcg cggttgctcg
     1021 ccgccggcaa cgatgtctgg ttcatggctc gcggccggac cctcgacgcg ttgcaggagc
     1081 gcgggctcac cctgtcctcg gacctcggag acctggcgtt cgcctcggtg aacgccgtcg
     1141 aggccggggc caacgccggg atcgccgacc tggtcctgtt ctgcgtgaag acctacgaca
     1201 acgagccggg tgccggggcc gtggcgggcg ccgtgggacg gcgaacgctg gtcctgtcgc
     1261 tgcagaacgg cgtggagaac gaagcgttcc tggccgaccg cttcccgcgc gccgacgtcc
     1321 tggccggagt ggcccgggtc gaggcctggg tcgaggagcc cggcctgatc cgtcagcgcg
     1381 gcccgcagac caacctgacc atcggcccca tcgagggcga gccgtcgggc cggacacgag
     1441 acctcgcggc cgccatcgcg gacgcgggcg tcccctgcga ggcgagcgcc gacatgccga
     1501 cggccctgtg gtggaagctg ctcggcatct gcggcgtcgg cggcgtgacg gcatactgcc
     1561 ggtgcccgat cggcgaggtg ctgcgcgacc cggagctgaa cgacctgatc acggggtgct
     1621 ggaacgaggc cttccacgtg gcgcgcgcca ccggcgtggc gctcccggac gacacggtcg
     1681 cgacgatgac ccactacgcc acgcaggtgc tgaacctcgg cctgaagtcc tcgatgtgcc
     1741 gtgacgtcga acgcggccgc ccgctcgagg tcgaggcgat caacggcgcg gtcacccggt
     1801 tcgggaagga ggccggcatc gagacgccgg ccaaccggca ggtgctcgac acgctccttc
     1861 ccctccaccg tgccgcgatg gcccgccgcc gtggtggctg accgcgcaca cctcaggatc
     1921 gccgggatcc cgatccggtt cgacccgacc ttcttcttca tcgtcgccct gctcgggctg
     1981 accgcgcgcc cggagccggc gttcgtcgcg gcatgggtgg cggtcgtgct cgtgtcgatc
     2041 ctgctgcacg agatgggcca cgccctcacc ttcagggcct tcggctaccg gccgcagatc
     2101 gtgctgcacg ggatgggcgg cgccacgtcc acgcgttcgc ccgagccgat cccgcccgga
     2161 cgctcgctcc tgatcaccct ggcagggccg gggtccgggc tggcgctcgg cggtctcgtg
     2221 ctggcgttcg tcgtcgccat ccggcccgtg cacggcgagc tggctcggac cgtgctgtcc
     2281 gatctgctgt gggtgaacat cggctggagc ctcctcaacc tgatcccgct gctccccctc
     2341 gacggcgggc gcgtgctggt ctcggtcctc gacatcgtca cgaagggccg cggcgagcgc
     2401 ccgacccgct acgtggggat cgtcgtggcc gtggccgcgg cggtctgggc cttctcggtc
     2461 ggcctggtct tcgccgccgt cctggccctg ttcttcctgt tcgagaacgc gcaggcgctc
     2521 gccggcgaac gcctcccccc cggcgaccgc acgctcatgc cgcggatcga cgaggccggc
     2581 cgagcgctcg cggccggcga cgccgacggc tcggcgtcga ccgcgctgga ggccctggcc
     2641 cgagcgcggt cgcggcgcgc ccggggcgag gcggcgcagg tggcggtccg ggccatgcgc
     2701 gcccagctgg acggcggccg gttcgaggcc gcggcgacga ccggcgaagc gatcttcaag
     2761 cggatcccgg acccgttcct ggcctacgac atcgcccgga cgtggacgcg ggccggccgg
     2821 cgcgaccagg gcgcggtgtg gctcggcgag gccgtccgcg ccggctacga cgacctcgag
     2881 tcgctgcgga cggatccgga cctggcgacc ctgcgcggaa ccccggtcta cgaggacgcc
     2941 gtccgccggc tgtcctgatc acccgcagac ggtcacccgg agacggtcac ccgaagaaga
     3001 cctcggccac tcggtagagc tcctccggga ccaccttcag ctcggcgacg gcgtcgtcga
     3061 ggggcaccga cacgatctcg gtcccgcgca acgcgaccat cctcccccac tcctccgcgg
     3121 aggccaggtc gatcgccgcc acgccgaagc gcgtggccag cactcgatcg aacgcggtcg
     3181 gcgtcccgcc gcgctgcacg tgcccgagga tcgtcaccct cgtgtcgaac ccggtgcgct
     3241 tctggatctc ctcggcgatg acgttcgaga tgccgccgag gcgcggccgg ccgaactcgt
     3301 ccgcttggta gggaggcagc tccatcgttc ccggcgccgg gacggcccct tccgccacga
     3361 ccacgatcga gaacaggtgc ccgacggcat ggcggtgccg cagatggcgg cagacctcgt
     3421 tgatgtcgaa cgggtgctcc gggatcagga tcacgtcggc gcctccggcg agcccggagt
     3481 acaggccgat ccaaccggcg ttgcgcccca tcacctcgag catcatcacg cgggcatggc
     3541 tctcggccgt cgagtgcagg cggtcgatgg cgtcggtcgc gatctggacg gccgtgtcga
     3601 acccgaaggt gaagtccgtt gccgccaggt cgttgtcgat cgtctt
//
LOCUS       DASPWO010000557         3046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1098970, whole genome shotgun sequence.
ACCESSION   DASPWO010000557 DASPWO010000000
VERSION     DASPWO010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098970"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="VEO00_12780"
     CDS             complement(<1..1711)
                     /locus_tag="VEO00_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HYU58903.1"
                     /translation="MPAESPLLSSLNPVQREAVLHTEGPVLVVAGAGSGKTRILTHRI
                     AHLIREKGVSPWAILAITFTNRAAGEMAERVEGLLGERVASGMWVLTFHSACTRILRR
                     EHAHLGIPSSFSIYDEGDTQRVIQMCVKEANLDPKRFPPRGIAAAIGKAKDHLLSPAD
                     VAMEAGGFYERAVADVYAAYQRRLQGAGALDFDDLIVRTVWLFRDHPEVLEHYQERFP
                     YVLIDEYQDTNRAQYHLVNLLAAKHRNLCVVGDADQGVYSWRGATIQNLLDFERDYPD
                     AAVFVLEQNYRSSQNILALANALIEHNLERQPKSLWTEARSGELVVRMRADDEHDEAW
                     WVAGEVARLADEEGYRYGDVAVFYRTNAQSRVLEDVFARAGMSYRVVGGQRYYERKEI
                     KDALAYLRLLVNPADVISARRVVNAPKRGIGEATVAALEAFARDEELTFLEAGERADQ
                     IGVLGTRARGAVAGFVAIMDGLSRLLEEKGSVPRLVEAAFTDTGYLAELEAERTVEAE
                     GRIENLKELVGVAAEFEARSPDGRLGEFLEQVALVTDQDEYEDEDSTVTLMTLHTAKG
                     LEFP"
     gene            1773..2435
                     /locus_tag="VEO00_12785"
     CDS             1773..2435
                     /locus_tag="VEO00_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HYU58904.1"
                     /translation="MRVTDASLAPPKPRWRGRLHQAAFFVALPFVVALVALASGVEAR
                     TAVAVYGIGLLGVFGTSGLYHRRTWGPRALRWMQRLDHAMIYVLIAGTYTPVSLLAMN
                     GTWSYVLLALVWSGAIFGVVIKLSGWRAAPVLGAVLYIALGWAAVLAGPSLIHGLPLA
                     ALILLAAGGVLYTSGALVLRLRKPNPTPAVFGYHEVWHSMTLGASACHYIAILLLVLT
                     VS"
     gene            complement(2428..>3046)
                     /locus_tag="VEO00_12790"
     CDS             complement(2428..>3046)
                     /locus_tag="VEO00_12790"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HYU58905.1"
                     /translation="AIRAVVDAETRSGDESDTRTPSQRRADALGEICRRHLACADRPL
                     VGGERPQVSVLVDLEALQGGAGRAELEEAGPIDAESARRIACDAAVARVITRGPSEPL
                     DVGRRSPVVSPALRRALVVRDRGCTFPGCDRPPGWCDGHHVKHWADGGETSLANTTLL
                     CRRHHRLVHPPNGFRLVMADGRPVFRRPDGSMLEERGPPGLTGVS"
BASE COUNT          390 a         1141 c         1043 g          472 t
ORIGIN      
        1 ccgggaactc cagccccttc gcggtgtgca gcgtcatcag cgtcacggtg gagtcctcat
       61 cctcgtactc gtcctggtcc gtgacgagcg cgacctgctc caggaactct ccgagcctgc
      121 cgtcgggcga gcgcgcctcg aactcggccg ccacgccgac gagctccttg aggttctcga
      181 tccgtccctc ggcctcgacc gttcgctcgg cctccagctc ggcgaggtag ccggtgtcgg
      241 tgaaggcggc ctcgaccagc cgcggaaccg agcccttctc ctcgagcagt cgcgacaggc
      301 cgtccatgat cgcgacgaac ccggccacgg cgccgcgcgc gcgcgtcccg aggacgccga
      361 tctggtccgc ccgctccccg gcctccagga acgtcagctc ctcgtcgcgg gcgaaggcct
      421 cgagcgcggc cacggtcgcc tcgccgatcc cgcgcttcgg cgcgttcacc acgcgccggg
      481 cggagatgac gtcggccggg ttgaccagca ggcgcaggta cgccagcgcg tccttgatct
      541 ccttgcgctc gtagtaccgc tgcccgccga ccacgcggta cgacataccc gcgcgggcga
      601 agacgtcctc cagcacccgt gactgcgcgt tcgtccggta gaacaccgcc acgtcgccgt
      661 agcggtagcc ctcctcgtcg gccagccgtg cgacctcgcc ggcgacccac catgcctcgt
      721 cgtgctcgtc gtcggcacgc atccgcacga ccagctcgcc cgaccgggcc tctgtccaca
      781 gcgacttcgg ctggcgctcc aggttgtgct cgatcagcgc gttggcgagc gcgaggatgt
      841 tctgcgagga acggtagttc tgctccagca cgaacacggc agcgtcgggg tagtcgcgct
      901 cgaagtcgag caggttctgg atcgtcgccc cgcgccacga gtacacgccc tggtcggcgt
      961 cgcccaccac gcagaggttg cggtgcttgg ccgccaacag gttgaccagg tggtactggg
     1021 cgcggttcgt gtcctggtac tcgtcgatca ggacgtaggg gaagcgctcc tggtagtgct
     1081 cgagcacctc tgggtggtcc cggaacagcc acacggtgcg cacgatcagg tcgtcgaagt
     1141 cgagcgcgcc ggccccctgc agccgtcgct ggtacgcggc gtagacgtcg gccaccgcgc
     1201 gctcgtagaa gcctccggcc tccatcgcca cgtcggcagg cgagagcagg tggtccttgg
     1261 ccttcccgat cgcggcggcg atgccccgcg gcgggaaccg cttcgggtcg aggttcgcct
     1321 ccttgacgca catctggatc acccgctggg tgtcaccctc gtcgtagatc gagaacgagc
     1381 tggggatgcc caggtgtgcg tgctcccggc ggaggatgcg cgtgcaggca gagtggaacg
     1441 tcagcaccca catcccgctg gccacgcgct ccccgagcag gccctcgacc cgctcggcca
     1501 tctcgccggc ggcgcggttg gtgaacgtga tcgccaggat cgcccacggc gagaccccct
     1561 tctcgcggat caggtgggcg atgcggtggg tcaggatgcg ggtcttgccg ctcccggcgc
     1621 cggccacgac caggacgggg ccttccgtat gcagcacggc ctcgcgctgc accggattga
     1681 gggaagacag caggggggat tccgccggca ccgcatcgat tctaggaccg tgccttcgga
     1741 gcctgttcgt cggcctgcca gttcgctacc ctatgcgggt gaccgacgcg agcctggccc
     1801 ccccgaagcc gcggtggcgg gggcgtctcc accaggcggc cttcttcgtg gccctcccct
     1861 tcgtcgtggc gctggtcgcg ctggcctccg gggtcgaggc ccgcacggcc gtcgcggtct
     1921 acgggatcgg cctactcggg gtcttcggga ccagcggcct gtaccaccgc cgcacctggg
     1981 gcccgcgcgc cctgcgttgg atgcagcgcc tggaccacgc gatgatctac gtgctgatcg
     2041 ccggaacgta cacgccggtg tcgctcctgg cgatgaacgg cacctggtcg tacgtgctgc
     2101 tggcgctcgt ctggtccggc gccatcttcg gcgtcgtgat caagctcagc ggctggcgcg
     2161 cagcccccgt cctgggggcc gtgctctaca tcgcgctcgg ctgggccgcc gtcctggccg
     2221 ggccgtcgct gatccacggg ctgcccctcg ccgcgctcat cctgctggcg gccggcgggg
     2281 tcctgtacac gagcggcgcg ctggtcctgc gcctgcgcaa gcccaacccg accccggccg
     2341 tcttcggcta ccacgaggtc tggcactcca tgacgctggg cgccagcgcc tgccactaca
     2401 tcgccatcct gctcctggtg ctgaccgtca gctgacaccc gtcaggcccg ggggcccacg
     2461 ctcctccagc atcgagccgt ccggccttcg gaacaccggc cgcccgtcgg ccatcaccag
     2521 gcggaacccg ttcggcgggt gcaccaggcg gtggtggcgc cggcagagca gggtggtgtt
     2581 ggcgagcgag gtctccccgc cgtcggccca gtgcttcacg tgatggccgt cgcaccagcc
     2641 cggcgggcgg tcacagccgg ggaaggtgca gccgcggtcc cggaccacca gcgcccggcg
     2701 aagcgccggg gacaccaccg ggctcctgcg cccgacgtcg agcggctccg aggggccccg
     2761 ggtgatcacc cgcgccaccg cggcgtcgca ggcgatccgc cgggcgctct cggcatcgat
     2821 cggtccggcc tcctccagct cggcgcgtcc cgctccgcct tgcagggcct ccaggtccac
     2881 cagcacgctc acctgggggc gctccccacc gaccaggggg cgatcggcgc aggccaggtg
     2941 ccgccggcag atctcgccga gggcgtcggc ccggcgctgg gagggagtac gggtatccga
     3001 ttcgtcgccg gagcgggtct cggcgtccac caccgcgcgg atcgcg
//
LOCUS       DASPWO010000558         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1263063, whole genome shotgun sequence.
ACCESSION   DASPWO010000558 DASPWO010000000
VERSION     DASPWO010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263063"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..383)
                     /locus_tag="VEO00_12795"
     CDS             complement(84..383)
                     /locus_tag="VEO00_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58906.1"
                     /translation="MDEPLTEVERRALEWLRDEEYARVAGLDHAVRAGLIGRGLAEMV
                     HTRLRGREHVAYTITPRGRAALDAPRSAAPDASRRPGERRHPGAAGAGGRPRRRS"
     gene            complement(401..637)
                     /locus_tag="VEO00_12800"
     CDS             complement(401..637)
                     /locus_tag="VEO00_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58907.1"
                     /translation="MGEHDGRFTEEHRGRMVRWLLRELAETTDEIAKSAGAPGWTDGD
                     WTEIAAAWGKLRRTIEPWLADAERRIGEKGAGAT"
     gene            794..1036
                     /locus_tag="VEO00_12805"
     CDS             794..1036
                     /locus_tag="VEO00_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYU58908.1"
                     /translation="MDSPEQGMSAARALTIEQAAAALGVSLMTVRRRVREGALPAERV
                     RGKFGPEWRVYLEAPAVLSADHPMPRTDHIDPKTSV"
     gene            1251..1406
                     /locus_tag="VEO00_12810"
     CDS             1251..1406
                     /locus_tag="VEO00_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58909.1"
                     /translation="MVERLRAEWYAIEDATLSLTTPLLRKHLNPFRRYHFDLERMRRT
                     LDEPRGR"
BASE COUNT          261 a          801 c          707 g          261 t
ORIGIN      
        1 aacgggcgga attggccctg gttctccccc atgcacgccg tcgccgtacg cccgtgcgca
       61 gcgtgcgttg ccttgccccg gggtcaggag cgcctccggg gacggcctcc cgcccctgcg
      121 gcgccgggat gtcggcgttc gccgggccgc cgggatgcgt cgggcgcggc cgaccgcggc
      181 gcgtccaacg ccgcgcggcc gcgcggggtg atcgtgtacg ccacgtgctc cctgccccgg
      241 agccgcgtgt ggaccatctc cgccaggccg cggccgatga gcccggcgcg cacggcgtgg
      301 tcgagccccg cgacgcgcgc gtactcctcg tcgcggagcc actccagcgc ccggcgctcg
      361 acctcggtca acggctcgtc catcccgccc cagggacgac tcaggtcgcc ccggccccct
      421 tctccccgat gcgccgctcc gcgtcggcga gccagggctc gatcgtccgc cggagcttgc
      481 cccaggccgc ggcgatctcg gtccagtctc cgtccgtcca ccccggcgct ccggcgctct
      541 tcgctatctc gtcggtcgtc tcggcgagct cccgcaggag ccaccggacc atgcggcccc
      601 ggtgctcctc ggtgaaccgc ccgtcgtgct cgcccaacag cctctcctca caccagcgcg
      661 tcgccgcggt cgaaacgccg gcccgatgct tcgctagaag acaggcaccg cagcagacgt
      721 aacaccggat tcgccggcgg cgtcggcggc ctctgacgag cccttaacga ctgggccgac
      781 ataatcgccg gccgtggaca gccccgagca aggcatgagc gctgctcgag cgctgaccat
      841 cgaacaggca gccgccgcgc tgggcgtgtc gctcatgacg gtgcgccggc gcgtcaggga
      901 aggcgccctc cccgccgagc gcgtgcgggg gaagttcggc cccgagtggc gcgtctacct
      961 cgaggcgccg gcggtgctca gcgccgatca cccaatgccg cgtactgacc acatcgaccc
     1021 gaaaacctcc gtttagcgcc ccggccgcca cgacgcccgg cgatccgcgc tcgtgcggcg
     1081 tggaggatcg cgaagccccg cggctcctcc ccgaccgtcg gccggcgcgg ggtgggccgc
     1141 gttcggcgtc ggctgcgcct accgcgccgt ttccggcgct cgggggggag ggcgatggcc
     1201 caggggaggc cgccgaaccg gggcggagtg cgcggtcatc caccgacggc gtggtcgagc
     1261 ggctccgcgc cgagtggtac gcgatcgagg acgcgacgct gtctttgacg acgccgctgc
     1321 tgcgcaagca cctcaacccg ttccggcgct accacttcga tctcgagcgg atgcgccgga
     1381 cactggacga gccacgcggc cgctaaacgg agggtttcag gccgatgtgg ttagtacgcg
     1441 gacgaccgcg atccaccacc cagcccaacg cccgggacct ggagcgagcg gccgagcagc
     1501 tctcgaccgg cgacggtacg tcgcggtcct agcgccgccc cggccgccag ctgagcacgc
     1561 gccgccccgc ccggatgagg acgccggccc cgctgaggcc gcgccggcgg gcgaagcgcc
     1621 acagcagcgc gaggtagcgc atcgcgcacc ccctctaccc tgaaccggct cagtctaccg
     1681 cgtcgcccgg ccggaccgcg ttcgcgcggc acccgacggg acggccgttc acggtctcca
     1741 gacggccgtt cacggtctcc aatcgagggc tctagcgggt catgctcgag gaggactgac
     1801 gagcccccgc cggcgaccgt tgagcgacgg gtggtggcgc cgagaaggac atcccgttcg
     1861 ccgtcgccgc cggacgcacg cggcagatca tgctgcggcc cggacgtggc gcgccagccg
     1921 gtccggcagc ccgcgcagct cgccgatgag gaccgccacc gccccgctcc gcagctgcgc
     1981 ccggccctcc tgctggtcgt accgggctgg ccagctctgg tcggtcggcg
//
LOCUS       DASPWO010000559         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_336529, whole genome shotgun sequence.
ACCESSION   DASPWO010000559 DASPWO010000000
VERSION     DASPWO010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336529"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..403)
                     /locus_tag="VEO00_12815"
     CDS             complement(14..403)
                     /locus_tag="VEO00_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HYU58910.1"
                     /translation="MPGDTRKDLPDTLKRSSKKAQRTYLKTLESAHDQYRSEERAHRT
                     AIDALKHSFEKVGDHWVEKKRMGPSDPQAAQRGPAARTRPRRTFGGVDYYGNTKDELY
                     ERARKLGVPGRSRMTKRQLAEAIGEKQ"
     gene            complement(458..976)
                     /locus_tag="VEO00_12820"
     CDS             complement(458..976)
                     /locus_tag="VEO00_12820"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYU58911.1"
                     /translation="MTHEIAEDLARLQTVLDRSFERAGDHLRSIFDSESFLRLSPAQL
                     AERLTGVRHLAVATVNRRGEPRVSPVDGLFYRAAFHFSTPGSSYRLRHLRERPAISAT
                     HFQGDHHMVVLHGTAVLIGPGHEAFDGIDGYWTEVYGSSAMGLGADIAYVRIDPHAAF
                     AFARNPDAPWPE"
     gene            complement(987..1538)
                     /locus_tag="VEO00_12825"
     CDS             complement(987..1538)
                     /locus_tag="VEO00_12825"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HYU58912.1"
                     /translation="MESENLRGRLLIGGPALFDPNFRRAVVLVGEHSDEGALGVILNR
                     PSETPVRQAVPPLGSLVGPDEPVFVGGPVQGDSAVVIAEFENPSVAGLVAFGSIGFLV
                     GDIEPTHVEGIRRARVFAGYAGWGAGQLEAELQEDGGWLIEEARPEDVFTTEPDRLWE
                     RVVGRRPRPYAFLGTMPFDPSSN"
     gene            1580..>2039
                     /locus_tag="VEO00_12830"
     CDS             1580..>2039
                     /locus_tag="VEO00_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU58913.1"
                     /translation="MTDELDLLRRLDAYLDAAPRTAARPETIGPFTLFVNEGTGWRYY
                     ARPTPGADAFAPADVETVRARQRALAMPESFEWIADLVPGLADAVRAAGMAISEHPAM
                     VLRSETFRPVPAPDGVAVGAAAPFGDLAVVQAVAMVSFGAPGTGRGLAAEE"
BASE COUNT          261 a          747 c          725 g          306 t
ORIGIN      
        1 gctgaccctc cgcctattgc ttctccccga tcgcctccgc cagctgccgc ttcgtcatcc
       61 gcgaccggcc cggaacgccg agcttgcgcg cccgctcgta cagctcgtcc ttcgtgttgc
      121 cgtagtagtc gacgccgccg aacgtccggc gcgggcgggt gcgcgcggcg ggcccgcgct
      181 gcgcggcctg cgggtcggac ggccccatgc gcttcttctc cacccagtgg tcgccgacct
      241 tctcgaacga atgcttcagc gcgtcgatcg cggtgcggtg ggcgcgctcc tcgctgcggt
      301 actggtcgtg ggcgctctcg agcgtcttca ggtacgtgcg ctgggccttc ttcgacgagc
      361 gcttcagcgt gtcggggagg tccttccgcg tatccccggg catgtcgatc gcctccttcc
      421 ggttgcctga aagaggtcat tcccggatcc gatgcgttca ctccggccag ggggcgtcgg
      481 ggttgcgggc gaacgcgaag gcggcgtggg ggtcgatccg gacgtaggcg atgtccgcac
      541 cgaggcccat cgcggaggag ccgtacacct cggtccagta gccgtcgatc ccgtcgaacg
      601 cctcgtggcc cggcccgatg agcacggccg tcccgtgcag caccaccatg tggtggtcgc
      661 cctggaagtg cgtcgcgctg atcgccggcc gctcgcgcag atgccgcaac cggtacgagc
      721 ttcccggcgt gctgaagtgg aaggccgccc ggtagaagag gccgtcgacg ggcgacacgc
      781 gcggttcccc ccgccggttc acggtggcga cggccaggtg gcgcacgccg gtcagccgct
      841 ccgcgagttg ggcgggagac agccgcagga aggactcgct gtcgaagatc gagcgcaggt
      901 ggtctccggc tcgctcgaac gaccggtcga gcaccgtctg cagccgagcc aggtcctcgg
      961 cgatctcgtg ggtcactcga tgagcctcag ttcgacgagg ggtcgaaggg catggtcccc
     1021 aggaatgcgt aggggcgggg gcggcgcccg acgacgcgtt cccacagacg atccggctcg
     1081 gtggtgaaga cgtcctcggg ccgggcctcc tcgatcagcc accccccgtc ctcctggagc
     1141 tccgcctcga gctgtccggc gccccacccc gcgtagccgg cgaacacccg ggcccggcgg
     1201 atcccctcca cgtgcgtcgg ctcgatgtcg ccgaccagga acccgatcga cccgaaggcc
     1261 accaggccgg ccaccgaggg gttctcgaac tcggcgatga ccacggccga gtccccctgc
     1321 actgggccgc cgacgaacac cggctcgtcc ggcccgacga gactgccgag cggggggacg
     1381 gcctggcgca cgggggtctc cgagggccgg ttcaggatca cgccgagcgc gccctcgtcg
     1441 ctgtgctcgc cgaccagcac cacggcgcgg cggaagttcg ggtcgaagag tgccggcccg
     1501 ccgatcagga gccgcccgcg caggttctcg gactccacgt ccctatcttc ccccgttccg
     1561 tccactagcc ttgccgctca tgaccgatga gctcgatctg ctgcgccgcc tggacgcgta
     1621 cctcgacgcg gcaccgcgca cggccgcacg gccggagacg atcggcccct tcaccctgtt
     1681 cgtgaacgag ggcaccggct ggcgctacta cgcgcgcccg acccccgggg ccgacgcgtt
     1741 cgccccggcc gacgtcgaga ccgtgcgggc ccgccagcgc gcgctcgcga tgccggagtc
     1801 gttcgagtgg atcgccgacc tggtaccagg actggcggac gcggtgcgcg cggcggggat
     1861 ggcgatcagc gagcaccccg ccatggtgct tcgctcggag accttccgcc cggtcccggc
     1921 gcccgacggg gtggcggtcg gggccgccgc gcccttcggg gacctggcgg tggtgcaggc
     1981 agtggcgatg gtctcgttcg gggccccggg taccggtcga ggcctggccg ccgaggagg
//
LOCUS       DASPWO010000560         4362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_78949, whole genome shotgun sequence.
ACCESSION   DASPWO010000560 DASPWO010000000
VERSION     DASPWO010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78949"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VEO00_12835"
     CDS             complement(<1..296)
                     /locus_tag="VEO00_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58914.1"
                     /translation="MRAAAVNARWFLAVAAVATALVGAVAGSAAAAPAGPLPPITLPP
                     TPVPLPVEPNELLDSVGDTAEQLVEGVPLPGAAPAPGPAATPAPATTPPVVPPAP"
     gene            complement(293..1969)
                     /locus_tag="VEO00_12840"
     CDS             complement(293..1969)
                     /locus_tag="VEO00_12840"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU58915.1"
                     /translation="MRGAAARIRLALAVAVAAAIPFAPGLSRGERLGLSGLVLGYAAL
                     VLAVEEIGARRDGFPDQALTVLAGVVTILVATVVLPELLGVALVFYGLSVAFASGIGG
                     RRLGLALAAVAVAAAVVANLLAPDHDRVSALALVLFGAVLPALVVATDVLARGRRRTA
                     ERLARLHDALRAVSVTPDLQATLDSIVGASAEPVGAVAVGILLRDGDRLVPAASSSPT
                     RDWMHRDDVDGGPFARAMKRRETVVVPAGTDGLAALVAVPVTLGRDLVGVLAAAFSEQ
                     GGPRREDLTLLETYAEQTSLVIVQAQAYEHERQAAEQLAEADRVKGEFLGMVSHELRT
                     PLTAVKGFVDTVLLHWERLPDDRRRELLGRASGNADELNRLVGQLLDFARIEADRVKL
                     EPQVVTVRVAAEAVVEELAAVLDGHRVEVDVPEGLAVTADPDGLAHVLANLLANAAKF
                     SPPGSGIRVRARDADDMVVISVVDDGPGVAPADRERIFERFYQVPGSTAPRKGTGIGL
                     GIARTLVELHGGRIWAEGEPGQGATFSFTMPAATTPGRVPRGAPAAAAGA"
     gene            2048..2830
                     /locus_tag="VEO00_12845"
     CDS             2048..2830
                     /locus_tag="VEO00_12845"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYU58916.1"
                     /translation="MAPEPTTDAPTPRSKRVRFGWVLEIIGVRALTTAYDWARDQLVG
                     SESEAFRHARQVIRAERLLGIYHEETIQEWFLPYRAFISFWNIFYGSVHFVMPIVALV
                     WLYLKAPARYLRWRNTLFVMLGLGLLGFWLYPLMPPRLLPASYGFVDTRLEYFGLGHA
                     SRDTSEDNLYAAMPSLHIGWATWIALSLWPLVRRWWAKALLVAYPSAQLFCTVVTANH
                     FFLDAVGGWAVLGLAYLLASAPTEWRHRRDARARPRSVTARP"
     gene            2896..3111
                     /locus_tag="VEO00_12850"
     CDS             2896..3111
                     /locus_tag="VEO00_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58917.1"
                     /translation="MTANRPTVRLDLEPPDVALVALALEHLIEHEGSTPIADRAVVLA
                     AYLRLQRHQALRRQDGRRTQGSDAVSP"
     gene            3231..3533
                     /locus_tag="VEO00_12855"
     CDS             3231..3533
                     /locus_tag="VEO00_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HYU58918.1"
                     /translation="MTKTELERAVADAAGVEKRTAAAVLNALEDVVTSNVRKGEPVTI
                     RPGFIKVSRKDVKARPAREGTNPFTGEPTTFKARPASKTAKVTALKKLKDAVGTKR"
     gene            4060..>4362
                     /locus_tag="VEO00_12860"
     CDS             4060..>4362
                     /locus_tag="VEO00_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58919.1"
                     /translation="MVESLVLLATVVAVLAAAVATYGRLQARRSRILSTLKGEHVTIQ
                     VREGGGALRFLYYVEVRVLAVPDMWSGDARVQVDVIDFDDRTEPVLRAPGLLGEWEE"
BASE COUNT          642 a         1577 c         1536 g          607 t
ORIGIN      
        1 ggcgccgggg ggacgaccgg cggtgtggtc gcgggcgcag gggtcgcggc ggggccgggc
       61 gcgggcgcgg cgccgggcag cggaaccccc tccaccagct gctcggcggt gtcgcccacc
      121 gagtccaaca gctcgttggg ctcgacgggg agcgggaccg gtgtcggcgg gagcgtgatc
      181 ggggggagtg gtcccgccgg cgccgccgcg gccgaccccg ccaccgcgcc gacgagcgcg
      241 gtcgcaaccg ccgccaccgc caggaaccac cgcgcgttca ctgccgcggc cctcatgctc
      301 cggccgcggc cgcgggggcg ccccgaggga cgcgtccggg tgtggtggcg gcgggcatcg
      361 tgaacgagaa cgtcgcgccc tgccccggct cgccctcggc ccagatgcgg ccaccgtgca
      421 gctccaccag ggtgcgggcg atgcccaggc cgatgcccgt gcccttgcgg ggcgccgtgc
      481 tcccgggcac ctggtagaag cgctcgaaga tgcgctcgcg gtccgccggc gcgacgccgg
      541 ggccgtcgtc gacgacggag atcaccacca tgtcatcggc atcgcgggcg cggaccctga
      601 tgccgctccc gggcggcgag aacttcgccg cgttcgcgag caggttcgcc agcacgtggg
      661 cgagcccgtc cggatcggcg gtgaccgcca atccctcggg aacgtcgacc tccacccggt
      721 gcccgtccag caccgcggcc agctcctcca ccaccgcctc ggccgccacc cgaacggtca
      781 ccacctgggg ttcgagcttg acgcggtcgg cctcgatgcg ggcgaagtcg agcagctggc
      841 cgaccaggcg gttcagctcg tcggcgttcc cggatgcccg tcccagcagc tcgcggcggc
      901 gatcgtcggg gaggcgctcc caatggagca gcacggtgtc gacgaatccc ttcaccgcgg
      961 tgagcggtgt gcgcagctca tgcgagacca tgcccaggaa ctcgcccttg acgcggtcgg
     1021 cctcggcgag ctgctcggcc gcctggcgct cgtgctcgta ggcctgcgcc tggacgatca
     1081 cgagcgacgt ctgctcggcg taggtctcca gcagcgtcag gtcctcgcgg cggggcccgc
     1141 cctgctcgga gaaggccgcc gccagcacgc cgacgagatc gcggccgagc gtcaccggca
     1201 ccgccacgag ggcggcgagg ccgtcggtgc cggcggggac gaccaccgtc tcccgacgct
     1261 tcatcgcgcg ggcgaacgga cccccgtcga cgtcgtcgcg atgcatccag tcgcgcgtgg
     1321 ggctgctcga ggcggccggc accagtcggt cgccgtcacg caggaggatc ccgaccgcga
     1381 cggcgccgac gggctcggcg ctcgcgccca cgatggaatc cagcgtcgct tgcagatcgg
     1441 gcgtcacgct gaccgcccgg agcgcgtcgt ggagccgggc gaggcgctcg gcggtccggc
     1501 gccgaccgcg agcaagcacg tcggtggcga ctacgagcgc gggcagcacc gccccgaaga
     1561 gcacgagggc gagggccgag acccgatcgt ggtccggcgc gaggaggttg gcgacgaccg
     1621 cggcggcgac cgcaaccgcg gcgagggcga ggccgagccg gcggccgccg ataccggagg
     1681 cgaacgcgac actcagcccg tagaagacga gcgcgacgcc gagcaactcg ggcagcacga
     1741 cagtggccac caagatcgtg acgacgcctg ccaaaacagt gagggcctgg tcgggaaagc
     1801 cgtcgcggcg ggcgccgatc tcctcgaccg ccagcacgag cgcggcgtag ccgaggacca
     1861 gcccgctcag tccgagccgc tcaccgcggc tgaggccggg ggcgaacggg atcgcggccg
     1921 cgaccgcaac cgccagcgcg agccggatgc gtgccgctgc ccctctcatc gagcccccag
     1981 acctgccggg catggaccgt tccggcccgc cattctccca cctggcgtgg ggctaccttc
     2041 gccggccgtg gcccccgaac cgaccacgga cgcacccact ccccgcagca agcgggtccg
     2101 cttcgggtgg gtcctcgaga tcatcggcgt ccgcgccctc accacggcct acgactgggc
     2161 gcgcgaccag ctcgtcggct cggagagcga ggccttccgc cacgcccggc aggtgatccg
     2221 ggccgagcgg ctgctcggga tctaccacga ggagacgatc caggagtggt tcctgcccta
     2281 ccgcgccttc atctcgttct ggaacatctt ctacggcagc gtccacttcg tgatgccgat
     2341 cgtcgcgctc gtctggctct acctgaaggc gccggcgcgc tacctccgct ggcgcaacac
     2401 cctgttcgtg atgctcgggc tcgggctgct cgggttctgg ctgtaccccc tcatgccgcc
     2461 ccggctgctg ccggcgagct acggcttcgt cgacacccgc ctcgagtact tcgggctggg
     2521 ccacgccagc cgggacacca gcgaggacaa cctctacgcc gccatgccga gcctccacat
     2581 cgggtgggcg acctggatcg ccctctcgct gtggccgttg gtacgccgat ggtgggccaa
     2641 ggccctgctc gtggcctatc ccagcgccca gctgttctgc acggtggtca ccgccaacca
     2701 cttcttcctc gacgcggtgg gcgggtgggc cgtgctcggc ctggcgtacc tcctggcctc
     2761 cgcaccgacc gagtggcggc atcgacggga cgcgcgcgcc cgaccgcgtt cggtgaccgc
     2821 gcgtccttga ggtggtcgcg ggcttccggc ctagaatcga cagcttcgcg gtgactgccg
     2881 gatttgggga gcccgatgac cgccaaccgg cccacggtcc ggctcgatct cgagcctccg
     2941 gacgtcgccc tggtcgcgct cgcgctcgag cacctcatcg agcacgaggg ctcgaccccg
     3001 atcgccgacc gggccgtcgt cctcgccgcc tacctgcggc tccagcgcca ccaggctctc
     3061 cgccgccagg acggccgccg aacccaggga tcggacgcgg tctcgccgta gttcccgaag
     3121 cggtcgcttc gggggatact cggatgcccc tccaggacct ggtaggaagc gggcgcccgt
     3181 gagcgcacgt cgcctgctcg ggtggcgccg accaagaagg agacagcagc atgacgaaga
     3241 ccgagttgga gcgcgccgtc gctgacgcgg ccggcgtaga gaagcgcacg gcggcggcgg
     3301 tgctcaatgc gcttgaggac gtcgtcacga gcaacgttcg gaaaggtgag ccggtcacta
     3361 tccgtcccgg cttcatcaag gtgagccgga aggacgtgaa ggcacgcccg gcgcgcgagg
     3421 gcacgaaccc gttcaccggc gagcccacca cgttcaaggc gcgcccggcg tcgaagacgg
     3481 cgaaggtcac cgcgctcaag aagctgaagg acgccgtggg gacgaagcgg taacagtccc
     3541 tggctcagcc cgggcggtgg agacgatggg actcgaaccc acgaccccct gcttgcaaat
     3601 cacgccgggg acgtcgtgat tacggcgcgt cggcacgaac gccatgtcag cacgtacctt
     3661 gagtgcgcgc cagtatccgt gagtgcgcgc agtttccgcg cgtcgtgcgg gacgcgtgcg
     3721 ggaccgggat gcagtccgcg gtcccatctt gcagcggtgt ttcatatcac tggtcaccga
     3781 cgctccggtt cgcgcctgac cttccgtcgt cgccaggcgg tgaagaggat cacggcaagt
     3841 cccaagcaac cgatgattgg gacgccggat agcacccagg cccacctggg accgggcgtc
     3901 tcgtcgtcgt tagggtctgg cttgtagcgg cggttgtgct cctcgagcac gcgctgattc
     3961 cgagcgtccc atcgctcaag taatcccacg atcacgattc ttccacgagg gagcgaaggc
     4021 gcaccgctga gcagatgccg ccgtacagtc gggatcgcga tggtcgaatc ccttgtcctt
     4081 ctcgcaaccg tcgtcgccgt acttgccgct gcagtcgcga catacggacg cttgcaggcg
     4141 cggcgctcgc ggatcctgag cacgctcaag ggcgagcacg tcaccatcca agttcgtgaa
     4201 gggggcggtg cgctccgctt cctctactac gtagaggtgc gagtcctcgc ggttcccgac
     4261 atgtggagtg gcgacgctcg cgtccaggtc gacgtcatcg acttcgacga ccggacggag
     4321 ccggtgctcc gagctcctgg cctcctcggt gagtgggagg aa
//
LOCUS       DASPWO010000561         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_79007, whole genome shotgun sequence.
ACCESSION   DASPWO010000561 DASPWO010000000
VERSION     DASPWO010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79007"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VEO00_12865"
     CDS             <1..686
                     /locus_tag="VEO00_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HYU58920.1"
                     /translation="LGYQFSPRLADLGEARLWRIDPRAVYGGLDAVARHRINVGLIAA
                     NWDDLLRIAGSLTSSRVRASELLRALLGGGRPTAAARAIAELGRVAKTLHLLAYLDDA
                     GYRRRILTQLNRGEARHSLARAVFHGQRGELRQRYREGQEDQLGALGLVVNAIALWNT
                     RYTEAALTHLRGAGVDVAPADVERLSPLGSAHINMLGRYQFTLAEPVLRGELRELRDP
                     TQDQDLLAV"
     gene            complement(862..1269)
                     /locus_tag="VEO00_12870"
     CDS             complement(862..1269)
                     /locus_tag="VEO00_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYU58921.1"
                     /translation="MGATAKPGTWLVLLGLVLGTVLALGAYAAEGRARAQSTPVALEP
                     LPAPAPVTLQAGPGDAGRGRVPYERSCGSCHGNNGRGEAPLHGPLLNAYYRDDRLLAG
                     LIRNGLGTMPGTAAEQLDDQRTADVVAFIRSLP"
     gene            complement(1279..>2380)
                     /locus_tag="VEO00_12875"
     CDS             complement(1279..>2380)
                     /locus_tag="VEO00_12875"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HYU58922.1"
                     /translation="HLLTAFVFGAYKRPRELTWLVGVVLLLATIGLGFTGYLLPWDEK
                     AYWATTVGTSMAGTVPLVGDALLRIVRGGPEVGALTLTRFYGLHLLVLPGLLIVTVAA
                     HLYLVVYLGVSVPPWPWERRSAELPVEEDARYARFKERGHPFWPDLIAQDALVSLAVF
                     VVLVGLAVLAGAPLEPRADPTNTGYVPRPEWYFLWLFELLKYFPGPLEWVGVALVPLV
                     LVVALVVLPFYDRSPRRRPERRPVAMGLGGAAVLAIGGLTVLAAATTPPTTLVERGVR
                     LNAAQVVGRTLYQGTCASCHGERGQGTKDGPPLAQVGALRDAAFIHRYIEDPKALNPG
                     AKMPPTIPEPGGRQELTHEQVEDITQYLLTYR"
BASE COUNT          318 a          918 c          852 g          292 t
ORIGIN      
        1 tgctcggcta ccagttcagc ccgcggttgg ccgacctggg cgaggcccgg ctgtggcgga
       61 tcgacccgcg ggccgtctac ggcgggctcg acgcggtggc ccgccaccgc atcaacgtcg
      121 gcctgatcgc ggccaactgg gacgacctcc tgcgcatcgc cgggtcgctg accagcagcc
      181 gggtccgggc ctccgagctg ctgcgcgcgc tcctcggcgg cgggcggccg acggcggcgg
      241 cgcgcgccat cgccgagctc gggcgcgtcg ccaagaccct ccacctcctc gcctacctcg
      301 acgacgccgg ctaccggcgg cgcatcctca cgcagctcaa ccgcggcgag gcccggcaca
      361 gcctcgcccg cgccgtcttc cacggccagc gcggcgagct acgccagcgc taccgcgagg
      421 gccaggagga ccaactgggc gcgctcggcc tggtcgtcaa cgccatcgcg ctctggaaca
      481 cccgctacac cgaggcggcg ctcacccacc tccgcggcgc cggcgtcgac gtcgcccccg
      541 cggacgtcga gcgcctctcg ccgctcggct ccgcgcacat caacatgctc ggccgctacc
      601 agttcaccct ggccgagccg gtcctccgcg gggagctccg tgagctgcgc gaccccactc
      661 aggaccagga cctcctggcg gtctgatcgc agccgccggg cttaacgtac gttcctgttc
      721 cgttgctact caggggccgg gttcccccgc ctcggcgtgc tccccgcggg tcgacccgtt
      781 cggggcgcgc ggctcgcccg ccgccggacg cgtccgaccg gggacccggc tagcacggcg
      841 tgggcggacg gcctgccccg gtcatggcag cgaccggatg aaggccacga cgtcggcggt
      901 ccgctgatcg tcgagttgct ccgccgcggt cccgggcatc gtcccgagcc cgttccggat
      961 cagtccggcc agcagccgat cgtcgcggta gtaggcgttg agcagcggcc cgtgcagggg
     1021 tgcctccccg cggccgttgt tcccgtggca ggagccgcag ctccgctcgt agggcacccg
     1081 cccgcggccc gcgtcgccgg gccccgcctg gagggtgacc ggcgccggcg ccgggagggg
     1141 ctcgagcgcc accggcgtgg attgggcacg cgccctcccc tcggcggcgt acgcgccgag
     1201 ggcgaggacg gtgcccagca cgagaccgag gaggaccagc caggtcccgg gcttcgccgt
     1261 cgcacccacc ctcggtccct accggtaggt gagcaggtac tgggtgatgt cctcgacctg
     1321 ctcgtgcgtc agctcctgcc ggcccccggg ttccgggatc gtgggcggca tcttggcacc
     1381 cgggttcagc gccttggggt cctcgatgta gcggtggatg aacgccgcgt cccgcagcgc
     1441 gcccacctgc gccagcggcg gcccgtcctt ggtcccctgg cccctctcgc cgtggcagga
     1501 cgcgcaggtg ccctggtaca gggtgcgccc gaccacctgc gcggcgttca gccggacgcc
     1561 gcgctccacc agcgtcgtcg gcggggtcgt cgccgccgcg agcacggtca gaccgccgat
     1621 cgcgagcacg gccgctccgc ccagacccat cgccaccggt cgcctctccg gccgccgccg
     1681 cgggctccgg tcgtagaagg gcaggacgac gagggcgacg accaggacga gcgggaccag
     1741 cgccaccccg acccactcga gcggccccgg gaagtacttg agcagctcga acagccagag
     1801 gaagtaccac tcgggccggg gcacgtagcc ggtgttggtc gggtccgccc gcggctccag
     1861 gggggcgccg gccagcacgg ccaggccgac cagcaccacg aacacggcca ggctgacgag
     1921 cgcgtcctgg gcgatcagat cgggccagaa ggggtgcccg cgctccttga agcgggcgta
     1981 ccgcgcgtcc tcctcgaccg gcagctcggc cgaccggcgc tcccacggcc agggcgggac
     2041 cgagacgccg aggtagacga ccaggtagag gtgcgccgcg acggtgacga tgagcagccc
     2101 cggcagcacc aggaggtgca gcccgtagaa gcgggtcagc gtcaacgcac cgacctccgg
     2161 tccaccgcgc acgatccgca gcagggcgtc gccgacgagt gggaccgtgc cggccatgct
     2221 cgtgccgacg gtggtcgccc agtaggcctt ctcgtcccag ggtagcaggt agccggtgaa
     2281 gcccaggccg atcgtggcca gcaggagcac cacgccgacc agccaggtca gctcccgggg
     2341 ccgcttgtag gcaccgaaga cgaaggcggt gaggaggtgc
//
LOCUS       DASPWO010000562         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_249901, whole genome shotgun sequence.
ACCESSION   DASPWO010000562 DASPWO010000000
VERSION     DASPWO010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249901"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..690)
                     /locus_tag="VEO00_12880"
     CDS             complement(262..690)
                     /locus_tag="VEO00_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYU58923.1"
                     /translation="MKVVDANVLLYSVNEDSPLHERARSWLDSALGGSEAVGFSWVAL
                     LAFIRLSTRADIFLSPLTPAEAMSVVESWLAAPAAVVLHPTPRHLSVLAGLLAPFGTA
                     ANLVNDAHLAALAVEHNADVMSFDRDFGRFPGVRWQEPPA"
     gene            complement(721..978)
                     /locus_tag="VEO00_12885"
     CDS             complement(721..978)
                     /locus_tag="VEO00_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HYU58924.1"
                     /translation="MRTTITLEPDVEVLVRRFMRERGLSFKQAVNLAIREGLSPSSTE
                     RSFRTPTFRMGSAPGTSLDKALRLAAELEDEELARKLATRK"
     gene            complement(994..1371)
                     /locus_tag="VEO00_12890"
     CDS             complement(994..1371)
                     /locus_tag="VEO00_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58925.1"
                     /translation="MAVLKTLQAEGSGRWKIRRLQEVLHWLEPASTTELAAELRAAGV
                     LAYDPVSGIYRLTTEAHVVTAILDPLTVPGLDPRRLATAFYGRYGVRPLPGSPGRLVI
                     PMSTVAEALELRERGVMYLHRRF"
     gene            complement(1483..1878)
                     /locus_tag="VEO00_12895"
     CDS             complement(1483..1878)
                     /locus_tag="VEO00_12895"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HYU58926.1"
                     /translation="MKFGFKELPGTREDYLRPVVPVTVEGIARAPQLCLLDTGALHNR
                     FAAWVAEAAGIDLGDSVEERLAVGGFVSAARSARVQLTLGDVTWEAPVWFCDPWPLAF
                     HLLGQEGFFRWFEVRLRAARYEIEITPEG"
     gene            complement(1875..2348)
                     /locus_tag="VEO00_12900"
     CDS             complement(1875..2348)
                     /locus_tag="VEO00_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58927.1"
                     /translation="MSQVQATIARRDFVLDSSDVERRLRDVLPDPLDDHYVVVAGRRF
                     PPKQVISLMTDLDRADFNTHQARRILSRLGFTVGRRSRESTPPSRRPGPHGGREADLL
                     RPFRGRWVAQRGLEVLVAADTPQPVLAWLERHNQQADAMFRVPDDEAEAAGAAPA"
     gene            complement(2391..3344)
                     /locus_tag="VEO00_12905"
     CDS             complement(2391..3344)
                     /locus_tag="VEO00_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HYU58928.1"
                     /translation="MGGRSEDSLATVLLAGRLLDGDARAFSPKEFWHLVGRVPEPGGL
                     LGADAAAIGATVGTPPSEGERIAGLLEGATRLAFELERLELSGIHVLTPFDERYPASF
                     PDRLGTAAPPVLHVAGDASLMARPGIGVAGSRDASMGALDVAAAAAGVAVEHGWPVVS
                     GAARGVDQAALAEAFRRDGTAVGVPADALDRRLRDPDTRRAITSGRVCLVTPYRPSAG
                     FSVANAMGRNKLIYALSRVTLVVATDLESGGTWAGATEALSRKIAPVAVWTGPGGGPG
                     NPALVPLGAHEIHDVDDLAADWTQPDRQSEGAPAQQLGLGI"
     gene            complement(3344..>3630)
                     /locus_tag="VEO00_12910"
     CDS             complement(3344..>3630)
                     /locus_tag="VEO00_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HYU58929.1"
                     /translation="AFARSVAVELGIEFRPAAVKVRDNRPQKEMENSAQQAANVLDAF
                     EVRGKVPDGPVLLIDDIIDSRWTINVIGTLLREAGSGPVFPFVLAEAVST"
BASE COUNT          541 a         1326 c         1251 g          512 t
ORIGIN      
        1 caccgagatc gcagttcacc gacagggtgg ccgcgtgcgc gccgggcgaa gcgaccccga
       61 tgaccgccgc caccatgagg ccgaccgtcg caccgccgat cactcgcctc ggcgtgatcc
      121 gtgagcgtcc ccttcgtcgc atcgagcctc ctccgtcaga ccaggcgaac cagtccacgg
      181 cgggctcgtg gggtggtggc ttcgtaggct agatcgtgac gtggtcgccc gcgagacgaa
      241 ctgctcggtc ccgcatgtcg atcaggcggg cggttcctgc caccgaacac cggggaaacg
      301 gccgaagtcc cggtcgaacg acatcacgtc ggcgttgtgc tcgaccgcga gggcggcgag
      361 gtgggcgtcg ttcacgaggt tcgcggcggt gccgaacggg gccagcaggc cggcgagaac
      421 ggagaggtgc cgcggcgtgg ggtgcaggac gaccgccgcg ggcgcggcca gccacgattc
      481 gacgacgctc atcgcctccg cgggcgtcag cgggctgagg aagatgtccg cacgggtgct
      541 cagccggatg aaggcgagga gcgccaccca ggagaagccc accgcctcgg agcccccgag
      601 ggcagagtcg agccacgacc gcgctcgttc gtgcaggggc gagtcctcgt tcaccgagta
      661 caggagcacg ttcgcgtcga cgaccttcat gcgaaccggt cgccccgccc cgggctcacg
      721 tcacttcctg gtcgcgagct tgcgggcgag ctcctcgtcc tcgagctcgg cggccagccg
      781 gagggccttg tccagggagg tcccgggggc ggaccccatg cggaaggtcg gcgtgcggaa
      841 cgaccgttcg gtggagctcg gggagagtcc ctcccggatg gccaggttca ccgcctgctt
      901 gaacgacaac ccccgctcgc gcatgaatcg tcgaaccagc acctcgacat cgggctcgag
      961 cgtgatcgtc gtccgcacct gtgcatcata gcatcagaat cggcgatgca gatacatcac
     1021 cccgcgctct ctcagctcca gagcttccgc caccgtcgac atcggaatga ccaggcgtcc
     1081 gggagagccg ggcagcggcc ggacgccgta cctcccgtag aacgctgtgg cgaggcggcg
     1141 cgggtcgagg ccgggcacgg tgagggggtc gaggatggcg gtgaccacgt gggcctccgt
     1201 ggtcaggcgg tagatccccg agacggggtc gtaggccagg accccggcgg cgcgaagctc
     1261 ggcggccagc tcagtggtgg aggccggttc gagccagtgg aggacctcct ggaggcgccg
     1321 gatcttccat cgccccgagc cctccgcctg gagggtcttg agcacggcca ccttgctgct
     1381 gctcctccac cgggcttccc tcgattccgg gcgagtatag ggggaccctc cgacacgtcc
     1441 cgtgaggtct cccgggttcc ttcgtggcct ggcgccgggc ttctatccct cgggcgtgat
     1501 ctcgatctcg tagcgggcgg cccggagtcg aacctcgaac caccggaaga agccctcctg
     1561 gccgagcagg tggaacgcga gtggccatgg atcgcagaac cagactgggg cctcccaggt
     1621 cacgtcgccg agcgtcagct gcacccgggc cgatcgagcg gccgacacga atcccccgac
     1681 ggcgagccgc tcctcgaccg agtcgccgag gtcgatgccg gctgcctcgg cgacccaggc
     1741 ggcgaacctg ttgtgcagcg cgccggtgtc gagcagacag agctgcggag ctcgcgcgat
     1801 gccctcgacc gtcacgggga cgacgggacg gaggtagtcc tcgcgcgttc ccgggagctc
     1861 cttgaacccg aacttcatgc gggcgccgcg ccggcggcct ccgcctcgtc gtcgggaacc
     1921 cggaacatgg cgtcggcctg ctggttgtga cgctcgagcc atgcgaggac gggttgtggc
     1981 gtgtccgccg ctacgagaac ctcgagcccg cgttgagcga cccatcgccc ccggaacggc
     2041 cggagcaggt ccgcttcccg ccccccgtgg ggccccggcc ggcgcgaagg gggtgtcgac
     2101 tcgcggctgc gccgccccac cgtgaagccc aggcggctca ggatccggcg cgcctgatgc
     2161 gtgttgaaat cggcccggtc gaggtccgtc atcaggctga tcacctgctt cggaggaaac
     2221 ctcctgccgg ccaccacgac gtagtggtcg tccagcggat ccggcagcac gtcccggagc
     2281 cgccgttcga cgtccgacga atcgagaacg aagtcccgcc gggcgatcgt ggcctgtacc
     2341 tgactcatac ttactgtata gcacttacca gtccgtccca ccagtgagcg tcagatcccg
     2401 aggccgagct gttgcgccgg ggcgccctcg gactggcgat ccggctgggt ccaatcggcg
     2461 gcgagatcat cgacgtcgtg gatctcgtgc gccccgaggg gcacgagcgc cgggttcccg
     2521 gggccgccgc ctggtcctgt ccacaccgcg accggggcga tcttccgcga caaggcctcg
     2581 gtggcgcccg cccaggtgcc gcccgattcc aggtcggtgg ccacgaccag cgtgaccctc
     2641 gacagcgcgt agatcagctt gttgcggccc atggcgttcg ccacgctgaa gccggcggac
     2701 ggccggtacg gcgtgaccag gcacacccgg cccgaggtga tggcgcgtcg cgtgtccggg
     2761 tccctcagcc ggcggtcgag cgcgtcggcc gggacgccga cggctgtgcc gtcccgtcgg
     2821 aaggcctccg ccagcgcggc ctggtccacg ccacgggcgg cgcccgagac caccggccac
     2881 ccgtgctcca ctgcgacccc ggcggccgcc gcggcgacgt ccaacgcgcc catggaagcg
     2941 tcccgggaac cggccacccc gatccccggg cgagccatca gcgaggcgtc gccggccaca
     3001 tggaggaccg ggggggccgc ggtgcccagg cggtctggga acgacgccgg gtaccgttcg
     3061 tcgaacggcg tcaggacgtg gatcccgctc agctccagcc gctccagctc gaacgccagc
     3121 ctcgtggccc cctcgagcag tccggcgatc cgctcgccct ccgatggcgg cgttcccacc
     3181 gtcgccccga tcgccgccgc gtcggcaccg agcagccctc ccggctccgg cacgcggccg
     3241 accaggtgcc agaactcctt cggcgagaac gcccgcgcat ccccgtccag cagcctcccc
     3301 gccaggagca cggtggccag cgagtcctcc gagcgcccgc ccatcaggtg ctcaccgcct
     3361 ccgccagcac gaacgggaac accggcccgc tccccgcctc acgcagaagc gtcccgatca
     3421 cgttgatcgt ccatctcgag tcgatgatgt catcgatgag cagcaccggg ccatccggca
     3481 cctttccccg cacctcgaac gcgtccagga cgttggccgc ctgctgtgca ctgttctcca
     3541 tctccttctg ggggcggttg tcgcggacct tcaccgcggc gggcctgaac tcgatgccga
     3601 gctcgactgc caccgaccgg gcgaacgccg
//
LOCUS       DASPWO010000563         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_420423, whole genome shotgun sequence.
ACCESSION   DASPWO010000563 DASPWO010000000
VERSION     DASPWO010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420423"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1035
                     /locus_tag="VEO00_12915"
     CDS             247..1035
                     /locus_tag="VEO00_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYU58930.1"
                     /translation="MSLVVSRPGRVWRLVLSRPEQRNAVSTSMMEELSAGLADAAASP
                     EARAVVLEGAGRHFCAGADVAELLEATGGPGAVEYGRALEAVLVAVGEHPLPVIAGVQ
                     GAALGAGCQLAVACDLAIAAEDARFGIPSSRLGVLVNYENVERLTLAIGPKRAAELLL
                     TGRELPGTEAAAWGLVNAAVPEAGLDDAVDALAESVAAGAPFSVRGSKLGIREVLRAQ
                     AAPRRAEGSRGAFDMLAAEALASEDLREGIAAFREQRAPEFRGK"
     gene            1038..1718
                     /gene="nth"
                     /locus_tag="VEO00_12920"
     CDS             1038..1718
                     /gene="nth"
                     /locus_tag="VEO00_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYU58931.1"
                     /translation="MVREAGDTEGKRLTRNDPVEERAPEIIRRLKDAYPEAKVALKFS
                     NALEMLVATILSAQCTDEKVNEVTATLFQKYPHAQAYLAAPEEELRRDIYSTGFYNQK
                     AKAIRSACRMIVEEFGGRVPDTMEDLVKLPGVARKTANIVLGNAYGTVVGIAVDTHVK
                     RLAHRLGFSAQKDPDKVERDLMRLIPREDWFPFTYVLIDHGRAVCVAKTPRCEVCVVN
                     DLCPSSRV"
     gene            complement(1704..2312)
                     /locus_tag="VEO00_12925"
     CDS             complement(1704..2312)
                     /locus_tag="VEO00_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HYU58932.1"
                     /translation="MGSIAAAIRPRERPAGILTDAGLIAGGSLAVAALAQVSIHLRFV
                     PITGQTLGVLLVAASLGMWRGGAAIALYLAEGAAGLPFYAEARGGLDYLVLRDPLHVS
                     GGYLWGFLLAAVVVGFLADRGWMRRFGSAVGAMFLGNVAIYLVGLPWLAAAIGTSVED
                     ALPFGLHPFVIGDTIKLLLAAALLPAASRMVGSDRARPSDAA"
     gene            2407..>3141
                     /locus_tag="VEO00_12930"
     CDS             2407..>3141
                     /locus_tag="VEO00_12930"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HYU58933.1"
                     /translation="MSLFHELIGSRTRLTPATTDHLARLVGEWQLLADLAFADLLLLA
                     PDRGGGFVVLAQMRPYTGQTVYQDDQVGRTLSAAEAPQAARAIDQARIVREGDPVERG
                     GVAVRVEAIPVRFQGEVVAVVSQEQNLSAPRTPSRLELTYLRCAGDLDRMIMEGTFPH
                     QAAEPDPENSPRVGDGLIRLDARGTVEYASPNAVSGYRRLGVTVNLIDRPFAEVDPAP
                     DEVVGTLLSRRPAESEVERRGAVVLRR"
BASE COUNT          462 a         1103 c         1180 g          396 t
ORIGIN      
        1 cgcgtcctcc gggaccgact gcgccagatc gacggacgcg atgcatccac tcacctccgg
       61 cacagcgacg aggtgagaag agaggagggg cgcctcgtgc gcgaccgcct cgaacgcgcc
      121 atatgggctc tgacggcgcg caccctaccg agaggacaat ccgcggtcaa ctccgtaccg
      181 gcccccaggt cttgcccggg agtcgccaac tctcgcgacc gttctccggc ggctagcatc
      241 ggacgcgtga gcctcgtcgt ctcgcggccg ggcagggtct ggcggctggt gctgtcgcgg
      301 cccgagcagc gcaacgcggt ttccacgtcg atgatggagg agctgtcggc gggcctcgcc
      361 gatgctgcgg cgtcgcccga ggcgcgagcg gtcgtgctgg agggcgccgg gcggcacttc
      421 tgcgccggtg cggacgttgc ggagctgctc gaggcgaccg gcgggcccgg cgcggtggag
      481 tacggccggg cgctcgaggc ggtgctcgtg gcggtcggcg agcatccgct gccggtcatc
      541 gccggggtgc agggcgccgc cctcggggcc gggtgccagc tggcggtggc ctgcgacctg
      601 gcgatcgccg cagaggacgc gcggttcggc atcccgtcga gccggctcgg cgtgctggtc
      661 aactacgaga acgtcgagcg gctgacgctc gcgatcgggc cgaagcgcgc cgcggagctg
      721 ctgctcaccg ggcgtgagct gccggggacc gaggccgccg cgtggggcct ggtcaacgcg
      781 gcggtcccgg aggcggggct cgacgacgcg gtggacgcgc tggcggagtc ggttgcggcg
      841 ggagcaccct tctcggtgcg cgggtccaag ctcggcatcc gcgaggtcct ccgcgcgcag
      901 gccgccccgc gccgggcgga agggtcgcgg ggggcgttcg acatgctcgc cgccgaggcg
      961 ctcgccagcg aggacctgcg cgagggcatc gccgccttcc gcgagcagcg cgcgccggag
     1021 ttccggggga agtgagcgtg gtgcgcgaag cgggcgacac ggaagggaag cggctcaccc
     1081 ggaacgaccc ggtcgaggag cgggcgccgg agatcatccg ccggctgaag gacgcctacc
     1141 cggaggcgaa ggtggcgctg aagttctcga acgcgctcga gatgctcgtc gccacgatac
     1201 tgtcggcgca gtgcaccgac gagaaggtga acgaggtcac cgccacgctg ttccagaagt
     1261 acccgcacgc gcaggcctac ctggcggcgc cggaggagga gctgcggcgc gacatctact
     1321 cgaccgggtt ctacaaccag aaggcgaagg ccatccgcag cgcctgccgg atgatcgtcg
     1381 aggagttcgg cgggcgcgtc cccgacacga tggaggacct cgtcaagctc cccggcgtgg
     1441 cgcgcaagac cgccaacatc gtgctcggca acgcctacgg caccgtcgtg ggcatcgccg
     1501 tcgacaccca cgtcaagcgc ctggcgcacc ggctcgggtt ctcggcgcag aaggacccgg
     1561 acaaggtgga acgggacctg atgcgcctca tcccgcgcga ggactggttc ccgttcacct
     1621 acgtcctgat cgaccacggc cgcgcggtgt gcgtggcgaa gaccccgagg tgcgaggtct
     1681 gcgtggtgaa cgacctctgc ccgtcaagcc gcgtctgagg gccgggcgcg atccgagccg
     1741 accatccggg aggcggccgg cagcagcgcg gcggcgagca gcagcttgat cgtgtccccg
     1801 atgacgaacg ggtggagccc gaacggcagg gcgtcctcca ccgaggtccc gatcgccgcg
     1861 gcgagccacg gcaggccgac caggtagatc gccacgttgc ccaggaacat ggcccccacc
     1921 gcgctgccga accggcgcat ccagccgcgg tcagcgagga atccgaccac cacggccgcc
     1981 agcaggaacc cccacaggta cccgccggaa acgtgcagcg ggtcgcggag cacgaggtag
     2041 tccaggcctc ccctcgcctc ggcgtagaag ggcagcccgg ccgcgccctc cgccagatag
     2101 agcgcgatgg cggcgccacc gcgccacatc ccgagggacg ccgcgacgag cagcaccccg
     2161 agcgtctgtc cggtgatcgg cacgaagcgg aggtggatcg agacctgggc cagcgccgcc
     2221 accgccaggc tcccgccggc gatcagcccg gcgtcggtca ggatcccggc cggtcgctca
     2281 cggggccgga tggctgcggc gatgctgccc acggttcctc ctcagttcgg tcgaggcatg
     2341 ccttccatcg agggatgtct acgggagcgg accctccggc gcaactccgg gcgctaccat
     2401 cggcatatgt cgctgttcca cgagctgatc gggtcgcgca cgcgcctgac gccggcgacg
     2461 accgaccacc tggcccgatt ggtcggggag tggcagctgc tggccgacct ggcgttcgcc
     2521 gacctcctgc tgctcgcccc cgaccgggga ggtggcttcg tcgtcctggc gcagatgcgg
     2581 ccctacaccg gccagacggt ctaccaggat gaccaggtcg ggcggaccct gtcggcggcg
     2641 gaggcaccgc aggccgcgcg ggcgatcgac caggcacgga tcgtgcgcga gggcgatccc
     2701 gtggagcgcg gaggggtcgc ggttcgcgtg gaggccatcc cggtgcggtt ccagggtgag
     2761 gtcgtcgccg tggtcagcca ggagcagaac ctgtcggcgc cgcggacgcc gagccgcctg
     2821 gagctgacgt acctgcgctg cgccggcgac ctcgaccgga tgatcatgga gggcacattc
     2881 ccccatcaag ccgccgagcc cgacccggag aactcgcccc gcgtcggcga cggcctgatc
     2941 cgcctcgacg cacgcggcac cgtggagtac gccagcccga acgcggtctc ggggtaccgc
     3001 cggctcgggg tgactgtgaa cctgatcgac cggccgttcg ccgaggtcga tccggccccg
     3061 gacgaggtcg tcgggacgct gctctcccgg cgcccggcgg agagcgaggt cgagcgccgc
     3121 ggcgcggtcg tgctgcgccg g
//
LOCUS       DASPWO010000564         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1180324, whole genome shotgun sequence.
ACCESSION   DASPWO010000564 DASPWO010000000
VERSION     DASPWO010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180324"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VEO00_12935"
     CDS             complement(<1..601)
                     /locus_tag="VEO00_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58934.1"
                     /translation="MTRGGRRAWALAAGAILALVVLAGPAAATSGAQPASPRRQVFLF
                     LVDRASFEEVLAVPELAALARAGGAGLANFLIGGDDVSLEPYVMLGSGVRAAAPFGRI
                     APAGELVSGSDLAALEAENDRAVPGLLGEALADGGLAVGVLGNADDRAGVNAPGLLAG
                     MDEDGRIPDARVAGLTVSAPGRLGGLRTDGAALIAESERM"
     gene            complement(598..1869)
                     /gene="murA"
                     /locus_tag="VEO00_12940"
     CDS             complement(598..1869)
                     /gene="murA"
                     /locus_tag="VEO00_12940"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HYU58935.1"
                     /translation="MDRLLVTGGARLSGSVPISGAKNSALKLMAAALLAEGTTTIHRV
                     PDIADCRTMVDVLEVLGAGVSRDGGTVRIDASGPLGLETPYDLVRRMRASIIVLGPLV
                     ARFGRARVAMPGGCNIGSRAIDLHVKGLERMGAQFTHEHGYLEASASRLEGAVVSLDF
                     PSVGATENVMMAAVAARGTTVIENAAREPEIQDLADLLVAMGGRIFGAGTTTVEIEGV
                     DGFRPVEHTVIPDRIEAGTYALAAAATGGDVLLEGARADHLDLFLAKLEEMGVRTAAE
                     ERGLRVTAPERVEAADVVTLPYPGFATDLQPQAMAVLVAARGTSIVTENVFESRFMFV
                     DELNRMGADIRTEGHHAVIRGVERLSAAPVRALDIRAGAAMIIAALGADGVTEIADLH
                     HVDRGYEAFEDKLHALGAQVGRQPALSPAVP"
     gene            2005..2253
                     /locus_tag="VEO00_12945"
     CDS             2005..2253
                     /locus_tag="VEO00_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58936.1"
                     /translation="MSPEDGGRLEVEWEVDSHVAGQRWNMAIKRAGVLLKSGRRTTDA
                     CGDAELRVVVSNAAGTDAFRGKAANLATGETCKGTISF"
     gene            2331..2942
                     /locus_tag="VEO00_12950"
     CDS             2331..2942
                     /locus_tag="VEO00_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006620210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYU58937.1"
                     /translation="MALTDRFRDALALAFDLHRGQVRKGTRIPYVSHLLGVCDLVLFD
                     GGDEHEAIAALLHDAAEDQGGREVLERIRERFGKRVAGIVEACSDALVPKGAEKPPWR
                     TRKEAYLARLPEHPEAWRVSLADKLNNARAVLADLRRADDREQVWNRFNGKKDGTLWY
                     YRAMADFFGEGRRGWMAEELDRTVGELEALAEERAARTITGNG"
BASE COUNT          427 a         1129 c         1115 g          377 t
ORIGIN      
        1 ccatccgttc cgactcggcg atcagcgccg cgccgtcggt tcgcaggcca ccgagacggc
       61 cgggcgcgga gaccgtcagc cccgccacgc gtgcgtccgg gatccgtccg tcctcgtcca
      121 tccccgccag caggccgggc gcgttcacgc cggcccggtc gtcggcgttc ccgagcaccc
      181 cgacggcgag ccccccgtcg gcgagggcct cgccgagcag gcccggaacg gcccggtcgt
      241 tctccgcctc cagcgcggcc aggtcggacc cggagaccag ctcgcccgcc ggggcgatcc
      301 gcccgaacgg ggccgccgcc cggacacccg agccgagcat gacgtacggc tcgagggaga
      361 cgtcgtcgcc accgatcagg aagttcgcca gcccggcgcc gccggcgcgg gccagcgccg
      421 cgagctccgg gaccgccagg acctcctcga acgatgcccg atccaccagg aacaggaaca
      481 cctgccgtcg cggggaggcc ggctgcgcgc cggacgtggc ggccgccggg ccggcgagca
      541 ccacgagcgc gaggatggcg ccggcggcga gggcccacgc ccgccggccg ccccgcgtca
      601 cgggacggcg ggcgacagcg ccggctggcg gcccacctgc gcgccgagcg cgtggagctt
      661 gtcctcgaag gcctcgtacc cgcggtcgac gtggtgcaga tcggcgatct cggtgacgcc
      721 gtccgcgccg agcgccgcga tgatcatcgc cgccccggcc cggatgtcga gcgcgcgcac
      781 cggcgccgcc gacaaccgct ccaccccgcg gatcaccgcg tggtgcccct cggtgcggat
      841 gtccgcgccc atccggttca gctcgtcgac gaacatgaag cggctctcga agacgttctc
      901 ggtgacgatg ctcgttccgc gggcggcgac gaggactgcc atcgcctgag gctgcaggtc
      961 ggtggcgaac cccggatacg ggagcgtgac gacgtccgcc gcctcgaccc gctcgggcgc
     1021 cgtcacccgc aggccgcgtt cctcggcggc cgtgcgcacg cccatctcct cgagcttcgc
     1081 caggaacagg tccaggtggt cggcgcgggc cccctccagc aggacgtcgc ccccggtggc
     1141 ggccgcggcg agggcgtagg tgccggcctc gatccggtcg gggatcaccg tgtgctcgac
     1201 gggccggaag ccgtccacgc cctcgatctc caccgtcgtc gtcccggccc cgaagattcg
     1261 ccctcccatc gcgacgagca ggtcggcgag gtcctggatc tcgggttccc tggccgcgtt
     1321 ctcgatcacc gtggtgcccc gggcggcgac cgcggccatc atcacgttct cggtcgcgcc
     1381 gacgctcgga aaatcgagcg agacgacggc gccctccagc cgggacgcgg aggcctccag
     1441 gtagccgtgc tcgtgggtga actgcgcgcc catccgctcc aggcccttga cgtggaggtc
     1501 gatggctcgc gagccgatgt tgcagccgcc cggcatggcc acccgggccc gcccgaacct
     1561 cgccacgagc gggccgagca cgatgatcga agcgcgcatc cggcgaacga ggtcgtaggg
     1621 cgtctccagg cccagcgggc cggaggcgtc gatgcgcacg gtcccgccgt cgcgcgagac
     1681 gccggccccg aggacctcga gcacgtccac catcgtccgg cagtcggcga tgtcgggaac
     1741 acggtggatc gtggtggtgc cctcggccag cagcgccgcg gccatcagct tcagcgccga
     1801 gttcttggct cccgagatcg gaacggatcc cgaaaggcgc gcgccgccgg taacgaggag
     1861 gcggtccatg gcggcgattc tacgggccta tcactccctt aacgctcggt tcgccgagcc
     1921 atggacggac cgttgccggc gatcgcgcgc gacagcgttc ggacgcaggg cccgtgctcg
     1981 gcccgaagcg cgtggaagtt cagggtgagc ccggaggacg gcggtcggct cgaggtcgag
     2041 tgggaggtgg actcccacgt cgccggccag cgctggaaca tggccatcaa gcgggcgggc
     2101 gtgctgctga agtcgggccg gcgcacgacc gacgcgtgcg gcgacgctga gctgcgggtg
     2161 gtggtctcga acgctgccgg gacggacgcc ttccgcggga aggcggcgaa cctggccacc
     2221 ggagagacct gcaagggcac gatctccttc tgaccgacga ggatcggcga acgggcggcc
     2281 tccgggcccg cgcatcgatc tccgatcccc accgccggta ggctccgggg gtggcgctga
     2341 ccgatcggtt ccgcgacgcg ctggccctcg ccttcgacct gcaccgcggt caggtccgca
     2401 agggcacgcg gatcccctat gtctcgcacc tcctcggggt gtgcgacctg gtcctgttcg
     2461 acggcggcga cgagcacgag gccatcgccg cgctcctgca cgacgccgca gaggaccagg
     2521 gcggccgcga ggtcctcgag cggatccggg aacggttcgg gaagcgcgtc gcgggcatcg
     2581 tcgaggcatg ctccgacgcg ctggtcccca agggcgccga gaagccaccg tggcgcaccc
     2641 gcaaggaggc ctacctcgcc cgtctcccgg agcacccgga agcctggcgg gtgtcgctgg
     2701 ccgacaagct gaacaatgcc cgcgccgtgc tggcggacct tcgccgggcc gacgaccggg
     2761 agcaggtctg gaaccgcttc aacggcaaga aggacgggac gctctggtac taccgggcga
     2821 tggccgactt cttcggggaa ggccggcggg gctggatggc ggaggagctc gaccgaacgg
     2881 tcggcgagct cgaagcgctg gccgaggagc gggcagctcg aacgatcacc gggaacgggt
     2941 aacggctagc cgggctcgct cagcgcgagc cgggcctcgg cgcgggccag cgccgctcgg
     3001 gccgcctcgt catctcggtc ggcctcgagg cgctcccggg ccgcggca
//
LOCUS       DASPWO010000565         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_420809, whole genome shotgun sequence.
ACCESSION   DASPWO010000565 DASPWO010000000
VERSION     DASPWO010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420809"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VEO00_12955"
     CDS             <1..718
                     /locus_tag="VEO00_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYU58938.1"
                     /translation="TAPREVVALGRTGWFAGGLAGAALFAGFAFQTIGLQYTTASKAG
                     FVTGLYVVFTPLLAAAVLRRRPSAGVAAGALIAVAGLALLSFSGGAIAVRYGDALVVA
                     CAVAFALQFVVLGRYAPGLPSGGLAAAQLWVCGILATALSLAIEDPVAPRTAAVWAAL
                     GITAVLASALAFVVQAVAQRFISPSRTALILVMETPFAALFGFLLLDERFAGWQWAGA
                     ALILAGMLAAELAPARPDEA"
     gene            complement(728..>2259)
                     /locus_tag="VEO00_12960"
     CDS             complement(728..>2259)
                     /locus_tag="VEO00_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYU58939.1"
                     /translation="GHSTVGEVEASGWLLGPFPAAGLWRPVLVNALAGADWGAVAGRA
                     ADLATVVFVSTLSLLLNASSIELVTNRDVDLDRELRAAGVGNLVAGAGGGIGGFHALS
                     LTTLTHRSSAASRWTGMVAAAVVTGTLLFGAALLGFVPRLVMGGVVMFLGLGFLFEWV
                     VDARRKLPLPEYLIVLFILFVIAATGFMQGVALGLVIALVLFAVAYSRTQQIRDSVSG
                     AVFHSNVDRGPHERQVLARAGAHIHLLHLQGFLFFGTANEVLQRIRERADAPEPARLE
                     FLILDFERVTGVDASAVLAFERAARMAAQRGFVVIFSEVPPPVQRLLEHAGLTGGDVV
                     RYAEDADRAVEWCEERILERAGVGPAADEMPLADRIRARATDEGVDPARIVRHLERME
                     LEGGQVLIRQGDTDQDAYFLERGRLTVLLRREDGEEMRLRTLRPGTVVGELAVYLGGE
                     RTASVVADGRAVVYRLGVEARRRMAEQDPAAAAALHAILATILAERLRATIDTVEALM
                     D"
BASE COUNT          292 a          883 c          775 g          309 t
ORIGIN      
        1 caccgcccct cgcgaggtgg tcgcgctggg gcgcaccggc tggttcgccg gcgggctggc
       61 cggcgcggcg ctcttcgctg ggttcgcgtt ccagacgatc gggctgcagt acacgaccgc
      121 ctcgaaggcc ggcttcgtca ccggcctgta cgtggtgttc acccccctgc tggccgcggc
      181 cgtgctgcgc cgccgcccgt ccgcgggggt cgcggccggc gcgctcatcg cggtcgccgg
      241 cctcgcgctg ctgtcgttct cgggaggcgc gatcgccgtc cgctacggcg acgccctcgt
      301 cgtcgcctgc gcggtcgcct tcgccctcca gttcgtcgtc ctcggccgct acgcgcccgg
      361 gctgccgtcc ggcgggctgg ccgccgctca gctgtgggtg tgtgggatcc tcgccaccgc
      421 gctcagcctg gcgatcgagg accccgtcgc gccgcgcacg gcggcggtct gggccgcgct
      481 cgggatcacc gcggtgctcg cctccgccct ggccttcgtc gtccaggccg tggcccagcg
      541 cttcatctcg ccaagccgaa ccgcgttgat cctggtgatg gagacgccgt tcgccgcgct
      601 gttcgggttc ctcctgctcg acgagcgctt cgccggctgg cagtgggccg gcgccgccct
      661 gatcctcgcc gggatgctcg ccgcggagct ggctccggcg cgccccgacg aggcctgatc
      721 cccggcctca gtccatcagc gcctcgaccg tgtcgatcgt cgcgcggagg cgctcggcga
      781 ggatcgttgc caggatggcg tgcagggctg ccgcggcggc ggggtcctgc tcggccatcc
      841 gtcgccgggc ctccacgccc agccggtaga cgacggcccg gccgtcggcg accacgctcg
      901 ccgtgcgctc cccgcccagg tacacggcca gctcgccgac caccgttccc ggcctgagcg
      961 tgcgcaggcg catctcctcg ccgtcctcgc gccggagcag gacggtcagc cgcccgcgct
     1021 ccaggaaata ggcgtcctga tccgtgtcgc cctggcggat caggacctgg ccgccttcga
     1081 gctccatccg ttccagatgg cgaacgatcc gggcggggtc caccccctcg tcggtggcgc
     1141 gggcgcggat gcgatccgcc agcggcatct cgtccgccgc ggggccgacc ccggcccgct
     1201 ccaggatgcg ctcctcacac cattcgaccg ctcggtcggc gtcctccgcg tagcgaacca
     1261 cgtccccccc ggtcaggccg gcgtgctcca gcagccgctg aacgggcggc ggaacctcgg
     1321 agaagatgac gacgaaccct cgctgcgctg ccatcctcgc cgcccgctcg aacgccagca
     1381 ccgccgaagc gtccacgccg gtcacacgct cgaagtcgag gatcaggaac tccaggcggg
     1441 ccggctccgg tgcgtccgcc cgctcccgga tccgctgcag cacctcgttc gccgtaccga
     1501 agaacaggaa cccctgcagg tgaaggaggt ggatgtgcgc gcccgcccgg gcgagcacct
     1561 ggcgctcgtg cgggccgcga tccacgttgc tgtggaacac ggcgccggac accgagtcgc
     1621 gaatctgctg cgtccggctg tacgccacgg cgaacagtac gagcgcgatc acgaggccca
     1681 acgccacgcc ctgcatgaac ccggtggcgg cgatcacgaa caggatgaac agcacgatca
     1741 ggtattccgg gagcggcagc ttcctccggg cgtcgaccac ccactcgaag aggaagccga
     1801 ggccgaggaa catcactacg ccgcccatca cgaggcgcgg cacgaagccc agcagcgccg
     1861 ctccgaacag cagcgtcccg gtcacgacgg cggccgccac catcccggtc catcggctgg
     1921 cggcgctcga ccgatgcgtg agcgtggtca ggctcagcgc gtggaagccg ccgatgccgc
     1981 cgccggcgcc ggcgacgagg ttccccacgc ccgccgctcg gagctcgcgg tcgaggtcga
     2041 cgtcgcggtt cgtgaccagc tcgatcgagc tcgcgttcag cagcaacgac agcgtggaga
     2101 cgaacaccac ggtggcgagg tccgcggcgc gcccggccac ggcgccccag tccgcgccgg
     2161 cgagggcgtt caccagcacc ggccgccaga gcccggcggc ggggaacgga ccgagcagcc
     2221 atccactcgc ctccacctcg ccgacggtcg aatggccaa
//
LOCUS       DASPWO010000566         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_420850, whole genome shotgun sequence.
ACCESSION   DASPWO010000566 DASPWO010000000
VERSION     DASPWO010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420850"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..938)
                     /locus_tag="VEO00_12965"
     CDS             complement(96..938)
                     /locus_tag="VEO00_12965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HYU58940.1"
                     /translation="MTFDLVRRWLPGTGRGSLADPGLFPPGSVAGRVNAETALLLGGG
                     RALLLQLAHPMVAAGVADHSDFRRDAFGRLANTLDLTLTVAFGDEDQRRAAASRVTET
                     HRLVTGSRGPRAYRALDPELLLWVHATLVDGAIVTYERFVGSIGGAARGRYHEEMKRQ
                     AVAFGIPRDRLPVTLGDFRRYVDRMVATLHVSDEARLLSRGVLDPRTPTALRPLAGLM
                     RFVTAGLLPPRIRAAYDLAWDPRRERALGALAAAIRGGVVPVLPDAWRRWPHARDADR
                     RMVG"
     gene            complement(983..1324)
                     /locus_tag="VEO00_12970"
     CDS             complement(983..1324)
                     /locus_tag="VEO00_12970"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HYU58941.1"
                     /translation="MATITDYSFGHLVVDGRPETRDVIVLPGRIVRNWRRRDGHVLVF
                     QDLEGVLEELPERLVVGMGASSQMRPDPATLERLRERGVDVECLPTDRAVIRFGELDP
                     ARTAAALHLTC"
     gene            1503..>2493
                     /locus_tag="VEO00_12975"
     CDS             1503..>2493
                     /locus_tag="VEO00_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYU58942.1"
                     /translation="MRRRIVRLAAVLAVIMSVVGSVGASASPKRGLRLAPESVSLASL
                     IEAPRSLTGKLAETDPSLLGKTSSELVNVMVKLDYDPIASYAGGIPGLRATSPTVTGR
                     SLRSPSAAVAAYRSYVARLEQRVLDAIEARIPRSTVGSSFRIAYGGFALRLPADEVGE
                     LLAIPDVAAVQRDQLEQPLAVEEPYQSIGAQAVWNQLDGSVLTGQGVVVANLDTGIWP
                     ENPMFEDHGLPAPPGGPYACQFGLSGDSNDPAFACNHKVIGAKAFVDTYTTFIVAETG
                     EYCDTVGPDPNVVCSARDADGHGTHTLSTAAGDFVATAQMWGANWGPTSGMAPG"
BASE COUNT          378 a          893 c          882 g          340 t
ORIGIN      
        1 gcaaccggcc gggcccgcac gccggagccg atccgcaggg cccgtcacct ggcccgcctg
       61 gcccgggcgg gcgcggaggg ccgcctgcgg cgggactagc cgaccatccg tcggtcggcg
      121 tcgcgcgcgt gcggccaccg acgccacgcg tcgggcagca ccggcacgac gccgccacgg
      181 atcgccgccg cgagcgcacc cagggctcgc tcgcgccggg ggtcccacgc caggtcgtac
      241 gccgcgcgga tccgaggggg gagcagcccg gcggtcacga accgcatgag accggcaagc
      301 ggacggagcg cggtgggggt cctcggatcg aggacacctc gggagagcag ccgtgcttcg
      361 tcgctgacgt gcagcgtggc caccatccgg tccacgtatc gccggaagtc tccgagcgtg
      421 accggaaggc ggtcccgcgg aatgccgaag gccacggcct ggcgcttcat ctcctcgtgg
      481 tagcggcccc gcgcagcgcc gccgatcgac ccgacgaagc gctcgtacgt gacgatcgca
      541 ccgtccacga gtgtggcgtg gacccacagg aggagctcgg ggtccagcgc ccggtacgca
      601 cgtgggccgc ggctgccggt cacgagacgg tgggtctcgg tgacccgcga ggccgccgct
      661 cgacgctggt cctcgtcgcc gaatgccacg gtcagcgtca ggtcgagcgt gttcgccaga
      721 cggccgaagg cgtcccgccg gaagtcgctg tggtcggcca ctcccgcggc caccatcgga
      781 tgcgccagct ggagcagcag cgcccgcccg cccccgagca ggagggcggt ctcggcgttc
      841 actcgccccg ccaccgaccc gggtgggaac aacccgggat cggcgaggct tcctcgcccg
      901 gtccccggca gccatctccg aacgagatcg aacgtcacca tgcgtccgta gtgtcgcaac
      961 cgatgaccgc gtcagccggt cctcagcagg tgagatgcaa cgcggcggcg gtccgcgccg
     1021 gatcgagctc gccgaaccgg atcacggcgc ggtcggtggg gaggcactcg acgtcgacgc
     1081 cacgctcgcg gagacgctcc agggtggccg ggtcgggacg catctgcgac gacgccccca
     1141 tgcccaccac gaggcgttcc ggaagctcct cgaggacgcc ctcgaggtcc tgaaacacca
     1201 gcacgtggcc gtcccgccgt ctccagttcc gaacgatgcg tccgggcagc acgatgacgt
     1261 ctcgcgtctc cggccgcccg tcgaccacga gatgaccgaa cgaatagtcc gtgatcgtgg
     1321 ccatggagcc aggatgcctc ggcggccgca aagcgtcacc ccgtcgcctt ctcgtggtgc
     1381 gcctcagtct cgggccaatc ggcccgggac catcttgatg gcgccgtgac cacgctgcgc
     1441 gtttgcgcac tcaccggtcg aagggcaaga tttcgccacc cgtcaacggc aaggaggacg
     1501 tgatgaggcg tcggatcgtt cggcttgcgg ccgtgctggc cgtgatcatg tcggtcgtcg
     1561 gctcggtcgg cgcttccgcc tcgccgaaga ggggcctgcg gctcgcgccc gagagcgtgt
     1621 cgctcgcgag cctaatcgag gcgccgagat ccttgaccgg gaagctggcc gagaccgacc
     1681 cgagcctcct ggggaagacc agctccgagt tggtgaacgt gatggtgaag ctcgactacg
     1741 acccgatcgc ctcctacgcc ggcggcatcc ccggtctccg ggcgacgagc ccgaccgtga
     1801 ccggtcgatc gctccgcagc ccatcggcgg cggtcgccgc gtaccggtcc tacgtggcga
     1861 ggctcgagca gcgagtcctc gacgccatcg aggccaggat ccctcggtcg acggtcgggt
     1921 cctcgttccg gatcgcgtac ggaggcttcg ccctccggct cccggctgat gaggtggggg
     1981 agctgctcgc gatcccggac gtggcggccg tgcagcggga ccaactcgag cagccgctcg
     2041 ccgttgagga gccgtatcag tcgatcgggg cgcaggccgt gtggaaccag ctcgacggat
     2101 ccgtgctcac gggccaggga gtcgtggtcg cgaacctcga cacgggcatc tggcccgaga
     2161 acccgatgtt cgaggaccac ggccttccgg ctcctccggg cggaccgtat gcctgccagt
     2221 tcggcctgtc gggtgattcg aacgaccccg cgttcgcgtg caaccacaag gtgatcggag
     2281 ccaaggcctt cgtcgacacg tacacgacgt tcatcgtcgc cgagacaggc gagtactgcg
     2341 acacggtcgg cccagacccg aacgtggtct gctcggctcg tgacgccgac ggccacggca
     2401 cgcacaccct ctcgaccgcc gccggggact tcgtggcgac ggcacagatg tggggcgcga
     2461 actgggggcc gaccagcggc atggcgccgg gag
//
LOCUS       DASPWO010000567         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_420910, whole genome shotgun sequence.
ACCESSION   DASPWO010000567 DASPWO010000000
VERSION     DASPWO010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420910"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2096)
                     /locus_tag="VEO00_12980"
     CDS             complement(<1..2096)
                     /locus_tag="VEO00_12980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYU58943.1"
                     /translation="MASTSTVARSSTESSDRSLSVGAHVLILVGALAITLVGVGAFQT
                     SNAFRGARQDAGLDTRFQARLAADALAASMADAQAFAAGTAANPAIVQVFETIDGRYE
                     LCSLAGSGTGLFDRGLIHFLDRTGFLFCSSLRQRIHERDLHYDGFGWFRRSLDLQDGL
                     PLRTDPFIDPSISELTVAFAAPVRNAGGEAIGVVVYSLPVSGLAPQMESTYGGREHAE
                     FDVTRTGTGQLLSSSEGDAARAPSGPSRLASPPSEAIPGLDGTRRIYRAEPVGDSGFT
                     VTAGNSEATILADARRELWAHASLTLLALVVTLAFGFLVNRRIGRPIRTLTVAVEQAG
                     RAVDPEPVAVDGPREIRRLVEGFNGMLAARAAVEEQLTERALFDPLTGLPNRVVALDR
                     LGHALEGTRRGPGLVAVLSIDLDRFGIVNDSFGRDVGDRMLISVAGRLRSVLRPQDTL
                     ARFYGDEFIAVCEDIVSREDAVAVAEGIAASLRRPIREGTAEATLSASIGIAFGRHDQ
                     DTAETLLRDADAAMSVAKGRGKGRHVFFEPTMRERVRNRLDLENDLRRAIERDELHLS
                     YQPIVDVRSGRIVGAEALVRWHHPTKGVLLPGVFINLAEETGLILPIGRRVLDQACAQ
                     AAEWSLQGHPIGVSVNLSPRQIAEPGLVEDVRSSLRAADLEPSRLRVEVTESTLMRQD
                     AAREVLERMKELGVGI"
     gene            complement(2220..>2644)
                     /locus_tag="VEO00_12985"
     CDS             complement(2220..>2644)
                     /locus_tag="VEO00_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58944.1"
                     /translation="NRPQSSADIGISLSMDGGLTWSDAHVLSVTNTGAPAPNDQFFPW
                     IDSDESGNFVAMWYDRRLDPGNKLIDEWQAVSTDDGRTWTTRKISTESWDSSRYGFIG
                     DYIGLAASDAAIYPVWTDGRSAFFPGESDIWSNVEIRG"
BASE COUNT          404 a          935 c          914 g          391 t
ORIGIN      
        1 ctgatcccga cccccagctc cttcatccgc tccagcacct cccgggcggc gtcctgccgc
       61 atcagggtgc tctccgtgac ttcgacccgg agtcgggagg gctccaggtc ggcggcgcga
      121 aggctcgatc ggacgtcctc caccagtccg ggctcggcga tctgcctcgg cgaaaggttc
      181 accgagacgc cgatcggatg tccctggagg ctccattcgg ccgcctgcgc gcaggcctgg
      241 tcgaggaccc gccgcccgat cgggaggatc agcccggtct cctccgccag gttgatgaag
      301 accccgggga gcaggacccc cttcgtcgga tggtgccagc gaaccagggc ttcggccccg
      361 acgatgcggc ccgatcgaac gtccacgatc ggctggtagg acaggtgcaa ctcgtcgcgt
      421 tcgatcgccc gcctcaggtc gttctccaga tcgagccggt tccgaacacg ctcccgcatc
      481 gtcggttcga agaacacgtg ccgccccttg ccccggccct tcgcgacgga catcgcggcg
      541 tcggcgtcgc ggagcagcgt ctcggctgtg tcctggtcgt ggcggccaaa cgcgatcccg
      601 atcgaggccg acagggtggc ctcggcggtt ccctcccgga tcgggcgccg cagcgacgcg
      661 gcgatccctt cggccacggc gacggcatcc tcgcgggaca cgatgtcctc gcacacggcg
      721 atgaactcgt cgccgtagaa gcgggcgagc gtgtcctggg gccgaagcac ggagcgcagg
      781 cggcccgcca cggagatcag catgcggtcc ccgacgtcgc gcccgaagct gtcgttgacg
      841 atgccgaacc ggtccaggtc gatcgacagg acggcgacga ggccaggccc ccgtcgggtg
      901 ccttccaggg catggcccag ccggtccagc gccaccaccc ggttcgggag gccggtcagc
      961 ggatcgaaca gggctcgctc ggtgagctgc tcctccacgg cggcccgggc cgccagcatc
     1021 ccgttgaacc cctcgaccag ccgccggatc tcccgcggac cgtcgaccgc gacgggttcg
     1081 gggtccaccg cgcgccccgc ctgttccacc gcgacggtca aggttcggat ggggcgaccg
     1141 atccgccggt tcaccaggaa gccgaaggcc aaggtcacca ccagcgccag gagcgtcagg
     1201 ctggcgtggg cccacagttc tctccgggca tccgccagga tcgtcgcctc cgagttgccc
     1261 gccgtcacgg tgaagccgga atcgccgacg ggctcggccc ggtagatgcg ccgggtgccg
     1321 tcgaggccgg ggatcgcctc ggatggcggg ctggccaacc gggacggtcc ggacggggca
     1381 cgggccgcgt ccccttcgga cgatgacaag agctggccgg tccccgtgcg cgtcacgtcg
     1441 aattcggcgt gttcgcgccc cccgtacgtg gactccatct gcggggcgag gccgctcacg
     1501 ggcaacgagt agaccaccac accgatcgcc tctcctcccg cgttcctgac cggggccgcg
     1561 aaggcgaccg tcagctcgct gatggaaggg tcgatgaagg gatccgtccg gagcggaagg
     1621 ccgtcctgaa gatcgagcga ccgccggaac cagccgaaac cgtcgtagtg gaggtcgcgc
     1681 tcgtggatcc gctggcgcag ggaggagcag aacaggaacc cggttcggtc caggaagtgg
     1741 atgagcccac gatcgaagag acccgtgccg gaaccggcca gggagcacag ctcgtagcgt
     1801 ccatcgatcg tctcgaagac ctgcacgatg gcgggattgg cggccgtgcc ggcggcgaat
     1861 gcctgggcgt ccgccatcga cgcggccagg gcgtcggccg ccagccgggc ctggaaccgg
     1921 gtgtccagac ccgcgtcctg gcgagcgccc cggaaggcgt tcgaggtctg gaacgctccc
     1981 accccgacga gcgtgatggc gagcgcgccg accaggatga gcacgtgcgc gccgacggac
     2041 agggaccgat cgctcgattc cgtgctcgat cgagcgacgg tggacgtgct ggccatgttc
     2101 tcctcagtac gcatccggtc tgggcggtcc cgaggcgcgg gctcccgtct ggttatcggg
     2161 cgtcgacaac cgaccctgta gggctgccct ccacgagaac cggccgagcc ggggggagct
     2221 cagccgcgga tctcgacgtt cgaccagatg tcgctctcgc cggggaagaa ggcgcttcgc
     2281 ccgtcggtcc acaccgggta gatcgccgcg tcgctcgcgg cgagcccgat gtagtccccg
     2341 atgaatccgt accggctcga gtcccagctc tcggtgctga tcttcctcgt cgtccacgtc
     2401 cggccgtcgt cggtggatac ggcctgccac tcgtcgatca gcttgttccc gggatcgagg
     2461 cgccggtcgt accacatcgc cacgaagttc cccgactcgt ccgagtcgat ccacgggaag
     2521 aactgatcgt tgggggcggg cgcgccggtg ttcgtcacac tcaggacgtg ggcgtcgctc
     2581 cacgtcaggc cgccgtccat ggacaacgag atgccgatgt ccgcactcga ctggggccgg
     2641 ttga
//
LOCUS       DASPWO010000568         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_420932, whole genome shotgun sequence.
ACCESSION   DASPWO010000568 DASPWO010000000
VERSION     DASPWO010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420932"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VEO00_12990"
     CDS             <1..346
                     /locus_tag="VEO00_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HYU58945.1"
                     /translation="YLVIGAMVAATLKSVVPAELMQALADNPVLSVLALAVLAVVLSI
                     CSEADAFVAASLTQFSLSARLTFMVVGPMVDLKLIALQAGTFGRRFALRFAPLTLAVA
                     VATSALVGWWLL"
     gene            334..1038
                     /locus_tag="VEO00_12995"
     CDS             334..1038
                     /locus_tag="VEO00_12995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HYU58946.1"
                     /translation="MAAVTAAGGTISLLAGTVVLRLTLTGAYRRYVRVGMWPWLLAAG
                     LAIVVLGAATLVTAMRHGPAEDAHGHGRRGEQRVGWLLLAPIAALLLVAPPPLGAYGV
                     GRGAAVDVRAGAEGFGFGRLDPGDEPVPMTLLEFGQRSVDRDGVSFNGVAVRLTGFVA
                     GAEHHGFLLARYEIACCAADAVPVVIRVVGVPGPAPPNDRWVTVTGLFRPGGGTTPWL
                     AATAVAEVPAPRDPYE"
     gene            complement(1029..>2618)
                     /locus_tag="VEO00_13000"
     CDS             complement(1029..>2618)
                     /locus_tag="VEO00_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HYU58947.1"
                     /translation="VIERFLRPAEADAAFAGLRERWSETLGALQVVTPNEHANRMVNV
                     WNAYQCMVTFNLSRSASFFESGVGRGMGFRDSNQDLLGFVHMAPARARERLLDLAATQ
                     LPSGGAYHQYQPLTKRGSDAIGSGFNDDPLWLILAVAAYLKETGDGTILAEPVPYDNA
                     LGSEAPLDEHLQRAVGYTLDRLGPHGLPLIGHADWNDCLNLNAFSEAPGESFQTAHNR
                     EGGVAESVFIAALFTLAAGELAAIGERFGRPRDAAGYRDAARQMDASVRAHGWDGAWF
                     RRAYDHFGNAIGSAENEEGQIYVEPQGLCAMAGIGVDDRTAERALASVAERLATPHGI
                     VLLQPAYTRYRLELGEITSYPPGYKENAGVFCHTNPWIVIAEAILGNGDRALDYYLRI
                     NPSAREAISDVHRCEPYVYAQMIAGRDAPDPGEAKNSWLTGSAAWNLVAISQWILGIR
                     PEHDGLRVDPCLPADWDGFEATRRFRGATYRIRVRAQAGARGRVTRLVVDGRPLEGTV
                     VPPAAAGATVEVEATIGPHYS"
BASE COUNT          307 a          961 c          957 g          393 t
ORIGIN      
        1 gtacctcgtc atcggcgcga tggtggcagc caccctgaag tccgtggttc cggccgaatt
       61 gatgcaggcg ctggccgaca accccgtgct gtcggtgctg gcgctggccg tcctcgcggt
      121 ggtcctgtcg atctgctccg aggccgacgc gttcgtcgcc gccagcctga cccagttctc
      181 gctctccgcg cgcctgacat tcatggtcgt cggcccgatg gtcgacctga agctcatcgc
      241 ccttcaggcc gggacgttcg gacggcgctt cgcgctgcgc ttcgcgcccc tgacgctggc
      301 cgtggcggtg gcgacgagcg ccctggtcgg gtggtggctg ctgtgaccgc cgccggcggg
      361 acgatcagct tgctggccgg cacggtggtg ctgcggctca cgctgaccgg cgcctaccgg
      421 cgctacgtcc gcgtcgggat gtggccgtgg ctgctcgccg ccggcctggc gatcgtcgtt
      481 ctcggggcgg cgaccctcgt gaccgcgatg cgccacggcc cggccgagga cgcccacggc
      541 cacgggcggc gcggggagca gcgggtgggg tggctgctcc tggcgccgat cgccgcgctg
      601 ctcctcgtcg cgccgccccc gctcggcgcc tacggggtcg gccgcggggc cgcggtggac
      661 gtccgggccg gggccgaggg gttcgggttc ggtcggctcg atcctggcga cgagcccgtc
      721 ccgatgaccc tgctcgagtt cgggcagcgg tccgtcgacc gcgacggcgt cagcttcaac
      781 ggcgtcgccg tgcggctgac cggcttcgtc gcaggcgccg agcaccacgg cttcctcctg
      841 gcccgctacg agatcgcctg ctgcgcggcc gacgccgtcc cggtggtgat ccgggtggtc
      901 ggcgtcccgg ggccggcgcc gccgaacgat cggtgggtca cggtgaccgg gctgttccgc
      961 ccggggggcg gcacgacgcc gtggctcgcc gccacggcgg tcgccgaggt cccggcgccc
     1021 cgggacccct acgagtagtg gggtccgatc gtcgcctcca cctccacggt cgctccggcg
     1081 gccgccggcg ggacgacggt gccctccagc ggccggccgt cgaccacgag tcgcgtcacg
     1141 cggccccgag cgccggcctg cgcgcggacc cggatccggt aggtcgcgcc ccggaaccga
     1201 cgggttgcct cgaacccgtc ccagtccgcc ggcaggcacg ggtcgacgcg caggccgtcg
     1261 tgctccgggc ggatcccgag gatccactgc gagatcgcga ccaggttcca ggcggccgag
     1321 cccgtcagcc aggagttctt cgcctcccca gggtccggcg cgtcgcgccc ggcgatcatc
     1381 tgcgcgtaga cgtacggctc gcagcgatgc acgtcggaga tcgcctcgcg ggccgagggg
     1441 ttgatccgca ggtagtagtc gagcgcgcgg tcgccgtttc cgaggatcgc ctccgcgatc
     1501 acgatccacg ggttcgtgtg gcagaacaca ccggcgttct ccttgtagcc cggcgggtac
     1561 gacgtgatct ccccgagctc caggcggtag cgcgtgtagg ccggctgcag gaggacgatg
     1621 ccgtgcggtg tggccaggcg ctcggcgacc gacgccagcg cccgctcggc cgtccggtcg
     1681 tcgaccccga tcccggccat ggcgcacagc ccctgcggct cgacgtagat ctgcccctcc
     1741 tcgttctccg cggacccgat cgcgttcccg aaatggtcgt aggcgcggcg gaaccaggcc
     1801 ccgtcccacc cgtgcgcgcg cacggacgcg tccatctgcc gggccgcgtc gcggtagccg
     1861 gctgcgtccc tgggtcgccc gaaccgctcg ccgatcgccg cgagctcgcc ggccgccagc
     1921 gtgaacaggg ccgcgatgaa gacggactcg gccacgccgc cctcccggtt gtgggccgtc
     1981 tggaacgact cgccgggagc ctccgagaac gcgttcaggt tcaggcaatc gttccagtcc
     2041 gcgtggccga tcagcggcag tccgtgcggg ccgaggcggt cgagcgtgta tcccaccgcg
     2101 cgctgcaggt gctcgtccag gggcgcctcc gacccgagcg cgttgtcgta tggaaccggc
     2161 tcggcgagga tggtcccgtc gccggtctcc ttcaggtagg ccgccacggc gaggatcagc
     2221 cacagcgggt cgtcgttgaa cccggagccg atggcgtcgc tgccgcgctt cgtcagcggc
     2281 tggtactggt ggtaggcgcc ccccgacggg agctgcgtgg ccgccaggtc gagcaagcgc
     2341 tcgcgcgcgc gggccggcgc catgtgcacg aaccccagga ggtcctggtt cgagtcccgg
     2401 aagcccatcc cgcgcccgac ccccgattcg aagaacgacg cggaacgaga gaggttgaag
     2461 gtgaccatgc actgataggc gttccagacg ttgaccatcc ggttggcgtg ctcgttcggg
     2521 gtcacgacct gcagggcgcc gagcgtctcc gaccagcgct cgcgcagccc ggcgaacgcc
     2581 gcgtctgcct ccgcggggcg gaggaatcgc tcgatcac
//
LOCUS       DASPWO010000569         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1010653, whole genome shotgun sequence.
ACCESSION   DASPWO010000569 DASPWO010000000
VERSION     DASPWO010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010653"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VEO00_13005"
     CDS             complement(<1..204)
                     /locus_tag="VEO00_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58948.1"
                     /translation="MAEEGPDRSATGRRLLLFYPLLFAAVAAVVAVSISAGSDEGTQP
                     EIAGGYDLAEQDPCLGSEFDVKQS"
     gene            269..2068
                     /locus_tag="VEO00_13010"
     CDS             269..2068
                     /locus_tag="VEO00_13010"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYU58949.1"
                     /translation="MPPASPPPEALAPTEVIAYVLADLAIILLAARIVGGIFVRIRQP
                     RVVGEIIAGILIGPTVIGGQLASGAITATDKPAVDGTGLVNDLFPLQAFSFLSLIGTL
                     TLVFFMFLVGLEVQQRFLKGRERQILIVALAVIAAPVALGFLIGSILDGADWKVAVTP
                     DGKSVSASTHALFLGAALAVTAFPVMARILQEKRMLSTPMGAIGVGAAAVVTPLMFIV
                     VAAALASAKGEGVPNTVGVKILLAVGLTALLFFVVRPLLRLILERRFDPAKPLDDELF
                     ALLLFGALATGLAADRIGINALNGGFLFGACVPQFEGLGRAVIDRLQQFVVVFMIPIF
                     LAVSGLQTDLRVLEVDLIGGILLFLVAMIVAKLAVGALAGRAVGLNWREANTIGTLMN
                     CRGLMILVVAVIGKQAGVITDPMQVTFVIGAIVTTMMTGPLVDALVPPVDVEQERDKS
                     VSGSLAGIPAMTGGPRVLIAPGQVDAAPAALEMAREYLSREGPAPQFLVADLPGLAAD
                     GDYVGAGPEEEETAVRRTLAWIRPLAEGLRAAGADAETASFQSPDPAADLAKLAAEWA
                     ATDAIVGPAEAAAQLEAGGVKVHRAGAATPALA"
     gene            complement(2065..>2256)
                     /locus_tag="VEO00_13015"
     CDS             complement(2065..>2256)
                     /locus_tag="VEO00_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HYU58950.1"
                     /translation="GIAYGLDRFVQRLRGADSIREVIAFPKTASGADPLTGAPARVDE
                     AQLRELGLALRPAQRRAPS"
BASE COUNT          311 a          793 c          788 g          364 t
ORIGIN      
        1 cgactgcttg acgtcgaact cgcttcccag gcacgggtcc tgctcggcca ggtcgtagcc
       61 gcccgcgatc tccggctggg tgccctcgtc ggagcccgcc gagatgctga ccgctaccac
      121 ggccgcgacg gcggcgaaga gcagcgggta gaagaggagg aggcgccgtc cggtggcgct
      181 ccggtccgga ccctcctcag ccatcgcgcc gcagcgtatc ggggaaaaag cgttgcgacc
      241 gcggctcggt cgcgaatagg atcgcgccgt gccgccggcc tcaccgccgc ccgaggcgct
      301 cgcgccgacc gaggtgatcg cctacgtgct cgcggacctg gcgatcatcc tgcttgccgc
      361 tcggatcgtc ggcgggatct tcgtccgcat ccgtcagccg cgggtcgtcg gcgagatcat
      421 cgcggggatc ctgatcgggc ccacggtgat cggcgggcag ctcgccagcg gcgccataac
      481 ggcaaccgac aagccggcgg tggacggcac cggactggtc aacgacctgt ttcccctcca
      541 ggccttctcg tttctgagcc tgatcggcac gctcacgctg gtcttcttca tgttcctggt
      601 cggactcgag gtacagcagc gcttcctcaa ggggcgcgag aggcagatcc tgatcgtggc
      661 gctcgccgtg atcgccgcgc cggttgcgct tggcttcctg atcggctcga tcctcgatgg
      721 cgccgattgg aaggtggcgg tgacccccga cggcaagtcc gtctcggcct cgacacacgc
      781 gctttttcta ggcgccgcgc tcgccgtcac cgcgtttccg gtgatggccc gaatacttca
      841 ggaaaagcgg atgctctcga cgccgatggg cgcgatcggg gtcggcgcgg ccgccgtggt
      901 aacgccactg atgttcatcg tcgtcgccgc agcgctcgcc tccgcgaagg gagagggggt
      961 ccccaacacg gtcggggtga agatcctcct ggcggtcggg ctcaccgccc tcctcttctt
     1021 cgtcgtacgc ccactcctgc ggctgatact ggagcgcaga ttcgaccccg ccaagcccct
     1081 cgacgacgag ttgttcgccc tgcttctgtt cggcgcactg gccaccggcc tcgccgccga
     1141 cagaatcggc atcaacgcgc tcaacggcgg cttcctcttc ggcgcgtgcg tgccccagtt
     1201 cgaggggctc ggccgggccg tgatcgatcg tctgcagcag ttcgtcgtcg tgttcatgat
     1261 cccgatcttc ctcgccgtgt cggggctcca gacggacctc cgcgtgctgg aggtcgattt
     1321 gatcggcggc atcctgctct tcctggtcgc gatgatcgtc gccaagctcg cggtgggagc
     1381 cctcgccggg cgggccgtgg gactgaactg gcgcgaggcg aacacgatcg gcacgctcat
     1441 gaattgccgc ggcctgatga tcctggtcgt cgccgtgatc ggaaagcagg ccggggtgat
     1501 caccgacccg atgcaggtca ccttcgtcat cggggcgatc gtcaccacga tgatgactgg
     1561 gccgctcgtg gacgccttgg tgcccccggt cgacgtcgag caggagcgcg acaagtcggt
     1621 gagcggatcg ctcgccggga tcccggcgat gaccggaggg ccgcgcgtgc tgattgcccc
     1681 tgggcaggta gacgcggcac cggccgccct cgagatggcc agggagtacc tgagccggga
     1741 gggtccggcc ccgcagtttc tggtggcgga ccttcccggg ctcgccgccg acggcgacta
     1801 cgtcggggcc gggcccgaag aggaggagac cgcagtgagg cgcaccctgg cctggatccg
     1861 tcccctggcc gagggcctca gggcagccgg ggcagacgcc gagacggcct ccttccagag
     1921 cccggatccc gccgcggacc tggccaagct cgctgccgag tgggcggcga ccgacgccat
     1981 cgtcggaccc gctgaggcgg ctgcgcaact cgaagccggc ggcgtcaagg tgcaccgggc
     2041 cggcgccgcg acgcccgccc tggcctagct cggggctctg cgctgcgccg ggcgcaaggc
     2101 gagccccagc tcgcgaagct gcgcctcgtc gacccgcgcg ggggcgccgg tcagggggtc
     2161 cgcacccgag gcggtcttcg ggaacgcgat cacctcacgg atcgagtcgg ccccgcgcag
     2221 ccgctggacg aagcggtcca gcccgtaggc gatccc
//
LOCUS       DASPWO010000570         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_503309, whole genome shotgun sequence.
ACCESSION   DASPWO010000570 DASPWO010000000
VERSION     DASPWO010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503309"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..473
                     /locus_tag="VEO00_13020"
     CDS             63..473
                     /locus_tag="VEO00_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYU58951.1"
                     /translation="MTGGRTSGRAELALADTSVFIALEQERPLSGSPPEAVAVSVITV
                     GELRLGVLAASDGPTRARRLETLSRAEGLEPLPIDTAVAHAWAALRLALRDAGKRMPL
                     NDSWIAATAIANRIPVVSQDDDYDEVPGLQVIRV"
     gene            complement(836..>2205)
                     /locus_tag="VEO00_13025"
     CDS             complement(836..>2205)
                     /locus_tag="VEO00_13025"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HYU58952.1"
                     /translation="PLQEDIPSLVVPAMEAIGATSVLDPTPGPDLPALARLLVLGAGV
                     HHTKTFRDGDVLYFRNYASAGALYPIEVYVGCADLPGLPAGVYHFDPQGPSLTRLREG
                     DHRGHLVRATAGEPAVARAPVVLALTRIPWRTAWKYTERGYRHLFWDAGMILANVLAL
                     AASVRLPVRVVLGFADAEVSVLLGLEERREFPLCLVAIGTADSEVPPAAKPPEEVSFR
                     VLPLSQTEHVHQGILQANDAGRLDAPKEARTWRRSRGGDEEVERTPPLSSDTARDSLE
                     DVLRRRGSARMFAPGAIPATVLRTILERATVGIPTDYEPAGARLVEPYLIANAVEGLD
                     PGAYLFRDGELRLLDRGNFRREAEFLCLEQELGATAAATHFLMADLPRILDALGGRGY
                     RTAQLEAGIVAGRLYLGAYAHRFGATGLTFYDDAVAEFFSPDTAGKSCVLVTAIGESP
                     RLRRA"
BASE COUNT          359 a          733 c          792 g          321 t
ORIGIN      
        1 gcggcgttgg ctcgggagtt ggcagacctg cttcctgaga ccaccgacga cgttccgatc
       61 ccgtgaccgg cgggcgaacc agtggacgcg ccgagctggc cctcgctgac acgtcggtct
      121 tcatcgccct cgaacaggaa aggcccctgt ccggctcacc gcccgaagcc gtcgcggtgt
      181 cggtgatcac ggtgggggag ctccgactcg gcgtcctggc cgcgagcgat ggaccgaccc
      241 gagcgcgccg actggagact ctctcccggg cagagggcct cgagccgctc cccatagata
      301 cggctgtcgc tcatgcctgg gcggctttgc gcctggcact gcgagacgcc ggcaagcgga
      361 tgccgctcaa cgattcgtgg atcgcggcga cggcgatcgc aaaccgtatc ccggtcgtct
      421 ctcaagacga cgactacgac gaggtcccgg gactgcaagt catccgggtc tgaccaccgc
      481 ttctactcca agggatactc ccacgaaatg gagtcgacga gtacctgggc cgactgtgtc
      541 tctgacggcc gccaatgcga agccccccat ccgccgaacg cgacaaccta gaggttctgc
      601 ccctatgtcg gaggtccctc ggctcttcgc cccaggcgat cctgtagcag caggcacggg
      661 cctcggtgag ctcgactccg gcataatcag aatcccccgg tcaggggttc atgtaccaag
      721 atcctcaggt acaccctcga atgggtctag ggaaagggaa cggcgccgcc tcggttaggg
      781 gcgccgggcc ggaccggggc ccgcctcaga gggcctttcc tgattcgccg ccggatcagg
      841 cgcgtcggag gcgaggactc tcgccgatcg ccgtgacaag cacgcagctc ttgcccgcgg
      901 tgtcgggcga gaagaactcg gcaactgcgt cgtcgtagaa cgtgaggccg gtggcaccga
      961 aacggtgcgc gtacgcgccc aggtataggc gcccggcgac gatgcctgcc tccagctgcg
     1021 cggtgcggta gccgcggcct ccgagcgcgt cgagaatcct cgggaggtcc gccatcagga
     1081 agtgggtcgc tgccgccgtc gcgccgagct cctgctctag gcagaggaac tcggcctccc
     1141 ggcggaagtt gccccggtcc aggagccgga gttccccgtc gcggaagagg taggcgcccg
     1201 gatcgaggcc ctcgactgcg ttcgcgatga ggtacggctc cacaagacga gcgccggcag
     1261 gctcgtagtc ggtcgggata ccaaccgtgg cccgttcaag gatcgttcgg agaaccgtgg
     1321 cgggaatcgc gccgggcgcg aacatccggg cagagccgcg ccgacggagg acgtcctcga
     1381 gggagtcgcg cgccgtatcg gatgagagcg gtggagtccg ctcgacctcc tcatcgccgc
     1441 ctctgctgcg ccgccacgtc cgagcctcct tcggagcgtc caagcggccg gcgtcgttcg
     1501 cctggaggat gccctggtgg acgtgctcgg tctgtgacag cggcaggacc ctgaacgaga
     1561 cctcctcggg cggcttcgcg gcgggtggga cctctgagtc cgcggttccg atcgcgacca
     1621 ggcagagcgg gaactcgcgc cgctcctcca gcccgagcag cacggagacc tcggcgtcgg
     1681 caaaccccaa gaccacgcgg accgggaggc ggaccgaggc cgccagggcc aggacgttgg
     1741 ccaggatcat gccggcgtcc caaaacaggt ggcggtagcc gcgctcggtg tacttccacg
     1801 cggtccgcca ggggatgcgg gtgagggcga gcaccaccgg ggcccgggcc acggcaggct
     1861 ctcccgcagt cgctcggaca aggtgacctc ggtggtcgcc ttcccggagg cgggtgaggc
     1921 tcggaccttg cgggtcgaag tggtacacgc cggcgggaag gccggggagg tcggcgcagc
     1981 ccacgtagac ctcgatcggg tacagggccc cggcggaggc gtagttgcgg aagtacagga
     2041 catcgccgtc gcggaacgtc ttcgtgtggt ggacgccggc gccgagcacg aggagtcggg
     2101 ccagggccgg gaggtccggg cctggcgtgg ggtcgaggac ggacgtggca ccgatcgcct
     2161 ccatggccgg aacgacgagg gagggtatgt cctcctgcag cggga
//
LOCUS       DASPWO010000571         5217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1264406, whole genome shotgun sequence.
ACCESSION   DASPWO010000571 DASPWO010000000
VERSION     DASPWO010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264406"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1624)
                     /locus_tag="VEO00_13030"
     CDS             complement(<1..1624)
                     /locus_tag="VEO00_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58953.1"
                     /translation="MSAVETLVGHDWRLVAPWWHWPRRDDPGAGGSRGRFTRPVLQKY
                     DTPDLVNTFLRDPQLRLQLLDAADRVAVVTRTRSRWSRPTSTTTELRKLYLPTHHRNY
                     LVVCSLHCLGAGFPVVGERAEVCEAGFVVRRRTYDLDDGDRAAAVDLLRRSTVSRARM
                     LDADRQLDSAKRVGVPSVRLAKLDARRSALGRVADDAAGRVRTWARDAGVKRALKGWI
                     PSGLHPDGSVGPMPACPPATGGPSPLAGVGRWRDVGETPTELQEATFPLYPLVPDPTQ
                     PDHDGAGAAAWFGVVPTGSADLTFTGRPRFDDTHVYEIRCFVRRHRAVCPRTGPQCTC
                     PIVWSEPTEPYQLASHFDLQGTANRPVTVQLPDIKALQADANRLGPGRTGGVRMKAPA
                     NSNLMFDVDTTAGKGSNGGTGGPEICTFAIPLITIVATFVFKLFMPIVIFLFQLWFLL
                     SLRFCIPPEISIDAGFAAALDALPPDLQIDASAAASFDATWGSKLDAAVNAAAGGMKA
                     GGQTAAAALRSSLGPAERLRAQRGIIQAAAASG"
     gene            complement(1621..2283)
                     /locus_tag="VEO00_13035"
     CDS             complement(1621..2283)
                     /locus_tag="VEO00_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HYU58954.1"
                     /translation="MTGAREAGDAGRFFGVYPAIVTDIVDPDRLGRVEVRFPWLGSAG
                     DQVRAWATLLTPYADDDQGFVAFPAVDTQVVVAFEAGDVRRPYIVGSCWNGKEMVPVT
                     PTKPNDKRVIKSRSETLLEFDDGPSGPKLTIKTKQGHLLVLDEGTQKIELTHMNGPTV
                     TLNAAGQVEIKANATVEITASALNVHAATAVFDGIISCTTLNASNGVVSPSYTPGAGN
                     VW"
     gene            complement(2280..3368)
                     /locus_tag="VEO00_13040"
     CDS             complement(2280..3368)
                     /locus_tag="VEO00_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HYU58955.1"
                     /translation="MADRPRYASTSPVVKVDGNRVADLARDLLRLDVQEDTCGLRTLT
                     LHLVASAARSTPTTDVAEYLDGTVLDFGKKVEVSMGPPGNEKVVFTGKVSALEVSFEE
                     GDVPHVTALAEDELMLLRMTQRSSTYRNVTDGDVAQSIAGKHGLRPEIAANGPTYDVV
                     QQLNQSDLAFLRERASRVSAELWAADGTLHFATRDQRQGTTLTLTQGSNLLAASVRAD
                     LAHQCTGINVSGYDAKSRDRVDVEGASSVVSAEANGGRTGPDVLQKAFGAMAGQETRL
                     VPFVDADARAFAEAEMLRRARGFVTIEGTTDGSPEMVVGSRLKLGRVGKPFEGNGYYV
                     TRVHHTYDLTRGHRTHFLAERPTIGAGT"
     gene            complement(3361..3549)
                     /locus_tag="VEO00_13045"
     CDS             complement(3361..3549)
                     /locus_tag="VEO00_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58956.1"
                     /translation="MSVRVGELTSEVQVTGRGDGSPEGRGPDGKLQPSWNDRERHLAL
                     ERAMRRDECRTEARRFDG"
     gene            complement(3546..4847)
                     /locus_tag="VEO00_13050"
     CDS             complement(3546..4847)
                     /locus_tag="VEO00_13050"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58957.1"
                     /translation="MEFKKASLQEVDSKPSPTPATVGEEIKVQINPASLRLQLANNVD
                     MGKAFARPNTQYQGSSSSTLSFDLIFDTADEGTTEVPVDVRTRTRQLERFMLPKKDAK
                     AVPPRVLFKYGTLQVVGVMSAMSQEFDLFTQNGVPLRAKCAVTIKEQKPEFDAGLQGP
                     GANTGAGATAPIPPQAGSAPRPPDRTGTALAGESASDFANRMGLDPKAWKGLDGIANP
                     LSLAAGALIDFSSSLSADVGLGVEVSATAGIDAGGSPGGGPGGGAAPAAPPGSAPPGA
                     VPPPGPPPVPTDGGALTAEGGLSVAAARATATRATAAADAARRAFPASPTPATPPAAP
                     AGVPAAATTGVPGVDGRTTSFGFGVPLRPRAGGRTAATVALVHARFPGRVAASVGDVP
                     VTDDPTVPSWQALPPAPAAGAAAARSRHGFASCGCGCGGVR"
     gene            complement(4847..>5217)
                     /locus_tag="VEO00_13055"
     CDS             complement(4847..>5217)
                     /locus_tag="VEO00_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HYU58958.1"
                     /translation="LDVEMDVSEIQEGGRNNAVIQRAGRAKFTRLTLKRGMLHPSDGT
                     VASDLWGWLQDVVDGVRPIRRYDGTVLVLGVGERPVATWSFARGLPAKIVGPQLNART
                     GEVAVEELQIAHEGLRMVIR"
BASE COUNT          664 a         1913 c         1913 g          727 t
ORIGIN      
        1 cgccgctcgc ggcggccgcc tggatgatcc cgcgctgcgc gcgcagccgc tcggccggcc
       61 ccagcgagga ccggagcgcg gccgccgccg tctgcccgcc cgccttcatc ccgcccgcgg
      121 cggcgttcac ggccgcgtcg agcttcgagc cccacgtcgc gtcgaacgag gccgcggccg
      181 aggcgtcgat ctggaggtcg ggcggcagcg cgtcgagcgc ggcggcgaag cccgcgtcga
      241 tgctgatctc cggcgggatg cagaagcgca gcgacagcag gaaccacagc tggaacagga
      301 agatcacgat cggcatgaac agcttgaaga cgaacgtcgc cacgatcgtg atgagcggga
      361 tggcgaacgt gcagatctcc gggccgccgg tgccgccgtt cgaccccttc ccggcggtcg
      421 tgtcgacgtc gaacatcagg ttcgagttcg ccggtgcctt catgcggacg ccgccggtcc
      481 gcccgggccc gagccggttc gcatccgcct gcagggcctt gatgtcgggg agctgaaccg
      541 tcacgggacg gttcgcggtt ccctggaggt cgaagtggga tgcgagctgg tagggctcgg
      601 tcggctcgct ccacacgatc gggcaggtgc actgggggcc ggtccgggga cagaccgccc
      661 ggtgccggcg cacgaagcac cggatctcgt acacgtgggt gtcgtcgaac cgcggcctcc
      721 cggtgaacgt gaggtcggcg gacccggtgg gaaccacgcc gaaccaggcc gcggcgccgg
      781 cgccgtcgtg gtccggctgc gtcgggtccg ggaccagcgg atacagcggg aacgtggcct
      841 cctgcagctc cgtcggggtc tccccgacgt cccgccaccg gcccaccccg gcgagcggcg
      901 acggcccgcc ggtggccggc ggacaggccg gcatcggccc tacgctcccg tccgggtgca
      961 ggccgcttgg gatccatccc ttgagggcac gcttgacccc ggcgtcccgc gcccaggtcc
     1021 ggacgcggcc ggccgcgtcg tcggcgaccc ggccgagcgc ggaccggcgg gcgtcgagct
     1081 tcgcgaggcg caccgaagga acgcccaccc tcttggccga gtcgagctgc cggtcggcgt
     1141 ccagcatccg ggccctcgac accgtggagc gccggagcag gtcgacggcg gccgcccggt
     1201 cgccgtcgtc gaggtcgtac gtccgccgcc gcaccacgaa gccggcctcg cacacctcgg
     1261 cgcgttcgcc cacgacgggg aaccccgccc ccaggcagtg cagggagcac accaccaggt
     1321 agttgcggtg gtgtgtcggc aggtagagct tgcggagctc ggtggtggta ctcgtgggcc
     1381 gcgaccaccg agaccgcgtc cgggtgacga ccgcgacccg gtcggccgcg tcgagcagct
     1441 ggagccggag ctgcgggtcg cggaggaacg tgttcacgag gtccggggtg tcgtacttct
     1501 gcaggaccgg ccgggtgaac cggccccggg accccccggc tcccgggtcg tcgcgcctcg
     1561 gccagtgcca ccacggggcg accagacgcc agtcgtgccc gaccagggtc tcgacggcgc
     1621 tcaccagacg ttccccgcgc cgggcgtgta gctcggcgac acgacgccgt tcgaggcgtt
     1681 cagggtggtg cacgagatga tcccgtcgaa caccgcggtc gccgcgtgca cgttgagcgc
     1741 ggatgcggtg atctcgacgg tggcgttggc cttgatctcg acctggcccg ccgcgttcag
     1801 ggtgaccgtc ggcccgttca tgtgggtgag ctcgatcttc tgcgtcccct cgtcgagcac
     1861 cagcaggtga ccctgcttcg tcttgatcgt gagtttgggg ccggagggtc cgtcgtcgaa
     1921 ctcgaggagc gtctcggacc gggacttgat gacgcgcttg tcgttcggct tggtcggggt
     1981 gaccggcacc atctccttgc cgttccagca ggagccgacg atgtacggac ggcggacgtc
     2041 ccctgcctcg aaggccacga cgacctgggt gtcgaccgcc gggaacgcga cgaacccctg
     2101 gtcgtcgtcc gcgtacggcg tgagcagcgt cgcccacgcc cgcacctggt cccccgccga
     2161 tccgagccac gggaaccgga cctcgacgcg gccgagccgg tcggggtcga cgatgtcggt
     2221 gacgatcgcg gggtacacgc cgaagaaccg cccggcgtcg ccggcctcgc gcgcgccggt
     2281 cacgtccccg ccccgatcgt cgggcgctcg gccaggaagt gcgtgcggtg gccgcgcgtc
     2341 aggtcgtagg tgtggtggac ccgggtgacg tagtagccgt tgccctcgaa cggcttcccg
     2401 acccgaccga gcttcagccg gctgccgacg accatctcgg gcgagccgtc cgtcgtcccc
     2461 tcgatcgtga cgaagccccg ggcccggcgg agcatctccg cctccgcgaa cgcgcgcgcg
     2521 tcggcgtcca cgaacgggac gagccgggtc tcctggccgg ccatcgcgcc gaacgccttc
     2581 tgcaggacat cgggcccggt tcggccgccg ttcgcctccg cggacaccac cgaggacgcg
     2641 ccctcgacgt cgacccggtc ccggctcttc gcgtcatagc ccgacacgtt gatcccggtg
     2701 cactggtgcg cgaggtcggc ccgcaccgac gcggcgagca ggttgctgcc ctgcgtcagg
     2761 gtcagcgtcg tgccctgccg ctggtcgcgg gtggcgaagt ggagcgtccc gtcggccgcc
     2821 cagagctcgg cgctcacccg cgaggcccgc tcccggagga acgccaggtc gctctggttc
     2881 agctgctgaa cgacgtcgta cgtcgggccg ttcgccgcga tctccggacg caggccgtgc
     2941 ttcccggcga tcgactgcgc gacgtcgccg tcggtgacgt tgcggtaggt ggaggaccgc
     3001 tgcgtcatcc ggagcagcat cagctcgtcc tccgcgaggg ccgtgacgtg ggggacgtcc
     3061 ccctcctcga acgagacctc gagcgcggac accttccctg tgaacacgac cttctcgttg
     3121 ccgggcgggc ccatggacac ctcgaccttc ttgccgaagt cgaggacggt gccgtcgagg
     3181 tactccgcca cgtccgtggt cggcgtcgac ctggcggcgc tcgccacgag gtggagcgtg
     3241 agcgtccgga gcccgcaggt gtcctcctgc acgtcgagcc gcaacaggtc gcgggcgagg
     3301 tcggcaacgc ggttcccgtc gaccttcacg accggcgagg tgctcgcgta gcgggggcgg
     3361 tcagccatcg aatctcctcg cctcggtccg gcactcatcg cggcgcatcg cgcgctcgag
     3421 ggcgaggtgg cgttcgcggt cattccagct cggctggagc ttcccgtccg ggccgcggcc
     3481 ctccggggac ccgtcgccgc ggccggtgac ctggacctcg gaggtcagct cgccgacgcg
     3541 gacgctcacc ggacgcctcc gcacccgcat ccgcaggacg cgaacccgtg gcgggagcgg
     3601 gcggccgcgg ctcccgccgc gggcgcaggc gggagcgcct gccacgacgg gacggtcggg
     3661 tcgtcggtga cgggaacgtc accgacggac gcggcgaccc ggccggggaa ccgcgcgtgg
     3721 acgagcgcga ccgtggcggc cgtcctgccg ccggcgcggg gccggagcgg gacgccgaac
     3781 ccgaacgacg tcgtcctgcc gtcgaccccg ggcaccccgg tggtcgccgc cgccgggacc
     3841 cccgcgggcg ccgccggcgg ggtcgcgggg gtcggggacg cggggaacgc gcgtcgcgcc
     3901 gcgtccgcgg cggcggtggc acgcgtggcc gtcgccctgg cggcggccac gctcaaccca
     3961 ccctcggcgg tgagggcgcc gccgtcggtc gggaccggcg gcgggcccgg tggcgggacg
     4021 gcgcccggcg gcgcggaccc gggcggcgcg gcgggcgcgg ccccaccccc cgggccgccg
     4081 ccgggggatc cccccgcgtc gatgccggcc gtcgcgctca cctcgacccc gagcccgacg
     4141 tccgccgaga gcgacgagga gaagtcgatg agcgcgccgg ccgcgaggct cagggggttg
     4201 gcgatcccgt ccaggccctt ccacgccttc gggtcgaggc ccatgcggtt ggcgaaatcc
     4261 gacgcgctct ccccggcgag cgcggtgccg gtgcggtcgg gtggccgggg tgccgatccc
     4321 gcctgcggcg ggatcggcgc ggtcgcgccg gcgcccgtgt tcgcccccgg cccctgcagg
     4381 ccggcgtcga actcgggctt ctgctccttg atcgtgaccg cgcacttcgc ccggaggggg
     4441 acgccgttct gcgtgaacag gtcgaactcc tggctcatcg ccgacatcac gccgacgacc
     4501 tggagggtgc cgtacttgaa caggacccgg ggcggcacgg ccttcgcgtc cttcttcggg
     4561 agcatgaacc gctcgagctg gcgcgtccgg gtccgcacgt cgacgggcac ctcggtcgtc
     4621 ccctcgtcgg cggtgtcgaa gatcaggtcg aaagaaagcg tcgacgacga cgaaccctga
     4681 tactgggtgt tcggccgcgc gaaggccttg cccatgtcca cgttgttggc gagctggagc
     4741 cgcagcgacg ccgggttgat ctggaccttg atctcctctc cgacggtcgc gggcgtcgga
     4801 gacggcttgc tgtcgacctc ctgaaggctc gccttcttga actccatcag cggatcacca
     4861 tccgcaggcc ctcgtgggcg atctggagct cctcgacggc gacctcgccg gtgcgggcgt
     4921 tcagctgcgg cccgacgatc ttcgcgggga ggccgcgtgc gaacgaccac gtggccaccg
     4981 ggcgctcccc gaccccgagg acgaggaccg tgccgtcgta ccggcggatc gggcggaccc
     5041 catccacgac gtcctggagc cagccccaca gatccgaggc gacggtcccg tcgctcggat
     5101 ggagcatccc ccgcttcagc gtcagacggg tgaacttcgc ccgccccgcg cgctgaatca
     5161 cggcgttgtt ccgcccgccc tcctggatct ccgagacgtc catctcgacg tcgagca
//
LOCUS       DASPWO010000572         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_847871, whole genome shotgun sequence.
ACCESSION   DASPWO010000572 DASPWO010000000
VERSION     DASPWO010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847871"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(556..639)
                     /locus_tag="VEO00_13060"
     tRNA            complement(556..639)
                     /locus_tag="VEO00_13060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(603..605),aa:Leu,seq:cag)
     gene            794..>2031
                     /locus_tag="VEO00_13065"
     CDS             794..>2031
                     /locus_tag="VEO00_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYU58959.1"
                     /translation="MEVVVTLRAPALAQAIAESRVLTASTKGRRLDLGSPTSVSYLRD
                     LDRAQRRVARRIVRTIPSAHVRWRYRVVLDGLAIVLPASRLQALARIPGVVRVWPGTG
                     FHPTLDRSPQLIGADQLWGLPSFSTAGNGVKIGIIDDGIDQKHPFFDPAGYSYPPGFP
                     KGNTSYTTPKVIVARAFAPPTTTWKYARVPFDPVYSDHGTHVAGIAAGDYTPGAVLSR
                     GPLAGVAPKAYLGNYKMNSTPFGDEMIENSAELAAAIEAAVRDGMDVINISYGEIESG
                     SAHNIVDRAVDAAAAAGVVPVVAAGNDFEEFGRGSISSPGTAARAISVAAVSKSDVIA
                     SFSSSGPTPLADRLKPDVAAPGVSILSSVPSREGTWVQFSGTSMATPHVAGGAALLRQ
                     RHPEWSVAQVKSALVLTAGPA"
BASE COUNT          302 a          727 c          679 g          323 t
ORIGIN      
        1 cacacattcg cgacctgggc gatcgagggc gggatgagcc tgatcctgct cgcccggatc
       61 atgggcacga gcgtgcggga gctcgaggac acctacttcc ggtggctcga gcgcaccgat
      121 gagcaggtgc gcgggctgct cgacgagtac gacgcggcgg tttctcagtg atcgaggagg
      181 ctgacctccg ctctgcggtg cccgcggtgt tcggggccga cctgaccatc gtgccctaag
      241 ggcgcaagat caagcaggcg ctcgcggacc tctccagcag cgagagacgc ctagcagttc
      301 tcgtcccggc ccgcacttga tacgcgctgg tggtgggact actgctgccg cagcgaggtc
      361 tgctttctgc ggggccgcgt tcggttcgcg ggcgagcgga gcggcgcgcc ctttccttca
      421 gcagtagtca tcttcgggaa gggagcggac ccgagccggg tgcactggtg cgacgtctac
      481 tccggcgagt tgtgggcggt gccggtgcga aacgtttggg caccgattgg gcaccggcac
      541 cgcgcagctt ggctatgcgg atgagaggac ttgaacctcc acgggctttc gcccacacgg
      601 acctgaaccg tgcgcgtcta ccaattccgc cacatccgcg cggcgtcacc atcttagccg
      661 cgggtttgcc gcatccaccc gcgcgcggcg cacgtaccat tcgccgacct tgagacgggg
      721 tgcgctcctc ctcgcggccg cggccgcgta ttcgcttctg ctcgcgcccg cagggcaggc
      781 gggctccagg cgcatggaag tggtcgtcac gctgcgcgcg cccgcactgg cacaggcgat
      841 cgccgagagc cgggtcctca cggcgagcac caaggggcgc cgcctcgacc tcggctcacc
      901 gacgagcgtc tcctacctcc gcgatctcga ccgggcccag cgcagggtag cgcgacggat
      961 cgtgcgaacg atcccatccg cacacgtccg ctggcgctac cgcgtcgtcc tcgacgggct
     1021 ggcgatcgtc ctgcccgcga gccgcctcca ggcactcgca cggatccccg gcgtcgtccg
     1081 cgtctggcct ggaacgggct tccacccgac gctcgatcgc agcccgcagc tgatcggagc
     1141 cgaccagctc tgggggctgc ccagcttctc gaccgcgggg aacggcgtga agatcgggat
     1201 catcgacgac gggatcgacc agaagcaccc cttcttcgat cccgcgggct acagttatcc
     1261 gccaggcttc cccaagggaa atacgtcgta cacgaccccg aaggtgatcg tcgcccgggc
     1321 gttcgcaccg ccgaccacga cctggaagta cgctcgcgtc cccttcgacc cggtctactc
     1381 cgatcacggg acgcacgtgg ccggcatcgc cgctggtgac tacacgcccg gggctgtctt
     1441 gagccggggg ccgctggcgg gagtcgcgcc gaaggcctat ctgggcaact acaagatgaa
     1501 ttcgacgccg ttcggagacg agatgatcga gaactcggcc gagctcgccg cggcgatcga
     1561 ggccgccgtt cgcgacggga tggacgtgat caacatctcc tacggcgaga tcgagtcggg
     1621 ctcagcccac aacatcgtcg accgtgcggt cgacgcggcc gccgcggcgg gtgtcgtacc
     1681 ggtcgtcgcc gccgggaacg acttcgagga gttcggccga ggctcgatct cttcacccgg
     1741 aaccgcggcg cgcgccatct cggtggccgc cgtgtccaag tccgacgtga tcgcgtcctt
     1801 ctcctcgagc gggccgaccc ccctcgccga ccggctgaag cccgacgttg cggcacccgg
     1861 cgtctcgatc ctctcgtcgg ttccgtcgcg cgagggcacg tgggtccagt tctcggggac
     1921 cagcatggcc acgccccacg tcgcaggcgg cgcggcgctc ctccgccagc gccatcccga
     1981 gtggagcgtt gcccaggtca agtccgcgct cgtcctcacc gcaggccccg c
//
LOCUS       DASPWO010000573         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_251131, whole genome shotgun sequence.
ACCESSION   DASPWO010000573 DASPWO010000000
VERSION     DASPWO010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251131"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VEO00_13070"
     CDS             <1..1154
                     /locus_tag="VEO00_13070"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYU58960.1"
                     /translation="GLVVFTLVIIMVLGAMIDASADGTMADASGGFPIRADFNPSDPV
                     GHPVTAFTTGRFAGRVETVVPLVVAPGLAAVGAIADADVVVVGADYGIVASGLYPLTE
                     RLPLAESDRAAWEMVLRNTRWAIVDQFIGQAGGGPPAVVYHPGDEVTLTDPATGRSET
                     KVIAGILKASDGLRGIQNTGYTSPIVMGTRSAHELFGTLARRASALIKPAPGEPLAAL
                     QADLQGQFLANGLIVTDVRSVVERSLSATRGFFQLMEGFLALGLLVGIAGLGIVMVRA
                     VRERRRTIGVLRALGFGARTVRRAFLGESAFVAFEGIVLGAGLGLVTSYLMFVNFPSF
                     EGMGIGFPVPWTALAVVLVITGVASLLATMWPARRAANIRPAVALRIAD"
     gene            complement(1151..2095)
                     /locus_tag="VEO00_13075"
     CDS             complement(1151..2095)
                     /locus_tag="VEO00_13075"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HYU58961.1"
                     /translation="MENVLLAAPRGYCAGVDRAVDTVELALQRYGAPVYVRKQIVHNL
                     HVVRSLEAKGAIFVEEETEVPEGATVVLSAHGVAPSVYANSEARALNVIDATCPLVTK
                     VHLEARRFARDGFTILLIGHEGHEEVVGTSGEAPDRIVLVGTVDEARSVEVPDPERVA
                     YLSQTTLSVDETNEIIDVLRGRFPGIQGPPRDDICYATQNRQEAVKEIARRSDVVLVI
                     GSRNSSNSKRLVEVAASAGAPAHLVDDETEIDPAWLDGAAVVGVTSGASAPEWLVERV
                     LGWLREHGAPRAEEVHVVEEHMAFSLPAGVRPVAASSS"
     gene            complement(2221..2559)
                     /locus_tag="VEO00_13080"
     CDS             complement(2221..2559)
                     /locus_tag="VEO00_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58962.1"
                     /translation="MCRSLRVLCAAPSPEELAALKRAAVSSDYELVGGATDRGGVVAQ
                     VREWVPDVVVAPAALLGREAIEEARAWRPELRWVVVGGDGDGADAVAAALEEVRETIR
                     GLPRPGGPVR"
     gene            complement(2562..>3061)
                     /locus_tag="VEO00_13085"
     CDS             complement(2562..>3061)
                     /locus_tag="VEO00_13085"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HYU58963.1"
                     /translation="AFDEAVDRLEDRVFAEAVEAGLQHDLFRLKKGLVDFRRFVLPLR
                     DVLDFMQEDTLVVTPPLKPYFRDVYDNVYRAIEFVDNTRETLTIGLGAYQAAVGNQLN
                     VIMKKLTAWAAILLVPTLIAGIYGMNFEHMPEIPTRYGYFVTIGVMLGVCTLLYRSFK
                     KRDWL"
BASE COUNT          431 a         1095 c         1082 g          453 t
ORIGIN      
        1 acggcctggt ggtgttcacg ctggtgatca tcatggtgct cggcgcgatg atcgacgcca
       61 gcgcggacgg gacgatggcg gacgcgtccg gcgggttccc gatccgggcc gacttcaacc
      121 cgtcggaccc ggtcgggcac ccggtcaccg cgttcaccac ggggcggttc gcagggcggg
      181 tcgagacggt cgtcccgctg gtcgtcgccc ccgggctggc cgcggtcggg gcgatcgcgg
      241 acgccgacgt cgtcgtggtc ggcgcggact acggcatcgt cgcctctggg ctctatccgc
      301 tgacggaacg gctgcccctc gcagagagtg accgcgcggc ctgggagatg gtcctgcgga
      361 acacccgatg ggcgatcgtc gaccagttca tcggccaggc cggcggcggg ccgccggcgg
      421 tcgtctacca ccccggtgac gaggtgacgc tgaccgatcc ggcgaccggg cggagcgaga
      481 cgaaggtcat cgccgggatc ctgaaggcgt ccgacggcct gcgcgggatc cagaacaccg
      541 gctacaccag cccgatcgtc atgggcacga ggtcggcgca cgagctgttc ggcacgttgg
      601 cccgccgggc gtcggcgctg atcaagcccg cgccgggcga gccgctcgcc gcgctgcagg
      661 ccgacctgca gggccagttc ctggccaacg ggctgatcgt gacggacgtc cgaagcgtgg
      721 tggagcgcag tctctcggcg acccggggct tcttccagct gatggagggc ttcctggcgc
      781 tcgggctcct ggtcgggatc gccggcctgg ggatcgtgat ggtccgcgcg gtgcgcgagc
      841 gccggcggac gatcggcgtg ctgcgcgctc tggggttcgg cgcgcgaacc gtgcggcggg
      901 cgttcctcgg cgagagcgcc ttcgtcgcgt tcgaggggat cgtgctcggc gcgggcctcg
      961 gcctggtgac cagctacctg atgttcgtga acttcccgag cttcgagggg atgggcatcg
     1021 ggttcccggt gccgtggacg gcgctcgcgg tggtcctggt gatcaccggc gtcgcgtcgc
     1081 tcctggcgac gatgtggccg gcgcgacgcg ccgcgaacat ccggccggcc gtggcgctgc
     1141 ggatcgcgga ctaggaggag gacgccgcga ccggacggac gccggcgggg agcgagaacg
     1201 ccatgtgctc ctcgacgacg tggacctcct cggcccgggg cgcgccatgc tcccgcagcc
     1261 agccgagcac gcgctcgacc agccactccg gggccgacgc acccgacgtg acgccgacca
     1321 cggcggcacc gtcgagccag gcagggtcga tctcggtctc gtcgtcgacg aggtgggcgg
     1381 gggcgccggc cgaggcggcg acctcgacca agcgcttgga gttcgacgag ttgcgcgagc
     1441 cgatcacgag cacgacgtcc gagcggcgag cgatctcctt gacggcttcc tgccggttct
     1501 gcgtcgcgta gcagatgtcg tcgcgcggcg gcccctggat gccggggaag cgaccgcgca
     1561 gaacgtcgat gatctcgttc gtctcgtcga ccgagagcgt ggtctgcgag aggtaggcga
     1621 cgcgctcggg gtcggggacc tccacactgc gcgcctcgtc cacggtgccg accaggacga
     1681 tgcggtccgg cgcctcgcca ctcgtcccga cgacctcctc gtgcccctcg tgcccgatca
     1741 gcaggatcgt gaagccgtcg cgcgcgaagc gccgcgcctc gaggtggacc ttggtcacga
     1801 gggggcaggt cgcgtcgatc acgttcagtg cccgcgcctc cgagttcgcg tagacggacg
     1861 gcgcaacgcc gtgagcggag agcacgacgg tggccccctc gggaacctcg gtctcctcct
     1921 cgacgaagat cgcgcccttc gcctccagcg accgcacgac gtgcaggttg tgcacgatct
     1981 gcttgcgcac gtagaccggc gcgccgtatc gctgcaaggc gagctcgacc gtgtccacgg
     2041 cccggtccac gcccgcgcaa tagccgcgcg gcgcggcgag cagcacgttc tcgatcacga
     2101 gaccatgcta gtccgcgctc aggctgatac gtcttccgcc cgagtcggcc tgcctgcttg
     2161 cttttgcaaa gcattccgga ctcggtccgg cacaccccgc gccatccccg gaccgattcc
     2221 ctatctgacc gggccgccgg gccggggcag gccccggatc gtttcgcgaa cctcctccag
     2281 cgcggcggcg acggcgtcgg ctccgtcacc gtcgccgccg accaccaccc accgcagctc
     2341 cgggcgccac gcgcgcgctt cctcgatcgc ctcgcgcccc agcagggcgg cgggggcgac
     2401 gaccacgtcg gggacccact ccctgacctg ggccaccacg ccgccacgat ccgtggcgcc
     2461 gccgaccagc tcgtagtcgg acgacacggc ggcgcgcttc agcgccgcca gctcctccgg
     2521 cgacggcgcg gcgcacagca cgcgcagcga gcggcacatc gctacaacca gtcgcgcttc
     2581 ttgaacgacc ggtacaggag cgtgcacacc ccgagcatca cgccgatggt gacgaagtac
     2641 ccgtagcgcg tcgggatctc gggcatgtgc tcgaagttca tcccgtagat gccggcgatc
     2701 agcgtcggga ccagcaggat cgccgcccag gccgtcagct tcttcatgat cacgttcagc
     2761 tggttcccga cggctgcctg gtacgcgccc agcccgatcg tcagcgtctc cctggtgttg
     2821 tccacgaact cgatcgcccg gtacacgttg tcgtacacgt cgcggaagta cggcttcagc
     2881 ggcggggtca cgaccagcgt gtcctcctgc atgaagtcga ggacgtcgcg cagcggcagg
     2941 acgaaacggc ggaagtcgac caggcccttc ttcagccgga acagatcgtg ctgcaggccg
     3001 gcctcgaccg cctcggcgaa cacgcgatcc tcgagccggt ccaccgcctc gtcgaacgcc
     3061 t
//
LOCUS       DASPWO010000574         7478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_673107, whole genome shotgun sequence.
ACCESSION   DASPWO010000574 DASPWO010000000
VERSION     DASPWO010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673107"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VEO00_13090"
     CDS             complement(<1..1099)
                     /locus_tag="VEO00_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HYU58964.1"
                     /translation="MIRLRAGLVRAVLDERPGLVELSVEVEGAEARAVAYPDLTGPVA
                     PGDRVQLNTTAVDLALGTGGVHFVLAVEGQRDLDPPPEGHVMKLRYTPLQVKVRAAEE
                     QGGPDREAVAGSAGLAGLPVIWAPLHSMVGAATAGALAAGAERVVHVMTDGGALPAAF
                     SRQLHELRATGLLDAVVTAGQAFGGDHEAVNLFSGLLVAKEVAGAEVVVVADGPGKVG
                     TGTTWGTSDVAGGLALVAAHVLGGRPVATLRLNFADPDYRHYGVSPHSLTVLREVAVI
                     PVHVAVPALDDEERRVVWNALRGAGLEDRHQLVEATGQPALELLEARGVSADTMGWTL
                     AEEPAFFLAAGAAGTLAGRMAVQDRHWRPEPA"
     gene            1149..2090
                     /locus_tag="VEO00_13095"
     CDS             1149..2090
                     /locus_tag="VEO00_13095"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HYU58965.1"
                     /translation="MNPLERLLDLIALLRSRERPVTFEEIRREMPEAYGHGDHESAKR
                     MFERDKEQLREAGLVLEMAATDVWDVEQGYVLRADRTYLRDLAFTPEEAMALFVAATG
                     TGESGEARTAFQKLATGAEGAEIGWLDAAPVGGADASGPHLTALAAAVAGRRAVRFDY
                     RRADGAEDERTLDPWGLVFRGSSWYVIGHDRAREDVRAFRLSRITSDVADAGEGAPPP
                     DGFQAAGHVTTPGASDGGTARVAFAERLAWTVLPSVPAASETGRSGGWARVEVPVAGD
                     EAFIAWVAAFGPDARLLEPERLRAALVAHLERVRDAG"
     gene            2077..3069
                     /locus_tag="VEO00_13100"
     CDS             2077..3069
                     /locus_tag="VEO00_13100"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HYU58966.1"
                     /translation="MTPARASAARARGSSRTGERLRRLLAIVPYVLRHPGTELDELSR
                     LFGVGREDLVRDLNVLLVTGVPPFYPHQMVDVEIDEERVWISAADHLSDPVRLTRDEA
                     LALYLQGKALLGAPGLAESGDLASALAKLESALQESLGGLAGGVEVAAGPSAERERAV
                     LDAVRAAVADRERLAIRYYSASRDEVTNRRIDPEHVFAALGHWYVAAWDPDAGGERLF
                     RLDRIHGTEPAGETFEPRGLAGAGRPLYSRTDADIPVRLVLRPQARWVAEYYEVEDER
                     ERDDGSLEVTLPTKDLAWVAKLLLRLGGRASVLHPPELAEAARRLAEQTLEAYR"
     gene            3112..3405
                     /locus_tag="VEO00_13105"
     CDS             3112..3405
                     /locus_tag="VEO00_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58967.1"
                     /translation="MTTIRTTCPSCGEVDMTPERIVLAIRPEGSAYRFDCPLCEEQVE
                     KRADRKVVALLLSAGVVLEEANGLPPAFTPADALELRSLLDDQGFVDRLTGQR"
     gene            3434..3694
                     /locus_tag="VEO00_13110"
     CDS             3434..3694
                     /locus_tag="VEO00_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58968.1"
                     /translation="MFVAVVFVLVGVISLALIVLVVGLVRHVKVLAAAVARFQREIEP
                     SLARIREDAEAAQERLAATSERAERLRAEAERLGGAGRKRRR"
     gene            3752..4027
                     /gene="tatA"
                     /locus_tag="VEO00_13115"
     CDS             3752..4027
                     /gene="tatA"
                     /locus_tag="VEO00_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HYU58969.1"
                     /translation="MFNLGPGEILVILVLALLIFGPKRLPEIGRSIGKSMREFRRASS
                     ELRDEFENLGEEKTRPAAPRQAPGSTPGGPSGNGPRNEPPGNDDPDG"
     gene            4112..4876
                     /gene="tatC"
                     /locus_tag="VEO00_13120"
     CDS             4112..4876
                     /gene="tatC"
                     /locus_tag="VEO00_13120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HYU58970.1"
                     /translation="MPLIPSRRRRTSAMTVVEHLTELRRRLVIALSAVVVGSIAGWFL
                     FQQTFGLFRDPYCDALAKLPATVRPPQGCALIFTGIPDAFLMRLKLAVFTGFAFALPV
                     VLYELWMFITPGLTKRERRLALPFVLSSVGLFALGAWFAFLTLPKGLQFLLGFGGELV
                     VPFLTVDRYVGFVTILILVFGLSFEFPLLLIFLAGARVITSAQMRRWRRGAWLGSAIA
                     AAVLTPSQDPYTMLAMMVPLIVFYELSVLVARLFKR"
     gene            4927..5613
                     /locus_tag="VEO00_13125"
     CDS             4927..5613
                     /locus_tag="VEO00_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58971.1"
                     /translation="MAIKGKSRSRGGRVGNAPRPVIVIKKMPFLQRRSTRMGALLLVL
                     GVIGATLWIGFAKQRVNEDRARQGESVSRINSQVQNNLQGISQPLPPSNLSILPDLAG
                     NLEQFKSGDLSAKKVRDATRLIPKTMQGAAETIGGIEVPDELRNHDYTADLLDALFLM
                     SQGLKAYGNVAASMLLATNLDGDARADLLDSATDALADGQKLFDHGYQKLTNIRTELG
                     VYSPTSLGAQ"
     gene            5644..6375
                     /locus_tag="VEO00_13130"
     CDS             5644..6375
                     /locus_tag="VEO00_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYU58972.1"
                     /translation="MTTGLVLVHAFPLDASMWKPQTEAFGGRVAGAAPDLPGFGDSPG
                     AGAVMSMDDAADRVLLAIDQMAMDRAVLAGVSMGGYAMFSCWRRFRDRIAGMLLADTK
                     AEADDEAAVERRRALAENVEANGIEVVVDNPPPLLSPNAPPELWDEVKAIIARQSPEA
                     IAAASRGMAARPDSRPILPEIDVPTIVVVGSEDAITPPANAEALAGAIGADLRVIEGA
                     GHISNLEAPDAFNAVLSDLLDRCGL"
     gene            6430..>7478
                     /locus_tag="VEO00_13135"
     CDS             6430..>7478
                     /locus_tag="VEO00_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYU58973.1"
                     /translation="MQDIERFATRYPFPLDEFQVHAIEGLAAGESVLVAAPTGSGKTV
                     VAEYAVERALGADAKAFYTTPLKALSNQKFGDFVARYGAARVGLLTGDNSINGEAPIV
                     VMTTEVLRNMLYEGSDTLEGLRFVVMDEVHYLQDPYRGAVWEEVLIHLPLSVSVVCLS
                     ATVSNAEEFGEWIGTLRGPTRVVIEERRPVPLEHLYLTGGDLYPIHVERDGEPTANPE
                     LVRLERDEARRQRDLERGRYVRRPRRMRVPRREEVVEVLHRHEMLPAIYFVFSRAGCQ
                     RSVQWMLEAGIRLTSPEEEARIREFAEMRAAMIPDEDLDTLGFGEFREALAAGVAAHH
                     AGMLPVFKETVEELF"
BASE COUNT          992 a         2737 c         2716 g         1033 t
ORIGIN      
        1 aggccggttc cggtcgccag tggcggtcct gtacagccat ccggccggcg agcgtcccgg
       61 ccgcgccggc ggccaggaag aaggccggct cctccgccag ggtccagccc atcgtgtccg
      121 cggagacgcc ccgcgcctcg agcagctcga gcgccggctg ccctgtggcc tcgaccagct
      181 ggtgacggtc ctccaggccc gcgccgcgga gcgcgttcca gaccaccctc cgctcctcgt
      241 cgtcgagcgc cggtaccgcg acgtgcacgg ggatcacggc gacctcgcgc agaacggtca
      301 gcgagtgcgg ggacacgccg tagtggcggt agtccgggtc cgcgaagttc aggcgcagcg
      361 tcgccaccgg ccggcccccg aggacgtgcg ccgccacgag cgccaggcca ccggccacgt
      421 cgctcgtccc ccaggtggtg ccggtaccga ccttccccgg gccgtcggcc acgaccacca
      481 cctcggcccc ggccacctcc ttcgccacga gcagccccga gaagaggttc acggcctcgt
      541 ggtccccgcc gaaggcctgg ccggcggtga ccacggcgtc gagcagcccg gtggcccgca
      601 gctcgtggag ctggcgcgag aaggccgccg ggagcgcccc gccgtcggtc atcacgtgca
      661 cgacgcgctc ggcgcccgcg gcgagcgccc cggcggtcgc ggcaccgacc atcgagtgca
      721 ggggcgccca gatcaccggc agcccggcga gcccggcgct cccggccacc gcctcccggt
      781 cgggaccgcc ctgctcctcg gccgcccgga ccttcacctg cagcggcgtg tagcggagct
      841 tcatcacgtg gccttcgggc gggggatcga ggtcgcgctg gccctcgacg gccagcacga
      901 agtgcacgcc gccggtgccg agggcgagat cgaccgcggt ggtgttcagc tggacgcggt
      961 ccccgggcgc cacgggcccg gtcaggtccg ggtaggccac ggcgcgcgcc tcggcgccct
     1021 cgacctccac ggacagctcc accaggccgg ggcgctcgtc cagcacggcc cggaccagcc
     1081 ccgcgcgcag cctgatcacg cgcggatcgt aggggaatcg cggtccggcg cgggcctatc
     1141 atcggccggt gaaccccctc gagcgcctgc tcgacctgat cgcgctgctg cgctcgcgcg
     1201 aacgcccggt gacgttcgag gagatccgcc gcgagatgcc cgaggcctac ggccacggtg
     1261 accacgagag cgcgaagcgg atgttcgagc gcgacaagga gcagctccgg gaggccggcc
     1321 tggtcctgga gatggccgcc accgacgtct gggacgtcga gcagggctac gtcctgcgcg
     1381 ctgaccggac gtacctgcgc gacctggcgt tcacgcccga ggaggccatg gcgctcttcg
     1441 tcgccgccac cgggaccggg gagtccggcg aggcccggac ggccttccag aagctcgcga
     1501 cgggggccga gggggccgag atcggctggc tggacgccgc gccggtcggg ggggcggacg
     1561 cctctggccc gcacctcacc gcgctggccg ccgccgtcgc cggccgccgc gcggtccggt
     1621 tcgactaccg gcgcgcggac ggcgcggagg acgagcgcac cctggacccg tgggggctcg
     1681 tgttccgcgg ttcgtcctgg tacgtcatcg gccacgaccg cgcgcgggag gacgtccgcg
     1741 cgttccgcct ctcgcggatc acctcggacg tggcggacgc cggggagggc gcgccgcccc
     1801 ccgacgggtt ccaggccgcc gggcacgtca ccacgcccgg cgcctccgac ggcggcacgg
     1861 cgcgggtggc cttcgccgag cggctggcct ggaccgtcct cccgtccgtg cccgcggcgt
     1921 cggagaccgg ccggtccgga gggtgggcgc gggtcgaggt gccggtggcc ggtgacgagg
     1981 ccttcatcgc ctgggtggcg gcgttcgggc cggacgcccg cctcctggag cccgagcggc
     2041 tccgggcggc cctcgtggcc cacctggagc gcgtccgtga cgccggctag ggcatccgcg
     2101 gcccgcgcga ggggctcctc ccggaccggc gagcgcctgc gcaggctgct ggccatcgtc
     2161 ccctacgtcc tgcgccaccc ggggaccgag ctcgacgagc tgtcgcgcct gttcggcgtc
     2221 ggccgggagg acctggtccg cgacctgaac gtcctcctgg tcaccggcgt gccgcccttc
     2281 tacccgcacc agatggtcga cgtggagatc gacgaggagc gggtctggat ctccgcggcc
     2341 gaccacctgt ccgacccggt ccgcttgacc cgcgacgagg ccctggcgct ctacctgcag
     2401 gggaaggccc tcctcggggc gccggggctc gccgagtcgg gcgacctggc gtcggcgctc
     2461 gcgaagctcg agtcggcgct gcaggagtcc ctcggggggc tggccggcgg ggtggaggtg
     2521 gccgcgggac caagcgccga gcgggagcgt gcggtgctcg acgccgttcg cgcggcggtc
     2581 gccgaccgtg agcggctcgc catccgctac tacagcgcca gccgggacga ggtgaccaat
     2641 cgacggatcg acccggaaca cgtcttcgcc gccctggggc actggtacgt ggcggcatgg
     2701 gaccccgacg cgggcggcga gcgcctcttc cgcctggatc gcatccacgg gaccgaaccg
     2761 gccggcgaga cgttcgagcc gcgcgggctg gccggcgcgg ggcggccgct ctactcgcgc
     2821 accgacgccg acatcccggt ccgcctggtg ctccgtcccc aggcgcgctg ggtggccgag
     2881 tactacgagg tcgaggacga gcgggagcga gacgacggtt ccctggaggt cacgctcccg
     2941 acgaaggacc tggcctgggt ggccaagctg ctgctccgcc tcggggggcg ggcctccgtg
     3001 ctgcaccctc ccgagctggc ggaggccgct cgacgcctgg cggagcagac cctcgaggcc
     3061 tatcgctgaa ccctctcaac tcgaccgggc gaccggccga tacctacgct gatgaccacc
     3121 atccggacga cctgcccctc ctgcggcgag gtggacatga cgcccgagcg catcgttctg
     3181 gcgatacgcc cggagggttc cgcgtaccgc ttcgactgcc ccctgtgcga ggagcaggtg
     3241 gagaagcggg cggaccgcaa ggtggtggcc ctgctgctgt cggcgggggt cgtcctcgag
     3301 gaagcgaacg gcctgccgcc ggccttcacg ccggccgacg cgctcgagct gcgctcgctg
     3361 ctggacgacc aggggttcgt cgaccgcctg accgggcagc gctagcctcc ggcggctggg
     3421 gtacccttca gccgtgttcg tcgccgtcgt cttcgtgctg gtcggcgtga tctcgctggc
     3481 cctgatcgtg ctcgtggtcg gcctggtccg ccacgtcaag gtgctggcgg ccgccgtggc
     3541 ccgtttccag cgcgagatcg agccatcgct cgcccggatc cgcgaggacg ccgaggccgc
     3601 ccaggagcgg ctcgccgcca ccagcgagcg ggcggagcgc ctgcgcgccg aggcggagcg
     3661 gctggggggc gccggacgga agcgccgccg ctagggtttg aggtggacac ggggggcgga
     3721 gccccccgtg gtggctagaa tcgagggtgc cgtgttcaac ctcggaccgg gcgagatcct
     3781 cgtcatcctc gtcctcgcgc tcctcatctt cggcccgaag cgcctgcccg agatcgggag
     3841 gtcgatcggc aagagcatgc gtgagttccg ccgcgcctcg agcgagctgc gcgacgagtt
     3901 cgagaacctc ggcgaggaga agacgcgccc ggcggctccc cgccaggccc ccgggtccac
     3961 tcccggcggc ccgtccggca acggcccccg gaacgagccc ccggggaacg acgacccgga
     4021 cggctagttc ggcccgtctc ccttgggggg ttcgctcgcc ggacgtgcat gactctcgcg
     4081 ctccacaggg cgagcaaccc caaacccccc cgtgccgctg atcccctccc gccgccgccg
     4141 gacctcggcg atgacggtgg tcgagcacct caccgagctg cgccgccgcc tggtgatcgc
     4201 gctctccgcg gtcgtcgtgg gctcgatcgc cggctggttc ctgttccaac agaccttcgg
     4261 gctgttccgg gacccgtact gcgacgcgct ggcgaagctg ccggccacgg tccgcccacc
     4321 gcaggggtgc gcgctgatct tcaccggcat ccccgacgcg ttcctgatgc gactgaagct
     4381 cgccgtgttc accgggttcg ccttcgccct gccggtggtg ctctacgagc tgtggatgtt
     4441 catcaccccg gggctgacca agcgcgagcg gcggctggcc cttccgttcg tgctgtcctc
     4501 cgttgggctg ttcgccctgg gcgcgtggtt cgccttcctg accctgccca aggggctgca
     4561 gttcctgctc gggttcggcg gcgagctggt ggtcccgttc ctgaccgtcg accgctacgt
     4621 cgggttcgtg acgatcctga tcctcgtctt cgggctgtcg ttcgagttcc ccctgctgct
     4681 gatcttcctg gccggcgcgc gggtgatcac cagcgcccag atgcggcggt ggcggcgggg
     4741 ggcgtggctc gggtcggcga tcgccgcggc cgtgctcacc ccgagccagg acccctacac
     4801 catgctcgcg atgatggtcc cgttgatagt cttctacgag ctttcggtgc tcgtcgcccg
     4861 actgttcaag cgctgaccgg cgggcgaccg gccacggata tcgggatcgg agaggaagaa
     4921 cgaggcatgg cgatcaaggg caagagcagg tcgcggggcg gccgtgtcgg gaacgccccc
     4981 cggccggtga tcgtgatcaa gaagatgccg ttcctgcagc ggcggagcac ccgaatgggg
     5041 gctctgctcc tggtgctcgg ggtgatcggg gccacgctct ggatcggctt cgccaagcag
     5101 cgggtcaacg aggaccgcgc ccggcagggg gagtcggtct cacggatcaa cagccaggtg
     5161 cagaacaacc tgcaggggat cagtcagccc ctcccgccga gcaacctctc gatcctgccc
     5221 gacctggcgg ggaacctgga gcagttcaag agcggcgacc tgtccgccaa gaaggtccgg
     5281 gacgcgacgc ggctcatccc gaagacgatg cagggcgccg cggagacgat cggcggcatc
     5341 gaggttcccg acgagctgcg caaccacgac tacacggccg acctgctcga cgcgctgttc
     5401 ctgatgtccc aggggctgaa ggcgtacggg aacgtcgccg cctccatgct cctggccacg
     5461 aacctcgatg gtgacgcccg cgccgacctg ctcgactcgg ccaccgacgc gctcgccgac
     5521 ggccagaagc tcttcgacca cgggtaccag aagctcacca acatccggac ggagctcggc
     5581 gtctacagcc cgacctcgct cggggctcag taggcccggg agcctggaga caggagagac
     5641 gacatgacca ccggcctcgt gctcgtgcac gccttcccgc tcgacgcctc gatgtggaag
     5701 ccgcagaccg aggccttcgg cgggcgggtc gccggcgcgg cccccgacct gccggggttc
     5761 ggcgacagcc cgggcgccgg cgcggtgatg tcgatggacg acgccgcgga ccgggtcctg
     5821 cttgccattg accagatggc gatggaccgg gcggtgctcg ccggcgtctc catgggcggc
     5881 tacgcgatgt tctcctgctg gcggaggttc cgcgaccgca tcgccgggat gctgctggcc
     5941 gacacgaagg ccgaggccga cgacgaggcg gccgtcgagc gacgccgggc cctcgcggag
     6001 aacgtcgaag cgaacgggat cgaggtggtg gtggacaacc ccccgccgct gctctcgccg
     6061 aacgccccgc cggagctgtg ggacgaggtc aaggcgatca tcgcccgcca gtcgcccgag
     6121 gccatcgccg cggcctcgcg cgggatggcc gcccgccccg actcccggcc gatcctgccg
     6181 gagatcgatg tcccgacgat cgtcgtcgtc gggagcgagg acgccatcac cccgccggcg
     6241 aacgccgagg cgctggccgg cgcgatcggc gccgacctac gggtgatcga gggcgccggc
     6301 cacatctcca acctcgaagc ccccgacgcc ttcaacgccg tcctgtccga cctcctggac
     6361 cgctgcggcc tgtaggggcc gtcgggaacc gcacgccggc ggttcggctc gaccgcctac
     6421 gctagggacg tgcaggacat cgagcggttc gccacgcgct atccgttccc actcgacgag
     6481 ttccaggtcc acgcgatcga ggggctcgcg gccggagagt ccgtgctcgt ggccgccccg
     6541 accgggtcgg ggaagaccgt cgtcgccgag tacgccgtgg agcgggccct cggcgccgac
     6601 gccaaggcct tctacacgac gcccctgaag gcgctctcga accagaagtt cggggacttc
     6661 gtggcccgct acggtgccgc ccgtgttggc ctcctgaccg gcgacaacag catcaacggc
     6721 gaggccccga tcgtggtgat gaccaccgag gtgctgcgca acatgctcta cgagggcagc
     6781 gacaccctcg agggcctgcg cttcgtggtc atggacgagg tccattacct gcaggacccg
     6841 taccgcgggg cggtatggga ggaggtcctg atccacctcc cgctctcggt gtcggtggtg
     6901 tgcctctcag cgaccgtgtc gaacgccgag gagttcgggg agtggatcgg caccctgcgc
     6961 ggaccgaccc gggtggtgat cgaggagcgc cgtccggtcc cgctggagca cctgtacctg
     7021 accgggggcg acctgtaccc gatccacgtg gagcgcgacg gcgagccgac cgccaacccg
     7081 gagctggtca ggctggagcg cgacgaggcc cggcggcaac gcgacctgga gcgtggccgc
     7141 tacgtccgcc ggccgcggcg gatgcgcgtg ccccggcgcg aggaggtcgt cgaggtcctg
     7201 caccggcacg agatgctccc ggcgatctac ttcgtcttca gccgggccgg ctgccagcgc
     7261 agcgtccagt ggatgctcga ggccgggatc cgcctgacgt cccccgagga ggaggcgcgg
     7321 atccgcgagt tcgcggagat gcgggcggcg atgatcccgg acgaggacct cgacaccctc
     7381 ggcttcggcg agttccggga ggcgctcgcc gccggggtgg ccgcgcacca cgccggcatg
     7441 ctcccggttt tcaaggagac cgtcgaggag ctgttcca
//
LOCUS       DASPWO010000575         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_421691, whole genome shotgun sequence.
ACCESSION   DASPWO010000575 DASPWO010000000
VERSION     DASPWO010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421691"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..509
                     /gene="rplS"
                     /locus_tag="VEO00_13140"
     CDS             96..509
                     /gene="rplS"
                     /locus_tag="VEO00_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HYU58974.1"
                     /translation="MNKTDLVESARLRDDIPDFKPGDTVKVHVRVAEAGRERIQVFQG
                     VVLARKGGGLRETFTVRKVSFGVGVERTFPLHTPSIARIDVMTRGRVRRAKLYYLRER
                     RGKAARIRELREAPRPAAAAAEPGDQGDEASEEQG"
     gene            513..1283
                     /gene="lepB"
                     /locus_tag="VEO00_13145"
     CDS             513..1283
                     /gene="lepB"
                     /locus_tag="VEO00_13145"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYU58975.1"
                     /translation="MSDAGSPTEPQERARARAEARTLTAAPDTETERIPPHGEPPGEG
                     RAGDGEKRKPEEPKGPFSFLRELPVLILVAFVLALLIKTFLVQAFYIPSESMLPTLRV
                     GDRVLVNKVVYHVHPPRRGDIIVFAEPNPVNEPDRTWWQSFTHWLTDGLGVTQDPTKD
                     YIKRVIGLPGEEVEVKRGTVLVDGAALHEDYLSDIEDLSDFGPHTVPDDAYFVMGDNR
                     TNSQDSRSLLGDIPADRIIGKAFVVIWPPSNLSWLPRG"
     gene            1265..1996
                     /locus_tag="VEO00_13150"
     CDS             1265..1996
                     /locus_tag="VEO00_13150"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HYU58976.1"
                     /translation="MATARLNGNGPDETPGPKDLDHYERQLRAQGFRLVAGVDEVGRG
                     ALAGPLVAAAVILPEGFDLEGIDDSKALTAEQREEQCVRIVEGAVAYALCKAMPTRID
                     HRGLHRSNLFLLKRAVRALPVGPDYVLADGLPLRGFPLPHLSIKKGDAVTASVAAASI
                     VAKVTRDRMMDRYHRRYPAYGFDTNRGYGTPGHRRVLRELGPTPIHRHSFVGVASGHY
                     PNVAGIELRVRWDLRGPPAPPSAEG"
     gene            2022..2363
                     /locus_tag="VEO00_13155"
     CDS             2022..2363
                     /locus_tag="VEO00_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005187289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HYU58977.1"
                     /translation="MSAEELEKFETEMELQLYREYRDVLPMFSHVVETERRFYLANQV
                     DVARHEEGDEVWIELVLTDAWVWDMYRSARFVSKVRVFTTRDVNIEELSHKGEEPRVP
                     DTPPDVPGFPG"
BASE COUNT          386 a          916 c          965 g          357 t
ORIGIN      
        1 aaccgccccg acctgcaggc gaaggaccat cggccgcggg gaacggaagg ccccctgcta
       61 tgatgagcct ccccggagcg gcggaacgcc taccaatgaa caagaccgat ctcgtcgaga
      121 gcgcgcgcct gcgcgatgac atcccggact tcaagcctgg cgacaccgtc aaggtgcacg
      181 tgcgcgtggc cgaggcgggg cgggagcgga tccaggtgtt ccagggcgtg gtgctcgccc
      241 gcaagggtgg cgggctgcgc gagacgttca ccgtacgcaa ggtgtcgttc ggcgtcgggg
      301 tcgagcggac gttcccgctg cacacgccgt cgatcgcgag gatcgacgtc atgaccaggg
      361 gccgcgtccg ccgggccaag ctctactacc tgcgcgaacg gcgggggaag gccgcccgca
      421 ttcgcgagct gcgcgaggcg ccgaggccgg ccgccgccgc ggccgagccg ggcgaccagg
      481 gcgacgaggc gagcgaggag cagggctagg ccgtgtccga cgccggctcc ccgaccgaac
      541 cgcaggagcg ggcgcgcgcg cgggccgagg cccggacgct gaccgccgct cccgacaccg
      601 agaccgagcg gatcccaccg cacggcgagc cgccgggcga gggccgcgcc ggggacggcg
      661 agaagcgcaa gccggaggag ccgaagggcc cgttctcatt cctgcgcgag ctgccggtcc
      721 tgatcctggt cgcgttcgtc ctggcgctct tgatcaagac gttcctggtg caggccttct
      781 acatcccgtc ggagtccatg cttcccacac tgcgggtcgg ggaccgcgtg cttgtgaaca
      841 aggtcgtcta ccacgtccac ccgccgcgcc gcggggacat catcgtgttc gccgagccca
      901 acccggtgaa cgaacccgac cgcacctggt ggcagtcgtt cacccactgg ctgaccgacg
      961 gcctcggcgt gacccaggac ccgaccaagg actacatcaa gcgggtgatc gggctccccg
     1021 gcgaggaggt ggaggtcaag cgggggaccg tcctcgtcga cggcgcggcc ctgcacgagg
     1081 actacctgtc ggacatcgag gatctctcgg acttcggccc gcacaccgtg cccgacgacg
     1141 cctacttcgt gatgggcgac aaccgcacca actcacagga ttcccggtcg ctcctcggcg
     1201 acatccccgc cgatcggatc atcgggaagg ccttcgtggt gatctggccc ccgtccaatc
     1261 tgtcgtggct accgcgcggc tgaacgggaa cggcccggac gagacgcccg gccccaagga
     1321 tctcgaccac tacgagcgcc agctccgggc gcagggcttc cggctggtcg ccggcgtcga
     1381 cgaggtcggc cgcggggcgc tcgccgggcc gctcgtcgcg gcggcggtga tcctgccgga
     1441 ggggttcgac ctggagggga tcgacgactc gaaggcgctc acggcggagc agcgcgagga
     1501 gcaatgcgtt cggatcgtcg agggcgcggt ggcctacgcc ctgtgcaagg ccatgcccac
     1561 ccggatcgac caccgcggcc tgcaccgctc gaacctgttc ctgctgaagc gggcggtccg
     1621 ggcgctgccg gtcggtcccg actacgtcct ggccgacggc ctcccgctac ggggcttccc
     1681 gctgccgcac ctgtcgatca agaaggggga cgcggtgacg gcatcggtgg cggccgcctc
     1741 gatcgtggcg aaggtcaccc gcgaccggat gatggaccgc taccaccgcc ggtacccggc
     1801 ctacgggttc gacacgaacc gcgggtacgg cacgcccggg caccggcggg tgttgcgcga
     1861 gctgggcccg acgccgatcc accgtcactc gttcgtcggc gtggcgagcg gccattaccc
     1921 caacgtggcc ggcatcgagc tgcgggtgcg ctgggacctg cggggaccgc cggcgccccc
     1981 ctcggcggag ggataatggg cccggacgag gagggccgag catgagcgcc gaggagctcg
     2041 agaagttcga gaccgagatg gagctgcagc tgtaccggga gtaccgggac gtgctgccga
     2101 tgttctccca cgtggtcgag accgagcgcc gcttctacct ggcgaaccag gtggacgtgg
     2161 ctcgccacga ggagggcgac gaggtctgga tcgagctcgt cctgaccgac gcctgggtct
     2221 gggacatgta ccgctcggcg cgcttcgtct ccaaggtccg cgtgttcacc acccgcgacg
     2281 tgaacatcga ggagctgtcc cacaagggcg aggagccgcg cgtgccggac acgccgcccg
     2341 acgtgcccgg cttcccgggc tgagatcgac cgggcgcccg gcctgtccca gggcttggcc
     2401 gggctcttcc tgctggtggg cggcccgctg tcgacgcggt gccggtggcg caggtcccac
     2461 gttccggccg cctgaccgcg ccagcgccgt tcgtgtcggt ggcggtcgct agcgtgcgcc
     2521 cgtgcgcggg atggcggacg gacgggcgga tctcgggcgg ctcggcgagc gggcggcgtt
     2581 cgcctggtac gccgcgcgcg ggttccggct ggtgcggcgt aact
//
LOCUS       DASPWO010000576         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_338566, whole genome shotgun sequence.
ACCESSION   DASPWO010000576 DASPWO010000000
VERSION     DASPWO010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338566"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VEO00_13160"
     CDS             complement(<1..495)
                     /locus_tag="VEO00_13160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58978.1"
                     /translation="MAIDPSGRILITGFAGFDIVGFVARLEPDGDLDPTFATAGDDGI
                     YFFDEAFPTAETRALDLVRQSNGAIVAVGSIVQGLASQGDGFGIEAWLVLRLTDGGAL
                     DGTFNPGGSPPGTRLLFQGSAFAEARAVALQGNKLIVVGIFIEEACCDGPGANGLITA
                     QFFAP"
     gene            complement(599..1062)
                     /locus_tag="VEO00_13165"
                     /pseudo
     CDS             complement(599..1062)
                     /locus_tag="VEO00_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1141..2226)
                     /locus_tag="VEO00_13170"
     CDS             complement(1141..2226)
                     /locus_tag="VEO00_13170"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYU58979.1"
                     /translation="MTKARASRGRHRPGAPRRARAGRIALGTLIVLALLGSGTAFAAY
                     RYDRASANKVLPGVTVYGVDVGGMTRGEAIAAVQARVDGTLDRTIAVQAGKKETTVTP
                     RDLNAGADVAAAVDRALSLSEGYSWTTRVYHRLWNKPVGQALDVPLAFDQAQIDAFVR
                     GVAGQVAVAPRDASLDWLDGGLVRQKARTGLAVRQARGAKLVLAALQQGSETVTLPLR
                     QIEADVTEENLGSTIIIRISENRLYLYDGLDLARTYDVATGSGGYPTPQGHWEIVNKR
                     LNPTWVNGAKDSWGADLPDFIPPGPGNPLGTRALDLSAPGIRIHGTYADYSIGTYASH
                     GCIRMHIPDSEELFGLVDVGTPVIIVS"
     gene            complement(2276..>2995)
                     /locus_tag="VEO00_13175"
     CDS             complement(2276..>2995)
                     /locus_tag="VEO00_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58980.1"
                     /translation="EASLGAIVVGEVGSSRAGGLRAVAGDPTTAERWILPDAGGGGET
                     VLSVANPATERSAVSARTQTADAQTLLTEITDVSVEGASAQAFGALTPEEAPSSIVIE
                     ATNRVPVFTSRRSGGPQGNGDSAAGSAVGDPQGSWLVPPAAPPGGGHQFLVLENPGED
                     DVAVTIRLFGRAAGDLGPVDVPGGRTIAVPLEDVVGTTPVWALAAAADGTFVAASASY
                     EVDGRGYAVASGVPYRSGIDE"
BASE COUNT          402 a         1099 c         1084 g          410 t
ORIGIN      
        1 cggcgcgaag aactgcgcgg tgatgaggcc gttcgcgccg gggccatcgc agcaggcctc
       61 ctcgatgaag atgccgacca cgatgagctt gttcccctgc agcgcgaccg cccgcgcctc
      121 ggcgaaggcg ctgccctgga agagcagcct cgtgcccggg ggcgacccgc cggggttgaa
      181 cgtcccgtcg agcgccccgc cgtcggtcag tcggagcacg agccacgcct cgatcccgaa
      241 gccgtccccc tgggacgcga ggccctggac gatgctcccc acggccacga tcgccccgtt
      301 cgactgccgg acgaggtcga gcgcccgcgt ctccgcggtc gggaacgcct cgtcgaagaa
      361 gtagatcccg tcgtctccgg cggtggcgaa cgtggggtcg aggtcgccgt cgggctcgag
      421 ccgggcgacg aagccgacga tgtcgaaccc ggcgaagccg gtgatcagga tgcggccgga
      481 ggggtcgatg gccacgccgg tcgcctggct gaactcgctg ccgaggtcga agatggcgat
      541 ccccggcgtc gccccaccgg ggttgaacgt cgcgtcgagg gtcccggtcg ggagcagccg
      601 cgcgacggcg gtctcgtccc cgaaccatcc gaccacgacg gcgttcccgt tggcgtcgag
      661 ggccacgtcg ctcgcgacgt cgcactgtgc cggctcaccc agccggacct tgccgttgcc
      721 gagcgtcccg taggtgccgt ccaggacgcc gtcctcacgg taccgaacga tggcccagtc
      781 gcagttgccg ccgcccgagg cgcggagccc cgatcctcgc ggggacgaga gcgacttgcc
      841 gccgaagaac gcgtcggtgg ccccgacggc gatcaccttc tggtcggcct ggacggccac
      901 ggcgttcgcg ccgtccgacg cgtcgccata ggaggtcttc accttcccgt ccgcactcca
      961 gttcgggtcg aggtcgccgg ggtccgccag cgcggacggg gcggcaaaca gtgacagcag
     1021 gacgagcagc gcgaatgtcg cggtcgggat gcgacgagcc attccggttc ccctttcgat
     1081 caggcatgcg aaccgtacgc acccggtgcg ggcgggtcaa ggtgtcggcg agtgggcggg
     1141 tcaggagacg atgatcaccg gggtcccgac gtcgacgagg ccgaacagct cctcggagtc
     1201 ggggatgtgc atccggatgc agccgtgcga tgcgtacgtt ccgatcgagt agtcggcgta
     1261 ggtgccgtgg atgcggatgc cgggggcgct caggtcgagg gcgcgggtcc cgagcgggtt
     1321 gcccggcccc ggcgggatga agtccggcag gtccgcgccc caggagtcct tggccccgtt
     1381 gacccaggtc gggttcaacc gcttgttgac gatctcccag tggccctggg gtgtcggata
     1441 gccgccgctc ccggtggcga cgtcgtacgt ccgggccagg tcgagcccgt cgtagaggta
     1501 cagcctgttc tcggagatgc ggatgatgat cgtcgagccg aggttctcct cggtgacgtc
     1561 ggcctcgatc tggcgaagcg ggagggtgac cgtctcggag ccctgttgga gcgcggcgag
     1621 caccagcttc gcgccgcgcg cctgccggac ggcgaggccg gtgcgtgcct tctgccggac
     1681 gagccctccg tccagccagt cgagggaggc gtcacgcggc gcgaccgcca cctggccggc
     1741 caccccccgc acgaacgcgt cgatctgagc ctggtcgaag gcgagcggca cgtcgagcgc
     1801 ctgcccgacc ggcttgttcc acagccggtg gtacacgcgg gtggtccagg agtacccctc
     1861 ggagagcgac agggcgcggt cgacggcggc cgcgacgtcg gcgccggcgt tcaggtcgcg
     1921 cggcgtcacg gtggtctcct tcttccccgc ctggacggcg atggtccggt cgagggtccc
     1981 gtcgacgcgg gcctggacgg ccgcgatggc ctcgccacgc gtcatccctc ccacgtccac
     2041 gccgtagacc gtcacgccgg gaaggacctt gttcgccgac gcccggtcgt agcggtacgc
     2101 cgcgaaggcc gtccccgagc cgagcagggc gagcacgatc agcgtgccga gcgcgatacg
     2161 tccggcccgg gcgcgccggg gggcgcccgg acgatgccgt cccctggagg ctcttgcctt
     2221 cgtcacgatc gcatcatacc gagcgttgcc gacacgtcac ccgctcgaac accgctcact
     2281 cgtcgatccc cgatcggtac ggaacgccgg acgccacggc gtagccgcgg ccgtccacct
     2341 cgtaggacgc cgaggcggcg acgaaggttc cgtccgccgc ggcagccagg gcccacaccg
     2401 gggtggtccc gacgacgtcc tcgaggggca cggcgatcgt ccggcccccg gggacgtcca
     2461 ccgggccgag gtcgccggcc gcccggccga acagccggat cgtcaccgcg acgtcgtcct
     2521 cgcccgggtt ctccagcacc aggaactggt gcccgccccc cggcggggcg gcgggcggga
     2581 cgagccagga gccctgcggg tcacccaccg ccgacccggc cgcgctgtcg ccgttgccct
     2641 gcgggccgcc cgaccggcgc gacgtgaaga ccgggacgcg gttcgtcgcc tcgatcacga
     2701 tcgacgacgg cgcctcctcg ggcgtcagcg cgccgaacgc ctgcgccgag gccccctcga
     2761 cgctgacgtc cgtgatctcg gtgagcagcg tctgggcgtc cgccgtctgc gtccgggcgc
     2821 tgaccgcgga ccgctcggtg gccgggttcg ccaccgagag caccgtctct cccccgccgc
     2881 cggcgtcggg gaggatccag cgctccgccg tggtcggatc gcccgccacc gcccgcagcc
     2941 cgccggctcg gctcgagccg acctccccga ccacgatcgc gccgaggctc gcctc
//
LOCUS       DASPWO010000577         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1265197, whole genome shotgun sequence.
ACCESSION   DASPWO010000577 DASPWO010000000
VERSION     DASPWO010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265197"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VEO00_13180"
     CDS             <1..651
                     /locus_tag="VEO00_13180"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HYU58981.1"
                     /translation="PEARRAAAGPRRITHNCSGKHAGMLLACERSGAELAAYLEPSHP
                     LQREILRAVRSATGVQRPAVGVDGCGAPVHGLPLRGMATLFARLARPERLGRLGERAA
                     RAVAAMRSAPYLVAGRGRSDTRLMEAVPGLVCKVGAEGLHCAALLDAGIGVAVRIDDG
                     SDRAAAPALVRTLDLLGVLDEGRRARLASVAAPPVLGGGRPVGRFEVGFRLRGRSG"
     gene            696..2000
                     /gene="mshA"
                     /locus_tag="VEO00_13185"
     CDS             696..2000
                     /gene="mshA"
                     /locus_tag="VEO00_13185"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017596120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HYU58982.1"
                     /translation="MGNGRGTIERVGNGRPATGDDAPRIAVVSLHTSPLDQPGSGDSG
                     GMNVFIRAAAERLSAGGLQVDVFTRSRDERRPEVEELGNGSRLINVQAGPIAPVPKDE
                     LPRFLPEFLGGVLRRARAEGSDYDLVHTHYWLSGWVGRSAREIWGVPLVASFHTLGKV
                     KNYSLSRGERPEPPDRLSGEELVIADADRLVAATPVEAAQLVGLYRAEPDRIRIVPPG
                     VDHGLFFPRDRSRAKARLHLADLRLLLFVGRLQAHKGPDVAIRAVAEAIARDPAGTRD
                     VVLAIVGGPSGTDHGAEVARLMDLASALGVGERVILFPPQPQSRLADFYAAAEAVLVP
                     SRSESFGLVALEAQACSTPVVASAVGGLRYVVEDGVTGHLVEGHDPGDHAERILAVLG
                     DPGERRRMGEAGVARSLRFSWDATAAGMRSVYRELIDGRSLA"
BASE COUNT          292 a          869 c          895 g          370 t
ORIGIN      
        1 cccgaggcgc gtcgggcggc cgccggtccc cgccggatca cccacaactg ctcgggcaag
       61 cacgccggca tgctcctcgc gtgcgagcgc tcgggcgccg agctcgccgc ctatctcgaa
      121 ccgtcccacc cgctccagcg cgagatcctg cgcgcggtgc gttcggccac gggggtccaa
      181 cgaccggcgg tcggggtcga cgggtgcggc gccccggtcc acgggctgcc cctgcgcggc
      241 atggcgacgc tgttcgcacg gctcgcgagg cccgagcggc tcggccgact cggcgagcgc
      301 gccgcccggg ccgtcgcggc gatgcgatcg gccccgtacc tcgtcgcggg gcgcggccgt
      361 tcggatacac ggttgatgga ggcggtcccc ggcctcgttt gcaaggtggg ggccgagggc
      421 ctccactgcg cggccctcct cgacgccggc atcggcgtcg ccgtgcggat cgatgacggg
      481 agcgaccgcg ccgccgcgcc agcgttggtc cggaccctcg acctgctcgg cgtcctggac
      541 gagggacggc gggcccggct ggcgtccgta gcggcgcctc ccgtgctcgg tggcggccgg
      601 cccgtcggtc ggttcgaggt cgggttccgg ctgcgcggcc gatccggctg agcgccttcg
      661 gctgcgttcg gagcgccctc ccgtacgatt cctgcatggg caacggtcga ggcaccatcg
      721 agcgcgtggg gaacgggcgc ccggcgaccg gcgacgacgc gccgaggatc gccgtcgtca
      781 gtctgcacac gtccccgctc gaccagccgg gttcgggcga ctccggcggg atgaacgtct
      841 tcatccgggc ggcggccgaa cgcctgtcgg cgggcggcct ccaggtggac gtcttcacgc
      901 gctcgcgaga cgagcgtcgt cccgaggtcg aggagctcgg gaacggctcc cggctgatca
      961 acgtgcaggc cggaccgatc gccccggtgc cgaaggacga gctcccgagg ttcctgcccg
     1021 agttcctggg gggtgtgctc cggcgggctc gggcggaggg gtcggactac gacctcgtcc
     1081 acacgcatta ctggctctct ggctgggtgg ggcgcagcgc ccgcgagatc tggggggtcc
     1141 cgctggtggc gtcgttccac accctcggca aggtgaagaa ctactccctg tcgcgcggcg
     1201 agcgccccga gcctccggac cgtctgtcgg gcgaggagct ggtgatcgcg gatgcggacc
     1261 ggctcgtcgc ggccaccccg gtcgaggcgg cgcagctcgt cggcctgtac cgggccgaac
     1321 ccgatcgcat ccgcatcgtc cctccgggcg tcgatcacgg cctgttcttc ccgcgagatc
     1381 ggagccgggc caaggcgcgg ctccacctcg cggatctacg gctgctgctg ttcgtgggac
     1441 gcctgcaggc gcacaagggc cccgacgtcg ccatccgggc ggtcgccgag gcgatcgccc
     1501 gtgacccggc tgggacgcgc gacgtcgtgc tcgccatcgt cggcggtccg agcggcaccg
     1561 accacggcgc cgaggtggcc cggctgatgg atctcgcgtc cgccctcggc gtgggggagc
     1621 gcgtgatctt gttcccgccc caaccgcaat cgcgcctggc ggatttctac gcggccgccg
     1681 aggcggtcct ggtcccctcg aggtcggagt cgttcgggct cgtggcgctc gaggcgcagg
     1741 cctgctcgac gcccgtggtg gcgtcggcgg tcggcggtct ccgttacgtc gtcgaggacg
     1801 gcgtcaccgg ccacctcgtc gaaggccacg accccggcga ccacgcggag cggatcctcg
     1861 cggtcctcgg cgatccgggc gagcgccgcc ggatgggcga ggccggcgtc gctcgctcac
     1921 tccgtttctc atgggacgcc accgcggcgg gtatgcgctc ggtgtatcga gagctgatcg
     1981 acggccgcag cctcgcctga accagagcgg cgagcccgtc gcgcgctcga accgttcggt
     2041 cgcgtcgtga gttcgcgcgt ccgcgcctcg gttcggggtc ggaagtccac gccgatgcgt
     2101 atcgtggagt gctctgcacc gcaaggcttt cgaccgatgc gtccacggac gttcgcgctc
     2161 gggacatgtt cttgtcactc tttccatcat gcgatctacc tgcgcatatg taccgtgacc
     2221 agcgcgttcg tcgggttgac ccgccctacc ggcgccggta catttcgctc gctccgagca
     2281 gccggagcgg ccgaatcagg gaaccgaggc cgggacccct gggaaagccg taaggccgac
     2341 tgggcgcacc tgctcgtacg gttgggacag ctaactagct gtgcgggtgg ggaggctcgg
     2401 gaggccccaa agcccaacga aagggt
//
LOCUS       DASPWO010000578         3685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_673598, whole genome shotgun sequence.
ACCESSION   DASPWO010000578 DASPWO010000000
VERSION     DASPWO010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673598"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2410
                     /locus_tag="VEO00_13190"
     CDS             <1..2410
                     /locus_tag="VEO00_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HYU58983.1"
                     /translation="ALLLVAAAAVSGFVLGERADAVAIVAIVLLNAVLGVVEEGRSER
                     ALESLRALEAPAARVVRGGSVVSVPSREVVPGDLVLLAAGDGVPADLGLLEADALEAD
                     ESLLTGESLPVAKRAAVSRAGAPASMAEVPEEARAFSGTLVTRGSGAGRVLATGMATA
                     VGAIAQHVRAPSPPTPLQVEMRRLTATLGTICIVVAAAVLGLTLLRAGSGGIPLDQAF
                     LAAVALAVAAVPEGLATVVTVALALGVRRMASRGAIVRRLPAVETLGATDLILSDKTG
                     TLTENRMRVEAVALPGRAPVPPEALDTPAAERIAEVAVLCNDATLDPPSGDPLEVALL
                     EMAGQERVAALRQSNPRLAVEPFDADRRRMSALHRTTAGSFLFVKGSPEGVLERCSGE
                     TLADGSRVELGPEAREAVLDAAADLAASGMRVLAFAGRSLASAPPDVGAAVQDLDLIG
                     LVALRDPLRPEASRTVSLAASAGVRIVMVTGDHPHTARAIAEAAGVAGRGAAILTGDD
                     LRGAGIPEDLLAAPVYARVSPDQKLALVEAARAGGHVVAVTGDGVNDAPALRRADIGV
                     AMGRSGSDVARQAADMVVTDDNLATIVDAVREGRGIYDNVRSVVDYLVAGNLSEIAVV
                     VTCLLLFPALGVPLLPIQLLWVNLLTDGLPALALGVDPVDDRLMERPPRPRGERLVSW
                     RRLRLLLARAAPIAASAVASLAVARFVWDEPWPHARATMFTVLVVAHLLYAFAARRRG
                     VALLGNRALLLAVGGGIALQLLVVALPAAHGLFGTATLTLREWALVAAGGVLPLPILW
                     MMRR"
     gene            2407..>3685
                     /locus_tag="VEO00_13195"
     CDS             2407..>3685
                     /locus_tag="VEO00_13195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU58984.1"
                     /translation="MSEGPEAGPPAGRESDVALRDGSTVHVRPVRPEDEAAIRDFFEG
                     LSGRSRWLRFFSGAVNVRQVARWASHVDGDTRFRLVATHGAEDRIVGNAGWARVAPER
                     AEVDFAIADAVQGQGLGTILLGQLAEQAHARGIAVFEAHVLPENHAMIEVFRESGFPV
                     EMRSLPGEVLVEVPTSLSAEAIERFERREQTAAAAALRTFLSPSSIAVIGASRTRGTI
                     GGEVFHNLLAAGFEGPVYPVNPKAEAVQSVKAYPSVLDLPGPPDLAVIVVPAALVVDE
                     ARRCGQAGVRALVVISAGFAETGPEGEARQRELVHVCREAGMRLIGPNCMGIMNTAPD
                     VSMNATFAPTMPVHGRVGFMSQSGALGLAVVDRTRMLGLGLSTFISVGNKADVSGNDL
                     LHYWEQDDRTGLVLLYLESFGNPRKFARIARRVA"
BASE COUNT          389 a         1337 c         1445 g          514 t
ORIGIN      
        1 ggccctgttg ctggtggcgg ccgccgcggt ctcgggattc gtcctcggcg agcgggcgga
       61 cgcggtcgcc atcgtggcca tcgtgctgct gaacgccgtc ctcggcgtgg tcgaggaggg
      121 ccgttccgag cgggcgctcg agtcgctgcg cgcgctggag gcgcccgcgg cgcgcgtggt
      181 gcgcggcggg tcggtcgtct cggtcccgtc ccgggaggtg gtgcccggcg acctggtcct
      241 gctggcggcg ggcgatgggg tccccgccga cctcgggctg ctggaagccg acgcgctcga
      301 ggccgacgag tccctgctca ccggggagtc cctcccggtg gcgaagcggg ccgccgtttc
      361 ccgtgcgggt gcgccggcca gcatggccga ggtcccggag gaggcgcggg ccttctccgg
      421 gacgctcgtc acccgggggt caggagccgg gcgcgtcctg gcgaccggga tggccaccgc
      481 cgtcggcgcc atcgcccagc acgtgcgcgc gccgtcgccc ccgaccccgc tgcaggtcga
      541 gatgcggcgg ctgacggcca cgctcgggac gatctgcatc gtggtcgcgg ccgcagtcct
      601 gggccttacc ctgctccgcg ccggatcggg agggatcccg ctcgaccagg ccttcctcgc
      661 ggcggtggcg ctggccgtcg ccgccgtccc cgagggcctg gccaccgtgg tcacggtcgc
      721 cctcgccctc ggcgtgcggc gcatggcgag ccggggggcc atcgtccggc ggctgcccgc
      781 ggtggagacg ctcggcgcga ccgacctgat cctgtcggac aagaccggca ccctgacgga
      841 gaaccggatg cgggtcgagg cggtggccct tcccggccgc gcgccggtcc cccccgaagc
      901 gctcgacacg ccggccgccg agcggatcgc cgaggtcgcc gtgctctgca acgacgcgac
      961 cctcgacccg ccctccggcg acccgctgga ggtggcgctg ctggagatgg ccggtcagga
     1021 gcgggtcgcg gcgctgcgcc agtccaaccc acgcctggcg gtcgagccgt tcgacgccga
     1081 ccggcgccgg atgagcgcgc tgcaccggac caccgccggc tccttcctct tcgtcaaggg
     1141 atccccggag ggcgtgctgg agcgatgttc gggggagacc ctggccgacg ggagtcgcgt
     1201 cgagctcggg ccggaggcgc gggaggcggt cctcgacgcc gccgccgacc tggcggcctc
     1261 cgggatgcgc gtcctcgcgt tcgcggggcg gagcctggcg tcggccccgc ccgacgtcgg
     1321 cgccgcggtg caggacctgg acctgatcgg cctggtcgcg ctgcgcgacc cgctgcggcc
     1381 ggaggcctcg cggaccgtgt cgctcgcggc gtcggccggc gtgcgcatcg tgatggttac
     1441 cggcgaccac ccgcacacgg cgcgggccat cgccgaggcg gccggcgtgg caggccgggg
     1501 cgcggcgatc ctgaccggcg acgacctgcg gggggccggg atcccggagg acctcctggc
     1561 cgcgccggtc tacgcgcggg tcagccccga ccagaagctc gccctggtcg aggcggcgcg
     1621 cgccggcggg cacgtcgtgg cggtgaccgg cgacggggtc aacgacgcgc cggcgctgcg
     1681 ccgggcggac atcggggtgg cgatgggacg gtcgggcagc gacgtggcgc gccaggcggc
     1741 cgacatggtc gtcaccgacg acaacctggc cacgatcgtg gacgcggtgc gggaggggcg
     1801 cgggatctac gacaacgtgc gcagcgtggt cgactacctg gtcgccggca acctctccga
     1861 gatcgcggtg gtggtgacgt gcctgctgct gttcccggcc ctcggcgtgc cgctgctgcc
     1921 gatccagctg ttgtgggtga acctgctgac cgacggcctt ccggccctgg cgctgggcgt
     1981 cgaccccgtg gacgaccggc tgatggagcg cccgccacgg ccccgcggtg agcggctcgt
     2041 ctcctggcga cggctgcgcc tgctcctggc gcgggcggcg cccatcgcgg cgtccgcggt
     2101 cgcgagcctg gccgtcgccc gcttcgtgtg ggacgagccg tggccgcacg cccgggccac
     2161 catgttcacc gtgctcgtgg tggcgcatct cctctacgcg ttcgccgcgc ggcgccgcgg
     2221 cgtggcgctc ctcggcaacc gggcactgct cttggcggtg gggggcggga tcgccctcca
     2281 gctcctcgtg gtggcgctcc cggccgccca cggcctgttc ggtaccgcga ccctgaccct
     2341 gcgcgagtgg gcgctcgtgg ccgccggcgg cgtgctgccg ctgccgatcc tgtggatgat
     2401 gcgcaggtga gcgagggacc cgaggcgggc cctcccgccg ggcgcgagtc cgacgtcgcg
     2461 ctgcgcgacg gctcgaccgt gcacgtccgg cccgtccggc ccgaggacga ggcggcgatc
     2521 cgggacttct tcgaggggct gtcgggccgc tcccggtggc tccggttctt ctccggcgcg
     2581 gtgaacgtcc gccaggtggc gcgctgggcc tctcacgtgg acggcgacac gaggttccgg
     2641 ctggtggcca cccacggcgc cgaggaccgc atcgtcggga acgccggctg ggcgcgcgtc
     2701 gcgccggagc gcgccgaggt cgacttcgcc atcgccgacg cggtccaggg gcagggcctg
     2761 ggcaccatcc tgctcggcca gctggcggaa caggcccacg cccgtgggat cgccgtgttc
     2821 gaggcccacg tcctgccgga gaaccacgcg atgatcgagg tgttccggga gagcggcttc
     2881 ccggtggaga tgcgctcgct gcccggcgag gtgctggtcg aggtcccgac gtcgctgtcg
     2941 gcagaggcga tcgagcggtt cgagcggcga gagcagacgg ccgccgcggc ggcgctgcgc
     3001 acgttcctgt ccccctcgtc gatcgcggtg atcggggcct cgcgaacgcg cggcacgatc
     3061 ggcggtgagg tgttccacaa cctgctcgcc gccggcttcg aggggcctgt gtacccggtg
     3121 aacccgaagg cggaggccgt ccagtcggtg aaggcctacc cgtccgtgct cgacctcccc
     3181 gggccgccgg acctggcggt gatcgtcgtg cccgccgcgc tggtggtcga cgaggcccgg
     3241 cgttgcgggc aggccggcgt ccgggcgctg gtggtgatct cggccggctt cgccgagacg
     3301 ggcccggagg gcgaggcccg ccagcgcgag ctcgtgcacg tgtgccggga ggccgggatg
     3361 cggctgatcg ggccgaactg catggggatc atgaacaccg cgcccgacgt gtcgatgaac
     3421 gccacgttcg cgccgaccat gccggtccac gggcgcgtcg ggttcatgtc ccagagcgga
     3481 gcgctcggct tggccgtggt cgaccggacg cggatgctcg ggctcggcct gtcgaccttc
     3541 atctcggtcg gcaacaaggc cgacgtctcc ggcaacgacc tgctgcacta ctgggagcag
     3601 gacgaccgga ccggcctggt gctgctgtac ctggagtcgt tcggcaaccc ccgcaagttc
     3661 gcccggatcg cccgccgggt cgcgc
//
LOCUS       DASPWO010000579         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1181998, whole genome shotgun sequence.
ACCESSION   DASPWO010000579 DASPWO010000000
VERSION     DASPWO010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181998"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VEO00_13200"
     CDS             <1..417
                     /locus_tag="VEO00_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYU58985.1"
                     /translation="LERTNVRDLTPEMLPWPIDVVTADLSFIALAAVVPALVRCAASS
                     ADLVLLVKPQFESGRESVGRGGVVRDPAARAAAVRRVARALTAAGAGPLAVTPSPLRG
                     PAGNVEFLLHARAGAPGTLTDEEIDAGVGGVGARST"
     gene            414..1364
                     /locus_tag="VEO00_13205"
     CDS             414..1364
                     /locus_tag="VEO00_13205"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HYU58986.1"
                     /translation="MTLVGFVVHTGRPQAVDSAASLVEWLRARGVTTRSLSDEAVGAD
                     EKADTEAFAEGVNLVVSVGGDGTLLRAADLARRADAALLGVNVGRLGFLTAVEPAAAP
                     ALLALALEGRAPVEERMAIEAIPEGCPWERRLWALNEVMAEKRARHRLVTMAVFVDGE
                     YLTTLSGDAVIVATPTGSTAYSFSARGPIVTPRLDAILLTPVSPHMVFDRSLTLDPDE
                     EVALEVRGEEPGLVSADGRPSVEFPIGARVRIRRSDRRARLVRPVDGPSFYRLLREKF
                     DLPVDHLRGGSGGVARPGEHPDRPPPASEPPKGRAPGREG"
     gene            1369..>2363
                     /locus_tag="VEO00_13210"
     CDS             1369..>2363
                     /locus_tag="VEO00_13210"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYU58987.1"
                     /translation="MLVELHIADLGVIEDVTLQFPEGLSVLTGETGAGKTMVTVALGL
                     ALGDRGAAALVRDGARAAVVEARFQAPGGEGFDPVLGEWATDGEIVLARQVGSDGRSS
                     ARIGGRMAPVGTLSRLGPFLVEFHGQSEHQQLLSPAAQTRFLDRFAGTDHLRDLKRFR
                     RAFESLRRGRALLAELDQAAEARARELELLAFQIREIQGVNVREGELEQLSREESRLA
                     NAEDLALLVGEAQELIGAEDAAAERLHRAAAALGRAGRLDGGAEDLARRVWSLAEESA
                     DAGQALRRFRDGIEIDPTRLGVVQERIAAIRELERKYGTGVKAVRAYLRDAEAKLA"
BASE COUNT          282 a          842 c          927 g          312 t
ORIGIN      
        1 ctcgagcgga cgaacgtgcg ggatctgacg cccgagatgc tgccgtggcc gatcgacgtg
       61 gtgaccgccg acctctcgtt catcgcactc gccgcggtcg tcccggcact ggtgcggtgc
      121 gccgcgtcga gcgccgacct cgtgctcctg gtgaagccgc agttcgagtc ggggcgggag
      181 tccgttggcc gcggcggcgt ggtgcgcgac ccggccgcac gggccgcggc cgtccgccgc
      241 gtcgcgcggg ccctgaccgc cgcgggggct ggaccactcg ccgtgacgcc ctcgccgctg
      301 cgcggccccg ccggcaacgt cgagttcctg ctccacgcgc gcgccggcgc gcccggcacc
      361 ctcaccgacg aagagatcga cgctggggtg gggggggtcg gggcgaggag cacatgaccc
      421 tggtcgggtt cgtggtgcac accgggcggc cgcaggcggt ggactccgcc gcctcgctcg
      481 tcgagtggct gcgcgcccgc ggggtcacca cccgatcgct gtccgacgag gccgtcgggg
      541 ccgacgagaa ggcggacacg gaggccttcg ccgagggcgt gaacctcgtg gtgtcggtcg
      601 gcggggacgg cacgctcctg cgggcagcgg acctggcgcg ccgggccgac gcggcgctcc
      661 tcggggtgaa cgtcggccgc ctgggattcc tcaccgcggt cgagcccgcc gccgccccgg
      721 cgctcctggc gctcgccctg gaaggccgcg cgccggtgga ggagcgcatg gcgatcgagg
      781 cgattccgga ggggtgcccc tgggagcgcc ggctctgggc cctgaacgag gtgatggccg
      841 agaagcgagc gcggcaccgc ctggtgacga tggcggtgtt cgtcgacggt gagtacctca
      901 ccacgctttc gggcgacgcg gtgatcgtgg ccacgccgac cggctccacc gcctactcgt
      961 tctcggcgcg cgggccgatc gtcacgccgc ggctcgacgc gatcctgctc accccggtca
     1021 gcccgcacat ggtgttcgac cggtcgctga cgctcgaccc cgacgaggag gtggccctgg
     1081 aggtgcgcgg cgaggagccc ggcctggtat cggccgacgg ccgcccctcc gtcgagttcc
     1141 cgatcggcgc ccgagtgcgc atcaggcgct ccgaccggcg agcgcgcctg gtccggccgg
     1201 ttgacggccc gtcgttctac cggctgctgc gcgagaagtt cgacctaccc gtggaccacc
     1261 ttcgcggggg gtctgggggg gtagctcgcc ctggggagca ccccgaccga ccacctccgg
     1321 cgagcgaacc ccccaaggga agagctcccg ggcgggaggg ctgagccgat gctcgtcgag
     1381 ctgcacatcg ccgacctcgg cgtgatcgag gacgtcacgc tgcagttccc ggagggcctg
     1441 tcggtgctca ccggcgagac cggcgccggc aagaccatgg tcacggtggc tctcggcctg
     1501 gcgctcggag accgcggcgc ggcggcgctg gtccgcgacg gcgcccgggc cgcggtcgtc
     1561 gaggcgcgct tccaggcccc cggcggcgag ggcttcgacc cggtcctcgg agagtgggcc
     1621 accgacggtg agatcgtcct cgcgcgtcag gtcgggtccg acggccggag ttccgcgcgg
     1681 atcggcgggc ggatggcgcc ggtcgggacg ctctccaggc tcgggccgtt cctggtcgag
     1741 ttccacgggc agagcgagca ccagcagctg ctgtcgcctg ccgcacagac gcgcttcctg
     1801 gatcgcttcg ccggaaccga ccacctccgc gatctgaagc ggttccggcg ggcgttcgag
     1861 agcctgcgcc gcgggcgggc gctcctcgca gagctcgatc aggcggccga ggcccgtgcc
     1921 cgcgagctgg agctgctcgc cttccagatc cgggagatcc agggcgtgaa cgtgcgcgag
     1981 ggcgagctgg agcagctctc ccgcgaggag tcgcgcctgg cgaacgccga agacctcgcg
     2041 ctgctcgtcg gcgaggcgca ggagctgatc ggggccgagg acgccgcggc ggagcgcctg
     2101 caccgcgcgg ccgcggctct ggggcgggcc ggccgcctgg acggcggtgc cgaggacctc
     2161 gcccgccggg tctggtcgct ggccgaggag tcggcggacg ccggccaggc gctgcgccgg
     2221 ttccgtgacg gtatcgagat cgacccgacc cggctcggtg tggtgcagga gcgaattgcg
     2281 gcgatccggg agctggagcg caagtacggg accggcgtga aggccgtccg cgcgtacctg
     2341 cgggacgcgg aggcgaagct cgc
//
LOCUS       DASPWO010000580         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_673883, whole genome shotgun sequence.
ACCESSION   DASPWO010000580 DASPWO010000000
VERSION     DASPWO010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673883"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VEO00_13215"
     CDS             <1..729
                     /locus_tag="VEO00_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HYU58988.1"
                     /translation="PAPVAETVERVTAALGYDPDAFPDAAEEVTRQADLARLRAMAEE
                     FGAAHPSGDAAAFVAELGRRFSAEREGRGVNLLTYHRAKGLEWDAVFLPRLLDGELPF
                     RSGRSRAPEDEERRLLYVGITRARRHLCLTWSLDARAGRSPFLTELTDEPPGPSRPSA
                     RATVKTAAGDGSPLFEALRTWRRDRAKADGVPAYVVFHDATLAAIAEQRPASRVQLRR
                     VPGVGPAKIERYGDEVLAVVAGAR"
     gene            complement(796..1311)
                     /locus_tag="VEO00_13220"
     CDS             complement(796..1311)
                     /locus_tag="VEO00_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HYU58989.1"
                     /translation="MFDLGAPLWQIALRALAVYAAVFLGFRVLGKRELGQMTVFDLVL
                     ILLLSNAVQNAMVGPDTSLQGGLVAAAVLLVANRLVAETRLRSPLLDRLFEGRSAVLV
                     EHGKLVTGQLRRQGVAEEDLAMAMREHGIASLDDVQLAVLETDGSISIVPASSPTLRS
                     HRRRARALRRK"
     gene            1514..1873
                     /locus_tag="VEO00_13225"
     CDS             1514..1873
                     /locus_tag="VEO00_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58990.1"
                     /translation="MEAGLLYRYGKPVAGHEREAIALFAEVLEFWMEKRERGEITFFE
                     PFIVETSDFDEDAGFWVIKGPEEKIAAFRHEDRYQKILMKASIFLAHLTVATLRVGDA
                     VNEQVDLLGKVYAEVGV"
     gene            complement(1951..2736)
                     /locus_tag="VEO00_13230"
     CDS             complement(1951..2736)
                     /locus_tag="VEO00_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYU58991.1"
                     /translation="MLTDEVRAAWWWAQDRQERVERHPWGVALADTRWPRVWDANFVR
                     VERAPVTLAEAERAMAAVLERTGGTHARLWVPEPERVPGFVEAAAEAGYGENRDLAML
                     LDGEPASAPGRPAAVEVRWIDHTDPDLARAMRQYRDARQIDDEVMDQLLARDAWLATL
                     GGVRWFAGYLGGEIAGFCAVATAPNGVSMIDDVTTGEAFRRRGVATAVVTAATNAAQR
                     ASSVQRTFLTTGTDNAPAIAVYERLGFHAIAEVIDLHRGAARW"
     gene            2788..>3246
                     /locus_tag="VEO00_13235"
     CDS             2788..>3246
                     /locus_tag="VEO00_13235"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HYU58992.1"
                     /translation="MITCRVYHDGKLEKEGFDPQEVSDHLEHGDLVWLDVLEPTDEEI
                     ALCQKEFSLHPEAVEDVRERNQRTKVDLYEGYFVMVAYGVAGGPSGIETHEVHVFAGR
                     NFLLTFRFDPPFDLGQVLHRWDASPEITSHGGGALLHFLLDEIIDSYFPVV"
BASE COUNT          455 a         1171 c         1163 g          457 t
ORIGIN      
        1 cccgccccgg tggccgagac ggtcgagcgc gtcaccgccg ccctcggcta cgaccccgac
       61 gcgttccccg atgccgccga ggaggtgacc cggcaggccg acctggcccg gctgcgcgcg
      121 atggccgagg agttcggagc ggcacacccc tccggggacg cggcggcgtt cgtcgccgag
      181 ctggggcggc ggttctcggc cgagcgagag ggccgcgggg tgaacctgct cacctaccac
      241 cgcgccaagg gcctggagtg ggatgcggtg ttcctgccgc gcctcctgga tggtgagctg
      301 cccttccgct ccgggcgcag ccgcgcgccg gaggacgagg agcgccgcct gctctacgtg
      361 ggcatcaccc gggcccgccg gcatctctgc ctgacctggt cgctcgacgc ccgcgcgggc
      421 cgcagcccat tcctgaccga gctgaccgac gagccgcccg ggccgtcccg cccatcggcg
      481 agggccacgg tgaagaccgc agcgggggac gggtcgccgc tgttcgaggc gctccgaaca
      541 tggcgccgcg accgggcgaa ggcggacggc gtgccggcct acgtggtctt ccacgacgcg
      601 acgctcgcgg cgatcgccga gcagcgcccc gcgtcccgcg tccagctgcg ccgcgttccg
      661 ggcgtgggcc cggccaagat cgagcgctac ggcgacgagg tgctggccgt ggtcgccggg
      721 gcccgctgag gacgaacagg cggtcgagga gcccgccggc actcctcgcg ccggcttcgt
      781 ccgacccgtt cgggctcact tcctgcggag ggcgcgggcg cggcgccggt gggatcggag
      841 ggtgggggag ctcgccggca cgatgctgat cgagccgtcg gtctcgagca ccgccagctg
      901 gacgtcgtcg agcgatgcga tgccgtgctc acgcatcgcc atcgccaggt cttcctcggc
      961 caccccttgc cggcggagct gcccggtgac gagcttcccg tgctccacca ggacggccga
     1021 gcggccctcg aacaggcggt ccaggagcgg gctgcgcagg cgggtttccg cgaccagccg
     1081 gttggcgacg agcagcaccg ccgccgccac aaggcccccc tgcagggacg tgtccggtcc
     1141 gaccatggcg ttctggaccg cgttcgacag cagcaggatc agcaccaggt cgaacacggt
     1201 catctgcccg agctcccgct ttcccagcac ccggaaaccc aggaacacgg cggcgtagac
     1261 ggccagcgcc cgaagggcga tctgccagag gggcgcgccc agatcgaaca tgtcgttcct
     1321 ccgatccgtc cggggacgat gtcgatccat tctgaacgcc ggtgcggtcc cgcccgggga
     1381 gggaccttcg accctggtcc ccgcgcgctc agtgcagtcg aatgcggtca tcgactccgg
     1441 gcgccggagg aggtgatcgg ggcatccgcg ggtcgacccg gaaggcttcc cgtaagccga
     1501 gaggggtgga cgtatggaag ccgggctgtt gtaccgctat ggcaagccgg tcgccggcca
     1561 cgagcgcgag gcgatcgcct tgttcgccga ggtcctcgag ttctggatgg agaagcgcga
     1621 gcggggcgag atcaccttct tcgagccctt catcgtggag accagcgact tcgatgagga
     1681 cgcggggttc tgggtgatca agggccccga ggagaagatc gcggcgttcc gtcacgagga
     1741 tcgctaccag aagatcctca tgaaggcgtc gatcttcctc gcgcatctca cggtcgccac
     1801 gctcagggtg ggcgacgcgg tgaacgagca ggtcgacctg ctcgggaagg tgtacgccga
     1861 ggtcggcgtc tgacgacggc gatcttcagg cccgctcacg aggcggcggg ggctcgcgtc
     1921 ccgccgcctc gaccttcctt cgcacccctc tcaccaccgt gcggcgccgc ggtggaggtc
     1981 gatgacctcg gcgatcgcgt ggaagcccag gcgctcgtag acggcgatcg cgggggcgtt
     2041 gtcggtcccg gtggtgagga acgtccgttg gacgctcgac gccctctggg cggcgttcgt
     2101 cgcggcggtc accaccgccg tggccacccc cctgcggcgg aacgcctcgc cggtggtgac
     2161 gtcgtcgatc atcgagacgc cgttcggggc ggtggccacg gcgcagaagc cggcgatctc
     2221 cccgcccagg tagccggcga accaccgtac gccgccgagc gtcgcgagcc acgcgtcgcg
     2281 cgccagcagc tggtccatga cctcgtcgtc gatctggcgg gcgtcccggt actggcgcat
     2341 cgcgcgggcc aggtccggat cggtgtgatc gatccagcgc acctcgacgg cggccgggcg
     2401 gcccggcgcc gacgccggct cgccgtcgag cagcatcgcc agatcacggt tctctccgta
     2461 cccggcctcc gcggcggcct ccacgaaccc cgggacgcgc tcgggttcgg ggacccacag
     2521 ccgggcgtgg gtgccgccgg tgcgctccag gaccgcggcc atggcccgct ctgcctcggc
     2581 cagggtcacc ggggctcgtt ccacccggac gaagttcgcg tcccacaccc gcggccagcg
     2641 cgtgtcggcg agggcgactc cccagggatg gcgctcgacc cgctcctggc gatcctgggc
     2701 ccaccaccac gcggcgcgca cctcgtcggt gagcacaacg cggagcctac cgccgggaac
     2761 gccacgaccc gcgctacagt ccttgtcgtg atcacctgcc gcgtctacca cgacgggaag
     2821 ctcgagaagg aggggttcga cccccaggag gtctccgacc acctcgagca cggcgacctg
     2881 gtctggctcg acgtcctcga accgaccgac gaggagatcg cgctgtgcca gaaggagttc
     2941 tctctccacc ccgaggccgt tgaggacgtc cgcgagcgca accagcggac gaaggtcgac
     3001 ctctacgagg ggtacttcgt gatggtggcg tacggggtcg ccggggggcc gagcgggatc
     3061 gagacgcacg aggtgcacgt gttcgcgggg cgcaacttcc tgctcacctt ccgcttcgac
     3121 ccgccgttcg acctcgggca ggtcctccac cgctgggacg cctcgccgga gatcacgagc
     3181 cacggcggcg gcgcgctgct gcacttcctg ctcgacgaga tcatcgactc gtacttcccg
     3241 gtggtc
//
LOCUS       DASPWO010000581         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_760644, whole genome shotgun sequence.
ACCESSION   DASPWO010000581 DASPWO010000000
VERSION     DASPWO010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760644"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VEO00_13240"
     CDS             <1..458
                     /locus_tag="VEO00_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HYU58993.1"
                     /translation="GREYALRHALEDLAMRESFDVVLIDCPPSLGVLTVNGLTAADDV
                     LIPLQCEALSHRGVGQLLDTIADVRRMTNRDLRIGGVVPTMFDGRTRHAREVLEDVGE
                     RYGLEVLSPPVRKSIRFAEAPREGKTILRFAPRHPGSEAYREIARGLVS"
     gene            455..790
                     /locus_tag="VEO00_13245"
     CDS             455..790
                     /locus_tag="VEO00_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU58994.1"
                     /translation="MTGGGGGAPDRGDAAAHRRHPSAGAARLVPRAPDPLGKMALYSG
                     VGPERPFGTFLVDCSACRRETAVRPADLLRAALPVSLHLPVVRRYPSYMRCPSCGRRA
                     WLRVSWTLS"
     gene            872..1447
                     /locus_tag="VEO00_13250"
     CDS             872..1447
                     /locus_tag="VEO00_13250"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HYU58995.1"
                     /translation="MDDDRERDDVGGHDEPFEGGEPFPEKDDDGPTPLDGEERENVEA
                     DLEDLDAMRVVFEGQGFRGVVIACPDCHQNHYYAWELIRESLEHMLETGEARMHEPAF
                     DPHEDEYVLWDYGKGYIDALADAGLDEGSRVEVTACAWCDTPVEPHFGFCPKCGRPLA
                     AARLLRELVARGMEEREARALLVKAGFEPFS"
     gene            complement(1501..>2939)
                     /gene="ligD"
                     /locus_tag="VEO00_13255"
     CDS             complement(1501..>2939)
                     /gene="ligD"
                     /locus_tag="VEO00_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HYU58996.1"
                     /translation="AGGAPTPEGNSFVIQKHNATRLHYDFRLERDGVLVSWAVPKGLP
                     LEPGEKRLAVHTEDHPIEYGSFEGWIPKGHYGAGEVKLFDRGTYETLEWSEGKVGVRL
                     HGERHRGEYHLVKTRSNDWIVFLSKRSVEEQPPAPPAYAPMLAEAGGKAFDDPGWRFE
                     PKLDGIRTLAYVGTDETRLLSRTGRDQTGIYPELGRLSEQVNALSAVIDGEIVALDEE
                     GRPSFERLQQRMNLHAAREVEQMRRSVPVILYAFDLLWMDGEPWIGRPLEERRDRLEA
                     IVTEGRRIQLTSYVDERGTAFHETAKRLGLEGVIAKRLGSAYQPGRRSRDWRKIKILN
                     RQDVVLLGWTPGTGGRANAFGALLVGANVGGRGRAKPALRWIGQVGTGFTERMLADLL
                     ARLREIEAKDPPIDDPALRRVKGARWVRPELVAEVEYLQQTAGGKLRAPSFKGLRPDK
                     TPEDAFLEPPAPAAGSRKRATRPAAASP"
BASE COUNT          365 a         1092 c         1049 g          433 t
ORIGIN      
        1 ccggccgcga gtacgcgctc cgccacgccc tggaggacct ggcgatgcgc gagtcgttcg
       61 acgtggtcct gatcgactgc cctccctcgc tcggggtgct gaccgtgaac ggcctgacgg
      121 ccgcggacga cgtgctgatc cccctgcagt gcgaggcgct gtcgcaccgc ggggtcggac
      181 agctactcga cacgatcgcc gacgtgcggc ggatgacgaa ccgcgacctg cgcatcggcg
      241 gggtggtgcc gacgatgttc gacgggcgca cccggcatgc gcgggaggtg ctcgaggacg
      301 tcggggagcg ctacggcctg gaggtcctct ctccgcccgt gcgcaagagc atccgcttcg
      361 ccgaggcccc gcgcgagggg aagaccatcc ttcgcttcgc tccacgccac ccgggttcgg
      421 aggcctaccg tgagatcgcc cgggggctcg tgtcgtgacc ggcggcggcg gcggcgcccc
      481 ggaccgcggc gacgccgcgg cccaccgccg ccatccgagc gccggggccg cgcgcctcgt
      541 cccgcgtgcg ccggacccgc tcgggaagat ggcgctctac tcgggggtcg ggcccgagcg
      601 ccccttcggc accttcctgg tggactgctc ggcctgccgc cgggagaccg cggtgcgccc
      661 ggccgacctg ctgcgcgcgg cgctcccggt gtcgctgcac ctgccggtgg tccgccgcta
      721 cccctcctac atgcgctgtc cgtcgtgcgg ccggcgcgcc tggctgcgcg tcagctggac
      781 cctgtcctga ggggtggctt tcggggggaa cacggggggc ggagcccccc gtggaggggt
      841 cgcttctcgc gcgacctgcc agactgagtg ggtggacgac gatcgtgagc gcgacgacgt
      901 cggcgggcac gacgagccgt tcgagggcgg cgagccgttc cccgagaagg acgacgacgg
      961 gccgacccca ctcgacgggg aggagcgcga gaacgtcgag gcggacctgg aggacctcga
     1021 cgcgatgcgc gtggtcttcg agggccaggg tttccgcggc gtggtgatcg cctgcccgga
     1081 ctgtcaccag aaccactact acgcctggga gctcatccgg gagagcctcg agcacatgct
     1141 cgagaccggg gaggcccgga tgcacgagcc ggccttcgac ccgcacgagg acgagtacgt
     1201 cctgtgggac tacggcaaag gctacatcga cgcgctggcc gatgccgggc tcgacgaggg
     1261 gtcccgggtc gaggtcaccg cctgcgcgtg gtgcgacacg ccggtcgagc cccacttcgg
     1321 cttctgtccg aagtgtggcc gcccgctcgc ggcggcgcgc ctgctccgcg agctggtcgc
     1381 ccgcggcatg gaggagcgcg aggctcgcgc cctgctggtc aaggccggct tcgagccgtt
     1441 ctcctgaccc cgccgcacgg cctttcacct cgccgcctcg cggaaatctg atcccgacgt
     1501 tcatggactg gccgctgcgg gccgggtggc ccgcttcctg gagccggccg cgggcgcggg
     1561 tggctccagg aaggcgtcct cgggggtctt gtccgggcgc aggcccttga aggacggcgc
     1621 gcgcagcttc ccgccggccg tctgctgcag gtactccacc tcggcgacca gctcgggccg
     1681 cacccagcgc gcgcccttca cccggcgcag cgcggggtcg tcgatcggcg gatccttcgc
     1741 ctcgatctcg cgcaggcgcg ccagcaggtc ggcgagcatc cgctcggtga agccggtccc
     1801 gacctggccg atccagcgca gcgcgggctt tgcccgtccc ctccctccga cgttcgcgcc
     1861 gacgaggagc gccccgaacg cgttcgcccg gccgccggtc cccggcgtcc accccagcag
     1921 caccacgtcc tgccggttca ggatcttgat cttccgccag tcccgtgacc gccggcccgg
     1981 ctggtaggcg ctccccagcc gcttcgcgat cacgccctcc agaccgagcc gcttcgcggt
     2041 ctcgtggaag gccgtcccgc gctcgtcgac gtagctcgtc agctggatgc gccggccctc
     2101 ggtgacgatc gcctcgaggc ggtcgcggcg ctcctcgagg gggcggccga tccacggctc
     2161 gccgtccatc cacagcaggt cgaacgcgta gaggatgacg ggcaccgatc gccgcatctg
     2221 ctcgacctcg cgcgcggcgt gcaggttcat ccgctgctgc agccgctcga acgacggccg
     2281 accctcctcg tccagcgcga cgatctcgcc gtcgatcacg gccgagagcg cgttgacctg
     2341 ctccgagagc cgcccgagct ccgggtagat gccggtctgg tcgcggccgg tgcgcgagag
     2401 cagccgcgtc tcgtcggtcc cgacgtacgc gagcgtgcgg atgccgtcga gcttcggctc
     2461 gaaccgccac cccggatcgt cgaaggcctt tccgccggcc tcggcgagca tcggcgcgta
     2521 cgcgggcggc gccggcggct gctcctcgac cgagcgcttg gacaggaaca cgatccagtc
     2581 gttgctgcgg gtcttgacaa ggtggtactc gccgcgatgg cgctcaccgt gcagccggac
     2641 gccgaccttg ccctcggacc actccagcgt ttcgtaggtc ccccggtcga agagcttcac
     2701 ctcgcccgcg ccgtaatgcc ccttcgggat ccagccctcg aaggagccgt actcgatcgg
     2761 gtggtcctcg gtgtgcaccg cgagccgctt ctcgccgggc tcgagcggca gccccttcgg
     2821 cacggcccag gaaacgagca cgccgtcgcg ctccagccgg aagtcgtagt gcagccgcgt
     2881 ggcgttgtgc ttctggatga cgaacgagtt gccctcgggg gtgggcgccc ccccggcgg
//
LOCUS       DASPWO010000582         7457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_930530, whole genome shotgun sequence.
ACCESSION   DASPWO010000582 DASPWO010000000
VERSION     DASPWO010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930530"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VEO00_13260"
     CDS             complement(<1..187)
                     /locus_tag="VEO00_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018531886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HYU58997.1"
                     /translation="MAKTRTLLACTACGRPTDRWTGRCAACGAWGSIEEHPAAALSGA
                     APGRVPGGAPAVVLPLEG"
     gene            227..547
                     /locus_tag="VEO00_13265"
     CDS             227..547
                     /locus_tag="VEO00_13265"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYU58998.1"
                     /translation="MTVVTVTGLAGGRGLRIVGELDASNVFDLTERLEETVTGEGDVT
                     LDLRDLRFIDSAGIHALIRAAKQLEGRGRMRLLVVPGPVLRVIELVGIRESLPHVDVE
                     TEER"
     gene            576..980
                     /locus_tag="VEO00_13270"
     CDS             576..980
                     /locus_tag="VEO00_13270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYU58999.1"
                     /translation="MALLTLSVMVAVRCRLVEVQAARRRVRALAERAGLGRAAVDDVE
                     LAVSEACTNAIQHGCPASAEHVEIAFRSDDRCVEIEVRDSGIFRKRIPMPELHEARGR
                     GIPLMIALMDEVHITEGTRRKPGTVIRLVKCL"
     gene            982..3126
                     /locus_tag="VEO00_13275"
     CDS             982..3126
                     /locus_tag="VEO00_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYU59000.1"
                     /translation="MSQPPAESFPRRYARTHRFRLGEPRSLTVTDDGARVLFLRSPAG
                     DDPVNSLWSFDTGSRREQLIVDARDLGAGVDAELSPDERARRERARETAGGIVDYAVD
                     RPGRIAAFALDGGLFVADLVNGGGRALPAAIPAFDPRPAPGGERVSYVCQGALRMVDL
                     ETGEDREVSSDPDDAVSWGVAEFVASEEMDRYRGHWWSPEGQALLAARVDESSVRRWH
                     IASPEDPEREPVVVRYPVAGTANAIVTLHLLDLDGGRVDVAWDRAAFEYVVAVTWPRD
                     GQPTVLVQSRDQRRWQILQVDPATGRTDIAHRDASDRWLEILRGVPAFTDDGRLVFVT
                     ESDDTRRLTLDGLPVTPPGLQVRRVEHVGGDVLFTGSETDPEEVHLYRLDAGGTVTRL
                     TEEPGVHGGTGGGDVTVRTAESMDWPGARTAVWRGDEPIGEIASNAEPPVIEPDVTLL
                     RAGPRGLSAALLRPSGWDGRGRLPVLLSPYGGPQFQQVMRSRRHYLLAQWLADQGFAV
                     LVIDGRGTPGRGLAWEHAVWRDFGVTLEDQVDGLHAVAAEHPELDLGRVGIRGWSFGG
                     TLAAMAVLRRPDVFHAAVAGAAGTDERLYDTHYTERYLGNPNEEPEVYDRNSVVSYAA
                     GLERPLLLVHGMADDNVVVANTLRLSRALLQAGRPHQVLPLSGITHMTNEPATAENLL
                     RLEVEFLRQALGVPPPGPVRREVRPPAGPGRR"
     gene            complement(3083..3529)
                     /locus_tag="VEO00_13280"
     CDS             complement(3083..3529)
                     /locus_tag="VEO00_13280"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HYU59001.1"
                     /translation="MSGPDRALVEARLASISETLRALESLAGMSLDDYRADVVRRSAV
                     ERLLARLVEVAAEINAHLAARTLGAAPADYRDSFLKMAEAGVLDARFAADVARSVGLR
                     SRLVHEYEAVDHAVVHAAIPIAIEQYRRYAEAVTAYLDRLEGEPPA"
     gene            complement(3526..3966)
                     /locus_tag="VEO00_13285"
     CDS             complement(3526..3966)
                     /locus_tag="VEO00_13285"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYU59002.1"
                     /translation="MGMDLERGRLDVVRRLAARRDGLVRLCRERDVRLLILFGSAASG
                     SQRPDSDIDLAVDFGRDTSPRDELAFHGDLMEALDTDRVDLVNVRTAGPIVLREIALH
                     GTPVYVRAERDFAEFQVHALTRFMDTARFRELRGRLVRARFGSR"
     gene            complement(4089..6566)
                     /locus_tag="VEO00_13290"
     CDS             complement(4089..6566)
                     /locus_tag="VEO00_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019436145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HYU59003.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLEAVRQQVEEIIGQGQAAPTGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLQKLGADLNRVRQQVIQLLSGYTGGKGEGQPGEQTPQ
                     GSLVLDQFGRNLTQNAREGKLDPVIGREKEIERVMQVLSRRTKNNPVLIGDPGVGKTA
                     IVEGLASAIVKGEVPETIKGKQIYTLDLGALVAGSRYRGDFEERLKKVLKEIKTRGDI
                     ILFIDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTRDEYRKHLEKDAALER
                     RFQPIYVEEPSVAHTIDILKGLRDRYEAHHRVSFTDEALVAAANLADRYISDRYLPDK
                     AIDLIDEAGSRMRIRRMTAPPEVREIDEKLADVRRQKESSIDAQDFEGAARLRDEERR
                     LGEERTQREQEWKSGELDVLAEVDEEEIAEVLSAWTGIPVYKLTEEETQKLLRMEDEL
                     HKRIVDQVEAVTAVSKAIRRTRAGLKDPKRPSGSFIFLGPSGVGKTELSKALAEFLFG
                     DEDALIQLDMSEYMEKHTVSRLVGSPPCYVGYEEGGQLTEAVRRKPFSVVLFDEIEKA
                     HPDVFNTLLQILEDGHLTDAQGHQVDFKNTVIIMTSYLGTRYLQTIAVGFAAQADEQV
                     TYDKMKAKVTDELKRSFRPEFLNRIDEVIVFHSLSREDVKAIVDLMMRRVEEQLKSKD
                     VDIVLTEPAKNLLAEKGYDPSLGARPLRRTIQRMVEDPLSEKLLWKEFAAGETVIVDV
                     AEEEITFEHAPTPPATPPVEMAGQQES"
     gene            6921..7163
                     /locus_tag="VEO00_13295"
     CDS             6921..7163
                     /locus_tag="VEO00_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59004.1"
                     /translation="MSEHVDQHHEHGGRVHGHKHFHVTHYLRTGEDWAHLTAVHEHEH
                     NHPQIAHTHKPHEDEAHEHTREAHVHDHARPAESPG"
     gene            complement(7174..>7457)
                     /locus_tag="VEO00_13300"
     CDS             complement(7174..>7457)
                     /locus_tag="VEO00_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59005.1"
                     /translation="LPVALGPDDHTFVDELQLGAFAVPGRDEVSEPWLVPDAPWRPVM
                     RTFTSDGERTIVLERDRGPFWPAWHPPAPSPKYRSSRLGITTGPDDPLH"
BASE COUNT         1001 a         2689 c         2670 g         1097 t
ORIGIN      
        1 cgccttcgag cgggaggacc acggccggcg cgcccccggg aacccggccg ggcgccgcgc
       61 cggagagggc cgcggcggga tgctcctcga tggaccccca ggcgccgcac gccgcgcagc
      121 gtcccgtcca ccggtcggtg ggccggccgc acgccgtgca ggccaggagc gtccgggtct
      181 tcgccatgtg ttgcacccta gccggggggt ctgacacgct ttcgccgtga ccgtcgtgac
      241 ggtgaccggc ctggccggag gacgcgggct gcggatcgtg ggggaactcg acgcctcgaa
      301 cgtgttcgac ctgaccgagc gtctggagga gacggtgacg ggcgagggcg acgtgacgct
      361 cgacctgcgc gacctgcggt tcatcgactc ggcggggatc cacgcgctga tccgagcggc
      421 gaagcagctc gaggggcgcg gccggatgcg cctcctcgtc gtccccggcc cggtcctgcg
      481 cgtgatcgag ctcgtcggga tccgggaatc gctgccgcat gtcgacgtcg agaccgagga
      541 gcgctgatgg ccccgacggg ccaccggccc gcacgatggc cctgctgacc ctctcagtga
      601 tggtggcggt ccgctgccgg ctggtggagg tgcaggccgc ccggcggcgg gtgcgcgcgc
      661 tcgccgagcg ggccggcctg ggccgcgcgg ccgtggacga cgtggagctc gcggtcagtg
      721 aggcctgcac gaacgccatc cagcacggct gcccggccag cgccgagcac gtcgagatcg
      781 cgttccgcag cgacgaccgc tgcgtcgaga tcgaggtccg cgactccggg atcttccgca
      841 agcggatccc catgcccgag ctccacgagg cccgcggccg gggcatcccg ctgatgatcg
      901 cgctgatgga cgaggtgcac atcaccgaag ggacgcgccg caagccggga acggtgatcc
      961 gcctggtgaa gtgcctgtga cgtgagccag cccccggcgg agtcgttccc ccgacggtac
     1021 gcccgcaccc acaggttccg gctcggcgag ccccgctcgc tcacggtcac cgacgacggc
     1081 gcgcgcgtgc tgttcctgcg ctcgccggcc ggagacgacc ccgtgaactc gctctggtcg
     1141 ttcgacaccg gaagccgccg agaacagctg atcgtcgacg cgcgcgacct cggggcgggc
     1201 gtcgacgccg agctgtcgcc ggacgagcgc gcgcgacggg agcgggcccg cgagacggcg
     1261 ggcggcatcg tggactacgc cgtcgatcgc cctgggcgca tcgcggcctt tgcgctcgac
     1321 ggcggcctgt tcgtggccga tctcgtgaac ggcggcgggc gagcgcttcc ggccgccatc
     1381 cctgccttcg atcctcggcc ggcgccgggc ggcgaacggg tctcgtacgt ctgccagggg
     1441 gcgctgcgca tggtcgatct cgagaccggc gaggaccgcg aggtgtcgtc ggacccggac
     1501 gacgccgtgt cctggggcgt cgcggagttc gtcgcgtccg aggagatgga tcgttaccgc
     1561 gggcactggt ggtcgccgga ggggcaagcg ctcctcgccg cgcgcgtcga cgagtcgagc
     1621 gtccgtcgct ggcacatcgc gtcgcccgag gaccccgagc gcgagccggt cgttgtccgc
     1681 tacccggtgg cagggaccgc caacgcgatc gtcacgctgc acctgctgga cctggacggc
     1741 ggccgcgtcg atgtggcgtg ggaccgcgcc gcgttcgagt acgtcgtggc cgtgacgtgg
     1801 ccgcgcgacg gacaacccac cgtgctcgtg cagagccgcg accagcgccg gtggcagatc
     1861 ctgcaggtcg acccggccac cggccgaacc gacatcgcgc atcgggacgc ctcggaccga
     1921 tggctcgaga tcctgcgcgg tgtcccggcg ttcaccgacg acggccggct ggtgttcgtc
     1981 acggagtccg acgacacgcg ccgcctgacg ctcgacggtc tgccggtcac cccgccggga
     2041 ctgcaggttc gccgagtgga gcacgtgggc ggggacgtcc tattcacggg ctccgagacg
     2101 gaccccgagg aggtgcacct ctaccgcctc gacgccggcg gaacggtcac gcgcctgacc
     2161 gaggagcccg gtgtccacgg cgggacgggc ggtggagatg tcaccgtccg caccgcggaa
     2221 tccatggact ggcccggggc ccgtaccgcc gtctggcgcg gggacgagcc gatcggcgag
     2281 atcgcctcga acgccgagcc gccggtgatc gagccggacg tgacgctcct ccgcgccggc
     2341 ccgcggggcc tgagcgccgc gctcctgcgc cccagcggat gggacggccg cggccgcctg
     2401 cccgtcctgc tcagcccgta cggcggcccc cagttccagc aggtgatgcg gagccgccgc
     2461 cactacctgc tggcccagtg gctcgccgac cagggcttcg cggtcctggt gatcgacggg
     2521 cgcggcaccc ccgggcgcgg gctggcgtgg gagcacgcgg tctggcgaga cttcggggtc
     2581 acgctcgagg accaggtcga cgggctccac gcggtcgccg ccgagcaccc ggagctcgac
     2641 ctggggcggg tcgggatccg cgggtggtcg ttcggcggca ccctggccgc gatggccgtg
     2701 ctccgccggc ccgacgtgtt tcacgccgcg gtggccgggg ccgccggcac ggacgagcgg
     2761 ctgtacgaca cgcactacac cgagcgctac ctggggaacc cgaacgagga gcccgaggtc
     2821 tacgaccgca actcggtcgt ctcctacgcg gccgggctcg agcgcccgct gctgctggtg
     2881 cacgggatgg ccgacgacaa cgtggtggtg gcgaacaccc tgcgcctgtc gcgcgcactg
     2941 ctccaggccg gccgcccgca ccaggtgctg cccctctccg gcatcaccca catgacgaac
     3001 gagccggcta cggccgagaa cctgctccgg ctggaggtgg agttcctgcg ccaggcgctc
     3061 ggggtgcccc cgccgggccc ggtcaggcgg gaggttcgcc ctccagccgg tccaggtagg
     3121 cggtgaccgc ttccgcgtag cgccggtact gctcgatcgc gatcgggatc gccgcgtgca
     3181 cgacggcgtg gtcgacggcc tcgtactcgt gcaccagacg gctgcgaagc ccgaccgagc
     3241 gcgcgacgtc ggccgcgaag cgcgcgtcga gcacgcccgc ctcggccatc ttcaggaacg
     3301 agtcgcggta gtcggcggga gcggcgccca gggttcgagc ggccagatga gcgttgatct
     3361 ccgcagccac ctccaccagc cgggccagga gtcgctccac ggcgctccgt cgcacgacgt
     3421 ccgcccggta gtcatccagg gacatcccgg ccaggctctc gagggcccgc agcgtctccg
     3481 agatggacgc gaggcgggct tccacgaggg cgcggtcggg gccgctcatc tcgacccgaa
     3541 tcgggcccgg accagccgcc cgcgaagttc gcggaaccgc gcggtgtcca tgaacctggt
     3601 cagcgcgtgg acctggaact cggcgaagtc acgctccgcc cgcacgtaca ccggcgtccc
     3661 gtgcagcgcg atctcccgca ggacgatcgg cccggcggtc cggacgttca cgaggtccac
     3721 gcgatcggta tcgagagcct ccatcaggtc gccgtggaag gcgagctcgt cccggggaga
     3781 ggtgtcgcgg ccgaagtcga cggccaggtc gatgtcgctg tccggacgct gcgaaccgga
     3841 cgcggccgac ccgaagagga tcaggaggcg gacgtcccgc tcccggcaca ggcggacgag
     3901 gccgtcgcgg cgcgcggcca ggcgccgcac cacgtcgagc cgaccacgct cgagatccat
     3961 gcccatgacc tcgccatggt acgcgcgcgc cggcggacgg ccccgtacgg gggggtggcg
     4021 ccccccagga gcaaggacgc cttcggcacg ggtctcatct ccgcgccgcg ccgttccggc
     4081 ccggcgcgtc agctctcctg ctggccggcc atctcgacgg ggggcgtggc cggcggcgtg
     4141 ggggcgtgct cgaacgtgat ctcctcctcg gccacgtcca cgatcaccgt ctcgccggcg
     4201 gcgaactcct tccacaggag cttctcggag agcgggtcct cgaccatgcg ctggatcgtc
     4261 cggcgcagcg ggcgggctcc aagcgagggg tcgtacccct tctcggccag taggttcttg
     4321 gccggctcgg tcaggacgat gtccacgtcc ttcgacttca gctgctcctc gacccggcgc
     4381 atcatcaggt cgacgatcgc cttgacgtcc tcgcgggaca gcgagtggaa cacgatgacc
     4441 tcgtcgatcc ggttcaagaa ctctggtcgg aacgagcgct tcagctcatc ggtcaccttc
     4501 gccttcatct tgtcgtaggt gacctgctcg tcggcctgcg ccgcgaaccc gaccgcgatg
     4561 gtctgcaggt accgcgtccc gaggtacgag gtcatgatga tcacggtgtt cttgaagtcg
     4621 acctggtggc cctgggcgtc cgtcaggtgg ccgtcctcca ggatctgcag gagcgtgttg
     4681 aacacgtccg gatgcgcctt ctcgatctcg tcgaacagca ccacggagaa cggcttgcgc
     4741 cggacggcct cggtgagctg gccgccctcc tcgtacccga cgtagcacgg gggcgagccg
     4801 accagccggc tgaccgtgtg cttctccatg tactcggaca tgtccagctg gatcagcgcg
     4861 tcctcgtcgc cgaacaggaa ctcggcgagc gccttcgaca gctcggtctt cccgacgccg
     4921 gaggggccga ggaagatgaa cgagccggac gggcgcttcg ggtccttcag gccggcgcgg
     4981 gtgcggcgga tggccttcga caccgcggtg acggcctcga cctggtcgac gatccgcttg
     5041 tgcagctcgt cctccatccg caggagcttc tgggtctcct cctcggtcag cttgtagacc
     5101 gggatgccgg tccaggccga gagcacctcg gcgatctcct cctcgtccac ctcggccagc
     5161 acgtcgagct cgcccgactt ccattcctgc tcgcgctggg tgcgctcctc gcccaggcgg
     5221 cgctcctcgt cgcgcagccg cgcggcgccc tcgaagtcct gggcgtcgat cgaggactcc
     5281 ttctgccggc gcacgtcggc gagcttctcg tcgatctcgc gcacctcggg cggcgcggtc
     5341 atccggcgga tgcgcatccg gctgccggcc tcgtcgatca ggtcgatcgc cttgtcgggg
     5401 aggtagcggt cgctgatgta gcggtcggcc agattcgccg cggcgaccag cgcctcgtcg
     5461 gtgaacgaga cgcggtggtg cgcctcgtac cggtcgcgca gccccttcag gatgtcgatg
     5521 gtgtgcgcca ccgacggctc ctcgacgtag atcggctgga accggcgctc gagcgccgcg
     5581 tccttctcga ggtgcttgcg gtactcgtcc cgcgtggtgg cgccgatcgt ctgcagctcg
     5641 ccgcgcgcga gcatcggctt caggatgctc gccgcgtcga tggcgccctc ggcggcgcca
     5701 gccccgacga gcgtgtggag ctcgtcgatg aacaggatga tgtcgccgcg ggtcttgatc
     5761 tccttcagca ccttcttcag gcgctcctcg aagtccccgc ggtagcggct gccggcgacg
     5821 agcgcgccga ggtcgagcgt gtagatctgc ttccccttga tcgtctccgg gacctcgccc
     5881 ttcacgatcg cggaggccag gccctcgacg atggcagtct tgcccacgcc ggggtcgccg
     5941 atcaggaccg ggttgttctt ggtccgccgc gacaggacct gcatgacccg ctcgatctcc
     6001 ttctcgcggc cgatcaccgg gtcgagcttg ccctcgcggg cgttctgggt caggttgcgg
     6061 ccgaactggt cgagcacgag cgacccctgt ggggtctgct cgccgggctg cccctcgccc
     6121 ttgccgccgg tgtagccgga cagcagctgg atgacctgct ggcggacgcg gttgaggtcg
     6181 gcgccgagct tctgcagcac ctgcgcggcg acgccctcgc cctcccggat caggccgagc
     6241 aggatgtgct cggtgccgat gtagttgtgg ccgagctgca gcgcctcgcg cagcgacagc
     6301 tcgaggacct tcttggcccg cggggtgaac gggatgtgcc cggtgggcgc ggcctgcccc
     6361 tggccgatga tctcctcgac ctgctggcgc accgcttcga gcgagatgcc gagggactcg
     6421 agggccttcg cggcgacgcc ctcgccctcg tggatcagcc cgagcaggat gtgctcggtg
     6481 ccgatgtagt tgtggttgag gaggcgcgcc tcctcctgcg ccaggacgac gactcgccgg
     6541 gcccggtcgg tgaagcgctc gaacatccgt cacctcccgc cggccgagcc ggcccgttcg
     6601 tctcgtcccg gggatccgcg gaccacgtcc tacccccgag gcgcgggtgg cgcccccgtg
     6661 cgacgctcct cgtccttcgt cgcgcccccc ggagacgcct cctcgggagc gattataccg
     6721 gccccctccg atacgcccgg agcgccccgt cccatccggc ctccgggcgg cgccccatct
     6781 ctggtattcg gacgtttccc ggggcgagtt cagtttcaca cactgatttc tgccgccgtt
     6841 gcgggtgcgg tccgaccgtt tgacggggaa cggccgaagg cagagccttc ggcgaggagc
     6901 ggaacgaggg gcgagggggg atgtccgaac acgtcgatca gcaccacgag cacggcgggc
     6961 gggtccacgg tcacaagcac ttccacgtga cgcactacct ccgcacgggg gaggactggg
     7021 cgcacctgac cgccgtgcac gagcacgagc acaatcaccc gcagatcgcc cacacgcaca
     7081 agccacacga ggacgaggcc cacgagcaca cccgcgaggc ccacgtccac gaccacgccc
     7141 gtcccgcgga atcccccggg tagcggcccg ccgtcagtgc agcgggtcgt cggggcccgt
     7201 cgtgatgccg agcctcgagg atcggtactt cgggctgggg gccggcgggt gccaggccgg
     7261 ccagaagggg ccccggtccc gctccaggac gatcgtccgc tcgccgtcgg aggtgaacgt
     7321 gcgcatcacc ggccgccacg gggcgtcggg cacgagccac ggctcggaga cctcgtcccg
     7381 gccgggaacg gcgaaggccc cgagctgcag ttcgtcgacg aacgtgtggt cgtccggtcc
     7441 cagggccacc ggcaagg
//
LOCUS       DASPWO010000583         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_81592, whole genome shotgun sequence.
ACCESSION   DASPWO010000583 DASPWO010000000
VERSION     DASPWO010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81592"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..335
                     /locus_tag="VEO00_13305"
     CDS             168..335
                     /locus_tag="VEO00_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59006.1"
                     /translation="MVEIPYHEHRGEPLWLELQDFVEAVRSARLPLVDGHAGVAALAL
                     CQRILEAAKQD"
     gene            347..1417
                     /gene="wecB"
                     /locus_tag="VEO00_13310"
     CDS             347..1417
                     /gene="wecB"
                     /locus_tag="VEO00_13310"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HYU59007.1"
                     /translation="MHIAYLIGTRPEAIRSARILEILEADRDVRLTLISSGQHFDWNM
                     LPGFIHELSLPPVTIDLQVGRGSPADHVASILAGLAAAIRSDLPDALCVFGDTSSTLA
                     GGLAAALLEVPLVHIEAGCRSFDMSMPEEINRRLVDHAAGLLLAVSDVGRQNLVREAV
                     PGRIEVVGDPQYDVFAREVETLTPAAAESDERVGLVTLHRPSNVDDTKTLFGILDQLD
                     AAAREADLTWIFPVHPRTRRVLEEAPHRSIQHTEPLLYRDLLAILARASVCVTDSGGL
                     QKEALWMTVPCVTVRTTTEWLETLWQGTNVLAPPGSDIVASIVRSLREGDRDYSNPYG
                     DGRASERIVRVIKEWIGARTSR"
     gene            1756..2238
                     /locus_tag="VEO00_13315"
     CDS             1756..2238
                     /locus_tag="VEO00_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYU59008.1"
                     /translation="MALVEEGRPAIGVVHAPFLGRGETETYVGWAGGGAWRGRERLSV
                     SARHPGEAVVGTGFPFRHKEWLPRYLAAFGAAFERFEDLRRPGAADLDLAWVAAGVFD
                     GFFELGLGTWDVAAGALLIEEAGGVVSDWQGGPDYLGGDILAGPPVIHAALLEIARHG
                     "
BASE COUNT          329 a          792 c          839 g          362 t
ORIGIN      
        1 tgacggtcgc gtcggccggc ccgtcgctga ccggcaagca ggttaccctt ggctgtccgg
       61 tcgatcccgt tcggcgagga gagccataga tgcacgcacc gaggatcccc tccgcccgac
      121 gtcgaggtgc atccgacggt cttggtcgag gaggaggccc agatcaggtg gtggagatcc
      181 cgtaccacga gcaccggggc gagcctctgt ggctggagct ccaggacttc gtggaagccg
      241 tccgctcggc tcggctgccg ctcgtcgacg ggcacgcggg agtcgcggcc ctcgccctct
      301 gccagcggat cctcgaagcg gccaagcagg actgaccgag ccgaccgtgc acatcgcgta
      361 tctgatcgga accagacccg aggcgatccg ctctgcccgc atcttggaga tccttgaagc
      421 cgaccgcgac gtccgactca ccctaatctc gtccggccag cacttcgact ggaacatgct
      481 accggggttc atccatgagc tgtcgttgcc ccccgtaacg atcgacctgc aggtcggacg
      541 gggctccccc gccgatcacg tggcctcgat cctcgccggc ctcgccgccg ccatcagatc
      601 cgacctgccg gacgccctct gcgtcttcgg cgatacgagc tccacgctgg ccggcggcct
      661 ggcggctgcg ctcctcgaag tccccctggt ccacatcgag gccggctgcc ggagcttcga
      721 catgtccatg cccgaggaga tcaaccgccg actggtcgac cacgcggcgg gcctgctgct
      781 ggccgtctcg gacgtcggcc ggcagaacct cgtccgagag gccgtgccgg gccggatcga
      841 ggtggtaggc gacccccagt acgacgtctt cgcgcgtgag gtcgagacgc tcactcccgc
      901 cgcagccgag tcggacgagc gtgtcgggtt ggtcacgctc caccggccct ccaacgtgga
      961 tgacacgaag acgctcttcg gcatcctcga ccaactggac gccgcggcca gggaggcgga
     1021 cctcacgtgg atcttcccgg tccacccgcg aacgcgacgg gtgctggaag aggcccccca
     1081 ccgatccatc cagcacacag agcctctgct ctaccgagat ctgctcgcga tcctcgcgcg
     1141 cgcgtcggtc tgcgtgaccg attccggcgg ccttcagaaa gaggccctgt ggatgacggt
     1201 tccctgcgtg accgtccgga ccacgaccga atggctcgag acgctgtggc agggcaccaa
     1261 cgtattggcg ccacccgggt cggacatcgt cgcctcgatc gtccggagcc tgcgggaggg
     1321 ggatcgcgac tactcgaacc cgtacggaga tggccgagca tccgagcgga tcgtgcgcgt
     1381 catcaaggag tggatcggag ctagaacttc acggtgaagc cccagtgtcg cgtcgtagcg
     1441 tgcccggcgt gagcggtggc gcgagcctac cggagctggc cgaggcggcg gtcgcggcgg
     1501 cgctggcagg agggcgcgtc gtgggggcgc gaggcccgtc cggggtgacc ctgaaggcgg
     1561 ccggcgatta cgtctcggac gtcgaccgcg cttcggagga ggcggtcttg caggtccttc
     1621 gggcgggtgc gcccgagatc cccgtgctgg cggaggaggc cggcggcgag cgcaccgccg
     1681 agcgctattg ggccgtggac ccgctcgacg gcaccacgaa ctacctccac tcgttcccgg
     1741 ccgtcggagt ctcgggtggc gttggtggag gagggccgtc cggccatcgg cgtcgtgcac
     1801 gctccgttcc tgggtcgtgg cgagaccgag acctacgtcg ggtgggccgg gggcggcgcg
     1861 tggcgcggcc gcgagcggct gtctgtgtcc gcccgccacc ccggcgaggc cgtcgtcggc
     1921 accgggttcc cgttccgaca caaggagtgg ctaccccggt acttggcggc gttcggggcc
     1981 gcgttcgagc gcttcgagga cctgcgcagg cccggggcgg ccgacttgga cctggcgtgg
     2041 gtggcggccg gcgtgttcga cgggttcttc gagctcggcc tggggacgtg ggacgtggcc
     2101 gccggggcgc tgctcatcga ggaggcaggc ggcgtggtga gcgactggca gggagggccg
     2161 gactacctgg gcggcgacat actggccgga ccgccggtca tccacgcggc cctgttggag
     2221 atcgcgcggc acggctgacc ggtcccgagc tgggtctttc gtgatgggaa gggacgccgg
     2281 gctccaggcg atcgtgactc cttcggcaag cgctcgccga tg
//
LOCUS       DASPWO010000584         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_81594, whole genome shotgun sequence.
ACCESSION   DASPWO010000584 DASPWO010000000
VERSION     DASPWO010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81594"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..875)
                     /gene="sucD"
                     /locus_tag="VEO00_13320"
     CDS             complement(6..875)
                     /gene="sucD"
                     /locus_tag="VEO00_13320"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HYU59009.1"
                     /translation="MAVLVDRETRVVVHGITGDQGTFHALRNKAYGTRVVAGMTPGKA
                     GRHVDGIPVFDTVVEAVAETGANTSLIFVPARFAADAILEAGDAGVALIVCITEGIPV
                     MDMARVHGALRGRSPRLIGPNCPGVLSPGQANVGIIPWEIGSEGHVGMVSRSGTLTYQ
                     VMHELTRKGIGQSTCIGIGGDPIIGTGFVDALALFQEDPQTEAIVLIGEIGGSDEEEA
                     ATFIAEHVTKPVVAYVAGRTAPPGKRMGHAGAIITGSSGTAKAKAEAFEAVGVRVGRN
                     PTEVADLVEGVRP"
     gene            complement(875..2017)
                     /gene="sucC"
                     /locus_tag="VEO00_13325"
     CDS             complement(875..2017)
                     /gene="sucC"
                     /locus_tag="VEO00_13325"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HYU59010.1"
                     /translation="MDLLEYQGKELFARHGIPLLEGRVATSAAQARAAAEAFGGSAAV
                     KVQVPTGKRGKGGGIVLARSADEAEAAAARMFSEGFQGMPVTTVLVERLAELAGEFYA
                     AITLDRANRAYVAMTSTEGGMDVEEIAASRPEALRRAPVDPLLGMLVHTVRYLSGGFP
                     PDARAGVSEVLTRMWELLVANDATLVEINPLVLLADGSMVALDAKVTADDNALYRRPE
                     LAGYQAFSDPTEARAREAGLQYVKLDGEIGIIGNGAGLVMSTLDVVQQAGGAAANFLD
                     VGGGASADMMALSLEVVLSDPAVRSVLVNIFGGITRCDDVARGIVEALGRVEALVPIV
                     VRLDGTNAGEGRTILGEASHERIVPAETMLEGAERAVALAREGAGI"
     gene            complement(2020..>2238)
                     /locus_tag="VEO00_13330"
     CDS             complement(2020..>2238)
                     /locus_tag="VEO00_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HYU59011.1"
                     /translation="HSGAHMTLFPRVVRLLRERGAGEILVFGGGIIPAADVPGLKEAG
                     IEEIFTPGTPTTAIVEWLRERLGRRQPA"
BASE COUNT          296 a          875 c          759 g          308 t
ORIGIN      
        1 gcacctcacg gccgcacccc ctcgaccagg tcggcaacct cggtcgggtt gcgccccacc
       61 cgcacgccaa ccgcctcgaa cgcctccgcc ttcgccttgg ccgtgccgct cgatccggtg
      121 atgatcgccc cggcgtggcc catccgcttg cccgggggcg ccgtgcggcc ggccacgtag
      181 gcgaccaccg gcttggtgac gtgctcggcg atgaacgtcg ccgcctcctc ctcgtcggag
      241 ccgccgatct ccccgatcag cacgatcgcc tcggtctgcg ggtcctcctg gaagagggcc
      301 agcgcgtcga cgaaccccgt gccgatgatc gggtcgccgc cgatgccgat gcaggtggac
      361 tggccgatcc ccttgcgggt cagctcgtgc atcacctggt aggtgagcgt gccggagcgc
      421 gagaccatcc cgacgtgccc ctcgctgccg atctcccacg ggatgatccc gacgttcgcc
      481 tgccccggcg agagcacgcc ggggcagttc ggcccgatca gccggggcga cctgccgcgc
      541 agcgcgccgt gcacgcgcgc catgtccatc accgggatgc cctcggtgat gcacacgatc
      601 agcgcgacgc cggcgtcgcc agcctccagg atcgcgtcgg cggcgaagcg ggccgggacg
      661 aagatcagcg acgtgttcgc cccggtctcc gccaccgcct cgaccaccgt gtcgaacacc
      721 gggatgccgt cgacgtgccg tccggccttg ccgggggtca tgcccgcgac gacccgcgtg
      781 ccgtaggcct tgttgcgcag ggcgtggaac gtgccctggt ccccggtgat cccgtggacg
      841 acgacccgcg tctcgcggtc gacgaggacc gccatcagat ccccgctccc tcgcgggcca
      901 gcgcgacggc gcgctcggcc ccctcgagca tcgtctcggc cggaacgatc cgctcgtggc
      961 tcgcctcgcc caggatcgtc cggccctccc cggcgttcgt gccgtcgagg cggacgacga
     1021 tcggcaccag cgcctccacg cgcccgagcg cctcgacgat ccctcgcgcc acgtcgtcgc
     1081 accgcgtgat cccaccgaag atgttcacca gcaccgagcg gacggccggg tcggagagga
     1141 cgacctcgag tgagagcgcc atcatgtcgg cgctcgcgcc gccgccgacg tcgaggaagt
     1201 tggcggccgc gcccccggcc tgctgcacca cgtcgagcgt ggacatcacc aggccggcgc
     1261 cgttcccgat gatcccgatc tcgccgtcga gcttcacgta ctggagtccc gcctcccggg
     1321 cccgggcctc ggtggggtcg gagaacgcct gatagccggc cagctcgggc cggcggtaga
     1381 gcgcgttgtc gtccgccgtg accttggcgt cgagggccac catgctcccg tcggccagca
     1441 gcaccagcgg attgatctcg acgagggtcg cgtcgttcgc caccagcagc tcccacatcc
     1501 gcgtcaggac ctccgacacc ccggcgcggg catcgggggg gaagccgccc gacaggtagc
     1561 gcacggtgtg gacgagcatc ccgagcagcg ggtcgaccgg cgcccggcgc agggcctccg
     1621 gacgggaggc cgcgatctcc tcgacgtcca tgcccccctc cgtggaggtc atcgccacgt
     1681 aggcccggtt ggcccggtcg agcgtgatcg ccgcgtagaa ctccccggcg agctcggcca
     1741 gccgctcgac cagcaccgtc gtcaccggca tcccctggaa gccctcggag aacatccgcg
     1801 cggcggccgc ctcggcctcg tcagccgagc gggcgagtac gatgccgccc cccttgccgc
     1861 gctttccggt cgggacctgc accttgacgg cggcgctgcc gccgaacgcc tcggccgcgg
     1921 cgcgcgcctg cgcggcggac gtcgccaccc ggccctcgag gagcgggatc ccgtggcggg
     1981 cgaagagctc cttcccctgg tactcgagca ggtccatggc tacgcgggct ggcgccgccc
     2041 gaggcgctcg cgaagccatt cgacgatggc ggtggtgggc gtgccggggg tgaagatctc
     2101 ctcgatcccc gcctccttca gccccgggac atcggcagcc gggatgatcc cgcccccgaa
     2161 caccaggatc tcccccgcgc cgcgctccct gagcagcctg accacgcggg ggaagagcgt
     2221 catgtgggcg ccggagtg
//
LOCUS       DASPWO010000585         8669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_163081, whole genome shotgun sequence.
ACCESSION   DASPWO010000585 DASPWO010000000
VERSION     DASPWO010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163081"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VEO00_13335"
     CDS             complement(<1..278)
                     /locus_tag="VEO00_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU59012.1"
                     /translation="MSLRFGVSVPNVGDLDALVALGVEADRAGWDGFFLWDHMAFTRE
                     FPVPVFDPWVTLGALAVQTERIRLGTMVTPLARRRPWKLARETVTLDHL"
     gene            complement(275..640)
                     /locus_tag="VEO00_13340"
     CDS             complement(275..640)
                     /locus_tag="VEO00_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59013.1"
                     /translation="MGLDSFAARSPEGGLTAEDERAFAAAEIDLCGGMYSDGTVSFRG
                     KVYSTAIEPVSGVSIYQRWIAPPELRAIAEAFDRCDPETVERWMADEPDRTTAEEVLQ
                     LRAFFDICRDRGLGLVGWW"
     gene            complement(646..3453)
                     /locus_tag="VEO00_13345"
     CDS             complement(646..3453)
                     /locus_tag="VEO00_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HYU59014.1"
                     /translation="MSPDPAHPSGAVAGAADGGATVPGVLSSVAAASGQDALALLRSS
                     DDGLSPDEAAARLARVGPNELPRPHGPGLVRQLLDQLVHFFALMLWAAAALAFVGGMP
                     QLGVAIIVVVLVNGAFSFAQEFRAERAVRALAALLPEVAVVRRGGRRLTVPAGALVPG
                     DIVLLAEGVRISADARVLHSSELRVDVSTLTGESKPVARVTEPVAEPPRDPLEAPNVV
                     FAGTFVTSGSGAAVVAATAGATRLGGISALTAGVVRRPTPLRIQLNRAVRVIGGFAVA
                     TGLGFFGIALALGTPARDGFLFSVGVIVALVPEGLLPTLSLSLAMSATRMARGGALVR
                     RLESVETLGSTTVICTDKTGTLTTNEMTVKAVVLPHGRFAVTGTGYDPGGTIVTPEGR
                     PLDDEQWADVRRLLHTAALCGDARVEARDGRWRCLGDPTEGALLVLARKGGVERAAAE
                     RAAPRVREFPFESRRQRMSTVHILPSGAYEVLAKGSPEAVLPLCKSARVDSVAPPLGE
                     DEHRDIMAAVDSLAVKGLRVLAFARREIEGGVPETAREAEQEMEFLGLVGMADPVRPE
                     VPDAVARCRRAGIRLVVITGDHPATAMSIAREAGIPAHTVMLGSELPVNDERLGALLG
                     THEVVLARVAPEEKLRIARALQARGEVVAMTGDGVNDAPALRQSDIGVAMGKVGTDVA
                     REAADLVLLDDNFAHIVQAVEEGRAAFDNIKRFLTYHLTDNVAELTPFVAWALSAGAI
                     PLMISVLQILALDIGTDLLPALALGAEKPEPGVMARPPRARSARLLDGRVLGRAFGFL
                     GPVEAAGAMAMIVFGAALFFGWPGQPLPDTGADKAVLSTMVFAAIVAMQMANAFECRS
                     TPASLHSIGPFTNRLLVWAVGGELLALSAFVYLPPLADALGQRALGPAQWLPVAVTPL
                     LLLAAEEGRKALVRRRADRPG"
     gene            complement(3450..4373)
                     /locus_tag="VEO00_13350"
     CDS             complement(3450..4373)
                     /locus_tag="VEO00_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59015.1"
                     /translation="MDALLVVLVLLGGVLLLALFALARVLLPARPPAEAPPAVLIERR
                     AVVTLDVGPADPADPAAERLVHEAAARVFAAIPDAEEVEVRARDGSVLGRTTRVVPES
                     RPISLPEYLHEPHRPRHRGPDLAGRQAEEERLAPPARQAGEAGTAVPVEPAPGERRPF
                     AERFELPPAIRDGVRDPNDPVDVVRAIFAAAGLAVRVDGDLLRVEDLAIAVVPVEGGA
                     RDTLNRAYRWIRAAGPGRGLVVALGAVDPDEVRRREMLDPSVLHTGLSGVQRMADAVA
                     LGADPVRFAVAPALAPASLPAERRPPRRQSR"
     gene            4517..4861
                     /locus_tag="VEO00_13355"
     CDS             4517..4861
                     /locus_tag="VEO00_13355"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYU59016.1"
                     /translation="MLTGERALSATDVEARPWRPFAGQPGVRDKVLWEDPATGSYAGM
                     MEIEPGATVRTHGHRRAVHHLFVVRGSCELAVTERVLGPGAYTFVPAATKHGIESAGT
                     EGCTLFFLYLRP"
     gene            4871..6208
                     /locus_tag="VEO00_13360"
     CDS             4871..6208
                     /locus_tag="VEO00_13360"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HYU59017.1"
                     /translation="MGSALLTDLYELNMAASYLRRGMVGPATFSLFVRRLPPTRGFLA
                     AAGLEDCLAFLEGLRFEEEDLRYARSELGFGEQDVRAFADLRFTGEVWAVPEGTIVLA
                     NEPILEVTAPIAEAQLAETFLLNQVTFQTTVASKAARCLLAAGGRDLVDFSFRRTQGV
                     EAAMAVARATAIAGFVATSNVEAARRFGLRAAGTMAHSFVEAFPTEEQAFRAFAEDFP
                     GRTTFLVDTYDTLGGVRAAIETIRALGLSEGLGIRLDSGDLEALARGSRALLDEAGLP
                     AVRIFASGGLDELEIDELVRGGAPIDAFGVGTRVGVSDDAPSLDSAYKLVRFGERPVL
                     KLSPEKATAPGAKQVFRSEGLRDAIGLRDEPVPAGARPLLEPVMAGGRRLRPPEPLAA
                     ARERLAVDLAALPDEARDLRSPRAPAVPFTPAARDLLERARGERVRAPSAEAR"
     gene            6205..6672
                     /locus_tag="VEO00_13365"
     CDS             6205..6672
                     /locus_tag="VEO00_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59018.1"
                     /translation="MTDMADQARPVLAAVRGRRHDLEAVLGQVERALAAPGAGRAGRW
                     LAGVRSELSHLREGFARHVEITEGSGGLHEEIVRRAPRLAPMVARLQDDHAAIGRAIE
                     ERLAAIPEDAGDDERVADTREAVLALLARLTRHRQSGADLIYEAYSVDIGSGD"
     gene            complement(6703..6924)
                     /locus_tag="VEO00_13370"
     CDS             complement(6703..6924)
                     /locus_tag="VEO00_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HYU59019.1"
                     /translation="MRAHVGDRIVVASTQVGQDPREGEILEVTEGPEGSEYRVRWADG
                     HESLFRPKAGIAKVVAAARPKAARTKKKG"
     gene            complement(7050..>8669)
                     /locus_tag="VEO00_13375"
     CDS             complement(7050..>8669)
                     /locus_tag="VEO00_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HYU59020.1"
                     /translation="WLEMRAVGQASRALEALASLLPDEAERVREDGVETVPLEALQPG
                     DVVLIRSGGRVPADGEVVEGSAALDESMITGESKAVSKSPGDPVVGGTVVTDSAIRAR
                     VTAVGEDTALAGIRRLVDEAQRSRSRAQVLADRAAAILFYVASGVGVVTYVVWTLLGE
                     QRDAIERTVTVLVIACPHALGLAIPLVIAISTGLSARHGILVKDRLALERMRKVDVVL
                     FDKTGTLTSGRPGVSGLVTVAGDDEDLLRLAAAVEADSEHPLARAIVAAAGARGAPLP
                     RATSFRSLTGRGVEASVDGARVAVGGPSFLRERDLAVPDAIAPSVKEWEERGATVLYV
                     SRDDTVVGAVALEDQVRAESRQAVGALKDIGLEVAMITGDARPVAEAVARQLAIQQVF
                     AEVLPENKDQAVADLQSRGKQVAMVGDGVNDAPALARADVGIAIGAGTDVAIESAGVV
                     LASDDPRAVVDVIRLSRASYRKMVQNLAWATGYNLVALPVAAGVLAWAGVTLAPAAGA
                     VLMSISTIVVALNAQVLRRLKLGASAGRAAT"
BASE COUNT         1160 a         3333 c         3141 g         1035 t
ORIGIN      
        1 aggtgatcga gggtgacggt ttcgcgggcg agcttccagg gccggcggcg ggccagcggc
       61 gtgaccatcg tgcccaggcg gatccgctcc gtctggaccg ccagcgcgcc gagcgtcacc
      121 cacggatcga acaccggcac cgggaactcc cgggtgaagg ccatgtggtc ccagaggaag
      181 aacccgtccc accccgctcg atcggcctcc accccgagcg cgacgagcgc gtcgaggtcg
      241 ccgacgttcg gcacgctgac cccgaagcgc aggctcacca ccaccccacc aggccgaggc
      301 cgcggtcgcg gcagatgtcg aagaaggcgc gcagctggag gacctcctcg gcggtggtcc
      361 ggtcgggctc gtcggccatc catcgctcca ccgtctccgg gtcgcaccga tcgaaggcct
      421 cggcgatcgc gcggagctcg ggcggcgcga tccaccgctg gtagatgctc acgccgctga
      481 ccggctcgat ggcggtcgag tagaccttcc cgcggaacga gaccgtgccg tcgctgtaca
      541 tcccgccgca caggtcgatc tcggcggccg cgaaggcccg ctcgtcctcc gccgtcagcc
      601 cgccctccgg cgagcgcgcc gcgaagctgt cgagccccat cgcgatcagc ccgggcgatc
      661 ggcacggcgc cggaccagtg cctttcgccc ctcctccgcc gcgagcagca acaggggggt
      721 gacggcgacc ggcagccatt gcgcgggccc gagcgcgcgc tgcccgagcg cgtccgcgag
      781 cggcggcagg tagacgaacg ccgagagcgc caggagctcg ccgccgaccg cccacaccag
      841 cagccggttc gtgaacgggc cgatcgagtg aagcgacgcc ggcgtcgacc ggcactcgaa
      901 ggcgttcgcc atctgcatgg cgacgatcgc ggcgaagacc atggtcgaga ggaccgcctt
      961 gtccgcgccg gtgtccggga gcggctggcc cggccagccg aagaacagcg ccgccccgaa
     1021 gacgatcatg gccatggcgc ccgcggcctc gaccggcccg aggaacccga acgcccggcc
     1081 gaggacgcgg ccgtccagga ggcgggcgct ccgcgcccgg ggcggccggg ccatcacccc
     1141 gggctccggc ttctcggccc cgagcgccag cgccggcagc aggtcggtcc cgatgtccag
     1201 ggccaggatc tggagcaccg agatcatcag cgggatcgcc ccggccgaca gggcccaggc
     1261 gacgaacggg gtgagctccg ccacgttgtc ggtcaggtgg taggtcagga accgcttgat
     1321 gttgtcgaag gcggcccggc cctcctccac ggcctgcacg atgtgggcga agttgtcgtc
     1381 caggaggacc aggtcggcgg cctcccgggc cacgtcggtc cccaccttcc ccatcgccac
     1441 tccgatgtcg gactggcgca gcgccggcgc gtcgttcacg ccgtcgccgg tcatcgccac
     1501 cacctcgccc cgcgcctgca gggcgcgggc gatgcgcagc ttctcctcgg gcgccacgcg
     1561 cgccagcacc acctcgtgcg taccgagcag cgcgccgagg cgctcgtcgt tcaccggaag
     1621 ctccgagccg agcatcacgg tgtgcgccgg gatgccggcc tcccgggcga tcgacatcgc
     1681 ggtggccggg tggtcgccgg tgatcacgac caggcggatc ccggcgcgcc ggcagcgcgc
     1741 caccgcatcg gggacctcgg ggcggaccgg gtcggccatc ccgaccagcc cgaggaactc
     1801 catctcctgc tcggcctccc gtgccgtctc cggcactccg ccctcgatct cccgccgggc
     1861 gaaggccagc acccgcaggc ccttcaccgc cagggagtcc acggccgcca tgatgtcgcg
     1921 gtgctcgtcc tcgccgagcg ggggggcgac cgagtcaacc cgcgcgctct tgcacagcgg
     1981 aaggaccgcc tccggcgagc ccttggccag cacctcgtac gcgccggacg gcaggatgtg
     2041 gaccgtgctc atcctctggc gccgggactc gaacgggaac tcccggaccc gcggtgccgc
     2101 gcgctccgcg gccgcccgct cgaccccgcc cttgcgcgcc aggaccagga gcgcgccctc
     2161 ggtcgggtcg ccgaggcagc gccatcggcc gtcccgcgcc tccacccgcg cgtcgccgca
     2221 gagcgccgcg gtgtgcagca ggcggcgaac gtcggcccac tgctcgtcgt cgagcggccg
     2281 gccctcgggg gtcacgatcg tcccgcccgg gtcgtagccc gtcccggtga ccgcgaaccg
     2341 cccgtgcggc aggacgacgg ccttcaccgt catctcgttc gtcgtcagcg tcccggtctt
     2401 gtccgtgcag atcacggtgg tcgagccgag ggtctccacc gactccagcc ggcggaccag
     2461 ggcaccgccg cgggccatcc gcgtggcgct catcgccagg gagagcgaga gggtgggcag
     2521 caggccctcc gggaccaggg cgacgatcac ccccaccgag aacaggaacc cgtcccgggc
     2581 cggcgtgccc agcgccaggg cgatcccgaa gaagcccagg ccggtggcga cggcgaagcc
     2641 gccgatcacc cgcacggccc ggttgagctg gatccgcagc ggggtcggac gccggaccac
     2701 gccggccgtc agcgccgaga tgccgcccag ccgggtcgcg cccgcggtgg ccgccacgac
     2761 ggcggcgccc gacccggagg tgacgaaggt ccccgcgaac acgacgttcg gggcctccag
     2821 ggggtcgcgg ggcggctcgg ccacgggctc cgtcacccgg gcgaccggct tcgactcgcc
     2881 ggtcagcgtg gacacgtcga ccctcagctc cgacgagtgg aggacgcgcg cgtcggcgga
     2941 gatccggacc ccctcggcca gcagcacgat gtcaccggga acgagggcgc cggcggggac
     3001 cgtcagcctc cgaccgccgc gccggaccac ggccacctcc ggcaggagcg ccgccagggc
     3061 gcgcacggcc cgttccgccc ggaactcctg ggcgaagctg aatgccccgt tcaccaggac
     3121 gacgacgatg atcgcgacgc cgagctgggg catgcccccg acgaacgcga gcgcggcggc
     3181 cgcccacagc atcagcgcga agaagtgcac cagttggtcg aggagctgcc ggaccaggcc
     3241 cgggccgtgc ggccggggca gctcgttcgg gcccacccgg gcaaggcggg cggcagcctc
     3301 gtccggcgac agcccgtcgt ccgagcttcg caggagcgcc agcgcgtcct gcccgctcgc
     3361 cgccgcgacg ctcgacagca ccccgggcac cgtcgcgccg ccgtcggcgg ccccggcgac
     3421 cgcgccggac ggatgcgccg ggtccgggct cacctgctct gacggcgcgg cggacgccgc
     3481 tcggcgggaa ggctggccgg cgcgagcgcc ggggcgaccg cgaagcggac cggatccgcg
     3541 ccgagcgcga cggcgtcggc catgcgctgc acgccggaca ggccggtgtg cagaacggag
     3601 gggtcgagca tctcccgccg gcgaacctcg tcgggatcga cggcgccgag ggcgacgacc
     3661 agcccgcgcc cggggccggc cgcccggatc cagcggtagg cgcggttcag cgtgtcgcgc
     3721 gcgccgccct caaccggcac cacggcgatc gccaggtcct cgacccggag caggtcgccg
     3781 tcgacccgca ccgccaggcc cgcggcggcg aagatggcgc ggacgacgtc gacgggatcg
     3841 ttcgggtcgc ggacgccgtc ccggatcgcc ggggggagct cgaagcgctc cgcgaacgga
     3901 cgccgctccc ccggcgccgg ctcgacgggc accgccgtcc cggcttcgcc ggcctggcgg
     3961 gcgggcggcg cgagccgctc ctcctccgcc tggcgtcccg cgaggtcggg cccgcggtgc
     4021 cgcggccggt gcggctcgtg caggtactcg ggcagtgaga tcggccggga ctcggggacc
     4081 acgcgcgtcg tgcggccgag cacgctcccg tcgcgcgcgc ggacctccac ctcctcggcg
     4141 tcggggatcg ccgcgaacac cctggcggcc gcctcgtgga ccagccgctc cgcggcgggg
     4201 tccgcggggt ccgcgggacc cacgtcgagc gtcacgaccg cccggcgctc gatgaggacg
     4261 gccggcgggg cctccgctgg cggccgcgcc ggcagcagca cccgcgccag cgcgaacagg
     4321 gcgagcagca gcacgccgcc cagcaggacc agcaccacca ggagcgcgtc cacgatcgca
     4381 gcgtagccga gagattcccg ccgttcaccg ggtcgggcga cccaccgatt ggtggaccag
     4441 cgggactgcc cggtccgggc agccggaggg atgctttccc cgtcgggccc gcccgacgac
     4501 acgggaggtg atcaggatgc tgacgggaga gcgcgcgctg agcgcgaccg acgttgaggc
     4561 gcgtccctgg cggccgttcg ccggccagcc gggggttcga gacaaggtgc tgtgggagga
     4621 cccggccacg gggtcgtacg ccgggatgat ggagatcgag ccgggcgcca cggtccgcac
     4681 gcacgggcac cgccgggccg tccaccacct gttcgtggtg cgcgggtcct gcgagctcgc
     4741 ggtgaccgag cgcgtgctcg gccccggggc gtacacgttc gtccccgccg cgacgaagca
     4801 cgggatcgag agcgctggga ccgagggctg tacgctcttc ttcctctacc tccggccctg
     4861 acgggaggcc atgggcagcg cgctgctcac cgatctctac gagctgaaca tggcggcgag
     4921 ctacctccgc cgcgggatgg tggggcccgc cacattcagc ctcttcgtgc gccgtctgcc
     4981 tccgacgcgg ggcttcctcg ccgccgccgg actggaggac tgcctggcgt tcctggaggg
     5041 cctccggttc gaggaggagg acctccggta cgcgcgctcg gagctggggt tcggtgagca
     5101 ggacgtccgc gcgttcgccg accttcgctt caccggggag gtctgggcgg tacccgaggg
     5161 gacgatcgtc ctcgcgaacg aaccgatcct ggaggtcacc gcgccgatcg ccgaggctca
     5221 gctcgcggag acgttcctcc tcaaccaggt cacgttccag acgaccgtcg cctcgaaggc
     5281 ggcgcggtgc ctgctggcgg ccggcggccg ggacctcgtg gacttctcgt tccggcggac
     5341 ccagggcgtc gaggccgcca tggccgtggc gcgggccacg gccatcgccg gcttcgtggc
     5401 cacgagcaac gtggaagccg cacgtcggtt cgggctgcgg gccgccggca cgatggcgca
     5461 ttccttcgtc gaggccttcc ccaccgagga gcaggcgttc cgggcattcg ccgaagactt
     5521 ccccggccgg acgacgttcc tggtggacac ctacgacacg ctcggaggcg tgcgtgccgc
     5581 gatcgagacg atccgcgcgc tcggcctttc ggaggggctc ggcatccgcc tcgacagcgg
     5641 ggacctcgag gcgctggcgc gagggtcgcg tgcgctgctc gacgaggccg gcctccccgc
     5701 ggtccggatc ttcgccagcg gcggcctgga cgagctcgag atcgacgagc tggtccgcgg
     5761 cggcgcgccg atcgacgcct tcggggtcgg tacccgggtg ggcgtgtccg acgacgcgcc
     5821 gtccctggac tcggcctaca agctggtgcg cttcggtgag cgtccggtgc tcaagctctc
     5881 cccggagaag gcgacggcgc cgggcgcgaa gcaggtgttc cgctcggagg ggctgcgcga
     5941 cgcgatcggc ctgcgggacg agccggtgcc ggcgggcgcc cgtcccctcc tggaaccggt
     6001 gatggccggc gggcggcggc tgcgaccgcc cgagccgctc gcggccgcgc gagagcgcct
     6061 ggccgtggac cttgccgcgc tgcccgacga ggcccgggac ctccgttcgc cccgcgcgcc
     6121 ggccgtgccg ttcacaccgg ccgcgcggga cctcctggag cgggcccgcg gcgagcgcgt
     6181 ccgagcgccg agcgccgagg cgcggtgacg gacatggcgg accaggccag gccggtcctg
     6241 gcggccgtcc gcggccggcg gcatgacctc gaggcggtcc tggggcaggt cgagcgcgcc
     6301 ctcgccgcgc ctggggcggg ccgggccggc cggtggctgg cgggcgtgcg atccgagctg
     6361 tcccacctgc gcgagggctt cgccaggcac gtcgagatca ccgagggctc gggcggcctg
     6421 cacgaggaga tcgtgcggcg cgcgccgcgg ctcgccccga tggtcgcccg cctgcaggac
     6481 gaccatgccg ccatcggccg ggccatcgag gagcggctgg ccgccatacc ggaggatgcc
     6541 ggcgacgacg agcgcgtcgc cgacacccgc gaggcggtgc tcgcgctcct ggcacggctc
     6601 acccggcacc ggcagtccgg ggccgacctg atctacgagg cctacagcgt cgacatcggc
     6661 tcgggagact gagcctcgga accgcgccgt cccagctcgg attcagccct tcttcttcgt
     6721 acgagccgcc ttcggacgag ccgccgcgac gaccttcgca atgccggcct tcggcctgaa
     6781 caggctctcg tggccatcgg cccagcggac gcggtactcg gaaccctcgg ggccttcggt
     6841 cacctcgagg atctcgccct ctcgagggtc ctggcccacc tgcgtggacg ccacgacgat
     6901 ccggtctccg acgtgggcgc gcatctcctc ccctcctcga tcgcttccga tccccaggct
     6961 ctcgccccca gggtagtgct cccggtcccc cccggggggg gcccgccaca ttgagatgac
     7021 gcgcacctgg agggcgaccg cctcctccgc tacgtggcgg cgcggcccgc cgacgcgccc
     7081 agcttcagtc gcctcagcac ctgcgcgttc agcgcgacca cgatggtgga gatgctcatc
     7141 aggaccgcgc cggccgcggg ggcgagcgtc acgccggccc acgccagcac gcccgcggcg
     7201 acgggcaacg ccaccaggtt gtacccggtc gcccaggcga ggttctgcac catcttccgg
     7261 tagctcgcac ggctgagtcg gatgacgtcc acaacggctc ggggatcgtc ggacgccagc
     7321 accacgccgg ccgactcgat cgcgacgtcc gtgcctgcac cgatcgcgat ccccacgtcc
     7381 gcccgggcca gggcgggcgc gtcgttcacg ccgtccccga ccatggccac ctgcttgccc
     7441 cgggactgga ggtcggcgac ggcctggtcc ttgttctccg ggagcacctc cgcgaagacc
     7501 tgctggatgg ccagctgccg ggcaaccgcc tcggccaccg gccgagcgtc cccggtgatc
     7561 atggcgacct cgaggccaat gtccttgagc gcgccgacgg cctggcgcga ctccgctcgc
     7621 acctgatctt cgagcgccac cgccccgacg accgtgtcgt cacggctgac gtacagaacc
     7681 gtcgctccgc gctcctccca ctccttgacc gagggtgcga tcgcgtccgg caccgcgagg
     7741 tcccgttcgc gcaggaacga ggggccgccg acggcgaccc gagccccgtc gacggaggcc
     7801 tccaccccgc gcccggtgag cgagcggaag gacgtcgcgc gaggaagggg ggcgccacgc
     7861 gcaccggcgg ccgcgacgat cgcccgagcg aggggatgct cgctgtccgc ctcgaccgcg
     7921 gcggccagcc gaagcaggtc ctcgtcgtcg ccggccaccg tgaccagccc ggagacgccg
     7981 ggacggcccg aggtgagggt gccggtcttg tcgaagagca ccacgtccac cttgcgcatc
     8041 cgctcgagcg ccagtcgatc cttcaccagg atgccgtggc gggccgacag accggtcgag
     8101 atggcgatca cgagggggat cgccagcccg agcgcgtgcg ggcaggcgat gacgagcacg
     8161 gtcacggtcc gttcgatggc gtcccgctgc tccccgagca gcgtccacac gacgtacgtg
     8221 accacgccca cgccggaggc cacgtagaag aggatcgccg ccgcccgatc ggccagcacc
     8281 tgcgcccggg agcgggaccg ctgcgcctcg tcgaccagcc ggcggatccc ggccagcgcc
     8341 gtgtcctccc ccaccgcggt gacgcgcgcg cggatcgcgg agtccgtgac gaccgtgccc
     8401 ccgaccaccg ggtcgccggg ggacttcgag accgccttcg actcgccggt gatcatcgac
     8461 tcgtcgagag ccgccgaacc ctcgacgacc tcaccgtccg ccggcacgcg gcctcccgac
     8521 cggatcagga cgacgtcgcc cggctgcagc gcctcgagcg ggaccgtctc gacgccgtcc
     8581 tccctgacgc gctcggcctc atcggggagg aggctcgcca gcgcctccag cgcccggctc
     8641 gcctgcccga ccgcgcgcat ctcgagcca
//
LOCUS       DASPWO010000586         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_505112, whole genome shotgun sequence.
ACCESSION   DASPWO010000586 DASPWO010000000
VERSION     DASPWO010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505112"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VEO00_13380"
     CDS             complement(<1..361)
                     /locus_tag="VEO00_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59021.1"
                     /translation="MDAVTRALGDEERPAVDPVAVAWDGEGGSVRIAGRAERASIEIA
                     LEDGGRVAVRVPVERGAAALPGGLPFGYHRTRVTAGRASGEVLIIAAPRRSYAGGRRL
                     WGVFLPLYAARSGRDWGA"
     gene            complement(433..>2897)
                     /locus_tag="VEO00_13385"
     CDS             complement(433..>2897)
                     /locus_tag="VEO00_13385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HYU59022.1"
                     /translation="LFMASRMEDRFPIIDILQQTPSIPDGAQWAVFLRNHDELTLEMV
                     TDEERDYMYRAYATDPQARINLGIRRRLAPLIGNDRRTMELMKALLFSMPGTPVVYYG
                     DEIGMGDNIYLGDRNGVRTPMQWSGDRNAGFSRANPQRLFLPVIIDPEYHYEAINVEA
                     QQANPHSLLWWMKRVIALRHRSRALSRGTLEFLFPENRKVLAFVRTYEDERVLVVANL
                     SRLAQHVEIDLSRFAGMVPVEVFGGTEFPQIGELPYLITLGPHSFYWLSLTAPRVEVP
                     LPAEESLPSLGAEGAWTGIVERHTRRGLERAIAAWLPTRRWFGGKGRSLKAVRVTDVV
                     PMGSSAGATRDDWLFMLVAVEYPDGDPETYVVPLAFRAGDDIPWSAIARVHSDDGDGV
                     LYDAFDDPAFGAALLASIGARRRFRGPTGRLVAQPTRAFRAMRGADDLTPTPVTAEQS
                     NTSLVYGERLVLKLIRRTEPGVNPDLEIGRFLTERTTFRDFPPVGGSLEFENGAGTPM
                     TVGVLQGFVPNEGDAWRYTLEELGRFIERALALGPEAEAPIPSGKTLIDLADAEPPRV
                     VVDTIAGYLESARLLGLRTAELHRALASDTEDPAFAPEPLGALYQRSVYQTMRATARR
                     AFQLARSRVREIPDLEEVLAYEDEALARFRRLLARKVDAQRIRCHGDFHLGQVLWTGR
                     DFVIIDFEGEPARALSERRIKRSPLTDVAGMLRSFHYATFTALLGQTGGGVVRPEDRA
                     AAEPWMHAWYLWTSAAFLRAYWEASGEGAWLPRTREDLEVLLDTFMLEKALYELRYEI
                     NNRPDWVPVPIRGVVQLLEGED"
BASE COUNT          413 a         1063 c         1025 g          396 t
ORIGIN      
        1 cggcgcccca gtcgcgccct gaccgcgccg cgtagagcgg gaggaagacg ccccacaggc
       61 gccgcccgcc cgcgtaggag cggcgaggcg ccgcgatgat cagcacctcg cccgatgcgc
      121 gtcccgcggt gacccgcgtc cggtggtagc cgaacgggag gccgccgggc agggccgcgg
      181 cgccgcgctc caccggcacc cggaccgcca cccgcccgcc gtcctccagt gcgatctcga
      241 tcgacgcccg ctccgcccgg ccggcgatcc gaaccgaccc cccctcgccg tcccaggcca
      301 cggccaccgg atcgaccgcc ggccgctcct cgtcgccgag cgctcgcgtc accgcgtcca
      361 cggccgcacg gggtgcccgc cgccgccggt cgaagccgtc gacgtaggcc gcctggatgc
      421 ccgagggggc ggtcagtctt cgccttccag caactgcacc accccgcgga tcgggaccgg
      481 gacccagtcg gggcggttgt tgatctcgta gcgcagctcg tagagcgcct tctcgagcat
      541 gaaggtgtcg aggagcacct cgaggtcctc gcgcgtcctc gggagccagg cgccctcgcc
      601 gctcgcctcc cagtacgcgc ggaggaaggc cgcggaagtc cagaggtacc aggcgtgcat
      661 ccacggctcc gccgcggccc ggtcctccgg gcggaccacg ccgccgccgg tctggccgag
      721 cagcgcggtg aaggtggcgt agtggaacga gcggagcatc ccggcgacgt cggtcagcgg
      781 cgagcgcttg atccgtcgct cggagagcgc cctcgccggc tcgccctcga agtcgatgat
      841 cacgaagtcc cgtccggtcc agagcacctg ccccaggtgg aagtcgccgt ggcagcggat
      901 ccgttgcgcg tcgaccttcc gtgccagcag gcggcggaag cgcgccaggg cctcgtcctc
      961 gtaggccagg acctcctcga ggtccgggat ctcccgtacc cgggagcggg cgagctggaa
     1021 cgcccggcgg gccgtggcgc gcatcgtctg gtagaccgag cgctggtaca gcgcgccgag
     1081 gggctccggt gcgaaggccg ggtcctccgt atccgaggcg agcgcgcggt gcagctcggc
     1141 ggtccgcagc ccgagcagcc gcgccgactc caggtagccc gcaatggtgt cgacgaccac
     1201 cctcggtggc tcggcgtctg ccaggtcgat cagggtcttc cccgacggga tcggcgcctc
     1261 cgcctccggg ccgagcgcga gcgcccgctc gatgaaccga ccgagctcct ccagcgtgta
     1321 ccgccaggcg tcgccctcgt tcgggacgaa gccctggagc acgccgacgg tcatcggcgt
     1381 cccggcgccg ttctcgaact cgagcgagcc tccgaccggc gggaaatcgc ggaaggtcgt
     1441 gcgttcggtg aggaaccggc cgatctccag gtcggggttg acgcccggct cggtccgccg
     1501 gatgagcttg agcaccagcc gctcgccgta cacgagcgat gtgttggact gctcggccgt
     1561 caccggcgtc ggcgtcagat cgtcggcgcc tcgcatcgcg cggaacgccc gggtcggctg
     1621 cgccacgagc ctgccggtcg ggccgcgaaa gcgccggcgc gcgccgatcg aggcgaggag
     1681 cgccgccccg aaggccgggt cgtcgaacgc gtcgtagagc acgccgtccc cgtcgtcgct
     1741 gtgcactcgc gcgatcgcgc tccacgggat gtcgtccccg gcccggaacg ccagcgggac
     1801 gacgtaggtc tcggggtccc cgtccgggta ctccacggcg accagcatga acagccaatc
     1861 gtcgcgcgtc gccccggccg acgagcccat cggcacgacg tcggtgaccc gcacggcctt
     1921 gagcgaacgc cccttcccgc cgaaccagcg cctcgtcggc agccaggcgg cgatggcgcg
     1981 ctcgagcccg cgccgggtgt gtcgctcgac gatccccgtc caggccccct cggcccccag
     2041 ggagggcaga ctttcctcgg cggggagcgg cacctcgacc cggggtgccg tcagcgacag
     2101 ccagtagaac gagtgcggcc cgagcgtgat caggtacggg agctcgccga tctgtgggaa
     2161 ctcggtcccg ccgaacacct cgaccggaac catcccggcg aagcgcgaca ggtcgatctc
     2221 gacgtgctgt gccagccgcg acaggttcgc gacgaccagg acccgctcgt cctcgtacgt
     2281 gcggacgaac gcgagcacct tgcggttctc cggaaacagg aactcgaggg tgccgcgcga
     2341 cagcgcccgc gagcgatgcc gcagcgcgat gacgcgcttc atccaccaca gcagcgagtg
     2401 ggggttggcc tgctgcgcct cgacgttgat cgcctcgtag tggtactcgg ggtcgatgat
     2461 caccggcagg aagaggcgct gggggttggc gcggctgaag cccgcgttgc ggtcgccgga
     2521 ccattgcatg ggggtgcgca cgccgttgcg gtcgcccagg tagatgttgt cacccatccc
     2581 gatctcatcg ccgtagtaga cgaccggcgt gccgggcatc gagaagagca gcgctttcat
     2641 cagctccatc gtccggcggt cgttcccgat caggggcgcc aggcgccggc ggatcccgag
     2701 gttgatccgc gcctgcgggt cggtcgcgta ggcccggtac atgtagtcgc gctcctcgtc
     2761 ggtgaccatc tcgagcgtca gctcgtcgtg gttgcgcaag aagactgccc actgcgcgcc
     2821 gtcgggaatc gacggcgtct gctgcaggat gtcgatgatc gggaagcggt cctccatccg
     2881 tgacgccatg aagagtc
//
LOCUS       DASPWO010000587         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_252622, whole genome shotgun sequence.
ACCESSION   DASPWO010000587 DASPWO010000000
VERSION     DASPWO010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252622"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VEO00_13390"
     CDS             <1..208
                     /locus_tag="VEO00_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59023.1"
                     /translation="ASTHYTPVLFDLGPPTADNAQCGFVPAQDACTMTRGFLNARCRN
                     GLASGKDLVPGQQYRAGVRFIDND"
     gene            208..381
                     /locus_tag="VEO00_13395"
     CDS             208..381
                     /locus_tag="VEO00_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59024.1"
                     /translation="MLKAGHRVGIALMSSNLWPALPDQNRSTNTPLSVRARRSTLVLP
                     VVGGKAAAVAAGL"
     gene            622..1701
                     /locus_tag="VEO00_13400"
     CDS             622..1701
                     /locus_tag="VEO00_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYU59025.1"
                     /translation="MTTVAMAFEELGLSEEIVHFLERDPGLRVVGTAADPAALSRLVR
                     DERPAVVVGSPSMLAGPAVNGEVRFAVAERETVASLRDAIRSGVSGFYLWPEERAHLA
                     GDAIRLSRRREELAGPRGRVVAVYGPRGGAGATFLATHLAAACASRGARTCLVDCDAF
                     FADVTSALGVAPKGSTRTIADLLPLLEELSAEHLDEVLYEHPRGFRVLLAPNDPASAD
                     AVQPAHLAAAVGVLTGEFDLILLHLPRSMDDVARSAVEMADEVLLVVTLDVLAFRDAK
                     RALTFLGGLGLDRKCRLVINRAARSEVVPEDVERVFGMRPAAVLAVDRAVTTAQNRGE
                     LLIGRSGRTARRIAALAGSLVEPAS"
     gene            1722..>2006
                     /locus_tag="VEO00_13405"
     CDS             1722..>2006
                     /locus_tag="VEO00_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HYU59026.1"
                     /translation="MTAPPAPAAASPDLPALTGAIRRRLVEGMDLAALEEMPGPERRL
                     HVRERVLAILRDERALLPAGELNALVNRISDDVVGLGPVERLLKDPEVSEV"
BASE COUNT          231 a          737 c          752 g          286 t
ORIGIN      
        1 ggcctccacg cactacacgc cggtcctgtt cgacctcggc ccgccgacgg ccgacaacgc
       61 gcagtgcggg ttcgtgccgg cgcaggacgc gtgcaccatg acccgcgggt tcctgaacgc
      121 gcggtgccgc aacggcctgg cgagcggcaa ggacctcgtc ccgggacagc agtaccgcgc
      181 cggcgtgcgg ttcatcgaca acgactgatg ttgaaggccg gccaccgcgt cggcatcgcg
      241 ctgatgagct cgaacctgtg gccggcgctg cccgaccaga accggtcgac caacacgccg
      301 ctatcggtac gggcgaggcg ctccaccctc gtcctgcccg tcgtcggcgg gaaggccgcc
      361 gccgtggccg ccggcctgta gcggcgggac gcacccggag gccccccgcc cgggtggggt
      421 cgaatccccc ggcgggggcc gtgaatcccc cggcctgggg atggtgcggt gccgccccgt
      481 cctgtaccgt tcgccccggc tcgccgcgaa cgcccccggc gatccttccc ccggcacctc
      541 tccctcccac ttctccacgg cgccttcccc gttccttccc cggttggctt cggcagggca
      601 ggaggtccga tgggacggtc ggtgacaacg gtcgcgatgg cgttcgagga gctcgggctc
      661 tccgaggaga tcgtgcactt cctcgagcgt gaccccgggc tgcgggtggt cggcaccgcg
      721 gccgaccccg cggcgctctc ccggctggtg cgcgacgagc ggccggccgt ggtggtcggg
      781 tccccgtcga tgctcgcggg cccggcggtg aacggcgagg tgcgcttcgc ggtcgcggag
      841 cgggagacgg tggcatcgct gcgcgacgcg atccgctcgg gcgtctccgg attctacctc
      901 tggcccgagg agcgggccca cctggcgggc gacgcgatcc ggctgtcgcg gcgcagggag
      961 gagctggccg gaccgcgcgg acgggtggtt gcggtctacg gaccgcgcgg cggcgccggg
     1021 gcgacgttcc tggccacgca cctcgccgcc gcctgcgcgt cacgcggcgc gcggacctgt
     1081 ttggtggact gcgacgcctt cttcgccgac gtgacctcgg cgctcggcgt ggctccgaag
     1141 ggaagcacgc ggacgatcgc cgacctgttg ccgctcctcg aggagctgtc ggccgagcac
     1201 ctcgacgagg tgctgtacga acacccgcgc ggcttccggg ttttgctcgc ccccaacgac
     1261 ccggcgagcg ccgacgcggt ccagccggca caccttgcgg ccgcggtcgg cgtcctgacc
     1321 ggcgagttcg acctgatcct gctccacctt ccccggagca tggacgacgt cgcacgctcg
     1381 gccgtggaga tggccgacga ggtgctgctc gtcgtcacgc tcgacgtcct ggcattccgc
     1441 gacgcgaagc gagccctgac gttcctcggc gggctcgggc tggaccggaa gtgccgcctg
     1501 gtgatcaatc gcgccgcgcg ctccgaggtg gtgccggagg acgtggagcg ggtgttcggg
     1561 atgcgtccgg cggcggtgct ggccgttgat cgcgcggtga cgaccgcgca gaaccgcggg
     1621 gagctgctga tcgggcggtc cggccgcacg gcccggcgga tcgcggcgct cgccgggtcg
     1681 ctcgtggagc cggcctcgtg agcgcccgcc aatcctggcc cgtgaccgct ccgcccgcgc
     1741 cggccgcggc gagccccgac ctgcccgcgc tgaccggcgc gatccgacgg cggctggtgg
     1801 aggggatgga tctcgccgcg ctcgaggaga tgccgggccc cgagcggcgg ctgcacgtcc
     1861 gggagcgcgt cctggcgatc ctgcgcgacg agcgcgcgct cctgccggcc ggcgagttga
     1921 acgcgctggt caaccggatc tcggatgacg tcgtcggcct cggtcccgtc gagcgcctgc
     1981 tcaaggaccc ggaggtgagc gaggtg
//
LOCUS       DASPWO010000588         3834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_931519, whole genome shotgun sequence.
ACCESSION   DASPWO010000588 DASPWO010000000
VERSION     DASPWO010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931519"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VEO00_13410"
     CDS             <1..475
                     /locus_tag="VEO00_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYU59027.1"
                     /translation="GKTLLAPGMRVGYVALPPTMTDREQVRGALGLSIIALGWAYPNA
                     LVQHALPEFEDLSIDLAHLERKRDRMVAGLREAGYTLHVPEGAFYLLPASPWRDDEAF
                     TDLLASKDVFVLPGRMVEWPGHFRISITANDDMIDRALPRFAEALAEARAAPVPS"
     gene            560..994
                     /locus_tag="VEO00_13415"
     CDS             560..994
                     /locus_tag="VEO00_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYU59028.1"
                     /translation="MNVGSVMAGAVETVAPDTSVEDVARIMAKAEVGSVPVTEDGLLA
                     GIVTDRDLVVRVLAEGRDPRSVTAGEVASTDVVTVGPDRPLAEARELMARNQIRRLPV
                     VDEGRLVGILALGDIAVQDDAEQATGEALEEISRSPATESRG"
     gene            complement(1021..1779)
                     /locus_tag="VEO00_13420"
     CDS             complement(1021..1779)
                     /locus_tag="VEO00_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYU59029.1"
                     /translation="MIRVAATGDLHFGQDSAGTLRPHLDGLARDADLLLIAGDLTKAG
                     EPDEAAVLAAELRDLPVPAVAILGNHDHHADREDEVAAAVEDAGVAVLRERGTVVHVA
                     GLRVGIAGAKGFGGGFPGASASDFGEPEMKAFVRHTKNIAVLFEAALAELEADLRIAL
                     LHYAPVRETLEGEPLEIYPFLGSSLLGEAIDRAGADLALHGHAHRGSEHGRTPGGVEV
                     RNVALSVIDAAYRVYRLDGSMNRRLTSGAAATPG"
     gene            complement(1776..2036)
                     /locus_tag="VEO00_13425"
     CDS             complement(1776..2036)
                     /locus_tag="VEO00_13425"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYU59030.1"
                     /translation="MTTGGEHDAYLLERIRDRLAADERVHELELELQVSAGRIVVRGR
                     VPTPERRRAIGEVIAEVAPDHDVSNETELVDLSVDGRQEEVT"
     gene            complement(2033..2659)
                     /locus_tag="VEO00_13430"
     CDS             complement(2033..2659)
                     /locus_tag="VEO00_13430"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HYU59031.1"
                     /translation="MASNRSAVHEREDAALVEALRLAVGALEGAEIPHLVFGGIAAVL
                     YGRSHAVKDADVLVRGADADRALAVLERAGFETEVDNPNWLYKARRDEQIVDVIFQVK
                     GGIHLDDEMLARGRRAEFEGVPITLVSPEDALLMESVSFGAETPRHWFNALAILSQQR
                     LDWDIVQDRARLAPRRMLSLLVFAQGDLAVPDEVVKGLYQLVYEPEGP"
     gene            complement(2888..3316)
                     /locus_tag="VEO00_13435"
     CDS             complement(2888..3316)
                     /locus_tag="VEO00_13435"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYU59032.1"
                     /translation="MQAGLPSEGSLAGEVRAGSVCRADVPTCRLGDPLEDVRERAGAA
                     GWDVTVVTTEGGVVLGLLRPGDLAGARGRAEDAMLEAPSTFRANVPIVELAGHMVEHD
                     FELAPITRPEGTLIGLLRKDDAVAAALKVHQAEHDPDDDA"
     gene            complement(3401..3607)
                     /locus_tag="VEO00_13440"
     CDS             complement(3401..3607)
                     /locus_tag="VEO00_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYU59033.1"
                     /translation="MPRSVYERDAVRALVERGAQLVEVLPHEEYEEEHLPGAISLPLR
                     QIPDEARERLDPGRPVVVYCFDFP"
BASE COUNT          509 a         1429 c         1370 g          526 t
ORIGIN      
        1 cggcaagacg ctgcttgcgc cggggatgcg cgtcggctac gtcgcgctcc cgccgacgat
       61 gaccgaccgg gagcaggtcc ggggcgcgct cggcctctcg atcatcgcgc tcggatgggc
      121 gtacccgaac gcactggtac agcacgctct cccggagttc gaggacctgt cgatcgatct
      181 cgcgcacctc gagcgcaagc gcgaccgcat ggtcgccggc ctgcgcgagg cgggctacac
      241 gctgcacgtc cctgagggtg ccttctacct gctcccggcc tcgccctggc gggacgacga
      301 ggcgttcacc gacctgctgg cctcgaagga cgtcttcgtc ctgcccgggc ggatggtgga
      361 gtggccgggg cacttccgca tctcgatcac cgcgaacgac gacatgatcg accgcgccct
      421 gccgcgcttc gccgaggcgc tggccgaggc ccgggcggcc ccggttccct cctgagtgcc
      481 gtctcgcgcc ccgattgggc caccgccact gcgggtaggc ctgtcagcag gcggccaccg
      541 aacgaaggag gggatcgtcg tgaacgttgg cagcgtgatg gcgggcgccg tcgagacggt
      601 cgcgccagac acctcagtgg aagacgtggc gcggatcatg gccaaggccg aggtcggctc
      661 ggtcccggtc accgaggatg gcctgctcgc cggtatcgtc accgaccgtg acctggtggt
      721 ccgcgtgctg gccgaaggcc gggacccccg gtcggtcacc gcgggcgagg tggcctcgac
      781 cgacgtggtc acggtgggac cggaccggcc gctcgcggag gcgcgcgagc tgatggcccg
      841 gaaccagatc aggcgcctgc cggtcgtcga cgaggggaga ttggtcggga tcctcgccct
      901 cggcgacatc gccgtccagg acgacgccga gcaggccacc ggcgaggcgc tcgaggagat
      961 ctcccggtca ccggccaccg agtcacgcgg ctagcggacc gccgcagcgg ctcgccgagg
     1021 ctatccgggc gtggcggcgg ccccggaggt caatcgccgg ttcatgctgc cgtcgagtcg
     1081 gtacacgcga taggccgcgt cgatcaccga gagcgcgaca ttgcgaacct cgacgccgcc
     1141 gggcgtccgg ccgtgctcgc tcccgcggtg ggcgtgcccg tgcagcgcga ggtcggcgcc
     1201 ggcccggtcg atcgcctcgc ccagcaggga gctcccgagg aacgggtaga tctcgagcgg
     1261 ttcgccttcc agcgtctcgc ggacgggcgc gtaatggagc agggcgatcc gcaggtcggc
     1321 ctccagctcg gcgagggccg cctcgaagag cacggcgatg tttttggtgt gcctgacgaa
     1381 ggccttcatc tccggttcgc cgaagtcgct ggcgctcgcg ccggggaatc cgccgccgaa
     1441 ccccttggcc ccggcgatcc cgacgcggag ccccgccacg tggaccaccg tgccccgctc
     1501 gcgcagcacg gccacgccgg cgtcctcgac cgccgccgcc acctcgtcct cgcggtcggc
     1561 gtggtggtcg tgattgccga ggatcgccac cgccgggacc ggcaggtccc gcagctctgc
     1621 cgccagcacg gccgcctcgt ccggctcgcc ggccttggtc aggtcgccgg cgatgagcag
     1681 caggtcggcg tcgcgagcga ggccgtccag gtgggggcgc agtgtcccgg ccgagtcctg
     1741 cccgaaatgc aggtctccgg tggccgccac ccggatcacg tgacctcctc ctgccggccg
     1801 tcgaccgaga gatcgaccag ctcggtctcg ttgctgacgt cgtgatcggg ggcgacctcg
     1861 gcgatgacct cgccgatcgc ccggcgccgc tccggcgtcg ggacgcggcc tcggaccacg
     1921 atccggccgg ccgacacctg cagctcgagc tccagctcgt gcacgcgctc gtcggcggcc
     1981 agccggtcgc ggatccgctc cagcaggtag gcgtcgtgct cgcccccggt cgtcacggcc
     2041 cctccggttc gtagaccagt tggtacagtc ccttcaccac ttcgtcgggc accgcgaggt
     2101 cgccctgggc gaagaccagc aggctgagca tccgacgggg cgcgagtcgg gcgcggtcct
     2161 gcacgatgtc ccagtcgagc cgctgctggg agaggatcgc cagcgcgttg aaccagtgcc
     2221 gcggcgtttc ggcgccgaag ctcaccgact ccatcaacag agcgtcctcc ggcgagacga
     2281 gggtgatcgg gacgccctcg aactcggccc gacggccgcg cgcgagcatc tcgtcgtcga
     2341 ggtggatgcc gcccttcacc tggaagatca cgtcgacgat ctgctcatcg cgacgggcct
     2401 tgtagagcca attggggttg tcgacttccg tctcgaaccc ggcccgctcc agtacggcca
     2461 gggcgcggtc ggcgtcagcg ccgcgaacca gcacgtcggc gtccttcacc gcgtgcgatc
     2521 gcccgtagag caccgcggcg atgcccccga acaccaggtg ggggatctcc gccccctcga
     2581 gggcccccac cgcgaggcgc agcgcttcca cgagcgcagc gtcctcgcgc tcgtgcacgg
     2641 cgcttcggtt cgaggccatc ccggttcggc gatacccgct gccgtgtcag cgtaaccgcg
     2701 ctcagccccg tggccgtgcg gacggagcag ggactgcccg atggcctcta ccgtgcatcg
     2761 gtagcagcga gcggtcgggc aggcctccgc aacgcagagc gagcaggcgt cgagcgcccg
     2821 ctcgcacgtg acgcagacgg tcgccgcccc ccgccggggc tcccgcgccg gccgccgcgc
     2881 cgggggctca cgcatcgtcg tcggggtcgt gctcggcctg atgcaccttc agcgccgcgg
     2941 cgacggcgtc gtcctttcgc agcaggccga tcagcgtccc ctcgggcctc gttatcggcg
     3001 ccagctcgaa gtcgtgctcg accatgtggc ctgccagctc gacgatcggg acgttcgccc
     3061 ggaacgtgct cggggcctcg agcatcgcgt cctccgcgcg cccccgggcg ccggcgagat
     3121 cgcccggacg gagcaagccg agcaccacgc ccccctcggt cgtgacgacg gtgacgtccc
     3181 agccggccgc gcccgcccgc tcgcggacgt cctcgagcgg gtcgccgagc cggcaggtcg
     3241 gcacgtctgc ccggcagacc gatccggcgc gcacctcccc ggccagcgac ccctccgagg
     3301 ggaggccggc ctgcacccag tcctgcttcc cggggacgta gtcgtgaacg tccgcgaacc
     3361 cgagcgattc gagccggcac gcggcccgtg ggctgaggtc tcaggggaag tcgaagcagt
     3421 agacgaccac gggacggccc gggtcgagcc gctcccgcgc ctcgtcgggg atctgccgca
     3481 gcggcaggct gatcgcgccc ggcaggtgct cctcctcgta ctcctcgtgc ggcagcacct
     3541 cgaccagctg cgccccgcgc tcgacgaggg cccgtaccgc gtcccgctcg tagacgctcc
     3601 tcggcaccgt cacccccagg ccggacgagg ctcggactgc agccaccctt acccgggatc
     3661 gcggagcgcc atgcgaggac ctcgggttcg ccgcgccgga cgccgggtag gggaggggcg
     3721 tcgcagacgc ggatcaacgg agccgtgcgg acgagcaggg gcggtcaggc ctgtccgggc
     3781 gtgcggggga cgagcgccga gccgccggct gtgcgcagcg gcgcgtcgcg cgcg
//
LOCUS       DASPWO010000589         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_252953, whole genome shotgun sequence.
ACCESSION   DASPWO010000589 DASPWO010000000
VERSION     DASPWO010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252953"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2014
                     /locus_tag="VEO00_13445"
     CDS             <1..>2014
                     /locus_tag="VEO00_13445"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYU59034.1"
                     /translation="GGTGGILFISGEAGIGKSRLLAELRDRFESGPAQTPQPPLWLEG
                     RCVSYGDSLPYWPYRDLLREWLGAGVDDPELRVRVALRRRIEALFGARALEVYPYLGA
                     MLGLALEPDASSRLSELSPEALQYRTFEIVGALFTRLAEDGPVVVALDDLHWADPTSV
                     QLTERLLPLAEDAAVLFVLSQRTERDHPSWMVLESALRDLPHRTRQIALEALSGDADR
                     ELLHALVGAGTLPPDTERRLLAVAEGNPFFLEELVRTLVDAGALVVEADGRRFDQEVA
                     TPIPPTVEKVVLARIDRLTPACRSVLTAASVLGRRFGLSLLEGVCGADDPVRESLRDL
                     MRLDLVREGRRWPQPEYRFKHALIQEAAYRTLLAEHRTRLHRRAAEWLERQYAENPDE
                     VFGLLAHHWLAAADEDKAIDYLTRAGDKARLEYALDEAVEHYRELLPLLERRGERRAI
                     ALVLFKLALALHTSLRFAEANAAYQRAFAFWDPPEEATSAPAATFRIASSFVPNDPDP
                     RSAIAWPNIQLCMQLFDPLVEAWPERTIVPALAERWEISDDGLRYVFHLRPGLRWSDG
                     KQLTAHDVEFGIKRVLDPVRPGSSVAIYFVLENGQDYAMGRSPDAARVGVRALDDLTV
                     EFRLAAPAPYFMSVMNRPDGGPQPRHAIERDGDAWVETGRQVVSGP"
BASE COUNT          250 a          753 c          715 g          296 t
ORIGIN      
        1 tcggtgggac cggagggatc ctgttcatct cgggcgaggc cgggatcggc aagagccggc
       61 tcctggccga gcttcgggac cgattcgagt ccggcccggc ccagactccc cagccgcccc
      121 tctggctgga aggccggtgc gtctcctacg gcgactcgct gccctactgg ccctaccgcg
      181 acctgctgcg cgaatggctc ggcgccggcg tggacgatcc cgagctccgc gtgcgggtgg
      241 cgctccggcg gcgcatcgag gcactcttcg gtgcccgcgc cctggaggtc tacccctacc
      301 tcggggcgat gctcggcctg gcgctcgagc cggacgcgtc gtcgcgcctg tccgagctct
      361 ccccggaggc cctgcagtac cgcaccttcg agatcgtcgg ggccctcttc acccgcctgg
      421 ccgaggacgg gccggtggtc gtcgccctgg acgacctgca ctgggccgac ccgacctcgg
      481 tgcagctgac ggagcggctg ctcccgctcg ccgaggacgc cgccgtcctg ttcgtcctca
      541 gccagcgtac cgagcgggac cacccgtcct ggatggtgct cgagtccgcc ctgcgcgatc
      601 ttccccaccg cacgcgccag atcgccctgg aggccctctc cggcgacgcg gaccgcgagc
      661 tgctccatgc cctggtcggc gcggggaccc tgccgcccga cacggagcgc cggctcctcg
      721 cggtggccga gggcaacccg ttcttcctgg aggaactggt caggacgctg gtcgacgccg
      781 gggccctggt ggtggaggcg gacggtcgcc gcttcgacca ggaggtggcc acgccgatcc
      841 cgccgacggt ggagaaggtc gtgctcgctc gcatcgaccg gctgaccccc gcctgccgtt
      901 cggtgctcac cgccgcctcg gtgctcggac ggcggttcgg gctgtccctg ctggagggcg
      961 tgtgcggggc ggacgacccg gtgcgcgagt cgctgcggga cctgatgcgc ctggacctgg
     1021 tccgcgaggg acgccgctgg ccgcagcccg agtaccgctt caagcacgcg ctgatccagg
     1081 aggccgccta ccggacgctc cttgccgagc atcggacccg gctgcaccgc cgggccgccg
     1141 agtggctgga gcgccagtac gccgagaacc cggatgaggt cttcggcctc ctggcccacc
     1201 actggctggc ggccgccgac gaggacaagg cgatcgacta cctgacccgg gcaggcgaca
     1261 aggctcggct ggagtacgcg ctcgacgagg cggtcgagca ctaccgcgag ctgctgccgc
     1321 tcctcgagcg ccgcggcgag cgacgggcga tcgcgctggt gctgttcaag ctggcgctcg
     1381 cgctgcacac gtcgctgcgc ttcgccgagg cgaacgccgc gtaccagagg gcgttcgcgt
     1441 tctgggatcc cccggaggag gcgacctcgg cgccggcggc gacgttccgc atcgcgtcga
     1501 gcttcgttcc gaacgacccg gacccgcgct ccgcgatcgc ctggccgaac atccagctct
     1561 gcatgcagct cttcgacccg ctggtcgagg cctggccgga acggacgatc gtccccgccc
     1621 tcgccgagcg ctgggagatc tccgacgacg gcctgcgcta cgtcttccac ctccgtccgg
     1681 ggttgcgctg gtccgacggg aagcagctca ccgcccacga cgtcgagttc gggatcaagc
     1741 gggtgctcga tccggtccgg cccggttcct cggtggcgat ctacttcgtg ctcgagaacg
     1801 gccaggacta cgcgatgggg cgcagccccg acgccgcgcg cgtcggcgtg cgcgcgctgg
     1861 acgacctcac ggtcgagttc cggctggccg cgccggcccc ctacttcatg agcgtgatga
     1921 accggccgga cggcggcccg cagccccgcc acgccatcga gcgcgacggc gacgcctggg
     1981 tcgagacggg ccggcaggtg gtcagcggtc ccta
//
LOCUS       DASPWO010000590         4851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1102795, whole genome shotgun sequence.
ACCESSION   DASPWO010000590 DASPWO010000000
VERSION     DASPWO010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102795"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VEO00_13450"
     CDS             complement(<1..875)
                     /locus_tag="VEO00_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59035.1"
                     /translation="MKRAALVVGLVLLAGGGGLFGYGMLAGGGDGDGDEPASFAFTNG
                     TLCEACTLNITTAEVQDRDGSTLFVFGGDWPDTPGEVPGDLRLAANDLELVLHPVDAG
                     FEVPTANAGGQAVDPAEFAVGLQGGAFLLDVRDPLLAAPVDFVFGLWDGTDYGARIPA
                     QGSLHWDGSGAPAPFVPPTSPTPTATETTVAPEEETAQQFTADLARAIQTGDEAFLFE
                     RLGQATIARYGEEACRTYAAGIHDPTRAYTVTDVGEPAPWDYVTDGLTTTVPDVVAVA
                     VTGTAEGQPFKGHLH"
     gene            complement(872..2104)
                     /locus_tag="VEO00_13455"
     CDS             complement(872..2104)
                     /locus_tag="VEO00_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59036.1"
                     /translation="MKQDGAGGGGGGGAAKPPRPEVPKEAPADLRDFVDCKGRTVELK
                     PGEIGWFPFPEDVKKYAPTPKASFEHGDKPGTVNVTISLGLSMTLPVSVQNGDLSIDT
                     SGIMIPGGAGKTTNWVNQFNGWLKANGWKLDPLKLRRGVVTLTKVAVVEAPKQAVAPG
                     EPKKEEPKKEEPPKGGGKIKAAAATGAFILGTGTGALFGLNAGPDGDVGLGQPSDPVI
                     TFESAPPSASITITVLPEGGVDANLIVLPASGGGSFPLSGHFGQDGSALTFQVPDLPP
                     FVGTMGGDGTFDVRNDVGSFTGLFSGNQVEGDHVFQGEAFTFSGSLSAAVQTIVEPGE
                     APYTYKIKDDLRFHSGEPVSGGDTGNALEGTTGIVTGLGTAAVGTGLVAASGRRRRRR
                     RRRRRRRAAEPPPEDRGP"
     gene            complement(2231..2842)
                     /locus_tag="VEO00_13460"
     CDS             complement(2231..2842)
                     /locus_tag="VEO00_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59037.1"
                     /translation="MRRSLVMAAAIVAVVALLAGQALAADIIGTNGSDTIQGTPGNDN
                     INGLNGHDTIYGNNGGDIILGSYGFDKLIGNDDPDTLYGNAGNDRLWGEVQLCGYTPS
                     PARDDKGGGPTFTPCPGPDLDKFYGGNNNDVIHADDMPLFTTNQADPTIIGDPSQGEF
                     VSCGAGSGDVAWVDNNDYARAQVNASPLFQTCETIVMNGQVQP"
     gene            3007..4347
                     /locus_tag="VEO00_13465"
     CDS             3007..4347
                     /locus_tag="VEO00_13465"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HYU59038.1"
                     /translation="MEAITSLAKRRGLIFPSSEIYGGLRSSWDYGPLGVELKRNVKDA
                     WWRAMVQLRDDIVGLDAAIIMKPEVWEASGHVESFADPLVECRDCHRRWRLDHLPDWK
                     PGTPVEELRCPDDGGRLSDPRQFNLMFRTFLGPVEDEAHLAYLRPETAQGIFVDFRTV
                     QAASRRKVPFGIAQIGKSFRNEITPGNFIYRTREFEQMEMEFFVEPGTDETWHEYWIE
                     ERLRWYRDLGIRDEHLRIREHAADELSHYSKRTVDIEYAFPFTDWGELEGIANRTDFD
                     LKAHEKLSGQDLTYYDQERDVRYHPYVIEPAAGADRATLAFLVDAYSVDEAPTAKGTM
                     EKRTVLRLHRDLAPTKVAVLPLSRHEDLVPEAKRVFDVVKPHWMTDYDDAGAIGRRYR
                     RQDEVGTPYCVTVDFDTLQDRAVTVRDRDSMAQERIEIVKLLDHLRERLDGAPA"
     gene            4442..>4851
                     /locus_tag="VEO00_13470"
     CDS             4442..>4851
                     /locus_tag="VEO00_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59039.1"
                     /translation="MTPRGRAAPWAIGGAIVAVLSIGVVVGANLAQDEDALPTPGPRR
                     SPGVEVSSDRARATLCRRPPLAEPEPQDQGSLPAAVATIVQQAEQVRELRFERPVRPE
                     AISRERMGQEIARALERSYPREMMDRRTQAWRTIG"
BASE COUNT          671 a         1741 c         1727 g          712 t
ORIGIN      
        1 aagtgaagat gtcccttgaa cggctggccc tcggccgtcc cggtgacggc cacggccacc
       61 acgtcgggaa ccgtggtggt cagcccgtcg gtgacgtagt cccagggggc cggctcgccg
      121 acgtcggtca ccgtgtaggc ccgggtaggg tcgtggatcc ccgcggcgta cgtgcgacag
      181 gcctcctccc cgtagcgggc gatcgtcgcc tgccccagcc gctcgaacag gaacgcctcg
      241 tccccggtct ggatggcgcg agcgaggtcg gcggtgaact gctgcgcggt ctcctcctcc
      301 ggcgccaccg tggtctccgt ggcggtcggg gtgggcgacg ttggcgggac gaagggcgcc
      361 ggcgcgcccg acccatccca gtgcaacgaa ccctgggcgg ggatccgcgc gccgtagtcc
      421 gtcccgtccc acaggccgaa cacgaagtcg accggggccg ctagcagcgg gtcgcggacg
      481 tcgagcagga atgcgccgcc ctggagcccg accgcgaact ccgccgggtc cacggcctgg
      541 ccgccggcgt tcgccgtcgg cacctcgaac ccggcgtcga ccgggtgcag caccagctcc
      601 aggtcgttgg ccgcgagccg gaggtcgccg gggacctcgc ccggcgtgtc cggccagtcg
      661 ccgccgaaga cgaacagcgt gctgccgtcc cggtcctgca cctcggcggt ggtgatgttc
      721 agcgtgcacg cctcgcagag cgtgccgttc gtgaacgcga aggatgccgg ctcgtcgccg
      781 tcgccgtcgc cgccgcccgc gagcatgccg tacccgaaca gcccgccgcc gcccgcgagc
      841 aggacgagcc cgacgaccag ggcggcccgc ttcacggccc tcggtcctcc ggcggcggct
      901 cggccgcgcg ccgcctgcgc cgcctgcgcc gcctgcgccg ccgccggccg ctcgcggcga
      961 ccagtccggt cccgaccgcg gcggtgccca ggccggtgac gatccccgtc gtgccctcga
     1021 gcgcgttccc cgtgtcgccg cccgagaccg gctccccgga atggaacctg aggtcgtcct
     1081 tgatcttgta ggtgtacggc gcctcaccgg gctcgacgat cgtctggacc gccgccgaca
     1141 gggaccccga gaacgtgaac gcctcaccct ggaagacgtg gtcgccctcc acctggttcc
     1201 cggagaacag gccggtgaag ctcccgacgt cgttgcgcac gtcgaacgtg ccgtcgccgc
     1261 ccatcgtccc gacgaacggc gggaggtcgg ggacctggaa cgtcagggcg ctcccgtcct
     1321 gcccgaagtg gccgctcagc gggaacgacc cgccgccgga cgccggcagc acgatcaggt
     1381 tggcgtccac gccgccctcc gggagcaccg tgatggtgat ggaggcggac ggcggggccg
     1441 actcgaacgt gatcactgga tctgacggct gccccaggcc gacgtcgccg tccggccccg
     1501 cgttcaggcc gaacagcgcc ccggtgccgg tcccgaggat gaaggcgccg gtcgccgcgg
     1561 cggccttgat cttgccgccg cccttcggcg gctcctcctt cttcggctcc tccttcttcg
     1621 gctcccccgg cgcgaccgcc tgcttgggcg cctcgaccac cgcgaccttc gtcagggtca
     1681 ccacgccgcg gcggagcttc agggggtcga gcttccagcc gttcgccttc agccagccgt
     1741 tgaactggtt cacccagttc gtcgtcttcc cggcgccgcc ggggatcatg atcccggacg
     1801 tgtcgatcga caggtcgccg ttctgcaccg agacgggaag ggtcatcgag agcccgagcg
     1861 agatcgtcac gttgacggtc ccgggcttgt ccccgtgctc gaaggacgcc ttcggcgtgg
     1921 gggcgtactt cttgacgtcc tcggggaacg ggaaccatcc gatctccccg ggcttcagct
     1981 cgacggtcct gcccttgcag tcgacgaagt cgcggaggtc ggcgggggcc tccttcggga
     2041 cctcgggacg gggaggcttc gcggcgccac cgccgccgcc gcctcccgca ccgtcctgct
     2101 tcacgacgcc tcctgccccg gccggctcgc ggtcagaaga cggtcccgtt gatgaaggcc
     2161 accacgaaga gggatctgcg catcgccgtt ccgtgcgctc tccgggtcgc gccgccggtg
     2221 ggcggaccgg tcacggctgg acctgcccgt tcatcacgat cgtctcgcag gtctggaaca
     2281 gcgggctcgc gttcacctgg gcccgggcgt agtcattgtt gtcgacccag gccacgtcgc
     2341 cgctcccggc gccgcagctc acgaactcgc cctgcgacgg atctccgatg atcgtcggat
     2401 cggcctggtt cgtcgtgaag agcggcatgt cgtcggcgtg gatcacgtcg ttgttgttgc
     2461 cgccgtagaa cttgtccagg tccgggccgg ggcacggcgt gaacgtcggg ccgccgccct
     2521 tgtcgtcgcg cgcgggcgag ggagtgtagc cgcagagctg cacctcgccc cagagcctgt
     2581 cgttcccggc gttgccgtac agcgtgtcgg ggtcgtcgtt tccgatcagc ttgtcgaagc
     2641 cgtaggaccc gaggatgatg tcgcccccgt tgttcccgta gatcgtgtcg tgcccgttca
     2701 acccgttgat gttgtcgttg ccgggcgtgc cctggatcgt gtcgctgccg ttcgtgccga
     2761 tgatgtcggc ggccagggcc tggcccgcca gcagcgcgac cacggccacg atcgcggccg
     2821 ccatgacgag ggatctgcgc atcgagtctc gctttcggaa cggcggccgg cggccgcggt
     2881 gcgcgtagtg tgcccccgcg acccgctgcg cacaagaccc gaatccgatc ccggcccgta
     2941 tcatggcccg acctccacgg cacccagtcg cgggagaaag gagacggatc cgacatgccc
     3001 gacctgatgg aggccatcac ctccctcgcc aagcggcgcg gcctgatctt cccgtcgagc
     3061 gagatctacg gcgggctgcg ctcgtcctgg gactacggtc cgctcggggt cgagctgaag
     3121 cgcaacgtca aggacgcctg gtggcgggcc atggtccagc tccgcgacga catcgtcggg
     3181 ctcgacgccg cgatcatcat gaagcccgag gtctgggagg cgagcgggca cgtcgagtcg
     3241 ttcgccgacc cgctggtgga gtgccgcgac tgccatcggc gctggcgcct ggaccacctg
     3301 ccggactgga agccgggcac gccggtcgag gagctgcgct gtccggacga cggcgggcgg
     3361 ctctccgacc cgcggcagtt caacctgatg ttccggacgt tcctcgggcc ggtcgaggac
     3421 gaggcgcacc tggcctacct ccgtcccgag accgcgcagg gcatcttcgt ggacttccgc
     3481 acggtgcagg cggcgagccg gaggaaggtc ccgttcggga tcgcccagat cgggaagtcg
     3541 ttccgcaacg agatcacgcc ggggaacttc atctaccgca cgcgcgagtt cgagcagatg
     3601 gagatggagt tcttcgtcga gccgggcacc gacgagacct ggcacgagta ctggatcgag
     3661 gagcggctgc gctggtaccg ggacctcggg atccgcgacg agcacctccg gatccgcgag
     3721 cacgccgccg acgagctgtc gcactactcg aagcgcacgg tggacatcga gtacgcgttc
     3781 ccgttcaccg actgggggga gctcgagggg atcgccaatc gcaccgactt cgacctgaag
     3841 gcgcacgaga agctctccgg ccaggacctg acctactacg accaggagag ggacgtccgc
     3901 taccacccgt acgtgatcga gccggcggcc ggggccgacc gcgccacgct cgcgttcctg
     3961 gtggacgcct acagcgtgga cgaggccccg accgccaagg ggacgatgga gaagcgcacg
     4021 gtgctgaggc tgcaccggga cctggctccg acgaaggtgg ccgtgctccc gctgtcgcgc
     4081 cacgaggacc tggtgcccga ggcgaagcgc gtcttcgacg tggtcaagcc gcactggatg
     4141 accgactacg acgacgccgg cgcgatcggg cgtcgctacc ggcgccagga cgaggtcggg
     4201 accccctact gcgtgacggt cgacttcgac acgctgcagg accgcgcggt caccgtgcgc
     4261 gaccgggaca gcatggcgca ggagcggatc gagatcgtaa agctccttga ccatctgcgc
     4321 gagcggctgg acggcgcgcc ggcgtgacgc cggatcccga ggtcgaggcg cgcgccctga
     4381 cgatcgagga gaacgtcgcg tcgttcttgc cggcctgcgc gcgcgtggtc gagtgggcac
     4441 cgtgaccccg aggggccggg ccgctccctg ggcgatcggg ggggcgatcg tcgcggtgct
     4501 gtccatcggc gtcgtcgtgg gagcgaacct cgcccaggac gaggacgcgc tcccgacgcc
     4561 cggcccgcgc cgcagccccg gggtggaggt ctcgtcggac cgggcccggg ccacgctctg
     4621 ccgccgcccg cccctcgcgg agccggagcc gcaggaccag ggctcgctgc cggcggcggt
     4681 ggccacgatc gtgcagcagg cggagcaggt ccgggagctg cgcttcgagc gcccggtccg
     4741 gccggaggcg atcagccgcg agcggatggg acaggagatc gcccgcgcgc tggagcgctc
     4801 ctacccgcgg gagatgatgg accgccggac gcaggcctgg cggacgatcg g
//
LOCUS       DASPWO010000591         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_505799, whole genome shotgun sequence.
ACCESSION   DASPWO010000591 DASPWO010000000
VERSION     DASPWO010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505799"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VEO00_13475"
     CDS             complement(<1..475)
                     /locus_tag="VEO00_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HYU59040.1"
                     /translation="MGRTESRGGSSTFRRKRSPSSRRSGRGSSRSRSGGGDSPAIPTA
                     PTLRDVAKRHGVRPSKALGQHFLTDPNLARAIVADAGIGPGDRVLEVGAGLGALTVAL
                     AAAGAHVLAVELDRGMVPALQEVVEGLDVDVVRADATTADWRALLGTERWAMVSNL"
     gene            complement(363..716)
                     /locus_tag="VEO00_13480"
     CDS             complement(363..716)
                     /locus_tag="VEO00_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HYU59041.1"
                     /translation="MRRIATLLALAVLVLGPTGTSPAGARTVHAAAVRSDTGDASWYD
                     APGDELEAAHRTLPFGTKVLVKNRQNGNRVVVVINDRGPYGVPGRIIDLSSEAFAKLA
                     PLGQGVIQVKIWWRG"
     gene            complement(736..1545)
                     /locus_tag="VEO00_13485"
     CDS             complement(736..1545)
                     /locus_tag="VEO00_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HYU59042.1"
                     /translation="MRDAADPGALAGLPPIVDTHAHLFLMDAPPAAVVARAVEDGIAQ
                     LVCAAIDPETTDASIVIAEAHPEVFATSGMHPHTASTLDEEAKRAIADRAGRPNVVAI
                     GETGLDFFRMHSPRGDQLASFRWHVGLSNETGKPVIVHVRDAWEDVLRVLAEERAERV
                     VIHCFSGGVGEARECAARGYRLSFAANVTYPKNAGLRKAAAAAPRGSIVVETDSPFLP
                     PEGSRGKENVPGNARVALRALAEARGEDPVALAGAVAANARAVFGLPVDAA"
     gene            complement(1542..>2222)
                     /locus_tag="VEO00_13490"
     CDS             complement(1542..>2222)
                     /locus_tag="VEO00_13490"
                     /inference="COORDINATES: protein motif:HMM:NF039287.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYU59043.1"
                     /translation="MSKTKLTGIHPLELIDQFGTDAYRYYFLREVQFGADGSFSWESM
                     VARYNADLANDYGNLASRVLAMTAAYFDGMVPEPPAGPGPESRLAEVAAAAAGRYDER
                     MLALDLTEGIAAVWDFVKEVNRYLVEVAPWALAKDPARRDDVARAIYAGLESLRILAI
                     LTAPVMPGTSAKLWASLGIEAPLAEQVLPGAAAWGGLRPGTRTTRGEALFPRIEVDEG
                     AEAEGAGE"
BASE COUNT          299 a          846 c          783 g          294 t
ORIGIN      
        1 ggagattcga caccatcgcc caccgctcgg taccgagcag cgcgcgccag tcggccgtcg
       61 tcgcgtcggc gcggacgacg tcgacgtcga ggccctcgac cacctcctgc agcgccggca
      121 ccatcccgcg atcgagctcg accgccagga cgtgcgcgcc ggccgcggcc agcgccaccg
      181 tcagggcacc gaggccggcg ccgacctcga gcacacggtc acccggaccg atcccggcgt
      241 cggcgacgat cgcgcgagcc aggttcgggt cggtcaggaa gtgctggccg agggccttcg
      301 agggccggac gccgtgacgc ttcgcgacgt cgcgcagggt gggcgcggtg gggatggcgg
      361 ggctatcccc gccaccagat cttgacctgg atgaccccct gcccgagcgg cgcgagcttg
      421 gcgaacgctt ccgacgaaag gtcgatgatc cgccccggga ctccgtacgg cccacggtcg
      481 ttgattacca cgaccacgcg attgccgttc tgccggttct tcaccaggac cttcgtcccg
      541 aacggaagcg ttcggtgggc ggcctccagc tcgtcgcccg gcgcgtcgta ccaggacgcg
      601 tcgcccgtgt ccgacctcac cgcggccgcg tgcaccgtcc gcgcgcccgc cggcgacgtc
      661 ccggtcggac cgagcacgag cacggccaac gcgagaaggg tggcgatccg acgcatcgcc
      721 cgaccctacc ggagatcagg cggcgtcgac cggaagcccg aagaccgcgc gtgcgttcgc
      781 ggcgacggcc ccggcgagcg ccaccgggtc ctcgccccgt gcctcggcca gcgcgcgaag
      841 cgcaacgcgc gcgtttcccg gcacgttctc cttcccgcga ctgccctccg gcggcaggaa
      901 cgggctgtcg gtctccacca cgatgctgcc ccggggcgcc gcggccgccg ccttccggag
      961 gccggcattc ttcgggtagg tcacgttggc ggcgaacgac agccggtagc cccgcgcagc
     1021 gcactcgcgc gcttcgccga caccgcccga gaagcagtgg atcaccaccc gctcggcccg
     1081 ctcctcggcg agcacgcgca gcacgtcctc ccaggcgtcg cggacgtgca cgatgacggg
     1141 cttgccggtc tcgttcgaca gtccgacgtg ccagcggaac gacgccagct ggtcgccgcg
     1201 cggcgagtgc atccggaaga agtcgagtcc ggtctcgccg atggcgacca cgttcgggcg
     1261 gccggcccgg tcggcgatcg cgcgcttcgc ctcctcgtcg agcgtggagg ccgtgtgcgg
     1321 gtgcatgccg gaagtggcga agacctccgg gtgggcctcg gcgatgacga tcgacgcgtc
     1381 ggtggtctcg gggtcgatcg ccgcgcagac gagctgcgcg atcccgtcct cgaccgcgcg
     1441 ggcgaccacc gccgcgggcg gggcgtccat caggaacagg tgcgcgtgcg tgtcgacgat
     1501 cggcgggagc ccggcgagcg ctcccgggtc cgccgcgtcc ctcactcgcc ggcgccctcc
     1561 gcttccgctc cctcgtccac ctcgatgcgc gggaagaggg cctcaccgcg cgtggtccgc
     1621 gtccccggcc gcagaccgcc ccaggccgcg gcgcccggca gcacctgctc ggcgagcggc
     1681 gcctcgatcc cgagcgaggc ccacagcttc gcgctcgtcc ccggcatcac cggcgccgtc
     1741 aggatcgcca ggatgcgcaa cgactcgagg ccggcgtaga tcgcgcgggc cacgtcgtcg
     1801 cggcgcgcgg ggtccttcgc cagggcccac ggcgccacct cgaccaggta gcggttgacc
     1861 tccttgacga agtcccacac ggcggcgatg ccctcggtca ggtcgagcgc cagcatccgc
     1921 tcgtcgtagc gcccggcggc tgccgccgcg acctcggcca ggcgcgactc cgggcccggc
     1981 ccggccggcg gctcggggac catgccgtcg aagtaggcgg cggtcatggc cagtacccga
     2041 ctggcgaggt tcccgtagtc gttcgccagg tcggcgttgt accgcgcgac catggactcc
     2101 cacgagaagg agccgtccgc gccgaactgc acctcgcgca ggaagtagta gcggtaggcg
     2161 tcggtcccga actggtcgat cagctcgagc gggtgaatgc cggtcagctt ggtcttgctc
     2221 at
//
LOCUS       DASPWO010000592         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_82928, whole genome shotgun sequence.
ACCESSION   DASPWO010000592 DASPWO010000000
VERSION     DASPWO010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82928"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VEO00_13495"
     CDS             complement(<1..1456)
                     /locus_tag="VEO00_13495"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HYU59044.1"
                     /translation="MAGDERPITLTYTGPTLRQAADELAARKERARGMGGAERIERQH
                     GQGKLTVRERLDRLYDPGSFTEFALLAHHQSAAPAMQGKETPADGVVTGIGLVDGRRV
                     AVIAYDFTVMAGSIGMVGELKATRMRELALRERIPIVWLIDSAGARIQEAAGSMFART
                     GDLFKEQVHMSGVIPQVAAMMGPGAAGTAYIPGLADFVPMVKGTSHMALAGPHLVKAA
                     VGEDVTAEEMGGSAVHTKVSGVADLEVEGDDACLAAVRTYLSYFPSSNLEKPPVVETG
                     DPLDRRCEDLLDLVPANPRQAYDMRTVVASIVDRESFFPMKPGWARNLITGFARFGGR
                     PAGIVANQPMMLGGALDVNAADKAARFVWLCDAFHIPLVFLMDCPGFLVGSAVEKQGI
                     IRHGAKMLFAVAEATVPKVTIVLRKGYGAGYYVMNGRAYEPDLIVAWPTAEISVMGPE
                     GAVSIIFRKQVEAAPVEQRDEVRSTLVNAVREQIN"
     gene            complement(1551..2315)
                     /locus_tag="VEO00_13500"
     CDS             complement(1551..2315)
                     /locus_tag="VEO00_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59045.1"
                     /translation="MASPRQPTAQPSLVHVLEPILLDDGRGTPDRGTLEAGRGFLEFR
                     GARGTLRMAPVADVTRSGDGVVVAYGDTGTSRAVLSDLRRGPERAASRAGPFAALLRD
                     FVGLPPIVPPRRRVAGYLWGLVLIGWFAGYLVGVAFSGGGLGITDFAETVWTGGAGTV
                     AALAIAWSMGRPVRLAAAFRYGFAGATVASGFLIGVAGGEELADEGPDDLGAWLGSRA
                     GIAGLILLGVVLATATATSFLRFPTRAAGSGRASPA"
BASE COUNT          362 a          962 c          847 g          310 t
ORIGIN      
        1 ggttgatctg ctcgcggacg gcgttcacca gggtgctgcg cacctcatcg cgctgctcca
       61 ccggcgccgc ctcgacctgc ttgcggaaga tgatgctcac cgcgccctcc ggccccatca
      121 ccgagatttc cgcggtcggc caggcgacga tcaggtcggg ctcgtacgcg cggccgttca
      181 tcacgtagta cccggcgccg tagcccttgc gcagcacgat cgtcaccttc ggcaccgtcg
      241 cctcggccac cgcgaacagc atcttggccc cgtggcggat gatcccctgc ttctcgaccg
      301 ccgaacccac caggaacccc gggcagtcca tcaggaacac cagcgggatg tggaacgcgt
      361 cgcacagcca gacgaaccgc gcggccttgt ccgcggcgtt cacgtcgagc gcgccgccga
      421 gcatcatcgg ctggttggcc acgatccccg ccggacgccc cccgaaccgc gcgaagccgg
      481 tgatcaggtt gcgagcccag cccggcttca tcgggaagaa cgactcccgg tccacgatcg
      541 aggcgaccac cgtgcgcatg tcgtaggcct ggcgcgggtt cgccggcacc aggtcgagca
      601 ggtcctcgca gcgccggtcg agggggtcgc cggtctcgac cacgggcggc ttctcaaggt
      661 tcgacgacgg gaagtacgag aggtaggtgc gcacggcggc caggcaggcg tcgtcgccct
      721 cgacctccaa gtcggccacg ccggagacct tcgtgtgcac ggcggaaccg cccatctcct
      781 cggcggtgac atcctccccg accgcggcct tcaccaggtg cgggcccgcg agtgccatgt
      841 gcgaggtccc cttgaccatc gggacgaagt cggccaggcc cgggatgtac gccgtgcccg
      901 cggcgccggg gcccatcatc gccgcgacct gcgggatcac cccgctcatg tggacctgtt
      961 ccttgaacag gtcgccggtg cgggcgaaca tcgagccggc cgcctcctgg atccgcgcgc
     1021 cggccgagtc gatcagccag acgatcggga tccgttcgcg cagcgccagc tcgcgcatgc
     1081 gggtcgcttt cagctcgccg accatgccga tcgacccggc catcaccgtg aagtcgtagg
     1141 cgatcaccgc gacgcggcgg ccgtcgacga gcccgatccc ggtgaccacg ccgtcggccg
     1201 gcgtctcctt gccctgcatc gccggcgccg ccgactgatg gtgggccagc agcgcgaact
     1261 ccgtgaacga cccggggtcg tagaggcggt cgagccgctc gcggacggtc agcttgccct
     1321 gcccgtgctg ccgctcgatc cgctcggcgc cgcccatccc gcgggcccgt tccttgcgcg
     1381 ccgccagctc gtcggccgcc tggcgcaggg tcggccccgt gtaggtcagg gtgatcggcc
     1441 gctcgtcgcc ggccatgggg aacctccttc gcggcccttc gccgggagcg aacctcccga
     1501 tgcctgcccg ggctcgtcgc ccgagatcgt acgctcgccg gagcgagcga tcaggccgga
     1561 gacgctcgcc cgctcccggc cgcccgggtc gggaaccgga ggaacgaggt cgcggtcgcg
     1621 gtggccagca cgacgccgag gaggatcagc ccggcgatcc ccgcccgcga gccgagccac
     1681 gcccccaggt cgtccgggcc ctcgtcggca agctcctctc ccccggcgac gccgatcagg
     1741 aagcctgagg cgaccgtcgc ccccgcgaac ccgtaccgga aggcggcggc cagccggacc
     1801 gggcggccca tcgaccaggc gatggccaac gctgcaacgg tgcccgcgcc gccggtccaa
     1861 acggtctccg cgaagtcggt gatccccagc ccgccgccgc tgaaggccac gccgaccagg
     1921 tacccggcga accatccgat cagcacaaga ccccacaggt acccggcgac gcgccggcgc
     1981 gggggcacga tcgggggaag gccgacgaag tcgcgcagca gcgccgcgaa cggcccggcg
     2041 cgggaggccg cgcgctcggg tccgcggcgc aggtccgaga gcaccgcccg ggaggtcccg
     2101 gtgtcgccat aggccacaac cacgccgtcc ccggagcgcg tgacgtcggc gacgggcgcc
     2161 atccgcaggg tcccgcgcgc accgcggaac tccaggaacc ccctcccggc ctcgagcgtg
     2221 ccccggtccg gcgtcccccg gccgtcgtcc aggaggatcg gctcgaggac gtggaccagc
     2281 gacggctgcg cggtgggctg ccggggggag gccacgaccg cctaccccgg aggcgtcggc
     2341 ccgcccgact cccgctgcgc cttcgacccc ttcaggtact gggccaggcc gacgaagaag
     2401 tacaccagcc cccacagcat cgcgccccag gcgacgaagt aggtcccgcc gccggaggat
     2461 gctcgcgagt aggtgatcac g
//
LOCUS       DASPWO010000593         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_931929, whole genome shotgun sequence.
ACCESSION   DASPWO010000593 DASPWO010000000
VERSION     DASPWO010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931929"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VEO00_13505"
     CDS             <1..464
                     /locus_tag="VEO00_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59046.1"
                     /translation="ASWVENTWGGCALEAHPEVRERFGRRFGLPELAPDASPLGRVAW
                     EVGLFTSVLEAAADEHPQWHGAGHEDLCVDPHQKFRQLYERLSLTWTEAADRFLDEAN
                     RPGTGYIINRIAAEQPERWRRRLSTEQLEEVWSVLSRFDAPWVSRVAADIR"
     gene            complement(465..2051)
                     /locus_tag="VEO00_13510"
     CDS             complement(465..2051)
                     /locus_tag="VEO00_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-coumarate--CoA ligase family protein"
                     /protein_id="HYU59047.1"
                     /translation="MIIRSPHPDVTIPDVPLTEFVLGESPGQGDKAALIDGPTGRTLT
                     YSQLAEAVRRVAGGLAERGFGRGDVLGIFCPNVPEFAVTFHAVASLGGISTTINSLYT
                     PDEVAWQLKDAGAKVLVTVAPFMDRAADAAGRTGIQEVFVLGDGSGSDATPFAALLDT
                     DGSAPQPDFDPREDLVVLPYSSGTTGFPKGVMLTHHNLVSNLTQMKPFHHVTPEDRVI
                     GVLPFFHIYGMVVIMNGVLHNGGTVVTMPRFDLEQFLGLMQEHRITRAYLVPPIVLAL
                     AKHPLVDRYDLSSLRLILSGAAPLDAGLASACEQRLGCQVIQGYGLTETSPVTNCTPD
                     DDPSGNKPGSIGPLLPNTEARIVSFETGRDETPGQDGEIWIRGPQVMKGYLNNPEATR
                     ATIDEDGWLHTGDIGHADEDGYFTIADRLKELIKYKGYQVPPAELEAVLLSHPAISDA
                     AVIPKRDDEAGEVPKAFVVIKAEATADEIMIYVAERVALHKKIREVEFVEEIPKSASG
                     KILRRVLVDRERERAAQGAG"
     gene            2188..3627
                     /locus_tag="VEO00_13515"
     CDS             2188..3627
                     /locus_tag="VEO00_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYU59048.1"
                     /translation="MTRGSPRRVAIAVCTTLVAALLALPAGAATGSTAGSGSAKVDWL
                     MYGFDQQRTGYNPAETTIGVGNAGDLHPLWSVQMDSVVETSPVLATDVMVEGSPRDLL
                     YVGDEHGNFLAIDANTGEVIWNRNLGSVNAGCPYPPDRIFGVTAAAAIDRATGLIYEA
                     GGDGKMYAMDLATGAVQPGWPLTITTDPVHEHVWSAVTLFQGRVYVEIASYCDIGQYL
                     GRVVSFSAAQARRGPIFWVTGQGGPRGGGIWGWGGASLDPSNGNLYVATGNAFANPES
                     YAYAEHVVRLGPALRVKDSNYPGINGFDVDFGSTPVLYQAPGCPPALVTQNKAGALMV
                     YDPDAIGPGPVQRIQVGPNDLLFIGVPAYDPVTNMVYVVSASDSSGVYKHGLIAFRVQ
                     PNCALSLAWQQSRGPNRNNTASPTAANGVVYYGDGTGDQLFAFDAATGQELWNSGAII
                     DDEIHGTPIVVNGKLYAVSWDHRVYAFGP"
     gene            complement(3647..>3845)
                     /locus_tag="VEO00_13520"
     CDS             complement(3647..>3845)
                     /locus_tag="VEO00_13520"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYU59049.1"
                     /translation="PVVANGVVYLADGSNPKVYAFDALTGAPLWNSGTAVAQGFSSAP
                     IVVNGMLFVAGNDNKIHAFGL"
BASE COUNT          599 a         1321 c         1321 g          604 t
ORIGIN      
        1 tcgccagctg ggtcgagaac acgtggggcg gctgcgcact cgaggcgcac ccggaggtcc
       61 gagagcggtt cggtcggcgg ttcggcctcc ccgagctcgc tcccgacgcc tccccactgg
      121 gccgggtggc ctgggaggtg ggcctgttca cctcggtgct cgaggcggcg gccgatgagc
      181 acccccagtg gcacggcgcc ggccacgagg atctctgcgt ggacccgcac cagaagttcc
      241 gacagctcta cgagcgcctg tcgctgacct ggaccgaggc ggccgatcgg ttcctggacg
      301 aggcgaaccg gccgggcacc ggctacatca tcaaccgcat cgcggccgag cagcccgaac
      361 ggtggcgccg gcggctgagc accgaacagc tcgaggaggt ctggtccgtc ctgtcccggt
      421 tcgacgcgcc gtgggtctcg cgggtcgccg cggacatccg gtgatcagcc ggcgccctgc
      481 gcggcccgtt cccgctcccg gtcgaccagg actcgcctga ggatcttgcc cgaggcggac
      541 ttcgggatct cctcgacgaa ctcgacctcc cggatcttct tgtggagggc gacccgttcg
      601 gcgacgtaga tcatgatctc gtcggcggtg gcctcggcct tgatgaccac gaacgccttc
      661 gggacctcgc cggcctcatc gtcccgcttg gggatcaccg ccgcgtccga gatggcggga
      721 tgcgacagca ggaccgcctc gagctcggcc ggcgggacct ggtagccctt gtacttgatg
      781 agttccttga ggcggtcggc gatcgtgaag tagccgtcct cgtccgcgtg gccgatgtcc
      841 ccggtgtgga gccagccgtc ctcgtcgatc gtggcccgcg tggcctccgg gttgttgagg
      901 taccccttca tgacctgcgg cccccggatc cagatctccc cgtcctggcc gggggtctcg
      961 tcccgtcccg tctcgaagct cacgatccgg gcctccgtgt tcgggagcag gggaccgatc
     1021 gatccgggct tgttccccga cgggtcgtcg tcgggggtgc agttggtgac cgggctcgtc
     1081 tcggtcagcc cgtagccctg gatcacctgg cagcccagcc gttgctcgca cgccgacgcc
     1141 agacccgcgt cgagcggcgc ggcgccggag aggatcagcc gcaagctgga aaggtcgtac
     1201 cggtcgacca gcgggtgctt ggccagcgcc aacacgatcg gcggcacgag gtaggcccgc
     1261 gtgatccggt gctcctgcat gagaccgagg aactgctcca ggtcgaatcg gggcatggtc
     1321 accaccgtgc cgccgttgtg gagcaccccg ttcatgatca cgaccatccc gtagatatgg
     1381 aagaacggga ggaccccgat gacccgatcc tcgggcgtca cgtggtggaa cggcttcatc
     1441 tgcgtgaggt tggagaccag gttgtggtgg gtgagcatca cccccttcgg gaagccggtc
     1501 gtcccgctcg aatagggcag caccaccagg tcctcgcggg ggtcgaagtc aggctggggg
     1561 gccgaaccgt cggtgtcgag cagggcggcg aacggcgtgg cgtccgaacc agagccatcc
     1621 cccaggacga agacctcctg gatccccgtc cggcccgcgg cgtcggcggc ccgatccatg
     1681 aacggggcca cggtcacgag caccttcgca ccggcgtctt tcagctgcca cgcgacctcg
     1741 tcgggggtgt agagcgaatt gatcgtggtg ctgatgccgc cgagggaggc cacggcgtgg
     1801 aacgtgaccg cgaactcggg aacgttcggg cagaagatgc ccagcacgtc gccccggccg
     1861 aaaccccgtt cggcaagccc gcccgcgacc cgccggacgg cctctgccag ctgcgaatag
     1921 gtcagggtcc ggccggtcgg cccgtcgatc agcgccgcct tgtctccctg accgggggac
     1981 tcgcccagca cgaactccgt caaggggacg tccgggatcg tcacgtcggg atggggactc
     2041 cggatgatca tcgccgtcct cgctttcgga tctgatcctg agcggagcct agttcggggg
     2101 gccacgatgt gcgacccctc cagatgagcg tcccacggca accggtactg gcatacagtg
     2161 gcgatcgacc gagcgagggg gaacggaatg acgaggggat caccgcgtcg cgtcgcgatc
     2221 gccgtctgca ccacactggt tgctgccctg ctggcgctcc ccgccggggc cgccaccgga
     2281 tcgaccgcgg ggagcggttc ggcgaaggtc gactggctca tgtacggctt cgaccagcag
     2341 cgaaccggat acaaccccgc cgagacgacc atcggcgtcg gcaacgccgg agacctccat
     2401 ccactgtggt ccgttcagat ggactcggtg gtcgagacgt cgccggtgct ggccacggac
     2461 gtcatggtcg aggggtctcc ccgcgatctc ctctacgtgg gcgacgagca cggcaacttc
     2521 ctggccatcg atgcgaacac cggcgaggtg atctggaacc ggaacctcgg atcggtcaac
     2581 gcgggatgcc cctatcctcc ggaccggatc ttcggcgtga ccgcggcggc ggcgatcgac
     2641 cgcgccaccg gcctgatcta cgaggcgggc ggcgacggga agatgtacgc gatggacctc
     2701 gcgacgggcg ccgtccagcc ggggtggccg ctgacgatca ccaccgaccc ggtccacgag
     2761 cacgtgtgga gcgcggtgac cctgttccag ggccgggtct acgtcgagat cgccagctac
     2821 tgcgacatcg gacagtacct gggccgggtc gtctcgttct ccgcggcgca ggcccgtcgc
     2881 ggccccatct tctgggtgac gggccagggc ggtccccggg gcggcgggat ctggggctgg
     2941 ggcggagcgt cgctcgaccc gtccaacggc aacctgtacg tggccacggg aaacgccttc
     3001 gcgaaccccg agtcatacgc ctacgcggag cacgtcgtgc ggctcggccc cgccctccgg
     3061 gtgaaggatt cgaactatcc cgggatcaac gggttcgacg tcgacttcgg ctccacgccg
     3121 gtgctctatc aggcgccggg gtgtccgccc gcgctggtca cgcagaacaa ggccggtgcg
     3181 ctgatggtct atgacccgga cgccatcggg ccgggcccgg tccagaggat ccaggtcggc
     3241 ccgaacgacc tcctcttcat cggggttccg gcgtacgacc cggtcaccaa catggtctac
     3301 gtcgtgagcg cctcggattc gagcggcgtg tacaagcacg ggctgatcgc cttccgggtt
     3361 cagccgaact gcgcactgtc gcttgcctgg caacagagcc gagggccgaa ccgcaacaac
     3421 accgcctcgc cgaccgccgc caacggggtc gtctactacg gagacggcac cggcgatcag
     3481 ctgttcgcat tcgacgccgc gaccggacag gagctctgga acagcggagc catcatcgac
     3541 gacgagatcc acggaacgcc gatcgtggtc aacgggaaac tgtacgcggt gtcgtgggac
     3601 caccgcgtgt acgccttcgg gccgtagccc tcaccgaggg gccgggctac agaccgaacg
     3661 cgtggatctt gttgtcgttt cccgcgacga acagcatgcc gttcacgacg atgggcgccg
     3721 agctgaaccc ctgggccacg gccgtcccac tgttccacaa tggcgcgccg gtcagcgcgt
     3781 cgaacgcgta gaccttgggg ttcgacccgt ccgccaggta caccacgccg ttcgccacga
     3841 ccggc
//
LOCUS       DASPWO010000594         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1183950, whole genome shotgun sequence.
ACCESSION   DASPWO010000594 DASPWO010000000
VERSION     DASPWO010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183950"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1233)
                     /gene="aroB"
                     /locus_tag="VEO00_13525"
     CDS             complement(154..1233)
                     /gene="aroB"
                     /locus_tag="VEO00_13525"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HYU59050.1"
                     /translation="MRRVPVRAGSGAYDVIVGPGLLEEGAILPELPGAEKAFLVTDAT
                     VGPLYVEPLSRTLAGAGLATVTLTVAPGEPSKSLATAEALYQQLAVQEAHRDDPLFAL
                     GGGVVGDLAGFVAATYMRGVPLVQVPTTLTAQVDAAIGGKTGINLARGKNLVGAYHQP
                     RAVIADVATLATLPEPEFRSGLGEVAKYGFALDPGVLGILEASLGAILARDPSVLEDL
                     VARCAAAKAAVVSEDERDAGRRLVLNYGHTLGHALERIDAFRGRSHGEAVALGMAFAA
                     RLAADLGVGPVDLVGRHRRVLASLGLPHGTQGVATEQVLEAMRMDKKYRGGVRFVLLE
                     AAGRPRIVADVPEDAVARAMEGLRA"
     gene            complement(1241..1711)
                     /locus_tag="VEO00_13530"
     CDS             complement(1241..1711)
                     /locus_tag="VEO00_13530"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HYU59051.1"
                     /translation="MHGSGKSTVGRLLAERLGAEFLDLDEEIEASEGRAVEEIFAAGG
                     EETFRDRESAALMRTVGLTNAVVACGGGVVLRQANRRVLSAGRAVVWLRVPEEELARR
                     VGDGEGRPLLVAGGDLAALAAAREGIYAQVATITVNGEADPATLAAAVAERLVG"
     gene            complement(1732..>2795)
                     /gene="aroC"
                     /locus_tag="VEO00_13535"
     CDS             complement(1732..>2795)
                     /gene="aroC"
                     /locus_tag="VEO00_13535"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009377148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HYU59052.1"
                     /translation="ELLAGVRFGRTLGSPIAITVANSEWEKFRERMDPEGDPVEERLT
                     RPRPGHADLAGMLKFGFDDARNILERASARETAARVAAGVACKAFLGELGISVLSHVV
                     AIGNVRARARRLPAPADREAIDASPVRTFDADVETRMVGAIDAARKARDTLGGVFEVL
                     VHGAPPGLGSHVQHDRKLDARLALALMSVQAIKGVEVGDGFASAARPGSRAHDEIVAR
                     GGRLSRATGRAGGIEGGTSTGEVIRIRAAMKPLSSLPRPLATVDMDTGERAVAIRQRT
                     DTCAVPAAAVVGEAVVALVIADAVLEKFGGDSVAETRRNLEAFLASLPFSMGGPPAGG
                     GRVDRTTGRATPPSPAPGS"
BASE COUNT          355 a         1105 c          993 g          342 t
ORIGIN      
        1 cgtggtcgga ctgggccacc tcgacggcgt ggccgtcggc gtcggcggcc gctctcagct
       61 cagcggtcag gtcggcgagc gtggccgagc cgtagcgctc ggggtcgcgc cggccgagcg
      121 cgccgaggtt cggcccgagc aggacgaggg ccttcacgcg cgcaggcctt ccatggcgcg
      181 ggccaccgcg tcctcgggca cgtcggcgac gatccgcggc cgcccggctg cctcgagcag
      241 gacgaatcga acgccgcccc ggtacttctt gtccatccgc atcgcctcga gcacctgctc
      301 ggtcgccacc ccctgggtcc cgtgcggcag gccgagcgag gcgagcacgc gccggtgccg
      361 cccgaccagg tccaccggcc ccaccccgag gtcggcggcc agccgggcgg cgaacgccat
      421 cccgagcgct accgcctcgc catgcgagcg tccccggaac gcgtcgatcc gctcgagcgc
      481 gtggccgagg gtgtgcccat agttcagcac cagccgccgt ccggcgtcgc gctcgtcctc
      541 ggacaccacg gcggccttcg ccgcggcgca gcgcgcgacc aggtcctcca gcaccgaagg
      601 gtcgcgcgcc aggatcgcgc cgagcgaggc ctccaggatg ccgagcacgc ccgggtcgag
      661 ggcgaacccg tatttcgcca cctccccgag accggagcgg aactccggct cgggcagcgt
      721 tgcgagcgtg gcgacgtcgg cgatcaccgc gcgcggctgg tggtaggcgc cgaccaggtt
      781 cttcccgcgt gccaggttga tcccggtctt gcccccgatc gcggcgtcca cctgcgccgt
      841 cagcgtggtc gggacctgca ccagcggcac cccgcgcatg taggtcgcgg cgacgaaccc
      901 ggccaggtcg ccgaccaccc cgccaccgag cgcgaacagg gggtcgtcgc gatgcgcctc
      961 ctgcacggcg agctgctggt agagcgcctc ggccgttgcg agcgacttgc tcggctcgcc
     1021 gggggccacg gtcagcgtga ccgtcgccag accggcgccg gcgagcgtcc gcgagagcgg
     1081 ctcgacgtag agcgggccaa ccgtggcgtc ggtgacgagg aaggccttct ccgcgccggg
     1141 cagctcgggg agtatcgcgc cctcctcgag cagccccggc cctacgatca cgtcgtaggc
     1201 gccactgccc gcacggaccg ggacgcgtct catcgccgcc tcacccgacc agccgctcgg
     1261 cgacggcggc ggccagcgtc gccgggtcgg cctctccgtt cacagtgatg gtcgccacct
     1321 gcgcgtagat cccctcgcgc gccgcggcga gcgcggcgag gtcgcccccg gccaccagca
     1381 gcggccgccc ctcgccgtct ccgacccggc gggccagttc ctcctccggc acgcgcagcc
     1441 agaccaccgc ccggccggcc gagaggacgc ggcggttcgc ctggcgcaac accaccccgc
     1501 ccccgcaggc gaccaccgcg ttcgtcaggc ccaccgtgcg catcagcgcg gcggactcgc
     1561 gatcgcggaa cgtctcctcg ccgccggccg cgaagatctc ctcgaccgcg cggccctcgg
     1621 acgcctcgat ctcctcgtcg aggtcgagga actcggcgcc caggcgttcc gcgagcagcc
     1681 gcccgacggt cgacttcccc gatccgtgca tcccgacgag gtacacgggc ctcacgagcc
     1741 cggcgcgggg cttggggggg tagctcgccc ggtggtgcga tccacccgac cacctccggc
     1801 gggcggaccc cccatggaga atgggaggga ggccaggaag gcctccaggt tgcggcgagt
     1861 ctcggccacc gagtcgccgc cgaacttctc caggaccgcg tcggcgatga cgagggcgac
     1921 caccgcctcg ccgaccacgg ccgccgctgg gacggcgcag gtgtcggtgc gctggcggat
     1981 ggccaccgcc ctctcaccgg tgtccatgtc cacggtggcg agcgggcggg gcagcgacga
     2041 gaggggcttc atggccgcgc ggatgcggat cacctcgccg gtgctcgtcc cgccctcgat
     2101 gccgccggcg cgcccggtgg cgcgggacag gcggccgccg cgggccacga tctcgtcgtg
     2161 cgcgcgcgag cccggccgag cggccgaggc gaacccgtca ccgacctcga cccccttgat
     2221 cgcctgcacg ctcatcagcg ccagcgccag tcgcgcgtcg agcttgcggt cgtgctggac
     2281 gtgcgagccg agccccggcg gcgcgccgtg cacgagcacc tcgaacacgc cgccgagcgt
     2341 gtcgcgggcc ttccgggcgg cgtcgatcgc cccgaccatc cgcgtctcga cgtcggcgtc
     2401 gaacgtccgg accggggagg cgtcgatcgc ctcccggtcg gccggcgccg gcagacggcg
     2461 cgcgcgcgcg cgaacgttcc cgatcgccac gacgtgtgac aggacggaga tcccgagttc
     2521 gccgagaaag gccttgcagg ccaccccggc cgcgacgcgg gccgcggtct cgcgcgcgct
     2581 ggcccgctcc aggatgttgc gggcgtcatc gaatccgaac ttcagcatcc ccgccaggtc
     2641 ggcatgcccg ggccggggcc gggtcaggcg ctcctcgacc ggatcgccct ccgggtccat
     2701 ccgctcgcgg aacttctccc attcgctgtt ggcgaccgtg atcgcgatcg ggctcccgag
     2761 cgtccggccg aaccggaccc cggccagcag ctcgg
//
LOCUS       DASPWO010000595         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_253741, whole genome shotgun sequence.
ACCESSION   DASPWO010000595 DASPWO010000000
VERSION     DASPWO010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253741"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VEO00_13540"
     CDS             complement(<1..353)
                     /locus_tag="VEO00_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HYU59053.1"
                     /translation="MPVEAVVFDLDGVLIDSEPVWREVEARVAEEFGFAYSDELAVRM
                     HGRGTIGAGRIIAELTGDEASGLAASERMVELMVERGSAGRIPPMPGARELLETLRGR
                     VRLAVASNSVRRYVEG"
     gene            complement(359..1837)
                     /gene="gcvPB"
                     /locus_tag="VEO00_13545"
     CDS             complement(359..1837)
                     /gene="gcvPB"
                     /locus_tag="VEO00_13545"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HYU59054.1"
                     /translation="MGARRVGAPVRTAGPAVPATLRELSRPGRRAFTLPTLDVPEAAV
                     AEEHRRTTPPGLPEVGERDLVRHFTRLAQMNYGVDTGFYPLGSCSMKYNPKVAEEVAA
                     LPGFRRLHPLQPESTVQGALALVHALESMLCEVTGMARVTFQPAAGASGELTGLLIMR
                     GHHARNGDPRRRVVIPDSAHGTNPASVTLAGYEVAEVPSDARGLVDVSALEKLMGEDV
                     AGLMITNPNTLGLFEEDILDIARIVHDAGGLLYYDGANLNAIMGVVRPGDMGFDIVHM
                     NTHKTFATPHGGGGPGAGPVAVAERLVPFLPSPTVEYDEAANRHYLDHDRPLSIGRVH
                     GFHGNFGVLVRAYAYVVAHGGDGLRAVAERAVLNANYLCERVKGRFPLAFSGRPMHEF
                     VATAKPLRKHGIRATDVAKRLIDLGFHPSTVYFPLVVEEALMVEPTETESRETLDAFA
                     EAMLQAAREAESDPDLLHQAPVTAPVRRLDEARAARELKLHW"
     gene            complement(1852..>2400)
                     /locus_tag="VEO00_13550"
     CDS             complement(1852..>2400)
                     /locus_tag="VEO00_13550"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935222.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HYU59055.1"
                     /translation="EGQSLGNHLNFGGPYLGVIAARMQDVRRLPGRIAGETLDADGNV
                     GYVLTLQAREQHIRREKATSNICTNQTLMAIAAAAYLGWLGPRGLEELGGLCASRAAY
                     AAERLTAIEGVDLLHPDAPFFKEFALHLPRDPRQVVEAMVGRGYLAGVPIEDDALLVA
                     VTERRTKREIDGFAEALREVLS"
BASE COUNT          340 a          902 c          826 g          332 t
ORIGIN      
        1 ccctccacgt agcggcggac cgagttcgac gcaacggcca gccggacgcg gccgcggagc
       61 gtctcgagca gctcgcgcgc cccgggcatc ggcgggatcc tccccgcgga cccgcgctcg
      121 accatcagct ccaccatccg ctcggacgcg gcgagcccgc tcgcctcgtc gcccgtcagc
      181 tccgcgatga tccgaccggc gccaatcgtc ccgcggccgt gcatccgcac ggccagctcg
      241 tcggagtacg cgaacccgaa ctcctccgcc acgcgcgcct cgacctcccg ccacaccggc
      301 tcggagtcga tcaacacccc gtcgaggtcg aacacgaccg cctcgaccgg caatcgggct
      361 accagtggag cttcagttcg cgcgcggcgc gagcctcgtc caggcggcgg acgggcgcag
      421 tgaccggcgc ctggtgcagg aggtcgggat ccgattcggc ctcgcgggcc gcctgcagca
      481 tcgcctcggc gaatgcgtcg agcgtctcgc gcgactcggt ctcggtcggc tcgaccatca
      541 gcgcctcctc gaccaccagc gggaagtaca ccgtcgaagg atggaagccc aggtcgatca
      601 gccgcttcgc gacgtctgtc gcccggatcc cgtgcttcct caatggcttg gcggtggcca
      661 cgaactcgtg catcggacgg cccgagaacg ccagtgggaa tcgccccttc acccgctcgc
      721 agaggtagtt ggcgttcagc acggcacgct ctgcgaccgc gcgaaggccg tcgccgccgt
      781 gggcgaccac gtacgcgtag gcccggacga gcacaccgaa gttgccgtgg aagccgtgca
      841 cgcgcccgat cgagagcggg cgatcgtggt ccaggtagtg ccggttggcc gcctcgtcgt
      901 actcgacggt cggcgacggc aggaacggga ccaggcgctc cgccaccgcc accgggccgg
      961 cccccggccc gccgcctcca tgcggtgtgg cgaaggtctt gtgtgtgttc atgtgcacga
     1021 tgtcgaagcc catgtcgccg ggccggacca cgcccatgat cgcgttcagg ttggcgccgt
     1081 cgtagtagag gaggcccccg gcgtcgtgca cgatccgcgc gatgtccagg atgtcctcct
     1141 cgaacagccc gagcgtgttc ggattcgtga tcatcaggcc ggcgacgtcc tcgcccatca
     1201 gcttctcgag cgccgagacg tctaccaggc cgcgggcgtc gctcggcacc tcggccacct
     1261 cgtagccggc gagcgtcacc gaggccgggt tcgtgccgtg cgccgagtcc gggatcacga
     1321 cgcgccggcg agggtcaccg ttccgggcgt ggtgcccgcg catgatcaac agcccggtca
     1381 gctcgcccga ggctcccgcc gccggctgga acgtgacccg ggccatgccg gtcacctcgc
     1441 acagcatcga ctccagggcg tggaccaggg ccagcgcccc ctggaccgtc gactcgggct
     1501 gcagcggatg gagccggcgg aaccccggca gcgcggcgac ctcctccgcg accttcgggt
     1561 tgtacttcat cgagcaggag ccgagcgggt agaagccggt gtcgaccccg tagttcatct
     1621 gcgccagccg ggtgaagtgg cgcaccaggt cccgctcccc cacctcgggc agtccgggcg
     1681 gcgtcgtccg gcggtgctcc tcggcaacgg cggcctcggg cacgtcgagc gtcggaagcg
     1741 tgaaggcgcg gcgcccgggg cgagacagct cccggagcgt cgccgggacc gccgggccgg
     1801 ccgtgcgcac cggggcgccg acccgccgcg cgcccatcgc tgcctcctcc gtcacgagag
     1861 cacctcgcgc agggcctcgg cgaacccatc gatctcacgc ttcgtccgcc gctcggtgac
     1921 cgccaccagc agggcgtcgt cctcgatcgg gacaccggcc aggtagcccc ggccgaccat
     1981 cgcctcgacc acctggcgcg ggtcgcgcgg caggtgaagc gcgaactcct tgaagaacgg
     2041 cgcgtcggga tgcaggaggt cgacaccctc gatcgcggtc agtcgctcgg cggcgtacgc
     2101 ggcgcgcgac gcgcacagcc caccaagctc ctccagcccg cgcgggccga gccagccgag
     2161 gtaggcagcg gcggcgatcg ccatcagcgt ctggttggtg cagatgttcg acgtggcctt
     2221 ctcgcggcgg atgtgctgct cgcgggcctg gagcgtcagc acgtagccca cgttgccgtc
     2281 ggcgtcgagc gtctcgccgg cgatccggcc ggggagccgg cggacgtcct gcatccgcgc
     2341 ggcgatcacc ccgaggtacg gtcccccgaa gttcaagtgg ttgccgagcg actgtccctc
//
LOCUS       DASPWO010000596         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_506690, whole genome shotgun sequence.
ACCESSION   DASPWO010000596 DASPWO010000000
VERSION     DASPWO010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506690"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VEO00_13555"
     CDS             <1..639
                     /locus_tag="VEO00_13555"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HYU59056.1"
                     /translation="SKPAAVVVHPSGAVRSGTLVNRLLGAGIRLSSGSEPDRPGIVHR
                     LDAGTSGLMVVAKDDAAHAALRALFASHGADRRYLALVRGAIEEETFGIDAPLARRAG
                     RTVVDPVGGAEARTEVRVLERHHRATLVEARPRTGRTHQIRVHLASIGHPVLGDRAYG
                     GAGEYARALGLDRPFLHSATLAFDHPVTGERIELHEPLPGDLSRALKRSRLT"
     gene            712..1140
                     /locus_tag="VEO00_13560"
     CDS             712..1140
                     /locus_tag="VEO00_13560"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYU59057.1"
                     /translation="MNQATAVGQKWFAAVEAGDIDRMAEMLADDVDFQIPGGQFGRRE
                     DAVEALRPFTTAFSDTSFTIDRWIEQEDVSVAEGIYRATHTDVLPTPMGDVPPSGNTV
                     SLPFVSIFGEREGRVALQHAYWDNATFMMQIGAMPGPDAG"
     gene            complement(1142..>2013)
                     /locus_tag="VEO00_13565"
     CDS             complement(1142..>2013)
                     /locus_tag="VEO00_13565"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HYU59058.1"
                     /translation="GREVLANYHREAVNDYRIPAALEFPFSIEVEGVTLSGVIDRLDR
                     IPGGGYELIDYKTNRRLPPMARVDRDLQLSIYWLAAREVWGIEPERLTLWYLLPGQRM
                     TTTRTAADAERLKARIATVAERIEAGRFEPRENPLCDWCDFQARCPLFRHRFEREAGD
                     PAPRITEAVDEWIALKRRSKEIWSRMEELSAVIHAFAEEHGYGKLFGSDGAAVARVAR
                     HDGEYDLEAVRDALQPLGMFEDVLTVDRAKLSALIEGRTLPPDVEDALLASKEDVRTV
                     YALYLRENARSRR"
BASE COUNT          274 a          722 c          735 g          282 t
ORIGIN      
        1 tcgaagccgg ccgccgtggt ggtgcacccc tcgggcgccg ttcgttccgg gacgctggtc
       61 aaccggctgc tcggcgccgg gatccggctg tcgagcgggt cggagccgga ccgccccggg
      121 atcgtccacc ggctcgacgc cggcacgtcc ggactgatgg ttgtggccaa ggacgacgcg
      181 gcccacgccg cgctgcgcgc cctcttcgcc agccacggcg ccgaccgccg ctacctggcg
      241 ctcgtccgcg gggcgatcga agaggagacc ttcggcatcg acgccccgct cgcccggcgg
      301 gcggggagga cggtcgtcga cccggtcggc ggcgccgagg cgcgcacgga ggtccgggtg
      361 ctcgagcggc accaccgcgc gaccctggtc gaggcccggc cgcgcaccgg gcgcacccac
      421 cagatccggg tgcacctcgc ctcgatcggg catccggtcc tgggcgaccg cgcctacggc
      481 ggtgccggcg agtacgctcg cgccctcggc ctggatcgtc ccttcctgca cagcgccacg
      541 ctcgccttcg accacccggt caccggcgag cgcatcgagc tccacgagcc cctcccgggg
      601 gacctctccc gcgccctgaa gcgctcccgc ctgacctgag ggtgccttgc cggacgcggc
      661 ggcggccgct accgtgtgcg gccgatccga tcgagcgaag gggaggacgg gatgaaccag
      721 gcgacggcgg ttgggcagaa gtggttcgcc gcggtggagg ccggggacat cgacaggatg
      781 gccgagatgt tggcggacga cgtcgacttc cagattcccg ggggacagtt cgggcgacgg
      841 gaggacgccg tggaggcgct gcgacccttc acgacggcgt tctcggacac gagcttcacg
      901 atcgaccggt ggatcgaaca ggaagacgtt tccgtggccg agggcatcta ccgggcgacc
      961 catacggatg tcctgcccac gcccatgggg gacgtccccc cgtcggggaa taccgtcagc
     1021 ctcccgttcg tgtcgatctt cggcgagcgc gaagggaggg tcgctctcca gcacgcgtac
     1081 tgggacaacg ccaccttcat gatgcagatc ggcgcgatgc ccgggcccga cgcgggctga
     1141 gtcagcggcg agaccgggcg ttctcccgga ggtagagcgc gtagaccgtg cggacgtcct
     1201 ccttcgaggc gaggagggcg tcctcgacgt cggggggcag cgtgcgcccc tcgatcagcg
     1261 cggagagctt cgcgcgatcg acggtcagga cgtcctcgaa catgccgagt ggctgcaggg
     1321 cgtcgcgcac ggcctcgagg tcgtactcgc cgtcgtggcg ggccacccgc gccaccgcgg
     1381 cgccgtcgga gccgaacagc ttcccgtagc cgtgctcctc ggcgaacgcg tggatcacgg
     1441 ccgacagctc ctccatccga ctccagatct ccttcgagcg ccgcttcagg gcgatccact
     1501 cgtccacggc ctcggtgatc cgcggcgccg ggtcgcccgc ctcccgctcg aagcggtggc
     1561 ggaacagcgg gcagcgcgcc tggaagtcgc accagtcgca cagggggttc tcgcggggct
     1621 cgaaccggcc ggcctcgatc cgctcggcca cggtggcgat ccgcgccttc agccgctcgg
     1681 cgtcggccgc ggttcgcgtg gtggtcatcc gctgcccggg gagcaggtac cagagcgtga
     1741 gccgctctgg ctcgatgccc cacacctcgc gggcggccag ccagtagatc gacagctgca
     1801 ggtcgcggtc cacccgcgcc atcgggggga ggcggcggtt ggtcttgtag tcgatgagct
     1861 cgtagccgcc gccggggatg cggtccaggc ggtcgatgac gcccgacagc gtgacgccct
     1921 cgacctcgat cgagaacggg aactcgagcg ccgccgggat gcggtagtcg ttcacggcct
     1981 cgcggtggta gttggccagg acctcccggc cgt
//
LOCUS       DASPWO010000597         4457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_164942, whole genome shotgun sequence.
ACCESSION   DASPWO010000597 DASPWO010000000
VERSION     DASPWO010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164942"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="VEO00_13570"
     CDS             complement(<1..1642)
                     /locus_tag="VEO00_13570"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYU59059.1"
                     /translation="MIREAFRGKRVVVTGTTGFLGTAVVEKLLRSVPEVGSIVLPVRA
                     WSADHAANRVRDEVVASPAFGPLREQAGEDRFVELVAKLEAVPMDITRDDGLPDPAPL
                     AGSDIAVHCAATVSFDPPVDEAFETNLKGSLRFFEWSRAAGVRRYVQVSTAYMVGIRR
                     GEVPEFLPTNDPEAVPVNWRTEIEAVAAARREVERVSRMPERLAEFTAQAKREMGPAG
                     VPAVARRAERIRTQWVTDRLVERGNARARALGFPDVYAMTKNLTERALVETRGDVELA
                     IVRPSIIESAIREPFPGWIRGFRVAEPIILAYGRGALEDFPGLPEGIIDIVPVDLAVN
                     CILAAAAAPIAPEDDPPAYHVVSGSRNPLRYGEAFGFVRDYFRDHPLLDEDGQPIDAP
                     DWKFPGQESIRTRLRRGTRALERAQRVLRFLPASGRVRDAVDAVERSRQRVQFLGRYA
                     NLYGPYAEFEAVFLDDRARALEDSLDDEERALFPFDPSGIDWAHYWGVLHLPAVAEGR
                     DAPRRRPPAGVVVERAPEGATVLAAFDLEGTVAATNVIE"
     gene            complement(1735..1929)
                     /locus_tag="VEO00_13575"
     CDS             complement(1735..1929)
                     /locus_tag="VEO00_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59060.1"
                     /translation="MFSWRYLDAGGRETGRSDPFTDREAAEAWMGESWQTLLAAGNEE
                     VALHDDERDRTVYRMGLREA"
     gene            2085..3245
                     /locus_tag="VEO00_13580"
     CDS             2085..3245
                     /locus_tag="VEO00_13580"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HYU59061.1"
                     /translation="MAAVVAALLVLPAPGAFAAWSDVPNNHWAKVAIDYVAADRDWMR
                     DYGASTFRPGTVETRLLLARAAVFAFDPSGTPDRAITFSDLPANDPLWRYANVAVKNG
                     WLQPNGAGAFRPDGAMTLNTLYRTIVLGLGLTGAVAGLKAIHLADGTPVPHTQHFPYL
                     QVGMTIGLRYNHPDEATDVLPGQALTRDEVAWALYRAATAPSWKLDEADRFAALTLPN
                     MTATKLQMVVFGLKSVGYPYVYAGEWNVASPPGYCCGYQPQGGFDCSGLVWWVVRSPG
                     GGYDNTAIRGYQGWSLPERSSSTMAAVGTHISWTAKAAGDLLFYTSPISHVDLYVGRG
                     WALDSSSSRGGVALQWIVDGWYFDEFQWGRRLSPGPSSPYRGLPPENLLDGG"
     gene            complement(3213..3578)
                     /locus_tag="VEO00_13585"
     CDS             complement(3213..3578)
                     /locus_tag="VEO00_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYU59062.1"
                     /translation="MSAKRILIIDNRPEHLRQPVLRLQVEGFDVEEAHSEDEGLRRLR
                     EVRFDLLLLDAEMPEGDGWETLRRIREDPELAALKVIIFMAGQGETGKLGLVPVDGEL
                     RRPFSMGQLLAAVQKVLGG"
     gene            3770..>4457
                     /locus_tag="VEO00_13590"
     CDS             3770..>4457
                     /locus_tag="VEO00_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59063.1"
                     /translation="MAAVSAALLATLAPGGHADPSKRDLDAARAELDGLNERLSLLVE
                     EYDVAKIEVERLETRLADLRENASRARAAYRAARQALAERAAAAYIQGPGQGLDVLFG
                     STSFGDFTARLEFLNRMSQRDSDLAARAEQRRQRLRFAEAELRPALASANAAVADLDG
                     KQAQIEAGIADQQALVAKIEAVLQRKLEARPKPPANPAAPTTDPGPAPGPLPTSGGAA
                     TAIAAARSMIG"
BASE COUNT          591 a         1674 c         1601 g          591 t
ORIGIN      
        1 actcgatgac gttcgtcgcg gccacggtcc cctcgaggtc gaacgcggcg agcaccgtcg
       61 ctccctcggg cgcccgctcg acgacgacgc ccgccggcgg gcgccgccgt ggggcgtccc
      121 ggccctccgc aacggccggc aggtgcagga cgccccagta gtgcgcccag tcgatgcccg
      181 aagggtcgaa cgggaagagc gcccgctcct cgtcgtcgag cgagtcctcg agcgcccggg
      241 cgcggtcgtc gaggaacacc gcctcgaact ccgcgtaggg cccgtacagg ttcgcgtagc
      301 ggcccaggaa ctggacgcgc tggcgggagc gctcgacggc gtccaccgcg tcgcggacgc
      361 ggccgctcgc cgggaggaac cgcaggacgc gctgggcgcg ttccagggcc ctggtgccgc
      421 ggcgcagtcg cgtgcggatg ctctcctgcc ccgggaactt ccagtcgggc gcgtcgatcg
      481 gctggccgtc ctcgtcgagc agcgggtggt cgcggaagta gtcgcggacg aacccgaacg
      541 cctccccgta ccgcagtggg ttccggctgc cggagaccac gtggtacgcg ggcgggtcgt
      601 cctcgggcgc gatcggcgcg gccgccgcgg ccaggatgca gttgaccgcc aggtccaccg
      661 ggacgatgtc gatgatcccc tccgggaggc cggggaaatc ctcgagcgcc ccgcgcccgt
      721 aggccaggat gatcggttcg gccacccgga agccgcggat ccacccgggg aacggctcgc
      781 ggatcgcgct ctcgatgatg gaggggcgga cgatcgccag ctcgacgtcg ccgcgggtct
      841 cgacgagggc gcgctcggtg aggttcttcg tcatcgcgta gacgtccggg aacccgaggg
      901 cgcgcgcccg ggcgttgccg cgctcgacga gccggtcggt gacccactgg gtgcggatgc
      961 gctcggcgcg gcgggcgacg gccgggacgc ccgccggccc catctcccgc ttcgcctgcg
     1021 cggtgaactc cgcgagccgc tcgggcatcc gcgacacgcg ctcgacctcc cggcgcgcgg
     1081 cggccaccgc ctcgatctcg gtgcgccagt tcaccgggac cgcctcgggg tcgttcgtcg
     1141 gcaggaactc cgggacctcg ccgcggcgga tcccgaccat gtaggcggtg gagacctgca
     1201 cgtagcgccg cacgccggcc gcgcgcgacc actcgaagaa gcgcaggctc cccttcaggt
     1261 tcgtctcgaa ggcctcgtcc accggcgggt cgaacgagac cgtcgccgcg cagtggacgg
     1321 cgatgtcgct gccggcgagc ggggccgggt cgggcaggcc gtcgtcgcgg gtgatgtcca
     1381 tcgggacggc ttcgagcttc gcgacgagct ccacgaagcg gtcctcaccg gcctgctcgc
     1441 gcagcgggcc gaacgccggg gaggcgacga cctcgtcgcg gacgcggttc gcggcgtggt
     1501 cggcagacca ggcgcggacc ggcagcacga tcgagcccac ctcggggacc gagcgcagga
     1561 gcttctccac caccgcggtc ccgaggaacc cggtcgtgcc ggtcaccacg acgcgcttgc
     1621 cgcggaacgc ctcgcggatc atgggggggt cgcccggcgc gtccacgccg gcgcggggaa
     1681 gctcgtcacg aagcgagatt ctagggcggc aggcgtcgtc gagtcagtcg gccctcaggc
     1741 ttcgcgcagg cccatccggt agaccgtccg gtcgcgctcg tcgtcgtgga gcgcgacctc
     1801 ctcgttgccc gcggcgagca gcgtctgcca cgactcgccc atccacgctt cggccgcctc
     1861 gcggtcggtg aacgggtcgg agcggccggt ctcccgcccg cccgcgtcga ggtagcgcca
     1921 cgagaacatc accgctaggg tggccgaggc gctcaaggac agcaagcccg gcgaccgata
     1981 tgcaggttcc gatgcacacc acgccgagca ccgcatcgcg cgttccggcg cgggccgcac
     2041 cgatcgcccc ccggtccccg cggagcggtc gccggttcgc gctggtggcg gccgtcgtgg
     2101 ccgcgctcct cgtgctgccc gcgccgggcg ccttcgcggc gtggtcggac gtcccgaaca
     2161 accactgggc gaaggtggcc atcgattacg tcgccgccga ccgcgactgg atgcgcgact
     2221 acggcgcctc gacgttcagg cccggcacgg tcgagacccg cctgttgctc gcccgcgcgg
     2281 ccgtgttcgc cttcgacccg agcggcaccc cggatcgcgc gatcacgttc agcgacctgc
     2341 cggcgaacga cccgctgtgg cgctacgcga acgtcgcggt gaagaacggc tggctgcagc
     2401 cgaacggcgc cggcgcgttt cggccggacg gggcgatgac gctcaacacg ctctaccgca
     2461 cgatcgtgct cgggctcggg ctgacgggcg ccgtggccgg cctgaaggcg atccacctgg
     2521 ccgacgggac cccggtgccc cacacgcaac acttccccta cctccaggtc gggatgacga
     2581 tcgggctgcg ctacaaccac cccgacgagg cgaccgacgt gctgcccgga caggcgctga
     2641 cgcgcgacga ggtggcgtgg gcgctctacc gcgcggccac ggcgccgtcc tggaagctcg
     2701 acgaggcgga ccggttcgcc gccctcacgc tgccgaacat gacggcgacg aagctgcaga
     2761 tggtggtgtt cggcttgaag tccgtcggct atccgtacgt ctacgccggc gagtggaacg
     2821 tcgcctctcc gcccggctac tgctgcggct accagccgca gggaggcttc gactgctcgg
     2881 gcctggtctg gtgggtggtg cgctcgcccg gcggcgggta cgacaacacg gccatccgcg
     2941 gctaccaggg atggtcgctc ccggagcggt cgtcgtcgac gatggccgcg gtcgggaccc
     3001 acatctcctg gaccgcgaag gccgccggcg acctgctctt ctacacgagc ccgatcagcc
     3061 acgtggacct gtacgtcggg cgcggctggg ccctcgactc gtcctccagc cggggcggcg
     3121 ttgcgctgca gtggatcgtg gacggctggt acttcgatga attccagtgg ggccggcgcc
     3181 tgtcgccggg cccttcgagc ccgtaccggg ggctaccccc cgagaacctt ctggacggcg
     3241 gctagcagct ggcccatgct gaacggccgg cgcagctcgc cgtccaccgg gacgagcccc
     3301 agcttcccgg tctcgccctg cccggccatg aagatgatca ccttgagcgc cgcgagctcg
     3361 gggtcctccc ggatccgccg gagcgtctcc cagccgtcgc cctctggcat ctccgcgtcg
     3421 agcagcagca gatcgaaccg aacctcgcgc agccggcgga ggccctcgtc ctcgctgtgc
     3481 gcctcctcga cgtcgaaccc ctcgacctgc agccgcagca ccggctgacg gaggtgttcg
     3541 ggccggttgt cgatgatcag gatgcgtttg gcgctcacgg caagggcctt cccttccccc
     3601 tcggccgcca caccctagcg cagcccaccg acgggaacgg ccccggcgcg gcgggtttcg
     3661 gcgccgggga ggttgacgag ggccggtgcg caccgtagcc tctgggcccg ggctgcctcg
     3721 tgcagcccgc ttccgtatgc gacgacgaac gcctttgcga ctcttcgccg tggcggccgt
     3781 ctcggccgcg cttctcgcga ccctggcccc gggcggccac gccgatccgt cgaagcgcga
     3841 cctcgacgcc gctcgcgccg agctcgatgg cctgaacgag cggctctcgc tgctcgtcga
     3901 ggagtacgac gtcgcgaaga tcgaggtcga gcgcctcgag acgcgcctgg ccgacctgcg
     3961 ggagaacgcc tcccgcgccc gggccgccta ccgcgcggcg cgccaggcgc tcgccgagcg
     4021 cgcggccgcc gcctacatcc agggtcccgg ccagggcctg gacgtcctgt tcggctcgac
     4081 gagcttcggc gacttcaccg cgcggctcga gttcctgaac cggatgagcc agcgagacag
     4141 cgacctcgcc gcccgcgccg agcagcgccg ccagcgcctg cgcttcgccg aggcggagct
     4201 gcggccggcg ctcgcgtcgg cgaacgccgc ggtcgccgac ctcgacggga agcaggcaca
     4261 gatcgaggcc gggatcgccg accagcaggc gctggtggcc aagatcgagg cggtcctgca
     4321 acgcaagctc gaggcgcgcc cgaagccgcc ggcgaacccg gccgccccga ccaccgaccc
     4381 cgggccggcg ccgggcccgc tcccgacctc cggtggagcc gccacggcga tcgccgcggc
     4441 gcgctcgatg atcggca
//
LOCUS       DASPWO010000598         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_253967, whole genome shotgun sequence.
ACCESSION   DASPWO010000598 DASPWO010000000
VERSION     DASPWO010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253967"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VEO00_13595"
     CDS             <1..1151
                     /locus_tag="VEO00_13595"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HYU59064.1"
                     /translation="LFGTGTAFVAGVVLGSITETLEVLPGLIILIPAAIGMRGTIFGA
                     IGARLGTSIHAGLFEQTAARSGVLYQNVFVGITLTFSSTLYLAALARLSAAAFGLRSI
                     SFLDFVTISVVGGLLDSAIILLITVGLARLSYGRGYDLDAVSTPVITALADMVTIPTL
                     FLATFLLRVEWLTTAIAVLAILACLYATLRGALTDLPYARRILVEMVAVVLLTPILDV
                     LAGTVVEARLDRFVAFPGLLVLVPPFVAVAGALGGILSSRLSSKLQLGVISPRGRPES
                     PAVLDAAIVTAFGVVAFVLMGALGLGFSVLAGKAHPGAGAMVGGTLIAGLLATGIAIV
                     VSYYVAIVTTRFGLDPDNHSVPIITSVMDLSGVLSFLFVLSVFGVTNG"
     gene            1144..2340
                     /locus_tag="VEO00_13600"
     CDS             1144..2340
                     /locus_tag="VEO00_13600"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HYU59065.1"
                     /translation="MDERPKNVKELLVEAKDTSELMVDLAYAAVFFNEDDLAEEVGRL
                     EEVMDELLRRLREISMLAARSPEDAEAMSGVLHIAAAMEKIGDAASDIARVVQAHLGI
                     PDALRPDLRHADEVVGRVKVREGAPCVGQSLRELLLPTETGMWLIAVRRGAEWEFDPG
                     PETVLTEGDVLLFQGPEEGVNLIRAVAGAPILPEAPESEGPPLTELDRAVDILVEMKN
                     SAEVAVGLAYSSLLFDDQALAAEVATLEARSDILHDELESWVLKAAPEARNSDELRGL
                     LRLAYAGETIFDAARDMTWLVEQGEELHPVVRMALEETEEVAVESVVEGGARAVGHSL
                     KELSVETETGMFVLAVQRGRRWVYRPRPRFVLEAGDRVISIGPEDGIGELQALVGAPA
                     HTAPSA"
BASE COUNT          321 a          859 c          887 g          406 t
ORIGIN      
        1 tcctgttcgg caccgggacg gccttcgtcg ccggcgtcgt cctcggctcg atcaccgaga
       61 ccctcgaggt cctccccggc ctgatcatcc tgatccccgc ggccatcggc atgcggggaa
      121 cgatcttcgg cgcgatcggc gcacggctcg gcacgagcat ccacgccggc ctgttcgagc
      181 agaccgccgc gcgctccggc gtcctctacc agaacgtctt cgtcgggatc acgctgacgt
      241 tctcctcgac gctctacctg gccgcgctgg cacgtctctc ggcggcggcc ttcgggttgc
      301 gctccatctc gttcctcgac ttcgtcacga tctcggtggt cggtggcctg ctcgactcgg
      361 cgatcatcct gctgatcacc gtgggactgg cccggctgtc ctacggacgg ggctacgacc
      421 tggacgccgt gtccacgccg gtgatcacgg ccctggccga catggtgacg atccccacgc
      481 tcttcctggc cacgttcctg ctgcgcgtgg agtggctgac caccgcgatc gcggtgctcg
      541 ccatcctggc ctgcctgtac gccacgctcc ggggcgcgct gaccgacctg ccgtacgcgc
      601 ggcgcatcct ggtcgagatg gtcgccgtgg tgctgctgac cccgatcctc gacgtcctgg
      661 cgggcacggt ggtcgaggct cgtctcgacc ggttcgtggc cttccccggc ctgctggtgc
      721 tcgtcccgcc gttcgtcgcc gtcgccggcg ccctgggcgg catcctctcc tcccgcctgt
      781 cgtcgaagct ccagctcggc gtgatctcgc cgcgcggccg gccggagagc ccggccgtcc
      841 tggacgccgc catcgtcacg gcgttcgggg tggtggcctt cgtgctgatg ggcgccctcg
      901 ggctcggctt cagcgtcctg gcgggcaagg cccacccggg cgccggggcg atggtgggcg
      961 ggacgctcat cgccggtctg ctcgccaccg ggatcgccat cgtggtcagc tactacgtgg
     1021 ccatcgtcac cacccggttc gggctcgatc ccgacaacca ctcggtcccg atcatcacga
     1081 gcgtgatgga cctgtccggg gttctctcct tcctgttcgt tctgtcagta tttggagtga
     1141 ccaatggatg agcgcccgaa gaacgtcaag gagctgctcg tcgaggcgaa ggacacctcc
     1201 gagctgatgg tcgacctggc ctacgcggcc gtcttcttca acgaggacga cctggcggag
     1261 gaggtcggcc ggctcgagga ggtgatggac gagctgctgc ggcgcctccg ggagatctcg
     1321 atgctcgccg ctcgcagccc ggaggatgcc gaggcgatga gcggcgtgct gcacatcgcc
     1381 gcggcgatgg agaagatcgg cgacgccgcg agcgacatcg cccgcgtggt ccaggcgcac
     1441 ctcgggatcc ccgacgcgct gcgtcccgac ctgcgccacg ccgacgaggt ggtcgggcga
     1501 gtcaaggtgc gcgagggcgc gccgtgcgtc ggccagagcc tccgcgagct gttgctcccc
     1561 acggagaccg gtatgtggct gatcgcggtc cgccgcggcg cggagtggga gttcgacccc
     1621 ggcccggaaa ccgtgctcac cgagggcgac gtgctgctgt tccagggtcc cgaggagggc
     1681 gtcaacctga tccgcgccgt cgccggcgcg ccgatcctgc cggaggcgcc cgagtcggag
     1741 ggcccgccac tcacagagct cgaccgcgcc gtggacatcc tggtcgagat gaagaactcc
     1801 gccgaggtcg cggtcggact ggcgtactcg tccttgctgt tcgacgacca ggccctggcg
     1861 gcggaggtcg cgaccttgga ggcgcgcagc gacatcctgc acgacgagct ggagtcctgg
     1921 gtgctgaagg ccgcgccgga agcgcgcaac tccgacgagc ttcggggtct gctgcggctg
     1981 gcgtacgccg gcgagacgat cttcgacgcc gcccgcgaca tgacctggct cgtcgagcag
     2041 ggggaggagc tccacccggt ggtgcgcatg gcgctcgagg agaccgagga agtggcggtg
     2101 gagtcggtcg tcgagggcgg cgcgcgcgcc gtgggccact cgctgaagga gctgtcggtc
     2161 gagaccgaga cggggatgtt cgtgctggcc gtccagcgcg ggcggcggtg ggtgtaccgg
     2221 ccgcggccgc gcttcgtgct cgaggccggg gaccgagtca tctcgatcgg gcccgaggac
     2281 ggcatcggcg agctgcaggc gctggtcggc gccccggcgc atacggcacc ttccgcgtag
     2341 aggggcgaga ggcgtagcct tcaccccatc cgagaagggg gcgcagatgg acgcgtacgt
     2401 ctacgtagag accgagccgg gaaccgcacg acgcgtggcc gaggacatcg cgcgcctcga
     2461 cgtgcgcgag gtc
//
LOCUS       DASPWO010000599        10734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_932902, whole genome shotgun sequence.
ACCESSION   DASPWO010000599 DASPWO010000000
VERSION     DASPWO010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10734
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932902"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VEO00_13605"
     CDS             complement(<1..234)
                     /locus_tag="VEO00_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59066.1"
                     /translation="MRTLGLVALLVIAGCVAPARSFEAYEGKAANAAEAAVSATRTAG
                     LAAQAATRGDAFGPYVSVVMAEAEDAASGAESAF"
     gene            complement(430..1884)
                     /locus_tag="VEO00_13610"
     CDS             complement(430..1884)
                     /locus_tag="VEO00_13610"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HYU59067.1"
                     /translation="MPAIDYHAILPELILSGTILLALVAATALPRQRSWVAMPLSFLG
                     VVGALVATLTLIGSDRATFGGTYVVDNFAVLFKVFFMVVALVVLAVSLRYFREGRFYQ
                     GEYYFLLLSSFLGMLTMPSSRDLLMLFISLELVSAPGFLMAGLRKGDPRSNEAALKFF
                     LIGVLSTAVMLYGMSLIYGVTGTLSLTGIAEVLSGPAGGGNLALAAILFVVAGFAFKV
                     SAAPFQFWAPDTYEGSPIPVAAYLSVASKAAGFAGLLQLMFVAFAPRADFWAPIFAVV
                     SVATMSLGNLVALQQRQVVRLFAYSSIAQSGYMLLPFALVGSDAAINRAAFAAVVLYI
                     LIYAVMNLGAFGVILAVAREAPGALVTDFAGLIRRAPALAVSMVLFLVSLAGIPFLAG
                     FWGKFFIFSAAIDARQAWLAVVMVVNSVISVAYYLSIARQMIFVEAPQDARPLRVPAL
                     VSTVVVLAAAAIVTVGVFPDLFAHFPQVSTLSAP"
     gene            complement(1904..3448)
                     /locus_tag="VEO00_13615"
     CDS             complement(1904..3448)
                     /locus_tag="VEO00_13615"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HYU59068.1"
                     /translation="MDWFDKWAITLAVFIPTVGAAVIAIVPRERDRLARGLGLLFSGV
                     PLLVGILMLFRFDFGMGGAFGRAGALQFEVNLRWIDALNANYHVGLDGISLPLLELTL
                     LLTFLCFIYTWRFLPEPGRPKAFAALLLLLETGMAGTFVAFDLVLFFVFWELVLVPMY
                     FLIGVWGGPNREYAAVKFFLYTIFGSVFMLLGFLAMYFRSDIPGVGHTFDIVLLQQAG
                     FPTGIQNLVFVGLFLGFAIKVPMWPFHTWLPDAHTEAPTVGSVLLAGILLKMGTYGFV
                     RISLPLLPDASRSWAPAIGVLAVIAIVYAALACLAQRDLKRLIAFSSVGHMGFVMLGI
                     ATLTPIGINAAVFGMVAHGVITGMLFFLAGSIHERYHTREIAEIGGGMLSKVPYLGSV
                     LTFTAIASLGLPGLAGFWGEVMALLSSYSPADGLSLGLFRTLMVFGGVGTILTAGYFL
                     WMLQRVNLGTVPGRWQAKVFPDVLAIEWLSWAPLLVGTLLLGVFPRLVFGVTNEAVSN
                     LVGLFT"
     gene            complement(3473..5407)
                     /gene="nuoL"
                     /locus_tag="VEO00_13620"
     CDS             complement(3473..5407)
                     /gene="nuoL"
                     /locus_tag="VEO00_13620"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HYU59069.1"
                     /translation="MIRGAWLIPLLPLAAFFLILFFGKRSPRHGAPIGIAAVAVSWVL
                     SIGVLLQIAQDHPAVERSIEWFNIGSFRLELGMAVDGLAAVMFFVVTTVSLCVQVYSL
                     GYMHGDSRFTWFYAQLSLFTAAMLNVVIANNLFQLLVGWEVMGLCSYFLIGHWWEDKV
                     NSDAGIKAFITTRTGDIPFLFGIFVLTVAAGFDTNIGHIADLAEHGQISTVLLTAGAL
                     LLFGGAIGKSAQFPLHVWLPDAMAGPTPVSALIHAATMVAAGVYLVGRMFAVFVHASA
                     GVLQFVAVIASITMLIAALLAMIQDDIKRVLAYSTVSQLDYMMVGLSLGEAGYTAGLF
                     HLFTHAFFKALLFLAAGSVIHAVHSNYMSEMGGLRSKMPVTFWTFVIGAAALSNIPPF
                     AGFWSKDEIISTAFHSGHYFVWAVAFVTAIVTAFYMTRAVLLTFFGDYRGTAHPHEAP
                     VSITAPLVLLAFLSVTAGFLNATAFNLPAPFTAWVHFGAAEHEPFNYGFAAVSILGAA
                     GGLAVGYRLYRGWRERDPVRNLGPVYTVLERKYYLDDIYMGGVVRPIQYRLSAAANWF
                     NQRVLDGIVDGAAVVTRWFASLVNLFDQKAVDGAVNGAAGLTGFTGGILRYLQSGNVQ
                     RYAVFLFAGVVALAVIFTRL"
     gene            complement(5412..5717)
                     /gene="nuoK"
                     /locus_tag="VEO00_13625"
     CDS             complement(5412..5717)
                     /gene="nuoK"
                     /locus_tag="VEO00_13625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HYU59070.1"
                     /translation="MRLWLPLTFSAILFAAGIYGVLARRNAVLVLMSIELMLNAVNVN
                     LVAFSQYLKDALQAGQIFTLFVITVAAAEVGIGLAIVILIFRNRETVNVDEVNLLKW"
     gene            complement(5717..6211)
                     /locus_tag="VEO00_13630"
     CDS             complement(5717..6211)
                     /locus_tag="VEO00_13630"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HYU59071.1"
                     /translation="MTAQEIVFVVVAAVGTVGAFRLVTSKNVVHAALYLVVALGMVGA
                     VYLLLAAEFVAWVQILIYVGAIVVLLLFSLMLTKAPIGREALDNQQRGVAALVALAVF
                     AGLLLLIRDTFPDQRIEVQATNTADIGNSLFRGFVLPFEVVSILLLAALVGAVVIARR
                     DGSE"
     gene            complement(6216..6953)
                     /locus_tag="VEO00_13635"
     CDS             complement(6216..6953)
                     /locus_tag="VEO00_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003998883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HYU59072.1"
                     /translation="MLKRSVTQQYPHVRPDLPPRTRGVIALKQENCTVCYKCSRACPD
                     WCIYIDAHKETHEPAGGGRGRSYKVLDRFDIDYALCMYCGICVEVCPFDALFWTPEFE
                     YAEYRISDLLHDKERLQDWTYTVLPPPPLEDGAEEPEPAPAAAQAPPAPAPAAPAPAA
                     RAPAAPAEAAPAPAAPPPAAEAAPAPAAEAPAAAERARHVEVQPDQETYDRVLQGEQA
                     KGTDARVAEGRAKAAAIRAARKKAQGG"
     gene            complement(7083..8069)
                     /gene="nuoH"
                     /locus_tag="VEO00_13640"
     CDS             complement(7083..8069)
                     /gene="nuoH"
                     /locus_tag="VEO00_13640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HYU59073.1"
                     /translation="MSELNQLFSNFWIVLLLKVAAVMIVFLTAPLGVGYMEHKVLAHM
                     QARLGPMEAGRFHGWAQLIADGVKFIQKEDVIPRAADRWVFSLAPAVVLVPTIAVLLV
                     LPFGPSYFAENFDVGLFFVMAISAVSVIGVLMAGWASANKFSLIGALRAAAQLIAYEL
                     PLILSAVSVAMIAGSLSLVKISEAQHDYWYALVQPVGFLIFLMASLAELTRPPFDMPI
                     ADSEIIFGAYTEYTGMKFAFFLLSEYAGIVVLSGIATVLFLGGYQKVPFLPIPGWIVM
                     QGKIFGLAFFIIWLRATYPRLREDQLQRFSWIVLIPLSLANILVTGLVKVLV"
     gene            complement(8066..8584)
                     /locus_tag="VEO00_13645"
     CDS             complement(8066..8584)
                     /locus_tag="VEO00_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYU59074.1"
                     /translation="MASSPSRSRLAPAEVAERLRAQFGDDLVDVRESFGHTQVTVRPE
                     RYRELVLLVRGDPDLRCDFLDFVAGVDYRDRGFEVVTQVTSTSTGVSVRIKVPCEAEH
                     PVCPTLSDVYPGADWHERETWELTGIVFEGHPHLVKLVLPEQFEGFPLRKSFELMSRV
                     AKPWPGDQELED"
     gene            complement(8544..9047)
                     /locus_tag="VEO00_13650"
     CDS             complement(8544..9047)
                     /locus_tag="VEO00_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006382283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HYU59075.1"
                     /translation="MERVPMPQPVKFLLNWGRRYSLWVMNFGLACCAIEFIAASTSKH
                     DFIRFGVIPFAHGPRQADLLVVAGTLTDKMAPALKRVYDQMPDPKYVISFGSCSNCGG
                     PYWDAYSVTKGVDQAVPVDVYVPGCPPRPEALLHGILKLQEKIAHEDIREKWSGKLTQ
                     PQPARAR"
     gene            complement(9047..9412)
                     /locus_tag="VEO00_13655"
     CDS             complement(9047..9412)
                     /locus_tag="VEO00_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYU59076.1"
                     /translation="MSGYYQSYGTLIALAVAGAGLVAVAILFARIVAPRRLVPEKLTT
                     YECGIDPVGEGWSQSQIRYYIYAFLFVIFDVESVFLFPWAVVFERLGMFALVEMTIFI
                     VILALGLVYAWRKGVLRWV"
     gene            complement(9544..10539)
                     /locus_tag="VEO00_13660"
     CDS             complement(9544..10539)
                     /locus_tag="VEO00_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59077.1"
                     /translation="MKERRIPAPRVALLVALALAAAACSSGGGSAETSPSVSGPGGEF
                     SAQVASFDLSADRPQRLMVGILTSENAFVAFGRVTFRFAYLGTRENPVDPPEAVGTPV
                     EATFLPLVGTNPADVADGPRPIRSSEGPGVYEIRDHRFDRAGFWQVEVAADVRGLGPQ
                     RATAAFEVLEHPAVPAPGDEALPTENHTIGEKGIAPEAIDSRAATGTIPDPELHRYTI
                     KGAIAASLPAVVVFSTPVYCVSRFCGPVTDAVQALAARYGDRAVFIHVEIWRDFQGQT
                     VNEAAADWLLRNDNLNEPWVFLIGADGVIVDRWDNLLDEDELEAQLRDLPPGAVT"
BASE COUNT         1952 a         3677 c         3750 g         1355 t
ORIGIN      
        1 gaaggcgctc tcggcgccgg acgcggcgtc ctccgcctcg gccatgacca ccgagacgta
       61 gggcccgaaa gcgtctcccc gcgtggccgc ctgcgcggcg aggccggcgg tgcgcgtcgc
      121 cgacaccgcg gcctctgccg cgttggcggc cttgccctcg taggcctcga aggacctggc
      181 cggcgccacg caccccgcga tcacgaggag cgcaaccaga ccgagcgttc gcatcgggaa
      241 ggtggttccc gcggccggtg cgggcaaacc cgtgtgaccg cgcggcgcgc tgggtacaca
      301 ccgcaccgcg aaagaggttc ccgtgggtgc aagggcagtg cggagtcgaa ggacggcgac
      361 gacgagatgc gcgccgcgct cgaccggatc gaggagaagc tcgggaccaa accctggtag
      421 gtgcccgggc tacggggccg acagggtcga cacctggggg aagtgcgcga acaggtcggg
      481 gaacactccg accgtcacga tcgccgccgc ggccaggacg acgacggtcg agacgagcgc
      541 cgggacgcgc agggggcggg catcctgcgg agcctccacg aagatcatct gccgggcgat
      601 cgacaggtag tacgccaccg agatcacgga gttcaccacc atgaccacgg ccagccaggc
      661 ctggcgggcg tcgatcgccg cgctgaagat gaagaacttc ccccagaacc cggccaggaa
      721 cgggatgccc gccagcgaca ccaggaacag caccatcgac acggcgagcg ccggcgcgcg
      781 gcggatcagg ccggcgaagt cggtgaccag ggcgcccggc gcttcccgtg ccaccgccag
      841 gatcacgccg aaggccccga ggttcatcac cgcgtagatc aggatgtaca gcacgacggc
      901 ggcgaaggcc gcccggttga tggcggcgtc ggacccgacg agcgcgaacg gcaggagcat
      961 gtacccggat tgggcgatgc tcgagtacgc gaacagccgc accacctggc gctgctgcag
     1021 cgcgacgagg ttgccgagcg acatcgtcgc caccgagacc accgcgaaga tcggcgccca
     1081 gaagtcggcc cggggggcga aggccacgaa catcagctgc agcaggccgg cgaacccggc
     1141 cgccttcgac gccacagaca ggtaggcggc caccgggatc ggcgatccct cgtacgtgtc
     1201 cggcgcccag aactggaacg gagcggccga taccttgaag gcgaagccgg ccaccacgaa
     1261 caggatcgcc gcgagggcca ggttcccgcc gcccgccggg ccggacagga cctcggcgat
     1321 gccggtcagg ctgagggttc cggtgacccc gtagatcagg ctcatgccgt agagcatcac
     1381 cgcggtggag agcaccccga tcaggaagaa cttcagcgcg gcctcgttcg agcgcgggtc
     1441 gcccttgcgc agcccggcca tcaggaaccc cggggccgag acgagctcga gcgagatgaa
     1501 cagcatcagc aggtcgcggg acgacggcat cgtcagcatc ccgaggaacg acgagagcag
     1561 caggaagtag tactcgccct ggtagaagcg gccctcccgg aagtagcgca gcgaaacggc
     1621 caggacgacg agcgccacga ccatgaagaa caccttgaac agcacggcga agttgtcgac
     1681 gacatacgtc ccgccgaacg tcgcccggtc ggagccgatc agggtcagcg tcgccacgag
     1741 ggccccgacc actccgagga acgacagcgg catcgccacc caggagcgct ggcgcggcag
     1801 cgccgtggcc gccaccaggg ccagcaggat cgtgccggag aggatcagct ccggcaggat
     1861 ggcgtggtag tcgatcgccg gcatcagcgt cttctgagac cggtcacgtg aacaggccga
     1921 ccaggttcga gacggcctcg ttcgtcacgc cgaacaccag gcgagggaag acgccgagca
     1981 gcagcgtccc gacgagcagc ggtgcccacg acagccactc gatggcgagc acgtctggga
     2041 agaccttggc ctgccagcgg ccgggcaccg tccccaggtt gacccgctgc agcatccaca
     2101 ggaagtagcc ggcggtcagg atcgtaccga cgccaccgaa gaccatcagc gtgcggaaca
     2161 ggccgagcga caggccgtcg gccgggctgt aggaggagag cagggccatc acctcgcccc
     2221 agaagccggc cagcccgggc aggccgaggg acgcgatcgc cgtaaacgtc agcaccgagc
     2281 cgaggtacgg caccttcgag agcatcccgc cgccgatctc ggcgatctcg cgggtgtggt
     2341 accgctcgtg gatcgaaccg gccaggaaga acagcatccc ggtgatcacg ccgtgggcga
     2401 ccatcccgaa gacggccgcg ttgatcccga tcggcgtcag ggtggcgatg ccgagcatga
     2461 cgaaccccat gtggccgacg gaggagaacg cgatcagccg cttcaggtcg cgttgcgcca
     2521 ggcaggccag cgcggcgtag acgatcgcga tcaccgccag gacgccgatc gccggggccc
     2581 agctccgcga ggcgtccggg agcagcggca gcgagatgcg gacgaagccg taggtcccca
     2641 tcttcagcag gatgccggcc agcaggaccg agccgaccgt cggggcctcg gtgtgcgcgt
     2701 cgggaagcca ggtgtggaag ggccacatgg gcaccttgat cgcgaacccg aggaacaggc
     2761 cgacgaagac caggttctgg atccccgtcg ggaacccggc ctgttgcagc aggacgatgt
     2821 cgaacgtgtg cccgacgccg gggatgtccg agcggaagta catcgccagg aaccccagca
     2881 gcatgaacac ggacccgaag atcgtgtaca ggaagaactt caccgccgcg tactcgcggt
     2941 tcgggcctcc ccacaccccg atcaggaagt acatcgggac gagcaccagc tcccagaaga
     3001 cgaagaacag caccaggtcg aaggccacga acgtcccggc catgcctgtc tccaggagta
     3061 gcagcagcgc ggcgaacgcc ttcggccgcc ccggctcggg gaggaaccgc cacgtgtaga
     3121 tgaagcagag gaacgtcagc agcagggtca gctcgagcag cggcaggctg atcccgtcga
     3181 ggccgacgtg gtagttggcg ttcagcgcgt cgatccagcg caggttgacc tcgaactgca
     3241 gggcgcccgc ccggccgaac gcgccgccca tcccgaagtc gaagcggaag agcatcagga
     3301 tcccgaccag gagcgggacg ccggagaaca ggagccccag accgcgcgcc aggcgatcgc
     3361 gttccctcgg cacgatcgcg atcacggcgg cgccgacggt cgggatgaac accgccagcg
     3421 tgatcgccca cttgtcgaac cagtccatca gcctctcctc aggtcccccc ggtcagagcc
     3481 gcgtgaagat caccgccagc gcgacgacgc cggcgaacag gaacaccgcg tagcgctgca
     3541 cgttgcccga ctgcaggtag cgcaggatgc cgccggtgaa cccggtcagc ccggccgcgc
     3601 cgttcacggc gccgtccacc gccttctggt cgaacaggtt caccaggctg gcgaaccagc
     3661 gcgtgaccac cgcggcgccg tccacgatgc cgtcgaggac ccgctggttg aaccagttcg
     3721 ccgcggccga cagccggtac tggatcggcc ggacgacccc acccatgtag atgtcgtcca
     3781 ggtagtactt ccgctcgagc acggtgtaga cgggaccgag gttgcgaacc gggtcgcgtt
     3841 cgcgccagcc gcggtagagg cggtagccga cggccagccc gccggccgcg ccgaggatcg
     3901 agaccgcggc gaacccgtag ttgaacggct cgtgctcggc ggccccgaag tggacccacg
     3961 cggtgaacgg cgcgggaagg ttgaaggcgg tggcgttcag gaagccggcg gtcaccgaca
     4021 ggaacgccag cagcacgagc ggcgcggtga tcgacaccgg cgcctcgtgt gggtgcgcgg
     4081 tcccccggta gtcgccgaag aaggtcagca gcaccgcccg cgtcatgtag aaggcggtca
     4141 cgatcgcggt gacgaacgcc accgcccaga cgaaatagtg cccggagtgg aaggcggtcg
     4201 agatgatctc gtccttcgac cagaagccgg cgaacggcgg gatgttggac agggccgccg
     4261 ccccgatgac gaacgtccag aacgtgaccg gcatcttcga tcgcaggccg cccatctcgc
     4321 tcatgtagtt ggagtgcacg gcatggatca ccgagccggc ggcgaggaac agcagggcct
     4381 tgaagaacgc gtgggtgaac aggtggaaca gtccggcggt gtaccccgcc tcccccagcg
     4441 agagcccgac catcatgtag tcgagctgcg ataccgtcga gtacgccagt acccgtttga
     4501 tgtcgtcctg gatcatcgcc agcagggccg cgatcagcat ggtgatcgag gcgatcaccg
     4561 ccacgaactg cagcacgccc gccgaggcgt gcacgaacac cgcgaacatc cgcccgacca
     4621 gatagacccc ggcggccacc atcgtcgcgg cgtggatcag cgccgatacg ggcgtcgggc
     4681 cggccatcgc gtcgggcagc cagacgtgca gcgggaactg ggccgacttg ccgatcgccc
     4741 cgccgaacag gaggagcgcg ccggccgtga gcagcaccgt cgagatctgg ccgtgctcgg
     4801 ccaggtcggc gatgtggccg atgttcgtgt cgaacccggc ggccacggtc aggacgaaga
     4861 tgccgaacag gaacgggatg tcaccggtgc gcgtggtgat gaacgccttg atcccggcgt
     4921 cggagttgac cttgtcttcc caccagtggc cgatcaggaa gtaggagcag aggcccatca
     4981 cctcccaccc gaccagcagc tggaagaggt tgttggcgat gaccacgttg agcatcgccg
     5041 cggtgaagag ggagagctgg gcgtagaacc aggtgaaccg ggagtcgccg tgcatgtagc
     5101 cgagcgagta cacctgcacg cacagcgaga ccgtcgtcac gacgaagaac atcaccgccg
     5161 cgagcccgtc gacggccatg ccgagctcca ggcggaacga gccgatgttg aaccactcga
     5221 tcgagcgctc gacggccgga tggtcctgcg cgatctgcag caggacgccg atcgagagca
     5281 cccaggacac ggcgaccgcg gcgatcccga tcggcgcgcc gtgccgtggc gagcgcttgc
     5341 cgaagaacag gatcaggaag aacgccgcca gcggcagcag cgggatcagc caggcgcccc
     5401 ggatcacgcg gtcaccactt gaggaggttc acctcgtcga cgttgaccgt ctcccggttc
     5461 cggaagatca ggatgacgat cgccagcccg atgcccacct ccgcggcagc cacggtgatc
     5521 acgaacaggg tgaagatctg acccgcctgc agcgcgtcct tcaggtactg cgagaaggcc
     5581 accaggttca cgttgaccgc gttgagcatc agctcgatcg acatcaggac gagcaccgcg
     5641 ttgcgccgtg cgagcacgcc gtagatgccg gcggcgaaca ggattgccga gaacgtcagc
     5701 ggcagccaga ggcggatcac tcggagccgt cccggcgggc gatgaccacg gccccgacca
     5761 gggccgcgag cagcaggatc gagaccacct cgaacggcag gacgaagccg cggaacaggg
     5821 agttcccgat gtccgcggtg ttcgtcgcct ggacctcgat ccgctggtcc gggaaggtgt
     5881 cgcggatcag caggagcagc ccggcgaaca cggccagggc cacgagggcg gccacgcccc
     5941 gctgctggtt gtcgagcgcc tcccgcccga tcggcgcctt ggtcagcatc aggctgaaca
     6001 ggagcaggac cacgatcgcg ccgacgtaga tcaggatctg cacccaggcc acgaactccg
     6061 cggcgagcag caggtagacg gcaccgacca tcccgagggc cacgaccagg tacagggccg
     6121 cgtggaccac gttcttcgag gtgaccagcc ggaacgcgcc gaccgtcccg accgcggcga
     6181 ccaccacgaa gacgatctcc tgagcggtca acgcctcagc cgccctgggc cttcttccgg
     6241 gcggcgcgga tcgcggccgc cttcgcccgg ccttcggcca cgcgcgcgtc cgttcccttc
     6301 gcctgctccc cctgcaggac ccggtcgtag gtctcctggt cgggctgaac ctcgacgtgc
     6361 cgcgcccgct cggccgccgc cggagcctcg gcggcgggag ctggagcggc ctcggcggca
     6421 ggaggcggcg ctgccggagc cggagccgcc tcagcgggag cggcgggggc gcgagccgcg
     6481 ggagcgggag ctgccggggc cggggccggc ggcgcctggg cggcggccgg ggcgggctcg
     6541 ggctcctcgg cgccgtcctc gagcggtggc ggagggagca ccgtgtaggt ccagtcctgc
     6601 agccgctcct tgtcgtgcag caggtcgctg atccggtact cggcgtactc gaactccggc
     6661 gtccagaaca gcgcgtcgaa cggacagacc tcgacgcaga tgccgcagta catgcagagc
     6721 gcgtagtcga tgtcgaagcg gtccaggacc ttgtacgagc ggccgcggcc gccgccggcc
     6781 ggctcgtggg tctccttgtg cgcgtcgatg tagatgcacc agtccgggca cgcgcgcgag
     6841 cacttgtagc agaccgtgca gttctcctgc ttgagcgcga tgaccccccg ggtgcgcggc
     6901 ggcaggtccg ggcggacgtg cgggtattgc tgggtcaccg agcgcttcag catcgttcgg
     6961 agcgtcacca gcaggccctt gatcaggccg agcccgggga cgggcggccg gaggcgcttc
     7021 tcggggatgc ggcccctcgg agcctctggg gcagcctccg cgcgccgctc ctccgtctgc
     7081 acctagacca gcaccttcac caagccggtg accaggatgt tcgccagcga gagcgggatc
     7141 agcacgatcc aggagaagcg ctgcagctgg tcctcgcgca ggcggggata ggtggcccgc
     7201 agccagatga tgaagaaggc cagcccgaag atcttcccct gcatcacgat ccatcccggg
     7261 atcgggagga acgggacctt ctggtagccc ccgaggaaca ggaccgtggc gatcccggag
     7321 agcaccacga tgccggcgta ctccgacagc aggaagaatg cgaacttcat gccggtgtac
     7381 tccgtgtagg cgccgaagat gatctcggag tccgcgatcg gcatgtcgaa cggcggccgg
     7441 gtcagctcgg ccagcgaggc catcaggaag atcaggaacc ccaccggctg gacgagcgcg
     7501 taccagtagt cgtgctgggc ctccgagatc ttcaccagcg acagcgagcc ggcgatcatc
     7561 gccacggaca ctgccgacag gatcagcggc agctcgtagg cgatcagctg cgccgcggcg
     7621 cggagcgcgc cgatcaggga gaacttgttc gccgaggccc agccggccat cagcaccccg
     7681 atcaccgaga ccgccgagat ggccatgacg aagaacaggc cgacatcgaa gttctccgcg
     7741 aagtaggacg gcccgaacgg cagcaccagc agcaccgcga tcgtcggcac gagcacgacg
     7801 gccggcgcga gggagaacac ccaccggtcg gcggcgcgcg ggatgacgtc ctccttctgg
     7861 atgaacttca cgccgtcggc gatcagctgc gcccagccgt ggaaccggcc ggcctccatc
     7921 gggccgagcc gggcctgcat gtgcgccagg accttgtgct ccatgtaccc gacgccgagc
     7981 ggcgcggtga ggaacacgat catcaccgcc gccaccttca gcagcagcac gatccagaag
     8041 ttggagaaca gctggttgag ctcgctcaat cctcgagctc ctggtccccg ggccacggct
     8101 tggcgacgcg gctcatcagc tcgaacgact tgcgcagcgg gaatccctcg aactgctccg
     8161 ggaggaccag cttcaccaga tggggatggc cctcgaagac gatgccggtc agctcccagg
     8221 tctcccgctc gtgccagtcg gcgcccgggt agacgtccga gagcgtggga cagaccggat
     8281 gctcggcctc gcaggggacc ttgatccgca cggagacacc ggtgctggtg gaggtcacct
     8341 gcgtcacgac ctcgaagccc cggtcgcggt agtccacgcc ggccacgaag tcgaggaagt
     8401 cgcagcgcag gtcgggatcg ccccggacga ggagcaccag ctcgcggtag cgctccgggc
     8461 gcaccgtcac ctgggtgtgc ccgaagctct cccggacgtc cacgaggtcg tcgccgaact
     8521 gcgcccggag gcgctcggcg acctcagcgg gcgcgagccg gctgcggctg ggtgagcttg
     8581 ccactccact tctcccggat gtcctcgtgg gcgatcttct cctgcagctt gaggatgccg
     8641 tgcagcaggg cctcgggccg cggtgggcag ccggggacgt agacgtcgac cgggacggcc
     8701 tggtccacgc ccttcgtcac cgagtaggcg tcccagtacg ggccgccgca gttcgagcac
     8761 gacccgaacg agatgacgta cttcgggtcg ggcatctggt cgtagacgcg cttcagcgcc
     8821 ggcgccatct tgtcggtcag cgtgccggcc acgaccagca ggtctgcctg ccgcgggccg
     8881 tgcgcgaacg ggatcacgcc gaaacggatg aagtcgtgct tggaggtgga ggcggcgatg
     8941 aactcgatcg cgcagcaggc caggccgaag ttcatcaccc acagcgagta gcgcctgccc
     9001 cagttcagca ggaacttcac cggctggggc atcggcaccc gttccatcag acccaccgca
     9061 gcaccccctt gcgccaggcg tacaccaggc cgagggccag gatgacgatg aagatggtca
     9121 tctcgacgag ggcgaacatc ccgagacgct cgaagacgac ggcccagggg aacaggaaca
     9181 cgctctcgac gtcgaagatc acgaacagga acgcgtagat gtagtagcgg atctgcgact
     9241 gcgaccagcc ctcgccgacc gggtcgatgc cgcactcgta cgtggtgagc ttctcgggga
     9301 ccagccggcg tggggcgacg atccgggcga acaggatggc cacggccacc aggccggcgc
     9361 ccgccacggc gagcgcgatc agggttccgt aggactggta gtagccgctc aagaacgtcc
     9421 cgtccggtcc gcttgtgaac gctttcccaa gccggattct atcagcgatg cacggagggc
     9481 tccgcccccg tgtacccccc gaagagcaag gggtcgacta ctgcgtgccg ctctccccgc
     9541 cgcttacgtg accgcgccgg gggggagatc tcgaagctgc gcttcgagct cgtcctcgtc
     9601 gagcaggttg tcccagcggt cgacgatcac gccgtcggcc ccgatcagga acacccacgg
     9661 ctcgttcagg ttgtcattcc gcaggagcca gtcggcggcc gcctcgttca cggtctggcc
     9721 ctggaagtcg cgccagatct ccacgtggat gaacaccgcc cggtcgccgt agcgggccgc
     9781 gagggcctgc acggcgtcgg tgaccgggcc gcagaagcgg ctgacgcagt acacgggcgt
     9841 ggagaagacc accaccgcgg ggaggctggc ggcgatcgcc cccttgatcg tgtaccggtg
     9901 cagttccggg tccgggatcg ttcccgtggc ggcccgcgag tcgatcgcct ccggcgcgat
     9961 gcccttctcc ccgatggtgt ggttctcggt tggaagcgcc tcgtcgccgg gcgcggggac
    10021 cgccgggtgc tcgagcacct cgaacgcggc ggtggcgcgc tgggggccga ggccccgcac
    10081 gtcggcggcc acctccacct gccagaagcc ggcccggtcg aaccggtggt cgcggatctc
    10141 gtacaccccg gggccctccg acgagcggat cgggcgcggg ccgtccgcga cgtcggccgg
    10201 gttcgtcccg accaggggga ggaaggtcgc ctcgaccggc gtcccgaccg cctccggcgg
    10261 atcgaccggg ttctcccgcg tcccgaggta ggcgaagcgg aaggtgaccc ggccgaacgc
    10321 gacgaacgcg ttctcgctgg tcaggatgcc gaccatcagc cgctgcgggc ggtcggccga
    10381 caggtcgaag ctcgcgacct gcgcgctgaa ctcgccaccg ggcccggaga cgctgggcga
    10441 cgtctccgcc gagccgcctc cgctcgagca cgctgcggcg gccagcgcga gcgcaacgag
    10501 caacgccacc cgcggcgccg ggatccggcg ttccttcatc cgaggatcat cccgcaccgc
    10561 gctccgtttg gccgggccgg cactcagggg cagcgcggcg ccgccaggcc gaggcggtcc
    10621 aggctggccc gggtggcctc gatcagcgcg tcgagatcgg cggcggtccg ctcgggctcg
    10681 gcatcctgcc gaacgtcggc ctccacgctc gcgatcgcct ccagcaggcg cgcg
//
LOCUS       DASPWO010000600         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1014468, whole genome shotgun sequence.
ACCESSION   DASPWO010000600 DASPWO010000000
VERSION     DASPWO010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014468"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1129
                     /locus_tag="VEO00_13665"
     CDS             227..1129
                     /locus_tag="VEO00_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYU59078.1"
                     /translation="MIELQDIAYVRSGVADLPAAVRFATDIVGLELVASDEQGVAHLR
                     ADARHHCLALVAGRSGVIASGFTVADDGALQGAERELERRGFAVRRLDAAAARSRRVA
                     AGIAFDDPFGNRLELVTQQECLARPVAFGRASGITEFGHLCLDAPDVHEAYRFWSGAF
                     NAKVSDWIGDAACLMRIDPVHHKLAVFKGDGPGLCHMNFQVASIDDVFRNWHFLVDHG
                     VEIEMGPGRHPQSTAVFLYFLGPESFTYEYSFGVRRIEDDAAWRPRTFDPAEPGSIDM
                     WLGPTSRPTTQPQARDDLPATLSV"
     gene            complement(1126..1902)
                     /locus_tag="VEO00_13670"
     CDS             complement(1126..1902)
                     /locus_tag="VEO00_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYU59079.1"
                     /translation="MARPAAAVPGIERLELPEIRQAIPRLHAYLRECILDGRLPPGTK
                     LSQVALAKQLGVSRTPLREVLRMLQEEGYVEFEPNQRMRVAGLDPVELDADYACRIVL
                     ETLALSMTLESIGSRQRREASRLLTAMRRAARAGDLPGWFRAHHAYHRAMTAGAGEPL
                     LRQLQSLEDRSVRYIRIYQQSEPASWQTAGDAEHAAILDALAAGDDRAALSGLAHHLA
                     RTALRVLSDCAPGYTPRAVPHAVALAEGADPAALRVASAG"
BASE COUNT          273 a          741 c          737 g          288 t
ORIGIN      
        1 gcgagctcac cgtcgaggac ggcgacctca cgcccagcct caagctcaag cgccgggccg
       61 tcaccgccag gcaccgcgac ctgctggaat ccctctacgg cgacccggcg gcggccacca
      121 gccccgccga ccagccgacc cccgcgtagc ggcgcggtac ccgcggcgcg gtcttgtccc
      181 gagccgaaga aactgtgtac agtttctcgg ggaagggagg ttgacggtga tcgagctcca
      241 ggacatcgcc tacgtgcgat ccggcgtcgc ggacctgccg gcagcggtcc ggttcgcgac
      301 cgacatcgtg gggctcgagc tggtggcctc ggacgagcag ggggtcgcgc accttcgggc
      361 cgacgcgcgc caccactgcc tcgccctggt cgcgggccgg tccggggtca tcgcatccgg
      421 cttcaccgtc gccgacgacg gcgccctcca gggcgcggag cgcgagctgg aacgtcgtgg
      481 cttcgcggtc cggcgtctcg acgccgccgc cgcgcggtcg cgccgggtcg ctgccggcat
      541 cgccttcgac gaccccttcg gcaaccggct cgagctggtg acgcagcagg agtgcctcgc
      601 ccggccggtc gccttcgggc gcgcgtcggg gatcacggag ttcggtcacc tctgcctcga
      661 cgcgccggac gtccacgagg cctaccggtt ctggtccggc gccttcaacg ccaaggtgtc
      721 cgactggatc ggcgatgccg cctgcctcat gcggatcgac cccgtgcacc acaagctcgc
      781 cgtcttcaag ggcgacgggc cgggcctgtg ccacatgaac ttccaggtcg cctcgatcga
      841 cgacgtcttc cgcaactggc acttcctggt cgaccacggc gtcgagatcg agatggggcc
      901 gggacggcac ccgcagtcga cggccgtgtt cctctacttc ctgggtcccg agagcttcac
      961 ctacgagtac tccttcgggg tgcgccggat cgaggacgac gcggcgtggc ggccacggac
     1021 cttcgacccc gccgagccgg gatcgatcga catgtggctc gggccgacct cgcgaccgac
     1081 gacccagccg caggcgcgcg acgacctgcc cgcgacgctc tccgtctaac ccgcggacgc
     1141 cacccgcagc gcggcagggt cggcgccctc tgcgagggcg acggcgtgcg ggaccgcccg
     1201 cggcgtgtag ccgggcgcgc agtccgacag cacccgcagc gcggtgcggg cgaggtggtg
     1261 ggcgagcccg gagagggccg cgcggtcgtc cccggcggcg agggcgtcca ggatcgccgc
     1321 gtgctcggcg tcgccggcgg tctgccagct ggccggttcc gactgctggt agatgcggat
     1381 gtagcggacg ctgcggtcct ccagcgactg cagctggcgc agcagcggct cgcccgctcc
     1441 ggcggtcatc gcccggtggt aggcgtggtg cgcgcggaac cacccgggga ggtcgccggc
     1501 ccgcgcggcc cgccgcatgg cggtgagcag ccggctcgcc tcccggcgct gacgcgagcc
     1561 gatggactcc agggtcatcg acagcgccag cgtctcgagc acgatgcggc aggcgtagtc
     1621 ggcgtcgagc tcgaccgggt cgaggccggc cacgcgcatc cgctggttgg gctcgaactc
     1681 gacatagccc tcctcctgga gcatccgcag cacctcgcgc agcggcgtgc ggctcacgcc
     1741 gagctgcttg gcgagcgcca cctgcgacag cttggtgccc ggtgggaggc ggccgtcgag
     1801 gatgcactcg cgcaggtacg cgtgcagtcg gggaatggcc tgccggatct cggggagctc
     1861 gaggcgctcg atcccgggga cggcggctgc cggtcgtgcc atgggcgcat tgtagccctc
     1921 gccgaccgca actgcataca catctctggt cggactcttg acgcggtgga gccaaagtgc
     1981 atacagtttc tcaacgtcgc gaggggaggt gcccgatgcc ggtcgaacgc cccgcgatg
//
LOCUS       DASPWO010000601         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_165533, whole genome shotgun sequence.
ACCESSION   DASPWO010000601 DASPWO010000000
VERSION     DASPWO010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165533"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VEO00_13675"
     CDS             complement(<1..286)
                     /locus_tag="VEO00_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HYU59080.1"
                     /translation="MAKATVEGIFVAPEREAPLQAVDAVEAVTGVGLDGDRYAARAGT
                     YSGKEGPKRQVTLIEAEALEAAERDYGVALPPGSTRRNIVTRGVALNHLVG"
     gene            complement(316..1650)
                     /locus_tag="VEO00_13680"
     CDS             complement(316..1650)
                     /locus_tag="VEO00_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HYU59081.1"
                     /translation="MARAVRGPGWRNWSGSVRFTPRRLARPESIEEVVALVRAARDAG
                     ETLRVAGAGHSFTPLAVTEGTLVSLDGFHGLLAADPAAGQATVRAGTRLRALGDALLG
                     HGAAPENLGDIDAQSIAGATSTGTHGTGAALGGLAAQVTGLTLVTGVGDVVECSEKSD
                     PDLLSAARVSLGALGVIVLVTLRVLPAYRLRYRSERMRLDEVVADLDRHLAEHRHFEF
                     YAFPYSPWVQAKWQDPTEEPARDRRLRAFVNDEVMENAAVWTLCAAARAAPRLSARIG
                     KVMGRAVGPYGGVGHAHRVFATPRRVRFNEMEYALPRERLPDVLQEVQATIERRRLRV
                     HFPIEVRFSAGDDIWLSTAYGRDSAYIAVHVFRGMPYRGYFAAMEEIFVRAGGRPHWG
                     KLHSLGAPELRPRYPRWDDVLAARARLDPDGVFLNPHLRHLFGLPGAPGRAD"
     gene            complement(1656..>2318)
                     /locus_tag="VEO00_13685"
     CDS             complement(1656..>2318)
                     /locus_tag="VEO00_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018754644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HYU59082.1"
                     /translation="SDRLLLDGIMGYEAQVAGLPDRTPGQGLRGPVIRALKRRSVRTV
                     RERRAAIVRAVEAVAGPLRFVNGGGTGSLETTTAEDVVTEAAAGSAFFAPGLFDHYDA
                     FRHLPAAAFAIPVVRRPAPDVYTCLGGGYIASGATGASKQPVPYLPAGARLLTNEEAG
                     EVQTPIRYRGPERLAIGDPVFLRHAKAGELCERFDRLLLATADGMAGEVPTYRGDALT
                     FL"
BASE COUNT          308 a          892 c          840 g          278 t
ORIGIN      
        1 ggccgaccag gtggttcagc gcgacgccgc gcgtgacgat gttccgtcgc gtcgatccgg
       61 gcggcagcgc gaccccgtag tcgcgctccg cggcctcgag cgcctcggcc tcgatcagcg
      121 tcacctggcg cttcggcccc tccttgccgg agtaggtccc ggcgcgcgcc gcgtagcggt
      181 caccgtccag cccgacaccg gtgaccgcct ccacggcgtc cacggcctgc agcggcgcct
      241 cgcgctccgg cgcgacgaag atcccttcca cggtcgcctt cgccatcggc gtcatggtag
      301 tcggcgcgcc ccccgttagt cggcacgacc cggcgcgccg ggcaggccga acaggtggcg
      361 caggtgcgga ttcaggaaga ccccgtcggg atccagccgg gcgcgggcgg ccaggacgtc
      421 gtcccagcgc gggtagcgcg gccgcagctc gggggctccc agcgagtgga gcttccccca
      481 gtgcggccgg ccgccggcgc ggacgaagat ttcctccatg gccgcgaagt acccgcggta
      541 cggcatgccc cggaagacgt gcaccgcgat gtaggcgctg tcgcggccgt acgccgtgga
      601 cagccagatg tcgtcgccgg cggagaagcg gacctcgatc gggaagtgca cgcgcaggcg
      661 gcggcgctcg atcgtcgcct ggacctcctg gagcacgtcc ggcagacgct cgcgcggcag
      721 ggcgtactcc atctcgttga accgcacgcg gcgcggagtg gcgaacaccc tatgcgcgtg
      781 cccgacgccg ccgtacgggc cgacggcgcg tcccatcacc ttcccgatcc tcgcggagag
      841 gcgcggcgcc gcacgggccg ccgcgcagag cgtccacacc gccgcgttct ccatcacctc
      901 gtcgttcacg aacgcgcgaa gcctccggtc gcgggccggc tcctcggtcg ggtcctgcca
      961 cttcgcctgc acccacgggc tgtacgggaa cgcgtagaac tcgaagtgac ggtgctccgc
     1021 caggtggcgg tccaggtcgg caaccacctc gtccaggcgc atccgctcgg agcgataccg
     1081 caggcggtag gccggcagga cccgcagcgt gacgagcacg atcaccccga gggcccccag
     1141 cgagacgcgg gccgccgaga gcaggtccgg gtcggacttc tcggagcact ccacgacgtc
     1201 cccgaccccg gtgaccagcg tcaggccggt gacctgcgcg gccagccccc cgagcgccgc
     1261 gccggtcccg tgcgtgcccg tggaggtcgc gccggcgatc gactgtgcgt cgatgtcccc
     1321 gaggttctct ggcgccgcgc cgtggccgag caacgcgtcg ccgagcgcgc ggagccgggt
     1381 cccggcccgg accgtggcct gcccggcggc aggatcggcc gccagcaggc cgtggaaccc
     1441 gtcgagcgag acgagggtgc cctcggtcac cgcgagcggg gtgaacgagt gtcccgcgcc
     1501 ggccactcgc agggtctccc ccgcgtcgcg cgcggcacgg acgagcgcga cgacctcctc
     1561 gatgctctcc ggccgggcca gccgccgcgg cgtgaaccgc accgaccccg accagttccg
     1621 ccatccgggc cctcgcacgg cccgcgccac gccgctcaca ggaacgtcag ggcgtcaccg
     1681 cggtacgtcg gaacctcgcc cgccatgccg tcggcggtgg cgagcagcag gcggtcgaag
     1741 cgctcgcaca gctcgccggc cttggcgtgg cggaggaaca ccgggtcccc gatcgccagc
     1801 cgctccgggc cgcggtagcg gatcggggtc tgcacctcgc cggcctcctc gttcgtcagc
     1861 agccgggcgc ccgccggaag gtacggaacc ggctgcttcg aggccccggt cgcgcccgat
     1921 gcgatgtagc cgccgccgag gcacgtgtag acgtccggcg ccggacggcg caccacgggg
     1981 atggcgaacg ccgcggcggg caggtgccgg aacgcgtcgt agtggtcgaa cagcccgggg
     2041 gcgaagaacg ctgagccggc ggccgcctcg gtgacgacgt cctcggccgt ggtcgtctcc
     2101 aggctgccgg tgccgccccc gttcacgaag cgcagcggtc cggcgaccgc ctcgacggcg
     2161 cgcacgatcg ccgcgcgacg ctcgcgcacc gtgcgcaccg agcgccgctt cagtgcccgg
     2221 atgacgggac cccgcaggcc ctggccgggc gtgcggtcgg ggagcccggc gacctgggcc
     2281 tcgtagccca tgatcccgtc caggagcaga cggtccga
//
LOCUS       DASPWO010000602         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_676607, whole genome shotgun sequence.
ACCESSION   DASPWO010000602 DASPWO010000000
VERSION     DASPWO010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676607"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VEO00_13690"
     CDS             <1..1432
                     /locus_tag="VEO00_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HYU59083.1"
                     /translation="RDIKPENILVTPDGVTKVADFGLARALAESTTTHAPGTVTGTVQ
                     YLAPEQVQGEPSDPRTDLYAVGIVLFELLTGHVPFTGETSLAIAYKHLSSRVPAPSKL
                     VAGITPAIDRIVLQATEKDREKRPPSASRMRKDLVRAATSLPPARPVGEVVGDMPASD
                     IVPEDRAPTVTIPRTLSKRTKRRRALRRALWRVAIVAVVILGMWATWTFAVPHRVEVP
                     RLVGLGVPAAARAAEDAGLQVEVTREVHSSSVPDGQIISQDPAAGARIERGSTISLVR
                     SLGPDLRQVPPVEGRDRETAVRLLREAGFVVKVERAFDETAAPGTVIDQDPGAGSSYE
                     RGRTVTITVSRGRPFVDVPSVVGETRESAVALLSSANLEFTEREAFSGAVEQGRVISQ
                     SPDGGTNVREGETVTIVVSKGPRVFPMPNVVGKSEAQARSQLEDMGLEVRTVQVPGSL
                     GGSVVSQVPEPGTQVEQGDRVTIYVA"
     gene            1436..2287
                     /locus_tag="VEO00_13695"
     CDS             1436..2287
                     /locus_tag="VEO00_13695"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HYU59084.1"
                     /translation="MRIGAHLATGGRTSGAVDAARGADADAVQIFVSNPRAWAARAVT
                     PEEAATTRARLAEADVALFVHAAYLVNVASPNPAFLSKSVASARADLAVAEAVGAGGL
                     VLHGGAGGPTERPAALRRAARVIREIAAGARATEVLVELTAGGAGTVASTLPEAAELF
                     DAIGEGGRVALCIDTCHLFAAGYGLDEPDGVGAAFAELDDLGLASRLRLVHANDAAFE
                     RGSRRDRHARVGTGFIGEAGFRAILAQPALAERSVVIETSGSPDDHRDDVARLRALAP
                     ESARRTG"
BASE COUNT          296 a          918 c          930 g          310 t
ORIGIN      
        1 ccgcgacatc aagccggaga acatcctggt gacgcccgac ggcgtgacga aggtcgccga
       61 cttcggcctg gcccgcgcgc tcgccgagag caccaccacc cacgccccgg ggacggtcac
      121 cgggaccgtg cagtacctcg cgcccgagca ggtccagggc gagccgtcgg acccgcgcac
      181 cgacctctac gcggtgggca tcgtcctgtt cgagctgctg accgggcacg tcccgttcac
      241 cggcgagacg tcgctggcga tcgcctacaa gcacctctcg tcgcgcgtgc cggcgccgtc
      301 gaagctcgtc gccggcatca cgcccgcgat cgaccggatc gtgctgcagg cgaccgagaa
      361 ggaccgcgag aagcggccgc cgtcggcctc gcggatgcgc aaggacctgg tgcgcgcggc
      421 gacgagcctc ccccccgcgc ggccggtcgg ggaggtggtc ggcgacatgc cggcctcgga
      481 catcgtcccc gaggaccgcg cgccgaccgt gacgatcccg cgcaccctct cgaagcgcac
      541 caaacgccgg cgggcgctgc gccgcgcact gtggcgtgtg gcgatcgtcg ccgtcgtgat
      601 cctggggatg tgggccacgt ggacgttcgc ggtcccgcac cgcgtcgagg tcccgcgcct
      661 ggtcgggctc ggcgttccgg ccgccgcccg cgccgccgag gacgccgggc tgcaggtgga
      721 ggtgacccgg gaggtccatt cctcgagcgt gcccgacggg cagatcatca gccaggatcc
      781 cgcggccggc gcgcgcatcg aacgcggctc gaccatctcg ctggtccgct cgctggggcc
      841 cgacctgcgt caggttcccc cggtcgaggg ccgcgaccgc gagacggcgg tacggctctt
      901 gcgcgaggcg ggcttcgtcg tcaaggtgga gcgcgccttc gacgagaccg ccgcgccggg
      961 cacggtgatc gaccaggatc ccggcgcggg gagctcctac gagcgcggca ggacggtgac
     1021 catcacggtc agccgcgggc ggcccttcgt cgacgtcccc tcggtggtcg gcgagacgcg
     1081 cgagagcgcc gtcgccctgc tgtcctcggc gaacctcgag ttcaccgagc gagaggcgtt
     1141 cagcggcgcc gtcgagcagg ggcgggtcat ctcgcagagc cccgacggcg ggaccaacgt
     1201 gcgagagggc gagaccgtga cgatcgtcgt ctcgaagggt ccgcgggtgt tcccgatgcc
     1261 gaacgtggtc gggaagagcg aggcgcaggc gcgttcccag ctggaggaca tggggctcga
     1321 ggtgcgcacc gtgcaggtcc ccggctcgct cggcggctcc gtggtcagcc aggtcccgga
     1381 gcccggtacc caggtcgagc agggcgaccg cgtgacgatc tacgtcgcct gacgggtgcg
     1441 tatcggcgcg cacctcgcga cgggggggcg gacctccggc gccgtcgatg cggcccgggg
     1501 cgccgacgcg gacgccgtcc agatcttcgt ctcgaacccc cgcgcctggg ctgcgcgcgc
     1561 ggtgaccccc gaggaggcgg ccacgacccg ggcgcgcctg gccgaggccg acgtcgcgct
     1621 cttcgtccac gccgcctacc tggtgaacgt cgcctcgccg aaccccgcgt tcctctcgaa
     1681 gagcgtggcc tcggcgcgcg ccgacctggc ggtggcggag gcggtcggcg ccggagggct
     1741 cgtgctccac ggcggcgccg gcgggccgac cgagcgaccc gccgcgctgc gccgggccgc
     1801 gcgggtgatc cgggagatcg ccgccggggc gcgcgcgacc gaggtgctgg tcgaactgac
     1861 cgccggcggg gcggggaccg tcgcgtccac gctgcccgag gcggcggagc tcttcgacgc
     1921 gatcggcgag ggcggccggg tcgcgctctg catcgacacc tgccacctgt tcgccgccgg
     1981 ctacggcctc gacgagcccg acggcgtcgg cgcggccttc gccgagctcg acgatcttgg
     2041 gctcgcgtca cggctgcggc tggtccacgc gaacgacgcg gcgttcgagc gcggctcgcg
     2101 acgcgaccgc cacgcccggg tcgggaccgg cttcatcggc gaggccggct tccgggcgat
     2161 cctggcgcag ccggcgctgg ccgagcgctc cgtggtcatc gagacgtccg gctcgcccga
     2221 cgaccaccgg gacgacgtcg cgcgcctccg cgccctcgcg ccggagtcgg cccggcggac
     2281 cgggtgagca ccggtcgaga cgccctgctg ccgatctccc cggtgcgccg ccacgcgctc
     2341 gaggtggggc tgctcggcat cgccgccgtg tggggcgcga cgttccccgc ggtgaaggag
     2401 gcggtcgggg atgtgccgcc cctcgagttc ctcgcgctcc ggttcctggc cgcc
//
LOCUS       DASPWO010000603         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_84557, whole genome shotgun sequence.
ACCESSION   DASPWO010000603 DASPWO010000000
VERSION     DASPWO010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84557"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..645)
                     /locus_tag="VEO00_13700"
     CDS             complement(79..645)
                     /locus_tag="VEO00_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59085.1"
                     /translation="MNLTDREAWTVIHGMVLGAAFLLAFGGGMAGLWSLKPGLVTATG
                     IVERTRRLVIGTWAMAIVAWATVITGTWIVYPWYREKLAGDDLTGCAGATLPSATCSA
                     RDFLTSNASGDTASWHTFGMEWKEHIAWIAPMLATAVAFGVSYYGRDLIKHQGVRRAL
                     MWTFIAAFATAGVAGVFGAFINKVAPIR"
     gene            786..1724
                     /locus_tag="VEO00_13705"
     CDS             786..1724
                     /locus_tag="VEO00_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYU59086.1"
                     /translation="MGRRGDAVIRTESLTKSYGRARGIVDLSLEVERGEVFGFLGPNG
                     AGKTTTIRTILDFIRPTAGRATVLGLDSVRDSVEIHRRTGYLPGELALWERMTGRELL
                     EYLGSLRGGLDRAAVDRLDERLDVDLDRRIGDLSHGNRTKLGLIVAFAHRPDVVILDE
                     PTQGLDPLVQQEFYRLVEETRDDGRTVFLSSHVLPEVERVCDRVGAIREGRLAAVERV
                     ADLAARAVRHVTIRFAGPVPLDAFRALDGVSAVRVDGDALRLTAGGPIDALVKTAARF
                     EVRDLVSEKPNLEELFLALYGRQEPAEAPAEATGGG"
     gene            1726..>2351
                     /locus_tag="VEO00_13710"
     CDS             1726..>2351
                     /locus_tag="VEO00_13710"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYU59087.1"
                     /translation="MLRSVFGKTLWEGRRALVWWSLGMLLLAMTALGFYPSIKASAAD
                     LQKLIQGLPQGLRHLFLGNAPDFTSPAGYLNGRVFSTIAPILLLVYAVGAGARALAGE
                     ERGHTLDLLLSTPLPRRRLALEKLAAMATGLFVLALVLWVALAALGPAFELDVSPGRV
                     AAATFSSYLFALALGAVALAVGGATGSRGSAIGIPTTLAAAGFLLVSLA"
BASE COUNT          310 a          866 c          807 g          368 t
ORIGIN      
        1 gctcgctcga cggcgcgctc cgcggtggac tcggttccca tgtcggtcat cgtctcgtcg
       61 gacatcgtca gaccctcctc accggatcgg ggctaccttg ttgatgaagg cgccgaagac
      121 gcccgcgacc cccgccgtcg cgaaggccgc gatgaaggtc cacatgaggg cccgacggac
      181 cccctggtgc ttgatcaggt ccctgccgta gtacgagacg ccgaaggcca cggcggtggc
      241 gagcatcggc gcgatccagg cgatgtgctc tttccactcc atcccgaagg tgtgccagct
      301 cgccgtgtcc cccgatgcgt tggaggtcag gaagtcgcgg gccgagcacg tggcgctggg
      361 cagcgtggcg cccgcgcaac cggtcagatc gtcacccgcg agcttctccc gataccaggg
      421 atagacgatc caggtccccg tgatcaccgt cgcccacgcg acgatcgcca tggcccacgt
      481 cccgatcacc agccgccggg tccgttccac gatccctgtc gccgtgacga gacccggctt
      541 gaggctccac agcccggcca tcccgccgcc gaacgccagc aggaaggccg ctccgaggac
      601 catgccgtgg atcaccgtcc acgcctctct atccgtcagg ttcatccggt atccccctcc
      661 cggccctcct cggtgggcct ttcgccagat ttcattccct cggaccctcc aaagcaagcg
      721 tccgaacggt ccgattccgg cgggctctga ggccccccgc gccaccctca ctagactgcc
      781 gggccgtggg caggcgaggc gacgccgtca tccgcaccga gtccctgacg aagtcctatg
      841 ggcgagcgcg cgggatcgtc gacctctcgc tcgaggtcga acggggagag gtgttcgggt
      901 tcctcgggcc gaacggcgcc ggcaagacca ccaccatccg cacgatcctc gacttcatcc
      961 ggccaacggc cgggcgggcc accgtgctcg gactcgactc cgtccgcgac agcgtggaga
     1021 tccaccgccg gacggggtac ctgcccggcg aactggcgct gtgggagcgg atgaccggtc
     1081 gggaactgct cgagtacctg ggcagcctgc gcggcggcct cgaccgggcc gccgtggacc
     1141 gcctagacga gcggctggac gtggacctcg accgccggat cggcgacctt tcgcacggca
     1201 accggacgaa gctcggcctg atcgtcgcct tcgcgcaccg gcccgacgtg gtgatcctgg
     1261 acgagccgac ccagggactc gatccgctgg tacagcagga gttctaccgg ctggtggagg
     1321 agacccgtga cgacggccgg acggtcttcc tctcctccca cgtcctcccg gaggtcgagc
     1381 gggtgtgcga ccgcgtcggg gcgatacgcg agggacgcct ggccgccgtg gagcgcgtgg
     1441 cggacctggc ggcccgcgcg gtgcgccatg tcacgatccg cttcgccggg cccgtcccgc
     1501 tggacgcgtt ccgcgctctc gatggcgtct cggccgtgcg cgtcgacggc gacgccctgc
     1561 gcctgacggc cgggggcccg atcgacgcgc tggtcaagac ggcggcgcgc ttcgaggtgc
     1621 gcgacctggt cagcgagaag ccgaacctgg aggagctgtt cctggcgctc tacggacgcc
     1681 aggaaccggc agaggccccg gcggaggcga cggggggagg ctgagatgct ccgctcggtc
     1741 ttcgggaaga cgctctggga gggccggcgg gccctggtgt ggtggtccct gggcatgctc
     1801 ctgctggcca tgaccgccct ggggttctat ccgagcatca aggcgagcgc cgcggacctg
     1861 cagaagctga tccagggcct gccccagggg ctccgtcacc tcttcctcgg caacgccccc
     1921 gacttcacct cgccggctgg ctacctgaac ggccgcgtgt tctcgacgat cgctccgatc
     1981 ctgctcctcg tctacgccgt cggcgccggc gcccgcgctc tcgccggcga ggagcgcggc
     2041 cacaccctgg acctgttgct ctcgaccccg ctcccacggc gccggctggc gctggagaag
     2101 ctcgcagcga tggcgaccgg gctgttcgtc ttggcgctgg tcctgtgggt ggcgctggcg
     2161 gcgctcggcc cggcgttcga gctcgacgtg tccccgggcc gggtggccgc cgccaccttc
     2221 tcgtcgtatc tgttcgcctt ggcgctcggg gccgtggcgc tcgccgtcgg cggcgcgacc
     2281 ggcagccgcg ggtcggcgat cgggatcccg accacgcttg ccgcggccgg gttcctgctc
     2341 gtctcgctcg c
//
LOCUS       DASPWO010000604         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_677005, whole genome shotgun sequence.
ACCESSION   DASPWO010000604 DASPWO010000000
VERSION     DASPWO010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677005"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VEO00_13715"
     CDS             <1..1021
                     /locus_tag="VEO00_13715"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HYU59088.1"
                     /translation="EAEERFKEVSQAYDVLGDEKKRPEYDRVRDMVSSGGFSGGFPGG
                     FPGGGGGQRIRFEDLGDLGFEGDLGGLFGSLFGRGASRAGGASRAGAASHGADLEAEV
                     RISFDQAMSGTTVPLKIRGAAQCPTCHGSGARPGTKVQTCPQCGGRGVSIEDQGLFSM
                     SRPCPRCGGSGRLIETPCETCRGTGVTDRTRTVTVKVPPGIRDGARIRVAGRGEAGRG
                     GANAGDLYVRVRVKPHTVFGRSGSDLTVTLPVSYPELALGAQVPVPTLNGPVTLKVPA
                     GTANGRTFRIRGKGAPKPSGGHGDLLVTVSVQVPQRLSREEKDLLKRLQETARGDPRA
                     GLGAS"
     gene            1038..1430
                     /locus_tag="VEO00_13720"
     CDS             1038..1430
                     /locus_tag="VEO00_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYU59089.1"
                     /translation="MAERDDERRRNRERAVYIISVAAELSGVHPQTLRIYERRGLLAP
                     ARTSGNTRRYSERDIERLREIQQLTQEHGVNLAGVKMIMELERELERLRGAIEEMRRQ
                     MAAIQRRSSAAGALVRRSEVADLLAQLR"
     gene            1554..>3359
                     /locus_tag="VEO00_13725"
     CDS             1554..>3359
                     /locus_tag="VEO00_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HYU59090.1"
                     /translation="MDLNKLTLKSQQALADAQREAAGRNHQSVEPEHLLLALLSDPEG
                     VVYPVLHRVGISPASLRDRVEEALDRIPKVYGAAEPGSSIAPAARHILERAEKEAASL
                     TDEYVSTEHLLLAMLEGANATARLLVDAGLTREALLAALAEVRGRQRVTDQNPEDKYR
                     ALERYGRDLTELARRGKLDPVIGRDDEVRRVIQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIVSGDVPDSLKDKRVVALDIGALVAGSKYRGEFEERFKAVLREIAESEGRIVT
                     FIDELHTVVGAGAAEGSVDAGNMLKPLLARGELRAIGATTLDEYRKHVERDPALERRF
                     QPILVAEPSVEDTIAILRGLKERYEVHHGVRIQDPALVAAAMLADRYVTGRFLPDKAI
                     DLIDEAASRLRIEIDSMPVEIDELDRRIKQLDIERAALSKESDEASKERLGKLEAERA
                     DLEERMAGMQAHWREERERIDRLRALKADLEEARQEFERQERPGGDIERAAELRHGRI
                     PALEAELGEESDRLADLQRDRKMLKEEVDEEDVAEVVSKWTHIPVSRMMEGEVQKLIH
                     MEERLHKRVVGQDEAVEAVSNALRRARSGLQDPDRP"
BASE COUNT          530 a         1114 c         1317 g          398 t
ORIGIN      
        1 ggaggccgag gagcggttca aggaggtctc gcaggcctac gacgtcctcg gggacgagaa
       61 gaagcggccg gagtacgacc gggtgcgcga catggtgtcc tcgggcgggt tttccggcgg
      121 gttcccgggc ggcttcccgg gcggaggcgg cgggcagcgc atccggttcg aggacctcgg
      181 ggacctcggg ttcgagggcg acctcggcgg actattcggg tcgctgttcg ggcgtggcgc
      241 gagccgcgcc gggggcgcca gccgcgccgg cgccgcgagc cacggcgccg acctggaggc
      301 ggaggtgcgc atctcgttcg accaggcgat gtccgggacc acggtgccgc tcaagatccg
      361 cggcgcggca cagtgcccga cctgtcacgg gtcgggggca cgcccgggga cgaaggtgca
      421 gacctgcccg cagtgcggcg ggcgcggcgt cagcatcgag gatcaggggc tgttctcgat
      481 gtctcggccg tgcccgcgct gcggcgggtc cgggcggttg atcgagacgc cgtgcgagac
      541 ctgccgcggg accggcgtca ccgaccggac gcgcacggtg accgtcaagg tcccgccggg
      601 gatccgcgac ggcgcccgga tccgggtggc cggccgtggc gaggcgggcc ggggcggcgc
      661 gaacgccggt gacctctacg tccgggtgcg ggtcaagccg cacacggtct tcgggcggtc
      721 ggggagcgac ctgaccgtga cgctcccggt gagctacccg gagctcgcgc tcggcgcgca
      781 ggtcccggtg ccgacgctga acggcccggt gaccctgaag gtcccggccg gcaccgccaa
      841 cgggcggacg tttcgcatcc ggggcaaggg ggcgccgaag ccctccggcg ggcacggcga
      901 cctgctggtc acggtgtcgg tgcaggtccc gcagcgactc tcgcgtgagg agaaggacct
      961 gctgaagcgg ctgcaggaga cggccagggg cgatccccgg gccggcctcg gggcatcctg
     1021 agaggagaga gcgagcgatg gcggagcgtg acgacgagag gcggcgaaac cgggagcgcg
     1081 cggtgtacat catcagcgtg gcggccgagc tgtcgggcgt gcacccccag acgctccgca
     1141 tctacgagcg gcgggggctg ctcgcgccgg cccgcacctc gggcaacacg cggcggtact
     1201 cggagcgcga catcgagcgg ctccgtgaga tccagcagct gacgcaggag cacggggtca
     1261 acctggccgg ggtgaagatg atcatggagc tcgagcgcga gctcgagcgt ctgcgcggtg
     1321 cgatcgagga gatgcgacgg cagatggcgg cgatacagcg ccgctcgagc gccgccggcg
     1381 cgctggtgcg ccgctccgag gtggcggacc tgctggcgca gctccgctga gggctatcgt
     1441 gcttggaggg ttcgcgcgcc gtccgttgac gagccgatcg acggccgggg cgagctacgc
     1501 gcccaaaccc cccgagacca cgagcgcgag aacgagagga cgaaccgaac gccatggacc
     1561 tcaacaagct gaccctgaag tcacagcagg cgctggccga cgcgcagcga gaggcggccg
     1621 ggcgcaacca ccagtccgtc gagcccgagc acctgctgct cgcgctgctc tcggacccgg
     1681 agggcgtcgt ctacccggtg ctgcaccgcg tcgggatctc gcccgcctcg ctgcgcgacc
     1741 gggtcgagga ggcgctggat cggatcccga aggtgtacgg cgccgcggag ccgggctcgt
     1801 cgatcgcgcc ggccgcgcgg cacatcctgg agcgggccga gaaggaagcc gcatcgctca
     1861 ccgatgagta cgtctcgacc gagcacctgc tgctggcgat gctcgagggg gcgaacgcca
     1921 ccgcgcgcct gctggtcgac gccgggctga cgcgcgaggc gctgctcgcg gcgctggccg
     1981 aggtccgtgg gcgccagcgg gtgaccgacc agaaccccga ggacaagtac cgggcgctgg
     2041 agcgctacgg ccgcgacctg accgagctgg cccggcgcgg gaagctcgac ccggtgatcg
     2101 gtcgcgacga cgaggtgcgc cgcgtgatcc aggtgctgtc gcgccgaacg aagaacaatc
     2161 ccgtgctgat cggcgagccg ggggtcggga agaccgccat cgtcgagggc ctggcccagc
     2221 ggatcgtctc cggcgatgtg cccgactcgc tgaaggacaa gcgggtcgtg gcgctcgaca
     2281 tcggcgcgct cgtcgccggc tcgaaatacc gcggcgagtt cgaggagcgg ttcaaggccg
     2341 tgctccgtga gatcgccgag agcgagggcc ggatcgtcac gttcatcgac gagctgcaca
     2401 ccgtcgtcgg cgccggcgcg gccgagggat ccgtggacgc cgggaacatg ctgaagccgc
     2461 tcctggcgcg cggcgagctg cgcgccatcg gcgcgaccac gctcgacgag taccgcaagc
     2521 acgtcgaaag ggacccggcg ctcgagcggc gcttccagcc gatcctcgtc gccgagccgt
     2581 cggtcgagga cacgatcgcc atcctgcgcg ggctgaagga gcgctacgag gtccaccacg
     2641 gggtgcgcat ccaggacccg gcgctcgtcg cggccgccat gctcgcggac cgctacgtca
     2701 ccggccggtt cctccccgac aaggcgatcg acctgatcga cgaggccgcc agccggctgc
     2761 gcatcgagat cgactcgatg ccggtcgaga tcgacgagct cgaccgccgc atcaagcagc
     2821 tcgacatcga gcgggccgcg ctctcgaagg agtccgacga ggcctcgaag gagcgcctgg
     2881 gcaagctcga ggccgagcgg gccgacctcg aggagcgaat ggccgggatg caggcgcact
     2941 ggcgggagga gagggagcgc atcgaccgcc tccgcgcgct gaaggcggat ctcgaggagg
     3001 cgcggcagga gttcgagcgc caggagcgtc ccggagggga catcgagcgc gccgcggagc
     3061 tccgccacgg gcggatcccg gcgctggagg ccgagctggg cgaggagagc gaccgcctgg
     3121 ccgatctgca gcgcgaccgc aagatgctga aggaggaggt ggacgaggaa gacgtcgccg
     3181 aggtcgtgag caagtggacg cacattccgg tcagccggat gatggagggc gaagtccaga
     3241 aactgattca catggaggag cgcctccaca aacgcgtggt cggccaggac gaggcggtcg
     3301 aggcggtctc gaacgccctg cggcgggccc gctcggggct tcaggacccg gaccggccg
//
LOCUS       DASPWO010000605         6855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1185474, whole genome shotgun sequence.
ACCESSION   DASPWO010000605 DASPWO010000000
VERSION     DASPWO010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185474"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VEO00_13730"
     CDS             <1..879
                     /locus_tag="VEO00_13730"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYU59091.1"
                     /translation="RDPLREVVVGILSDAHVLIDDVPGVGKTTLIRMLAGLLGLDFQR
                     VQFTPDLLPSDVTGINYFSQQSQRFEFRAGPVFANVILADEINRATPRTQSALLESMG
                     EHQVTVEGEPRVLARPFLVIATQNPIEYEGTFPLPEAQLDRFLLRVRMGYPDPDQEER
                     ILTVPPSARRVAVRPLPALDLSALRALAAAVRIESPVRRYVVALARATREHHDVQVGA
                     SPRAVEHLGDAARAWALLDGRDYVLPDDVKALAPAVLTHRLVMTTDARIRDRAAEAVL
                     AEVLEELPVPVEFQGA"
     gene            912..2066
                     /locus_tag="VEO00_13735"
     CDS             912..2066
                     /locus_tag="VEO00_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYU59092.1"
                     /translation="MDSPDRRAIAAFVATLSGVVAVASGRRPLLVWALAVGAMAAVAA
                     WWARRAARGVEVSASFRPARAFAGETVRLVVQVRNAKRLPVPTARVAVWLPSGLSPAA
                     GHASTAIRGFARRVALPGRSEVVLEFPVDARRRGEYWLERVELELSDPFDLTPIRRTA
                     VPEADLLVFPEPHGPVPVRVLRRLPFGMPAPAARMFEDRERFAGVRPYEPGDPLNRIH
                     WKLTGHAGALQTKLFEPTRRAEVLLALDLAVGEPFWDAVYPEIAEDGIGWASYLARLA
                     LRSGWRAGLLANTHLSRGRGLLRVPPALAAGHEAVLFAALARMPNEPTCDLGPVLRET
                     SRGFSRETSVLVISPRPGPSLRAEVGALRRRGIRVHETSPLERWLAEDRA"
     gene            2063..3412
                     /locus_tag="VEO00_13740"
     CDS             2063..3412
                     /locus_tag="VEO00_13740"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HYU59093.1"
                     /translation="MRAPVNGSPTALPGARPRAVLAAALAATAEAGIVYLPVAMIARE
                     AGDTGGPLVHYPLFLILFVAATTLATRFRASPRLAGAAGAIAVALGLVQAVVWWTPRP
                     VDLAGSVLLVLVVALRIVTLGLRDWRSPVNGSFALGAVILLAEIATASPATGWAPLLP
                     AVVPVFFLASLASRAASVRLVGTPADGGPAVPRRVGAPLLVGGLALTMGVAGVLGGPR
                     GALAGLGRAAFPILSQLLSILLRVVGVLSWPFIWLLQRLGLDASVLQHRLRDASEEVV
                     RGARSGEPPSPLLGRIVGVLVLVGLIALLLVTIRAIGRSRARVRRVLDEFGPPPGALP
                     GTRGPGRHRRPRLRRELPVETVRRLYAEVLLLLERHGMVRGSSVTPQEHLAAGGAAFP
                     DLARDLAVLTRAYEDVRYGYVAIPEAVVQDLRRRHARITEVLRARRAPEPAAPAGAP"
     gene            complement(3351..4100)
                     /locus_tag="VEO00_13745"
     CDS             complement(3351..4100)
                     /locus_tag="VEO00_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HYU59094.1"
                     /translation="MIWIGTSGWQYRDWRRRFYPRDLAQRDWLPFFSERFATVEVNNS
                     FYRLPEADTFAAWRDRSAAGFRFAVKASRYITHVRRLRDAAGPVRTFMERARRLGDKL
                     GPVLYQLPPNFPADPDRLAAFLEGLPRDPPAAFEFRHPSWDDTGVRGLLDAHDCAWVL
                     ADRPGWRYREVVTGGWAYVRFHQGRRTAPGYARRKLATWAGRLADLPARDVYVYFNND
                     TGGAALRDGAALTELLRARRVPVRGAREAPP"
     gene            complement(4097..4756)
                     /locus_tag="VEO00_13750"
     CDS             complement(4097..4756)
                     /locus_tag="VEO00_13750"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYU59095.1"
                     /translation="MNAVVRFETADRVVLEGELRPGDPPVRGTAVVCHPHPQHGGSKD
                     HPLLWAIRSALATGAHLTTLAFNFRGVMGSGGSYGGGVAEVEDVRAAIGRVRHDVGGP
                     TVVVGWSFGAQTALREAIEDDRVAALALVGVVLGSRAASFTSLPPLPTLDRLNEFDRP
                     VLLLSGSRDRFSPPDHVRALAIQLPRAETLIVPDGDHFFWRREREVAALVADFLERAL
                     P"
     gene            4778..5785
                     /locus_tag="VEO00_13755"
     CDS             4778..5785
                     /locus_tag="VEO00_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYU59096.1"
                     /translation="MGGGQATRLGLSLREPLPWSALVEVARTAEDTAYEAVFVPEIAG
                     RESFATLAALAEATDRLSLGTGVVTIAARSPVLTAMAAGTVHELSGGRLILGVGAGSR
                     ASAVGVHSAAAWDHPLDRTRAFLRLVRATLAGGEVRAGPPYPVAGFRSALSLDTIPPV
                     WLAALGDRMVALAGEEADGALLNICTPERVRQARALAREAAAGAGRDPADVTIAVYVR
                     ACLLGTRDDLALDALRAAIGAYVVLPPYRRQFEAMELSTEAEAAAKAMEAGRPVDVPA
                     ELVDAVAVRGGRAEWEARLGAYRAAGADLVVVYPVPAGDPVSSLIGTVLAAAPATAVE
                     R"
     gene            5923..6252
                     /gene="fdxA"
                     /locus_tag="VEO00_13760"
     CDS             5923..6252
                     /gene="fdxA"
                     /locus_tag="VEO00_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HYU59097.1"
                     /translation="MTYTICEPCIDVKDLACVDECPVDCIYEGGRMLYIHPDECVDCG
                     ACEPVCPVTAIFYEDDVPSEWKQFTPINAEFFADSVSGLGSPGGAAQVGPVDKDHPVV
                     AGWATDA"
     gene            6374..>6855
                     /locus_tag="VEO00_13765"
     CDS             6374..>6855
                     /locus_tag="VEO00_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59098.1"
                     /translation="MAVGATEMDFDLDRYLRVSKRVDLSAVAWDRIAEHPLSVDEARC
                     LAYMMDIETHTVIFLRDLLVTRAAREPEVTAFLACWVYEELWHGEAFSRFLGEAGYEL
                     SPDGERPWSDAPYPTRVARNAWIREALSGKGPYAHLVTMLGSALMKEFVALHMTWGAA
                     "
BASE COUNT          797 a         2511 c         2585 g          962 t
ORIGIN      
        1 cgggacccgc tgcgtgaggt cgtcgtcggc atcctctccg acgcgcacgt gttgatcgac
       61 gacgtcccgg gggtcggcaa gaccacgctg atccggatgc tcgccggact gctcgggctc
      121 gacttccagc gggtacagtt cacgcccgac ctgctgcctt cggacgtgac cggcatcaac
      181 tacttcagcc agcagtcgca gcgcttcgag ttccgggcgg ggccggtctt cgccaacgtc
      241 atactggcgg acgagatcaa ccgcgccacg ccgcgcacgc aatccgcgct gctggagagc
      301 atgggcgagc accaagtcac cgtcgagggt gagccgcgcg ttctggcgcg gccgttcctc
      361 gtgatcgcga cgcaaaaccc catcgagtat gaaggcacct tcccgctgcc ggaggcccag
      421 ctcgaccggt tcctgctcag ggtccggatg gggtacccgg accccgatca ggaggagcgg
      481 atcctgacgg ttccgccctc cgcgcggcgg gtcgccgtcc ggcccctgcc cgctctcgac
      541 ctgtcggcgc ttcgcgcgct cgccgcagcg gtgcggatcg agtcgccggt ccgccggtac
      601 gtcgtcgcgc tggcccgcgc cacgcgcgag caccacgacg tgcaggtcgg tgccagcccg
      661 cgcgccgtcg agcacctcgg cgacgccgcg cgcgcctggg cgctgctcga cggccgcgac
      721 tacgtgctgc ccgacgacgt gaaggccctg gcgccggcgg tgctcacgca ccgcctggtg
      781 atgaccaccg acgcgcgcat ccgcgatcgc gccgccgagg ccgtcctggc ggaggtcctg
      841 gaggagctcc cggtccccgt cgagttccag ggcgcctagc gaggcccggc cggccggaac
      901 gggaggagcg cgtggacagc cccgaccggc gcgcgatcgc ggcgttcgtc gccaccctgt
      961 ccggcgtcgt cgcggtggcc tccggtcgcc gcccgctgct ggtctgggcc ctggccgtcg
     1021 gggcgatggc ggcggtcgcg gcgtggtggg cgcgacgggc agcgcgcggg gtggaggtct
     1081 cggcgtcgtt ccgtcccgcc cgggccttcg ccggtgagac cgtccgcctc gtcgtccagg
     1141 tgcgcaacgc gaagcgcctg ccggttccga cggcgcgggt cgccgtgtgg ctcccgtccg
     1201 gtctttcgcc cgcggcgggg cacgcgtcga cggcgatccg cgggttcgcc cggcgcgtgg
     1261 cgctcccggg gcgatcggag gtcgtgctcg agttccccgt ggacgcgcgc cgccgtgggg
     1321 agtactggct ggagcgggtc gagctcgagc tctcggaccc gttcgacctg acgccgatcc
     1381 ggcggacggc cgtcccggag gccgacctgc tggtgttccc ggagccgcat ggcccggtac
     1441 cggtccgggt gctgcgacgg ctgcccttcg gcatgccggc gccggccgcg cggatgttcg
     1501 aggaccgcga gcggttcgcc ggcgtccggc cgtacgagcc gggcgatccg ctgaaccgca
     1561 tccactggaa gctcaccggg cacgccgggg cgctgcagac gaagctcttc gagccgacgc
     1621 ggcgggccga ggtgctgctg gcgctcgacc tggccgtcgg cgagccgttc tgggacgcgg
     1681 tctaccccga gatcgccgag gacggcatcg gctgggcgag ctacctggcg cggctggcgc
     1741 tccgatcggg gtggcgcgcc gggctgctgg cgaacaccca cctctcccgc gggcgcgggc
     1801 tgctgcgcgt ccctccggcg ctggcggccg gccacgaggc ggtgctgttc gccgcgctgg
     1861 cccggatgcc gaacgagccg acgtgcgacc tcgggcccgt gctccgggag acctcgcggg
     1921 gcttcagccg cgagacgtcg gtgctcgtga tctcgccgcg cccgggaccc tcgctgcgag
     1981 ccgaggtcgg cgcgctgcgc cgtcgcggga tccgcgtcca cgagacctct cccctcgagc
     2041 gctggctggc ggaggaccgc gcgtgagggc gccggtgaac ggttcgccga ccgcgctccc
     2101 aggggcgcgc ccccgggccg tcctcgcggc cgccctggcc gcgacggccg aggccgggat
     2161 cgtgtacctc ccggttgcca tgatcgcccg agaggccggc gataccggcg gcccgctcgt
     2221 ccactacccg ctcttcctga tcctcttcgt cgccgcgacc acgctcgcca cccggttccg
     2281 cgcctcgccg cggctggccg gcgcggccgg ggcgatcgcg gtggcgctcg gcctggtgca
     2341 ggcggtggtg tggtggaccc cccggccggt cgatctggcc gggtcggtcc tcctcgttct
     2401 ggtggtggcg cttcggatcg tcacgctcgg gctgcgggac tggcggtcgc cggtgaacgg
     2461 gtccttcgcg ctcggggcgg tgatcctgct ggccgagatc gccacggcga gccccgccac
     2521 cggctgggcg ccgctgctgc cggcggtcgt gccggtcttc ttcctggcgt cgctggcgtc
     2581 gcgtgccgcg agcgtccgtc tggtcgggac cccggccgac ggcgggccgg ccgtgccccg
     2641 gcgggtgggc gcgccgctgc tcgtcggcgg gctggcgctc acaatgggcg tcgccggggt
     2701 gctcggaggg ccgcgcggcg cgctcgccgg actgggccgg gccgcgtttc cgatcctctc
     2761 gcagctgctg agcatcctgc tgcgggtggt cggcgtcctg agctggccgt tcatctggct
     2821 gctgcagcga ttggggctcg acgcctccgt gctccagcac cggctgcgag acgcctcgga
     2881 ggaggtcgtt cggggcgccc ggtcgggcga gcccccgtcg ccgctgctgg gccgcatcgt
     2941 cggcgtgctc gtgctcgtcg ggctgatcgc gctcctgctg gtgacgatcc gggcgatcgg
     3001 acgaagccgg gcccgggtgc ggcgcgtgct ggacgagttc gggcccccgc ccggggcgct
     3061 gcccggcacg agggggccgg ggcggcaccg gcggccgcgc ctgcgacggg agcttccggt
     3121 ggagacggtg cggcgcctgt acgccgaggt gttgctgctg ctcgagcgcc acggcatggt
     3181 ccgcggctcc tcggtcacgc cgcaggagca cctggcggcc ggcggggccg cgttccccga
     3241 cctggcgcgg gacctcgcgg tgctgacccg cgcctacgag gacgtccgct acggatacgt
     3301 ggcgatcccg gaggccgtcg tccaggacct ccgccggcgg cacgcgcgga tcacggaggt
     3361 gcttcgcgcg cgccgcgcac cggaacccgc cgcgcccgca ggagctccgt gagcgccgcg
     3421 ccgtcgcgga gcgcggcgcc gcccgtgtcg ttgttgaagt agacgtacac gtcgcgcgcc
     3481 gggagatcgg ccagccggcc ggcccacgtc gcgagcttgc ggcgcgcgta ccccggggcc
     3541 gtccgccgcc cctggtggaa ccggacgtag gcccagcccc cggtgacgac ctcccggtag
     3601 cgccaccccg ggcggtcggc gagaacccac gcgcagtcgt gcgcgtcgag cagcccccgc
     3661 acgccggtgt cgtcccagct cggatggcgg aactcgaagg ccgccggcgg gtcacgggga
     3721 aggccctcga ggaacgccgc cagccgatcg gggtcggccg ggaagttcgg cggcagctga
     3781 tagagcaccg gcccgagctt gtccccgagc cggcgggccc gttccatgaa cgtccgcacg
     3841 gggccggctg cgtcgcgcag ccgacggacg tgggtgatgt agcggctggc cttcaccgcg
     3901 aagcggaacc ccgccgcaga gcgatcgcgc cacgccgcga aggtgtccgc ctccggcagc
     3961 cggtagaagg agttgttcac ctcgaccgtg gcgaagcgct cggagaagaa cggcagccag
     4021 tcgcgctgcg cgaggtcccg gggatagaag cgccgccgcc agtcgcggta ctgccagccg
     4081 ctcgtgccga tccagatcac gggagggccc gctccaggaa atcggcgacc agggccgcga
     4141 cctcgcgctc gcggcgccag aagaagtggt cgccgtccgg cacgatcagc gtctcggcgc
     4201 ggggtagctg gatggcgagc gcgcggacgt ggtcgggtgg gctgaaccgg tcgcgcgatc
     4261 ccgacagcag gagcaccggc cggtcgaact cgttcagccg gtcgagcgtg gggagcgggg
     4321 ggagcgacgt gaaggacgcc gcgcgcgatc ccagcaccac cccgacgagc gccagcgcgg
     4381 caacccggtc gtcctcaatc gcctcgcgca gcgcggtctg cgcgccgaag gaccacccga
     4441 cgaccaccgt cggacccccc acgtcatgcc tgacccgtcc gatcgccgcg cggacgtcct
     4501 cgacctcggc caccccgccg ccgtaggagc cgcccgagcc catcacgccg cggaagttga
     4561 acgcgagtgt cgtcaggtgc gccccggtcg ccagcgcgct gcgaatggcc cacagcaacg
     4621 ggtggtcctt gctcccgccg tgctgggggt gcgggtggca gacgaccgcc gtgccccgca
     4681 ccggggggtc gccggggcgc agctcgccct cgagcaccac ccggtccgcc gtctcgaacc
     4741 ggaccaccgc gttcacctgt gggacgatga ccgagcgatg ggtggcgggc aagcgacgcg
     4801 cctcgggctc tcgttgcgtg agccgctgcc gtggagcgcc ctggtcgagg tggcccgcac
     4861 cgcggaggat acggcctacg aggccgtgtt cgtcccggag atcgccggcc gcgagtcctt
     4921 cgccacgctg gcggcgctcg ccgaagcgac cgatcgcctg tccctcggga cgggggtggt
     4981 gacgatcgcg gcccggagcc cggtgctgac ggccatggcg gccggcacgg tccacgagct
     5041 ctccggcggc cgactgatcc tcggggtcgg cgccgggtcg cgggcgtcgg ccgtcggcgt
     5101 gcactccgcc gcggcgtggg accacccgct cgatcggacg cgcgccttcc tccggctggt
     5161 gcgggccacg ctcgccgggg gcgaggtgcg agctggtccg ccgtaccccg tggcggggtt
     5221 ccgatcggcg ctgtccctcg ataccatccc accggtctgg ctcgccgccc tcggcgaccg
     5281 gatggtggcg ctcgccggcg aggaagccga cggcgcgcta ctgaacatct gcactcccga
     5341 acgggttcgg caggccaggg cgctggcccg cgaggcggcg gccggtgccg gccgcgatcc
     5401 ggcggacgtg acgatcgcgg tctatgtccg tgcttgcctg ctcgggaccc gcgacgacct
     5461 ggcgctggac gccctgcgcg cggcgatcgg cgcgtacgtg gtgcttccgc cgtaccgccg
     5521 gcagttcgag gcgatggagc tctccaccga ggccgaggcg gcggcgaagg cgatggaggc
     5581 cgggcgcccc gtcgacgtcc ccgccgagct cgtcgacgcg gtggcggtgc gcggcgggcg
     5641 cgcggaatgg gaggcgcgcc tcggcgccta ccgggccgcc ggcgccgacc tggtggtggt
     5701 ctacccggtc ccggccggcg atccggtttc gtcgctcatc ggcaccgtcc tggccgccgc
     5761 ccccgcgacc gccgtcgagc gctgacgagg ggccggtgag gacgttcagg cggtcaggac
     5821 gcgcgccggg atcacggtcg ggacggcgtc gcggttgacc gccgggcgga agagggtatc
     5881 ctgtatttgc acggtcagca ccgtacatag ggggaaaact ccatgaccta cacgatctgc
     5941 gagccctgca tcgacgtgaa ggacctggct tgcgtggacg agtgtccggt cgactgcatc
     6001 tatgaggggg gccggatgct ctacatccac cccgacgagt gcgtcgattg tggggcctgt
     6061 gaaccggtct gcccggtgac ggccatcttc tacgaggacg atgtgccgtc cgagtggaag
     6121 cagttcaccc ccatcaacgc cgagttcttc gccgactccg tctcgggcct cggatccccg
     6181 ggcggcgccg cccaggtcgg gccggtggac aaggaccacc ccgtggtcgc cggctgggcc
     6241 accgacgcgt agcccgcatc ctgcgccctg gtcgttgcgc gatcggccgc gtcgcggcat
     6301 ggtttcccat gggaacgcgc cgatccgccg cgcggtgcgc tgcgaatcag cggggacgaa
     6361 cgggagggag agcatggccg tcggcgcaac cgagatggac ttcgacctcg atcgctacct
     6421 gcgcgtctcg aagcgggtgg acctttccgc ggtggcatgg gaccggatcg cggagcaccc
     6481 gctgtccgtc gacgaggcgc gctgcctcgc ctacatgatg gacatcgaga cccatacggt
     6541 catcttcctg cgcgacctgc tggtcacccg cgcggcccgc gagcccgagg tgacggcctt
     6601 cctcgcctgc tgggtctacg aggagctgtg gcacggcgag gccttcagcc gcttcctcgg
     6661 agaggccggc tacgagctgt cccccgacgg ggagcgcccc tggagcgacg ccccctaccc
     6721 cacccgcgtg gcccgcaacg cctggatccg cgaggcgctg tccggcaagg ggccctacgc
     6781 ccacctggtg accatgctcg gctccgcgtt gatgaaggag ttcgtggccc tgcacatgac
     6841 ctggggcgcg gcgaa
//
LOCUS       DASPWO010000606         3406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_677533, whole genome shotgun sequence.
ACCESSION   DASPWO010000606 DASPWO010000000
VERSION     DASPWO010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677533"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(453..1547)
                     /locus_tag="VEO00_13770"
     CDS             complement(453..1547)
                     /locus_tag="VEO00_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYU59099.1"
                     /translation="MVDPRKLPEMPALMIAGPGELHEEDLEVLGHQVIAHYGDVWTEL
                     HRQVIGQLGTLLGAADPPYLIPGTGTMALDAAVANVFEPGQRVVVAGTGFFGTRLREV
                     AEAHGLAATVVPVETGAAADTSRVAEAMDRAHGLLTVQVETATGVRHPVEELAAVAHG
                     AGAAIVVDAIASAGGERLDVDGWGIDALATGTQKGLEAPPGLGIVALGPGGRARIEAR
                     SRPPSSWYLDLMRWDHYREEWGAWHPHPVTMPTNLVLALGSSLNRLLERGIESVVATR
                     AGLARRCRDGLRALGLEPVPRPGVEANLVVAAWADDPAALQNHLLRRGIMVSGGLEPT
                     AGKAIRVGLMGRTATEDMVDRLLTHLAEVL"
     gene            complement(1608..2747)
                     /gene="rodA"
                     /locus_tag="VEO00_13775"
     CDS             complement(1608..2747)
                     /gene="rodA"
                     /locus_tag="VEO00_13775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HYU59100.1"
                     /translation="MDATAGRRVAWERRAFRNIDTVLLVVMGLLTIYGMLMVYSATNR
                     SLVTLGEDPAFYLKKQLTFGLLGLVILVVSATVDYRLFKVYAPIAYVAVLGALLAVMV
                     PHIGTRVSGAQRWITLLGFQFQPSELTKVILILMLAAFLSEVKGGLTLRDVLRAAGIA
                     ALPMALVFLQPDVGTSMAFAAILVALLLVARARARQLGILALIAVVSLFLGLQSGLVK
                     DYQLARIGGFLDPDSDPQRAGYNRQQSEIAIGNGGLTGQGYLHGSQTNLDFVPAQHTD
                     FIFTAIGEELGFSGAMVLLLLFGVLLWRGFRIALAAKDPFGFYVATAIVAMFAFQVFV
                     NVGMTVGIMPITGIPLPFVSYGGTALMTNYLAVGLLLNIHMRRFK"
     gene            complement(2767..>3406)
                     /locus_tag="VEO00_13780"
     CDS             complement(2767..>3406)
                     /locus_tag="VEO00_13780"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HYU59101.1"
                     /translation="FGLGRRTGIDLPGEDRGRVPDVEWLKREIQRNAFFADRTGWLPG
                     DDINMSVGQGNVLVSPLQLATAYSAIANGGTLWAPRLALKVVEPDGTLVRKIQPRSTG
                     KLPFTKRQMDYVRESLRGVVTNGTAAGAFLGFPFSQVSVGGKTGTAEMPPLQDASWFA
                     AFAPVEHPQYVVVCLVEEGGHGSETAAPVVRRILEGLYGFGPGELQAGDAVD"
BASE COUNT          516 a         1252 c         1191 g          447 t
ORIGIN      
        1 cccctcgtgt tcagggggag gcccggttgg gggctggagg gagagggcgc ggtgcggact
       61 gcgagtcccg gtgccagcgg gcggggcctg ccggccccat tccagcggga aacgcatccc
      121 acggatgcac ctcttcctcc gccctcgggc ggggctccgc ggcgctcggc ggaacggatg
      181 ggttccgcgt tcggcggctc gcggtggggc cctcgcccgg cggctcgcgc cgctcggacg
      241 ggcactcctg acgatcaggc gctgcggttg cgcgcgcctt cccctcccca gcgtcgcggc
      301 ccggtcggat cgcgatgttc cgccggtctg cgctgtggca gccctgcggc aagcgccgga
      361 gagggacttc tatcggcaag gataccaggg acccgggtcc tcgccccgag gcgcccgaat
      421 ctcccgaaac ggtcagggaa cctcggcgag ggtcagagaa cctcggcgag gtgggtgagg
      481 aggcggtcga ccatgtcctc ggtggccgtg cggcccatca ggccgacgcg gatcgccttc
      541 cccgcggtcg gctcgagccc gcccgagacc atgatccccc gccgaagcag atggttctgc
      601 agcgccgccg ggtcgtccgc ccacgccgcc accaccaggt tcgcctccac gccgggccgc
      661 ggcacgggtt cgagcccgag ggcccgcagg ccgtcgcggc agcgccgggc caggccggcc
      721 cgggtcgcca ccacgctctc gatgccgcgc tcgagcaacc ggttcaggct ggagccgagc
      781 gcgagaacca ggttcgtcgg catggtcacc ggatgcgggt gccaggcccc ccactcctcc
      841 cggtagtggt cccagcgcat caggtccagg taccagctcg acggcggacg agagcgcgcc
      901 tcgatccggg cacggccgcc gggcccgagc gccacgatcc ccaatccggg gggcgcctcc
      961 aggcccttct gggtgccggt ggcgagggcg tcgatccccc agccgtcgac gtcgagccgc
     1021 tcgccgccgg ccgaagcgat cgcgtcgacg acgatcgcgg cgccggcgcc gtgcgccacg
     1081 gccgccagct cctcgaccgg gtggcgcacg cccgtggccg tctcgacctg gacggtcagc
     1141 aggccgtggg cgcggtccat cgcctcggcc acgcgggagg tgtcggccgc ggcccccgtc
     1201 tcgaccggca ccacggtcgc cgcgagcccg tgcgcctcgg cgacctcgcg caggcgggtg
     1261 ccgaagaagc cggtcccggc gacgacgacg cgctgcccgg gctcgaagac gttcgccacg
     1321 gcggcatcga gggccatggt gccggtgccg gggatcaggt agggggggtc ggcggccccg
     1381 aggagcgtcc cgagctgccc gatcacctgt cggtggagct cggtccagac gtcgccgtag
     1441 tgcgcgatca cctggtgccc gaggacctcg agatcttcct cgtgcagctc ccccggtccg
     1501 gcgatcatga gggctggcat ctctggcagc ttcctcggat ccaccatcgc gagcatccta
     1561 ccaacgcgct ccgctccgcc ctgcaggccc gctgcctccc gaccgggcta cttgaagcgg
     1621 cgcatgtgga tattcaggag gaggccgacg gcgaggtagt tggtcatcag agcggtgccg
     1681 ccgtacgaga cgaagggcag gggaatcccg gtgatcggca tgatgccgac ggtcatcccg
     1741 acattgacga acacctggaa cgcgaacatc gcgacgatcg ccgtggcgac gtagaagccg
     1801 aacgggtcct tggctgcgag cgcgatgcga aagccgcgcc acagcaggac cccgaacagg
     1861 agcagcagga ccatcgcgcc ggagaacccc agctcctccc cgatcgccgt gaagatgaag
     1921 tcggtgtgct gcgccggcac gaagtcgagg ttcgtctgcg agccgtgcag gtacccctgg
     1981 ccggtcagcc cgccgttgcc gatggcgatc tccgactgct gccggttgta gcccgcccgc
     2041 tgcgggtcgg agtccggatc gaggaacccg ccgatccggg ccagttggta gtccttcacc
     2101 aaacccgact gcagcccgag gaacagcgac accacggcga tcagggcgag gatcccgagc
     2161 tggcgcgccc gggcccgcgc gacgagcagc agcgccacca ggatcgcggc gaaggccatc
     2221 gaggtcccga cgtcgggctg caggaacacc agcgccatcg gcagcgcggc gatccctgcg
     2281 gcgcgcagga cgtcacgcag cgtcaggccg cccttcacct cggagaggaa ggcggccagc
     2341 atcaggatca agatcacctt cgtcagctcc gacggctgga actggaagcc gagcaacgtg
     2401 atccagcgct gcgccccgga cacccgcgtg ccgatgtgcg ggaccatcac cgcgaggagc
     2461 gcgccgagga cggcgacgta ggcgatcggc gcgtacacct tgaacagccg gtaatccacg
     2521 gtggccgaga ccaccaggat gaccagcccg agcagcccga aggtcagctg cttcttcagg
     2581 tagaaggccg ggtcctcgcc cagggtcacc aggctgcggt tggtggccga gtagaccatc
     2641 agcatcccgt agatcgtcag caggcccatg accacgagca ggaccgtgtc gatgttgcgg
     2701 aacgcgcggc gttcccaggc gacgcggcgt ccggcggttg cgtccatcag gtaccccagc
     2761 ccacccctag tccaccgcgt ccccagcctg cagctcgccg gggccgaagc cgtacaggcc
     2821 ctccaggatc ctccggacca cgggcgccgc ggtctcggag ccgtgcccgc cctcctcgac
     2881 caggcacacc accacgtact gtgggtgctc caccggcgcg aacgcggcga accacgacgc
     2941 gtcctgcagc ggcggcatct cggcggtgcc ggtcttgccg ccgaccgaca cctgggagaa
     3001 cgggaacccg aggaaggccc cggccgccgt gccgttcgtc acgaccccgc gcagcgactc
     3061 gcgcacgtag tccatctggc gcttggtgaa cggcagcttg ccggtcgagc gaggctggat
     3121 cttccgcacc agcgtgccgt ccggctccac caccttcagc gccaggcgcg gcgcccagag
     3181 cgtgcccccg ttcgcgatcg ccgagtaggc cgtcgccagc tgcagcggcg agacgagcac
     3241 gttcccctgg ccgaccgaca tgttgatgtc gtcgccgggg agccatcccg ttcggtcggc
     3301 gaagaacgcg ttgcgctgga tctccctctt cagccactcc acgtcgggga cgcgcccgcg
     3361 gtcctcgccg ggaaggtcga tccccgtgcg ccgccccagg ccgaac
//
LOCUS       DASPWO010000607         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1269092, whole genome shotgun sequence.
ACCESSION   DASPWO010000607 DASPWO010000000
VERSION     DASPWO010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269092"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="VEO00_13785"
     CDS             <1..1371
                     /locus_tag="VEO00_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59102.1"
                     /translation="RAGDIMFDTHSILRTDVFSAVAYGEPWLNQQWLAQIVLATAFRL
                     GGWFGLVALRALLMALVLSFVFLACRAAGAATKRAAWLTLASGVMLFGGFTLRPQLLG
                     MGCFAVTAWLVARRRTHPQGVWIVIPITILWANLHGSFFLAPLLLGLAWVEDRWVRGR
                     GARALLLAGLGSLLATTVTPYGFRVWSYAAGLATNPVIRRTITEWQPPSIGVYTGAAF
                     FLSVVAVAALLIARVRRPVPWGSLLSLVVFLAIALAAIRGVYWWAMAAPIVLAGVLEA
                     RPASTERRDPAGVLNAAIVGLLALAVIGSMIRWLPYTGPDMPTGGRLAFAPVGITREL
                     QRTLAPGEVVFNAQKWGSWLEFEFPSSPVIVDSLIEVIPSSVWWKYYAVSSGVDGWQE
                     TLDAWDVDVAVLARDQQGPLIPKVRADPGWRLVYEDAEGLIFRRVSDAAGAAGPADGA
                     ILRSSR"
     gene            complement(1275..>2103)
                     /locus_tag="VEO00_13790"
     CDS             complement(1275..>2103)
                     /locus_tag="VEO00_13790"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HYU59103.1"
                     /translation="GFLYTTIDLISIALAVWAVALVVRGRDRLGGVAMAAAILAKVWP
                     LVLLPVMVIQRKGRALVWSAAALALGTFVWVWWGGASGPLQVATQRQTPGWEYESLVG
                     SVFWALGRNFVIINDSTRVGFAPAWAKALLLGAALAGIAAVWYRASRREPRELGMPAV
                     AAVSILMLSAPLLSPPYMIWLAPWAAIAWMERRGYVRWLLAALMLVSGLMFLAFSSEF
                     LYYHAVWSVKAILLFRNALLVAVPAVYLLERRIAPSAGPAAPAASETRLKIRPSASS"
BASE COUNT          303 a          738 c          730 g          332 t
ORIGIN      
        1 cgtgccggcg acatcatgtt cgacacccac tcgatccttc ggacggacgt cttctcggcc
       61 gtcgcgtacg gcgagccctg gctcaaccag cagtggctgg cccagatcgt cctggcgacg
      121 gccttccggc tcggcgggtg gttcggtctg gtcgcgctcc gcgcgctgct catggccctc
      181 gtgctctcgt tcgtgttcct cgcgtgtcgt gcggctggtg cggccaccaa acgcgcggca
      241 tggctcacgc tggccagcgg cgtcatgctc ttcggcgggt tcacgcttcg accccaactc
      301 ctcgggatgg gctgtttcgc ggtgacggcg tggctggttg cacgtcgccg aacgcatccc
      361 caaggggtgt ggatcgtgat cccgatcacc atcctgtggg ccaacctgca cggcagcttc
      421 ttcctggctc cgctgctgct cggactggcg tgggtcgagg accgttgggt acgaggacgc
      481 ggggcgcgcg ccttgctctt ggccggcctc ggcagcctcc tcgcgacgac cgtgaccccg
      541 tacggcttcc gggtgtggtc ctacgccgcc ggtctggcga cgaatcccgt catccggaga
      601 acgatcacgg agtggcagcc tcccagcatc ggggtgtaca cgggggccgc gttcttcctg
      661 tcggtcgtgg cggtggcggc gctgctgatc gccagggtgc gccgacccgt cccctggggg
      721 tcgctgctgt ccctcgtggt gttcctggcg atcgcccttg ccgcgatccg cggcgtgtat
      781 tggtgggcca tggctgcgcc gatcgtcctt gccggcgtgc tggaagcgcg gccggcgtcc
      841 accgaacgcc gggaccccgc cggggtcctc aacgcggcga tcgtcggcct gctcgcgttg
      901 gccgtgatcg gaagcatgat ccggtggttg ccctacaccg gaccggacat gccgacggga
      961 ggcaggctcg ccttcgctcc ggtcgggatc acgcgggagc tgcagcggac gctggcgccg
     1021 ggggaggtcg tgttcaacgc gcagaagtgg ggctcgtggc tggagttcga gttcccgagc
     1081 agcccggtga tcgtcgattc gctcatcgag gtcatcccgt cgagcgtgtg gtggaagtac
     1141 tacgcggtct cgagcggcgt cgacggttgg caggagaccc tggatgcctg ggacgtcgac
     1201 gtggccgtcc tcgcgcgcga ccagcagggg ccgctcatcc cgaaggtgcg ggccgacccc
     1261 ggatggcggt tggtctacga ggatgccgag ggcctgatct tcaggcgagt ctccgacgcc
     1321 gccggggcgg cggggcccgc ggacggcgcg atccttcgct ccagcaggta gacggcgggg
     1381 accgccacga gcaacgcgtt acggaacaac aggatcgcct tcaccgacca cacggcgtgg
     1441 tagtagagga actccgaact gaacgcgagg aacatcaggc cgctcaccag catcagcgcg
     1501 gccagcagcc accgcacgta ccccctccgc tccatccagg cgatcgcggc ccatggggcg
     1561 agccagatca tgtacggggg cgacagcagc ggtgcggaca gcatgaggat cgacaccgcc
     1621 gccaccgccg gcatgccgag ctcccgcggc tcccgccggg atgcgcggta ccacaccgcc
     1681 gcgatcccgg ccagggccgc gccgagcaac agtgccttgg cccacgccgg cgcgaagccg
     1741 acgcgtgtcg agtcgttgat gatgacgaag ttgcgtccga gcgcccagaa gaccgagccg
     1801 accaggctct cgtactccca cccgggggtc tggcgctggg tcgccacctg cagcggaccc
     1861 gacgctccgc cccaccacac ccacacgaac gtgccgagcg cgagcgccgc tgccgaccag
     1921 acgagcgctc tccccttccg ctggatgacc atcaccggca acagcacgag cggccacacc
     1981 ttggccagga tggcggcggc catcgctacg ccgcccagcc gatcgcgccc tcgaaccacg
     2041 agcgcgaccg cccacaccgc gagcgcgatg gagatgaggt cgatggtcgt atacaggaag
     2101 ccg
//
LOCUS       DASPWO010000608         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1269216, whole genome shotgun sequence.
ACCESSION   DASPWO010000608 DASPWO010000000
VERSION     DASPWO010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269216"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VEO00_13795"
     CDS             complement(<1..649)
                     /locus_tag="VEO00_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59104.1"
                     /translation="MTLPPLPAFAVTGVGSLPDADVESAVDRVLAQAPDLPFAPQLPN
                     RSPRERPVAQAAEGLPGVRIDDRHGWAFVEGISIPGTGTVPEDSGEPVVGDEAYAGLY
                     RLLDRVKGSERPVKVQLTGPVTIASRVTDGRGRRAIYDSAWRRAAARAIGRKAAHLAD
                     QVRDAGCEPVVFLDEPELLGAAGPQAALSPAGAAALVAEALEEIDAPRGVHCCARV"
     gene            complement(646..1695)
                     /gene="mnmA"
                     /locus_tag="VEO00_13800"
     CDS             complement(646..1695)
                     /gene="mnmA"
                     /locus_tag="VEO00_13800"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HYU59105.1"
                     /translation="MSGGVDSSVSAALLHEAGYRVLGSHLKLVHMEGVDHGCCGPQAE
                     ADAAAVAERAGFDFEVVDLSETFERTVLADFVAEHAAGRTPNPCVRCNEHIKFRAFLE
                     RADALGFDLVATGHYVRADRGPDGAWRLLRGLDRAKDQSYVLHVLGQDQLARARFPVG
                     GMTKAETRAHARRLGLPVAGKPDSQEVCFVPDADHGAFLARLAPQLVRAGTMVDPAGN
                     VLREHDGTFRFTIGQRRHLGVSLPERAFVVDVDAAANRVVVGPESLLARRGLVAERVN
                     WIAGQPPVRGPFEAAARLRYRGEEVPTVVTPDPEAGTASLTFAAPQRAVAPGQSVAFY
                     RGDEVLGGGVIQSAR"
     gene            complement(1843..>2386)
                     /locus_tag="VEO00_13805"
     CDS             complement(1843..>2386)
                     /locus_tag="VEO00_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59106.1"
                     /translation="GFHTYPDLEIVEVVDPQTGAQVGEAEPGELVYTSLDWRGSALLR
                     YRTGIHARQGIVRAACPGCGRTVPRIGADLSRFEWAITVPSPTGPTPVDLADVIAVLW
                     GTNEVPLWQLDVLRSGLPAVPDGLTAHVAARPSTAVQVGRRLGRFGARVLPVAFRELR
                     GRVGVGLERSESRVVLRRAR"
BASE COUNT          319 a          925 c          875 g          267 t
ORIGIN      
        1 ccacgcgcgc gcagcagtgc acgccccgcg gcgcgtcgat ctcctccagc gcctcggcca
       61 ccagcgccgc cgcgccggcc ggagacagcg ccgcctgggg gccggccgcg ccgaggagct
      121 cgggctcgtc gaggaagacc accggctcgc agccggcgtc gcgcacctgg tccgccaggt
      181 gcgcggcctt ccggccgatg gcccgggccg ccgctcgccg ccaggcggag tcgtagatcg
      241 cccgccggcc gcggccgtcg gtgacgcgcg acgcgatggt caccggcccg gtgagctgca
      301 ccttgaccgg gcgctcgctc cccttcaccc ggtcgagcag ccggtacagg ccggcgtagg
      361 cctcgtcacc gaccaccggc tcgccgctgt cttccggcac cgtgccggtg ccggggatcg
      421 agatcccctc gacgaacgcc cagccgtggc ggtcatcgat gcggaccccg ggcagcccct
      481 cggccgcctg ggccaccggc cgctcccgcg gcgagcggtt cgggagctgc ggggcgaacg
      541 ggaggtccgg cgcctgggcc agcacacgat ccacggcgga ttccacgtcg gcgtccggca
      601 ggctcccgac gccggtgacg gcgaacgccg gcagcggcgg aagggtcatc gggcagactg
      661 gatgacgccg ccgcccagca cctcgtcccc gcggtagaag gcaacgctct ggccgggggc
      721 gacggcgcgc tgcggcgcgg cgaaggtcag gctcgcggtg cccgcttcgg gatcgggggt
      781 cacgaccgtc ggcacctcct cgccccggta gcggagtcga gcggcggcct cgaacggccc
      841 gcgcaccggc ggctgcccgg cgatccagtt cacgcgctcg gcgacgagcc cccggcgggc
      901 gagcagcgac tcgggcccga ccaccacccg gttcgcggcg gcgtcgacgt ccaccacgaa
      961 cgcccgctcc gggagcgaga cgccgaggtg gcggcgctgg ccgatcgtga accggaacgt
     1021 gccgtcgtgc tcgcggagca cgttcccggc cgggtcgacc atcgtccccg cgcggacgag
     1081 ctgcggcgcg agccgcgcga ggaacgcgcc gtggtcggcg tccggaacga agcacacctc
     1141 ctgcgagtcg ggcttcccgg ccaccggcag accgagccgc cgggcgtgcg cgcgggtctc
     1201 ggccttcgtc atcccgccga ccgggaaccg cgcccgcgcc agttggtcct ggcccagcac
     1261 gtgcaggaca tatgactggt ccttggcccg gtccaggccg cgcagcaggc gccacgctcc
     1321 gtcgggcccg cggtcggcgc gcacgtagtg cccggtggcc accaggtcga acccgagcgc
     1381 gtcggcgcgc tccaggaacg cccggaactt gatgtgctcg ttgcagcgca cgcaggggtt
     1441 cggcgttcgg ccggcggcgt gctcggcgac gaaatcggca aggacggtgc gctcgaatgt
     1501 ctcggagagg tcgaccacct cgaagtcgaa gccggcgcgc tcggcgacgg cggcggcgtc
     1561 ggcctcggcc tgtggtccgc agcacccgtg gtccaccccc tccatgtgga cgagcttcag
     1621 gtgcgacccg aggacccgat agcccgcctc gtgcagcagg gcggcggaca cggaggagtc
     1681 cacgccgccg gacatcgcca cgagcaccga cggggccgtc ggccgtcctt gaggggttcg
     1741 aacgccggac gtggtcgggc ctcgccgtcc ccagtgcgag ctaccccccc aaacccccgg
     1801 cggcgcgttc gccttcacga acgccgaggc tagcacgggt gatcagcggg cccggcgcag
     1861 cacgaccctg gactcagagc gctcgaggcc gacgcccacg cggccgcgca gctcgcggaa
     1921 cgccaccggc aacacccgcg ccccgaaccg gcccaggcgc cgcccgacct gcaccgccgt
     1981 cgacggccga gcggcgacgt gtgcggtcag cccgtccgga acggcgggca gcccgctgcg
     2041 cagcacgtcg agctgccaca gcggcacctc gttcgttccc cacaggacgg cgatcacgtc
     2101 ggcgaggtcc acgggggtcg ggccggtcgg cgacggaacg gtgatcgccc actcgaagcg
     2161 cgagaggtcg gccccgatcc gcggtaccgt gcgcccgcac ccggggcacg ccgcccgcac
     2221 gatcccctgg cgagcgtgga tcccggtccg gtagcggagc aaggcggagc cgcgccagtc
     2281 gagtgacgtg tacaccagct cacccggctc ggcctcgcct acctgcgcgc cggtctgcgg
     2341 gtcgacgacc tcgacgatct ccaggtccgg gtaggtgtgg aagccg
//
LOCUS       DASPWO010000609         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1186321, whole genome shotgun sequence.
ACCESSION   DASPWO010000609 DASPWO010000000
VERSION     DASPWO010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186321"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VEO00_13810"
     CDS             complement(<1..302)
                     /locus_tag="VEO00_13810"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HYU59107.1"
                     /translation="MAVDLHHTAATRLRAVGQRYTPNRRALVDILSAAAQPLSIPDVL
                     QAGPLLPQSSVYRNLAVLEQARVVRRIVASDEFARYELTEDLTGHHHHLICSNCGSV"
     gene            complement(308..1156)
                     /locus_tag="VEO00_13815"
     CDS             complement(308..1156)
                     /locus_tag="VEO00_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018828808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HYU59108.1"
                     /translation="MIAFALPWPFDRTYMQLALAAGLVVGAAAPLIGAFLVQKRMSLL
                     GDGIGHIAFAGVAGGLLLGVWPVWTALVVSVASALVIERLRARGTSGDLALALFFYGG
                     IAAGVVITGLAGSLNANLFAYLFGSILTVGRADVVVVVVLGVVIVATMAVTRRALFAV
                     VVDEEWARVAGLPVDLLNGILAALTAVTIVAAIRVVGVLLIAAMIVLPVACARLVARS
                     FRRQLTWSMVIGMGAALVGLIAARAWALAPGGSIVLTAVLTYAVLAIATVRRRRSQRF
                     SIRMVQ"
     gene            complement(1153..1917)
                     /locus_tag="VEO00_13820"
     CDS             complement(1153..1917)
                     /locus_tag="VEO00_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HYU59109.1"
                     /translation="MPLTAGRDLIVRLRGVTFGYGREPVVEDVSLSIARGEFVALVGP
                     NGSGKSTLLRVLLGLLEPQAGRVELFGQTPAELREPWRVGYVPQRPALAADLPATVEE
                     VVAGGRLARVGWWRRLRAADREAVAHAMDSVALLALRDRPVGELSGGQQQRAFIARAL
                     AGEPQLLVLDEPIAGIDAESQRRFRESLSHLIREHDAAVLLVSHELGAVAEDLDRVIV
                     LKRRVLFDGPPPELMAAGVSLGVHREDLPLWLERLG"
     gene            complement(1908..>2759)
                     /locus_tag="VEO00_13825"
     CDS             complement(1908..>2759)
                     /locus_tag="VEO00_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HYU59110.1"
                     /translation="VAACSSSDGGAGAGKTSVVASFYPLEEAATRIGGDRVAVTNLTA
                     PGVEPHDLELNPDQVDAILDADVVLYLGSGFQPAVEDALADAGGEAVDLLEGFPLAES
                     ADEEEHGPDPHVWLDPTLMARIARRVRDALGEADPGGASTFGSNAETYVSELGELDEA
                     FQSGLTGCARDLIVAAHAAFGYLAARYDLRQEAITGISPEAEPDPARLAELVDLVERE
                     GVTTIFTEELVSPEVAETLAREAGVTTDVLNPLEGLTPDEVAAGDDYASFMRENLEAL
                     RRALGCP"
BASE COUNT          421 a         1038 c          975 g          325 t
ORIGIN      
        1 accgagccgc agttcgagca gatcaggtgg tggtggtgac cggtcaggtc ctccgtcagc
       61 tcgtagcgcg cgaactcgtc gctcgccacg atccgccgca cgacgcgcgc ctgctccagc
      121 accgcgaggt tgcggtacac cgatgactgg gggagtaacg ggccggcctg cagcacgtcg
      181 gggatggaga ggggctgggc ggccgcggag aggatgtcca cgagcgcccg ccggttcggc
      241 gtgtagcgct gacccacggc gcgcaggcgg gtcgcggcgg tgtggtggag gtcgacggcc
      301 acggcggtca ctgtaccatc cggattgaga accgctgaga acggcgccgg cggacggtgg
      361 cgatcgccag gacggcgtac gtgagcacgg cggtgagcac gatcgaaccc cccggcgcca
      421 gcgcccacgc ccgcgccgcg atcagcccga cgagcgccgc gcccatcccg atcaccatcg
      481 accacgtcag ctggcgccgg aacgagcgag ccacaagccg cgcgcacgcc accgggagca
      541 cgatcatcgc cgctatcagc aggaccccga ccacgcggat cgcggcgacg atcgtcaccg
      601 ccgtcagcgc cgcgaggatc ccgttcagca ggtccaccgg aaggccggcg acccgcgccc
      661 attcctcgtc caccaccacc gcgaacagcg cccgcctcgt caccgccatc gtcgccacga
      721 tcacgacacc gagcaccacg accacgacga cgtcggcgcg tccgaccgtc aggatcgagc
      781 cgaacaggta ggcgaacagg ttggcgttca gcgatccggc cagcccggtg atgaccacgc
      841 cggcggcgat gccgccgtag aagaacaggg cgagggcgag gtctccggac gtgccgcgcg
      901 cgcgcaggcg ctcgatcacg agcgcgctgg ccaccgacac gaccagggcc gtccacacgg
      961 gccagacccc gagcagcagc ccgcccgcga cgccggcgaa cgcgatgtgg ccgatcccgt
     1021 cgcccaagag cgacatccgc ttctggacca ggaacgctcc gatcaggggt gccgccgcac
     1081 cgaccaccag gccagccgcc agcgccagct gcatgtaggt gcggtcgaag ggccagggga
     1141 gggcgaacgc gatcatccca gtcgctccag ccagagcggg aggtcctcgc ggtgcacgcc
     1201 gaggctgacc ccggcggcca tcagctccgg gggcgggccg tcgaacagta cccgccgctt
     1261 caggacgatc acccggtcga gatcttccgc gacggcgccc agctcgtgcg acacgagcag
     1321 caccgcagcg tcgtgctcac ggatcaggtg gctgagcgat tcacggaacc ggcgctgcga
     1381 ctcggcgtcg atcccggcga tcggctcgtc gagcaccagg agctggggct ccccggccag
     1441 cgctcgggcg atgaacgcgc gctgttgctg cccaccggag agctcgccga ccggtcggtc
     1501 gcgtagcgcg aggagcgcca cggagtccat ggcgtgggcg accgcctccc gatccgccgc
     1561 ccggagccgg cgccaccagc ccacgcgtgc cagccggccg ccggccacga cctcctccac
     1621 cgtcgcgggg aggtctgcgg ccagggccgg acgctggggg acgtacccca cgcgccacgg
     1681 ctcgcgcagc tccgccggcg tctgcccgaa cagctccacc cggccggcct gtggctcgag
     1741 cagcccgagc aggacccgca gcagggtgga cttcccggag ccgttcggtc cgacgagggc
     1801 gacgaattcg ccgcgggcga tcgataggga gacgtcctcg accacgggct cgcgcccgta
     1861 gccgaaggtc acgccccgca ggcggacgat gaggtcgcgc cccgcggtca ggggcacccc
     1921 agcgctcgcc gcagcgcctc gaggttctcg cgcatgaacg aagcgtaatc gtcgcccgcc
     1981 gctacctcgt ccggggtcag gccctcgagc ggattgagca cgtcggtcgt cacgccggcc
     2041 tcgcgcgcca gcgtctcggc gacctcgggc gagacgagct cctcggtgaa gatcgtggtg
     2101 acgccctcgc gctcgaccag gtcgaccagc tcggccaggc gcgccggatc gggttccgcc
     2161 tccggcgaga tgccggtgat cgcctcctgg cggaggtcgt agcgggccgc gaggtagccg
     2221 aacgcggcgt gcgcggcgac gatcaggtcc cgcgcgcacc cggtcaggcc cgactggaac
     2281 gcctcgtcga gctcgcccag ctcggagacg tacgtctccg cgttgctccc gaatgtcgac
     2341 gcgcctccgg ggtcggcctc gcccagggcg tcgcgaacgc gccgggcgat gcgggccatc
     2401 agggtggggt ccagccagac gtgggggtcg gggccgtgct cttcctcgtc ggcgctctcc
     2461 gcgagcggga acccctcgag caggtcgacc gcctcgccgc cggcgtccgc cagcgcgtcc
     2521 tccacggccg gctggaaccc gcttcccagg tacagcacga cgtcggcgtc caggatcgcg
     2581 tccacctggt ccgggttcag ctcgaggtcg tgcggctcga cccccggcgc tgtcaggttg
     2641 gtgacggcga cgcggtctcc gccgatccgg gtggcggcct cctccagggg atagaagctc
     2701 gccaccacgg acgtcttccc cgcgccggcg ccgccgtccg agctggagca cgccgccac
//
LOCUS       DASPWO010000610         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1017032, whole genome shotgun sequence.
ACCESSION   DASPWO010000610 DASPWO010000000
VERSION     DASPWO010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017032"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VEO00_13830"
     CDS             complement(<1..330)
                     /locus_tag="VEO00_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYU59111.1"
                     /translation="MIAFPAAAAVVAFVFAFSLGRRYLVARRPYLALWAVALAMYAVA
                     SAALALGVLGTWSAAEFETYWLFGAVLNVPYLAAGEVYLLAKRPWVARLVLAAVVAAS
                     VAATVIVF"
     gene            complement(353..1915)
                     /locus_tag="VEO00_13835"
     CDS             complement(353..1915)
                     /locus_tag="VEO00_13835"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HYU59112.1"
                     /translation="MSGAPGNVADLVRRAAERAGSRPALLFRGRPVSHAGLDERVDRV
                     AAALAGMGIRPGDRVALLLGNVPEFVYAFYGALRAGAVVAPLNVMLTAHELGYILADA
                     GARAIVAEIGYLPSILAVRDRLPGLELVLVVGGPPAPPGTVSFEEALGRAGNPPDTAA
                     GGDDLAVIAYTSGTTAQPKGAMLSHRNLLANLEQTAAVPALASREDDVVLVVLPLFHI
                     YALNAVLGPTIDAGGAAVLVERFDPAETLDLVERHGITVLPGAPPMFQAWLALEGDAR
                     DLSSVRLAVSGAAPLPGEVLEAFRERFGVTIWEGYGLTESAPAVSSNAAGPVAKPNSI
                     GLPLPGVEVRLVDERGEDVEEGDPGEIVVRGPNVFRGYWGRQGESEAVLRGQWLRTGD
                     VAYRDEDGYLFIVDRVKDLIIVSGFNVFPKEVEEALAAHPKVAEAAVVGVKDERTGEA
                     VKALVVLNPGTNATEEELIDWCRVSLARFKCPRVVEFVDELPKHATGKVLRRALRGEE
                     VLGGTGERAPAP"
     gene            complement(1929..>2714)
                     /locus_tag="VEO00_13840"
     CDS             complement(1929..>2714)
                     /locus_tag="VEO00_13840"
                     /inference="COORDINATES: protein motif:HMM:NF025608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HYU59113.1"
                     /translation="RWDLDFGIGDPTYRILPDALYRSSLAPTKPFVPNPDARFSGTLE
                     VDGETVRLEGAPGQQGHLHGERHAERWAWVHASGFEGADGVVLEGLSAQGRRGPLRTP
                     HTTFVGLRWGGEWLRFGRASRRRGWDLGRWRIDLASRSHHLFGTVTAGAGTLVRARYE
                     DPDGTERFCHNSTVASVHLTLLEKTTGGYEELANLVADGTAQAEWAGRTPAPAATAVH
                     QPIDEPEPATPVGGGSDRRRRSGRSRPRARYPPEPPGGDATHR"
BASE COUNT          370 a         1046 c          984 g          314 t
ORIGIN      
        1 gaatacgatc acggtcgccg ccacgctggc cgccaccacg gccgcgagca ccagccgcgc
       61 gacccacggg cgcttcgcca ggaggtacac ctccccggcg gcgaggtacg gcacgttcag
      121 caccgccccg aacagccagt acgtctcgaa ctccgccgcc gaccacgtcc cgagcacgcc
      181 gagcgccagc gcggccgagg cgacggcgta catcgccagc gccaccgccc acagcgccag
      241 gtacgggcgg cgcgccacga ggtagcggcg ccccagggag aacgcgaaga cgaacgcgac
      301 gaccgcggcg gcggccggga aggcgatcat gggcgagcgg ggcgcgggac gctcacggtg
      361 ccggcgcgcg ctcgccggtg ccgcccagga cctcctcgcc gcgcagggcg cgccggagca
      421 ccttcccggt ggcgtgcttg ggcagctcgt cgacgaactc gaccacgcgc gggcacttga
      481 accgggccag cgagacgcgg caccagtcga tcagctcctc ctcggtggcg ttcgtccctg
      541 ggttcaggac gacgagggcc ttcacggcct cgccggtgcg ctcgtccttg accccgacca
      601 ccgcggcctc ggccaccttc gggtgcgcgg cgagggcctc ctcgacctcc ttcgggaaca
      661 cgttgaaccc ggacacgatg atcaggtcct tcacccggtc gacgatgaac aggtagccgt
      721 cctcgtcccg gtaggcgacg tcgcccgtgc gcagccactg cccgcgcagg accgcctcgc
      781 tctcaccctg ccgcccccag tacccgcgga agacgttcgg cccgcggacg acgatctccc
      841 ccgggtcgcc ctcctcgacg tcctcaccgc gttcgtccac caggcggacc tcgacgcccg
      901 ggagcggcag cccgatcgag ttcggcttcg ccacgggccc ggccgcgttc gacgagacgg
      961 cgggcgcgga ctcggtcagt ccgtagccct cccagatcgt gacgccgaag cgctcccgga
     1021 aggcctccag gacctcgccc gggagggggg cggcccccga gacggccagg cgcaccgagg
     1081 agaggtcgcg ggcgtcgccc tcgagcgcca gccaggcctg gaacatcggc ggcgcgccgg
     1141 ggagcacggt gatcccgtgc cgctcgacca ggtcgagcgt ctcggccggg tcgaagcgct
     1201 cgacgagcac ggccgcgcct ccggcgtcga tcgtgggccc gagcaccgcg ttcagcgcgt
     1261 agatgtggaa cagcggcagc accacgagga ccacgtcgtc ctcgcgcgac gccagcgccg
     1321 ggaccgccgc cgtctgctcc aggttcgcca gcaggttgcg gtgggagagc atcgcgccct
     1381 tcggctgcgc ggtggtcccg gacgtgtacg cgatcacggc gaggtcatcg ccgccggcag
     1441 cggtgtcggg agggttgccg gcccgcccca gcgcctcctc gaacgagacc gtgccggggg
     1501 gcgccggcgg accaccgacc acgaggacga gctccaggcc cgggaggcgg tcgcgcaccg
     1561 cgaggatcga aggaaggtag ccgatctcag cgacgatcgc ccgcgcgccg gcgtcggcca
     1621 ggatgtagcc gagctcgtgt gccgtgagca tcacgttcag cggcgcgacc accgcccccg
     1681 cccggagggc cccgtagaag gcgtagacga actcggggac gttcccgagc aggagcgcca
     1741 cgcggtctcc cggccggatc cccatgccgg ccagcgcggc ggcgacgcgg tccacccgct
     1801 cgtcgagccc ggcgtgggac accgggcgac cccggaacag cagcgccgga cggctcccgg
     1861 cccgttcggc agcgcgacgg acgaggtcgg ccacgttccc cggagcgccg ctcatgtccg
     1921 cggcgagtct accggtgggt cgcgtcgccg ccggggggct cgggggggta cctcgccctg
     1981 ggacgagatc tgcccgaccg cctccggcgg tcggaccccc cgcctacagg agtggcgggc
     2041 tccggctcgt cgatcggctg gtgcaccgcc gtggccgccg gcgccggggt ccgcccggcc
     2101 cactcggcct gcgcggtccc gtcggcgacc aggttcgcca gctcctcgta gccgccggtg
     2161 gtcttctcga ggagcgtcag gtgcacggag gcgaccgtcg agttgtggca gaagcgctcg
     2221 gtgccgtcgg ggtcctcgta gcgcgcccgg accagggtcc cggcgccggc cgtgacggtg
     2281 ccgaacaggt ggtggctccg gctcgccagg tcgatcctcc agcgcccgag gtcccagccc
     2341 cgccggcgcg aggcgcgccc gaaccgcaac cactccccgc cccagcgcag cccgacgaac
     2401 gtggtgtgcg gcgtgcgcag cggcccgcgc cgcccctggg ccgacagtcc ctcgagcacg
     2461 acgccgtcgg cgccctcgaa cccgctggcg tgcacccagg cccagcgctc ggcgtgtcgc
     2521 tcgccgtgca ggtgcccctg ctggccgggg gctccctcca gccgcacggt ctcgccgtcg
     2581 acctcgagcg tgcccgagaa ccgggcgtcg gggttcggca cgaacggctt cgtcggcgcg
     2641 agcgagctgc ggtacagggc atcggggagg atgcgatacg tggggtcgcc gatgccgaag
     2701 tcgaggtccc agcg
//
LOCUS       DASPWO010000611         2839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U11543
            k141_1187437, whole genome shotgun sequence.
ACCESSION   DASPWO010000611 DASPWO010000000
VERSION     DASPWO010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945273
            Sequence Read Archive: SRR6266508
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 06:37:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,769
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,698
            CDSs (with protein)               :: 2,698
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 11 of 45
            Pseudo Genes (incomplete)         :: 37 of 45
            Pseudo Genes (internal stop)      :: 2 of 45
            Pseudo Genes (multiple problems)  :: 5 of 45
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187437"
                     /isolate="SMAG_U11543"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..>2839
                     /locus_tag="VEO00_13845"
     CDS             150..>2839
                     /locus_tag="VEO00_13845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HYU59114.1"
                     /translation="MSIEPAFDDRDSRRVLDNDAGTLLVTGAPGTGKSTLLAERFCRL
                     VTGGADPERVLFLTLTRRGREAARRRIVSQLGRSVADLPLLTFHGYALRVLGEHWEAL
                     GFERLPTLLTAPEQYRRTSELLRAEAPALWPALDAYRSMKRFAREVADFVLRAQERLL
                     RPPDLAELAERAGRPDLAEVAAFYDRYLDVLEPANEVDFAGALRLAVRAAGEGILTLP
                     EHLLVDDLQDVTSAGGELVARLEAGATTAVLAADPDGRVFSFRGGSGESLDRLQARAS
                     VPHLVLEGSHRLAPEVDAVCRSLTADRRDPVEGVERWCFTQPADEADAVARELLREHL
                     ERATPWGEMAVILRSLGTYLPVLRRALTHRGVPFVAVGESSVITSEPAVRPIAQLLQF
                     ALRPERREELVEALLVSPVVGLHPHDVRALRRAAKLATPPTTVLAVAEGPRALDPEIR
                     ARLEAFLGLVADVVRWNDERSPDGVLYEVWRRLPHLAALVEGAERDESDGDAQRDLDA
                     LYAFANVVARFAERQGGGATVLEYLDALEEAEFGADPFVLPEERSPDAVRVVTAHRAH
                     GEEFAVAFVVGCIEGQFPQLSSSRPLVDLDDLTGPRSPMDRYRERFREERALFRLAIS
                     RGRRLVLTASEARDSRRPRTPTRFLPGPWGDGPADRSPVSLAQAEAEHRRALASNEPA
                     PRRLAALAALAALSARPDTWWGAREWTAGPPLHPGEFKTSYSRLSTLENCALQYLFQQ
                     ELGLDPSSSHHAWLGGRVHQVIERFWRGEIERTQEAMEADLDAHWRPEAFPSRAVEHR
                     RRLDASEMFRKFLANEAPVEPALVEHQFHFPIDGALLRGRIDAVIATPDGVRILDYKT
                     GRHAIEQQTAKDDLQLGTYLLAARRDDTIRALGNPKRLELV"
BASE COUNT          342 a         1103 c         1022 g          372 t
ORIGIN      
        1 cactccggcc ccggccacga cggggacggc gagggcgggg acgactccgg gagccgctga
       61 tcgccgtcga tcgccaccga aggggccggc gcgcccggcc ccttcgccgt tccggggccg
      121 tgtcggaccc ccggagtagc atcggcttga tgagcatcga gcccgcgttc gacgaccggg
      181 actcccggcg cgtcctcgat aacgacgccg ggacgctgct cgttaccggc gcaccgggaa
      241 ccggcaagtc aacgctcctc gcggagcgct tctgccggct cgtcaccggc ggcgccgacc
      301 ccgagcgcgt cctgttcctg acgctcaccc gccgggggcg cgaggccgcc cggcgccgga
      361 tcgtctccca gctcgggcgc agcgtggccg acctgccgct gctcacgttc cacgggtacg
      421 cactccgcgt cctcggcgag cactgggagg ccctcggctt cgagcgactg ccgacgctgc
      481 tgacggcacc cgagcagtac cggcggacgt ccgaactgct gcgggccgag gcgccggcgc
      541 tgtggccggc cctggacgcc taccgctcga tgaagcgctt cgcccgcgag gtggccgatt
      601 tcgtgctccg ggcgcaggag cggctgctgc gcccgcccga cctggcggag ctggccgagc
      661 gcgccggccg gccggacctg gccgaggtgg ccgccttcta cgaccgctac ctggacgtcc
      721 tcgagccggc gaacgaggtc gacttcgccg gagcgctgcg gctcgccgtc agggcggccg
      781 gcgagggcat cctgacgctg cccgagcacc tcctggtgga cgacctgcag gacgtcacct
      841 ccgccggcgg cgagctggtg gcccgcctcg aggcgggcgc gacgaccgcc gtcctcgccg
      901 ccgaccccga cggccgggtc ttctcctttc ggggcgggtc gggcgagtcg ctggaccggc
      961 tgcaggcccg cgcgtcggtc ccgcacctgg tcctcgaggg ctcgcatcgg ctggccccag
     1021 aggtcgacgc cgtgtgccgt tcgctgacgg ccgaccgccg tgacccggtg gaaggggtgg
     1081 agcgatggtg cttcacgcag ccggccgacg aggccgacgc ggtcgcccgc gagctgctcc
     1141 gcgaacactt ggagcgggcc acgccctggg gcgagatggc cgtgatcctg cggagcctgg
     1201 ggacgtacct gccggtgctg cggcgggcgc tgacccaccg cggcgtgccg ttcgtggcgg
     1261 tcggcgagtc ctcggtgatc acctccgagc cggccgtgcg cccgatcgcc cagctgctgc
     1321 agttcgcgct gcgccccgag cggcgggagg aactggtcga ggccctgctc gtctccccgg
     1381 tggtcggcct gcacccgcac gacgtccgcg ccctgcgccg cgcggcgaag ctggccacgc
     1441 ctccgaccac ggtcctcgcc gtcgccgagg gtccgcgggc gctcgacccg gagatccgcg
     1501 cgaggctcga ggcgttcctc ggcctcgtcg ccgacgtcgt ccgctggaac gacgagcgct
     1561 cccccgatgg cgtcctgtac gaggtgtggc ggcgcctgcc ccacctcgcc gccctggtcg
     1621 agggggccga gcgagacgag tccgacggcg acgcgcagcg cgacctcgac gcgctgtacg
     1681 ccttcgccaa cgtggtggcc cgcttcgcgg aacgtcaggg cggcggcgcc acggtcctcg
     1741 agtacctcga cgccctcgag gaggcggagt tcggcgccga cccgttcgtg ctgccggagg
     1801 agcggtcgcc cgacgcggtg cgcgtggtca ccgcccatcg cgcgcacggc gaggagttcg
     1861 cggtcgcctt cgtcgtcggg tgcatcgagg gccagttccc gcagctctcc tcgtcacggc
     1921 ccctggtcga cctggacgac ctgaccggcc cgcgctcgcc gatggaccgc taccgggaac
     1981 gcttccgcga ggaacgggcg ctgttccgcc tggcgatctc gcgcggccgg cggctggtcc
     2041 tgaccgccag cgaggcgcgc gactcgcgcc gcccgcgcac cccgacccgc ttcctcccag
     2101 ggccctgggg tgacggcccg gccgaccgga gcccggtgtc gctcgcccag gccgaggccg
     2161 agcaccgccg cgcgctcgcc tcgaacgagc ccgccccgcg gcgcctggcc gccctcgccg
     2221 ccctggccgc cctcagcgcc cgccccgaca cctggtgggg cgctcgtgag tggaccgccg
     2281 gcccgccgct gcaccccggc gagttcaaga cgtcgtacag ccgcctgtcg accctggaga
     2341 actgcgcgct gcaatacctg ttccagcagg agctcggcct cgacccgtcc agcagccacc
     2401 acgcctggct cgggggccgt gtgcaccagg tgatcgagcg cttctggcgc ggcgagatcg
     2461 agcggaccca ggaggcgatg gaggccgacc tcgacgcgca ctggcggccg gaggcctttc
     2521 ccagccgggc ggtcgagcac cgccgacggc tcgacgcgtc cgagatgttc cgcaagttcc
     2581 tcgcgaacga ggcgcccgtc gagccggcgc tcgtcgagca ccagttccac ttcccgatcg
     2641 acggcgcgct cctgcgaggc cggatcgacg ccgtgatcgc cacgccggat ggcgttcgga
     2701 tcctcgacta caagaccggc cggcacgcca tcgagcagca gaccgcgaag gacgacctgc
     2761 agctcgggac ctacctgctg gccgcgcgcc gagacgacac gattcgtgcc ctcgggaacc
     2821 ccaagcggct cgagctcgt
//
